From PAMarcum <@t> uams.edu Mon Feb 2 08:22:58 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Feb 2 08:23:12 2015 Subject: [Histonet] ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH Message-ID: <56d5892a005f4deaaf2cfed127d975b9@MAIL13M2N2.ad.uams.edu> Good Morning All, THE ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH, 2015 IN LITTLE ROCK, ARKANSAS PLEASE JOU US!! The meeting is being held at the Baptist Schools of Health in Little Rock and is open to anyone in Histology and related or interested fields. The following list is for the speakers, who will all be presenting 90 minute seminars for the meeting. We will have two rooms of speakers so you have a choice in classes and topics for the day. 7:30AM Registration 8AM to 9:30AM Seminars Dr Ericka Olgaard from UAMS Diagnostic Challenges of Breast Cancer Dr Daisy Alapat UAMS Methods and Basics of Protein Detection in Hematopathology 9:30AM to 10:00AM BREAK 10:00AM to 11:30AM Seminars Dr Shree Sharma Nephropath Integrating Mobile Technology With Anatomic Pathology Debbie Siena Technical Specialist Statlab Controlling Your Special Stains 11:30AM to 1:00PM Lunch 1:00PM to 2:30PM Seminars Dr Sara Shalin UAMS How and Why Skin Is Different Jennifer Feldman Ventana Antibody Production 2:30PM to 3:00PM Break 3:00PM to 4:30PM Seminars Dr Jennifer Forsythe ME Little Rock Autopsy in America Pamela Marcum HT UAMS Reagent Alcohol - Can't Drink It - What Is It? Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Richard.Cartun <@t> hhchealth.org Mon Feb 2 10:52:40 2015 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Feb 2 10:52:47 2015 Subject: [Histonet] "Know Error" Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3E0E26AE@HHCEXCHMB03.hhcsystem.org> Our laboratory has a new urology group who is demanding that we send-out every "Positive" (with CA) prostate biopsy for the "Know Error" DNA identity test. The test is used to confirm the identity of the patient diagnosed with cancer by matching the DNA in the cancer cells to that obtained from a buccal swab from the patient obtained in the doctor's office. In my opinion, this test is absolutely unnecessary; another expense ($1,700 list price) to our healthcare system that is not justified. In addition, the laboratory ends up doing a lot of work that it cannot get reimbursed for. However, I would like to hear from those of you who have experience with it. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tejohnson <@t> genoptix.com Mon Feb 2 12:13:33 2015 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Mon Feb 2 12:13:45 2015 Subject: [Histonet] RE: "Know Error" Message-ID: That sounds like a valid concern, Richard. Thank you for being an advocate for good utilization of patient testing. What is behind their concern? Do they think there has been a mistake with patient identification in your lab? Or are they trying to reconcile low PSA or other discordant screening test results with a diagnosis of prostate CA? I would offer to send whatever they need from the lab (paraffin section curls, unstained slides, etc) back to their office, so long as they provide patient consent. The urologist's office can then package that material with the buccal swabs they get from the patient. The onus can be on them to manage the cost of the testing, and they can then try to justify it with the patient when insurance will not reimburse for it. Is this option feasible? Teri ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From CDavis <@t> che-east.org Mon Feb 2 12:24:02 2015 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Mon Feb 2 12:22:40 2015 Subject: [Histonet] QC for IHC bulk solutions Message-ID: Good afternoon Histoland folks, other than documenting the ph of each Ventana bulk solution after diluting and visual inspections of slides run with that solution lot what other way is there to QC the EZ Prep, Reaction Buffer, and Liquid Coverslip bulks solutions? (we currently have a lively dialog going on about this) There is always something to do and something new in Histology... Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From akbitting <@t> geisinger.edu Mon Feb 2 14:48:15 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Mon Feb 2 14:48:26 2015 Subject: [Histonet] Benchmark Special Stainers Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7E3DB1@GHSEXMBX1W8K1V.geisinger.edu> Can anyone tell me if the waste collection on the Benchmark Special Stainer is the same as the waste collection on the Benchmark Ultra or XT? Carboy with spigot?? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From staceyjm <@t> sbcglobal.net Mon Feb 2 14:56:49 2015 From: staceyjm <@t> sbcglobal.net (STACEY) Date: Mon Feb 2 14:56:59 2015 Subject: [Histonet] Benchmark Special Stainers Message-ID: <1422910609.7337.YahooMailBasic@web181402.mail.ne1.yahoo.com> Yes, it is the same. (Carboy with a spigot.) Stacey Merica, B.S., HT Histology Supervisor North Kansas City Hospital North Kansas City, MO 64116 ------------------ On Mon, 2/2/15, Bitting, Angela K. wrote: Subject: [Histonet] Benchmark Special Stainers To: "histonet@lists.utsouthwestern.edu" Date: Monday, February 2, 2015, 2:48 PM Can anyone tell me if the waste collection on the Benchmark Special Stainer is the same as the waste collection on the Benchmark Ultra or XT? Carboy with spigot?? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Mon Feb 2 15:00:13 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Mon Feb 2 15:00:20 2015 Subject: [Histonet] Benchmark Special Stainers In-Reply-To: <1422910609.7337.YahooMailBasic@web181402.mail.ne1.yahoo.com> References: <1422910609.7337.YahooMailBasic@web181402.mail.ne1.yahoo.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7E3E0A@GHSEXMBX1W8K1V.geisinger.edu> Thank you everyone for your swift replies. -----Original Message----- From: STACEY [mailto:staceyjm@sbcglobal.net] Sent: Monday, February 02, 2015 3:57 PM To: Bitting, Angela K.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Benchmark Special Stainers Yes, it is the same. (Carboy with a spigot.) Stacey Merica, B.S., HT Histology Supervisor North Kansas City Hospital North Kansas City, MO 64116 ------------------ On Mon, 2/2/15, Bitting, Angela K. wrote: Subject: [Histonet] Benchmark Special Stainers To: "histonet@lists.utsouthwestern.edu" Date: Monday, February 2, 2015, 2:48 PM Can anyone tell me if the waste collection on the Benchmark Special Stainer is the same as the waste collection on the Benchmark Ultra or XT? Carboy with spigot?? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llang <@t> aipathology.com Mon Feb 2 15:13:19 2015 From: llang <@t> aipathology.com (LeAnn Lang) Date: Mon Feb 2 15:13:32 2015 Subject: [Histonet] Lean Management Message-ID: I am looking for some great programs for lean management in a laboratory setting (both cytology and histology). Can anyone help me out? Thank you, LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com From Lacie.Algeo <@t> providence.org Mon Feb 2 16:13:48 2015 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Mon Feb 2 16:13:59 2015 Subject: [Histonet] QIHC Message-ID: <24C4B3C167E5694887AB594C7602CE3A03B860B6@WN35104.or.providence.org> Hi All, What are the best methods for studying for the QIHC? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From BDeBrosse-Serra <@t> isisph.com Mon Feb 2 17:20:00 2015 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Feb 2 17:20:23 2015 Subject: [Histonet] RE: QIHC In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A03B860B6@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A03B860B6@WN35104.or.providence.org> Message-ID: Dako IHC booklet. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Monday, February 02, 2015 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hi All, What are the best methods for studying for the QIHC? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chesarato <@t> hotmail.com Mon Feb 2 21:30:52 2015 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Mon Feb 2 21:30:58 2015 Subject: [Histonet] RE: Know Error Message-ID: I live in a Corrupt Country . I can assure to you that this request is simply that, Corruption. The well done job is not well paid. The one that returns money to a corrupt person or a group of persons , yes, it is well paid. C?sar RomeroBuenos AiresArgentina From Fawn.Bomar <@t> HalifaxRegional.com Tue Feb 3 07:48:20 2015 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Tue Feb 3 07:48:44 2015 Subject: [Histonet] Storage of paraffin blocks Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B97E55F5@EXCH-2K10.hrhs.com> Hi everyone, I hope all is going well in the new year. I was wondering if everyone that stores their paraffin blocks in the plastic tissue tek block filing cabinets, stores these cabinets directly on the floor or do you all place them on some sort of stand? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From Toni.Rathborne <@t> rwjuh.edu Tue Feb 3 07:58:02 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Tue Feb 3 07:58:11 2015 Subject: [Histonet] RE: Storage of paraffin blocks In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B97E55F5@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B97E55F5@EXCH-2K10.hrhs.com> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4264A@vap1014.win.rwjuh.edu> To comply with Joint Commission standards, ours are stored 6" off the floor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Tuesday, February 03, 2015 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of paraffin blocks Hi everyone, I hope all is going well in the new year. I was wondering if everyone that stores their paraffin blocks in the plastic tissue tek block filing cabinets, stores these cabinets directly on the floor or do you all place them on some sort of stand? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CArnold <@t> opko.com Mon Feb 2 12:37:30 2015 From: CArnold <@t> opko.com (Casey Arnold) Date: Tue Feb 3 08:16:14 2015 Subject: [Histonet] RE: Know Error Message-ID: We have many clients who perform "Know Error" for their patients. What we do is send out only one site with malignancy (preferably the highest grade) instead of all sites. This cuts down on a lot of unnecessary work to be done by the tech and cuts costs for you. I find it easiest and most cost effective to send them the vial of tissue instead of the slides. I also believe that if you contact a Know Error representative you should be able to develop a contract to ensure reimbursement. Hope this helps! Casey Arnold Histology Supervisor 1450 Elm Hill Pike Nashville, TN? 37210 Office:? 615.874.0410 Cell:? 615.714.2940 Fax:? 615.345.4595 carnold@opko.com www.opko.com NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information.? Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, February 02, 2015 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH (Marcum, Pamela A) 2. "Know Error" (Cartun, Richard) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Feb 2015 14:22:58 +0000 From: "Marcum, Pamela A" Subject: [Histonet] ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH To: "histonet@lists.utsouthwestern.edu" Message-ID: <56d5892a005f4deaaf2cfed127d975b9@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Good Morning All, THE ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH, 2015 IN LITTLE ROCK, ARKANSAS PLEASE JOU US!! The meeting is being held at the Baptist Schools of Health in Little Rock and is open to anyone in Histology and related or interested fields. The following list is for the speakers, who will all be presenting 90 minute seminars for the meeting. We will have two rooms of speakers so you have a choice in classes and topics for the day. 7:30AM Registration 8AM to 9:30AM Seminars Dr Ericka Olgaard from UAMS Diagnostic Challenges of Breast Cancer Dr Daisy Alapat UAMS Methods and Basics of Protein Detection in Hematopathology 9:30AM to 10:00AM BREAK 10:00AM to 11:30AM Seminars Dr Shree Sharma Nephropath Integrating Mobile Technology With Anatomic Pathology Debbie Siena Technical Specialist Statlab Controlling Your Special Stains 11:30AM to 1:00PM Lunch 1:00PM to 2:30PM Seminars Dr Sara Shalin UAMS How and Why Skin Is Different Jennifer Feldman Ventana Antibody Production 2:30PM to 3:00PM Break 3:00PM to 4:30PM Seminars Dr Jennifer Forsythe ME Little Rock Autopsy in America Pamela Marcum HT UAMS Reagent Alcohol - Can't Drink It - What Is It? Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Mon, 2 Feb 2015 16:52:40 +0000 From: "Cartun, Richard" Subject: [Histonet] "Know Error" To: "histonet@lists.utsouthwestern.edu" Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3E0E26AE@HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Our laboratory has a new urology group who is demanding that we send-out every "Positive" (with CA) prostate biopsy for the "Know Error" DNA identity test. The test is used to confirm the identity of the patient diagnosed with cancer by matching the DNA in the cancer cells to that obtained from a buccal swab from the patient obtained in the doctor's office. In my opinion, this test is absolutely unnecessary; another expense ($1,700 list price) to our healthcare system that is not justified. In addition, the laboratory ends up doing a lot of work that it cannot get reimbursed for. However, I would like to hear from those of you who have experience with it. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 135, Issue 1 **************************************** From mucram11 <@t> comcast.net Tue Feb 3 11:24:22 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Tue Feb 3 11:24:41 2015 Subject: [Histonet] ARKANSAS SPRING MEETING INCLUDES 1 DAY HT READINESS In-Reply-To: <2064296897.4706183.1422984226265.JavaMail.zimbra@comcast.net> Message-ID: <430097600.4706323.1422984262763.JavaMail.zimbra@comcast.net> Shane Jones will be presenting a one day class for HT Readiness for anyone who needs review for the exam this spring or early summer.? The cost will be $60.00 for the day and includes lunch and breaks.? Please let me know if you need further information about the class or if you would like to attend. ? Thank You, ? Pam Marcum ? From anna.m.rorick <@t> gmail.com Tue Feb 3 11:50:33 2015 From: anna.m.rorick <@t> gmail.com (Anna Rorick) Date: Tue Feb 3 11:50:41 2015 Subject: [Histonet] Problems with Universal Cassette Clamp Message-ID: <03441E3C-E699-4EB1-A647-5F47D2B67B23@gmail.com> Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone From bouchalr <@t> wvuhealthcare.com Tue Feb 3 12:18:18 2015 From: bouchalr <@t> wvuhealthcare.com (Bouchal, Rena L) Date: Tue Feb 3 12:18:39 2015 Subject: [Histonet] Problems with Universal Cassette Clamp In-Reply-To: <03441E3C-E699-4EB1-A647-5F47D2B67B23@gmail.com> References: <03441E3C-E699-4EB1-A647-5F47D2B67B23@gmail.com> Message-ID: <3F978C8467623541BA99D7EBFCF4BBD5F7FF7414@NT-EX2.wvuhs.com> I think we have had several break over the years... Is this where they say you have to buy the whole assembly for over $1000 or $1400 too?? We took a clamp off another older instrument. Rena Bouchal, M.S. Manager, Anatomic Pathology 304-293-7765 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Rorick Sent: Tuesday, February 03, 2015 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with Universal Cassette Clamp Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From srishan <@t> mail.holyname.org Tue Feb 3 12:20:09 2015 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Tue Feb 3 12:20:18 2015 Subject: [Histonet] time study on preparation of block and H& E slide Message-ID: Hi Histonetters, Has anyone has some information about time studies done of how long it takes to make a paraffin block and H&E slide. Thanks Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From Mary.Leslie <@t> tricore.org Tue Feb 3 12:20:59 2015 From: Mary.Leslie <@t> tricore.org (Leslie, Mary) Date: Tue Feb 3 12:21:06 2015 Subject: [Histonet] RE: Histonet Digest, Vol 135, Issue 2 In-Reply-To: References: Message-ID: Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for some great programs for lean management in a laboratory setting (both cytology and histology). Can anyone help me out? Thank you, LeAnn Hi Leann, I reached out to our primary vendors (Leica, Ventana and Sakura) to come in and do a lean assessment. These companies provide free lean services you can ask for. Mary J. Leslie, BSMT(ASCP),MS(CPM) Manager, Core Laboratories Operations Tricore Reference Laboratories Office: 505-938-8409 Cellphone: 505-264-5506 mary.leslie@tricore.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, February 03, 2015 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: "Know Error" (Teri Johnson) 2. QC for IHC bulk solutions (Davis, Cassie) 3. Benchmark Special Stainers (Bitting, Angela K.) 4. Re: Benchmark Special Stainers (STACEY) 5. RE: Benchmark Special Stainers (Bitting, Angela K.) 6. Lean Management (LeAnn Lang) 7. QIHC (Algeo, Lacie A) 8. RE: QIHC (Bea DeBrosse-Serra) 9. RE: Know Error (Cesar Francisco Romero) 10. Storage of paraffin blocks (Fawn Bomar) 11. RE: Storage of paraffin blocks (Rathborne, Toni) 12. RE: Know Error (Casey Arnold) 13. ARKANSAS SPRING MEETING INCLUDES 1 DAY HT READINESS (Pam Marcum) 14. Problems with Universal Cassette Clamp (Anna Rorick) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Feb 2015 18:13:33 +0000 From: Teri Johnson Subject: [Histonet] RE: "Know Error" To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" That sounds like a valid concern, Richard. Thank you for being an advocate for good utilization of patient testing. What is behind their concern? Do they think there has been a mistake with patient identification in your lab? Or are they trying to reconcile low PSA or other discordant screening test results with a diagnosis of prostate CA? I would offer to send whatever they need from the lab (paraffin section curls, unstained slides, etc) back to their office, so long as they provide patient consent. The urologist's office can then package that material with the buccal swabs they get from the patient. The onus can be on them to manage the cost of the testing, and they can then try to justify it with the patient when insurance will not reimburse for it. Is this option feasible? Teri ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Mon, 2 Feb 2015 13:24:02 -0500 From: "Davis, Cassie" Subject: [Histonet] QC for IHC bulk solutions To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good afternoon Histoland folks, other than documenting the ph of each Ventana bulk solution after diluting and visual inspections of slides run with that solution lot what other way is there to QC the EZ Prep, Reaction Buffer, and Liquid Coverslip bulks solutions? (we currently have a lively dialog going on about this) There is always something to do and something new in Histology... Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Mon, 2 Feb 2015 20:48:15 +0000 From: "Bitting, Angela K." Subject: [Histonet] Benchmark Special Stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7E3DB1@GHSEXMBX1W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" Can anyone tell me if the waste collection on the Benchmark Special Stainer is the same as the waste collection on the Benchmark Ultra or XT? Carboy with spigot?? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------ Message: 4 Date: Mon, 2 Feb 2015 12:56:49 -0800 From: STACEY Subject: Re: [Histonet] Benchmark Special Stainers To: "Angela K.Bitting" , "histonet@lists.utsouthwestern.edu" Message-ID: <1422910609.7337.YahooMailBasic@web181402.mail.ne1.yahoo.com> Content-Type: text/plain; charset=us-ascii Yes, it is the same. (Carboy with a spigot.) Stacey Merica, B.S., HT Histology Supervisor North Kansas City Hospital North Kansas City, MO 64116 ------------------ On Mon, 2/2/15, Bitting, Angela K. wrote: Subject: [Histonet] Benchmark Special Stainers To: "histonet@lists.utsouthwestern.edu" Date: Monday, February 2, 2015, 2:48 PM Can anyone tell me if the waste collection on the Benchmark Special Stainer is the same as the waste collection on the Benchmark Ultra or XT? Carboy with spigot?? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 2 Feb 2015 21:00:13 +0000 From: "Bitting, Angela K." Subject: RE: [Histonet] Benchmark Special Stainers To: STACEY , "histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7E3E0A@GHSEXMBX1W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" Thank you everyone for your swift replies. -----Original Message----- From: STACEY [mailto:staceyjm@sbcglobal.net] Sent: Monday, February 02, 2015 3:57 PM To: Bitting, Angela K.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Benchmark Special Stainers Yes, it is the same. (Carboy with a spigot.) Stacey Merica, B.S., HT Histology Supervisor North Kansas City Hospital North Kansas City, MO 64116 ------------------ On Mon, 2/2/15, Bitting, Angela K. wrote: Subject: [Histonet] Benchmark Special Stainers To: "histonet@lists.utsouthwestern.edu" Date: Monday, February 2, 2015, 2:48 PM Can anyone tell me if the waste collection on the Benchmark Special Stainer is the same as the waste collection on the Benchmark Ultra or XT? Carboy with spigot?? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 2 Feb 2015 21:13:19 +0000 From: LeAnn Lang Subject: [Histonet] Lean Management To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for some great programs for lean management in a laboratory setting (both cytology and histology). Can anyone help me out? Thank you, LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com ------------------------------ Message: 7 Date: Mon, 2 Feb 2015 22:13:48 +0000 From: "Algeo, Lacie A" Subject: [Histonet] QIHC To: "histonet@lists.utsouthwestern.edu" Message-ID: <24C4B3C167E5694887AB594C7602CE3A03B860B6@WN35104.or.providence.org> Content-Type: text/plain; charset="us-ascii" Hi All, What are the best methods for studying for the QIHC? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ------------------------------ Message: 8 Date: Mon, 2 Feb 2015 23:20:00 +0000 From: Bea DeBrosse-Serra Subject: [Histonet] RE: QIHC To: "Algeo, Lacie A" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Dako IHC booklet. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Monday, February 02, 2015 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hi All, What are the best methods for studying for the QIHC? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 3 Feb 2015 00:30:52 -0300 From: Cesar Francisco Romero Subject: [Histonet] RE: Know Error To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I live in a Corrupt Country . I can assure to you that this request is simply that, Corruption. The well done job is not well paid. The one that returns money to a corrupt person or a group of persons , yes, it is well paid. C?sar RomeroBuenos AiresArgentina ------------------------------ Message: 10 Date: Tue, 3 Feb 2015 13:48:20 +0000 From: Fawn Bomar Subject: [Histonet] Storage of paraffin blocks To: "histonet@lists.utsouthwestern.edu" Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B97E55F5@EXCH-2K10.hrhs.com> Content-Type: text/plain; charset="iso-8859-1" Hi everyone, I hope all is going well in the new year. I was wondering if everyone that stores their paraffin blocks in the plastic tissue tek block filing cabinets, stores these cabinets directly on the floor or do you all place them on some sort of stand? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 11 Date: Tue, 3 Feb 2015 13:58:02 +0000 From: "Rathborne, Toni" Subject: [Histonet] RE: Storage of paraffin blocks To: 'Fawn Bomar' , "histonet@lists.utsouthwestern.edu" Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4264A@vap1014.win.rwjuh.edu> Content-Type: text/plain; charset="us-ascii" To comply with Joint Commission standards, ours are stored 6" off the floor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Tuesday, February 03, 2015 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of paraffin blocks Hi everyone, I hope all is going well in the new year. I was wondering if everyone that stores their paraffin blocks in the plastic tissue tek block filing cabinets, stores these cabinets directly on the floor or do you all place them on some sort of stand? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 2 Feb 2015 18:37:30 +0000 From: Casey Arnold Subject: [Histonet] RE: Know Error To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have many clients who perform "Know Error" for their patients. What we do is send out only one site with malignancy (preferably the highest grade) instead of all sites. This cuts down on a lot of unnecessary work to be done by the tech and cuts costs for you. I find it easiest and most cost effective to send them the vial of tissue instead of the slides. I also believe that if you contact a Know Error representative you should be able to develop a contract to ensure reimbursement. Hope this helps! Casey Arnold Histology Supervisor 1450 Elm Hill Pike Nashville, TN? 37210 Office:? 615.874.0410 Cell:? 615.714.2940 Fax:? 615.345.4595 carnold@opko.com www.opko.com NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information.? Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, February 02, 2015 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH (Marcum, Pamela A) 2. "Know Error" (Cartun, Richard) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Feb 2015 14:22:58 +0000 From: "Marcum, Pamela A" Subject: [Histonet] ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH To: "histonet@lists.utsouthwestern.edu" Message-ID: <56d5892a005f4deaaf2cfed127d975b9@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Good Morning All, THE ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH, 2015 IN LITTLE ROCK, ARKANSAS PLEASE JOU US!! The meeting is being held at the Baptist Schools of Health in Little Rock and is open to anyone in Histology and related or interested fields. The following list is for the speakers, who will all be presenting 90 minute seminars for the meeting. We will have two rooms of speakers so you have a choice in classes and topics for the day. 7:30AM Registration 8AM to 9:30AM Seminars Dr Ericka Olgaard from UAMS Diagnostic Challenges of Breast Cancer Dr Daisy Alapat UAMS Methods and Basics of Protein Detection in Hematopathology 9:30AM to 10:00AM BREAK 10:00AM to 11:30AM Seminars Dr Shree Sharma Nephropath Integrating Mobile Technology With Anatomic Pathology Debbie Siena Technical Specialist Statlab Controlling Your Special Stains 11:30AM to 1:00PM Lunch 1:00PM to 2:30PM Seminars Dr Sara Shalin UAMS How and Why Skin Is Different Jennifer Feldman Ventana Antibody Production 2:30PM to 3:00PM Break 3:00PM to 4:30PM Seminars Dr Jennifer Forsythe ME Little Rock Autopsy in America Pamela Marcum HT UAMS Reagent Alcohol - Can't Drink It - What Is It? Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Mon, 2 Feb 2015 16:52:40 +0000 From: "Cartun, Richard" Subject: [Histonet] "Know Error" To: "histonet@lists.utsouthwestern.edu" Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3E0E26AE@HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Our laboratory has a new urology group who is demanding that we send-out every "Positive" (with CA) prostate biopsy for the "Know Error" DNA identity test. The test is used to confirm the identity of the patient diagnosed with cancer by matching the DNA in the cancer cells to that obtained from a buccal swab from the patient obtained in the doctor's office. In my opinion, this test is absolutely unnecessary; another expense ($1,700 list price) to our healthcare system that is not justified. In addition, the laboratory ends up doing a lot of work that it cannot get reimbursed for. However, I would like to hear from those of you who have experience with it. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 135, Issue 1 **************************************** ------------------------------ Message: 13 Date: Tue, 3 Feb 2015 17:24:22 +0000 (UTC) From: Pam Marcum Subject: [Histonet] ARKANSAS SPRING MEETING INCLUDES 1 DAY HT READINESS To: Histonet Message-ID: <430097600.4706323.1422984262763.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 Shane Jones will be presenting a one day class for HT Readiness for anyone who needs review for the exam this spring or early summer.?? The cost will be $60.00 for the day and includes lunch and breaks.?? Please let me know if you need further information about the class or if you would like to attend. ?? Thank You, ?? Pam Marcum ?? ------------------------------ Message: 14 Date: Tue, 3 Feb 2015 12:50:33 -0500 From: Anna Rorick Subject: [Histonet] Problems with Universal Cassette Clamp To: "histonet@lists.utsouthwestern.edu" Message-ID: <03441E3C-E699-4EB1-A647-5F47D2B67B23@gmail.com> Content-Type: text/plain; charset=us-ascii Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 135, Issue 2 **************************************** From PAMarcum <@t> uams.edu Tue Feb 3 12:34:19 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Feb 3 12:34:29 2015 Subject: [Histonet] Problems with Universal Cassette Clamp In-Reply-To: <03441E3C-E699-4EB1-A647-5F47D2B67B23@gmail.com> References: <03441E3C-E699-4EB1-A647-5F47D2B67B23@gmail.com> Message-ID: <0db96e4672fb4dcca87fd695e8a51146@MAIL13M2N2.ad.uams.edu> I have been using RM2255 for almost 10 years and never had a clamp problem. We have six in the lab with three of them more than 8 years old and other than routine PMs or the occasional human err problem these are great workhorses. We keep contracts on them so we have great service. I used one for about 4 years and cut plastic on it. The clamp was universal just not the same as the one on paraffin microtomes. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Rorick Sent: Tuesday, February 03, 2015 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with Universal Cassette Clamp Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From CThornton <@t> dahlchase.com Tue Feb 3 12:59:04 2015 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue Feb 3 12:59:13 2015 Subject: [Histonet] RE: Know Error Message-ID: This may sound like a silly question, Rich, (or maybe not), but how do you know the buccal swab is properly labeled? My experience is that when there's a mix-up in patients' tissue, it often occurs in the doctor's office. A lot of pathology labs are barcoding, but all the tracking in the world can't prevent a mix up at the doctor's office. How are they ensuring that their DNA swabs haven't gotten switched? Sounds like frivolous spending to me...and where is the line drawn? When are we sending out DNA testing on all specimens, not just prostate biopsies? Good luck! Clare Clare J. Thornton, HTL(ASCP),QIHC Lead Immunohistochemistry Tech Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, February 03, 2015 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://cp.mcafee.com/d/5fHCNASyMCCqenD7PhOedTdETpo7fKcKfCQrII3DT66rCQrIecKc9T76QrII3xOqab9KQOtoMDm0agQCvoDJfVsSxFc-NfqvOVJRx2Wa33_nVUsCUPRXBQQQQ-h7nppoVqWdAklrEEYG7DR8OJMddECPhOrKrKr01DOFeDdPFlKpcNZ9_67uwTwCHIcfBisEeRMDaAWwQSjBitgqn8lrxrW0E-l9QVKtaJP9CfFfUMXQ6PqpEVsvKr2YGjG3jh07i8_io1Cy0cRfd42bbU1Cy0dFhLPh0CrppdWvc9jYdQ or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: "Know Error" (Teri Johnson) 2. QC for IHC bulk solutions (Davis, Cassie) 3. Benchmark Special Stainers (Bitting, Angela K.) 4. Re: Benchmark Special Stainers (STACEY) 5. RE: Benchmark Special Stainers (Bitting, Angela K.) 6. Lean Management (LeAnn Lang) 7. QIHC (Algeo, Lacie A) 8. RE: QIHC (Bea DeBrosse-Serra) 9. RE: Know Error (Cesar Francisco Romero) 10. Storage of paraffin blocks (Fawn Bomar) 11. RE: Storage of paraffin blocks (Rathborne, Toni) 12. RE: Know Error (Casey Arnold) 13. ARKANSAS SPRING MEETING INCLUDES 1 DAY HT READINESS (Pam Marcum) 14. Problems with Universal Cassette Clamp (Anna Rorick) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Feb 2015 18:13:33 +0000 From: Teri Johnson Subject: [Histonet] RE: "Know Error" To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" That sounds like a valid concern, Richard. Thank you for being an advocate for good utilization of patient testing. What is behind their concern? Do they think there has been a mistake with patient identification in your lab? Or are they trying to reconcile low PSA or other discordant screening test results with a diagnosis of prostate CA? I would offer to send whatever they need from the lab (paraffin section curls, unstained slides, etc) back to their office, so long as they provide patient consent. The urologist's office can then package that material with the buccal swabs they get from the patient. The onus can be on them to manage the cost of the testing, and they can then try to justify it with the patient when insurance will not reimburse for it. Is this option feasible? Teri ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Mon, 2 Feb 2015 13:24:02 -0500 From: "Davis, Cassie" Subject: [Histonet] QC for IHC bulk solutions To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good afternoon Histoland folks, other than documenting the ph of each Ventana bulk solution after diluting and visual inspections of slides run with that solution lot what other way is there to QC the EZ Prep, Reaction Buffer, and Liquid Coverslip bulks solutions? (we currently have a lively dialog going on about this) There is always something to do and something new in Histology... Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Mon, 2 Feb 2015 20:48:15 +0000 From: "Bitting, Angela K." Subject: [Histonet] Benchmark Special Stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7E3DB1@GHSEXMBX1W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" Can anyone tell me if the waste collection on the Benchmark Special Stainer is the same as the waste collection on the Benchmark Ultra or XT? Carboy with spigot?? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------ Message: 4 Date: Mon, 2 Feb 2015 12:56:49 -0800 From: STACEY Subject: Re: [Histonet] Benchmark Special Stainers To: "Angela K.Bitting" , "histonet@lists.utsouthwestern.edu" Message-ID: <1422910609.7337.YahooMailBasic@web181402.mail.ne1.yahoo.com> Content-Type: text/plain; charset=us-ascii Yes, it is the same. (Carboy with a spigot.) Stacey Merica, B.S., HT Histology Supervisor North Kansas City Hospital North Kansas City, MO 64116 ------------------ On Mon, 2/2/15, Bitting, Angela K. wrote: Subject: [Histonet] Benchmark Special Stainers To: "histonet@lists.utsouthwestern.edu" Date: Monday, February 2, 2015, 2:48 PM Can anyone tell me if the waste collection on the Benchmark Special Stainer is the same as the waste collection on the Benchmark Ultra or XT? Carboy with spigot?? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/avndyhJ5xdcQsLefCzAsrKrhKOMevspsvdETpo7fKccTdETospsojKedETpo73AQkmjtFAWNxeI0kxFc-NfqvOVJ3ipZyuQ_BPrH25Qk67-LPMVdNDHTbFFFFYyeKOONORQr8EGThhVkffGhBrwqrjdCzATsTsS03fBiterDiHsOpzWj-ceZ1L1dnoovaAVgtHxel9R1FIDaAWwQKgGT2TQ1hYGjFPsWlrCjcvivNxTEdCQPhOU_sS5VkDk6Cy0eAh-AM3d40pGuq84mnM3d40rizvCy1cSOOrbsp0zb7j ------------------------------ Message: 5 Date: Mon, 2 Feb 2015 21:00:13 +0000 From: "Bitting, Angela K." Subject: RE: [Histonet] Benchmark Special Stainers To: STACEY , "histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7E3E0A@GHSEXMBX1W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" Thank you everyone for your swift replies. -----Original Message----- From: STACEY [mailto:staceyjm@sbcglobal.net] Sent: Monday, February 02, 2015 3:57 PM To: Bitting, Angela K.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Benchmark Special Stainers Yes, it is the same. (Carboy with a spigot.) Stacey Merica, B.S., HT Histology Supervisor North Kansas City Hospital North Kansas City, MO 64116 ------------------ On Mon, 2/2/15, Bitting, Angela K. wrote: Subject: [Histonet] Benchmark Special Stainers To: "histonet@lists.utsouthwestern.edu" Date: Monday, February 2, 2015, 2:48 PM Can anyone tell me if the waste collection on the Benchmark Special Stainer is the same as the waste collection on the Benchmark Ultra or XT? Carboy with spigot?? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/avndygQ86Qm4QPhOYU-qehNKVJ6Xb0VZNBNYSztBws-UMPsSztxNBNxeUUSztBwsejhhpdSCjH64WM1i6APX4ZF_bCQd9DS9Xj-ndKI8nhgovW_f3AT6uLsKCCCDO8WXbb7bnhIyyHt57BgY-F6lK1FJASqejtPtPo0c-l9QVKtaJP9CfFfUMXQ6Y4RtxxYGjB1SK4VkDk6COsGjG3iV2Hsbvg57OFeDdPFlKpcNZ9_67uwSrjd7bzZPonBitgqq80Wh7Wj0cQg1CFVEwhpv0cQg1Jad-q84Prb9LSYnqvc9 ------------------------------ Message: 6 Date: Mon, 2 Feb 2015 21:13:19 +0000 From: LeAnn Lang Subject: [Histonet] Lean Management To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for some great programs for lean management in a laboratory setting (both cytology and histology). Can anyone help me out? Thank you, LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com ------------------------------ Message: 7 Date: Mon, 2 Feb 2015 22:13:48 +0000 From: "Algeo, Lacie A" Subject: [Histonet] QIHC To: "histonet@lists.utsouthwestern.edu" Message-ID: <24C4B3C167E5694887AB594C7602CE3A03B860B6@WN35104.or.providence.org> Content-Type: text/plain; charset="us-ascii" Hi All, What are the best methods for studying for the QIHC? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ------------------------------ Message: 8 Date: Mon, 2 Feb 2015 23:20:00 +0000 From: Bea DeBrosse-Serra Subject: [Histonet] RE: QIHC To: "Algeo, Lacie A" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Dako IHC booklet. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Monday, February 02, 2015 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC Hi All, What are the best methods for studying for the QIHC? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/1jWVIq6xEgdEI9FCzBVNYQszztPqdSm1PXzbzVJ6Xb0VZNxCVJ6X3zbz2tNNJ6Xb0UsCyyOrJcDmc9Rw2Ad9DS9Xj-ndEqjfIjSDYKrtogKywM_R-u79KcZuVtdddfAhRSmmemKzp55mWafaxVZicHs3jr1IQsCXCXCM0pYGjFPsWlrCjcvivNxTEdU9GX33VkDa3Js9OFeEddAVkDk6BO5mUm-wafBiterDiHsOpzWj-ceZ1ISCqen7XCMLaAWwQQg1QyfQC0pEw3djPh0yO-0pEw3qkrYQg9CSmjqt5BD0tv ------------------------------ Message: 9 Date: Tue, 3 Feb 2015 00:30:52 -0300 From: Cesar Francisco Romero Subject: [Histonet] RE: Know Error To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I live in a Corrupt Country . I can assure to you that this request is simply that, Corruption. The well done job is not well paid. The one that returns money to a corrupt person or a group of persons , yes, it is well paid. C?sar RomeroBuenos AiresArgentina ------------------------------ Message: 10 Date: Tue, 3 Feb 2015 13:48:20 +0000 From: Fawn Bomar Subject: [Histonet] Storage of paraffin blocks To: "histonet@lists.utsouthwestern.edu" Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B97E55F5@EXCH-2K10.hrhs.com> Content-Type: text/plain; charset="iso-8859-1" Hi everyone, I hope all is going well in the new year. I was wondering if everyone that stores their paraffin blocks in the plastic tissue tek block filing cabinets, stores these cabinets directly on the floor or do you all place them on some sort of stand? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 11 Date: Tue, 3 Feb 2015 13:58:02 +0000 From: "Rathborne, Toni" Subject: [Histonet] RE: Storage of paraffin blocks To: 'Fawn Bomar' , "histonet@lists.utsouthwestern.edu" Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4264A@vap1014.win.rwjuh.edu> Content-Type: text/plain; charset="us-ascii" To comply with Joint Commission standards, ours are stored 6" off the floor. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Tuesday, February 03, 2015 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of paraffin blocks Hi everyone, I hope all is going well in the new year. I was wondering if everyone that stores their paraffin blocks in the plastic tissue tek block filing cabinets, stores these cabinets directly on the floor or do you all place them on some sort of stand? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/1jWVIg4x0SyMCCqenD7PhOedTdETpo7fKcKfCQrII3DT66rCQrIecKc9T76QrII3xOqab9KQOtoMDm0agQCvoDJfVsSxFc-NfqvOVJRx2Wa33_nVUsCUPRXBQQQQ-h7nppoVqWdAklrEEYG7DR8OJMddLCPhOrKrKr01DOFeDdPFlKpcNZ9_67uwTwCHIcfBisEeRMDaAWwQSjBitgqn8lrxrW0E-l9QVKtaJP9CfFfUMXQ6PqpEVsvKr2YGjG3jh07i8_io1Cy0cRfd42bbU1Cy0dFhLPh0CrppdRlQGaVTX ------------------------------ Message: 12 Date: Mon, 2 Feb 2015 18:37:30 +0000 From: Casey Arnold Subject: [Histonet] RE: Know Error To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We have many clients who perform "Know Error" for their patients. What we do is send out only one site with malignancy (preferably the highest grade) instead of all sites. This cuts down on a lot of unnecessary work to be done by the tech and cuts costs for you. I find it easiest and most cost effective to send them the vial of tissue instead of the slides. I also believe that if you contact a Know Error representative you should be able to develop a contract to ensure reimbursement. Hope this helps! Casey Arnold Histology Supervisor 1450 Elm Hill Pike Nashville, TN? 37210 Office:? 615.874.0410 Cell:? 615.714.2940 Fax:? 615.345.4595 carnold@opko.com http://cp.mcafee.com/d/k-Kr6x0q6zqb2qpEVusvd78UTsSztBws-UOU-rhKOMevsopKrhKMUOUMDssrhKOMe79EEICXj9Rz2to0F3ipZyuQ_BPq6APX4ZF_bCTm4bEEcfZvDxOrzfnKnjjjjV4ttBBzBHEShhlKyzOEuvkzaT0QSUrd79KVKVIFRlyJundAVkDk6BO5mUm-wafBiterDiHsOpzWj-ceZ1ISCqen7XCMLaAWwQQg1QyfQC0pEw3djPh0yO-0pEw3qkrYQg9CSmju1_vjPCjD8VX NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information.? Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, February 02, 2015 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://cp.mcafee.com/d/5fHCN0g6xASyMCCqenD7PhOedTdETpo7fKcKfCQrII3DT66rCQrIecKc9T76QrII3xOqab9KQOtoMDm0agQCvoDJfVsSxFc-NfqvOVJRx2Wa33_nVUsCUPRXBQQQQ-h7nppoVqWdAklrEEYG7DR8OJMddzzpEVdTdTdw0PVkDjCVQGTcCo-A_z3LgrMjlS67OFek7qUjBitgqr9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJcQsKfTdxul9R1FEw3F4vFc0Ph06qDCy15BY0Ph06QETVEwjdIICMhppUfFL or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH (Marcum, Pamela A) 2. "Know Error" (Cartun, Richard) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Feb 2015 14:22:58 +0000 From: "Marcum, Pamela A" Subject: [Histonet] ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH To: "histonet@lists.utsouthwestern.edu" Message-ID: <56d5892a005f4deaaf2cfed127d975b9@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Good Morning All, THE ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH, 2015 IN LITTLE ROCK, ARKANSAS PLEASE JOU US!! The meeting is being held at the Baptist Schools of Health in Little Rock and is open to anyone in Histology and related or interested fields. The following list is for the speakers, who will all be presenting 90 minute seminars for the meeting. We will have two rooms of speakers so you have a choice in classes and topics for the day. 7:30AM Registration 8AM to 9:30AM Seminars Dr Ericka Olgaard from UAMS Diagnostic Challenges of Breast Cancer Dr Daisy Alapat UAMS Methods and Basics of Protein Detection in Hematopathology 9:30AM to 10:00AM BREAK 10:00AM to 11:30AM Seminars Dr Shree Sharma Nephropath Integrating Mobile Technology With Anatomic Pathology Debbie Siena Technical Specialist Statlab Controlling Your Special Stains 11:30AM to 1:00PM Lunch 1:00PM to 2:30PM Seminars Dr Sara Shalin UAMS How and Why Skin Is Different Jennifer Feldman Ventana Antibody Production 2:30PM to 3:00PM Break 3:00PM to 4:30PM Seminars Dr Jennifer Forsythe ME Little Rock Autopsy in America Pamela Marcum HT UAMS Reagent Alcohol - Can't Drink It - What Is It? Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Mon, 2 Feb 2015 16:52:40 +0000 From: "Cartun, Richard" Subject: [Histonet] "Know Error" To: "histonet@lists.utsouthwestern.edu" Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3E0E26AE@HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Our laboratory has a new urology group who is demanding that we send-out every "Positive" (with CA) prostate biopsy for the "Know Error" DNA identity test. The test is used to confirm the identity of the patient diagnosed with cancer by matching the DNA in the cancer cells to that obtained from a buccal swab from the patient obtained in the doctor's office. In my opinion, this test is absolutely unnecessary; another expense ($1,700 list price) to our healthcare system that is not justified. In addition, the laboratory ends up doing a lot of work that it cannot get reimbursed for. However, I would like to hear from those of you who have experience with it. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/avndz8wrhojjd7bPzVEV76XCQrII3DT6n7PqdSm1PXz3dPqdS76n64XzzqdSm1MVd55ATqpeIojH058qjfIjSDYKrgQCvoDJfVsSWMxt51x_HYYejspWZOWqqqv8zHIIIsJt6OaaJQkul3PWApmU6CTzpEVdTdTdw0PVkDjCVQGTcCo-A_z3LgrMjlS67OFek7qUjBitgqr9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJcQsKfTdxul9R1FEw3F4vFc0Ph06qDCy15BY0Ph06QETVEwjdIICNpywegOM End of Histonet Digest, Vol 135, Issue 1 **************************************** ------------------------------ Message: 13 Date: Tue, 3 Feb 2015 17:24:22 +0000 (UTC) From: Pam Marcum Subject: [Histonet] ARKANSAS SPRING MEETING INCLUDES 1 DAY HT READINESS To: Histonet Message-ID: <430097600.4706323.1422984262763.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 Shane Jones will be presenting a one day class for HT Readiness for anyone who needs review for the exam this spring or early summer.?? The cost will be $60.00 for the day and includes lunch and breaks.?? Please let me know if you need further information about the class or if you would like to attend. ?? Thank You, ?? Pam Marcum ?? ------------------------------ Message: 14 Date: Tue, 3 Feb 2015 12:50:33 -0500 From: Anna Rorick Subject: [Histonet] Problems with Universal Cassette Clamp To: "histonet@lists.utsouthwestern.edu" Message-ID: <03441E3C-E699-4EB1-A647-5F47D2B67B23@gmail.com> Content-Type: text/plain; charset=us-ascii Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/2DRPowcygArhojjd7bPzVEV76XCQrII3DT6n7PqdSm1PXz3dPqdS76n64XzzqdSm1MVd55ATqpeIojH058qjfIjSDYKrgQCvoDJfVsSWMxt51x_HYYejspWZOWqqqv8zHIIIsJt6OaaJQkul3PWApmU6CPhOrd79KVKVI06vaAWsTeBmVAP7QDYotW3u2qKMM-l9OwXn2sGjG3jpel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdFCzBN-VIbOFeEdd40t8zZ9w6q80PkYQg8ILw6q80SB6_d42pJBASyYzm2MO-u2e End of Histonet Digest, Vol 135, Issue 2 **************************************** From Pamela.S.Younes <@t> uth.tmc.edu Tue Feb 3 13:19:39 2015 From: Pamela.S.Younes <@t> uth.tmc.edu (Younes, Pamela S) Date: Tue Feb 3 13:19:48 2015 Subject: [Histonet] ThermoShandon Gemini AS Stainer w/ heat Message-ID: Hello everyone, My director has asked if I can find the average lifespan of this stainer, and your experience. We are trying to justify a new stainer. Many thanks, Pam Pamela S. Younes MHS, HTL(ASCP), CPC, PA(ASCP) Assistant Professor UT Medical School Houston, Department of Pathology and Laboratory Medicine 6431 Fannin, MSB 2.136, Houston, TX 77030 (713) 566-5046 Pamela.S.Younes@uth.tmc.edu From HMLaudon <@t> gundersenhealth.org Tue Feb 3 13:19:42 2015 From: HMLaudon <@t> gundersenhealth.org (Laudon, Heather M) Date: Tue Feb 3 13:20:48 2015 Subject: [Histonet] C1q FITC In-Reply-To: References: Message-ID: Does anyone have a vendor with C1q FITC availability? We typically order Dako product #F0254 which is a polyclonal rabbit anti-human C1q complement, but are told they are on backorder for an undetermined amount of time (possibly May 2015). Thanks for your help! Heather Laudon, BS, HTL (ASCP)cm Histology Technician Surgical Pathology Laboratory Gundersen Health System 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 From JRobinson <@t> pathology-associates.com Tue Feb 3 13:27:00 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Tue Feb 3 13:27:23 2015 Subject: [Histonet] Problems with Universal Cassette Clamp In-Reply-To: <03441E3C-E699-4EB1-A647-5F47D2B67B23@gmail.com> References: <03441E3C-E699-4EB1-A647-5F47D2B67B23@gmail.com> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8BC65@PAEXCH1.PathologyAssociates.local> Yes, unfortunately I am very familiar with this problem. We have probably had at least 10 clamp handles break off over the last 3 years. The problem is the soft metal they are using for the clamp post. The post fits into a hole in the bottom portion of the clamp and that is actually what pulls the clamp open so that you can insert a cassette. I have talked to our Leica rep numerous times about this issue. They first tried to blame our techs for the problem (they must be using it too "aggressively") but they eventually took a limited amount of "ownership" for the inferior materials. At one point they thickened the clamp handle itself but the real problem is that post at the end of the handle. I have suggested that they make the handle (or at least the post) out of titanium or some other material that can stand up to the stress put on it by the clamp. I have not seen any additional improvements over the past two years and we still have clamp handles break off on occasion. Your Leica rep is well aware of this problem so contact them and ask for some new clamps (they will hopefully offer a free one or discounted one). I try to keep a couple of new spare clamps on hand as I have yet to figure out a way to repair that handle once it breaks off. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Rorick Sent: Tuesday, February 03, 2015 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with Universal Cassette Clamp Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From wbenton <@t> cua.md Tue Feb 3 13:29:23 2015 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Feb 3 13:29:30 2015 Subject: [Histonet] RE: C1q FITC In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD942394391FD@CUAEXH1.GCU-MD.local> http://www.ventana.com/product/180?type=174 http://www.thermoscientific.com/content/tfs/en/product/c1q-complement-fitc-labeled-antibody.html http://www.abcam.com/c1q-antibody-fitc-ab4223.html Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Tuesday, February 03, 2015 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C1q FITC Does anyone have a vendor with C1q FITC availability? We typically order Dako product #F0254 which is a polyclonal rabbit anti-human C1q complement, but are told they are on backorder for an undetermined amount of time (possibly May 2015). Thanks for your help! Heather Laudon, BS, HTL (ASCP)cm Histology Technician Surgical Pathology Laboratory Gundersen Health System 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From twebster <@t> CRH.org Tue Feb 3 13:52:13 2015 From: twebster <@t> CRH.org (Webster, Thomas S.) Date: Tue Feb 3 13:51:59 2015 Subject: [Histonet] RE: Know Error Message-ID: <7207186ED68FB542803CAF1CE6E82FF80873C51E@exmb2.crh.org> Most problems are with the physician offices, not the lab, because they have bad practices like pre-labeling specimens. How does this fix that problem? You could have a buccal specimen and biopsy that match up fine but are on the wrong patient. Seems like collecting a buccal smear just adds even more variables and opportunities for error. Spend that time properly labeling the ACTUAL specimen(s) and problem solved. There is at least one lab that is offering Know Error testing on abnormal pap tests believe it or not. I didn't realize an abnormal pap test lead immediately to a hysterectomy...... http://manhattanlabs.com/for-doctors/mypap/ CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From Donna.Ingersoll <@t> 21co.com Tue Feb 3 14:18:32 2015 From: Donna.Ingersoll <@t> 21co.com (Ingersoll, Donna S.) Date: Tue Feb 3 14:18:37 2015 Subject: [Histonet] RE: Know Error In-Reply-To: <7207186ED68FB542803CAF1CE6E82FF80873C51E@exmb2.crh.org> References: <7207186ED68FB542803CAF1CE6E82FF80873C51E@exmb2.crh.org> Message-ID: <2E6AB167B485B74DA65B52A040718DDF0D5FEF8CB0@DCO-MXS-MAIL00.rta.com> This sounds like marketing scheme directly to the clinicians and expecting the lab to assume the reponsiblity for the costs. Confidentiality Statement: This email and any files transmitted with it may contain confidential and/or privileged material and is intended only for the person or entity to which it is addressed. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you have received this email in error, please notify the sender immediately and delete this material from all known records. Thank you Confidentiality Statement: This email and any files transmitted with it may contain confidential and/or privileged material and is intended only for the person or entity to which it is addressed. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you have received this email in error, please notify the sender immediately and delete this material from all known records. Thank you -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Tuesday, February 03, 2015 2:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Know Error Most problems are with the physician offices, not the lab, because they have bad practices like pre-labeling specimens. How does this fix that problem? You could have a buccal specimen and biopsy that match up fine but are on the wrong patient. Seems like collecting a buccal smear just adds even more variables and opportunities for error. Spend that time properly labeling the ACTUAL specimen(s) and problem solved. There is at least one lab that is offering Know Error testing on abnormal pap tests believe it or not. I didn't realize an abnormal pap test lead immediately to a hysterectomy...... http://manhattanlabs.com/for-doctors/mypap/ CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Tue Feb 3 14:19:36 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Feb 3 14:19:43 2015 Subject: [Histonet] RE: Know Error In-Reply-To: <7207186ED68FB542803CAF1CE6E82FF80873C51E@exmb2.crh.org> References: <7207186ED68FB542803CAF1CE6E82FF80873C51E@exmb2.crh.org> Message-ID: <49846165.13120222.1422994776458.JavaMail.zimbra@comcast.net> I?am not disagreeing nor am I sticking up for the company and not sure I'd even agree with the company?but I think there is much more to this, from what little I know of them, than just mixing up two specimens at a physicians office.? I believe their point of view of the company is besides patient ID errors that in making a cancer diagnosis, that the cancer material came EXCLUSIVELY from that one patient and is not a contaminant from another real cancer patient.? Think floaters upon cutting, think leftover friable material from an embedding well that ends up in a cassette because embedder didn't clean forceps.? You PCR up a V600E BRAF mutation from a melanoma slide and tube and how do you know 100% it is not DNA from a different patient who really needs the therapeutic but the first patient doesn't.? Is the mutation really representative of the first patient or just a contaminant from the second?? Again, I truly wonder about this being useful but you have to admit, there is more than only patient mis-identification but there are floaters and contaminations that must be paid attention to.? Just my opinion. Ray Seattle, WA ----- Original Message ----- From: "Thomas S. Webster" To: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, February 3, 2015 11:52:13 AM Subject: [Histonet] RE: Know Error Most problems are with the physician offices, not the lab, because they have bad practices like pre-labeling specimens. How does this fix that problem? You could have a buccal specimen and biopsy that match up fine but are on the wrong patient. Seems like collecting a buccal smear just adds even more variables and opportunities for error. Spend that time properly labeling the ACTUAL specimen(s) and problem solved. There is at least one lab that is offering Know Error testing on abnormal pap tests believe it or not. I didn't realize an abnormal pap test lead immediately to a hysterectomy...... http://manhattanlabs.com/for-doctors/mypap/ CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. ?If you are not the intended recipient, please contact the sender by reply e-mail immediately. ?Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Tue Feb 3 15:01:45 2015 From: DSiena <@t> statlab.com (Debra Siena) Date: Tue Feb 3 15:02:21 2015 Subject: [Histonet] best practices for HIER in electric pressure cookers Message-ID: Hi Histonetters, I am doing some research into HIER in electric pressure cookers, are there any known CAP/CLIA regs about the cooker itself? Also what is the consensus on best practices for using electric pressure cooker these are my questions: should the amount of slides be standardized per run? should the amount of water each run be standardized how about the amount of containers each run? Temperature strips required each run? How about releasing the steam early vs letting pressure subside by itself prior to opening? Should HIER times be optimized as well as different pH solutions, for example should I test at different time intervals per retrieval solution for best results? Sorry for all the questions but it has been awhile since I have done manual retrieval so just want to find out what the guru's are saying. Thanks in advance, if you wish to reply off line that is good and I will publish the final results to the histonet. Thanks again Debbie Siena 800.442.3573 ext. 229 | www.statlab.com From PREISZNE <@t> mail.etsu.edu Tue Feb 3 15:20:13 2015 From: PREISZNE <@t> mail.etsu.edu (Preiszner, Johanna) Date: Tue Feb 3 15:20:25 2015 Subject: [Histonet] peach stone (pit) sectioning? Message-ID: Hi Netters, does anybody know how I could soften up a hard fruit pit for sectioning? Thanks, Hanna Preiszner ETSU/QCOM From hans <@t> histologistics.com Tue Feb 3 16:44:18 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Tue Feb 3 16:44:31 2015 Subject: [Histonet] peach stone (pit) sectioning? In-Reply-To: References: Message-ID: <4145B684-66A2-4B64-9857-9CF78398DC7C@histologistics.com> Hello, I have cut small pieces of pine (wood) on the cryostat by soaking it in warm water. I had to collect the sections in a water bath so they would unfold. The longer you soak it, especially warm or hit water, the softer it should in theory get. Good luck! Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com > On Feb 3, 2015, at 16:20, Preiszner, Johanna wrote: > > Hi Netters, > > does anybody know how I could soften up a hard fruit pit for sectioning? > > Thanks, > Hanna Preiszner > ETSU/QCOM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Feb 3 18:32:10 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Feb 3 18:32:37 2015 Subject: [Histonet] FW: Iron control tissue or blocks In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A464DB0062@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A3EAD3AEC5@EXCHANGE2K7.staprimecare.org> <1E0E2B14C709174B8AC2BE0AE7F76833A464DB0062@EXCHANGE2K7.staprimecare.org> Message-ID: Spleen is a good, sensitive control to use for iron. From: "Montana, Maria" To: "histonet@lists.utsouthwestern.edu" Date: 01/20/2015 08:39 AM Subject: [Histonet] FW: Iron control tissue or blocks Sent by: histonet-bounces@lists.utsouthwestern.edu ________________________________ From: Montana, Maria Sent: Wednesday, January 07, 2015 1:50 PM To: hisonet@lists.utsouthwestern.edu Subject: Iron control tissue or blocks Hi, Histoneters, I was wondering if anyone out there has some good Fe control tissue or blocks that you would be willing to trade for. We have Amyloid tissue, if you are interested. Thanks, Maria Montana HTL (ASCP) CHI St Alexius Health (701)530-6732 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From udsd007 <@t> gmail.com Tue Feb 3 21:16:01 2015 From: udsd007 <@t> gmail.com (Mike Andrews) Date: Tue Feb 3 21:16:12 2015 Subject: [Histonet] peach stone (pit) sectioning? In-Reply-To: References: Message-ID: <40176BFA-9833-4430-B516-07E42FFE5542@gmail.com> If you are just sectioning the (relatively softer) seed, then you might consider softening it in 4% EDT ethylene diamine, a.k.a. 1,2-diaminoethane. That _might_ work for the rather harder shell surrounding the seed, if you need to section that. Steaming the shell, or boiling it, night be necessary. You might find it worthwhile to consult _A Guide to Wood Microtomy_ by Ernie Ives (self-published, ISBN 0-9540551-0-1). A bit more information on your objective here would be helpful. Mike Andrews, W5EGO WWME Oklahoma area executive team > On Feb 3, 2015, at 3:20 PM, Preiszner, Johanna wrote: > > Hi Netters, > > does anybody know how I could soften up a hard fruit pit for sectioning? > > Thanks, > Hanna Preiszner > ETSU/QCOM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Wed Feb 4 08:18:22 2015 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Feb 4 08:18:59 2015 Subject: [Histonet] RE: "Know Error" In-Reply-To: <20150203165828.E1CCE1EA97B@trendmess-svr.holyredeemer.local> References: <20150203165828.E1CCE1EA97B@trendmess-svr.holyredeemer.local> Message-ID: Great advice, Teri! While I've not had the same request for the DNA identity test, I've had a similar circumstance with a request for "routine" testing of a specimen type that was beyond the scope of what our pathologists thought as necessary diagnostic or prognostic testing. Our response was, and is, exactly what Teri Johnson proposed. After discussing and addressing their concerns, we agreed that they would send us a signed patient release to send the block to their office for them to manage further testing, including any billing. It is a system that has worked well for us. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Feb 2015 18:13:33 +0000 From: Teri Johnson Subject: [Histonet] RE: "Know Error" That sounds like a valid concern, Richard. Thank you for being an advocate for good utilization of patient testing. What is behind their concern? Do they think there has been a mistake with patient identification in your lab? Or are they trying to reconcile low PSA or other discordant screening test results with a diagnosis of prostate CA? I would offer to send whatever they need from the lab (paraffin section curls, unstained slides, etc) back to their office, so long as they provide patient consent. The urologist's office can then package that material with the buccal swabs they get from the patient. The onus can be on them to manage the cost of the testing, and they can then try to justify it with the patient when insurance will not reimburse for it. Is this option feasible? Teri ------------------------------ Date: Mon, 2 Feb 2015 16:52:40 +0000 From: "Cartun, Richard" Subject: [Histonet] "Know Error" Our laboratory has a new urology group who is demanding that we send-out every "Positive" (with CA) prostate biopsy for the "Know Error" DNA identity test. The test is used to confirm the identity of the patient diagnosed with cancer by matching the DNA in the cancer cells to that obtained from a buccal swab from the patient obtained in the doctor's office. In my opinion, this test is absolutely unnecessary; another expense ($1,700 list price) to our healthcare system that is not justified. In addition, the laboratory ends up doing a lot of work that it cannot get reimbursed for. However, I would like to hear from those of you who have experience with it. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From rjr6 <@t> psu.edu Wed Feb 4 09:35:24 2015 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Wed Feb 4 09:35:39 2015 Subject: [Histonet] Shaker Message-ID: One of the pathologists here wants to but a shaker to put the containers with formalin and tissues on. Is this a feasible idea I've told them don't put the formalin tissues in the cooler and don't put the whole organ in the bottles Roberta Horner Animal Diagnostic Lab Penn State University From esarricks <@t> gmail.com Wed Feb 4 10:18:30 2015 From: esarricks <@t> gmail.com (Erin Sarricks) Date: Wed Feb 4 10:18:38 2015 Subject: [Histonet] VIP 3000 Processor Trays Message-ID: Hello Histonetters, Does anyone have a spare cassette processing tray (with or without the lid) for the VIP 2000/3000 that they would be willing to part with for a reasonable price- or know where I could get one for a reasonable price? We have searched around and most options we have found cost around $400, so I wanted to reach out to histonet to see if anyone had any other options. Thanks for your help and have a great day! Erin From aonomic <@t> auburn.edu Wed Feb 4 14:34:40 2015 From: aonomic <@t> auburn.edu (Michelle Aono) Date: Wed Feb 4 14:35:13 2015 Subject: [Histonet] Cloudy Superfrost Plus Slides Message-ID: <3A862E73E3BAEF4ABCA665F7E3AF0640999FCC5A@exmb2> Have some cloudy Fisher Superfrost plus slides. Sometimes they're cloudy even from boxes that are still in the plastic wrapping. Does the wisdom of the collective histonet think these slides are okay to use or should be tossed? Thanks! Michelle (Shelly) Aono ~~~~~~~~~~~~~~~~~~~ Research Associate II 107B/124 Greene Hall Auburn University, Dept of APP Auburn, AL 36849 (334) 844-5594 From PAMarcum <@t> uams.edu Wed Feb 4 15:00:37 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Feb 4 15:00:49 2015 Subject: [Histonet] RE: Cloudy Superfrost Plus Slides In-Reply-To: <3A862E73E3BAEF4ABCA665F7E3AF0640999FCC5A@exmb2> References: <3A862E73E3BAEF4ABCA665F7E3AF0640999FCC5A@exmb2> Message-ID: Have you checked lot numbers and expiration dates? I have only seen issues with slides that are stored in a very humid place or where the temperatures vary greatly over a year in storage. We use the ThermoFisher Colorfrost slides and have no problems here or where I lived in Pennsylvania. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Aono Sent: Wednesday, February 04, 2015 2:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cloudy Superfrost Plus Slides Have some cloudy Fisher Superfrost plus slides. Sometimes they're cloudy even from boxes that are still in the plastic wrapping. Does the wisdom of the collective histonet think these slides are okay to use or should be tossed? Thanks! Michelle (Shelly) Aono ~~~~~~~~~~~~~~~~~~~ Research Associate II 107B/124 Greene Hall Auburn University, Dept of APP Auburn, AL 36849 (334) 844-5594 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Steven.Swartwood <@t> cshs.org Wed Feb 4 15:16:55 2015 From: Steven.Swartwood <@t> cshs.org (Swartwood, Steven J) Date: Wed Feb 4 15:17:05 2015 Subject: [Histonet] Rat Neutrophils Message-ID: <959202AC61AEF942968646EC66E2BE3E690EEA9A@ESPWMSGMBX08.CSMC.EDU> Hello again everyone, I have a researcher requesting IHC staining for Rat Neutrophils. I've found a few papers on PubMed and a few antibodies that are specific for Rat neutrophils. The tissue being used is FFPE Rat pancreas and lungs. We are specifically looking for Rat neutrophils. There was no human neutrophils injected into the Rats. I just wanted to see if anyone out there does this or has done this recently and what antibodies they used. A protocol would be greatly appreciated as well. So far I've found more anti-MPO antibodies, but only 1 of them has actual pictures of neutrophil staining in Rats. I was looking into Ly6G antibodies, but none really looked too spectacular. Any and all information is greatly appreciated. I thank all you for your time/efforts for helping me now and the previous help I've received. Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartwood@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. From Joanna.Bartczak <@t> cls.ab.ca Wed Feb 4 15:24:47 2015 From: Joanna.Bartczak <@t> cls.ab.ca (Joanna Bartczak) Date: Wed Feb 4 15:24:53 2015 Subject: [Histonet] use of denatured alcohol In-Reply-To: References: Message-ID: Hello, Our lab is considering switching to denatured alcohol as a cost saving initiative. Is anyone using denatured alcohols and performing subsequent Class II IHC testing with no impact to results? Thank-you, Joanna Joanna Bartczak MLT II - Immunohistochemistry Calgary Laboratory Services 403-770-3695 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From liz <@t> premierlab.com Wed Feb 4 15:28:19 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Feb 4 15:28:26 2015 Subject: [Histonet] RE: use of denatured alcohol In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECE0AF@SBS2K8.premierlab.local> Joanna We are a research lab and have been using denatured alcohol for years, since 1998 we also recycle and have not seen any impact on our IHC staining. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanna Bartczak Sent: Wednesday, February 04, 2015 2:25 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] use of denatured alcohol Hello, Our lab is considering switching to denatured alcohol as a cost saving initiative. Is anyone using denatured alcohols and performing subsequent Class II IHC testing with no impact to results? Thank-you, Joanna Joanna Bartczak MLT II - Immunohistochemistry Calgary Laboratory Services 403-770-3695 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rooki.parak <@t> gmail.com Thu Feb 5 04:01:41 2015 From: rooki.parak <@t> gmail.com (Rooki Parak) Date: Thu Feb 5 04:01:45 2015 Subject: [Histonet] Substitute for Safran du Gatinais Message-ID: I am doing the Movats Pentachrome stain which requires the use of Safran du Gatinais. I would like to know if there is any substitute for Safran du Gatinais. From talulahgosh <@t> gmail.com Thu Feb 5 05:23:05 2015 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Thu Feb 5 05:23:18 2015 Subject: [Histonet] paraffin sectioning-dry tissue? Message-ID: Hello all! I just started sectioning mouse liver in paraffin and the tissue is very dry. I know it's not supposed to have water due to the processing, but the weird thing is that one tech's solution is to put a wet kimwipe on the block for a while. It seems to me that there is a larger processing issue if this is happening, am I correct? And why add water when you've already dehydrated it? Unfortunately, we do not have the set up to embed them ourselves, so we have to send them to a histology lab. They were sectioning for us, but they are backlogged, so my boss wants me to do it. Therefore, I can't tell you how they were processed, but I think usually the histology lab manages to get good sections. Is putting a wet kimwipe (using distilled water) the best way to get rid of chatter that's only in the tissue? The surrounding paraffin sections excellent. This may have been answered already, but a very quick google search didn't help. My googlefu is probably erratic as it's still early. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" From mjones <@t> metropath.com Thu Feb 5 08:02:24 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Thu Feb 5 08:02:33 2015 Subject: [Histonet] paraffin sectioning-dry tissue? In-Reply-To: References: Message-ID: After macro trimming into our blocks (very gently if friable or dry) we place them on an ice tray to soak for a good while. Each well is filled with a little bit of water - this rehydrates the tissue just enough to get a few good sections off the top. we?ve had success with this method for most any tissue (the more dry the tissue, the longer the soak). Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 2/5/15, 4:23 AM, "Emily Brown" wrote: >Hello all! > >I just started sectioning mouse liver in paraffin and the tissue is very >dry. I know it's not supposed to have water due to the processing, but >the >weird thing is that one tech's solution is to put a wet kimwipe on the >block for a while. >It seems to me that there is a larger processing issue if this is >happening, am I correct? And why add water when you've already dehydrated >it? >Unfortunately, we do not have the set up to embed them ourselves, so we >have to send them to a histology lab. They were sectioning for us, but >they are backlogged, so my boss wants me to do it. Therefore, I can't >tell >you how they were processed, but I think usually the histology lab manages >to get good sections. >Is putting a wet kimwipe (using distilled water) the best way to get rid >of >chatter that's only in the tissue? The surrounding paraffin sections >excellent. >This may have been answered already, but a very quick google search didn't >help. My googlefu is probably erratic as it's still early. > >Emily > > >"By bitching and bitching and bitching, they could exhaust the drama of >their own horror stories. Grow bored. Only then could they accept a new >story for their lives. Move forward." > >-Chuck Palahniuk, "Haunted" >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joanna.Bartczak <@t> cls.ab.ca Wed Feb 4 14:58:52 2015 From: Joanna.Bartczak <@t> cls.ab.ca (Joanna Bartczak) Date: Thu Feb 5 08:08:08 2015 Subject: [Histonet] use of denatured alcohol Message-ID: Hello, Our lab is considering switching to denatured alcohol as a cost saving initiative. Is anyone using denatured alcohols and performing subsequent Class II IHC testing with no impact to results? Thank-you, Joanna Joanna Bartczak MLT II - Immunohistochemistry Calgary Laboratory Services 403-770-3695 ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From mcauliff <@t> rwjms.rutgers.edu Thu Feb 5 08:29:25 2015 From: mcauliff <@t> rwjms.rutgers.edu (Geoff) Date: Thu Feb 5 08:29:37 2015 Subject: [Histonet] paraffin sectioning-dry tissue? In-Reply-To: References: Message-ID: <54D37E45.7090402@umdnj.edu> This is common with mouse and rat tissues, they get "over-dried" with a typical processing schedule. Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more. Geoff On 2/5/2015 6:23 AM, Emily Brown wrote: > Hello all! > > I just started sectioning mouse liver in paraffin and the tissue is very > dry. I know it's not supposed to have water due to the processing, but the > weird thing is that one tech's solution is to put a wet kimwipe on the > block for a while. > It seems to me that there is a larger processing issue if this is > happening, am I correct? And why add water when you've already dehydrated > it? > Unfortunately, we do not have the set up to embed them ourselves, so we > have to send them to a histology lab. They were sectioning for us, but > they are backlogged, so my boss wants me to do it. Therefore, I can't tell > you how they were processed, but I think usually the histology lab manages > to get good sections. > Is putting a wet kimwipe (using distilled water) the best way to get rid of > chatter that's only in the tissue? The surrounding paraffin sections > excellent. > This may have been answered already, but a very quick google search didn't > help. My googlefu is probably erratic as it's still early. > > Emily > > > "By bitching and bitching and bitching, they could exhaust the drama of > their own horror stories. Grow bored. Only then could they accept a new > story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff@rwjms.rutgers.edu ********************************************** From mills <@t> 3scan.com Thu Feb 5 08:41:06 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Thu Feb 5 08:41:17 2015 Subject: [Histonet] paraffin sectioning-dry tissue? In-Reply-To: <54D37E45.7090402@umdnj.edu> References: <54D37E45.7090402@umdnj.edu> Message-ID: Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can be really dry and needs a 'soak'. I have left them for an hour before now but don't leave it for longer than 4 hours though because it can start to swell and de-process! You will still only get a few non-chattery sections so be gentle. Thinner sections also help too (3-4.5). Plus low um polishing after you trim Good luck! It is weird at first but you will get used to it! Caroline Sent from my iPhone > On Feb 5, 2015, at 6:29 AM, Geoff wrote: > > This is common with mouse and rat tissues, they get "over-dried" with a typical processing schedule. > Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more. > > Geoff > >> On 2/5/2015 6:23 AM, Emily Brown wrote: >> Hello all! >> >> I just started sectioning mouse liver in paraffin and the tissue is very >> dry. I know it's not supposed to have water due to the processing, but the >> weird thing is that one tech's solution is to put a wet kimwipe on the >> block for a while. >> It seems to me that there is a larger processing issue if this is >> happening, am I correct? And why add water when you've already dehydrated >> it? >> Unfortunately, we do not have the set up to embed them ourselves, so we >> have to send them to a histology lab. They were sectioning for us, but >> they are backlogged, so my boss wants me to do it. Therefore, I can't tell >> you how they were processed, but I think usually the histology lab manages >> to get good sections. >> Is putting a wet kimwipe (using distilled water) the best way to get rid of >> chatter that's only in the tissue? The surrounding paraffin sections >> excellent. >> This may have been answered already, but a very quick google search didn't >> help. My googlefu is probably erratic as it's still early. >> >> Emily >> >> >> "By bitching and bitching and bitching, they could exhaust the drama of >> their own horror stories. Grow bored. Only then could they accept a new >> story for their lives. Move forward." >> >> -Chuck Palahniuk, "Haunted" >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732) 235-4583; fax: -4029 > mcauliff@rwjms.rutgers.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Feb 5 08:54:05 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 5 08:54:26 2015 Subject: [Histonet] C-Kit Message-ID: Hi All, I have a pathologist that is wanting me to bring in C-kit. My question is am I required by CAP to also do the Survey for predicative markers for C-kit? Thanks for your time and answers. Mike From Nancy.Stedman <@t> buschgardens.com Thu Feb 5 08:54:27 2015 From: Nancy.Stedman <@t> buschgardens.com (Stedman, Nancy) Date: Thu Feb 5 08:54:40 2015 Subject: [Histonet] Looking for ribbons for old Surgipath VCP cassette printer Message-ID: <18D791D4EE07BC41BF05F8EF3CCCDE24678A83A1@FTCSEAP4001.nam.int.local> Hi everyone - For those of us who are still in the dark ages... have an old Surgipath VCP cassette printer; does anyone know where to get replacement ribbons for these machines these days? Does anyone know who supports these machines anymore? Thanks so much - -Nancy Stedman From JWatson <@t> gnf.org Thu Feb 5 09:35:23 2015 From: JWatson <@t> gnf.org (James Watson) Date: Thu Feb 5 09:35:31 2015 Subject: [Histonet] paraffin sectioning-dry tissue? In-Reply-To: References: <54D37E45.7090402@umdnj.edu> Message-ID: We use a 5% glycerin in denatured alcohol for our 100% alcohol on our tissue processor for routine animal tissues, this reduces the over dehydration of the animal tissue and greatly reduces the time required to soak the blocks. Warning, if processing fat or cell pellets do not use this, we switch the containers to straight 100% reagent alcohol for them. For fat we have a longer processing schedule and for cell pellets we have a short processing cycle. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Miller Sent: Thursday, February 05, 2015 6:41 AM To: Geoff Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] paraffin sectioning-dry tissue? Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, can be really dry and needs a 'soak'. I have left them for an hour before now but don't leave it for longer than 4 hours though because it can start to swell and de-process! You will still only get a few non-chattery sections so be gentle. Thinner sections also help too (3-4.5). Plus low um polishing after you trim Good luck! It is weird at first but you will get used to it! Caroline Sent from my iPhone > On Feb 5, 2015, at 6:29 AM, Geoff wrote: > > This is common with mouse and rat tissues, they get "over-dried" with a typical processing schedule. > Soaking the face of the block with a kimwipe wet with ice water for 60 -120 seconds will enable you to cut 10 nice sections, maybe more. > > Geoff > >> On 2/5/2015 6:23 AM, Emily Brown wrote: >> Hello all! >> >> I just started sectioning mouse liver in paraffin and the tissue is >> very dry. I know it's not supposed to have water due to the >> processing, but the weird thing is that one tech's solution is to put >> a wet kimwipe on the block for a while. >> It seems to me that there is a larger processing issue if this is >> happening, am I correct? And why add water when you've already >> dehydrated it? >> Unfortunately, we do not have the set up to embed them ourselves, so >> we have to send them to a histology lab. They were sectioning for >> us, but they are backlogged, so my boss wants me to do it. >> Therefore, I can't tell you how they were processed, but I think >> usually the histology lab manages to get good sections. >> Is putting a wet kimwipe (using distilled water) the best way to get >> rid of chatter that's only in the tissue? The surrounding paraffin >> sections excellent. >> This may have been answered already, but a very quick google search >> didn't help. My googlefu is probably erratic as it's still early. >> >> Emily >> >> >> "By bitching and bitching and bitching, they could exhaust the drama >> of their own horror stories. Grow bored. Only then could they accept >> a new story for their lives. Move forward." >> >> -Chuck Palahniuk, "Haunted" >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732) 235-4583; fax: -4029 > mcauliff@rwjms.rutgers.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Thu Feb 5 09:44:43 2015 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Feb 5 09:45:12 2015 Subject: [Histonet] Looking for ribbons for old Surgipath VCP cassette printer In-Reply-To: <18D791D4EE07BC41BF05F8EF3CCCDE24678A83A1@FTCSEAP4001.nam.int.local> References: <18D791D4EE07BC41BF05F8EF3CCCDE24678A83A1@FTCSEAP4001.nam.int.local> Message-ID: We have one too. We have been getting ribbons from Surgipath/Leica. The number is 38V0590001F. we have Tech One Biomedical service it. They have been great. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stedman, Nancy Sent: Thursday, February 05, 2015 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for ribbons for old Surgipath VCP cassette printer Hi everyone - For those of us who are still in the dark ages... have an old Surgipath VCP cassette printer; does anyone know where to get replacement ribbons for these machines these days? Does anyone know who supports these machines anymore? Thanks so much - -Nancy Stedman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NIngrao <@t> KaleidaHealth.Org Thu Feb 5 10:19:14 2015 From: NIngrao <@t> KaleidaHealth.Org (Ingrao, Nicholas) Date: Thu Feb 5 10:19:22 2015 Subject: [Histonet] Leica ASP basket transport In-Reply-To: References: Message-ID: Our lab has standardized all cassette racking into the Leica ASP 300 basket. For locations that ship back to the main lab I have not found a suitable hard plastic storage container that fits this basket. I performed an in-person search at home depot and lowes without success. 14047643576 Basket set Leica ASP300 S Looking to see what others are using for transport of this particular style of basket. I am struggling to find plastic storage container that will stack 3 high on the hook. Each basket is approx. 8x13x3 inches. Thanks, Nick Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida HealthÂ’s Technology Assistance Center at (716) 859-7777. From suetp918 <@t> comcast.net Thu Feb 5 10:31:32 2015 From: suetp918 <@t> comcast.net (suetp918) Date: Thu Feb 5 10:31:52 2015 Subject: [Histonet] Leica ASP basket transport Message-ID: <5y7ekvgfw7s0ovobjp2h7wgp.1423153892344@email.android.com> Good luck probably some type of plastic ware that u can find at wallmart Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: "Ingrao, Nicholas" Date:02/05/2015 11:19 AM (GMT-05:00) To: "'histonet@lists.utsouthwestern.edu'" Subject: [Histonet] Leica ASP basket transport Our lab has standardized all cassette racking into the Leica ASP 300 basket. For locations that ship back to the main lab I have not found a suitable hard plastic storage container that fits this basket. I performed an in-person search at home depot and lowes without success. 14047643576 Basket set Leica ASP300 S Looking to see what others are using for transport of this particular style of basket. I am struggling to find plastic storage container that will stack 3 high on the hook. Each basket is approx. 8x13x3 inches. Thanks, Nick Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health??s Technology Assistance Center at (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Thu Feb 5 10:45:30 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Feb 5 10:45:42 2015 Subject: [Histonet] RE: Leica ASP basket transport In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367F16E6@ex07.net.ucsf.edu> Glad to know I'm not the only one who haunts the plasticware sections of stores looking for the ideal container for the lab! We use some Rubbermaid containers for the Peloris and VIP baskets, but they are not fully 3" high. I suggest looking at Bed Bath and Beyond, Container Store, all supermarkets, etc. I looked for many months for the ideal container to hold our EM embedding resins bottles and finally found it at the Container Store. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingrao, Nicholas Sent: Thursday, February 05, 2015 8:19 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica ASP basket transport Our lab has standardized all cassette racking into the Leica ASP 300 basket. For locations that ship back to the main lab I have not found a suitable hard plastic storage container that fits this basket. I performed an in-person search at home depot and lowes without success. 14047643576 Basket set Leica ASP300 S Looking to see what others are using for transport of this particular style of basket. I am struggling to find plastic storage container that will stack 3 high on the hook. Each basket is approx. 8x13x3 inches. Thanks, Nick Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health??s Technology Assistance Center at (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lguernsey <@t> ucsd.edu Thu Feb 5 11:22:16 2015 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Thu Feb 5 11:23:08 2015 Subject: [Histonet] paraffin sectioning-dry tissue? In-Reply-To: References: <54D37E45.7090402@umdnj.edu> Message-ID: By adding water, ice, or warm humidity (through exhalations) to the mix, though, doesn't the block contract/expand? Wouldn't that change the ultimate thickness of the section? I've always wondered how much it affects things. Could your sections be 1 um (or multiple um!) thicker/thinner that you expect? What happens if individual blocks contract/expand differently (due to the amount of time left soaking, how far into the block you've cut since you last soaked, etc.)? I feel like you couldn't properly compare quantifications across a study if the thickness of your tissue is an unknown variable. Am I overthinking this? Lucie Guernsey UC San Diego (858) 822-5797 lguernsey@ucsd.edu On Thu, Feb 5, 2015 at 7:35 AM, James Watson wrote: > We use a 5% glycerin in denatured alcohol for our 100% alcohol on our > tissue processor for routine animal tissues, this reduces the over > dehydration of the animal tissue and greatly reduces the time required to > soak the blocks. Warning, if processing fat or cell pellets do not use > this, we switch the containers to straight 100% reagent alcohol for them. > For fat we have a longer processing schedule and for cell pellets we have a > short processing cycle. > > James Watson HT ASCP > GNF Genomics Institute of the Novartis Research Foundation > Scientific Technical Leader II, Histology > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Miller > Sent: Thursday, February 05, 2015 6:41 AM > To: Geoff > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] paraffin sectioning-dry tissue? > > Yes, exactly what Mike and Geoff said. All mouse tissue, especially liver, > can be really dry and needs a 'soak'. I have left them for an hour before > now but don't leave it for longer than 4 hours though because it can start > to swell and de-process! > > You will still only get a few non-chattery sections so be gentle. Thinner > sections also help too (3-4.5). Plus low um polishing after you trim > > Good luck! It is weird at first but you will get used to it! > > Caroline > > Sent from my iPhone > > > On Feb 5, 2015, at 6:29 AM, Geoff wrote: > > > > This is common with mouse and rat tissues, they get "over-dried" with a > typical processing schedule. > > Soaking the face of the block with a kimwipe wet with ice water for 60 > -120 seconds will enable you to cut 10 nice sections, maybe more. > > > > Geoff > > > >> On 2/5/2015 6:23 AM, Emily Brown wrote: > >> Hello all! > >> > >> I just started sectioning mouse liver in paraffin and the tissue is > >> very dry. I know it's not supposed to have water due to the > >> processing, but the weird thing is that one tech's solution is to put > >> a wet kimwipe on the block for a while. > >> It seems to me that there is a larger processing issue if this is > >> happening, am I correct? And why add water when you've already > >> dehydrated it? > >> Unfortunately, we do not have the set up to embed them ourselves, so > >> we have to send them to a histology lab. They were sectioning for > >> us, but they are backlogged, so my boss wants me to do it. > >> Therefore, I can't tell you how they were processed, but I think > >> usually the histology lab manages to get good sections. > >> Is putting a wet kimwipe (using distilled water) the best way to get > >> rid of chatter that's only in the tissue? The surrounding paraffin > >> sections excellent. > >> This may have been answered already, but a very quick google search > >> didn't help. My googlefu is probably erratic as it's still early. > >> > >> Emily > >> > >> > >> "By bitching and bitching and bitching, they could exhaust the drama > >> of their own horror stories. Grow bored. Only then could they accept > >> a new story for their lives. Move forward." > >> > >> -Chuck Palahniuk, "Haunted" > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > -- > > ********************************************** > > Geoff McAuliffe, Ph.D. > > Neuroscience and Cell Biology > > Robert Wood Johnson Medical School > > 675 Hoes Lane, Piscataway, NJ 08854 > > voice: (732) 235-4583; fax: -4029 > > mcauliff@rwjms.rutgers.edu > > ********************************************** > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Thu Feb 5 11:48:54 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Feb 5 11:49:15 2015 Subject: [Histonet] paraffin sectioning-dry tissue? - long response - image analysis related Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECE0BC@SBS2K8.premierlab.local> Lucie You are not overthinking this at all if you are utilizing your sections for any image analysis applications. You need to be able to standardize as much of the histology process as possible. There are so many other parameters that can cause section thickness to fluctuate. Such as the sharpness of your knife, how fast you turn the hand wheel. Blowing on the block is not acceptable in our lab that will create thicker sections since you are warming up the block. Great care is taken to standardize what we do, from soaking blocks to how we collect the sections to placement on the slide, and how often we move our knife blade. We routinely soak all of our blocks but we keep in mind so many other factors when we are providing histology for image analysis. It starts at the beginning with fixation, it all has to be standardized, our goal is to decrease the potential for variability. That is on our minds at every step through the histology process. The other thing to consider is how well the algorithm functions - you need to determine the limits of the algorithm and when it will stop functioning properly, which is usually due to staining issues (over or under staining or inconsistent staining), section thickness and overall section and stain quality which is so important. As a part of algorithm validation we test for these parameters we want to understand where we lose functionality, accuracy and precision of the algorithm. So we look at different section thicknesses and how that impacts analysis, we look at over and under staining parameters to see how that affects the algorithm, etc. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Thursday, February 05, 2015 10:22 AM To: James Watson Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] paraffin sectioning-dry tissue? By adding water, ice, or warm humidity (through exhalations) to the mix, though, doesn't the block contract/expand? Wouldn't that change the ultimate thickness of the section? I've always wondered how much it affects things. Could your sections be 1 um (or multiple um!) thicker/thinner that you expect? What happens if individual blocks contract/expand differently (due to the amount of time left soaking, how far into the block you've cut since you last soaked, etc.)? I feel like you couldn't properly compare quantifications across a study if the thickness of your tissue is an unknown variable. Am I overthinking this? Lucie Guernsey UC San Diego (858) 822-5797 lguernsey@ucsd.edu On Thu, Feb 5, 2015 at 7:35 AM, James Watson wrote: > We use a 5% glycerin in denatured alcohol for our 100% alcohol on our > tissue processor for routine animal tissues, this reduces the over > dehydration of the animal tissue and greatly reduces the time required > to soak the blocks. Warning, if processing fat or cell pellets do not > use this, we switch the containers to straight 100% reagent alcohol for them. > For fat we have a longer processing schedule and for cell pellets we > have a short processing cycle. > > James Watson HT ASCP > GNF Genomics Institute of the Novartis Research Foundation Scientific > Technical Leader II, Histology > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline > Miller > Sent: Thursday, February 05, 2015 6:41 AM > To: Geoff > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] paraffin sectioning-dry tissue? > > Yes, exactly what Mike and Geoff said. All mouse tissue, especially > liver, can be really dry and needs a 'soak'. I have left them for an > hour before now but don't leave it for longer than 4 hours though > because it can start to swell and de-process! > > You will still only get a few non-chattery sections so be gentle. > Thinner sections also help too (3-4.5). Plus low um polishing after > you trim > > Good luck! It is weird at first but you will get used to it! > > Caroline > > Sent from my iPhone > > > On Feb 5, 2015, at 6:29 AM, Geoff wrote: > > > > This is common with mouse and rat tissues, they get "over-dried" > > with a > typical processing schedule. > > Soaking the face of the block with a kimwipe wet with ice water for > > 60 > -120 seconds will enable you to cut 10 nice sections, maybe more. > > > > Geoff > > > >> On 2/5/2015 6:23 AM, Emily Brown wrote: > >> Hello all! > >> > >> I just started sectioning mouse liver in paraffin and the tissue is > >> very dry. I know it's not supposed to have water due to the > >> processing, but the weird thing is that one tech's solution is to > >> put a wet kimwipe on the block for a while. > >> It seems to me that there is a larger processing issue if this is > >> happening, am I correct? And why add water when you've already > >> dehydrated it? > >> Unfortunately, we do not have the set up to embed them ourselves, > >> so we have to send them to a histology lab. They were sectioning > >> for us, but they are backlogged, so my boss wants me to do it. > >> Therefore, I can't tell you how they were processed, but I think > >> usually the histology lab manages to get good sections. > >> Is putting a wet kimwipe (using distilled water) the best way to > >> get rid of chatter that's only in the tissue? The surrounding > >> paraffin sections excellent. > >> This may have been answered already, but a very quick google search > >> didn't help. My googlefu is probably erratic as it's still early. > >> > >> Emily > >> > >> > >> "By bitching and bitching and bitching, they could exhaust the > >> drama of their own horror stories. Grow bored. Only then could they > >> accept a new story for their lives. Move forward." > >> > >> -Chuck Palahniuk, "Haunted" > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > -- > > ********************************************** > > Geoff McAuliffe, Ph.D. > > Neuroscience and Cell Biology > > Robert Wood Johnson Medical School > > 675 Hoes Lane, Piscataway, NJ 08854 > > voice: (732) 235-4583; fax: -4029 > > mcauliff@rwjms.rutgers.edu > > ********************************************** > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From o.m.gallagher <@t> sheffield.ac.uk Thu Feb 5 11:55:49 2015 From: o.m.gallagher <@t> sheffield.ac.uk (Orla M Gallagher) Date: Thu Feb 5 11:56:06 2015 Subject: [Histonet] Cryosectioning undecalcified bone Message-ID: Dear Histonetters, We would like to develop cryosectioning for undecalcified mouse and rat bones. Previous attempts to use the CryoJane system from Instrumedics with an old Bright cryostat and solid tungsten carbide blade a few years ago didn't result in successful reproducible sections - marrow without bone or bone without marrow on the slide, in spite of various freezing and prep. protocols used by my colleague. I suspect the old cryostat and blade weren't helping either. Have you any recommendations on the best cryostat to use to do this? We'd like to also use the cryostat for standard soft tissue sectioning. I've seen Leica mentioned in a few papers relating to CryoJane. There appears also to be a tape transfer method from Kawamoto's Section-Lab Co. Ltd. (info@section-lab.jp) Does anyone use this method or know whether the consumables are still available for purchase, as the website seems dormant? Thanks for any advice, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/humanmetabolism/greenimpact http://www.sheffield.ac.uk/visitors/mapsandtravel From lpjones <@t> srhs-pa.org Thu Feb 5 12:02:51 2015 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Thu Feb 5 12:07:45 2015 Subject: [Histonet] RE: Leica ASP basket transport In-Reply-To: <761E2B5697F795489C8710BCC72141FF367F16E6@ex07.net.ucsf.edu> References: , <761E2B5697F795489C8710BCC72141FF367F16E6@ex07.net.ucsf.edu> Message-ID: Not sure what your baskets look like, but we found "Rubbermaid-like" travel shoe boxes at Walmart that work really well for us. They're deeper than what we found in the kitchen department. Look by the luggage and travel stuff. (We also haunt the plasticware sections of stores... :D) Laura Jones B.A., HT, PBT (ASCP) | Lead Tech, Histology | Community Health Systems 740 East State Street | Sharon PA | Phone (724)983-3950 | Fax (724)983-3982 www.sharonregional.com | lpjones@srhs-pa.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [Timothy.Morken@ucsf.edu] Sent: Thursday, February 05, 2015 11:45 AM To: 'Ingrao, Nicholas'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Leica ASP basket transport Glad to know I'm not the only one who haunts the plasticware sections of stores looking for the ideal container for the lab! We use some Rubbermaid containers for the Peloris and VIP baskets, but they are not fully 3" high. I suggest looking at Bed Bath and Beyond, Container Store, all supermarkets, etc. I looked for many months for the ideal container to hold our EM embedding resins bottles and finally found it at the Container Store. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingrao, Nicholas Sent: Thursday, February 05, 2015 8:19 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Leica ASP basket transport Our lab has standardized all cassette racking into the Leica ASP 300 basket. For locations that ship back to the main lab I have not found a suitable hard plastic storage container that fits this basket. I performed an in-person search at home depot and lowes without success. 14047643576 Basket set Leica ASP300 S Looking to see what others are using for transport of this particular style of basket. I am struggling to find plastic storage container that will stack 3 high on the hook. Each basket is approx. 8x13x3 inches. Thanks, Nick Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health??s Technology Assistance Center at (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From liz <@t> premierlab.com Thu Feb 5 12:07:42 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Feb 5 12:07:48 2015 Subject: [Histonet] Cryosectioning undecalcified bone In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECE0BF@SBS2K8.premierlab.local> Orla There is an article in the Journal of Histotechnology from a while ago from John Tarpley that addressed methods for this. I have a pdf of it and I will send in another e-mail. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher Sent: Thursday, February 05, 2015 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryosectioning undecalcified bone Dear Histonetters, We would like to develop cryosectioning for undecalcified mouse and rat bones. Previous attempts to use the CryoJane system from Instrumedics with an old Bright cryostat and solid tungsten carbide blade a few years ago didn't result in successful reproducible sections - marrow without bone or bone without marrow on the slide, in spite of various freezing and prep. protocols used by my colleague. I suspect the old cryostat and blade weren't helping either. Have you any recommendations on the best cryostat to use to do this? We'd like to also use the cryostat for standard soft tissue sectioning. I've seen Leica mentioned in a few papers relating to CryoJane. There appears also to be a tape transfer method from Kawamoto's Section-Lab Co. Ltd. (info@section-lab.jp) Does anyone use this method or know whether the consumables are still available for purchase, as the website seems dormant? Thanks for any advice, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/humanmetabolism/greenimpact http://www.sheffield.ac.uk/visitors/mapsandtravel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Thu Feb 5 12:44:02 2015 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Feb 5 12:44:11 2015 Subject: [Histonet] Cryosectioning undecalcified bone In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECE0BF@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECE0BF@SBS2K8.premierlab.local> Message-ID: Hello, I believe that the products for the CryoJane tape transfer are still available from Fisher. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St? Room 310 Basement| Bond St Annex Building Baltimore, MD?| 21231 410-614-1660 http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, February 05, 2015 1:08 PM To: Orla M Gallagher; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cryosectioning undecalcified bone Orla There is an article in the Journal of Histotechnology from a while ago from John Tarpley that addressed methods for this. I have a pdf of it and I will send in another e-mail. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher Sent: Thursday, February 05, 2015 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryosectioning undecalcified bone Dear Histonetters, We would like to develop cryosectioning for undecalcified mouse and rat bones. Previous attempts to use the CryoJane system from Instrumedics with an old Bright cryostat and solid tungsten carbide blade a few years ago didn't result in successful reproducible sections - marrow without bone or bone without marrow on the slide, in spite of various freezing and prep. protocols used by my colleague. I suspect the old cryostat and blade weren't helping either. Have you any recommendations on the best cryostat to use to do this? We'd like to also use the cryostat for standard soft tissue sectioning. I've seen Leica mentioned in a few papers relating to CryoJane. There appears also to be a tape transfer method from Kawamoto's Section-Lab Co. Ltd. (info@section-lab.jp) Does anyone use this method or know whether the consumables are still available for purchase, as the website seems dormant? Thanks for any advice, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/humanmetabolism/greenimpact http://www.sheffield.ac.uk/visitors/mapsandtravel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Catherine.L.Scott <@t> uth.tmc.edu Thu Feb 5 13:45:23 2015 From: Catherine.L.Scott <@t> uth.tmc.edu (Scott, Catherine L) Date: Thu Feb 5 13:45:32 2015 Subject: [Histonet] Rick Message-ID: <4571339ED5505F4F8AD51D2280C4C0EA162238CE@UTHMAIL2.uthouston.edu> Rick had a situation at home and had to leave about noon From Catherine.L.Scott <@t> uth.tmc.edu Thu Feb 5 13:47:00 2015 From: Catherine.L.Scott <@t> uth.tmc.edu (Scott, Catherine L) Date: Thu Feb 5 13:47:39 2015 Subject: [Histonet] Recall: Rick Message-ID: <4571339ED5505F4F8AD51D2280C4C0EA162238EB@UTHMAIL2.uthouston.edu> Scott, Catherine L would like to recall the message, "Rick". From BKChaffee <@t> mdanderson.org Thu Feb 5 13:53:28 2015 From: BKChaffee <@t> mdanderson.org (Chaffee,Beth K) Date: Thu Feb 5 13:53:36 2015 Subject: [Histonet] Job opportunity: Research Histology Technician in Bastrop, TX Message-ID: <6E57F9B5D7D5264DB3B6C2F3C02050E228DE93AA@DCPWPEXMBX03.mdanderson.edu> Job opportunity for a research histology technician at MD Anderson Cancer Center at the BASTROP, TX location. (approximately 35 miles from Austin, TX) http://jobs.mdanderson.org/texas/research/jobid6803661-research-histology-technician-bastrop-texas-jobs Beth K. Chaffee, DVM, PhD, DACVP Assistant Professor Michale E. Keeling Center for Comparative Medicine and Research MD Anderson Cancer Center 650 Cool Water Dr. Bastrop, TX 78602 Tel. (512) 321-3991 Fax (512) 332-5397 bkchaffee@mdanderson.org From melsmith <@t> udel.edu Thu Feb 5 14:32:41 2015 From: melsmith <@t> udel.edu (Melanie Smith) Date: Thu Feb 5 14:33:04 2015 Subject: [Histonet] TRAP staining FFPE sections Message-ID: Hi all, I just performed TRAP staining on ffpe edta delcaled bone sections using the Sigma kit (#387A), and was wondering if anyone had experience with reducing the yellow staining over the entire tissue due to the Fast Garnet GBC. I had positive TRAP staining that is red, but there is a lack of contrast due to the yellow that I'd like to improve. Thanks! -- Melanie Smith, MS melsmith@udel.edu From blayjorge <@t> gmail.com Thu Feb 5 14:57:07 2015 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Thu Feb 5 14:57:16 2015 Subject: [Histonet] denatured alcohol Message-ID: Dear Histonetters: The other day, someone sent me a sample in (allegedly) denaturated alcohol. I asked the colleague to let me know what was the concentration of ethanol in the sample and the answer was "about 80%". From the smell, I suspect the rest is methanol. I need to dehydrate the sample through an ethanol series. Could someone recommend where should I get started? OK to begin at 80% or to assume that the sample is dehydrated enough in this denaturated alcohol. Thereafter, I will process the sample via a critical point dryer. If you have any suggestions, please email me directly at blayjorge@gmail.com . Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From blayjorge <@t> gmail.com Thu Feb 5 15:01:39 2015 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Thu Feb 5 15:01:48 2015 Subject: [Histonet] Troponin is biomarker of heart attacks Message-ID: Dear Histonnetters: I am not sure the range of expertise of the members may reach this far but let me try. Question: Could sometone tell me (blayjorge@gmail.com) why is blood troponin is biomarker of heart attacks. I am aware that is is not the only biomarker but nowhere have I yet seen an explanation why not tropomyosin of other protein components of the cardiac muscle that may find themselves in the blood stream as a result of damage or death due to the heart attack. If you know of a human physiology / human anatomy listserver, please, let me know. Gratefully, Jorge blayjorge@gmail.com -- Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From rjbuesa <@t> yahoo.com Thu Feb 5 15:18:10 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 5 15:18:14 2015 Subject: [Histonet] denatured alcohol In-Reply-To: References: Message-ID: <277293547.282607.1423171090059.JavaMail.yahoo@mail.yahoo.com> You will have to start in 80% "ethanol" and you cannot assume the tissue is dehydrated enough.Ren? J.? On Thursday, February 5, 2015 3:57 PM, Jorge A. Santiago-Blay wrote: Dear Histonetters: The other day, someone sent me a sample in (allegedly) denaturated alcohol. I asked the colleague to let me know what was the concentration of ethanol in the sample and the answer was "about 80%". From the smell, I suspect the rest is methanol. I need to dehydrate the sample through an ethanol series. Could someone recommend where should I get started? OK to begin at 80% or to assume that the sample is dehydrated enough in this denaturated alcohol. Thereafter, I will process the sample via a critical point dryer.? If you have any suggestions, please email me directly at blayjorge@gmail.com . Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billions1998 <@t> outlook.com Thu Feb 5 17:36:27 2015 From: billions1998 <@t> outlook.com (billions1998@outlook.com) Date: Thu Feb 5 17:37:23 2015 Subject: [Histonet] Re: ALCIAN YELLOW ALCIAN BLUE 8GX References: Message-ID: Dear Sirs, We are manufacturer of ALCIAN BLUE 8GX and ALCIAN YELLOW. Looking forward to hearing from you asap. Kind Regards Minggeng Wang, Ph.D/President SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road, Suzhou New & Hi-Tech District, 215011 China Tel: 0086 512 68246939 Fax: 0086 512 68258994 Inquiries:sinoerachem@sina.cn General Questions: billions1998@outlook.com http://www.sinoeratech.com http://www.sinoerachem.com From b427297 <@t> aol.com Thu Feb 5 18:42:48 2015 From: b427297 <@t> aol.com (William J. O'Connor III) Date: Thu Feb 5 18:42:54 2015 Subject: [Histonet] Troponin is biomarker of heart attacks In-Reply-To: References: Message-ID: <14b5c56d1d2-10bb-4dd5@webprd-a12.mail.aol.com> troponin I is the only troponin assay to show cardiac muscle injuty. -----Original Message----- From: Jorge A. Santiago-Blay To: Histonet Sent: Thu, Feb 5, 2015 3:02 pm Subject: [Histonet] Troponin is biomarker of heart attacks Dear Histonnetters: I am not sure the range of expertise of the members may reach this far but let me try. Question: Could sometone tell me (blayjorge@gmail.com) why is blood troponin is biomarker of heart attacks. I am aware that is is not the only biomarker but nowhere have I yet seen an explanation why not tropomyosin of other protein components of the cardiac muscle that may find themselves in the blood stream as a result of damage or death due to the heart attack. If you know of a human physiology / human anatomy listserver, please, let me know. Gratefully, Jorge blayjorge@gmail.com -- Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Stedman <@t> buschgardens.com Thu Feb 5 19:42:12 2015 From: Nancy.Stedman <@t> buschgardens.com (Stedman, Nancy) Date: Thu Feb 5 19:42:22 2015 Subject: [Histonet] Troponin is biomarker of heart attacks In-Reply-To: References: Message-ID: <18D791D4EE07BC41BF05F8EF3CCCDE24678A8D2F@FTCSEAP4001.nam.int.local> Hi Jorge - If I understand your question correctly - it is because cardiac troponin is specific for cardiac muscle damage. Other muscle enzymes/proteins we can assay for could also be elevated with skeletal muscle damage. -Nancy Stedman ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Jorge A. Santiago-Blay [blayjorge@gmail.com] Sent: Thursday, February 05, 2015 4:01 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Troponin is biomarker of heart attacks Dear Histonnetters: I am not sure the range of expertise of the members may reach this far but let me try. Question: Could sometone tell me (blayjorge@gmail.com) why is blood troponin is biomarker of heart attacks. I am aware that is is not the only biomarker but nowhere have I yet seen an explanation why not tropomyosin of other protein components of the cardiac muscle that may find themselves in the blood stream as a result of damage or death due to the heart attack. If you know of a human physiology / human anatomy listserver, please, let me know. Gratefully, Jorge blayjorge@gmail.com -- Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Feb 6 01:23:26 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Feb 6 01:23:35 2015 Subject: [Histonet] test Message-ID: <002701d041dd$cc99e540$65cdafc0$@gmx.at> test mail - best wishes to all Gudrun From Lynne.Bell <@t> cvmc.org Fri Feb 6 05:50:47 2015 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Fri Feb 6 05:50:58 2015 Subject: [Histonet] Looking for ribbons for old Surgipath VCP cassette printer In-Reply-To: <18D791D4EE07BC41BF05F8EF3CCCDE24678A83A1@FTCSEAP4001.nam.int.local> References: <18D791D4EE07BC41BF05F8EF3CCCDE24678A83A1@FTCSEAP4001.nam.int.local> Message-ID: We are still in the dark ages too! We get our ribbons from Mercedes Medical, Item # PAP 79333, 12 ribbons in a box for $48 - much cheaper than Leica! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stedman, Nancy Sent: Thursday, February 05, 2015 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for ribbons for old Surgipath VCP cassette printer Hi everyone - For those of us who are still in the dark ages... have an old Surgipath VCP cassette printer; does anyone know where to get replacement ribbons for these machines these days? Does anyone know who supports these machines anymore? Thanks so much - -Nancy Stedman _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Fri Feb 6 11:12:38 2015 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Feb 6 11:12:52 2015 Subject: [Histonet] HNF-1 beta Message-ID: <77DD817201982748BC67D7960F2F76AF106DFA@UWHC-MBX12.uwhis.hosp.wisc.edu> Good morning, We have a pathologist that has a case he wants sent out for HMF-1? (hepatocyte necrosing factor-1?) . Are there any reference labs out there that offer this? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From LBUSTAMANTE <@t> cvm.tamu.edu Fri Feb 6 13:11:06 2015 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Fri Feb 6 13:11:12 2015 Subject: [Histonet] Hyde Away products Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC90150543665@CVMMB02.cvm.tamu.edu> Does anyone uses Formalin Neutralizer Hyde Away from Fisher?. Do you do any test to figure out if is ready?, all neutralize? Thank you very much. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From fiona.wright <@t> sheffield.ac.uk Thu Feb 5 10:10:15 2015 From: fiona.wright <@t> sheffield.ac.uk (Fiona J Wright) Date: Fri Feb 6 13:48:39 2015 Subject: [Histonet] decalcifying sheep knee joints Message-ID: Can anyone help me out with a problem Im going to have in a few months? I will be receiving some sheep joints for processing. They will need to be decalcified in a manner that will leave the tissue suitable for immunohistochemistry and special stains such as safranin O. How do I go about this? I have previously only worked with mouse bones and decalcified for several weeks in EDTA; this has always given me good histological results, but I fear for sheep bones this methodology would take months. Any advice gratefully received. Fiona Wright Dept of Infection and Immunity University of Sheffield K118, Medical School Beech Hill Road Sheffield S10 2RX fiona.wright@sheffield.ac.uk 0114 271 2102 07769 334438 From Philip.Dobson <@t> nuth.nhs.uk Fri Feb 6 11:42:26 2015 From: Philip.Dobson <@t> nuth.nhs.uk (Dobson, Philip) Date: Fri Feb 6 13:48:41 2015 Subject: [Histonet] Edta Message-ID: <20150206174228.6A2C7449F99@nhs-pd1e-esg102.ad1.nhs.net> Hi, Can anybody tell me the difference between tetra-edta hydrate and tetra-edta dihydrate for use as a decalcifying agent? Thanks in advance. Phil ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ******************************************************************************************************************** From martinw <@t> umn.edu Fri Feb 6 17:04:20 2015 From: martinw <@t> umn.edu (Martin Wessendorf) Date: Fri Feb 6 17:04:29 2015 Subject: [Histonet] myelin-stained sections of human brain for teaching lab Message-ID: <54D54874.30008@umn.edu> Dear Histo List-- I apologize if this is off-topic, but I'm looking for someone who could section human brain (--pyramidal decussation through genu of corpus callosum) and stain the sections using a myelin stain (e.g. a Weigert or Weil). We need these for teaching so we would want a total of roughly 50 sets; we'd want each set to sample (more-or-less!) the same 25 levels along the neuraxis. Thus we're talking about a total of 1250 stained sections, most of them big enough to need 2" x 3" slides. Can anyone out there recommend a histologist or organization that would have the capability (and hopefully also the interest!) to undertake a project like this? Off list is fine. Thank you for your help--I greatly appreciate it! Martin Wessendorf -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 e-mail:martinw@umn.edu From adesupo2002 <@t> hotmail.com Fri Feb 6 19:52:28 2015 From: adesupo2002 <@t> hotmail.com (ADESUPO ADESUYI) Date: Fri Feb 6 19:52:37 2015 Subject: [Histonet] FMEA Message-ID: Hi everyone, Does anyone out there have a research/professional articles on FMEA or on Specimen Labeling, Preventing Specimen Errors etc to share with me please. Thanks, Banjo Adesuyi NRHS Norman, OK From lsmallwo <@t> juno.com Fri Feb 6 21:37:23 2015 From: lsmallwo <@t> juno.com (lsmallwo@juno.com) Date: Fri Feb 6 21:39:11 2015 Subject: [Histonet] Frozen Sections Message-ID: <20150206.223723.14830.0@webmail02.vgs.untd.com> Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 From Histotech <@t> imagesbyhopper.com Sat Feb 7 08:30:34 2015 From: Histotech <@t> imagesbyhopper.com (Michelle) Date: Sat Feb 7 08:30:49 2015 Subject: [Histonet] CLIA Inspection Question Message-ID: <00c701d042e2$a2988110$e7c98330$@imagesbyhopper.com> Hi Histonetters, It's me again! ;) I am interested in your thoughts on this: would it be better to simply apply for a CAP accreditation and get both the CAP and CLIA certificates at the same time? My thoughts are these: if meeting the CAP guidelines is effectively meeting the CLIA requirements, would it make more sense to prep for one bird and get two at the same time? Would I still need to look at the CLIA regs, under this scenario? Out of my element, but definitely trying to learn! Thanks for the input I have received so far ... you all are a wonderful resource. :) Michelle From Histotech <@t> imagesbyhopper.com Sat Feb 7 08:43:13 2015 From: Histotech <@t> imagesbyhopper.com (Michelle) Date: Sat Feb 7 08:43:29 2015 Subject: [Histonet] test message Message-ID: <00c901d042e4$66c11150$344333f0$@imagesbyhopper.com> It seems every time I reply to a histonet email, it is followed up with a foreign character email. I have no idea why this could be happening, if, in fact it is related. I am just testing to see if a newly composed email will do the same thing? Thanks, Michelle From Histotech <@t> imagesbyhopper.com Sat Feb 7 09:01:24 2015 From: Histotech <@t> imagesbyhopper.com (Michelle) Date: Sat Feb 7 09:01:40 2015 Subject: [Histonet] it happened again - the foreign reply Message-ID: <00d101d042e6$f14cfc10$d3e6f430$@imagesbyhopper.com> Does anyone have a clue as to why I keep getting a "foreign" reply to every email I send to histonet? Is the group seeing the same foreign message I am seeing? Am I the only one getting this message? Michelle From mills <@t> 3scan.com Sat Feb 7 09:04:20 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Sat Feb 7 09:04:27 2015 Subject: [Histonet] test message In-Reply-To: <00c901d042e4$66c11150$344333f0$@imagesbyhopper.com> References: <00c901d042e4$66c11150$344333f0$@imagesbyhopper.com> Message-ID: <7FF4AE36-D9F1-4C83-A977-CEDB290E87B5@3scan.com> I think it is an anti-spam message. Asking you to add yourself to their list of 'safe' addresses. Is anyone on this list able to ask them to try to make it stop? Thanks! mills > On Feb 7, 2015, at 6:43 AM, Michelle wrote: > > It seems every time I reply to a histonet email, it is followed up with a > foreign character email. I have no idea why this could be happening, if, in > fact it is related. I am just testing to see if a newly composed email will > do the same thing? > > > > Thanks, > > Michelle > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Histotech <@t> imagesbyhopper.com Sat Feb 7 09:07:11 2015 From: Histotech <@t> imagesbyhopper.com (Michelle) Date: Sat Feb 7 09:07:37 2015 Subject: [Histonet] it happened again - the foreign reply In-Reply-To: <00d101d042e6$f14cfc10$d3e6f430$@imagesbyhopper.com> References: <00d101d042e6$f14cfc10$d3e6f430$@imagesbyhopper.com> Message-ID: <00d601d042e7$c2c603e0$48520ba0$@imagesbyhopper.com> Interestingly enough, after I removed the histonet email address from the "quick dropdown" of recently used addresses in my outlook "To" field and simply manually typed the address, I did not get the foreign reply. Related??? Who knows. I agree with Mills, it would be nice not to see those messages. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Sent: Saturday, February 7, 2015 10:01 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] it happened again - the foreign reply Does anyone have a clue as to why I keep getting a "foreign" reply to every email I send to histonet? Is the group seeing the same foreign message I am seeing? Am I the only one getting this message? Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat Feb 7 09:11:23 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Feb 7 09:11:40 2015 Subject: AW: [Histonet] it happened again - the foreign reply In-Reply-To: <00d601d042e7$c2c603e0$48520ba0$@imagesbyhopper.com> References: <00d101d042e6$f14cfc10$d3e6f430$@imagesbyhopper.com> <00d601d042e7$c2c603e0$48520ba0$@imagesbyhopper.com> Message-ID: <001401d042e8$57ed74d0$07c85e70$@gmx.at> I put the sender-adress of these unwanted mails to my "SPAM"s. So they are not annoying. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Michelle Gesendet: Samstag, 07. Februar 2015 16:07 An: Histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] it happened again - the foreign reply Interestingly enough, after I removed the histonet email address from the "quick dropdown" of recently used addresses in my outlook "To" field and simply manually typed the address, I did not get the foreign reply. Related??? Who knows. I agree with Mills, it would be nice not to see those messages. Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Sent: Saturday, February 7, 2015 10:01 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] it happened again - the foreign reply Does anyone have a clue as to why I keep getting a "foreign" reply to every email I send to histonet? Is the group seeing the same foreign message I am seeing? Am I the only one getting this message? Michelle _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From udsd007 <@t> gmail.com Sat Feb 7 09:24:19 2015 From: udsd007 <@t> gmail.com (Mike Andrews) Date: Sat Feb 7 09:24:29 2015 Subject: [Histonet] test message In-Reply-To: <7FF4AE36-D9F1-4C83-A977-CEDB290E87B5@3scan.com> References: <00c901d042e4$66c11150$344333f0$@imagesbyhopper.com> <7FF4AE36-D9F1-4C83-A977-CEDB290E87B5@3scan.com> Message-ID: <399715939A329FAF.02AC075E-A921-47E0-9ADE-3CE42ADADCB5@mail.outlook.com> It is exactly that: a subscriber in Korea is asking (in Korean) that you visit some website or take some other action to get through his spam filter. It is an antiquated and unfriendly technique which has been deprecated vigorously since it first saw light.? List manager, please remove the offending subscriber.? Mike Andrews, W5EGO Tired old Sysadmin gmikea@mikea.ath.cx _____________________________ From: Caroline Miller Sent: Saturday, February 7, 2015 9:04 AM Subject: Re: [Histonet] test message To: Michelle Cc: I think it is an anti-spam message. Asking you to add yourself to their list of 'safe' addresses. Is anyone on this list able to ask them to try to make it stop? Thanks!mills> On Feb 7, 2015, at 6:43 AM, Michelle wrote:> > It seems every time I reply to a histonet email, it is followed up with a> foreign character email. I have no idea why this could be happening, if, in> fact it is related. I am just testing to see if a newly composed email will> do the same thing? > > > > Thanks,> > Michelle> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills <@t> 3scan.com Sat Feb 7 09:38:44 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Sat Feb 7 09:38:52 2015 Subject: [Histonet] test message In-Reply-To: <399715939A329FAF.02AC075E-A921-47E0-9ADE-3CE42ADADCB5@mail.outlook.com> References: <00c901d042e4$66c11150$344333f0$@imagesbyhopper.com> <7FF4AE36-D9F1-4C83-A977-CEDB290E87B5@3scan.com> <399715939A329FAF.02AC075E-A921-47E0-9ADE-3CE42ADADCB5@mail.outlook.com> Message-ID: <393F3B34-4609-4847-A4E7-5A9FC6B72270@3scan.com> I never scrolled through their message before, but I just did and it has an English 'click here' link. I just added myself to their silly system. But I agree with The other email too, adding their account to your spam is also a good option :) mills Sent from my iPhone > On Feb 7, 2015, at 7:24 AM, Mike Andrews wrote: > > It is exactly that: a subscriber in Korea is asking (in Korean) that you visit some website or take some other action to get through his spam filter. It is an antiquated and unfriendly technique which has been deprecated vigorously since it first saw light. > List manager, please remove the offending subscriber. > > Mike Andrews, W5EGO > Tired old Sysadmin > gmikea@mikea.ath.cx > > _____________________________ > From: Caroline Miller > Sent: Saturday, February 7, 2015 9:04 AM > Subject: Re: [Histonet] test message > To: Michelle > Cc: > > > I think it is an anti-spam message. Asking you to add yourself to their list of 'safe' addresses. Is anyone on this list able to ask them to try to make it stop? Thanks!mills> On Feb 7, 2015, at 6:43 AM, Michelle wrote:> > It seems every time I reply to a histonet email, it is followed up with a> foreign character email. I have no idea why this could be happening, if, in> fact it is related. I am just testing to see if a newly composed email will> do the same thing? > > > > Thanks,> > Michelle> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Sat Feb 7 10:32:57 2015 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Sat Feb 7 10:33:08 2015 Subject: [Histonet] RE: Hyde Away products In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC90150543665@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC90150543665@CVMMB02.cvm.tamu.edu> Message-ID: We first use a pH strip to test neutrality and then use a test kit to test for formaldehyde. We get this from Fisher and the number is HC426043. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Friday, February 06, 2015 1:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hyde Away products Does anyone uses Formalin Neutralizer Hyde Away from Fisher?. Do you do any test to figure out if is ready?, all neutralize? Thank you very much. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abtdhu <@t> gmail.com Sat Feb 7 13:16:58 2015 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Sat Feb 7 13:17:04 2015 Subject: [Histonet] TRAP staining for ffpe sections Message-ID: Hi, I make staining solution for TRAP staining. Used once Sigma kit, but unsatisfactory. You may Google for TRAP protocol online. It must be there and easier than the kit. Dorothy Hu Manager of histology and histomorphometry operation HSDM Sent from my iPad From abtdhu <@t> gmail.com Sat Feb 7 13:26:28 2015 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Sat Feb 7 13:26:31 2015 Subject: [Histonet] decalcifying sheep knee joints Message-ID: <3C2AD062-414B-4BCF-A57C-BC33A26036C9@gmail.com> Hi, You may use formic acid instead of EDTA, it will be much fast and give you sharp image of Safranin O. You can also do IHC. Dorothy Hu HSDM Sent from my iPad From abtdhu <@t> gmail.com Sat Feb 7 13:34:13 2015 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Sat Feb 7 13:34:14 2015 Subject: [Histonet] Cryosectioning undecal none Message-ID: Hi, We are using Kawamoto's Section-Lab Co. Ltd. products now. It is better than cryojane. Check Kawamoto's paper online to get protocol. Good luck. Dorothy Hu HSDM Sent from my iPad From abtdhu <@t> gmail.com Sat Feb 7 13:37:24 2015 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Sat Feb 7 13:37:25 2015 Subject: [Histonet] Why when I submit my answer to the list an email with Korea language pop up? Message-ID: Can it be blocked? Thanks. Dorothy Sent from my iPad From histotech <@t> imagesbyhopper.com Sat Feb 7 16:01:26 2015 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Sat Feb 7 16:01:40 2015 Subject: [Histonet] Frozen Sections In-Reply-To: <20150206.223723.14830.0@webmail02.vgs.untd.com> References: <20150206.223723.14830.0@webmail02.vgs.untd.com> Message-ID: We provide assistance, primarily on the outpatient frozens, but help with in-patient ones as well. What makes you ask? Michelle Sent from my iPhone On Feb 6, 2015, at 10:37 PM, "lsmallwo@juno.com" wrote: Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Feb 7 16:12:59 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Sat Feb 7 16:13:04 2015 Subject: [Histonet] it happened again - the foreign reply In-Reply-To: <00d101d042e6$f14cfc10$d3e6f430$@imagesbyhopper.com> References: <00d101d042e6$f14cfc10$d3e6f430$@imagesbyhopper.com> Message-ID: I get the same thing everytime. No, I don't know why it appears. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Histotech@imagesbyhopper.com > To: Histonet@lists.utsouthwestern.edu > Date: Sat, 7 Feb 2015 10:01:24 -0500 > CC: > Subject: [Histonet] it happened again - the foreign reply > > Does anyone have a clue as to why I keep getting a "foreign" reply to every > email I send to histonet? Is the group seeing the same foreign message I am > seeing? Am I the only one getting this message? > > > > Michelle > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Feb 7 16:39:58 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Sat Feb 7 16:40:03 2015 Subject: [Histonet] CLIA Inspection Question In-Reply-To: <00c701d042e2$a2988110$e7c98330$@imagesbyhopper.com> References: <00c701d042e2$a2988110$e7c98330$@imagesbyhopper.com> Message-ID: Probably depends on your environment or organization, but personally I would go for CAP if you are doing pathology. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Histotech@imagesbyhopper.com > To: bszpunar@umail.iu.edu; histonet@lists.utsouthwestern.edu > Date: Sat, 7 Feb 2015 09:30:34 -0500 > Subject: RE: [Histonet] CLIA Inspection Question > CC: > > Hi Histonetters, > > It's me again! ;) I am interested in your thoughts on this: would it be better to simply apply for a CAP accreditation and get both the CAP and CLIA certificates at the same time? My thoughts are these: if meeting the CAP guidelines is effectively meeting the CLIA requirements, would it make more sense to prep for one bird and get two at the same time? Would I still need to look at the CLIA regs, under this scenario? > > Out of my element, but definitely trying to learn! > > Thanks for the input I have received so far ... you all are a wonderful resource. :) > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf <@t> gmail.com Sat Feb 7 22:09:48 2015 From: garreyf <@t> gmail.com (Garreyf) Date: Sat Feb 7 22:09:53 2015 Subject: [Histonet] CLIA Inspection Question In-Reply-To: References: <00c701d042e2$a2988110$e7c98330$@imagesbyhopper.com> Message-ID: <76BE599B-F46F-4982-9DE6-F675CFF96534@gmail.com> I believe you apply for a clia certificate regardless. Then, every two years you need to be inspected. You can choose cap or joint commission to survey you. Cap meets or exceeds clia regulations. I'm more familiar with CAP. If you choose cap your lab will be required to inspect another lab. Hope this helps. Garrey Sent from my iPhone > On Feb 7, 2015, at 5:39 PM, Joelle Weaver wrote: > > Probably depends on your environment or organization, but personally I would go for CAP if you are doing pathology. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > >> From: Histotech@imagesbyhopper.com >> To: bszpunar@umail.iu.edu; histonet@lists.utsouthwestern.edu >> Date: Sat, 7 Feb 2015 09:30:34 -0500 >> Subject: RE: [Histonet] CLIA Inspection Question >> CC: >> >> Hi Histonetters, >> >> It's me again! ;) I am interested in your thoughts on this: would it be better to simply apply for a CAP accreditation and get both the CAP and CLIA certificates at the same time? My thoughts are these: if meeting the CAP guidelines is effectively meeting the CLIA requirements, would it make more sense to prep for one bird and get two at the same time? Would I still need to look at the CLIA regs, under this scenario? >> >> Out of my element, but definitely trying to learn! >> >> Thanks for the input I have received so far ... you all are a wonderful resource. :) >> >> Michelle >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sun Feb 8 08:40:27 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Feb 8 08:40:31 2015 Subject: [Histonet] Re: Frozen Sections Message-ID: Lorraine asks: >>Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist?<< This has to do more with the prestige of the pathologist(s) in the laboratory than with the actual need. Even if I'm doing the frozen section myself, I appreciate having a second person to stain the slide - it considerably improves turnaround if I'm having trouble cutting the section. Many younger pathologists do not have adequate skills in cutting frozen sections, and really need somebody to cut the sections. I cut hundreds of them during my residency, and thousands during my research fellowship, but not every pathologist has this experience. On a different topic, most formaldehyde neutralizers are simply sodium bisulfite (a.k.a. metabisulfite), but you have to have the cachet of an expensive brand-name to keep the inspectors and managers happy. Bob Richmond Samurai Pathologist Maryville TN From madary <@t> verizon.net Sun Feb 8 12:47:11 2015 From: madary <@t> verizon.net (=?utf-8?B?bWFkYXJ5QHZlcml6b24ubmV0?=) Date: Sun Feb 8 12:47:21 2015 Subject: [Histonet] Trap Message-ID: <000f4242.23032ed44abbf5a0@verizon.net> It has been a while but the best trap staining will occurr eith cold fixation and cold edta from my exp. Sent from my Verizon 4G LTE Tablet From mpence <@t> grhs.net Mon Feb 9 08:04:57 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Feb 9 08:05:21 2015 Subject: [Histonet] Frozen Sections In-Reply-To: <20150206.223723.14830.0@webmail02.vgs.untd.com> References: <20150206.223723.14830.0@webmail02.vgs.untd.com> Message-ID: We provide assistance while the dept. is staff with histology techs. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lsmallwo@juno.com Sent: Friday, February 06, 2015 9:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Sections Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Feb 9 08:07:09 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Mon Feb 9 08:07:15 2015 Subject: [Histonet] Frozen Sections In-Reply-To: References: <20150206.223723.14830.0@webmail02.vgs.untd.com>, Message-ID: same Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mpence@grhs.net > To: lsmallwo@juno.com; histonet@lists.utsouthwestern.edu > Date: Mon, 9 Feb 2015 14:04:57 +0000 > Subject: RE: [Histonet] Frozen Sections > CC: > > We provide assistance while the dept. is staff with histology techs. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lsmallwo@juno.com > Sent: Friday, February 06, 2015 9:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Frozen Sections > > Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! > Lorraine > > ____________________________________________________________ > Fast, Secure, NetZero 4G Mobile Broadband. Try it. > http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> pathlab.us Mon Feb 9 08:13:58 2015 From: histo <@t> pathlab.us (Histology) Date: Mon Feb 9 08:17:31 2015 Subject: [Histonet] fatty/ breast processing Message-ID: <566E7795BC94204D9A21539DE282AED726169CFF@DC01.dp.local> Hi All, Would anyone care to share their fatty/ breast case processing program? We are having trouble getting them fixed right. I don't need tons of detail (mainly times). Thanks in advance, Mehndi Helgren HT From Wanda.Smith <@t> HCAhealthcare.com Mon Feb 9 08:21:20 2015 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Feb 9 08:21:29 2015 Subject: [Histonet] Frozen Sections In-Reply-To: <20150206.223723.14830.0@webmail02.vgs.untd.com> References: <20150206.223723.14830.0@webmail02.vgs.untd.com> Message-ID: <4A1B8A80249114499C2812ADCDA7250902D24D@XRDCWPMSGHCMD1A.hca.corpad.net> We have 4 Pathologist and all four cut their own FS's. Two will stain and coverslip them themselves and two request assistance with staining and coverslipping. We label and set-up the cassette and slides. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lsmallwo@juno.com Sent: Friday, February 06, 2015 10:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Sections Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Mon Feb 9 09:02:44 2015 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Feb 9 09:06:29 2015 Subject: [Histonet] Frozen Sections In-Reply-To: <4A1B8A80249114499C2812ADCDA7250902D24D@XRDCWPMSGHCMD1A.hca.corpad.net> References: <20150206.223723.14830.0@webmail02.vgs.untd.com> <4A1B8A80249114499C2812ADCDA7250902D24D@XRDCWPMSGHCMD1A.hca.corpad.net> Message-ID: <450B7A81EDA0C54E97C53D60F00776C32337384209@isexstore03> As long as there is a Histotech on duty we cut, stain and coverslip all the frozens, after hours the Pathologists do their own. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Monday, February 09, 2015 9:21 AM To: lsmallwo@juno.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections We have 4 Pathologist and all four cut their own FS's. Two will stain and coverslip them themselves and two request assistance with staining and coverslipping. We label and set-up the cassette and slides. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lsmallwo@juno.com Sent: Friday, February 06, 2015 10:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Sections Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From akbitting <@t> geisinger.edu Mon Feb 9 09:10:23 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Mon Feb 9 09:10:37 2015 Subject: [Histonet] Ventana's MDM2 probe Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7F215B@GHSEXMBX1W8K1V.geisinger.edu> I'm looking for another institution who is running Ventana's MDM2 DNP probe. If you would be so kind as to contact me. Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From Ronald.Houston <@t> nationwidechildrens.org Mon Feb 9 09:51:23 2015 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Feb 9 09:53:34 2015 Subject: [Histonet] Frozen Sections In-Reply-To: References: <20150206.223723.14830.0@webmail02.vgs.untd.com> Message-ID: we do, during the day Monday - Friday (8:00-17:30) but not over the weekend -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, February 09, 2015 9:05 AM To: 'lsmallwo@juno.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections We provide assistance while the dept. is staff with histology techs. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lsmallwo@juno.com Sent: Friday, February 06, 2015 9:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Sections Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. https://urldefense.proofpoint.com/v2/url?u=http-3A__www.netzero.net_-3Frefcd-3DNZINTISP0512T4GOUT2&d=AwIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=2_hRIZxzXwfjq_7n-rmkUtP_J0qhtP0UYbxTbT_x_q4&s=yq4UN13f5ayvWibRncCpaEILkARO2iTLjQcW_sJfviY&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=2_hRIZxzXwfjq_7n-rmkUtP_J0qhtP0UYbxTbT_x_q4&s=mzJN8ccgeH4uGlJSjJQltsTHLRKZ1TfJKYCwm7H42n8&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=2_hRIZxzXwfjq_7n-rmkUtP_J0qhtP0UYbxTbT_x_q4&s=mzJN8ccgeH4uGlJSjJQltsTHLRKZ1TfJKYCwm7H42n8&e= From Toni.Rathborne <@t> rwjuh.edu Mon Feb 9 10:01:51 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Mon Feb 9 10:02:06 2015 Subject: [Histonet] Frozen Sections In-Reply-To: References: <20150206.223723.14830.0@webmail02.vgs.untd.com> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F44E12@vap1014.win.rwjuh.edu> We will provide assistance weekdays with staff that accession and stain the case. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Monday, February 09, 2015 10:51 AM To: Mike Pence; 'lsmallwo@juno.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections we do, during the day Monday - Friday (8:00-17:30) but not over the weekend -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, February 09, 2015 9:05 AM To: 'lsmallwo@juno.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections We provide assistance while the dept. is staff with histology techs. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lsmallwo@juno.com Sent: Friday, February 06, 2015 9:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Sections Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. https://urldefense.proofpoint.com/v2/url?u=http-3A__www.netzero.net_-3Frefcd-3DNZINTISP0512T4GOUT2&d=AwIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=2_hRIZxzXwfjq_7n-rmkUtP_J0qhtP0UYbxTbT_x_q4&s=yq4UN13f5ayvWibRncCpaEILkARO2iTLjQcW_sJfviY&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=2_hRIZxzXwfjq_7n-rmkUtP_J0qhtP0UYbxTbT_x_q4&s=mzJN8ccgeH4uGlJSjJQltsTHLRKZ1TfJKYCwm7H42n8&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=2_hRIZxzXwfjq_7n-rmkUtP_J0qhtP0UYbxTbT_x_q4&s=mzJN8ccgeH4uGlJSjJQltsTHLRKZ1TfJKYCwm7H42n8&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Mon Feb 9 11:35:24 2015 From: DSiena <@t> statlab.com (Debra Siena) Date: Mon Feb 9 11:35:32 2015 Subject: [Histonet] Requirements that tissue cassette storage cabinets be off floor Message-ID: Hi Histonetters- Can anyone tell me if it is a JCAHO, CAP or CLIA requirement that tissue cassette storage cabinets be at least 6 inches off the floor? Also, If possible, could you tell me how I could find this informaiton? Any information is appreciated, thanks for your help in advance. Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t: 800.442.3573 ext. 229 | f: 972.767.3992 dsiena@statlab.com | www.statlab.com From TanyaAbbott <@t> catholichealth.net Mon Feb 9 11:49:48 2015 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Feb 9 11:51:07 2015 Subject: [Histonet] Receipt of specimens Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CDA853A@CHIEX005.CHI.catholichealth.net> I am curious as to how everyone handles the receipt of their surgical, and Cytology specimens in to the lab? Especially on the off shift hours? Other clinical lab specimens are received directly in the computer in Specimen Processing when they come in the door from remote locations or via the tube system, then delivered to departments. During the day, specimens get dropped off directly to Pathology by the various departments collecting (ie. OR, Radiology, etc). On our off shifts, specimens get received by Specimen Processing(date/time stamped and initialed) and put in the fridge for Histo/Cyto the next day. When Histo and Cyto put the specimens in the computer, for the "receive date", they go by the date the specimens are "received" (or unpacked) in Histo/Cyto. I am thinking we should go by the date/time Specimen Processing received them in to the actually laboratory where the specimens are then "controlled". Any input is appreciated! Thanks! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From tpodawiltz <@t> lrgh.org Mon Feb 9 12:04:08 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Feb 9 12:04:21 2015 Subject: [Histonet] RE: Receipt of specimens In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CDA853A@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CDA853A@CHIEX005.CHI.catholichealth.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386326162EF2D7@LRGHEXVS1.practice.lrgh.org> What we do now is all specimens other than tissue for frozen section are received by our specimen processing area. Everything is date/time stamped in when they receive it and that is the date that is used on order entry. Only unfixed specimens will be refrigerated. Fixed specimens are left at room temp. Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Monday, February 09, 2015 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Receipt of specimens I am curious as to how everyone handles the receipt of their surgical, and Cytology specimens in to the lab? Especially on the off shift hours? Other clinical lab specimens are received directly in the computer in Specimen Processing when they come in the door from remote locations or via the tube system, then delivered to departments. During the day, specimens get dropped off directly to Pathology by the various departments collecting (ie. OR, Radiology, etc). On our off shifts, specimens get received by Specimen Processing(date/time stamped and initialed) and put in the fridge for Histo/Cyto the next day. When Histo and Cyto put the specimens in the computer, for the "receive date", they go by the date the specimens are "received" (or unpacked) in Histo/Cyto. I am thinking we should go by the date/time Specimen Processing received them in to the actually laboratory where the specimens are then "controlled". Any input is appreciated! Thanks! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Timothy.Morken <@t> ucsf.edu Mon Feb 9 12:03:06 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Mon Feb 9 12:06:24 2015 Subject: [Histonet] RE: Receipt of specimens In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CDA853A@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CDA853A@CHIEX005.CHI.catholichealth.net> Message-ID: <761E2B5697F795489C8710BCC72141FF367F23AA@ex07.net.ucsf.edu> Tanya, in our Copath system we have field for Date Taken (ie,service Date) and Date Received (in Pathology). So we account for items received during off hours. Or puts holds fresh specimens in a fridge for pickup, or takes to a Cyto fridge in some locations. Cyto specimens delivered after regular cytology hours are taken out of courier containers and put in the cyto fridge by Histo staff. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Monday, February 09, 2015 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Receipt of specimens I am curious as to how everyone handles the receipt of their surgical, and Cytology specimens in to the lab? Especially on the off shift hours? Other clinical lab specimens are received directly in the computer in Specimen Processing when they come in the door from remote locations or via the tube system, then delivered to departments. During the day, specimens get dropped off directly to Pathology by the various departments collecting (ie. OR, Radiology, etc). On our off shifts, specimens get received by Specimen Processing(date/time stamped and initialed) and put in the fridge for Histo/Cyto the next day. When Histo and Cyto put the specimens in the computer, for the "receive date", they go by the date the specimens are "received" (or unpacked) in Histo/Cyto. I am thinking we should go by the date/time Specimen Processing received them in to the actually laboratory where the specimens are then "controlled". Any input is appreciated! Thanks! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sheila.Tapper <@t> EssentiaHealth.org Mon Feb 9 12:30:08 2015 From: Sheila.Tapper <@t> EssentiaHealth.org (Tapper, Sheila J.) Date: Mon Feb 9 12:30:26 2015 Subject: [Histonet] Requirements that tissue cassette storage cabinets be off floor In-Reply-To: References: Message-ID: In my experience, this is usually a local fire code requirement. Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Monday, February 09, 2015 11:35 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Requirements that tissue cassette storage cabinets be off floor Hi Histonetters- Can anyone tell me if it is a JCAHO, CAP or CLIA requirement that tissue cassette storage cabinets be at least 6 inches off the floor? Also, If possible, could you tell me how I could find this informaiton? Any information is appreciated, thanks for your help in advance. Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t: 800.442.3573 ext. 229 | f: 972.767.3992 dsiena@statlab.com | www.statlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pmcdavid <@t> mhg.com Mon Feb 9 13:24:37 2015 From: Pmcdavid <@t> mhg.com (Patti McDavid) Date: Mon Feb 9 13:24:50 2015 Subject: [Histonet] RE: Histonet Digest, Vol 135, Issue 9 In-Reply-To: <20150208180138.B792E82001C@hostedmail.ventech.com> References: <20150208180138.B792E82001C@hostedmail.ventech.com> Message-ID: <1F886A89F4A53345928A11203C028957DAB65CFB@JANDC600-MBN01.mhg.local> Re: Frozen Sections The histo techs at our facility help our pathologist(s) as well as our Pathologist Assistant with the cutting and staining of frozen sections. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, February 08, 2015 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. TRAP staining for ffpe sections (abtdhu@gmail.com) 2. decalcifying sheep knee joints (abtdhu@gmail.com) 3. Cryosectioning undecal none (abtdhu@gmail.com) 4. Why when I submit my answer to the list an email with Korea language pop up? (abtdhu@gmail.com) 5. Re: Frozen Sections (histotech@imagesbyhopper.com) 6. RE: it happened again - the foreign reply (Joelle Weaver) 7. RE: CLIA Inspection Question (Joelle Weaver) 8. Re: CLIA Inspection Question (Garreyf) 9. Re: Frozen Sections (Bob Richmond) ---------------------------------------------------------------------- Message: 1 Date: Sat, 7 Feb 2015 14:16:58 -0500 From: abtdhu@gmail.com Subject: [Histonet] TRAP staining for ffpe sections To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Hi, I make staining solution for TRAP staining. Used once Sigma kit, but unsatisfactory. You may Google for TRAP protocol online. It must be there and easier than the kit. Dorothy Hu Manager of histology and histomorphometry operation HSDM Sent from my iPad ------------------------------ Message: 2 Date: Sat, 7 Feb 2015 14:26:28 -0500 From: abtdhu@gmail.com Subject: [Histonet] decalcifying sheep knee joints To: "histonet@lists.utsouthwestern.edu" Message-ID: <3C2AD062-414B-4BCF-A57C-BC33A26036C9@gmail.com> Content-Type: text/plain; charset=us-ascii Hi, You may use formic acid instead of EDTA, it will be much fast and give you sharp image of Safranin O. You can also do IHC. Dorothy Hu HSDM Sent from my iPad ------------------------------ Message: 3 Date: Sat, 7 Feb 2015 14:34:13 -0500 From: abtdhu@gmail.com Subject: [Histonet] Cryosectioning undecal none To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Hi, We are using Kawamoto's Section-Lab Co. Ltd. products now. It is better than cryojane. Check Kawamoto's paper online to get protocol. Good luck. Dorothy Hu HSDM Sent from my iPad ------------------------------ Message: 4 Date: Sat, 7 Feb 2015 14:37:24 -0500 From: abtdhu@gmail.com Subject: [Histonet] Why when I submit my answer to the list an email with Korea language pop up? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Can it be blocked? Thanks. Dorothy Sent from my iPad ------------------------------ Message: 5 Date: Sat, 07 Feb 2015 17:01:26 -0500 From: "histotech@imagesbyhopper.com" Subject: Re: [Histonet] Frozen Sections To: "lsmallwo@juno.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii We provide assistance, primarily on the outpatient frozens, but help with in-patient ones as well. What makes you ask? Michelle Sent from my iPhone On Feb 6, 2015, at 10:37 PM, "lsmallwo@juno.com" wrote: Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Sat, 7 Feb 2015 22:12:59 +0000 From: Joelle Weaver Subject: RE: [Histonet] it happened again - the foreign reply To: Michelle , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I get the same thing everytime. No, I don't know why it appears. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Histotech@imagesbyhopper.com > To: Histonet@lists.utsouthwestern.edu > Date: Sat, 7 Feb 2015 10:01:24 -0500 > CC: > Subject: [Histonet] it happened again - the foreign reply > > Does anyone have a clue as to why I keep getting a "foreign" reply to > every email I send to histonet? Is the group seeing the same foreign > message I am seeing? Am I the only one getting this message? > > > > Michelle > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Sat, 7 Feb 2015 22:39:58 +0000 From: Joelle Weaver Subject: RE: [Histonet] CLIA Inspection Question To: Michelle , 'Bryan Szpunar' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Probably depends on your environment or organization, but personally I would go for CAP if you are doing pathology. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Histotech@imagesbyhopper.com > To: bszpunar@umail.iu.edu; histonet@lists.utsouthwestern.edu > Date: Sat, 7 Feb 2015 09:30:34 -0500 > Subject: RE: [Histonet] CLIA Inspection Question > CC: > > Hi Histonetters, > > It's me again! ;) I am interested in your thoughts on this: would it be better to simply apply for a CAP accreditation and get both the CAP and CLIA certificates at the same time? My thoughts are these: if meeting the CAP guidelines is effectively meeting the CLIA requirements, would it make more sense to prep for one bird and get two at the same time? Would I still need to look at the CLIA regs, under this scenario? > > Out of my element, but definitely trying to learn! > > Thanks for the input I have received so far ... you all are a > wonderful resource. :) > > Michelle > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Sat, 7 Feb 2015 23:09:48 -0500 From: Garreyf Subject: Re: [Histonet] CLIA Inspection Question To: Joelle Weaver Cc: "histonet@lists.utsouthwestern.edu" , Bryan Szpunar Message-ID: <76BE599B-F46F-4982-9DE6-F675CFF96534@gmail.com> Content-Type: text/plain; charset=us-ascii I believe you apply for a clia certificate regardless. Then, every two years you need to be inspected. You can choose cap or joint commission to survey you. Cap meets or exceeds clia regulations. I'm more familiar with CAP. If you choose cap your lab will be required to inspect another lab. Hope this helps. Garrey Sent from my iPhone > On Feb 7, 2015, at 5:39 PM, Joelle Weaver wrote: > > Probably depends on your environment or organization, but personally I would go for CAP if you are doing pathology. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > >> From: Histotech@imagesbyhopper.com >> To: bszpunar@umail.iu.edu; histonet@lists.utsouthwestern.edu >> Date: Sat, 7 Feb 2015 09:30:34 -0500 >> Subject: RE: [Histonet] CLIA Inspection Question >> CC: >> >> Hi Histonetters, >> >> It's me again! ;) I am interested in your thoughts on this: would it be better to simply apply for a CAP accreditation and get both the CAP and CLIA certificates at the same time? My thoughts are these: if meeting the CAP guidelines is effectively meeting the CLIA requirements, would it make more sense to prep for one bird and get two at the same time? Would I still need to look at the CLIA regs, under this scenario? >> >> Out of my element, but definitely trying to learn! >> >> Thanks for the input I have received so far ... you all are a >> wonderful resource. :) >> >> Michelle >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Sun, 8 Feb 2015 09:40:27 -0500 From: Bob Richmond Subject: [Histonet] Re: Frozen Sections To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Lorraine asks: >>Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist?<< This has to do more with the prestige of the pathologist(s) in the laboratory than with the actual need. Even if I'm doing the frozen section myself, I appreciate having a second person to stain the slide - it considerably improves turnaround if I'm having trouble cutting the section. Many younger pathologists do not have adequate skills in cutting frozen sections, and really need somebody to cut the sections. I cut hundreds of them during my residency, and thousands during my research fellowship, but not every pathologist has this experience. On a different topic, most formaldehyde neutralizers are simply sodium bisulfite (a.k.a. metabisulfite), but you have to have the cachet of an expensive brand-name to keep the inspectors and managers happy. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 135, Issue 9 **************************************** [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **********PRIVATE AND CONFIDENTIAL********** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. From histotech <@t> imagesbyhopper.com Mon Feb 9 14:04:12 2015 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Mon Feb 9 14:04:29 2015 Subject: [Histonet] Requirements that tissue cassette storage cabinets be off floor In-Reply-To: References: Message-ID: <75427321-09ED-4E27-8090-BE83DAA00681@imagesbyhopper.com> I agree with Sheila. At our place, it was fire code that everything be off the ground by 6"... not just blocks, but anything being stored. Michelle Sent from my iPhone On Feb 9, 2015, at 1:30 PM, Tapper, Sheila J. wrote: In my experience, this is usually a local fire code requirement. Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Monday, February 09, 2015 11:35 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Requirements that tissue cassette storage cabinets be off floor Hi Histonetters- Can anyone tell me if it is a JCAHO, CAP or CLIA requirement that tissue cassette storage cabinets be at least 6 inches off the floor? Also, If possible, could you tell me how I could find this informaiton? Any information is appreciated, thanks for your help in advance. Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t: 800.442.3573 ext. 229 | f: 972.767.3992 dsiena@statlab.com | www.statlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.deyneko <@t> gmail.com Mon Feb 9 16:27:49 2015 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Mon Feb 9 16:27:53 2015 Subject: [Histonet] Looking for a Histotech positions in Massachusetts Message-ID: Dear Histonetters, A professional histotechnologist with nearly 7 years of experience at a small pharmaceutical company is looking for a new position in Mass. I have started a lab from scratch and purchased equipment and trained coworkers on small animal necropsies and organ harvesting. I performed subsequent tissue fixation and tissue processing and paraffin/OCT embedding, I have experience suing a rotary microtome and a cryostat. I have developed biomarker testing panels using IHC, IF and in situ techniques both manually and on DAKO and Ventana autostainers. In addition, I have developed beta gal staining method for frozen tissues and performed specialty staining as well. I have implemented image analysis capabilities in our lab, Aperio and HistoQuest modules and performed image analysis with their respective softwares. If you happen to hire or know someone who is hiring, I would appreciate any leads. Thank you very much. Igor Deyneko From oneilb <@t> wvuhealthcare.com Tue Feb 10 07:36:15 2015 From: oneilb <@t> wvuhealthcare.com (O'neil, Beth) Date: Tue Feb 10 07:36:25 2015 Subject: [Histonet] Ventana protocols for ISH control probes Message-ID: <3CEB8EBCF9C7A648B9694B5696462A717263F6A4@NT-EX2.wvuhs.com> We are optimizing/validating a new Ventana Benchmark Ultra but I need protocols for the positive RNA probe (U6 DNA Probe) and the Negative control probe. Any help would be much appreciated. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC oneilb@wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From PREISZNE <@t> mail.etsu.edu Tue Feb 10 08:53:53 2015 From: PREISZNE <@t> mail.etsu.edu (Preiszner, Johanna) Date: Tue Feb 10 08:54:10 2015 Subject: [Histonet] RE: peach stone (pit) sectioning? In-Reply-To: References: Message-ID: Thanks everybody for the ideas and the expression of sympathy! Our conclusion is that it's not possible to soften up a hard fruit pit for paraffin sectioning in a standard Histology lab. We never tried celloidin embedding, so that's still a possiblility. The best approach might be a bone or mineral grinder that can produce microscopic sections of hard material. Thanks again, Hanna Preiszner ETSU/QCOM ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Preiszner, Johanna [PREISZNE@mail.etsu.edu] Sent: Tuesday, February 03, 2015 4:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] peach stone (pit) sectioning? Hi Netters, does anybody know how I could soften up a hard fruit pit for sectioning? Thanks, Hanna Preiszner ETSU/QCOM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Feb 10 09:40:40 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Feb 10 09:40:50 2015 Subject: [Histonet] CBG Recycler users Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7F5D0A@GHSEXMBX1W8K1V.geisinger.edu> Would someone who is a CGB alcohol recycler user contact me? I can't get through to CBG. Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From dmborel <@t> aol.com Tue Feb 10 10:49:48 2015 From: dmborel <@t> aol.com (dmborel@aol.com) Date: Tue Feb 10 10:49:57 2015 Subject: [Histonet] Position listing Message-ID: <8D21368B77FB962-EC-3C5C5@webmail-va059.sysops.aol.com> A full-time histology/clericalposition is available. If currently certified as ahistotechnician or histotechnologist, the following educational requirementsare appropriately met. The applicantmust have a willingness to learn to do gross exams of specimens andhistology. Onsite training will beprovided. Sixty semester hours orequivalent from an accredited institution that, as a minimum, includes either24 hours of MLT courses or 24 hoursof science courses (6 in chemistry, 6 in biology, and 12 in chemistry, biology,or MLT in any combination) is needed. Copies of relevant transcripts must be provided so that advance approvalby our CLIA inspectors can be obtained for purposes of meeting CLIA educationrequirements to perform high complexity testing (gross tissue examination). Please contact: Jan Rice, Office Manager Pathology Services, P.A. 5650 SW 29th Street Topeka, KS 66614 (785) 272-4783 From LYDIA.BORGES <@t> Tenethealth.com Tue Feb 10 11:03:23 2015 From: LYDIA.BORGES <@t> Tenethealth.com (Borges, Lydia) Date: Tue Feb 10 11:06:26 2015 Subject: [Histonet] Lead Histotech Position in Philadelphia, PA Message-ID: <4D15461AA886234183524E2FDE396F2893D8B39A@TENHDCTHMB10-01.tenethealth.net> Hi Histonetters, We are currently looking for a full time Lead Histotech. Please apply at the St. Christopher's hospital website or send resume to me at address at bottom of this page. Performs routine and specialized Histology procedures. Including but not limited to Special Stains, Immunohistochemistry, Cytology, and Immunoflourescence. Acts under direction of the Supervisor or Director to organize and direct the daily activity of the Histology (Pathology) Laboratory, including work station coverage, quality assurance, quality control, organization of records, instrumentation operation and technical problem solving activities. Works in coordination with the supervisor to assure delivery of accurate and timely laboratory services that meet the needs of our customers in a cost effective manner. In the absence of the Section Supervisor the Lead Technologist will assume responsibility for assigned management tasks, the daily operation of the section, technical supervision and scheduling of section employees. Training & Education: An earned associate degree in a laboratory science or medical laboratory technology obtained from an accredited institution or Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), HTL(ASCP) or HT (ASCP) Certification is required. Meets CLIA - 88 qualifications and requirements for high complexity testing. Experience: Five years of clinical laboratory experience as a Histotechnologist in the technical specialty is required. Supervisory Responsibility: In the absence of the supervisor functions as the technologist in charge. Assists in development and competency assessment of staff Lydia E. Borges Histology Supervisor Department of Pathology and Laboratory Medicine St. Christopher's Hospital for Children 160 E. Erie Ave. Philadelphia, PA 19134 Tel. (215) 427-4872 Fax. (215) 427-4284 lydia.borges@tenethealth.com Compliance Disclaimer: The information in this communication is confidential and is directed only to the intended recipient. Please do not forward this communication without my permission. If you have received this communication in error, please notify me immediately and delete/destroy this communication. From JRobinson <@t> pathology-associates.com Tue Feb 10 11:27:52 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Tue Feb 10 11:28:06 2015 Subject: [Histonet] Frozen Sections In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C32337384209@isexstore03> References: <20150206.223723.14830.0@webmail02.vgs.untd.com> <4A1B8A80249114499C2812ADCDA7250902D24D@XRDCWPMSGHCMD1A.hca.corpad.net> <450B7A81EDA0C54E97C53D60F00776C32337384209@isexstore03> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8C1AD@PAEXCH1.PathologyAssociates.local> I worked in a hospital Histology Department years ago and the histotechs did everything except orient the specimen and read the slide. The group I work for now perform all of their own frozens. I still cut ORO controls on the cryostat but that's about it. I do have some concerns that the younger histo staff members have ZERO experience with frozens. It's a problem if a pathologist does ask for some assistance when there is an issue (especially with troubleshooting a cryostat). Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, February 09, 2015 7:03 AM To: 'Wanda.Smith@HCAhealthcare.com'; lsmallwo@juno.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections As long as there is a Histotech on duty we cut, stain and coverslip all the frozens, after hours the Pathologists do their own. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Monday, February 09, 2015 9:21 AM To: lsmallwo@juno.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen Sections We have 4 Pathologist and all four cut their own FS's. Two will stain and coverslip them themselves and two request assistance with staining and coverslipping. We label and set-up the cassette and slides. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of lsmallwo@juno.com Sent: Friday, February 06, 2015 10:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Sections Hi, I need to ask Histoland a question....How many HT departments provide assistance to the pathologist in the performance of frozen sections (cutting and staining of slides) to be evaluated by the pathologist? Thank you very much in advance! Lorraine ____________________________________________________________ Fast, Secure, NetZero 4G Mobile Broadband. Try it. http://www.netzero.net/?refcd=NZINTISP0512T4GOUT2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From mike <@t> pathview.com Tue Feb 10 13:25:21 2015 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Feb 10 13:25:32 2015 Subject: [Histonet] Receipt of specimens In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CDA853A@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CDA853A@CHIEX005.CHI.catholichealth.net> Message-ID: <01a501d04567$50ce26a0$f26a73e0$@pathview.com> Tanya, you can probably put either date/time into the receive date/time prompt of your LIS. It just depends on how you want to use that information. For instance, perhaps you want to look at turn around times. >From the lab's perspective, the 'clock' starts when the sample is received in the lab. On the other hand, from the physician's perspective, it starts at sample collection and from the organization's perspective the clock might start when the sample was put in the fridge. If it was 'me', I'd probably put the date/time the sample was received in the lab and I'd make sure the other information was either scanned into the case via paper or somehow noted somewhere else. The reason for my thinking is that those cases received off shift are going to skew your turnaround times if that's something you measure. Bottom line, it just depends on what you're using that information for. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Monday, February 09, 2015 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Receipt of specimens I am curious as to how everyone handles the receipt of their surgical, and Cytology specimens in to the lab? Especially on the off shift hours? Other clinical lab specimens are received directly in the computer in Specimen Processing when they come in the door from remote locations or via the tube system, then delivered to departments. During the day, specimens get dropped off directly to Pathology by the various departments collecting (ie. OR, Radiology, etc). On our off shifts, specimens get received by Specimen Processing(date/time stamped and initialed) and put in the fridge for Histo/Cyto the next day. When Histo and Cyto put the specimens in the computer, for the "receive date", they go by the date the specimens are "received" (or unpacked) in Histo/Cyto. I am thinking we should go by the date/time Specimen Processing received them in to the actually laboratory where the specimens are then "controlled". Any input is appreciated! Thanks! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Feb 10 13:32:54 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Feb 10 13:33:02 2015 Subject: [Histonet] Receipt of specimens In-Reply-To: <01a501d04567$50ce26a0$f26a73e0$@pathview.com> References: <852F7D2C14FB464D80E182B15DB138AF4CDA853A@CHIEX005.CHI.catholichealth.net> <01a501d04567$50ce26a0$f26a73e0$@pathview.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3917319E767E@IBMB7Exchange.digestivespecialists.com> I actually use three different times for tracking since it means different things to different people. I track collection to sign out, received in the lab to sign out, and report ready to sign out. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Tuesday, February 10, 2015 2:25 PM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Receipt of specimens Tanya, you can probably put either date/time into the receive date/time prompt of your LIS. It just depends on how you want to use that information. For instance, perhaps you want to look at turn around times. >From the lab's perspective, the 'clock' starts when the sample is >received in the lab. On the other hand, from the physician's perspective, it starts at sample collection and from the organization's perspective the clock might start when the sample was put in the fridge. If it was 'me', I'd probably put the date/time the sample was received in the lab and I'd make sure the other information was either scanned into the case via paper or somehow noted somewhere else. The reason for my thinking is that those cases received off shift are going to skew your turnaround times if that's something you measure. Bottom line, it just depends on what you're using that information for. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Monday, February 09, 2015 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Receipt of specimens I am curious as to how everyone handles the receipt of their surgical, and Cytology specimens in to the lab? Especially on the off shift hours? Other clinical lab specimens are received directly in the computer in Specimen Processing when they come in the door from remote locations or via the tube system, then delivered to departments. During the day, specimens get dropped off directly to Pathology by the various departments collecting (ie. OR, Radiology, etc). On our off shifts, specimens get received by Specimen Processing(date/time stamped and initialed) and put in the fridge for Histo/Cyto the next day. When Histo and Cyto put the specimens in the computer, for the "receive date", they go by the date the specimens are "received" (or unpacked) in Histo/Cyto. I am thinking we should go by the date/time Specimen Processing received them in to the actually laboratory where the specimens are then "controlled". Any input is appreciated! Thanks! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kari.Breal <@t> alexian.net Tue Feb 10 14:01:30 2015 From: Kari.Breal <@t> alexian.net (Breal, Kari) Date: Tue Feb 10 13:58:55 2015 Subject: [Histonet] MICROTOMES Message-ID: I am interested in which automated microtome is the best for reducing ergonomic issues. All opinions are welcome :) Kari H. Breal, HT (ASCP) Histology Manager ABMC- 847-437-5500 x 5155 SAMC- 847-843-2000 x 6818 Kari.Breal@alexian.net CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From PAMarcum <@t> uams.edu Tue Feb 10 14:10:19 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Feb 10 14:10:32 2015 Subject: [Histonet] RE: MICROTOMES In-Reply-To: References: Message-ID: <320f41b4524345daa162419fca464c32@MAIL13M2N2.ad.uams.edu> We have six Leica RM2255 and they are great workhorses. The automated controls are flexible as they can be on either side or on top of the microtome as needed or is most comfortable. I personally like using the foot pedal for facing the blocks and I do get old fashion and cut my sections manually. I can do it automatically just like to be hands on for the final steps. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breal, Kari Sent: Tuesday, February 10, 2015 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MICROTOMES I am interested in which automated microtome is the best for reducing ergonomic issues. All opinions are welcome :) Kari H. Breal, HT (ASCP) Histology Manager ABMC- 847-437-5500 x 5155 SAMC- 847-843-2000 x 6818 Kari.Breal@alexian.net CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Linda.Prentice <@t> ucsf.edu Tue Feb 10 14:20:54 2015 From: Linda.Prentice <@t> ucsf.edu (Prentice, Linda) Date: Tue Feb 10 14:21:02 2015 Subject: [Histonet] Kawamoto Method Message-ID: I am interested in using the Kawamoto tape method but we have not found a source for the supplies. Can anyone direct me to one? Thanks in advance Linda Prentice UCSF Preventive and Restorative Dental Sciences From Ryan.Roy <@t> va.gov Tue Feb 10 14:59:58 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Tue Feb 10 15:00:48 2015 Subject: [Histonet] Contiued Ed HTL Message-ID: <15F883394EAB744E99E1C7E1B9873049017772418A9A@R04BYNMSGB1.r04.med.va.gov> Hello, Anyone have any suggestions CE courses? This is my first time working on these as I am a new HTL. Thanks in advance, Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA From akbitting <@t> geisinger.edu Wed Feb 11 06:04:29 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Wed Feb 11 06:04:33 2015 Subject: [Histonet] CBG Recycler users In-Reply-To: References: <77F52EFAB8B1694B885E277C48FCD0F69C7F5D0A@GHSEXMBX1W8K1V.geisinger.edu> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7F667E@GHSEXMBX1W8K1V.geisinger.edu> Thank you everyone for your replies and advice regarding my recycler. As always, the best information comes from my esteemed peers on Histonet :) Have a great day !! -----Original Message----- From: histotech@imagesbyhopper.com [mailto:histotech@imagesbyhopper.com] Sent: Tuesday, February 10, 2015 5:20 PM To: Bitting, Angela K. Subject: Re: [Histonet] CBG Recycler users Hi Angie, I used it for quite a while, what's up? Michelle Sent from my iPhone On Feb 10, 2015, at 10:40 AM, Bitting, Angela K. wrote: Would someone who is a CGB alcohol recycler user contact me? I can't get through to CBG. Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne <@t> rwjuh.edu Wed Feb 11 08:36:52 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Wed Feb 11 08:36:59 2015 Subject: [Histonet] Sterile slides for FNA Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4595A@vap1014.win.rwjuh.edu> Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni From mpence <@t> grhs.net Wed Feb 11 08:42:05 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Feb 11 08:42:31 2015 Subject: [Histonet] RE: Sterile slides for FNA In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4595A@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4595A@vap1014.win.rwjuh.edu> Message-ID: We make our own sterile slides. We package 2 slides per package and send to sterile processing where then sterilize them for us. We then let the radiologist smear/touch/do their thing on the slide and the needle remains sterile. You can make up 200 packets in just a few minutes and save a lot of $$. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, February 11, 2015 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterile slides for FNA Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni From Toni.Rathborne <@t> rwjuh.edu Wed Feb 11 08:45:13 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Wed Feb 11 08:45:48 2015 Subject: [Histonet] RE: Sterile slides for FNA In-Reply-To: References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4595A@vap1014.win.rwjuh.edu> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F45995@vap1014.win.rwjuh.edu> What a great resource. How does Sterile Processing package them for you? -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, February 11, 2015 9:42 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: Sterile slides for FNA We make our own sterile slides. We package 2 slides per package and send to sterile processing where then sterilize them for us. We then let the radiologist smear/touch/do their thing on the slide and the needle remains sterile. You can make up 200 packets in just a few minutes and save a lot of $$. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, February 11, 2015 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterile slides for FNA Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni From Joyce.Weems <@t> emoryhealthcare.org Wed Feb 11 08:50:14 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Feb 11 08:50:21 2015 Subject: [Histonet] RE: Sterile slides for FNA In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4595A@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4595A@vap1014.win.rwjuh.edu> Message-ID: We do... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, February 11, 2015 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterile slides for FNA Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Joyce.Weems <@t> emoryhealthcare.org Wed Feb 11 08:51:22 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Feb 11 08:52:55 2015 Subject: [Histonet] RE: Sterile slides for FNA In-Reply-To: References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4595A@vap1014.win.rwjuh.edu> Message-ID: Ours are placed in the sterile pack for the procedure. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, February 11, 2015 9:42 AM To: 'Rathborne, Toni'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Sterile slides for FNA We make our own sterile slides. We package 2 slides per package and send to sterile processing where then sterilize them for us. We then let the radiologist smear/touch/do their thing on the slide and the needle remains sterile. You can make up 200 packets in just a few minutes and save a lot of $$. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, February 11, 2015 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterile slides for FNA Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From taylor <@t> prometheushealthcare.com Wed Feb 11 10:18:40 2015 From: taylor <@t> prometheushealthcare.com (Taylor Rinaldi) Date: Wed Feb 11 10:14:06 2015 Subject: [Histonet] IHC technician needed in Modesto, CA. Message-ID: <149001d04616$6600d5e0$320281a0$@prometheushealthcare.com> A growing anatomic pathology laboratory in Modesto, CA is looking to hire a IHC technician for their Histology department. ASCP certification preferred. This is a permanent, full time opportunity offering relocation assistance. Please reach out to me for immediate consideration. Thanks so much! Taylor Rinaldi Nationwide Laboratory Recruiter Prometheus Healthcare Office (301) 693-9057 Taylor@prometheushealthcare.com From Liz.Drinkall <@t> nmhs.org Wed Feb 11 12:16:29 2015 From: Liz.Drinkall <@t> nmhs.org (Drinkall, Liz) Date: Wed Feb 11 12:16:39 2015 Subject: [Histonet] Re: Contiued Ed HTL Message-ID: Hi Ryan, There are many options for free or inexpensive continuing education opportunities. The CAP website http://www.cap.org has some if your lab participates in any surveys or HQIP. ARUP has some free webinars at http://www.aruplab.com/education/self-education. Leica offers free webinars at http://www.leicabiosystems.com/pathologyleaders/ Webinars are nice because you can watch them anytime it's convenient. I hope this helps! Liz Liz Drinkall, MS, HTL (ASCP) CM Methodist Hospital Histology Lab/Nebraska Collaborative Lab 8303 Dodge St. Omaha, NE 68114 (402)354-4572/(402)354-7974 This message and any included attachments are from Nebraska Methodist Health System and its affiliates and are intended only for the addressee. The message may contain privileged, confidential and/or proprietary information intended only for the person(s) named. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Nebraska Methodist Health System and its affiliates in Omaha, Nebraska, U.S.A at (402)354-2280. From abtdhu <@t> gmail.com Wed Feb 11 12:53:43 2015 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Wed Feb 11 12:53:50 2015 Subject: [Histonet] Kawamoto methods Message-ID: Try this http://www.section-lab.jp/. Just email them and ask how to order. Dorothy From Pmcdavid <@t> mhg.com Wed Feb 11 13:18:55 2015 From: Pmcdavid <@t> mhg.com (Patti McDavid) Date: Wed Feb 11 13:19:05 2015 Subject: [Histonet] RE: Histonet Digest, Vol 135, Issue 12 In-Reply-To: <20150211180131.7F0E682008B@hostedmail.ventech.com> References: <20150211180131.7F0E682008B@hostedmail.ventech.com> Message-ID: <1F886A89F4A53345928A11203C028957DAB66474@JANDC600-MBN01.mhg.local> RE: Microtomes We have used Microm automated microtomes for at least 15 years. Cut great and are reliable. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, February 11, 2015 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Receipt of specimens (Michael Mihalik) 2. RE: Receipt of specimens (Blazek, Linda) 3. MICROTOMES (Breal, Kari) 4. RE: MICROTOMES (Marcum, Pamela A) 5. Kawamoto Method (Prentice, Linda) 6. Contiued Ed HTL (Roy, Ryan) 7. RE: CBG Recycler users (Bitting, Angela K.) 8. Sterile slides for FNA (Rathborne, Toni) 9. RE: Sterile slides for FNA (Mike Pence) 10. RE: Sterile slides for FNA (Rathborne, Toni) 11. RE: Sterile slides for FNA (Weems, Joyce K.) 12. RE: Sterile slides for FNA (Weems, Joyce K.) 13. IHC technician needed in Modesto, CA. (Taylor Rinaldi) ---------------------------------------------------------------------- Message: 1 Date: Tue, 10 Feb 2015 11:25:21 -0800 From: "Michael Mihalik" Subject: RE: [Histonet] Receipt of specimens To: "'Abbott, Tanya'" , Message-ID: <01a501d04567$50ce26a0$f26a73e0$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" Tanya, you can probably put either date/time into the receive date/time prompt of your LIS. It just depends on how you want to use that information. For instance, perhaps you want to look at turn around times. >From the lab's perspective, the 'clock' starts when the sample is >received in the lab. On the other hand, from the physician's perspective, it starts at sample collection and from the organization's perspective the clock might start when the sample was put in the fridge. If it was 'me', I'd probably put the date/time the sample was received in the lab and I'd make sure the other information was either scanned into the case via paper or somehow noted somewhere else. The reason for my thinking is that those cases received off shift are going to skew your turnaround times if that's something you measure. Bottom line, it just depends on what you're using that information for. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Monday, February 09, 2015 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Receipt of specimens I am curious as to how everyone handles the receipt of their surgical, and Cytology specimens in to the lab? Especially on the off shift hours? Other clinical lab specimens are received directly in the computer in Specimen Processing when they come in the door from remote locations or via the tube system, then delivered to departments. During the day, specimens get dropped off directly to Pathology by the various departments collecting (ie. OR, Radiology, etc). On our off shifts, specimens get received by Specimen Processing(date/time stamped and initialed) and put in the fridge for Histo/Cyto the next day. When Histo and Cyto put the specimens in the computer, for the "receive date", they go by the date the specimens are "received" (or unpacked) in Histo/Cyto. I am thinking we should go by the date/time Specimen Processing received them in to the actually laboratory where the specimens are then "controlled". Any input is appreciated! Thanks! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 10 Feb 2015 14:32:54 -0500 From: "Blazek, Linda" Subject: RE: [Histonet] Receipt of specimens To: Michael Mihalik , "'Abbott, Tanya'" , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E3917319E767E@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="iso-8859-1" I actually use three different times for tracking since it means different things to different people. I track collection to sign out, received in the lab to sign out, and report ready to sign out. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Tuesday, February 10, 2015 2:25 PM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Receipt of specimens Tanya, you can probably put either date/time into the receive date/time prompt of your LIS. It just depends on how you want to use that information. For instance, perhaps you want to look at turn around times. >From the lab's perspective, the 'clock' starts when the sample is >received in the lab. On the other hand, from the physician's perspective, it starts at sample collection and from the organization's perspective the clock might start when the sample was put in the fridge. If it was 'me', I'd probably put the date/time the sample was received in the lab and I'd make sure the other information was either scanned into the case via paper or somehow noted somewhere else. The reason for my thinking is that those cases received off shift are going to skew your turnaround times if that's something you measure. Bottom line, it just depends on what you're using that information for. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Monday, February 09, 2015 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Receipt of specimens I am curious as to how everyone handles the receipt of their surgical, and Cytology specimens in to the lab? Especially on the off shift hours? Other clinical lab specimens are received directly in the computer in Specimen Processing when they come in the door from remote locations or via the tube system, then delivered to departments. During the day, specimens get dropped off directly to Pathology by the various departments collecting (ie. OR, Radiology, etc). On our off shifts, specimens get received by Specimen Processing(date/time stamped and initialed) and put in the fridge for Histo/Cyto the next day. When Histo and Cyto put the specimens in the computer, for the "receive date", they go by the date the specimens are "received" (or unpacked) in Histo/Cyto. I am thinking we should go by the date/time Specimen Processing received them in to the actually laboratory where the specimens are then "controlled". Any input is appreciated! Thanks! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 10 Feb 2015 20:01:30 +0000 From: "Breal, Kari" Subject: [Histonet] MICROTOMES To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am interested in which automated microtome is the best for reducing ergonomic issues. All opinions are welcome :) Kari H. Breal, HT (ASCP) Histology Manager ABMC- 847-437-5500 x 5155 SAMC- 847-843-2000 x 6818 Kari.Breal@alexian.net CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. ------------------------------ Message: 4 Date: Tue, 10 Feb 2015 20:10:19 +0000 From: "Marcum, Pamela A" Subject: [Histonet] RE: MICROTOMES To: "'Breal, Kari'" , "histonet@lists.utsouthwestern.edu" Message-ID: <320f41b4524345daa162419fca464c32@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We have six Leica RM2255 and they are great workhorses. The automated controls are flexible as they can be on either side or on top of the microtome as needed or is most comfortable. I personally like using the foot pedal for facing the blocks and I do get old fashion and cut my sections manually. I can do it automatically just like to be hands on for the final steps. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breal, Kari Sent: Tuesday, February 10, 2015 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MICROTOMES I am interested in which automated microtome is the best for reducing ergonomic issues. All opinions are welcome :) Kari H. Breal, HT (ASCP) Histology Manager ABMC- 847-437-5500 x 5155 SAMC- 847-843-2000 x 6818 Kari.Breal@alexian.net CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 5 Date: Tue, 10 Feb 2015 20:20:54 +0000 From: "Prentice, Linda" Subject: [Histonet] Kawamoto Method To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am interested in using the Kawamoto tape method but we have not found a source for the supplies. Can anyone direct me to one? Thanks in advance Linda Prentice UCSF Preventive and Restorative Dental Sciences ------------------------------ Message: 6 Date: Tue, 10 Feb 2015 15:59:58 -0500 From: "Roy, Ryan" Subject: [Histonet] Contiued Ed HTL To: "histonet@lists.utsouthwestern.edu" Message-ID: <15F883394EAB744E99E1C7E1B9873049017772418A9A@R04BYNMSGB1.r04.med.va.gov> Content-Type: text/plain; charset="us-ascii" Hello, Anyone have any suggestions CE courses? This is my first time working on these as I am a new HTL. Thanks in advance, Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA ------------------------------ Message: 7 Date: Wed, 11 Feb 2015 12:04:29 +0000 From: "Bitting, Angela K." Subject: RE: [Histonet] CBG Recycler users To: "histotech@imagesbyhopper.com" , "histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7F667E@GHSEXMBX1W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" Thank you everyone for your replies and advice regarding my recycler. As always, the best information comes from my esteemed peers on Histonet :) Have a great day !! -----Original Message----- From: histotech@imagesbyhopper.com [mailto:histotech@imagesbyhopper.com] Sent: Tuesday, February 10, 2015 5:20 PM To: Bitting, Angela K. Subject: Re: [Histonet] CBG Recycler users Hi Angie, I used it for quite a while, what's up? Michelle Sent from my iPhone On Feb 10, 2015, at 10:40 AM, Bitting, Angela K. wrote: Would someone who is a CGB alcohol recycler user contact me? I can't get through to CBG. Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 11 Feb 2015 14:36:52 +0000 From: "Rathborne, Toni" Subject: [Histonet] Sterile slides for FNA To: "histonet@lists.utsouthwestern.edu" Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F4595A@vap1014.win.rwjuh.edu> Content-Type: text/plain; charset="us-ascii" Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni ------------------------------ Message: 9 Date: Wed, 11 Feb 2015 14:42:05 +0000 From: Mike Pence Subject: [Histonet] RE: Sterile slides for FNA To: "'Rathborne, Toni'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We make our own sterile slides. We package 2 slides per package and send to sterile processing where then sterilize them for us. We then let the radiologist smear/touch/do their thing on the slide and the needle remains sterile. You can make up 200 packets in just a few minutes and save a lot of $$. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, February 11, 2015 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterile slides for FNA Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni ------------------------------ Message: 10 Date: Wed, 11 Feb 2015 14:45:13 +0000 From: "Rathborne, Toni" Subject: [Histonet] RE: Sterile slides for FNA To: 'Mike Pence' , "histonet@lists.utsouthwestern.edu" Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F45995@vap1014.win.rwjuh.edu> Content-Type: text/plain; charset="us-ascii" What a great resource. How does Sterile Processing package them for you? -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Wednesday, February 11, 2015 9:42 AM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: RE: Sterile slides for FNA We make our own sterile slides. We package 2 slides per package and send to sterile processing where then sterilize them for us. We then let the radiologist smear/touch/do their thing on the slide and the needle remains sterile. You can make up 200 packets in just a few minutes and save a lot of $$. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, February 11, 2015 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterile slides for FNA Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni ------------------------------ Message: 11 Date: Wed, 11 Feb 2015 14:50:14 +0000 From: "Weems, Joyce K." Subject: [Histonet] RE: Sterile slides for FNA To: "Rathborne, Toni" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We do... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, February 11, 2015 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterile slides for FNA Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 12 Date: Wed, 11 Feb 2015 14:51:22 +0000 From: "Weems, Joyce K." Subject: [Histonet] RE: Sterile slides for FNA To: "'Mike Pence'" , "'Rathborne, Toni'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Ours are placed in the sterile pack for the procedure. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, February 11, 2015 9:42 AM To: 'Rathborne, Toni'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Sterile slides for FNA We make our own sterile slides. We package 2 slides per package and send to sterile processing where then sterilize them for us. We then let the radiologist smear/touch/do their thing on the slide and the needle remains sterile. You can make up 200 packets in just a few minutes and save a lot of $$. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, February 11, 2015 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterile slides for FNA Good morning everyone. I was wondering if I could get some feedback about whether anyone uses sterile slides for FNAs, if you do them. While most of the radiologists transfer the core from the end of the needle onto the slide by using a scalpel to assist, we have one radiologist who prefers to place the core directly onto the slide by using the needle. This is fine, unless he needs to go back in and make another pass. If the needle has touched a slide which is not sterile, then the needle would have to be changed to prevent contamination of the site. Does anyone use sterile slides, and are these available in a small quantity so that there is no waste? Vendors are welcome to reply to me directly, unless others would like the information as well. Toni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 13 Date: Wed, 11 Feb 2015 11:18:40 -0500 From: "Taylor Rinaldi" Subject: [Histonet] IHC technician needed in Modesto, CA. To: Message-ID: <149001d04616$6600d5e0$320281a0$@prometheushealthcare.com> Content-Type: text/plain; charset="us-ascii" A growing anatomic pathology laboratory in Modesto, CA is looking to hire a IHC technician for their Histology department. ASCP certification preferred. This is a permanent, full time opportunity offering relocation assistance. Please reach out to me for immediate consideration. Thanks so much! Taylor Rinaldi Nationwide Laboratory Recruiter Prometheus Healthcare Office (301) 693-9057 Taylor@prometheushealthcare.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 135, Issue 12 ***************************************** [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **********PRIVATE AND CONFIDENTIAL********** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. From patrick.lewis <@t> seattlechildrens.org Wed Feb 11 14:45:13 2015 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Feb 11 14:45:21 2015 Subject: [Histonet] Cryostat purchasing Message-ID: <3903BE18914F4440834F0E620415FFCA3CB54FBB@PPWEXD01d.childrens.sea.kids> Hi Everyone, Can anyone recommend a good Cryostat to buy for use with Human Tissues. I'd like to look for a reasonably priced model. Also, I'd consider paying extra for a model that is designed for human tissues so it has disinfection ability or is easier to disinfect than older models. I'd also consider going with a refurbished model if the price was right. I am used to cutting on CM3000 and CM3050s which are quite old. Thanks Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jkiernan <@t> uwo.ca Thu Feb 5 15:08:39 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Feb 12 05:35:57 2015 Subject: [Histonet] RE: Cloudy Superfrost Plus Slides In-Reply-To: References: <3A862E73E3BAEF4ABCA665F7E3AF0640999FCC5A@exmb2> Message-ID: <71f0d28d435f5.54d39587@uwo.ca> On 04/02/15, "Marcum, Pamela A" wrote: > Have you checked lot numbers and expiration dates? I have only seen issues with slides that are stored in a very humid place or where the temperatures vary greatly over a year in storage. We use the ThermoFisher Colorfrost slides and have no problems here or where I lived in Pennsylvania. > > Pam Marcum > UAMS > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Aono > Sent: Wednesday, February 04, 2015 2:35 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Cloudy Superfrost Plus Slides > > Have some cloudy Fisher Superfrost plus slides. Sometimes they're cloudy even from boxes that are still in the plastic wrapping. Does the wisdom of the collective histonet think these slides are okay to use or should be tossed? Thanks! > > Michelle (Shelly) Aono > ~~~~~~~~~~~~~~~~~~~ > Research Associate II > 107B/124 Greene Hall > Auburn University, Dept of APP > Auburn, AL 36849 > (334) 844-5594 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jkiernan <@t> uwo.ca Thu Feb 5 15:28:32 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Feb 12 05:36:00 2015 Subject: [Histonet] Rat Neutrophils In-Reply-To: <71b0874942287.54d3e059@uwo.ca> References: <959202AC61AEF942968646EC66E2BE3E690EEA9A@ESPWMSGMBX08.CSMC.EDU> <7370c1661ed44.54d2f2b1@uwo.ca> <7290fb221d760.54d2f2ee@uwo.ca> <72a08e791c233.54d2f32c@uwo.ca> <72a0cd511ec89.54d2f36a@uwo.ca> <7290ac681a75d.54d2f3e4@uwo.ca> <71b0d01a19ed8.54d2f422@uwo.ca> <72f0ed571a175.54d2f461@uwo.ca> <71c0e47a1e26b.54d2f49f@uwo.ca> <73a091a31d71a.54d2f4dd@uwo.ca> <71b0b40a1a45d.54d2f557@uwo.ca> <72a0fd6d1e1af.54d2f595@uwo.ca> <7170c68b187de.54d2f5d2@uwo.ca> <71709acb19afd.54d2f610@uwo.ca> <7370dadd1a04e.54d2f74a@uwo.ca> <71d081af405a7.54d3dc1d@uwo.ca> <73e0ad2d42dcf.54d3e01b@uwo.ca> <71b0874942287.54d3e059@uwo.ca> Message-ID: <71d0f16445ecb.54d39a30@uwo.ca> Rat neutrophils are very easy to recognize without the expense of immunohistochemistry. Their nuclei are U- and O-shaped, not segmented like the nuclei of human neutrophils. They are easy to see with any common nuclear stain such as haemalum (and eosin if desired, but not too strongly). Even better is an azure-eosin (Lillie) method or a blood stain such as Giemsa. For sections of formaldehyde-fixed tissue the pH needs to be 4-5, not the 6.8 generally considered optimal for alcohol-fixed blood films. Sections ideally should be thick enough to include most of the cell and its nucleus - about 7um. John Kiernan London, Canada = = = On 04/02/15, "Swartwood, Steven J" wrote: > Hello again everyone, > > I have a researcher requesting IHC staining for Rat Neutrophils. I've found a few papers on PubMed and a few antibodies that are specific for Rat neutrophils. The tissue being used is FFPE Rat pancreas and lungs. We are specifically looking for Rat neutrophils. There was no human neutrophils injected into the Rats. > > I just wanted to see if anyone out there does this or has done this recently and what antibodies they used. A protocol would be greatly appreciated as well. So far I've found more anti-MPO antibodies, but only 1 of them has actual pictures of neutrophil staining in Rats. I was looking into Ly6G antibodies, but none really looked too spectacular. > > Any and all information is greatly appreciated. I thank all you for your time/efforts for helping me now and the previous help I've received. > > Steven Swartwood HT(ASCP) > Cedars Sinai Medical Center > steven.swartwood@cshs.org > > IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jkiernan <@t> uwo.ca Fri Feb 6 01:23:40 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Feb 12 05:36:02 2015 Subject: [Histonet] Re: ALCIAN YELLOW ALCIAN BLUE 8GX In-Reply-To: <71b0abec41e6b.54d46bf4@uwo.ca> References: <71b0f4f841f51.54d469c9@uwo.ca> <71d0cdfd43e4a.54d46a05@uwo.ca> <71d090f5472f9.54d46a44@uwo.ca> <71f08d6c45dd4.54d46a82@uwo.ca> <7360efc145e7f.54d46ac0@uwo.ca> <7360ac1842f40.54d46afe@uwo.ca> <7360dadc47dc9.54d46b3c@uwo.ca> <71b0ba3244878.54d46bb6@uwo.ca> <71b0abec41e6b.54d46bf4@uwo.ca> Message-ID: <7340b3db43c7e.54d425ac@uwo.ca> Please explain your extravagant claim, which looks like a fraudulent advertisement. Your email address billions1998@outlook.com indicates that you are a bogus company. John Kiernan London, Canada = = = On 05/02/15, "billions1998@outlook.com" wrote: > > > > > Dear Sirs, > > We are manufacturer of ALCIAN BLUE 8GX and ALCIAN YELLOW. > > Looking forward to hearing from you asap. > Kind Regards > Minggeng Wang, Ph.D/President > SUZHOU SINOERA CHEM CO., LTD. > 125 Binhe Road, Suzhou New & Hi-Tech District, 215011 China > Tel: 0086 512 68246939 > Fax: 0086 512 68258994 > Inquiries:sinoerachem@sina.cn > General Questions: billions1998@outlook.com > http://www.sinoeratech.com > http://www.sinoerachem.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From jkiernan <@t> uwo.ca Fri Feb 6 23:50:13 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Feb 12 05:36:04 2015 Subject: [Histonet] decalcifying sheep knee joints In-Reply-To: <7330d1f61055.54d5a779@uwo.ca> References: <7380e92476e.54d5a35d@uwo.ca> <73b0d2f82611.54d5a399@uwo.ca> <73e0abaf5f3.54d5a3d7@uwo.ca> <7330b325508a.54d5a415@uwo.ca> <738080c03e6d.54d5a453@uwo.ca> <7380c77d170a.54d5a491@uwo.ca> <737090aa5a4.54d5a50b@uwo.ca> <7330a096739a.54d5a549@uwo.ca> <7340b3883e7.54d5a587@uwo.ca> <7380dd26442e.54d5a5c5@uwo.ca> <7380f087e0e.54d5a604@uwo.ca> <7330a5d76958.54d5a642@uwo.ca> <7340f00c756c.54d5a680@uwo.ca> <7340d3fb639e.54d5a6be@uwo.ca> <7340d536c04.54d5a6fc@uwo.ca> <73b0f14d1ed4.54d5a73b@uwo.ca> <7330d1f61055.54d5a779@uwo.ca> Message-ID: <73c0bf9a6a58.54d56145@uwo.ca> The answer is either neutral EDTA for several weeks or in an acid for fewer weeks. It's all there in the books! There's no quick way to decalcify a big bone. Nothing is "special" about safranine O. It is a red cationic dye that attaches to tissue anions such as nucleic acids (cell nuclei) and polyanions in mucus, bacterial and plant cell walls etc. John Kiernan London, Canada = = = On 06/02/15, Fiona J Wright wrote: > Can anyone help me out with a problem Im going to have in a few months? I > will be receiving some sheep joints for processing. They will need to be > decalcified in a manner that will leave the tissue suitable for > immunohistochemistry and special stains such as safranin O. How do I go > about this? I have previously only worked with mouse bones and decalcified > for several weeks in EDTA; this has always given me good histological > results, but I fear for sheep bones this methodology would take months. > > Any advice gratefully received. > > Fiona Wright > > Dept of Infection and Immunity > University of Sheffield > K118, Medical School > Beech Hill Road > Sheffield > S10 2RX > fiona.wright@sheffield.ac.uk > 0114 271 2102 > 07769 334438 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jkiernan <@t> uwo.ca Tue Feb 10 11:53:11 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Feb 12 05:36:05 2015 Subject: [Histonet] RE: peach stone (pit) sectioning? In-Reply-To: <72d08e762ec6f.54da455c@uwo.ca> References: <732092d129848.54da42bd@uwo.ca> <73c0d87b2cfb8.54da42f9@uwo.ca> <7170e8752cb67.54da4337@uwo.ca> <72b088582fb01.54da4375@uwo.ca> <7170b7982c5c8.54da43b3@uwo.ca> <72e09dd9299ca.54da43f1@uwo.ca> <72d08e762ec6f.54da455c@uwo.ca> Message-ID: <73c0c20d2bed5.54d9ff37@uwo.ca> The book "Botanical Microtechnique and Cytochemistry" by Graeme P. Berlyn & Jerome P. Miksche (Iowas State University Press, 1976; ISBN 813802202) give a detailed account of softening wood using hydrofluoric acid - including safety precautions for this nasty stuff. Pages 73-74. A sliding microtome is recommended. This book also has a good chapter on cutting techniques for botanical materials. It is out-of-print but available 2nd-hand quite cheaply, eg http://www.amazon.com/s?ie=UTF8&page=1&rh=n%3A283155%2Cp_27%3AGraeme%20P.