From thigginsht <@t> msn.com Wed Apr 1 09:51:39 2015 From: thigginsht <@t> msn.com (Tim H) Date: Wed Apr 1 09:51:43 2015 Subject: [Histonet] Allowable temperature range Message-ID: What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage? Any ideas? Tim From Nancy_Schmitt <@t> pa-ucl.com Wed Apr 1 10:26:38 2015 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Apr 1 10:26:45 2015 Subject: [Histonet] waterbath/faucet disinfection Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3601159C57ED@PEITHA.wad.pa-ucl.com> I am curious to know what others are doing (if anything) to disinfect their faucets/waterbaths on a daily basis. Our procedure uses bleach on both daily - that will now change as the bleach will ruin the finish on our newer waterbaths. Thanks for your input- Nancy Schmitt MLT, HT(ASCP) Histology Coordinator NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From juc2023 <@t> med.cornell.edu Wed Apr 1 11:06:03 2015 From: juc2023 <@t> med.cornell.edu (Julie Cohen) Date: Wed Apr 1 11:07:21 2015 Subject: [Histonet] inconsistent H&E staining Message-ID: Hi, I made slides of paraffin-embedded mouse small intestine (Swiss rolls), and stained them with Hematoxylin and Eosin. Parts of the tissue on the same slide are stained dark with good structure. Other areas look washed out with poor structure. We realize that some of this could be caused by the orientation/structures captured, but similar tissue type looks paler as well. Has anyone had a similar experience, and could suggest an explanation for me to give to our client? At first we thought it might be due to poor fixation, since the centers of tightly-wound rolls were affected, but we also observed this in the outer parts of loosely wound rolls. I soak the blocks before sectioning; could non-uniform swelling result in variations of the section thickness? (These are 7 microns thick.) Apologies if this information is available somewhere else; I tried unsuccessfully searching the archives. Thank you, Julie Cohen Research Lab Tech EM Core Facility Weill Cornell Medical College 1300 York Avenue, Room A-105 New York, NY 10021 lab: 212-746-6146 email: juc2023@med.cornell.edu From mcauliff <@t> rwjms.rutgers.edu Wed Apr 1 11:27:50 2015 From: mcauliff <@t> rwjms.rutgers.edu (Geoff) Date: Wed Apr 1 11:28:04 2015 Subject: [Histonet] inconsistent H&E staining In-Reply-To: References: Message-ID: <551C1C86.2050304@umdnj.edu> Failure to completely remove paraffin is often a cause of uneven staining. Change all of the xylenes and add another xylene. Geoff On 4/1/2015 12:06 PM, Julie Cohen wrote: > Hi, > > I made slides of paraffin-embedded mouse small intestine (Swiss rolls), and stained them with Hematoxylin and Eosin. Parts of the tissue on the same slide are stained dark with good structure. Other areas look washed out with poor structure. We realize that some of this could be caused by the orientation/structures captured, but similar tissue type looks paler as well. > > Has anyone had a similar experience, and could suggest an explanation for me to give to our client? At first we thought it might be due to poor fixation, since the centers of tightly-wound rolls were affected, but we also observed this in the outer parts of loosely wound rolls. I soak the blocks before sectioning; could non-uniform swelling result in variations of the section thickness? (These are 7 microns thick.) > > Apologies if this information is available somewhere else; I tried unsuccessfully searching the archives. > > Thank you, > > Julie Cohen > > Research Lab Tech > EM Core Facility > Weill Cornell Medical College > 1300 York Avenue, Room A-105 > New York, NY 10021 > lab: 212-746-6146 > email: juc2023@med.cornell.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff@rwjms.rutgers.edu ********************************************** From kkienitz <@t> orclinic.com Wed Apr 1 11:29:20 2015 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Wed Apr 1 11:32:16 2015 Subject: [Histonet] RE: inconsistent H&E staining In-Reply-To: References: Message-ID: <41400FFE517878449D89114DD25260901960F22E97@tocmail1.tocad.orclinic.com> Hi Julie, Try increasing your deparaffinization times, sometimes there is just not enough time in xylene so subsequent staining can be very inconsistent like described. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julie Cohen [juc2023@med.cornell.edu] Sent: Wednesday, April 01, 2015 9:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] inconsistent H&E staining Hi, I made slides of paraffin-embedded mouse small intestine (Swiss rolls), and stained them with Hematoxylin and Eosin. Parts of the tissue on the same slide are stained dark with good structure. Other areas look washed out with poor structure. We realize that some of this could be caused by the orientation/structures captured, but similar tissue type looks paler as well. Has anyone had a similar experience, and could suggest an explanation for me to give to our client? At first we thought it might be due to poor fixation, since the centers of tightly-wound rolls were affected, but we also observed this in the outer parts of loosely wound rolls. I soak the blocks before sectioning; could non-uniform swelling result in variations of the section thickness? (These are 7 microns thick.) Apologies if this information is available somewhere else; I tried unsuccessfully searching the archives. Thank you, Julie Cohen Research Lab Tech EM Core Facility Weill Cornell Medical College 1300 York Avenue, Room A-105 New York, NY 10021 lab: 212-746-6146 email: juc2023@med.cornell.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tejohnson <@t> genoptix.com Wed Apr 1 12:12:05 2015 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Wed Apr 1 12:12:16 2015 Subject: [Histonet] Re: inconsistent H&E staining Message-ID: <6f3c415fc180434180917efe9240f3fc@PHUSCB-SP37MB03.genoptix.org> Julie, Are the inconsistencies consistent among different sections? In other words, with additional sections, are you seeing the paler areas in the same places or different ones? If you section it today, are the pale areas in the same place as when you sectioned it previously? If it is the same area across multiple sections it is likely your tissues and not inadequate deparaffinization. Drying artifact is often seen in epithelial tissue structures and can happen when the tissue is out of fixative or have been placed in alcohol (which dehydrates much more quickly) and then made into a swiss roll. It can be a huge problem with GI biopsies if they are placed on dry gauze or paper towel prior to being placed in fixative at the time of biopsy. In my experience, there is not much you can do to fix it. Just be aware of keeping your tissues wet when creating the next swiss roll sample. Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From julie.hinsinger <@t> umontreal.ca Wed Apr 1 12:19:19 2015 From: julie.hinsinger <@t> umontreal.ca (Hinsinger Julie) Date: Wed Apr 1 12:20:18 2015 Subject: [Histonet] DAKO Omnis vs Ventana Ultra Message-ID: <4E1A2BAF76DB1E4CB35AA132AE0492D2201CEDB7@asphodel-cour.sim.umontreal.ca> Hi, Does anyone know about some lab using DAKO Omnis for research ? If some of you tried the Omnis and the Ultra and would like to share about their own experience, it would be highly appreciated. LCS vs dynamic gap staining ? Software usage ? Costs ? Service ? Reagents and kits ? Maintenance, decontamination ? Best regards, Julie Hinsinger Histology Facility Institute for Research in Immunology and Cancer (IRIC) _Universit? de Montr?al 2950, chemin Polytechnique Pavillon Marcelle Coutu - Local 3440 H3T 1J4, Montr?al, QC (514) 343-6111 #0503 julie.hinsinger@umontreal.ca From Maxim_71 <@t> mail.ru Wed Apr 1 12:22:58 2015 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Wed Apr 1 12:23:17 2015 Subject: [Histonet] inconsistent H&E staining Message-ID: <1045908867.20150401202258@mail.ru> Julie, Please, check the temperature around stainer or staining bath. Dapaffinization will be poor or incompletely (as in youre case) in the cold (under 18oC) room. -- Sincerely, Maxim Peshkov, Russia, Taganrog. You wrote: Hi, I made slides of paraffin-embedded mouse small intestine (Swiss rolls), and stained them with Hematoxylin and Eosin. Parts of the tissue on the same slide are stained dark with good structure. Other areas look washed out with poor structure. We realize that some of this could be caused by the orientation/structures captured, but similar tissue type looks paler as well. Has anyone had a similar experience, and could suggest an explanation for me to give to our client? At first we thought it might be due to poor fixation, since the centers of tightly-wound rolls were affected, but we also observed this in the outer parts of loosely wound rolls. I soak the blocks before sectioning; could non-uniform swelling result in variations of the section thickness? (These are 7 microns thick.) Apologies if this information is available somewhere else; I tried unsuccessfully searching the archives. Thank you, Julie Cohen Research Lab Tech EM Core Facility Weill Cornell Medical College 1300 York Avenue, Room A-105 New York, NY 10021 lab: 212-746-6146 email: juc2023@med.cornell.edu -- ? ?????????, Maxim mailto:Maxim_71@mail.ru From sforeman <@t> labpath.com Wed Apr 1 12:36:49 2015 From: sforeman <@t> labpath.com (Susan Foreman) Date: Wed Apr 1 12:41:33 2015 Subject: [Histonet] DAKO Omnis vs Ventana Ultra In-Reply-To: <4E1A2BAF76DB1E4CB35AA132AE0492D2201CEDB7@asphodel-cour.sim.umontreal.ca> References: <4E1A2BAF76DB1E4CB35AA132AE0492D2201CEDB7@asphodel-cour.sim.umontreal.ca> Message-ID: <001801d06ca2$6f3943b0$4dabcb10$@labpath.com> I'm also interested in following this thread. Thank you for any info -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hinsinger Julie Sent: Wednesday, April 01, 2015 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAKO Omnis vs Ventana Ultra Hi, Does anyone know about some lab using DAKO Omnis for research ? If some of you tried the Omnis and the Ultra and would like to share about their own experience, it would be highly appreciated. LCS vs dynamic gap staining ? Software usage ? Costs ? Service ? Reagents and kits ? Maintenance, decontamination ? Best regards, Julie Hinsinger Histology Facility Institute for Research in Immunology and Cancer (IRIC) _Universit? de Montr?al 2950, chemin Polytechnique Pavillon Marcelle Coutu - Local 3440 H3T 1J4, Montr?al, QC (514) 343-6111 #0503 julie.hinsinger@umontreal.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tina.vanmeter <@t> gmail.com Wed Apr 1 13:30:28 2015 From: tina.vanmeter <@t> gmail.com (Tina Van Meter) Date: Wed Apr 1 13:30:33 2015 Subject: [Histonet] DAKO Omnis vs Ventana Ultra In-Reply-To: <4E1A2BAF76DB1E4CB35AA132AE0492D2201CEDB7@asphodel-cour.sim.umontreal.ca> References: <4E1A2BAF76DB1E4CB35AA132AE0492D2201CEDB7@asphodel-cour.sim.umontreal.ca> Message-ID: Hi Julie, I would go with the most flexible "open" system that is capable of using a wide range of protocols. (ex. antibodies from different vendors, varied section thickness, fixed frozen sections, etc.) Kind regards, Tina Van Meter ? On Wed, Apr 1, 2015 at 1:19 PM, Hinsinger Julie < julie.hinsinger@umontreal.ca> wrote: > Hi, > > Does anyone know about some lab using DAKO Omnis for research ? > If some of you tried the Omnis and the Ultra and would like to share about > their own experience, it would be highly appreciated. > LCS vs dynamic gap staining ? Software usage ? Costs ? Service ? Reagents > and kits ? Maintenance, decontamination ? > > Best regards, > > Julie Hinsinger > Histology Facility > Institute for Research in Immunology and Cancer (IRIC) _Universit? de > Montr?al > 2950, chemin Polytechnique > Pavillon Marcelle Coutu - Local 3440 > H3T 1J4, Montr?al, QC > > (514) 343-6111 #0503 > julie.hinsinger@umontreal.ca > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mills <@t> 3scan.com Wed Apr 1 13:37:04 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Wed Apr 1 13:37:28 2015 Subject: [Histonet] Animal reference material In-Reply-To: References: <1427734201161.3625@animalreferencepathology.com> <1EB8F245A303564EADF12AC7022FA74DD76D521F@novus-ex01> Message-ID: Totally agree with Andi, Staining is not much different, but processing times are much shorter and very varied depending on size and type of tissues. Embryo's being the shortest, and brain (typically) being the longest. The animal processing protocol from NSH was very very useful to me: it is on this page: http://www.nsh.org/content/textbooks-and-manuals NSH VIR Manual By the NSH VIR Committee This manual, developed by the NSH VIR Committee, provides self-help guidelines for animal tissue processing. The manual is a collection of processing protocols and comments about animal tissue processing that have been used in veterinary diagnostic, research, toxicology and marine biology. This book is available through the NSH Marketplace . A discount is available to NSH members. For cutting the tissues can be a bit dry, especially mouse liver, you just need to soak it a bit more than you would human tissue, good luck! yours, Caroline On Tue, Mar 31, 2015 at 10:03 AM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > After working for over twenty years on human tissues in clinical labs I > went to work in a research core lab. I was the only histotech so I had to > learn about animal, insect and plant tissue plus some things like cell > pellets and films. I thank my connections from networking thru NSH and > state societies who always came through with the perfect advice so don't > NOT use this resource. > I found that there wasn't much difference with routine special stains like > trichrome, PAS, LFB, oil red o, etc. You may be asked to do some that > aren't usually done on human tissue. I did a lot of picrosirius red and a > special elastic stain for lung tissue. I was asked to combine some stains > like H&E-alcian blue. It really depends on what the people do who are > bringing you the tissue. Since my lab served researchers I could expect > almost anything! Freida Carson's book is a good book to have on hand. I > liked the second edition most. If you can get your hands on an old AFIP > manual and a Preece get them! > You may find differences with IHC. Can't help you there since that was > done in another lab. > Processing is where the differences lie. Make sure you get some good info > on processing other species. Humason is good and don't forget the NSH has > an Animal Processing Manual. I found a lot of protocols online. I had > different schedules for different species and types of tissue - like mouse > embryo or chick embryo, brains, bones, tiny little things like cultured > mouse ovaries and on and on. > Good luck! > > Andi G. > Happily retired but soon to return to Histo (just a little) > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of Wineman, Terra [ > Terra.Wineman@novusint.com] > Sent: Tuesday, March 31, 2015 6:52 AM > To: Patrick Laurie; Kimberly Marshall > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Animal reference material > > Here is a link I found on google. > https://archive.org/details/animaltissuetech00huma > > Terra Wineman, HTL (ASCP)CM > Research Biologist, Physiology > 636-926-7476 phone > terra.wineman@novusint.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie > Sent: Tuesday, March 31, 2015 8:18 AM > To: Kimberly Marshall > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Animal reference material > > The best that I have used in the past was humason's animal tissue > techniques. I think that there are several editions out there, I used a > 1979 edition. After googling it, I see that there may be a full text > version available online too. > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > > On Mon, Mar 30, 2015 at 12:49 PM, Kimberly Marshall < > Kimberly@animalreferencepathology.com> wrote: > > > ?Happy Monday all... > > > > Writing to ask if any of my fellow histo folks can suggest any > > material/books or any information on staining animal tissue. I am > > bringing many special stains into the lab and being still new to > > animal pathology I would like to see if there is any information out > > there so I can educate myself. > > > > Thanks in advcance > > > > Kimberly > > > > > > > > > > Kimberly Marshall H.T.(ASCP) > > > > Histology/Lab Supervisor > > > > Toll Free 1-800-426-2099 > > > > Fax 801-584-5104 > > > > PO Box 17580 > > > > Salt Lake City, Utah 84107 > > > > www.animalreferencepathology.com > om/> > > > > > > > > Advancing the art and science of veterinary medicine > > > > > > > > [cid:image001.jpg@01CF8F87.A0BD4830] > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller Director of Histology 3Scan.com 415 2187297 From tilycyn <@t> auburn.edu Wed Apr 1 13:39:06 2015 From: tilycyn <@t> auburn.edu (Cynthia Hutchinson) Date: Wed Apr 1 13:39:29 2015 Subject: [Histonet] RE: Histonet Digest, Vol 136, Issue 13 In-Reply-To: <201503111657.t2BGvYaG013902@ducserv12.auburn.edu> References: <201503111657.t2BGvYaG013902@ducserv12.auburn.edu> Message-ID: <2872A2FD0D55EF4EA941EF72CC1ED625C5E1EC6F@exmb3> I can have them ready tonight or tomorrow Cynthia Tily Hutchinson Rsch Asst IV Pathobiology 166 Greene Hall Coll of Vet Med Auburn Univ 36849 (334)844 7020 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 11, 2015 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 136, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. anti-BRAF V600E (VE1) Ventana (Susan Foreman) 2. RE: RE: Old slides. (suetp918) 3. PRN (Kristy Castillo) 4. Re: anti-BRAF V600E (VE1) Ventana (Patrick Laurie) 5. Open histology positions: San Francisco (Cheryl) 6. Direct Hire Histotech South of Tucson, AZ-New Grads Welcome to Apply! (Melissa Owens) 7. Job opportunity (Hunter, Theresa) 8. TDP43, PTG poly (victor_tobias@comcast.net) 9. RE: Re: Bird head stored in 70% alcohol and possible decalcification (Rui TAHARA) 10. BIG day today! (David Kemler) 11. PA (Mike) 12. Re: PA (Jennifer MacDonald) 13. Diff Quik troubleshooting (Nancy Schmitt) 14. THICK AND THIN SECTIONS ? (Klaus Dern) 15. RE: Old slides (Mayer,Toysha N) 16. 5-methylcytosine IHC in tissue (Mariela Chertoff) 17. RE: Masson Trichrome stain (Mayer,Toysha N) 18. RE: Old slides (Marcum, Pamela A) 19. RE: Histonet Digest, Vol 136, Issue 12 (Solis, Bryan) ---------------------------------------------------------------------- Message: 1 Date: Tue, 10 Mar 2015 13:15:05 -0400 From: "Susan Foreman" Subject: [Histonet] anti-BRAF V600E (VE1) Ventana To: Message-ID: <00bd01d05b55$c09b85f0$41d291d0$@labpath.com> Content-Type: text/plain; charset="us-ascii" What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring Bioscience or Ventana? What dilution are you using? Are you utilizing amplification? Expensive ------------------------------ Message: 2 Date: Tue, 10 Mar 2015 13:31:58 -0400 From: suetp918 Subject: RE: [Histonet] RE: Old slides. To: "Gowan,Christie C" , 'Bernice Frederick' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=utf-8 So we actually cut the film around the section and mount to another slide and do pretty much what wascabove mentioned placing upside downband placing on paper towel. ??Actually works pretty good. TJUH Sue Paturzo Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: "Gowan,Christie C" Date:03/09/2015 4:01 PM (GMT-05:00) To: 'Bernice Frederick' , histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Old slides. Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 10 Mar 2015 11:11:54 -0700 From: Kristy Castillo Subject: [Histonet] PRN To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetters, I am looking to fill a PRN Histotech position in the Phoenix area. Does anyone know anyone who is retired and looking for PRN Histology work? Please feel free to e-mail me at the following kcastillo@swskin.net Thank you! ________________________________ This transmission may contain confidential information, some or all of which may be protected health information as defined by the federal Health Insurance Portability & Accountability Act (HIPAA) Privacy Rule. This transmission is intended for the exclusive use of the individual or entity to whom it is addressed and may contain information that is proprietary, privileged, confidential and/or exempt from disclosure under applicable law. If you are not the intended recipient (or an employee or agent responsible for delivering this transmission to the intended recipient), you are hereby notified that any disclosure, dissemination, distribution or copying of this information is strictly prohibited and may be subject to legal restriction or sanction. Please notify the sender by telephone to arrange the return or destruction of the information and all copies. ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 14:48:23 -0400 From: Patrick Laurie Subject: Re: [Histonet] anti-BRAF V600E (VE1) Ventana To: Susan Foreman Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Spring Bioscience is part of Ventana, Spring had the original clone, and now so does Ventana. We use the spring bioscience concentrated antibody on the Bond III at 1:100 dilution, using the Bond Hi-pH ER2 retrieval for 20 minutes. I know that Ventana sells the RTU version. Remember that from Spring it is RUO, so it is up to you to do the appropriate LDT. The Ventana antibody is an IVD. It is also a pretty pricey antibody from either vendor. Let me know if you need any further help. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Mar 10, 2015 at 1:15 PM, Susan Foreman wrote: > What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring > Bioscience or Ventana? What dilution are you using? Are you utilizing > amplification? Expensive > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Tue, 10 Mar 2015 12:37:23 -0700 From: Cheryl Subject: [Histonet] Open histology positions: San Francisco To: histonet@lists.utsouthwestern.edu Message-ID: <1426016243.35673.YahooMailBasic@web161204.mail.bf1.yahoo.com> Content-Type: text/plain; charset=us-ascii Happy Histology Day, everyone! I'm looking for techs for my new (to me) lab!! I'm working at CPMC Sutter in San Francisco and we need a couple of permanent hires for our team. First few days it looks like it's gonna be fun - rock'n'roll kinda place where quality and speed have equal bearing. Let me know what you're looking for and let's see if it's a match! Email resumes to me at the email, below. Please feel free to share and THANK YOU! Cheryl Kerry, HT(ASCP) admin@fullstaff.org ------------------------------ Message: 6 Date: Tue, 10 Mar 2015 20:28:30 +0000 From: Melissa Owens Subject: [Histonet] Direct Hire Histotech South of Tucson, AZ-New Grads Welcome to Apply! To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonet, I am in search of a histotech for a permanent position about an hour south of Tucson, AZ. New grads welcome to apply and relocation assistance is offered. Please contact me for more details. Thank you! Melissa Owens Allied Search Partners ------------------------------ Message: 7 Date: Tue, 10 Mar 2015 20:43:38 +0000 From: "Hunter, Theresa" Subject: [Histonet] Job opportunity To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <38012F015BD58743B1EF391E522AD9B2BC025597@EXMBX4.mshmc.local> Content-Type: text/plain; charset="us-ascii" Hello everyone and happy National Histotechnologist Day! We are looking for a Histology Supervisor to lead a great team in Hershey Pa. If you are interested, please follow the link below. Thanks for your time, Theresa Career Website: http://www.pennstatehershey.org/web/humanresources/home/searchjobs The Penn State Milton S. Hershey Medical Center is committed to affirmative action, equal opportunity and the diversity of its workforce. Equal Opportunity Employer - Minorities/Women/Protected Veterans/Disabled. All individuals (including current employees) selected for a position will undergo a background check appropriate for the position's responsibilities Theresa Hunter, MLS(ASCP)cm Interim Assistant Manager AP Cytology/Decedent care/Histology Lead Pathologist Assistant Department of Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center 500 University Drive, Mail Code H160 Hershey, Pa. 17033 Phone (717) 531-0003 Ext. 287181 Fax (717)531-0831 thunter@hmc.psu.edu ------------------------------ Message: 8 Date: Tue, 10 Mar 2015 20:50:27 +0000 (UTC) From: victor_tobias@comcast.net Subject: [Histonet] TDP43, PTG poly To: histonet@lists.utsouthwestern.edu Message-ID: <680449490.1325591.1426020627172.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 We stain TDP43 on both FFPE and frozen sections. The FFPE are done on the Bond, but we can't seem to get as good results with the frozen sections on the Bond. Our neuropathologist still prefers manually stained frozen sections for TDP43 over ones done on the Bond. We would really like to get it working reliably on the Bond. ??Does anyone have a protocol they would be willing to share for frozen sections on the Bond? Victor University of Washington Medical Center ------------------------------ Message: 9 Date: Wed, 11 Mar 2015 09:21:08 +0900 From: Rui TAHARA Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-2022-jp" Thank you for all the helpful suggestions about this topic. My sample has been in the fixative until it would be decalcified. Thank you again, rui > From: ree3@leicester.ac.uk > To: ruio7@hotmail.com > Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification > Date: Tue, 10 Mar 2015 09:49:00 +0000 > > Leave it in formalin for as long as possible..........................good luck > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rui TAHARA > Sent: 04 March 2015 23:39 > To: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification > > Thank you for helpful suggestions. > I have further questions. > Yes, I have a bird head (probably 1 cm X 1 cm ) stored in 70 % ethanol. > But i have a similar size bird head fixed in 3.7% formalin for over night and am actually processing the head to store in 70% ethanol since my lab is just ordering the decalcifying solution. I need to decalcify this sample later. > But i am wondering if it is better to keep the sample in formalin for a week or so till i get the decalcification solution or i should store it in 70 % ethanol and then fix it for a few days again later? > I am afraid that longer fixative time would affect the sample somehow (e.g. the sample become too rigid?) > > Thank you, > > rui > > > From: gayle.callis@bresnan.net > > To: histonet@lists.utsouthwestern.edu > > Date: Wed, 4 Mar 2015 16:25:28 -0700 > > Subject: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification > > > > You wrote: > > > > > > > > I have an adult bird skull that fixed with formalin and then has been > > stored in 70% ethanol. > > > > I have seen the post that the sample stored in 70% ethanol can be > > walking back through to series of ethanol to water and can be > > decalcified if it needs to be. > > > > > > > > I am wondering if anybody has done this and there is any side effects > > from decalcification after going through dehydration and rehydration > > of a sample compared to a general straight forward protocol from > > decalcification to dehydration? > > > > ********************************************************************** > > ****** > > ********************************************************************** > > ****** > > ******************************** > > > > > > > > I have, in the past, when a weekend arrive, I interrupted acid bone > > decalcification by removing it from acid decalcifier, a quick water > > rinse and immersed into 70% alcohol before returning bone to fresh > > acid decalcifier the next working day. The bones always decalcified > > without problems but I am sure the decalcification took longer since > > partially decalcified bone had to rehydrate. I later learned more > > about dipolar (hope I said that correctly) alcohol slowing and/or stopping ionization of calcium > > and ceased using 70% alcohol to interrupt acid decalcification. I now use > > NBF to interrupt decalcification. Interestingly, I learned the alcohol > > technique from the AFIP bone pathology lab. > > > > > > > > Alcohol is put into Perenyi's nitric acid decalcifying solutions to slow > > down or control very rapid nitric acid decalcification. > > > > > > > > You did not say how big the bird skull was? I suggest immersing the skull > > back into NBF to let it totally rehydrate for several days (depending > > on skull size and if the brain is present). I suggest changing NBF if you > > rehydrate longer than a day. You don't need to go back through an alcohol > > gradient since many processing schedules have tissue samples going from NBF > > directly into 70%. If you leave residual alcohol in the bones, the acid > > decalcification could be slower and hopefully not retarded in any way. > > > > > > > > > > It certainly is worth a try. Good luck. > > > > > > > > Gayle M. Callis > > > > HTL/HT/MT(ASCP) > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Tue, 10 Mar 2015 16:39:35 +0000 (UTC) From: David Kemler Subject: [Histonet] BIG day today! To: Histonet Message-ID: <1358663623.2768352.1426005575141.JavaMail.yahoo@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 That's right, it's OUR day! NSH is doing their thing. Link here: http://www.nsh.org/content/histotechnology-professionals-day . Yours,Dave ------------------------------ Message: 11 Date: Tue, 10 Mar 2015 23:47:25 -0400 From: Mike Subject: [Histonet] PA To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Can you be a pathology assistant with on the job training only? Does it matter if you have a bachelors vs a masters degree in a science not related to pathology or a tech license vs no tech license? Thanks Sent from my iPhone ------------------------------ Message: 12 Date: Tue, 10 Mar 2015 21:17:05 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] PA To: Mike Cc: "Histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="UTF-8" To be a PA you would need to have ASCP certification. There is one route to be eligible for certification. To be eligible for this examination category, an applicant must have a minimum of a baccalaureate degree from a regionally accredited college/university, AND successful completion of a NAACLS accredited Pathologists??? Assistant program within the last five years. Most of the NAACLS programs are Masters degrees. From: Mike To: "Histonet@lists.utsouthwestern.edu" Date: 03/10/2015 08:49 PM Subject: [Histonet] PA Sent by: histonet-bounces@lists.utsouthwestern.edu Can you be a pathology assistant with on the job training only? Does it matter if you have a bachelors vs a masters degree in a science not related to pathology or a tech license vs no tech license? Thanks Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 11 Mar 2015 12:49:32 +0000 From: Nancy Schmitt Subject: [Histonet] Diff Quik troubleshooting To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3601159B7F92@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Good Morning- Random FNA case where the slides turn blue as they dry. We have tried taking them back through stains, destaining and restaining, etc. FNA smears are air-dried when we receive them on plain slides, unfixed. Any thoughts appreciated. Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 14 Date: Wed, 11 Mar 2015 08:52:05 -0400 From: Klaus Dern Subject: [Histonet] THICK AND THIN SECTIONS ? To: histonet Message-ID: Content-Type: text/plain; charset=UTF-8 If you are using one of the following microtomes and are being told the advance mechanism is worn out. ( too much play between spindle and spindle nut ) You could be faced with purchasing a new Microtome. ( No parts availability ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. For Information, contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com ------------------------------ Message: 15 Date: Wed, 11 Mar 2015 15:42:45 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE: Old slides To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D5082D7@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Bernice, Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu ------------------------------ Message: 16 Date: Wed, 11 Mar 2015 12:53:13 -0300 From: Mariela Chertoff Subject: [Histonet] 5-methylcytosine IHC in tissue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi Has anybody experience with 5-metcyt staining in brain tissue? some advice about the HCl and Boric acid treatment? I use 50um free floating sections ferfused with PFA 4%. Thank you in advance for your help!! Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Qu??mica Biol??gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell??n II Piso 4 Ciudad Aut??noma de Buenos Aires C1428EGA - Argentina Tel: 54 11 4576-3300/09 - Int. 221 email:marielachertoff@gmail.com marielachertoff@qb.fcen.uba.ar ------------------------------ Message: 17 Date: Wed, 11 Mar 2015 15:53:12 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE: Masson Trichrome stain To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D508317@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Justine, I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver. As a rule of thumb, it is just easier to use plastic all the way around. The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal. That would be a stretch. As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further. This would affect the staining results. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf , histonet@lists.utsouthwestern.edu Cc: justinelanzon@hotmail.com Message-ID: <7380eaed48941.54fe3b7c@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon@hotmail.com] > > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf@gmail.com > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ ------------------------------ Message: 18 Date: Wed, 11 Mar 2015 16:04:35 +0000 From: "Marcum, Pamela A" Subject: [Histonet] RE: Old slides To: "'Mayer,Toysha N'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <8cf69427ea8f4cdf94fc08543f2d42df@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We have literally about one hundred slides to re-slip for the this reason. Are there any suggestions for large numbers of slides to be re-coverslipped as this method would be too time consuming. We have used only glass for about nine years or so and it is much better. The old ones are the problem when someone needs "THAT" slide only. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Wednesday, March 11, 2015 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Old slides Bernice, Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 19 Date: Wed, 11 Mar 2015 16:52:22 +0000 From: "Solis, Bryan" Subject: [Histonet] RE: Histonet Digest, Vol 136, Issue 12 To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <8c222bc2cfb24928808faf2d0622820a@SF1EX007.fibrogen.com> Content-Type: text/plain; charset="us-ascii" Hello, We received some liver tissue (Mouse) in 10% formalin (NFB). Then transferred to 70% ETOH. My question is that, Is it ok to transfer to 30% sucrose? So, frozen section can be perform. Please advise. Thanks, Bryan S. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 10, 2015 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 136, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Old slides. (Bernice Frederick) 2. RE: Old slides. (Jason McGough) 3. RE: Mushrooms for GMS fungus control (Morken, Timothy) 4. Re: FW: Masson's trichrome stain (John Kiernan) 5. RE: Old slides. (John Kiernan) 6. soft for microwriter (thermo scientific, Lamb, Shandon) (richard wild) 7. Re: Old slides. (b.curran.mcwilliam@gmail.com) 8. Re: Old slides. (Rene J Buesa) 9. RE: Old slides. (Gowan,Christie C) 10. IHC / Morphometry Technician wanted in Shenandoah Valley Virginia (Erin Sarricks) ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu ------------------------------ Message: 2 Date: Mon, 9 Mar 2015 14:20:09 -0600 From: Jason McGough Subject: RE: [Histonet] Old slides. To: Bernice Frederick , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=utf-8 Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough@clinlab.com www.clinlab.com -----Original message----- > From:Bernice Frederick > > Sent: Monday, March 9, 2015 1:51 PM > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Old slides. > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 3 Date: Mon, 9 Mar 2015 22:46:08 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: Mushrooms for GMS fungus control To: "koellingr@comcast.net" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF367FBA31@ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" Try this article... Acta Cytol. 2003 Nov-Dec;47(6):1043-4. Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology. da Silva VD1. Author information Abstract OBJECTIVE: To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories. STUDY DESIGN: A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears. RESULTS: Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory. CONCLUSION: It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients. PMID: 14674076 [PubMed - indexed for MEDLINE] -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Sunday, March 08, 2015 4:29 PM To: Linda Prasad (SCHN) Cc: Jeffrey Robinson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.?? Sounds like fun to me.?? I'm curious, with the emphasis now on quality control in labs??run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls??? A PI in research who??doesn't want??his paper rejected at peer review.?? A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.?? The??dot-your-i's and cross-your-t's??FDA people who might or might not OK your drug in development.?? Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. Ray, Seattle, WA ----- Original Message ----- From: "Linda Prasad (SCHN)" To: "Jeffrey Robinson" , histonet@lists.utsouthwestern.edu Sent: Sunday, March 8, 2015 4:09:02 PM Subject: [Histonet] RE: Mushrooms for GMS fungus control I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???????Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? ??Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf , histonet@lists.utsouthwestern.edu Cc: justinelanzon@hotmail.com Message-ID: <7380eaed48941.54fe3b7c@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon@hotmail.com] > > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf@gmail.com > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 5 Date: Tue, 10 Mar 2015 00:40:02 -0500 From: John Kiernan Subject: RE: [Histonet] Old slides. To: Jason McGough , Bernice Frederick , histonet@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu Message-ID: <73e09aa74b442.54fe3d62@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough@clinlab.com > > > www.clinlab.com > > ? > ? > -----Original message----- > > From:Bernice Frederick > > > > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > > > > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 6 Date: Tue, 10 Mar 2015 10:05:11 +0100 From: richard wild Subject: [Histonet] soft for microwriter (thermo scientific, Lamb, Shandon) To: histonet@lists.utsouthwestern.edu Message-ID: <54FEB3C7.40306@wanadoo.fr> Content-Type: text/plain; charset=utf-8; format=flowed Hi I am looking for labelling software (or advices) for the carousel microwriter (thermo scientific, Lamb, Shandon = same machine) (LAMB E22.01MWR) The machine is discontinuated. I would like to use serial interface rs232 and barcode scanners. Thanks for help. Richard ------------------------------ Message: 7 Date: Tue, 10 Mar 2015 11:51:25 +0000 From: b.curran.mcwilliam@gmail.com Subject: Re: [Histonet] Old slides. To: Bernice Frederick Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9FA4B5FB-11BE-47C7-AA0C-6F8416B10487@gmail.com> Content-Type: text/plain; charset=us-ascii Hi We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene & place on a slope & bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first. Bernie, St Vincent's, Dublin, Ireland > On 9 Mar 2015, at 19:41, Bernice Frederick wrote: > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 10 Mar 2015 13:43:35 +0000 (UTC) From: Rene J Buesa Subject: Re: [Histonet] Old slides. To: John Kiernan , Jason McGough , Bernice Frederick , "histonet@lists.utsouthwestern.edu" Message-ID: <1897656965.1753669.1425995015431.JavaMail.yahoo@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 To John Probably the writer is referring to slides "mounted" with the Sakura film??"coverslip".I have done it many times and the FILM will??detach easily from the section.Had it been a glass coverslip attached with, for example, Permount, acetone would not have done anything.Ren?? J.?? On Tuesday, March 10, 2015 1:40 AM, John Kiernan wrote: Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough?? wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough@clinlab.com > > > www.clinlab.com > > ?? > ?? > -----Original message----- > > From:Bernice Frederick > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 9 Mar 2015 20:01:10 +0000 From: "Gowan,Christie C" Subject: [Histonet] RE: Old slides. To: "'Bernice Frederick'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 10 Mar 2015 12:12:05 -0400 From: Erin Sarricks Subject: [Histonet] IHC / Morphometry Technician wanted in Shenandoah Valley Virginia To: histonet Message-ID: Content-Type: text/plain; charset=UTF-8 Histology Laboratory located in the Shenandoah Valley of Virginia is looking to add to it's team. In this position, you will need a working knowledge of IHC theory and practical IHC experience. The best candidate for the position will oversee immunohistochemical staining as well as perform other histology functions including trimming of specimens, paraffin embedding, microtomy and microscopic QC of slides. Experience with morphometry is preferable. Desirable candidates will possess the following: - HT (ASCP) or QIHC registration preferred - 4 years of Histology experience - 1+ years of immunohistochemistry and/or immunofluorescence experience - Keeps abreast with company's current policies and immunohistochemistry technical updates and procedures - Must be able to work independently and in a team environment Full time employment benefits include subsidized medical and dental insurance, vacation, holiday pay, and 401k after 1 year of employment. Compensation is commensurate with experience. If you are interested in this position, please respond to this post with your resume and cover letter. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 136, Issue 12 ***************************************** ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 136, Issue 13 ***************************************** From mills <@t> 3scan.com Wed Apr 1 13:40:13 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Wed Apr 1 13:40:37 2015 Subject: [Histonet] Sihler staining Message-ID: Hi Histonet, I just started working for a small start up in San Francisco called 3scan, after 18 years in clinical (human) and academia (animal tissues). We have a microscope that both sections and images at the same time. This means that my job is to get stain into whole mount tissues prior to sectioning. We found the Sihler staining technique for peripheral nerves and have been getting it to work well in animal organs that were taken directly from the animal and fixed. We are now trying embalmed human tissue, which is our final target. We had two samples, the first one came out very ragged and I don't think it had any nerve tissue left in it after the maceration. It was really hard to know where the end point is, it was so subjective, especially when looking at unfamiliar tissues. I am thinking the first sample came out so ragged because we did not put the sample into formaldehyde prior to maceration because it was embalmed and we thought that was enough. We have one more sample that is now dissected and sat in unbuffered 4% formaldehyde. *I wanted to see if anyone familiar with the technique was prepared to have a conversation with me about the end points of each stage, *We have totally read and digested all of John's suggestions from this page: http://lists.utsouthwestern.edu/mailman/htdig/histonet /2005-November/018700.html plus many others, but it would be great to talk to someone first hand. I reached out to Mu and Sanders, but they are no longer at that institution and I can't find them. Or the other person who posted on Sihler to histonet in 2005 - *Maria Mejia* maria <@t> ski.org. Their email address bounces now Is there anyone out there with first hand knowledge I can have a chat with? Thanks all, mills aka Caroline :) -- Caroline Miller Director of Histology 3Scan.com 415 2187297 From julie.hinsinger <@t> umontreal.ca Wed Apr 1 13:59:58 2015 From: julie.hinsinger <@t> umontreal.ca (Hinsinger Julie) Date: Wed Apr 1 14:00:05 2015 Subject: [Histonet] DAKO Omnis vs Ventana Ultra In-Reply-To: References: <4E1A2BAF76DB1E4CB35AA132AE0492D2201CEDB7@asphodel-cour.sim.umontreal.ca> Message-ID: <4E1A2BAF76DB1E4CB35AA132AE0492D2201CEEB4@asphodel-cour.sim.umontreal.ca> Well, Omnis can use different antibodies but problem is that manual titration can?t be done whereas it is possible on the Ultra. To run new antibodies on the Omnis, we?ll have to use a 2mL tube with a bare code, prepare a dilution for 200uL per slide, consider a dead volume of 100uL. It is less flexible in a way, but each reagent is listed and protocol set up is saved for every step. On the Ultra or Disco from Ventana, we do a 100uL dilution per slide, we open the slide tray and do our manual titration. No other information is saved in the software but it is easy to try a small volume for a starting dilution. We just had a demo with the Omnis. Very interesting but we need users feedback. Thanks a lot Tina. julie De : Tina Van Meter [mailto:tina.vanmeter@gmail.com] Envoy? : 1 avril 2015 14:30 ? : Hinsinger Julie Cc : histonet@lists.utsouthwestern.edu Objet : Re: [Histonet] DAKO Omnis vs Ventana Ultra Hi Julie, I would go with the most flexible "open" system that is capable of using a wide range of protocols. (ex. antibodies from different vendors, varied section thickness, fixed frozen sections, etc.) Kind regards, Tina Van Meter ? On Wed, Apr 1, 2015 at 1:19 PM, Hinsinger Julie > wrote: Hi, Does anyone know about some lab using DAKO Omnis for research ? If some of you tried the Omnis and the Ultra and would like to share about their own experience, it would be highly appreciated. LCS vs dynamic gap staining ? Software usage ? Costs ? Service ? Reagents and kits ? Maintenance, decontamination ? Best regards, Julie Hinsinger Histology Facility Institute for Research in Immunology and Cancer (IRIC) _Universit? de Montr?al 2950, chemin Polytechnique Pavillon Marcelle Coutu - Local 3440 H3T 1J4, Montr?al, QC (514) 343-6111 #0503 julie.hinsinger@umontreal.ca> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Apr 1 14:01:00 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Apr 1 14:01:06 2015 Subject: [Histonet] Sihler staining In-Reply-To: <7390b74836604.551c4063@uwo.ca> References: <7300e53e373ef.551c3d40@uwo.ca> <7300b37c34fa6.551c3d7d@uwo.ca> <7260bc16357ce.551c3dbb@uwo.ca> <73e0d64234a6c.551c3dfa@uwo.ca> <730082643028f.551c3e38@uwo.ca> <7350ae1136d60.551c3eb3@uwo.ca> <72a0e52337bea.551c3ef1@uwo.ca> <72b092633055d.551c3f30@uwo.ca> <7320c96e33854.551c3fe6@uwo.ca> <7350ab4f32b46.551c4025@uwo.ca> <7390b74836604.551c4063@uwo.ca> Message-ID: <7390df743628a.551bfa1c@uwo.ca> No first-hand knowledge, unfortunately, but in addition to the five references in the 2005 Histonet post that you mentioned - http://lists.utsouthwestern.edu/mailman/htdig/histonet/2005-November/018700.html - there is a more recent review with plenty of technical details and photos: Mu, L. and Sanders, I. (2010). Sihler's whole mount nerve staining technique: a review. Biotechnic & Histochemistry 85:19-42. The online version has impressive colour photos; the print version has only B&W. The corresponding author is LMU@HUMED.COM (Hackensack Univ., NJ). I don't know if he's still there. Hope this helps. John Kiernan London, Canada = = = On 01/04/15, Caroline Miller wrote: > Hi Histonet, > > I just started working for a small start up in San Francisco called 3scan, > after 18 years in clinical (human) and academia (animal tissues). We have a > microscope that both sections and images at the same time. This means that > my job is to get stain into whole mount tissues prior to sectioning. We > found the Sihler staining technique for peripheral nerves and have been > getting it to work well in animal organs that were taken directly from the > animal and fixed. > > We are now trying embalmed human tissue, which is our final target. We had > two samples, the first one came out very ragged and I don't think it had > any nerve tissue left in it after the maceration. It was really hard to > know where the end point is, it was so subjective, especially when looking > at unfamiliar tissues. I am thinking the first sample came out so ragged > because we did not put the sample into formaldehyde prior to maceration > because it was embalmed and we thought that was enough. > > We have one more sample that is now dissected and sat in unbuffered 4% > formaldehyde. *I wanted to see if anyone familiar with the technique was > prepared to have a conversation with me about the end points of each stage, > *We have totally read and digested all of John's suggestions from this page: > http://lists.utsouthwestern.edu/mailman/htdig/histonet//2005-November/018700.html > plus many others, but it would be great to talk to someone first hand. > > I reached out to Mu and Sanders, but they are no longer at that institution > and I can't find them. Or the other person who posted on Sihler to histonet > in 2005 - *Maria Mejia* maria <@t> ski.org. Their email address bounces > now > > > Is there anyone out there with first hand knowledge I can have a chat with? > > Thanks all, > > mills aka Caroline :) > > > -- > Caroline Miller > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From linda.prasad <@t> health.nsw.gov.au Wed Apr 1 17:39:00 2015 From: linda.prasad <@t> health.nsw.gov.au (Linda Prasad (SCHN)) Date: Wed Apr 1 17:39:21 2015 Subject: [Histonet] Allowable temperature range In-Reply-To: References: Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E88FF5@xmdb03.nch.kids> If it's in formalin it can just stay at room temperature. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim H Sent: Thursday, 2 April 2015 1:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Allowable temperature range What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage? Any ideas? Tim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From patpxs <@t> gmail.com Wed Apr 1 18:12:22 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Wed Apr 1 18:12:25 2015 Subject: [Histonet] Positive Control for IF? Message-ID: Good Afternoon Netters, Since we went down the path of hot dogs and Slim Jim's as positive controls for FFPE stains, I was wondering..... Is there a source, like a hot dog or piece of steak, that could be a positive control for immunofluorescence C4d? What I'd really like to find is some food or food product (our positive patient biopsies for the frozen IF are teeny-tiny) that is positive for the whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the picture. Please send your suggestions my way. Thanks in advance, Paula :-) From patpxs <@t> gmail.com Wed Apr 1 18:14:16 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Wed Apr 1 18:14:19 2015 Subject: [Histonet] C3d? Message-ID: Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) From marktarango <@t> gmail.com Wed Apr 1 18:16:54 2015 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Apr 1 18:17:17 2015 Subject: [Histonet] Positive Control for IF? In-Reply-To: References: Message-ID: It is April 1st, isn't it? On Wed, Apr 1, 2015 at 4:12 PM, Paula Sicurello wrote: > Good Afternoon Netters, > > Since we went down the path of hot dogs and Slim Jim's as positive controls > for FFPE stains, I was wondering..... > > Is there a source, like a hot dog or piece of steak, that could be a > positive control for immunofluorescence C4d? > > What I'd really like to find is some food or food product (our positive > patient biopsies for the frozen IF are teeny-tiny) that is positive for the > whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the > picture. > > Please send your suggestions my way. > > Thanks in advance, > > Paula :-) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jburch01 <@t> gmail.com Wed Apr 1 20:16:29 2015 From: jburch01 <@t> gmail.com (Jim Burchette) Date: Wed Apr 1 20:16:34 2015 Subject: [Histonet] Positive Control for IF? In-Reply-To: References: Message-ID: Paula, does your institution perform autopsies? If so, have the staff notify you when there is a lupus nephritis case. Have them send you fresh renal cortex. Cut up the tissue into small cubes and freeze for later use as IF control. Run your battery of Ig's, compliment and light chains. Most should have some reactivity. You might be able to use a rejected kidney for C4d if it isn't to necrotic. Transplant heart and liver can also be used for rejection antibodies. JB On Wednesday, April 1, 2015, Paula Sicurello wrote: > Good Afternoon Netters, > > Since we went down the path of hot dogs and Slim Jim's as positive controls > for FFPE stains, I was wondering..... > > Is there a source, like a hot dog or piece of steak, that could be a > positive control for immunofluorescence C4d? > > What I'd really like to find is some food or food product (our positive > patient biopsies for the frozen IF are teeny-tiny) that is positive for the > whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the > picture. > > Please send your suggestions my way. > > Thanks in advance, > > Paula :-) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jim Burchette "Fly Fishing Bum" *<'(((><* From LSebree <@t> uwhealth.org Thu Apr 2 07:25:05 2015 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Apr 2 07:25:09 2015 Subject: [Histonet] C3d? In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF121997@UWHC-MBX12.uwhis.hosp.wisc.edu> Cleveland Clinic does it by IHC and I think also by IF. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, April 01, 2015 6:14 PM To: HistoNet Subject: [Histonet] C3d? Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Thu Apr 2 09:13:49 2015 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Apr 2 09:13:55 2015 Subject: [Histonet] interface between Bond and Cerner CoPath Plus Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3601159C5EFA@PEITHA.wad.pa-ucl.com> Does anyone currently have an interface between the Leica Bond stainer and Cerner CoPath plus? How does it work? Cost? Thank you Nancy Schmitt MLT, HT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From DSiena <@t> statlab.com Thu Apr 2 11:00:25 2015 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu Apr 2 11:00:36 2015 Subject: [Histonet] Question about Formic acid decal and TRAP stain Message-ID: Hi Histonetters, I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help. Debbie Siena dsiena@statlab.com | www.statlab.com From liz <@t> premierlab.com Thu Apr 2 11:10:11 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 2 11:10:17 2015 Subject: [Histonet] RE: Question about Formic acid decal and TRAP stain In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79F17FFC@SBS2K8.premierlab.local> Debbie In our hands it has not worked on formic acid decaled samples, EDTA decal only. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Thursday, April 02, 2015 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Formic acid decal and TRAP stain Hi Histonetters, I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help. Debbie Siena dsiena@statlab.com | www.statlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MCHUGHK3 <@t> ccf.org Thu Apr 2 12:11:49 2015 From: MCHUGHK3 <@t> ccf.org (McHugh, Kelsey) Date: Thu Apr 2 12:11:54 2015 Subject: [Histonet] FW: Paraffin Waste Disposal In-Reply-To: References: Message-ID: Hello All, I know each state's EPA laws regulate what can be done with the waste paraffin generated during embedding and processing, but I was wondering: how do you dispose of your paraffin waste? Ours is thrown away in biohazard bags and incinerated, though by our state law (OH), paraffin with fixed tissue can be thrown in the regular trash as long as no additives qualify it as a hazardous waste. We are performing a cost analysis and investigating the more "green" options that exist for paraffin disposal/recycling - I thought a poll of all you histo lab experts would be a great starting point. Thank you for your help! -Kelsey =================================== Please consider the environment before printing this e-mail Cleveland Clinic is ranked as one of the top hospitals in America by U.S.News & World Report (2014). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From koellingr <@t> comcast.net Thu Apr 2 12:16:13 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Apr 2 12:16:32 2015 Subject: [Histonet] Question about Formic acid decal and TRAP stain In-Reply-To: References: Message-ID: <1825621960.18197278.1427994973007.JavaMail.zimbra@comcast.net> Hi Debra, My experience differs from Elizabeth and others.? Indeed have made thousands of mouse bone preparations with formic acid decaled sections.? Here is an article that didn't copy paste so well: ? RANK is the intrinsic hematopoietic cell surface receptor that controls osteoclastogenesis and regulation of bone mass and calcium metabolism Ji Li*, Ildiko Sarosi ? , Xiao-Qiang Yan ? , Sean Morony ? , Casey Capparelli ? , Hong-Lin Tan ? , Susan McCabe*, Robin Elliott*, Sheila Scully ?Gwyneth Van ? , Stephen Kaufman ? , Shao-Chieh Juan ? , Yu Sun ? , John Tarpley ? , Laura Martin ? , Kathleen Christensen ?James McCabe ? , Paul Kostenuik ? , Hailing Hsu*, Frederick Fletcher ? , Colin R. Dunstan ? , David L. Lacey , and William J. Boyle* Departments of *Cell Biology and Pathology, Amgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320 Edited by David V. Goeddel, Tularik, Inc., South San Francisco, CA, and approved December 20, 1999 (received for review September 30, 1999) ? PNAS u February 15, 2000 u vol. 97 u no. 4 u 1571 ? Is in PNAS and while the methods are not here in this article, look in the reference section to materials and methods used and see beautiful pictures of TRAP?from formic acid decaled bones.? I did them for years after that.? Complete fixation and gentle formic acid (or immunocal) and indeed this can be done. ? Ray (retired in Lake Forest Park, WA, golf and science education outreach for K-12 busy) ? ----- Original Message ----- From: "Debra Siena" To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2015 9:00:25 AM Subject: [Histonet] Question about Formic acid decal and TRAP stain Hi Histonetters, I have a question to ask if you don't mind. ?Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? ?If anyone has any experience or references that they could point me to, I would greatly appreciate it. ?Thanks in advance for your help. Debbie Siena dsiena@statlab.com | www.statlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Thu Apr 2 12:16:40 2015 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Thu Apr 2 12:16:43 2015 Subject: [Histonet] Deb - TRAP stain Message-ID: <551D7978.10908@svm.vetmed.wisc.edu> I agree with Liz. EDTA. -- Vicki L. Kalscheur School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 2015 Linden Drive Madison, WI 53706-1100 Phone: 608-262-8534 kalschev@vetmed.wisc.edu From Sarah_Mack <@t> urmc.rochester.edu Thu Apr 2 12:21:21 2015 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Thu Apr 2 12:22:32 2015 Subject: [Histonet] RE:Question about Formic acid decal and TRAP stain In-Reply-To: <201504021702.t32H2L7V027237@pps.reinject> References: <201504021702.t32H2L7V027237@pps.reinject> Message-ID: In our hands we have never had TRAP success on Formic acid decalcified tissue. Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, April 02, 2015 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 137, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Allowable temperature range (Linda Prasad (SCHN)) 2. Positive Control for IF? (Paula Sicurello) 3. C3d? (Paula Sicurello) 4. Re: Positive Control for IF? (Mark Tarango) 5. Re: Positive Control for IF? (Jim Burchette) 6. RE: C3d? (Sebree Linda A) 7. interface between Bond and Cerner CoPath Plus (Nancy Schmitt) 8. Question about Formic acid decal and TRAP stain (Debra Siena) 9. RE: Question about Formic acid decal and TRAP stain (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Wed, 1 Apr 2015 22:39:00 +0000 From: "Linda Prasad (SCHN)" Subject: RE: [Histonet] Allowable temperature range To: "'Tim H'" , "histonet@lists.utsouthwestern.edu" Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E88FF5@xmdb03.nch.kids> Content-Type: text/plain; charset="utf-8" If it's in formalin it can just stay at room temperature. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.schn.health.nsw.gov.au&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=9Qb6_bEDCJKheqNhwxTb8wBpygzmxDAIDXa9byoCD2k&e= Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ? Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim H Sent: Thursday, 2 April 2015 1:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Allowable temperature range What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage? Any ideas? Tim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 2 Date: Wed, 1 Apr 2015 16:12:22 -0700 From: Paula Sicurello Subject: [Histonet] Positive Control for IF? To: HistoNet Message-ID: Content-Type: text/plain; charset=UTF-8 Good Afternoon Netters, Since we went down the path of hot dogs and Slim Jim's as positive controls for FFPE stains, I was wondering..... Is there a source, like a hot dog or piece of steak, that could be a positive control for immunofluorescence C4d? What I'd really like to find is some food or food product (our positive patient biopsies for the frozen IF are teeny-tiny) that is positive for the whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the picture. Please send your suggestions my way. Thanks in advance, Paula :-) ------------------------------ Message: 3 Date: Wed, 1 Apr 2015 16:14:16 -0700 From: Paula Sicurello Subject: [Histonet] C3d? To: HistoNet Message-ID: Content-Type: text/plain; charset=UTF-8 Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) ------------------------------ Message: 4 Date: Wed, 1 Apr 2015 16:16:54 -0700 From: Mark Tarango Subject: Re: [Histonet] Positive Control for IF? To: Paula Sicurello Cc: HistoNet Message-ID: Content-Type: text/plain; charset=UTF-8 It is April 1st, isn't it? On Wed, Apr 1, 2015 at 4:12 PM, Paula Sicurello wrote: > Good Afternoon Netters, > > Since we went down the path of hot dogs and Slim Jim's as positive controls > for FFPE stains, I was wondering..... > > Is there a source, like a hot dog or piece of steak, that could be a > positive control for immunofluorescence C4d? > > What I'd really like to find is some food or food product (our positive > patient biopsies for the frozen IF are teeny-tiny) that is positive for the > whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the > picture. > > Please send your suggestions my way. > > Thanks in advance, > > Paula :-) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= > ------------------------------ Message: 5 Date: Wed, 1 Apr 2015 20:16:29 -0500 From: Jim Burchette Subject: Re: [Histonet] Positive Control for IF? To: Paula Sicurello Cc: HistoNet Message-ID: Content-Type: text/plain; charset=UTF-8 Paula, does your institution perform autopsies? If so, have the staff notify you when there is a lupus nephritis case. Have them send you fresh renal cortex. Cut up the tissue into small cubes and freeze for later use as IF control. Run your battery of Ig's, compliment and light chains. Most should have some reactivity. You might be able to use a rejected kidney for C4d if it isn't to necrotic. Transplant heart and liver can also be used for rejection antibodies. JB On Wednesday, April 1, 2015, Paula Sicurello wrote: > Good Afternoon Netters, > > Since we went down the path of hot dogs and Slim Jim's as positive controls > for FFPE stains, I was wondering..... > > Is there a source, like a hot dog or piece of steak, that could be a > positive control for immunofluorescence C4d? > > What I'd really like to find is some food or food product (our positive > patient biopsies for the frozen IF are teeny-tiny) that is positive for the > whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the > picture. > > Please send your suggestions my way. > > Thanks in advance, > > Paula :-) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= > -- Jim Burchette "Fly Fishing Bum" *<'(((><* ------------------------------ Message: 6 Date: Thu, 2 Apr 2015 12:25:05 +0000 From: Sebree Linda A Subject: RE: [Histonet] C3d? To: 'Paula Sicurello' , HistoNet Message-ID: <77DD817201982748BC67D7960F2F76AF121997@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="utf-8" Cleveland Clinic does it by IHC and I think also by IF. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, April 01, 2015 6:14 PM To: HistoNet Subject: [Histonet] C3d? Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= ------------------------------ Message: 7 Date: Thu, 2 Apr 2015 14:13:49 +0000 From: Nancy Schmitt Subject: [Histonet] interface between Bond and Cerner CoPath Plus To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3601159C5EFA@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Does anyone currently have an interface between the Leica Bond stainer and Cerner CoPath plus? How does it work? Cost? Thank you Nancy Schmitt MLT, HT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 8 Date: Thu, 2 Apr 2015 16:00:25 +0000 From: Debra Siena Subject: [Histonet] Question about Formic acid decal and TRAP stain To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetters, I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help. Debbie Siena dsiena@statlab.com | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= ------------------------------ Message: 9 Date: Thu, 2 Apr 2015 10:10:11 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: Question about Formic acid decal and TRAP stain To: Debra Siena , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79F17FFC@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Debbie In our hands it has not worked on formic acid decaled samples, EDTA decal only. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com https://urldefense.proofpoint.com/v2/url?u=http-3A__www.premierlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=F3th4LJ6QUaggfgVvpdB5XB0_s-ZkCp_YEJKag4d-Ic&e= March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Thursday, April 02, 2015 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Formic acid decal and TRAP stain Hi Histonetters, I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help. Debbie Siena dsiena@statlab.com | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= End of Histonet Digest, Vol 137, Issue 3 **************************************** From koellingr <@t> comcast.net Thu Apr 2 12:35:19 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Apr 2 12:35:35 2015 Subject: [Histonet] RE:Question about Formic acid decal and TRAP stain In-Reply-To: References: <201504021702.t32H2L7V027237@pps.reinject> Message-ID: <1772627974.18218273.1427996119706.JavaMail.zimbra@comcast.net> Debra, it appears most of the histology world disagrees with me but I stand by my post.? If TRAP didn't work with formic acid in our hands, a major pharmaceutical treatment wouldn't be ready to help women with post-menopausal osteoporosis. Ray ----- Original Message ----- From: "Sarah Mack" To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2015 10:21:21 AM Subject: [Histonet] RE:Question about Formic acid decal and TRAP stain In our hands we have never had TRAP success on Formic acid decalcified tissue. Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Thursday, April 02, 2015 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 137, Issue 3 Send Histonet mailing list submissions to ?? ? ? ?histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ?? ? ? ?https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= or, via email, send a message with subject or body 'help' to ?? ? ? ?histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ?? ? ? ?histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ?? 1. RE: Allowable temperature range (Linda Prasad (SCHN)) ?? 2. Positive Control for IF? (Paula Sicurello) ?? 3. C3d? (Paula Sicurello) ?? 4. Re: Positive Control for IF? (Mark Tarango) ?? 5. Re: Positive Control for IF? (Jim Burchette) ?? 6. RE: C3d? (Sebree Linda A) ?? 7. interface between Bond and Cerner CoPath Plus (Nancy Schmitt) ?? 8. Question about Formic acid decal and TRAP stain (Debra Siena) ?? 9. RE: Question about Formic acid decal and TRAP stain ?? ? ?(Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Wed, 1 Apr 2015 22:39:00 +0000 From: "Linda Prasad (SCHN)" Subject: RE: [Histonet] Allowable temperature range To: "'Tim H'" , ?? ? ? ?"histonet@lists.utsouthwestern.edu" ?? ? ? ? Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E88FF5@xmdb03.nch.kids> Content-Type: text/plain; charset="utf-8" If it's in formalin it can just stay at room temperature. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.schn.health.nsw.gov.au&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=9Qb6_bEDCJKheqNhwxTb8wBpygzmxDAIDXa9byoCD2k&e= Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ? ?Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim H Sent: Thursday, 2 April 2015 1:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Allowable temperature range What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage? Any ideas? ?Tim ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 2 Date: Wed, 1 Apr 2015 16:12:22 -0700 From: Paula Sicurello Subject: [Histonet] Positive Control for IF? To: HistoNet Message-ID: ?? ? ? ? Content-Type: text/plain; charset=UTF-8 Good Afternoon Netters, Since we went down the path of hot dogs and Slim Jim's as positive controls for FFPE stains, I was wondering..... Is there a source, like a hot dog or piece of steak, that could be a positive control for immunofluorescence C4d? What I'd really like to find is some food or food product (our positive patient biopsies for the frozen IF are teeny-tiny) that is positive for the whole host of IF stains: ?IgG, IgA, IgM, Kappa, Lambda, you get the picture. Please send your suggestions my way. Thanks in advance, Paula ?:-) ------------------------------ Message: 3 Date: Wed, 1 Apr 2015 16:14:16 -0700 From: Paula Sicurello Subject: [Histonet] C3d? To: HistoNet Message-ID: ?? ? ? ? Content-Type: text/plain; charset=UTF-8 Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula ?:-) ------------------------------ Message: 4 Date: Wed, 1 Apr 2015 16:16:54 -0700 From: Mark Tarango Subject: Re: [Histonet] Positive Control for IF? To: Paula Sicurello Cc: HistoNet Message-ID: ?? ? ? ? Content-Type: text/plain; charset=UTF-8 It is April 1st, isn't it? On Wed, Apr 1, 2015 at 4:12 PM, Paula Sicurello wrote: > Good Afternoon Netters, > > Since we went down the path of hot dogs and Slim Jim's as positive controls > for FFPE stains, I was wondering..... > > Is there a source, like a hot dog or piece of steak, that could be a > positive control for immunofluorescence C4d? > > What I'd really like to find is some food or food product (our positive > patient biopsies for the frozen IF are teeny-tiny) that is positive for the > whole host of IF stains: ?IgG, IgA, IgM, Kappa, Lambda, you get the > picture. > > Please send your suggestions my way. > > Thanks in advance, > > Paula ?:-) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= > ------------------------------ Message: 5 Date: Wed, 1 Apr 2015 20:16:29 -0500 From: Jim Burchette Subject: Re: [Histonet] Positive Control for IF? To: Paula Sicurello Cc: HistoNet Message-ID: ?? ? ? ? Content-Type: text/plain; charset=UTF-8 Paula, does your institution perform autopsies? If so, have the staff notify you when there is a lupus nephritis case. Have them send you fresh renal cortex. Cut up the tissue into small cubes and freeze for later use as IF control. Run your battery of Ig's, compliment and light chains. Most should have some reactivity. You might be able to use a rejected kidney for C4d if it isn't to necrotic. Transplant heart and liver can also be used for rejection antibodies. JB On Wednesday, April 1, 2015, Paula Sicurello wrote: > Good Afternoon Netters, > > Since we went down the path of hot dogs and Slim Jim's as positive controls > for FFPE stains, I was wondering..... > > Is there a source, like a hot dog or piece of steak, that could be a > positive control for immunofluorescence C4d? > > What I'd really like to find is some food or food product (our positive > patient biopsies for the frozen IF are teeny-tiny) that is positive for the > whole host of IF stains: ?IgG, IgA, IgM, Kappa, Lambda, you get the > picture. > > Please send your suggestions my way. > > Thanks in advance, > > Paula ?:-) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= > -- Jim Burchette "Fly Fishing Bum" ?*<'(((><* ------------------------------ Message: 6 Date: Thu, 2 Apr 2015 12:25:05 +0000 From: Sebree Linda A Subject: RE: [Histonet] C3d? To: 'Paula Sicurello' , HistoNet ?? ? ? ? Message-ID: ?? ? ? ?<77DD817201982748BC67D7960F2F76AF121997@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="utf-8" Cleveland Clinic does it by IHC and I think also by IF. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, April 01, 2015 6:14 PM To: HistoNet Subject: [Histonet] C3d? Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula ?:-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= ------------------------------ Message: 7 Date: Thu, 2 Apr 2015 14:13:49 +0000 From: Nancy Schmitt Subject: [Histonet] interface between Bond and Cerner CoPath Plus To: "'histonet@lists.utsouthwestern.edu'" ?? ? ? ? Message-ID: ?? ? ? ?<906B4DA90ED1DB4DB6C7E94D7CEE6C3601159C5EFA@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Does anyone currently have an interface between the Leica Bond stainer and Cerner CoPath plus? ?How does it work? Cost? Thank you Nancy Schmitt MLT, HT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 8 Date: Thu, 2 Apr 2015 16:00:25 +0000 From: Debra Siena Subject: [Histonet] Question about Formic acid decal and TRAP stain To: "histonet@lists.utsouthwestern.edu" ?? ? ? ? Message-ID: ?? ? ? ? Content-Type: text/plain; charset="us-ascii" Hi Histonetters, I have a question to ask if you don't mind. ?Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? ?If anyone has any experience or references that they could point me to, I would greatly appreciate it. ?Thanks in advance for your help. Debbie Siena dsiena@statlab.com | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= ------------------------------ Message: 9 Date: Thu, 2 Apr 2015 10:10:11 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: Question about Formic acid decal and TRAP ?? ? ? ?stain To: Debra Siena , ?? ? ? ?"histonet@lists.utsouthwestern.edu" ?? ? ? ? Message-ID: ?? ? ? ?<14E2C6176416974295479C64A11CB9AE019C79F17FFC@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Debbie In our hands it has not worked on formic acid decaled samples, EDTA decal only. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com https://urldefense.proofpoint.com/v2/url?u=http-3A__www.premierlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=F3th4LJ6QUaggfgVvpdB5XB0_s-ZkCp_YEJKag4d-Ic&e= March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Thursday, April 02, 2015 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Formic acid decal and TRAP stain Hi Histonetters, I have a question to ask if you don't mind. ?Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? ?If anyone has any experience or references that they could point me to, I would greatly appreciate it. ?Thanks in advance for your help. Debbie Siena dsiena@statlab.com | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e= End of Histonet Digest, Vol 137, Issue 3 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fourfonners <@t> yahoo.com Thu Apr 2 12:47:30 2015 From: fourfonners <@t> yahoo.com (Sheila Fonner) Date: Thu Apr 2 12:47:33 2015 Subject: [Histonet] Need Antibody Message-ID: <942781803.3937180.1427996850243.JavaMail.yahoo@mail.yahoo.com> Good afternoon Histonetters! Does anyone out there know of a good antibody for Bartonella? I would appreciate any information you could give me. Thank you for your knowledge and willingness to share information. SheilaKnoxville, TN From JRobinson <@t> pathology-associates.com Thu Apr 2 13:39:49 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Thu Apr 2 13:39:55 2015 Subject: [Histonet] Need Antibody In-Reply-To: <942781803.3937180.1427996850243.JavaMail.yahoo@mail.yahoo.com> References: <942781803.3937180.1427996850243.JavaMail.yahoo@mail.yahoo.com> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8F0CE@PAEXCH1.PathologyAssociates.local> Hi Sheila- I received some information from BioCare last year that they were releasing a Bartonella (ASR) antibody. I just checked their website and I did not see it listed but you might want to check with them (www.biocare.net). Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Thursday, April 02, 2015 10:48 AM To: Histonet Subject: [Histonet] Need Antibody Good afternoon Histonetters! Does anyone out there know of a good antibody for Bartonella? I would appreciate any information you could give me. Thank you for your knowledge and willingness to share information. SheilaKnoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From Cnituda <@t> nvdermatology.com Thu Apr 2 13:42:39 2015 From: Cnituda <@t> nvdermatology.com (Carl Nituda) Date: Thu Apr 2 13:42:48 2015 Subject: [Histonet] Need Antibody In-Reply-To: <942781803.3937180.1427996850243.JavaMail.yahoo@mail.yahoo.com> References: <942781803.3937180.1427996850243.JavaMail.yahoo@mail.yahoo.com> Message-ID: http://www.novusbio.com/Bartonella-henselae-Antibody-H2A10_NB600-1208.html CN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Thursday, April 02, 2015 10:48 AM To: Histonet Subject: [Histonet] Need Antibody Good afternoon Histonetters! Does anyone out there know of a good antibody for Bartonella? I would appreciate any information you could give me. Thank you for your knowledge and willingness to share information. SheilaKnoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From halsteaj <@t> ohsu.edu Thu Apr 2 13:47:44 2015 From: halsteaj <@t> ohsu.edu (Jeff Halstead) Date: Thu Apr 2 13:47:52 2015 Subject: [Histonet] cleaning glassware Message-ID: <398DA4E9BECCE44288904391038A80DE2FF4E464@EXMB03.ohsu.edu> Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx From JRobinson <@t> pathology-associates.com Thu Apr 2 14:00:10 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Thu Apr 2 14:00:22 2015 Subject: FW: [Histonet] Need Antibody (Bartonella/ Cat-Scratch) update Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8F102@PAEXCH1.PathologyAssociates.local> Hi Sheila- I received a reply from BioCare so I am passing the information along. Jeff. -----Original Message----- From: Dax Arguello [mailto:darguello@biocare.net] Sent: Thursday, April 02, 2015 11:46 AM To: Jeffrey Robinson Subject: RE: [Histonet] Need Antibody Jeff, We do have the Bartonella Henselea antibody listed under Cat Scratch if you wanted to pass that info on to Sheila. It is now IVD as well. http://biocare.net/product/cat-scratch-antibody/ Best regards, Dax Arguello Account Executive Biocare Medical, LLC Mobile: (925) 586-2591 Fax: (925) 603-8080 E-mail: darguello@biocare.net www.biocare.net ?4040 Pike Lane?Concord, CA 94520?(800) 799-9499 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, April 02, 2015 11:40 AM To: Sheila Fonner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Need Antibody Hi Sheila- I received some information from BioCare last year that they were releasing a Bartonella (ASR) antibody. I just checked their website and I did not see it listed but you might want to check with them (www.biocare.net). Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Thursday, April 02, 2015 10:48 AM To: Histonet Subject: [Histonet] Need Antibody Good afternoon Histonetters! Does anyone out there know of a good antibody for Bartonella? I would appreciate any information you could give me. Thank you for your knowledge and willingness to share information. SheilaKnoxville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From amurvosh <@t> advancederm.net Thu Apr 2 14:01:30 2015 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Thu Apr 2 14:01:39 2015 Subject: [Histonet] RE: cleaning glassware In-Reply-To: <398DA4E9BECCE44288904391038A80DE2FF4E464@EXMB03.ohsu.edu> References: <398DA4E9BECCE44288904391038A80DE2FF4E464@EXMB03.ohsu.edu> Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7A81528@Exchange.Advancederm.net> I always used to rinse the containers I used with alcohol and let them dry before doing the stain. Some people use an acid alcohol rinse. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Apr 2 14:35:13 2015 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Apr 2 14:35:32 2015 Subject: [Histonet] RE: cleaning glassware In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7A81528@Exchange.Advancederm.net> References: <398DA4E9BECCE44288904391038A80DE2FF4E464@EXMB03.ohsu.edu>, <22BDD9AABC13E24E95D1CF064B75C4B7A81528@Exchange.Advancederm.net> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53E7FCA@xmdb04.nch.kids> Also check the gelatine - is it a new batch? ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurvosh@advancederm.net] Sent: Friday, 3 April 2015 6:01 AM To: Jeff Halstead; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cleaning glassware I always used to rinse the containers I used with alcohol and let them dry before doing the stain. Some people use an acid alcohol rinse. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From rjr6 <@t> psu.edu Thu Apr 2 14:36:20 2015 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Thu Apr 2 14:36:25 2015 Subject: [Histonet] RE: cleaning glassware In-Reply-To: <398DA4E9BECCE44288904391038A80DE2FF4E464@EXMB03.ohsu.edu> References: <398DA4E9BECCE44288904391038A80DE2FF4E464@EXMB03.ohsu.edu> Message-ID: I work in a small veterinary lab and when I do the Warthin Starry stain I mix all the reagents in never used before disposable plastic beakers and I stain the slides in an un-used slide mailer. Roberta Horner Animal Diagnostic Lab Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Apr 2 14:37:55 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 2 14:37:59 2015 Subject: [Histonet] RE: cleaning glassware In-Reply-To: References: <398DA4E9BECCE44288904391038A80DE2FF4E464@EXMB03.ohsu.edu> Message-ID: We always acid clean before doing any silver stain. No metal !!!! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, April 02, 2015 2:36 PM To: Jeff Halstead; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cleaning glassware I work in a small veterinary lab and when I do the Warthin Starry stain I mix all the reagents in never used before disposable plastic beakers and I stain the slides in an un-used slide mailer. Roberta Horner Animal Diagnostic Lab Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Apr 2 15:22:01 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Thu Apr 2 15:22:05 2015 Subject: [Histonet] C3d? In-Reply-To: <77DD817201982748BC67D7960F2F76AF121997@UWHC-MBX12.uwhis.hosp.wisc.edu> References: , <77DD817201982748BC67D7960F2F76AF121997@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Indirect IF Joelle Weaver MAOM, HTL (ASCP) QIHC From: LSebree@uwhealth.org To: patpxs@gmail.com; histonet@lists.utsouthwestern.edu Date: Thu, 2 Apr 2015 12:25:05 +0000 Subject: RE: [Histonet] C3d? CC: Cleveland Clinic does it by IHC and I think also by IF. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, April 01, 2015 6:14 PM To: HistoNet Subject: [Histonet] C3d? Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Apr 2 17:12:55 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Apr 2 17:13:17 2015 Subject: [Histonet] Re: TRAP staining on formic acid decalcified bone reference Message-ID: <000801d06d92$2b8f2a80$82ad7f80$@bresnan.net> The reference within a reference from Ray is A Chimeric Form of Osteoprotegerin Inhibits Hypercalcemia and Bone Resorption Induced by IL-1?, TNF-?, PTH, PTHrP, and 1,25(OH)2D3 . Sean Morony et al . J Bone Mineral Res V 14, pp 1478-1485. However, the formic acid decalcification method is not described in detail and merely says "formic acid" but whether this is buffered formic acid or just dilute formic acid in water only is not stated. Ray might elaborate on what specific formic acid recipe he used as many in research don't always use buffered formic acid decalcifiying solutions. I would assume Morony et all used a buffered formic acid with either sodium formate or sodium citrate and controlled so as to not overexpose TRAP to acids longer than necessary. One publication, i.e., Eggert and Germain. Stable Acid Phosphatase I. Demonstration and Distribution. Histochem 66, pp 301-317, 1980) discussed in detail the rapid demineralization in acidic buffers i.e. buffered formic acid for staining of stable forms of acid phosphatase. I have both of these publications on file and will forward privately. I would err on the side of using a buffered formic acid with either sodium formate or sodium citrate for doing this and use decalcification endpoint testing to avoid over exposure to acid i.e. over decalcification. Take care Gayle M. Callis HTL/HT/MT(ASCP) From koellingr <@t> comcast.net Thu Apr 2 18:37:16 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Apr 2 18:37:31 2015 Subject: [Histonet] Re: TRAP staining on formic acid decalcified bone reference In-Reply-To: <000801d06d92$2b8f2a80$82ad7f80$@bresnan.net> References: <000801d06d92$2b8f2a80$82ad7f80$@bresnan.net> Message-ID: <1112580921.18453898.1428017836220.JavaMail.zimbra@comcast.net> Hi all, as usual Gayle was right on.? Use a buffered (more gentle) formic acid; not just formic acid per se of any water diluted concentration.? For end point testing, critical, we used a radiograph machine instead of chemical endpoints which?is also?fine;we just had access to a lot of equipment. Ray Washington ----- Original Message ----- From: "Gayle Callis" To: "Histonet" Sent: Thursday, April 2, 2015 3:12:55 PM Subject: [Histonet] Re: TRAP staining on formic acid decalcified bone????????reference The reference within a reference from Ray is A Chimeric Form of Osteoprotegerin Inhibits Hypercalcemia and Bone Resorption Induced by IL-1?, TNF-?, PTH, PTHrP, and 1,25(OH)2D3 . ? Sean Morony et al . ?J Bone Mineral Res V 14, pp 1478-1485. ? ? However, the formic acid decalcification method is not described in detail and merely says "formic acid" but whether this is buffered formic acid or just dilute formic acid in water only is not stated. ? ? Ray might elaborate on what specific formic acid recipe he used as many in research don't always use buffered formic acid decalcifiying solutions. ? ? I would assume Morony et all used a buffered formic acid with either sodium formate or sodium citrate and controlled so as to not overexpose TRAP to acids longer than necessary. ? One publication, ?i.e., ?Eggert and Germain. Stable Acid Phosphatase I. Demonstration and Distribution. ?Histochem 66, pp 301-317, 1980) discussed in detail the ?rapid demineralization in acidic buffers i.e. buffered formic acid for staining of stable forms of acid phosphatase. ? ? I have both of these publications on file and will forward privately. ? ? I would err on the side of using a buffered formic acid with either sodium formate or sodium citrate for doing this and use decalcification endpoint testing to avoid over exposure to acid i.e. over decalcification. ? ? ? Take care ? Gayle M. Callis HTL/HT/MT(ASCP) ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Thu Apr 2 22:15:35 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Apr 2 22:15:38 2015 Subject: [Histonet] Another question: C4d and preeclamptic placentas Message-ID: Hello Netters, plus Matt and Keith ;-) I'm picking the collective brain of the Histonet... I've found some literature that suggests that up to 50% of preeclamptic placentas that resulted in a negative outcome have diffuse C4d staining when using IHC. Has anyone tried C4d IHC or IF on preeclamptic placentas? Tomorrow's Friday....so have a nice weekend everyone! Paula :-) From jshelley <@t> sanfordburnham.org Fri Apr 3 08:01:10 2015 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Fri Apr 3 08:01:15 2015 Subject: [Histonet] Florida Society for Histotechnology 2015 Spring Meeting Message-ID: Good Morning! I wanted to let everyone know that the FSH 2015 Meeting Program is complete and we are looking forward to a great meeting on May 14-17, 2015 at the Lake Buena Vista Palace Hotel and Spa. We especially invite those here in the State of Florida to come in order to fulfill your CEU requirements for both your state licensure and for you HT/HTL certification. We are also extending the invitation to those of you outside the state if you are looking to get away from the winter and would like the wonderful bright sun and vacation atmosphere of our location. Our meeting will be nestled within the Disney area and would be a great opportunity for learning and relaxation. I have included some key links to be able to make your plans that much easier. I look forward to seeing you at the meeting. Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402&hotelID=6579 Need to book no later than 4-23-15 Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155&lbrd=1&rtypeid=380141 Sincerely, John Shelley 2014-16 FSH President From halsteaj <@t> ohsu.edu Fri Apr 3 11:36:15 2015 From: halsteaj <@t> ohsu.edu (Jeff Halstead) Date: Fri Apr 3 11:36:21 2015 Subject: [Histonet] cleaning glassware Message-ID: <398DA4E9BECCE44288904391038A80DE2FF4E526@EXMB03.ohsu.edu> Hi All-thankyou for the tips-will investigate each and report thanx again From rosenfeldtek <@t> hotmail.com Fri Apr 3 13:37:40 2015 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Fri Apr 3 13:37:46 2015 Subject: [Histonet] Kidney Stone Histology? Message-ID: How the heck do I process and section kidney stones? And what kind of stain do you like for them? Thanks Jerry Ricks Research Scientist University of Washington Department of Pathology From sally.norton <@t> seattlechildrens.org Fri Apr 3 13:42:09 2015 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Fri Apr 3 13:42:16 2015 Subject: [Histonet] Kidney Stone Histology? In-Reply-To: References: Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.sea.kids> The only thing we do with stones is send them to the Mayo clinic for chemical analysis. Sally Norton Seattle Children's -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Friday, April 03, 2015 11:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Stone Histology? How the heck do I process and section kidney stones? And what kind of stain do you like for them? Thanks Jerry Ricks Research Scientist University of Washington Department of Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mucram11 <@t> comcast.net Fri Apr 3 13:46:03 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri Apr 3 13:46:19 2015 Subject: [Histonet] Kidney Stone Histology? In-Reply-To: <1357F84B33D39A46BA015A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.sea.kids> References: <1357F84B33D39A46BA015A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.sea.kids> Message-ID: <1966901990.6213413.1428086763800.JavaMail.zimbra@comcast.net> Same here although we don't use Mayo.? They are gross only and ship or store. ? Pam Marcum UAMS ----- Original Message ----- From: "Sally Norton" To: "Jerry Ricks" , "Histonet@lists.utsouthwestern.edu" Sent: Friday, April 3, 2015 1:42:09 PM Subject: RE: [Histonet] Kidney Stone Histology? The only thing we do with stones is send them to the Mayo clinic for chemical analysis. Sally Norton Seattle Children's -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Friday, April 03, 2015 11:38 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Stone Histology? How the heck do I process and section kidney stones? ?And what kind of stain do you like for them? Thanks Jerry Ricks Research Scientist University of Washington Department of Pathology ?? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From udsd007 <@t> gmail.com Sat Apr 4 22:54:22 2015 From: udsd007 <@t> gmail.com (Mike Andrews) Date: Sat Apr 4 22:54:28 2015 Subject: [Histonet] Kidney Stone Histology? In-Reply-To: <1357F84B33D39A46BA015A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.sea.kids> References: <1357F84B33D39A46BA015A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.sea.kids> Message-ID: What Everyone Else Wrote. They're stone (apatite; the kind I myself get) or hard crystals (uric acid). They're very unfriendly to a microtome blade. Getting a thin section of an apatite stone would require petrological thin-section techniques, which won't be in the repertoire of the typical soft-tissue histo lab. Think epoxy, abrasives, and time. Under a stereo scope, they can be amazing: covered with sharp corners and edges, and all the corners and edges have more corners and edges on them. For a big staghorn stone, a diamond rock saw might be required. Uric acid crystals aren't particularly better. But histo techniques pretty much don't apply. On Fri, Apr 3, 2015 at 1:42 PM, Norton, Sally < sally.norton@seattlechildrens.org> wrote: > The only thing we do with stones is send them to the Mayo clinic for > chemical analysis. > > Sally Norton > Seattle Children's > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks > Sent: Friday, April 03, 2015 11:38 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Kidney Stone Histology? > > How the heck do I process and section kidney stones? And what kind of > stain do you like for them? > > > Thanks > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information protected by law. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all copies > of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- 73 de Mike Andrews W5EGO From madary <@t> verizon.net Sun Apr 5 08:05:34 2015 From: madary <@t> verizon.net (madary@verizon.net) Date: Sun Apr 5 08:05:41 2015 Subject: [Histonet] kidney stone stain etc Message-ID: <28629564.780357.1428239134134.JavaMail.root@vznit170116.mailsrvcs.net> I a is too mu kossa or Dahls might &nbs Nick(Rocky) Madary, HT/HTL(ASCP)QIHC Jo On 04/04/15, histonet-request@lists.utsouthw Send Histonet mailing list submissions to [1]histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit [2]http://lists.utsouthwestern.edu/mailman/listinfo/ or, via email, send a message with subject or body 'help' [3]histonet-request@lists.utsouthwestern You can reach the person managing the list at < href="mailto:histonet-owner@lists.utsouthwestern. target="_blank">histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today 1. Kidney Stone Histology? (Jerry Ricks) 2 3. Re: Kidney Stone H ----------------------------------- ----------------------------------- Message: 1 Date: Fri, 3 From: Jerry Ricks <[4]rosenfeldtek@h Subject: [Histonet] Kidney Stone Histology? To Mes Content-Type: text/plain; charset="iso-8859-1" How the heck do I process and section kidney stones? And what kind of Thanks Jerry Ricks< University of Washington Department of P --------------------------- Message: 2 Date: Fri, 3 Apr 2015 18:42:09 +0000 Fr Subject: RE: [Histonet] Kidney Stone Histology? To: "'Jerry Ricks'" <[9]rosenfeldtek@hotmail.com&g "[10]Histonet@lists.utsouthwestern.edu&q <[11]histonet@lists.utsouthwestern.edu> Message-ID: <1357F84B33D39A46BA01[12]5A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.s ea.kids> Content-Type: text/plain; charset="us-ascii" The only thing we do with stones is send them to the Mayo clinic for chem Sally Norton Seattle Children's < -----Original Message----- From: lto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Friday, April 03, 2015 11:38 AM To: [14]Hist Subject: [Histonet] Kidney Stone His How the heck do I process and section kidney stones? An of stain do you like for them? Thanks Jerry Ricks Research Scientist University of Washington D _____ Histonet mailing list [16]http://lists.utsouthwestern.edu/mailman/listinfo/histon CONFIDENTIALITY NOTICE: This e-mail message, including any atta chments, is for the sole use of the intended recipient(s) and may contain c Any unauthorized r prohibited. If you are not the in the sender by reply e-mail and destroy all message. ------------------- Message: 3 Date: Fri, 3 Apr 2015 18:46:03 +0000 From: Pam Marcum <[17]mucram11@comcast.net> Su To: Sally Norton <[18]sally.norton@seattlechildrens.org> Cc: Histonet cks <[20]rosenfeldtek@hotmail.com> Message-ID:< /> <1966901990.6213413.142808676[21]3800.JavaMail.zimb ra@comcast.net> Content-Type: text/plain; charset=utf-8 Same here although we don't use Mayo.? They are gross only ship or store. ? Pam Marcum UAMS ----- Original Message ----- From: "Sally Norton" To: & ;[24]Histonet@lists.utsouthwestern.edu" <[25]histonet@lists.utsouthwestern.edu> Sent: Friday, A Subject: RE: [Histonet] Kidney Stone Histolog The only thing we do with stones is send them to the Mayo cl chemical analysis. Sally Norton Seattle Children -----Original Message----- From: [26]histonet-bounces@lists.utsouthwestern.edu [[27]mailto:histonet-bounces@lists.utsouthwestern.edu] On B Jerry Ricks Sent: Friday, April 03, 2015 11:38 AM To: Subject: [His How the heck do I process and se kind of stain do you like for the Thanks Jerry Ricks Research Scientis University of Washington Department of Pathology ? ? ? ? ? ? ?&nbs ? _____________________ Histonet mailing list [29]Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet attachments, is may contain confidential law. Any unauthorized review, use, prohibited. If you are not the intended recip the sender by reply e-mail and destroy all copies of t message. __________________________________________ Histonet mailing list [30]Histonet@lists.ut [31]http://lists.u --- ____________________________________ Histonet mailing list [32]Histonet@li [33]http://li End of Hist **************************************** References 1. 3D"mailto:histonet@lists.utsouthwest 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/hi 3. 3D"mailto:histonet-request@lists. 4. 3D"mailto:rosenfeldtek@hotmail.com" 5. 3D"mailto:Histonet@lists.utsouthweste 6. 3D"mailto:histonet@lists.utsouthwester 7. 3D"mailto:CC376D 8. 3D"mailto:s 9. file://localhost/tmp/3D"mailto 10. 3D"mailto:Histonet@lists.ut 11. 3D"mailto:histonet@lists.uts 12. 3D"mailto:5A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.sea.kids" 13. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 14. 3D"mailto:Histonet@lists.utsouthwestern.edu" 15. 3D"mailto:Histonet@lists.utsouthwestern.edu" 16. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 17. 3D"mailto:m 18. 3D"mailto:sally.norton@seattlechildrens.org" 19. 3D"mailto:histonet@lists.utsouthwestern.e 20. 3D"mailto:rosenfeldtek@hotmai 21. file://localhost/tmp/3D"ma 22. 3D"mailto:sally.norton@seattlechildrens.o 23. 3D"mailto:rosenfel 24. 3D"mailto:Histonet@lists.utsouthwestern.edu" 25. 3D"mailto:histonet@lists.utsouthwestern.edu" 26. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 27. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 28. 3D"mailto:Histonet@lists.utsouthwestern.edu" 29. 3D"mailto:Histonet@lists.utsouthwestern.edu" 30. file://localhost/tmp/3D"ma 31. 3D"http://lists.uts=/ 32. ="mailto:Histonet@lists.utsouthwestern.edu" 33. 3D"http://list=/ From rsrichmond <@t> gmail.com Sun Apr 5 14:17:46 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Apr 5 14:17:50 2015 Subject: [Histonet] Re: Kidney Stone Histology? Message-ID: I've been in pathology over 50 years, and I never heard of anybody trying to cut a section of a kidney stone. You sure this wasn't an April Fool grab? Stone analysis - once done chemically, now done mostly by physical methods - is of course clinically quite useful and is very often ordered. There aren't too many labs that do it - Louis Herring (see herringlab.com) is perhaps the oldest and best known. Bob Richmond Samurai Pathologist Maryville TN From wanpto <@t> aol.com Sun Apr 5 20:35:50 2015 From: wanpto <@t> aol.com (Wanda Jones) Date: Sun Apr 5 20:36:01 2015 Subject: [Histonet] Re: Histonet Digest, Vol 137, Issue 7 Message-ID: <42A0B921-16DF-4E32-AF5E-57B9DE6DCF98@aol.com> Wanda Platt Jones > On Apr 5, 2015, at 12:12 PM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Kidney Stone Histology? (Mike Andrews) > 2. kidney stone stain etc (madary@verizon.net) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 4 Apr 2015 22:54:22 -0500 > From: Mike Andrews > Subject: Re: [Histonet] Kidney Stone Histology? > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > What Everyone Else Wrote. > > They're stone (apatite; the kind I myself get) or hard crystals (uric > acid). They're very unfriendly to a microtome blade. Getting a thin section > of an apatite stone would require petrological thin-section techniques, > which won't be in the repertoire of the typical soft-tissue histo lab. > Think epoxy, abrasives, and time. Under a stereo scope, they can be > amazing: covered with sharp corners and edges, and all the corners and > edges have more corners and edges on them. For a big staghorn stone, a > diamond rock saw might be required. Uric acid crystals aren't particularly > better. But histo techniques pretty much don't apply. > > On Fri, Apr 3, 2015 at 1:42 PM, Norton, Sally < > sally.norton@seattlechildrens.org> wrote: > >> The only thing we do with stones is send them to the Mayo clinic for >> chemical analysis. >> >> Sally Norton >> Seattle Children's >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks >> Sent: Friday, April 03, 2015 11:38 AM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Kidney Stone Histology? >> >> How the heck do I process and section kidney stones? And what kind of >> stain do you like for them? >> >> >> Thanks >> >> Jerry Ricks >> Research Scientist >> University of Washington >> Department of Pathology >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is >> for the sole use of the intended recipient(s) and may contain confidential >> and privileged information protected by law. Any unauthorized review, use, >> disclosure or distribution is prohibited. If you are not the intended >> recipient, please contact the sender by reply e-mail and destroy all copies >> of the original message. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > 73 de > > Mike Andrews W5EGO > > > ------------------------------ > > Message: 2 > Date: Sun, 05 Apr 2015 08:05:34 -0500 (CDT) > From: madary@verizon.net > Subject: [Histonet] kidney stone stain etc > To: histonet@lists.utsouthwestern.edu > Message-ID: > <28629564.780357.1428239134134.JavaMail.root@vznit170116.mailsrvcs.net> > > Content-Type: text/plain; charset="UTF-8" > > > I a is too mu kossa or Dahls might > &nbs > Nick(Rocky) Madary, HT/HTL(ASCP)QIHC > Jo > On 04/04/15, histonet-request@lists.utsouthw > Send Histonet mailing list submissions to [1]histonet@lists.utsouthwestern.edu > To subscribe or unsubscribe via the World Wide Web, visit > [2]http://lists.utsouthwestern.edu/mailman/listinfo/ or, via email, send a message with subject or body 'help' [3]histonet-request@lists.utsouthwestern You can reach the person managing the list at > < href="mailto:histonet-owner@lists.utsouthwestern. target="_blank">histonet-owner@lists.utsouthwestern.edu > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today 1. Kidney Stone Histology? (Jerry Ricks) > 2 3. Re: Kidney Stone H ----------------------------------- ----------------------------------- > Message: 1 > Date: Fri, 3 From: Jerry Ricks <[4]rosenfeldtek@h Subject: [Histonet] Kidney Stone Histology? > To Mes Content-Type: text/plain; charset="iso-8859-1" > How the heck do I process and section kidney stones? And what kind of > Thanks > Jerry Ricks< University of Washington > Department of P > --------------------------- Message: 2 > Date: Fri, 3 Apr 2015 18:42:09 +0000 > Fr Subject: RE: [Histonet] Kidney Stone Histology? > To: "'Jerry Ricks'" <[9]rosenfeldtek@hotmail.com&g "[10]Histonet@lists.utsouthwestern.edu&q <[11]histonet@lists.utsouthwestern.edu> Message-ID: > <1357F84B33D39A46BA01[12]5A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.s > ea.kids> > Content-Type: text/plain; charset="us-ascii" > The only thing we do with stones is send them to the Mayo clinic for > chem Sally Norton > Seattle Children's > < -----Original Message----- > From: lto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry > Ricks Sent: Friday, April 03, 2015 11:38 AM > To: [14]Hist Subject: [Histonet] Kidney Stone His How the heck do I process and section kidney stones? An of stain do you like for them? > Thanks > Jerry Ricks > Research Scientist > University of Washington > D _____ Histonet mailing list > [16]http://lists.utsouthwestern.edu/mailman/listinfo/histon CONFIDENTIALITY NOTICE: This e-mail message, including any atta chments, is for the sole use of the intended recipient(s) and may > contain c Any unauthorized r prohibited. If you are not the in the sender by reply e-mail and destroy all message. > ------------------- Message: 3 > Date: Fri, 3 Apr 2015 18:46:03 +0000 From: Pam Marcum <[17]mucram11@comcast.net> > Su To: Sally Norton <[18]sally.norton@seattlechildrens.org> > Cc: Histonet cks > <[20]rosenfeldtek@hotmail.com> > Message-ID:< /> <1966901990.6213413.142808676[21]3800.JavaMail.zimb ra@comcast.net> > Content-Type: text/plain; charset=utf-8 > Same here although we don't use Mayo.? They are gross only ship or store. > ? > Pam Marcum > UAMS > ----- Original Message ----- > From: "Sally Norton" To: & ;[24]Histonet@lists.utsouthwestern.edu" > <[25]histonet@lists.utsouthwestern.edu> > Sent: Friday, A Subject: RE: [Histonet] Kidney Stone Histolog The only thing we do with stones is send them to the Mayo cl chemical analysis. > Sally Norton > Seattle Children -----Original Message----- > From: [26]histonet-bounces@lists.utsouthwestern.edu > [[27]mailto:histonet-bounces@lists.utsouthwestern.edu] On B Jerry Ricks > Sent: Friday, April 03, 2015 11:38 AM > To: Subject: [His How the heck do I process and se kind of stain do you like for the Thanks > Jerry Ricks > Research Scientis University of Washington > Department of Pathology > ? ? ? ? ? ? ?&nbs ? _____________________ Histonet mailing list > [29]Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > attachments, is may contain confidential law. Any unauthorized review, use, prohibited. If you are not the intended recip the sender by reply e-mail and destroy all copies of t message. > __________________________________________ Histonet mailing list > [30]Histonet@lists.ut [31]http://lists.u --- ____________________________________ Histonet mailing list > [32]Histonet@li [33]http://li End of Hist **************************************** > > References > > 1. 3D"mailto:histonet@lists.utsouthwest 2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/hi 3. 3D"mailto:histonet-request@lists. 4. 3D"mailto:rosenfeldtek@hotmail.com" > 5. 3D"mailto:Histonet@lists.utsouthweste 6. 3D"mailto:histonet@lists.utsouthwester 7. 3D"mailto:CC376D 8. 3D"mailto:s 9. file://localhost/tmp/3D"mailto 10. 3D"mailto:Histonet@lists.ut 11. 3D"mailto:histonet@lists.uts 12. 3D"mailto:5A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.sea.kids" > 13. ="mailto:histonet-bounces@lists.utsouthwestern.edu" > 14. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 15. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 16. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 17. 3D"mailto:m 18. 3D"mailto:sally.norton@seattlechildrens.org" > 19. 3D"mailto:histonet@lists.utsouthwestern.e 20. 3D"mailto:rosenfeldtek@hotmai 21. file://localhost/tmp/3D"ma 22. 3D"mailto:sally.norton@seattlechildrens.o 23. 3D"mailto:rosenfel 24. 3D"mailto:Histonet@lists.utsouthwestern.edu" 25. 3D"mailto:histonet@lists.utsouthwestern.edu" > 26. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" > 27. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" > 28. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 29. 3D"mailto:Histonet@lists.utsouthwestern.edu" > 30. file://localhost/tmp/3D"ma 31. 3D"http://lists.uts=/ > 32. ="mailto:Histonet@lists.utsouthwestern.edu" > 33. 3D"http://list=/ > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 137, Issue 7 > **************************************** From JMacDonald <@t> mtsac.edu Sun Apr 5 23:15:48 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Apr 5 23:16:12 2015 Subject: [Histonet] CSH Symposium Message-ID: Just a reminder that the annual CSH Symposium is just around the corner. We will be in Santa Cruz/Scotts Valley May 1-3. Please join us for some great workshops. CE documentation will be provided at the completion of each workshop for pre-registered attendees. If you'd like a PDF version please email me. http://californiahistology.org/events.html Thank you, Jennifer MacDonald From b-frederick <@t> northwestern.edu Mon Apr 6 08:19:53 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Apr 6 08:19:59 2015 Subject: [Histonet] Kidney Stone Histology? In-Reply-To: References: <1357F84B33D39A46BA015A8EC6ABCBD040EBFDB7@PPWEXD01a.childrens.sea.kids> Message-ID: <107bdb89d0314a7db98b84773bda8640@evcspmbx03.ads.northwestern.edu> Do you all see the picture of the stone on the NSH facebook page? It looks like an easter egg! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Andrews Sent: Saturday, April 04, 2015 10:54 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Kidney Stone Histology? What Everyone Else Wrote. They're stone (apatite; the kind I myself get) or hard crystals (uric acid). They're very unfriendly to a microtome blade. Getting a thin section of an apatite stone would require petrological thin-section techniques, which won't be in the repertoire of the typical soft-tissue histo lab. Think epoxy, abrasives, and time. Under a stereo scope, they can be amazing: covered with sharp corners and edges, and all the corners and edges have more corners and edges on them. For a big staghorn stone, a diamond rock saw might be required. Uric acid crystals aren't particularly better. But histo techniques pretty much don't apply. On Fri, Apr 3, 2015 at 1:42 PM, Norton, Sally < sally.norton@seattlechildrens.org> wrote: > The only thing we do with stones is send them to the Mayo clinic for > chemical analysis. > > Sally Norton > Seattle Children's > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks > Sent: Friday, April 03, 2015 11:38 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Kidney Stone Histology? > > How the heck do I process and section kidney stones? And what kind of > stain do you like for them? > > > Thanks > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential and privileged information protected by law. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- 73 de Mike Andrews W5EGO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anna.coffey <@t> nih.gov Mon Apr 6 10:26:34 2015 From: anna.coffey <@t> nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Mon Apr 6 10:38:26 2015 Subject: [Histonet] Anti-mouse TERT antibody Message-ID: <5C3E10119A1B824FBE92B08279F74A910178E2AD@msgb10.nih.gov> Does anyone know of a good anti-mouse TERT antibody? Appreciate your help! Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey@nih.gov 301-846-1730 From abadesuyi <@t> nrh-ok.com Mon Apr 6 13:08:16 2015 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Mon Apr 6 13:08:20 2015 Subject: [Histonet] Job Descriptions Message-ID: <04EE4F75BB5FB246ADB68D69B746044399095FC527@MAIL.nrhnt.nrh-ok.com> Hi everyone, How are you guys doing in histoland? I hope you are all doing well. I am in the process of reviewing the job descriptions of my staff and would really appreciate your input. I want to review the job descriptions of the positions stated below; 1. Histology Supervisor HT(ASCP)HTL, QIHC, QLS 2. Histotechnologist i.e. HTL(ASCP) Certified 3. Histotechnician i.e. HT(ASCP) Certified 4. Non - Certified Histotechnician. Best regards, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor & Clinical Coordinator Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From mucram11 <@t> comcast.net Mon Apr 6 13:42:10 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon Apr 6 13:42:27 2015 Subject: [Histonet] Posting for colleague Please help Message-ID: <1011252859.6772502.1428345730905.JavaMail.zimbra@comcast.net> I am currently trying to stain L6 vertebrae from rabbits. They have been decalcified and paraffin processed properly. I've tried cutting at both 5 and 10 microns and my tissue is still not sticking to my slides. I know my sectioning is fine because I'm successful with every other tissue I've ever sectioned and stained. For some reason the bone I'm using won't stick to any slides. I was using charged slides and I even tried poly-L-lysine slides, but the bone keeps coming up even before I attempted to stain them. I've even tried leaving them in the incubator for more than the usual 48-72 hours. I know it's possible to do other stains beside H&E on bone, but I think my main issue is just getting good contact between the tissue and slide. If you have any advice or thoughts, I would love to hear them. ? I will get the messages to him ASAP. ? Pam From Cathie.Crukley <@t> lhsc.on.ca Tue Apr 7 10:56:38 2015 From: Cathie.Crukley <@t> lhsc.on.ca (Cathie Crukley) Date: Tue Apr 7 10:56:49 2015 Subject: [Histonet] Levai-Laczko stain Message-ID: <5523C5F6020000BF00036DB5@lhscgwiao.lhsc.on.ca> Hi all, Does anyone have a reference or method for the Levai-Laczko stain? Thanks, Cathie Crukley London, ON -------------------------------------------------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. From BZIMMERM <@t> gru.edu Tue Apr 7 11:22:26 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Apr 7 11:22:32 2015 Subject: [Histonet] HISTOPALOOZA GEORGIA SOCIETY FOR HISTOTECHNOLOGY APRIL 17 - APRIL 19 Message-ID: We are beyond procrastination, but you can still join us at HISTOPALOOZA! for an AFFORDABLE adventure. Early registration will insure your waived entrance gate fees and your registration packet. Lollygaggers are welcome for the affordable rate of $135 "all inclusive" for up to 15 CEUs (up to 15 for all the overachievers). Early registration insures adequate handouts for each workshop. Don't forget to bring your swimsuit for the heated pool. If you still need a room call the Legacy Lodge at Lake Lanier for available room rates. If no rooms are available give me a call and see what other options are available. ( I'm not talking about Spring Break- "Girls gone wild" piling up in rooms either.) hehe We can check out hotels in the surrounding area. If you can't attend HISTOPALOOZA, North Carolina has their symposium this upcoming weekend in Durham Raleigh-home of the Duke Blue Devils. Looking forward to some education, fun, relaxation, and networking in a couple of weeks. Great weekend at a great price!! Wanda K. Simons HT (ASCP) GSH President /BZ From dermacklab <@t> gmail.com Tue Apr 7 12:59:01 2015 From: dermacklab <@t> gmail.com (Jeff McKenna) Date: Tue Apr 7 12:59:10 2015 Subject: [Histonet] Re: Anyone need a Benchmark Ultra? In-Reply-To: References: Message-ID: Is anyone out there looking for an extra Ventana Benchmark Ultra for IHC/ISH? I have one in good shape that is not being used anymore and I am looking to free up some space. Email me if you are interested and I'll give you more info on it. Jeff Mack dermacklab@gmail.com On Tue, Mar 17, 2015 at 1:55 PM, Jeff McKenna wrote: > Is anyone looking for an extra Ventana Benchmark Ultra for IHC/ISH? I have > one in good shape that is not being used anymore and I am looking to free > up some space. Email me if you are interested and I'll give you more info > on it. > > > Jeff Mack > > dermacklab@gmail.com > > (302) 827-3685 > From gayle.callis <@t> bresnan.net Tue Apr 7 16:24:17 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Apr 7 16:24:28 2015 Subject: [Histonet] Re: Laczko/Levai stain protocol Message-ID: <000101d07179$34a82280$9df86780$@bresnan.net> I found several pages from Hayat's book Stains and Cytochemical Staining 1993 (for EM) with original authors cited after a Google search, key words Laczko and Levai staining protocol. Go to this long link and read on hints to improve staining plus the protocol. https://books.google.com/books?id=oGj7MLioFlQC&pg=PA60&lpg=PA60&dq=Levai-Lac zko+procedure&source=bl&ots=5i96tYhIh1&sig=iXOwXifgBUsaWGjdOGya_zjOPlA&hl=en &sa=X&ei=IjYkVcKIN5GrogStzYLQAw&ved=0CFIQ6AEwCTgK#v=onepage&q=Levai-Laczko%2 0procedure&f=false The Google search turned up many publications using this stain for undecalcified bone in PMMA, but you would have to access the journals for the publications, even the original publication for the stain by Laczko and Levai. Cartilage and Bone (Laczko and Levai, 1975) Azure II Methylene blue Safranin O Tissue was fixed with mixture of gluteraldehyde and formaldehyde 2 -4 hr @ RT. I assume these are tiny (minced) pieces of tissue for EM. Bone was decalcified for 2 weeks at 4C, postfixed with osmium tetroxide 2 -3 hr RT, embedded in Durcupan, an EM resin. I doubt people embedding mineralized bone in MMA/PMMA will want to fix in gluteraldehyde, but rather NBF and not post fix in osmium tetroxide. The stain, as seen in many cited references was used for thin sections of bone. You can pick up those references via Google for more on methods and materials for undecalcified bone in PMMA/MMA. Azure II/Methylene Blue Working Solution Azure II 1% 10 ml Methylene Blue 1% 10 ml Sodium carbonate 1% 20 ml Protocol 1. Stain in working solution for 2 -5 min at 50 - 60C 2. Rinse in 0.5% sodium carbonate followed by distilled water 3. Counterstain with 0.5% safranin aq. 3 min at 35 - 40C and rinse with distilled water, dehydrate. Results Chromatin, nucleoli violet blue Cytoplasm blue Erythrocytes dark blue cartilage matrix light blue bone matrix bright red The search found many photos of bone with this stain to give you examples. Check out these links: https://www.unizahnklinik-wien.at/en/science/tangl_laboratory/resources.php and geosoft.ru/stati/de%20Sanctis%202010_J%20Clin%20Perio_.pdf by M de Sanctis - ?2010 Material and Methods: Eight beagle dogs received implants randomly installed into ..... Levai. Laczko staining. Original magnification x 2.5. Fig. 9. Most apical . Good luck Gayle Callis HTL/HT/MT(ASCP) From amylee053 <@t> gmail.com Tue Apr 7 16:30:56 2015 From: amylee053 <@t> gmail.com (Amy Lee) Date: Tue Apr 7 16:30:59 2015 Subject: [Histonet] test Message-ID: From amylee053 <@t> gmail.com Tue Apr 7 16:32:58 2015 From: amylee053 <@t> gmail.com (Amy Lee) Date: Tue Apr 7 16:33:02 2015 Subject: [Histonet] type IV collagen antibody Message-ID: Hello histonet, Could anyone recommend a type IV collagen antibody work on FFPE mouse tissue? Thanks in advance, Amy From gayle.callis <@t> bresnan.net Tue Apr 7 16:56:33 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Apr 7 16:56:42 2015 Subject: [Histonet] Re: Pam Marcum colleague losing bone sections from slides Message-ID: <000001d0717d$b658ada0$230a08e0$@bresnan.net> >From Pam: I am currently trying to stain L6 vertebrae from rabbits. They have been decalcified and paraffin processed properly. I've tried cutting at both 5 and 10 microns and my tissue is still not sticking to my slides. I know my sectioning is fine because I'm successful with every other tissue I've ever sectioned and stained. For some reason the bone I'm using won't stick to any slides. I was using charged slides and I even tried poly-L-lysine slides, but the bone keeps coming up even before I attempted to stain them. I've even tried leaving them in the incubator for more than the usual 48-72 hours. I know it's possible to do other stains beside H&E on bone, but I think my main issue is just getting good contact between the tissue and slide. If you have any advice or thoughts, I would love to hear them. I will get the messages to him ASAP. Pam ************************************************************************* What was meant by incubator and at what temperature? It helps to dry sections FLAT, at 37 to 40C for several days. Do NOT dry at 60C. If the sections are not staying on plus charge or poly L lysine coated slides, then use chrome gelatin subbing solution in a water bath OR by pre-subbing clean microscope slides. This is the Chrome gelatin protocol that worked for our huge decalcified bone sections and or problem bone sections. Chrome Gelatin Subbing Solution: Section/Slide Coating Adhesive 0.1 g Chromium Potassium Sulfate (this is toxic. Collect for proper disposal, not down the drain is you pre-sub the slides). 1.0 g Gelatin: 100 bloom, Sigma. For large bone sections, use 200 or 300 bloom gelatin, Sigma). 200 and 300 bloom gelatins are very large gelatin molecules made from pig collagen. 100 bloom is a much smaller molecule than 200 bloom. Do NOT use household (cooking) gelatin used for cooking. Buy the pure gelatins only. 1 liter Distilled Water Dissolve chromium potassium sulfate and gelatin in hot but not boiling water. Cool subbing solution before use, and store in refrigerator. If gelatin gets growth, discard, make new. A few crystals of Thymol in stock subbing solution can help prevent growth. DO NOT USE PLUS CHARGE SLIDES WITH SUBBING SOLUTION. GELATIN COATS OVER A PLUS CHARGE COATING AND NEGATES THE POSITIVE CHARGE. For presubbing glass slides, wash these by dipping in acetone, air dry before using the pre-subbing protocol to get rid of any greasy/oily residues on glass surface. If you put the subbing solution in a water bath, uncoated, glass slides will work fine without further washing. You can do either of the following: 1. Add 10 ml subbing solution to a warm water bath for paraffin sections. Then mount sections onto the cleaned glass slide, drain, and air dry, store in a cool, dry place. 2. Dip acetone washed, dry slides into subbing solution, air dry, and store in a dust free area. Box subbed slides and store until needed. If you get background staining with hematoxylin (hematoxylin stains gelatin) then dip pre-subbed slides in NBF ~10 times, rinse with distilled water, air dry and store slides. The aldehyde fixative cross links the gelatin to some degree, but still allows section to adhere without annoying background staining. Pick up sections from water bath drain and lay flat to dry at 40C for several days. You will not need extra subbing solution in the water bath if using presubbed slides. IF all else fails, try Sterchi tape transfer method with packaging tape. I have the method with photos and publication, and will send privately. Good luck Gayle Callis HTL/HT/MT(ASCP) From liz <@t> premierlab.com Tue Apr 7 17:06:17 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 7 17:06:15 2015 Subject: [Histonet] type IV collagen antibody In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79F1808D@SBS2K8.premierlab.local> Amy It?s been some time but we have had success in the past with the following antibodies ? the SouthernBio and Abcam antibodies listed below Antibody Vendor Catalog Number Clone Host Hu Rat Rab Ms Pm Sh Gt Pig GP Dog Comments Collagen IV SouternBio 0340-01 UNLB Goat X X X Collagen IV abcam ab6586 Poly Rabbit X X Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, April 07, 2015 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] type IV collagen antibody Hello histonet, Could anyone recommend a type IV collagen antibody work on FFPE mouse tissue? Thanks in advance, Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Tue Apr 7 17:22:39 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Apr 7 17:22:46 2015 Subject: [Histonet] Re: Pam Marcum colleague losing bone sections from slides In-Reply-To: <000001d0717d$b658ada0$230a08e0$@bresnan.net> References: <000001d0717d$b658ada0$230a08e0$@bresnan.net> Message-ID: Have you ever tried blotting your slides dry before putting them into the oven? The veteran who taught me this trick used it on brain tissues from the Coroner's office (in the early 80s--don't wanna offend anyone) which were grossed very thickly and were always poorly processed. She never used charged slides or additives in her waterbath. She claimed she was the only one in her lab who was "allowed" to cut this stuff because everyone else's slides had tissue loss! I can tell you from my experience that it works well for toenail which is notorious for detaching from slides. I've used it on many other tissues as well. Anyway, press a slightly moistened clean L'Absorb or paper towel down onto the section after microtomy. You don't want the paper towel soaking wet--just damp. This will effectively wick the section of any excess moisture. Then incubate and stain as usual. Good luck. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, April 07, 2015 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Pam Marcum colleague losing bone sections from slides >From Pam: I am currently trying to stain L6 vertebrae from rabbits. >They have been decalcified and paraffin processed properly. I've tried cutting at both 5 and 10 microns and my tissue is still not sticking to my slides. I know my sectioning is fine because I'm successful with every other tissue I've ever sectioned and stained. For some reason the bone I'm using won't stick to any slides. I was using charged slides and I even tried poly-L-lysine slides, but the bone keeps coming up even before I attempted to stain them. I've even tried leaving them in the incubator for more than the usual 48-72 hours. I know it's possible to do other stains beside H&E on bone, but I think my main issue is just getting good contact between the tissue and slide. If you have any advice or thoughts, I would love to hear them. I will get the messages to him ASAP. Pam ************************************************************************* What was meant by incubator and at what temperature? It helps to dry sections FLAT, at 37 to 40C for several days. Do NOT dry at 60C. If the sections are not staying on plus charge or poly L lysine coated slides, then use chrome gelatin subbing solution in a water bath OR by pre-subbing clean microscope slides. This is the Chrome gelatin protocol that worked for our huge decalcified bone sections and or problem bone sections. Chrome Gelatin Subbing Solution: Section/Slide Coating Adhesive 0.1 g Chromium Potassium Sulfate (this is toxic. Collect for proper disposal, not down the drain is you pre-sub the slides). 1.0 g Gelatin: 100 bloom, Sigma. For large bone sections, use 200 or 300 bloom gelatin, Sigma). 200 and 300 bloom gelatins are very large gelatin molecules made from pig collagen. 100 bloom is a much smaller molecule than 200 bloom. Do NOT use household (cooking) gelatin used for cooking. Buy the pure gelatins only. 1 liter Distilled Water Dissolve chromium potassium sulfate and gelatin in hot but not boiling water. Cool subbing solution before use, and store in refrigerator. If gelatin gets growth, discard, make new. A few crystals of Thymol in stock subbing solution can help prevent growth. DO NOT USE PLUS CHARGE SLIDES WITH SUBBING SOLUTION. GELATIN COATS OVER A PLUS CHARGE COATING AND NEGATES THE POSITIVE CHARGE. For presubbing glass slides, wash these by dipping in acetone, air dry before using the pre-subbing protocol to get rid of any greasy/oily residues on glass surface. If you put the subbing solution in a water bath, uncoated, glass slides will work fine without further washing. You can do either of the following: 1. Add 10 ml subbing solution to a warm water bath for paraffin sections. Then mount sections onto the cleaned glass slide, drain, and air dry, store in a cool, dry place. 2. Dip acetone washed, dry slides into subbing solution, air dry, and store in a dust free area. Box subbed slides and store until needed. If you get background staining with hematoxylin (hematoxylin stains gelatin) then dip pre-subbed slides in NBF ~10 times, rinse with distilled water, air dry and store slides. The aldehyde fixative cross links the gelatin to some degree, but still allows section to adhere without annoying background staining. Pick up sections from water bath drain and lay flat to dry at 40C for several days. You will not need extra subbing solution in the water bath if using presubbed slides. IF all else fails, try Sterchi tape transfer method with packaging tape. I have the method with photos and publication, and will send privately. Good luck Gayle Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From cfields <@t> mlkch.org Tue Apr 7 17:37:48 2015 From: cfields <@t> mlkch.org (Carol Fields) Date: Tue Apr 7 17:38:05 2015 Subject: FW: [Histonet] Re: Pam Marcum colleague losing bone sections from slides References: <000001d0717d$b658ada0$230a08e0$@bresnan.net> Message-ID: -----Original Message----- From: Carol Fields Sent: Tuesday, April 07, 2015 3:37 PM To: 'Cooper, Brian' Cc: 'histonet-bounces@lists.utsouthwestern.edu' Subject: RE: [Histonet] Re: Pam Marcum colleague losing bone sections from slides I've used that also many times. It really does work. Carole Fields, HT (ASCP) Lead Histotechnologist, Pathology Laboratory Martin Luther King Jr. Community Hospital 1680 E. 120th Street Los Angeles, CA 90059 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Tuesday, April 07, 2015 3:23 PM To: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Pam Marcum colleague losing bone sections from slides Have you ever tried blotting your slides dry before putting them into the oven? The veteran who taught me this trick used it on brain tissues from the Coroner's office (in the early 80s--don't wanna offend anyone) which were grossed very thickly and were always poorly processed. She never used charged slides or additives in her waterbath. She claimed she was the only one in her lab who was "allowed" to cut this stuff because everyone else's slides had tissue loss! I can tell you from my experience that it works well for toenail which is notorious for detaching from slides. I've used it on many other tissues as well. Anyway, press a slightly moistened clean L'Absorb or paper towel down onto the section after microtomy. You don't want the paper towel soaking wet--just damp. This will effectively wick the section of any excess moisture. Then incubate and stain as usual. Good luck. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, April 07, 2015 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Pam Marcum colleague losing bone sections from slides >From Pam: I am currently trying to stain L6 vertebrae from rabbits. >They have been decalcified and paraffin processed properly. I've tried cutting at both 5 and 10 microns and my tissue is still not sticking to my slides. I know my sectioning is fine because I'm successful with every other tissue I've ever sectioned and stained. For some reason the bone I'm using won't stick to any slides. I was using charged slides and I even tried poly-L-lysine slides, but the bone keeps coming up even before I attempted to stain them. I've even tried leaving them in the incubator for more than the usual 48-72 hours. I know it's possible to do other stains beside H&E on bone, but I think my main issue is just getting good contact between the tissue and slide. If you have any advice or thoughts, I would love to hear them. I will get the messages to him ASAP. Pam ************************************************************************* What was meant by incubator and at what temperature? It helps to dry sections FLAT, at 37 to 40C for several days. Do NOT dry at 60C. If the sections are not staying on plus charge or poly L lysine coated slides, then use chrome gelatin subbing solution in a water bath OR by pre-subbing clean microscope slides. This is the Chrome gelatin protocol that worked for our huge decalcified bone sections and or problem bone sections. Chrome Gelatin Subbing Solution: Section/Slide Coating Adhesive 0.1 g Chromium Potassium Sulfate (this is toxic. Collect for proper disposal, not down the drain is you pre-sub the slides). 1.0 g Gelatin: 100 bloom, Sigma. For large bone sections, use 200 or 300 bloom gelatin, Sigma). 200 and 300 bloom gelatins are very large gelatin molecules made from pig collagen. 100 bloom is a much smaller molecule than 200 bloom. Do NOT use household (cooking) gelatin used for cooking. Buy the pure gelatins only. 1 liter Distilled Water Dissolve chromium potassium sulfate and gelatin in hot but not boiling water. Cool subbing solution before use, and store in refrigerator. If gelatin gets growth, discard, make new. A few crystals of Thymol in stock subbing solution can help prevent growth. DO NOT USE PLUS CHARGE SLIDES WITH SUBBING SOLUTION. GELATIN COATS OVER A PLUS CHARGE COATING AND NEGATES THE POSITIVE CHARGE. For presubbing glass slides, wash these by dipping in acetone, air dry before using the pre-subbing protocol to get rid of any greasy/oily residues on glass surface. If you put the subbing solution in a water bath, uncoated, glass slides will work fine without further washing. You can do either of the following: 1. Add 10 ml subbing solution to a warm water bath for paraffin sections. Then mount sections onto the cleaned glass slide, drain, and air dry, store in a cool, dry place. 2. Dip acetone washed, dry slides into subbing solution, air dry, and store in a dust free area. Box subbed slides and store until needed. If you get background staining with hematoxylin (hematoxylin stains gelatin) then dip pre-subbed slides in NBF ~10 times, rinse with distilled water, air dry and store slides. The aldehyde fixative cross links the gelatin to some degree, but still allows section to adhere without annoying background staining. Pick up sections from water bath drain and lay flat to dry at 40C for several days. You will not need extra subbing solution in the water bath if using presubbed slides. IF all else fails, try Sterchi tape transfer method with packaging tape. I have the method with photos and publication, and will send privately. Good luck Gayle Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fernandom.munoz <@t> usc.es Wed Apr 8 05:12:14 2015 From: fernandom.munoz <@t> usc.es (fernandom.munoz@usc.es) Date: Wed Apr 8 05:12:22 2015 Subject: [Histonet] Re: Levai-Laczko stain In-Reply-To: References: Message-ID: <20150408121214.15872nkip58n96as@correoweb.usc.es> 1. 30% H2O2 5 min. under movement. 2. wash in water 2x 3. 5% Acetic acid 1 min. 4. wash in water 2x 5. solution 1 20 min. 6. wash in water 2x 7. solution 2, piece by piece 8. wash in water and dry. Solution 1: 1 part Azur II (1%) + 1 part Methylen blue (1%) + 2 parts Na2CO3 (1%). Solution 2: Pararosanillin (1%). All solutions in distilled water. NEVER alcohol or acetone in plastic sections. Fernando Mar?a Mu?oz Guz?n Facultad de Veterinaria Campus Universitario s/n 27002 Lugo (Espa?a) Tfno: (+34)600940225 From jennifer.harvey <@t> Vanderbilt.Edu Wed Apr 8 08:25:08 2015 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Wed Apr 8 08:26:43 2015 Subject: [Histonet] Sihler stain for whole-mount nerves Message-ID: A fellow lab is asking if I know anyone doing a Sihler stain to visualize whole-mount nerves. The protocol calls for chloral hydrate, which they cannot get as a DEA class IV substance. Better yet, are there alternatives to 1% w/v chloral hydrate/12% glycerol aqueous solutions for whole mount stainings? Looks like a very interesting stain but I can say I never heard of it. Any help would be appreciated. Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 From cindy38017 <@t> yahoo.com Wed Apr 8 08:35:22 2015 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Wed Apr 8 08:35:29 2015 Subject: [Histonet] paraform cassettes Message-ID: <1428500122.63727.YahooMailBasic@web163901.mail.gq1.yahoo.com> From cindy38017 <@t> yahoo.com Wed Apr 8 08:36:00 2015 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Wed Apr 8 08:36:08 2015 Subject: [Histonet] paraform cassettes Message-ID: <1428500160.37094.YahooMailBasic@web163905.mail.gq1.yahoo.com> From cindy38017 <@t> yahoo.com Wed Apr 8 08:38:53 2015 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Wed Apr 8 08:39:00 2015 Subject: [Histonet] paraform cassettes Message-ID: <1428500333.20199.YahooMailBasic@web163904.mail.gq1.yahoo.com> We purchased several sizes of the paraform cassettes and decided that they did not work the way we had hoped. If anyone is interested, I would be happy to send them to you instead of throwing them away. Thanks, Cindy From Sheri.Meilus <@t> va.gov Wed Apr 8 09:20:53 2015 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Wed Apr 8 09:21:36 2015 Subject: [Histonet] Vacancy Announcements C.W. Bill Young VAHCS Message-ID: Hello Histonetters, Due to retirements, the following vacancy (times 2) has been posted on USAJOBS: Histotechnologist (GS-0601-09): The open period for this announcement is from April 6, 2015 through April 21, 2015. Vacancy Identification Number (VIN) 1373443. If you are interested or know of someone who would like to apply, please do so through USAJOBS. I have inserted the hyperlink for the position below. https://www.usajobs.gov/Search?Keyword=Histotechnologist&Location=Bay+Pines%2C+Florida&search=Search&AutoCompleteSelected=true Bay Pines is located in the greater Tampa Bay/St. Petersburg area of Florida and the medical center sits on the shore of Boca Ciega Bay. It is a beautiful campus with lakes and walking trails. We have a state of the art Histology laboratory and a professional and friendly atmosphere. If you are looking for a great place to work with fantastic benefits, you've found it! S Sheri Meilus HT (ASCP) QIHC Anatomic Pathology Supervisor Bay Pines VAHCS Sheri.meilus@va.gov 727-398-6661 ext. 4596 From relia1 <@t> earthlink.net Wed Apr 8 10:08:32 2015 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Apr 8 10:08:39 2015 Subject: [Histonet] RELIA HOT Job Alert 4-8-2015 Hope your having a Great Day!!! Message-ID: <001901d0720d$e10c10b0$a3243210$@earthlink.net> Hi Histonetters!!! I hope you had a wonderful Holiday and Spring weekend with your family and friends. Look we are halfway to the next beautiful Spring Weekend!! I wanted to send a quick note to tell you about the positions that I am working on and am most excited about. Why am I excited about these positions? Because these clients offer excellent compensation, benefits, relocation assistance and most importantly they are ready to interview and hire ASAP. All of these positions are full time and permanent These are the histology positions that i am most excited about! LEADERSHIP/SUPERVISORY: AP Manager - Chicago Area Histology Supervisor - Flagstaff, AZ IHC Specialist - Shenandoah Valley, VA Lead Histotechnologist - St. Louis, MO Field Applications Specialist - Open territory base from anywhere TECHNICIANS/TECHNOLOGISTS: Dermpath Histotech - Birmingham, AL exciting new client! Histotechnologist - Charlotte, NC great benefits and stability! Histotechnician - Hammond, IN (near Chicago) ASCP or elig.! IHC Specialist - Shenandoah Valley, VA Newly created position! Dermpath Histo - Kansas City, MO CLIA Qual/Gross-learn mohs Histotechnician - Nashville, TN nice benefits, evening shift Lead Histotech - St. Louis, MO beautiful lab, and great team! Field Applications Specialist - Open territory base from anywhere If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer, You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 or relia1@earthlink.net or you can always catch me on cell at 407-353-5070. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting From j.benavides <@t> eae.csic.es Wed Apr 8 10:34:47 2015 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Wed Apr 8 10:40:47 2015 Subject: [Histonet] Buffered formalin In-Reply-To: <1428500333.20199.YahooMailBasic@web163904.mail.gq1.yahoo.com> References: <1428500333.20199.YahooMailBasic@web163904.mail.gq1.yahoo.com> Message-ID: <55254A97.7090405@eae.csic.es> Hello, I was wondering in buffered formalin (for tissue samples fixation) could be prepared like follows: 100 ml PBS 10x 100 ml Formaldehyde 40% 800 ml Distilled water Thanks in advanced for your thoughts!! Regards Julio From PAMarcum <@t> uams.edu Wed Apr 8 11:02:22 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Apr 8 11:02:40 2015 Subject: [Histonet] HT or HTL Position in Little Rock AR Message-ID: We have an immediate opening for full time HT or HTL for early morning shift at the University of Arkansas for Medical Sciences in Little Arkansas. We have great benefits, 2 weeks' vacation and sick leave. Since it is a state facility we also have all Federal holidays and you birthday off. The person will be required to have ASCP registry and computer knowledge. We are a very progressive Histology Laboratory in the heart of Arkansas. If you have any questions please e-mail me. I am sorry we do not pay relocation or bonuses. Please do not contact me if you are a recruiter as we are not allowed to use your service and I do not wish to waste your time or mine. Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From j.benavides <@t> eae.csic.es Wed Apr 8 11:07:17 2015 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Wed Apr 8 11:13:00 2015 Subject: [Histonet] Buffered formalin In-Reply-To: References: <1428500333.20199.YahooMailBasic@web163904.mail.gq1.yahoo.com> <55254A97.7090405@eae.csic.es> Message-ID: <55255235.1030401@eae.csic.es> Dear Bernice, thanks a lot for your reply. The thing is that we received some samples fixed in that solution (buffered formalin made with PBS), and I was wondering if this could interfere with immunohistochemestry or normal HE staining. BTW, writing from Spain, I guess commercial buffered formalin is cheap as well, but we have always made it in house. cheers julio On 08/04/2015 18:08, Bernice Frederick wrote: > Normally in my opinion, no. Usually the buffer is sodium phosphate monobasic and sodium phosphate dibasic. You can google the formula or as we get ours from Fisher it will tell you the mix. There is also millinog's formula and it is buffered but I don't use it so can't think of it off the top of my head. A 5 gallon cube from Fisher is like 25$ here (I don't know where you are) so it's cheap to buy... > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julio Benavides > Sent: Wednesday, April 08, 2015 10:35 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Buffered formalin > > Hello, > > I was wondering in buffered formalin (for tissue samples fixation) could be prepared like follows: > > 100 ml PBS 10x > 100 ml Formaldehyde 40% > 800 ml Distilled water > > Thanks in advanced for your thoughts!! > > Regards > > Julio > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy <@t> SpringfieldClinic.com Wed Apr 8 11:35:49 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Wed Apr 8 11:36:05 2015 Subject: [Histonet] Formaldehyde filters on Grossing station Message-ID: <9B1A1501A800064397369BD8072E6BCAB5239D@E2K10DB.springfieldclinic.com> Does anybody remember how to test whether a formaldehyde filter for a grossing station is exhausted? If I remember right there is some sort of test where you remove a couple of granules and cut the granules in half. The color of the cut service is used somehow. Also I know that changing formaldehyde filters on ductless workstations needs to be done regularly. Can anyone share how often they change the formaldehyde filters for a gross lab senior or a small station such as a HYperclean? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Timothy.Morken <@t> ucsf.edu Wed Apr 8 12:06:27 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Apr 8 12:23:07 2015 Subject: [Histonet] Cilia embedding for EM Message-ID: <761E2B5697F795489C8710BCC72141FF36801FF6@ex07.net.ucsf.edu> Anyone doing cilia brush bx embedding for EM - do you have a proven method for using agar/gel for the pellet to avoid manual processing? We tried Histogel but it falls apart too easily so will move to trying agar. But if anyone has a proven method it would be helpful to try it. Thanks! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org From hymclab.hymclab <@t> ministryhealth.org Wed Apr 8 14:52:07 2015 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Wed Apr 8 14:52:21 2015 Subject: [Histonet] RE: Tri-State Histology Symposium In-Reply-To: References: <43963a03b96143e0ad30730d9d52461c@PHUSCB-SP37MB04.genoptix.org> Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Monday, March 02, 2015 11:07 AM To: 'Teri Johnson'; Colleen_Herring@bshsi.org Cc: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tri-State Histology Symposium Dear Histonetters: You are invited to join the histology societies of Wisconsin, Iowa and Minnesota as we celebrate "Hats Off to Histology" at the 2015 Tri-State Histology Symposium, May 6-8 at The Madison Concourse Hotel and Governors Club in Madison, Wisconsin. For program, registration and vendor/exhibit information contact the following representatives: Wisconsin: Kathryn Stoll kstoll@mcw.edu Iowa: Judi Stasko judith.stasko@ars.usda.gov Minnesota: Lois Rowe rowe.lois@mayo.edu Vendor/Exhibit: Dawn Schneider dawn.schneider@ministryhealth.org ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From fpearsa <@t> clemson.edu Wed Apr 8 15:52:35 2015 From: fpearsa <@t> clemson.edu (Fran Pearsall) Date: Wed Apr 8 15:52:37 2015 Subject: [Histonet] Bile Stain Message-ID: <55259513.9060808@clemson.edu> I would like to ask if it's possible to do a Fouchet's reaction in the first part of the stain; then instead of doing the Van Giesens as the counter stain can I just use Nuclear Fast Red? For some reason my Steins Iodine stain isn't getting the bile green? Thank you for any suggestions. From JMacDonald <@t> mtsac.edu Wed Apr 8 16:02:18 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Apr 8 16:02:37 2015 Subject: [Histonet] Bile Stain In-Reply-To: <55259513.9060808@clemson.edu> Message-ID: Yes this possible. Sent from my iPhone > On Apr 8, 2015, at 1:54 PM, Fran Pearsall wrote: > > I would like to ask if it's possible to do a Fouchet's reaction in the > first part of the stain; then instead of doing the Van Giesens as the > counter stain can I just use Nuclear Fast Red? For some reason my Steins > Iodine stain isn't getting the bile green? Thank you for any suggestions. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Apr 8 19:15:00 2015 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Apr 8 19:15:04 2015 Subject: [Histonet] type IV collagen antibody Message-ID: Hi, The one from AbCam Cat# ab6586 works nicely. I have also used Dako's Coll IV with some success. Amos On Wed, Apr 8, 2015 at 1:00 PM, wrote: > Message: 4 > Date: Tue, 7 Apr 2015 14:32:58 -0700 > From: Amy Lee > Subject: [Histonet] type IV collagen antibody > To: histonet@lists.utsouthwestern.edu > Message-ID: > vYJ3MpG5G4jMoGiA09KYOvj4fee_3KZr-bYjee8tg@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Hello histonet, > > Could anyone recommend a type IV collagen antibody work on FFPE mouse > tissue? > > Thanks in advance, > > Amy > From sccrshlly <@t> yahoo.com Wed Apr 8 20:13:12 2015 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Wed Apr 8 20:13:19 2015 Subject: [Histonet] RE: Formaldehyde filters In-Reply-To: <1708285038.2967870.1428541897547.JavaMail.yahoo@mail.yahoo.com> References: <1708285038.2967870.1428541897547.JavaMail.yahoo@mail.yahoo.com> Message-ID: <134663828.2944066.1428541992802.JavaMail.yahoo@mail.yahoo.com> I am not sure about the color change you are referencing, unless it is the change that occurs with potassium permanganate filters, which turn black when exhausted. We have a countertop gross station with two Potassium Permanganate filters that we change every 6 months.? Keep in mind we do very small specimens and deal with very small amounts of formalin.? If you use the Potassium permanganate filters (which are no more expensive than charcoal through Mopec), then you should be able to come up with a changing schedule that will work with your workflow. Try not to get the potassium permanganate anywhere on the floor or counter or your clothes though....the dust is a tan color, but reacts with moisture and turns purple! >Does anybody remember how to test whether a formaldehyde filter for a grossing station is exhausted?? If I remember right there is some sort of test where you remove a couple of granules and cut the granules in half.? The color of the cut service is used somehow. Also I know that changing formaldehyde filters on ductless workstations needs to be done regularly.? Can anyone share how often they change the formaldehyde filters for a gross lab senior or a small station such as a HYperclean? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy@SpringfieldClinic.com From TMcNemar <@t> lmhealth.org Thu Apr 9 06:10:10 2015 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Apr 9 06:10:23 2015 Subject: [Histonet] Histology position in Newark, Ohio (approximately 30 miles east of Columbus) Message-ID: Hello all, Licking Memorial Hospital has an opening for a full time certified histology tech. Dayshift position, 7:30am to 4:00pm, Monday through Friday with no weekends or holidays. If interested check us out and apply online at www.lmhealth.org. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From TMcNemar <@t> lmhealth.org Thu Apr 9 06:51:22 2015 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Apr 9 06:51:35 2015 Subject: [Histonet] Histology position in Newark, Ohio Message-ID: Sorry. Not sure why the link didn't work. www.lmhealth.org Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From TMcNemar <@t> lmhealth.org Thu Apr 9 07:06:25 2015 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Apr 9 07:06:40 2015 Subject: [Histonet] Well. Don't I look like a dummy! Message-ID: Sorry that it took me 3 emails to get the link right..... Licking Memorial Hospital has an opening for a full time certified histology tech. Dayshift position, 7:30am to 4:00pm, Monday through Friday with no weekends or holidays. If interested check us out and apply online at: http://www.lmhealth.org/ Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From jerrysedgewick <@t> gmail.com Thu Apr 9 09:54:57 2015 From: jerrysedgewick <@t> gmail.com (J. Sedgewick) Date: Thu Apr 9 09:55:11 2015 Subject: [Histonet] Auto-Figures Message-ID: <8BC84A2BD2C447F599A1CF478D5F7CC9@sedge> If anyone in this group has to make figures, or if you know others who do, you might be interested in FREE add-ons to Photoshop. You can get a figure layout add-on that will reduce the time it takes to make a figure into seconds. Other add-ons include scripts that ensure you are recording steps in Photoshop for an audit trail (good for GLP), and add-ons that include dozens of methods for post-processing. Go to http://www.imagingandanalysis.com. Best, Jerry Sedgewick From deb.vaneyck <@t> phci.org Thu Apr 9 12:20:58 2015 From: deb.vaneyck <@t> phci.org (Van Eyck, Deb) Date: Thu Apr 9 12:21:07 2015 Subject: [Histonet] Does anyone know why HP35 ultra High profile mocrotome blades no longeer available? Message-ID: Does anyone know why the HP35 ultra high profile microtome blade is no longer available? It seems like it was very widely used and well liked? Thanks Deb ______________________________________________________________________ This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ______________________________________________________________________ From mills <@t> 3scan.com Thu Apr 9 13:26:02 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Thu Apr 9 13:26:26 2015 Subject: [Histonet] Maximum size of tissue for paraffin processing Message-ID: Hi there wonderful Histonet people, Has anyone seen any studies on the maximum size of tissue that can be paraffin (or resin) processed. I am not talking about the size that can fit in a tissue cassette, but, for example, entire pig bladder processing. I realize it would include extended processing time, temp, vacuum, agitation so imagine that none of these factors were limiting as I shall be using an automated tissue processor. I also realize that this is going to be tissue dependent. I was hoping there were already some studies in this area that I am not finding in both google and pubmed searches. thanks, in advance, for any links you can point me to, yours, Caroline -- Caroline Miller Director of Histology 3Scan.com 415 2187297 From rosenfeldtek <@t> hotmail.com Thu Apr 9 14:30:38 2015 From: rosenfeldtek <@t> hotmail.com (Jerry Ricks) Date: Thu Apr 9 14:30:44 2015 Subject: [Histonet] Movat Stain Ferric Chloride Solution Color? Message-ID: I do a lot of Movat Pentachrome stains. Every once in a while something goes wrong! I just made a solution of Ferric Chloride as follows: Dissolve 12.4 grams Ferric Chloride Hexahydrate in 500 ml dH2O. THen add 5.0 ml of concentrated Hydrochloric acid. What is troubling me is that the solution usually starts of brownish, then turns clear yellow after I add the HCl. This time is stayed brownish and didn't turn yellow. Seems like 15 years ago we did a Movat where someone hadn't added the HCL to the solution, it staed brown, and the Weigarts part of the stain didn't work. Any ideas? I'm sure I added the HCl. Jerry L Ricks Research Scientist University of Washington Department of Pathology From liz <@t> premierlab.com Thu Apr 9 14:44:05 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 9 14:44:12 2015 Subject: [Histonet] Maximum size of tissue for paraffin processing In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79F180BC@SBS2K8.premierlab.local> We processed tissues as large as elk and lion vocal cords - 2 cm x 5 cm. You just need to adjust the time accordingly. Very large samples we would process manually through graded alcohols and then the last absolute, xylene and paraffin would be run on the tissue processor, we have programs that range from 6 to 24 hours a station depending upon tissue size. You can run the entire process cycle on a tissue processor its just that we did not want to hang up one of the processors for a week on one study. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Miller Sent: Thursday, April 09, 2015 12:26 PM To: Histonet@Lists. Edu Subject: [Histonet] Maximum size of tissue for paraffin processing Hi there wonderful Histonet people, Has anyone seen any studies on the maximum size of tissue that can be paraffin (or resin) processed. I am not talking about the size that can fit in a tissue cassette, but, for example, entire pig bladder processing. I realize it would include extended processing time, temp, vacuum, agitation so imagine that none of these factors were limiting as I shall be using an automated tissue processor. I also realize that this is going to be tissue dependent. I was hoping there were already some studies in this area that I am not finding in both google and pubmed searches. thanks, in advance, for any links you can point me to, yours, Caroline -- Caroline Miller Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Apr 9 15:04:09 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 9 15:07:07 2015 Subject: [Histonet] Maximum size of tissue for paraffin processing In-Reply-To: References: Message-ID: <695623136.3052751.1428609849502.JavaMail.yahoo@mail.yahoo.com> I am not aware of any such studies [dealing with specimen sizes] but it is a known fact since?early XXth century that large specimens [such as complete brain slices] are known to be processed and yes, they require extended dehydrtaion ? clearing ? infiltration but not much more than usual IF the slices are thin enough, and yes, automated tissue processors can be used?[I have done them for brain and whole prostates].Ren? J.? On Thursday, April 9, 2015 2:26 PM, Caroline Miller wrote: Hi there wonderful Histonet people, Has anyone seen any studies on the maximum size of tissue that can be paraffin (or resin) processed. I am not talking about the size that can fit in a tissue cassette, but, for example, entire pig bladder processing. I realize it would include extended processing time, temp, vacuum, agitation so imagine that none of these factors were limiting as I shall be using an automated tissue processor. I also realize that this is going to be tissue dependent. I was hoping there were already some studies in this area that I am not finding in both google and pubmed searches. thanks, in advance, for any links you can point me to, yours, Caroline -- Caroline Miller Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Thursday, April 9, 2015 2:26 PM, Caroline Miller wrote: Hi there wonderful Histonet people, Has anyone seen any studies on the maximum size of tissue that can be paraffin (or resin) processed. I am not talking about the size that can fit in a tissue cassette, but, for example, entire pig bladder processing. I realize it would include extended processing time, temp, vacuum, agitation so imagine that none of these factors were limiting as I shall be using an automated tissue processor. I also realize that this is going to be tissue dependent. I was hoping there were already some studies in this area that I am not finding in both google and pubmed searches. thanks, in advance, for any links you can point me to, yours, Caroline -- Caroline Miller Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruio7 <@t> hotmail.com Thu Apr 9 15:23:01 2015 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Thu Apr 9 15:23:06 2015 Subject: [Histonet] soften beak Message-ID: Hi, I am just wondering what you would recommend to soften avian beak for paraffin processing. I found a protocol that KOH could be used to soften keratin in nail, however, I have not been able to find that this method is applicable specific to beak. Another concern is if i use KOH for an entire head, does KOH affect (e.g. damage) soft tissues? Thank you in advance, rui From robert.jacox <@t> thermofisher.com Thu Apr 9 15:55:26 2015 From: robert.jacox <@t> thermofisher.com (Jacox, Robert A.) Date: Thu Apr 9 15:55:36 2015 Subject: [Histonet] Job Opening: Histo Tech/quality assurance analyst position located in Kalamazoo MI Message-ID: <7B380BF8E6354F4994F20E095D43F1710B0B84C944@USPHO-MXVS01.amer.thermo.com> ThermoFisher Scientific currently has an opening for a Histotech focused on quality assurance in Kalamazoo MI. For addition information please send your resume or contact Mandy Roschek at (269) 544-5718 or email mandy.roschek@thermofisher.com. Robert Jacox Manager, Global Tactical Marketing Anatomic Pathology Thermo Fisher Scientific Tel: 269-544-5651 l Mobile: 269-598-0747 robert.jacox@thermofisher.com l www.thermoscientific.com From andreahooper <@t> rocketmail.com Fri Apr 10 06:21:20 2015 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Fri Apr 10 06:21:22 2015 Subject: [Histonet] Mouse CD8 for FFPE Message-ID: <2388DC2C-08A2-4FBC-8DF6-4339CC374989@rocketmail.com> Does anyone know of a good clone for mouse CD8 for FFPE? Many thanks! Andrea From Kimberly <@t> animalreferencepathology.com Fri Apr 10 07:02:31 2015 From: Kimberly <@t> animalreferencepathology.com (Kimberly Marshall) Date: Fri Apr 10 07:02:40 2015 Subject: [Histonet] Microwave processing Message-ID: <1428667353541.24604@animalreferencepathology.com> ?Happy Friday Histo peeps. I have an microwave issue and want to ask about it. I have recently started a Mid day run here in my lab that consist of the smaller specimen, skin punch, gingiva, just the smaller lumps and bumps from folks pets. I have a very inconstant issue with the skins not processing well. Is there anyone out there using Labpulse microwave processors that would share their protocol? As well the pathologist say the staining is too pink on these specimens. Its a automated stainer so there are no changes. I am thinking processing, so again if you have a separate staining protocol used for microwave specimens please share. Thanks in advance for the help Kimberly Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg@01CF8F87.A0BD4830] From ramakrishna.samala <@t> ttuhsc.edu Fri Apr 10 09:00:27 2015 From: ramakrishna.samala <@t> ttuhsc.edu (Samala, Ramakrishna) Date: Fri Apr 10 09:00:42 2015 Subject: [Histonet] Paraffin embedding of whole mouse brain Message-ID: Hello Histonet! I would like to paraffin embed whole mouse brain to obtain coronal sections. I spoke with customer support teams of several companies, I could able to get 10 mm deep molds, but the mouse brain is about 13 mm height. So, any help in this regard is much appreciated. Sincerely Ramakrishna Samala, Ph.D. Senior Research Associate Amarillo Research Building, School of Pharmacy, Texas Tech University Health Sciences Center 1406 S. Coulter Amarillo, TX 79106 The outcome of any serious research can only be to make two questions grow where only one grew before ?Thorstein Veblen From tpodawiltz <@t> lrgh.org Fri Apr 10 09:03:47 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Apr 10 09:03:59 2015 Subject: [Histonet] Referral/Second opinion Cases. Message-ID: <38667E7FB77ECD4E91BFAEB8D9863863261678791A@LRGHEXVS1.practice.lrgh.org> I was asked by my Director about any cases that are send out for a second opinion and do you bill the patient for them. I feel that if one of our pathologist send a case out, we don't bill, however if the request comes from the PCP you should. So that is the question he asked what do other facilities do? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Toni.Rathborne <@t> rwjuh.edu Fri Apr 10 09:16:03 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Fri Apr 10 09:16:09 2015 Subject: [Histonet] RE: Referral/Second opinion Cases. In-Reply-To: <38667E7FB77ECD4E91BFAEB8D9863863261678791A@LRGHEXVS1.practice.lrgh.org> References: <38667E7FB77ECD4E91BFAEB8D9863863261678791A@LRGHEXVS1.practice.lrgh.org> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F75C81@vap1013.win.rwjuh.edu> That is what we do too, whether the request comes from the PCP or the patient, they are billed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, April 10, 2015 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Referral/Second opinion Cases. I was asked by my Director about any cases that are send out for a second opinion and do you bill the patient for them. I feel that if one of our pathologist send a case out, we don't bill, however if the request comes from the PCP you should. So that is the question he asked what do other facilities do? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGleiberman <@t> buffalobiolabs.com Fri Apr 10 09:16:52 2015 From: AGleiberman <@t> buffalobiolabs.com (Anatoli Gleiberman) Date: Fri Apr 10 09:17:02 2015 Subject: [Histonet] RE: Paraffin embedding of whole mouse brain In-Reply-To: References: Message-ID: You can make your own molds from thick alum foil any deep you need Anatoli Gleiberman, Ph.D. Director of Histopathology Buffalo Biolabs LLC 73 High Street Buffalo, NY 14203 Phone: 716-849-6810x354 e-mail: agleiberman@buffalobiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Samala, Ramakrishna Sent: Friday, April 10, 2015 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin embedding of whole mouse brain Hello Histonet! I would like to paraffin embed whole mouse brain to obtain coronal sections. I spoke with customer support teams of several companies, I could able to get 10 mm deep molds, but the mouse brain is about 13 mm height. So, any help in this regard is much appreciated. Sincerely Ramakrishna Samala, Ph.D. Senior Research Associate Amarillo Research Building, School of Pharmacy, Texas Tech University Health Sciences Center 1406 S. Coulter Amarillo, TX 79106 The outcome of any serious research can only be to make two questions grow where only one grew before -Thorstein Veblen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DEBORAH_ELLENBURG <@t> bshsi.org Fri Apr 10 09:38:16 2015 From: DEBORAH_ELLENBURG <@t> bshsi.org (Ellenburg, Deborah) Date: Fri Apr 10 09:38:55 2015 Subject: [Histonet] RE: Referral/Second opinion Cases. In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24F75C81@vap1013.win.rwjuh.edu> References: <38667E7FB77ECD4E91BFAEB8D9863863261678791A@LRGHEXVS1.practice.lrgh.org> <59E09A4EFBD3F349BD75FDAE8AFB0F24F75C81@vap1013.win.rwjuh.edu> Message-ID: This seems to be a popular question. Who gets billed if the request for the second opinion is requested by the??? * Pathologist____________________________________ * Patient_______________________________________ * Ordering physician______________________________ * Oncologists____________________________________ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, April 10, 2015 10:16 AM To: 'Podawiltz, Thomas'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. That is what we do too, whether the request comes from the PCP or the patient, they are billed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, April 10, 2015 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Referral/Second opinion Cases. I was asked by my Director about any cases that are send out for a second opinion and do you bill the patient for them. I feel that if one of our pathologist send a case out, we don't bill, however if the request comes from the PCP you should. So that is the question he asked what do other facilities do? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From Joyce.Weems <@t> emoryhealthcare.org Fri Apr 10 09:46:24 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Apr 10 09:46:30 2015 Subject: [Histonet] RE: Referral/Second opinion Cases. In-Reply-To: References: <38667E7FB77ECD4E91BFAEB8D9863863261678791A@LRGHEXVS1.practice.lrgh.org> <59E09A4EFBD3F349BD75FDAE8AFB0F24F75C81@vap1013.win.rwjuh.edu> Message-ID: Who gets billed if the request for the second opinion is requested by the??? * Pathologist_____Pathologist if insurance does not cover * Patient___Patient if insurance does not cover or client only billed * Ordering physician_____ Patient if insurance does not cover or client only billed * Oncologists________ Patient if insurance does not cover or client only billed Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellenburg, Deborah Sent: Friday, April 10, 2015 10:38 AM To: Rathborne, Toni; 'Podawiltz, Thomas'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. This seems to be a popular question. Who gets billed if the request for the second opinion is requested by the??? * Pathologist____________________________________ * Patient_______________________________________ * Ordering physician______________________________ * Oncologists____________________________________ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, April 10, 2015 10:16 AM To: 'Podawiltz, Thomas'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. That is what we do too, whether the request comes from the PCP or the patient, they are billed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, April 10, 2015 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Referral/Second opinion Cases. I was asked by my Director about any cases that are send out for a second opinion and do you bill the patient for them. I feel that if one of our pathologist send a case out, we don't bill, however if the request comes from the PCP you should. So that is the question he asked what do other facilities do? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Bonnie.Whitaker <@t> osumc.edu Fri Apr 10 09:49:00 2015 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Fri Apr 10 09:49:14 2015 Subject: [Histonet] RE: Referral/Second opinion Cases. In-Reply-To: References: <38667E7FB77ECD4E91BFAEB8D9863863261678791A@LRGHEXVS1.practice.lrgh.org> <59E09A4EFBD3F349BD75FDAE8AFB0F24F75C81@vap1013.win.rwjuh.edu> Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E14E232FD@EXMBOX-VP05.OSUMC.EDU> In our institution, we (pathology) pay for anything requested by someone in our department. We provide billing information, along with the slides, when we send out a consult requested by anyone else. Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellenburg, Deborah Sent: Friday, April 10, 2015 10:38 AM To: Rathborne, Toni; 'Podawiltz, Thomas'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. This seems to be a popular question. Who gets billed if the request for the second opinion is requested by the??? * Pathologist____________________________________ * Patient_______________________________________ * Ordering physician______________________________ * Oncologists____________________________________ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, April 10, 2015 10:16 AM To: 'Podawiltz, Thomas'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. That is what we do too, whether the request comes from the PCP or the patient, they are billed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, April 10, 2015 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Referral/Second opinion Cases. I was asked by my Director about any cases that are send out for a second opinion and do you bill the patient for them. I feel that if one of our pathologist send a case out, we don't bill, however if the request comes from the PCP you should. So that is the question he asked what do other facilities do? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=fkbzppRjUfybk__CHkscNp_lHgAu0_b11vghraVH5QQ&s=X_1DcWkxCTW3wkf97nUNkD0axLTFKqFuwlGlsU8Yo5s&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=fkbzppRjUfybk__CHkscNp_lHgAu0_b11vghraVH5QQ&s=X_1DcWkxCTW3wkf97nUNkD0axLTFKqFuwlGlsU8Yo5s&e= ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=fkbzppRjUfybk__CHkscNp_lHgAu0_b11vghraVH5QQ&s=X_1DcWkxCTW3wkf97nUNkD0axLTFKqFuwlGlsU8Yo5s&e= From LRaff <@t> uropartners.com Fri Apr 10 09:53:24 2015 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Fri Apr 10 09:53:46 2015 Subject: [Histonet] RE: Referral/Second opinion Cases. In-Reply-To: <6B106EE8C8AAEF449DEA97921DEC11670E14E232FD@EXMBOX-VP05.OSUMC.EDU> References: <38667E7FB77ECD4E91BFAEB8D9863863261678791A@LRGHEXVS1.practice.lrgh.org><59E09A4EFBD3F349BD75FDAE8AFB0F24F75C81@vap1013.win.rwjuh.edu> <6B106EE8C8AAEF449DEA97921DEC11670E14E232FD@EXMBOX-VP05.OSUMC.EDU> Message-ID: <0A05EC63892F834395DCC6047CAD0C5B9B63B2@UPHQMSX01.uropartners.local> Same for us as Bonnie describes. And we send out A LOT of second opinion requests since that is close to becoming standard of care in prostate cancer. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Whitaker, Bonnie Sent: Friday, April 10, 2015 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. In our institution, we (pathology) pay for anything requested by someone in our department. We provide billing information, along with the slides, when we send out a consult requested by anyone else. Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellenburg, Deborah Sent: Friday, April 10, 2015 10:38 AM To: Rathborne, Toni; 'Podawiltz, Thomas'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. This seems to be a popular question. Who gets billed if the request for the second opinion is requested by the??? * Pathologist____________________________________ * Patient_______________________________________ * Ordering physician______________________________ * Oncologists____________________________________ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, April 10, 2015 10:16 AM To: 'Podawiltz, Thomas'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. That is what we do too, whether the request comes from the PCP or the patient, they are billed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, April 10, 2015 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Referral/Second opinion Cases. I was asked by my Director about any cases that are send out for a second opinion and do you bill the patient for them. I feel that if one of our pathologist send a case out, we don't bill, however if the request comes from the PCP you should. So that is the question he asked what do other facilities do? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern .edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPE vxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=fkbzppRjUf ybk__CHkscNp_lHgAu0_b11vghraVH5QQ&s=X_1DcWkxCTW3wkf97nUNkD0axLTFKqFuwlGl sU8Yo5s&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern .edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPE vxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=fkbzppRjUf ybk__CHkscNp_lHgAu0_b11vghraVH5QQ&s=X_1DcWkxCTW3wkf97nUNkD0axLTFKqFuwlGl sU8Yo5s&e= ________________________________________________________________________ ________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________ ________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern .edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPE vxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=fkbzppRjUf ybk__CHkscNp_lHgAu0_b11vghraVH5QQ&s=X_1DcWkxCTW3wkf97nUNkD0axLTFKqFuwlGl sU8Yo5s&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Fri Apr 10 09:54:37 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Apr 10 09:54:43 2015 Subject: [Histonet] RE: Referral/Second opinion Cases. In-Reply-To: <6B106EE8C8AAEF449DEA97921DEC11670E14E232FD@EXMBOX-VP05.OSUMC.EDU> References: <38667E7FB77ECD4E91BFAEB8D9863863261678791A@LRGHEXVS1.practice.lrgh.org> <59E09A4EFBD3F349BD75FDAE8AFB0F24F75C81@vap1013.win.rwjuh.edu> <6B106EE8C8AAEF449DEA97921DEC11670E14E232FD@EXMBOX-VP05.OSUMC.EDU> Message-ID: <469a512c114f4804836d41755988d5d8@MAIL13M2N2.ad.uams.edu> Same here. If we are requesting for internal use we pay for it. Anything outside of our facility requested will get the cost for all services performed for any phase in the overall AP area. (Slides, blocks, stains or special procedures etc.) Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Whitaker, Bonnie Sent: Friday, April 10, 2015 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. In our institution, we (pathology) pay for anything requested by someone in our department. We provide billing information, along with the slides, when we send out a consult requested by anyone else. Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellenburg, Deborah Sent: Friday, April 10, 2015 10:38 AM To: Rathborne, Toni; 'Podawiltz, Thomas'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. This seems to be a popular question. Who gets billed if the request for the second opinion is requested by the??? * Pathologist____________________________________ * Patient_______________________________________ * Ordering physician______________________________ * Oncologists____________________________________ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, April 10, 2015 10:16 AM To: 'Podawiltz, Thomas'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Referral/Second opinion Cases. That is what we do too, whether the request comes from the PCP or the patient, they are billed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, April 10, 2015 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Referral/Second opinion Cases. I was asked by my Director about any cases that are send out for a second opinion and do you bill the patient for them. I feel that if one of our pathologist send a case out, we don't bill, however if the request comes from the PCP you should. So that is the question he asked what do other facilities do? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=fkbzppRjUfybk__CHkscNp_lHgAu0_b11vghraVH5QQ&s=X_1DcWkxCTW3wkf97nUNkD0axLTFKqFuwlGlsU8Yo5s&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=fkbzppRjUfybk__CHkscNp_lHgAu0_b11vghraVH5QQ&s=X_1DcWkxCTW3wkf97nUNkD0axLTFKqFuwlGlsU8Yo5s&e= ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=fkbzppRjUfybk__CHkscNp_lHgAu0_b11vghraVH5QQ&s=X_1DcWkxCTW3wkf97nUNkD0axLTFKqFuwlGlsU8Yo5s&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From amurvosh <@t> advancederm.net Fri Apr 10 10:17:47 2015 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Fri Apr 10 10:17:57 2015 Subject: [Histonet] RE: Paraffin embedding of whole mouse brain In-Reply-To: References: Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7A81A36@Exchange.Advancederm.net> I have actually embedded specimens that the PA cut in too thick with the cassette upside down to form a dome, using a lot of paraffin. I don't know if it would work with something so large. You could try one of the deep cassettes I've seen in catalogs with the large mold, if regular cassette is still too small. It's a little crazy, but an option if there are no others. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Samala, Ramakrishna Sent: Friday, April 10, 2015 7:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin embedding of whole mouse brain Hello Histonet! I would like to paraffin embed whole mouse brain to obtain coronal sections. I spoke with customer support teams of several companies, I could able to get 10 mm deep molds, but the mouse brain is about 13 mm height. So, any help in this regard is much appreciated. Sincerely Ramakrishna Samala, Ph.D. Senior Research Associate Amarillo Research Building, School of Pharmacy, Texas Tech University Health Sciences Center 1406 S. Coulter Amarillo, TX 79106 The outcome of any serious research can only be to make two questions grow where only one grew before -Thorstein Veblen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tejohnson <@t> genoptix.com Fri Apr 10 10:24:27 2015 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Fri Apr 10 10:24:37 2015 Subject: [Histonet] IHC Proficiency testing Message-ID: Dear colleagues, For those of you who have very busy IHC laboratories, how do you conduct proficiency testing in your lab that meets CAP requirements? Do you do each active antibody you offer every 6 months, or do you have some other mechanism for showing proficiency? Happy Friday! Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From jkiernan <@t> uwo.ca Fri Apr 10 10:28:31 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Apr 10 10:28:34 2015 Subject: [Histonet] Movat Stain Ferric Chloride Solution Color? In-Reply-To: <73e0def464d8.5527eaee@uwo.ca> References: <7270ffaa3f21.5527e806@uwo.ca> <72e0e35339b8.5527e843@uwo.ca> <7360c72727cb.5527e881@uwo.ca> <73e096284f51.5527e8bf@uwo.ca> <7310d979479.5527e8fd@uwo.ca> <72e0fc9e1735.5527e93c@uwo.ca> <73709e5b7074.5527e97a@uwo.ca> <72d0ba91583b.5527e9b8@uwo.ca> <7320dbf9142.5527e9f6@uwo.ca> <7290d7a05d7f.5527ea35@uwo.ca> <72d08b322a.5527eab0@uwo.ca> <73e0def464d8.5527eaee@uwo.ca> Message-ID: <72e0f8872151.5527a5cf@uwo.ca> Check with a pH meter. The ferric chloride solution in water is close to 0.1M and should have pH=2. 5ml of conc. HCl in 500ml of water is also about 0.1M, with pH=1. The change in colour from orange to yellow is due to further acidification. The acidified (yellow) solution keeps for years, but a pale precipitate forms slowly (weeks to months) in unacidified FeCl3 solutions. My guess is that your supply of conc. HCl is no longer conc. enough to change the colour of the FeCl3. Checking the pH should confirm. Remember that the electrode may take some time (minutes) to equilibrate at low pH. John Kiernan London, Canada = = = On 09/04/15, Jerry Ricks wrote: > I do a lot of Movat Pentachrome stains. Every once in a while something goes wrong! > > I just made a solution of Ferric Chloride as follows: Dissolve 12.4 grams Ferric Chloride Hexahydrate in 500 ml dH2O. THen add 5.0 ml of concentrated Hydrochloric acid. > > What is troubling me is that the solution usually starts of brownish, then turns clear yellow after I add the HCl. This time is stayed brownish and didn't turn yellow. > > Seems like 15 years ago we did a Movat where someone hadn't added the HCL to the solution, it staed brown, and the Weigerts part of the stain didn't work. > > Any ideas? I'm sure I added the HCl. > > > Jerry L Ricks > Research Scientist > University of Washington > Department of Pathology > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Fri Apr 10 10:38:20 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 10 10:38:26 2015 Subject: [Histonet] soften beak In-Reply-To: References: Message-ID: <1485717612.345177.1428680300727.JavaMail.yahoo@mail.yahoo.com> 10% KOH or NaOH for 30 minutes, will soften nails keratin and with longer exposure it will soften an avian beak BUT if the exposure is too prolonged it may affect the microscopic structure of the soft tissues.Exposing the whole head to KOH makes no sense because the bones will not be "soften" and the tissues will be affected. Besides, if you intend to infiltrate and section the whole head, you will have to use, on top of the KOH, an acid decalcifier.Ren? J. On Thursday, April 9, 2015 4:23 PM, Rui TAHARA wrote: Hi, I am just wondering what you would recommend to soften avian beak for paraffin processing. I found a protocol that KOH could be used to soften keratin in nail, however, I have not been able to find that this method is applicable specific to beak. Another concern is if i use KOH for an entire head, does KOH affect (e.g. damage) soft tissues? Thank you in advance, rui ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Fri Apr 10 10:39:16 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Apr 10 10:39:23 2015 Subject: [Histonet] Paraffin embedding of whole mouse brain In-Reply-To: References: Message-ID: <1624513548.932202.1428680356677.JavaMail.zimbra@comcast.net> Hi, could you cut the brain in half (in coronal cut orientation).? Using the great mouse brain molds available you could cut "in half" the brain at exactly the spot you wanted.? So have two 6-7 mm blocks both in coronal sectioning orientation and you can cut as you wish from the two blocks.? Alternatively, on the Allen Brain Atlas page they have coronal sections of mouse brain (132 of them cut at 100um intervals so I guess that is your 132x100=13,200 um- or 13 mm).? That wouldn't be a trade secret if you asked them. ? Ray, Lake Forest Park, WA ----- Original Message ----- From: "Ramakrishna Samala" To: histonet@lists.utsouthwestern.edu Sent: Friday, April 10, 2015 7:00:27 AM Subject: [Histonet] Paraffin embedding of whole mouse brain Hello Histonet! I would like to paraffin embed whole mouse brain to obtain coronal sections. I spoke with customer support teams of several companies, I could able to get 10 mm deep molds, ?but the mouse brain is about 13 mm height. So, any help in this regard is much appreciated. Sincerely Ramakrishna Samala, Ph.D. Senior Research Associate Amarillo Research Building, School of Pharmacy, Texas Tech University Health Sciences Center 1406 S. Coulter Amarillo, TX 79106 The outcome of any serious research can only be to make two questions grow where only one grew before ?Thorstein Veblen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Apr 10 11:17:52 2015 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Fri Apr 10 11:17:57 2015 Subject: [Histonet] Paraffin embedding of whole mouse brain In-Reply-To: References: Message-ID: We use these--22mm deep. They are disposable. You can only get them from Polysciences too. https://www.tedpella.com/Embedding_html/Peel-A-Way_Disposable_Histology_Molds.htm Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Fri, Apr 10, 2015 at 10:00 AM, Samala, Ramakrishna < ramakrishna.samala@ttuhsc.edu> wrote: > Hello Histonet! > > I would like to paraffin embed whole mouse brain to obtain coronal > sections. I spoke with customer support teams of several companies, I could > able to get 10 mm deep molds, but the mouse brain is about 13 mm height. > So, any help in this regard is much appreciated. > > Sincerely > > Ramakrishna Samala, Ph.D. > Senior Research Associate > Amarillo Research Building, School of Pharmacy, Texas Tech University > Health Sciences Center > 1406 S. Coulter > Amarillo, TX 79106 > > The outcome of any serious research can only be to make two questions grow > where only one grew before ?Thorstein Veblen > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rmire <@t> cvpath.org Fri Apr 10 11:25:00 2015 From: rmire <@t> cvpath.org (Ronda Mire) Date: Fri Apr 10 11:25:11 2015 Subject: [Histonet] IHC Proficiency testing In-Reply-To: References: Message-ID: Check the CAP PT menu. I have in the past used CAP proficiency surveys for general IHC as well as ER/PR. Happy Friday Ronda Mire > On Apr 10, 2015, at 11:24 AM, Teri Johnson wrote: > > Dear colleagues, > > For those of you who have very busy IHC laboratories, how do you conduct proficiency testing in your lab that meets CAP requirements? Do you do each active antibody you offer every 6 months, or do you have some other mechanism for showing proficiency? > > Happy Friday! > > Teri Johnson, HT(ASCP)QIHC > Manager Clinical Trial Testing > Genoptix, Inc. > SAN5, Rm. 2005 > 760.516.5954 (office) > 760.516.6201 (fax) > > > ________________________________ > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From catherinesimonson <@t> gmail.com Fri Apr 10 12:38:28 2015 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Fri Apr 10 12:38:30 2015 Subject: [Histonet] RE: whole mouse brains Message-ID: Hey there! I embed whole mouse brains on a regular basis. First, process on a longer schedule (about 1 to 1.5 hours per station, really. Otherwise they are under processed and will not cut well and you will have problems getting them to stay on the slides during staining). Use the deep molds. Keep in mind that the tissue will shrink (about 20 - 30 %) during processing so they WILL fit for coronal sections. If need be, trim some of the olfactory bulbs off (you probably would be facing those off on the microtome anyhow). Hope this helps, Catherine Simonson, HT (ASCP) The Jackson Laboratory Bar Harbor, ME From s_musat <@t> yahoo.com Fri Apr 10 14:52:25 2015 From: s_musat <@t> yahoo.com (Sorin Musat) Date: Fri Apr 10 14:52:40 2015 Subject: [Histonet] Paraffin embedding of whole mouse brain In-Reply-To: References: Message-ID: <1355934923.505938.1428695545755.JavaMail.yahoo@mail.yahoo.com> Hi Samala I did some 10 years ago coronal sections on rat brains (cerebellum was not interesting to my client - he needed BrdU staining, so it was discarded). The resulting blocks were approx 2 cm tall. The only option was to make my own X-tra deep molds (heavy gauge aluminum foil and aluminum tape did the trick). The blocks were very tall but by shaping them carefully (wider base) and sectioning very slowly I was able to achieve serial sectioning (saved 3 sections every 50 microns step). Sectioning the 125 brains to completion took me almost a month and a damaged wrist. But the project turned out OK.So, I think you could make your own molds fairly easily. Just make sure processing is done well (gently), the blocks are properly shaped and the chuck is keeping the block vibration-free. Keep a rim of paraffin around the brain, 5-6 mm at a minimum and lower the temperature of the flotation bath. Good Luck! Sorin MusatThemis PathologyBucharest, RomaniaformerlyHistoBest Inc.Edmonton, Alberta? From: Emily Brown To: "Samala, Ramakrishna" Cc: "histonet@lists.utsouthwestern.edu" Sent: Friday, April 10, 2015 7:17 PM Subject: Re: [Histonet] Paraffin embedding of whole mouse brain We use these--22mm deep.? They are disposable.? You can only get them from Polysciences too. https://www.tedpella.com/Embedding_html/Peel-A-Way_Disposable_Histology_Molds.htm Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Fri, Apr 10, 2015 at 10:00 AM, Samala, Ramakrishna < ramakrishna.samala@ttuhsc.edu> wrote: > Hello Histonet! > > I would like to paraffin embed whole mouse brain to obtain coronal > sections. I spoke with customer support teams of several companies, I could > able to get 10 mm deep molds,? but the mouse brain is about 13 mm height. > So, any help in this regard is much appreciated. > > Sincerely > > Ramakrishna Samala, Ph.D. > Senior Research Associate > Amarillo Research Building, School of Pharmacy, Texas Tech University > Health Sciences Center > 1406 S. Coulter > Amarillo, TX 79106 > > The outcome of any serious research can only be to make two questions grow > where only one grew before ?Thorstein Veblen > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade <@t> gmail.com Fri Apr 10 15:00:55 2015 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Apr 10 15:00:59 2015 Subject: [Histonet] formic acid treatment and alpha-synuclein staining Message-ID: Hello, I'm do some research on alpha-synucleinopathies and how to stain them. I am reading, from the Braak et al papers, that the slides need be treated for 10 minutes with formic acid. The method is largely absent. I'm a biochemist so I understand the formic acid is denaturing and unfolding the aggregates and exposing the epitopes. Would this effect the ability to label other epitopes such as plaques and tangles, tyrosine-hydroxylase, FOX2A etc..? Does anyone have any experience in double-labeling sections for Lewy Bodies and other proteins? In the same paper an alternative method of a 48 hr incubation on the slide is suggested. Details of the staining buffer are absent but I'm hunting them down and expect to find something like Triton X-100 in it which will again unfold the aggregate (a little). Does anyone have a reliable protocol to share? I'm now several references deep* from my original article and am still trying to find the paper that describes the method. Thanks -- Tyrone Genade *Back in my Biochemistry days we played a game: Hunting for Bradford. Essential, select a paper at random, see if it uses the Bradford Assay and find out how many papers you had to read before arriving at the original paper by Bradford. My then Prof held the record at some 30+ papers... Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From JWatson <@t> gnf.org Fri Apr 10 15:33:53 2015 From: JWatson <@t> gnf.org (James Watson) Date: Fri Apr 10 15:33:58 2015 Subject: [Histonet] RE: whole mouse brains In-Reply-To: References: Message-ID: We also have embed mouse brains for coronal sections routinely in deep molds, yes it can be close at times. With rat brains we actually embedded in deep molds with the Mega cassette upside down so part of the brain went above the mold and into the cassette. If we needed to go through the whole brain once we got near the cassette we would re-embed the brain so the reminder was out of the cassette. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Simonson Sent: Friday, April 10, 2015 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole mouse brains Hey there! I embed whole mouse brains on a regular basis. First, process on a longer schedule (about 1 to 1.5 hours per station, really. Otherwise they are under processed and will not cut well and you will have problems getting them to stay on the slides during staining). Use the deep molds. Keep in mind that the tissue will shrink (about 20 - 30 %) during processing so they WILL fit for coronal sections. If need be, trim some of the olfactory bulbs off (you probably would be facing those off on the microtome anyhow). Hope this helps, Catherine Simonson, HT (ASCP) The Jackson Laboratory Bar Harbor, ME _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From blayjorge <@t> gmail.com Sun Apr 12 08:45:20 2015 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Sun Apr 12 08:45:25 2015 Subject: [Histonet] Pigs as models of human biology Message-ID: Dear Histonetters: I often wonder what are the reasons why pigs seem to be used so often in studies of human physiology. For phylogenetic reasons, I would have thought chimps would be the preferred choice. Is it because of humane, $, or are there other considerations? Thank you. If you know, please send me an email to: blayjorge@gmail.com Sincerely (and apologies if you have received this message more than once), Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From jclark <@t> pcnm.com Sun Apr 12 13:23:51 2015 From: jclark <@t> pcnm.com (Joanne Clark) Date: Sun Apr 12 13:23:55 2015 Subject: [Histonet] Re: Histonet Digest, Vol 137, Issue 15 In-Reply-To: <8d56e2fe-85ae-4bb0-96f9-089a6f7c8f32@S10HUB004.SH10.lan> References: <8d56e2fe-85ae-4bb0-96f9-089a6f7c8f32@S10HUB004.SH10.lan> Message-ID: <314E2339-571E-475E-8A49-BFD44333F072@pcnm.com> She can be here in two weeks. Sent from my iPhone > On Apr 12, 2015, at 11:01 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://cp.mcafee.com/d/avndxMwcCQm4TDDTHzDztPqdQnCnNPUVYSzt5VBYsUCyrhKyYO-ev7e6QrLIe8IcTd79IhIvIiEamJ1nBPqREaYKrZ1ztjvvW_9L6zBdAQsCzDHTbFThsVYsed7arb_bnjIyyHtdDBgY-F6lK1FJ4SYrLRQkhPz0WXVEVdTdw0PVkDjCVQGTcCo-A_z3LgrMjlS67OFek7qUjBitgqr9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJyXzNIbOFeEdd40t8zZ9w6q80PkYQg8Izw3d40rizvCy15YSOMrgo0Td3SY > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Pigs as models of human biology (Jorge A. Santiago-Blay) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 12 Apr 2015 09:45:20 -0400 > From: "Jorge A. Santiago-Blay" > Subject: [Histonet] Pigs as models of human biology > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Dear Histonetters: > > > I often wonder what are the reasons why pigs seem to be used so often in > studies of human physiology. For phylogenetic reasons, I would have thought > chimps would be the preferred choice. Is it because of humane, $, or are > there other considerations? Thank you. > > > > If you know, please send me an email to: blayjorge@gmail.com > > > > Sincerely (and apologies if you have received this message more than once), > > > > Jorge > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > > 1. Positive experiences for authors of papers published in *LEB* > http://cp.mcafee.com/d/1jWVIp6jqb2rPPXRNPNKVJ6WbPbUVYs-rhKyYO-esjhdEThupv7fzD3qdTS74m6rCzAS8SfS9k5bmwHOVJqQ5und-wNKFLLZvATzhOCOqejhPRXBQXEKs-e76zBdB_BHFShhlKCPOEuvkzaT0QSCrudTWWa8VNwttYQsCXCM0p0_iOPp0-l2vNFO-j-AVaRxbjWor9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJyXzNIbOFeEdd40t8zZ9w6q80PkYQg8Izw3d40rizvCy15YSOMrkCZ2 > > 2. Free examples of papers published in *LEB*: > http://cp.mcafee.com/d/k-Kr6jqb2rPPXRNPNKVJ6WbPbUVYs-rhKyYO-esjhdEThupv7fzD3qdTS74m6rCzAS8SfS9k5bmwHOVJqQ5und-wNKFLLZvATzhOCOqejhPRXBQXEKs-e76zBdB_BHFShhlKCPOEuvkzaT0QSOrudTWWa8VNwttYQsCXCM0p0_iOPp0-l2vNFO-mSvAqNboJ7PcGJQbOFhLWor9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJyXzNIbOFeEdd40t8zZ9w6q80PkYQg8Izw3d40rizvCy15YSOMrT2tf. > > 3. *Guidelines for Authors* and page charges of *LEB*: > http://cp.mcafee.com/d/avndy0Ad1MOrhojuuvuKeudTdEThupv7fzDPqdQnCnNPyq9J6WbPbUVYsUrhK-MUyMPsQsCN6N-NawFqQ5undHmwHOVLQ6dRdZ_HYCYqekSjhOqeuLsKDt5PDNMUQsFILYJteOaaJQSul3PWApmU6CS3rNK_nhh7ec3HLCzATsS0387Wmmr87OEj-denPoS9Oc_j3pel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdInsudxul9R1FEw3F4vFc0Ph06qDCy15As0pEw3qkrYQg8LCSm3sTnDDp-e *.* > > 4. Want to subscribe to *LEB*? http://cp.mcafee.com/d/avndxNJ5xdVVZWUVUTsSzt5VBYs-evdEThupv7e9ECQrELcLzDNPxJ6XX3yb3dPhOr4r7X4G2BHglVsSJq2LbC_goTkTT-LOrNEVjpd79EVWZOWtQnev73zhOCO_ORQX8EGTjpVkffGhBrwqrvdL6XZt54sUMeK-qejtPo0cwvFppIwvaxfUQVvadAamz9qsGMFxIDaAWwQKgGT2TQ1hYGjFPsWlrCjcvivNxTEdCSbKf6MLaAWwQQg1QyfQC0pEw3djPh0yOe0cQg1Jad-q84nPrb1Ie9qtDdO > > > http://cp.mcafee.com/d/5fHCNAq3x8SyMCYY-ZssYrKrhKyYO-ev7fCQrELcLzD4QjqdQnCnNPUVMSztZxN5xCVEVdydzZyl1iREaYKrmJ1nBPvEcrGrX_nVdUQsFICzAQsZuVteWbDfzxNEVjpvVqWtAklrFIYG7DR8OJMddK6Tzt-Kyyeso7nvd79KVI06gfQI8w0vUNma2QvFjBYdAVkDk6BO5mUm-wafBiterDiHsOpzWj-ceZ1ISNtNUS5VkDk6Cy0eAh-AM3d40pGuq84mhM1Cy0dFhLPh0y-rpodPZkVyc_Y > http://cp.mcafee.com/d/FZsS86Qm4TDDTHzDztPqdQnCnNPUVYSzt5VBYsUCyrhKyYO-ev7e6QrLIe8IcTd79IhIvIiEamJ1nBPqREaYKrZ1ztjvvW_9L6zBdAQsCzDHTbFThsVYsed7arb_bnjIyyHtdDBgY-F6lK1FIsrudTWWa8VNwttYQsCXCM0qSD_kxaFZi9rGiXQ6YFfnnsB12hCi3uDQOCMDbidi1-BKIWGr9OFeEdbAaJMJZ0kvaAWsTeBmVAP7QDYotW3pJyXzNIbOFeEdd40t8zZ9w6q80PkYQg8Izw3d40rizvCy15YSOMrgNgo > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://cp.mcafee.com/d/2DRPos83gwrhojuuvuKeudTdEThupv7fzDPqdQnCnNPyq9J6WbPbUVYsUrhK-MUyMPsQsCN6N-NawFqQ5undHmwHOVLQ6dRdZ_HYCYqekSjhOqeuLsKDt5PDNMUQsFILYJteOaaJQSul3PWApmU6CTzrNK_nhh7ec3HLCzATsS03fBiterDiHsOpzWj-ceZ1L1dnoovaAVgtHxel9R1FIDaAWwQKgGT2TQ1hYGjFPsWlrCjcvivNxTEdCSbKf6MLaAWwQQg1QyfQC0pEw3djPh0yOe0cQg1Jad-q84nPrb1J67KLKDS2LLj > > End of Histonet Digest, Vol 137, Issue 15 > ***************************************** Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From abtdhu <@t> gmail.com Mon Apr 13 08:45:51 2015 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Mon Apr 13 08:45:55 2015 Subject: [Histonet] formic acid treatment and alpha-synuclein staining (Tyrone Genade) Message-ID: > > I used 88% formic acid in Dwater to pretreatment of mouse tissue slides > before beta-Amyloid and Anti-Glial Fibrillary Acidic Protein (GFAP) > antibodies IHC. 10 minutes in formic acid with gental shaking before > blocking step. You may also check this paper. Acta Neuropathol. 2000 Mar;99(3):296-304. C-terminal alpha-synuclein immunoreactivity in structures other than Lewy bodies in neurodegenerativedisorders. Takeda A 1, Hashimoto M , Mallory M , Sundsumo M , Hansen L , Masliah E . Hope this wil help. Dorothy Hu HSDM > Message: 3 > Date: Fri, 10 Apr 2015 15:00:55 -0500 > From: Tyrone Genade > Subject: [Histonet] formic acid treatment and alpha-synuclein staining > To: histonet > Message-ID: > < > CAEYEE3mA4XHP0_6bRSQZqPuJSoHi15vdNZN+VkBrBB2PFWS4BQ@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello, > > I'm do some research on alpha-synucleinopathies and how to stain them. I am > reading, from the Braak et al papers, that the slides need be treated for > 10 minutes with formic acid. The method is largely absent. I'm a biochemist > so I understand the formic acid is denaturing and unfolding the aggregates > and exposing the epitopes. Would this effect the ability to label other > epitopes such as plaques and tangles, tyrosine-hydroxylase, FOX2A etc..? > Does anyone have any experience in double-labeling sections for Lewy Bodies > and other proteins? > > In the same paper an alternative method of a 48 hr incubation on the slide > is suggested. Details of the staining buffer are absent but I'm hunting > them down and expect to find something like Triton X-100 in it which will > again unfold the aggregate (a little). > > Does anyone have a reliable protocol to share? I'm now several references > deep* from my original article and am still trying to find the paper that > describes the method. > > Thanks > -- > Tyrone Genade > > *Back in my Biochemistry days we played a game: Hunting for Bradford. > Essential, select a paper at random, see if it uses the Bradford Assay and > find out how many papers you had to read before arriving at the original > paper by Bradford. My then Prof held the record at some 30+ papers... > > Orange City, Iowa > tel: (+1) 712 230 4101 > http://tgenade.freeshell.org > > ******************************************************************************** > Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. > To find out how to receive this FREE gift visit http://www.alpha.org. > > From catherinesimonson <@t> gmail.com Mon Apr 13 09:37:46 2015 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Mon Apr 13 09:37:52 2015 Subject: [Histonet] RE: whole mouse brains In-Reply-To: <43D1B55674EC2244A707ACE779624362DB9E30@DHRNASVXM06.danaher.org> References: <43D1B55674EC2244A707ACE779624362DB9E30@DHRNASVXM06.danaher.org> Message-ID: Yes, it is embedding, not encasing. That's why you use the longer processing schedule for larger tissues. Brains and spines typically require longer processing schedules than other tissues due to the higher fat levels because of the myelin. For whole mouse and rat brains, so long as they are properly fixed, process on average for about 1.5 hours per station. Works like a charm, no issues. Typical (trimmed in) brain sections can get by on about 45 minutes per station. I have even had to process whole large animal organs, of course this was under a hood and took several days to complete, but...... And, no, not all animal researchers perfuse. Quite often, the brain is removed prior to fixation. And depending on what is to be done with the slides after sectioning, frozens are not ideal. Morphometry may be altered, etc. It all depends on the desired end result. Sincerely, Catherine Simonson, HT (ASCP) The Jackson Laboratory Bar Harbor, ME On Mon, Apr 13, 2015 at 10:19 AM, Scouten, Charles < Charles.Scouten@leicabiosystems.com> wrote: > Are you really embedding, or just encasing? I have heard that paraffin > cannot penetrate that deeply, that small sections are necessary for the > paraffin to infiltrate the entire piece. Is this not true? > > Why not use frozen sections? You can encase the brain in premade gelatin > molds see this link: > > http://www.leicabiosystems.com/research/neuroscience/tissue-sectioning/details/product/leica-brain-blocker-one/ > and section gelatin and all. Every brain in the same plane of section. > > Do you use fixation perfusion, or just extract the soft brain? Animal > researchers routinely use perfusion for the better tissue quality. > > Cordially, > > Charles W. Scouten, Ph.D. > Applications Specialist > Leica Biosystems > Charles.Scouten@Leicabiosystems.com > http://www.myneurolab.com > Ph. 630 964 0501 > Cell 314 724 5920 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Catherine Simonson > Sent: Friday, April 10, 2015 12:38 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: whole mouse brains > > Hey there! > > I embed whole mouse brains on a regular basis. First, process on a longer > schedule (about 1 to 1.5 hours per station, really. Otherwise they are > under processed and will not cut well and you will have problems getting > them to stay on the slides during staining). Use the deep molds. Keep in > mind that the tissue will shrink (about 20 - 30 %) during processing so > they WILL fit for coronal sections. If need be, trim some of the olfactory > bulbs off (you probably would be facing those off on the microtome anyhow). > > Hope this helps, > > Catherine Simonson, HT (ASCP) > The Jackson Laboratory > Bar Harbor, ME > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Please be advised that this email may contain confidential information. If > you are not the intended recipient, please notify us by email by replying > to the sender and delete this message. The sender disclaims that the > content of this email constitutes an offer to enter into, or the acceptance > of, any agreement; provided that the foregoing does not invalidate the > binding effect of any digital or other electronic reproduction of a manual > signature that is included in any attachment. > From ramakrishna.samala <@t> ttuhsc.edu Mon Apr 13 09:40:19 2015 From: ramakrishna.samala <@t> ttuhsc.edu (Samala, Ramakrishna) Date: Mon Apr 13 09:40:30 2015 Subject: [Histonet] Re: Paraffin embedding of whole mouse brain In-Reply-To: <43D1B55674EC2244A707ACE779624362DB9EC9@DHRNASVXM06.danaher.org> Message-ID: Hello Histonet! Thank you all for your time and wonderful suggestions. This is the very first time, I have to work with paraffin embedded brain tissues. Based on the suggestions from Histonet colleagues, I have decided to obtain 200 um thick brain sections for processing and embedding. Since I have about 30 mouse brains, I have to be very careful in labeling all cassettes. So problem solved!!!!! Again thank you all Have a very good week Sincerely Ramakrishna Samala, Ph.D. Senior Research Associate Amarillo Research Building, School of Pharmacy, Texas Tech University Health Sciences Center 1406 S. Coulter Amarillo, TX 79106 The outcome of any serious research can only be to make two questions grow where only one grew before ?Thorstein Veblen On 4/13/15, 9:30 AM, "Scouten, Charles" wrote: >Are you really embedding, or just encasing? I have heard that paraffin >cannot penetrate that deeply, that small sections are necessary for the >paraffin to infiltrate the entire piece. Is this not true? > >Why not use frozen sections? You can encase the brain in premade gelatin >molds see this link: >http://cp.mcafee.com/d/FZsS92gscyhJ5xdUQsILIfFCXCQQm4n73hOqenTzqqb2bwWZPhO >Orjhohssd79EVvd79J6ZT3hPtdBAsrzGKBoOEjxLkRnjZ1kVJAWmXQ6PsVJAWmXQ6PrOba11dZ >_HYyOCYUzvHTbFIFIYC---UCZORQX8FGEEKsG7DR8OJMddECSjt-hojuv78I9CzATsS02f-lJ8 >iHgJivGQVv8_jZzoDAfrzmBFv2TVelad-nFZFOH2k2e8vfp7QNqdk6XjM_VmQKgzIE5O1Zr5vy >nH3YdS9X4_WtiRmV_057_aSA9lEmFfRqsKrsohudwIqid40mSeKKOwq8fl8uwxa14Qgltd41wD >k_Ph0HlKDCy0x7pgdIcCYtznMVY5iQrl >and section gelatin and all. Every brain in the same plane of section. > >Do you use fixation perfusion, or just extract the soft brain? Animal >researchers routinely use perfusion for the better tissue quality. > > >Cordially, > >Charles W. Scouten, Ph.D. >Applications Specialist >Leica Biosystems >Charles.Scouten@Leicabiosystems.com >http://cp.mcafee.com/d/avndz9J5xdUQsILIfFCXCQQm4n73hOqenTzqqb2bwWZPhOOrjho >hssd79EVvd79J6ZT3hPtdBAsrzGKBoOEjxLkRnjZ1kVJAWmXQ6PsVJAWmXQ6PrOba11dZ_HYyO >CYUzvHTbFIFIYC---UCZORQX8FGEEKsG7DR8OJMddFCSjt-hojuv78I9CzATsS02lbgZKdjZ8s >KrIjS9_QWBGJP-0af-lJ8iHgJivGQVsSUMyYr1oQAq80JItttB0QguGgZ12k29EwGWq831eF_C >y1mHtfd412eOwropdIgvrKJ9Lwge >Ph. 630 964 0501 >Cell 314 724 5920 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Samala, >Ramakrishna >Sent: Friday, April 10, 2015 9:00 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Paraffin embedding of whole mouse brain > >Hello Histonet! > >I would like to paraffin embed whole mouse brain to obtain coronal >sections. I spoke with customer support teams of several companies, I >could able to get 10 mm deep molds, but the mouse brain is about 13 mm >height. So, any help in this regard is much appreciated. > >Sincerely > >Ramakrishna Samala, Ph.D. >Senior Research Associate >Amarillo Research Building, School of Pharmacy, Texas Tech University >Health Sciences Center >1406 S. Coulter >Amarillo, TX 79106 > >The outcome of any serious research can only be to make two questions >grow where only one grew before -Thorstein Veblen > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://cp.mcafee.com/d/5fHCN0SyMCYqemnS7QPtPqqb2bzxEVd7bXNJd5x5MtuVEVpdFEI >8Ke6zAQsLCzASzuXxEVKCOOedNRniIpk9MTGqHF-wGsSOtbtW3pKsSOtbtW3pJV5B0wC-_R-hp >jushLRXBQSkSujvvvsjuVqWtAkRkknel3PWApmU6CSjr9K_8I9LfzAm4PhOrKr01DOFeDdPFlK >pcNZ9_67uwTwCHIcfBisEeRMDaAWwQToDIj_FRblrDY0kvYHqgBmxqA_lFOVJNx5US2NF8Qg1r >oWWXa1EwZkxW24E4jh1lQQg62tj_d42JmWuq824tB0SMOrAM56 >Please be advised that this email may contain confidential information. >If you are not the intended recipient, please notify us by email by >replying to the sender and delete this message. The sender disclaims that >the content of this email constitutes an offer to enter into, or the >acceptance of, any agreement; provided that the foregoing does not >invalidate the binding effect of any digital or other electronic >reproduction of a manual signature that is included in any attachment. From mucram11 <@t> comcast.net Mon Apr 13 10:39:33 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon Apr 13 10:39:49 2015 Subject: [Histonet] Thank You For All Your Help Message-ID: <415030367.975627.1428939573563.JavaMail.zimbra@comcast.net> Last week I asked for suggestion to help out a young man at a University where he had few resources to help him with a bone project.? I had given him several suggestions and wanted to be sure he got as much information as possible to help him complete the project.? Since it was not at a medical school and he had only one connection for help who suggested he contact me we ALL go thim through.? He is extremely gratful to everyone. ? Thank You from Kiernan, ? Pam From jpiche <@t> wtbyhosp.org Mon Apr 13 12:06:32 2015 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Mon Apr 13 12:06:38 2015 Subject: [Histonet] p16 In-Reply-To: <54F720CF020000810000B31E@mail.bellin.org> References: <631955447A364B45B9458D2905635110D7C18188@WIN08-MBX-01.wtbyhosp.org> <54F720CF020000810000B31E@mail.bellin.org> Message-ID: <631955447A364B45B9458D2905635110D8BC33F1@WIN08-MBX-01.wtbyhosp.org> Thanks! From: Shannon Kleiner [mailto:shklei@bellin.org] Sent: Wednesday, March 04, 2015 4:12 PM To: histonet@lists.utsouthwestern.edu; Piche, Jessica Subject: Re: [Histonet] p16 Hello Jessica, We use Cintec p16 on our Bond Max. Here is a link you may find useful.... http://www.ventana.com/cervical Shannon Logan HTL (ASCP) Green Bay, WI >>> "Piche, Jessica" > 3/4/2015 1:38 PM >>> Hi Everyone, I was wondering if people are using the p16 antibody and if so where are you getting it from? Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From jshelley <@t> sanfordburnham.org Mon Apr 13 12:17:17 2015 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Mon Apr 13 12:17:22 2015 Subject: [Histonet] FSH Spring May Meeting Message-ID: Hello Florida Histonetters and Beyond!!! I wanted to let you know that we only have a week and half left for guaranteed room rate and availability for the hotel where our meeting is being held. Please act quickly if you are considering attending our meeting. I am including the content of a previously sent email. Please read further. I wanted to let everyone know that the FSH 2015 Meeting Program is complete and we are looking forward to a great meeting on May 14-17, 2015 at the Lake Buena Vista Palace Hotel and Spa. We especially invite those here in the State of Florida to come in order to fulfill your CEU requirements for both your state licensure and for you HT/HTL certification. We are also extending the invitation to those of you outside the state if you are looking to get away from the winter and would like the wonderful bright sun and vacation atmosphere of our location. Our meeting will be nestled within the Disney area and would be a great opportunity for learning and relaxation. I have included some key links to be able to make your plans that much easier. I look forward to seeing you at the meeting. Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402&hotelID=6579 Need to book no later than 4-23-15 Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155&lbrd=1&rtypeid=380141 Sincerely, John Shelley 2014-16 FSH President From b-frederick <@t> northwestern.edu Mon Apr 13 14:01:29 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Apr 13 14:01:38 2015 Subject: [Histonet] Schiffs Message-ID: Having a brain fart all- does the Schiff go into the formaldehyde or vice versa to test if the Schiffs is still good? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From JMacDonald <@t> mtsac.edu Mon Apr 13 14:45:23 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Apr 13 14:45:31 2015 Subject: [Histonet] Schiffs In-Reply-To: References: Message-ID: Schiff into 10 mL of 37-40% formaldehyde From: Bernice Frederick To: "Histonet@lists.utsouthwestern.edu" Date: 04/13/2015 12:40 PM Subject: [Histonet] Schiffs Sent by: histonet-bounces@lists.utsouthwestern.edu Having a brain fart all- does the Schiff go into the formaldehyde or vice versa to test if the Schiffs is still good? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Apr 13 17:51:02 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Apr 13 21:51:49 2015 Subject: [Histonet] Reference needed Message-ID: Does anyone have a reference for the theory of: 1. The use of soapy water to prevent fatty tissue from blowing apart in the flotation bath? 2. The use of ammonia water to rehydrate tissues? Many of us use these tricks, but is there a source for the theory? Thanks, Jennifer MacDonald From jkiernan <@t> uwo.ca Tue Apr 14 00:22:46 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Apr 14 00:22:50 2015 Subject: [Histonet] Reference needed In-Reply-To: <7330ed60a635.552ca40b@uwo.ca> References: <72e0f727edb0.552ca21e@uwo.ca> <72d08e84a135.552ca25a@uwo.ca> <72d0f9f8c4f8.552ca298@uwo.ca> <72d0e95ec2b3.552ca2d6@uwo.ca> <73e0f36fe27b.552ca314@uwo.ca> <73309393f519.552ca352@uwo.ca> <7330eae7e441.552ca3cd@uwo.ca> <7330ed60a635.552ca40b@uwo.ca> Message-ID: <73309e4e9d10.552c5dd6@uwo.ca> Do you need a source to satisfy ignorant officials? Try a good textbook that shows up in second-hand bookshops. Brown,GC 1978 An Introduction to Histotechnology. New York: Appleton-Century-Crofts. It doesn't seem to have an ISBN. John Kiernan = = = On 13/04/15, Jennifer MacDonald wrote: > Does anyone have a reference for the theory of: > 1. The use of soapy water to prevent fatty tissue from blowing > apart in the flotation bath? > 2. The use of ammonia water to rehydrate tissues? > Many of us use these tricks, but is there a source for the theory? > Thanks, > Jennifer MacDonald > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From JMacDonald <@t> mtsac.edu Tue Apr 14 01:33:09 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Apr 14 01:33:16 2015 Subject: [Histonet] Reference needed In-Reply-To: <73309e4e9d10.552c5dd6@uwo.ca> References: <72e0f727edb0.552ca21e@uwo.ca> <72d08e84a135.552ca25a@uwo.ca> <72d0f9f8c4f8.552ca298@uwo.ca> <72d0e95ec2b3.552ca2d6@uwo.ca> <73e0f36fe27b.552ca314@uwo.ca> <73309393f519.552ca352@uwo.ca> <7330eae7e441.552ca3cd@uwo.ca> <7330ed60a635.552ca40b@uwo.ca> <73309e4e9d10.552c5dd6@uwo.ca> Message-ID: No. Someone in another lab asked me if I knew if there was documentation of how these work. I did not have this in my resources. I will look for the book you mentioned. It is more curiosity than need. I am also curious of the chemistry. Thank you. Jennifer From: John Kiernan To: Jennifer MacDonald , histonet@lists.utsouthwestern.edu Date: 04/13/2015 10:23 PM Subject: Re: [Histonet] Reference needed Do you need a source to satisfy ignorant officials? Try a good textbook that shows up in second-hand bookshops. Brown,GC 1978 An Introduction to Histotechnology. New York: Appleton-Century-Crofts. It doesn't seem to have an ISBN. John Kiernan = = = On 13/04/15, Jennifer MacDonald wrote: Does anyone have a reference for the theory of: 1. The use of soapy water to prevent fatty tissue from blowing apart in the flotation bath? 2. The use of ammonia water to rehydrate tissues? Many of us use these tricks, but is there a source for the theory? Thanks, Jennifer MacDonald _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Yves.Heremans <@t> vub.ac.be Tue Apr 14 02:26:00 2015 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Tue Apr 14 02:27:00 2015 Subject: [Histonet] transcardial fixation Message-ID: <51D52790-789D-4189-92B3-630B6AAD93AE@vub.ac.be> Dear Histonetters, We routinely perfuse mice with fixative before taking out organs. Perfusion with fixative (4% PFA or 10% NBF) is done for only a few minutes (max. 2 min.). During that short timespan, the entire mouse becomes stiff. Can this stifness be taken as a sign of good initial fixation (we post-fix the organs overnight at room temp) or is this stifness not entirely related to fixation ? Yves From melissa <@t> alliedsearchpartners.com Tue Apr 14 10:22:46 2015 From: melissa <@t> alliedsearchpartners.com (Melissa Owens) Date: Tue Apr 14 10:22:57 2015 Subject: [Histonet] Veterinary Pathologist Needed in AZ for Part Time Work Message-ID: Hello Histonet, I am reaching out because I am in search of a Veterinary Pathologist willing to sign off on about 10 cases per week (and growing) in Scottsdale, AZ area. Payment is negotiable. Please contact me for a little more information and to send me your resume if interested. I would need to secure your resume prior to disclosing details of the location and name of facility. Thank you, Melissa Owens Allied Search Partners 888-388-7571 x. 102 Melissa@alliedsearchpartners.com From cynthia.hunter <@t> crystaldatalist.com Thu Apr 9 09:21:48 2015 From: cynthia.hunter <@t> crystaldatalist.com (Cynthia Hunter) Date: Tue Apr 14 15:40:33 2015 Subject: [Histonet] RE: OB/GYN Database Message-ID: Hi Good day to you, I am writing this email to check if you have got the below email and have any update on the same. Thanks and I will look forward to your response Best Regards Cynthia From: Cynthia Hunter [mailto:cynthia.hunter@crystaldatalist.com] Sent: Monday, March 30, 2015 11:06 AM To: 'histonet@lists.utsouthwestern.edu' Subject: OB/GYN Database Hi, I want to check if you're interested in OB/GYN Database? Specialties- Pregnancy Care, Family Practice, Internal Med, Family Med, Psychiatry, Independent Practices, Hospital Owned and many more.. The lists comes with complete details such as Name, Company, Website, Title, Phone number, Fax number, Email address, Country, SIC code, zip code, revenue & employees. Our Capabilities: * Email campaigns with dedicated Login credential to check the reports on real time Basis *Appending Services: We can append any missing information on your existing database and append additional information too making sure all the white Space is filled *Digital Marketing: We execute Social Media campaigns. And assist on SEO, Website Design & Development and Content Marketing Let me know a convenient time to schedule a call and help us give you more insights. Look forward speaking with you and assuring our list will fetch better results. Best regards Cynthia Hunter Online Marketing Executive If you're not interested to receive further emails, please reply with the subject line as "Leave out" From emily.jacob <@t> crystaldatalist.com Thu Apr 9 12:00:20 2015 From: emily.jacob <@t> crystaldatalist.com (Emily Jacob) Date: Tue Apr 14 15:40:35 2015 Subject: [Histonet] RE: Healthcare Specialist Message-ID: Hi Good day to you, I am writing this email to check if you have got the below email and have any update on the same. Thanks and I will look forward to your response Best Regards Emily From: Emily Jacob [mailto:emily.jacob@crystaldatalist.com] Sent: Monday, March 30, 2015 12:02 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Healthcare Specialist Hi, I hope you are the right person to discuss about Healthcare Specialist Database - 2013 which comprise complete contact details (verified Phone Number, Fax Number, Verified Email Address, Employee Size, Revenue size, SIC Code, Industry Type and many more). Few of the lists mentioned: Anesthesiology Doctors , Cardiology, Chiropractic Doctors, Dermatology Doctors, Emergency Medicine Doctors, Family Practice, Gastroenterology, Gynecology Doctors , Hematology , Internal medicine Doctors, Neurology Doctors, Obstetrics/Gynecology Doctors , Oncology Doctors, Ophthalmology Doctors, Optometry Doctors, Orthopedic Surgery, Otorhinolaryngology Doctors, Pathology Doctors, Pediatrics Doctors, Plastic Surgery, Psychiatry, Radiology, Urology Doctors, Nurse Mailing, Registered Nurses and many more. Please let me know your target audience and geographical area, so that we can send you more information about our services. Looking forward to hear from you. Regards, Emily Jacob | List acquisition | Technology Lists | Email/Data Appending | Search Engine Optimization | If you do not wish to receive future emails from us, please reply as 'leave out' From jo-ann.bader <@t> mcgill.ca Wed Apr 15 10:39:49 2015 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Wed Apr 15 10:39:54 2015 Subject: [Histonet] ORO tissue falling off Message-ID: <8C36045F0065CE48906E684F15FD4CB6321C3F75@EXMBX2010-6.campus.MCGILL.CA> We are having difficulty with a particulate set of very, very fatty mouse livers. The normal livers from this set stay on the slides the fatty livers fall off. We have used different types of charged slides and we have even tried to drench the charged slides in Stay-On, dry them and then put the frozen tissues on (despirate times call for despirate measures). No luck Does anyone have any other ideas. Help Help Jo-Ann Bader Histology Coordinator Goodman Cancer Research Center 1600 Pine Ave. W, Room 312 Montreal Quebec, H3A 1A3 Email: jo-ann.bader@mcgill.ca Office Tel: 514-398-5647 Lab: Tel: 514-398-8270 From Allison.Scott <@t> harrishealth.org Wed Apr 15 10:43:11 2015 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Wed Apr 15 10:43:30 2015 Subject: [Histonet] PA Competency Message-ID: Hello to all in histoland. Does any one have a competency evaluation for a Pathologist Assistant that they would be willing to share. Any help in this would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From llewllew <@t> shaw.ca Wed Apr 15 10:57:15 2015 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Apr 15 10:57:25 2015 Subject: [Histonet] ORO tissue falling off In-Reply-To: <8C36045F0065CE48906E684F15FD4CB6321C3F75@EXMBX2010-6.campus.MCGILL.CA> References: <8C36045F0065CE48906E684F15FD4CB6321C3F75@EXMBX2010-6.campus.MCGILL.CA> Message-ID: <552E8A5B.7000804@shaw.ca> There are a couple of old techniques you might try. 1. Smear some Mayer's egg albumen on a slide as you would normally do, i.e. very thinly, then hold it in a flame until it smokes. Allow to cool and pick up the section. Allow to drain well. 2. Same as 1, but place the slide with the picked up tissue in a coplin jar with a couple of mL concentrated formalin in the bottom for a hour or so to fix the albumen. 3. Same as 1 and 2, but use 1% gelatin instead of Mayer's egg albumen. 4. If all else fails, stain free floating sections of about 15 microns and pick up on a slide immediately before coverslipping. This was the way fat stains were done years ago before the cryostat. Bryan Llewellyn Jo-Ann Bader, Ms. wrote: > We are having difficulty with a particulate set of very, very fatty mouse livers. The normal livers from this set stay on the slides the fatty livers fall off. We have used different types of charged slides and we have even tried to drench the charged slides in Stay-On, dry them and then put the frozen tissues on (despirate times call for despirate measures). No luck Does anyone have any other ideas. Help Help > > Jo-Ann Bader > Histology Coordinator > Goodman Cancer Research Center > 1600 Pine Ave. W, > Room 312 > Montreal Quebec, H3A 1A3 > Email: jo-ann.bader@mcgill.ca > Office Tel: 514-398-5647 > Lab: Tel: 514-398-8270 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Wed Apr 15 11:11:26 2015 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Apr 15 11:11:35 2015 Subject: [Histonet] RELIA Histology Careers Bulletin 4-15-2015 What's Right in Your Backyard? Message-ID: <00c701d07796$d38fa2b0$7aaee810$@earthlink.net> Hi Histonetters! So how is your week going? I cut out of work early yesterday and headed over to the beach to watch the launch of the SpaceX Falcon 9 Rocket. It was amazing!!!! I've been promising myself for years that I would go over and watch a launch at the beach. Living in Orlando I rarely miss a launch because I can walk out in my front yard to see them but it's been years since I have been over to the coast for a launch. It got me thinking. I remember the Apollo launches on the beach when I was a kid! I didn't realize until I got to college that the rocket launches weren't as big a deal everyplace else. (You guys don't have NASA channel)? They don't show launches on the evening news? The Space Program - That's one of the things that are special to me about living in Florida. I want to ask you. What is special to you about where you live now? What is special to you about where you are from? What is special to you about where you want to live? I'm especially curious because I love to travel and since I recruit nationwide it's always interesting to hear what is unique and special in every area. So if you have a second please drop me a line. I would love to hear from you. I have some exciting job opportunities to share with you as well. All of these are full time permanent positions. My clients offer excellent compensation benefits and in most cases relocation and or sign on bonuses. These Clients are ready to interview and hire right away!!! LEADERSHIP/SUPERVISORY: AP Manager - Chicago Area Histology Supervisor - Flagstaff, AZ IHC Specialist - Shenandoah Valley, VA Lead Histotechnologist - St. Louis, MO TECHNICIANS/TECHNOLOGISTS: Dermpath Histotech - Birmingham, AL exciting new client! Histotechnician - Norfolk, VA - 15000 Sign on Bonus !!! Dermpath Histotech - Tyler, TX Learn Mohs!! Histotechnologist - Charlotte, NC great benefits and stability! Histotechnician - Hammond, IN (near Chicago) ASCP or elig.! Histotechnician - Kingsport, TN - Private Lab dayshift Dermpath Histo - Kansas City, MO CLIA Qual/Gross-learn Mohs Histotechnician - Nashville, TN nice benefits, evening shift Lead Histotech - St. Louis, MO beautiful lab, and great team! If you or anyone you know is interested in hearing more about any of these opportunities please contact me. I can be reached at relia1@earthlink.net toll free at 866-607-3542 or on my cell call/text 407-353-5070. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From ruio7 <@t> hotmail.com Wed Apr 15 14:16:04 2015 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Wed Apr 15 14:16:08 2015 Subject: [Histonet] soften beak In-Reply-To: References: Message-ID: Thank you for suggestions for softening beak. Yes, I actually processed the whole head for decalcifying first and then place the sample in 10% KOH for 30 mins. It was my first attempt to process beak (including a whole head) thus, i did not know i should have processed softening beak first. Anyway, after placing the beak in 10% KOH for 30 mins, it did not seem to soften enough. However, I did not overly place it in solution because it may destroy the microscopic structure. How soft the nail becomes after 10 % KOH or Nair treatment, such as you can bent the nail easily etc..? Any suggestion would be appreciated. rui *** From: ruio7@hotmail.com To: histonet@lists.utsouthwestern.edu Date: Fri, 10 Apr 2015 05:23:01 +0900 Subject: [Histonet] soften beak Hi, I am just wondering what you would recommend to soften avian beak for paraffin processing. I found a protocol that KOH could be used to soften keratin in nail, however, I have not been able to find that this method is applicable specific to beak. Another concern is if i use KOH for an entire head, does KOH affect (e.g. damage) soft tissues? Thank you in advance, rui _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dw18 <@t> uchicago.edu Wed Apr 15 16:06:05 2015 From: dw18 <@t> uchicago.edu (David Wright) Date: Wed Apr 15 16:06:09 2015 Subject: [Histonet] RE: perfusion stiffness Message-ID: Hi Yves & Histonet It is certainly a good sign if limbs etc are stiff after perfusion, but maybe not a guarantee that the target organ is perfect given the short perfusions you describe. Definitely, if I don't see stiffness I worry, check for a torn aortic arch (you are doing it transcardially, I presume), adjust the needle placement and run more fixative until everything is stiff. Do you harvest organs from all over the body? You can improve the efficiency of perfusion by limiting it to the regions of interest. For example, I perfuse rats for brain extraction and clamp off the descending vessels (clamped to the spine) at the level of the diaphragm. The lower half of the body then doesn't get rigid, but the upper half does so more fully/faster. (In rats, I perfuse for much longer than you describe and post-fix too.) Note there's two kinds of stiffness - an immediate, zombie-like outstretching of the forelimbs (& tail wiggling if you do the whole body) which is immediately satisfying as a sign of good needle placement but only happens with a very fresh cadaver, and a more generalized, slower rigidity. For my brains, I check for neck muscle rigidity as well as the forelimb zombie effect. best wishes - David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 ________________________________________ Histonet Digest, Vol 137, Issue 17 Message: 8 Date: Tue, 14 Apr 2015 09:26:00 +0200 From: Yves Heremans Subject: [Histonet] transcardial fixation To: histonet@lists.utsouthwestern.edu Message-ID: <51D52790-789D-4189-92B3-630B6AAD93AE@vub.ac.be> Content-Type: text/plain; charset=us-ascii Dear Histonetters, We routinely perfuse mice with fixative before taking out organs. Perfusion with fixative (4% PFA or 10% NBF) is done for only a few minutes (max. 2 min.). During that short timespan, the entire mouse becomes stiff. Can this stifness be taken as a sign of good initial fixation (we post-fix the organs overnight at room temp) or is this stifness not entirely related to fixation ? Yves End of ***************************************** From tgenade <@t> gmail.com Wed Apr 15 16:10:23 2015 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Wed Apr 15 16:10:27 2015 Subject: [Histonet] Bouin's, formic acid & and sirius red staining Message-ID: Hello, Some questions regarding Bouin's solution. I was told, back when I was doing my PhD and new very little, that I should fix my fish in Bouin's as it will decalsify the bones. Well, Bouin's fixed fish were easier to cut than PFA fixed fish... but I read today that by adding formic acid the decalsification is better. (I must confess, that after Buoin's fixation I still had to soak the tissue face in some dilute nitric acid now and then...) Another reference said that the formaldehyde should be replaced with formic acid. So which is it: add formic acid or replace formaldehyde? And if the former, how much do you add? Second question: a colleague and I want to stain for collagen in diseased kidneys. The fixative of choice for soft tissue is Bouin's... But the staining protocol called for a solution of picric acid and sirius red. Is the picric acid needed if I haven't washed the picric acid from the Bouin's fixation out of the tissue? I was told once that the picric acid was for contrast... Is this BS? Does the picric acid play an important chemical role in the staining? I would like to avoid the need for a bottle of saturated picric acid on the lab shelf here in Iowa where the winter low humidity desiccates everything... I'm hoping this protocol, http://www.ihcworld.com/_protocols/special_stains/sirius_red.htm , can be modified to omit the picric acid. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From JWatson <@t> gnf.org Wed Apr 15 16:38:21 2015 From: JWatson <@t> gnf.org (James Watson) Date: Wed Apr 15 16:38:26 2015 Subject: [Histonet] RE: perfusion stiffness In-Reply-To: References: Message-ID: Watch the liver, it will change color as the blood is washed out. Are you perfusing first with PBS to rinse the blood out, if you start with formalin small blood vessels and capillaries can become blocked by the blood that has coagulated when exposed to the formalin. We actually flush with 10% sucrose first since brains flushed with PBS can have up to 20% shrinkage. I have not heard of a good theory behind body stiffness after perfusion yet, pretty sure it is not the effect of actual cross linking since that takes time. Is someone knows the cause please let me know. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Wright Sent: Wednesday, April 15, 2015 2:06 PM To: histonet@lists.utsouthwestern.edu Cc: Yves Heremans Subject: [Histonet] RE: perfusion stiffness Hi Yves & Histonet It is certainly a good sign if limbs etc are stiff after perfusion, but maybe not a guarantee that the target organ is perfect given the short perfusions you describe. Definitely, if I don't see stiffness I worry, check for a torn aortic arch (you are doing it transcardially, I presume), adjust the needle placement and run more fixative until everything is stiff. Do you harvest organs from all over the body? You can improve the efficiency of perfusion by limiting it to the regions of interest. For example, I perfuse rats for brain extraction and clamp off the descending vessels (clamped to the spine) at the level of the diaphragm. The lower half of the body then doesn't get rigid, but the upper half does so more fully/faster. (In rats, I perfuse for much longer than you describe and post-fix too.) Note there's two kinds of stiffness - an immediate, zombie-like outstretching of the forelimbs (& tail wiggling if you do the whole body) which is immediately satisfying as a sign of good needle placement but only happens with a very fresh cadaver, and a more generalized, slower rigidity. For my brains, I check for neck muscle rigidity as well as the forelimb zombie effect. best wishes - David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 ________________________________________ Histonet Digest, Vol 137, Issue 17 Message: 8 Date: Tue, 14 Apr 2015 09:26:00 +0200 From: Yves Heremans Subject: [Histonet] transcardial fixation To: histonet@lists.utsouthwestern.edu Message-ID: <51D52790-789D-4189-92B3-630B6AAD93AE@vub.ac.be> Content-Type: text/plain; charset=us-ascii Dear Histonetters, We routinely perfuse mice with fixative before taking out organs. Perfusion with fixative (4% PFA or 10% NBF) is done for only a few minutes (max. 2 min.). During that short timespan, the entire mouse becomes stiff. Can this stifness be taken as a sign of good initial fixation (we post-fix the organs overnight at room temp) or is this stifness not entirely related to fixation ? Yves End of ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfields <@t> mlkch.org Wed Apr 15 16:51:15 2015 From: cfields <@t> mlkch.org (Carol Fields) Date: Wed Apr 15 16:51:30 2015 Subject: [Histonet] FW: Cost of a Slide in LA Message-ID: <22e37e1ec5b54faba6e4b01626897811@mlk01exc02.mlkch.org> From: Carol Fields Sent: Wednesday, April 15, 2015 2:48 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Cost of a Slide in LA Hi Netters, Could someone tell me what the cost of a slide would be in the Los Angeles area? Also what labs charge in this area to make a slide? I realize I can figure this as I have many times but my boss was asking for a quick figure and I hope someone in this area might have a quick answer. Many thanks in advance!! Carole Fields MLKCH Los Angeles, CA From cfields <@t> mlkch.org Wed Apr 15 16:53:43 2015 From: cfields <@t> mlkch.org (Carol Fields) Date: Wed Apr 15 16:53:57 2015 Subject: [Histonet] FW: Cost of a Slide in LA Message-ID: <23e12e8aabef4b2384202c360862d5a5@mlk01exc02.mlkch.org> From: Carol Fields Sent: Wednesday, April 15, 2015 2:48 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Cost of a Slide in LA Hi Netters, Could someone tell me what the cost of a slide would be in the Los Angeles area? Also what labs charge in this area to make a slide? I realize I can figure this as I have many times but my boss was asking for a quick figure and I hope someone in this area might have a quick answer. Many thanks in advance!! Carole Fields MLKCH Los Angeles, CA From linda.prasad <@t> health.nsw.gov.au Wed Apr 15 19:07:10 2015 From: linda.prasad <@t> health.nsw.gov.au (Linda Prasad (SCHN)) Date: Wed Apr 15 19:07:31 2015 Subject: [Histonet] RE: ORO tissue falling off In-Reply-To: <8C36045F0065CE48906E684F15FD4CB6321C3F75@EXMBX2010-6.campus.MCGILL.CA> References: <8C36045F0065CE48906E684F15FD4CB6321C3F75@EXMBX2010-6.campus.MCGILL.CA> Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E8B9DF@xmdb03.nch.kids> Usually with the fatty tissues, I pick them up on superfrost slides and let it air dry for 2-3 days at room temperature and then perform the ORO stains. So far they seem to stay on. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Thursday, 16 April 2015 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ORO tissue falling off We are having difficulty with a particulate set of very, very fatty mouse livers. The normal livers from this set stay on the slides the fatty livers fall off. We have used different types of charged slides and we have even tried to drench the charged slides in Stay-On, dry them and then put the frozen tissues on (despirate times call for despirate measures). No luck Does anyone have any other ideas. Help Help Jo-Ann Bader Histology Coordinator Goodman Cancer Research Center 1600 Pine Ave. W, Room 312 Montreal Quebec, H3A 1A3 Email: jo-ann.bader@mcgill.ca Office Tel: 514-398-5647 Lab: Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From linda.prasad <@t> health.nsw.gov.au Wed Apr 15 19:29:33 2015 From: linda.prasad <@t> health.nsw.gov.au (Linda Prasad (SCHN)) Date: Wed Apr 15 19:29:50 2015 Subject: [Histonet] Bouin's, formic acid & and sirius red staining In-Reply-To: References: Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E8BA42@xmdb03.nch.kids> Hi Tryrone A Mass Trichrome stain will stain up the collagen in the diseased kidney. If the tissue has already been fixed in Bouin's Solution you can omit the picric acid step. Another good stain is the Curtis's Van Gieson Stain. Ive sent you the protocol for botht he stains. Hope it helps. Masson Trichrome Principal This method depends on the special action of phosphomolybdic acid when combined with red aniline dye. The acid lifts the colour first from the collagen and only later from the cytoplasm. The differentiation is stopped at the point where the Collagen only is colourless, and then another aniline dye (light green) is added to stain the collagen Reagents: 1. Ponceau Acid Fuchsin Warning: Suspected Carcinogen ? see MSDS Acid fuchsin (Sigma Aldrich CI 42685) 0.1g Ponceau de xylidene (CI 16150) 1g Distilled Water 99ml When dissolved, add 1ml Glacial Acetic Acid 2. Light Green Warning: Suspected Carcinogen ? see MSDS Light green (CI 42095) 2g Glacial Acetic acid 2ml Distilled water 98ml 3. Weigert's Iron Haematoxylin or Celestine Blue/Haematoxylin 4. 1% Phosphomolybdic Acid Phosphomolybdic acid 5g Distilled water 500ml Procedure: 1. Bring sections to distilled water. 2. Celestine blue 10 minute. 3. Wash well in water. 4. Harris' haematoxylin 10 minutes. 5. Differentiate and blue 6. Ponceau acid fuchsin solution 10min 7. Quick rinse in tap water 8. 1% Phosphomolybdic acid 5min 9. Do not rinse in water 10. Light green solution 2min 11. Rinse excess stain rapidly in water. 12. Dehydrate, clear & mount Results: Collagen, mucin green Muscle, Fibrin red Nuclei purple Curtis's Van Gieson Stain Principle: This solution is based on the same formula as Van Gieson with the exception of the use of Ponceau S rather than acid fuschin. Glacial Acetic Acid is used rather than hydrochloric acid to sharpen the staining results. Reagents: 1. 1% aqueous Ponceau S Ponceau S (CI 27195) 0.5g Distilled water 50ml 2. Curtis?s Van Gieson 1% aqueous Ponceau S 10ml Saturated aqueous picric acid 90ml Glacial Acetic Acid 1.5ml 3. Celestine Blue ? Haematoxylin procedure Procedure: 1. Dewax and hydrate sections. 2. Celestine blue 5 minute wash. 3. Wash well in water. 4. Harris' haematoxylin 5 minutes. 5. Wash in water and blue (do not differentiate). 6. Curtis Van Gieson stain 10 minutes (do not wash). 7. Rinse in absolute alcohol 8. Dehydrate, clear and mount. Results: Collagen Red Muscle, other tissues Yellow Nuclei Blue Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Thursday, 16 April 2015 7:10 AM To: histonet Subject: [Histonet] Bouin's, formic acid & and sirius red staining Hello, Some questions regarding Bouin's solution. I was told, back when I was doing my PhD and new very little, that I should fix my fish in Bouin's as it will decalsify the bones. Well, Bouin's fixed fish were easier to cut than PFA fixed fish... but I read today that by adding formic acid the decalsification is better. (I must confess, that after Buoin's fixation I still had to soak the tissue face in some dilute nitric acid now and then...) Another reference said that the formaldehyde should be replaced with formic acid. So which is it: add formic acid or replace formaldehyde? And if the former, how much do you add? Second question: a colleague and I want to stain for collagen in diseased kidneys. The fixative of choice for soft tissue is Bouin's... But the staining protocol called for a solution of picric acid and sirius red. Is the picric acid needed if I haven't washed the picric acid from the Bouin's fixation out of the tissue? I was told once that the picric acid was for contrast... Is this BS? Does the picric acid play an important chemical role in the staining? I would like to avoid the need for a bottle of saturated picric acid on the lab shelf here in Iowa where the winter low humidity desiccates everything... I'm hoping this protocol, http://www.ihcworld.com/_protocols/special_stains/sirius_red.htm , can be modified to omit the picric acid. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From mills <@t> 3scan.com Wed Apr 15 19:44:00 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Wed Apr 15 19:44:10 2015 Subject: [Histonet] RE: ORO tissue falling off In-Reply-To: <1217DDB3D7DE5E418E3D560A268EABD0E0E8B9DF@xmdb03.nch.kids> References: <8C36045F0065CE48906E684F15FD4CB6321C3F75@EXMBX2010-6.campus.MCGILL.CA> <1217DDB3D7DE5E418E3D560A268EABD0E0E8B9DF@xmdb03.nch.kids> Message-ID: +1 to Linda, but I have found no difference on overnight vs multiple days. Fatty liver is hard to do on all counts! It is tough enough sometimes to get a decent section on the slide. Thanks for the other suggestions, certainly something I would try in the future Yours Caroline Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Apr 15, 2015, at 5:07 PM, Linda Prasad (SCHN) wrote: > > Usually with the fatty tissues, I pick them up on superfrost slides and let it air dry for 2-3 days at room temperature and then perform the ORO stains. So far they seem to stay on. > > Linda Prasad | Senior Scientist | Histopathology > t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au > > Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia > Locked Bag 4001, Westmead 2145, NSW Australia > > ? Please consider the environment before printing this email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. > Sent: Thursday, 16 April 2015 1:40 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ORO tissue falling off > > We are having difficulty with a particulate set of very, very fatty mouse livers. The normal livers from this set stay on the slides the fatty livers fall off. We have used different types of charged slides and we have even tried to drench the charged slides in Stay-On, dry them and then put the frozen tissues on (despirate times call for despirate measures). No luck Does anyone have any other ideas. Help Help > > Jo-Ann Bader > Histology Coordinator > Goodman Cancer Research Center > 1600 Pine Ave. W, > Room 312 > Montreal Quebec, H3A 1A3 > Email: jo-ann.bader@mcgill.ca > Office Tel: 514-398-5647 > Lab: Tel: 514-398-8270 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From linda.prasad <@t> health.nsw.gov.au Wed Apr 15 19:47:21 2015 From: linda.prasad <@t> health.nsw.gov.au (Linda Prasad (SCHN)) Date: Wed Apr 15 19:47:39 2015 Subject: [Histonet] RE: ORO tissue falling off In-Reply-To: References: <8C36045F0065CE48906E684F15FD4CB6321C3F75@EXMBX2010-6.campus.MCGILL.CA> <1217DDB3D7DE5E418E3D560A268EABD0E0E8B9DF@xmdb03.nch.kids> Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E8BAE1@xmdb03.nch.kids> I Know fatty tissue is such a pain to cut. Bryan Llewellyn gave some really good techniques. Im going to try them out myself :) Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: Caroline Miller [mailto:mills@3scan.com] Sent: Thursday, 16 April 2015 10:44 AM To: Linda Prasad (SCHN) Cc: Jo-Ann Bader, Ms.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: ORO tissue falling off +1 to Linda, but I have found no difference on overnight vs multiple days. Fatty liver is hard to do on all counts! It is tough enough sometimes to get a decent section on the slide. Thanks for the other suggestions, certainly something I would try in the future Yours Caroline Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Apr 15, 2015, at 5:07 PM, Linda Prasad (SCHN) wrote: > > Usually with the fatty tissues, I pick them up on superfrost slides and let it air dry for 2-3 days at room temperature and then perform the ORO stains. So far they seem to stay on. > > Linda Prasad | Senior Scientist | Histopathology > t: (02) 9845 3306 | f: (02) 9845 3318 | e: > linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au > > Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia > Locked Bag 4001, Westmead 2145, NSW Australia > > ? Please consider the environment before printing this email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. > Sent: Thursday, 16 April 2015 1:40 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ORO tissue falling off > > We are having difficulty with a particulate set of very, very fatty > mouse livers. The normal livers from this set stay on the slides the > fatty livers fall off. We have used different types of charged slides > and we have even tried to drench the charged slides in Stay-On, dry > them and then put the frozen tissues on (despirate times call for > despirate measures). No luck Does anyone have any other ideas. Help > Help > > Jo-Ann Bader > Histology Coordinator > Goodman Cancer Research Center > 1600 Pine Ave. W, > Room 312 > Montreal Quebec, H3A 1A3 > Email: jo-ann.bader@mcgill.ca > Office Tel: 514-398-5647 > Lab: Tel: 514-398-8270 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > *********** This email and any files transmitted with it are > confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > *********** _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From stephen.jew <@t> sydney.edu.au Wed Apr 15 19:59:21 2015 From: stephen.jew <@t> sydney.edu.au (Stephen KumJew) Date: Wed Apr 15 19:59:53 2015 Subject: [Histonet] Astrocyte immunohistochemical marker Message-ID: I was wondering if anyone could recommend an antibody (and best dilution) which is more effective than GFAP in staining both resting and active astrocytes in human brain please. Thanks STEPHEN KUM JEW | Senior Technical Officer Discipline of Pathology | School of Medical Sciences THE UNIVERSITY OF SYDNEY Charles Perkins Centre Hub | Building D17 | Camperdown | NSW | 2050 | Australia. Delivery address: The CPC Hub Dock, Orphans School Creek Lane, Camperdown NSW 2050. T +61 2 9036 9027| F +61 2 8627 1606 E stephen.jew@sydney.edu.au | W http://sydney.edu.au/medicine/pathology/ This email plus any attachments to it are confidential. Any unauthorised use is strictly prohibited. If you receive this email in error, please delete it and any attachments. Please consider the environment before printing this email. From jkiernan <@t> uwo.ca Wed Apr 15 17:43:47 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Apr 15 20:11:00 2015 Subject: [Histonet] Bouin's, formic acid & and sirius red staining In-Reply-To: <7210b2a52685f.552ee972@uwo.ca> References: <71e0909c27290.552ee783@uwo.ca> <73c0c80320f22.552ee7bf@uwo.ca> <7250e11d273d3.552ee7fe@uwo.ca> <722099b023eef.552ee83c@uwo.ca> <7330b8cf22bb1.552ee87a@uwo.ca> <7330bcba27e01.552ee8b8@uwo.ca> <73c08e0325b55.552ee8f6@uwo.ca> <7370b01722a88.552ee934@uwo.ca> <7210b2a52685f.552ee972@uwo.ca> Message-ID: <73e080c320984.552ea353@uwo.ca> Fixation must be adequate before decalcifying. 12-24h in Bouin is OK. My favourite formic decalcifier is that of Clark (1954) Am. J. Clin. Path. 24: 1113-1116. It's a buffer (pH2.0) made by mixing 90% formic acid 250ml, water 750 ml and sodium formate (anhydrous) 34g. Keeps for ever. Check the reference. Never trust a recipe you read on the internet! The picric acid in picro-sirius red is a necessary component of the staining solution. See Junqueira et al 1979 Histochem. J. 11: 447-455. It's important to have the correct red dye; there is more than one "sirius red". Use C.I. 35780, Direct red 80. John Kiernan London, Canada = = = On 15/04/15, Tyrone Genade wrote: > Hello, > > Some questions regarding Bouin's solution. > > I was told, back when I was doing my PhD and new very little, that I should > fix my fish in Bouin's as it will decalsify the bones. Well, Bouin's fixed > fish were easier to cut than PFA fixed fish... but I read today that by > adding formic acid the decalsification is better. (I must confess, that > after Buoin's fixation I still had to soak the tissue face in some dilute > nitric acid now and then...) Another reference said that the formaldehyde > should be replaced with formic acid. So which is it: add formic acid or > replace formaldehyde? And if the former, how much do you add? > > Second question: a colleague and I want to stain for collagen in diseased > kidneys. The fixative of choice for soft tissue is Bouin's... But the > staining protocol called for a solution of picric acid and sirius red. Is > the picric acid needed if I haven't washed the picric acid from the Bouin's > fixation out of the tissue? I was told once that the picric acid was for > contrast... Is this BS? Does the picric acid play an important chemical > role in the staining? I would like to avoid the need for a bottle of > saturated picric acid on the lab shelf here in Iowa where the winter low > humidity desiccates everything... I'm hoping this protocol, > http://www.ihcworld.com/_protocols/special_stains/sirius_red.htm , can be > modified to omit the picric acid. > > Thanks > -- > Tyrone Genade > Orange City, Iowa > tel: (+1) 712 230 4101 > http://tgenade.freeshell.org > ******************************************************************************** > Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. > To find out how to receive this FREE gift visit http://www.alpha.org. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From algranth <@t> email.arizona.edu Wed Apr 15 23:29:40 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Apr 15 23:29:47 2015 Subject: [Histonet] RE: ORO tissue falling off In-Reply-To: <1217DDB3D7DE5E418E3D560A268EABD0E0E8B9DF@xmdb03.nch.kids> References: <8C36045F0065CE48906E684F15FD4CB6321C3F75@EXMBX2010-6.campus.MCGILL.CA>, <1217DDB3D7DE5E418E3D560A268EABD0E0E8B9DF@xmdb03.nch.kids> Message-ID: I used to be the Queen of ORO in my lab at the University of Arizona. I had some of the fattiest livers that could be possible from different research projects. I never had a problem with the livers staying on the slide. I used Epic coated slides or Stat Lab slides and the protocol was from Freida's second edition. The stain was from PolyScientific R&D. I cut the sections at 5 microns, let them sit in the cryostat for about 30 minutes and then fixed in 37% Formaldehyde for about 10-30 minutes - never really timed it. All steps of staining were done gently, one slide at a time, no matter how many slides I had to stain. Sections were beautiful - wish I could post a picture here. Retired now - sometimes I miss doing things like this. Andi Grantham ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Linda Prasad (SCHN) [linda.prasad@health.nsw.gov.au] Sent: Wednesday, April 15, 2015 5:07 PM To: 'Jo-Ann Bader, Ms.'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: ORO tissue falling off Usually with the fatty tissues, I pick them up on superfrost slides and let it air dry for 2-3 days at room temperature and then perform the ORO stains. So far they seem to stay on. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ? Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Thursday, 16 April 2015 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ORO tissue falling off We are having difficulty with a particulate set of very, very fatty mouse livers. The normal livers from this set stay on the slides the fatty livers fall off. We have used different types of charged slides and we have even tried to drench the charged slides in Stay-On, dry them and then put the frozen tissues on (despirate times call for despirate measures). No luck Does anyone have any other ideas. Help Help Jo-Ann Bader Histology Coordinator Goodman Cancer Research Center 1600 Pine Ave. W, Room 312 Montreal Quebec, H3A 1A3 Email: jo-ann.bader@mcgill.ca Office Tel: 514-398-5647 Lab: Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From jkiernan <@t> uwo.ca Thu Apr 16 00:45:17 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Apr 16 00:45:22 2015 Subject: [Histonet] Astrocyte immunohistochemical marker In-Reply-To: <7330fb3e140ff.552f4c54@uwo.ca> References: <73c0859e17c1d.552f4366@uwo.ca> <7330e11817323.552f43df@uwo.ca> <7320e50517dde.552f441d@uwo.ca> <73708a5f16b4a.552f445b@uwo.ca> <73e0fa86103ca.552f4499@uwo.ca> <7370c4b7144db.552f44d7@uwo.ca> <72509664103ef.552f4515@uwo.ca> <7250af3815d74.552f4553@uwo.ca> <72509f34116a4.552f4591@uwo.ca> <7250e5e414e39.552f45cf@uwo.ca> <73e0a91615602.552f460e@uwo.ca> <7350903e14214.552f464c@uwo.ca> <72b0cc2f1490c.552f468a@uwo.ca> <7390c96f14e35.552f46c8@uwo.ca> <7250ee621762d.552f4706@uwo.ca> <7250fb59166fd.552f4744@uwo.ca> <7370ce4b158a3.552f4783@uwo.ca> <7330bf6313829.552f47c1@uwo.ca> <73309dce140ff.552f47ff@uwo.ca> <7330880414222.552f483d@uwo.ca> <7330e5ee103a7.552f487c@uwo.ca> <7330da0915c62.552f48ba@uwo.ca> <73709c2a15239.552f49ac@uwo.ca> <72c0c4271244d.552f49ea@uwo.ca> <72c0d16c163b7.552f4a28@uwo.ca> <72c0c57c11cf4.552f4a66@uwo.ca> <7370d91f128e1.552f4aa4@uwo.ca> <7370ca6516af9.552f4ae3@uwo.ca> <7330a06517e22.552f4b5d@uwo.ca> <72b0919510917.552f4b9b@uwo.ca> <73c0950017161.552f4c15@uwo.ca> <7330fb3e140ff.552f4c54@uwo.ca> Message-ID: <7330e2ce15e16.552f061d@uwo.ca> Immunostaining for GFAP has been the definitive method for astrocyte cytoplasm for 30 years, and good primary antibodies have been around for at least 20 years. The distinction between different kinds of astrocyte is made by staining with serial dilutions of the primary antiserum. Reactive astrocytes have the highest concentrations of glial fibrillary acidic protein (GFAP) in their cytoplasms, and are immunostained by greatly diluted antisera. Normal fibrous astrocytes (in normal white matter and also with processes abutting ependyma, pia and small blood vessels) also contain plenty of GFAP, but are unlikely to be confused with astrocytes around a site of injury or in a tumour. Normal protoplasmic (or velate) astrocytes extend their processes into normal white and grey matter; their cytoplasm contains less GFAP than the cytoplasm of astrocyte in normal white matter. To detect reactive astrocytes you need to do serial dilutions of the primary antibody on sections of normal CNS. For detecting gliosis, use the dilution that fails to stain GFAP in grey matter. This should show astrogliosis strongly, and also the fibrous astrocytes normally present in white matter. A high concentration of anti-GFAP will stain everything in the CNS, because astrocyte processes are everywhere there. The traditional astrocyte stain is Cajal's gold-sublimate. This shows normal and gliotic fibrous astrocytes nicely, but it takes time. For protoplasmic astrocytes you need electron microscopy, wich takes even more time. John Kiernan Anatomy, UWO, London, Canada = = = On 15/04/15, Stephen KumJew wrote: > I was wondering if anyone could recommend an antibody (and best dilution) which is more effective than GFAP in staining both resting and active astrocytes in human brain please. > > Thanks > > STEPHEN KUM JEW | Senior Technical Officer > Discipline of Pathology | School of Medical Sciences > > THE UNIVERSITY OF SYDNEY > Charles Perkins Centre Hub | Building D17 | Camperdown | NSW | 2050 | Australia. > Delivery address: The CPC Hub Dock, Orphans School Creek Lane, Camperdown NSW 2050. > T +61 2 9036 9027| F +61 2 8627 1606 > E stephen.jew@sydney.edu.au | > W http://sydney.edu.au/medicine/pathology/ > > This email plus any attachments to it are confidential. Any unauthorised use is strictly prohibited. If you receive this email in error, please delete it and any attachments. > Please consider the environment before printing this email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jmoreira <@t> sidra.org Thu Apr 16 06:24:54 2015 From: jmoreira <@t> sidra.org (Joana Moreira) Date: Thu Apr 16 06:28:12 2015 Subject: [Histonet] IHC Billing Question Message-ID: Greetings from Doha! This was much probably discussed before, but I was wondering if you could help me with a query in regards to billing. For the sites that are still doing an IHC negative reagent control for each patient specimen, do you bill for the negative control? Using code 88341? I believe it should be billed (since when and if performed correctly the negative control follows a normal IHC technique) however I am completely new to the billing subject. My previous experience is based in Portugal and UK (where billing does not exist) and I've been introduced to this topic since I joined my current institution that will be following the North American Healthcare model. So... any help will be GREATLY appreciated!! Many Thanks in advance, Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From bburnett <@t> CapeCodHealth.org Thu Apr 16 08:47:46 2015 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Thu Apr 16 08:47:51 2015 Subject: [Histonet] RE: IHC Billing Question In-Reply-To: References: Message-ID: We recently added HER2 IHC testing in our lab which we are required to use a negative reagent control For each case. Is there a cpt code for negative reagent control reimbursement? Any information on this Would be much appreciated! Thanks Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology Expert physicians. Quality hospitals. Superior care. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joana Moreira Sent: Thursday, April 16, 2015 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Billing Question Greetings from Doha! This was much probably discussed before, but I was wondering if you could help me with a query in regards to billing. For the sites that are still doing an IHC negative reagent control for each patient specimen, do you bill for the negative control? Using code 88341? I believe it should be billed (since when and if performed correctly the negative control follows a normal IHC technique) however I am completely new to the billing subject. My previous experience is based in Portugal and UK (where billing does not exist) and I've been introduced to this topic since I joined my current institution that will be following the North American Healthcare model. So... any help will be GREATLY appreciated!! Many Thanks in advance, Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From g.papon <@t> argolight.com Thu Apr 16 08:59:47 2015 From: g.papon <@t> argolight.com (Gautier Papon) Date: Thu Apr 16 08:59:55 2015 Subject: [Histonet] Non photobleaching standard for fluorescence imaging systems. Message-ID: <8685B0D1-3DA5-4947-BC05-44EB2B2DA30F@argolight.com> Hello, Argolight is a young French company that offers solution to qualify the imaging quality of fluorescent systems using a combination of innovative ultra stable fluorescence standards and image processing algorithms. Our flagship product the Argo-M is the best product available on the market for QC fluorescents systems, it doesn?t photo bleach and last for 5 years. It also cost $5090. We are trying something new and are doing a ? kick-starter ? like campaign to produce a $1600 slide that would do most of what the Argo-M does, albeit a little less precise, if we can get 100 people interested. More info here : http://argolight.com/argo-check/ and here : www.argolight.com Thank you for your support. Best regards, Gautier Papon CEO - Argolight From mward <@t> wakehealth.edu Thu Apr 16 09:35:59 2015 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Apr 16 09:36:07 2015 Subject: [Histonet] RE: IHC Billing Question In-Reply-To: References: Message-ID: We have not been charging for the negative control, assuming that it was just a cost of doing business. I would be interested to hear if anyone has been charging for their negative controls as well. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, April 16, 2015 9:48 AM To: 'Joana Moreira'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC Billing Question We recently added HER2 IHC testing in our lab which we are required to use a negative reagent control For each case. Is there a cpt code for negative reagent control reimbursement? Any information on this Would be much appreciated! Thanks Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology Expert physicians. Quality hospitals. Superior care. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joana Moreira Sent: Thursday, April 16, 2015 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Billing Question Greetings from Doha! This was much probably discussed before, but I was wondering if you could help me with a query in regards to billing. For the sites that are still doing an IHC negative reagent control for each patient specimen, do you bill for the negative control? Using code 88341? I believe it should be billed (since when and if performed correctly the negative control follows a normal IHC technique) however I am completely new to the billing subject. My previous experience is based in Portugal and UK (where billing does not exist) and I've been introduced to this topic since I joined my current institution that will be following the North American Healthcare model. So... any help will be GREATLY appreciated!! Many Thanks in advance, Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Vorbeck <@t> baycare.org Thu Apr 16 10:37:28 2015 From: Melissa.Vorbeck <@t> baycare.org (Vorbeck, Melissa) Date: Thu Apr 16 10:37:33 2015 Subject: [Histonet] VIAS after market digital camera software Message-ID: Hey there, I was hoping someone out there has had success in using the now unsupported VIAS scope/digital camera with an after market software. Please, if you have, message me. Or, if you have not had success, information regarding what you have tried and have not been able to get to work would also be beneficial. Thank you :) Melissa Vorbeck Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From bszpunar <@t> umail.iu.edu Thu Apr 16 14:37:39 2015 From: bszpunar <@t> umail.iu.edu (Bryan Szpunar) Date: Thu Apr 16 14:37:42 2015 Subject: [Histonet] Re: IHC Billing Question Message-ID: I have never heard of anyone billing for a negative control and would highly recommend against doing so. As mentioned, it is a cost of doing business--which is why when CAP clarified that polymer detection systems did not require them, most cost-conscious labs quit running them. Regards, Bryan Szpunar, HT(ASCP) > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joana Moreira > Sent: Thursday, April 16, 2015 7:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC Billing Question > > Greetings from Doha! > > This was much probably discussed before, but I was wondering if you could > help me with a query in regards to billing. > For the sites that are still doing an IHC negative reagent control for > each patient specimen, do you bill for the negative control? Using code > 88341? > > I believe it should be billed (since when and if performed correctly the > negative control follows a normal IHC technique) however I am completely > new to the billing subject. My previous experience is based in Portugal and > UK (where billing does not exist) and I've been introduced to this topic > since I joined my current institution that will be following the North > American Healthcare model. So... any help will be GREATLY appreciated!! > > Many Thanks in advance, > Joana > > Joana Moreira > Supervisor - Anatomical Pathology > Department of Pathology > > Sidra Medical & Research Center > PO Box 26999 | Doha, Qatar > Direct Line +974-4404-2036 > jmoreira@sidra.org | www.sidra.org > > > > > > Disclaimer: This email and its attachments may be confidential and are > intended solely for the use of the individual to whom it is addressed. If > you are not the intended recipient, any reading, printing, storage, > disclosure, copying or any other action taken in respect of this e-mail is > prohibited and may be unlawful. If you are not the intended recipient, > please notify the sender immediately by using the reply function and then > permanently delete what you have received. Any views or opinions expressed > are solely those of the author and do not necessarily represent those of > Sidra Medical and Research Center. > > From dhanson <@t> stagnes.org Thu Apr 16 16:00:42 2015 From: dhanson <@t> stagnes.org (Hanson, Dawn) Date: Thu Apr 16 16:00:54 2015 Subject: [Histonet] RE: IHC Billing Questions & Controls Message-ID: <2C23FEE38F1CA0419B32E8D629B08E67457EA8EE@TX1P03DAG0207.apptixhealth.net> Brandy & Joana Controls are never billable, a control test does not produce a usable result based on the patient's specimen. The results of control tests - positive or negative only tell you that your reagents, stains, antibodies etc. are performing as expected. | Dawn Hanson | Lab Manager | Outreach | Saint Agnes Hospital, Baltimore, MD | | * p: 410-368-3083 | * dhanson@stagnes.org | ? www.stagnes.org | CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From JMacDonald <@t> mtsac.edu Thu Apr 16 16:21:12 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Apr 16 16:21:19 2015 Subject: [Histonet] Histo jobs in the Eureka area Message-ID: I have a student that will graduate in June with eligibility to sit for the HT exam. She will be looking for a job in the Eureka area. Does anyone know about the HT employment outlook there? Thank you, Jennifer MacDonald Sent from my iPhone From JMacDonald <@t> mtsac.edu Thu Apr 16 20:08:32 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Apr 16 20:08:22 2015 Subject: [Histonet] CSH Hotel deadline Message-ID: CSH rate at the hotel ends tonight. If you plan on attending the CSH please make your hotel reservations. Lots of great workshops and beautiful location. http://californiahistology.org/events.html From wtaylor8660 <@t> gmail.com Thu Apr 16 20:57:17 2015 From: wtaylor8660 <@t> gmail.com (T Williams) Date: Thu Apr 16 20:57:20 2015 Subject: [Histonet] Orange peel for GMS Message-ID: I tried using a moldy orange peel as a GMS control, but the staining did not work. I ran it in a 10 hour processing protocol. Should I try something different? Any suggestions would be extremely helpful - we're desperate for a GMS control. Thanks, T. Williams, HTL (ASCP), QIHC From leila.etemadi <@t> med.lu.se Fri Apr 17 03:53:59 2015 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Fri Apr 17 03:54:07 2015 Subject: [Histonet] ROI measurments Message-ID: <37E60058-7281-4C56-83D4-B15F4299F892@med.lu.se> Hi Histoland, I have some analysis problem, wonder if any of you out there has suggestions??? I am analysing my spinal cord images with NIS Elements software, using RIO function. I would like to be able to include or exclude some parts within the ROI area. I can?t find my way in this program. Also, I gave a try to imageJ, still struggling... Any input will be appreciated deeply! Wish you all a fantastic day! Leila :-) From jpiche <@t> wtbyhosp.org Fri Apr 17 06:10:54 2015 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Fri Apr 17 06:10:58 2015 Subject: [Histonet] Orange peel for GMS In-Reply-To: References: Message-ID: <631955447A364B45B9458D2905635110D8BD8FFD@WIN08-MBX-01.wtbyhosp.org> I got a nice GMS control from some moldy chicken in my fridge. I am also trying the onion. I haven't stained the onion yet but it actually cut beautifully so I have high hopes. I let you know how it turns out. Have a nice weekend! Jessica Piche, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of T Williams Sent: Thursday, April 16, 2015 9:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Orange peel for GMS I tried using a moldy orange peel as a GMS control, but the staining did not work. I ran it in a 10 hour processing protocol. Should I try something different? Any suggestions would be extremely helpful - we're desperate for a GMS control. Thanks, T. Williams, HTL (ASCP), QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From talulahgosh <@t> gmail.com Fri Apr 17 07:41:00 2015 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Fri Apr 17 07:41:03 2015 Subject: [Histonet] setting up for staining Message-ID: Hello! My lab doesn't really do staining (H and E, Masson Trichrome) too often (maybe once a week), but I'd really like to have a set of dishes with all of the plastic bins and what not. Unfortunately, this costs $500 for just one row. Has anyone tried the newer set-ups like this https://us.vwr.com/store/catalog/product.jsp?product_id=4790248 It's remarkably cheaper, but I wonder if that's because it's not as good. We don't have a lot of money, and convincing my boss to even consider this will probably be difficult. I've been using three glass dishes and pouring reagents in and out of them; it's time to move on to an actual set where we can store the reagents in the dish. While I wish we could buy the cool Tissue Tek version (since everyone else has it), it's not feasible at all considering the cost. (Also, Ann, stop lurking I know you're reading this!!) Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" From bcooper <@t> chla.usc.edu Fri Apr 17 08:31:26 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Fri Apr 17 08:31:31 2015 Subject: [Histonet] setting up for staining In-Reply-To: References: Message-ID: We use the same setup for our cytology staining. We run a Pap stain about once every three weeks. Those dishes and racks are sturdy. Thanks, Brian Cooper, HT (ASCP) Histology Supervisor, Path & Lab Medicine Children's Hospital, Los Angeles Sent from my Galaxy S3, so please forgive any weird typos . . . -----Original Message----- From: Emily Brown [talulahgosh@gmail.com] Received: Friday, 17 Apr 2015, 5:43AM To: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu] Subject: [Histonet] setting up for staining Hello! My lab doesn't really do staining (H and E, Masson Trichrome) too often (maybe once a week), but I'd really like to have a set of dishes with all of the plastic bins and what not. Unfortunately, this costs $500 for just one row. Has anyone tried the newer set-ups like this https://us.vwr.com/store/catalog/product.jsp?product_id=4790248 It's remarkably cheaper, but I wonder if that's because it's not as good. We don't have a lot of money, and convincing my boss to even consider this will probably be difficult. I've been using three glass dishes and pouring reagents in and out of them; it's time to move on to an actual set where we can store the reagents in the dish. While I wish we could buy the cool Tissue Tek version (since everyone else has it), it's not feasible at all considering the cost. (Also, Ann, stop lurking I know you're reading this!!) Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From akbitting <@t> geisinger.edu Fri Apr 17 10:21:00 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Fri Apr 17 10:21:08 2015 Subject: [Histonet] Orange peel for GMS In-Reply-To: <631955447A364B45B9458D2905635110D8BD8FFD@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110D8BD8FFD@WIN08-MBX-01.wtbyhosp.org> Message-ID: <5353e1f2607248c8a48bea94d41a37a4@GHSEXMBX4W12V.geisinger.edu> We just tried a moldy strawberry. It was loaded with fungus! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Friday, April 17, 2015 7:11 AM To: 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS I got a nice GMS control from some moldy chicken in my fridge. I am also trying the onion. I haven't stained the onion yet but it actually cut beautifully so I have high hopes. I let you know how it turns out. Have a nice weekend! Jessica Piche, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of T Williams Sent: Thursday, April 16, 2015 9:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Orange peel for GMS I tried using a moldy orange peel as a GMS control, but the staining did not work. I ran it in a 10 hour processing protocol. Should I try something different? Any suggestions would be extremely helpful - we're desperate for a GMS control. Thanks, T. Williams, HTL (ASCP), QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From lblazek <@t> digestivespecialists.com Fri Apr 17 11:05:17 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Apr 17 11:05:24 2015 Subject: [Histonet] Orange peel for GMS In-Reply-To: <5353e1f2607248c8a48bea94d41a37a4@GHSEXMBX4W12V.geisinger.edu> References: <631955447A364B45B9458D2905635110D8BD8FFD@WIN08-MBX-01.wtbyhosp.org> <5353e1f2607248c8a48bea94d41a37a4@GHSEXMBX4W12V.geisinger.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E39173CBFBF9E@IBMB7Exchange.digestivespecialists.com> Our blue cheese GMS would make you never want to eat it again! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Friday, April 17, 2015 11:21 AM To: Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS We just tried a moldy strawberry. It was loaded with fungus! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Friday, April 17, 2015 7:11 AM To: 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS I got a nice GMS control from some moldy chicken in my fridge. I am also trying the onion. I haven't stained the onion yet but it actually cut beautifully so I have high hopes. I let you know how it turns out. Have a nice weekend! Jessica Piche, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of T Williams Sent: Thursday, April 16, 2015 9:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Orange peel for GMS I tried using a moldy orange peel as a GMS control, but the staining did not work. I ran it in a 10 hour processing protocol. Should I try something different? Any suggestions would be extremely helpful - we're desperate for a GMS control. Thanks, T. Williams, HTL (ASCP), QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From jpiche <@t> wtbyhosp.org Fri Apr 17 11:05:53 2015 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Fri Apr 17 11:06:02 2015 Subject: [Histonet] Orange peel for GMS In-Reply-To: <5353e1f2607248c8a48bea94d41a37a4@GHSEXMBX4W12V.geisinger.edu> References: <631955447A364B45B9458D2905635110D8BD8FFD@WIN08-MBX-01.wtbyhosp.org> <5353e1f2607248c8a48bea94d41a37a4@GHSEXMBX4W12V.geisinger.edu> Message-ID: <631955447A364B45B9458D2905635110D8BDB93B@WIN08-MBX-01.wtbyhosp.org> Yeah I've tried that as well but the strawberries didn't cut as well as I'd like. The chicken is nice because its muscle but tends to require a lot of soaking. -----Original Message----- From: Bitting, Angela K. [mailto:akbitting@geisinger.edu] Sent: Friday, April 17, 2015 11:21 AM To: Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS We just tried a moldy strawberry. It was loaded with fungus! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Friday, April 17, 2015 7:11 AM To: 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS I got a nice GMS control from some moldy chicken in my fridge. I am also trying the onion. I haven't stained the onion yet but it actually cut beautifully so I have high hopes. I let you know how it turns out. Have a nice weekend! Jessica Piche, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of T Williams Sent: Thursday, April 16, 2015 9:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Orange peel for GMS I tried using a moldy orange peel as a GMS control, but the staining did not work. I ran it in a 10 hour processing protocol. Should I try something different? Any suggestions would be extremely helpful - we're desperate for a GMS control. Thanks, T. Williams, HTL (ASCP), QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From jpiche <@t> wtbyhosp.org Fri Apr 17 11:54:52 2015 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Fri Apr 17 11:54:56 2015 Subject: [Histonet] Orange peel for GMS In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39173CBFBF9E@IBMB7Exchange.digestivespecialists.com> References: <631955447A364B45B9458D2905635110D8BD8FFD@WIN08-MBX-01.wtbyhosp.org> <5353e1f2607248c8a48bea94d41a37a4@GHSEXMBX4W12V.geisinger.edu> <5A2BD13465E061429D6455C8D6B40E39173CBFBF9E@IBMB7Exchange.digestivespecialists.com> Message-ID: <631955447A364B45B9458D2905635110D8BDB955@WIN08-MBX-01.wtbyhosp.org> Hahahaha I bet not Linda!!! Jessica -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, April 17, 2015 12:05 PM To: Bitting, Angela K.; Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS Our blue cheese GMS would make you never want to eat it again! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Friday, April 17, 2015 11:21 AM To: Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS We just tried a moldy strawberry. It was loaded with fungus! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Friday, April 17, 2015 7:11 AM To: 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS I got a nice GMS control from some moldy chicken in my fridge. I am also trying the onion. I haven't stained the onion yet but it actually cut beautifully so I have high hopes. I let you know how it turns out. Have a nice weekend! Jessica Piche, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of T Williams Sent: Thursday, April 16, 2015 9:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Orange peel for GMS I tried using a moldy orange peel as a GMS control, but the staining did not work. I ran it in a 10 hour processing protocol. Should I try something different? Any suggestions would be extremely helpful - we're desperate for a GMS control. Thanks, T. Williams, HTL (ASCP), QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From loreli173 <@t> mac.com Fri Apr 17 12:24:25 2015 From: loreli173 <@t> mac.com (Lori GEMEINHARDT) Date: Fri Apr 17 12:24:53 2015 Subject: [Histonet] setting up for staining In-Reply-To: References: Message-ID: I am very interested in everyone's experience with this as well. I was just (this week!) looking into this set for performing FNA's in a doctors' office. Thank you in advance. :) > On Apr 17, 2015, at 8:41 AM, Emily Brown wrote: > > Hello! > > My lab doesn't really do staining (H and E, Masson Trichrome) too often > (maybe once a week), but I'd really like to have a set of dishes with all > of the plastic bins and what not. Unfortunately, this costs $500 for just > one row. > Has anyone tried the newer set-ups like this > https://us.vwr.com/store/catalog/product.jsp?product_id=4790248 > > It's remarkably cheaper, but I wonder if that's because it's not as good. > We don't have a lot of money, and convincing my boss to even consider this > will probably be difficult. I've been using three glass dishes and pouring > reagents in and out of them; it's time to move on to an actual set where we > can store the reagents in the dish. > While I wish we could buy the cool Tissue Tek version (since everyone else > has it), it's not feasible at all considering the cost. > > (Also, Ann, stop lurking I know you're reading this!!) > > Emily > > > "By bitching and bitching and bitching, they could exhaust the drama of > their own horror stories. Grow bored. Only then could they accept a new > story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lguernsey <@t> ucsd.edu Fri Apr 17 13:54:39 2015 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Fri Apr 17 13:55:22 2015 Subject: [Histonet] Will dry ice damage paraffin blocks? Message-ID: Hi Histonet, I need to ship some paraffin blocks and frozen tissue internationally to the same location. My instinct tells me that I need to ship the paraffin separately (at room temperature) from the dry ice package. But, now I'm wondering if that's really necessary. Should I ship 2 packages or can I place a layer of paper towels or something in between the dry ice and paraffin blocks? Thanks, Lucie Lucie Guernsey UC San Diego Dept. of Pathology lguernsey@ucsd.edu From contact <@t> excaliburpathology.com Fri Apr 17 13:56:33 2015 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Apr 17 13:59:39 2015 Subject: [Histonet] setting up for staining In-Reply-To: References: Message-ID: <1817568636.6435179.1429296993513.JavaMail.yahoo@mail.yahoo.com> I have used it in the past. Nice setup for low volumes.?Paula Pierce,BS, HTL(ASCP)HT?President?Excalibur Pathology, Inc.?5830 N Blue Lake Dr.?Norman, OK 73069?405-759-3953 PH 405-759-7513 FAX?www.excaliburpathology.com From: Lori GEMEINHARDT To: Emily Brown Cc: "histonet@lists.utsouthwestern.edu" Sent: Friday, April 17, 2015 12:24 PM Subject: Re: [Histonet] setting up for staining I am very interested in everyone's experience with this as well. I was just (this week!) looking into this set for performing FNA's in a doctors' office. Thank you in advance. :) > On Apr 17, 2015, at 8:41 AM, Emily Brown wrote: > > Hello! > > My lab doesn't really do staining (H and E, Masson Trichrome) too often > (maybe once a week), but I'd really like to have a set of dishes with all > of the plastic bins and what not.? Unfortunately, this costs $500 for just > one row. > Has anyone tried the newer set-ups like this > https://us.vwr.com/store/catalog/product.jsp?product_id=4790248 > > It's remarkably cheaper, but I wonder if that's because it's not as good. > We don't have a lot of money, and convincing my boss to even consider this > will probably be difficult.? I've been using three glass dishes and pouring > reagents in and out of them; it's time to move on to an actual set where we > can store the reagents in the dish. > While I wish we could buy the cool Tissue Tek version (since everyone else > has it), it's not feasible at all considering the cost. > > (Also, Ann, stop lurking I know you're reading this!!) > > Emily > > > "By bitching and bitching and bitching, they could exhaust the drama of > their own horror stories. Grow bored. Only then could they accept a new > story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ltougas <@t> dawsoncollege.qc.ca Fri Apr 17 14:04:22 2015 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Fri Apr 17 14:04:28 2015 Subject: [Histonet] NSH region IX symposium Message-ID: <455897B94CF3A44F81F727160F23FB11640C14C5@DC229.ad.dawsoncollege.qc.ca> Histology labs - screaming for automation NSH Region IX Symposium June 5th-6TH, 2015 Hilton Garden Inn 3311 Caroga Drive, Mississauga ON Join us for stimulating educational sessions for Histotechnologists, Pathologists, Vendors, Students and other Healthcare Professionals. Online Registration Opening April 6th Members $100, Non-Members $150, Students $10 Wine and Cheese with Keynote Speaker Friday June 5th from 7:30-9:00 PM Highlights ? Interactive education sessions ? Meet with Industry representatives ? Interact and network with peers ? Following the IQMH Symposium For more information visit nshregionix.org/education Or contact Jshin@gmail.com From b-frederick <@t> northwestern.edu Fri Apr 17 14:22:58 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Apr 17 14:23:04 2015 Subject: [Histonet] Schiffs Message-ID: Am I missing something? I ordered Schiffs and sigma tells me it was shipped yesterday. Hello, today is Friday and last I recall Schiffs need to be refrigerated! You'd...... think they would realize this. Sorry all, have to vent..... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From rachel <@t> gbi-inc.com Fri Apr 17 15:21:06 2015 From: rachel <@t> gbi-inc.com (Rachel M Gonzalez) Date: Fri Apr 17 15:21:09 2015 Subject: [Histonet] UKNEQAS IHC participants Help does anyone have the Identity number for Run 109 Message-ID: Hi Does anyone know the Identity number for NSCLC ALK IHC Module.109 the paper work does not have it. I need to submit information midnight and it is to late to contact UK Thanks in advance for your help Rachel From amosbrooks <@t> gmail.com Fri Apr 17 15:30:45 2015 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Apr 17 15:30:52 2015 Subject: [Histonet] setting up for staining Message-ID: Hi, It's a good setup for a smaller volume lab. The racks hold 10 slides and the dishes slide together and link into a chain or can be used separately. I have used the dishes for antigen retrieval and they haven't cracked or warped. I haven't regretted the purchase. Cheers, Amos Brooks On Fri, Apr 17, 2015 at 1:00 PM, wrote: > Message: 8 > Date: Fri, 17 Apr 2015 08:41:00 -0400 > From: Emily Brown > Subject: [Histonet] setting up for staining > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > xg4YyFjWafkiTAWA@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Hello! > > My lab doesn't really do staining (H and E, Masson Trichrome) too often > (maybe once a week), but I'd really like to have a set of dishes with all > of the plastic bins and what not. Unfortunately, this costs $500 for just > one row. > Has anyone tried the newer set-ups like this > https://us.vwr.com/store/catalog/product.jsp?product_id=4790248 > > It's remarkably cheaper, but I wonder if that's because it's not as good. > We don't have a lot of money, and convincing my boss to even consider this > will probably be difficult. I've been using three glass dishes and pouring > reagents in and out of them; it's time to move on to an actual set where we > can store the reagents in the dish. > While I wish we could buy the cool Tissue Tek version (since everyone else > has it), it's not feasible at all considering the cost. > > (Also, Ann, stop lurking I know you're reading this!!) > > Emily > From jkiernan <@t> uwo.ca Sat Apr 18 01:26:34 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Apr 18 01:26:40 2015 Subject: [Histonet] Schiffs In-Reply-To: <7360e5f21cd5.5531f8d4@uwo.ca> References: <73e0b41414f6.5531f6a7@uwo.ca> <73c0a35e5592.5531f6e3@uwo.ca> <7350cb6742c4.5531f721@uwo.ca> <7370b63f44c7.5531f75f@uwo.ca> <73e09ed61c84.5531f79d@uwo.ca> <73e0be171b61.5531f7db@uwo.ca> <73c0e9fd51ed.5531f819@uwo.ca> <7370b65abb5.5531f858@uwo.ca> <7340bba32f7d.5531f896@uwo.ca> <7360e5f21cd5.5531f8d4@uwo.ca> Message-ID: <736082fa3941.5531b2ca@uwo.ca> Schiff's reagent does not need to be refrigerated. It just needs to be screw-capped so that it doesn't decompose by loss of sulphur dioxide. For decades, Schiff has been stored in many labs at 4C for no good reason. It is used at room temperature in both of its histochemical applications: the Feulgen and PAS reactions. John Kiernan Anatomy, UWO London, Canada = = = On 17/04/15, Bernice Frederick wrote: > Am I missing something? I ordered Schiffs and sigma tells me it was shipped yesterday. Hello, today is Friday and last I recall Schiffs need to be refrigerated! You'd...... > think they would realize this. Sorry all, have to vent..... > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From joelleweaver <@t> hotmail.com Sat Apr 18 05:39:35 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Sat Apr 18 05:39:40 2015 Subject: [Histonet] RE: IHC Billing Question In-Reply-To: References: , , Message-ID: No charge to patient account for negative or positive controls. Only the patient test (s). The controls verify that the test ran accurately, and for interpretation. You just have to figure this into your cost/test. As many have already posted in this string it is why many labs aside from QC reasons, have adopted the single slide for postive/patient and have also eliminated negatives when polymer detection is used. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mward@wakehealth.edu > To: bburnett@CapeCodHealth.org; jmoreira@sidra.org; histonet@lists.utsouthwestern.edu > Date: Thu, 16 Apr 2015 14:35:59 +0000 > CC: > Subject: [Histonet] RE: IHC Billing Question > > We have not been charging for the negative control, assuming that it was just a cost of doing business. I would be interested to hear if anyone has been charging for their negative controls as well. > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 > p 336.716.2109 \ f 336.716.5890 > mward@wakehealth.edu > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy > Sent: Thursday, April 16, 2015 9:48 AM > To: 'Joana Moreira'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: IHC Billing Question > > We recently added HER2 IHC testing in our lab which we are required to use a negative reagent control For each case. Is there a cpt code for negative reagent control reimbursement? Any information on this Would be much appreciated! > Thanks > > Brandy Burnett > Histotechnoligist, QIHC(ASCP) > CCH Pathology/Histology > > > Expert physicians. Quality hospitals. Superior care. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joana Moreira > Sent: Thursday, April 16, 2015 7:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC Billing Question > > Greetings from Doha! > > This was much probably discussed before, but I was wondering if you could help me with a query in regards to billing. > For the sites that are still doing an IHC negative reagent control for each patient specimen, do you bill for the negative control? Using code 88341? > > I believe it should be billed (since when and if performed correctly the negative control follows a normal IHC technique) however I am completely new to the billing subject. My previous experience is based in Portugal and UK (where billing does not exist) and I've been introduced to this topic since I joined my current institution that will be following the North American Healthcare model. So... any help will be GREATLY appreciated!! > > Many Thanks in advance, > Joana > > Joana Moreira > Supervisor - Anatomical Pathology > Department of Pathology > > Sidra Medical & Research Center > PO Box 26999 | Doha, Qatar > Direct Line +974-4404-2036 > jmoreira@sidra.org | www.sidra.org > > > > > > Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk@CapeCodHealth.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Apr 18 05:45:42 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Sat Apr 18 05:45:46 2015 Subject: [Histonet] Schiffs In-Reply-To: <736082fa3941.5531b2ca@uwo.ca> References: , <73e0b41414f6.5531f6a7@uwo.ca> <73c0a35e5592.5531f6e3@uwo.ca>,<7350cb6742c4.5531f721@uwo.ca> <7370b63f44c7.5531f75f@uwo.ca>,<73e09ed61c84.5531f79d@uwo.ca> <73e0be171b61.5531f7db@uwo.ca>,<73c0e9fd51ed.5531f819@uwo.ca> <7370b65abb5.5531f858@uwo.ca>,<7340bba32f7d.5531f896@uwo.ca> <7360e5f21cd5.5531f8d4@uwo.ca>,<736082fa3941.5531b2ca@uwo.ca> Message-ID: The reagent we use for our manual backup stains using Schiffs states refrigeration for long term storage only. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jkiernan@uwo.ca > To: b-frederick@northwestern.edu; histonet@lists.utsouthwestern.edu > Date: Sat, 18 Apr 2015 01:26:34 -0500 > Subject: Re: [Histonet] Schiffs > CC: > > Schiff's reagent does not need to be refrigerated. It just needs to be screw-capped so that it doesn't decompose by loss of sulphur dioxide. For decades, Schiff has been stored in many labs at 4C for no good reason. It is used at room temperature in both of its histochemical applications: the Feulgen and PAS reactions. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > On 17/04/15, Bernice Frederick wrote: > > Am I missing something? I ordered Schiffs and sigma tells me it was shipped yesterday. Hello, today is Friday and last I recall Schiffs need to be refrigerated! You'd...... > > think they would realize this. Sorry all, have to vent..... > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From blayjorge <@t> gmail.com Sat Apr 18 09:31:17 2015 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Sat Apr 18 09:31:20 2015 Subject: [Histonet] LEB 3(1) freely available http://blaypublishers.com/2015/04/18/leb-31-2015/, includes paper of histological interest - Jorge Message-ID: http://blaypublishers.com/2015/04/18/leb-31-2015/ -- Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From algranth <@t> email.arizona.edu Sat Apr 18 09:46:45 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Sat Apr 18 09:46:49 2015 Subject: [Histonet] Schiffs In-Reply-To: References: , <73e0b41414f6.5531f6a7@uwo.ca> <73c0a35e5592.5531f6e3@uwo.ca>,<7350cb6742c4.5531f721@uwo.ca> <7370b63f44c7.5531f75f@uwo.ca>,<73e09ed61c84.5531f79d@uwo.ca> <73e0be171b61.5531f7db@uwo.ca>,<73c0e9fd51ed.5531f819@uwo.ca> <7370b65abb5.5531f858@uwo.ca>,<7340bba32f7d.5531f896@uwo.ca> <7360e5f21cd5.5531f8d4@uwo.ca>, <736082fa3941.5531b2ca@uwo.ca>, Message-ID: I bought Schiff's from Newcomer and it could be stored at RT. Andi G. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Joelle Weaver [joelleweaver@hotmail.com] Sent: Saturday, April 18, 2015 3:45 AM To: John Kiernan; Bernice Frederick; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Schiffs The reagent we use for our manual backup stains using Schiffs states refrigeration for long term storage only. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jkiernan@uwo.ca > To: b-frederick@northwestern.edu; histonet@lists.utsouthwestern.edu > Date: Sat, 18 Apr 2015 01:26:34 -0500 > Subject: Re: [Histonet] Schiffs > CC: > > Schiff's reagent does not need to be refrigerated. It just needs to be screw-capped so that it doesn't decompose by loss of sulphur dioxide. For decades, Schiff has been stored in many labs at 4C for no good reason. It is used at room temperature in both of its histochemical applications: the Feulgen and PAS reactions. > > John Kiernan > Anatomy, UWO > London, Canada > = = = > On 17/04/15, Bernice Frederick wrote: > > Am I missing something? I ordered Schiffs and sigma tells me it was shipped yesterday. Hello, today is Friday and last I recall Schiffs need to be refrigerated! You'd...... > > think they would realize this. Sorry all, have to vent..... > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcman2010 <@t> hotmail.com Sun Apr 19 03:26:29 2015 From: ihcman2010 <@t> hotmail.com (Glen) Date: Sun Apr 19 03:48:38 2015 Subject: [Histonet] From: Glen Message-ID: <29851A3C-FD4A-41CA-A0F4-2704C94C20AE@bombril.com.br> Hey histonet http://sitespoiler.com/brilliant.php?cross=ngptsne69gv60n ihcman2010@hotmail.com Sent from my iPhone From linda.prasad <@t> health.nsw.gov.au Sun Apr 19 18:57:21 2015 From: linda.prasad <@t> health.nsw.gov.au (Linda Prasad (SCHN)) Date: Sun Apr 19 18:57:53 2015 Subject: [Histonet] Orange peel for GMS In-Reply-To: <631955447A364B45B9458D2905635110D8BDB955@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110D8BD8FFD@WIN08-MBX-01.wtbyhosp.org> <5353e1f2607248c8a48bea94d41a37a4@GHSEXMBX4W12V.geisinger.edu> <5A2BD13465E061429D6455C8D6B40E39173CBFBF9E@IBMB7Exchange.digestivespecialists.com> <631955447A364B45B9458D2905635110D8BDB955@WIN08-MBX-01.wtbyhosp.org> Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E8BF64@xmdb03.nch.kids> What about plain boiled rice. Leave if out for a few days. You will get a lot of mould growing. Rice should be easy to cut as well. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Saturday, 18 April 2015 2:55 AM To: 'Blazek, Linda'; Bitting, Angela K.; 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS Hahahaha I bet not Linda!!! Jessica -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, April 17, 2015 12:05 PM To: Bitting, Angela K.; Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS Our blue cheese GMS would make you never want to eat it again! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Friday, April 17, 2015 11:21 AM To: Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS We just tried a moldy strawberry. It was loaded with fungus! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Friday, April 17, 2015 7:11 AM To: 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS I got a nice GMS control from some moldy chicken in my fridge. I am also trying the onion. I haven't stained the onion yet but it actually cut beautifully so I have high hopes. I let you know how it turns out. Have a nice weekend! Jessica Piche, HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of T Williams Sent: Thursday, April 16, 2015 9:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Orange peel for GMS I tried using a moldy orange peel as a GMS control, but the staining did not work. I ran it in a 10 hour processing protocol. Should I try something different? Any suggestions would be extremely helpful - we're desperate for a GMS control. Thanks, T. Williams, HTL (ASCP), QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tjfinney2010 <@t> gmail.com Sun Apr 19 19:24:53 2015 From: tjfinney2010 <@t> gmail.com (=?utf-8?B?dGpmaW5uZXkyMDEwQGdtYWlsLmNvbQ==?=) Date: Sun Apr 19 19:24:58 2015 Subject: [Histonet] (no subject) Message-ID: <000f425a.0a664de4196a9639@gmail.com> GMS controls From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting. Sent from my Sprint Phone. From garreyf <@t> gmail.com Sun Apr 19 19:43:48 2015 From: garreyf <@t> gmail.com (Garreyf) Date: Sun Apr 19 19:43:57 2015 Subject: [Histonet] Orange peel for GMS In-Reply-To: <1217DDB3D7DE5E418E3D560A268EABD0E0E8BF64@xmdb03.nch.kids> References: <631955447A364B45B9458D2905635110D8BD8FFD@WIN08-MBX-01.wtbyhosp.org> <5353e1f2607248c8a48bea94d41a37a4@GHSEXMBX4W12V.geisinger.edu> <5A2BD13465E061429D6455C8D6B40E39173CBFBF9E@IBMB7Exchange.digestivespecialists.com> <631955447A364B45B9458D2905635110D8BDB955@WIN08-MBX-01.wtbyhosp.org> <1217DDB3D7DE5E418E3D560A268EABD0E0E8BF64@xmdb03.nch.kids> Message-ID: <83D1275F-5757-454D-B7FE-585ACF640823@gmail.com> We did the slim Jim thing for the gram control and it grew fungus. It works great as a fungus control . I haven't tested it yet for bacteria. I may have a dual control! Garrey Sent from my iPhone > On Apr 19, 2015, at 7:57 PM, Linda Prasad (SCHN) wrote: > > What about plain boiled rice. Leave if out for a few days. You will get a lot of mould growing. Rice should be easy to cut as well. > > Linda Prasad | Senior Scientist | Histopathology > t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au > > Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia > Locked Bag 4001, Westmead 2145, NSW Australia > ? Please consider the environment before printing this email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica > Sent: Saturday, 18 April 2015 2:55 AM > To: 'Blazek, Linda'; Bitting, Angela K.; 'T Williams'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Orange peel for GMS > > Hahahaha I bet not Linda!!! > > Jessica > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda > Sent: Friday, April 17, 2015 12:05 PM > To: Bitting, Angela K.; Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Orange peel for GMS > > Our blue cheese GMS would make you never want to eat it again! > > Linda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. > Sent: Friday, April 17, 2015 11:21 AM > To: Piche, Jessica; 'T Williams'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Orange peel for GMS > > We just tried a moldy strawberry. It was loaded with fungus! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica > Sent: Friday, April 17, 2015 7:11 AM > To: 'T Williams'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Orange peel for GMS > > I got a nice GMS control from some moldy chicken in my fridge. I am also trying the onion. I haven't stained the onion yet but it actually cut beautifully so I have high hopes. I let you know how it turns out. Have a nice weekend! > > Jessica Piche, HT(ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of T Williams > Sent: Thursday, April 16, 2015 9:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Orange peel for GMS > > I tried using a moldy orange peel as a GMS control, but the staining did not work. I ran it in a 10 hour processing protocol. Should I try something different? Any suggestions would be extremely helpful - we're desperate for a GMS control. > > Thanks, > T. Williams, HTL (ASCP), QIHC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Sun Apr 19 20:06:46 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Apr 19 20:07:02 2015 Subject: [Histonet] (no subject) In-Reply-To: <000f425a.0a664de4196a9639@gmail.com> References: <000f425a.0a664de4196a9639@gmail.com> Message-ID: <1039994919.6475395.1429492006874.JavaMail.zimbra@comcast.net> I asked about this in a different vein months ago.? Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from?the inspector?either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA ----- Original Message ----- From: tjfinney2010@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls >From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting. ?Sent from my Sprint Phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PREISZNE <@t> mail.etsu.edu Mon Apr 20 10:47:09 2015 From: PREISZNE <@t> mail.etsu.edu (Preiszner, Johanna) Date: Mon Apr 20 10:47:20 2015 Subject: [Histonet] IHC and oven temperature Message-ID: Hi Netters, is there something wrong with this logic: "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... Thanks! Hanna Preiszner ETSU/QCOM From Julia.Cates <@t> AHSS.ORG Mon Apr 20 10:51:06 2015 From: Julia.Cates <@t> AHSS.ORG (Cates, Julia) Date: Mon Apr 20 10:51:13 2015 Subject: [Histonet] setting up for staining Message-ID: We have used this set up for several years now for CT biopsies. We like the setup because it is portable ( it is contained in a carrier for transport). The reagents are good for about 3-4 uses (we use it rapidly so contamination occurs quickly) and then it must be changed. The stains are changed less frequently. My only complaint is that the description says the containers can be "loosely" joined but you may as well disregard that. The containers do not stay together at all. Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. From Timothy.Morken <@t> ucsf.edu Mon Apr 20 10:52:53 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Mon Apr 20 10:53:00 2015 Subject: [Histonet] RE: IHC and oven temperature In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF36805764@ex07.net.ucsf.edu> The95 for HIER is in liquid, The 82in the oven is dry slides. While wet high temps enhance HIER, It seems the high dry temp does harm epitopes (there is a paper somewhere on this but I don't have access to it right now). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Preiszner, Johanna Sent: Monday, April 20, 2015 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and oven temperature Hi Netters, is there something wrong with this logic: "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... Thanks! Hanna Preiszner ETSU/QCOM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Mon Apr 20 10:56:10 2015 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon Apr 20 10:56:18 2015 Subject: [Histonet] IHC and oven temperature In-Reply-To: References: Message-ID: Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes Sent from my iPhone > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > Hi Netters, > > is there something wrong with this logic: > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > Thanks! > > Hanna Preiszner > ETSU/QCOM > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rachel <@t> gbi-inc.com Mon Apr 20 11:07:29 2015 From: rachel <@t> gbi-inc.com (Rachel M Gonzalez) Date: Mon Apr 20 11:07:31 2015 Subject: [Histonet] prevent wrinkles when cutting Message-ID: Hi Thursday was the first time I ever used a microtome.... I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no problems getting ribbons of 10-30 sections long but the pieces are half the size of the original block. I am guessing they are wrinkling. What am I doing wrong? Thanks Rachel Senior Scientist.... From amurvosh <@t> advancederm.net Mon Apr 20 11:25:33 2015 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Mon Apr 20 11:25:42 2015 Subject: [Histonet] prevent wrinkles when cutting In-Reply-To: References: Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7A820AD@Exchange.Advancederm.net> First make sure your block is cold. Second, some models of microtome's and blades don't cut well without dulling the blade a bit.(newer models don't seem to need it.) Lastly some processors don?t fix as well as others you may need to face the blocks in and soak them. Again some places soak in ice water alone, some use fabric softener on the ice and some use ammonia water. (that one is stinky). Some places do soaking at all. I now have a really old microtome and have to blow or breath on the paraffin lightly as I'm cutting, an old technique but effective. Sorry its going to be a lot of trial and error. Maybe you can call the previous tech on how they did it there. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rachel M Gonzalez Sent: Monday, April 20, 2015 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prevent wrinkles when cutting Hi Thursday was the first time I ever used a microtome.... I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no problems getting ribbons of 10-30 sections long but the pieces are half the size of the original block. I am guessing they are wrinkling. What am I doing wrong? Thanks Rachel Senior Scientist.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Mon Apr 20 11:26:44 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Apr 20 11:26:50 2015 Subject: [Histonet] prevent wrinkles when cutting In-Reply-To: References: Message-ID: <9d526885f6fc4caaa5b2a8019f26d250@MAIL13M2N2.ad.uams.edu> How thick are you cutting? Is the block cold? Are you using disposable knives? Are you moving the knife when you start sectioning? How fast are you attempting to cut? What kind of tissue are you cutting? Do you know which paraffin is being used? There are many reasons for wrinkles and these are few of the questions we need to answer first. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rachel M Gonzalez Sent: Monday, April 20, 2015 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prevent wrinkles when cutting Hi Thursday was the first time I ever used a microtome.... I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no problems getting ribbons of 10-30 sections long but the pieces are half the size of the original block. I am guessing they are wrinkling. What am I doing wrong? Thanks Rachel Senior Scientist.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From algranth <@t> email.arizona.edu Mon Apr 20 11:40:48 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Apr 20 11:41:15 2015 Subject: FW: [Histonet] prevent wrinkles when cutting In-Reply-To: References: , Message-ID: Rachel, First off, are you chilling and soaking the blocks after you face them? Do that and see if there is a difference. Don't try to get many sections to your ribbons. Shoot for a smaller ribbon (5-6) sections that are good. Cut slowly but consistently. What microtome are you using? Are you using disposable blades and are they sharp? Don't expect them to cut well if you use the same blade to face the blocks. If you aren't using disposables, get some! They will make your life easier. You might try to find a histotech at a local hospital lab who might be able to give you a hands-on lesson. Don't despair! We all sat down at our microtomes those first times and suffered trying to get perfect sections. It takes practice. You might make some blank blocks or blocks with tissue you can spare to practice your cutting techniques. I used to do this with my students and it really helped them. Good luck! Andi G. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rachel M Gonzalez [rachel@gbi-inc.com] Sent: Monday, April 20, 2015 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prevent wrinkles when cutting Hi Thursday was the first time I ever used a microtome.... I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no problems getting ribbons of 10-30 sections long but the pieces are half the size of the original block. I am guessing they are wrinkling. What am I doing wrong? Thanks Rachel Senior Scientist.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Mon Apr 20 12:04:27 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Apr 20 12:04:32 2015 Subject: [Histonet] prevent wrinkles when cutting In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632616787BF7@LRGHEXVS1.practice.lrgh.org> Check you blade angle. Keep your blocks cold, change your blade often and just be patient. Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rachel M Gonzalez Sent: Monday, April 20, 2015 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prevent wrinkles when cutting Hi Thursday was the first time I ever used a microtome.... I move to a lab that does not have someone dedicated to cutting. I already miss her. I have no problems getting ribbons of 10-30 sections long but the pieces are half the size of the original block. I am guessing they are wrinkling. What am I doing wrong? Thanks Rachel Senior Scientist.