[Histonet] Intestinal bacteria for paraffin sections - morphology?

[Mikael Niku]

Hi!

I'm trying to produce paraffin sections showing intestinal bacteria in 
intestinal tissue sections. We have tried routine PFA fixation and 
paraffin processing, and a Carnoy-based protocol supposedly optimized 
for this purpose. The problem is, the bacteria seem to shrink so much 
that I can't see any nice shapes but only very tiny roughly circular 
objects. What to do?

With best regards,
Mikael Niku, University of Helsinki