From Michael.Baker at cchmc.org Sat Aug 1 12:14:13 2015 From: Michael.Baker at cchmc.org (Baker, Michael) Date: Sat, 1 Aug 2015 17:14:13 +0000 Subject: [Histonet] Ventana H. pylori antibody In-Reply-To: References: Message-ID: Hello Julia, at Cincinnati Children?s we use a Ventana BenchMark Ultra for immunohistochemistry, and the antibody we use, which is extremely clean, is the Ventana rabbit monoclonal (SP48), catalog number 790 1014. Michael. ---- Michael Baker, M.D. CCHMC Pathology T (513) 636-4261 F (513) 636-3924 > On Aug 1, 2015, at 1:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > Date: Fri, 31 Jul 2015 19:56:27 +0000 > From: "Cates, Julia" > Subject: Re: [Histonet] Ventana H.Pylori antibody From PREISZNE at mail.etsu.edu Sun Aug 2 12:27:55 2015 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Sun, 2 Aug 2015 17:27:55 +0000 Subject: [Histonet] desperately in need of human alpha-synuclein positive control Message-ID: Hi, anybody heard about a place where I can buy human alpha-synuclein IHC positive control slides? Or anybody can spare some? Or brains with Lewy bodies? I can offer other control blocks you might need. Thanks, Hanna Preiszner ETSU/QCOM From Michael.LaFriniere at ccplab.com Mon Aug 3 06:32:54 2015 From: Michael.LaFriniere at ccplab.com (Michael LaFriniere) Date: Mon, 3 Aug 2015 11:32:54 +0000 Subject: [Histonet] Position Maryland Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2973E975@AHCMSASEXCH02.my.ahc.local> Our laboratory has Full Time Supervisory HT (ASCP) job position opening for right candidate in Silver Spring Maryland, large independent AP/lab with hospitals, surgery centers and physician office clientele. Further information please contact me directly. **************Not interested in job placement companies, head hunters or placement service calls or contacts ******* Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041?Bournefield Way, Suite?A . Silver Spring, MD 20904?? P:?240.471.3427?. F:?240.471.3401 . Cell 410-940-8844 michael.lafriniere at ccplab.com From bakevictoria at gmail.com Mon Aug 3 07:12:14 2015 From: bakevictoria at gmail.com (Victoria Baker) Date: Mon, 3 Aug 2015 08:12:14 -0400 Subject: [Histonet] Breast fixation In-Reply-To: References: Message-ID: Karen We take ours off on Sunday. The tissue processor is set up with 6 hours of fixation the minimum time. We have VIP 5's so on Friday the tissue goes on a delay run that is set to come out of formalin at 12:30 AM Sunday morning. The end of the process is at 9 AM Sunday morning. Just take the tissue off and let it solidify. When your next tech comes in they can put the cassette in the embedding center to heat up again and embed it. You will follow your same protocol and record your times accordingly. It might mean having to ask someone to come in for the 5 minutes it takes to do this but it works. If needed you can adjust your times to fit your schedule for someone being available to take them off. I hope this will help. Vikki On Jul 29, 2015 11:33 AM, "Heckford, Karen - SMMC-SF via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Good Morning, > I have a question about breast fixation. I am in a little bit of a > pickle with the 6-72 hour rule for the fixation on breast tissue. Friday > I am getting 2 breast cases in the afternoon and both will not have the > required minimum 6 hour formalin fixation for my per diem to cut early > Saturday morning. He will not be able to make it in again until Monday > night. The tissue will be about 3-4 hours (this includes time on the > processor) over the 72 hour maximum. Does anyone have any suggestions > on what can be done? We are a one person show here. > > Thanks, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > > Caution: This email message, including all content and > attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY > PRIVILEGED. The information contained in this email message is intended > only for the use of the recipient(s) named above. If the reader of this > message is not the intended recipient or an agent responsible for > delivering it to the intended recipient, you have received this document in > error. Any further review, dissemination, distribution, or copying of this > message is strictly prohibited. If you have received this communication in > error, please notify us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CDavis at che-east.org Mon Aug 3 07:59:41 2015 From: CDavis at che-east.org (Davis, Cassie) Date: Mon, 3 Aug 2015 08:59:41 -0400 Subject: [Histonet] Cerner Computer System Message-ID: Good Morning Histonet, I am hoping to touch base with anybody using Cerner. We have just been told we are switching and are wondering if it works with CoPath and Vantage. Any input would be appreciated. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From relia1 at earthlink.net Mon Aug 3 10:26:05 2015 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 3 Aug 2015 11:26:05 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors - 8-3-2015 Message-ID: <006c01d0ce00$b7f87660$27e96320$@earthlink.net> Hi Histonetters!! What would make the perfect management position? Is it the location? Perhaps the type of lab? How about the size of the staff/lab? Maybe it's the Hours? The Money and/or benefits? Could it be that a particular situation is the next logical step in your career? More than likely the answer is all of the above in different degrees. That is why I am contacting you with this special bulletin. I am getting more and more management opportunities every day and I wanted to touch base and let you know. Your next opportunity might be just around the corner and it could be right here with me!! If you are looking for a position right now please contact me right away. We can talk about my current positions OR about a customized search on your behalf. If you aren't looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, shoot me an email at relia1 at earthlink.net or call me toll free at 866-607-3542 and let me know!! Here is a list of my current managerial opportunities: Anatomic Pathology Manager - Hammond, IN Histology Supervisor Macon, GA Histology Supervisor - Dallas, TX Histology Supervisor - Flagstaff, AZ IHC Supervisor - Long Island, NY IHC Supervisor - Waynesboro, VA Lead Histotech/Supervisor - Great Falls, MT Lead Histotech/Supervisor - Waco, TX Lead Histotech - Atlanta, GA All of these clients offer autonomy in your position and histology staff eager to welcome their new manager. I really appreciate you taking time out of your busy day to read my e-mail Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From cjohnson at nmda.nmsu.edu Mon Aug 3 11:40:37 2015 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Mon, 3 Aug 2015 16:40:37 +0000 Subject: [Histonet] Handline paraffin Message-ID: This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From rjbuesa at yahoo.com Mon Aug 3 11:52:22 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 3 Aug 2015 16:52:22 +0000 (UTC) Subject: [Histonet] Handline paraffin In-Reply-To: References: Message-ID: <133646119.1028443.1438620742841.JavaMail.yahoo@mail.yahoo.com> It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or "irritating" substance. It is just an oil of high molecular weight (mineral oil)Ren? On Monday, August 3, 2015 12:48 PM, "Johnson, Carole via Histonet" wrote: This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree at uwhealth.org Mon Aug 3 14:35:24 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Mon, 3 Aug 2015 19:35:24 +0000 Subject: [Histonet] Glutamine Synthetase Message-ID: <77DD817201982748BC67D7960F2F76AF150167@UWHC-MBX12.uwhis.hosp.wisc.edu> Good afternoon, Our lab is looking for a reference lab to send FFPE slides for this antibody. The last place we sent them to was unsatisfactory. Thank you in advance, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From subash.govender at uct.ac.za Mon Aug 3 15:37:59 2015 From: subash.govender at uct.ac.za (Subash Govender) Date: Mon, 3 Aug 2015 20:37:59 +0000 Subject: [Histonet] Histonet Digest, Vol 141, Issue 1 In-Reply-To: References: Message-ID: <32CD6307BAB24F4DAD1C6E6B3E8AA223F43B5ECA@SRVWINEXC001.wf.uct.ac.za> -------- Original Message -------- Subject: Histonet Digest, Vol 141, Issue 1 From: histonet-request at lists.utsouthwestern.edu To: histonet at lists.utsouthwestern.edu CC: Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Ventana H.Pylori antibody (White, Marcia) 2. (no subject) (Teresa Harris) 3. P16 (kalkhenaizi .) 4. Re: Breast fixation (Cartun, Richard) 5. Re: Ventana H.Pylori antibody (Cates, Julia) ---------------------------------------------------------------------- Message: 1 Date: Fri, 31 Jul 2015 17:09:00 +0000 From: "White, Marcia" To: "'Jeffrey Robinson'" , "Cates, Julia" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Ventana H.Pylori antibody Message-ID: <8B4B5BF3FCB6D24CBEAA63ACA5FDAD3B51490B6F at MHSEXMB04.mhs.net> Content-Type: text/plain; charset="us-ascii" We also are having the same problem with the Ventana H.Pylori antibody on our Ultras, I have tried the CellMarque product in the past and as mentioned still not ideal. Will try the Biocare product and see if the results improve for us also. Marcia M White Pathology Manager Memorial Regional Hospital 954-265-5371-office 954-967-7627-fax MWhite at mhs.net -----Original Message----- From: Jeffrey Robinson [mailto:JRobinson at pathology-associates.com] Sent: Thursday, July 30, 2015 1:06 PM To: Cates, Julia; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana H.Pylori antibody Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his "go to" stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Cates, Julia via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 30, 2015 8:30 AM To: histonet-bounces at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana H.Pylori antibody Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. ------------------------------ Message: 2 Date: Fri, 31 Jul 2015 13:18:00 -0400 From: Teresa Harris To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] (no subject) Message-ID: Content-Type: text/plain; charset="us-ascii" Yes Sent from my iPhone ------------------------------ Message: 3 Date: Fri, 31 Jul 2015 22:14:43 +0300 From: "kalkhenaizi ." To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] P16 Message-ID: Content-Type: text/plain; charset=UTF-8 Hello Histonetters, Happy Friday! I need p16 antibody but not sure which vendor provide reliable antibody. I'd appreciate any recommendation. I also need melanoma and prostate ca control blocks, I can exchange with the once I have. Thank you and have a nice weekend. Kadhem Kadhem Alkhenaizi, MS HTL (ASCP) MB, QIHC Lab Manager ExpressMed Labs ------------------------------ Message: 4 Date: Fri, 31 Jul 2015 19:28:44 +0000 From: "Cartun, Richard" To: "Heckford, Karen - SMMC-SF" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Breast fixation Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B01855A at HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" There are several studies (one listed below) that have addressed the issue of fixation past the "recommended" 72 hour maximum. In my opinion, the science behind these fixation recommendations is "weak" at best. In my own experience, I have seen no problem whatsoever going well past 72 hours of fixation. The majority of breast cases will show internal positive controls for ER and PR to validate that the tissue is satisfactory for testing. Remember, working with your pathologist(s) you can perform your own validation on-site for extended fixation time. The real culprit for poor ER, PR, and HER2 IHC results is "inadequate" fixation. Reference: Tong LC, Nelson N, Tsourigiannis J, et al.: The effect of prolonged fixation on the IHC evaluation of ER, PR, and HER2 expression in invasive breast cancer: A prospective study. Am J Surg Pathol 2011;35:545-552. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 29, 2015 11:02 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Breast fixation Good Morning, I have a question about breast fixation. I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue. Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. He will not be able to make it in again until Monday night. The tissue will be about 3-4 hours (this includes time on the processor) over the 72 hour maximum. Does anyone have any suggestions on what can be done? We are a one person show here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 5 Date: Fri, 31 Jul 2015 19:56:27 +0000 From: "Cates, Julia" To: "histonet at lists.utsouthwestern.edu" , "histonet-bounces at lists.utsouthwestern.edu" Subject: Re: [Histonet] Ventana H.Pylori antibody Message-ID: Content-Type: text/plain; charset="iso-8859-1" Wow! Thank you all so much for your responses. They have been very helpful; it's so great to have a community of professionals that are so willing to give of their knowledge. Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole?use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. Message: 1 Date: Thu, 30 Jul 2015 17:06:04 +0000 From: Jeffrey Robinson To: "Cates, Julia" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Ventana H.Pylori antibody Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26 at PAEXCH1.PathologyAssociates.local> Content-Type: text/plain; charset="us-ascii" Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his "go to" stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Cates, Julia via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 30, 2015 8:30 AM To: histonet-bounces at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana H.Pylori antibody Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. _______________________________________________ ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 141, Issue 1 **************************************** ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. From jvickroy at SpringfieldClinic.com Mon Aug 3 16:13:17 2015 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Mon, 3 Aug 2015 21:13:17 +0000 Subject: [Histonet] Average number of slides reviewed by a surgical pathologist Message-ID: <9B1A1501A800064397369BD8072E6BCA06435B56@E2K10DB.springfieldclinic.com> I realize this is a question that may be impossible to answer given all of the variables in pathology labs, specimens, physicians, etc. However here goes: For those that work in a lab that primarily does biopsies ( mainly gi biopsies) what is the average number of slides that a pathologist reads each day. I have looked online for parameters recommended however I haven't had a whole lot of luck. There are plenty of figures on numbers of blocks and slides for histotechs but just wondered if anyone was using any either national benchmarks or local practices. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From rjbuesa at yahoo.com Mon Aug 3 17:01:34 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 3 Aug 2015 22:01:34 +0000 (UTC) Subject: [Histonet] Average number of slides reviewed by a surgical pathologist In-Reply-To: <9B1A1501A800064397369BD8072E6BCA06435B56@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA06435B56@E2K10DB.springfieldclinic.com> Message-ID: <2089577645.1289589.1438639294087.JavaMail.yahoo@mail.yahoo.com> Please go to: http://www.histosearch.com/rene.htmland download my article on "Staffing". The answer to your question is there.Ren? On Monday, August 3, 2015 5:20 PM, "Vickroy, James via Histonet" wrote: I realize this is a question that may be impossible to answer given all of the variables in pathology labs, specimens, physicians, etc.? However here goes:? For those that work in a lab that primarily does biopsies ( mainly gi biopsies) what is the average number of slides that a pathologist reads each day.? ? I have looked online for parameters recommended however I haven't had a whole lot of luck.? There are plenty of figures on numbers of blocks and slides for histotechs but just wondered if anyone was using any either national benchmarks or local practices. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epeters2 at gmu.edu Mon Aug 3 21:03:00 2015 From: epeters2 at gmu.edu (Esther C Peters) Date: Tue, 4 Aug 2015 02:03:00 +0000 Subject: [Histonet] Handline paraffin In-Reply-To: <133646119.1028443.1438620742841.JavaMail.yahoo@mail.yahoo.com> References: , <133646119.1028443.1438620742841.JavaMail.yahoo@mail.yahoo.com> Message-ID: However, I would be concerned if using Paraplast Plus (+) because it contains dimethyl sulfoxide (DMSO) to infiltrate the tissue faster. It can be absorbed through bare skin and you get a garlic taste in your mouth, making you wonder what else was absorbed into your body (DMSO is used for transdermal drug administration). We wear gloves when working with molten Paraplast +, but not for microtomy of the solidified blocks. Esther Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2 at gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: Rene J Buesa via Histonet Sent: Monday, August 3, 2015 12:52 PM To: Johnson, Carole; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Handline paraffin It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or "irritating" substance. It is just an oil of high molecular weight (mineral oil)Ren? On Monday, August 3, 2015 12:48 PM, "Johnson, Carole via Histonet" wrote: This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rascal_40 at hotmail.com Mon Aug 3 21:03:17 2015 From: rascal_40 at hotmail.com (Karen Stephanson) Date: Mon, 3 Aug 2015 20:03:17 -0600 Subject: [Histonet] Handline paraffin Message-ID: Is there any written documentation? On Aug 3, 2015 11:10 AM, Rene J Buesa via Histonet wrote: It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or "irritating" substance. It is just an oil of high molecular weight (mineral oil)Ren? On Monday, August 3, 2015 12:48 PM, "Johnson, Carole via Histonet" wrote: This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Mon Aug 3 22:46:58 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Mon, 03 Aug 2015 22:46:58 -0500 Subject: [Histonet] Handling paraffin In-Reply-To: <72a0a575c0b8.55c03578@uwo.ca> References: <72f0f113a452.55c02c8a@uwo.ca> <72f0a118e50a.55c02cc7@uwo.ca> <72f0ab1990d0.55c02d07@uwo.ca> <72f09c91b74c.55c02d45@uwo.ca> <73508020b0b6.55c02d83@uwo.ca> <735093a7e927.55c02dc1@uwo.ca> <72909393996b.55c02e3b@uwo.ca> <72a08ab3f9d7.55c02e7a@uwo.ca> <730081a8d41d.55c02eb8@uwo.ca> <7350a056d949.55c02ef5@uwo.ca> <73509fdaed7d.55c02f34@uwo.ca> <73509badb6f8.55c02fae@uwo.ca> <72f085c7bdd9.55c02fec@uwo.ca> <73009e0ded83.55c0302a@uwo.ca> <72a0bd418874.55c03068@uwo.ca> <7300acd2e412.55c030a6@uwo.ca> <7390af5fbf54.55c030e4@uwo.ca> <7390cb9be5de.55c03122@uwo.ca> <7340a3c8fa7a.55c03160@uwo.ca> <7240b8a59fd9.55c03216@uwo.ca> <72f0943ad86d.55c03254@uwo.ca> <72f09806c9cf.55c03292@uwo.ca> <7340dae1e8d6.55c032d0@uwo.ca> <72f0e32ddf5d.55c0330e@uwo.ca> <7350871cc135.55c03388@uwo.ca> <72a0e8ebe90d.55c033c6@uwo.ca> <7240f6e6df73.55c03404@uwo.ca> <72b0e73dac59.55c03442@uwo.ca> <73e099bbee6a.55c03480@uwo.ca> <7340aa8ad9cd.55c034be@uwo.ca> <7340daa0c093.55c034fc@uwo.ca> <73409960aec2.55c0353a@uwo.ca> <72a0a575c0b8.55c03578@uwo.ca> Message-ID: <72a089afa910.55bfef62@uwo.ca> Gloves and "Paraffin hazards?" 1. Flammable. White candles are made of paraffin wax. We light them ungloved. 2. Hot when melted, but not hot enough to cause even a first degree burn from a small amount on the skin. 3. Liquid enters clothing and solidifies therein. This is a real annoyance, but the safety experts couldn't care less. Wax is removable by soap & water at >60C (thank you, Ren? Buesa) or by dry-cleaning. 4. Solid wax has to be trimmed with a sharp knife to prepare for microtomy. Gloves thick enough to prevent a cut finger probably would impede this skilled task. 5. Every microtome has a dangerously sharp knife, less so in recent decades with short disposable blades. This is a hazard of microtomy, not of paraffin wax. It might be possible to cut, recover and mount sections while wearing very thin (surgical) rubber gloves, but why? John Kiernan London, Canada = = = On 03/08/15, "Johnson, Carole via Histonet" wrote: > This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? > > Carole Johnson > Carole Johnson, HT(ASCP)cm > New Mexico Department of Agriculture > Veterinary Diagnostic Services > 505.383.9299 > > To understand is to stand under, which is to look up, which is a good way to understand > > > > (http://lists.utsouthwestern.edu/mailman/listinfo/histonet) > > From David.Edmondson at christie.nhs.uk Tue Aug 4 06:47:30 2015 From: David.Edmondson at christie.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Tue, 4 Aug 2015 11:47:30 +0000 Subject: [Histonet] Marciafava-Bignami Disease I have re-logged into the system and maybe this will now communicate with Histologists out there, and hope that I do not get twice as many emails from Histonet# Message-ID: <20150804114731.CDD1A44935B@nhs-pd1e-esg101.ad1.nhs.net> ______________________________________________ From: Edmondson David (RBV) NHS Christie Tr Sent: 02 August 2015 16:40 To: 'histonet at lists.utsouthwestern.edu' Subject: Marciafava-Bignami Disease Hello out there, I have been working in Histology for forty years and am wondering at a cause for this disease. It was seen on MRI scans and they have told me to ease up on the alcohol, but I have never been remotely alcholic so I am giving up work in October and hope to live a little longer. Addition to work has been my only problem. They have tested a variety of bodily functions, touching my nose and foot movements together with assessing my ability to ride my motorcycle and make toast and tea, and then they let me off the acute neuro ward. My bike was chained up outside and making my way between lines of static traffic should have been a good sign. I am currently on massive doses of B vitamins but my GP reckons that they are of no use and I should just get better. So, is Xylene at the root of all my demyelination?? Regards David Edmondson This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. From CDavis at che-east.org Tue Aug 4 07:47:33 2015 From: CDavis at che-east.org (Davis, Cassie) Date: Tue, 4 Aug 2015 08:47:33 -0400 Subject: [Histonet] Cerner Message-ID: Good morning Histonet Folks, It seems my message yesterday was a little cryptic at best. We currently have SunQuest CoPath Plus with Meditech as our hospital wide system. We are changing to Cerner hospital wide and PathNet for our lab (because we were just merged a couple of years with another healthcare system & they want us to change) My main concern is if Cerner's PathNet plays well with Vantage. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From j.benavides at eae.csic.es Tue Aug 4 08:11:55 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Tue, 4 Aug 2015 15:11:55 +0200 Subject: [Histonet] Reagents changing in dewax/dehydration and tissue processor In-Reply-To: References: Message-ID: <55C0BA1B.3000000@eae.csic.es> Hi histonets, how often do you change alcohol/xylene for the dewax-dehydratation? we are running a manual system, with racks of 20 slides in 200 ml containers. These is the routine: *Dewax:* Xylene Xylene 100 ethanol 96 ethanol 60 ethanol *Dehydrate:* 96 ethanol 100 ethanol 100 ethanol Xylene Xylene trying to work out the cost per slides and for that we should established a fixed moment (number of slides?) when to change reagents. Similarly, what about the change of reagents in the tissue processor (talking about a cytadel 2000 here)? Thank a lot for you help!! All the best Julio From akemiat3377 at gmail.com Tue Aug 4 08:30:12 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Tue, 4 Aug 2015 06:30:12 -0700 Subject: [Histonet] Marciafava-Bignami Disease I have re-logged into the system and maybe this will now communicate with Histologists out there, and hope that I do not get twice as many emails from Histonet# In-Reply-To: <20150804114731.CDD1A44935B@nhs-pd1e-esg101.ad1.nhs.net> References: <20150804114731.CDD1A44935B@nhs-pd1e-esg101.ad1.nhs.net> Message-ID: GP?s are fine for general health issues, but I would certainly get more conclusive tests done by a neurologist, as well as contacting the best worker?s comp attorney in your area who has dealt with chemical exposure cases. Studies show we are #1 in this country for the most hazardous professions. It's safe compared to when I 1st started in this field in 1965! Well, I am a dinosaur from back in the day when we had inadequate ventilation, a shortage of fume hoods, inadequate education on hazards, safety and PPE's. In 1979 I started to work at OHSU in the surgical path lab, as well as doing research projects. We made up all of our own H&E's and special stains from scratch, as well as made up our own 10% NBF in 55 gallon drums without fume hoods, ventilation, masks or gloves. I also worked with Glyco-Methacrylate embedded tissues without hoods or gloves! Since it was a medical school, we did every special stain under the sun and dealt with about every chemical, reagent, acid and stain you could think of! We also smoked cigarettes and drank coffee in the lab while we embedded and cut! We sure were a naive group back then! In the early days, the facilities I worked in never had MSDS information available. In 1989, while at Emanuel Hospital, Portland, OR, I researched and compiled the MSDS information on all the chemicals, reagents, acids and stains that we used. That was the 1st eye opener to what me and my fellow histologists dealt with on a daily basis. In 1988 I had base line tests done because I was having issues with dizziness, balance, reflexes, and short term memory loss. In 1992, I had extensive neurological tests done, as well as a sural nerve bx taken from my right ankle. It was found that I had nerve damage, loss of balance, no reflexes, numbness in my fingers, hyper sensitivity and reduced feeling on my right side, hearing loss in my left ear, and an aedes pupil in my right eye. It was concluded these were the results from exposure to multiple toxic chemicals in an extremely small room with excessive heat and NO VENTILATION at current hospital I was working at. These health issues are irreversible. I just deal with it. I was the 1st person who won a case for this in the state of Oregon, but it had consequences. I won the battle, but lost the war! I now have Primary Biliary Cirrhosis (PBC). I was 1st diagnosed in 2008 for PBC. I think this condition was caused by my continual exposure to Multiple Toxic Chemicals. You may, or may not agree. A huge amount of the chemicals we deal with in the histology lab targets the liver and is absorbed through the skin or is inhaled. Here is the link for PBC. http://www.liverfoundation.org/abouttheliver/info/pbc/ Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com > On Aug 4, 2015, at 4:47 AM, Edmondson David (RBV) NHS Christie Tr via Histonet wrote: > > ______________________________________________ > From: Edmondson David (RBV) NHS Christie Tr > Sent: 02 August 2015 16:40 > To: 'histonet at lists.utsouthwestern.edu' > Subject: Marciafava-Bignami Disease > > Hello out there, > I have been working in Histology for forty years and am wondering at a cause for this disease. It was seen on MRI scans and they have told me to ease up on the alcohol, but I have never been remotely alcholic so I am giving up work in October and hope to live a little longer. Addition to work has been my only problem. > > They have tested a variety of bodily functions, touching my nose and foot movements together with assessing my ability to ride my motorcycle and make toast and tea, and then they let me off the acute neuro ward. > My bike was chained up outside and making my way between lines of static traffic should have been a good sign. > > I am currently on massive doses of B vitamins but my GP reckons that they are of no use and I should just get better. > > So, is Xylene at the root of all my demyelination?? > > Regards > > David Edmondson > > > > This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. > If you have received this e-mail in error you should not disseminate, distribute or copy it. > Please notify the sender immediately and delete this e-mail from your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone at leavittmgt.com Tue Aug 4 12:39:28 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Tue, 4 Aug 2015 17:39:28 +0000 Subject: [Histonet] FT NIGHT (evening shift) POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DC739@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann at leavittmgt.com if interested. *full time position Mon-Fri or Sun-Thurs 6PM-2:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of your shift) *MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Dana.Spencer at vidanthealth.com Tue Aug 4 13:29:23 2015 From: Dana.Spencer at vidanthealth.com (Spencer, Dana) Date: Tue, 4 Aug 2015 18:29:23 +0000 Subject: [Histonet] Significant/ Unexpected Findings Message-ID: <1438712963371.86857@vidanthealth.com> ANP.12175 Significant/Unexpected Findings How is everyone handling Significant or Unexpected Findings in Surgical Pathology? I would like to find out how other places handle these. Do your pathologists call them? Do you flag them for the clinician in an electronic medical record? Do you have a "Call Center" that handles them similiar to clinical lab "critical values"? Do you have a secretary contact the office and notify them you are faxing a report that needs to be flagged for the physician? I greatly appreciate your feedback! Dana Dana Spencer, CT(ASCP) Pathology Section Manager, Anatomic Pathology Vidant Medical Center 2100 Stantonsburg Road Greenville, NC 27835 (252) 847-5790 dana.spencer at vidanthealth.com ------------------------------------------------------------------------------ The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. ============================================================================== From HornHV at archildrens.org Tue Aug 4 14:04:27 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Tue, 4 Aug 2015 14:04:27 -0500 Subject: [Histonet] question for children's hospitals Message-ID: <25A4DE08332B19499904459F00AAACB71A39C4FB68@EVS1.archildrens.org> We are getting a large number of blocks and slides in our storage. Are the requirements for length of time the slides and blocks need to be kept any different for children, ages 0-21? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org From SteveM at mcclainlab.com Tue Aug 4 14:24:54 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 4 Aug 2015 19:24:54 +0000 Subject: [Histonet] Histonet Digest, Vol 141, Issue 4Surgical Pathologist Benchmark In-Reply-To: References: Message-ID: <033417FD-888C-43F5-A040-F4A39A312E95@mcclainlab.com> The best benchmark is the one you derive with your personnel and your pathologists in your system. The benchmark number you seek is not readily available because work is primarily dependent on the efficiency of your system of reporting, whether the pathologist has to spend time proofreading the gross descriptions, how organized the lab is at presenting cases, mechanisms for minimizing errors in identification, whether the pathologist is acquiring images and finally pathologist compensation and rewards. For an experienced pathologist, working in an efficient system, the upper limit could be as high as 500-700 specimens per shift. For less efficient systems and less experienced pathologists the upper limit may be as low as low as 50-100 specimens. Steve A. McClain, MD > On Aug 4, 2015, at 13:11, "histonet-request at lists.utsouthwestern.edu" wrote: > > On Monday, August 3, 2015 5:20 PM, "Vickroy, James via Histonet" wrote: > > > I realize this is a question that may be impossible to answer given all of the variables in pathology labs, specimens, physicians, etc.? However here goes:? For those that work in a lab that primarily does biopsies ( mainly gi biopsies) what is the average number of slides that a pathologist reads each day.? ? I have looked online for parameters recommended however I haven't had a whole lot of luck.? There are plenty of figures on numbers of blocks and slides for histotechs but just wondered if anyone was using any either national benchmarks or local practices. From hist10 at aol.com Tue Aug 4 14:27:48 2015 From: hist10 at aol.com (Roslyn Macon) Date: Tue, 4 Aug 2015 15:27:48 -0400 Subject: [Histonet] Handline paraffin In-Reply-To: References: Message-ID: <557BED57-1865-440C-9931-32B45FC7D94F@aol.com> Sent from my iPhone > On Aug 3, 2015, at 10:03 PM, Karen Stephanson via Histonet wrote: > > Is there any written documentation? > On Aug 3, 2015 11:10 AM, Rene J Buesa via Histonet wrote: > It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or "irritating" substance. It is just an oil of high molecular weight (mineral oil)Ren? > > > On Monday, August 3, 2015 12:48 PM, "Johnson, Carole via Histonet" wrote: > > > This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? > > Carole Johnson > Carole Johnson, HT(ASCP)cm > New Mexico Department of Agriculture > Veterinary Diagnostic Services > 505.383.9299 > > To understand is to stand under, which is to look up, which is a good way to understand > > > > > Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer at mdanderson.org Tue Aug 4 14:49:41 2015 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Tue, 4 Aug 2015 19:49:41 +0000 Subject: [Histonet] Marciafava-Bignami Disease Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88224FE68D@D1PWPEXMBX05.mdanderson.edu> This is very interesting. I have worked in the field for over 20 yrs, and was pregnant while working in a small lab. Mostly everything was manual, and I did not use all of the safety precautions I should have (my fault). My son had severe speech and language delay as well as a language processing issue when he was smaller, and now stutters. I have often wondered (as most parents would) if my lack of precautions may have contributed. We stress the safety issues to the students daily, to get it all in their heads. I often tell people that I am so happy because I work with xylene, and I feel real good. So far, I don't see any noticeable symptoms of anything wrong, just the usual-decreased olfactory sensing, repetitive motion issues, plantar fasciitis, and varicose veins. Hair loss was an issue at one time, but that was attributed to PCOS. Now that I am not in the lab full time anymore it has decreased since I went natural (I'm African-american). One thing I have noticed is my C-reactive protein is high, and I take a statin for that. I have no liver issues, and my functions appear to be normal. Next time I go in for a physical, I will have my workplace hazards documented in my EMR. I will look up Primary Biliary Cirrhosis (PBC), to get some more information, especially since I was born jaundiced and I am anemic. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 11 Date: Tue, 4 Aug 2015 06:30:12 -0700 From: Eileen Akemi Allison To: "Edmondson David (RBV) NHS Christie Tr" Cc: Histonet Subject: Re: [Histonet] Marciafava-Bignami Disease I have re-logged into the system and maybe this will now communicate with Histologists out there, and hope that I do not get twice as many emails from Histonet# Message-ID: Content-Type: text/plain; charset=utf-8 GP?s are fine for general health issues, but I would certainly get more conclusive tests done by a neurologist, as well as contacting the best worker?s comp attorney in your area who has dealt with chemical exposure cases. Studies show we are #1 in this country for the most hazardous professions. It's safe compared to when I 1st started in this field in 1965! Well, I am a dinosaur from back in the day when we had inadequate ventilation, a shortage of fume hoods, inadequate education on hazards, safety and PPE's. In 1979 I started to work at OHSU in the surgical path lab, as well as doing research projects. We made up all of our own H&E's and special stains from scratch, as well as made up our own 10% NBF in 55 gallon drums without fume hoods, ventilation, masks or gloves. I also worked with Glyco-Methacrylate embedded tissues without hoods or gloves! Since it was a medical school, we did every special stain under the sun and dealt with about every chemical, reagent, acid and stain you could think of! We also smoked cigarettes and drank coffee in the lab while we embedded and cut! We sure were a naive group back then! In the early days, the facilities I worked in never had MSDS information available. In 1989, while at Emanuel Hospital, Portland, OR, I researched and compiled the MSDS information on all the chemicals, reagents, acids and stains that we used. That was the 1st eye opener to what me and my fellow histologists dealt with on a daily basis. In 1988 I had base line tests done because I was having issues with dizziness, balance, reflexes, and short term memory loss. In 1992, I had extensive neurological tests done, as well as a sural nerve bx taken from my right ankle. It was found that I had nerve damage, loss of balance, no reflexes, numbness in my fingers, hyper sensitivity and reduced feeling on my right side, hearing loss in my left ear, and an aedes pupil in my right eye. It was concluded these were the results from exposure to multiple toxic chemicals in an extremely small room with excessive heat and NO VENTILATION at current hospital I was working at. These health issues are irreversible. I just deal with it. I was the 1st person who won a case for this in the state of Oregon, but it had consequences. I won the battle, but lost the war! I now have Primary Biliary Cirrhosis (PBC). I was 1st diagnosed in 2008 for PBC. I think this condition was caused by my continual exposure to Multiple Toxic Chemicals. You may, or may not agree. A huge amount of the chemicals we deal with in the histology lab targets the liver and is absorbed through the skin or is inhaled. Here is the link for PBC. http://www.liverfoundation.org/abouttheliver/info/pbc/ Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com > On Aug 4, 2015, at 4:47 AM, Edmondson David (RBV) NHS Christie Tr via Histonet wrote: > > ______________________________________________ > From: Edmondson David (RBV) NHS Christie Tr > Sent: 02 August 2015 16:40 > To: 'histonet at lists.utsouthwestern.edu' > Subject: Marciafava-Bignami Disease > > Hello out there, > I have been working in Histology for forty years and am wondering at a cause for this disease. It was seen on MRI scans and they have told me to ease up on the alcohol, but I have never been remotely alcholic so I am giving up work in October and hope to live a little longer. Addition to work has been my only problem. > > They have tested a variety of bodily functions, touching my nose and foot movements together with assessing my ability to ride my motorcycle and make toast and tea, and then they let me off the acute neuro ward. > My bike was chained up outside and making my way between lines of static traffic should have been a good sign. > > I am currently on massive doses of B vitamins but my GP reckons that they are of no use and I should just get better. > > So, is Xylene at the root of all my demyelination?? > > Regards > > David Edmondson > > > > This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. > If you have received this e-mail in error you should not disseminate, distribute or copy it. > Please notify the sender immediately and delete this e-mail from your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 141, Issue 4 **************************************** From CIngles at uwhealth.org Tue Aug 4 16:11:42 2015 From: CIngles at uwhealth.org (Ingles Claire) Date: Tue, 4 Aug 2015 21:11:42 +0000 Subject: [Histonet] Marciafava-Bignami Disease In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC88224FE68D@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC88224FE68D@D1PWPEXMBX05.mdanderson.edu> Message-ID: I know I watch my exposure to Formalin and Xylene especially. I was diagnosed with NASH in 2007 (who knows how long before then, since I am asymptomatic) when I had a Pituitary Adenoma that had hemorraged (Boy talk about a headache!) Anyway, I have always wondered about the cause. I thought it was genetic as my father also had it for over 40 years before finally having a transplant. But I wonder if it was his exposure to jet fuel, etc. as he was a jet mechanic for 20 years in the USAF plus another 15-20 with a private company. So far my liver has been stable even getting a bit better to the point of being just a hair above the normal range. I do everything under the sun in my lab. Mohs, Grossing, plus all the routine stuff of cutting and staining, etc. I have been able so far to keep the repetitive injuries at bay, and Dansco shoes have saved my feet from the Plantar Faciitis (had that about a year straight at one point.). We had managed to keep xylene out of our lab for years by using Propar, but one of the Pathologists insisted on having a tape coverslipper. We still use it as little as possible. I had a co-worker, when she was pregnant wasn't allowed to gross (hence contact with formalin). Because of the NASH I get blood tests done every 6 months. It will be interesting to see if they have changed possibly due to the exposure to xylene, which I haven't had exposure to for years otherwise. Claire ________________________________________ From: Mayer,Toysha N via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 04, 2015 2:49 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Marciafava-Bignami Disease This is very interesting. I have worked in the field for over 20 yrs, and was pregnant while working in a small lab. Mostly everything was manual, and I did not use all of the safety precautions I should have (my fault). My son had severe speech and language delay as well as a language processing issue when he was smaller, and now stutters. I have often wondered (as most parents would) if my lack of precautions may have contributed. We stress the safety issues to the students daily, to get it all in their heads. I often tell people that I am so happy because I work with xylene, and I feel real good. So far, I don't see any noticeable symptoms of anything wrong, just the usual-decreased olfactory sensing, repetitive motion issues, plantar fasciitis, and varicose veins. Hair loss was an issue at one time, but that was attributed to PCOS. Now that I am not in the lab full time anymore it has decreased since I went natural (I'm African-american). One thing I have noticed is my C-reactive protein is high, and I take a statin for that. I have no liver issues, and my functions appear to be normal. Next time I go in for a physical, I will have my workplace hazards documented in my EMR. I will look up Primary Biliary Cirrhosis (PBC), to get some more information, especially since I was born jaundiced and I am anemic. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 11 Date: Tue, 4 Aug 2015 06:30:12 -0700 From: Eileen Akemi Allison To: "Edmondson David (RBV) NHS Christie Tr" Cc: Histonet Subject: Re: [Histonet] Marciafava-Bignami Disease I have re-logged into the system and maybe this will now communicate with Histologists out there, and hope that I do not get twice as many emails from Histonet# Message-ID: Content-Type: text/plain; charset=utf-8 GP?s are fine for general health issues, but I would certainly get more conclusive tests done by a neurologist, as well as contacting the best worker?s comp attorney in your area who has dealt with chemical exposure cases. Studies show we are #1 in this country for the most hazardous professions. It's safe compared to when I 1st started in this field in 1965! Well, I am a dinosaur from back in the day when we had inadequate ventilation, a shortage of fume hoods, inadequate education on hazards, safety and PPE's. In 1979 I started to work at OHSU in the surgical path lab, as well as doing research projects. We made up all of our own H&E's and special stains from scratch, as well as made up our own 10% NBF in 55 gallon drums without fume hoods, ventilation, masks or gloves. I also worked with Glyco-Methacrylate embedded tissues without hoods or gloves! Since it was a medical school, we did every special stain under the sun and dealt with about every chemical, reagent, acid and stain you could think of! We also smoked cigarettes and drank coffee in the lab while we embedded and cut! We sure were a naive group back then! In the early days, the facilities I worked in never had MSDS information available. In 1989, while at Emanuel Hospital, Portland, OR, I researched and compiled the MSDS information on all the chemicals, reagents, acids and stains that we used. That was the 1st eye opener to what me and my fellow histologists dealt with on a daily basis. In 1988 I had base line tests done because I was having issues with dizziness, balance, reflexes, and short term memory loss. In 1992, I had extensive neurological tests done, as well as a sural nerve bx taken from my right ankle. It was found that I had nerve damage, loss of balance, no reflexes, numbness in my fingers, hyper sensitivity and reduced feeling on my right side, hearing loss in my left ear, and an aedes pupil in my right eye. It was concluded these were the results from exposure to multiple toxic chemicals in an extremely small room with excessive heat and NO VENTILATION at current hospital I was working at. These health issues are irreversible. I just deal with it. I was the 1st person who won a case for this in the state of Oregon, but it had consequences. I won the battle, but lost the war! I now have Primary Biliary Cirrhosis (PBC). I was 1st diagnosed in 2008 for PBC. I think this condition was caused by my continual exposure to Multiple Toxic Chemicals. You may, or may not agree. A huge amount of the chemicals we deal with in the histology lab targets the liver and is absorbed through the skin or is inhaled. Here is the link for PBC. http://www.liverfoundation.org/abouttheliver/info/pbc/ Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com > On Aug 4, 2015, at 4:47 AM, Edmondson David (RBV) NHS Christie Tr via Histonet wrote: > > ______________________________________________ > From: Edmondson David (RBV) NHS Christie Tr > Sent: 02 August 2015 16:40 > To: 'histonet at lists.utsouthwestern.edu' > Subject: Marciafava-Bignami Disease > > Hello out there, > I have been working in Histology for forty years and am wondering at a cause for this disease. It was seen on MRI scans and they have told me to ease up on the alcohol, but I have never been remotely alcholic so I am giving up work in October and hope to live a little longer. Addition to work has been my only problem. > > They have tested a variety of bodily functions, touching my nose and foot movements together with assessing my ability to ride my motorcycle and make toast and tea, and then they let me off the acute neuro ward. > My bike was chained up outside and making my way between lines of static traffic should have been a good sign. > > I am currently on massive doses of B vitamins but my GP reckons that they are of no use and I should just get better. > > So, is Xylene at the root of all my demyelination?? > > Regards > > David Edmondson > > > > This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. > If you have received this e-mail in error you should not disseminate, distribute or copy it. > Please notify the sender immediately and delete this e-mail from your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 141, Issue 4 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Tue Aug 4 17:07:26 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 4 Aug 2015 22:07:26 +0000 (UTC) Subject: [Histonet] Handline paraffin In-Reply-To: <557BED57-1865-440C-9931-32B45FC7D94F@aol.com> References: <557BED57-1865-440C-9931-32B45FC7D94F@aol.com> Message-ID: <2125105960.737504.1438726046312.JavaMail.yahoo@mail.yahoo.com> Use the paraffin MSDS Ren? On Tuesday, August 4, 2015 3:27 PM, Roslyn Macon wrote: Sent from my iPhone > On Aug 3, 2015, at 10:03 PM, Karen Stephanson via Histonet wrote: > > Is there any written documentation? > On Aug 3, 2015 11:10 AM, Rene J Buesa via Histonet wrote: > It is absolutely NOT necessary to wear gloves when working with paraffin. This is NOT a harmful or "irritating" substance. It is just an oil of high molecular weight (mineral oil)Ren? > > >? ? On Monday, August 3, 2015 12:48 PM, "Johnson, Carole via Histonet" wrote: > > > This is kind of an odd question, but I was asked by a pathologist for any SOPs or references for the necessity of wearing gloves when embedding and working with paraffin. I am not aware of sources other than the MSDS for the different formulations. Does anyone require gloves to be worn during embedding, specifically related to paraffin hazards? > > Carole Johnson > Carole Johnson, HT(ASCP)cm > New Mexico Department of Agriculture > Veterinary Diagnostic Services > 505.383.9299 > > To understand is to stand under, which is to look up, which is a good way to understand > > > > > Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From riepopp2 at gmail.com Tue Aug 4 17:09:52 2015 From: riepopp2 at gmail.com (Laurie Finch) Date: Tue, 4 Aug 2015 17:09:52 -0500 Subject: [Histonet] Glutamine synthetase staining Message-ID: Hi Linda, What about Cleveland clinic? Regards, Laurie From akemiat3377 at gmail.com Tue Aug 4 23:29:13 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Tue, 4 Aug 2015 21:29:13 -0700 Subject: [Histonet] Marciafava-Bignami Disease In-Reply-To: References: <47E9B2C01DDDD94881EACD2DC44EBC88224FE68D@D1PWPEXMBX05.mdanderson.edu> Message-ID: <1EEB47FB-C92E-46A1-A27C-05F2E95C4F81@gmail.com> From my archives regarding my dear friend (Chuck) Charles Churukian: > December 26, 2010 ? > I just heard from Irene Churukian today. Please put Chuck in your prayers. Irene said this: CLL cells changed in character so Chuck began chemo treatments on Dec. 1. His second one was Dec. 21... He has six altogether -The first cycle was difficult... he was very weak-in bed most of the time. He is now improving-thankfully. We are praying that by April, he will be in remission after all the treatments are completed. RIP Chuck Xo Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com > On Aug 4, 2015, at 2:11 PM, Ingles Claire via Histonet wrote: > > I know I watch my exposure to Formalin and Xylene especially. I was diagnosed with NASH in 2007 (who knows how long before then, since I am asymptomatic) when I had a Pituitary Adenoma that had hemorraged (Boy talk about a headache!) Anyway, I have always wondered about the cause. I thought it was genetic as my father also had it for over 40 years before finally having a transplant. But I wonder if it was his exposure to jet fuel, etc. as he was a jet mechanic for 20 years in the USAF plus another 15-20 with a private company. So far my liver has been stable even getting a bit better to the point of being just a hair above the normal range. I do everything under the sun in my lab. Mohs, Grossing, plus all the routine stuff of cutting and staining, etc. I have been able so far to keep the repetitive injuries at bay, and Dansco shoes have saved my feet from the Plantar Faciitis (had that about a year straight at one point.). We had managed to keep xylene out of our lab for years by using Propar, but one of the Pathologists insisted on having a tape coverslipper. We still use it as little as possible. I had a co-worker, when she was pregnant wasn't allowed to gross (hence contact with formalin). Because of the NASH I get blood tests done every 6 months. It will be interesting to see if they have changed possibly due to the exposure to xylene, which I haven't had exposure to for years otherwise. > Claire > > ________________________________________ > From: Mayer,Toysha N via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Tuesday, August 04, 2015 2:49 PM > To: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] Marciafava-Bignami Disease > > This is very interesting. I have worked in the field for over 20 yrs, and was pregnant while working in a small lab. Mostly everything was manual, and I did not use all of the safety precautions I should have (my fault). My son had severe speech and language delay as well as a language processing issue when he was smaller, and now stutters. I have often wondered (as most parents would) if my lack of precautions may have contributed. We stress the safety issues to the students daily, to get it all in their heads. I often tell people that I am so happy because I work with xylene, and I feel real good. So far, I don't see any noticeable symptoms of anything wrong, just the usual-decreased olfactory sensing, repetitive motion issues, plantar fasciitis, and varicose veins. Hair loss was an issue at one time, but that was attributed to PCOS. Now that I am not in the lab full time anymore it has decreased since I went natural (I'm African-american). One thing I have noticed is my C-reactive protein is high, and I take a statin for that. I have no liver issues, and my functions appear to be normal. Next time I go in for a physical, I will have my workplace hazards documented in my EMR. > I will look up Primary Biliary Cirrhosis (PBC), to get some more information, especially since I was born jaundiced and I am anemic. > > > Sincerely, > > Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) > Instructor/Education Coordinator > Program in Histotechnology > School of Health Professions > UT M.D. Anderson Cancer Center > 713.563-3481 > > > Message: 11 > Date: Tue, 4 Aug 2015 06:30:12 -0700 > From: Eileen Akemi Allison > To: "Edmondson David (RBV) NHS Christie Tr" > > Cc: Histonet > Subject: Re: [Histonet] Marciafava-Bignami Disease I have re-logged > into the system and maybe this will now communicate with Histologists > out there, and hope that I do not get twice as many emails from > Histonet# > Message-ID: > Content-Type: text/plain; charset=utf-8 > > GP?s are fine for general health issues, but I would certainly get more conclusive tests done by a neurologist, as well as contacting the best worker?s comp attorney in your area who has dealt with chemical exposure cases. > > Studies show we are #1 in this country for the most hazardous professions. It's safe compared to when I 1st started in this field in 1965! Well, I am a dinosaur from back in the day when we had inadequate ventilation, a shortage of fume hoods, inadequate education on hazards, safety and PPE's. > > In 1979 I started to work at OHSU in the surgical path lab, as well as doing research projects. We made up all of our own H&E's and special stains from scratch, as well as made up our own 10% NBF in 55 gallon drums without fume hoods, ventilation, masks or gloves. I also worked with Glyco-Methacrylate embedded tissues without hoods or gloves! Since it was a medical school, we did every special stain under the sun and dealt with about every chemical, reagent, acid and stain you could think of! We also smoked cigarettes and drank coffee in the lab while we embedded and cut! We sure were a naive group back then! > > In the early days, the facilities I worked in never had MSDS information available. In 1989, while at Emanuel Hospital, Portland, OR, I researched and compiled the MSDS information on all the chemicals, reagents, acids and stains that we used. That was the 1st eye opener to what me and my fellow histologists dealt with on a daily basis. > > In 1988 I had base line tests done because I was having issues with dizziness, balance, reflexes, and short term memory loss. In 1992, I had extensive neurological tests done, as well as a sural nerve bx taken from my right ankle. It was found that I had nerve damage, loss of balance, no reflexes, numbness in my fingers, hyper sensitivity and reduced feeling on my right side, hearing loss in my left ear, and an aedes pupil in my right eye. It was concluded these were the results from exposure to multiple toxic chemicals in an extremely small room with excessive heat and NO VENTILATION at current hospital I was working at. These health issues are irreversible. I just deal with it. I was the 1st person who won a case for this in the state of Oregon, but it had consequences. I won the battle, but lost the war! > > I now have Primary Biliary Cirrhosis (PBC). I was 1st diagnosed in 2008 for PBC. I think this condition was caused by my continual exposure to Multiple Toxic Chemicals. You may, or may not agree. A huge amount of the chemicals we deal with in the histology lab targets the liver and is absorbed through the skin or is inhaled. Here is the link for PBC. http://www.liverfoundation.org/abouttheliver/info/pbc/ > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > > >> On Aug 4, 2015, at 4:47 AM, Edmondson David (RBV) NHS Christie Tr via Histonet wrote: >> >> ______________________________________________ >> From: Edmondson David (RBV) NHS Christie Tr >> Sent: 02 August 2015 16:40 >> To: 'histonet at lists.utsouthwestern.edu' >> Subject: Marciafava-Bignami Disease >> >> Hello out there, >> I have been working in Histology for forty years and am wondering at a cause for this disease. It was seen on MRI scans and they have told me to ease up on the alcohol, but I have never been remotely alcholic so I am giving up work in October and hope to live a little longer. Addition to work has been my only problem. >> >> They have tested a variety of bodily functions, touching my nose and foot movements together with assessing my ability to ride my motorcycle and make toast and tea, and then they let me off the acute neuro ward. >> My bike was chained up outside and making my way between lines of static traffic should have been a good sign. >> >> I am currently on massive doses of B vitamins but my GP reckons that they are of no use and I should just get better. >> >> So, is Xylene at the root of all my demyelination?? >> >> Regards >> >> David Edmondson >> >> >> >> This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. >> If you have received this e-mail in error you should not disseminate, distribute or copy it. >> Please notify the sender immediately and delete this e-mail from your system. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 141, Issue 4 > **************************************** > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis at seattlechildrens.org Wed Aug 5 13:04:23 2015 From: patrick.lewis at seattlechildrens.org (Lewis, Patrick) Date: Wed, 5 Aug 2015 18:04:23 +0000 Subject: [Histonet] Can Ice Crystal artifact pick up AEC substrate. Message-ID: <3903BE18914F4440834F0E620415FFCA3CB924DA@PPWEXD01d.childrens.sea.kids> Hi Everyone, I am seeing some red granular staining with my AEC substrate, and I am not sure if its real, or Ice crystal artifact, or debris from the slide directly. It's in several areas and is consistent between duplicates. So can I rule out slide debris? How deep would Ice crystals go in a block. If I cut 10-15 7 uM sections deeper would I eliminate ice crystals from one slide to another? I don't have any pictures yet, but I can try and post it when I do. Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From SteveM at mcclainlab.com Wed Aug 5 13:11:39 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 5 Aug 2015 18:11:39 +0000 Subject: [Histonet] Histonet Digest, Vol 141, Issue 5 unexpected AP findings In-Reply-To: References: Message-ID: We have pathologists call nearly all unexpected findings. Experience has shown that some findings we initially viewed as "insignificant" later proved to be problematic. We document calls to and from physicians (slide reviews also) in the reporting database record and usually in the report with an addendum report where treatment options are discussed. Pre-emptive communication is preferable to cases falling through the cracks. For example, yesterday I called the surgeon about a toe amputation with fungal osteomyelitis, not because it needed treatment, because it probably does not require systemic antifungals. Significant diseases ( melanoma, lymphoma, leukemia, metastasis, Merkel cell carcinoma, sarcoma) to surgeons whom I do not know are also called. A) it may be useful to steer/ refer the patient toward surgeon where appropriate; B) it is useful to communicate "lethal diagnoses" redundantly even in the age of electronic records. C) additional clinical data can be obtained when needed. D) direct communication shortens the time until action/treatment is scheduled. When the patient calls requesting their own report or wants to discuss w pathologist, the surgeons are alerted, more as a courtesy than a requirement. Those calls are documented in an addendum report. These are common examples, I am sure there are others. Steve A. McClain, MD > On Aug 5, 2015, at 13:26, "histonet-request at lists.utsouthwestern.edu" wrote: > > ANP.12175 Significant/Unexpected Findings > > > > How is everyone handling Significant or Unexpected Findings in Surgical Pathology? I would like to find out how other places handle these. > > Do your pathologists call them? > > Do you flag them for the clinician in an electronic medical record? > > Do you have a "Call Center" that handles them similiar to clinical lab "critical values"? > > Do you have a secretary contact the office and notify them you are faxing a report that needs to be flagged for the physician? > > > > I greatly appreciate your feedback! > > > > Dana > > Dana Spencer, CT(ASCP) > Pathology Section Manager, Anatomic Pathology > Vidant Medical Center > 2100 Stantonsburg Road > Greenville, NC 27835 > (252) 847-5790 > dana.spencer at vidanthealth.com From tbraud at holyredeemer.com Wed Aug 5 13:20:11 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 5 Aug 2015 18:20:11 +0000 Subject: [Histonet] Histonet Digest, Vol 141, Issue 5 In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F8024C87@HRHEX02-HOS.holyredeemer.local> For us, unexpected findings are noted in the report as such. The pathologist calls and personally notifies the surgeon. The date/time and person called is noted in the report. Example: NOTE: Unexpected findings of XXXXXXXX discussed with Dr XXXX on 7/31/2015, 1115. Hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 From NLeon at chw.org Wed Aug 5 13:51:02 2015 From: NLeon at chw.org (Leon, Nicole) Date: Wed, 5 Aug 2015 18:51:02 +0000 Subject: [Histonet] Block and slide cabinets Message-ID: <9F8F31F30681F745905CBC817A486050198DC9@C1XCHPWS13.chwi.chswi.org> I'm wondering if anyone is aware of any companies that purchase used block and slide cabinets. In the coming months I will have many of both types of cabinets due to a block/slide storage reorganization. Any feedback is appreciated. Thanks! Nicole Anne Leon BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin This message originates from Children's Hospital of Wisconsin, and may contain information that is privileged and confidential. It is intended only for the use of the designated recipient. Any review, dissemination, distribution, or copying of this message by anyone other than the intended recipient is prohibited. All personal messages express views solely of the sender, are not to be attributed to Children's Hospital of Wisconsin, and may not be copied or distributed without this disclaimer. If you have received this message in error, please notify the sender and destroy the original message. Thank you. From bcooper at chla.usc.edu Wed Aug 5 13:56:55 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 5 Aug 2015 18:56:55 +0000 Subject: [Histonet] Spirochete Control Block? Message-ID: Good afternoon Histonetters! I'm fairly certain my chances of seeing a puppy dog, riding on the back of a unicorn, sitting under a blue moon are greater, but does anyone have a block of spirochetes they'd be willing to share? Recently, we've gotten some great controls from these posts and thought we'd give this a shot! We're more than willing to trade! Please contact me directly if you don't want to post your responses. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From JRobinson at pathology-associates.com Wed Aug 5 14:25:40 2015 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Wed, 5 Aug 2015 19:25:40 +0000 Subject: [Histonet] Spirochete Control Block? In-Reply-To: References: Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D9ED8A@PAEXCH1.PathologyAssociates.local> Hi Brian- we just buy controls for spirochetes. I did procure a control block many years ago from someone working with pigs in a vet histology facility in Missouri. It was quite good as I recall. That may be an option for you. We run so few Steiners that it is not worthwhile for me to try and find a control block. Good Luck! Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Cooper, Brian via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, August 05, 2015 11:57 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Spirochete Control Block? Good afternoon Histonetters! I'm fairly certain my chances of seeing a puppy dog, riding on the back of a unicorn, sitting under a blue moon are greater, but does anyone have a block of spirochetes they'd be willing to share? Recently, we've gotten some great controls from these posts and thought we'd give this a shot! We're more than willing to trade! Please contact me directly if you don't want to post your responses. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From David.Edmondson at christie.nhs.uk Thu Aug 6 08:54:38 2015 From: David.Edmondson at christie.