[Histonet] RE: ORO tissue falling off

[Grantham, Andrea L - (algranth)]

I used to be the Queen of ORO in my lab at the University of Arizona. I had some of the fattiest livers that could be possible from different research projects. I never had a problem with the livers staying on the slide. I used Epic coated slides or Stat Lab slides and the protocol was from Freida's second edition. The stain was from PolyScientific R&D.
I cut the sections at 5 microns, let them sit in the cryostat for about 30 minutes and then fixed in 37% Formaldehyde for about 10-30 minutes - never really timed it. All steps of staining were done gently, one slide at a time, no matter how many slides I had to stain.
Sections were beautiful - wish I could post a picture here.
Retired now - sometimes I miss doing things like this.

Andi Grantham
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Linda Prasad (SCHN) [linda.prasad <@t> health.nsw.gov.au]
Sent: Wednesday, April 15, 2015 5:07 PM
To: 'Jo-Ann Bader, Ms.'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: ORO tissue falling off

Usually with the fatty tissues, I pick them up on superfrost slides and let it air dry  for 2-3 days at room temperature and then perform the ORO stains. So far they seem to stay on.

Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad <@t> health.nsw.gov.au | w: www.schn.health.nsw.gov.au

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms.
Sent: Thursday, 16 April 2015 1:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ORO tissue falling off

We are having difficulty with a particulate set of very, very fatty mouse livers.  The normal livers from this set stay on the slides the fatty livers fall off.  We have used different types of charged slides and we have even tried to drench the charged slides in Stay-On, dry them and then put the frozen tissues on (despirate times call for despirate measures).  No luck  Does anyone have any other ideas.  Help Help

Jo-Ann  Bader
Histology Coordinator
Goodman Cancer Research Center
1600 Pine Ave. W,
Room 312
Montreal Quebec, H3A 1A3
Email: jo-ann.bader <@t> mcgill.ca<mailto:jo-ann.bader <@t> mcgill.ca>
Office Tel:  514-398-5647
Lab:  Tel:  514-398-8270

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