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From j.benavides <@t> eae.csic.es Mon Apr 20 12:06:17 2015 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Mon Apr 20 12:12:01 2015 Subject: FW: [Histonet] prevent wrinkles when cutting In-Reply-To: References: , Message-ID: <55353209.4020104@eae.csic.es> Hi there, I?m curious about the soaking thing. We have never done it in our lab. Which is the purpose to do it? Than, after facing the blocks, we chill them in a cold plate so, if wanting to do the soaking , when should we? I guess before placing them on the cold plate, but that may cause a bit of ice formation? Thanks a lot for your help Julio On 20/04/2015 18:40, Grantham, Andrea L - (algranth) wrote: > Rachel, > First off, are you chilling and soaking the blocks after you face them? > Do that and see if there is a difference. > Don't try to get many sections to your ribbons. Shoot for a smaller ribbon (5-6) sections that are good. Cut slowly but consistently. > What microtome are you using? Are you using disposable blades and are they sharp? Don't expect them to cut well if you use the same blade to face the blocks. If you aren't using disposables, get some! They will make your life easier. > You might try to find a histotech at a local hospital lab who might be able to give you a hands-on lesson. > Don't despair! We all sat down at our microtomes those first times and suffered trying to get perfect sections. It takes practice. You might make some blank blocks or blocks with tissue you can spare to practice your cutting techniques. I used to do this with my students and it really helped them. > Good luck! > > Andi G. > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rachel M Gonzalez [rachel@gbi-inc.com] > Sent: Monday, April 20, 2015 9:07 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] prevent wrinkles when cutting > > Hi > > Thursday was the first time I ever used a microtome.... I move to a lab > that does not have someone dedicated to cutting. I already miss her. > > I have no problems getting ribbons of 10-30 sections long but the pieces > are half the size of the original block. I am guessing they are wrinkling. > What am I doing wrong? > > Thanks > Rachel > Senior Scientist.... > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Mon Apr 20 12:35:37 2015 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon Apr 20 12:35:54 2015 Subject: [Histonet] prevent wrinkles when cutting In-Reply-To: <55353209.4020104@eae.csic.es> References: <55353209.4020104@eae.csic.es> Message-ID: If you process properly, no over dehydration, there is no reason to soak. Keeping block cool will help in cutting. Sharp, clean blade a must. Correct water bath temp and time are the most critical factors, after cutting, to produce a wrinkle free section. Sent from my iPhone > On Apr 20, 2015, at 10:12 AM, Julio Benavides wrote: > > Hi there, > > I?m curious about the soaking thing. We have never done it in our lab. Which is the purpose to do it? > > Than, after facing the blocks, we chill them in a cold plate so, if wanting to do the soaking , when should we? I guess before placing them on the cold plate, but that may cause a bit of ice formation? > > Thanks a lot for your help > > Julio > > >> On 20/04/2015 18:40, Grantham, Andrea L - (algranth) wrote: >> Rachel, >> First off, are you chilling and soaking the blocks after you face them? >> Do that and see if there is a difference. >> Don't try to get many sections to your ribbons. Shoot for a smaller ribbon (5-6) sections that are good. Cut slowly but consistently. >> What microtome are you using? Are you using disposable blades and are they sharp? Don't expect them to cut well if you use the same blade to face the blocks. If you aren't using disposables, get some! They will make your life easier. >> You might try to find a histotech at a local hospital lab who might be able to give you a hands-on lesson. >> Don't despair! We all sat down at our microtomes those first times and suffered trying to get perfect sections. It takes practice. You might make some blank blocks or blocks with tissue you can spare to practice your cutting techniques. I used to do this with my students and it really helped them. >> Good luck! >> >> Andi G. >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rachel M Gonzalez [rachel@gbi-inc.com] >> Sent: Monday, April 20, 2015 9:07 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] prevent wrinkles when cutting >> >> Hi >> >> Thursday was the first time I ever used a microtome.... I move to a lab >> that does not have someone dedicated to cutting. I already miss her. >> >> I have no problems getting ribbons of 10-30 sections long but the pieces >> are half the size of the original block. I am guessing they are wrinkling. >> What am I doing wrong? >> >> Thanks >> Rachel >> Senior Scientist.... >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Apr 20 14:28:47 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Mon Apr 20 14:28:52 2015 Subject: [Histonet] prevent wrinkles when cutting In-Reply-To: <55353209.4020104@eae.csic.es> References: , , , , <55353209.4020104@eae.csic.es> Message-ID: Why are you cutting such a long ribbon? You usually only a need a series of 3-4 sections even for ribbon cutting. Might be easier to control if you don't try to move such a long ribbon to the waterbath. Drag the shorter ribbon towards you on the waterbath. Make sure the water is not too cool. Face the block to full face but superficial, chill on ice for some moisture, take sections while the block is still very cold. Use a slow, steady, smooth stroke if doing manual cutting. Make sure your embedding works well for the way you orient the block in the holder. Angles work well for many tissues that are prone to wrinkling. Its mostly just practice though. The more you cut, the easier it becomes and usually the better you get at it. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 20 Apr 2015 19:06:17 +0200 > From: j.benavides@eae.csic.es > To: histonet@lists.utsouthwestern.edu > Subject: Re: FW: [Histonet] prevent wrinkles when cutting > > Hi there, > > I?m curious about the soaking thing. We have never done it in our lab. > Which is the purpose to do it? > > Than, after facing the blocks, we chill them in a cold plate so, if > wanting to do the soaking , when should we? I guess before placing them > on the cold plate, but that may cause a bit of ice formation? > > Thanks a lot for your help > > Julio > > > On 20/04/2015 18:40, Grantham, Andrea L - (algranth) wrote: > > Rachel, > > First off, are you chilling and soaking the blocks after you face them? > > Do that and see if there is a difference. > > Don't try to get many sections to your ribbons. Shoot for a smaller ribbon (5-6) sections that are good. Cut slowly but consistently. > > What microtome are you using? Are you using disposable blades and are they sharp? Don't expect them to cut well if you use the same blade to face the blocks. If you aren't using disposables, get some! They will make your life easier. > > You might try to find a histotech at a local hospital lab who might be able to give you a hands-on lesson. > > Don't despair! We all sat down at our microtomes those first times and suffered trying to get perfect sections. It takes practice. You might make some blank blocks or blocks with tissue you can spare to practice your cutting techniques. I used to do this with my students and it really helped them. > > Good luck! > > > > Andi G. > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rachel M Gonzalez [rachel@gbi-inc.com] > > Sent: Monday, April 20, 2015 9:07 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] prevent wrinkles when cutting > > > > Hi > > > > Thursday was the first time I ever used a microtome.... I move to a lab > > that does not have someone dedicated to cutting. I already miss her. > > > > I have no problems getting ribbons of 10-30 sections long but the pieces > > are half the size of the original block. I am guessing they are wrinkling. > > What am I doing wrong? > > > > Thanks > > Rachel > > Senior Scientist.... > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Apr 20 14:30:18 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Mon Apr 20 14:30:22 2015 Subject: [Histonet] prevent wrinkles when cutting In-Reply-To: <55353209.4020104@eae.csic.es> References: , , , , <55353209.4020104@eae.csic.es> Message-ID: No ice forms in fixed, paraffin embedded tissue blocks at usual temperatures and length of chilling time and temperatures. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 20 Apr 2015 19:06:17 +0200 > From: j.benavides@eae.csic.es > To: histonet@lists.utsouthwestern.edu > Subject: Re: FW: [Histonet] prevent wrinkles when cutting > > Hi there, > > I?m curious about the soaking thing. We have never done it in our lab. > Which is the purpose to do it? > > Than, after facing the blocks, we chill them in a cold plate so, if > wanting to do the soaking , when should we? I guess before placing them > on the cold plate, but that may cause a bit of ice formation? > > Thanks a lot for your help > > Julio > > > On 20/04/2015 18:40, Grantham, Andrea L - (algranth) wrote: > > Rachel, > > First off, are you chilling and soaking the blocks after you face them? > > Do that and see if there is a difference. > > Don't try to get many sections to your ribbons. Shoot for a smaller ribbon (5-6) sections that are good. Cut slowly but consistently. > > What microtome are you using? Are you using disposable blades and are they sharp? Don't expect them to cut well if you use the same blade to face the blocks. If you aren't using disposables, get some! They will make your life easier. > > You might try to find a histotech at a local hospital lab who might be able to give you a hands-on lesson. > > Don't despair! We all sat down at our microtomes those first times and suffered trying to get perfect sections. It takes practice. You might make some blank blocks or blocks with tissue you can spare to practice your cutting techniques. I used to do this with my students and it really helped them. > > Good luck! > > > > Andi G. > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rachel M Gonzalez [rachel@gbi-inc.com] > > Sent: Monday, April 20, 2015 9:07 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] prevent wrinkles when cutting > > > > Hi > > > > Thursday was the first time I ever used a microtome.... I move to a lab > > that does not have someone dedicated to cutting. I already miss her. > > > > I have no problems getting ribbons of 10-30 sections long but the pieces > > are half the size of the original block. I am guessing they are wrinkling. > > What am I doing wrong? > > > > Thanks > > Rachel > > Senior Scientist.... > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Mon Apr 20 14:52:57 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon Apr 20 14:53:13 2015 Subject: [Histonet] prevent wrinkles when cutting In-Reply-To: References: <55353209.4020104@eae.csic.es> Message-ID: <1417701948.2705897.1429559577837.JavaMail.zimbra@comcast.net> The one question we have not asked is; are you cutting serial sections for these blocks?? If you are are you laying them out on a board and not a waterbath?? I have done that with neuropath sections in research for whole rat and monkey brains.? Then your ribbon length would make sense.? Generally the longer the ribbon you have to handle the more likely you are to lose sections or have it fold over on its self.? When you are just learning mastering shorter ribbons is easier.? If you are going to a waterbath you can crowd the ribbon on the bath and it had no place to gently stretch out. ? Pam Marcum ----- Original Message ----- From: "Joelle Weaver" To: "Julio Benavides" , "Histonet" Sent: Monday, April 20, 2015 2:28:47 PM Subject: RE: [Histonet] prevent wrinkles when cutting Why are you cutting such a long ribbon? You usually only a need a series of 3-4 sections even for ribbon cutting. Might be easier to control if you don't try to move such a long ribbon to the waterbath. Drag the shorter ribbon towards you on the waterbath. Make sure the water is not too cool. Face the block to full face but superficial, chill on ice for some moisture, take sections while the block is still very cold. Use a slow, steady, smooth stroke if doing manual cutting. Make sure your embedding works well for the way you orient the block in the holder. Angles work well for many tissues that are prone to wrinkling. Its mostly just practice though. The more you cut, the easier it becomes and usually the better you get at it. Joelle Weaver MAOM, HTL (ASCP) QIHC ?? ? ? ? ?? ? > Date: Mon, 20 Apr 2015 19:06:17 +0200 > From: j.benavides@eae.csic.es > To: histonet@lists.utsouthwestern.edu > Subject: Re: FW: [Histonet] prevent wrinkles when cutting > > Hi there, > > I?m curious about the soaking thing. We have never done it in our lab. > Which is the purpose to do it? > > Than, after facing the blocks, we chill them in a cold plate so, if > wanting to do the soaking , when should we? I guess before placing them > on the cold plate, but that may cause a bit of ice formation? > > Thanks a lot for your help > > Julio > > > On 20/04/2015 18:40, Grantham, Andrea L - (algranth) wrote: > > Rachel, > > First off, are you chilling and soaking the blocks after you face them? > > Do that and see if there is a difference. > > Don't try to get many sections to your ribbons. Shoot for a smaller ribbon (5-6) sections that are good. Cut slowly but consistently. > > What microtome are you using? Are you using disposable blades and are they sharp? Don't expect them to cut well if you use the same blade to face the blocks. If you aren't using disposables, get some! They will make your life easier. > > You might try to find a histotech at a local hospital lab who might be able to give you a hands-on lesson. > > Don't despair! We all sat down at our microtomes those first times and suffered trying to get perfect sections. It takes practice. You might make some blank blocks or blocks with tissue you can spare to practice your cutting techniques. I used to do this with my students and it really helped them. > > Good luck! > > > > Andi G. > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rachel M Gonzalez [rachel@gbi-inc.com] > > Sent: Monday, April 20, 2015 9:07 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] prevent wrinkles when cutting > > > > Hi > > > > Thursday was the first time I ever used a microtome.... I move to a lab > > that does not have someone dedicated to cutting. I already miss her. > > > > I have no problems getting ribbons of 10-30 sections long but the pieces > > are half the size of the original block. I am guessing they are wrinkling. > > What am I doing wrong? > > > > Thanks > > Rachel > > Senior Scientist.... > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ????????????????? ???????? ? ???????????????? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rachel <@t> gbi-inc.com Mon Apr 20 16:04:31 2015 From: rachel <@t> gbi-inc.com (Rachel M Gonzalez) Date: Mon Apr 20 16:04:35 2015 Subject: [Histonet] prevent wrinkles when cutting In-Reply-To: References: <55353209.4020104@eae.csic.es> Message-ID: Hi The long ribbons are for R&D and QA purposes. Often we test under multiple conditions 1-4 with multiple antibodies to the same tissue or multiple tissue to optimize reagents. It quickly adds up. Rachel On Mon, Apr 20, 2015 at 3:28 PM, Joelle Weaver wrote: > Why are you cutting such a long ribbon? You usually only a need a series > of 3-4 sections even for ribbon cutting. Might be easier to control if you > don't try to move such a long ribbon to the waterbath. Drag the shorter > ribbon towards you on the waterbath. Make sure the water is not too cool. > Face the block to full face but superficial, chill on ice for some > moisture, take sections while the block is still very cold. Use a slow, > steady, smooth stroke if doing manual cutting. Make sure your embedding > works well for the way you orient the block in the holder. Angles work well > for many tissues that are prone to wrinkling. Its mostly just practice > though. The more you cut, the easier it becomes and usually the better you > get at it. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > Date: Mon, 20 Apr 2015 19:06:17 +0200 > > From: j.benavides@eae.csic.es > > To: histonet@lists.utsouthwestern.edu > > Subject: Re: FW: [Histonet] prevent wrinkles when cutting > > > > Hi there, > > > > I?m curious about the soaking thing. We have never done it in our lab. > > Which is the purpose to do it? > > > > Than, after facing the blocks, we chill them in a cold plate so, if > > wanting to do the soaking , when should we? I guess before placing them > > on the cold plate, but that may cause a bit of ice formation? > > > > Thanks a lot for your help > > > > Julio > > > > > > On 20/04/2015 18:40, Grantham, Andrea L - (algranth) wrote: > > > Rachel, > > > First off, are you chilling and soaking the blocks after you face them? > > > Do that and see if there is a difference. > > > Don't try to get many sections to your ribbons. Shoot for a smaller > ribbon (5-6) sections that are good. Cut slowly but consistently. > > > What microtome are you using? Are you using disposable blades and are > they sharp? Don't expect them to cut well if you use the same blade to face > the blocks. If you aren't using disposables, get some! They will make your > life easier. > > > You might try to find a histotech at a local hospital lab who might be > able to give you a hands-on lesson. > > > Don't despair! We all sat down at our microtomes those first times and > suffered trying to get perfect sections. It takes practice. You might make > some blank blocks or blocks with tissue you can spare to practice your > cutting techniques. I used to do this with my students and it really helped > them. > > > Good luck! > > > > > > Andi G. > > > ________________________________________ > > > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of Rachel M Gonzalez > [rachel@gbi-inc.com] > > > Sent: Monday, April 20, 2015 9:07 AM > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] prevent wrinkles when cutting > > > > > > Hi > > > > > > Thursday was the first time I ever used a microtome.... I move to a lab > > > that does not have someone dedicated to cutting. I already miss her. > > > > > > I have no problems getting ribbons of 10-30 sections long but the > pieces > > > are half the size of the original block. I am guessing they are > wrinkling. > > > What am I doing wrong? > > > > > > Thanks > > > Rachel > > > Senior Scientist.... > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b.maryottash <@t> gmail.com Mon Apr 20 16:27:52 2015 From: b.maryottash <@t> gmail.com (Bridget Maryott) Date: Mon Apr 20 16:27:55 2015 Subject: [Histonet] Arizona Society for Histotechnology 2015 Spring Meeting Message-ID: *Arizona Society for Histotechnology Spring Quarterly Meeting* *Saturday, May 9th* Sonora Quest Laboratories/Laboratory Sciences of Arizona 1255 W. Washington St. | Tempe, AZ 85281 8:00-11:30 *Anatomic Pathology and Quality Process Improvement* William DeSalvo Lunch and Social 11:30-1:00 1:00-4:30 *Are You A Control Freak?? Do You Have Control Of Your Pre-Analytics?*? Robert Lott Tanya Ewing-Finchem Please join us for a fun and educational day; continental breakfast and lunch will be provided. *To sign up, please go to: * *www.SignUpGenius.com/go/10C044FACAB23A1FE3-ashspring* -- *-Bridget Maryott, HT (ASCP)* * VP Arizona Society for Histotechnology* From liz <@t> premierlab.com Mon Apr 20 16:59:58 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Apr 20 17:00:07 2015 Subject: [Histonet] prevent wrinkles when cutting In-Reply-To: References: <55353209.4020104@eae.csic.es> Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D8B31@SBS2K8.premierlab.local> Rachel We also run multiple antibodies under multiple parameters and there is no need or reason to cut in one ribbon that many sections. You will still be able collect somewhat serial or the sections that you need with multiple ribbons. You are only able to fit on the waterbath in one ribbon (depending upon the size base mold that you used ) anywhere from 6 to 15 sections. You can place ribbons side by side on the waterbath but for me personally I find it?s a bit more difficult to pick the sections up once you have placed multiple long ribbons on the waterbath. I prefer working with one ribbon at a time, two tops when collecting multiple sections, but that?s just me. We work with animal tissue and tissue that may not have been processed in our lab so we do soak all of the blocks. The other thing you need to consider is that when we section a paraffin block the section thickness is not exactly consistent, we are essentially cutting a wedge as the block warms the sections becomes thicker, so if we can control the number of sections we collect in one ribbon, place the block back on ice before we collect the next ribbon, we can then maintain section thickness better. I would think that as you section 30 +sections in one ribbon the block is warming up and the sections are becoming thicker. Section thickness is extremely important when comparing IHC staining results, staining intensity will change in a 1 micron difference in section thickness. Just my two cents - and I do have images and staining intensity calculations with respects to section thickness. This is something that we have looked at recently. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rachel M Gonzalez Sent: Monday, April 20, 2015 3:05 PM To: Joelle Weaver Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] prevent wrinkles when cutting Hi The long ribbons are for R&D and QA purposes. Often we test under multiple conditions 1-4 with multiple antibodies to the same tissue or multiple tissue to optimize reagents. It quickly adds up. Rachel On Mon, Apr 20, 2015 at 3:28 PM, Joelle Weaver wrote: > Why are you cutting such a long ribbon? You usually only a need a > series of 3-4 sections even for ribbon cutting. Might be easier to > control if you don't try to move such a long ribbon to the waterbath. > Drag the shorter ribbon towards you on the waterbath. Make sure the water is not too cool. > Face the block to full face but superficial, chill on ice for some > moisture, take sections while the block is still very cold. Use a > slow, steady, smooth stroke if doing manual cutting. Make sure your > embedding works well for the way you orient the block in the holder. > Angles work well for many tissues that are prone to wrinkling. Its > mostly just practice though. The more you cut, the easier it becomes > and usually the better you get at it. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > Date: Mon, 20 Apr 2015 19:06:17 +0200 > > From: j.benavides@eae.csic.es > > To: histonet@lists.utsouthwestern.edu > > Subject: Re: FW: [Histonet] prevent wrinkles when cutting > > > > Hi there, > > > > I?m curious about the soaking thing. We have never done it in our lab. > > Which is the purpose to do it? > > > > Than, after facing the blocks, we chill them in a cold plate so, if > > wanting to do the soaking , when should we? I guess before placing > > them on the cold plate, but that may cause a bit of ice formation? > > > > Thanks a lot for your help > > > > Julio > > > > > > On 20/04/2015 18:40, Grantham, Andrea L - (algranth) wrote: > > > Rachel, > > > First off, are you chilling and soaking the blocks after you face them? > > > Do that and see if there is a difference. > > > Don't try to get many sections to your ribbons. Shoot for a > > > smaller > ribbon (5-6) sections that are good. Cut slowly but consistently. > > > What microtome are you using? Are you using disposable blades and > > > are > they sharp? Don't expect them to cut well if you use the same blade to > face the blocks. If you aren't using disposables, get some! They will > make your life easier. > > > You might try to find a histotech at a local hospital lab who > > > might be > able to give you a hands-on lesson. > > > Don't despair! We all sat down at our microtomes those first times > > > and > suffered trying to get perfect sections. It takes practice. You might > make some blank blocks or blocks with tissue you can spare to practice > your cutting techniques. I used to do this with my students and it > really helped them. > > > Good luck! > > > > > > Andi G. > > > ________________________________________ > > > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of Rachel M > Gonzalez [rachel@gbi-inc.com] > > > Sent: Monday, April 20, 2015 9:07 AM > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] prevent wrinkles when cutting > > > > > > Hi > > > > > > Thursday was the first time I ever used a microtome.... I move to > > > a lab that does not have someone dedicated to cutting. I already miss her. > > > > > > I have no problems getting ribbons of 10-30 sections long but the > pieces > > > are half the size of the original block. I am guessing they are > wrinkling. > > > What am I doing wrong? > > > > > > Thanks > > > Rachel > > > Senior Scientist.... > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf <@t> gmail.com Mon Apr 20 17:50:15 2015 From: garreyf <@t> gmail.com (Garrey Faller) Date: Mon Apr 20 17:50:19 2015 Subject: [Histonet] (no subject) In-Reply-To: <1039994919.6475395.1429492006874.JavaMail.zimbra@comcast.net> References: <000f425a.0a664de4196a9639@gmail.com> <1039994919.6475395.1429492006874.JavaMail.zimbra@comcast.net> Message-ID: Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, wrote: > I asked about this in a different vein months ago. Has anyone shown a > strawberry or ground meat or slim jim or orange peel as a bacteria/fungus > control used for diagnostics to an inspector inspecting the lab and was > there any comment from the inspector either positive or negative. Never > heard back anything. > Ray, Lake Forest Park, WA > > ----- Original Message ----- > > From: tjfinney2010@gmail.com > To: histonet@lists.utsouthwestern.edu > Sent: Sunday, April 19, 2015 5:24:53 PM > Subject: [Histonet] (no subject) > > GMS controls > >From my understanding we can't use non human controls on patients. I > could be wrong, but you may want to look into it. > > Happy Connecting. Sent from my Sprint Phone. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akoug <@t> hotmail.com Tue Apr 21 00:54:48 2015 From: akoug <@t> hotmail.com (=?utf-8?B?QW5kcsOpIEs=?=) Date: Tue Apr 21 00:54:56 2015 Subject: [Histonet] Verhoeff's hematoxylin Message-ID: Hi 1) Our college is trying to save on reagents. Most textbooks say that Verhoeff's working solution is stable only for a few hours. Someone told me that he used to keep the same solution for up to a month. Has any of you tried to reuse Verhoeff's working solution? If so, how long did you keep it? 2) When preparing stock solution B (10% ferric chloride), do you use the anhydrous or the hexahydrate crystals ? We've always used 10g of hexahydrate for 100ml of water and it works fine. But I just realized that maybe we've been doing it wrong for years?!? 3) About stock solution A : Someone told me that older solutions work better than fresh ones. Apparently, solutions that are one year old are the best. What's your opinion on that? Andre Kougioumoutzakis College de Rosemont Envoy? de mon iPhone From s.shah <@t> garvan.org.au Tue Apr 21 04:28:07 2015 From: s.shah <@t> garvan.org.au (Shruti Shah) Date: Tue Apr 21 04:29:51 2015 Subject: [Histonet] (no subject) In-Reply-To: References: <000f425a.0a664de4196a9639@gmail.com> <1039994919.6475395.1429492006874.JavaMail.zimbra@comcast.net>, Message-ID: Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor. But I am facing problem with bone marrow shrinkage. If any one have idea for decalcification timing and solution can resolved this problem and keep bone marrow intact with bone. Thank you in advance. Regards, Shruti Sent from my iPhone > On 21 Apr 2015, at 8:51 am, "Garrey Faller" wrote: > > Here is the CAP checklist requirement: > ANP.21450 > All histochemical stains are of adequate quality, and daily controls are > demonstrated on each day of use for the tissue components or organism for > which they were designed. > > Ray...you should call the CAP and ask for guidance on this. > My interpretation of this requirement is that it should be OK to use a > fungus from an orange peel. An orange peel fungus should have the same > staining characteristics as a candida or aspergillus etc. Similarly a > bacteria is a bacteria. If you can produce a control that has both gram > positives and negatives, it should be OK. But, don't quote me on this. > > Call the CAP for a definitive answer. I am interested in their response. > Garrey > >> On Sun, Apr 19, 2015 at 9:06 PM, wrote: >> >> I asked about this in a different vein months ago. Has anyone shown a >> strawberry or ground meat or slim jim or orange peel as a bacteria/fungus >> control used for diagnostics to an inspector inspecting the lab and was >> there any comment from the inspector either positive or negative. Never >> heard back anything. >> Ray, Lake Forest Park, WA >> >> ----- Original Message ----- >> >> From: tjfinney2010@gmail.com >> To: histonet@lists.utsouthwestern.edu >> Sent: Sunday, April 19, 2015 5:24:53 PM >> Subject: [Histonet] (no subject) >> >> GMS controls >>> From my understanding we can't use non human controls on patients. I >> could be wrong, but you may want to look into it. >> >> Happy Connecting. Sent from my Sprint Phone. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE Please consider the environment before printing this email. This message and any attachments are intended for the addressee named and may contain legally privileged/confidential/copyright information. If you are not the intended recipient, you should not read, use, disclose, copy or distribute this communication. If you have received this message in error please notify us at once by return email and then delete both messages. We accept no liability for the distribution of viruses or similar in electronic communications. This notice should not be removed. From jshelley <@t> sanfordburnham.org Tue Apr 21 07:28:06 2015 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Tue Apr 21 07:28:13 2015 Subject: [Histonet] Florida Society for Histotechnology Meeting Message-ID: Good Morning Histonetters! Did you know or may be even care to know that we have only 3 days left to guarantee a hotel room for our 2015 Spring FSH Meeting. Final reservations should be made no later than April 23rd, 2015 to ensure rate and availability. After that date you will have to hope that room prices will not go up. Act now to save money and possible aggravation. Looking forward to seeing you at our meeting! By the way all are welcome from near or far! Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402&hotelID=6579 Need to book no later than 4-23-15 Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155&lbrd=1&rtypeid=380141 Have a great day! Kind Regards! John J Shelley 2014-2016 FSH President From koellingr <@t> comcast.net Tue Apr 21 08:49:21 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Apr 21 08:49:51 2015 Subject: [Histonet] controls to lengthy off topic In-Reply-To: References: <000f425a.0a664de4196a9639@gmail.com> <1039994919.6475395.1429492006874.JavaMail.zimbra@comcast.net> Message-ID: <2017966350.7376432.1429624161902.JavaMail.zimbra@comcast.net> Hello Garrey, Curious myself, CAP contact info seems to be greyed out on website unless I officially log in and for now my concerns are with the Washington State Science and Engineering Fair for K-12 and golf game. ? (1) There are at least two phrases in the ANP.21450 which could be parsed out similar to the now famous "it depends on what the definition of is is". (2) Fortunate, I had micro groups around who could provide me with species specific Candida or Aspergillus or species and morphological identifiable gram positive or gram negative organisms so when I built the controls with fresh human tissue, as has been?described several times on Histonet by others, I knew exactly what I was looking at. (3) It appears there may be tens to hundreds of thousands of "molds" and what is growing in orange peels or strawberries or cream cheese or bacteria in slim jims would be a total mystery but maybe that is OK. Yet, human pathogen or not? rare or common? stains appropriately or not according to what it REALLY is? ? I'm not saying the controls are wrong; they might be perfectly fine.? I'm just curious if anyone being inspected ever put a stained section of a slim jim on scope in front of a Pathologist from the inspecting agency and what was the reaction if any. ? Ray in Lake Forest Park, WA ----- Original Message ----- From: "Garrey Faller" To: koellingr@comcast.net Cc: tjfinney2010@gmail.com, histonet@lists.utsouthwestern.edu Sent: Monday, April 20, 2015 3:50:15 PM Subject: Re: [Histonet] (no subject) Here is the CAP checklist requirement: ANP.21450 All ?histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc.? Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this.? Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, < koellingr@comcast.net > wrote: I asked about this in a different vein months ago.? Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from?the inspector?either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA ----- Original Message ----- From: tjfinney2010@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls >From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting.? Sent from my Sprint Phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Apr 21 09:11:38 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Apr 21 09:11:50 2015 Subject: [Histonet] Visual alert for processors Message-ID: <93063ECA-FE1C-403A-B11D-1B5918650D6A@geisinger.edu> Does anyone know of a way to generate a visual alert (hearing impaired device?) to notify us when our processors finish? They are going to be elsewhere in the building and we need to know when they finish. Baby monitor is out of the question due to "privacy issues." Sent from my iPhone IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From taylor <@t> prometheushealthcare.com Tue Apr 21 11:01:25 2015 From: taylor <@t> prometheushealthcare.com (Taylor Rinaldi) Date: Tue Apr 21 10:55:41 2015 Subject: [Histonet] IHC technician needed in Modesto, CA. Message-ID: <024d01d07c4c$6c619e80$4524db80$@prometheushealthcare.com> A growing anatomic pathology laboratory in Modesto, CA is looking to hire a IHC technician for their Histology department. ASCP certification preferred. This is a permanent, full time opportunity offering relocation assistance. Please reach out to me for immediate consideration. Thanks so much! Taylor Rinaldi Nationwide Laboratory Recruiter Prometheus Healthcare Office (301) 693-9057 Taylor@prometheushealthcare.com From jshin06 <@t> gmail.com Tue Apr 21 11:20:24 2015 From: jshin06 <@t> gmail.com (June Shin) Date: Tue Apr 21 11:20:32 2015 Subject: [Histonet] Fwd: FW: IQMH Symposium, Toronto, ON CANADA June 4/5, 2015 In-Reply-To: References: Message-ID: *From:* June Shin *Sent:* April-21-15 11:53 AM *To:* 'histonet@lists.utsouthwestern.edu' *Subject:* IQMH Symposium, Toronto, ON CANADA June 4/5, 2015 *The 2015 IQMH Symposium* The demands of personalized medicine are upon us, and it is critical that patient tissue specimens are confidently handled with the utmost of care. In pathology, part of protecting patient safety involves having a reliable mechanism to control and track patient tissue specimens. Exact knowledge of ischemia time, fixation and processing of positively-identified patient specimens are vital in identifying whether a human tissue specimen can be utilized for subsequent biomarkers, molecular/genetic studies. This symposium is of direct interest to those professionally engaged in healthcare and medical research, and who are concerned with the handling of human tissue; in particular, pathology laboratories, tissue banks and research units. There will be interactive and plenary sessions, and an opportunity for networking and industry exhibits. *Theme:* Best practices in pathology: knowing exactly how you handle each human tissue specimen you process *Date:* June 4-5, 2015 *Venue:* Hilton Garden Inn Toronto Airport 3311 Caroga Drive Mississauga, ON L4V 1A3 * Registration* *Registration is online only.* Fee: $350 + HST (no refunds or cancellations) *Register for this event:* https://iqmh.org/Shop/Product-Viewer/slug/IQMH-Symposium-2015 ------------------------------ This email has been scanned for email related threats and delivered safely by Mimecast. For more information please visit http://www.mimecast.com ------------------------------ From abtdhu <@t> gmail.com Tue Apr 21 12:07:32 2015 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Tue Apr 21 12:07:35 2015 Subject: [Histonet] mice tibia histology Message-ID: Your decal time should be according to your mice age. Four weeks old mouse normally need four changes of EDTA in two weeks time. I think your process time is a bit too long. If your four weeks old mouse tibia, 13 hours from 70% ethanol to last infiltration paraffin should be fine. Your shrinkage is because 1. temperature too high?; 2. too long in xylene? hope this help. Dorothy Hu Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor. But I am facing problem with bone marrow shrinkage. If any one have idea for decalcification timing and solution can resolved this problem and keep bone marrow intact with bone. Thank you in advance. Regards, Shruti From Royl1 <@t> LabCorp.com Tue Apr 21 12:40:43 2015 From: Royl1 <@t> LabCorp.com (Roy, Lisa) Date: Tue Apr 21 12:41:07 2015 Subject: [Histonet] Nuclear "Artifact" Message-ID: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear "artifact". It is described as "washed out" or poor to no nuclear detail. Pictures have been uploaded (Nuclear "Artifact"). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. Lisa Roy, HT(ASCP) Histology Supervisor LabCorp at St. Vincent Hospital 123 Summer St Worcester, MA (508)363-9420 -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From lblazek <@t> digestivespecialists.com Tue Apr 21 12:48:07 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Apr 21 12:48:13 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39173CBFC624@IBMB7Exchange.digestivespecialists.com> The first place I would look is to what may be happening before they reach me. If it's only one site with an issue, it sounds more like an issue at collection. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa Sent: Tuesday, April 21, 2015 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear "Artifact" Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear "artifact". It is described as "washed out" or poor to no nuclear detail. Pictures have been uploaded (Nuclear "Artifact"). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. Lisa Roy, HT(ASCP) Histology Supervisor LabCorp at St. Vincent Hospital 123 Summer St Worcester, MA (508)363-9420 -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Apr 21 12:53:21 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Apr 21 12:53:26 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632616787C90@LRGHEXVS1.practice.lrgh.org> I had this issue a couple of years back. Found out that there was a delay of over an hour from the time the specimen was collected to the time it was being put into fixative. Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa Sent: Tuesday, April 21, 2015 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear "Artifact" Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear "artifact". It is described as "washed out" or poor to no nuclear detail. Pictures have been uploaded (Nuclear "Artifact"). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. Lisa Roy, HT(ASCP) Histology Supervisor LabCorp at St. Vincent Hospital 123 Summer St Worcester, MA (508)363-9420 -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From joelleweaver <@t> hotmail.com Tue Apr 21 14:08:09 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Tue Apr 21 14:08:13 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39173CBFC624@IBMB7Exchange.digestivespecialists.com> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com>, <5A2BD13465E061429D6455C8D6B40E39173CBFC624@IBMB7Exchange.digestivespecialists.com> Message-ID: When I had this occur recently and sporadically, it was a collection issue. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lblazek@digestivespecialists.com > To: Royl1@LabCorp.com; histonet@lists.utsouthwestern.edu > Date: Tue, 21 Apr 2015 13:48:07 -0400 > CC: > Subject: [Histonet] RE: Nuclear "Artifact" > > The first place I would look is to what may be happening before they reach me. If it's only one site with an issue, it sounds more like an issue at collection. > Linda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa > Sent: Tuesday, April 21, 2015 1:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Nuclear "Artifact" > > Hi HistoNetters: > I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear "artifact". It is described as "washed out" or poor to no nuclear detail. Pictures have been uploaded (Nuclear "Artifact"). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. > We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. > > Lisa Roy, HT(ASCP) > Histology Supervisor > LabCorp at St. Vincent Hospital > 123 Summer St > Worcester, MA > (508)363-9420 > > -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Tue Apr 21 14:12:14 2015 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Apr 21 14:12:20 2015 Subject: [Histonet] RE: (no subject) Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D55A389@D1PWPEXMBX05.mdanderson.edu> -I think you can use the other tissue controls. Even CDC (Hansen's Disease Center) will send out mycobacterium leprae controls free of charge, and I think they are armadillo. The best control I have ever used for mast cells is canine mast cell tumors. I request them from Vet Schools regularly. Also think of antibodies, aren't most antibodies animal? The separation of the specimens I think is during processing. They should be processed separately from routine human tissues when they are being used for clinical human tests. I have heard of them running on the same processor, just on a different run when the solutions have been changed. Toysha ----------------------------- Message: 10 Date: Mon, 20 Apr 2015 18:50:15 -0400 From: Garrey Faller Subject: Re: [Histonet] (no subject) To: koellingr@comcast.net Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, wrote: > I asked about this in a different vein months ago. Has anyone shown a > strawberry or ground meat or slim jim or orange peel as a bacteria/fungus > control used for diagnostics to an inspector inspecting the lab and was > there any comment from the inspector either positive or negative. Never > heard back anything. > Ray, Lake Forest Park, WA > > ----- Original Message ----- > > From: tjfinney2010@gmail.com > To: histonet@lists.utsouthwestern.edu > Sent: Sunday, April 19, 2015 5:24:53 PM > Subject: [Histonet] (no subject) > > GMS controls > >From my understanding we can't use non human controls on patients. I > could be wrong, but you may want to look into it. > > Happy Connecting. Sent from my Sprint Phone. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Sent from my iPhone > On 21 Apr 2015, at 8:51 am, "Garrey Faller" wrote: > > Here is the CAP checklist requirement: > ANP.21450 > All histochemical stains are of adequate quality, and daily controls are > demonstrated on each day of use for the tissue components or organism for > which they were designed. > > Ray...you should call the CAP and ask for guidance on this. > My interpretation of this requirement is that it should be OK to use a > fungus from an orange peel. An orange peel fungus should have the same > staining characteristics as a candida or aspergillus etc. Similarly a > bacteria is a bacteria. If you can produce a control that has both gram > positives and negatives, it should be OK. But, don't quote me on this. > > Call the CAP for a definitive answer. I am interested in their response. > Garrey > >> On Sun, Apr 19, 2015 at 9:06 PM, wrote: >> >> I asked about this in a different vein months ago. Has anyone shown a >> strawberry or ground meat or slim jim or orange peel as a bacteria/fungus >> control used for diagnostics to an inspector inspecting the lab and was >> there any comment from the inspector either positive or negative. Never >> heard back anything. >> Ray, Lake Forest Park, WA >> >> ----- Original Message ----- >> >> From: tjfinney2010@gmail.com >> To: histonet@lists.utsouthwestern.edu >> Sent: Sunday, April 19, 2015 5:24:53 PM >> Subject: [Histonet] (no subject) >> >> GMS controls >>> From my understanding we can't use non human controls on patients. I >> could be wrong, but you may want to look into it. >> ------------------------------ Message: 14 Date: Tue, 21 Apr 2015 13:49:21 +0000 (UTC) From: koellingr@comcast.net Subject: Re: [Histonet] controls to lengthy off topic To: Garrey Faller Cc: histonet@lists.utsouthwestern.edu Message-ID: <2017966350.7376432.1429624161902.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 Hello Garrey, Curious myself, CAP contact info seems to be greyed out on website unless I officially log in and for now my concerns are with the Washington State Science and Engineering Fair for K-12 and golf game. ?? (1) There are at least two phrases in the ANP.21450 which could be parsed out similar to the now famous "it depends on what the definition of is is". (2) Fortunate, I had micro groups around who could provide me with species specific Candida or Aspergillus or species and morphological identifiable gram positive or gram negative organisms so when I built the controls with fresh human tissue, as has been??described several times on Histonet by others, I knew exactly what I was looking at. (3) It appears there may be tens to hundreds of thousands of "molds" and what is growing in orange peels or strawberries or cream cheese or bacteria in slim jims would be a total mystery but maybe that is OK. Yet, human pathogen or not? rare or common? stains appropriately or not according to what it REALLY is? ?? I'm not saying the controls are wrong; they might be perfectly fine.?? I'm just curious if anyone being inspected ever put a stained section of a slim jim on scope in front of a Pathologist from the inspecting agency and what was the reaction if any. ?? Ray in Lake Forest Park, WA ----- Original Message ----- From: "Garrey Faller" To: koellingr@comcast.net Cc: tjfinney2010@gmail.com, histonet@lists.utsouthwestern.edu Sent: Monday, April 20, 2015 3:50:15 PM Subject: Re: [Histonet] (no subject) Here is the CAP checklist requirement: ANP.21450 All ??histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc.?? Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this.?? Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, < koellingr@comcast.net > wrote: I asked about this in a different vein months ago.?? Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from??the inspector??either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA ----- Original Message ----- From: tjfinney2010@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls >From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting.?? Sent from my Sprint Phone. _______________________________________________ From craigak12 <@t> gmail.com Tue Apr 21 15:16:02 2015 From: craigak12 <@t> gmail.com (Jb) Date: Tue Apr 21 15:16:10 2015 Subject: [Histonet] IHC/SS QA/QC Sheets: Message-ID: <5AA8C81C-7D40-4776-B629-F29B28748222@gmail.com> Can someone help guide me on the right direction regarding how to organize daily QC sheets. Currently we have one per case (this is time consuming and I'm on overload of papers). Does anyone have a good solution and are you willing to share? Thank you for your help, Craig Sent from my iPhone From tony.henwood <@t> health.nsw.gov.au Tue Apr 21 15:22:44 2015 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Apr 21 15:23:00 2015 Subject: [Histonet] Verhoeff's hematoxylin In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EA31C@xmdb04.nch.kids> Suggested Answers below 1. several hours is all you will get out of the solution I am surprised there is a method that gives you a month shelf life unless it is a different method eg orcein 2. If it works keep using it 3. Allowing the alcoholic Hx to ripen for at least a few days will make it easier to differentiate the elastic fibres. Regards, Tony ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Andr? K [akoug@hotmail.com] Sent: Tuesday, 21 April 2015 3:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Verhoeff's hematoxylin Hi 1) Our college is trying to save on reagents. Most textbooks say that Verhoeff's working solution is stable only for a few hours. Someone told me that he used to keep the same solution for up to a month. Has any of you tried to reuse Verhoeff's working solution? If so, how long did you keep it? L 2) When preparing stock solution B (10% ferric chloride), do you use the anhydrous or the hexahydrate crystals ? We've always used 10g of hexahydrate for 100ml of water and it works fine. But I just realized that maybe we've been doing it wrong for years?!? 3) About stock solution A : Someone told me that older solutions work better than fresh ones. Apparently, solutions that are one year old are the best. What's your opinion on that? Andre Kougioumoutzakis College de Rosemont Envoy? de mon iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From robinsoc <@t> mercyhealth.com Tue Apr 21 15:33:44 2015 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Tue Apr 21 15:33:50 2015 Subject: [Histonet] RE: (no subject) In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC881D55A389@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC881D55A389@D1PWPEXMBX05.mdanderson.edu> Message-ID: <4EE642D353925D4D96CB95E12427DBAE1F0790FA@NODCMSTMBX06.no.trinity-health.org> A few years ago we got cited for not having the fungal control be in tissue. The citation was from a HQIP survey we participated in from CAP. We were using a cultured fungal specimen in a cell button at that time. Since then I have collected and shared a number of cases we have seen with fungal infections in feet. Just my 2 cents........ Cindi Robinson, HT(ASCP) Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Mayer,Toysha N [TNMayer@mdanderson.org] Sent: Tuesday, April 21, 2015 2:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: (no subject) -I think you can use the other tissue controls. Even CDC (Hansen's Disease Center) will send out mycobacterium leprae controls free of charge, and I think they are armadillo. The best control I have ever used for mast cells is canine mast cell tumors. I request them from Vet Schools regularly. Also think of antibodies, aren't most antibodies animal? The separation of the specimens I think is during processing. They should be processed separately from routine human tissues when they are being used for clinical human tests. I have heard of them running on the same processor, just on a different run when the solutions have been changed. Toysha ----------------------------- Message: 10 Date: Mon, 20 Apr 2015 18:50:15 -0400 From: Garrey Faller Subject: Re: [Histonet] (no subject) To: koellingr@comcast.net Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, wrote: > I asked about this in a different vein months ago. Has anyone shown a > strawberry or ground meat or slim jim or orange peel as a bacteria/fungus > control used for diagnostics to an inspector inspecting the lab and was > there any comment from the inspector either positive or negative. Never > heard back anything. > Ray, Lake Forest Park, WA > > ----- Original Message ----- > > From: tjfinney2010@gmail.com > To: histonet@lists.utsouthwestern.edu > Sent: Sunday, April 19, 2015 5:24:53 PM > Subject: [Histonet] (no subject) > > GMS controls > >From my understanding we can't use non human controls on patients. I > could be wrong, but you may want to look into it. > > Happy Connecting. Sent from my Sprint Phone. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Sent from my iPhone > On 21 Apr 2015, at 8:51 am, "Garrey Faller" wrote: > > Here is the CAP checklist requirement: > ANP.21450 > All histochemical stains are of adequate quality, and daily controls are > demonstrated on each day of use for the tissue components or organism for > which they were designed. > > Ray...you should call the CAP and ask for guidance on this. > My interpretation of this requirement is that it should be OK to use a > fungus from an orange peel. An orange peel fungus should have the same > staining characteristics as a candida or aspergillus etc. Similarly a > bacteria is a bacteria. If you can produce a control that has both gram > positives and negatives, it should be OK. But, don't quote me on this. > > Call the CAP for a definitive answer. I am interested in their response. > Garrey > >> On Sun, Apr 19, 2015 at 9:06 PM, wrote: >> >> I asked about this in a different vein months ago. Has anyone shown a >> strawberry or ground meat or slim jim or orange peel as a bacteria/fungus >> control used for diagnostics to an inspector inspecting the lab and was >> there any comment from the inspector either positive or negative. Never >> heard back anything. >> Ray, Lake Forest Park, WA >> >> ----- Original Message ----- >> >> From: tjfinney2010@gmail.com >> To: histonet@lists.utsouthwestern.edu >> Sent: Sunday, April 19, 2015 5:24:53 PM >> Subject: [Histonet] (no subject) >> >> GMS controls >>> From my understanding we can't use non human controls on patients. I >> could be wrong, but you may want to look into it. >> ------------------------------ Message: 14 Date: Tue, 21 Apr 2015 13:49:21 +0000 (UTC) From: koellingr@comcast.net Subject: Re: [Histonet] controls to lengthy off topic To: Garrey Faller Cc: histonet@lists.utsouthwestern.edu Message-ID: <2017966350.7376432.1429624161902.