[Histonet] Paraffin embedding of whole mouse brain

[koellingr <@t> comcast.net]

Hi, could you cut the brain in half (in coronal cut orientation).  Using the great mouse brain molds available you could cut "in half" the brain at exactly the spot you wanted.  So have two 6-7 mm blocks both in coronal sectioning orientation and you can cut as you wish from the two blocks.  Alternatively, on the Allen Brain Atlas page they have coronal sections of mouse brain (132 of them cut at 100um intervals so I guess that is your 132x100=13,200 um- or 13 mm).  That wouldn't be a trade secret if you asked them. 
  
Ray, 
Lake Forest Park, WA 

----- Original Message -----

From: "Ramakrishna Samala" <ramakrishna.samala <@t> ttuhsc.edu> 
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Friday, April 10, 2015 7:00:27 AM 
Subject: [Histonet] Paraffin embedding of whole mouse brain 

Hello Histonet! 

I would like to paraffin embed whole mouse brain to obtain coronal sections. I spoke with customer support teams of several companies, I could able to get 10 mm deep molds,  but the mouse brain is about 13 mm height. So, any help in this regard is much appreciated. 

Sincerely 

Ramakrishna Samala, Ph.D. 
Senior Research Associate 
Amarillo Research Building, School of Pharmacy, Texas Tech University Health Sciences Center 
1406 S. Coulter 
Amarillo, TX 79106 

The outcome of any serious research can only be to make two questions grow where only one grew before –Thorstein Veblen 


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