%3B%20Miksche%5Cc%20Jerome%20P.%20Berlyn John Kiernan London, Canada = = = On 10/02/15, "Preiszner, Johanna" wrote: > Thanks everybody for the ideas and the expression of sympathy! > > Our conclusion is that it's not possible to soften up a hard fruit pit for paraffin sectioning in a standard Histology lab. We never tried celloidin embedding, so that's still a possiblility. > > The best approach might be a bone or mineral grinder that can produce microscopic sections of hard material. > > Thanks again, > > Hanna Preiszner > ETSU/QCOM > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Preiszner, Johanna [PREISZNE@mail.etsu.edu] > Sent: Tuesday, February 03, 2015 4:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] peach stone (pit) sectioning? > > Hi Netters, > > does anybody know how I could soften up a hard fruit pit for sectioning? > > Thanks, > Hanna Preiszner > ETSU/QCOM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From abadesuyi <@t> nrh-ok.com Thu Feb 12 09:23:55 2015 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Thu Feb 12 09:24:01 2015 Subject: [Histonet] Validation of H & E on Symphony Message-ID: <04EE4F75BB5FB246ADB68D69B746044399095FC406@MAIL.nrhnt.nrh-ok.com> Hi everyone, How are you guys doing? I hope you are all doing great. Please we have just bought a Symphony H & E stainer , and I am wondering whether someone out there is using the same equipment and would like to share both the procedure and validation process with me. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From JRobinson <@t> pathology-associates.com Thu Feb 12 09:48:37 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Thu Feb 12 09:48:53 2015 Subject: [Histonet] Leica Multistainer use for routine Giemsas Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8C2DB@PAEXCH1.PathologyAssociates.local> Good Morning- I have a Leica Multistainer that I currently use only as an overflow H & E stainer. I had high hopes when we purchased the instrument that it would prove to be very useful in automating some of our Special Stains. It ended up just being easier to continue running those stains manually. I now have a request to automate (on the Multistainer) the routine Giemsas that we run for H. pylori screening (we use DiffQuik reagents). Has anyone been successful in running Giemsas on this stainer? My main concern is the slow "arm" speed and I wonder if it will be fast enough to achieve proper differentiation when running the slides down through the alcohols. Any feedback would be greatly appreciated! Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From info <@t> biopathx.com Thu Feb 12 10:05:03 2015 From: info <@t> biopathx.com (BioPathx) Date: Thu Feb 12 10:05:14 2015 Subject: [Histonet] Positive Controls for ALK-PAX5-MUM1 Message-ID: <731984744.1487747.1423757103792.open-xchange@bosoxweb04.eigbox.net> Dear Histonetters: We are pleased to add positive controls for ALK, CD30, MUM1 and PAX5 to our Online catalog. Welcome to try our control slides! Visit Biopathx.com or contact info@biopathx.com for more information. Best Regards Biopathx.com From Allison.Scott <@t> harrishealth.org Thu Feb 12 11:47:30 2015 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Thu Feb 12 11:47:39 2015 Subject: [Histonet] Peloris Processor Message-ID: Hello to all in histoland. Does anyone have a SOP on how to operate the Peloris processor? Any help will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From bcdukes <@t> lexhealth.org Thu Feb 12 12:06:13 2015 From: bcdukes <@t> lexhealth.org (Blake Taylor) Date: Thu Feb 12 12:06:24 2015 Subject: [Histonet] Retic Kit Problems Message-ID: Anyone using the Ventana benchmark special stainers encountering inconsistent staining on your Retics? Same Run one will look great the other not staining properly. Re run it and no issues. We are shaking the kit well before each use and have increased how often we decon. Any thoughts? Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From Toni.Rathborne <@t> rwjuh.edu Thu Feb 12 12:07:51 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Thu Feb 12 12:08:01 2015 Subject: [Histonet] RE: Peloris Processor In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F46162@vap1014.win.rwjuh.edu> I would contact Leica and ask. They're very good about sending electronic versions of their manuals. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, February 12, 2015 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peloris Processor Hello to all in histoland. Does anyone have a SOP on how to operate the Peloris processor? Any help will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Thu Feb 12 12:18:56 2015 From: mw <@t> personifysearch.com (Matt Ward) Date: Thu Feb 12 12:19:10 2015 Subject: [Histonet] Field Histology Specialist - IHC Message-ID: <1e6101d046f0$5e1600a0$1a4201e0$@personifysearch.com> Good morning Histonet! We are recruiting for a field based histology (IHC) technical specialist with a world leading cancer diagnostics company. We are targeting IHC professionals in the Northern California/Bay Area. This is a field based role and offers a very competitive package including: Base Salary, Bonus, Paid Vacation, Company Car, Laptop, Cell Phone, Great Benefits, etc. If this sounds interesting, please contact me directly at mw@personifysearch.com . Have a wonderful day! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com From JRobinson <@t> pathology-associates.com Thu Feb 12 12:27:51 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Thu Feb 12 12:28:00 2015 Subject: [Histonet] RE: Retic Kit Problems In-Reply-To: References: Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8C37F@PAEXCH1.PathologyAssociates.local> Yes, Blake, the Benchmark Special Stainer does a very poor job on retics, especially bone marrow retics. We got one in December, 2013, and we do not use it for retics (I still have an old Nexes). I had a Ventana guy here multiple times as they thought it was a contamination problem. He decontaminated it several times and they changed some interior filters which helped some. The problem is the heat (or lack of), not the kit. The old Nexes would heat the entire chamber with great results. The new one has the individual heating pads but the current protocol does not give it enough heat for the retic. Ventana says a software upgrade is coming out in the second quarter of this year that will hopefully fix the problem. Keep your fingers crossed! Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blake Taylor Sent: Thursday, February 12, 2015 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retic Kit Problems Anyone using the Ventana benchmark special stainers encountering inconsistent staining on your Retics? Same Run one will look great the other not staining properly. Re run it and no issues. We are shaking the kit well before each use and have increased how often we decon. Any thoughts? Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From hmyers-bird <@t> rrmc.org Thu Feb 12 13:38:50 2015 From: hmyers-bird <@t> rrmc.org (Heidi Myers-Bird) Date: Thu Feb 12 13:39:00 2015 Subject: [Histonet] Interface Cerner with Ventana BenchMark Ultra Message-ID: Does anyone have any info/experience with trying to get Cerner/Ventana interfaced? Heidi Bird, HT (ASCP) Histotechnologist Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1791 Fax:802.747.6525 email: hmyers-bird@rrmc.org Our Vision: To be the Best Community Hospital and Health System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From hmyers-bird <@t> rrmc.org Thu Feb 12 13:46:04 2015 From: hmyers-bird <@t> rrmc.org (Heidi Myers-Bird) Date: Thu Feb 12 13:46:11 2015 Subject: [Histonet] RE: Interface Cerner with Ventana BenchMark Ultra In-Reply-To: References: Message-ID: Just to add...it's Cerner Millennium -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heidi Myers-Bird Sent: Thursday, February 12, 2015 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interface Cerner with Ventana BenchMark Ultra Does anyone have any info/experience with trying to get Cerner/Ventana interfaced? Heidi Bird, HT (ASCP) Histotechnologist Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1791 Fax:802.747.6525 email: hmyers-bird@rrmc.org Our Vision: To be the Best Community Hospital and Health System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From BZIMMERM <@t> gru.edu Thu Feb 12 14:07:15 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Feb 12 14:07:23 2015 Subject: [Histonet] HISTOPALOOZA April 17th - April 19th Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8F9F0F@EX-MLB-03.ad.georgiahealth.edu> It's that time of the year again!! Time to renew your GSH membership, register, and secure a room at the Legacy Lodge. The Legacy Lodge is located at the beautiful Lake Lanier Islands, Buford, Georgia. Having seen the Lodge firsthand, I know you won't be disappointed. It's a gorgeous facility surrounded by beautiful scenery. So, you can get your continuing education credits and enjoy a nice peaceful weekend away from the rat race. But if you want to race, try the zip line there. Looks like a lot of fun. Use the link below to access membership, the Histopalooza program, as well as the link for the lodge. http://www.histosearch.com/gsh/symposium.html From bcooper <@t> chla.usc.edu Thu Feb 12 17:27:49 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Feb 12 17:28:00 2015 Subject: [Histonet] Frozen tissue/OCT Vials Message-ID: It's almost Friday Histonetters! What kinds of containers are you storing your OCT embedded samples in? We're currently using snap top vials for all of our frozen samples that have a tendency to pop their lids when removed from our LN2 freezers! Wondering if there are some type of screw top vials that will suit our purposes. I know we will likely have to change the boxes we store our vials in as well, but that's par for the course. Any help will be greatly appreciated! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From craigak12 <@t> gmail.com Thu Feb 12 20:29:20 2015 From: craigak12 <@t> gmail.com (Jb) Date: Thu Feb 12 20:29:32 2015 Subject: [Histonet] IHC Message-ID: <8484FD07-0484-4A32-8509-19C290EA891A@gmail.com> I am new to IHC, can anyone explain an easy way to calculate the equation of IHC cost per slide? Thank you for your help... Sincerely, Craig Sent from my iPhone From j.benavides <@t> eae.csic.es Fri Feb 13 03:26:20 2015 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Fri Feb 13 03:31:29 2015 Subject: [Histonet] Question regarding dehydration Message-ID: <54DDC33C.4080203@eae.csic.es> Hi there, we are having problems trying to cut some embedded samples (they crumble in the bath and the few cuts we manage to get into HE are crap). These are formalin fixed samples (bovine foetal and placenta samples) which went straight from formlin into 100? ethanol for the dehydration before clearing. I was wondering if such drastic dehydration step (no 60?, 70? or 90? ethanol before the 100?) could have damage the tissue. Has anyone have a similar issue before? do you think the samples are ruined for histology? Thanks a lot for your help Regards Julio Instituto de Ganaderia de Monta?a Spain From TGoins <@t> mt.gov Fri Feb 13 08:47:13 2015 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Feb 13 08:47:23 2015 Subject: [Histonet] IHC In-Reply-To: <8484FD07-0484-4A32-8509-19C290EA891A@gmail.com> References: <8484FD07-0484-4A32-8509-19C290EA891A@gmail.com> Message-ID: [Cost of the reagent / (Volume of stock reagent / volume used per slide)]. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, February 12, 2015 7:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC I am new to IHC, can anyone explain an easy way to calculate the equation of IHC cost per slide? Thank you for your help... Sincerely, Craig Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides <@t> eae.csic.es Fri Feb 13 09:19:19 2015 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Fri Feb 13 09:24:29 2015 Subject: [Histonet] Question regarding dehydration In-Reply-To: <15F883394EAB744E99E1C7E1B9873049017772418AA8@R04BYNMSGB1.r04.med.va.gov> References: <15F883394EAB744E99E1C7E1B9873049017772418AA8@R04BYNMSGB1.r04.med.va.gov> Message-ID: <54DE15F7.4040105@eae.csic.es> Hi Ryan, thanks a lot for your thoughts. These blocks were processed elsewhere and sent to us for the cutting and staining. Tissues were dehydrated in five consecutive baths of ethanol 100%, 5 hours each (manual processing). Then, they went to xilene (three baths, 4 hours each) and paraffin (two batch, 1h30? each). Truth is that I have never seen such processing before. In our lab, with automatic processing, we begin with 60% ethanol and go to 70, then 95 and then 100 before ethanol/xylene. I was wondering if anybody has used the 100% ethanol processing before and which was the influence over tissues. Thanks Julio -------- Forwarded Message -------- Subject: RE: [EXTERNAL] [Histonet] Question regarding dehydration Date: Fri, 13 Feb 2015 09:06:47 -0500 From: Roy, Ryan To: 'Julio Benavides' If the tissue is fatty it will blow apart on the water bath. If the H and E staining is poor its probably the processing. What is the exact procedure for your process including time that the tissue was in each reagent? I would include this in a message to all of histonet as well as some may more knowledgeable than myself. Also, I would recommend using a 95% solution prior to the 100% alcohol (s) if not a 70-80% and 95%. Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julio Benavides Sent: Friday, February 13, 2015 4:26 AM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Question regarding dehydration Hi there, we are having problems trying to cut some embedded samples (they crumble in the bath and the few cuts we manage to get into HE are crap). These are formalin fixed samples (bovine foetal and placenta samples) which went straight from formlin into 100? ethanol for the dehydration before clearing. I was wondering if such drastic dehydration step (no 60?, 70? or 90? ethanol before the 100?) could have damage the tissue. Has anyone have a similar issue before? do you think the samples are ruined for histology? Thanks a lot for your help Regards Julio Instituto de Ganaderia de Monta?a Spain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rooki.parak <@t> gmail.com Fri Feb 13 09:31:34 2015 From: rooki.parak <@t> gmail.com (Rooki Parak) Date: Fri Feb 13 09:31:42 2015 Subject: [Histonet] Villanueva stain Message-ID: Histonetters can any one of you please share the method of preparing this stain as well the staining metho?ology. Thank you Rooki From j.benavides <@t> eae.csic.es Fri Feb 13 09:43:14 2015 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Fri Feb 13 09:48:24 2015 Subject: [EXTERNAL] RE: [Histonet] Question regarding dehydration In-Reply-To: <15F883394EAB744E99E1C7E1B9873049017772418AAA@R04BYNMSGB1.r04.med.va.gov> References: <15F883394EAB744E99E1C7E1B9873049017772418AA8@R04BYNMSGB1.r04.med.va.gov> <54DE15F7.4040105@eae.csic.es> <15F883394EAB744E99E1C7E1B9873049017772418AAA@R04BYNMSGB1.r04.med.va.gov> Message-ID: <54DE1B92.5020607@eae.csic.es> Thanks Ryan. Tissu sections where thin, something about 2 and 3 mm. I think they were well fixed (buffered formalin) nut the problem was the dehydration. to go from formalin straight into 100% ethanol looks a bit too drastic to me. Thanks for your thoughts Julio On 13/02/2015 16:44, Roy, Ryan wrote: > Its well documented in the literature that using graded alcohols in processing is advantageous to prevent hardening of the tissue. > > How thick are the tissue section cut that are being processed? It is also well documented that sections should avoid being cut thicker than 3-4mm as this prevents the penetration the fixative as well as the other reagents. > > 4 hours in each reagent seems excessive... ask other people too since I have limited experience. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julio Benavides > Sent: Friday, February 13, 2015 10:19 AM > To: histonet@lists.utsouthwestern.edu > Subject: [EXTERNAL] RE: [Histonet] Question regarding dehydration > > Hi Ryan, > > thanks a lot for your thoughts. These blocks were processed elsewhere and sent to us for the cutting and staining. Tissues were dehydrated in five consecutive baths of ethanol 100%, 5 hours each (manual processing). Then, they went to xilene (three baths, 4 hours each) and paraffin (two batch, 1h30? each). Truth is that I have never seen such processing before. In our lab, with automatic processing, we begin with 60% ethanol and go to 70, then 95 and then 100 before ethanol/xylene. > > I was wondering if anybody has used the 100% ethanol processing before and which was the influence over tissues. > > Thanks > > Julio > > > -------- Forwarded Message -------- > Subject: RE: [EXTERNAL] [Histonet] Question regarding dehydration > Date: Fri, 13 Feb 2015 09:06:47 -0500 > From: Roy, Ryan > To: 'Julio Benavides' > > > > If the tissue is fatty it will blow apart on the water bath. If the H and E staining is poor its probably the processing. > > What is the exact procedure for your process including time that the tissue was in each reagent? I would include this in a message to all of histonet as well as some may more knowledgeable than myself. > > Also, I would recommend using a 95% solution prior to the 100% alcohol (s) if not a 70-80% and 95%. > > > Ryan Roy HTL (ASCP) > Manchester Veterans Affairs Medical Center Manchester New Hampshire > > Disclosure: The content of this email does not represent the views or opinons of the VA > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julio Benavides > Sent: Friday, February 13, 2015 4:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [EXTERNAL] [Histonet] Question regarding dehydration > > Hi there, > > we are having problems trying to cut some embedded samples (they crumble in the bath and the few cuts we manage to get into HE are crap). These are formalin fixed samples (bovine foetal and placenta samples) which went straight from formlin into 100? ethanol for the dehydration before clearing. I was wondering if such drastic dehydration step (no 60?, 70? or 90? ethanol before the 100?) could have damage the tissue. Has anyone have a similar issue before? do you think the samples are ruined for histology? > > Thanks a lot for your help > > Regards > > Julio > Instituto de Ganaderia de Monta?a > Spain > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Feb 13 10:02:15 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Feb 13 10:02:22 2015 Subject: [EXTERNAL] RE: [Histonet] Question regarding dehydration In-Reply-To: <54DE1B92.5020607@eae.csic.es> References: <15F883394EAB744E99E1C7E1B9873049017772418AA8@R04BYNMSGB1.r04.med.va.gov> <54DE15F7.4040105@eae.csic.es> <15F883394EAB744E99E1C7E1B9873049017772418AAA@R04BYNMSGB1.r04.med.va.gov> <54DE1B92.5020607@eae.csic.es> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79F17BCD@SBS2K8.premierlab.local> Julio 4 to 5 hours a station is way to long for processing samples of this size, regardless of what type of tissue or species they are from. Graduated alcohol dehydration is better. I would process samples of that size (unless it was bone, fat or skin) for no longer than 45 - 60 minutes a solution for manual processing. Here is what a typical processing cycle would look like once the tissue has been adequately fixed. 50% alcohol (you want to start here if you are working with small animal tissue such as mice and rats) 70% alcohol 80% alcohol 95% alcohol 100% alcohol 100% alcohol Xylene Xylene Paraffin Paraffin Paraffin I hope this helps, you may be able to get sections by trimming into the block and then soaking them on wet ice for some time (possibly an hour or so). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julio Benavides Sent: Friday, February 13, 2015 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [EXTERNAL] RE: [Histonet] Question regarding dehydration Thanks Ryan. Tissu sections where thin, something about 2 and 3 mm. I think they were well fixed (buffered formalin) nut the problem was the dehydration. to go from formalin straight into 100% ethanol looks a bit too drastic to me. Thanks for your thoughts Julio On 13/02/2015 16:44, Roy, Ryan wrote: > Its well documented in the literature that using graded alcohols in processing is advantageous to prevent hardening of the tissue. > > How thick are the tissue section cut that are being processed? It is also well documented that sections should avoid being cut thicker than 3-4mm as this prevents the penetration the fixative as well as the other reagents. > > 4 hours in each reagent seems excessive... ask other people too since I have limited experience. > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julio > Benavides > Sent: Friday, February 13, 2015 10:19 AM > To: histonet@lists.utsouthwestern.edu > Subject: [EXTERNAL] RE: [Histonet] Question regarding dehydration > > Hi Ryan, > > thanks a lot for your thoughts. These blocks were processed elsewhere and sent to us for the cutting and staining. Tissues were dehydrated in five consecutive baths of ethanol 100%, 5 hours each (manual processing). Then, they went to xilene (three baths, 4 hours each) and paraffin (two batch, 1h30? each). Truth is that I have never seen such processing before. In our lab, with automatic processing, we begin with 60% ethanol and go to 70, then 95 and then 100 before ethanol/xylene. > > I was wondering if anybody has used the 100% ethanol processing before and which was the influence over tissues. > > Thanks > > Julio > > > -------- Forwarded Message -------- > Subject: RE: [EXTERNAL] [Histonet] Question regarding dehydration > Date: Fri, 13 Feb 2015 09:06:47 -0500 > From: Roy, Ryan > To: 'Julio Benavides' > > > > If the tissue is fatty it will blow apart on the water bath. If the H and E staining is poor its probably the processing. > > What is the exact procedure for your process including time that the tissue was in each reagent? I would include this in a message to all of histonet as well as some may more knowledgeable than myself. > > Also, I would recommend using a 95% solution prior to the 100% alcohol (s) if not a 70-80% and 95%. > > > Ryan Roy HTL (ASCP) > Manchester Veterans Affairs Medical Center Manchester New Hampshire > > Disclosure: The content of this email does not represent the views or > opinons of the VA > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julio > Benavides > Sent: Friday, February 13, 2015 4:26 AM > To: histonet@lists.utsouthwestern.edu > Subject: [EXTERNAL] [Histonet] Question regarding dehydration > > Hi there, > > we are having problems trying to cut some embedded samples (they crumble in the bath and the few cuts we manage to get into HE are crap). These are formalin fixed samples (bovine foetal and placenta samples) which went straight from formlin into 100? ethanol for the dehydration before clearing. I was wondering if such drastic dehydration step (no 60?, 70? or 90? ethanol before the 100?) could have damage the tissue. Has anyone have a similar issue before? do you think the samples are ruined for histology? > > Thanks a lot for your help > > Regards > > Julio > Instituto de Ganaderia de Monta?a > Spain > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ranna0726 <@t> gmail.com Fri Feb 13 12:59:39 2015 From: ranna0726 <@t> gmail.com (Ranna Mehta) Date: Fri Feb 13 13:00:07 2015 Subject: [Histonet] Re: Frozen tissue/OCT Vials Message-ID: Hi Brian, we use Nalgene cryovials with screw top for frozen muscle. Regards Ranna > > > > > From Lisa.White3 <@t> va.gov Fri Feb 13 13:08:44 2015 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Fri Feb 13 13:09:50 2015 Subject: : [Histonet] Retic Kit Problems Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760C4784D6@VHAV09MSGA3.v09.med.va.gov> We use the Ventana Nexes and have the same issues with the Retic and the GMS kit. We have had multiple types of tissue and controls that would not stain (including bone marrow). Repeat stains have to be done when this is happening wasting time and reagent. We take the kit out with ample time to warm up, have tried doing more decon frequency (sometimes each week or more) and have not found a specific cause for the staining or lack of staining. Histology voodoo???? Classification: Internal and External Use\\Not VA Sensitive This message has been categorized by White, Lisa M. on Friday, February 13, 2015 at 2:08:44 PM in accordance with VA Handbook 6500 From BZIMMERM <@t> gru.edu Fri Feb 13 14:01:11 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Feb 13 14:01:23 2015 Subject: [Histonet] HISTOPALOOZA 2015 SECURE YOUR ROOM Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8FA4ED@EX-MLB-03.ad.georgiahealth.edu> Don't forget to pack your suit for the heated pool at the Lodge at Lake Lanier Islands. Bring your campfire songs and stories for s'mores by the fire pit. Personally, I have flashbacks when I think about s'mores by the campfire. I was in 4th grade on a Girl Scout camping trip and I had sand in my s'mores. I was too scared to tell my leader so I ate it anyway. I would have been in tip top shape if I had a gizzard, I suppose. Meet in the Bullfrog lounge for adult beverages or on the veranda with a mint julep. Come get your "geek" on at the GSH HISTOPALOOZA. We will keep the sand outta the s'mores for ya! Stay tuned for featured speakers. And, if anyone goes to see Fifty Shades drop me a line here. :) From joelleweaver <@t> hotmail.com Fri Feb 13 16:15:16 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Feb 13 16:16:26 2015 Subject: [Histonet] IHC In-Reply-To: <8484FD07-0484-4A32-8509-19C290EA891A@gmail.com> References: <8484FD07-0484-4A32-8509-19C290EA891A@gmail.com> Message-ID: need your vendor pricing for detection, ancillaries and antibody. Divide by amount applied/used per test. Add in materials costs and a labor value. Add in overhead. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: craigak12@gmail.com > Date: Thu, 12 Feb 2015 19:29:20 -0700 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC > > > I am new to IHC, can anyone explain an easy way to calculate the equation of IHC cost per slide? Thank you for your help... > > Sincerely, > > Craig > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgflem <@t> gmail.com Sun Feb 15 08:16:31 2015 From: mgflem <@t> gmail.com (Matthew Fleming) Date: Sun Feb 15 08:16:39 2015 Subject: [Histonet] Histotech needed in Milwaukee, WI Message-ID: Part-time histotech needed for independent dermatopathology lab in downtown Milwaukee, Wisconsin. Hours and salary are negotiable. If interested, please send your CV and some indication of availability (i.e., when you could work for us) to the lab director: Matthew Fleming, MD Fleming Dermatopathology mgflem@gmail.com From careerstudio <@t> bellsouth.net Sun Feb 15 13:58:49 2015 From: careerstudio <@t> bellsouth.net (Barbara Siegel) Date: Sun Feb 15 13:58:55 2015 Subject: [Histonet] Day Shift Lab Supervisor / Histology ~ Palm Beach County, FL Message-ID: <007e01d04959$d1919380$74b4ba80$@net> Our leading Palm Beach County, Florida healthcare system client is seeking Day Shift Lab Supervisor in HISTOLOGY to oversee the following: o Staffing: hiring, training, performance appraisals o Instrument maintenance; o Quality control o Budgeting ; o Procedure manuals support o Test development ; Proficiency testing, Point of care & waived testing o Appropriate maintenance of inventory of supplies & materials o Emergency & disaster codes & drills implementation o Compliance with all local, state & federal regulations o Technical / informational assistance to customers & regulatory agencies Key requirements for this role are - Bachelors Degree with science concentration - Prior solid histology laboratory exp as Technologist. - 3+ of demonstrated leadership success in high-volume histology lab - Florida State License as Supervisor of Clinical Laboratory Science in Histology Competitive starting salary & bonus eligibility are offered. For more information, please contact: June Benney Career Studio, Hospital/Lab division labcareers@aol.com 561-738-6363 www.linkedin.com/in/healthcareersusa . We have many fine hospital & clinical reference lab clients, nationwide. From davidkins <@t> yahoo.com Sun Feb 15 17:32:01 2015 From: davidkins <@t> yahoo.com (David Kinsley) Date: Sun Feb 15 17:35:04 2015 Subject: [Histonet] ATRX Ab Recommendation Message-ID: <1424043121.51454.YahooMailBasic@web163101.mail.bf1.yahoo.com> Hi, I've been asked to work up an ATRX antibody on formalin fixed human brain sections. I will be using Leica's Bond platform and detection for staining. I am looking for help with getting this up and running as soon as possible and am looking for recommendations of Abs and pretreatment conditions. I have searched the internet and biocompare, and found 29 ATRX Abs available, but I would like to narrow my options down. If anyone has had success with this antibody and is willing to share their staining conditions and the antibody they use I would be very grateful. Thanks in advance for your help. Dave From kdwyer3322 <@t> aol.com Sun Feb 15 18:00:27 2015 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Feb 15 18:07:28 2015 Subject: [Histonet] Only 5 weeks until meeting! "Texas Histology: A Wealth of Knowledge" Texas Society for Histotechnology Annual Symposium/Convention Dallas/Plano Marriott at Legacy TownCenter,Plano, Texas-March 20-22, 2015 In-Reply-To: <8D20B830DB668C2-610-5BE8@webmail-vm130.sysops.aol.com> References: <8D20B830DB668C2-610-5BE8@webmail-vm130.sysops.aol.com> Message-ID: <14b8fafa33c-1ccc-1dfb2@webprd-m87.mail.aol.com> Hi Histonetters! The Texas Society for Histotechnology will be holding the 2015 S/C in Plano Texas. If you are interested in a electronic version of the program please send me a e-mail via this communication. It is not too late to sign up. We would love to have you join us this year! Go to txsh.org. Regards, Kathy Dwyer Take a look at the great symposiums and workshop we have to offer! Take Control of Your Controls- Heather Renko Understanding the Business of Pathology and How It Affects Your AP Laboratory- Loretta Sayles The Science of Fixation and Processing- Jan Minshew H. Pylori: Special Staining Methods and Troubleshooting - Ranna Mehta/ Debbie Siena Cool Tips for Cryostat Users - Jan Minshew Resume Wriitng and Interview Preparation ? JanaE Mitchell/ Amy Plewinski Optimization of Microtomy - Past, Present and the 21st Century -H. Skip. Brown Unmasking IHC and Molecular Techniques ? Brent Hart A Pratical Approach For The Histological Evaluation of Underminerlized Bone, synthetic Biomaterial and Medical Devie Implants ? Jack Ratliff A Critical Component of Autopy Pathology ? Shemeika Johnson From Mouse to Microscope - Jennifer Johnson Understanding the Characteristics of Stains and Dyes and Their Importance in the Histology Laboratory ? Debbie Siena/ Gary Weiderhold Guidelines for Studing Special Stains in preparation for the HT Examination ? Rayan Gonzalez/Lin Bustamante Capital Budgeting - Olga Kochar Streamling the Path Line ? Kelsi Currier/Carol BethTaylor /Ary Franklin Introduction to ISH and Case Studies ? Heather Renko The Pathologist Said: "I have Achromotyfil" and You Said" What?Is That" ? Pamela Marcum Quality Management- Yesterday, Today and Tomorrow-Olga Kochar Fundamental IHC Technique and Theories of In situ Hybridization with a Comparison to Immunohistochemistry-Theresa Burchette Oh My! What Does CLIA and CAP Want During and After A Inspection ? Debbie Siena/Kathy Dwyer Technological Advancements in Microtomy:A Non-Contact Alternative to Conventional Histology Equipment and Techniques- Jack Ratliff Leadership: Theory to Application ? Jennifer Nelson From Albert.Santiago <@t> uphs.upenn.edu Mon Feb 16 09:09:17 2015 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Mon Feb 16 09:09:23 2015 Subject: [Histonet] Histology job opening Message-ID: Good morning Histonetters, We here at the University of Pennslyvania Hospital have per diem and part time temp openings available at the Dermatopathology lab. If anyone is interested please contact Albert Santiago, Lab Manager, at 215-662-6539. Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago@uphs.upenn.edu From jfinley <@t> wakehealth.edu Mon Feb 16 09:15:20 2015 From: jfinley <@t> wakehealth.edu (Joseph Finley) Date: Mon Feb 16 09:16:09 2015 Subject: [Histonet] Modified Bielschowsky Stain Message-ID: <08E3F6B222E95145BB62A542C7F1015C1683162E@EXCHDB2.medctr.ad.wfubmc.edu> Hello. Does anyone know the protocol for the Yamamato-Hirano modification of the Bielschowsky silver stain? This is possibly known as the "reformed Gros-Schultze's modification." Thanks in advance. Joseph Wayne Finley II Laboratory Technician III, HTL (ASCP)cm Department of Comparative Medicine Medical Center Blvd \ Winston-Salem, NC 27157-1040 (336) 716-1536 jfinley@wakehealth.edu From martha.davenport <@t> uky.edu Thu Feb 12 07:41:05 2015 From: martha.davenport <@t> uky.edu (Davenport, Martha R) Date: Mon Feb 16 11:08:24 2015 Subject: [Histonet] processing problem Message-ID: <52BB8F8893DC3A46BB91934E1D55977B45EDA410@ex10mb02.ad.uky.edu> I am Martha Davenport, supervisor, at the University of KY histology lab. We had a processing problem caused by accidently placing 70% (instead of 100%) into the last dehydration container. Would anyone please give me info on how you would remedy this? We usually reprocess the tissue but have had trouble in the past with the tissue morphology being optimal. Any help will be greatly appreciated. Martha Davenport 859-257-1822 From gmartin <@t> marshallmedical.org Fri Feb 13 16:34:59 2015 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Mon Feb 16 11:08:47 2015 Subject: [Histonet] Specimen release Message-ID: <6ED9D4252F278841A0593D3D788AF24C29C01906@mailsvr.MARSHMED.local> We are revisiting our policy of releasing tissue to patients. Presently we release placentas' to patients for "Religious reasons", but it seems to be escalating to include appendix, uterus, etc. I would like to understand what others are doing. Thanks in advance. Gary From christiecgowan <@t> dermatology.med.ufl.edu Thu Feb 12 13:56:25 2015 From: christiecgowan <@t> dermatology.med.ufl.edu (Gowan,Christie C) Date: Mon Feb 16 11:08:59 2015 Subject: [Histonet] RE: Interface Cerner with Ventana BenchMark Ultra In-Reply-To: References: Message-ID: Yes, tons of experience. Your Ventana people also should be a big help as they have been interfacing with Cerner Millennium for years. Christie Gowan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heidi Myers-Bird Sent: Thursday, February 12, 2015 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Interface Cerner with Ventana BenchMark Ultra Just to add...it's Cerner Millennium -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heidi Myers-Bird Sent: Thursday, February 12, 2015 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interface Cerner with Ventana BenchMark Ultra Does anyone have any info/experience with trying to get Cerner/Ventana interfaced? Heidi Bird, HT (ASCP) Histotechnologist Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1791 Fax:802.747.6525 email: hmyers-bird@rrmc.org Our Vision: To be the Best Community Hospital and Health System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peterrabbit345 <@t> verizon.net Sat Feb 14 10:38:16 2015 From: peterrabbit345 <@t> verizon.net (peterrabbit345@verizon.net) Date: Mon Feb 16 11:09:01 2015 Subject: [Histonet] tissue arrayer Message-ID: <6353448.82812.1423931896283.JavaMail.root@vznit170116.mailsrvcs.net> Does anyone know of a forum on which I can sell a single demo model of a Beecher Instruments manual tissue arrayer (MTA1)? It is in "like new" condition in original packaging and should go to a good home and not be collecting dust on my shelf. If you have a suggestion or are interested please email [1]peterrabbit345@verizon.net . Thanks. References 1. mailto:peterrabbit345@verizon.net From mills <@t> 3scan.com Mon Feb 16 11:30:41 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Mon Feb 16 11:31:08 2015 Subject: [Histonet] processing problem In-Reply-To: <52BB8F8893DC3A46BB91934E1D55977B45EDA410@ex10mb02.ad.uky.edu> References: <52BB8F8893DC3A46BB91934E1D55977B45EDA410@ex10mb02.ad.uky.edu> Message-ID: I am afraid your tissue might be 'toast'. I tried to reprocess from that same issue and things did not go well. We were processing mouse tissue, which is dry enough, but then when we put the tissues back through xylene and into 100% and back again it got even worse. We had to just get what we could from the blocks and confess to the researchers what had happened :( sorry for the bad news, I hope someone has better for you! mills On Thu, Feb 12, 2015 at 5:41 AM, Davenport, Martha R < martha.davenport@uky.edu> wrote: > I am Martha Davenport, supervisor, at the University of KY histology lab. > We had a processing problem caused by accidently placing 70% (instead of > 100%) into the last dehydration container. Would anyone please give me info > on how you would remedy this? We usually reprocess the tissue but have had > trouble in the past with the tissue morphology being optimal. Any help will > be greatly appreciated. > Martha Davenport 859-257-1822 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller Director of Histology 3Scan.com 415 2187297 From rjbuesa <@t> yahoo.com Mon Feb 16 11:35:38 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 16 11:36:18 2015 Subject: [Histonet] processing problem In-Reply-To: <52BB8F8893DC3A46BB91934E1D55977B45EDA410@ex10mb02.ad.uky.edu> References: <52BB8F8893DC3A46BB91934E1D55977B45EDA410@ex10mb02.ad.uky.edu> Message-ID: <600740838.4414291.1424108138637.JavaMail.yahoo@mail.yahoo.com> Having morphology problems when "re-processing" a tissue after an accident as you had is the "unavoidable" consequence.?The problem is rooted in the fact that usually you remove the paraffin using the tissue processor cleaning cycle and that is a very harsh method (albeit the most expeditious). If you? "re-process" using more steps than usual in the dehydration you may have very slightly better results, but not that much.The mistake you made have the consequences you fear.Ren? J.? On Monday, February 16, 2015 12:08 PM, "Davenport, Martha R" wrote: I am Martha Davenport, supervisor, at the University of KY histology lab. We had a processing problem caused? by? accidently placing 70% (instead of 100%) into the last dehydration container. Would anyone please give me info on how you would remedy this?? We usually reprocess the tissue but have had trouble in the past with the tissue morphology being optimal. Any help will be greatly appreciated. Martha Davenport 859-257-1822 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barryrittman <@t> gmail.com Mon Feb 16 12:47:45 2015 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Mon Feb 16 12:47:59 2015 Subject: [Histonet] processing problem In-Reply-To: References: <52BB8F8893DC3A46BB91934E1D55977B45EDA410@ex10mb02.ad.uky.edu> Message-ID: Hi Martha I think that Caroline is correct but you have nothing to lose by removing the wax and then trying to dehydrate and so on. A more gentle way after removing the wax and dehydrating is to use chloroform instead of xylene and hand processing. Chloroform is much more gentle although tissue do usually float in it an do not become transparent. However unlike xylene, tissue can be left in chloroform for hours to overnight without any appreciable hardening. Then after chloroform you can leave the tissues in a mixture of chloroform/wax (1:1) at room temperature again for hours to even overnight. During this process some wax does penetrate into the tissue and therefore can leave in wax for less time with less chance of hardening. Yours is certainly not the first lab that this has happened to. Good luck to you. Barry On Mon, Feb 16, 2015 at 11:30 AM, Caroline Miller wrote: > I am afraid your tissue might be 'toast'. I tried to reprocess from that > same issue and things did not go well. We were processing mouse tissue, > which is dry enough, but then when we put the tissues back through xylene > and into 100% and back again it got even worse. > > We had to just get what we could from the blocks and confess to the > researchers what had happened :( > > sorry for the bad news, I hope someone has better for you! > > mills > > On Thu, Feb 12, 2015 at 5:41 AM, Davenport, Martha R < > martha.davenport@uky.edu> wrote: > > > I am Martha Davenport, supervisor, at the University of KY histology lab. > > We had a processing problem caused by accidently placing 70% (instead > of > > 100%) into the last dehydration container. Would anyone please give me > info > > on how you would remedy this? We usually reprocess the tissue but have > had > > trouble in the past with the tissue morphology being optimal. Any help > will > > be greatly appreciated. > > Martha Davenport 859-257-1822 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From suetp918 <@t> comcast.net Mon Feb 16 14:26:42 2015 From: suetp918 <@t> comcast.net (Sue) Date: Mon Feb 16 14:27:09 2015 Subject: [Histonet] Specimen release In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C29C01906@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C29C01906@mailsvr.MARSHMED.local> Message-ID: <1141101281.975345.1424118402720.JavaMail.zimbra@comcast.net> For the most part we release nothing to patients,? all our releases are approved by our Risk Management Department..? Hardware for litigation purposes must be released to an approved storage lab.? Anything that must be buried must go through a funeral home. ? Sue Paturzo From JRobinson <@t> pathology-associates.com Tue Feb 17 10:36:04 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Tue Feb 17 10:36:18 2015 Subject: [Histonet] RE: processing problem In-Reply-To: <52BB8F8893DC3A46BB91934E1D55977B45EDA410@ex10mb02.ad.uky.edu> References: <52BB8F8893DC3A46BB91934E1D55977B45EDA410@ex10mb02.ad.uky.edu> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8C583@PAEXCH1.PathologyAssociates.local> Hi Martha- I have had some limited success in rehabilitating underprocessed tissue without reprocessing it in the tissue processor. If the tissue samples are not too large you may be able to let them air dry for a period of time so that they dry out. Make sure to face the blocks if you haven't already done so. You can then melt them down and put them in a mold with liquid paraffin and just let them sit on the embeddeder (still in liquid paraffin) for a couple of hours for better infiltration. Re-embed your samples and hope for the best. I hope this helps. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davenport, Martha R Sent: Thursday, February 12, 2015 5:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing problem I am Martha Davenport, supervisor, at the University of KY histology lab. We had a processing problem caused by accidently placing 70% (instead of 100%) into the last dehydration container. Would anyone please give me info on how you would remedy this? We usually reprocess the tissue but have had trouble in the past with the tissue morphology being optimal. Any help will be greatly appreciated. Martha Davenport 859-257-1822 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From klaus.dern44 <@t> gmail.com Tue Feb 17 11:37:11 2015 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Tue Feb 17 11:37:26 2015 Subject: [Histonet] THICK AND THIN SECTIONS ? Message-ID: If you are using one of the following microtomes and are told your thick and thin sectioning problem is caused by a worn out advance mechanism. ( too much play between spindle and spindle nut ) You could be faced with purchasing a new Microtome. ( No parts availability ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA RM 2125 RT LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. For Information, contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com From robert.jacox <@t> thermofisher.com Tue Feb 17 13:09:38 2015 From: robert.jacox <@t> thermofisher.com (Jacox, Robert A.) Date: Tue Feb 17 13:10:36 2015 Subject: [Histonet] THICK AND THIN SECTIONS ? In-Reply-To: References: Message-ID: Someone you may want to meet Sent from my iPad > On Feb 17, 2015, at 10:39 AM, Klaus Dern wrote: > > If you are using one of the following microtomes and are told your thick > and thin sectioning problem is caused by a worn out advance mechanism. ( > too much play between spindle and spindle nut ) > > You could be faced with purchasing a new Microtome. ( No parts availability > ) > > REICHERT/JUNG 2030 > LEICA RM 2125 > LEICA RM 2125 RT > LEICA 2030 Biocut > LEICA/JUNG 2035 > LEICA - CM 1850 Cryostat > SAKURA SRM 200 > > > Rather than replacing these excellent Instruments, I have a PERMANENT > solution to fix this problem. > > For Information, contact: > > Klaus Dern > Phone: 706 635-8840 > E-Mail: klaus.dern44@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Tue Feb 17 14:15:09 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Feb 17 14:15:22 2015 Subject: [Histonet] GSH HISTOPALOOZA APRIL 17- APRIL 19 Message-ID: This is a reminder to register for the Histopalooza at Lake Lanier Islands. This will be a great time to visit because the weather will be warm and enjoyable. The Lodge at Lake Lanier Islands host many events such as ours. Folks have wedding receptions here. You might decide to be a multi-tasker. Take continuing education workshops and be a "wedding crasher" afterwards. Just kidding !! **The HT/HTL review will be conducted by Vinnie Della Speranza (aka the Godfather of Histology) on Friday, April 17th. It will be an all-day class. This will be a very informative and helpful workshop for those individuals wanting to pass the certification test the first time. Vinnie is an expert in many areas of histology. He will incorporate the subject matter found on the ASCP Certification test. Who knows Vinnie has been around so long that he probably wrote the test while riding his mammoth to work. Please join us at the Georgia Society for Histotechnology meeting in April. Billie Zimmerman GSH Secretary From mpence <@t> grhs.net Tue Feb 17 14:47:51 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Feb 17 14:48:36 2015 Subject: [Histonet] Bone Saw Message-ID: I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? From jmcgough <@t> clinlab.com Tue Feb 17 14:59:03 2015 From: jmcgough <@t> clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Tue Feb 17 14:52:41 2015 Subject: [Histonet] Bone Saw In-Reply-To: References: Message-ID: We use a Dremel tool. It works great!! Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough@clinlab.com www.clinlab.com -----Original message----- > From:Mike Pence > > Sent: Tuesday, February 17, 2015 1:56 PM > To: histonet-bounces@lists.utsouthwestern.edu ; histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone Saw > > I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Toni.Rathborne <@t> rwjuh.edu Tue Feb 17 14:54:28 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Tue Feb 17 14:55:40 2015 Subject: [Histonet] Bone Saw In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F47A32@vap1014.win.rwjuh.edu> We use a device from Mopec. http://media3.mopec.com/media/pdf/AutopsyAccessories(Page76).pdf It's manual, but works great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason McGough Sent: Tuesday, February 17, 2015 3:59 PM To: Mike Pence; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone Saw We use a Dremel tool. It works great!! Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough@clinlab.com www.clinlab.com -----Original message----- > From:Mike Pence > > Sent: Tuesday, February 17, 2015 1:56 PM > To: histonet-bounces@lists.utsouthwestern.edu ; histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone Saw > > I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Feb 17 14:59:06 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Feb 17 14:59:28 2015 Subject: [Histonet] Bone Saw In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24F47A32@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F47A32@vap1014.win.rwjuh.edu> Message-ID: Those double bladed hacksaws work great for femoral heads, but are not good for toes! I also have one pathologist that wants to cut their own specimens and they do not want anything manual. They want one of those "rrrrrr" "thingies" that cuts bone. -----Original Message----- From: Rathborne, Toni [mailto:Toni.Rathborne@rwjuh.edu] Sent: Tuesday, February 17, 2015 2:55 PM To: 'Jason McGough'; Mike Pence; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone Saw We use a device from Mopec. http://media3.mopec.com/media/pdf/AutopsyAccessories(Page76).pdf It's manual, but works great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason McGough Sent: Tuesday, February 17, 2015 3:59 PM To: Mike Pence; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bone Saw We use a Dremel tool. It works great!! Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough@clinlab.com www.clinlab.com -----Original message----- > From:Mike Pence > > Sent: Tuesday, February 17, 2015 1:56 PM > To: histonet-bounces@lists.utsouthwestern.edu ; histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone Saw > > I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryeo <@t> wchosp.org Tue Feb 17 15:25:20 2015 From: ryeo <@t> wchosp.org (Richard Yeo) Date: Tue Feb 17 15:25:57 2015 Subject: [Histonet] Bone Saw In-Reply-To: References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F47A32@vap1014.win.rwjuh.edu>, Message-ID: <9892F600-E755-45EE-9679-90082EF253C7@wchosp.org> We use a small band saw from MAR-MED. cuts through a femoral head like a hot knife through soft butter. You let the blade do the job, don't force the bone. Just very little consistent pressure. Rich Y Sent from my iPhone > On Feb 17, 2015, at 4:01 PM, Mike Pence wrote: > > Those double bladed hacksaws work great for femoral heads, but are not good for toes! I also have one pathologist that wants to cut their own specimens and they do not want anything manual. They want one of those "rrrrrr" "thingies" that cuts bone. > > -----Original Message----- > From: Rathborne, Toni [mailto:Toni.Rathborne@rwjuh.edu] > Sent: Tuesday, February 17, 2015 2:55 PM > To: 'Jason McGough'; Mike Pence; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Bone Saw > > We use a device from Mopec. > > http://media3.mopec.com/media/pdf/AutopsyAccessories(Page76).pdf > > It's manual, but works great. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jason McGough > Sent: Tuesday, February 17, 2015 3:59 PM > To: Mike Pence; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Bone Saw > > We use a Dremel tool. It works great!! > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough@clinlab.com > > www.clinlab.com > > > > -----Original message----- >> From:Mike Pence > >> Sent: Tuesday, February 17, 2015 1:56 PM >> To: histonet-bounces@lists.utsouthwestern.edu ; histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Bone Saw >> >> I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Feb 17 15:47:20 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Feb 17 15:50:57 2015 Subject: [Histonet] needle size Message-ID: Does anyone have the gauge of needle used for breast core biopsies and for prostate biopsies? Thanks, Jennifer From Kimberly <@t> animalreferencepathology.com Wed Feb 18 08:20:26 2015 From: Kimberly <@t> animalreferencepathology.com (Kimberly Marshall) Date: Wed Feb 18 08:20:52 2015 Subject: [Histonet] Help with cutting mouse brain at 9-10 Microns Message-ID: <1424269230196.97641@animalreferencepathology.com> ?Hello Histo folks I am starting a research project with mouse brain, I am having trouble with chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all. I have soaked them in warm water and wonder in using a softener like conditioner would help. I am new to the animal tissue world and any advise would help. Thanks in advance. Kimberly From thisisann <@t> aol.com Wed Feb 18 08:41:50 2015 From: thisisann <@t> aol.com (Ann Specian) Date: Wed Feb 18 08:41:55 2015 Subject: [Histonet] IHC Cost Message-ID: <14b9d2349a0-75d4-27baa@webprd-m44.mail.aol.com> Does anyone know the "average" cost to perform an IHC stain? I know there is a large range, but I am looking for an average. thanks, Ann From PAMarcum <@t> uams.edu Wed Feb 18 09:01:43 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Feb 18 09:01:57 2015 Subject: [Histonet] RE: Help with cutting mouse brain at 9-10 Microns In-Reply-To: <1424269230196.97641@animalreferencepathology.com> References: <1424269230196.97641@animalreferencepathology.com> Message-ID: Please give your processing schedule with times and reagents? It is hard to help without knowing how the tissue was handled from sacrifice to embedding. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Marshall Sent: Wednesday, February 18, 2015 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help with cutting mouse brain at 9-10 Microns ?Hello Histo folks I am starting a research project with mouse brain, I am having trouble with chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all. I have soaked them in warm water and wonder in using a softener like conditioner would help. I am new to the animal tissue world and any advise would help. Thanks in advance. Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jvickroy <@t> SpringfieldClinic.com Wed Feb 18 11:27:50 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Wed Feb 18 11:28:00 2015 Subject: [Histonet] New lab setup Message-ID: <9B1A1501A800064397369BD8072E6BCA984027@E2K10DB.springfieldclinic.com> I am working on setting up an adequate validation study for tissue processing at a new lab. I want it to be adequate but also not too labor intensive or costly. I have been in Histotechnology for over 36 years and have done this before in the lab I can from. Unfortunately sometimes I make things more complicated than others. I realize the standards for testing new protocols and antibodies are defined in the CAP checklist but routine tissue processing just says to document the procedure and to run parallel samples as a blind study. We are going to run only GI biopsies at first and I had planned on running parallel samples with the department from my previous employer. I also thought that down the road we may have more than a rapid biopsy program so I should do a few larger samples at a regular processing schedule to validate both schedules (rapid and normal). I also thought that I should pick out a couple of blocks for representative special stains and IHC stains tha we would compare even though the new lab will send everything to the old lab for special stains and IHC stains. We have two processors and know I will also have to run a small validation of each new processor. I figured as I was validating the tissue processing programs I would also be validating the first tissue processor. Both of our two processors will be VIP6(s) and the machines from my former employer were also VIP6(s) which were of course validated. Can anyone share how many samples are adequate for tissue processing program validation and new processor validation? In other words what have you done and what was accepted by CAP since the wording of the question does not state any particulars? Also can you tell me if you think the special stains and IHC stains comparison is necessary, given that all of the stains will be done at the old lab and the only difference will be where the tissues were processed. My original design of the study had 25 parallel samples but I am wondering if that is overkill. Thanks Jim Vickroy Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From tbraud <@t> holyredeemer.com Wed Feb 18 12:14:43 2015 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Feb 18 12:17:03 2015 Subject: [Histonet] RE: Bone Saw In-Reply-To: <20150218165725.8C01A1EACC5@trendmess-svr.holyredeemer.local> References: <20150218165725.8C01A1EACC5@trendmess-svr.holyredeemer.local> Message-ID: We also use the little table-top band saw from Mar-Med. It has a small footprint, splash guards, and is just awesome for cutting thin beautiful sections. We use toothed forceps to hold the specimen and keep fingers out of the way. Like the man said, a hot knife through soft butter! We have 3 Pathologists and one PA and they all LOVE it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 > I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? We use a small band saw from MAR-MED. cuts through a femoral head like a hot knife through soft butter. You let the blade do the job, don't force the bone. Just very little consistent pressure. Rich Y --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Smith_D <@t> kids.wustl.edu Wed Feb 18 12:44:16 2015 From: Smith_D <@t> kids.wustl.edu (Smith, Denise) Date: Wed Feb 18 12:45:47 2015 Subject: [Histonet] RE: Help with cutting mouse brain at 9-10 Microns Message-ID: <7578651EA53E304B96B06AD6D7B8992C202BE6BD@pcfmbx01.wusm-pcf.wustl.edu> Several questions: 1. Like Pamela has asked - what's the processing schedule and what regents are used? I've had some problems if the brain is soaked in EtOH too long (70% or higher), it will cause chattering. 2. Do you slice the whole brain including cerebellum and spinal cord or did you split the hemispheres to slice them individually? 3. Since you said that you cannot get any slices more than 9-10um at all, could you describe the situation? (i.e. can't get ribbons, it was cut unevenly such as thick and thin alternatively, paraffin too hard/too soft, etc) I mostly section rat and mouse brains at 10um without any problems. What I normally do is once the paraffin block (with brain inside of it, of course) is solidified, I tend to trim around the block around where the brain is and put it directly (the brain facing towards the ice) on the ice (NOT dry ice, just normal crushed ice) for 2-3 hours before sectioning it. After being on ice and right before sectioning, I like to rub with a small soft towel smooth the edges off around the brain then quickly dip it in the warm bath then section right away. I do not know why I always do those above, but it sure helped me every time I slice any rodent brains. Maybe that will help you somehow! Denise ------------------------------ Message: 9 Date: Wed, 18 Feb 2015 14:20:26 +0000 From: Kimberly Marshall > Subject: [Histonet] Help with cutting mouse brain at 9-10 Microns To: "histonet@lists.utsouthwestern.edu" > Message-ID: <1424269230196.97641@animalreferencepathology.com> Content-Type: text/plain; charset="iso-8859-1" ?Hello Histo folks I am starting a research project with mouse brain, I am having trouble with chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all. I have soaked them in warm water and wonder in using a softener like conditioner would help. I am new to the animal tissue world and any advise would help. Thanks in advance. Kimberly ------------------------------ The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From mpence <@t> grhs.net Wed Feb 18 12:55:36 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Feb 18 12:56:13 2015 Subject: [Histonet] RE: Bone Saw In-Reply-To: References: <20150218165725.8C01A1EACC5@trendmess-svr.holyredeemer.local> Message-ID: What is clean-up like? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, February 18, 2015 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bone Saw We also use the little table-top band saw from Mar-Med. It has a small footprint, splash guards, and is just awesome for cutting thin beautiful sections. We use toothed forceps to hold the specimen and keep fingers out of the way. Like the man said, a hot knife through soft butter! We have 3 Pathologists and one PA and they all LOVE it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 > I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? We use a small band saw from MAR-MED. cuts through a femoral head like a hot knife through soft butter. You let the blade do the job, don't force the bone. Just very little consistent pressure. Rich Y --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Wed Feb 18 13:04:12 2015 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Wed Feb 18 13:04:19 2015 Subject: [Histonet] RE: Bone Saw In-Reply-To: References: <20150218165725.8C01A1EACC5@trendmess-svr.holyredeemer.local> Message-ID: <003d01d04bad$af3ab4f0$0db01ed0$@caplab.org> In the event you don't have a band saw, I wish I did, we have a vice that has a suction cup bottom that we attach to the top of one of our specimen carts. It holds the bone well enough to get reasonable sections. If I had to do too many more than I do, I'd request a band saw. When I'm done, I just clean up the saw, the cart and I'm done. Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow / CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, February 18, 2015 1:56 PM To: 'Terri Braud'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bone Saw What is clean-up like? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, February 18, 2015 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bone Saw We also use the little table-top band saw from Mar-Med. It has a small footprint, splash guards, and is just awesome for cutting thin beautiful sections. We use toothed forceps to hold the specimen and keep fingers out of the way. Like the man said, a hot knife through soft butter! We have 3 Pathologists and one PA and they all LOVE it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 > I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections? We use a small band saw from MAR-MED. cuts through a femoral head like a hot knife through soft butter. You let the blade do the job, don't force the bone. Just very little consistent pressure. Rich Y ---------------------------------------------------------------------------- ----- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2015.0.5646 / Virus Database: 4284/9137 - Release Date: 02/18/15 From bszpunar <@t> umail.iu.edu Wed Feb 18 13:06:10 2015 From: bszpunar <@t> umail.iu.edu (Bryan Szpunar) Date: Wed Feb 18 13:06:50 2015 Subject: [Histonet] RE: New Lab Setup Message-ID: Hi Jim, While I do not have quite the length of time in the field that you do, I do have been through this several times and have some thoughts. First, to your question about how many samples is acceptable, I would say you should somewhat base this on your acceptability criteria. For example, if acceptability for your validation is 95% of the specimens being "overall acceptable", you should do either 20 or 40 (for your scope, I would pick 20 personally), but NOT somewhere in between those numbers. The reason for this is you gain nothing in the land of percentages by doing, say 25. At 20, any more than one specimen that is "unacceptable" will throw you below 95%. At 40, you can have two outliers and still be within 95%. That is just a hypothetical, but hopefully you get the gist. Essentially, don't give yourself more opportunities to fail the validation if it gains you nothing in the margin. If your application requires a more robust validation, go for 40 (a la prognostic IHC). I would think an IHC/histochemistry validation would be overkill if you are validating your initial samples in parallel anyway. Just be sure to document that the results of the specimens were comparable to each other. I might have a different opinion if you were not using the exact same processors and doing some form of rapid/microwave processing instead. Regards, Bryan > Date: Wed, 18 Feb 2015 17:27:50 +0000 > From: "Vickroy, James" > Subject: [Histonet] New lab setup > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 9B1A1501A800064397369BD8072E6BCA984027@E2K10DB.springfieldclinic.com> > Content-Type: text/plain; charset="us-ascii" > > > I am working on setting up an adequate validation study for tissue > processing at a new lab. I want it to be adequate but also not too labor > intensive or costly. I have been in Histotechnology for over 36 years and > have done this before in the lab I can from. Unfortunately sometimes I > make things more complicated than others. I realize the standards for > testing new protocols and antibodies are defined in the CAP checklist but > routine tissue processing just says to document the procedure and to run > parallel samples as a blind study. > > We are going to run only GI biopsies at first and I had planned on running > parallel samples with the department from my previous employer. I also > thought that down the road we may have more than a rapid biopsy program so > I should do a few larger samples at a regular processing schedule to > validate both schedules (rapid and normal). I also thought that I should > pick out a couple of blocks for representative special stains and IHC > stains tha we would compare even though the new lab will send everything to > the old lab for special stains and IHC stains. > > We have two processors and know I will also have to run a small validation > of each new processor. I figured as I was validating the tissue processing > programs I would also be validating the first tissue processor. > > Both of our two processors will be VIP6(s) and the machines from my former > employer were also VIP6(s) which were of course validated. > > Can anyone share how many samples are adequate for tissue processing > program validation and new processor validation? In other words what have > you done and what was accepted by CAP since the wording of the question > does not state any particulars? Also can you tell me if you think the > special stains and IHC stains comparison is necessary, given that all of > the stains will be done at the old lab and the only difference will be > where the tissues were processed. > > My original design of the study had 25 parallel samples but I am wondering > if that is overkill. > > Thanks > > Jim Vickroy > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy@SpringfieldClinic.com jvickroy@SpringfieldClinic.com> > > > This electronic message contains information from Springfield Clinic, LLP > that may be confidential, privileged, and/or sensitive. This information is > intended for the use of the individual(s) or entity(ies) named above. If > you are not the intended recipient, be aware that disclosure, copying, > distribution, or action taken on the contents of this information is > strictly prohibited. If you have received this electronic message in error, > please notify the sender immediately, by electronic mail, so that > arrangements may be made for the retrieval of this electronic message. > Thank you. > From Fawn.Bomar <@t> HalifaxRegional.com Wed Feb 18 13:48:41 2015 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Wed Feb 18 13:49:32 2015 Subject: [Histonet] Looking for an article Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is "The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue." The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From gmartin <@t> marshallmedical.org Wed Feb 18 13:55:46 2015 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Feb 18 13:56:55 2015 Subject: [Histonet] Release of tissue Message-ID: <6ED9D4252F278841A0593D3D788AF24C29DCEA72@mailsvr.MARSHMED.local> Thanks to all who responded to my requested for information concerning the release of tissue to patients. I seems that most facilities are not releasing tissue, especially if it has been fixed in formalin. Thanks, Gary Martin El Dorado Pathology Med. Grp. Marshall Med. Ctr. Pathology Dept. gmartin@marshallhospital.org This e-mail is only intended for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other then the intended recipient is strictly prohibited. If you receive this communication in error, please contact the sender and destroy all forms of this communication. From anna.coffey <@t> nih.gov Wed Feb 18 13:56:34 2015 From: anna.coffey <@t> nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Wed Feb 18 13:57:10 2015 Subject: [Histonet] Help with cutting mouse brain at 9-10 microns Message-ID: <5C3E10119A1B824FBE92B08279F74A91017811B5@msgb09.nih.gov> Hi Kimberly, Most of what I work with is mouse tissue and I've found the brains to be a bit tricky because they both hydrate and dry out quickly. I normally keep the paraffin blocks on an ice for about 2 hours (after they've been fully faced in), checking periodically to make sure the tissue is not overhydrating. When I section, I can normally only take a few sections before the brain starts to dry out again (you can tell when you start to see scratches and dry white areas on the tissue). Most of the blocks are ready to cut again after a few additional minutes back on the ice. For thicker sections (up to 20um), I take use the wooden stick of a cotton swab and hold it against the base of the paraffin block as I cut the section. The section will curl around the stick and you can roll it out flat on the water bath to smooth it out. Hope this helps! Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 301-846-1730 From azdudley <@t> hotmail.com Wed Feb 18 14:10:48 2015 From: azdudley <@t> hotmail.com (anita) Date: Wed Feb 18 14:11:11 2015 Subject: [Histonet] Bone Marrow Message-ID: How are you billing for bone marrow bxs. we do irons on smear and core plus clot. should we bill for all iron stains? also we do a wrights stain. Anita Dudley Providence Hospital Mobile, Al From azdudley <@t> hotmail.com Wed Feb 18 14:17:49 2015 From: azdudley <@t> hotmail.com (anita) Date: Wed Feb 18 14:18:11 2015 Subject: [Histonet] Job Opening Message-ID: We have a position open for Histology Surpervisor at Providence Hospital in Mobile, Alabama. Online application www.providencehospital.org. Anita Dudley Providence Hospital Mobile, al From mcauliff <@t> rwjms.rutgers.edu Wed Feb 18 14:46:46 2015 From: mcauliff <@t> rwjms.rutgers.edu (Geoff) Date: Wed Feb 18 14:47:03 2015 Subject: [Histonet] Looking for an article In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> Message-ID: <54E4FA36.9060700@umdnj.edu> You can read it here: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768083/ Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: > Hi all, > > > > I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is "The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue." > > > > The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. > > > > Thank you for any help > > > > Fawn > ------------------------------------------------------------- > This electronic message may contain information that is > confidential or legally privileged. It is intended only > for the use of the individual(s) and entity named as recipients > in the message. > > If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from any > computer. Do not deliver, distribute, or copy this message, and > do not disclose its contents or take any action in reliance on > the information it contains. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff@rwjms.rutgers.edu ********************************************** From mcauliff <@t> rwjms.rutgers.edu Wed Feb 18 14:51:22 2015 From: mcauliff <@t> rwjms.rutgers.edu (Geoff) Date: Wed Feb 18 14:51:42 2015 Subject: [Histonet] Looking for an article In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> Message-ID: <54E4FB4A.6090500@umdnj.edu> My error, I sent a link to a similar article. The original is in The Journal of Histotechnology. 2006;29(3):173?176 but my library does not have that journal. Perhaps someone on the list has it. Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: > Hi all, > > > > I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is "The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue." > > > > The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. > > > > Thank you for any help > > > > Fawn > ------------------------------------------------------------- > This electronic message may contain information that is > confidential or legally privileged. It is intended only > for the use of the individual(s) and entity named as recipients > in the message. > > If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from any > computer. Do not deliver, distribute, or copy this message, and > do not disclose its contents or take any action in reliance on > the information it contains. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff@rwjms.rutgers.edu ********************************************** From jstaruk <@t> masshistology.com Wed Feb 18 14:57:13 2015 From: jstaruk <@t> masshistology.com (Mass Histology) Date: Wed Feb 18 14:57:24 2015 Subject: [Histonet] Looking for an article In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> Message-ID: <13e401d04bbd$794fcf00$6bef6d00$@masshistology.com> I'll scan a copy I have and email it to you later today. Jim _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com www.nehorselabs.com www.parascreen.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, February 18, 2015 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for an article Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is "The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue." The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Wed Feb 18 15:02:16 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Feb 18 15:02:55 2015 Subject: [Histonet] Looking for an article In-Reply-To: <54E4FB4A.6090500@umdnj.edu> References: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> <54E4FB4A.6090500@umdnj.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF367F58EC@ex07.net.ucsf.edu> If you are a member of NSH you can get a pdf for free just by contacting the staff at the NSH office. A great reason to join, if you have not already! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Wednesday, February 18, 2015 12:51 PM To: Fawn Bomar; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Looking for an article My error, I sent a link to a similar article. The original is in The Journal of Histotechnology. 2006;29(3):173-176 but my library does not have that journal. Perhaps someone on the list has it. Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: > Hi all, > > > > I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is "The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue." > > > > The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. > > > > Thank you for any help > > > > Fawn > ------------------------------------------------------------- > This electronic message may contain information that is confidential > or legally privileged. It is intended only for the use of the > individual(s) and entity named as recipients in the message. > > If you are not an intended recipient of this message, please notify > the sender immediately and delete the material from any computer. Do > not deliver, distribute, or copy this message, and do not disclose its > contents or take any action in reliance on the information it > contains. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff@rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Feb 18 15:06:09 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Wed Feb 18 15:07:42 2015 Subject: [Histonet] RE: New Lab Setup In-Reply-To: References: Message-ID: Just finished a lab set up and intial CAP inspection. I had VIPs and at least 5 processing programs. I did 20 samples each, documented the tissue type, dimensions, criteria for acceptability, microscopic review/morphology preservation using H & E staining sections, signed off by medical director. I did the same for each separate program using tissue appropriate to the program. Typed up a summary document, retained with validtion slides and blocks. No issues with CAP. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 18 Feb 2015 14:06:10 -0500 > From: bszpunar@umail.iu.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: New Lab Setup > > Hi Jim, > While I do not have quite the length of time in the field that you do, I do > have been through this several times and have some thoughts. > > First, to your question about how many samples is acceptable, I would say > you should somewhat base this on your acceptability criteria. For example, > if acceptability for your validation is 95% of the specimens being "overall > acceptable", you should do either 20 or 40 (for your scope, I would pick 20 > personally), but NOT somewhere in between those numbers. The reason for > this is you gain nothing in the land of percentages by doing, say 25. At > 20, any more than one specimen that is "unacceptable" will throw you below > 95%. At 40, you can have two outliers and still be within 95%. > > That is just a hypothetical, but hopefully you get the gist. Essentially, > don't give yourself more opportunities to fail the validation if it gains > you nothing in the margin. If your application requires a more robust > validation, go for 40 (a la prognostic IHC). > > I would think an IHC/histochemistry validation would be overkill if you are > validating your initial samples in parallel anyway. Just be sure to > document that the results of the specimens were comparable to each other. I > might have a different opinion if you were not using the exact same > processors and doing some form of rapid/microwave processing instead. > > Regards, > Bryan > > > > > > > Date: Wed, 18 Feb 2015 17:27:50 +0000 > > From: "Vickroy, James" > > Subject: [Histonet] New lab setup > > To: "histonet@lists.utsouthwestern.edu" > > > > Message-ID: > > < > > 9B1A1501A800064397369BD8072E6BCA984027@E2K10DB.springfieldclinic.com> > > Content-Type: text/plain; charset="us-ascii" > > > > > > I am working on setting up an adequate validation study for tissue > > processing at a new lab. I want it to be adequate but also not too labor > > intensive or costly. I have been in Histotechnology for over 36 years and > > have done this before in the lab I can from. Unfortunately sometimes I > > make things more complicated than others. I realize the standards for > > testing new protocols and antibodies are defined in the CAP checklist but > > routine tissue processing just says to document the procedure and to run > > parallel samples as a blind study. > > > > We are going to run only GI biopsies at first and I had planned on running > > parallel samples with the department from my previous employer. I also > > thought that down the road we may have more than a rapid biopsy program so > > I should do a few larger samples at a regular processing schedule to > > validate both schedules (rapid and normal). I also thought that I should > > pick out a couple of blocks for representative special stains and IHC > > stains tha we would compare even though the new lab will send everything to > > the old lab for special stains and IHC stains. > > > > We have two processors and know I will also have to run a small validation > > of each new processor. I figured as I was validating the tissue processing > > programs I would also be validating the first tissue processor. > > > > Both of our two processors will be VIP6(s) and the machines from my former > > employer were also VIP6(s) which were of course validated. > > > > Can anyone share how many samples are adequate for tissue processing > > program validation and new processor validation? In other words what have > > you done and what was accepted by CAP since the wording of the question > > does not state any particulars? Also can you tell me if you think the > > special stains and IHC stains comparison is necessary, given that all of > > the stains will be done at the old lab and the only difference will be > > where the tissues were processed. > > > > My original design of the study had 25 parallel samples but I am wondering > > if that is overkill. > > > > Thanks > > > > Jim Vickroy > > > > Jim Vickroy > > Histology Manager > > Springfield Clinic, Main Campus, East Building > > 1025 South 6th Street > > Springfield, Illinois 62703 > > Office: 217-528-7541, Ext. 15121 > > Email: jvickroy@SpringfieldClinic.com > jvickroy@SpringfieldClinic.com> > > > > > > This electronic message contains information from Springfield Clinic, LLP > > that may be confidential, privileged, and/or sensitive. This information is > > intended for the use of the individual(s) or entity(ies) named above. If > > you are not the intended recipient, be aware that disclosure, copying, > > distribution, or action taken on the contents of this information is > > strictly prohibited. If you have received this electronic message in error, > > please notify the sender immediately, by electronic mail, so that > > arrangements may be made for the retrieval of this electronic message. > > Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Feb 18 15:33:34 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Feb 18 15:33:52 2015 Subject: [Histonet] Looking for an article In-Reply-To: <71b0c5202c37d.54e504f4@uwo.ca> References: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> <54E4FB4A.6090500@umdnj.edu> <761E2B5697F795489C8710BCC72141FF367F58EC@ex07.net.ucsf.edu> <7370919728d2d.54e5043a@uwo.ca> <7330aedd2eb7d.54e50477@uwo.ca> <72a0af1d2dd61.54e504b5@uwo.ca> <71b0c5202c37d.54e504f4@uwo.ca> Message-ID: <7330a88c292fb.54e4bede@uwo.ca> If you are a member of NSH you can also get a pdf for free by way of nsh.org. See email copied below. John Kiernan London, Canada = = = Dear All, I am pleased to confirm that the login button for the Journal of Histotechnology Editorial Manager site is now working. You may need to clear your internet browser cache before you can use the button. Instructions on how to do this are available at (http://www.wikihow.com/Clear-Your-Browser%27s-Cache" target="_blank">http://www.wikihow.com/Clear-Your-Browser) My apologies for the inconvenience, With best wishes, Rasheda Begum Editorial Assistant Maney Publishing, 1 Carlton House Terrace, London, SW1Y 5AF, United Kingdom Tel: +44 (0)20 7451 7408 Fax: +44 (0)20 7451 7307 Email: R.Begum@maneypublishing.com _________________________________________ On 18/02/15, "Morken, Timothy" wrote: > If you are a member of NSH you can get a pdf for free just by contacting the staff at the NSH office. A great reason to join, if you have not already! > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff > Sent: Wednesday, February 18, 2015 12:51 PM > To: Fawn Bomar; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Looking for an article > > My error, I sent a link to a similar article. The original is in The Journal of Histotechnology. 2006;29(3):173-176 but my library does not have that journal. Perhaps someone on the list has it. > > Geoff > > On 2/18/2015 2:48 PM, Fawn Bomar wrote: > > Hi all, > > > From brett_connolly <@t> merck.com Wed Feb 18 15:48:19 2015 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Feb 18 15:48:27 2015 Subject: [Histonet] Looking for an article In-Reply-To: <7330a88c292fb.54e4bede@uwo.ca> References: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> <54E4FB4A.6090500@umdnj.edu> <761E2B5697F795489C8710BCC72141FF367F58EC@ex07.net.ucsf.edu> <7370919728d2d.54e5043a@uwo.ca> <7330aedd2eb7d.54e50477@uwo.ca> <72a0af1d2dd61.54e504b5@uwo.ca> <71b0c5202c37d.54e504f4@uwo.ca> <7330a88c292fb.54e4bede@uwo.ca> Message-ID: I sent her the .pdf file I downloaded from Many Pub. Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Wednesday, February 18, 2015 4:34 PM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Looking for an article If you are a member of NSH you can also get a pdf for free by way of nsh.org. See email copied below. John Kiernan London, Canada = = = Dear All, I am pleased to confirm that the login button for the Journal of Histotechnology Editorial Manager site is now working. You may need to clear your internet browser cache before you can use the button. Instructions on how to do this are available at (http://www.wikihow.com/Clear-Your-Browser%27s-Cache" target="_blank">http://www.wikihow.com/Clear-Your-Browser) My apologies for the inconvenience, With best wishes, Rasheda Begum Editorial Assistant Maney Publishing, 1 Carlton House Terrace, London, SW1Y 5AF, United Kingdom Tel: +44 (0)20 7451 7408 Fax: +44 (0)20 7451 7307 Email: R.Begum@maneypublishing.com _________________________________________ On 18/02/15, "Morken, Timothy" wrote: > If you are a member of NSH you can get a pdf for free just by contacting the staff at the NSH office. A great reason to join, if you have not already! > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff > Sent: Wednesday, February 18, 2015 12:51 PM > To: Fawn Bomar; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Looking for an article > > My error, I sent a link to a similar article. The original is in The Journal of Histotechnology. 2006;29(3):173-176 but my library does not have that journal. Perhaps someone on the list has it. > > Geoff > > On 2/18/2015 2:48 PM, Fawn Bomar wrote: > > Hi all, > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From doug.porter <@t> caplab.org Wed Feb 18 15:51:51 2015 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Wed Feb 18 15:52:04 2015 Subject: [Histonet] Looking for an article In-Reply-To: <54E4FA36.9060700@umdnj.edu> References: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> <54E4FA36.9060700@umdnj.edu> Message-ID: <004e01d04bc5$1b2dc7d0$51895770$@caplab.org> I would be curious to know if there was formalin on the processor. They only mentioned processing in a "conventional manner". Just curious! Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow / CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff Sent: Wednesday, February 18, 2015 3:47 PM To: Fawn Bomar; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Looking for an article You can read it here: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768083/ Geoff On 2/18/2015 2:48 PM, Fawn Bomar wrote: > Hi all, > > > > I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is "The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue." > > > > The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. > > > > Thank you for any help > > > > Fawn > ------------------------------------------------------------- > This electronic message may contain information that is confidential > or legally privileged. It is intended only for the use of the > individual(s) and entity named as recipients in the message. > > If you are not an intended recipient of this message, please notify > the sender immediately and delete the material from any computer. Do > not deliver, distribute, or copy this message, and do not disclose its > contents or take any action in reliance on the information it > contains. > > Thank you > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff@rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2015.0.5646 / Virus Database: 4284/9137 - Release Date: 02/18/15 From JWatson <@t> gnf.org Wed Feb 18 16:31:54 2015 From: JWatson <@t> gnf.org (James Watson) Date: Wed Feb 18 16:32:03 2015 Subject: [Histonet] Storing antibodies in a frostless freezer Message-ID: Being old school I was taught that antibodies do not get stored in a frostless freezer. I am now being told that the new frostless freezers do not go above -13?C during the defrost cycle and it is safe to use. Teach this old dog a new trick, is anyone using a frostless freezer for storing antibodies? Jamie James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From barryrittman <@t> gmail.com Wed Feb 18 17:32:36 2015 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Wed Feb 18 17:32:39 2015 Subject: [Histonet] honey versus formalin Message-ID: Sorry but my itchy delete finger got rid of the subscriber who was asking about this. There is a 2014 article by Sabarinath et al in Journal of Histotech. vol 37 pp 21-25. comparing honey and formalin fixation. If you use "honey versus formalin as fixative" and go to the go to www.maneyonline.com refernce you can access the entire article. Barry From rsrichmond <@t> gmail.com Wed Feb 18 18:34:47 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Feb 18 18:34:52 2015 Subject: [Histonet] Re: Looking for an article Message-ID: This article about using honey as a "fixative" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768083/ contends that you can use honey, jaggery sugar (look that up in Wikipedia), or sugar syrup as a "fixative". What you're doing with honey is creating a transport medium - like the chemically more sophisticated "Streck medium" and others promoted to us a few years ago - that preserves the tissue until it can fix in alcohol in the processor. Bob Richmond Samurai Pathologist Maryville TN From rsrichmond <@t> gmail.com Thu Feb 19 03:13:01 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Feb 19 03:14:14 2015 Subject: [Histonet] Re: needle size Message-ID: Jennifer MacDonald asks >>Does anyone have the gauge of needle used for breast core biopsies and for prostate biopsies?<< An 18 gauge needle is standard for transrectal prostate biopsies. Breast stereotactic needle biopsy needles are considerably larger, but you'll need to inquire what your local people are using. Bob Richmond Samurai Pathologist Maryville TN From rsrichmond <@t> gmail.com Thu Feb 19 03:35:55 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Feb 19 03:36:12 2015 Subject: [Histonet] Re: Bone Saw Message-ID: Mike Pence (where?) asks: >>I am trying to see what everyone is using at your grossing station for bone saw to cut femoral heads and toes for osteo. If you are using a Stryker saw how are you holding the specimens to make good thin sections?<< >>Three suggestions were: >>Mopec ZawBones double-bladed saw http://media3.mopec.com/media/pdf/AutopsyAccessories%28Page76%29.pdf >>Dremel tool >>We use a small [table-top] band saw from MAR-MED. cuts through a femoral head like a hot knife through soft butter. You let the blade do the job, don't force the bone. Just very little consistent pressure.<< Well, I've worked in about 80 pathology services, mostly small ones, in the last 50 years, and here's what I've observed. Good Management Practice is of course to deny the pathologist all but the most basic grossing tools. The cowboy way is to do no sawing at all. Jiss chuck the femoral head or tibial plateau into Decal for a few weeks, then cut it with a scalpel. Of course the sections are uninterpretable, IHC is impossible, and you probably can't bill successfully after so long. Pathologists who insist on microscopic examination (to run up the bill) do the decalcification the cowboy way, in my experience. Most common saw is the Satterlee amputation saw (Google it for an illustration). Tried and true - I've seen them in Civil War re-enactments, chrome plating and all. You're fortunate if you have one - they cost about $100 each, so you couldn't get one today. Google it for an illustration. We're having an ice storm here in east Tennessee this week, and yesterday I sawed four femoral heads from little old ladies who'd slipped on the ice. (You hold the femoral head in a wad of paper towels.) If a pathology service doesn't have any kind of a saw, I go to a hardware store and buy whatever hacksaw they have on the shelf, for about $7. They don't last very long. I've once used one of the double-bladed hacksaws, like the MOPEC ZawBones mentioned earlier. Made for medical use, they cost around $500, so no, you can't have one. Some pathologists use the Stryker oscillating saw they use at the autopsy table, but there's no way to immobilize a loose specimen (such as a femoral head) safely, and it's likely to fly up in your face. Also, the Stryker saw overheats the specimen and damages the histology. Band saws and scroll saws are often recommended, but I've never seen one used. They're heavy, have a large footprint on the table, and cost several hundred dollars, so, no, you can't have one. Femoral heads from fractures need microscopic examination, to look for metastatic cancer (pathologic fracture). Osteoarthritis can be gross-only, as can most knee replacement specimens. Most fracture specimens are osteoporotic and easy to cut - I record the difficulty of sawing in the gross description - "the femoral head is slabbed with a hand saw without much difficulty/with considerable difficulty". Bob Richmond Samurai Pathologist Maryville TN From bburnett <@t> CapeCodHealth.org Thu Feb 19 07:46:33 2015 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Thu Feb 19 07:46:39 2015 Subject: [Histonet] tissue fallin off CAP slides Message-ID: Has anyone out there had any issues with tissue falling off of the CAP slides for ER/PgR surveys? I recently found out that they are embedding their cores in a paraffin with a much higher melting point than ours. Could the incomplete removal of paraffin cause the tissue to fall off the slides during target retrieval? They are stating that any detachment of the cores are dependent on the conditions of the retrieval and not on any difference in the tissue paraffin. Any feedback on this would be much appreciated. Thanks, Brandy Burnett, HTL QIHC(ASCP) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From GKeyser <@t> uwhealth.org Thu Feb 19 07:49:26 2015 From: GKeyser <@t> uwhealth.org (Keyser Gerald T) Date: Thu Feb 19 07:50:15 2015 Subject: [Histonet] RE: Looking for an article In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B982F854@EXCH-2K10.hrhs.com> Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE04B130@UWHC-MBX12.uwhis.hosp.wisc.edu> I can't find it without a paywall either. I find the idea of honey as a formalin substitute fascinating. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, February 18, 2015 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for an article Hi all, I need some help in locating an article for class, preferably one I don't have to pay for. The name of the article is "The Effectiveness of Honey as a Substitute for Formalin in the Histological Fixation of Tissue." The only place I found this article so far charges $48 dollars for 24 hour access and I need to read it and write a summary for my class so I need longer than 1 day. Thank you for any help Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Feb 19 08:29:17 2015 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Feb 19 08:29:36 2015 Subject: [Histonet] Instructions for J of Histo free access to archives through Maney Publishing Message-ID: Most of you probably know that NSH members have free access to the JOH archives, but you need to log-in to MY NSH and then register with Maney Publishing to get them. I think you can get access to some JOH articles through PubMed as a non-member, but not all of them. Instructions for members are below . Brett NSH Members First do this... NSH members can review the current issue and archived issues of the Journal of Histotechnology online at the Maney Publishing website by logging into their "MY NSH" account. Click here to access MY NSH & then select Membership Directory/JOH Online. You will need to login using your primary email address on file with NSH and your password to My NSH. If you do not have a password or have questions about your log in please contact the NSH Office, 443-535-4060 or email histo@NSH.org. Then do this... First Time Visitor To obtain your online access to Journal of Histotechnology please go to www.maneyonline.com/loi/his and register by clicking on 'register' link in the top right-hand corner of the screen. Once you have registered, click on your name which will appear in the top right of the screen. This will take you to your 'My account' area of Maney Online. From here, please click on 'Access token' from the left-hand menu, and input the following token: NSHaccess. Click submit. You can then click on 'Access entitlements' in the left-hand menu where you will see a link to the Journal of Histotechnology where will have full access to the journal content. You only have to complete the token process once. On future visits you simply need to click www.maneyonline.com/loi/his , sign-in with your registered email address and password, and you will continue to have access to the journal content. Registered Visitor Click www.maneyonline.com/loi/his , sign-in with your Maney Online registered email address and password, and you will continue to have access to the journal content. Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From relia1 <@t> earthlink.net Thu Feb 19 08:41:44 2015 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Feb 19 08:41:58 2015 Subject: [Histonet] RELIA Histology Careers Bulletin 2-19-2015 Exciting Job Opportunities and An Updated List of State and Regional Histology Meetings. Message-ID: <00bc01d04c52$2eea4a40$8cbedec0$@earthlink.net> RELIA Histology Careers Bulletin 2-19-2015 Exciting New Opportunities and Information on State and Regional Histology Meetings. Hi Histopeeps!! I thought I would drop you a line while we all wait to thaw out. I want to let you know that I have some exciting job opportunities. And I also wanted to pass along the info on some more of the state and regional meetings. The annual symposium, the state society and regional meetings are fun and educational. They give you the opportunity to network with your peers and hang out and have fun with other histotechs. Scroll down for my updated list of state and regional meetings the new listings are highlighted. For further information or information on other areas check the NSH website: www.nsh.org *All of these positions are full time and permanent.* *Our clients offer excellent compensation and benefits.* *Relocation and sign on bonuses are also available with many of our clients.* Here Is A List Of Our Most Exciting Job Opportunities. Leadership Opportunities: Histology Supervisor - Flagstaff, AZ Histology Manager - Chicago, IL Lead Histology Tech - St. Louis, MO Lead Histology Tech/Research - Boston, MA Field Applications Specialist HT/HTL Opportunities: Histotech - Kingsport, TN Histotechnician - Dallas, TX Histology Tech - Hammond, IN ASCP or ELIGIBLE HT/HTL - Williamsburg, VA HT/HTL - Norfolk, VA Field Applications Specialist For more information on any of these opportunities please contact me at relia1@earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. If you are looking for a new opportunity in another area shoot me an email I can be on the lookout for you!! - relia1@earthlink.net **Histology Society of Ohio is April 24-25 at the Beavercreek/Dayton Hilton Garden Inn. Contact Audrey Toothman for more info: aud2002@aol.com **Illinois Society for Histotechnologists May 14-15, 2015 Schaumburg Marriott http://www.illinoishistologysociety.org/meetings.html Or call, Lori 217-202-3820 **Kentucky Society for Histotechnology is having her 39th Annual Symposium on the 27th and the 28th of February, 2015. **The Arkansas Society for Histotechnology - Spring Meeting - March 7th, 2015 in Little Rock, Arkansas. The meeting is being held at the Baptist Schools of Health in Little Rock and is open to anyone in Histology and related or interested fields. For more information: ashnews@comcast.net **The Region I Symposium, taking place July 25-26, 2015 at the Radisson in Manchester NH. Thank you! The meeting is being hosted by the VT/NH Society for Histotechnology. **Tristate Histology Spring Symposium, May 6th-8th at the Concourse Hotel Madison, WI More information on our state society websites; www.mnsfh.wildapricot.org , www.whs.wildapricot.org , Iowa does not have a site. This is a meeting with Minnesota, Wisconsin, and Iowa. **Georgia: HISTOPALOOZA! 2015 at Legacy Lodge at Lake Lanier Islands, April 17~19, 2015 For more information: www.histosearch.com/gsh/ **The Texas Society for Histotechnology will be holding the 2015 S/C in Plano Texas. For more information please visit their website at www.txsh.org or let me know and I can forward the beautiful flyer they sent to me. **Florida: The meeting will be held at the Buena Vista Palace Hotel and Spa in Orlando May 15-17, 2015. For more information: http://www.fshgroup.org/meeting/ Stay Warm!!! Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jvickroy <@t> SpringfieldClinic.com Thu Feb 19 09:39:42 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Thu Feb 19 09:39:50 2015 Subject: [Histonet] Slide and Block Combination Transport or Mailer Message-ID: <9B1A1501A800064397369BD8072E6BCA984D44@E2K10DB.springfieldclinic.com> I have seen somewhere a combination transport or mailer for both a paraffin block and slides. We are going to have our special stains and IHC stains done across town and we use a courier to take the paraffin block to the lab that will do the procedure and then they send back the block and the slide(s). Any ideas which vendor carries something like this? I want to have something in which the block and returned slides will be in the same container. Experience tells me things find a way to get separated and lost. Thanks Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From JRobinson <@t> pathology-associates.com Thu Feb 19 10:27:08 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Thu Feb 19 10:27:29 2015 Subject: [Histonet] RE: Slide and Block Combination Transport or Mailer In-Reply-To: <9B1A1501A800064397369BD8072E6BCA984D44@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA984D44@E2K10DB.springfieldclinic.com> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8C750@PAEXCH1.PathologyAssociates.local> Hi James- I just looked at my exhibit map from Austin and I believe the item you seek (a plastic mailer that holds both block and slide) is sold by Evergreen Scientific. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Thursday, February 19, 2015 7:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Combination Transport or Mailer I have seen somewhere a combination transport or mailer for both a paraffin block and slides. We are going to have our special stains and IHC stains done across town and we use a courier to take the paraffin block to the lab that will do the procedure and then they send back the block and the slide(s). Any ideas which vendor carries something like this? I want to have something in which the block and returned slides will be in the same container. Experience tells me things find a way to get separated and lost. Thanks Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From ecameron1 <@t> midcoasthealth.com Thu Feb 19 12:32:07 2015 From: ecameron1 <@t> midcoasthealth.com (Cameron, Elizabeth) Date: Thu Feb 19 12:32:29 2015 Subject: [Histonet] Primera Signature and Microbiopsy Cassettes Message-ID: I am looking for recommendations for microbiopsy cassettes to use on the Primera Signature printer. I have two different types (Leica LP C and Simport Micromesh), and neither of them print as clearly as the standard Tissue Tek cassettes. Thanks in advance. Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 From ecameron1 <@t> midcoasthealth.com Thu Feb 19 12:40:58 2015 From: ecameron1 <@t> midcoasthealth.com (Cameron, Elizabeth) Date: Thu Feb 19 12:41:04 2015 Subject: [Histonet] RE: Primera Signature and Microbiopsy Cassettes - addendum Message-ID: Just wanted to add that I have used Tissue Tek Mesh biopsy cassettes in the past and experienced issues with processing. I would also be interested in hearing from people who use the Mesh either successfully or unsuccessfully to determine whether that is an option. Thanks again. From: Cameron, Elizabeth Sent: Thursday, February 19, 2015 1:32 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Primera Signature and Microbiopsy Cassettes I am looking for recommendations for microbiopsy cassettes to use on the Primera Signature printer. I have two different types (Leica LP C and Simport Micromesh), and neither of them print as clearly as the standard Tissue Tek cassettes. Thanks in advance. Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 From CThornton <@t> dahlchase.com Thu Feb 19 12:52:56 2015 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Thu Feb 19 12:53:32 2015 Subject: [Histonet] c-myc Message-ID: Is anyone running this antibody successfully on the Ventana Ultra or Benchmark platform? What protocol are you using? I am using the Epitomics clone. How did you validate, did you have MYC FISH results to correlate with? Is there a lab that is successfully running this antibody that I could compare my validation results with? This antibody, quite honestly, makes me very nervous. Any advice or suggestions are greatly appreciated. Thank you! Clare Clare J. Thornton, HTL(ASCP),QIHC Lead Immunohistochemistry Tech Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From chapmanc <@t> health.missouri.edu Thu Feb 19 14:19:11 2015 From: chapmanc <@t> health.missouri.edu (Chapman, Cherie J.) Date: Thu Feb 19 14:19:19 2015 Subject: [Histonet] (no subject) Message-ID: Does anyone know for CAP accreditation if you can still use a regular microwave in a lab? Thanks, Cherie From BZIMMERM <@t> gru.edu Thu Feb 19 15:23:14 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Feb 19 15:23:33 2015 Subject: [Histonet] HISTOPALOOZA GSH 2015 Message-ID: Well, here I am in Augusta, Georgia today freezing. Feel like I'm somewhere around the Arctic Circle today, without my dog sled. I'm wishing it was time for Histopalooza! I think it's going to be a great weekend of relaxation and fun learning. Today's featured speaker is Skip Brown. He is the historian for the NSH whose first NSH meeting was 1980 in Atlanta, Georgia. Wanda first met Skip in 1980 at that particular meeting. I think I was about 3 years old at that time. Cricket cricket... Skip will be presenting a management workshop titled "Leadership- The Pinnacle of Management and Leadership". I was in his class in Austin when he presented it and I found it to be an excellent class. I didn't even have to throw back a 5 hour energy drink to stay awake! Skip will also be doing a workshop titled "Optimization of Microtomy". It's a proactive approach to microtomy and I don't think you will snooze in there. When you arrive, reach out to Skip and his lovely bride. They are an interesting couple and a lot of fun. Billie Zimmerman GSH Secretary From Caroline.Pratt <@t> uphs.upenn.edu Thu Feb 19 15:59:26 2015 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Thu Feb 19 15:59:49 2015 Subject: [Histonet] Question about POC testing Message-ID: <14D6A469D20B4F4AACC47C3E973C3FA8CB957C@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Does anyone know if a Tzanck Smear falls under Virology or Cytology for POC testing in a Dermatology clinic. I have heard conflicting theories. Thanks! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 From akelley <@t> path.wustl.edu Thu Feb 19 15:59:56 2015 From: akelley <@t> path.wustl.edu (Kelley, Amanda) Date: Thu Feb 19 16:01:05 2015 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Hello my friend, I hope you are well. Sorry Cherie, but the CAP checklist requires that you use a Lab grade microwave. I would recommend that you order the BP-110 laboratory grade microwave, it must be vented. Since you're in Missouri, I would recommend contacting Lab Storage systems in St. Peter's Mo. 1-800-345-4167 Amanda Kelley HTL Dermatopathology Center at Washington University Cortex One Suite 212 Phone: 314-362-5759 Fax: 314-362-5701 akelley@path.wustl.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie J. Sent: Thursday, February 19, 2015 2:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Does anyone know for CAP accreditation if you can still use a regular microwave in a lab? Thanks, Cherie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From suetp918 <@t> comcast.net Thu Feb 19 16:44:48 2015 From: suetp918 <@t> comcast.net (suetp918) Date: Thu Feb 19 16:45:27 2015 Subject: [Histonet] Question about POC testing Message-ID: Cyto Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: "Pratt, Caroline" Date:02/19/2015 4:59 PM (GMT-05:00) To: 'histonet' Subject: [Histonet] Question about POC testing Does anyone know if a Tzanck Smear falls under Virology or Cytology for POC testing in a Dermatology clinic. I have heard conflicting theories. Thanks! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcman2010 <@t> hotmail.com Fri Feb 20 03:55:11 2015 From: ihcman2010 <@t> hotmail.com (Glen) Date: Fri Feb 20 03:55:26 2015 Subject: [Histonet] from: Glen Message-ID: <4E4603EF-C41F-49E2-B965-8371F049155F@macanette.com> Hiya histonet http://oguzhanaslan.com.tr/leave.php?lead=6cx605hpcxwqeayus ihcman2010@hotmail.com Sent from my iPhone From Wanda.Smith <@t> HCAhealthcare.com Fri Feb 20 08:23:12 2015 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Feb 20 08:23:29 2015 Subject: [Histonet] RE: Question about POC testing In-Reply-To: <14D6A469D20B4F4AACC47C3E973C3FA8CB957C@UPHMASPHI030.UPHS.PENNHEALTH.PRV> References: <14D6A469D20B4F4AACC47C3E973C3FA8CB957C@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Message-ID: <4A1B8A80249114499C2812ADCDA7250902F48B@XRDCWPMSGHCMD1A.hca.corpad.net> Cytology here. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Thursday, February 19, 2015 4:59 PM To: 'histonet' Subject: [EXTERNAL] [Histonet] Question about POC testing Does anyone know if a Tzanck Smear falls under Virology or Cytology for POC testing in a Dermatology clinic. I have heard conflicting theories. Thanks! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kim-lake <@t> uiowa.edu Fri Feb 20 08:36:13 2015 From: kim-lake <@t> uiowa.edu (Lake, Kim S) Date: Fri Feb 20 08:36:19 2015 Subject: [Histonet] Which kind of blade carrier? Message-ID: I received approval to order a new microtome (yay!), and we have chosen a Thermo Scientific HM 300 Series Rotary Microtome to replace our Leitz 1512. My question is, which kind of blade carrier should I choose, the E or ER? We use high profile disposable blades. I can't tell from the tiny pictures what the difference is, and a search of histonet didn't help. Anyone have any advice? Thanks! Kim Lake, MLS (ASCP)CM Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry From Toni.Rathborne <@t> rwjuh.edu Fri Feb 20 08:43:29 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Fri Feb 20 08:44:05 2015 Subject: [Histonet] RE: Which kind of blade carrier? In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F483D0@vap1014.win.rwjuh.edu> Did you have this microtome in to demo? If so, what was used at that time? If not, is there an opportunity to do so before getting a PO? I can tell you that every microtome, tissue processor, stainer, coverslipper, and IHC instrument we have ever purchased has been brought in as a demo first. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S Sent: Friday, February 20, 2015 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Which kind of blade carrier? I received approval to order a new microtome (yay!), and we have chosen a Thermo Scientific HM 300 Series Rotary Microtome to replace our Leitz 1512. My question is, which kind of blade carrier should I choose, the E or ER? We use high profile disposable blades. I can't tell from the tiny pictures what the difference is, and a search of histonet didn't help. Anyone have any advice? Thanks! Kim Lake, MLS (ASCP)CM Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Fri Feb 20 09:14:32 2015 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri Feb 20 09:15:42 2015 Subject: [Histonet] RE: Question about POC testing In-Reply-To: <4A1B8A80249114499C2812ADCDA7250902F48B@XRDCWPMSGHCMD1A.hca.corpad.net> References: <14D6A469D20B4F4AACC47C3E973C3FA8CB957C@UPHMASPHI030.UPHS.PENNHEALTH.PRV> <4A1B8A80249114499C2812ADCDA7250902F48B@XRDCWPMSGHCMD1A.hca.corpad.net> Message-ID: <450B7A81EDA0C54E97C53D60F00776C32337384212@isexstore03> Cytology. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Friday, February 20, 2015 9:23 AM To: Caroline.Pratt@uphs.upenn.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Question about POC testing Cytology here. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline Sent: Thursday, February 19, 2015 4:59 PM To: 'histonet' Subject: [EXTERNAL] [Histonet] Question about POC testing Does anyone know if a Tzanck Smear falls under Virology or Cytology for POC testing in a Dermatology clinic. I have heard conflicting theories. Thanks! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From king.laurie <@t> marshfieldclinic.org Fri Feb 20 12:49:25 2015 From: king.laurie <@t> marshfieldclinic.org (King, Laurie J) Date: Fri Feb 20 12:49:31 2015 Subject: [Histonet] Linear Stainers Message-ID: <7578207839F50248A7A6CD33517295EA4F3DD58D@MCL-EXMB03.mfldclin.org> Hello all- I have worked with the GLX Linear Stainer in the past, while working in Mohs. It worked really well. My question is for those of you out there that have used the smaller version that fits on top of the cryostat. Are there enough stations to get a quality stain? If you are a user, can you share pros and cons please? Laurie King ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From BZIMMERM <@t> gru.edu Fri Feb 20 15:04:46 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Feb 20 15:05:11 2015 Subject: [Histonet] HISTOPALOOZA GEORGIA SOCIETY FOR HISTOTECHNOLOGY April 17-19 Message-ID: TGIF Hopefully, by now everyone has renewed their membership to the GSH. In an effort to keep the cost of CEU's affordable, we offer free membership because of the generosity of our vendor sponsors. Our big shindig of the year is HISTOPALOOZA! and our BOD continues to work tirelessly to bring histotechs an educational experience with some fun thrown in to make it enjoyable. We don't want you to choose to have a root canal instead of getting your continuing education credits. If you don't know what the southern expression "shindig" means please consult Webster's and NOT the urban dictionary. Stay tuned next week when I start doing blurbs on the speakers. Billie Zimmerman GSH secretary From mucram11 <@t> comcast.net Fri Feb 20 15:23:38 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri Feb 20 15:23:53 2015 Subject: [Histonet] MARCH 7TH ARKANSAS SOCIETY OF HISTOTECHNOLOGY MEETING IN LITTLE ROCK In-Reply-To: <378471038.4091981.1424467368068.JavaMail.zimbra@comcast.net> Message-ID: <1234591711.4092180.1424467418893.JavaMail.zimbra@comcast.net> Good Morning All, THE ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH, 2015 IN LITTLE ROCK, ARKANSAS PLEASE JOIN US!! The meeting is being held at the Baptist Schools of Health in Little Rock and is open to anyone in Histology and related or interested fields. The following list is for the speakers, who will all be presenting 90 minute seminars for the meeting. We will have two rooms of speakers so you have a choice in classes and topics for the day. The cost for all day at the classes listed is $60.00 and this includes HT Readiness. 7:30AM Registration 8AM to 4:30PM Shane Jones will be presenting HT Readiness for anyone getting ready to take the HT this spring or summer. 8AM to 9:30AM Seminars Dr Ericka Olgaard from UAMS Diagnostic Challenges of Breast Cancer Dr Daisy Alapat UAMS Methods and Basics of Protein Detection in Hematopathology 9:30AM to 10:00AM BREAK 10:00AM to 11:30AM Seminars Dr Shree Sharma Nephropath Integrating Mobile Technology With Anatomic Pathology Debbie Siena Technical Specialist Statlab Controlling Your Special Stains 11:30AM to 1:00PM Lunch 1:00PM to 2:30PM Seminars Dr Sara Shalin UAMS How and Why Skin Is Different Jennifer Feldman Ventana Antibody Production 2:30PM to 3:00PM Break 3:00PM to 4:30PM Seminars Dr Jennifer Forsythe ME Little Rock Autopsy in America Pamela Marcum HT UAMS Reagent Alcohol - Can't Drink It - What Is It? If you have any questions please let me know and I will attempt to answer ASAP. We are unable to take credit cards so please be aware the checks or cash will required. Thank You, Pam Marcum From brandihisto <@t> hotmail.com Sat Feb 21 10:06:31 2015 From: brandihisto <@t> hotmail.com (Brandi) Date: Sat Feb 21 10:07:33 2015 Subject: [Histonet] Document control Message-ID: My lab manager & I are unhappy with our current document control system. We currently have the lab director sign on the last page of each procedure. This can get fairly long and then we have to keep each page to prove that it was signed. We also list all documents in a spreadsheet and document last revised/approved date. We went on a CAP inspection last year and was impressed with their process. They keep a spreadsheet at the front of all procedure manuals, listing each procedure and the lab director signs by each procedure. Our question with changing to that system is how to use the spreadsheet when you have more than one revision in a year. I'm interested in what other labs are doing, if anyone would be so kind as to share their system I would greatly appreciate it. We do not have a secure signature system, so it can't be electronic. Thank you, Brandi Farris From rob <@t> foliobio.com Sat Feb 21 10:59:12 2015 From: rob <@t> foliobio.com (Rob Day) Date: Sat Feb 21 10:59:39 2015 Subject: [Histonet] Document control In-Reply-To: References: Message-ID: <6D73E974-D9D0-4550-8677-D7A8FD24EC06@foliobio.com> Have you looked at a scientific document management system / ELN like CERF that includes the capacity to manage controlled documents and protocols? > On Feb 21, 2015, at 11:06 AM, Brandi wrote: > > My lab manager & I are unhappy with our current document control system. We currently have the lab director sign on the last page of each procedure. This can get fairly long and then we have to keep each page to prove that it was signed. We also list all documents in a spreadsheet and document last revised/approved date. We went on a CAP inspection last year and was impressed with their process. They keep a spreadsheet at the front of all procedure manuals, listing each procedure and the lab director signs by each procedure. Our question with changing to that system is how to use the spreadsheet when you have more than one revision in a year. I'm interested in what other labs are doing, if anyone would be so kind as to share their system I would greatly appreciate it. We do not have a secure signature system, so it can't be electronic. > > Thank you, > Brandi Farris > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: 614-407-4547 Fax: 877-633-6442 skype: invasifspecies http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. Visit my blogs here. From esulkosky <@t> gmail.com Sun Feb 22 15:45:52 2015 From: esulkosky <@t> gmail.com (Eric Sulkosky) Date: Sun Feb 22 15:48:32 2015 Subject: [Histonet] PCR Testing Message-ID: Hello Histonetter?s, I hope this post finds you all well. I am interested in finding out how many histology labs are currently performing PCR testing? I am particularly interested in having a conversation or email exchange with individuals that are currently performing T cell and B cell gene re-arrangements and infectious disease. I would appreciate any information or feedback. Feel free to contact me off line at esulkosky@gmail.com I look forward to hearing from you soon. Best regards, Eric Sulkosky From esulkosky <@t> gmail.com Sun Feb 22 15:48:09 2015 From: esulkosky <@t> gmail.com (Eric Sulkosky) Date: Sun Feb 22 15:48:51 2015 Subject: [Histonet] PCR Testing Message-ID: Hello Histonetter?s, I hope this post finds you all well. I am interested in finding out how many histology labs are currently performing PCR testing? I am particularly interested in having a conversation or email exchange with individuals that are currently performing T cell and B cell gene re-arrangements and infectious disease. I would appreciate any information or feedback. Feel free to contact me off line at esulkosky@gmail.com I look forward to hearing from you soon. Best regards, Eric Sulkosky From esulkosky <@t> gmail.com Sun Feb 22 15:48:53 2015 From: esulkosky <@t> gmail.com (Eric Sulkosky) Date: Sun Feb 22 15:49:01 2015 Subject: [Histonet] PCR Testing Message-ID: <0F5BFAF3-8249-400D-BED7-14BFE7BC981B@gmail.com> Hello Histonetter?s, I hope this post finds you all well. I am interested in finding out how many histology labs are currently performing PCR testing? I am particularly interested in having a conversation or email exchange with individuals that are currently performing T cell and B cell gene re-arrangements and infectious disease. I would appreciate any information or feedback. Feel free to contact me off line at esulkosky@gmail.com I look forward to hearing from you soon. Best regards, Eric Sulkosky From chesarato <@t> hotmail.com Sun Feb 22 19:46:19 2015 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Sun Feb 22 19:46:37 2015 Subject: [Histonet] No Frost Freezers ! Message-ID: I haven?t seen any answer to the question below, and I am curious about it. C?sar Romero Buenos Aires Argentina Message: 19 Date: Wed, 18 Feb 2015 22:31:54 +0000 From: James Watson Subject: [Histonet] Storing antibodies in a frostless freezer To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Being old school I was taught that antibodies do not get stored in a frostless freezer. I am now being told that the new frostless freezers do not go above -13?C during the defrost cycle and it is safe to use. Teach this old dog a new trick, is anyone using a frostless freezer for storing antibodies? Jamie James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org From rachel <@t> gbi-inc.com Sun Feb 22 21:55:53 2015 From: rachel <@t> gbi-inc.com (Rachel M Gonzalez) Date: Sun Feb 22 21:56:01 2015 Subject: [Histonet] No Frost Freezers ! In-Reply-To: References: Message-ID: Hi I thought that the frost-less freezer had two problems. 1) one temperature change 2) Drying out products unless secondary seal was applied such as paraffin. Rachel On Sun, Feb 22, 2015 at 5:46 PM, Cesar Francisco Romero < chesarato@hotmail.com> wrote: > > > I haven?t seen any answer to the question below, and I > am curious about it. > > C?sar Romero > > Buenos Aires > > Argentina > > Message: 19 > > Date: Wed, 18 Feb 2015 22:31:54 +0000 > > From: James Watson > > Subject: [Histonet] Storing antibodies in a frostless freezer > > To: "'histonet@lists.utsouthwestern.edu'" > > > > Message-ID: > > > > Content-Type: text/plain; charset="iso-8859-1" > > > > Being old school I was taught that antibodies do not get stored in a > frostless > freezer. I am now being told that the > new frostless freezers do not go above -13?C during the defrost cycle and > it is > safe to use. Teach this old dog a new > trick, is anyone using a frostless freezer for storing antibodies? > > > > Jamie > > > > James Watson HT ASCP > > GNF Genomics Institute of the Novartis > Research Foundation > > Scientific Technical Leader II, Histology > > Tel 858-332-4647 > > Fax > 858-812-1915 > > jwatson@gnf.org > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From robert.jacox <@t> thermofisher.com Mon Feb 23 06:03:38 2015 From: robert.jacox <@t> thermofisher.com (Jacox, Robert A.) Date: Mon Feb 23 06:03:57 2015 Subject: [Histonet] Which kind of blade carrier? In-Reply-To: References: Message-ID: <7C96E7EF-1533-4F6D-8D01-0F0424172959@thermofisher.com> Kim, I like the E blade holder. I think it is a more robust platform and has less moving parts. The ER is good if you are doing mega cassettes and will accommodate larger blocks. Let me know if you need more information Sent from my iPhone > On Feb 20, 2015, at 9:37 AM, Lake, Kim S wrote: > > I received approval to order a new microtome (yay!), and we have chosen a Thermo Scientific HM 300 Series Rotary Microtome to replace our Leitz 1512. My question is, which kind of blade carrier should I choose, the E or ER? We use high profile disposable blades. I can't tell from the tiny pictures what the difference is, and a search of histonet didn't help. Anyone have any advice? > > Thanks! > > Kim Lake, MLS (ASCP)CM > Laboratory Manager > Oral Pathology Laboratory > University of Iowa College of Dentistry > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Mon Feb 23 11:51:14 2015 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Feb 23 11:51:34 2015 Subject: [Histonet] RE: Histonet Digest, Vol 135, Issue 21 In-Reply-To: References: Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D4EBDC0@D1PWPEXMBX05.mdanderson.edu> She can also google Bard, which a manufacturer of the instruments, and call them. I did and the sales rep that responded helped me to understand what I was asking and gave me the answer. She also sent me a few sample instruments for my student lab. Here is what my rep said: > Breast is usually 14 or 16 ga and 10 cm in length. > > Liver / lung / kidney / prostate is usually 18 ga or 20 ga but length can vary. Your length options are 10, 16 and 20. Hope this helps. Toysha Mayer Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 3 Date: Thu, 19 Feb 2015 04:13:01 -0500 From: Bob Richmond Subject: [Histonet] Re: needle size To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Jennifer MacDonald asks >>Does anyone have the gauge of needle used for breast core biopsies and for prostate biopsies?<< An 18 gauge needle is standard for transrectal prostate biopsies. Breast stereotactic needle biopsy needles are considerably larger, but you'll need to inquire what your local people are using. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ From ynwang <@t> u.washington.edu Mon Feb 23 11:53:51 2015 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Mon Feb 23 11:54:24 2015 Subject: [Histonet] gelatin Message-ID: Hello, Does anyone know of a stain specific for gelatin? I would like to distinguish between firbous collagen and gelatin in treated tissue. thank you Yak-Nam University of Washington Seattle, WA From tejohnson <@t> genoptix.com Mon Feb 23 12:28:54 2015 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Mon Feb 23 12:29:03 2015 Subject: [Histonet] Re: Storing antibodies in a frostless freezer Message-ID: I agree with Rachel. I would not be quite as worried about the temperature change if indeed they maintain no higher than -13 degrees C and would not be freeze/thawing, but what does your ice cube tray look like after about a month or more in a frost-free freezer? Best wishes, Teri Johnson, HT(ASCP)QIHC Genoptix, Inc. A Novartis Company Carlsbad, CA ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From jkiernan <@t> uwo.ca Mon Feb 23 23:47:50 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Feb 23 23:48:00 2015 Subject: [Histonet] gelatin In-Reply-To: <7390f10a164c2.54ec1071@uwo.ca> References: <73c0dcdb16c2f.54ec0d4d@uwo.ca> <7340bb0812783.54ec0d89@uwo.ca> <739091ff10f97.54ec0dc7@uwo.ca> <73908f9416392.54ec0e05@uwo.ca> <737080d3111c3.54ec0e43@uwo.ca> <7310982212109.54ec0e81@uwo.ca> <7380b38a112e6.54ec0ebf@uwo.ca> <73909cbc16334.54ec0efd@uwo.ca> <73c098ae13f0a.54ec0f3b@uwo.ca> <73c0dd6c107d0.54ec0f79@uwo.ca> <7290c2ec17867.54ec0fb7@uwo.ca> <73809e8f1288d.54ec0ff5@uwo.ca> <7390dcf9173d5.54ec1033@uwo.ca> <7390f10a164c2.54ec1071@uwo.ca> Message-ID: <7390897614c8f.54ebca36@uwo.ca> You need to explain "treated tissue". Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope. If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. John Kiernan London, Canada = = = On 23/02/15, Yak-Nam Wang wrote: > Hello, > > Does anyone know of a stain specific for gelatin? I would like to > distinguish between firbous collagen and gelatin in treated tissue. > > thank you > > Yak-Nam > > University of Washington > Seattle, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Ryan.Roy <@t> va.gov Tue Feb 24 09:06:11 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Tue Feb 24 09:08:20 2015 Subject: [EXTERNAL] Re: [Histonet] gelatin In-Reply-To: <7390897614c8f.54ebca36@uwo.ca> References: <73c0dcdb16c2f.54ec0d4d@uwo.ca> <7340bb0812783.54ec0d89@uwo.ca> <739091ff10f97.54ec0dc7@uwo.ca> <73908f9416392.54ec0e05@uwo.ca> <737080d3111c3.54ec0e43@uwo.ca> <7310982212109.54ec0e81@uwo.ca> <7380b38a112e6.54ec0ebf@uwo.ca> <73909cbc16334.54ec0efd@uwo.ca> <73c098ae13f0a.54ec0f3b@uwo.ca> <73c0dd6c107d0.54ec0f79@uwo.ca> <7290c2ec17867.54ec0fb7@uwo.ca> <73809e8f1288d.54ec0ff5@uwo.ca> <7390dcf9173d5.54ec1033@uwo.ca> <7390f10a164c2.54ec1071@uwo.ca> <7390897614c8f.54ebca36@uwo.ca> Message-ID: <15F883394EAB744E99E1C7E1B98730490178A821D213@R04BYNMSGB1.r04.med.va.gov> That's interesting, I didn't realize gelatin is the water soluable protein of collagen. No experience with staining gelatin, but have you considered MassonTrichrome. It is used to differentiate collagen from smooth muscle... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Tuesday, February 24, 2015 12:48 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] gelatin You need to explain "treated tissue". Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope. If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. John Kiernan London, Canada = = = On 23/02/15, Yak-Nam Wang wrote: > Hello, > > Does anyone know of a stain specific for gelatin? I would like to > distinguish between firbous collagen and gelatin in treated tissue. > > thank you > > Yak-Nam > > University of Washington > Seattle, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Tue Feb 24 09:21:12 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Feb 24 09:21:18 2015 Subject: [Histonet] How dark is dark enough? Message-ID: Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula :-) From ynwang <@t> u.washington.edu Tue Feb 24 09:30:19 2015 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Tue Feb 24 09:30:58 2015 Subject: [Histonet] gelatin In-Reply-To: <7390897614c8f.54ebca36@uwo.ca> References: <73c0dcdb16c2f.54ec0d4d@uwo.ca> <7340bb0812783.54ec0d89@uwo.ca> <739091ff10f97.54ec0dc7@uwo.ca> <73908f9416392.54ec0e05@uwo.ca> <737080d3111c3.54ec0e43@uwo.ca> <7310982212109.54ec0e81@uwo.ca> <7380b38a112e6.54ec0ebf@uwo.ca> <73909cbc16334.54ec0efd@uwo.ca> <73c098ae13f0a.54ec0f3b@uwo.ca> <73c0dd6c107d0.54ec0f79@uwo.ca> <7290c2ec17867.54ec0fb7@uwo.ca> <73809e8f1288d.54ec0ff5@uwo.ca> <7390dcf9173d5.54ec1033@uwo.ca> <7390f10a164c2.54ec1071@uwo.ca> <7390897614c8f.54ebca36@uwo.ca> Message-ID: Thank you for your e-mail. Apologies for not explaining "treated tissue". We treat the tissue with high intensity focused ultrasound. It can raise the temperature of tissue to boiling in a localized area (millimeter areas). I could use a biochemical assay for collagen and gelatin if we treat a large area, but with single lesions I was hoping I could visualize this. In some treated areas we are almost resulting in liquefaction of the tissue. I am interested to see if we are turning the collagen to gelatin in these areas and what part of the lesion this is happening. Thank you for your thoughts Yak-Nam On Mon, Feb 23, 2015 at 9:47 PM, John Kiernan wrote: > You need to explain "treated tissue". > > Gelatin is collagen that has been boiled until the protein has lost all > its fibrous nature and changed into a water-soluble protein. Gelatin is > made permanently insoluble by adequate formaldehyde fixation. It is stained > by anionic dyes (such as eosin in the H&E method), but it does not show as > fibres when you look at the section or smear through a microscope. > > If this doesn't answer your question, please explain your problem and > involve your boss in future email exchanges. > > *John Kiernan* > London, Canada > = = = > > On 23/02/15, *Yak-Nam Wang * wrote: > > Hello, > > Does anyone know of a stain specific for gelatin? I would like to > distinguish between firbous collagen and gelatin in treated tissue. > > thank you > > Yak-Nam > > University of Washington > Seattle, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Tue Feb 24 09:37:09 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 24 09:37:34 2015 Subject: [Histonet] How dark is dark enough? In-Reply-To: References: Message-ID: <1238200618.5179661.1424792229766.JavaMail.yahoo@mail.yahoo.com> As long as your microscope has a good Hg source light, it is not really necessary to darken the room so much.?I used to have a very small room were the microscope was located but later on we moved the microscope to another area were we did not dim the light at all and the vision was OK.Everything depends on the circumstances and how comfortable?you are with the image.There are no rules?on this subject.Ren? J.? On Tuesday, February 24, 2015 10:21 AM, Paula Sicurello wrote: Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula? :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison.Scott <@t> harrishealth.org Tue Feb 24 09:45:12 2015 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Tue Feb 24 09:45:44 2015 Subject: [Histonet] Creutzfeldt-Jakob Disease Message-ID: Hello to all in histoland. Does anyone have a procedure for handling creutzfeldt-jakob disease. Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From DSiena <@t> statlab.com Tue Feb 24 09:57:54 2015 From: DSiena <@t> statlab.com (Debra Siena) Date: Tue Feb 24 09:58:16 2015 Subject: [Histonet] RE: Creutzfeldt-Jakob Disease In-Reply-To: References: Message-ID: Hi Allison, I would suggest going to the CDC website and pulling from there, they should have the latest recommendations. thanks Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Tuesday, February 24, 2015 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Creutzfeldt-Jakob Disease Hello to all in histoland. Does anyone have a procedure for handling creutzfeldt-jakob disease. Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Tue Feb 24 10:06:37 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Feb 24 10:09:28 2015 Subject: [Histonet] How dark is dark enough? In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367F6B5C@ex07.net.ucsf.edu> If you are using an automated stainer the plexiglas cover that they all have will block UV light. For instance we run all our IF in a Dako stainer with the slightly tinted Plexiglas and have not had any problems. If staining manually in a tray, covering with something to block light is ok. On the other hand, I can't really say I've had a problem even with no light blocking. The incubations are usually so short it may not make a difference. Consider that the focused UV light in the fluorescence scope is thousands of times stronger than any overhead tube and it still takes a while for the signal to dim. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, February 24, 2015 7:21 AM To: HistoNet Subject: [Histonet] How dark is dark enough? Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ryan.Roy <@t> va.gov Tue Feb 24 10:41:41 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Tue Feb 24 10:42:46 2015 Subject: [Histonet] CBG recycler Message-ID: <15F883394EAB744E99E1C7E1B98730490178A821D214@R04BYNMSGB1.r04.med.va.gov> Hello histonet, Has anyone out there dealt with a busted Heat Resistor in a CBG reagent recycler. Any thoughts or insights appreciated. Thanks in advance, Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA From galinadeyneko <@t> yahoo.com Tue Feb 24 13:21:24 2015 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Tue Feb 24 13:25:39 2015 Subject: [Histonet] Re: gelatin Message-ID: <1444949033.9726433.1424805684821.JavaMail.yahoo@mail.yahoo.com> ?Thank you Dr. Kiernan, Nice and scientific explanation how to prepare gelatin.?I beleive that a lot of house wives and cooks know how to prepare meet or fish jelly (long bouling of the bones with cartilage and tendons). very popular in russian or easter europian cuisine, called "cholodez" which neans "cold".best regards?Galina DeynekoNovartis, Cambridge, MA?617-871-7613 w From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 31, 1969 7:00 PM Subject: Histonet Digest, Vol 135, Issue 25 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Re: Storing antibodies in a frostless freezer (Teri Johnson) ? 2. Re: gelatin (John Kiernan) ? 3. RE: [EXTERNAL] Re: [Histonet] gelatin (Roy, Ryan) ? 4. How dark is dark enough? (Paula Sicurello) ? 5. Re: gelatin (Yak-Nam Wang) ? 6. Re: How dark is dark enough? (Rene J Buesa) ? 7. Creutzfeldt-Jakob? Disease (Scott, Allison D) ? 8. RE: Creutzfeldt-Jakob? Disease (Debra Siena) ? 9. RE: How dark is dark enough? (Morken, Timothy) ? 10. CBG recycler (Roy, Ryan) ---------------------------------------------------------------------- Message: 1 Date: Mon, 23 Feb 2015 18:28:54 +0000 From: Teri Johnson Subject: [Histonet] Re: Storing antibodies in a frostless freezer To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset=WINDOWS-1252 I agree with Rachel. I would not be quite as worried about the temperature change if indeed they maintain no higher than -13 degrees C and would not be freeze/thawing, but what does your ice cube tray look like after about a month or more in a frost-free freezer? Best wishes, Teri Johnson, HT(ASCP)QIHC Genoptix, Inc. A Novartis Company Carlsbad, CA ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Tue, 24 Feb 2015 00:47:50 -0500 From: John Kiernan Subject: Re: [Histonet] gelatin To: Yak-Nam Wang , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: <7390897614c8f.54ebca36@uwo.ca> Content-Type: text/plain; CHARSET=US-ASCII You need to explain "treated tissue". Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope. If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. John Kiernan London, Canada = = = On 23/02/15, Yak-Nam Wang? wrote: > Hello, > > Does anyone know of a stain specific for gelatin? I would like to > distinguish between firbous collagen and gelatin in treated tissue. > > thank you > > Yak-Nam > > University of Washington > Seattle, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 3 Date: Tue, 24 Feb 2015 10:06:11 -0500 From: "Roy, Ryan" Subject: RE: [EXTERNAL] Re: [Histonet] gelatin To: 'John Kiernan' , Yak-Nam Wang ??? , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <15F883394EAB744E99E1C7E1B98730490178A821D213@R04BYNMSGB1.r04.med.va.gov> ??? Content-Type: text/plain; charset="us-ascii" That's interesting, I didn't realize gelatin is the water soluable protein of collagen. No experience with staining gelatin, but have you considered MassonTrichrome. It is used to differentiate collagen from smooth muscle... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Tuesday, February 24, 2015 12:48 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] gelatin You need to explain "treated tissue". Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope. If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. John Kiernan London, Canada = = = On 23/02/15, Yak-Nam Wang? wrote: > Hello, > > Does anyone know of a stain specific for gelatin? I would like to > distinguish between firbous collagen and gelatin in treated tissue. > > thank you > > Yak-Nam > > University of Washington > Seattle, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 24 Feb 2015 07:21:12 -0800 From: Paula Sicurello Subject: [Histonet] How dark is dark enough? To: HistoNet Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula? :-) ------------------------------ Message: 5 Date: Tue, 24 Feb 2015 07:30:19 -0800 From: Yak-Nam Wang Subject: Re: [Histonet] gelatin To: John Kiernan Cc: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Thank you for your e-mail. Apologies for not explaining "treated tissue". We treat the tissue with high intensity focused ultrasound. It can raise the temperature of tissue to boiling in a localized area (millimeter areas). I could use a biochemical assay for collagen and gelatin if we treat a large area, but with single lesions I was hoping I could visualize this. In some treated areas we are almost resulting in liquefaction of the tissue. I am interested to see if we are turning the collagen to gelatin in these areas and what part of the lesion this is happening. Thank you for your thoughts Yak-Nam On Mon, Feb 23, 2015 at 9:47 PM, John Kiernan wrote: > You need to explain "treated tissue". > > Gelatin is collagen that has been boiled until the protein has lost all > its fibrous nature and changed into a water-soluble protein. Gelatin is > made permanently insoluble by adequate formaldehyde fixation. It is stained > by anionic dyes (such as eosin in the H&E method), but it does not show as > fibres when you look at the section or smear through a microscope. > > If this doesn't answer your question, please explain your problem and > involve your boss in future email exchanges. > > *John Kiernan* > London, Canada > = = = > > On 23/02/15, *Yak-Nam Wang * wrote: > > Hello, > > Does anyone know of a stain specific for gelatin? I would like to > distinguish between firbous collagen and gelatin in treated tissue. > > thank you > > Yak-Nam > > University of Washington > Seattle, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 6 Date: Tue, 24 Feb 2015 15:37:09 +0000 (UTC) From: Rene J Buesa Subject: Re: [Histonet] How dark is dark enough? To: Paula Sicurello , ??? HistoNet ??? Message-ID: ??? <1238200618.5179661.1424792229766.JavaMail.yahoo@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 As long as your microscope has a good Hg source light, it is not really necessary to darken the room so much.?I used to have a very small room were the microscope was located but later on we moved the microscope to another area were we did not dim the light at all and the vision was OK.Everything depends on the circumstances and how comfortable?you are with the image.There are no rules?on this subject.Ren? J.? ? ? On Tuesday, February 24, 2015 10:21 AM, Paula Sicurello wrote: ? Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula? :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ------------------------------ Message: 7 Date: Tue, 24 Feb 2015 15:45:12 +0000 From: "Scott, Allison D" Subject: [Histonet] Creutzfeldt-Jakob? Disease To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset="us-ascii" Hello to all in histoland.? Does anyone have a procedure for handling creutzfeldt-jakob disease.? Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 8 Date: Tue, 24 Feb 2015 15:57:54 +0000 From: Debra Siena Subject: [Histonet] RE: Creutzfeldt-Jakob? Disease To: "Scott, Allison D" , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? ??? Content-Type: text/plain; charset="us-ascii" Hi Allison, I would suggest going to the CDC website and pulling from there, they should have the latest recommendations.? thanks Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Tuesday, February 24, 2015 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Creutzfeldt-Jakob Disease Hello to all in histoland.? Does anyone have a procedure for handling creutzfeldt-jakob disease.? Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 24 Feb 2015 16:06:37 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] How dark is dark enough? To: 'Paula Sicurello' , HistoNet ??? Message-ID: ??? <761E2B5697F795489C8710BCC72141FF367F6B5C@ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" If you are using an automated stainer the plexiglas cover that they all have will block UV light. For instance we run all our IF in a Dako stainer with the slightly tinted Plexiglas and have not had any problems.? If staining manually in? a tray, covering with something to block light is ok. On the other hand, I can't really say I've had a problem even with no light blocking. The incubations are usually so short it may not make a difference. Consider that the focused UV light in the fluorescence scope is thousands of times stronger than any overhead tube and it still takes a while for the signal to dim. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, February 24, 2015 7:21 AM To: HistoNet Subject: [Histonet] How dark is dark enough? Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula? :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 24 Feb 2015 11:41:41 -0500 From: "Roy, Ryan" Subject: [Histonet] CBG recycler To: "histonet@lists.utsouthwestern.edu" ??? Cc: "Hall, Kevin P." Message-ID: ??? <15F883394EAB744E99E1C7E1B98730490178A821D214@R04BYNMSGB1.r04.med.va.gov> ??? Content-Type: text/plain; charset="us-ascii" Hello histonet, Has anyone out there dealt with a busted Heat Resistor in a CBG reagent recycler. Any thoughts or insights appreciated. Thanks in advance, Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 135, Issue 25 ***************************************** From mundayscott <@t> gmavt.net Tue Feb 24 13:33:04 2015 From: mundayscott <@t> gmavt.net (scott munday) Date: Tue Feb 24 13:33:32 2015 Subject: [Histonet] Is your lab in need of a new microscope? Message-ID: *Is your lab in need of a new microscope? We have several Olympus BX40 and BX41 compound microscopes in stock! and offer a 1 year warranty on all scopes. * *All microscopes are fully refurbished and are priced at 40% off list. The scopes are serviced by an Authorized Olympus Service Tech before sold. Please Email or call with questions.* -- Scott Munday 90 Misha Lane Sanford, NC 27330 Phone: 919-775-5596 Fax: 919-776-9566 From Kimberly <@t> animalreferencepathology.com Tue Feb 24 15:29:39 2015 From: Kimberly <@t> animalreferencepathology.com (Kimberly Marshall) Date: Tue Feb 24 15:30:12 2015 Subject: [Histonet] Utah Society Message-ID: <1424813381530.59800@animalreferencepathology.com> ?Hello Histo folks. Are there any Utah Histo Tech subscribing or anyone with the information that Utah has a Histo society? Would really like info. Thanks in advance From rhworkman <@t> uro.com Tue Feb 24 15:33:06 2015 From: rhworkman <@t> uro.com (Renee H. Workman) Date: Tue Feb 24 15:33:50 2015 Subject: [Histonet] sections not sticking to charged slides Message-ID: Help, sections not sticking to charged slides. We use Mercedes Medical charged slides. Lately have been using sta-on but still have occasional problems especially during antigen retrieval. I need any suggestions. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. From Terra.Wineman <@t> novusint.com Tue Feb 24 15:43:47 2015 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Tue Feb 24 15:44:10 2015 Subject: [Histonet] RE: Utah Society In-Reply-To: <1424813381530.59800@animalreferencepathology.com> References: <1424813381530.59800@animalreferencepathology.com> Message-ID: <1EB8F245A303564EADF12AC7022FA74DD76CD773@novus-ex01> Utah is in NSH region 7 and your regional director is Jane Parr, Jane.parr@ucdenver.edu . I would contact her to double check. Terra Wineman, HTL (ASCP)CM Research Biologist, Nutritional Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Marshall Sent: Tuesday, February 24, 2015 3:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Utah Society ?Hello Histo folks. Are there any Utah Histo Tech subscribing or anyone with the information that Utah has a Histo society? Would really like info. Thanks in advance _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.Whitaker <@t> osumc.edu Tue Feb 24 15:47:16 2015 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Tue Feb 24 15:48:14 2015 Subject: [Histonet] slide folders for "double wide" slides Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E13C2A86A@EXMBOX-VP05.OSUMC.EDU> Hi All, What slide folders do any of you recommend for use with 2x3" slides (we use them for whole-mount prostates)? If you have some that work well, could you send me the ordering info? Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 From DSiena <@t> statlab.com Tue Feb 24 15:52:22 2015 From: DSiena <@t> statlab.com (Debra Siena) Date: Tue Feb 24 15:52:49 2015 Subject: [Histonet] RE: sections not sticking to charged slides In-Reply-To: References: Message-ID: Hi Renee, Hope you don't mind but I have had a lot of experience in this area. I worked for Ventana for 7 1/2 years as technical specialist and now as a Technical Support Manager and I know that getting sections to stick to slides can be a bit challenging especially during antigen retrieval. One of the rules when using positively charged slides is to not use any type of adhesive in the water bath, such as Sta-On only use distilled water. The use of such products actually works against you when trying to get the sections adhered to slides. Also I would say that when doing IHC, something to keep in mind is that adhesive properties of the slides vary over time, so age of the slides is a factor and also exposure to humidity and heat is another factor which can affect the adhesive properties of the slides. Make sure that you are not buying too many slides at any one time and that you go through them in no more than 6 months. Also if cutting controls and storing in a plastic box that can mean that the slides sit around for awhile before being used, so don't cut too many controls at one time. I would suggest drying the slides for at least 30 minutes to 1 hour at 60C and if you are getting trapped water under the section, you may need to dry longer to make sure that all the water is removed. If you would like to sample some of the StatLab slides that we feel are very good for IHC, please let myself or Kevin Collins know, we would be happy to sample them if you haven't already tried them. If I can help in any other way, please let me know. ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.767.3992 dsiena@statlab.com | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman Sent: Tuesday, February 24, 2015 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections not sticking to charged slides Help, sections not sticking to charged slides. We use Mercedes Medical charged slides. Lately have been using sta-on but still have occasional problems especially during antigen retrieval. I need any suggestions. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson <@t> pathology-associates.com Tue Feb 24 15:59:41 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Tue Feb 24 16:00:16 2015 Subject: [Histonet] RE: sections not sticking to charged slides In-Reply-To: References: Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CAB6@PAEXCH1.PathologyAssociates.local> HI Renee- I had a major problem in the past with tissue staining for IHC lifting off the slides no matter what I tried. It turned out that when we precut our controls and put them in the oven and then used them later for the patient tissue that the charge on the slide was altered. We currently still precut controls on control slides (we use Leica APEX) and then let them just air dry with no heat. We then use those slides when needed and add the patient tissue to the bottom and then put the slides in the oven (we use 70C for one hour) prior to IHC staining. The tissue lifting decreased dramatically- even on breast tissue. Be sure to pick up your controls from the wrong (label) end when picking up your controls and do not let the bottom half of the slide get into the waterbath so as to avoid "double-dipping" which can also cause tissue adherence problems. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman Sent: Tuesday, February 24, 2015 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections not sticking to charged slides Help, sections not sticking to charged slides. We use Mercedes Medical charged slides. Lately have been using sta-on but still have occasional problems especially during antigen retrieval. I need any suggestions. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From Richard.Cartun <@t> hhchealth.org Tue Feb 24 16:15:27 2015 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Tue Feb 24 16:15:37 2015 Subject: [Histonet] Prostate Biopsies Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3E0FF98C@HHCEXCHMB03.hhcsystem.org> Short of using a bar-coded tracking system, does anyone use "color-coded" formalin containers or cassettes for prostate biopsies (for the urology office staff) to confirm the patient's identity? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Timothy.Morken <@t> ucsf.edu Tue Feb 24 16:48:30 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Feb 24 16:48:38 2015 Subject: [Histonet] RE: slide folders for "double wide" slides In-Reply-To: <6B106EE8C8AAEF449DEA97921DEC11670E13C2A86A@EXMBOX-VP05.OSUMC.EDU> References: <6B106EE8C8AAEF449DEA97921DEC11670E13C2A86A@EXMBOX-VP05.OSUMC.EDU> Message-ID: <761E2B5697F795489C8710BCC72141FF367F6D8A@ex07.net.ucsf.edu> Hi Bonnie, we use some from Brain Research laboratories. They are cardboard flats without the dividers that are in the 20-slide types . They have 2x3 slide mailers as well. http://brainresearchlab.com/product-category/slide-storage/slide-folders/ Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Whitaker, Bonnie Sent: Tuesday, February 24, 2015 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide folders for "double wide" slides Hi All, What slide folders do any of you recommend for use with 2x3" slides (we use them for whole-mount prostates)? If you have some that work well, could you send me the ordering info? Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Feb 24 23:36:33 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Feb 24 23:36:45 2015 Subject: [Histonet] gelatin In-Reply-To: <7330ebc21362a.54ed5f59@uwo.ca> References: <73c0dcdb16c2f.54ec0d4d@uwo.ca> <7340bb0812783.54ec0d89@uwo.ca> <739091ff10f97.54ec0dc7@uwo.ca> <73908f9416392.54ec0e05@uwo.ca> <737080d3111c3.54ec0e43@uwo.ca> <7310982212109.54ec0e81@uwo.ca> <7380b38a112e6.54ec0ebf@uwo.ca> <73909cbc16334.54ec0efd@uwo.ca> <73c098ae13f0a.54ec0f3b@uwo.ca> <73c0dd6c107d0.54ec0f79@uwo.ca> <7290c2ec17867.54ec0fb7@uwo.ca> <73809e8f1288d.54ec0ff5@uwo.ca> <7390dcf9173d5.54ec1033@uwo.ca> <7390f10a164c2.54ec1071@uwo.ca> <7390897614c8f.54ebca36@uwo.ca> <7340e8811788b.54ed5c69@uwo.ca> <7340c94f17704.54ed5ca6@uwo.ca> <7340fa9813157.54ed5ce4@uwo.ca> <7380e48217c82.54ed5d22@uwo.ca> <73909ca61799a.54ed5d60@uwo.ca> <7270a71510be2.54ed5d9e@uwo.ca> <7340e2fb124bc.54ed5ddc@uwo.ca> <734088621247c.54ed5e1a@uwo.ca> <7310d04415c8b.54ed5e5a@uwo.ca> <7330cd1d13064.54ed5e98@uwo.ca> <73809c5b15b89.54ed5edc@uwo.ca> <7360dcba10300.54ed5f1a@uwo.ca> <7330ebc21362a.54ed5f59@uwo.ca> Message-ID: <7330ed7010ee5.54ed1911@uwo.ca> Dear ynwang@u.washington.edu. Boiling makes steam, with bubbles that greatly change tissue structure. Slow freezing is just as bad; the ice crystals make holes that deform and replace the tissue architecture. What are you tryng to find out? It has been known for 100+ years that boiling collagen makes gelatin, and further concentration makes traditional glue. You should involve your boss in future email exchanges. John Kiernan London, Canada = = = On 24/02/15, Yak-Nam Wang wrote: > Thank you for your e-mail. > > > Apologies for not explaining "treated tissue". We treat the tissue with high intensity focused ultrasound. It can raise the temperature of tissue to boiling in a localized area (millimeter areas). I could use a biochemical assay for collagen and gelatin if we treat a large area, but with single lesions I was hoping I could visualize this. In some treated areas we are almost resulting in liquefaction of the tissue. I am interested to see if we are turning the collagen to gelatin in these areas and what part of the lesion this is happening. You should involve your boss in future email exchanges. > > > > > > > > > > Thank you for your thoughts > > Yak-Nam > > > > On Mon, Feb 23, 2015 at 9:47 PM, John Kiernan wrote: > > > You need to explain "treated tissue". > > > > Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope. > > > > If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. > > > > John Kiernan > > London, Canada > > = = = > > On 23/02/15, Yak-Nam Wang wrote: > > > > > > > Hello, > > > > > > Does anyone know of a stain specific for gelatin? I would like to > > > distinguish between firbous collagen and gelatin in treated tissue. > > > > > > thank you > > > > > > Yak-Nam > > > > > > University of Washington > > > Seattle, WA > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > From a.prior <@t> tissueregenix.com Wed Feb 25 04:36:55 2015 From: a.prior <@t> tissueregenix.com (Andrew Prior) Date: Wed Feb 25 04:37:19 2015 Subject: [Histonet] gelatin Message-ID: Hi Yak-Nam, Have you thought of using Picrosirius Red staining? We use it to assess changes in the collagen fibres. Under polarised light, the collagen fibres exhibit birefringence (red/orange or green depending on fibre size) and the birefringence is lost/ becomes fainter as the collagen becomes degraded. You can boil spare tissue samples for different lengths of time to act as control/reference blocks for comparison. Hope that helps. Andrew Prior Histologist Tissue Regenix Group Heslington, York YO10 5NY E-mail: a.prior@tissueregenix.com Website: www.tissueregenix.com Message: 5 Date: Tue, 24 Feb 2015 07:30:19 -0800 From: Yak-Nam Wang > Subject: Re: [Histonet] gelatin To: John Kiernan > Cc: "histonet@lists.utsouthwestern.edu" > Message-ID: > Content-Type: text/plain; charset=ISO-8859-1 Thank you for your e-mail. Apologies for not explaining "treated tissue". We treat the tissue with high intensity focused ultrasound. It can raise the temperature of tissue to boiling in a localized area (millimeter areas). I could use a biochemical assay for collagen and gelatin if we treat a large area, but with single lesions I was hoping I could visualize this. In some treated areas we are almost resulting in liquefaction of the tissue. I am interested to see if we are turning the collagen to gelatin in these areas and what part of the lesion this is happening. Thank you for your thoughts Yak-Nam From DEBORAH_ELLENBURG <@t> bshsi.org Wed Feb 25 04:44:51 2015 From: DEBORAH_ELLENBURG <@t> bshsi.org (Ellenburg, Deborah) Date: Wed Feb 25 04:45:16 2015 Subject: [Histonet] RE: Prostate Biopsies In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3E0FF98C@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3E0FF98C@HHCEXCHMB03.hhcsystem.org> Message-ID: Our PA inks all prostate and breast core biopsy specimens with a different color ink and dictates the color of ink applied to the case in the gross dictation. Deborah Ellenburg, HT (ASCP) Histology Supervisor Bon Secours St. Francis Health System One St. Francis Drive Greenville, SC 29601 864-255-1585 (office) 864-444-8291 (work mobile) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Tuesday, February 24, 2015 5:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostate Biopsies Short of using a bar-coded tracking system, does anyone use "color-coded" formalin containers or cassettes for prostate biopsies (for the urology office staff) to confirm the patient's identity? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From mpence <@t> grhs.net Wed Feb 25 08:06:48 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Feb 25 08:07:10 2015 Subject: [Histonet] RE: Prostate Biopsies In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3E0FF98C@HHCEXCHMB03.hhcsystem.org> Message-ID: I tried the ink for our group and they hated it. Said it caused them problems reading????? I don't know why. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellenburg, Deborah Sent: Wednesday, February 25, 2015 4:46 AM To: Cartun, Richard; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Prostate Biopsies Our PA inks all prostate and breast core biopsy specimens with a different color ink and dictates the color of ink applied to the case in the gross dictation. Deborah Ellenburg, HT (ASCP) Histology Supervisor Bon Secours St. Francis Health System One St. Francis Drive Greenville, SC 29601 864-255-1585 (office) 864-444-8291 (work mobile) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Tuesday, February 24, 2015 5:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostate Biopsies Short of using a bar-coded tracking system, does anyone use "color-coded" formalin containers or cassettes for prostate biopsies (for the urology office staff) to confirm the patient's identity? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Wed Feb 25 08:18:00 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Wed Feb 25 08:18:40 2015 Subject: [Histonet] RE: Prostate Biopsies Message-ID: Okay, don?t laugh- we use colored glass beads in the cassette. Processing doesn?t affect them, same colored mark goes on the top right corner (either works) of the requisition, same colored bead is embedded in the top of the cassette at embedding, same colored pencil mark lines the top of the slides during sectioning. Some of the colored pencils change colors with staining, we use Crayola pencils with great success. Little glass beads are found at hobby lobby or other hobby stores. That works successfully and doesn?t take much time to do (inking is useful but takes forever, for us anyway). Let me know if you have other questions re: glass beads, we use all primary colors and black, brown, purple. We haven?t found a pink pencil that doesn?t change colors after staining though. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 2/25/15, 7:06 AM, "Mike Pence" wrote: >I tried the ink for our group and they hated it. Said it caused them >problems reading????? I don't know why. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Ellenburg, Deborah >Sent: Wednesday, February 25, 2015 4:46 AM >To: Cartun, Richard; histonet@lists.utsouthwestern.edu >Subject: [Histonet] RE: Prostate Biopsies > >Our PA inks all prostate and breast core biopsy specimens with a >different color ink and dictates the color of ink applied to the case in >the gross dictation. > >Deborah Ellenburg, HT (ASCP) >Histology Supervisor >Bon Secours St. Francis Health System >One St. Francis Drive >Greenville, SC 29601 >864-255-1585 (office) >864-444-8291 (work mobile) > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, >Richard >Sent: Tuesday, February 24, 2015 5:15 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Prostate Biopsies > >Short of using a bar-coded tracking system, does anyone use >"color-coded" formalin containers or cassettes for prostate biopsies (for >the urology office staff) to confirm the patient's identity? Thanks. > >Richard > >Richard W. Cartun, MS, PhD >Director, Histology & Immunopathology >Director, Biospecimen Collection Programs Assistant Director, Anatomic >Pathology Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 972-1596 >(860) 545-2204 Fax > > >This e-mail message, including any attachments, is for the sole use of >the intended recipient(s) and may contain confidential and privileged >information. Any unauthorized review, use, disclosure, or distribution is >prohibited. If you are not the intended recipient, or an employee or >agent responsible for delivering the message to the intended recipient, >please contact the sender by reply e-mail and destroy all copies of the >original message, including any attachments. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >__________________________________________________________________________ >______________________________ >The information in this communication is intended to be confidential to >the Individual(s) and/or Entity to whom it is addressed. >It may contain information of a Privileged and/or Confidential nature, >which is subject to Federal and/or State privacy regulations. >In the event that you are not the intended recipient or the agent of the >intended recipient, do not copy or use the information contained within >this communication, or allow it to be read, copied or utilized in any >manner, by any other person(s). Should this communication be received in >error, please notify the sender immediately either by response e-mail or >by phone, and permanently delete the original e-mail, attachment(s), and >any copies. >__________________________________________________________________________ >______________________________ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Wed Feb 25 10:36:41 2015 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Feb 25 10:37:09 2015 Subject: [Histonet] Prostate Biopsies In-Reply-To: <20150225143319.EEBEB2D021D@mail.pa-ucl.com> References: <20150225143319.EEBEB2D021D@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115996296@PEITHA.wad.pa-ucl.com> We do not have a process that occurs at the urology office. Our PA places a colored marker made from Histogel and marking ink into the cassettes and includes that information in the gross dictation. Nancy Schmitt MLT, HT(ASCP) __________________________________________________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Tuesday, February 24, 2015 5:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostate Biopsies Short of using a bar-coded tracking system, does anyone use "color-coded" formalin containers or cassettes for prostate biopsies (for the urology office staff) to confirm the patient's identity? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax From DWolfe <@t> capitalhealth.org Tue Feb 24 12:28:30 2015 From: DWolfe <@t> capitalhealth.org (Wolfe, Dee) Date: Wed Feb 25 10:45:28 2015 Subject: [Histonet] PowerPath vs. Softpath Message-ID: <3E0B56F0F06BDC4FAD1A867B9B39CFD04959CB9A@CHHMBX02.ad.chsnj.org> Hello all, We are starting the process of upgrading our PowerPath system (which we are very happy with) but before we can do that we have been asked to look at Softpath since the clinical lab is switching to Soft. I would welcome any comments or experiences with both systems that anyone can offer to help us with our decisions. Thanks in advance, Dee Wolfe Dee Wolfe MLT(ASCP), MT(HHS) Anatomic Pathology Coordinator Capital Health Regional Medical Center Laboratory dwolfe@capitalhealth.org 609-394-6434 Fax 609-278-5435 This electronic transmission and any attachments hereto contains material that is private and confidential in nature and may be protected by law. It is for the intended recipient only. Any other disclosures are unintended. If you have received this transmission, or a copy thereof, in error you are instructed to immediately and permanently delete this transmission and any attachments, and to notify the sender of such error by telephone at (609) 303-4000. Thank you for your cooperation and assistance. For Secure Email Instructions go to: http://www.chsmdconnect.com/postx From shive003 <@t> umn.edu Tue Feb 24 16:58:56 2015 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Feb 25 10:45:37 2015 Subject: [Histonet] CD68 and CD204 for dogs/pigs Message-ID: Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From liz <@t> premierlab.com Wed Feb 25 11:08:03 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Feb 25 11:08:10 2015 Subject: [Histonet] CD68 and CD204 for dogs/pigs In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79F17CDF@SBS2K8.premierlab.local> Jan MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Wed Feb 25 11:08:55 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Wed Feb 25 11:09:29 2015 Subject: [Histonet] Prostate Biopsies Message-ID: Histogel and marking ink, clever! I like that! Michael Ann On 2/25/15, 9:36 AM, "Nancy Schmitt" wrote: >We do not have a process that occurs at the urology office. >Our PA places a colored marker made from Histogel and marking ink into >the cassettes and includes that information in the gross dictation. > >Nancy Schmitt MLT, HT(ASCP) >__________________________________________________________________________ >________ > >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, >Richard >Sent: Tuesday, February 24, 2015 5:15 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Prostate Biopsies > >Short of using a bar-coded tracking system, does anyone use >"color-coded" formalin containers or cassettes for prostate biopsies (for >the urology office staff) to confirm the patient's identity? Thanks. > >Richard > >Richard W. Cartun, MS, PhD >Director, Histology & Immunopathology >Director, Biospecimen Collection Programs Assistant Director, Anatomic >Pathology Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 972-1596 >(860) 545-2204 Fax > > From TGoins <@t> mt.gov Wed Feb 25 11:19:14 2015 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Feb 25 11:20:12 2015 Subject: [Histonet] CD68 and CD204 for dogs/pigs In-Reply-To: References: Message-ID: I just received Cd204 from Biorbyt but have not validated a protocol. They are located in the UK but have a distributor in San Francisco so shipping is not outrageous. References to its application: The class A macrophage scavenger receptor CD204 is a useful immunohistochemical marker of canine histiocytic sarcoma. J Comp Pathol 2013 Feb 16;148(2-3):188-96. Epub 2012 Aug 16. Y Kato, M Murakami, Y Hoshino, T Mori, K Maruo, A Hirata, T L D R Nakagawa, T Yanai, H Sakaiand Immunocytochemical detection of the class A macrophage scavenger receptor CD204 using air-dried cytologic smears of canine histiocytic sarcoma Yuki Kato1, Risa Funato1, Akihiro Hirata, Mami Murakami, Takashi Mori, Koji Maruo, Tokuma Yanai and Hiroki Sakai Article first published online: 28 AUG 2014 If you or anyone has had success with this antibody, please let me know. Thanks, Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Tuesday, February 24, 2015 3:59 PM To: histonet Subject: [Histonet] CD68 and CD204 for dogs/pigs Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. Thanks much in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides <@t> eae.csic.es Wed Feb 25 11:55:49 2015 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Wed Feb 25 12:01:36 2015 Subject: [Histonet] CD68 and CD204 for dogs/pigs In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79F17CDF@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79F17CDF@SBS2K8.premierlab.local> Message-ID: <54EE0CA5.20909@eae.csic.es> Hi, MAC387 is a great marker but it detects less macrophages than CD68. In our experience (sheep and cattle), MAC387 is good for labelling macrophages that have recently reach the lesion, while those resident or long standing macrophages would be negative to MAC387 (but mostly positive to CD68, which is a more general marker for macs). No idea about CD204 though. Just in case this helps Regards Julio On 25/02/2015 18:08, Elizabeth Chlipala wrote: > Jan > > MAC387 is a marker that we have used successfully in canine and porcine, not entirely specific to macs but may work for your situation. > > LIz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > Sent: Tuesday, February 24, 2015 3:59 PM > To: histonet > Subject: [Histonet] CD68 and CD204 for dogs/pigs > > Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs (on FFPE tissue)? I don't seem to find much information on species cross-reactivity online. > > Thanks much in advance. > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melsmith <@t> udel.edu Wed Feb 25 15:09:12 2015 From: melsmith <@t> udel.edu (Melanie Smith) Date: Wed Feb 25 15:09:34 2015 Subject: [Histonet] Alkaline phosphatase staining in FFPE tissue Message-ID: Hello, I was wondering if anyone has had success in staining osteoblasts via alkaline phosphatase in decaled ffpe tissues? Currently using a sigma kit without success even with pre-incubating overnight with magnesium chloride. Thanks! Melanie -- Melanie Smith, MS University of Delaware melsmith@udel.edu From histotech <@t> imagesbyhopper.com Wed Feb 25 17:52:34 2015 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Feb 25 17:53:06 2015 Subject: [Histonet] RE: sections not sticking to charged slides In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CAB6@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CAB6@PAEXCH1.PathologyAssociates.local> Message-ID: <4CCACA7D-EDB9-4F16-8AD5-8E167DD24F3B@imagesbyhopper.com> We follow the same process as Jeff. Michelle On Feb 24, 2015, at 4:59 PM, Jeffrey Robinson wrote: HI Renee- I had a major problem in the past with tissue staining for IHC lifting off the slides no matter what I tried. It turned out that when we precut our controls and put them in the oven and then used them later for the patient tissue that the charge on the slide was altered. We currently still precut controls on control slides (we use Leica APEX) and then let them just air dry with no heat. We then use those slides when needed and add the patient tissue to the bottom and then put the slides in the oven (we use 70C for one hour) prior to IHC staining. The tissue lifting decreased dramatically- even on breast tissue. Be sure to pick up your controls from the wrong (label) end when picking up your controls and do not let the bottom half of the slide get into the waterbath so as to avoid "double-dipping" which can also cause tissue adherence problems. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman Sent: Tuesday, February 24, 2015 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections not sticking to charged slides Help, sections not sticking to charged slides. We use Mercedes Medical charged slides. Lately have been using sta-on but still have occasional problems especially during antigen retrieval. I need any suggestions. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu Feb 26 07:41:59 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Feb 26 07:42:07 2015 Subject: [Histonet] Oil Red O Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7FF189@GHSEXMBX1W8K1V.geisinger.edu> Hello Fellow Histonetters, We often get slides from another hospital for Oil Red O staining. On occasion, they send them at room temp. and until they get to us it's been a day or more. I'm worried that because they are unfixed the cells will be autolyzed and I don't want to charge the patient for a useless stain. I haven't been able to find any guidance on how long a frozen section can sit at room temp. and still be acceptable to stain for Oil Red O. Your thought and opinions please? Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From shive003 <@t> umn.edu Wed Feb 25 11:42:30 2015 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Feb 26 09:39:55 2015 Subject: [Histonet] CD68 and CD204 for dogs/pigs In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79F17CDF@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79F17CDF@SBS2K8.premierlab.local> Message-ID: Hi all, I do have MAC387 and it works great in dog and pig tissue. The PI is asking for additional CD markers to macrophages (CD68 and 204). I need them to work on dogs and pigs, but of course, there's not many vendors who list species cross-reactivities on their data sheets beyond the usual human and mouse. In my earlier trials years ago with CD68 (clone KP1), I didn't have success using it on dog tissue. Am looking for a different clone that will cross-react with dogs. Jan On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala wrote: > Jan > > MAC387 is a marker that we have used successfully in canine and porcine, > not entirely specific to macs but may work for your situation. > > LIz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > Sent: Tuesday, February 24, 2015 3:59 PM > To: histonet > Subject: [Histonet] CD68 and CD204 for dogs/pigs > > Can anyone recommend vendors for CD68 and CD204 that work on dogs and pigs > (on FFPE tissue)? I don't seem to find much information on species > cross-reactivity online. > > Thanks much in advance. > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From fbozkurt <@t> gmail.com Thu Feb 26 09:46:13 2015 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Thu Feb 26 09:46:17 2015 Subject: [Histonet] CD68 and CD204 for dogs/pigs In-Reply-To: References: <14E2C6176416974295479C64A11CB9AE019C79F17CDF@SBS2K8.premierlab.local> Message-ID: Hello Jan, I will stain ED1 (cd68) for you on dog tissue Friday. I hope it works. On Wed, Feb 25, 2015 at 7:42 PM, Jan Shivers wrote: > Hi all, > > I do have MAC387 and it works great in dog and pig tissue. The PI is > asking for additional CD markers to macrophages (CD68 and 204). I need > them to work on dogs and pigs, but of course, there's not many vendors who > list species cross-reactivities on their data sheets beyond the usual human > and mouse. > > In my earlier trials years ago with CD68 (clone KP1), I didn't have success > using it on dog tissue. Am looking for a different clone that will > cross-react with dogs. > > Jan > > On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala > wrote: > > > Jan > > > > MAC387 is a marker that we have used successfully in canine and porcine, > > not entirely specific to macs but may work for your situation. > > > > LIz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > liz@premierlab.com > > www.premierlab.com > > > > March 10, 2014 is Histotechnology Professionals Day > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > > Sent: Tuesday, February 24, 2015 3:59 PM > > To: histonet > > Subject: [Histonet] CD68 and CD204 for dogs/pigs > > > > Can anyone recommend vendors for CD68 and CD204 that work on dogs and > pigs > > (on FFPE tissue)? I don't seem to find much information on species > > cross-reactivity online. > > > > Thanks much in advance. > > > > -- > > Jan Shivers > > Senior Scientist > > IHC/Histology Section Head > > Pathology Teaching Program > > Veterinary Diagnostic Laboratory > > University of Minnesota > > 1333 Gortner Ave. > > St. Paul, MN 55108 > > 612-624-7297 > > shive003@umn.edu > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 From SSCALISE <@t> beaumont.edu Thu Feb 26 11:40:59 2015 From: SSCALISE <@t> beaumont.edu (Sharon Scalise) Date: Thu Feb 26 11:41:09 2015 Subject: [Histonet] RE: Expert opinion vs. second opinion Message-ID: <190D98228ADC1747BCE27019B78FD8F301273841@EXMAIL06.ms.beaumont.edu> I am looking for clarification about billing for expert opinion vs. second opinion. Can someone explain when and how each is used and billed so that I can pass this along to upper management. Thank you -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet Fatih BOZKURT Sent: Thursday, February 26, 2015 10:46 AM To: Jan Shivers; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CD68 and CD204 for dogs/pigs Hello Jan, I will stain ED1 (cd68) for you on dog tissue Friday. I hope it works. On Wed, Feb 25, 2015 at 7:42 PM, Jan Shivers wrote: > Hi all, > > I do have MAC387 and it works great in dog and pig tissue. The PI is > asking for additional CD markers to macrophages (CD68 and 204). I > need them to work on dogs and pigs, but of course, there's not many > vendors who list species cross-reactivities on their data sheets > beyond the usual human and mouse. > > In my earlier trials years ago with CD68 (clone KP1), I didn't have > success using it on dog tissue. Am looking for a different clone that > will cross-react with dogs. > > Jan > > On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala > > wrote: > > > Jan > > > > MAC387 is a marker that we have used successfully in canine and > > porcine, not entirely specific to macs but may work for your situation. > > > > LIz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > > Box 18592 Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > liz@premierlab.com > > www.premierlab.com > > > > March 10, 2014 is Histotechnology Professionals Day > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > > Sent: Tuesday, February 24, 2015 3:59 PM > > To: histonet > > Subject: [Histonet] CD68 and CD204 for dogs/pigs > > > > Can anyone recommend vendors for CD68 and CD204 that work on dogs > > and > pigs > > (on FFPE tissue)? I don't seem to find much information on species > > cross-reactivity online. > > > > Thanks much in advance. > > > > -- > > Jan Shivers > > Senior Scientist > > IHC/Histology Section Head > > Pathology Teaching Program > > Veterinary Diagnostic Laboratory > > University of Minnesota > > 1333 Gortner Ave. > > St. Paul, MN 55108 > > 612-624-7297 > > shive003@umn.edu > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lacie.Algeo <@t> providence.org Thu Feb 26 11:58:48 2015 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Thu Feb 26 11:59:17 2015 Subject: [Histonet] cilia for EM Message-ID: <24C4B3C167E5694887AB594C7602CE3A03BA82CF@WN35104.or.providence.org> Does anyone have a good technique/tool for cilia biopsy collection that we could share with our physicians. We have been having issues with poor quality specimens. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From bcooper <@t> chla.usc.edu Thu Feb 26 12:36:56 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Feb 26 12:37:02 2015 Subject: [Histonet] RE: cilia for EM In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A03BA82CF@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A03BA82CF@WN35104.or.providence.org> Message-ID: Will respondents please be sure to click "reply all" to this post? We're having the same issue here. Namely, remnants of the sample collection brush remain in the vial and damage our diamond knives. Any advice would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Thursday, February 26, 2015 9:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cilia for EM Does anyone have a good technique/tool for cilia biopsy collection that we could share with our physicians. We have been having issues with poor quality specimens. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From rjbuesa <@t> yahoo.com Thu Feb 26 12:58:27 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 26 12:58:32 2015 Subject: [Histonet] RE: Expert opinion vs. second opinion In-Reply-To: <190D98228ADC1747BCE27019B78FD8F301273841@EXMAIL06.ms.beaumont.edu> References: <190D98228ADC1747BCE27019B78FD8F301273841@EXMAIL06.ms.beaumont.edu> Message-ID: <42275889.639176.1424977107956.JavaMail.yahoo@mail.yahoo.com> I consider both as identical because you are not going to seek a?"second opinion" from somebody is not an expert.Asking another person with not real expertise?for an opinion?is nonsensical.Ren? J. On Thursday, February 26, 2015 12:41 PM, Sharon Scalise wrote: I am looking for clarification about billing for expert opinion vs. second opinion.? Can someone explain when and how each is used and billed so that I can pass this along to upper management. Thank you -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet Fatih BOZKURT Sent: Thursday, February 26, 2015 10:46 AM To: Jan Shivers; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CD68 and CD204 for dogs/pigs Hello Jan,? I will stain ED1 (cd68) for you on dog tissue Friday. I hope it works. On Wed, Feb 25, 2015 at 7:42 PM, Jan Shivers wrote: > Hi all, > > I do have MAC387 and it works great in dog and pig tissue.? The PI is > asking for additional CD markers to macrophages (CD68 and 204).? I > need them to work on dogs and pigs, but of course, there's not many > vendors who list species cross-reactivities on their data sheets > beyond the usual human and mouse. > > In my earlier trials years ago with CD68 (clone KP1), I didn't have > success using it on dog tissue.? Am looking for a different clone that > will cross-react with dogs. > > Jan > > On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala > > wrote: > > > Jan > > > > MAC387 is a marker that we have used successfully in canine and > > porcine, not entirely specific to macs but may work for your situation. > > > > LIz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > > Box 18592 Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > liz@premierlab.com > > www.premierlab.com > > > > March 10, 2014 is Histotechnology Professionals Day > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > > Sent: Tuesday, February 24, 2015 3:59 PM > > To: histonet > > Subject: [Histonet] CD68 and CD204 for dogs/pigs > > > > Can anyone recommend vendors for CD68 and CD204 that work on dogs > > and > pigs > > (on FFPE tissue)?? I don't seem to find much information on species > > cross-reactivity online. > > > > Thanks much in advance. > > > > -- > > Jan Shivers > > Senior Scientist > > IHC/Histology Section Head > > Pathology Teaching Program > > Veterinary Diagnostic Laboratory > > University of Minnesota > > 1333 Gortner Ave. > > St. Paul, MN? 55108 > > 612-624-7297 > > shive003@umn.edu > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN? 55108 > 612-624-7297 > shive003@umn.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhawkins <@t> UTMB.EDU Thu Feb 26 13:16:34 2015 From: hhawkins <@t> UTMB.EDU (Hawkins, Hal K.) Date: Thu Feb 26 13:16:40 2015 Subject: [Histonet] RE: cilia for EM In-Reply-To: References: <24C4B3C167E5694887AB594C7602CE3A03BA82CF@WN35104.or.providence.org>, Message-ID: <22624908330375439D6382C9F95093FF5818D111@GRMBX1.utmb.edu> My colleagues tell me a curette is the best instrument to use to collect ciliated cells from the nasal turbinates for electron microscopy. Presumably this would avoid the problem of bristles coming loose. I think this is the device described in this publication (please correct me if I'm wrong): Welch JE, Hogan MB, Wilson NW: Ten-year experience using a plastic, disposable curette for the diagnosis of primary ciliary dyskinesia. Ann Allergy Asthma Immunol 93(2):109, 2004. Hal Hawkins UTMB Gaveston ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cooper, Brian [bcooper@chla.usc.edu] Sent: Thursday, February 26, 2015 12:36 PM To: Algeo, Lacie A; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cilia for EM Will respondents please be sure to click "reply all" to this post? We're having the same issue here. Namely, remnants of the sample collection brush remain in the vial and damage our diamond knives. Any advice would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Thursday, February 26, 2015 9:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cilia for EM Does anyone have a good technique/tool for cilia biopsy collection that we could share with our physicians. We have been having issues with poor quality specimens. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oneilb <@t> wvuhealthcare.com Thu Feb 26 15:21:59 2015 From: oneilb <@t> wvuhealthcare.com (O'neil, Beth) Date: Thu Feb 26 15:22:06 2015 Subject: [Histonet] Ventana Ultra CD10 Message-ID: <3CEB8EBCF9C7A648B9694B5696462A7172642055@NT-EX2.wvuhs.com> We are in the last stages of contract negotiations for purchasing two Ventana Benchmark Ultras. During this time period we have been optimizing our current inventory of approximately 110 antibodies and validating before our current instrumentation is removed within the next two weeks. My Ventana application specialist is unable to successfully optimize CD10 (Ventana clone SP67) on the Ventana Benchmark Ultra. He had me send slides to their applications/troubleshooting lab but they told us they won't start working on it until next week and then it would take about a week for them to try and optimize it. We are in an urgent rush to get this antibody optimized and validated within the next two weeks since it is heavily requested by our Hemepaths. Would anyone be willing to share their Ultra protocols with me? Has anyone had similar experiences with Ventana being unsuccessful and having to send their slides to their applications lab for work up? Thank you for your help. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC oneilb@wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken <@t> ucsf.edu Thu Feb 26 16:12:14 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Feb 26 16:13:27 2015 Subject: [Histonet] RE: cilia for EM In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A03BA82CF@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A03BA82CF@WN35104.or.providence.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367F73CD@ex07.net.ucsf.edu> Our clinicians use a brush of some sort. Unfortunately I don't know the brand. But the bristles do not come off; they are pretty stout, are in a spiral pattern and each bristle has a knob at the end. We just gently scrape the cells off the brush into fixative in a petri dish and then pipet up and put in a several microtubes for centrifugation. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Thursday, February 26, 2015 9:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cilia for EM Does anyone have a good technique/tool for cilia biopsy collection that we could share with our physicians. We have been having issues with poor quality specimens. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From becky.garrison <@t> jax.ufl.edu Thu Feb 26 19:35:13 2015 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Thu Feb 26 19:35:20 2015 Subject: [Histonet] RE: Ventana Ultra CD10 In-Reply-To: <3CEB8EBCF9C7A648B9694B5696462A7172642055@NT-EX2.wvuhs.com> References: <3CEB8EBCF9C7A648B9694B5696462A7172642055@NT-EX2.wvuhs.com> Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F2DC8E3AD2@1B-ESX-MAIL3.umc.ufl.edu> For Ventana CD10, (SP67), we use the extended CC1 (92 minutes), antibody for 36 minutes and amplify on the Ultra. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'neil, Beth Sent: Thursday, February 26, 2015 4:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Ultra CD10 We are in the last stages of contract negotiations for purchasing two Ventana Benchmark Ultras. During this time period we have been optimizing our current inventory of approximately 110 antibodies and validating before our current instrumentation is removed within the next two weeks. My Ventana application specialist is unable to successfully optimize CD10 (Ventana clone SP67) on the Ventana Benchmark Ultra. He had me send slides to their applications/troubleshooting lab but they told us they won't start working on it until next week and then it would take about a week for them to try and optimize it. We are in an urgent rush to get this antibody optimized and validated within the next two weeks since it is heavily requested by our Hemepaths. Would anyone be willing to share their Ultra protocols with me? Has anyone had similar experiences with Ventana being unsuccessful and having to send their slides to their applications lab for work up? Thank you for your help. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC oneilb@wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Colleen_Herring <@t> bshsi.org Fri Feb 27 09:35:36 2015 From: Colleen_Herring <@t> bshsi.org (Herring, Colleen) Date: Fri Feb 27 09:35:45 2015 Subject: [Histonet] Frosted slides Message-ID: <3AE5857C7EC73F4E983F39C97DF811C018EF8875@EDC-EXMB4-01.ads.bshsi.com> Good Friday to all! We have just setup a Slide Mate printer and the slides we are using the frosted area is not quite large enough. What would be ideal is 2 1/2 cm across and 2 or 2 1/4 cm top to bottom, plain and plus in multi colors. any suggestions. Thanks ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From wbenton <@t> cua.md Fri Feb 27 10:17:13 2015 From: wbenton <@t> cua.md (Walter Benton) Date: Fri Feb 27 10:17:18 2015 Subject: [Histonet] RE: Frosted slides In-Reply-To: <3AE5857C7EC73F4E983F39C97DF811C018EF8875@EDC-EXMB4-01.ads.bshsi.com> References: <3AE5857C7EC73F4E983F39C97DF811C018EF8875@EDC-EXMB4-01.ads.bshsi.com> Message-ID: <0B8979A204680A42B93A52B486088CD94239439348@CUAEXH1.GCU-MD.local> Leica/Surgipath Slides X-tra Thermal works well. 3800090 is white and there are other colors as well. Avantik has a slide as well, but I have not tried their slide. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen Sent: Friday, February 27, 2015 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Frosted slides Good Friday to all! We have just setup a Slide Mate printer and the slides we are using the frosted area is not quite large enough. What would be ideal is 2 1/2 cm across and 2 or 2 1/4 cm top to bottom, plain and plus in multi colors. any suggestions. Thanks ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From tejohnson <@t> genoptix.com Fri Feb 27 12:40:25 2015 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Fri Feb 27 12:40:34 2015 Subject: [Histonet] Re: Frosted slides Message-ID: <43963a03b96143e0ad30730d9d52461c@PHUSCB-SP37MB04.genoptix.org> Use a smaller font? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From GauchV <@t> mail.amc.edu Fri Feb 27 13:18:13 2015 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Fri Feb 27 13:18:18 2015 Subject: [Histonet] Automated Embedding Centers Message-ID: Hi, Is anyone out there using automated embedding centers and, if so, what brand ? Any pros/cons ? Thank you, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From Ryan.Roy <@t> va.gov Fri Feb 27 13:41:51 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Fri Feb 27 13:42:28 2015 Subject: [Histonet] CBG recyler and recycled Formalin Message-ID: <15F883394EAB744E99E1C7E1B98730490178BF4ECA01@R04BYNMSGB1.r04.med.va.gov> Hello, We are getting a new CBG that recycles xylene , alcohol, and formalin. We purchase buffered formalin? Does anyone know if after recycling the recycled formalin would or would not need be re-buffered?? Thanks in advance, Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opions of the VA. From pwnoyce <@t> gmail.com Fri Feb 27 17:56:05 2015 From: pwnoyce <@t> gmail.com (Peter Noyce) Date: Fri Feb 27 17:54:34 2015 Subject: [Histonet] squash mounts for mitotic index count Message-ID: <000001d052e8$f5d87b80$e1897280$@gmail.com> Could anyone give me some good references as to how to best prepare this mount. Peter Noyce PhD student From gu.lang <@t> gmx.at Sat Feb 28 01:55:47 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Feb 28 01:56:01 2015 Subject: AW: [Histonet] RE: Expert opinion vs. second opinion In-Reply-To: <190D98228ADC1747BCE27019B78FD8F301273841@EXMAIL06.ms.beaumont.edu> References: <190D98228ADC1747BCE27019B78FD8F301273841@EXMAIL06.ms.beaumont.edu> Message-ID: <002b01d0532b$fb488f40$f1d9adc0$@gmx.at> Hi, I think the second opinion can be from your workmate next door, but the expert's opinion is usually from a specialist in his/her field. Usually the slides are sent out to another specialist facility. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sharon Scalise Gesendet: Donnerstag, 26. Februar 2015 18:41 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Expert opinion vs. second opinion I am looking for clarification about billing for expert opinion vs. second opinion. Can someone explain when and how each is used and billed so that I can pass this along to upper management. Thank you -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mehmet Fatih BOZKURT Sent: Thursday, February 26, 2015 10:46 AM To: Jan Shivers; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CD68 and CD204 for dogs/pigs Hello Jan, I will stain ED1 (cd68) for you on dog tissue Friday. I hope it works. On Wed, Feb 25, 2015 at 7:42 PM, Jan Shivers wrote: > Hi all, > > I do have MAC387 and it works great in dog and pig tissue. The PI is > asking for additional CD markers to macrophages (CD68 and 204). I > need them to work on dogs and pigs, but of course, there's not many > vendors who list species cross-reactivities on their data sheets > beyond the usual human and mouse. > > In my earlier trials years ago with CD68 (clone KP1), I didn't have > success using it on dog tissue. Am looking for a different clone that > will cross-react with dogs. > > Jan > > On Wed, Feb 25, 2015 at 11:08 AM, Elizabeth Chlipala > > wrote: > > > Jan > > > > MAC387 is a marker that we have used successfully in canine and > > porcine, not entirely specific to macs but may work for your situation. > > > > LIz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > > Box 18592 Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > liz@premierlab.com > > www.premierlab.com > > > > March 10, 2014 is Histotechnology Professionals Day > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > > Sent: Tuesday, February 24, 2015 3:59 PM > > To: histonet > > Subject: [Histonet] CD68 and CD204 for dogs/pigs > > > > Can anyone recommend vendors for CD68 and CD204 that work on dogs > > and > pigs > > (on FFPE tissue)? I don't seem to find much information on species > > cross-reactivity online. > > > > Thanks much in advance. > > > > -- > > Jan Shivers > > Senior Scientist > > IHC/Histology Section Head > > Pathology Teaching Program > > Veterinary Diagnostic Laboratory > > University of Minnesota > > 1333 Gortner Ave. > > St. Paul, MN 55108 > > 612-624-7297 > > shive003@umn.edu > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paintedsplashes <@t> yahoo.com Sat Feb 28 07:27:10 2015 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Sat Feb 28 07:27:14 2015 Subject: [Histonet] Positions at Stanford Message-ID: <161458616.247876.1425130030294.JavaMail.yahoo@mail.yahoo.com> Stanford Histology Services has a few great positions open and we are looking for some positive, enthusiastic Histotechs to joins us. ?Check out the list at Stanford Health Care careers. ?Feel free to contact me if you have any questions. Jeanne Clark ?Histology Services Supervisorjeclark@stanfordhealthcare.org From jfray80 <@t> hotmail.com Sat Feb 28 08:39:54 2015 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Sat Feb 28 08:39:59 2015 Subject: [Histonet] Automatic embedding center Message-ID: I would like some feedback on Sakura's Automatic embedding center for those who have one . I do not feel it would be a good fit for our lab due to many types and sizes of animal tissue . Please send me your opinions . Thanks From jfray80 <@t> hotmail.com Sat Feb 28 08:51:52 2015 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Sat Feb 28 08:51:57 2015 Subject: [Histonet] Histology Position Message-ID: Abaxis Reference Lab in Olathe , Kansas has an opening for a histotechnician position . This is an early morning first shift position . The hours will be 3:00 am to 11:30 am or 4:00 am to 12:30 . This position requires good Embedding and Microtomy skills . Please only skilled and serious applicants can send there Resume to josephfrazee@abaxis.com . From jqb7 <@t> cdc.gov Sat Feb 28 09:00:02 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Sat Feb 28 09:00:18 2015 Subject: [Histonet] Automatic embedding center In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B78137EEEEF1@EMBX-CLFT4.cdc.gov> Are you asking about the AutoTEC instrument? We have one and I would be glad to share info. Jeanine Sanders CDC Atlanta ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of JOSEPH FRAZEE [jfray80@hotmail.com] Sent: Saturday, February 28, 2015 9:39 AM To: Histonet Server Subject: [Histonet] Automatic embedding center I would like some feedback on Sakura's Automatic embedding center for those who have one . I do not feel it would be a good fit for our lab due to many types and sizes of animal tissue . Please send me your opinions . Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paintedsplashes <@t> yahoo.com Sat Feb 28 13:33:22 2015 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Sat Feb 28 13:33:30 2015 Subject: [Histonet] Stanford contact Message-ID: Email is jeclark@stanfordhealthcare.org Sent from my iPhone From kdwyer3322 <@t> aol.com Sat Feb 28 15:23:27 2015 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sat Feb 28 15:23:31 2015 Subject: [Histonet] ONLY " 20 DAYS" UNTIL THE "Texas Histology: A Wealth of Knowledge" Texas Society for Histotechnology Annual Symposium/Convention Dallas/Plano Marriott at Legacy TownCenter,Plano, Texas-March 20-22, 2015 In-Reply-To: <8D20B830DB668C2-610-5BE8@webmail-vm130.sysops.aol.com> References: <8D20B830DB668C2-610-5BE8@webmail-vm130.sysops.aol.com> Message-ID: <14bd2129111-4c72-ca71@webprd-a63.mail.aol.com> Hi Histonetters! Its not too late to sign up! The Texas Society for Histotechnology will be holding the 2015 S/C in Plano Texas. If you are interested in a electronic version of the program please send me a e-mail via this communication. It is not too late to sign up. We would love to have you join us this year! Regards, Kathy Dwyer txsh.org Take a look at the great symposiums and workshop we have to offer! Take Control of Your Controls- Heather Renko Understanding the Business of Pathology and How It Affects Your AP Laboratory- Loretta Sayles The Science of Fixation and Processing- Jan Minshew H. Pylori: Special Staining Methods and Troubleshooting - Ranna Mehta/ Debbie Siena Cool Tips for Cryostat Users - Jan Minshew Resume Wriitng and Interview Preparation ? JanaE Mitchell/ Amy Plewinski Optimization of Microtomy - Past, Present and the 21st Century -H. Skip. Brown Unmasking IHC and Molecular Techniques ? Brent Hart A Pratical Approach For The Histological Evaluation of Underminerlized Bone, synthetic Biomaterial and Medical Devie Implants ? Jack Ratliff A Critical Component of Autopy Pathology ? Shemeika Johnson From Mouse to Microscope - Jennifer Johnson Understanding the Characteristics of Stains and Dyes and Their Importance in the Histology Laboratory ? Debbie Siena/ Gary Weiderhold Guidelines for Studing Special Stains in preparation for the HT Examination ? Rayan Gonzalez/Lin Bustamante Capital Budgeting - Olga Kochar Streamling the Path Line ? Kelsi Currier/Carol BethTaylor /Ary Franklin Introduction to ISH and Case Studies ? Heather Renko The Pathologist Said: "I have Achromotyfil" and You Said" What?Is That" ? Pamela Marcum Quality Management- Yesterday, Today and Tomorrow-Olga Kochar Fundamental IHC Technique and Theories of In situ Hybridization with a Comparison to Immunohistochemistry-Theresa Burchette Oh My! What Does CLIA and CAP Want During and After A Inspection ? Debbie Siena/Kathy Dwyer Technological Advancements in Microtomy:A Non-Contact Alternative to Conventional Histology Equipment and Techniques- Jack Ratliff Leadership: Theory to Application ? Jennifer Nelson From rsrichmond <@t> gmail.com Sat Feb 28 15:29:35 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Feb 28 15:29:37 2015 Subject: [Histonet] Re: CBG recyler and recycled Formalin Message-ID: Ryan Roy HTL (ASCP) in Manchester NH asks: >>We are getting a new CBG that recycles xylene , alcohol, and formalin. We purchase buffered formalin. Does anyone know if after recycling the recycled formalin would or would not need be re-buffered?<< If you distill buffered formalin, the formaldehyde is going to distill over, but not the buffer phosphate, which will remain in the still pot. I suppose you can buy phosphate mixtures to make Lillie's neutral buffered formalin anew, using your recycled formaldehyde. I think you also have to measure the concentration of formalehyde in the distillate, and dilute accordingly. Bob Richmond Samurai Pathologist Maryville TN From jqb7 <@t> cdc.gov Sat Feb 28 15:36:24 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Sat Feb 28 15:36:41 2015 Subject: [Histonet] recyclers Message-ID: <3B2CD438E1628A41BD687E98B963B78137EEF1B6@EMBX-CLFT4.cdc.gov> Hello, Curious as to what the best, walk-away (i.e. easy) recycler is. Presently we only recycle xylene and alcohol. We don't mind 2 separate units if that simplifies things. Thanks, Jeanine Sanders CDC Atlanta