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Thu, 6 Aug 2015 13:54:38 +0000 Subject: [Histonet] Marchiafava-Bignami and Xylene Message-ID: <20150806135439.C76514487DF@nhs-pd1e-esg103.ad1.nhs.net> So I have had the whatever to spell it right, bother it This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. From nicole at dlcjax.com Thu Aug 6 11:04:36 2015 From: nicole at dlcjax.com (Nicole Tatum) Date: Thu, 6 Aug 2015 16:04:36 +0000 Subject: [Histonet] Clia requirements on specimens collected from satelite offices. Message-ID: Histos, I have a question. We are a Derm POL and we recently opened a satellite office. I have been putting the satellite address on the pathology requisitions but do the path reports need to have that address or the address of the main lab where they were processed. Bacisly does CLIA want the address where the specimen was from, or the address where it was processed. Thank You, Nicole Tatum From Natacha.Mitchell at harrishealth.org Thu Aug 6 11:08:40 2015 From: Natacha.Mitchell at harrishealth.org (Mitchell, Natacha A) Date: Thu, 6 Aug 2015 16:08:40 +0000 Subject: [Histonet] Embedding Centers Message-ID: <6441A38AD60C3543B5861E7E678D0ABFB2CFED@hhmsg02.hchd.local> Hello fellow histonetters, Can you all provide input on experience with the Leica EG 1150 embedding center, Thermo HistoStar and Tissue-Tek TEC 5. I have worked with all but the Leica model. Thanks in advance!! Natacha Mitchell CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From MWhite at mhs.net Thu Aug 6 12:01:25 2015 From: MWhite at mhs.net (White, Marcia) Date: Thu, 6 Aug 2015 17:01:25 +0000 Subject: [Histonet] Clia requirements on specimens collected from satelite offices. In-Reply-To: References: Message-ID: <8B4B5BF3FCB6D24CBEAA63ACA5FDAD3B514931AE@MHSEXMB04.mhs.net> Where it was processed and diagnosis rendered Marcia M White Pathology Manager Memorial Regional Hospital 954-265-5371-office 954-967-7627-fax MWhite at mhs.net -----Original Message----- From: Nicole Tatum [mailto:nicole at dlcjax.com] Sent: Thursday, August 06, 2015 12:05 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Clia requirements on specimens collected from satelite offices. Histos, I have a question. We are a Derm POL and we recently opened a satellite office. I have been putting the satellite address on the pathology requisitions but do the path reports need to have that address or the address of the main lab where they were processed. Bacisly does CLIA want the address where the specimen was from, or the address where it was processed. Thank You, Nicole Tatum CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From jclark at pcnm.com Thu Aug 6 15:27:48 2015 From: jclark at pcnm.com (Joanne Clark) Date: Thu, 6 Aug 2015 20:27:48 +0000 Subject: [Histonet] Manual Cassette Labelling Message-ID: <0494A7D4E8CC254EA2FB81464982E378D9ABD883@S10MAILD001N3.SH10.lan> Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From CIngles at uwhealth.org Thu Aug 6 15:50:05 2015 From: CIngles at uwhealth.org (Ingles Claire) Date: Thu, 6 Aug 2015 20:50:05 +0000 Subject: [Histonet] Manual Cassette Labelling In-Reply-To: <0494A7D4E8CC254EA2FB81464982E378D9ABD883@S10MAILD001N3.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E378D9ABD883@S10MAILD001N3.SH10.lan> Message-ID: We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to. Claire ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 3:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Manual Cassette Labelling Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkienitz at orclinic.com Thu Aug 6 15:50:58 2015 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Thu, 6 Aug 2015 13:50:58 -0700 Subject: [Histonet] Manual Cassette Labelling In-Reply-To: <0494A7D4E8CC254EA2FB81464982E378D9ABD883@S10MAILD001N3.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E378D9ABD883@S10MAILD001N3.SH10.lan> Message-ID: <41400FFE517878449D89114DD2526090196602D20F@tocmail1.tocad.orclinic.com> Epic Scientific Super Moist Markers by Creative Waste Solutions 503-657-5711 They will not let you down :) Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 1:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Manual Cassette Labelling Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Natacha.Mitchell at harrishealth.org Thu Aug 6 16:03:06 2015 From: Natacha.Mitchell at harrishealth.org (Mitchell, Natacha A) Date: Thu, 6 Aug 2015 21:03:06 +0000 Subject: [Histonet] Embedding Centers Message-ID: <6441A38AD60C3543B5861E7E678D0ABFB2D5DA@hhmsg02.hchd.local> My fellow histonetters, Thank you all so much for the prompt response. All the posts/emails were very helpful and I must say this site is a priceless source of knowledge, wisdom and resource!!! Natacha Mitchell CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From DKBoyd at chs.net Fri Aug 7 06:35:19 2015 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Fri, 7 Aug 2015 11:35:19 +0000 Subject: [Histonet] Manual Cassette Labelling In-Reply-To: References: <0494A7D4E8CC254EA2FB81464982E378D9ABD883@S10MAILD001N3.SH10.lan>, Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F3F858@TN001WEXMBX014.US.chs.net> We use KP Marker Plus from Mercedes Medical, www.mercedesmedical.com or 1-800-331-2716. Alcohol and Americlear resistant. We do not use Xylene. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Ingles Claire via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 4:50 PM To: Joanne Clark; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Manual Cassette Labelling We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to. Claire ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 3:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Manual Cassette Labelling Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From LBUSTAMANTE at cvm.tamu.edu Fri Aug 7 09:49:38 2015 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Fri, 7 Aug 2015 14:49:38 +0000 Subject: [Histonet] FW: Hyde-Away In-Reply-To: <585485517.784638.1438957709794.JavaMail.yahoo@mail.yahoo.com> References: <585485517.784638.1438957709794.JavaMail.yahoo@mail.yahoo.com> Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC901560C1ECF@CVMMB02.cvm.tamu.edu> Can someone help her please? Thank you very much. Lin Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From: Coral Gonzalez [mailto:rayengonzalez at yahoo.com] Sent: Friday, August 07, 2015 9:28 AM To: Bustamante, Lin Subject: Hyde-Away Hello, Please when you can, ask on Histonet about the ppm legal limit for draining formalin neutralized with Hyde Away. Thank you, Rayen Gonzalez B.S., HT (ASCP) From badams at acadianagastro.com Fri Aug 7 12:29:22 2015 From: badams at acadianagastro.com (Brent Adams) Date: Fri, 7 Aug 2015 17:29:22 +0000 Subject: [Histonet] Histonet Digest, Vol 141, Issue 7 topic 2 In-Reply-To: References: Message-ID: I had the same problem with StatLab markers. I would have issues when I let tissue set in formalin bottles all night. I would skip the formalin steps on the processor and go straight to 70% alcohol. This would almost completely erase the writing on the cassettes. The product manager informed me The Statmark Pens are made with an aqueous ink and if exposed to fingers prints or other solutions prior to being set by submerged in 100% Alcohol they will be erased or badly smudged. I keep a container of 100% alcohol on my grossing bench and dip the cassettes first before adding tissue from the containers. by time I add the tissue and close the cassette lid the alcohol has evaporated and locks the ink into the plastic cassette. If they are still slightly wet or you have alcohol on your hands they will still smudge so be careful handling cassettes. I have not had issues since going to this process. Brent Adams ? HT, BS, LPN, www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, August 7, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 141, Issue 7 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Clia requirements on specimens collected from satelite offices. (White, Marcia) 2. Manual Cassette Labelling (Joanne Clark) 3. Re: Manual Cassette Labelling (Ingles Claire) 4. Re: Manual Cassette Labelling (Kienitz, Kari) 5. Embedding Centers (Mitchell, Natacha A) 6. Re: Manual Cassette Labelling (Boyd, Debbie M) 7. FW: Hyde-Away (Bustamante, Lin) ---------------------------------------------------------------------- Message: 1 Date: Thu, 6 Aug 2015 17:01:25 +0000 From: "White, Marcia" To: "'Nicole Tatum'" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Clia requirements on specimens collected from satelite offices. Message-ID: <8B4B5BF3FCB6D24CBEAA63ACA5FDAD3B514931AE at MHSEXMB04.mhs.net> Content-Type: text/plain; charset="us-ascii" Where it was processed and diagnosis rendered Marcia M White Pathology Manager Memorial Regional Hospital 954-265-5371-office 954-967-7627-fax MWhite at mhs.net -----Original Message----- From: Nicole Tatum [mailto:nicole at dlcjax.com] Sent: Thursday, August 06, 2015 12:05 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Clia requirements on specimens collected from satelite offices. Histos, I have a question. We are a Derm POL and we recently opened a satellite office. I have been putting the satellite address on the pathology requisitions but do the path reports need to have that address or the address of the main lab where they were processed. Bacisly does CLIA want the address where the specimen was from, or the address where it was processed. Thank You, Nicole Tatum CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. ------------------------------ Message: 2 Date: Thu, 6 Aug 2015 20:27:48 +0000 From: Joanne Clark To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Manual Cassette Labelling Message-ID: <0494A7D4E8CC254EA2FB81464982E378D9ABD883 at S10MAILD001N3.SH10.lan> Content-Type: text/plain; charset="us-ascii" Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 3 Date: Thu, 6 Aug 2015 20:50:05 +0000 From: Ingles Claire To: Joanne Clark , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Manual Cassette Labelling Message-ID: Content-Type: text/plain; charset="us-ascii" We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to. Claire ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 3:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Manual Cassette Labelling Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 6 Aug 2015 13:50:58 -0700 From: "Kienitz, Kari" To: Joanne Clark , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Manual Cassette Labelling Message-ID: <41400FFE517878449D89114DD2526090196602D20F at tocmail1.tocad.orclinic.com> Content-Type: text/plain; charset="us-ascii" Epic Scientific Super Moist Markers by Creative Waste Solutions 503-657-5711 They will not let you down :) Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 1:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Manual Cassette Labelling Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 6 Aug 2015 21:03:06 +0000 From: "Mitchell, Natacha A" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Embedding Centers Message-ID: <6441A38AD60C3543B5861E7E678D0ABFB2D5DA at hhmsg02.hchd.local> Content-Type: text/plain; charset="us-ascii" My fellow histonetters, Thank you all so much for the prompt response. All the posts/emails were very helpful and I must say this site is a priceless source of knowledge, wisdom and resource!!! Natacha Mitchell CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 6 Date: Fri, 7 Aug 2015 11:35:19 +0000 From: "Boyd, Debbie M" To: Ingles Claire , Joanne Clark , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Manual Cassette Labelling Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F3F858 at TN001WEXMBX014.US.chs.net> Content-Type: text/plain; charset="us-ascii" We use KP Marker Plus from Mercedes Medical, www.mercedesmedical.com or 1-800-331-2716. Alcohol and Americlear resistant. We do not use Xylene. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Ingles Claire via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 4:50 PM To: Joanne Clark; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Manual Cassette Labelling We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to. Claire ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 3:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Manual Cassette Labelling Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 7 Date: Fri, 7 Aug 2015 14:49:38 +0000 From: "Bustamante, Lin" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] FW: Hyde-Away Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC901560C1ECF at CVMMB02.cvm.tamu.edu> Content-Type: text/plain; charset="utf-8" Can someone help her please? Thank you very much. Lin Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From: Coral Gonzalez [mailto:rayengonzalez at yahoo.com] Sent: Friday, August 07, 2015 9:28 AM To: Bustamante, Lin Subject: Hyde-Away Hello, Please when you can, ask on Histonet about the ppm legal limit for draining formalin neutralized with Hyde Away. Thank you, Rayen Gonzalez B.S., HT (ASCP) ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 141, Issue 7 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From tbraud at holyredeemer.com Fri Aug 7 13:04:06 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 7 Aug 2015 18:04:06 +0000 Subject: [Histonet] formalin limits for drain disposal Message-ID: <48E053DDF6CE074DB6A7414BA05403F8025728@HRHEX02-HOS.holyredeemer.local> The regulations vary from city to city and are managed at the municipal level. It is usually set and managed by the city chemist or the head of the EPA (Environmental Protection Agency) for your area. It can also vary when compared to the total volume sewage discharged from your facility. Believe it or not, in some places, small amounts can be approved for drain disposal without any neutralization. Make friends with your city EPA rep. It can be an invaluable relationship, and usually they will appreciate your effort to be compliant. Regards, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 From amylee053 at gmail.com Fri Aug 7 17:46:44 2015 From: amylee053 at gmail.com (Amy Lee) Date: Fri, 7 Aug 2015 15:46:44 -0700 Subject: [Histonet] rodent heart tissue harvesting Message-ID: Hello histonet, My coworker wants me to ask experts here the best way to use Potassium Chloride during rodent heart harvesting. Could any one share your procedure? We searched around on internet but not much help. If there's any way other than Potassium Chloride to relax heart muscle, please advice too. Thanks, Amy From KSimeone at leavittmgt.com Mon Aug 10 07:35:25 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 10 Aug 2015 12:35:25 +0000 Subject: [Histonet] FT NIGHT (evening shift) POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DC98D@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann at leavittmgt.com if interested. *full time position Mon-Fri or Sun-Thurs 6PM-2:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of your shift) *MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From plucas at biopath.org Mon Aug 10 07:52:34 2015 From: plucas at biopath.org (Paula) Date: Mon, 10 Aug 2015 05:52:34 -0700 Subject: [Histonet] vacation coverage Orange County, California Message-ID: <002a01d0d36b$6eb85540$4c28ffc0$@biopath.org> Hello, We need a histotech to cover a scheduled vacation starting this week, 8/12 through Wednesday, 8/19, from 5 am to about 10 am, depends on the volume. If you need to leave earlier, we can be flexible with the hours. We need help with embedding and cutting of hospital and outpatient types of tissues (broad spectrum of tissue types). Please inquire if interested at: 714-433-1330 or simply respond to this message and we'll go from there. Thank you, Paula Lucas, Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 From TanyaAbbott at catholichealth.net Mon Aug 10 08:51:26 2015 From: TanyaAbbott at catholichealth.net (Abbott, Tanya) Date: Mon, 10 Aug 2015 13:51:26 +0000 Subject: [Histonet] Slides stuck together Message-ID: <852F7D2C14FB464D80E182B15DB138AF6B80C883@CHIEX005.CHI.catholichealth.net> We found a batch of GYN slides that were filed, and have now "bonded" together. We use the Sakura coverslipper, so we are told this is not supposed to happen. I wonder if it is from residual "glue" from the coverslipping process? Anyone have any suggestions as to how to get these slides "unbonded"? Thanks in advance! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology Penn State Health St. Joseph (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From patpxs at gmail.com Mon Aug 10 09:23:27 2015 From: patpxs at gmail.com (Patpxs) Date: Mon, 10 Aug 2015 07:23:27 -0700 Subject: [Histonet] Slides stuck together In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF6B80C883@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF6B80C883@CHIEX005.CHI.catholichealth.net> Message-ID: Hi Tonya, Soak the brick-o-slides in xylene until they unstick from each other. This can take over a week, you must be patient. The slides will break if you try to force them apart. Paula Sent from my iPhone > On Aug 10, 2015, at 6:51 AM, Abbott, Tanya via Histonet wrote: > > We found a batch of GYN slides that were filed, and have now "bonded" together. We use the Sakura coverslipper, so we are told this is not supposed to happen. > I wonder if it is from residual "glue" from the coverslipping process? Anyone have any suggestions as to how to get these slides "unbonded"? > Thanks in advance! > Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > Penn State Health St. Joseph > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Mon Aug 10 10:29:32 2015 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 10 Aug 2015 11:29:32 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 8-10-2015 Here's to getting the week off to a great start!!! Message-ID: <015c01d0d381$5b637130$122a5390$@earthlink.net> Hi Histopeeps!!! I hope you had a nice weekend and are looking forward to a great week!. I have some exciting opportunities that I want to pass along as. Maybe they will look good to you or someone you know. RELIA'S Hot Histology Opportunities!! HISTOLOGY SUPERVISOR, FLAGSTAFF, AZ Histology Supervisor - Dallas, TX AP Manager - Hammond, IN Lead Histotech - Great Falls, MT HISTOTECHNICIAN/HISTOTECHNOLOGIST - VIRGINIA BEACH, VA $15K SIGN-ON BONUS!!!! Histotechnician/Histotechnologist - Atlanta, GA Lead IHC Tech - Waynesboro, VA Histotechnician - Hammond, IN Lead Histology Tech - Waco, TX Histology Tech - Montgomery, AL HISTOTECHNICIAN - KINGSPORT, TN All of these jobs are full time permanent positions with some of the finest facilities in the country. My clients offer excellent compensation and benefits and most offer relocation assistance or sign- on bonuses. For more information for you or a friend please call me at the office at 866-607-3542 or on my cell at 407-353-5070 or shoot me at email at relia1 at earthlink.net Have a great week!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tgenade at gmail.com Mon Aug 10 11:12:50 2015 From: tgenade at gmail.com (Tyrone Genade) Date: Mon, 10 Aug 2015 11:12:50 -0500 Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate? Message-ID: Hello, Last week a I visited Pittsburgh and had a chance to talk with a fellow histologist there. He remarked on the dire state his lab is in with respect to finding qualified histologists to employ; and that the lab was now considering hiring B.Sc. graduates and training them up in sectioning etc... Is this a common problem, the trouble finding qualified histotechs, and are other labs also considering hiring B.Sc. graduates to staff their labs? I have one student working with me (with medical school aspirations) who sections beautifully. There is surely talent out there... Bye -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From bcoleman at wustl.edu Mon Aug 10 12:43:41 2015 From: bcoleman at wustl.edu (Coleman, Billy) Date: Mon, 10 Aug 2015 17:43:41 +0000 Subject: [Histonet] Histonet Digest, Vol 141, Issue 8 In-Reply-To: References: Message-ID: <1439228621176.79328@wustl.edu> FYI we have the blocks. ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Saturday, August 8, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 141, Issue 8 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Histonet Digest, Vol 141, Issue 7 topic 2 (Brent Adams) 2. formalin limits for drain disposal (Terri Braud) 3. rodent heart tissue harvesting (Amy Lee) ---------------------------------------------------------------------- Message: 1 Date: Fri, 7 Aug 2015 17:29:22 +0000 From: Brent Adams To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 141, Issue 7 topic 2 Message-ID: Content-Type: text/plain; charset="Windows-1252" I had the same problem with StatLab markers. I would have issues when I let tissue set in formalin bottles all night. I would skip the formalin steps on the processor and go straight to 70% alcohol. This would almost completely erase the writing on the cassettes. The product manager informed me The Statmark Pens are made with an aqueous ink and if exposed to fingers prints or other solutions prior to being set by submerged in 100% Alcohol they will be erased or badly smudged. I keep a container of 100% alcohol on my grossing bench and dip the cassettes first before adding tissue from the containers. by time I add the tissue and close the cassette lid the alcohol has evaporated and locks the ink into the plastic cassette. If they are still slightly wet or you have alcohol on your hands they will still smudge so be careful handling cassettes. I have not had issues since going to this process. Brent Adams ? HT, BS, LPN, www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, August 7, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 141, Issue 7 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Clia requirements on specimens collected from satelite offices. (White, Marcia) 2. Manual Cassette Labelling (Joanne Clark) 3. Re: Manual Cassette Labelling (Ingles Claire) 4. Re: Manual Cassette Labelling (Kienitz, Kari) 5. Embedding Centers (Mitchell, Natacha A) 6. Re: Manual Cassette Labelling (Boyd, Debbie M) 7. FW: Hyde-Away (Bustamante, Lin) ---------------------------------------------------------------------- Message: 1 Date: Thu, 6 Aug 2015 17:01:25 +0000 From: "White, Marcia" To: "'Nicole Tatum'" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Clia requirements on specimens collected from satelite offices. Message-ID: <8B4B5BF3FCB6D24CBEAA63ACA5FDAD3B514931AE at MHSEXMB04.mhs.net> Content-Type: text/plain; charset="us-ascii" Where it was processed and diagnosis rendered Marcia M White Pathology Manager Memorial Regional Hospital 954-265-5371-office 954-967-7627-fax MWhite at mhs.net -----Original Message----- From: Nicole Tatum [mailto:nicole at dlcjax.com] Sent: Thursday, August 06, 2015 12:05 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Clia requirements on specimens collected from satelite offices. Histos, I have a question. We are a Derm POL and we recently opened a satellite office. I have been putting the satellite address on the pathology requisitions but do the path reports need to have that address or the address of the main lab where they were processed. Bacisly does CLIA want the address where the specimen was from, or the address where it was processed. Thank You, Nicole Tatum CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. ------------------------------ Message: 2 Date: Thu, 6 Aug 2015 20:27:48 +0000 From: Joanne Clark To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Manual Cassette Labelling Message-ID: <0494A7D4E8CC254EA2FB81464982E378D9ABD883 at S10MAILD001N3.SH10.lan> Content-Type: text/plain; charset="us-ascii" Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 3 Date: Thu, 6 Aug 2015 20:50:05 +0000 From: Ingles Claire To: Joanne Clark , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Manual Cassette Labelling Message-ID: Content-Type: text/plain; charset="us-ascii" We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to. Claire ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 3:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Manual Cassette Labelling Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 6 Aug 2015 13:50:58 -0700 From: "Kienitz, Kari" To: Joanne Clark , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Manual Cassette Labelling Message-ID: <41400FFE517878449D89114DD2526090196602D20F at tocmail1.tocad.orclinic.com> Content-Type: text/plain; charset="us-ascii" Epic Scientific Super Moist Markers by Creative Waste Solutions 503-657-5711 They will not let you down :) Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 1:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Manual Cassette Labelling Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 6 Aug 2015 21:03:06 +0000 From: "Mitchell, Natacha A" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Embedding Centers Message-ID: <6441A38AD60C3543B5861E7E678D0ABFB2D5DA at hhmsg02.hchd.local> Content-Type: text/plain; charset="us-ascii" My fellow histonetters, Thank you all so much for the prompt response. All the posts/emails were very helpful and I must say this site is a priceless source of knowledge, wisdom and resource!!! Natacha Mitchell CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 6 Date: Fri, 7 Aug 2015 11:35:19 +0000 From: "Boyd, Debbie M" To: Ingles Claire , Joanne Clark , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Manual Cassette Labelling Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F3F858 at TN001WEXMBX014.US.chs.net> Content-Type: text/plain; charset="us-ascii" We use KP Marker Plus from Mercedes Medical, www.mercedesmedical.com or 1-800-331-2716. Alcohol and Americlear resistant. We do not use Xylene. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Ingles Claire via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 4:50 PM To: Joanne Clark; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Manual Cassette Labelling We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to. Claire ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, August 06, 2015 3:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Manual Cassette Labelling Hi Histoland, We have a satellite lab that manually prepares their cassettes. We have had significant problems with the markers coming off after tissue processing. We have tried StatLab markers, Leica Markers and the red AquaRellable pencils and continue to have issues. Other than purchasing a cassette labeling system (their volumes are pretty low and there is limited bench space in the lab for a cassette labeler) does anyone have any suggestions or ideas on what might be causing this phenomenon or a really good permanent lab marker that has been successful for you? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 7 Date: Fri, 7 Aug 2015 14:49:38 +0000 From: "Bustamante, Lin" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] FW: Hyde-Away Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC901560C1ECF at CVMMB02.cvm.tamu.edu> Content-Type: text/plain; charset="utf-8" Can someone help her please? Thank you very much. Lin Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From: Coral Gonzalez [mailto:rayengonzalez at yahoo.com] Sent: Friday, August 07, 2015 9:28 AM To: Bustamante, Lin Subject: Hyde-Away Hello, Please when you can, ask on Histonet about the ppm legal limit for draining formalin neutralized with Hyde Away. Thank you, Rayen Gonzalez B.S., HT (ASCP) ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 141, Issue 7 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. ------------------------------ Message: 2 Date: Fri, 7 Aug 2015 18:04:06 +0000 From: "Terri Braud" To: "histonet at lists.utsouthwestern.edu" Cc: "rayengonzalez at yahoo.com" Subject: [Histonet] formalin limits for drain disposal Message-ID: <48E053DDF6CE074DB6A7414BA05403F8025728 at HRHEX02-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" The regulations vary from city to city and are managed at the municipal level. It is usually set and managed by the city chemist or the head of the EPA (Environmental Protection Agency) for your area. It can also vary when compared to the total volume sewage discharged from your facility. Believe it or not, in some places, small amounts can be approved for drain disposal without any neutralization. Make friends with your city EPA rep. It can be an invaluable relationship, and usually they will appreciate your effort to be compliant. Regards, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 ------------------------------ Message: 3 Date: Fri, 7 Aug 2015 15:46:44 -0700 From: Amy Lee To: histonet at lists.utsouthwestern.edu Subject: [Histonet] rodent heart tissue harvesting Message-ID: Content-Type: text/plain; charset=UTF-8 Hello histonet, My coworker wants me to ask experts here the best way to use Potassium Chloride during rodent heart harvesting. Could any one share your procedure? We searched around on internet but not much help. If there's any way other than Potassium Chloride to relax heart muscle, please advice too. Thanks, Amy ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 141, Issue 8 **************************************** From gmartin at marshallmedical.org Thu Aug 6 12:38:58 2015 From: gmartin at marshallmedical.org (Martin, Gary) Date: Thu, 6 Aug 2015 10:38:58 -0700 Subject: [Histonet] Leica EG1160 Message-ID: <6ED9D4252F278841A0593D3D788AF24C2E2602F7@mailsvr.MARSHMED.local> I am considering buying a refurbished Leica EG1160 and was hoping I could get some comments for everyone on its quality. Thanks Gary From srishan at mail.holyname.org Mon Aug 10 12:50:38 2015 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Mon, 10 Aug 2015 13:50:38 -0400 Subject: [Histonet] free standing cryostat heat extractor Message-ID: Can some one provide me information regarding free standing heat extractor for the cryostat. I am trying to order some extra due to increased frozen sections Thank you Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 6328 Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From Michael.LaFriniere at ccplab.com Mon Aug 10 13:39:29 2015 From: Michael.LaFriniere at ccplab.com (Michael LaFriniere) Date: Mon, 10 Aug 2015 18:39:29 +0000 Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate? In-Reply-To: References: Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29743287@AHCMSASEXCH02.my.ahc.local> Yes this is a common problem, although I am not sure "desperate enough" would be appropriate " verbiage". As histologists over the years we have learned to cope with and being highly resourceful. I have also resorted to the same idea, hiring both AS and BS individuals filling positions and training specific technical task to meet the production needs at present has worked very well in my laboratory. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041?Bournefield Way, Suite?A . Silver Spring, MD 20904?? P:?240.471.3427?. F:?240.471.3401 . Cell 410-940-8844 michael.lafriniere at ccplab.com -----Original Message----- From: Tyrone Genade via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, August 10, 2015 12:13 PM To: histonet Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate? Hello, Last week a I visited Pittsburgh and had a chance to talk with a fellow histologist there. He remarked on the dire state his lab is in with respect to finding qualified histologists to employ; and that the lab was now considering hiring B.Sc. graduates and training them up in sectioning etc... Is this a common problem, the trouble finding qualified histotechs, and are other labs also considering hiring B.Sc. graduates to staff their labs? I have one student working with me (with medical school aspirations) who sections beautifully. There is surely talent out there... Bye -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles at uwhealth.org Mon Aug 10 15:05:19 2015 From: CIngles at uwhealth.org (Ingles Claire) Date: Mon, 10 Aug 2015 20:05:19 +0000 Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate? In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D29743287@AHCMSASEXCH02.my.ahc.local> References: , <4A2A16B9707CE04E9CB6C82DC18C1D29743287@AHCMSASEXCH02.my.ahc.local> Message-ID: We have had this problem for years. We are more than willing to train someone. We are a small lab so a bigger concern is if they will fit in with the rest of us personality wise. Not that we just hire anyone willy-nilly, but many people with BS's have some lab experience/background anyway. We just make sure they have at least a BS so they fit the qualifications to gross. I am actually the only one in a lab of 4 with my certification, and though I also have an AA in Histotechnology, my BS in Biology made getting the AA alot easier. It is more of a concern if they can't troubleshoot and adapt on the fly to the ever changing situations in the lab. We actually just hired an HT with 28 years of experience, but don't know if she is going to make it past her 6 month eval. She can't multitask and has a hard time adapting to the different specimen types. I think she has worked mostly in research in the past. (not belittling you researchers out there, but many of you know that research vs. clinical work can take different skill sets.) Claire ________________________________________ From: Michael LaFriniere via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, August 10, 2015 1:39 PM To: tgenade at gmail.com Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] are you desperate enough to hire a B.Sc. graduate? Yes this is a common problem, although I am not sure "desperate enough" would be appropriate " verbiage". As histologists over the years we have learned to cope with and being highly resourceful. I have also resorted to the same idea, hiring both AS and BS individuals filling positions and training specific technical task to meet the production needs at present has worked very well in my laboratory. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A . Silver Spring, MD 20904 P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844 michael.lafriniere at ccplab.com -----Original Message----- From: Tyrone Genade via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, August 10, 2015 12:13 PM To: histonet Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate? Hello, Last week a I visited Pittsburgh and had a chance to talk with a fellow histologist there. He remarked on the dire state his lab is in with respect to finding qualified histologists to employ; and that the lab was now considering hiring B.Sc. graduates and training them up in sectioning etc... Is this a common problem, the trouble finding qualified histotechs, and are other labs also considering hiring B.Sc. graduates to staff their labs? I have one student working with me (with medical school aspirations) who sections beautifully. There is surely talent out there... Bye -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson at pathology-associates.com Tue Aug 11 10:02:09 2015 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Tue, 11 Aug 2015 15:02:09 +0000 Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate? In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D29743287@AHCMSASEXCH02.my.ahc.local> References: <4A2A16B9707CE04E9CB6C82DC18C1D29743287@AHCMSASEXCH02.my.ahc.local> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D9EFA3@PAEXCH1.PathologyAssociates.local> I would love to have some talented staff with degrees! I have a staff of around 22 people but I am currently the only HTL (I have a BA in Biology). I do not have anyone else who is even eligible to sit for the HTL exam. It would be very nice to have someone to share the load with! A friend of mine went to civil engineering school. He said that firms would hire engineers for management positions because it was a whole lot easier to teach an engineer the fine points of management than it was to teach engineering to a manager! The same applies to Histology- it is a lot easier to teach Histology to someone who already has a science background. I currently have one trainee who has taken many college science courses and his training has been a breeze so far. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Michael LaFriniere via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, August 10, 2015 11:39 AM To: tgenade at gmail.com Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] are you desperate enough to hire a B.Sc. graduate? Yes this is a common problem, although I am not sure "desperate enough" would be appropriate " verbiage". As histologists over the years we have learned to cope with and being highly resourceful. I have also resorted to the same idea, hiring both AS and BS individuals filling positions and training specific technical task to meet the production needs at present has worked very well in my laboratory. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A . Silver Spring, MD 20904 P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844 michael.lafriniere at ccplab.com -----Original Message----- From: Tyrone Genade via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, August 10, 2015 12:13 PM To: histonet Subject: [Histonet] are you desperate enough to hire a B.Sc. graduate? Hello, Last week a I visited Pittsburgh and had a chance to talk with a fellow histologist there. He remarked on the dire state his lab is in with respect to finding qualified histologists to employ; and that the lab was now considering hiring B.Sc. graduates and training them up in sectioning etc... Is this a common problem, the trouble finding qualified histotechs, and are other labs also considering hiring B.Sc. graduates to staff their labs? I have one student working with me (with medical school aspirations) who sections beautifully. There is surely talent out there... Bye -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From blayjorge at gmail.com Tue Aug 11 10:20:50 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Tue, 11 Aug 2015 11:20:50 -0400 Subject: [Histonet] Listservers Anatomy and Physiology Message-ID: Listservers Anatomy and Physiology Dear Histonetters: I have been looking for free listservers emphasizing Anatomy and Physiology. Do you know of any? If "yes", please feel free to send me an email directly. Gratefully, Jorge P.S. Apologies for potential duplicate emails. -- Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From riepopp2 at gmail.com Tue Aug 11 12:57:44 2015 From: riepopp2 at gmail.com (Laurie Finch) Date: Tue, 11 Aug 2015 12:57:44 -0500 Subject: [Histonet] are you desperate to hire a BS.c graduate topic Message-ID: I think from the perspective of someone who has been looking for a job for a couple of months and has experience in both clinical and research, it depends upon the availability of well qualified individuals in the area, what the market is, as well as if individuals are willing to relocate. Some areas have more qualified bodies than others. Just my .02.... Laurie Popp BA, HT/HTL(ASCP)cm From Michael.LaFriniere at ccplab.com Tue Aug 11 13:14:55 2015 From: Michael.LaFriniere at ccplab.com (Michael LaFriniere) Date: Tue, 11 Aug 2015 18:14:55 +0000 Subject: [Histonet] Histo position In-Reply-To: References: Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D297435DB@AHCMSASEXCH02.my.ahc.local> Hello Andrea, I am not sure if the market in Ohio, I am in Maryland. But I will forward you information to the histonet to see if you obtain a connection Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041?Bournefield Way, Suite?A . Silver Spring, MD 20904?? P:?240.471.3427?. F:?240.471.3401 . Cell 410-940-8844 michael.lafriniere at ccplab.com -----Original Message----- From: Matthew Helenthal [mailto:matthew.helenthal at icloud.com] Sent: Tuesday, August 11, 2015 1:41 PM To: Michael LaFriniere Subject: Histo position Hi, my name is Andrea Helenthal and I saw your post on histo net. I was wondering if you knew of any labs hiring bachelor of science students in or near Ohio. I have my bachelors and am looking to work under a pathologist for one year then sit for the HTL cert. any help would be much appreciated. Thank you very much. Sincerely, Andrea Helenthal 310-497-1088 Sent from my iPhone From mitchell at wanpost.com Tue Aug 11 17:44:40 2015 From: mitchell at wanpost.com (Mitchell Wan) Date: Wed, 12 Aug 2015 08:44:40 +1000 Subject: [Histonet] Looking for a Cryostat in Australia Message-ID: <041501d0d487$4f8f9df0$eeaed9d0$@wanpost.com> Hi All, I'm looking to purchase a working second hand cryostat. I currently have an old Miles. Looking to upgrade. Regards Mitchell 0418 745 750 --- This email has been checked for viruses by Avast antivirus software. https://www.avast.com/antivirus From jobaluyut at gmail.com Tue Aug 11 19:45:32 2015 From: jobaluyut at gmail.com (Joanna) Date: Tue, 11 Aug 2015 17:45:32 -0700 Subject: [Histonet] Anti-roll plate Message-ID: <16B184AE-21E8-48D8-A66D-4216750A9B2F@gmail.com> I'm looking for a glass anti-roll plate (8 x 3 cm) for an old Leica cryostat with old reusable steel blade. Thanks. Joanna From jobaluyut at gmail.com Wed Aug 12 09:18:52 2015 From: jobaluyut at gmail.com (Joanna) Date: Wed, 12 Aug 2015 07:18:52 -0700 Subject: [Histonet] Anti-roll plate In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7ACDAE2@Exchange.Advancederm.net> References: <16B184AE-21E8-48D8-A66D-4216750A9B2F@gmail.com> <22BDD9AABC13E24E95D1CF064B75C4B7ACDAE2@Exchange.Advancederm.net> Message-ID: <5A076F3C-E70A-4F4C-81E2-DA3FD15E6076@gmail.com> We have a Leica CM 1850. The anti-roll plate has a metal frame around it. I tried Leica and they said they don't make them anymore in Germany. But I'll try to contact Avantik. Thanks Joanna > On Aug 12, 2015, at 6:50 AM, Anne Murvosh wrote: > > Avantik Biogroup sells the Leica anti-roll plate it's item #5260164 their # is 1-888-392-8411. They cost about $130.00. it just says 70mm w. If that's not the right size (I have a newer leica cm1850) try Leika themselves. Anne > > -----Original Message----- > From: Joanna via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, August 11, 2015 5:46 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Anti-roll plate > > I'm looking for a glass anti-roll plate (8 x 3 cm) for an old Leica cryostat with old reusable steel blade. > > Thanks. > > Joanna > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jp10 at rice.edu Wed Aug 12 13:20:43 2015 From: jp10 at rice.edu (Jennifer Connell) Date: Wed, 12 Aug 2015 13:20:43 -0500 Subject: [Histonet] paraffin sectioning possibly calcified heart valves Message-ID: <22A3F718-768C-4C4F-A028-0779F1243AE7@rice.edu> Hello, I am having trouble with sectioning of paraffin embedded human aortic valve samples. I received the embedded samples from a collaborator who had the embedding done by the pathology department at her institution. Six of the 12 samples sectioned fine but the remaining samples are developing ribbon cuts and/or are crumbling in areas when sectioned. The suggestion that calcification is causing the issue has been raised by other members of my lab. Some of the samples do appear to have calcific nodules while others may have a small bit of calcification along the valve. The samples with what appears to be calcific nodules do feel stiff at the nodules and are not being cut flat with the rest of the sample there. If I reposition the samples to a new part of the blade, the defects (ribbons or crumbling) are still in the same place or reappear in the same place quickly. I use Tissue-Tek Accu-Edge disposable high-profile microtome blades on a Lieca RM 2135 microtome. For the samples that do not have the obvious nodules - is it possible this was an embedding error and I may be able to re-embed the samples and proceed? If calcification is the issue - is it possible to de-calcify the valves after they have already been fix, dehydrated, and embedded? Or are there any other suggestions on how I could proceed? Any advice or suggestions would be greatly appreciated. Thanks. Jen -- Jennifer Petsche Connell, Ph.D. Lab Manager and Research Scientist Integrative Matrix Mechanics Laboratory Department of Bioengineering Rice University From zibdeeg at yahoo.com Wed Aug 12 19:11:18 2015 From: zibdeeg at yahoo.com (Louise H) Date: Thu, 13 Aug 2015 00:11:18 +0000 (UTC) Subject: [Histonet] are you desperate to hire a BS.c graduate Message-ID: <1721741108.3874302.1439424678070.JavaMail.yahoo@mail.yahoo.com> I am a little put off by this. ?I have a BA and spent 30+ years in a research lab 20+ doing histology as well as . ?I can do MANY things and histology from collecting mouse tissues to cutting human lungs! ?Embedding by hand and everything from plastic on an old LKB that I had to make glass knives for, to paraffin to frozen and vibratome...as well as taking my own pictures. ?So just because we are NOT "certified" from a histology program does not mean we are a "last resort. ?Most of us are not pigeonholed, and are more than capable of doing all aspects of lab work in addition to histology!? ? ? Louise ? From arunjyothisp at gmail.com Thu Aug 13 03:32:33 2015 From: arunjyothisp at gmail.com (Arun Jyothi S.P) Date: Thu, 13 Aug 2015 11:32:33 +0300 Subject: [Histonet] Evaluation of microtome Message-ID: Dear Teachers I am asked to evaluate one new microtome installed in our lab. I will be very grateful if you can help me to make an evaluation report. What are the things to note and if you have any sample evaluation report please sent me so that I can make a template from that. Thank you Arun Histo tech Dubai From j.benavides at eae.csic.es Thu Aug 13 10:10:42 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Thu, 13 Aug 2015 17:10:42 +0200 Subject: [Histonet] Opinion on PTlink from Dako In-Reply-To: References: <20150804114731.CDD1A44935B@nhs-pd1e-esg101.ad1.nhs.net> Message-ID: <55CCB372.2090805@eae.csic.es> Hi, we are thinking about incorporating the PTlink system, from Dako, in our lab. Have any of you worked with it before? Any opinion? They would be greatly appreciated Thanks a lot Cheers Julio University of Le?n Spain From amylee053 at gmail.com Thu Aug 13 12:45:30 2015 From: amylee053 at gmail.com (Amy Lee) Date: Thu, 13 Aug 2015 10:45:30 -0700 Subject: [Histonet] test Message-ID: Hello, I am just wondering if I myself will see the email I sent out to histonet. Thanks, Amy From brendal.finlay at medicalcenterclinic.com Thu Aug 13 13:13:55 2015 From: brendal.finlay at medicalcenterclinic.com (Brendal Finlay) Date: Thu, 13 Aug 2015 13:13:55 -0500 Subject: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions Message-ID: For those labs who are xylene free I have two questions: ? 1. What mounting medium do you use when coverslipping oven dried slides? 2. How are you running down and coverslipping frozen sections after staining them? ? I'd like to make sure the stains are preserved over time and my experience with aqueous mounting mediums has been that they do not preserve stains well. ? Thank you! ? Brendal Finlay, HT (ASCP)? From Jose.R.deGuzman at gunet.georgetown.edu Thu Aug 13 14:47:54 2015 From: Jose.R.deGuzman at gunet.georgetown.edu (deGuzman, Jose R) Date: Thu, 13 Aug 2015 15:47:54 -0400 Subject: [Histonet] are you desperate to hire a BS.c graduate In-Reply-To: References: Message-ID: Please correct me if I'm wrong but this has been my experience so far. I came from a research Histology background where I learned many skills that I was able to developed in the clinical side. I spent 3 years preparing for the exam because my manager in research and a co-workers failed on their first attempt- they scared me. Now, I supervise the same clinical lab that took a chance at me and perform the hiring process. I have hired both certified and not-yet-certified-but-qualified-to-take-the-BOR-exam-for-certification-within-1-year. Histology is a field where you will find a wide range of experience and skill sets. You will find individuals with years of experience with a narrow skill set and an individual with little months/years of experience but have a wide range of skill set and troubleshooting experience to boot. These variations are the direct result of what the laboratory exposed us to- large, high volume versus small volume, specialized labs. If you find someone with years of experience and wide range of skills, they probably work with you already because we don't let them go. As a supervisor, I look at the following: qualifications (must meet the minimum requirements to sit for the exam), experience, skill sets, ability to learn and adapt and fit. The first, will eliminate anybody who I cannot hire due to our hiring policy. Once you meet that first criteria, everything else is a sliding scale. Fit will out-weigh experience because you can build experience but if you can't get along with my team, there is no team. I will work with anybody to develop their skills as long as they are able to learn and adapt. I'm in an area where there's strong competition for qualified individuals and I know of only 1 school with a Histology program that's over 50 miles away. It's not easy to fill positions. So for the research Histologists out there, yes it's very different. It's very routine, not much if any variation from day to day, month to month. We have to crank the cases out and some of us get pigeonholed to just embedding or just cutting for 8-12 hours. Can we make the transition? Yes, I know because I did it. Different organizations have different hiring policies. Full package candidates (certified, experienced, skilled) are rare but it makes the on-boarding and the first 90 days so much easier. Especially in a very busy lab, bringing someone in who can contribute at the same output as the established team is a dream come true. Incomplete packages need time to develop. And what I've experienced is research histologists may know a lot more than I do and are exposed to other aspects of the lab but when it comes to getting the work done, there is a lag that requires me to step-in because the work is taking longer to complete. The learning curve is steep, it doesn't take long. It's just easier to bring in a complete package. Jose MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected. Thank you. Help conserve valuable resources - only print this email if necessary. From rjbuesa at yahoo.com Thu Aug 13 15:33:25 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 13 Aug 2015 20:33:25 +0000 (UTC) Subject: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions In-Reply-To: References: Message-ID: <744363797.4420093.1439498005653.JavaMail.yahoo@mail.yahoo.com> 1-After you oven dry your stained sections, you use the very same medium you always have used. I used Permount.2- Given the very special constrains of FS (ready for diagnoses within 20 minutes of receiving the specimen) I used an aqueous mounting medium. After the diagnosis was made, I eliminated the aqueous mounting medium, washed the stained sections ? oven dried ? coverslip with Permount.Ren? On Thursday, August 13, 2015 2:20 PM, Brendal Finlay via Histonet wrote: For those labs who are xylene free I have two questions: ? 1. What mounting medium do you use when coverslipping oven dried slides? 2. How are you running down and coverslipping frozen sections after staining them? ? I'd like to make sure the stains are preserved over time and my experience with aqueous mounting mediums has been that they do not preserve stains well. ? Thank you! ? Brendal Finlay, HT (ASCP)? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barryrittman at gmail.com Thu Aug 13 17:38:16 2015 From: barryrittman at gmail.com (Barry Rittman) Date: Thu, 13 Aug 2015 17:38:16 -0500 Subject: [Histonet] are you desperate to hire a BS.c graduate In-Reply-To: References: Message-ID: Jose brings up some good points. It seems to me that we often spend too much time talking about employing people who have a narrow field of expertise, even though such expertise is necessary to get the job done. I would suggest that we instead look at the bigger picture. Experience outside your current field of work can be very useful. In looking at the histology fields, many of the great advances have not always been made by people who have several years or decades of experience but often by individual who had very limited histology experience but had expertise in other fields such as biochemistry and physics. I do not believe that any experience no matter how far removed from the current workplace is wasted. While a bachelors degree may not prepare individuals adequately for jumping right into the field of histology, it has at the very least given them a different outlook on many things that they would not have without that degree. Fresh outlook on our work allows us to improve the work we are doing and be creative instead of automatons. I think that the field of histotechnology is always improved by having individuals of different back grounds bringing different perspectives. The most important thing is to let individuals explore any new ideas they have. I would say a lot more but my wife is just serving up a beef pie. Barry On Thu, Aug 13, 2015 at 2:47 PM, deGuzman, Jose R via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Please correct me if I'm wrong but this has been my experience so far. > > I came from a research Histology background where I learned many skills > that I was able to developed in the clinical side. I spent 3 years > preparing for the exam because my manager in research and a co-workers > failed on their first attempt- they scared me. Now, I supervise the same > clinical lab that took a chance at me and perform the hiring process. I > have hired both certified and > not-yet-certified-but-qualified-to-take-the-BOR-exam-for-certification-within-1-year. > > Histology is a field where you will find a wide range of experience and > skill sets. You will find individuals with years of experience with a > narrow skill set and an individual with little months/years of experience > but have a wide range of skill set and troubleshooting experience to boot. > These variations are the direct result of what the laboratory exposed us > to- large, high volume versus small volume, specialized labs. If you find > someone with years of experience and wide range of skills, they probably > work with you already because we don't let them go. > > As a supervisor, I look at the following: qualifications (must meet the > minimum requirements to sit for the exam), experience, skill sets, ability > to learn and adapt and fit. The first, will eliminate anybody who I cannot > hire due to our hiring policy. Once you meet that first criteria, > everything else is a sliding scale. Fit will out-weigh experience because > you can build experience but if you can't get along with my team, there is > no team. I will work with anybody to develop their skills as long as they > are able to learn and adapt. I'm in an area where there's strong > competition for qualified individuals and I know of only 1 school with a > Histology program that's over 50 miles away. It's not easy to fill > positions. So for the research Histologists out there, yes it's very > different. It's very routine, not much if any variation from day to day, > month to month. We have to crank the cases out and some of us get > pigeonholed to just embedding or just cutting for 8-12 hours. Can we make > the transition? Yes, I know because I did it. > > Different organizations have different hiring policies. Full package > candidates (certified, experienced, skilled) are rare but it makes the > on-boarding and the first 90 days so much easier. Especially in a very busy > lab, bringing someone in who can contribute at the same output as the > established team is a dream come true. Incomplete packages need time to > develop. And what I've experienced is research histologists may know a lot > more than I do and are exposed to other aspects of the lab but when it > comes to getting the work done, there is a lag that requires me to step-in > because the work is taking longer to complete. The learning curve is steep, > it doesn't take long. It's just easier to bring in a complete package. > > Jose > > > > MedStar Health is a not-for-profit, integrated healthcare delivery system, > the largest in Maryland and the Washington, D.C., region. Nationally > recognized for clinical quality in heart, orthopaedics, cancer and GI. > IMPORTANT: This e-mail (including any attachments) may contain information > that is private, confidential, or protected by attorney-client or other > privilege. If you received this e-mail in error, please delete it from your > system without copying it and notify sender by reply e-mail, so that our > records can be corrected. Thank you. Help conserve valuable resources - > only print this email if necessary. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rascal_40 at hotmail.com Thu Aug 13 23:38:06 2015 From: rascal_40 at hotmail.com (Karen Stephanson) Date: Thu, 13 Aug 2015 22:38:06 -0600 Subject: [Histonet] paraffin sectioning possibly calcified heart valves Message-ID: Have you tried using RDO on the block surface? On Aug 12, 2015 12:30 PM, Jennifer Connell via Histonet wrote: Hello, I am having trouble with sectioning of paraffin embedded human aortic valve samples. I received the embedded samples from a collaborator who had the embedding done by the pathology department at her institution. Six of the 12 samples sectioned fine but the remaining samples are developing ribbon cuts and/or are crumbling in areas when sectioned. The suggestion that calcification is causing the issue has been raised by other members of my lab. Some of the samples do appear to have calcific nodules while others may have a small bit of calcification along the valve. The samples with what appears to be calcific nodules do feel stiff at the nodules and are not being cut flat with the rest of the sample there. If I reposition the samples to a new part of the blade, the defects (ribbons or crumbling) are still in the same place or reappear in the same place quickly. I use Tissue-Tek Accu-Edge disposable high-profile microtome blades on a Lieca RM 2135 microtome. For the samples that do not have the obvious nodules - is it possible this was an embedding error and I may be able to re-embed the samples and proceed? If calcification is the issue - is it possible to de-calcify the valves after they have already been fix, dehydrated, and embedded? Or are there any other suggestions on how I could proceed? Any advice or suggestions would be greatly appreciated. Thanks. Jen -- Jennifer Petsche Connell, Ph.D. Lab Manager and Research Scientist Integrative Matrix Mechanics Laboratory Department of Bioengineering Rice University _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay at medicalcenterclinic.com Fri Aug 14 10:42:53 2015 From: brendal.finlay at medicalcenterclinic.com (Brendal Finlay) Date: Fri, 14 Aug 2015 10:42:53 -0500 Subject: [Histonet] Xylene Free Labs - One Final Question References: Message-ID: <446e65b936d519529a0fbf07f092169a@medicalcenterclinic.com> Thank you for the responses to my previous questions.? One final question: ? What suppliers sell mineral oil by the gallon? I've seen it online at a variety of prices, but my regular vendors don't seem to sell it by the gallon.?If you are a vendor?and offer?it by the gallon, you may send me a quote, but please do not add me to any mailing lists or send repetitive emails. We are not yet ready to purchase anything as this is the initial stages of consideration. ? Thank you again! ? Brendal Finlay, HT (ASCP)? -----Original Message----- From: "Rene J Buesa" To: "Brendal Finlay" , histonet at lists.utsouthwestern.edu Date: 08/13/2015 15:33 Subject: Re: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions ? 1-After you oven dry your stained sections, you use the very same medium you always have used. I used Permount. 2- Given the very special constrains of FS (ready for diagnoses within 20 minutes of receiving the specimen) I used an aqueous mounting medium. After the diagnosis was made, I eliminated the aqueous mounting medium, washed the stained sections ? oven dried ? coverslip with Permount. Ren? ? ? On Thursday, August 13, 2015 2:20 PM, Brendal Finlay via Histonet wrote: For those labs who are xylene free I have two questions: ? 1. What mounting medium do you use when coverslipping oven dried slides? 2.. How are you running down and coverslipping frozen sections after staining them? ? I'd like to make sure the stains are preserved over time and my experience with aqueous mounting mediums has been that they do not preserve stains well. ? Thank you! ? Brendal Finlay, HT (ASCP)? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? From jmcgough at clinlab.com Fri Aug 14 10:55:44 2015 From: jmcgough at clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Fri, 14 Aug 2015 09:55:44 -0600 Subject: [Histonet] GMS Control In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D297435DB@AHCMSASEXCH02.my.ahc.local> References: Message-ID: We are in need of a GMS control for fungus. We have an excellent AFB control to trade. Please contact me if you are able to help. Thanks. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough at clinlab.com www.clinlab.com From rjbuesa at yahoo.com Fri Aug 14 11:02:12 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 14 Aug 2015 16:02:12 +0000 (UTC) Subject: [Histonet] Xylene Free Labs - One Final Question In-Reply-To: <446e65b936d519529a0fbf07f092169a@medicalcenterclinic.com> References: <446e65b936d519529a0fbf07f092169a@medicalcenterclinic.com> Message-ID: <1164541658.4823241.1439568132116.JavaMail.yahoo@mail.yahoo.com> I always bought my mineral oil from Jim Coyle Associates but the brand was PENRECO and I am sure you can ask them any supplier near your lab.This is the cheapest way of buying mineral oil, otherwise (from a pharmaceutical company) it will be EXTRAORDINARILY expensive.Ren? On Friday, August 14, 2015 11:56 AM, Brendal Finlay via Histonet wrote: Thank you for the responses to my previous questions.? One final question: ? What suppliers sell mineral oil by the gallon? I've seen it online at a variety of prices, but my regular vendors don't seem to sell it by the gallon.?If you are a vendor?and offer?it by the gallon, you may send me a quote, but please do not add me to any mailing lists or send repetitive emails. We are not yet ready to purchase anything as this is the initial stages of consideration. ? Thank you again! ? Brendal Finlay, HT (ASCP)? -----Original Message----- From: "Rene J Buesa" To: "Brendal Finlay" , histonet at lists.utsouthwestern.edu Date: 08/13/2015 15:33 Subject: Re: [Histonet] Xylene Free Labs - Coverslipping and Frozen Section Questions ? 1-After you oven dry your stained sections, you use the very same medium you always have used. I used Permount. 2- Given the very special constrains of FS (ready for diagnoses within 20 minutes of receiving the specimen) I used an aqueous mounting medium. After the diagnosis was made, I eliminated the aqueous mounting medium, washed the stained sections ? oven dried ? coverslip with Permount. Ren? ? ? On Thursday, August 13, 2015 2:20 PM, Brendal Finlay via Histonet wrote: For those labs who are xylene free I have two questions: ? 1. What mounting medium do you use when coverslipping oven dried slides? 2.. How are you running down and coverslipping frozen sections after staining them? ? I'd like to make sure the stains are preserved over time and my experience with aqueous mounting mediums has been that they do not preserve stains well. ? Thank you! ? Brendal Finlay, HT (ASCP)? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ? ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade at gmail.com Fri Aug 14 12:14:12 2015 From: tgenade at gmail.com (Tyrone Genade) Date: Fri, 14 Aug 2015 12:14:12 -0500 Subject: [Histonet] protocol for H&E staining of Lewy Bodies Message-ID: Hello, I'm currently using H&E to stain sections for Lewy bodies. I'm using Sigma H&E solutions ( http://www.sigmaaldrich.com/catalog/product/sigma/ht110316?lang=en®ion=US and http://www.sigmaaldrich.com/catalog/product/sigma/mhs16?lang=en®ion=US). The sections are immersed in the solutions 5 min for H and 4 min for E. At 4 min the staining was very intense so we tried it at 1 min which gave nice bright collagen and erythrocyte in my fish sections. (Tissues were fixed in Davidson's Fluid and paraffin imbedded.) We are now going to try 2 minutes... The H is also very intense so I will try a shorter staining period as well. