[Histonet] AEC visualization enhancement

[平田勝啓(HirataMasahiro)]

Hi,

Can anyone please let me know how to increase AEC sensitivity ?

I am using AEC for multiple IHC, but it is not particularly sensitive.

My recepe is:
1% AEC in DMF 0.5ml
50mM acetate/sodium acetate buffer pH5.5 10ml
30% H2O2 5ul
mix and filter
incubate for 10-15min

I m looking for enhancer (like imidazole for DAB) or more sensitive
protocol for AEC.
Thank you

​Hirata Masahiro
Department of Diagnostic Pathology, Kyoto University Hospital

hiratama <@t> kuhp.kyoto-u.ac.jp