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From CDavis at che-east.org Fri Aug 14 12:47:06 2015 From: CDavis at che-east.org (Davis, Cassie) Date: Fri, 14 Aug 2015 13:47:06 -0400 Subject: [Histonet] Can't find good help these days.... Message-ID: I graduated from a associates degree program in 1991 at a community college that has graduated an average of 6 students a year and the Mid-Atlantic market gets saturated with them. Every May these students are hungry for local jobs and many employers hire the newbies at a low salary (because they know they can), the students work at the first job long enough to get experience and their certification and move on to better paying jobs. I'm not sure what the solution is to fill the empty jobs in your area, but I do know there are histotechs looking for jobs out here. Cassandra Davis Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From aeck at dh.org Fri Aug 14 13:44:42 2015 From: aeck at dh.org (Eck, Allison) Date: Fri, 14 Aug 2015 18:44:42 +0000 Subject: [Histonet] pathologic waste for research Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A971A07C9@DH-MAIL01.dhorg.org> Does anyone know if there are companies that take pathologic waste for research purposes? Thanks Allison From naje1972 at yahoo.com Fri Aug 14 22:20:01 2015 From: naje1972 at yahoo.com (naje1972) Date: Fri, 14 Aug 2015 22:20:01 -0500 Subject: [Histonet] pathologic waste for research Message-ID: <8msqskkf99v5ojsw4ty6isnb.1439608499160@email.android.com> What type of waste are you speaking of? My company removes old paraffin blocks and microscope slides. Some for disposal. Some for research purposes.? I can be contacted at naje1972 at yahoo.com My name is Cynthia James Sent from my Verizon Wireless 4G LTE Tablet -------- Original message -------- From: "Eck, Allison via Histonet" Date: 08/14/2015 1:44 PM (GMT-06:00) To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] pathologic waste for research Does? anyone know if there are companies that take pathologic waste for research purposes? Thanks Allison _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston at nationwidechildrens.org Mon Aug 17 08:44:57 2015 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Mon, 17 Aug 2015 13:44:57 +0000 Subject: [Histonet] hepatocellular carcinoma Message-ID: Is anyone able to spare a block of hepatocellular carcinoma? Can swop with CMV, Adenovirus, RSV control blocks? Thanks Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From kdonadio at ymail.com Mon Aug 17 13:03:01 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Mon, 17 Aug 2015 18:03:01 +0000 (UTC) Subject: [Histonet] The Fun of cutting silicon from a breast transplant capsule Message-ID: <483121866.3787907.1439834581994.JavaMail.yahoo@mail.yahoo.com> Hello Histonetters, ????????????????????????????? I am curious if anyone else has had this kind of fun. Having a portion of the silicon breast capsule submitted for processing and cutting( not tissue, capsule). How did that work out? ?Would love to hear some responses on this as I just had a load of fun with one of these. :) ?Thanks ?Kim D From richard.wild at wanadoo.fr Tue Aug 18 03:51:11 2015 From: richard.wild at wanadoo.fr (richard wild) Date: Tue, 18 Aug 2015 10:51:11 +0200 Subject: [Histonet] spectra-tech stage controller Message-ID: <55D2F1FF.6060703@wanadoo.fr> Hi Could someone help about a spectra-tech stage controller (model 0042-445 or else) : It works fine - I can manage the joystick (biut with a little range problem) But I dont know how to use other functions. Any manual ? Any advice ? Best Richard From Ronald.Houston at nationwidechildrens.org Tue Aug 18 07:53:32 2015 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Tue, 18 Aug 2015 12:53:32 +0000 Subject: [Histonet] hepatocellular carcinoma Message-ID: As I didn't get any bites yesterday, let me try another approach. Can anyone help with a couple of unstained sections from hepatocellular carcinoma? We are investigating an antibody that we hope will help in differentiating hepatoblastoma (a rare malignant tumor of the liver in children) from hepatocellular carcinoma. We have more than enough hepatoblastoma cases, but few HCC Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From annamhuntley at gmail.com Tue Aug 18 08:11:34 2015 From: annamhuntley at gmail.com (Anna Huntley Coffey) Date: Tue, 18 Aug 2015 09:11:34 -0400 Subject: [Histonet] HTL Career Path Message-ID: Hello Histonet, I have been following this listserve for a couple years now and have found it to be a very interesting and helpful forum for sharing ideas and information among individuals with varied career histories in the histopath field. I sort of fell into histology work while working on my MS thesis in marine biology, making my own H&E slides for diagnosis of a parasite found in blue crabs. Since I graduated in 2011, I've worked mostly in research histology labs and just got my HTL certification last spring, working on my qIHC this fall. I like the histology field and have had the pleasure of working with some really great people. However, I've felt discouraged at times with what seems like a lack of opportunity for professional growth and advancement and sometimes it feels like the only way to get new experiences and higher pay is to go back to school for another degree or completely change jobs and move to a new institution. I realize that I got into histology almost accidentally and that some of my experiences may be unique to the research field, but I'd be very interested to hear about your experiences regarding research vs. clinical work, how you were able to be intentional about your career path, what advancement opportunities you've found, etc. if you're willing to share them. Thanks, Anna From relia1 at earthlink.net Tue Aug 18 09:10:11 2015 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 18 Aug 2015 10:10:11 -0400 Subject: [Histonet] The National Society of Histotechnology annual meeting is next week in DC!! Message-ID: <00f101d0d9bf$9a9e4930$cfdadb90$@earthlink.net> Hi Histonetters! How are you? As you may know I do the majority of my work via email and phone so I rarely get a chance to meet people in person. That is one of the many reasons I enjoy attending the NSH conventions. I wanted to drop you a line and see if you or anyone you work with is planning on attending the NSH convention in Washington DC next week? For more info on the convention here is the link: www.histoconvention.org I would love to have the opportunity to say Hi. I will be very easy to find because I am working the registration desk all day Friday and Saturday and Monday morning AND The NSH membership booth in the exhibit hall on Sunday and Monday So please stop by and say Hi! If you aren't attending this year follow the fun on FACEBOOK, INSTAGRAM and TWITTER. We will be posting with the hashtag #NSHSC We will be posting lots of pics and statuses about all the exciting stuff going on so stay tuned!! By the way. Here is a list of my current openings: HISTOLOGY SUPERVISORS/MANAGERS: Anatomic Pathology Manager - Hammond, IN Histology Manager - Dallas, TX Histology Supervisor - Dallas, TX Histology Supervisor - Flagstaff, AZ Lead Histology Tech - Atlanta, GA HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Histology Tech - Norfolk, VA - 15K sign on bonus plus relo. Dermpath Histotech - near Fayetteville, AR (Brand new Lab)!! Histotech - Seattle, WA HT/HTL- Atlanta Histotech -Montgomery, AL Histology Tech - Kingsport, TN Lead Dermpath Histotech - Waco/Brownwood, TX (Brand New Lab)!! All of these positions are full time and permanent and offer great salaries benefits and relocation or sign on bonuses. For more information please contact me at relia1 at earthlink.net or on my cell at 407-353-5070 or toll free at 866-607-3542. Hope To See You in DC!! Have a great day!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From taylor at prometheushealthcare.com Tue Aug 18 10:47:32 2015 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Tue, 18 Aug 2015 11:47:32 -0400 Subject: [Histonet] New York City- Histotechnician opportunity! Message-ID: <04bf01d0d9cd$3b2f40e0$b18dc2a0$@prometheushealthcare.com> Hello all! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. We specialize specifically in laboratory recruiting all over the United States for many hospitals and reference laboratories. We just received a new order for a great opportunity in Manhattan, NY. A well-known hospital is looking to hire a new Histotechnician for their lab. This position is a dayshift, fulltime, permanent opportunity. ASCP certification preferred and New York clinical license REQUIRED. If you may be interested in this or any of our other opportunities, please reach out to me for immediate consideration. Thank you in advance! Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office (301) 693-9057 Taylor at prometheushealthcare.com From Timothy.Morken at ucsf.edu Tue Aug 18 11:37:22 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 18 Aug 2015 16:37:22 +0000 Subject: [Histonet] FW: HTL Career Path References: Message-ID: <761E2B5697F795489C8710BCC72141FF6030C728@ex07.net.ucsf.edu> Anna, This is a great topic and you pretty much nailed it as far as getting into it "accidently." The fact is, most of us did. However, you can approach it a couple ways. The big question is, how do you want to advance. Do you want to stay in the lab and be the overall lab expert, or do you want to eventually move to some kind of management role? Either way, you need to take advantage of any opportunity that comes your way. First, if you want to stay in one institution, for whatever reason, and it is large enough, or growing, then the path to take is to first be excellent at what you currently do, and second, take advantage of every single opportunity to learn something new, including taking on special projects, committees (ie, QA, safety, emergency planning, whatever comes along) in order to get to know as many people in the organization as possible and what opportunities exist in other areas. For instance, one person here was a med tech in the clin lab from many years, took on QA duties, worked on CAP and JC inspection readiness, participated in doing CAP inspections, and now is the QA person for the entire laboratory and POC labs - which is a huge job in our institution (dozens of lab sites). So, essentially finding ways to move up. You could also do that in a management route with the idea that you learn management that can be applied to any department, not just histology or pathology. That would allow you to work anywhere. The other path is to move to other institutions as you outgrow your job at your current workplace. If your institution is small you may or may not be able to move up. A lot of times It depends more on others moving up or out rather than your own ambitions. In that case keep your options open for other opportunities that come up. Generally, if you move to a new job you should also be moving up at least to the next higher level, otherwise your pay will go down rather than up (no place will bring you in at the high pay rate you reached after 10 years in your current job - they want to bring you on in the middle of their pay range). That means you need to take advantage of learning the next level of more advanced skills in any job you have. Never stick to your job description - that is just a suggestion and should be considered the floor level of what you should do. No manger is ever going to ask you to stop volunteering to do more! Additionally, look for opportunities to apply current knowledge in new areas, whether research, government labs, including working for a vendor. You would be amazed at how much you can learn in that venue that will help you in all other aspects of your work (product development, marketing, sales, customer relations, technical support, etc). In short, learn everything you can about your field, attend meetings, even if on your own dime, get involved in management of the lab, take on special projects, move around if necessary to gain new experience and perspective. And have some fun along the way! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Anna Huntley Coffey via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 18, 2015 6:12 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HTL Career Path Hello Histonet, I have been following this listserve for a couple years now and have found it to be a very interesting and helpful forum for sharing ideas and information among individuals with varied career histories in the histopath field. I sort of fell into histology work while working on my MS thesis in marine biology, making my own H&E slides for diagnosis of a parasite found in blue crabs. Since I graduated in 2011, I've worked mostly in research histology labs and just got my HTL certification last spring, working on my qIHC this fall. I like the histology field and have had the pleasure of working with some really great people. However, I've felt discouraged at times with what seems like a lack of opportunity for professional growth and advancement and sometimes it feels like the only way to get new experiences and higher pay is to go back to school for another degree or completely change jobs and move to a new institution. I realize that I got into histology almost accidentally and that some of my experiences may be unique to the research field, but I'd be very interested to hear about your experiences regarding research vs. clinical work, how you were able to be intentional about your career path, what advancement opportunities you've found, etc. if you're willing to share them. Thanks, Anna _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From clmcmah at clemson.edu Tue Aug 18 12:13:42 2015 From: clmcmah at clemson.edu (Chad L McMahan) Date: Tue, 18 Aug 2015 17:13:42 +0000 Subject: [Histonet] SCSHT Fall Histo-Expo, Pawley's Island SC, October 23-24 Message-ID: Hello All Histonetters, We are hosting our SCSHT annual fall meeting this year in sunny Pawley's Island, SC. The Histo-Expo event will be hosted by great speakers. Please review attachments for speaker agendas, registration, and additional information concerning meeting. Please note that early bird registration for hotel ends September 30, 2015. Please consider attending the event for great workshops, networking opportunities, vendor displays, and a wonderful opportunity to fellowship with friends and meet new ones! We look forward to seeing you in October! Please contact me and I will gladly submit all information to you. I can provide speaker agendas, meeting information, lodging accommodations, and registration documents. We want you to have a great time! Have a great day, Chad McMahan clmcmah at clemson.edu [http://www.tigersports.com/productimages/imageaddons11/ovalpawmagnet180.jpg] From tejohnson at genoptix.com Tue Aug 18 13:38:28 2015 From: tejohnson at genoptix.com (Teri Johnson) Date: Tue, 18 Aug 2015 18:38:28 +0000 Subject: [Histonet] HTL Career Path Message-ID: <1c92acf1ade34e6c970dee7403612885@PHUSCB-SP37MB03.genoptix.org> Hi Anna, I really cannot beat what Tim has said in response to your concerns. He is spot on and I think his experience in some way mirrors my own (with the exception of industry experience). My question to you, though, relates to your expectation. How quickly are you expecting to advance in your career, and is that expectation reasonable? Those of us who have been in this field for a long time knows it usually takes quite a while to learn and to prove yourself to an employer. It is possible for highly motivated and high producing staff members to rise to the top fairly quickly if there is opportunity. It may be more common, though, to find yourself highly motivated and highly productive and still in your current role for longer than you want due to lack of vacancies. Honestly, I find the pay scale for HT/HTL to be quite good in most metropolitan areas and there are definitely opportunities out there to maximize your compensation provided you have a resume to support it. You've got to earn it, you've got to put in the work as Tim outlined. Learn everything you can, network, make yourself valuable, but never lose sight of the importance of teamwork and demonstrate that ability. I don't think I would go back to school and expect it to reflect in a positive way financially if you decide to stay in Histotechnology. You will end up trying to pay off a student loan with not much added compensation for your degree since it really isn't required beyond a Bachelor's degree. I do hope you remain in the field. Your current knowledge and enthusiasm for the discipline is a wonderful thing. Best wishes, Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com -----Original Message----- Anna, This is a great topic and you pretty much nailed it as far as getting into it "accidently." The fact is, most of us did. However, you can approach it a couple ways. The big question is, how do you want to advance. Do you want to stay in the lab and be the overall lab expert, or do you want to eventually move to some kind of management role? Either way, you need to take advantage of any opportunity that comes your way. First, if you want to stay in one institution, for whatever reason, and it is large enough, or growing, then the path to take is to first be excellent at what you currently do, and second, take advantage of every single opportunity to learn something new, including taking on special projects, committees (ie, QA, safety, emergency planning, whatever comes along) in order to get to know as many people in the organization as possible and what opportunities exist in other areas. For instance, one person here was a med tech in the clin lab from many years, took on QA duties, worked on CAP and JC inspection readiness, participated in doing CAP inspections, and now is the QA person for the entire laboratory and POC labs - which is a huge job in our institution (dozens of lab sites). So, essentially finding ways to move up. You could also do that in a management route with the idea that you learn management that can be applied to any department, not just histology or pathology. That would allow you to work anywhere. The other path is to move to other institutions as you outgrow your job at your current workplace. If your institution is small you may or may not be able to move up. A lot of times It depends more on others moving up or out rather than your own ambitions. In that case keep your options open for other opportunities that come up. Generally, if you move to a new job you should also be moving up at least to the next higher level, otherwise your pay will go down rather than up (no place will bring you in at the high pay rate you reached after 10 years in your current job - they want to bring you on in the middle of their pay range). That means you need to take advantage of learning the next level of more advanced skills in any job you have. Never stick to your job description - that is just a suggestion and should be considered the floor level of what you should do. No manger is ever going to ask you to stop volunteering to do more! Additionally, look for opportunities to apply current knowledge in new areas, whether research, government labs, including working for a vendor. You would be amazed at how much you can learn in that venue that will help you in all other aspects of your work (product development, marketing, sales, customer relations, technical support, etc). In short, learn everything you can about your field, attend meetings, even if on your own dime, get involved in management of the lab, take on special projects, move around if necessary to gain new experience and perspective. And have some fun along the way! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Anna Huntley Coffey via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 18, 2015 6:12 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HTL Career Path Hello Histonet, I have been following this listserve for a couple years now and have found it to be a very interesting and helpful forum for sharing ideas and information among individuals with varied career histories in the histopath field. I sort of fell into histology work while working on my MS thesis in marine biology, making my own H&E slides for diagnosis of a parasite found in blue crabs. Since I graduated in 2011, I've worked mostly in research histology labs and just got my HTL certification last spring, working on my qIHC this fall. I like the histology field and have had the pleasure of working with some really great people. However, I've felt discouraged at times with what seems like a lack of opportunity for professional growth and advancement and sometimes it feels like the only way to get new experiences and higher pay is to go back to school for another degree or completely change jobs and move to a new institution. I realize that I got into histology almost accidentally and that some of my experiences may be unique to the research field, but I'd be very interested to hear about your experiences regarding research vs. clinical work, how you were able to be intentional about your career path, what advancement opportunities you've found, etc. if you're willing to share them. Thanks, Anna ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From Linda.Margraf at cookchildrens.org Tue Aug 18 14:10:10 2015 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Tue, 18 Aug 2015 19:10:10 +0000 Subject: [Histonet] Histotech position in Arizona Message-ID: <928719B9EBFA1C4686918B975FF84528F4C3041A@CCHCSMBX04.CCHCS.LDAP> Dear Histonetters: Here is information (please see below) I am posting for Rita about a position at the University of Arizona...Please check with her at ritar at email.arizona.edu if you have questions. Thanks! Linda M, Histonet administrator Title: Histotechnologist Department: Animal & Biomedical Sciences-Res (2450) Location: Main Campus - University of Arizona, Tucson Arizona Position Summary: The School of Animal & Comparative Biomedical Sciences is currently seeking a well organized and highly motivated person for the position of Histotechnologist. The incumbent will prepare microscope slides of tissue for pathological studies and be responsible for all aspects of maintaining the histology lab. The position is focused on shrimp diseases for a diagnostic laboratory and shrimp research. Other duties may be assigned as needed. The candidate must be highly detail oriented and able to work well on their own with good organization and multi-tasking skills. Outstanding UA benefits include health, dental, and vision insurance plans; life insurance and disability programs; paid vacation, sick leave, and holidays; UA/ASU/NAU tuition reduction for the employee and qualified family members; state retirement plan; access to UA recreation and cultural activities; and more! The University of Arizona has been recognized on Forbes 2015 list of America's Best Employers in the United States and has been awarded the 2015 Work-Life Seal of Distinction by the Alliance for Work-Life Progress! For more information about working at the University of Arizona, please go to this link: http://employment.arizona.edu/ Duties & Responsibilities: * Maintain all aspects of the histology laboratory. * Perform all duties according to ISO/IEC 17025 standards. * Tissue sample preparation for histological analysis including: Dissection, paraffin infiltration, embedding, sectioning and staining. * Knowledge of ISH and IHC procedures is desirable. * Keep ISO/IEC 17025 logs of equipment maintenance, temperatures, tissue processing and staining. * Maintain the laboratory's archive of histological slides, paraffin blocks and preserved tissues. * Log samples into the FilemakerPro database upon arrival at the laboratory. * Maintain FilemakerPro database and databooks: archiving of data, organization of databooks, database maintenance. * Participate as a histotech instructor during special workshops and for graduate students and visiting scholars. Minimum Qualifications: Please see Arizona Board of Regents Minimum Qualifications. Arizona Board of Regents Minimum Qualifications Two years of college with courses in Biology and Chemistry or eligibility for registration as a Histotechnologist with the American Society of Clinical Pathologists (ASCP) AND one year of experience sectioning and staining tissue and bone marrow; OR, any equivalent combination of experience, training and/or education. Preferred Qualifications: * HT or HTL ASCP certification preferred, but will consider experience in place of certification. * Minimum of 1 year experience in paraffin block sectioning. * Knowledge of the principles and practices of tissue processing and slide development. * Ability to effectively communicate with others. * Excellent organizational and multi-tasking skills. * Knowledge of performing ISH and IHC is optional, but it would be helpful. Full Time Job Category: General Benefits Eligible: Yes - Full Benefits FLSA: Non-Exempt Posted Rate of Pay: $25,478 - $37,915 Annually Apply on-line at: https://uacareers.com/hr/postings/4542 From ADuddey at firsthealth.org Tue Aug 18 14:54:05 2015 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Tue, 18 Aug 2015 19:54:05 +0000 Subject: [Histonet] Fetal demise Message-ID: <09FBA01CA9B6374A83C5C76E09E46188126F43E8@EXMAIL1-FHC.firsthealth.org> North Carolina Histologists - Would anyone be willing to share their fetal demise and disposition policy with me? We are reviewing and there are some things that are unclear and several different referrals to NCGS that makes it even more confusing. Thanks in advance, Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FH Moore Regional Hospital 910-715-5286 office 910715-1944 fax From jerrysedgewick at gmail.com Tue Aug 18 15:37:01 2015 From: jerrysedgewick at gmail.com (J. Sedgewick) Date: Tue, 18 Aug 2015 15:37:01 -0500 Subject: [Histonet] Free webinar on Photoshop, ChromaCal Message-ID: <1D7F6C60DC024041BA4522931630DF4E@sedge> 5 Common Mistakes when using Photoshop for Science: Tips, Tricks and a Case Study Sep 9, 2015 1:00 PM EDT at: https://attendee.gotowebinar.com/register/2382965322253067778 Images are a vital component of scientific research for evaluation, data analysis, visualizing the impact of experimental parameters, and in medicine for assessing disease conditions. Regardless of the equipment price tag or level of automation, microscope systems generally fall short in providing images with consistent quality optimized for color, contrast and brightness. Adobe? Photoshop? or other image editing software is commonly used to correct for image insufficiencies, but consequences exist. Join our speakers as they review the common mistakes encountered when using Photoshop for science, offer tips to achieve consistency and high quality in your imaging process, and share their personal experience on how to avoid these common mistakes altogether. This webinar is co-sponsored by Imaging and Analysis, LLC and Datacolor Inc. About the speakers: Jerry Sedgewick (Imaging and Analysis, LLC), is an imaging consultant, an image forensic specialist, and a former core microscopy facility manager. As a recognized authority in applying Photoshop to edit scientific images, and through his books, articles and workshops, Mr. Sedgewick has helped hundreds of researchers use Photoshop to achieve their desired results. Dr. Dawn M. Dawson received her M.D. from Eastern Virginia Medical School in 1989, followed by a residency and fellowship in Anatomic and Clinical Pathology at the Cleveland Clinic Foundation, receiving Board Certification in 1996. From 1998 until 2000, Dr. Dawson was a fellow in the Translational Research in Oncology Training Program. Currently, she is an Assistant Professor at Case Western Reserve University's Institute of Pathology and her responsibilities include immunohistochemistry activities. Her current research focuses on growth factor/receptors and cell signaling in carcinogenesis models. From Linda.Margraf at cookchildrens.org Wed Aug 19 09:06:43 2015 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Wed, 19 Aug 2015 14:06:43 +0000 Subject: [Histonet] automatic stainer opinions sought Message-ID: <928719B9EBFA1C4686918B975FF84528F4C33A94@CCHCSMBX04.CCHCS.LDAP> We are a small lab looking for a small automatic stainer.? Has anyone used and have an opinion on the Sakura Histo-Tek SL automatic stainer?? ? Connie Dieringer, HTL(ASCP) Chief Histologist ? Department of Diagnostic & Biomedical Science | 7500 Cambridge Street | Suite 6110 | Houston, Texas 77054 | 713-486-4413 tel | 713-486-0415 fax connie.j.dieringer at uth.tmc.edu From zerfasp at ors.od.nih.gov Wed Aug 19 09:12:05 2015 From: zerfasp at ors.od.nih.gov (Zerfas, Patricia (NIH/OD/ORS) [E]) Date: Wed, 19 Aug 2015 14:12:05 +0000 Subject: [Histonet] microtome blade Message-ID: <997D36BD8D0ACF418AF900F4E781DE4426EE83E5@msgb04.nih.gov> Dear Histologists, After you have completed sectioning for the day do you remove the blade? Is it safer to keep the blade locked in place? Is it a safety issue? Or is it a policy set by your workplace? Thanks, Patricia M. Zerfas National Institutes of Health Bldg 28A Room 112 MSC 5230 9000 Rockville Pike Bethesda, MD 20895 USA (301) 496-4464 (301) 402-1068 From KSimeone at leavittmgt.com Wed Aug 19 11:14:02 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 19 Aug 2015 16:14:02 +0000 Subject: [Histonet] FT NIGHT (evening shift) POSITION DELRAY BCH FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C4D2DC98D@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C4D2DC98D@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DCC2B@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann at leavittmgt.com if interested. *full time position Mon-Fri or Sun-Thurs 6PM-2:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of your shift) *MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From dmecham at copc.net Wed Aug 19 12:15:30 2015 From: dmecham at copc.net (Joseph Mecham) Date: Wed, 19 Aug 2015 17:15:30 +0000 Subject: [Histonet] Job Opening In Bend, OR Message-ID: Title: Histotech Location: Central Oregon Pathology - Bend, OR Position Summary: Central Oregon Pathology is currently seeking a well-organized and highly motivated person for the position of Histotech. The candidate will prepare microscope slides of tissue for pathological studies and be responsible for all aspects of maintaining the histology lab. The position is focused on a wide range of tissue types. Other duties may be assigned as needed. The candidate must be detail oriented and able to work well on their own with good organization and multi-tasking skills. Outstanding benefits include health and dental insurance plans; paid vacation; retirement plan; and FSA. Duties & Responsibilities: * Maintain all aspects of the histology laboratory. * Tissue sample preparation for histological analysis including: Dissection, paraffin infiltration, embedding, sectioning and staining. * Knowledge of IHC procedures is desirable. * Maintain the laboratory's archive of histological slides, paraffin blocks and preserved tissues. * Log samples into the PowerPath database upon arrival at the laboratory. * Maintain PowerPath database: archiving of data, organization of databooks, database maintenance. Minimum Qualifications: ASCP Eligible and High School Diploma Preferred Qualifications: * HT or HTL ASCP certification preferred, but will consider experience in place of certification. * Knowledge of the principles and practices of tissue processing and slide development. * Ability to effectively communicate with others. * Excellent organizational and multi-tasking skills. * Knowledge of performing IHC is optional, but it would be helpful. Full Time Job Category: General Benefits Eligible: Yes - Full Benefits Posted Rate of Pay: $40,000 - $52,000 Annually Please email resume to dmecham at copc.net From jaylundgren at gmail.com Wed Aug 19 16:48:16 2015 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 19 Aug 2015 16:48:16 -0500 Subject: [Histonet] microtome blade In-Reply-To: <997D36BD8D0ACF418AF900F4E781DE4426EE83E5@msgb04.nih.gov> References: <997D36BD8D0ACF418AF900F4E781DE4426EE83E5@msgb04.nih.gov> Message-ID: NEVER leave a blade in a microtome when not sectioning! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Aug 19, 2015 at 9:12 AM, Zerfas, Patricia (NIH/OD/ORS) [E] via Histonet wrote: > Dear Histologists, > After you have completed sectioning for the day do you remove the blade? > Is it safer to keep the blade locked in place? Is it a safety issue? Or > is it a policy set by your workplace? > > Thanks, > > Patricia M. Zerfas > National Institutes of Health > Bldg 28A Room 112 MSC 5230 > 9000 Rockville Pike > Bethesda, MD 20895 USA > (301) 496-4464 > (301) 402-1068 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From craigak12 at gmail.com Wed Aug 19 17:20:47 2015 From: craigak12 at gmail.com (Jb) Date: Wed, 19 Aug 2015 15:20:47 -0700 Subject: [Histonet] Nails: Message-ID: <2663593C-C058-4D99-8D09-3053342AF205@gmail.com> Does anyone have any special tricks to keeping nail sections on the slides? I am doing a PAS stain and the tissue keeps falling off. Using + Slides, cut on 1-2 microns, baking 1/2 hr. Air drying, and this nail still wants to fall off. Any suggestions. Please help, Craig Sent from my iPhone From dunatrsd at sbcglobal.net Wed Aug 19 17:22:48 2015 From: dunatrsd at sbcglobal.net (dusko trajkovic) Date: Wed, 19 Aug 2015 22:22:48 +0000 (UTC) Subject: [Histonet] alpha V beta 3 antibody search Message-ID: <1176821842.7610146.1440022968621.JavaMail.yahoo@mail.yahoo.com> Hi Everyone, I have tried 2 different aVb3 antibodies, and they do not seem to work, or rather stain properly. Does anyone have a aVb3 antibody that will work on FFPE sections??Any info would be greatly appreciated.ThanksDusko From ian.bernard at comcast.net Wed Aug 19 20:53:32 2015 From: ian.bernard at comcast.net (ian bernard) Date: Wed, 19 Aug 2015 19:53:32 -0600 Subject: [Histonet] Freeze Spray Not Sold as Case Message-ID: Fellow Histonetters: I'm looking for a source to purchase Freeze-Spray used during the frozen procedure. Our current resource sells Freeze Spray in a case of 6 cans. I would like to purchase as an individual can owing to our workload rather than a case. Any references? Ian Bernard From richard.wild at wanadoo.fr Thu Aug 20 00:07:14 2015 From: richard.wild at wanadoo.fr (richard wild) Date: Thu, 20 Aug 2015 07:07:14 +0200 Subject: [Histonet] microscope stage controller spectra-tech Message-ID: <55D56082.6020602@wanadoo.fr> Hi Could someone help about a spectra-tech stage controller (model 0042-445 or else) : It works fine - I can manage the joystick (but with a little range problem) But I dont know how to use other functions. Any manual ? Any advice ? Best Richard From mjones at metropath.com Thu Aug 20 08:58:00 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Thu, 20 Aug 2015 13:58:00 +0000 Subject: [Histonet] Nails: In-Reply-To: <2663593C-C058-4D99-8D09-3053342AF205@gmail.com> References: <2663593C-C058-4D99-8D09-3053342AF205@gmail.com> Message-ID: After grossing in, we soak the nail in Nair (dab of HOT water mixed to runny paste) for a couple of hours to soften the nail. We face into the block, soak the face of the block in fresh Nair solution again for at least 30 minutes. Bake sections in the oven for 1 hour at least, sometimes hand stain so that slide is treated gently. Tissue can still fall of sometimes - nature of the beast! I guess they use Nair for Rhinoceros horns! Good Luck :) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 8/19/15, 4:20 PM, "Jb via Histonet" wrote: >Does anyone have any special tricks to keeping nail sections on the >slides? I am doing a PAS stain and the tissue keeps falling off. > >Using + Slides, cut on 1-2 microns, baking 1/2 hr. Air drying, and this >nail still wants to fall off. > >Any suggestions. Please help, > >Craig > >Sent from my iPhone >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin at mhhcc.org Thu Aug 20 11:28:36 2015 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Thu, 20 Aug 2015 16:28:36 +0000 Subject: [Histonet] FW: Histo tech position In-Reply-To: <9f053526d3ea41ee87d0559fcd3a09f0@exch02.mhhcc.org> References: <9f053526d3ea41ee87d0559fcd3a09f0@exch02.mhhcc.org> Message-ID: <0456ed5c1ac5425a90b565ae90028d07@exch02.mhhcc.org> From: Baldwin, Kathy Sent: Thursday, August 20, 2015 9:27 AM To: histonet at lists.utsouthwestern.edu Subject: Histo tech position MEMORIAL HOSPITAL AND HEALTH CARE CENTER IS SEEKING: Histology Tech position: Part time 32 hours per week with benefits. Jasper, Indiana 47546 Jasper is a regional center in southwestern Indiana, noted for its heavily German Catholic ancestral roots Jasper has often been called the "Wood Capital of the World", boasting a large number of furniture companies, including Kimball International and Masterbrand Cabinets. Jasper is also home to the Southern Indiana Education Service Center (SIEC), Jasper Engines & Transmissions (largest remanufacturer in the market), and to a satellite campus of Vincennes University. JOB SUMMARY: Responsible for embedding, sectioning and cover slipping paraffin blocks and staining tissues to provide the pathologist with accurately prepared specimens for review and diagnosis. The employee works with specimens from patients of all ages. The employee must reflect mission statement and philosophy of Memorial Hospital and Health Care Center in daily work habits and contacts. QUALIFICATIONS: Education: High school diploma or equivalent is required. Associate degree preferred. HT (ASCP) certification is preferred. Three years' experience as histology assist is acceptable. Training: Six months on the job training required. Experience: Previous histology experience is preferred. New graduates of histology school will be considered. Please contact me, Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From twheelock at mclean.harvard.edu Thu Aug 20 11:56:54 2015 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Thu, 20 Aug 2015 16:56:54 +0000 Subject: [Histonet] Formaldehyde sensors and badges Message-ID: <69718C0B0B3C414D9F8E7214AD400CC98C01B089@PHSX10MB11.partners.org> Hi Everyone: We are looking into formaldehyde sensors or badges to monitor our exposure to formaldehyde in our dissection room and our fixed tissue storage room. Does anyone have experience with this that they could share? Thank you so much. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From mjones at metropath.com Thu Aug 20 11:58:58 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Thu, 20 Aug 2015 16:58:58 +0000 Subject: [Histonet] HP IHC Message-ID: Good Morning, My apologies, I missed the conversations speaking about HP IHC stains previously. Has anyone used the monoclonal antibody for HP? Is it as sensitive and specific as the polyclonal antibody but without the background? We use polyclonal and are experiencing a tremendous amount of background. I've adjusted the stain and can do more, I just wondered if anyone was using monoclonal with better results? Thanks for your help! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com From tbraud at holyredeemer.com Thu Aug 20 12:32:10 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 20 Aug 2015 17:32:10 +0000 Subject: [Histonet] Freeze spray for frozen sections In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F8027D20@HRHEX02-HOS.holyredeemer.local> I have an easy answer for you. Don't use freezing spray in a cryostat. EVER. It creates dangerous airborne microdroplets that can linger and transmit disease within the room for hours. Here are 2 case documentations, and there are others. If you can't cut a section frozen at cryostat temperatures, then chill the block by other means, but please, for the sake of the safety of all, don't use freezing spray in or near a cryostat. 1) "Tuberculosis Infection Associated with Tissue Processing - California" in: Morbidity and Mortality Weekly Report, Centers for Disease Control and Prevention, US Public Health Service. Vol 30, No. 6 p.73(Feb 20, 1981)(unfortunately this issue is not available on the CDC web site). Reported by T. Barret, RN Berkeley and HA Rentein, MD, California State Dept of Health Services, in the California Morbidity Weekly Report, No. 30, Aug 1, 1980. This report describes a scenario in which two pathologists were documented to seroconvert against TB antigen three months after doing a frozen section on a TB-positive patient (baselines on both were negative at the time the patient was diagnosed). Although 17 employees were in contact with the patient, only the pathologists seroconverted. And, while only one of the pathologists was actually present at the autopsy, both were present at the cryostat when a freezing spray was used to freeze the specimen. Neither was wearing a mask. It is thought (though not definitively proved) that the spray action led to the aerosolization of the TB organism and to infection of the pathologists. 2) "TB Infection from preparation of frozen sections." (Correspondence Setion) in New England Journal of Medicine, V.305(3)p167, Duray, PH, 1981 (July 16). This is a short letter about a histotech at Norwood Hospital, Norwood, MA, who seroconverted to TB two months after doing a frozen section on a TB-positive patient (All employees have annual TB skin tests and this technologist had been negative). The histotech used a freezing spray to freeze the specimen. Again, the proof is not definitive, but is suggestive. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, August 20, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 141, Issue 18 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Job Opening In Bend, OR (Joseph Mecham) 2. Re: microtome blade (Jay Lundgren) 3. Nails: (Jb) 4. alpha V beta 3 antibody search (dusko trajkovic) 5. Freeze Spray Not Sold as Case (ian bernard) 6. microscope stage controller spectra-tech (richard wild) 7. Re: Nails: (Michael Ann Jones) 8. FW: Histo tech position (Baldwin, Kathy) 9. Formaldehyde sensors and badges (Wheelock, Timothy R.) 10. HP IHC (Michael Ann Jones) ---------------------------------------------------------------------- Message: 1 Date: Wed, 19 Aug 2015 17:15:30 +0000 From: Joseph Mecham To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Job Opening In Bend, OR Message-ID: Content-Type: text/plain; charset="us-ascii" Title: Histotech Location: Central Oregon Pathology - Bend, OR Position Summary: Central Oregon Pathology is currently seeking a well-organized and highly motivated person for the position of Histotech. The candidate will prepare microscope slides of tissue for pathological studies and be responsible for all aspects of maintaining the histology lab. The position is focused on a wide range of tissue types. Other duties may be assigned as needed. The candidate must be detail oriented and able to work well on their own with good organization and multi-tasking skills. Outstanding benefits include health and dental insurance plans; paid vacation; retirement plan; and FSA. Duties & Responsibilities: * Maintain all aspects of the histology laboratory. * Tissue sample preparation for histological analysis including: Dissection, paraffin infiltration, embedding, sectioning and staining. * Knowledge of IHC procedures is desirable. * Maintain the laboratory's archive of histological slides, paraffin blocks and preserved tissues. * Log samples into the PowerPath database upon arrival at the laboratory. * Maintain PowerPath database: archiving of data, organization of databooks, database maintenance. Minimum Qualifications: ASCP Eligible and High School Diploma Preferred Qualifications: * HT or HTL ASCP certification preferred, but will consider experience in place of certification. * Knowledge of the principles and practices of tissue processing and slide development. * Ability to effectively communicate with others. * Excellent organizational and multi-tasking skills. * Knowledge of performing IHC is optional, but it would be helpful. Full Time Job Category: General Benefits Eligible: Yes - Full Benefits Posted Rate of Pay: $40,000 - $52,000 Annually Please email resume to dmecham at copc.net ------------------------------ Message: 2 Date: Wed, 19 Aug 2015 16:48:16 -0500 From: Jay Lundgren To: "Zerfas, Patricia (NIH/OD/ORS) [E]" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] microtome blade Message-ID: Content-Type: text/plain; charset=UTF-8 NEVER leave a blade in a microtome when not sectioning! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Aug 19, 2015 at 9:12 AM, Zerfas, Patricia (NIH/OD/ORS) [E] via Histonet wrote: > Dear Histologists, > After you have completed sectioning for the day do you remove the blade? > Is it safer to keep the blade locked in place? Is it a safety issue? > Or is it a policy set by your workplace? > > Thanks, > > Patricia M. Zerfas > National Institutes of Health > Bldg 28A Room 112 MSC 5230 > 9000 Rockville Pike > Bethesda, MD 20895 USA > (301) 496-4464 > (301) 402-1068 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Wed, 19 Aug 2015 15:20:47 -0700 From: Jb To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Nails: Message-ID: <2663593C-C058-4D99-8D09-3053342AF205 at gmail.com> Content-Type: text/plain; charset=us-ascii Does anyone have any special tricks to keeping nail sections on the slides? I am doing a PAS stain and the tissue keeps falling off. Using + Slides, cut on 1-2 microns, baking 1/2 hr. Air drying, and this nail still wants to fall off. Any suggestions. Please help, Craig Sent from my iPhone ------------------------------ Message: 4 Date: Wed, 19 Aug 2015 22:22:48 +0000 (UTC) From: dusko trajkovic To: Histonet Subject: [Histonet] alpha V beta 3 antibody search Message-ID: <1176821842.7610146.1440022968621.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hi Everyone, I have tried 2 different aVb3 antibodies, and they do not seem to work, or rather stain properly. Does anyone have a aVb3 antibody that will work on FFPE sections??Any info would be greatly appreciated.ThanksDusko ------------------------------ Message: 5 Date: Wed, 19 Aug 2015 19:53:32 -0600 From: "ian bernard" To: Subject: [Histonet] Freeze Spray Not Sold as Case Message-ID: Content-Type: text/plain; charset="us-ascii" Fellow Histonetters: I'm looking for a source to purchase Freeze-Spray used during the frozen procedure. Our current resource sells Freeze Spray in a case of 6 cans. I would like to purchase as an individual can owing to our workload rather than a case. Any references? Ian Bernard ------------------------------ Message: 6 Date: Thu, 20 Aug 2015 07:07:14 +0200 From: richard wild To: histonet at lists.utsouthwestern.edu Subject: [Histonet] microscope stage controller spectra-tech Message-ID: <55D56082.6020602 at wanadoo.fr> Content-Type: text/plain; charset=utf-8; format=flowed Hi Could someone help about a spectra-tech stage controller (model 0042-445 or else) : It works fine - I can manage the joystick (but with a little range problem) But I dont know how to use other functions. Any manual ? Any advice ? Best Richard ------------------------------ Message: 7 Date: Thu, 20 Aug 2015 13:58:00 +0000 From: Michael Ann Jones To: Jb , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Nails: Message-ID: Content-Type: text/plain; charset="us-ascii" After grossing in, we soak the nail in Nair (dab of HOT water mixed to runny paste) for a couple of hours to soften the nail. We face into the block, soak the face of the block in fresh Nair solution again for at least 30 minutes. Bake sections in the oven for 1 hour at least, sometimes hand stain so that slide is treated gently. Tissue can still fall of sometimes - nature of the beast! I guess they use Nair for Rhinoceros horns! Good Luck :) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 8/19/15, 4:20 PM, "Jb via Histonet" wrote: >Does anyone have any special tricks to keeping nail sections on the >slides? I am doing a PAS stain and the tissue keeps falling off. > >Using + Slides, cut on 1-2 microns, baking 1/2 hr. Air drying, and this >nail still wants to fall off. > >Any suggestions. Please help, > >Craig > >Sent from my iPhone >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 20 Aug 2015 16:28:36 +0000 From: "Baldwin, Kathy" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] FW: Histo tech position Message-ID: <0456ed5c1ac5425a90b565ae90028d07 at exch02.mhhcc.org> Content-Type: text/plain; charset="us-ascii" From: Baldwin, Kathy Sent: Thursday, August 20, 2015 9:27 AM To: histonet at lists.utsouthwestern.edu Subject: Histo tech position MEMORIAL HOSPITAL AND HEALTH CARE CENTER IS SEEKING: Histology Tech position: Part time 32 hours per week with benefits. Jasper, Indiana 47546 Jasper is a regional center in southwestern Indiana, noted for its heavily German Catholic ancestral roots Jasper has often been called the "Wood Capital of the World", boasting a large number of furniture companies, including Kimball International and Masterbrand Cabinets. Jasper is also home to the Southern Indiana Education Service Center (SIEC), Jasper Engines & Transmissions (largest remanufacturer in the market), and to a satellite campus of Vincennes University. JOB SUMMARY: Responsible for embedding, sectioning and cover slipping paraffin blocks and staining tissues to provide the pathologist with accurately prepared specimens for review and diagnosis. The employee works with specimens from patients of all ages. The employee must reflect mission statement and philosophy of Memorial Hospital and Health Care Center in daily work habits and contacts. QUALIFICATIONS: Education: High school diploma or equivalent is required. Associate degree preferred. HT (ASCP) certification is preferred. Three years' experience as histology assist is acceptable. Training: Six months on the job training required. Experience: Previous histology experience is preferred. New graduates of histology school will be considered. Please contact me, Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Thu, 20 Aug 2015 16:56:54 +0000 From: "Wheelock, Timothy R." To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Formaldehyde sensors and badges Message-ID: <69718C0B0B3C414D9F8E7214AD400CC98C01B089 at PHSX10MB11.partners.org> Content-Type: text/plain; charset="us-ascii" Hi Everyone: We are looking into formaldehyde sensors or badges to monitor our exposure to formaldehyde in our dissection room and our fixed tissue storage room. Does anyone have experience with this that they could share? Thank you so much. Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 10 Date: Thu, 20 Aug 2015 16:58:58 +0000 From: Michael Ann Jones To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] HP IHC Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good Morning, My apologies, I missed the conversations speaking about HP IHC stains previously. Has anyone used the monoclonal antibody for HP? Is it as sensitive and specific as the polyclonal antibody but without the background? We use polyclonal and are experiencing a tremendous amount of background. I've adjusted the stain and can do more, I just wondered if anyone was using monoclonal with better results? Thanks for your help! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 141, Issue 18 ***************************************** From mjones at metropath.com Thu Aug 20 12:50:54 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Thu, 20 Aug 2015 17:50:54 +0000 Subject: [Histonet] Formaldehyde sensors and badges Message-ID: Both Leica and Statlab (probably many labs) sell fume monitor badges for both xylene and formaldehyde. Techs wear badges for work shift and then they are sent to company for analysis reports. Monitoring must be done with job/equipment change, new employees, new process?, etc. Once levels are within acceptable standards, monitoring is optional. We monitor annually, every year always. If levels are above acceptable standards, investigation with documentation must take place and re-testing must be done until levels are within standards. Hope that helps. Michael Ann On 8/20/15, 10:56 AM, "Wheelock, Timothy R. via Histonet" wrote: >Hi Everyone: > >We are looking into formaldehyde sensors or badges to monitor our >exposure to formaldehyde in our dissection room and our fixed tissue >storage room. >Does anyone have experience with this that they could share? >Thank you so much. > >Tim Wheelock >Harvard Brain Tissue Resource Center >McLean Hospital >Belmont, MA > > >The information in this e-mail is intended only for the person to whom it >is >addressed. If you believe this e-mail was sent to you in error and the >e-mail >contains patient information, please contact the Partners Compliance >HelpLine at >http://www.partners.org/complianceline . If the e-mail was sent to you in >error >but does not contain patient information, please contact the sender and >properly >dispose of the e-mail. >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kimberly at animalreferencepathology.com Thu Aug 20 14:05:10 2015 From: Kimberly at animalreferencepathology.com (Kimberly Marshall) Date: Thu, 20 Aug 2015 19:05:10 +0000 Subject: [Histonet] Mouse brain and spinal cord Message-ID: Howdy Histo folks. Quick question. I am working on a mouse brain and spinal cord research project. After cutting at 9 microns and staining routine H&E I had several wash. Today I am sectioning again, and thinking about putting in the oven over night before staining. Will this hurt the tissue?? I am new to animal Histo so learning as I go. I am doing LFB's on these slides too, so will extra dryer time cause problems with that stain? Thanks in advance for any info!!! Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg at 01CF8F87.A0BD4830] From kdonadio at ymail.com Thu Aug 20 19:00:26 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Thu, 20 Aug 2015 17:00:26 -0700 Subject: [Histonet] Formaldehyde sensors and badges In-Reply-To: Message-ID: <1440115226.71675.YahooMailMobile@web121905.mail.ne1.yahoo.com> You can purchase trace gas badges from Mercedes medical. And a few other places. You just wear them or place them for a short term or long term exposure limit then package them back up and send them in. They send you the results. These badges meet CLIA/CAP guidelines. Hope this helps Kim From johnronan at custompathlabsolutions.com Thu Aug 20 21:24:36 2015 From: johnronan at custompathlabsolutions.com (John Ronan) Date: Thu, 20 Aug 2015 22:24:36 -0400 Subject: [Histonet] Equipment to sell Message-ID: On behalf of Gary Casino, Practice Manager at Somerset Urological Associates in Somerville NJ, I want to let you know that the following equipment is for sale. The equipment is from the histo lab which was operational from 2011 until earlier this month. Please email Johnronan at custompathlabsolutions.com if you are interested in any equipment. Thank you, John Ronan GROSS LAB JUNIOR $5,836.80 SHANDON WAX DISPENSER $816.00 BOEKEL WATER BATH $472.20 SHANDON CONVECTION OVEN $445.80 SHANDON CENTRIFUGE IEC CL10 $1,301.40 SHANDON ADAPTERS 4 PK $91.20 0-G26/1 1 HEAD & BUCKET $337.20 2 22 GAL EAGLE FLAMMABLE STORAGE CABINET $1,420.80 17 GAL JUSTRITE FLAMMABLE STORAGE CABINET $90.00 SHANDON Cytospin III $2,280.00 SAKURA DRS 2000 AUTO SLIDE stainer $10,140.00 refrigerator $60.00 freezer $590.40 olympus BX40 MICROSCOPE $2,493.60 Tissuewave 2 microwave processor $7,800.00 From jaylundgren at gmail.com Fri Aug 21 11:16:57 2015 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 21 Aug 2015 11:16:57 -0500 Subject: [Histonet] Freeze Spray Not Sold as Case In-Reply-To: References: Message-ID: There's this cool thing called the Interwebz now? http://www.amazon.com/Multi-Purpose-Freeze-Spray-1-Ea/dp/B0017UIB30#Ask Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Aug 19, 2015 at 8:53 PM, ian bernard via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Fellow Histonetters: I'm looking for a source to purchase Freeze-Spray used > during the frozen procedure. Our current resource sells Freeze Spray in a > case of 6 cans. I would like to purchase as an individual can owing to our > workload rather than a case. > > > > Any references? > > > > Ian Bernard > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From litepath2000 at yahoo.com Fri Aug 21 12:56:56 2015 From: litepath2000 at yahoo.com (NYSHisto) Date: Fri, 21 Aug 2015 17:56:56 +0000 (UTC) Subject: [Histonet] Antibody Suggestions Message-ID: <1678497506.3023853.1440179816359.JavaMail.yahoo@mail.yahoo.com> Does anyone have any suggestions/recommendations on f480 antibodies?? Thanks in advance From patpxs at gmail.com Fri Aug 21 15:33:17 2015 From: patpxs at gmail.com (Patpxs) Date: Fri, 21 Aug 2015 13:33:17 -0700 Subject: [Histonet] Freeze Spray Not Sold as Case Happy Friday In-Reply-To: References: Message-ID: <6DC29599-365C-4219-A664-E3A45C1D1942@gmail.com> I heard everything on the intervention is true, is that true? Paula ;-) Sent from my iPhone > On Aug 21, 2015, at 9:16 AM, Jay Lundgren via Histonet wrote: > > There's this cool thing called the Interwebz now? > > http://www.amazon.com/Multi-Purpose-Freeze-Spray-1-Ea/dp/B0017UIB30#Ask > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > On Wed, Aug 19, 2015 at 8:53 PM, ian bernard via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Fellow Histonetters: I'm looking for a source to purchase Freeze-Spray used >> during the frozen procedure. Our current resource sells Freeze Spray in a >> case of 6 cans. I would like to purchase as an individual can owing to our >> workload rather than a case. >> >> >> >> Any references? >> >> >> >> Ian Bernard >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango at gmail.com Fri Aug 21 16:04:16 2015 From: marktarango at gmail.com (Mark Tarango) Date: Fri, 21 Aug 2015 14:04:16 -0700 Subject: [Histonet] Freeze Spray Not Sold as Case In-Reply-To: References: Message-ID: I used to work in a lab that used compressed air held upside down as freeze spray. Worked the same for me and might be cheaper. On Wed, Aug 19, 2015 at 6:53 PM, ian bernard via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Fellow Histonetters: I'm looking for a source to purchase Freeze-Spray used > during the frozen procedure. Our current resource sells Freeze Spray in a > case of 6 cans. I would like to purchase as an individual can owing to our > workload rather than a case. > > > > Any references? > > > > Ian Bernard > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From twheelock at mclean.harvard.edu Fri Aug 21 16:12:40 2015 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Fri, 21 Aug 2015 21:12:40 +0000 Subject: [Histonet] Thank you Message-ID: <69718C0B0B3C414D9F8E7214AD400CC98C01B1ED@PHSX10MB11.partners.org> Hi Everyone: I want to thank everyone for the advice concerning the chemical sensors and badges. It is very helpful. Have a great weekend. Tim Wheelock The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From riepopp2 at gmail.com Sat Aug 22 18:42:42 2015 From: riepopp2 at gmail.com (Laurie Finch) Date: Sat, 22 Aug 2015 18:42:42 -0500 Subject: [Histonet] F480 antibody Message-ID: Abserotec has a great f4/80 antibody. Laurie From Richard.Cartun at hhchealth.org Sun Aug 23 09:28:46 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Sun, 23 Aug 2015 14:28:46 +0000 Subject: [Histonet] IHC Weekend Coverage Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0379E8@HHCEXCHMB03.hhcsystem.org> How many of you working in "hospital-based" pathology laboratories run IHC on weekends? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From bakevictoria at gmail.com Sun Aug 23 09:54:32 2015 From: bakevictoria at gmail.com (Victoria Baker) Date: Sun, 23 Aug 2015 10:54:32 -0400 Subject: [Histonet] IHC Weekend Coverage In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B0379E8@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B0379E8@HHCEXCHMB03.hhcsystem.org> Message-ID: We have Saturday lab coverage which includes IHC. Case/slides vary between 8-15/25-85. This includes multiplex and ISH. Vikki On Aug 23, 2015 10:49 AM, "Cartun, Richard via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > How many of you working in "hospital-based" pathology laboratories run IHC > on weekends? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bcdukes at lexhealth.org Sun Aug 23 13:16:06 2015 From: bcdukes at lexhealth.org (Blake Taylor) Date: Sun, 23 Aug 2015 18:16:06 +0000 Subject: [Histonet] IHC weekend coverage Message-ID: We have Saturday lab coverage which consists of a single histotech and only inpatient OR samples are cut. We do not perform IHC or specials on the weekend. Blake Taylor -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Sunday, August 23, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 141, Issue 21 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. F480 antibody (Laurie Finch) 2. IHC Weekend Coverage (Cartun, Richard) 3. Re: IHC Weekend Coverage (Victoria Baker) ---------------------------------------------------------------------- Message: 1 Date: Sat, 22 Aug 2015 18:42:42 -0500 From: Laurie Finch To: litepath2000 at yahoo.com, histonet at lists.utsouthwestern.edu Subject: [Histonet] F480 antibody Message-ID: Content-Type: text/plain; charset=UTF-8 Abserotec has a great f4/80 antibody. Laurie ------------------------------ Message: 2 Date: Sun, 23 Aug 2015 14:28:46 +0000 From: "Cartun, Richard" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] IHC Weekend Coverage Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0379E8 at HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" How many of you working in "hospital-based" pathology laboratories run IHC on weekends? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 3 Date: Sun, 23 Aug 2015 10:54:32 -0400 From: Victoria Baker To: "Cartun, Richard" Cc: histonet netserver Subject: Re: [Histonet] IHC Weekend Coverage Message-ID: Content-Type: text/plain; charset=UTF-8 We have Saturday lab coverage which includes IHC. Case/slides vary between 8-15/25-85. This includes multiplex and ISH. Vikki On Aug 23, 2015 10:49 AM, "Cartun, Richard via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > How many of you working in "hospital-based" pathology laboratories run > IHC on weekends? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution > is prohibited. If you are not the intended recipient, or an employee > or agent responsible for delivering the message to the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 141, Issue 21 ***************************************** PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From carl.hobbs at kcl.ac.uk Sun Aug 23 13:58:26 2015 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 23 Aug 2015 18:58:26 +0000 Subject: [Histonet] F480 antibody Message-ID: Doesn't work in Pwax sections...for me. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From kdonadio at ymail.com Sun Aug 23 15:48:22 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Sun, 23 Aug 2015 13:48:22 -0700 Subject: [Histonet] IHC Weekend Coverage In-Reply-To: Message-ID: <1440362902.40277.YahooMailMobile@web121903.mail.ne1.yahoo.com> I never did on a routine basis. The only time was rare urgent cases or cases that needed done due to someone off or equipment problems. Kim D From Maxim_71 at mail.ru Sun Aug 23 15:57:33 2015 From: Maxim_71 at mail.ru (Maxim Peshkov) Date: Sun, 23 Aug 2015 23:57:33 +0300 Subject: [Histonet] Search contact Message-ID: <1543788543.20150823235733@mail.ru> I am searching the contact with Janet Minshew from Texas. Janet, please, if it possible, e-mail for me directly: Maxim_71 at mail.ru -- Maxim Peshkov, Russia, Taganrog. From Timothy.Morken at ucsf.edu Mon Aug 24 10:06:03 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 24 Aug 2015 15:06:03 +0000 Subject: [Histonet] IHC Weekend Coverage In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B0379E8@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B0379E8@HHCEXCHMB03.hhcsystem.org> Message-ID: <761E2B5697F795489C8710BCC72141FF6030DE9F@ex07.net.ucsf.edu> Rich, We don't offer any IHC on weekends or holidays. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Sunday, August 23, 2015 7:29 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC Weekend Coverage How many of you working in "hospital-based" pathology laboratories run IHC on weekends? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Mon Aug 24 10:08:54 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 24 Aug 2015 15:08:54 +0000 Subject: [Histonet] Weekend IHC In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F802951D@HRHEX02-HOS.holyredeemer.local> Med size hosp lab - not here Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. IHC Weekend Coverage (Cartun, Richard) 3. Re: IHC Weekend Coverage (Victoria Baker) Message: 2 Date: Sun, 23 Aug 2015 14:28:46 +0000 From: "Cartun, Richard" Subject: [Histonet] IHC Weekend Coverage How many of you working in "hospital-based" pathology laboratories run IHC on weekends? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax From nlinke at sbch.org Mon Aug 24 12:05:16 2015 From: nlinke at sbch.org (Noelle Linke) Date: Mon, 24 Aug 2015 17:05:16 +0000 Subject: [Histonet] IHC Weekend Coverage In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B0379E8@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B0379E8@HHCEXCHMB03.hhcsystem.org> Message-ID: Nope Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Sunday, August 23, 2015 7:29 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC Weekend Coverage How many of you working in "hospital-based" pathology laboratories run IHC on weekends? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CHS Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health System, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ From JRobinson at pathology-associates.com Mon Aug 24 12:45:49 2015 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Mon, 24 Aug 2015 17:45:49 +0000 Subject: [Histonet] IHC Weekend Coverage In-Reply-To: <761E2B5697F795489C8710BCC72141FF6030DE9F@ex07.net.ucsf.edu> References: <9215BD4B0BA1B44D962A71C758B68D2E6B0379E8@HHCEXCHMB03.hhcsystem.org> <761E2B5697F795489C8710BCC72141FF6030DE9F@ex07.net.ucsf.edu> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D9F561@PAEXCH1.PathologyAssociates.local> We run IHC overnight on Friday night and IHC for the Saturday pathologist (on call pathologist on a rotating basis with 17 total pathologists). We have several pathologists who use Saturday as a catch up day and will come in to read their pending IHC stains. Jeff Robinson, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, August 24, 2015 8:06 AM To: Cartun, Richard Cc: Histonet Subject: Re: [Histonet] IHC Weekend Coverage Rich, We don't offer any IHC on weekends or holidays. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Sunday, August 23, 2015 7:29 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC Weekend Coverage How many of you working in "hospital-based" pathology laboratories run IHC on weekends? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From Michael.LaFriniere at ccplab.com Mon Aug 24 13:09:18 2015 From: Michael.LaFriniere at ccplab.com (Michael LaFriniere) Date: Mon, 24 Aug 2015 18:09:18 +0000 Subject: [Histonet] Mouse brain and spinal cord In-Reply-To: References: Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D297468D2@AHCMSASEXCH02.my.ahc.local> In my experience Shouldn't hurt at 70-75 degrees c in oven over night Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041?Bournefield Way, Suite?A . Silver Spring, MD 20904?? P:?240.471.3427?. F:?240.471.3401 . Cell 410-940-8844 michael.lafriniere at ccplab.com -----Original Message----- From: Kimberly Marshall via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 20, 2015 3:05 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Mouse brain and spinal cord Howdy Histo folks. Quick question. I am working on a mouse brain and spinal cord research project. After cutting at 9 microns and staining routine H&E I had several wash. Today I am sectioning again, and thinking about putting in the oven over night before staining. Will this hurt the tissue?? I am new to animal Histo so learning as I go. I am doing LFB's on these slides too, so will extra dryer time cause problems with that stain? Thanks in advance for any info!!! Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg at 01CF8F87.A0BD4830] _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From craigak12 at gmail.com Mon Aug 24 15:43:46 2015 From: craigak12 at gmail.com (Jb) Date: Mon, 24 Aug 2015 13:43:46 -0700 Subject: [Histonet] Tissue disposal procedure: Message-ID: Does anyone have a tissue disposal procedure that they would be willing to share? I am looking to perfect the one we have and would love to see how others are writing this up for staff. Sincerely, Craig Sent from my iPhone From rob at foliobio.com Tue Aug 25 06:53:24 2015 From: rob at foliobio.com (Rob Day) Date: Tue, 25 Aug 2015 07:53:24 -0400 Subject: [Histonet] disposal Message-ID: <986F30DB-29DF-4462-A1A2-711B10087069@foliobio.com> You might consider donating CAP graduated or unwanted samples to a non-profit or commercial biobank. Ethically sourced biospecimens are always in demand for research. In many cases the biobank will take care of the cost and logistics of pickup and transportation. Check with your IRB first and, of course, be sure you understand all issues related to donor consent and / or exemption as per US 45CFR46. Rob. > Does anyone have a tissue disposal procedure that they would be willing to share? I am looking to perfect the one we have and would love to see how others are writing this up for staff. > > Sincerely, > > Craig From beth.villarreal at novartis.com Tue Aug 25 08:45:45 2015 From: beth.villarreal at novartis.com (Villarreal, Beth) Date: Tue, 25 Aug 2015 13:45:45 +0000 Subject: [Histonet] UMFix for paraffin blocks on standard tissue processor Message-ID: <0a8980ef03604e02a7fc6395c1a084ab@DM2PR62MB032.023d.mgd.msft.net> Hi all, I'm interested in testing out UMFix, but want to process on our standard tissue processor (not microwave, not the Sakura Xpress system). Has anyone done this? Would you be willing to share your protocol? Thanks in advance! Beth From twheelock at mclean.harvard.edu Tue Aug 25 09:06:36 2015 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Tue, 25 Aug 2015 14:06:36 +0000 Subject: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens Message-ID: <69718C0B0B3C414D9F8E7214AD400CC98C01C429@PHSX10MB11.partners.org> Hi Everyone: It appears that for security and privacy reasons, the NIH wants us to change from an internal specimen tracking system that employees sequential numbers(8634, 8635, 8636 etc.) to a system that uses randomly generated anonymizing number (20487, 71936, 88011 etc.) It seems to me that this invites mistakes and mixing up of cases. (Humans seem to deal better with sequential numbers). This would include everything, from the buckets with formaldehyde in which half brains are fixed, to wax blocks, to slides, to block and slide files, to the images that I take on each case. Does anyone have experience using computer generated random anonymizing tracking numbers in their pathology or tissue banking departments? What system of checks do you employee to avoid mistakes and make the work go smoothly? Perhaps this system will work fine, once we are used to it. Thank you very much for any feedback. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From esulkosky at gmail.com Tue Aug 25 09:42:48 2015 From: esulkosky at gmail.com (Eric Sulkosky) Date: Tue, 25 Aug 2015 10:42:48 -0400 Subject: [Histonet] Histology Opening at DermPath Diagnostics-Pittsburgh, PA Message-ID: Good morning Histonet, DermPath Diagnostics - A Division of Quest Diagnostics is currently recruiting for a full time Histotechnician II in our Pittsburgh location. Great opportunity for anyone seeking a challenging career in Dermpath. Position comes with full benefits package to include health, vision, eye, dental, 401K and stock options. If interested please visit www.QuestDiagnostics.com and search for Job ID 3747609 to apply. Or you can email a copy of your resume to esulkosky at ameripath.com We look forward to hearing from you soon. From rjbuesa at yahoo.com Tue Aug 25 09:48:29 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 25 Aug 2015 14:48:29 +0000 (UTC) Subject: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC98C01C429@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC98C01C429@PHSX10MB11.partners.org> Message-ID: <245320788.283915.1440514109209.JavaMail.yahoo@mail.yahoo.com> As I see it, the only way this system may work is if you have a "code" to determine what those random numbers mean and which samples they belong to which, in itself, will "defeat" the randomization objective.Otherwise this will be "chaos" in any lab, and the bigger the greater the chaos.To me it is the most stupid, although "anonymously correct" proposition that I am sure was designed by a "number pusher" seated in his/her desk and with little to do at the moment and with no actual idea of how a pathology lab works and what would imply mixing up specimens or being unable to be SURE which sample belongs to whom.I would strongly oppose it!Ren?? On Tuesday, August 25, 2015 10:18 AM, "Wheelock, Timothy R. via Histonet" wrote: Hi Everyone: It appears that for security and privacy reasons, the NIH wants us to change from an internal specimen tracking system that employees sequential numbers(8634, 8635, 8636 etc.) to a system that uses randomly generated anonymizing number (20487, 71936, 88011 etc.) It seems to me that this invites mistakes and mixing up of cases. (Humans seem to deal better with sequential numbers). This would include everything, from the buckets with formaldehyde in which half brains are fixed, to wax blocks, to slides, to block and slide files, to the images that I take on each case. ? ? ? ? ? ? ? ? Does anyone have experience using? computer generated random anonymizing tracking numbers in their pathology or tissue banking departments? What system of checks do you employee to? avoid mistakes and make the work go smoothly? Perhaps this system will work fine, once we are used to it. ? ? ? ? ? ? ? ? Thank you very much for any feedback. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From duraine at bcm.edu Tue Aug 25 09:58:47 2015 From: duraine at bcm.edu (Duraine, Lita) Date: Tue, 25 Aug 2015 14:58:47 +0000 Subject: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens Message-ID: I agree with Rene, and everyone else that using random generated tracking numbers are a really, really, big mistake. We do EM here and it would be a nightmare if we used random numbers. There are ways to use tracking numbers where the ID of an individual is not compromised. Perhaps NIH should have considered the outcome first. Oh well, I guess they should get ready to apologize in a few months. Lita Duraine Certified EM Specialist From Michael.LaFriniere at ccplab.com Tue Aug 25 11:00:07 2015 From: Michael.LaFriniere at ccplab.com (Michael LaFriniere) Date: Tue, 25 Aug 2015 16:00:07 +0000 Subject: [Histonet] FW: Using randomly generated anonymizing numbers for internal tracting of specimens References: <69718C0B0B3C414D9F8E7214AD400CC98C01C429@PHSX10MB11.partners.org> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29746C0F@AHCMSASEXCH02.my.ahc.local> -----Original Message----- From: Michael LaFriniere Sent: Tuesday, August 25, 2015 10:31 AM To: 'Wheelock, Timothy R.' Subject: RE: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens Since we also perform types of Research in our laboratory for several companies, Is there a particular reason why NIH is asking for this? Unless there is a sound reasoning for such a request, I agree, this may just be inviting problems Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041?Bournefield Way, Suite?A . Silver Spring, MD 20904 P:?240.471.3427?. F:?240.471.3401 . Cell 410-940-8844 michael.lafriniere at ccplab.com -----Original Message----- From: Wheelock, Timothy R. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 25, 2015 10:07 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens Hi Everyone: It appears that for security and privacy reasons, the NIH wants us to change from an internal specimen tracking system that employees sequential numbers(8634, 8635, 8636 etc.) to a system that uses randomly generated anonymizing number (20487, 71936, 88011 etc.) It seems to me that this invites mistakes and mixing up of cases. (Humans seem to deal better with sequential numbers). This would include everything, from the buckets with formaldehyde in which half brains are fixed, to wax blocks, to slides, to block and slide files, to the images that I take on each case. Does anyone have experience using computer generated random anonymizing tracking numbers in their pathology or tissue banking departments? What system of checks do you employee to avoid mistakes and make the work go smoothly? Perhaps this system will work fine, once we are used to it. Thank you very much for any feedback. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Tue Aug 25 11:44:57 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 25 Aug 2015 16:44:57 +0000 Subject: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC98C01C429@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC98C01C429@PHSX10MB11.partners.org> Message-ID: <761E2B5697F795489C8710BCC72141FF6030E1DA@ex07.net.ucsf.edu> Interesting. I can see the point if they want total opacity as to person ID. Is only the number supposed to appear on the label? How about a second identifier? It would not cause mistakes in labeling (because the labels come from the system) as long as you are following one-piece workflow. But doing so will make it necessary to scan a barcode (or OCR to read the number) to open any case for anything. Necessary because typos on manual entering will be a problem (unless you trust people to ALWAYS check a second identifier. I don't!). It is not really so bad as long as you have easy access to a computer at all times in order to look up the case, including all slide, block and tissue storage. And, how do you store materials? We do it now essentially by date (sequential number). How do you store and retrieve a random number? Randomly? If you had a robotic warehouse it could be done. Do you? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Wheelock, Timothy R. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 25, 2015 7:07 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens Hi Everyone: It appears that for security and privacy reasons, the NIH wants us to change from an internal specimen tracking system that employees sequential numbers(8634, 8635, 8636 etc.) to a system that uses randomly generated anonymizing number (20487, 71936, 88011 etc.) It seems to me that this invites mistakes and mixing up of cases. (Humans seem to deal better with sequential numbers). This would include everything, from the buckets with formaldehyde in which half brains are fixed, to wax blocks, to slides, to block and slide files, to the images that I take on each case. Does anyone have experience using computer generated random anonymizing tracking numbers in their pathology or tissue banking departments? What system of checks do you employee to avoid mistakes and make the work go smoothly? Perhaps this system will work fine, once we are used to it. Thank you very much for any feedback. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw at personifysearch.com Tue Aug 25 12:06:16 2015 From: mw at personifysearch.com (Matt Ward) Date: Tue, 25 Aug 2015 13:06:16 -0400 Subject: [Histonet] Histology Field Applications Specialist - Northeast Message-ID: <45df1b939261d47547758a41decad2d2@mail.gmail.com> Hello Histonet! I hope everyone is enjoying the summer! We have recently had a top client open a new field based histology specialist opportunity to support their customers in the Northeast. The ideal candidate will have a 4 year degree and a strong histology background. The position is field based and the ideal location would be in the Boston area. Please contact me directly to learn more at ? mw at personifysearch.com. Thanks! Matt Matt Ward *Program Manager ? RPO* *Personify * 5020 Weston Parkway Suite 315 Cary NC 27513 Direct Line: (919) 459-3654 Toll Free: (800) 875-6188 ext. 103 www.personifysearch.com https://www.linkedin.com/in/mattwardpersonify [image: https://docs.google.com/uc?export=download&id=0B7dv3zi00-kodmd5T1pwSWFtSVE&revid=0B7dv3zi00-koZXMxaHA0WHJWa1pYUElUUGxLWGtQSnNaNXV3PQ] From bliven.laura at marshfieldclinic.org Tue Aug 25 12:44:08 2015 From: bliven.laura at marshfieldclinic.org (Bliven, Laura M) Date: Tue, 25 Aug 2015 17:44:08 +0000 Subject: [Histonet] Labeling Slides Message-ID: Does anyone label histology slides (H&E's, special stains, or IHC slides) without writing on the slide itself? If you have a control section on a slide and are placing the patient tissue also on the slide, would you ever run the control slide through a slide printer? Thanks Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From kdonadio at ymail.com Tue Aug 25 13:07:42 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Tue, 25 Aug 2015 18:07:42 +0000 (UTC) Subject: [Histonet] FW: Using randomly generated anonymizing numbers for internal tracting of specimens In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D29746C0F@AHCMSASEXCH02.my.ahc.local> References: <4A2A16B9707CE04E9CB6C82DC18C1D29746C0F@AHCMSASEXCH02.my.ahc.local> Message-ID: <1169744120.409510.1440526062779.JavaMail.yahoo@mail.yahoo.com> I agree, inviting problems. Having the numbers in consecutive order is helpful for numerous reasons. ?Also, I can not imagine how having one case as 85310 and the next case as 14275 be anymore of a security or privacy improvement as 1435, 1436. They still get a number. ?Horrible idea in my opinion.?Kim D From: Michael LaFriniere via Histonet To: "histonet at lists.utsouthwestern.edu" Sent: Tuesday, August 25, 2015 11:00 AM Subject: [Histonet] FW: Using randomly generated anonymizing numbers for internal tracting of specimens -----Original Message----- From: Michael LaFriniere Sent: Tuesday, August 25, 2015 10:31 AM To: 'Wheelock, Timothy R.' Subject: RE: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens Since we also perform types of Research in our laboratory for several companies, Is there a particular reason why NIH is asking for this? Unless there is a sound reasoning for such a request, I agree, this may just be inviting problems Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041?Bournefield Way, Suite?A . Silver Spring, MD 20904 P:?240.471.3427?. F:?240.471.3401 . Cell 410-940-8844 michael.lafriniere at ccplab.com -----Original Message----- From: Wheelock, Timothy R. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 25, 2015 10:07 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Using randomly generated anonymizing numbers for internal tracting of specimens Hi Everyone: It appears that for security and privacy reasons, the NIH wants us to change from an internal specimen tracking system that employees sequential numbers(8634, 8635, 8636 etc.) to a system that uses randomly generated anonymizing number (20487, 71936, 88011 etc.) It seems to me that this invites mistakes and mixing up of cases. (Humans seem to deal better with sequential numbers). This would include everything, from the buckets with formaldehyde in which half brains are fixed, to wax blocks, to slides, to block and slide files, to the images that I take on each case. ? ? ? ? ? ? ? ? Does anyone have experience using? computer generated random anonymizing tracking numbers in their pathology or tissue banking departments? What system of checks do you employee to? avoid mistakes and make the work go smoothly? Perhaps this system will work fine, once we are used to it. ? ? ? ? ? ? ? ? Thank you very much for any feedback. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Tue Aug 25 13:04:07 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 25 Aug 2015 18:04:07 +0000 Subject: [Histonet] Labeling Slides In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6030E293@ex07.net.ucsf.edu> Laura, We don't hand-write anything on case slides before applying a printed label, if that is what you mean. Hand-written labels are the most error-prone method of labeling. We use a TBS slide etcher to batch-print on-slide labels for our on-slide control TMA's and single-tissue control slides. At the microtome we print a label for the case which is applied to the slide when the case tissue is cut and mounted on the slide with control tma/tissue. That is one advantage of using applied labels for cases slides rather than a direct-printed slide label. We have run slides through the slide etcher with tissue on them, and had pretty good, though not perfect, results. That is, sometime the tissue section would be damaged. It is not really best practice. It works ok for control tissue since there are a lot of slides , but I would not do it for patient tissue. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Bliven, Laura M via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 25, 2015 10:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Labeling Slides Does anyone label histology slides (H&E's, special stains, or IHC slides) without writing on the slide itself? If you have a control section on a slide and are placing the patient tissue also on the slide, would you ever run the control slide through a slide printer? Thanks Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock at mclean.harvard.edu Tue Aug 25 15:00:45 2015 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Tue, 25 Aug 2015 20:00:45 +0000 Subject: [Histonet] Anonymizing numbering system Message-ID: <69718C0B0B3C414D9F8E7214AD400CC98C01C53C@PHSX10MB11.partners.org> Hi Everyone: Thank you for all your responses and thoughts on this issue. I should clarify that this new system may actually not be coming from the NIH itself, but perhaps from departments in one of our parent organizations. We have no written sources for this sort of approach. They may be understandably concerned about separating personal identifying information from clinical/pathological diagnostic information, so as to ensure confidentiality, especially in this day and age of increasingly vulnerable personal information. Assigning a randomized anonymizing number tracking system may help in this regard. I have been assured that a "code", that is a way of matching the anonymizing number to a sequential system would be in place. So there would always be a way of "getting our bearings" at any point. My concern was mistakes and mixing up of cases. I still would prefer a sequential system, but at this point, we don't really have an option. Plus, maybe it is just that I am used to a sequential system. I think it may be difficult to get use to at first, but if we take it carefully, and everyone cross-checks each other, it should be do-able. We shall see. Thanks again. Tim The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rjbuesa at yahoo.com Tue Aug 25 15:22:42 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 25 Aug 2015 20:22:42 +0000 (UTC) Subject: [Histonet] Anonymizing numbering system In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC98C01C53C@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC98C01C53C@PHSX10MB11.partners.org> Message-ID: <473311095.520206.1440534162755.JavaMail.yahoo@mail.yahoo.com> Good! So probably when you really mess-up somebody's diagnose and have to pay several million of dollars in a law suit, your "higher echelons" will realize how stupid is the idea.Besides if you give a randomized number to a specimen and later use a code to?"decipher" the?randomized number, where is the "anonymity" advantage? Is it not the same as having a sequential number without the patient's name, and the list of cases with patient's names and their sequential numbers? I really cannot comprehend the rationale and I am also sure that whomever came up?with this "brilliant" idea makes much more money that you and never has worked in a pathology lab. I pity your position of having to accept a stupid procedure and being unable to hold your ground because of fear of losing your job.It is infuriating realize how stupid people?"claim the?ladder of power" Ren? On Tuesday, August 25, 2015 4:10 PM, "Wheelock, Timothy R. via Histonet" wrote: Hi Everyone: Thank you for all your responses and thoughts on this issue. I should clarify that this new system may actually not be coming from the NIH itself, but perhaps from departments in one of our parent organizations. We have no written sources for this sort of approach. They may be understandably concerned about separating personal identifying information from clinical/pathological diagnostic information, so as to ensure confidentiality, especially in this day and age of increasingly vulnerable personal information. Assigning a randomized anonymizing number tracking system may help in this regard.? I have been assured that a "code", that is a way of matching the anonymizing number to a sequential system would be in place. So there would always be a way of "getting our bearings" at any point. My concern was mistakes and mixing up of cases. I still would prefer a sequential system, but at this point, we don't really have an option.? Plus, maybe it is just that I am used to a sequential system. I think it may be difficult to get use to at first, but if we take it carefully, and everyone cross-checks each other, it should be do-able. We shall see. Thanks again. Tim The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdonadio at ymail.com Tue Aug 25 16:53:50 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Tue, 25 Aug 2015 14:53:50 -0700 Subject: [Histonet] Anonymizing numbering system In-Reply-To: <473311095.520206.1440534162755.JavaMail.yahoo@mail.yahoo.com> Message-ID: <1440539630.73900.YahooMailMobile@web121906.mail.ne1.yahoo.com> Sounds like a nightmare. This is going to be time consuming at a time when we are all supposed to be lean and quick. I hope this doesn't roll out as a regulation. If so we need to fight it tooth and nail. Just imagine trying to find a block or a slide all filed with random assigned numbers. And that's just a small problem. While computers can easily push out complex random numbers and reconize them humans dont. You have my greatest sympathy. Good luck Kim From patpxs at gmail.com Tue Aug 25 17:43:06 2015 From: patpxs at gmail.com (Pele Conqueror) Date: Tue, 25 Aug 2015 15:43:06 -0700 Subject: [Histonet] Recut Rates? Message-ID: Good Afternoon Netters, What do you all consider a good recut rate? I'm going for an average group rate, not individual. Perhaps for the week or month, not daily. How do you track it? Thanks in advance. Toodles, Paula :-) From rjbuesa at yahoo.com Wed Aug 26 07:17:03 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 26 Aug 2015 12:17:03 +0000 (UTC) Subject: [Histonet] Recut Rates? In-Reply-To: References: Message-ID: <1146659220.883505.1440591423671.JavaMail.yahoo@mail.yahoo.com> Please go to: http://www.histosearch.com/rene/html?to find answer to your query. Ren? On Tuesday, August 25, 2015 6:48 PM, Pele Conqueror via Histonet wrote: Good Afternoon Netters, What do you all consider a good recut rate? I'm going for an average group rate, not individual.? Perhaps for the week or month, not daily. How do you track it? Thanks in advance. Toodles, Paula? :-) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis at che-east.org Wed Aug 26 08:01:09 2015 From: CDavis at che-east.org (Davis, Cassie) Date: Wed, 26 Aug 2015 09:01:09 -0400 Subject: [Histonet] IHC Weekend Coverage Message-ID: No IHC/HC/H&E weekend coverage. If there is a Stat the supervisor comes in. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ekaterina.vinnik at neuro.fchampalimaud.org Wed Aug 26 10:10:21 2015 From: ekaterina.vinnik at neuro.fchampalimaud.org (Ekaterina Vinnik) Date: Wed, 26 Aug 2015 16:10:21 +0100 Subject: [Histonet] rodent brain: how to avoid air bubbles in the ventricles after perfusion? Message-ID: Dear all, I'm new to this list - and was really happy to find it. Does anybody have any advice on how to do rat brain extraction in such a way that the air would not get into the ventricles? We are doing MRI on the ex vivo rat brains, and the air which gets in causes nasty artifacts. I am looking for consistent way to avoid the bubbles, cause . The way we are extracting the brain now is to isolate the head, peel the skull from the top and from the sides, crush the nasal bone, expose the bulbs, remove dura, cut the nerves, lift the brain.. I was never concerned with this problem until I started doing MRI - and here it turns out to be really important. I was wondering if I should just leave the dura in place during extraction, for example, would that work? Thank you in advance! Katya -- Ekaterina Vinnik, MD, PhD Behavioural neuroscience lab, Champalimaud Neuroscience Programme, Lisbon, Portugal +351918951392 lulswinnik at gmail.com From KSimeone at leavittmgt.com Wed Aug 26 11:01:18 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 26 Aug 2015 16:01:18 +0000 Subject: [Histonet] FULLTIME DAY SHIFT (first shift) POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DCE8A@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Beach, Florida dermatology laboratory. This is a permanent full time, DAYTIME SHIFT (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html *full time position Mon-Fri 8a-5:30p (start time may vary) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Michael.Baker at cchmc.org Wed Aug 26 12:07:22 2015 From: Michael.Baker at cchmc.org (Baker, Michael) Date: Wed, 26 Aug 2015 17:07:22 +0000 Subject: [Histonet] rodent bubble brains In-Reply-To: References: Message-ID: Hello, my simple mind is thinking: can you scan before extracting the brain? or, can you extract the brain under water? Michael Baker, M.D. (CCHMC Pathology) > Message: 11 > Date: Wed, 26 Aug 2015 16:10:21 +0100 > From: Ekaterina Vinnik > Subject: rodent brain: how to avoid air bubbles in the ventricles after perfusion? > > Dear all, > I'm new to this list - and was really happy to find it. Does anybody have > any advice on how to do rat brain extraction in such a way that the air > would not get into the ventricles? We are doing MRI on the ex vivo rat > brains, and the air which gets in causes nasty artifacts. I am looking for > consistent way to avoid the bubbles, cause . > The way we are extracting the brain now is to isolate the head, peel the > skull from the top and from the sides, crush the nasal bone, expose the > bulbs, remove dura, cut the nerves, lift the brain.. I was never concerned > with this problem until I started doing MRI - and here it turns out to be > really important. > > I was wondering if I should just leave the dura in place during extraction, > for example, would that work? > > Thank you in advance! > Katya > > -- > Ekaterina Vinnik, MD, PhD > Behavioural neuroscience lab, > Champalimaud Neuroscience Programme, > Lisbon, > Portugal > > +351918951392 > lulswinnik at gmail.com ---- Michael Baker, M.D. CCHMC Pathology T (513) 636-4261 F (513) 636-3924 From HMLaudon at gundersenhealth.org Wed Aug 26 12:36:26 2015 From: HMLaudon at gundersenhealth.org (Laudon, Heather M) Date: Wed, 26 Aug 2015 17:36:26 +0000 Subject: [Histonet] Fulltime Opening LaCrosse, WI Message-ID: Hi All, Gundersen Health System located in LaCrosse, Wisconsin has a fulltime Histology Technician position available. Go to http://www.gundersenhealth.org/careers for position description and information on how to apply! Heather Laudon, BS, HTL (ASCP)cm Histology Technician Surgical Pathology Laboratory Gundersen Health System 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 hmlaudon at gundersenhealth.org From kdonadio at ymail.com Wed Aug 26 13:20:15 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Wed, 26 Aug 2015 18:20:15 +0000 (UTC) Subject: [Histonet] rodent brain: how to avoid air bubbles in the ventricles after perfusion? In-Reply-To: References: Message-ID: <935316078.1088256.1440613215257.JavaMail.yahoo@mail.yahoo.com> Maybe you could try clamping off the central canal when you sever the spinal chord to keep the csf from draining out? ?This is just my wild guess. ?Kim D From: Ekaterina Vinnik via Histonet To: histonet at lists.utsouthwestern.edu Sent: Wednesday, August 26, 2015 10:10 AM Subject: [Histonet] rodent brain: how to avoid air bubbles in the ventricles after perfusion? Dear all, I'm new to this list - and was really happy to find it. Does anybody have any advice on how to do rat brain extraction in such a way that the air would not get into the ventricles? We are doing MRI on the ex vivo rat brains, and the air which gets in causes nasty artifacts. I am looking for consistent way to avoid the bubbles, cause . The way we are extracting the brain now is to isolate the head, peel the skull from the top and from the sides, crush the nasal bone, expose the bulbs, remove dura, cut the nerves, lift the brain..? I was never concerned with this problem until I started doing MRI - and here it turns out to be really important. I was wondering if I should just leave the dura in place during extraction, for example, would that work? Thank you in advance! Katya -- Ekaterina Vinnik, MD, PhD Behavioural neuroscience lab, Champalimaud Neuroscience Programme, Lisbon, Portugal +351918951392 lulswinnik at gmail.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Wed Aug 26 14:25:03 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 26 Aug 2015 19:25:03 +0000 Subject: [Histonet] Labeling Slides In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F8029F3C@HRHEX02-HOS.holyredeemer.local> Hi Laura - We print all of our slides using the Leica printers. Even batch cut control slides are pre-printed. The only time we write on the slides is if we cut patient tissue applied to the control slide for IHC staining. Then we will add the Block ID and patient name by hand to the control slide, but a barcode label with all patient information is applied to the slide for processing. I've not had good results sending stained or unstained slides though the printer. I hope this helps. Regards, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. Labeling Slides (Bliven, Laura M) Message: 2 Date: Tue, 25 Aug 2015 17:44:08 +0000 From: "Bliven, Laura M" Subject: [Histonet] Labeling Slides Does anyone label histology slides (H&E's, special stains, or IHC slides) without writing on the slide itself? If you have a control section on a slide and are placing the patient tissue also on the slide, would you ever run the control slide through a slide printer? Thanks Laura From POWELL_SA at mercer.edu Wed Aug 26 16:06:03 2015 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Wed, 26 Aug 2015 17:06:03 -0400 Subject: [Histonet] Mollusk, gastropods Message-ID: <9BF995BC0E47744E9673A41486E24EE25C03F65F0A@MERCERMAIL.MercerU.local> Hello to the wonderful world of histonet. I have been asked to section mollusks/gastropods for research. Let me just say I have no experience in this area, but always willing to expand my knowledge and expertise in histology. The source of the specimens say that the critters were dead before putting them in formalin, they died for reasons unknown and have no normal ones to compare to the diseased ones. I would like to hear from anyone who has sectioned snails and I would like to see photos of sections on the mollusks that were viable before going into the formalin. The ones I cut sectioned well but not sure what they are supposed to look like when healthy and fixed properly. We have googled and looked in books without success. So it was time to "Ask Histonet". Please respond to me privately if you have experience with sectioning mollusks and are willing to share photos and information. powell_sa at mercer.edu Thanks in advance. Shirley Powell From Lacie.Algeo at providence.org Thu Aug 27 12:25:31 2015 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Thu, 27 Aug 2015 17:25:31 +0000 Subject: [Histonet] buildup in grossing sinks Message-ID: <24C4B3C167E5694887AB594C7602CE3A03BFFF8A@WN35104.or.providence.org> Hi All, Does anyone have a recommendation for getting the 'chemical' buildup out of the grossing sinks? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From CDavis at che-east.org Thu Aug 27 12:27:26 2015 From: CDavis at che-east.org (Davis, Cassie) Date: Thu, 27 Aug 2015 13:27:26 -0400 Subject: [Histonet] frozen section- workload and billing Message-ID: Hi Histofolks, I need to pick yours brains...we are in the middle of building a workable computer system for our lab we have run into a hiccup when it come to frozen sections. As a tech I know there is actual hands on, stop what you are doing, do this now work involved. My understanding from a billing perspective it is not "billable workload" but an "interdepartmental consultation between surgery and pathology". The problem is how to build the system so we get labels for our frozen section slides that does not interfere with the "billable workload" that is. I was thinking maybe it should be built in the system the same way a control slide is, does anybody have any suggestions? Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tgenade at gmail.com Thu Aug 27 14:18:42 2015 From: tgenade at gmail.com (Tyrone Genade) Date: Thu, 27 Aug 2015 14:18:42 -0500 Subject: [Histonet] positive control tissues needed: Parkinson's Message-ID: Hello, I am looking for positive control tissues of Parkinson's Disease. I have contacted National Disease Research Interchange but they can only supply me with "whole brains" and hemispheres which is way more than I need and don't want to waste tissue. I am looking for samples of the following: substantia nigra Dorsal Motor Nucleus CN ten (vagus) any tissue that will show alpha-synuclein Lewy neurites Is there anyone out there who has a tissue block spare of the above that they could donate towards my research? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From Karen.Heckford at DignityHealth.org Fri Aug 28 06:58:11 2015 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Fri, 28 Aug 2015 04:58:11 -0700 Subject: [Histonet] Temperature for slides Message-ID: Okay I just got a weird request for monitoring the room temperature where I have my slides for that I use for Histology. We are talking blank slides. I have never heard of this before. Who else does this??? Apparently JCAHO wants this. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From akemiat3377 at gmail.com Fri Aug 28 08:06:26 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Fri, 28 Aug 2015 06:06:26 -0700 Subject: [Histonet] Temperature for slides In-Reply-To: References: Message-ID: We monitor room temp and record it as per CAP, and CLIA. Are you sure the request wasn?t for monitoring room temp because of paraffin blocks, not slides? The concern is for paraffin blocks melting, due to excessive heat. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aallison at montereygi.com Tele: (831) 375-3577 X117 > On Aug 28, 2015, at 4:58 AM, Heckford, Karen - SMMC-SF via Histonet wrote: > > Okay I just got a weird request for monitoring the room temperature where I have my slides for that I use for Histology. We are talking blank slides. I have never heard of this before. Who else does this??? Apparently JCAHO wants this. > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas at biopath.org Fri Aug 28 09:26:42 2015 From: plucas at biopath.org (Paula) Date: Fri, 28 Aug 2015 07:26:42 -0700 Subject: [Histonet] Per Diem Histotech Message-ID: <001601d0e19d$90b88030$b2298090$@biopath.org> Looking for a per diem histotech that can embed and cut our cases during requested vacations throughout the year. We start our day between 430am-7am, so any time to start in this time period works for us. Please send resume to plucas at biopath.org or fax to 714-755-2984 Thank you, Paula Fountain Valley, California Bio-Path Medical Group From jill.cox at cox.net Fri Aug 28 10:02:19 2015 From: jill.cox at cox.net (jill.cox at cox.net) Date: Fri, 28 Aug 2015 8:02:19 -0700 Subject: [Histonet] Harford online HT program Message-ID: <20150828110219.ATAAM.62559.imail@fed1rmwml214> Hi Netters! Happy Friday! My assistant is starting the Harford HT program online and we are a Dermatology lab, program requires a few different normal tissues like lumen, Uterus, Gallbladder, etc.. was wondering if anyone out there has taken same or similar course and how did you acquire your tissue if in a GI or Derm lab? All we have is Derm tissue, is there a tissue bank out there somewhere? I thought I heard mention in the past. Or where can you buy tissue? Any information is greatly appreciated!! Thank you in advance!! From elaineahoffman55 at yahoo.com Fri Aug 28 10:12:11 2015 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Fri, 28 Aug 2015 15:12:11 +0000 (UTC) Subject: [Histonet] CLIA regulations in Histology Message-ID: <760454168.2225106.1440774731450.JavaMail.yahoo@mail.yahoo.com> Under CLIA regulations, all six elements of competency assessment must be completed for every test a person performs, not just testing in general.Also while CLIA does not specifically require assessment of the competency of personnel performing only pre-analytic and post-analytic activities, it is good practice to do so. I have several questions in regards to these statements:? What is considered "testing" each person performs specifically in the histology lab?? What examples of pre-analytic and post-analytic activities are performed in the histology lab?? There are so many examples given for the clinical side of the lab but virtually none given for histology.? Yet we are expected to carry out this requirement.? I got several citations in this area during my last CLIA inspection.? So now I'm required to re-write my Competency Assessment Policy, which I don't really know how to do.? If you ask the CLIA inspectors, they just tell you to go on-line and check out the guidelines which are so vague and confusing and don't give you any information on histology.? I need specific histology examples of measurable/observable criteria that could be acceptable for each of the six assessment methods.? We are just a tiny GI histology lab doing biopsies from a private physicians' GI scoping facility.? I was told that H&E staining was not considered "testing", neither is embedding, microtomy, coverslipping, etc.? I don't know what is considered pre-analytic or post-analytic activities in histology either.? The guidelines given are so general and wide-ranging.? I need specifics that pertain to histology. Another citation was also in the area where the pathologists "read" the slides.? I'm required to re-write the competency assessment procedure on our pathologists since they are do provider performed microscopy (PPM) testing.? I need specific examples to do competency assessments on them as well.? I know their "testing" is reading slides under the microscope but I need to observe all 6 assessment methods.? Proficiency testing on pathologists??? I really need to take a course or class on competency assessments, proficiency testing, pre-analytic and post-analytic activities.? The guideline language is too confusing.? Any suggestions or information would be greatly appreciated.? Thanks.... Elaine Hoffman, HT(ASCP) The Gastroenterology Clinic & Endoscopy Center, Inc.Warren, Ohio 44483 From kdonadio at ymail.com Fri Aug 28 13:02:37 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Fri, 28 Aug 2015 18:02:37 +0000 (UTC) Subject: [Histonet] CLIA regulations in Histology In-Reply-To: <760454168.2225106.1440774731450.JavaMail.yahoo@mail.yahoo.com> References: <760454168.2225106.1440774731450.JavaMail.yahoo@mail.yahoo.com> Message-ID: <365460905.2349908.1440784957796.JavaMail.yahoo@mail.yahoo.com> I always added competency for my staff in their annual evaluations. It did include:Accessions specimens accurately ( pre-analytical)-ie: specimen handlingKnows how to start and maintain tissue processor with competency ( pre-analytical)Grosses specimens accurately ( analytical) and high complexity?Embeds tissue samples with accuracy ( analytical)Performs microtomy with few errors and acceptable quality ( analytical)Maintains H&E stain line with competency ( analytical)- also should have PT 2 times a year for H&E quality. purchasePerforms?special stains with quality results and few errors ( analytical)Performs?IHC with quality results and few errors ( analytical and high complexity)- also need PT 2 times a year. purchaseCoverslips and labels slides with few errors ( post analytical) -case assemblyPerforms transcription with few errors ( post-analytical) Performs frozen sections with high degree of quality ( analytical)Performs frozen sections 90% of time under 20 mins ( analytical)?Above is many, not all that I've always included with my annual evaluation. In which you (the supervisor) and the Medical director sign and verify that you have observed your staff and monitored them during these procedures. This was part of?the Quality assurance program and monitored daily under the Quality Management program. ie; keeping track of any errors, repeats, problems?and computing that data into measurable stats. < Reviewing data. ?Problem solving skills: Data for this can be extracted from your QM logs at "How was the error fixed?", which should be recorded in your QM data. ?So while CLIA may not regulate( as far as?extra educational requirements)?microtomy, embedding, routine staining are certainly things you need to show competency on for your staff. ?As far as PPM's go,the providers had a test for competency that they had to have another provider check off on and sign that they observed the other provider performing the test and?were competent in that test. They also have to have for each PPM type test they did 2 PT ( proficiency test) a year, 6 months apart. Either by second reviews, purchase from outside source or in house quiz. ?For pathologist reading slides, have another qualified pathologist do second reviews for?so many cases a year. 2-3% usually was the amount?they chose.??ie: < Blind reviews. ?Sorry this is so long. It should be longer but that's a bunch your asking and I tried to summarize best I could. I'm sure many others can add to this as its a big subject and honestly requires a lot. ?I recommend purchasing a CLIA manual. And I'm not sure but Anderson used to offer a 24 CE course on CLIA. I took it but that was back when rocks was money. :) ?Hope this helps. ?Kim D ??? From: Elaine allison Hoffman via Histonet To: Histonet List Sent: Friday, August 28, 2015 10:12 AM Subject: [Histonet] CLIA regulations in Histology Under CLIA regulations, all six elements of competency assessment must be completed for every test a person performs, not just testing in general.Also while CLIA does not specifically require assessment of the competency of personnel performing only pre-analytic and post-analytic activities, it is good practice to do so. I have several questions in regards to these statements:? What is considered "testing" each person performs specifically in the histology lab?? What examples of pre-analytic and post-analytic activities are performed in the histology lab?? There are so many examples given for the clinical side of the lab but virtually none given for histology.? Yet we are expected to carry out this requirement.? I got several citations in this area during my last CLIA inspection.? So now I'm required to re-write my Competency Assessment Policy, which I don't really know how to do.? If you ask the CLIA inspectors, they just tell you to go on-line and check out the guidelines which are so vague and confusing and don't give you any information on histology.? I need specific histology examples of measurable/observable criteria that could be acceptable for each of the six assessment methods.? We are just a tiny GI histology lab doing biopsies from a private physicians' GI scoping facility.? I was told that H&E staining was not considered "testing", neither is embedding, microtomy, coverslipping, etc.? I don't know what is considered pre-analytic or post-analytic activities in histology either.? The guidelines given are so general and wide-ranging.? I need specifics that pertain to histology. Another citation was also in the area where the pathologists "read" the slides.? I'm required to re-write the competency assessment procedure on our pathologists since they are do provider performed microscopy (PPM) testing.? I need specific examples to do competency assessments on them as well.? I know their "testing" is reading slides under the microscope but I need to observe all 6 assessment methods.? Proficiency testing on pathologists??? I really need to take a course or class on competency assessments, proficiency testing, pre-analytic and post-analytic activities.? The guideline language is too confusing.? Any suggestions or information would be greatly appreciated.? Thanks.... Elaine Hoffman, HT(ASCP) The Gastroenterology Clinic & Endoscopy Center, Inc.Warren, Ohio 44483 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Fri Aug 28 13:05:17 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 28 Aug 2015 18:05:17 +0000 Subject: [Histonet] Frozen Section WL and Billing In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F802BA6C@HRHEX02-HOS.holyredeemer.local> We set up our system this way - Example: A specimen for frozen is sent. You cut 2 blocks of frozen tissue, 2 levels ea. The PA/Pathologist submits 3 additional blocks for a total of 5 blocks on the specimen (1-2 are the previously frozen, 3-5 additional tissue) to be cut at one level each. 1. At accession, the technical bill for the Lev 4 gross and micro (88305) drop based on the specimen type (skin biopsy) 2. The tech enters a protocol for 5 blocks, 1 H&E stain each block. 3. The tech modifies the stains, and changes the H&E on the first block to a stain called FS1 (Frozen Section, first block) The charge for the first frozen block, 88341, drops in when this stain is ordered 4. The tech modifies the stains, and changes the H&E on the second block to a stain called FSA (Frozen Section, Additional Block) The charge for the additional frozen block, 88342, drops in when this stain is ordered. 5. The tech then enters stains that are set up in the stain dictionary as a "Label Only". There is no charge associated with these stains. Stain FL1, Block 1, Stain FL2, Block 1, Stain FL1, Block 2, Stain FL2, Block 2, 6. FL1 = Frozen Label, 1st level FL2 = Frozen Label, 2nd level 7. You can define as many of these Frozen Label levels as you will ever need. 8. Print the labels for the case (some systems will allow you to set up a print job for by Label Type, and you can select "Labels Only") It sounds a bit complicated to set up, but the steps are simple, and once it has been set up to use this way, it is quick, easy and accurate to use. I don't know what LIS system you are using, but this is easily adaptable for almost any system We also set up the system to include the "Label Only" slides to be included in the total slide count, so you get work credit there. I hope this helps. Please feel free to call or contact for any questions. Also, I'm not that far from you, if you need a little help. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. frozen section- workload and billing (Davis, Cassie) From: "Davis, Cassie" To: "histonet at lists.utsouthwestern.edu" Hi Histofolks, I need to pick yours brains...we are in the middle of building a workable computer system for our lab we have run into a hiccup when it come to frozen sections. As a tech I know there is actual hands on, stop what you are doing, do this now work involved. My understanding from a billing perspective it is not "billable workload" but an "interdepartmental consultation between surgery and pathology". The problem is how to build the system so we get labels for our frozen section slides that does not interfere with the "billable workload" that is. I was thinking maybe it should be built in the system the same way a control slide is, does anybody have any suggestions? Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org From adhall at ael.com Fri Aug 28 13:15:56 2015 From: adhall at ael.com (Angela Hall) Date: Fri, 28 Aug 2015 13:15:56 -0500 Subject: [Histonet] Harford online HT program In-Reply-To: References: Message-ID: Dear future Hartford HT online student, This is a great course. Between the course work that they sent, Freida Carson's text, workbook, and flip cards, I was able to pass the ASCP test on my first attempt!!!! My online mentor and my in-house lab mentor were the also great with both encouragement and advice. But you will have to keep an on time because it's easy to get behind when you're not constantly in a class room. I set up reminders on my email to help me keep on track I don't know of any tissue banks but there was very little tissue that we didn't have on hand in our anatomic pathology lab. Also, you don't have to use human tissue for all of it. If you have a medical or vet school in the area, check with them to see if they are willing to work with you on tissue for your courses. You might check with an anatomic lab to see if they might be able to work out an agreement for any tissue that they are discarding into medical waste. Good luck. Angela D. Hall, BA, HT(ASCP)CM -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, August 28, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 141, Issue 26 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From robinsoc at mercyhealth.com Fri Aug 28 14:23:50 2015 From: robinsoc at mercyhealth.com (Cynthia Robinson) Date: Fri, 28 Aug 2015 19:23:50 +0000 Subject: [Histonet] Frozen Section WL and Billing In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F802BA6C@HRHEX02-HOS.holyredeemer.local> References: , <48E053DDF6CE074DB6A7414BA05403F802BA6C@HRHEX02-HOS.holyredeemer.local> Message-ID: <4EE642D353925D4D96CB95E12427DBAE45E52410@NODCMSTMBX06.no.trinity-health.org> Aren't fs CPT codes 88331 and 88332? ________________________________________ From: Terri Braud via Histonet [histonet at lists.utsouthwestern.edu] Sent: Friday, August 28, 2015 1:05 PM To: histonet at lists.utsouthwestern.edu Cc: Cassandra P. Davis Subject: Re: [Histonet] Frozen Section WL and Billing We set up our system this way - Example: A specimen for frozen is sent. You cut 2 blocks of frozen tissue, 2 levels ea. The PA/Pathologist submits 3 additional blocks for a total of 5 blocks on the specimen (1-2 are the previously frozen, 3-5 additional tissue) to be cut at one level each. 1. At accession, the technical bill for the Lev 4 gross and micro (88305) drop based on the specimen type (skin biopsy) 2. The tech enters a protocol for 5 blocks, 1 H&E stain each block. 3. The tech modifies the stains, and changes the H&E on the first block to a stain called FS1 (Frozen Section, first block) The charge for the first frozen block, 88341, drops in when this stain is ordered 4. The tech modifies the stains, and changes the H&E on the second block to a stain called FSA (Frozen Section, Additional Block) The charge for the additional frozen block, 88342, drops in when this stain is ordered. 5. The tech then enters stains that are set up in the stain dictionary as a "Label Only". There is no charge associated with these stains. Stain FL1, Block 1, Stain FL2, Block 1, Stain FL1, Block 2, Stain FL2, Block 2, 6. FL1 = Frozen Label, 1st level FL2 = Frozen Label, 2nd level 7. You can define as many of these Frozen Label levels as you will ever need. 8. Print the labels for the case (some systems will allow you to set up a print job for by Label Type, and you can select "Labels Only") It sounds a bit complicated to set up, but the steps are simple, and once it has been set up to use this way, it is quick, easy and accurate to use. I don't know what LIS system you are using, but this is easily adaptable for almost any system We also set up the system to include the "Label Only" slides to be included in the total slide count, so you get work credit there. I hope this helps. Please feel free to call or contact for any questions. Also, I'm not that far from you, if you need a little help. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. frozen section- workload and billing (Davis, Cassie) From: "Davis, Cassie" To: "histonet at lists.utsouthwestern.edu" Hi Histofolks, I need to pick yours brains...we are in the middle of building a workable computer system for our lab we have run into a hiccup when it come to frozen sections. As a tech I know there is actual hands on, stop what you are doing, do this now work involved. My understanding from a billing perspective it is not "billable workload" but an "interdepartmental consultation between surgery and pathology". The problem is how to build the system so we get labels for our frozen section slides that does not interfere with the "billable workload" that is. I was thinking maybe it should be built in the system the same way a control slide is, does anybody have any suggestions? Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tbraud at holyredeemer.com Fri Aug 28 14:24:41 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 28 Aug 2015 19:24:41 +0000 Subject: [Histonet] Frozen Section WL and Billing In-Reply-To: <4EE642D353925D4D96CB95E12427DBAE45E52410@NODCMSTMBX06.no.trinity-health.org> References: , <48E053DDF6CE074DB6A7414BA05403F802BA6C@HRHEX02-HOS.holyredeemer.local> <4EE642D353925D4D96CB95E12427DBAE45E52410@NODCMSTMBX06.no.trinity-health.org> Message-ID: <48E053DDF6CE074DB6A7414BA05403F802BAAB@HRHEX02-HOS.holyredeemer.local> ACK! Yep, my bad Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: Cynthia Robinson [mailto:robinsoc at mercyhealth.com] Sent: Friday, August 28, 2015 3:24 PM To: Terri Braud; histonet at lists.utsouthwestern.edu Cc: Cassandra P. Davis Subject: RE: Frozen Section WL and Billing Aren't fs CPT codes 88331 and 88332? ________________________________________ From: Terri Braud via Histonet [histonet at lists.utsouthwestern.edu] Sent: Friday, August 28, 2015 1:05 PM To: histonet at lists.utsouthwestern.edu Cc: Cassandra P. Davis Subject: Re: [Histonet] Frozen Section WL and Billing We set up our system this way - Example: A specimen for frozen is sent. You cut 2 blocks of frozen tissue, 2 levels ea. The PA/Pathologist submits 3 additional blocks for a total of 5 blocks on the specimen (1-2 are the previously frozen, 3-5 additional tissue) to be cut at one level each. 1. At accession, the technical bill for the Lev 4 gross and micro (88305) drop based on the specimen type (skin biopsy) 2. The tech enters a protocol for 5 blocks, 1 H&E stain each block. 3. The tech modifies the stains, and changes the H&E on the first block to a stain called FS1 (Frozen Section, first block) The charge for the first frozen block, 88341, drops in when this stain is ordered 4. The tech modifies the stains, and changes the H&E on the second block to a stain called FSA (Frozen Section, Additional Block) The charge for the additional frozen block, 88342, drops in when this stain is ordered. 5. The tech then enters stains that are set up in the stain dictionary as a "Label Only". There is no charge associated with these stains. Stain FL1, Block 1, Stain FL2, Block 1, Stain FL1, Block 2, Stain FL2, Block 2, 6. FL1 = Frozen Label, 1st level FL2 = Frozen Label, 2nd level 7. You can define as many of these Frozen Label levels as you will ever need. 8. Print the labels for the case (some systems will allow you to set up a print job for by Label Type, and you can select "Labels Only") It sounds a bit complicated to set up, but the steps are simple, and once it has been set up to use this way, it is quick, easy and accurate to use. I don't know what LIS system you are using, but this is easily adaptable for almost any system We also set up the system to include the "Label Only" slides to be included in the total slide count, so you get work credit there. I hope this helps. Please feel free to call or contact for any questions. Also, I'm not that far from you, if you need a little help. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. frozen section- workload and billing (Davis, Cassie) From: "Davis, Cassie" To: "histonet at lists.utsouthwestern.edu" Hi Histofolks, I need to pick yours brains...we are in the middle of building a workable computer system for our lab we have run into a hiccup when it come to frozen sections. As a tech I know there is actual hands on, stop what you are doing, do this now work involved. My understanding from a billing perspective it is not "billable workload" but an "interdepartmental consultation between surgery and pathology". The problem is how to build the system so we get labels for our frozen section slides that does not interfere with the "billable workload" that is. I was thinking maybe it should be built in the system the same way a control slide is, does anybody have any suggestions? Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From KSimeone at leavittmgt.com Mon Aug 31 08:49:36 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 31 Aug 2015 13:49:36 +0000 Subject: [Histonet] FULLTIME DAY SHIFT (first shift) POSITION DELRAY BCH FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C4D2DCE8A@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C4D2DCE8A@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DD06E@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Beach, Florida dermatology laboratory. This is a permanent full time, DAYTIME SHIFT (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***WE DO NOT HIRE TEMPS!!*** PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html *full time position Mon-Fri 8a-5:30p (start time may vary) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From jerrysedgewick at gmail.com Mon Aug 31 11:19:54 2015 From: jerrysedgewick at gmail.com (J. Sedgewick) Date: Mon, 31 Aug 2015 11:19:54 -0500 Subject: [Histonet] =?utf-8?b?TmV4dCB3ZWVrIOKAkyBmcmVlIHdlYmluYXI6IOKAnDUg?= =?utf-8?q?Common_Mistakes_when_using_Photoshop_for_Science?= =?utf-8?b?4oCd?= Message-ID: 5 Common Mistakes when using Photoshop for Science: Tips, Tricks and a Case Study Sep 9, 2015 1:00 PM EDT at: https://attendee.gotowebinar.com/register/2382965322253067778 Images are a vital component of scientific research for evaluation, data analysis, visualizing the impact of experimental parameters, and in medicine for assessing disease conditions. Regardless of the equipment price tag or level of automation, microscope systems generally fall short in providing images with consistent quality optimized for color, contrast and brightness. Adobe? Photoshop? or other image editing software is commonly used to correct for image insufficiencies, but consequences exist. Join our speakers as they review the common mistakes encountered when using Photoshop for science, offer tips to achieve consistency and high quality in your imaging process, and share their personal experience on how to avoid these common mistakes altogether. This webinar is co-sponsored by Imaging and Analysis, LLC and Datacolor Inc. About the speakers: Jerry Sedgewick (Imaging and Analysis, LLC), is an imaging consultant, an image forensic specialist, and a former core microscopy facility manager. As a recognized authority in applying Photoshop to edit scientific images, and through his books, articles and workshops, Mr. Sedgewick has helped hundreds of researchers use Photoshop to achieve their desired results. Dr. Dawn M. Dawson received her M.D. from Eastern Virginia Medical School in 1989, followed by a residency and fellowship in Anatomic and Clinical Pathology at the Cleveland Clinic Foundation, receiving Board Certification in 1996. From 1998 until 2000, Dr. Dawson was a fellow in the Translational Research in Oncology Training Program. Currently, she is an Assistant Professor at Case Western Reserve University's Institute of Pathology and her responsibilities include immunohistochemistry activities. Her current research focuses on growth factor/receptors and cell signaling in carcinogenesis models. From k84as at yahoo.com Mon Aug 31 14:44:33 2015 From: k84as at yahoo.com (mohamed abd el razik) Date: Mon, 31 Aug 2015 19:44:33 +0000 (UTC) Subject: [Histonet] paraffin dispenser help! Message-ID: <1274861741.2382003.1441050273807.JavaMail.yahoo@mail.yahoo.com> Hi all i'm in urgent need for operating SHANDON LIPSHAW PARAFFIN DISPENSER MODEL 224 how to start using it please in simple stepsthanks in advance mohamed From ASelf at tidelandshealth.org Mon Aug 31 15:41:10 2015 From: ASelf at tidelandshealth.org (Amy Self) Date: Mon, 31 Aug 2015 16:41:10 -0400 Subject: [Histonet] Histology Workload Message-ID: Happy Monday to Everyone, I have been trying[ to figure out how to justify additional help in histology - and it's been hard. My facility staffs according to billables. This by no means feels fair to anyone in this department. You could have a specimen that will provide one billable CPT code but can produce as many as 20 paraffin blocks. And now it seems like we are getting many request for molecular test that require us to mail the patients material out but we have no way of showing that we did this work - we call this free work. We get no credit for time spent preparing and packaging pathology material to send out to reference labs. Am I missing any CPT codes that can be used to show that we in fact did something in addition to routine pathology to this case. We have 1 histotech - 1 histotech/histology supervisor and one histology assistant. Our block load averages from 125 to 170 daily. We also prep non-gyn cytologies. Accession all specimens that come in that lab. All mail-outs The histotech/histology supervisor is responsible for all of the billing as well as keeping up with new and old policies/ requirements for CAP/ auditing billing and the list could go on. We are drowning in our own workload but don't know how to prove that the help is needed. Any help - advice - suggestions anything will be appreciated. How can I prove to upper management that we need more help although the billables/productivity numbers say different? Thanks in advance, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From Richard.Cartun at hhchealth.org Mon Aug 31 16:26:17 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 31 Aug 2015 21:26:17 +0000 Subject: [Histonet] Histology Workload In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B03DECC@HHCEXCHMB03.hhcsystem.org> Dear Amy: Some of the companies doing molecular testing will pay you a fee for "specimen procurement/handling" since they realize the burden that these requests put on us. I suggest calling the company(s) that you deal with and see if you can work something out with them. And, you're absolutely correct; we are being asked to do more work on specimens today, yet reimbursements are going down. Not a good combination. Based on your description of the work that you and your colleagues are doing, you have a good case for additional staffing. It looks like you would benefit from a lab assistant that could help with accessioning, billing, and send-outs. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, August 31, 2015 4:41 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Histology Workload Happy Monday to Everyone, I have been trying[ to figure out how to justify additional help in histology - and it's been hard. My facility staffs according to billables. This by no means feels fair to anyone in this department. You could have a specimen that will provide one billable CPT code but can produce as many as 20 paraffin blocks. And now it seems like we are getting many request for molecular test that require us to mail the patients material out but we have no way of showing that we did this work - we call this free work. We get no credit for time spent preparing and packaging pathology material to send out to reference labs. Am I missing any CPT codes that can be used to show that we in fact did something in addition to routine pathology to this case. We have 1 histotech - 1 histotech/histology supervisor and one histology assistant. Our block load averages from 125 to 170 daily. We also prep non-gyn cytologies. Accession all specimens that come in that lab. All mail-outs The histotech/histology supervisor is responsible for all of the billing as well as keeping up with new and old policies/ requirements for CAP/ auditing billing and the list could go on. We are drowning in our own workload but don't know how to prove that the help is needed. Any help - advice - suggestions anything will be appreciated. How can I prove to upper management that we need more help although the billables/productivity numbers say different? Thanks in advance, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.