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 Hello Garrey, Curious myself, CAP contact info seems to be greyed out on website unless I officially log in and for now my concerns are with the Washington State Science and Engineering Fair for K-12 and golf game. ?? (1) There are at least two phrases in the ANP.21450 which could be parsed out similar to the now famous "it depends on what the definition of is is". (2) Fortunate, I had micro groups around who could provide me with species specific Candida or Aspergillus or species and morphological identifiable gram positive or gram negative organisms so when I built the controls with fresh human tissue, as has been??described several times on Histonet by others, I knew exactly what I was looking at. (3) It appears there may be tens to hundreds of thousands of "molds" and what is growing in orange peels or strawberries or cream cheese or bacteria in slim jims would be a total mystery but maybe that is OK. Yet, human pathogen or not? rare or common? stains appropriately or not according to what it REALLY is? ?? I'm not saying the controls are wrong; they might be perfectly fine.?? I'm just curious if anyone being inspected ever put a stained section of a slim jim on scope in front of a Pathologist from the inspecting agency and what was the reaction if any. ?? Ray in Lake Forest Park, WA ----- Original Message ----- From: "Garrey Faller" To: koellingr@comcast.net Cc: tjfinney2010@gmail.com, histonet@lists.utsouthwestern.edu Sent: Monday, April 20, 2015 3:50:15 PM Subject: Re: [Histonet] (no subject) Here is the CAP checklist requirement: ANP.21450 All ??histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc.?? Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this.?? Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, < koellingr@comcast.net > wrote: I asked about this in a different vein months ago.?? Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from??the inspector??either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA ----- Original Message ----- From: tjfinney2010@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls >From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting.?? Sent from my Sprint Phone. _______________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From s.shah <@t> garvan.org.au Tue Apr 21 16:01:54 2015 From: s.shah <@t> garvan.org.au (Shruti Shah) Date: Tue Apr 21 16:03:24 2015 Subject: [Histonet] Fwd: mouse tibia histology In-Reply-To: References: <000f425a.0a664de4196a9639@gmail.com> <1039994919.6475395.1429492006874.JavaMail.zimbra@comcast.net>, , Message-ID: <34DA50D2-F429-4137-B2B6-6348CA3F18F2@garvan.org.au> Sent from my iPhone Begin forwarded message: From: Shruti Shah > Date: 21 April 2015 7:28:07 pm AEST To: Garrey Faller > Cc: "histonet@lists.utsouthwestern.edu" > Subject: Re: [Histonet] (no subject) Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor. But I am facing problem with bone marrow shrinkage. If any one have idea for decalcification timing and solution can resolved this problem and keep bone marrow intact with bone. Thank you in advance. Regards, Shruti Sent from my iPhone On 21 Apr 2015, at 8:51 am, "Garrey Faller" > wrote: Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, > wrote: I asked about this in a different vein months ago. Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA ----- Original Message ----- From: tjfinney2010@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls >From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting. Sent from my Sprint Phone. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE Please consider the environment before printing this email. This message and any attachments are intended for the addressee named and may contain legally privileged/confidential/copyright information. If you are not the intended recipient, you should not read, use, disclose, copy or distribute this communication. If you have received this message in error please notify us at once by return email and then delete both messages. We accept no liability for the distribution of viruses or similar in electronic communications. This notice should not be removed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE Please consider the environment before printing this email. This message and any attachments are intended for the addressee named and may contain legally privileged/confidential/copyright information. If you are not the intended recipient, you should not read, use, disclose, copy or distribute this communication. If you have received this message in error please notify us at once by return email and then delete both messages. We accept no liability for the distribution of viruses or similar in electronic communications. This notice should not be removed. From akbitting <@t> geisinger.edu Tue Apr 21 16:21:07 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Apr 21 16:21:50 2015 Subject: [Histonet] autdialer for Peloris Message-ID: <85c197fc3191490190960f4c352615b9@GHSEXMBX4W12V.geisinger.edu> Can anyone recommend a third party auto dialer to connect to Peloris? Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From TNMayer <@t> mdanderson.org Tue Apr 21 17:32:46 2015 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Apr 21 17:33:19 2015 Subject: [Histonet] RE: (no subject) In-Reply-To: <4EE642D353925D4D96CB95E12427DBAE1F0790FA@NODCMSTMBX06.no.trinity-health.org> References: <47E9B2C01DDDD94881EACD2DC44EBC881D55A389@D1PWPEXMBX05.mdanderson.edu> <4EE642D353925D4D96CB95E12427DBAE1F0790FA@NODCMSTMBX06.no.trinity-health.org> Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D55AB35@D1PWPEXMBX05.mdanderson.edu> Cynthia, That is good to know. Then I wonder how the CDC gets away with sending the armadillo controls out for leprae. We used it for FITE stains only, not regular AFB. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -----Original Message----- From: Cynthia Robinson [mailto:robinsoc@mercyhealth.com] Sent: Tuesday, April 21, 2015 3:34 PM To: Mayer,Toysha N; 'histonet@lists.utsouthwestern.edu' Subject: RE: (no subject) A few years ago we got cited for not having the fungal control be in tissue. The citation was from a HQIP survey we participated in from CAP. We were using a cultured fungal specimen in a cell button at that time. Since then I have collected and shared a number of cases we have seen with fungal infections in feet. Just my 2 cents........ Cindi Robinson, HT(ASCP) Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Mayer,Toysha N [TNMayer@mdanderson.org] Sent: Tuesday, April 21, 2015 2:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: (no subject) -I think you can use the other tissue controls. Even CDC (Hansen's Disease Center) will send out mycobacterium leprae controls free of charge, and I think they are armadillo. The best control I have ever used for mast cells is canine mast cell tumors. I request them from Vet Schools regularly. Also think of antibodies, aren't most antibodies animal? The separation of the specimens I think is during processing. They should be processed separately from routine human tissues when they are being used for clinical human tests. I have heard of them running on the same processor, just on a different run when the solutions have been changed. Toysha ----------------------------- Message: 10 Date: Mon, 20 Apr 2015 18:50:15 -0400 From: Garrey Faller Subject: Re: [Histonet] (no subject) To: koellingr@comcast.net Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, wrote: > I asked about this in a different vein months ago. Has anyone shown a > strawberry or ground meat or slim jim or orange peel as a > bacteria/fungus control used for diagnostics to an inspector > inspecting the lab and was there any comment from the inspector either > positive or negative. Never heard back anything. > Ray, Lake Forest Park, WA > > ----- Original Message ----- > > From: tjfinney2010@gmail.com > To: histonet@lists.utsouthwestern.edu > Sent: Sunday, April 19, 2015 5:24:53 PM > Subject: [Histonet] (no subject) > > GMS controls > >From my understanding we can't use non human controls on patients. I > could be wrong, but you may want to look into it. > > Happy Connecting. Sent from my Sprint Phone. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Sent from my iPhone > On 21 Apr 2015, at 8:51 am, "Garrey Faller" wrote: > > Here is the CAP checklist requirement: > ANP.21450 > All histochemical stains are of adequate quality, and daily controls > are demonstrated on each day of use for the tissue components or > organism for which they were designed. > > Ray...you should call the CAP and ask for guidance on this. > My interpretation of this requirement is that it should be OK to use a > fungus from an orange peel. An orange peel fungus should have the same > staining characteristics as a candida or aspergillus etc. Similarly a > bacteria is a bacteria. If you can produce a control that has both > gram positives and negatives, it should be OK. But, don't quote me on this. > > Call the CAP for a definitive answer. I am interested in their response. > Garrey > >> On Sun, Apr 19, 2015 at 9:06 PM, wrote: >> >> I asked about this in a different vein months ago. Has anyone shown >> a strawberry or ground meat or slim jim or orange peel as a >> bacteria/fungus control used for diagnostics to an inspector >> inspecting the lab and was there any comment from the inspector >> either positive or negative. Never heard back anything. >> Ray, Lake Forest Park, WA >> >> ----- Original Message ----- >> >> From: tjfinney2010@gmail.com >> To: histonet@lists.utsouthwestern.edu >> Sent: Sunday, April 19, 2015 5:24:53 PM >> Subject: [Histonet] (no subject) >> >> GMS controls >>> From my understanding we can't use non human controls on patients. I >> could be wrong, but you may want to look into it. >> ------------------------------ Message: 14 Date: Tue, 21 Apr 2015 13:49:21 +0000 (UTC) From: koellingr@comcast.net Subject: Re: [Histonet] controls to lengthy off topic To: Garrey Faller Cc: histonet@lists.utsouthwestern.edu Message-ID: <2017966350.7376432.1429624161902.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 Hello Garrey, Curious myself, CAP contact info seems to be greyed out on website unless I officially log in and for now my concerns are with the Washington State Science and Engineering Fair for K-12 and golf game. ?? (1) There are at least two phrases in the ANP.21450 which could be parsed out similar to the now famous "it depends on what the definition of is is". (2) Fortunate, I had micro groups around who could provide me with species specific Candida or Aspergillus or species and morphological identifiable gram positive or gram negative organisms so when I built the controls with fresh human tissue, as has been??described several times on Histonet by others, I knew exactly what I was looking at. (3) It appears there may be tens to hundreds of thousands of "molds" and what is growing in orange peels or strawberries or cream cheese or bacteria in slim jims would be a total mystery but maybe that is OK. Yet, human pathogen or not? rare or common? stains appropriately or not according to what it REALLY is? ?? I'm not saying the controls are wrong; they might be perfectly fine.?? I'm just curious if anyone being inspected ever put a stained section of a slim jim on scope in front of a Pathologist from the inspecting agency and what was the reaction if any. ?? Ray in Lake Forest Park, WA ----- Original Message ----- From: "Garrey Faller" To: koellingr@comcast.net Cc: tjfinney2010@gmail.com, histonet@lists.utsouthwestern.edu Sent: Monday, April 20, 2015 3:50:15 PM Subject: Re: [Histonet] (no subject) Here is the CAP checklist requirement: ANP.21450 All ??histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc.?? Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this.?? Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, < koellingr@comcast.net > wrote: I asked about this in a different vein months ago.?? Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from??the inspector??either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA ----- Original Message ----- From: tjfinney2010@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls >From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting.?? Sent from my Sprint Phone. _______________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From suetp918 <@t> comcast.net Tue Apr 21 18:55:22 2015 From: suetp918 <@t> comcast.net (Sue) Date: Tue Apr 21 18:55:37 2015 Subject: [Histonet] Nuclear "Artifact" In-Reply-To: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> Message-ID: <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. Susan T. Paturzo TJUH From akbitting <@t> geisinger.edu Tue Apr 21 21:03:39 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Apr 21 21:04:11 2015 Subject: [Histonet] Nuclear "Artifact" In-Reply-To: <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com>, <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> Message-ID: We occasionally see this also. Sent from my iPhone > On Apr 21, 2015, at 7:59 PM, Sue wrote: > > OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. > > Susan T. Paturzo > TJUH > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From mjones <@t> metropath.com Wed Apr 22 09:02:37 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Wed Apr 22 09:02:46 2015 Subject: [Histonet] Nuclear "Artifact" Message-ID: Happy Lab Week!! We worked on our H&E for almost two years. We were using Leica H&E products and after 2 years of struggling, adjusting and analyzing everything in our lab - we switched to the Richard-Allen Scientific products. We were seeing variability between days of staining, tissues right next to each other, nuclear paleness, eosin uniformity instead of differentiated, etc. Switching reagents helped us tremendously - we have more consistent higher quality stains on a daily basis and within tissues. I you?re struggling and have analyzed your processors, etc. to death - maybe try different reagents? (we even measured the tap water that we use on our stainer daily, the pH of reagents every other hour etc. and between 5 experienced histotechs, we couldn?t figure it out) Good luck! :) Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 4/21/15, 5:55 PM, "Sue" wrote: >OMG we are experiencing the same issue. At first it was just GI and now >we are seeing it on prostate. One pathologist said it looks like the >tissue has been cooked. The only issue is we can have two biopsies right >next to one another in the basket one looks good and one looks bad. My >director also thinks it is the processors. I had Thermo out and they >could find nothing. We changed out all the reagents and the biopsies were >fine than two days later we had some bad ones. I know in July Fisher had >a formalin recall associated to the mixture of buffer, water and >formalin. We thought that might be it but it is now almost a year later >and all the bad formalin should be gone. The histotechs say the tissue is >crunchy and they are right. I am running a test tonight of a small needle >biopsy that I made from a colon. I placed it is straight formaldehyde >overnight and am processing it on our biopsy cycle tonight. My director >also wanted us to only put three levels on our Thermo, but he wanted the >middle level to have empty baskets. I stopped that today because I think >the other issue is that the poor biopsies may be on the top level and as >the reagents are used the level changes, and also due to displacement >with the middle level being empty the reagent levels may not reach the >top. We just do not have the manpower to inspect every reagent every day, >we have 6 processor and it would take a tech all day. We actually take a >digital picture when they come out of the processor. I want to check my >problems cases tomorrow. We do not use sponges but the only other like >was the PA who was wrapping the blue paper very tight around the tissue. >I really do not think this is the issue though.. Any other insight would >be greatly appreciated. > >Susan T. Paturzo >TJUH >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Apr 22 09:07:18 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Apr 22 09:07:44 2015 Subject: [Histonet] histology in higher education In-Reply-To: <1886624111.8110643.1429709352958.JavaMail.zimbra@comcast.net> Message-ID: <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net> The following has to do with histology and STEM (science, technology, engineering, math) so if not interested, please ignore.? But I believe it?can have?real meaning to the profession of histology at the NSH, state society and local levels. ? I am elected to the Board of WSSEF (Washington State Science and Engineering Fair) where I am in educational outreach and?also the assistant to the head judge.? We recently had our Washington State Fair with 650 kids, grades 1-12 from all over the state.? And while there was a lot of engineering and robots and computers there were a few projects having to do with medicine, biotechnology, immunology and pathology with some familiar?histology or immunohistochemistry pictures included.? At the end of the fair, we awarded almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 students.? Not only that, our top winners get an all-expenses paid trip to present at the ISEF (Intel International Fair) with 1,700 students competing from all 50 states and 70 countries.? Wherever you are in the US, you have a state fair. ? I would advocate for some of you so interested at the national, state or local levels to promote histology,?by getting?involved as mentors for middle and high school students to science fairs; especially those that could lead to histopathology or other related projects that could lead into Intel affiliated fairs resulting in great benefit to the student and a spread of the word of histology into both the STEM world and general population. ? I've mentored for 15 years.? It can be done.? Molecular histopathology, personalized diagnostics and therapeutics, advances in immunohistochemistry, current controversies about breast biopsy diagnosis, or other disease with newer classifications, PCR and RTPCR in histology, modern-targeted therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image analysis software for you computer geeks and on and on; the list is nearly limitless.? Especially if you are close to or can contact biotech companies or educational institutions to find co-mentors for grades 7-12 there are histology-related science project possibilities in terms of data collection and the scientific method and project presentation are nearly unlimited now. ? Be a mentor for or engage a grade 7-12 student, with the help of another mentor or organization, to think about (histology-related) projects for science fairs leading to a state fair and Intel ISEF.? Can't think of any better way to "promote histology" so would hope those at NSH would take note of this.? And since the ISEF fair receives projects and groups from 70 countries, I hope any outside the US would also think about the same thing. ? Ray Koelling HT, HTL, QIHC, STEM educational outreach advocate Lake Forest Park, WA From rstapf <@t> dallasassocderm.com Wed Apr 22 09:31:12 2015 From: rstapf <@t> dallasassocderm.com (Ross Stapf) Date: Wed Apr 22 09:31:21 2015 Subject: [Histonet] Need Daniel Terry exTBS new company info Message-ID: I was told that Daniel Terry left TBS to work for a private biomed company. I am looking for contact info on this company. He was their main person for the ATP processor. There are too many other Daniel Terry's in that area for google to help. Hopefully someone here uses his new company. Ross M Stapf HT(ASCP) Dallas Associated Dermatologists, P.A. 12700 Park Central Drive Suite B-150 Dallas, TX 75251 Phone # 214-987-3376 x3396 Fax # (214) 420-1441 From wsimons <@t> athensgastro.com Wed Apr 22 09:31:56 2015 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Wed Apr 22 09:32:00 2015 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gaGlzdG9sb2d5IGluIGhpZ2hlciBlZHVjYXRpb24=?= In-Reply-To: <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net> References: <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net> Message-ID: <20150422143156.21929.qmail@server276.com> Good morning Ray and thank you for promoting the field of Histotechnology. While President of the NSH, Vinnie DellaSperanza started a career day function at the annual NSH symposium. It has been very successful and the individuals that contribute to this volunteer effort are usually the same individuals that participate at the state and regional level. Thank you for the idea of state fairs and other avenues for the target age of middle & high school students. I did this myself when my children were in girl scouts , "Odyssey of the Mind" and advance placement opportunities. Wanda K. Simons, HT (ASCP) GSH President www.histosearch.com/gsh/ > -------Original Message------- > From: koellingr@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] histology in higher education > Sent: Apr 22 '15 10:14 > > The following has to do with histology and STEM (science, technology, engineering, math) so if not interested, please ignore.? But I believe it?can have?real meaning to the profession of histology at the NSH, state society and local levels. > ? > I am elected to the Board of WSSEF (Washington State Science and Engineering Fair) where I am in educational outreach and?also the assistant to the head judge.? We recently had our Washington State Fair with 650 kids, grades 1-12 from all over the state.? And while there was a lot of engineering and robots and computers there were a few projects having to do with medicine, biotechnology, immunology and pathology with some familiar?histology or immunohistochemistry pictures included.? At the end of the fair, we awarded almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 students.? Not only that, our top winners get an all-expenses paid trip to present at the ISEF (Intel International Fair) with 1,700 students competing from all 50 states and 70 countries.? Wherever you are in the US, you have a state fair. > ? > I would advocate for some of you so interested at the national, state or local levels to promote histology,?by getting?involved as mentors for middle and high school students to science fairs; especially those that could lead to histopathology or other related projects that could lead into Intel affiliated fairs resulting in great benefit to the student and a spread of the word of histology into both the STEM world and general population. > ? > I've mentored for 15 years.? It can be done.? Molecular histopathology, personalized diagnostics and therapeutics, advances in immunohistochemistry, current controversies about breast biopsy diagnosis, or other disease with newer classifications, PCR and RTPCR in histology, modern-targeted therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image analysis software for you computer geeks and on and on; the list is nearly limitless.? Especially if you are close to or can contact biotech companies or educational institutions to find co-mentors for grades 7-12 there are histology-related science project possibilities in terms of data collection and the scientific method and project presentation are nearly unlimited now. > ? > Be a mentor for or engage a grade 7-12 student, with the help of another mentor or organization, to think about (histology-related) projects for science fairs leading to a state fair and Intel ISEF.? Can't think of any better way to "promote histology" so would hope those at NSH would take note of this.? And since the ISEF fair receives projects and groups from 70 countries, I hope any outside the US would also think about the same thing. > ? > Ray Koelling > HT, HTL, QIHC, STEM educational outreach advocate > Lake Forest Park, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gmarcella <@t> nj-urology.com Wed Apr 22 09:55:19 2015 From: gmarcella <@t> nj-urology.com (Gail Marcella) Date: Wed Apr 22 09:55:25 2015 Subject: [Histonet] NY State regulations Message-ID: <3652170FF5F22544A1280F1DB5D9199416F4284316@NJUE1.nj-urology.com> Hi - I was wondering if anyone knows the regulations regarding the NY State Clinical Laboratory license. I have been a Histotech and have worked in IHC for 20+ years and was required to obtain a NY State Clinical Lab License in 2007. I don't have and associates or bachelor degree and was not required to prior to 2007. I was told on a job interview that if I don't have either of these degrees that I cannot gross any specimens or run IHC. I've never heard this before. Has anyone else ever heard of this??? Thanks - Gail From LSebree <@t> uwhealth.org Wed Apr 22 10:05:16 2015 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Apr 22 10:05:25 2015 Subject: [Histonet] IHC/SS QA/QC Sheets: In-Reply-To: <5AA8C81C-7D40-4776-B629-F29B28748222@gmail.com> References: <5AA8C81C-7D40-4776-B629-F29B28748222@gmail.com> Message-ID: <77DD817201982748BC67D7960F2F76AF131A95@UWHC-MBX13.uwhis.hosp.wisc.edu> Hi Craig, As technologists, we of course QC all slides before they get to the pathologist(s) but that is not documented unless we put a repeat order in the case. Then we add a note saying why a test needs repeating, i.e. positive control did not stain, patient tissue loss, etc. But this is all done electronically, so no paper trail. For general QC, we have gone to having a statement automatically populate to any case reports that include IHC/ISH staining but I'm assuming this could also work for H&Es, Special Stains, etc.; I'm only familiar with the IHC/ISH portion of QCing. Our statement says something along the lines of the controls staining appropriately and as expected. The pathologist that signs out the case is attesting to that statement. This statement has eliminated HUGE amounts of paper and filing. Hope this helps, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Tuesday, April 21, 2015 3:16 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC/SS QA/QC Sheets: Can someone help guide me on the right direction regarding how to organize daily QC sheets. Currently we have one per case (this is time consuming and I'm on overload of papers). Does anyone have a good solution and are you willing to share? Thank you for your help, Craig Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Wed Apr 22 10:16:14 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Apr 22 10:20:51 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> Message-ID: <761E2B5697F795489C8710BCC72141FF36805FC3@ex07.net.ucsf.edu> "One pathologist said it looks like the tissue has been cooked." Which could also be drying artifact after bx, before formalin. "The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors." Same processor but two results? Solving intermittent problems takes time to check variables - time almost no one wants to spend to check out every possibility. But if one variable is the same for both, and the result is different, then most likely it is a different variable causing the problem. I think the other idea suggested of checking the handling of the tissue at the source of the biopsy is more likely to shed some light on this issue. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, April 21, 2015 4:55 PM To: Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear "Artifact" OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. Susan T. Paturzo TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Wed Apr 22 10:39:54 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Apr 22 10:39:59 2015 Subject: [Histonet] histology in higher education In-Reply-To: <20150422143156.21929.qmail@server276.com> References: <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net>, <20150422143156.21929.qmail@server276.com> Message-ID: For a few years I've been involved in a program called "letters to a pre-scientist". The idea is to reach middle schoolers as they are being introduced to the sciences. They have pretty high goals at this time, they want to be doctors and astronauts and engineers but they are just starting to learn about these things. You become a pen pal/mentor of sorts and write letters to a child and they will write back to you. Last year I was writing to a boy in the Chicago area and this year it was a girl in LA. I always write about what I do and how important it is and include pictures of things like brain cells, muscle, fungus, bacteria and pictures of my lab. I always pick up a copy of the NSH coloring book and send it to them and tell them what they need to study to be a histotech and other than a hospital, where they can find a job. Of course we also tell them about other things like our families, pets, vacations, etc. at the same time. It's just a small thing but it plants a seed. Andi G. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of wsimons@athensgastro.com [wsimons@athensgastro.com] Sent: Wednesday, April 22, 2015 7:31 AM To: koellingr@comcast.net; "" Subject: Re: [Histonet] histology in higher education Good morning Ray and thank you for promoting the field of Histotechnology. While President of the NSH, Vinnie DellaSperanza started a career day function at the annual NSH symposium. It has been very successful and the individuals that contribute to this volunteer effort are usually the same individuals that participate at the state and regional level. Thank you for the idea of state fairs and other avenues for the target age of middle & high school students. I did this myself when my children were in girl scouts , "Odyssey of the Mind" and advance placement opportunities. Wanda K. Simons, HT (ASCP) GSH President www.histosearch.com/gsh/ > -------Original Message------- > From: koellingr@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] histology in higher education > Sent: Apr 22 '15 10:14 > > The following has to do with histology and STEM (science, technology, engineering, math) so if not interested, please ignore. But I believe it can have real meaning to the profession of histology at the NSH, state society and local levels. > > I am elected to the Board of WSSEF (Washington State Science and Engineering Fair) where I am in educational outreach and also the assistant to the head judge. We recently had our Washington State Fair with 650 kids, grades 1-12 from all over the state. And while there was a lot of engineering and robots and computers there were a few projects having to do with medicine, biotechnology, immunology and pathology with some familiar histology or immunohistochemistry pictures included. At the end of the fair, we awarded almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 students. Not only that, our top winners get an all-expenses paid trip to present at the ISEF (Intel International Fair) with 1,700 students competing from all 50 states and 70 countries. Wherever you are in the US, you have a state fair. > > I would advocate for some of you so interested at the national, state or local levels to promote histology, by getting involved as mentors for middle and high school students to science fairs; especially those that could lead to histopathology or other related projects that could lead into Intel affiliated fairs resulting in great benefit to the student and a spread of the word of histology into both the STEM world and general population. > > I've mentored for 15 years. It can be done. Molecular histopathology, personalized diagnostics and therapeutics, advances in immunohistochemistry, current controversies about breast biopsy diagnosis, or other disease with newer classifications, PCR and RTPCR in histology, modern-targeted therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image analysis software for you computer geeks and on and on; the list is nearly limitless. Especially if you are close to or can contact biotech companies or educational institutions to find co-mentors for grades 7-12 there are histology-related science project possibilities in terms of data collection and the scientific method and project presentation are nearly unlimited now. > > Be a mentor for or engage a grade 7-12 student, with the help of another mentor or organization, to think about (histology-related) projects for science fairs leading to a state fair and Intel ISEF. Can't think of any better way to "promote histology" so would hope those at NSH would take note of this. And since the ISEF fair receives projects and groups from 70 countries, I hope any outside the US would also think about the same thing. > > Ray Koelling > HT, HTL, QIHC, STEM educational outreach advocate > Lake Forest Park, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.Whitaker <@t> osumc.edu Wed Apr 22 10:45:40 2015 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Wed Apr 22 10:45:53 2015 Subject: [Histonet] histology in higher education In-Reply-To: References: <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net>, <20150422143156.21929.qmail@server276.com> Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E160222D2@EXMBOX-VP05.OSUMC.EDU> Andi, Would you be willing to share the information on how to volunteer with this program? Thanks, Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 11:40 AM To: "" Subject: RE: [Histonet] histology in higher education For a few years I've been involved in a program called "letters to a pre-scientist". The idea is to reach middle schoolers as they are being introduced to the sciences. They have pretty high goals at this time, they want to be doctors and astronauts and engineers but they are just starting to learn about these things. You become a pen pal/mentor of sorts and write letters to a child and they will write back to you. Last year I was writing to a boy in the Chicago area and this year it was a girl in LA. I always write about what I do and how important it is and include pictures of things like brain cells, muscle, fungus, bacteria and pictures of my lab. I always pick up a copy of the NSH coloring book and send it to them and tell them what they need to study to be a histotech and other than a hospital, where they can find a job. Of course we also tell them about other things like our families, pets, vacations, etc. at the same time. It's just a small thing but it plants a seed. Andi G. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of wsimons@athensgastro.com [wsimons@athensgastro.com] Sent: Wednesday, April 22, 2015 7:31 AM To: koellingr@comcast.net; "" Subject: Re: [Histonet] histology in higher education Good morning Ray and thank you for promoting the field of Histotechnology. While President of the NSH, Vinnie DellaSperanza started a career day function at the annual NSH symposium. It has been very successful and the individuals that contribute to this volunteer effort are usually the same individuals that participate at the state and regional level. Thank you for the idea of state fairs and other avenues for the target age of middle & high school students. I did this myself when my children were in girl scouts , "Odyssey of the Mind" and advance placement opportunities. Wanda K. Simons, HT (ASCP) GSH President www.histosearch.com/gsh/ > -------Original Message------- > From: koellingr@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] histology in higher education > Sent: Apr 22 '15 10:14 > > The following has to do with histology and STEM (science, technology, engineering, math) so if not interested, please ignore. But I believe it can have real meaning to the profession of histology at the NSH, state society and local levels. > > I am elected to the Board of WSSEF (Washington State Science and Engineering Fair) where I am in educational outreach and also the assistant to the head judge. We recently had our Washington State Fair with 650 kids, grades 1-12 from all over the state. And while there was a lot of engineering and robots and computers there were a few projects having to do with medicine, biotechnology, immunology and pathology with some familiar histology or immunohistochemistry pictures included. At the end of the fair, we awarded almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 students. Not only that, our top winners get an all-expenses paid trip to present at the ISEF (Intel International Fair) with 1,700 students competing from all 50 states and 70 countries. Wherever you are in the US, you have a state fair. > > I would advocate for some of you so interested at the national, state or local levels to promote histology, by getting involved as mentors for middle and high school students to science fairs; especially those that could lead to histopathology or other related projects that could lead into Intel affiliated fairs resulting in great benefit to the student and a spread of the word of histology into both the STEM world and general population. > > I've mentored for 15 years. It can be done. Molecular histopathology, personalized diagnostics and therapeutics, advances in immunohistochemistry, current controversies about breast biopsy diagnosis, or other disease with newer classifications, PCR and RTPCR in histology, modern-targeted therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image analysis software for you computer geeks and on and on; the list is nearly limitless. Especially if you are close to or can contact biotech companies or educational institutions to find co-mentors for grades 7-12 there are histology-related science project possibilities in terms of data collection and the scientific method and project presentation are nearly unlimited now. > > Be a mentor for or engage a grade 7-12 student, with the help of another mentor or organization, to think about (histology-related) projects for science fairs leading to a state fair and Intel ISEF. Can't think of any better way to "promote histology" so would hope those at NSH would take note of this. And since the ISEF fair receives projects and groups from 70 countries, I hope any outside the US would also think about the same thing. > > Ray Koelling > HT, HTL, QIHC, STEM educational outreach advocate Lake Forest Park, > WA _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKb > mfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=iMDE > ff-OZMAAjL9OtBShZDX-UJn7oIfNUV_V5o3qIXg&s=DVIxzRUB8wWBw-770jBXVTA-wfsm > LPuP6XLZT68y1Ys&e= > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=iMDEff-OZMAAjL9OtBShZDX-UJn7oIfNUV_V5o3qIXg&s=DVIxzRUB8wWBw-770jBXVTA-wfsmLPuP6XLZT68y1Ys&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=iMDEff-OZMAAjL9OtBShZDX-UJn7oIfNUV_V5o3qIXg&s=DVIxzRUB8wWBw-770jBXVTA-wfsmLPuP6XLZT68y1Ys&e= From algranth <@t> email.arizona.edu Wed Apr 22 11:01:28 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Apr 22 11:01:34 2015 Subject: [Histonet] histology in higher education In-Reply-To: <6B106EE8C8AAEF449DEA97921DEC11670E160222D2@EXMBOX-VP05.OSUMC.EDU> References: <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net>, <20150422143156.21929.qmail@server276.com> , <6B106EE8C8AAEF449DEA97921DEC11670E160222D2@EXMBOX-VP05.OSUMC.EDU> Message-ID: Bonnie, and anybody who wants to do this: www.prescientist.org ________________________________________ From: Whitaker, Bonnie [Bonnie.Whitaker@osumc.edu] Sent: Wednesday, April 22, 2015 8:45 AM To: Grantham, Andrea L - (algranth); "" Subject: RE: [Histonet] histology in higher education Andi, Would you be willing to share the information on how to volunteer with this program? Thanks, Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 11:40 AM To: "" Subject: RE: [Histonet] histology in higher education For a few years I've been involved in a program called "letters to a pre-scientist". The idea is to reach middle schoolers as they are being introduced to the sciences. They have pretty high goals at this time, they want to be doctors and astronauts and engineers but they are just starting to learn about these things. You become a pen pal/mentor of sorts and write letters to a child and they will write back to you. Last year I was writing to a boy in the Chicago area and this year it was a girl in LA. I always write about what I do and how important it is and include pictures of things like brain cells, muscle, fungus, bacteria and pictures of my lab. I always pick up a copy of the NSH coloring book and send it to them and tell them what they need to study to be a histotech and other than a hospital, where they can find a job. Of course we also tell them about other things like our families, pets, vacations, etc. at the same time. It's just a small thing but it plants a seed. Andi G. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of wsimons@athensgastro.com [wsimons@athensgastro.com] Sent: Wednesday, April 22, 2015 7:31 AM To: koellingr@comcast.net; "" Subject: Re: [Histonet] histology in higher education Good morning Ray and thank you for promoting the field of Histotechnology. While President of the NSH, Vinnie DellaSperanza started a career day function at the annual NSH symposium. It has been very successful and the individuals that contribute to this volunteer effort are usually the same individuals that participate at the state and regional level. Thank you for the idea of state fairs and other avenues for the target age of middle & high school students. I did this myself when my children were in girl scouts , "Odyssey of the Mind" and advance placement opportunities. Wanda K. Simons, HT (ASCP) GSH President www.histosearch.com/gsh/ > -------Original Message------- > From: koellingr@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] histology in higher education > Sent: Apr 22 '15 10:14 > > The following has to do with histology and STEM (science, technology, engineering, math) so if not interested, please ignore. But I believe it can have real meaning to the profession of histology at the NSH, state society and local levels. > > I am elected to the Board of WSSEF (Washington State Science and Engineering Fair) where I am in educational outreach and also the assistant to the head judge. We recently had our Washington State Fair with 650 kids, grades 1-12 from all over the state. And while there was a lot of engineering and robots and computers there were a few projects having to do with medicine, biotechnology, immunology and pathology with some familiar histology or immunohistochemistry pictures included. At the end of the fair, we awarded almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 students. Not only that, our top winners get an all-expenses paid trip to present at the ISEF (Intel International Fair) with 1,700 students competing from all 50 states and 70 countries. Wherever you are in the US, you have a state fair. > > I would advocate for some of you so interested at the national, state or local levels to promote histology, by getting involved as mentors for middle and high school students to science fairs; especially those that could lead to histopathology or other related projects that could lead into Intel affiliated fairs resulting in great benefit to the student and a spread of the word of histology into both the STEM world and general population. > > I've mentored for 15 years. It can be done. Molecular histopathology, personalized diagnostics and therapeutics, advances in immunohistochemistry, current controversies about breast biopsy diagnosis, or other disease with newer classifications, PCR and RTPCR in histology, modern-targeted therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image analysis software for you computer geeks and on and on; the list is nearly limitless. Especially if you are close to or can contact biotech companies or educational institutions to find co-mentors for grades 7-12 there are histology-related science project possibilities in terms of data collection and the scientific method and project presentation are nearly unlimited now. > > Be a mentor for or engage a grade 7-12 student, with the help of another mentor or organization, to think about (histology-related) projects for science fairs leading to a state fair and Intel ISEF. Can't think of any better way to "promote histology" so would hope those at NSH would take note of this. And since the ISEF fair receives projects and groups from 70 countries, I hope any outside the US would also think about the same thing. > > Ray Koelling > HT, HTL, QIHC, STEM educational outreach advocate Lake Forest Park, > WA _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKb > mfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=iMDE > ff-OZMAAjL9OtBShZDX-UJn7oIfNUV_V5o3qIXg&s=DVIxzRUB8wWBw-770jBXVTA-wfsm > LPuP6XLZT68y1Ys&e= > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=iMDEff-OZMAAjL9OtBShZDX-UJn7oIfNUV_V5o3qIXg&s=DVIxzRUB8wWBw-770jBXVTA-wfsmLPuP6XLZT68y1Ys&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=iMDEff-OZMAAjL9OtBShZDX-UJn7oIfNUV_V5o3qIXg&s=DVIxzRUB8wWBw-770jBXVTA-wfsmLPuP6XLZT68y1Ys&e= From foreightl <@t> gmail.com Wed Apr 22 11:10:53 2015 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Wed Apr 22 11:10:58 2015 Subject: [Histonet] Nuclear "Artifact" In-Reply-To: References: Message-ID: At a previous job, we found (this was specific to prostate biopsies) that one of our clients was taking the prostate biopsies out, lining them up on a dry paper towel, doing the whole procedure, then putting them into the formalin jars. It can be some time between the start and finish of a procedure, so the first couple were looking very dried out (paper towel absorbed most of the moisture) with very pale hematoxylin staining. The pathologist found them almost uninterpretable. When this happened, the first natural area we examined was processing, which in this case turned out not to be the culprit. Collection procedures are not usually done by lab staff, a clinician or staff may have a great idea to make things easier but not know the downstream effects. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Wed, Apr 22, 2015 at 10:02 AM, Michael Ann Jones wrote: > Happy Lab Week!! > We worked on our H&E for almost two years. We were using Leica H&E > products and after 2 years of struggling, adjusting and analyzing > everything in our lab - we switched to the Richard-Allen Scientific > products. We were seeing variability between days of staining, tissues > right next to each other, nuclear paleness, eosin uniformity instead of > differentiated, etc. Switching reagents helped us tremendously - we have > more consistent higher quality stains on a daily basis and within tissues. > > I you?re struggling and have analyzed your processors, etc. to death - > maybe try different reagents? (we even measured the tap water that we use > on our stainer daily, the pH of reagents every other hour etc. and between > 5 experienced histotechs, we couldn?t figure it out) > Good luck! :) > > Michael Ann > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > > > > On 4/21/15, 5:55 PM, "Sue" wrote: > > >OMG we are experiencing the same issue. At first it was just GI and now > >we are seeing it on prostate. One pathologist said it looks like the > >tissue has been cooked. The only issue is we can have two biopsies right > >next to one another in the basket one looks good and one looks bad. My > >director also thinks it is the processors. I had Thermo out and they > >could find nothing. We changed out all the reagents and the biopsies were > >fine than two days later we had some bad ones. I know in July Fisher had > >a formalin recall associated to the mixture of buffer, water and > >formalin. We thought that might be it but it is now almost a year later > >and all the bad formalin should be gone. The histotechs say the tissue is > >crunchy and they are right. I am running a test tonight of a small needle > >biopsy that I made from a colon. I placed it is straight formaldehyde > >overnight and am processing it on our biopsy cycle tonight. My director > >also wanted us to only put three levels on our Thermo, but he wanted the > >middle level to have empty baskets. I stopped that today because I think > >the other issue is that the poor biopsies may be on the top level and as > >the reagents are used the level changes, and also due to displacement > >with the middle level being empty the reagent levels may not reach the > >top. We just do not have the manpower to inspect every reagent every day, > >we have 6 processor and it would take a tech all day. We actually take a > >digital picture when they come out of the processor. I want to check my > >problems cases tomorrow. We do not use sponges but the only other like > >was the PA who was wrapping the blue paper very tight around the tissue. > >I really do not think this is the issue though.. Any other insight would > >be greatly appreciated. > > > >Susan T. Paturzo > >TJUH > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jpiche <@t> wtbyhosp.org Wed Apr 22 11:24:28 2015 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Wed Apr 22 11:24:34 2015 Subject: [Histonet] histology in higher education In-Reply-To: References: <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net>, <20150422143156.21929.qmail@server276.com> , <6B106EE8C8AAEF449DEA97921DEC11670E160222D2@EXMBOX-VP05.OSUMC.EDU> Message-ID: <631955447A364B45B9458D2905635110D8BF681F@WIN08-MBX-01.wtbyhosp.org> Thank you for sharing this information Andi. I'd like to do something like this and I'm going to send this on to my daughters science teachers at school. I think it's a great idea. It always amazed me all the different jobs in hospitals alone that are available for kids and adults alike and no one knows they exist. Especially histology. Looking forward to passing this on! Thanks again, Jessica Piche, HT(ASCP) Waterbury Hospital CT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 12:01 PM To: "" Subject: RE: [Histonet] histology in higher education Bonnie, and anybody who wants to do this: www.prescientist.org ________________________________________ From: Whitaker, Bonnie [Bonnie.Whitaker@osumc.edu] Sent: Wednesday, April 22, 2015 8:45 AM To: Grantham, Andrea L - (algranth); "" Subject: RE: [Histonet] histology in higher education Andi, Would you be willing to share the information on how to volunteer with this program? Thanks, Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 11:40 AM To: "" Subject: RE: [Histonet] histology in higher education For a few years I've been involved in a program called "letters to a pre-scientist". The idea is to reach middle schoolers as they are being introduced to the sciences. They have pretty high goals at this time, they want to be doctors and astronauts and engineers but they are just starting to learn about these things. You become a pen pal/mentor of sorts and write letters to a child and they will write back to you. Last year I was writing to a boy in the Chicago area and this year it was a girl in LA. I always write about what I do and how important it is and include pictures of things like brain cells, muscle, fungus, bacteria and pictures of my lab. I always pick up a copy of the NSH coloring book and send it to them and tell them what they need to study to be a histotech and other than a hospital, where they can find a job. Of course we also tell them about other things like our families, pets, vacations, etc. at the same time. It's just a small thing but it plants a seed. Andi G. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of wsimons@athensgastro.com [wsimons@athensgastro.com] Sent: Wednesday, April 22, 2015 7:31 AM To: koellingr@comcast.net; "" Subject: Re: [Histonet] histology in higher education Good morning Ray and thank you for promoting the field of Histotechnology. While President of the NSH, Vinnie DellaSperanza started a career day function at the annual NSH symposium. It has been very successful and the individuals that contribute to this volunteer effort are usually the same individuals that participate at the state and regional level. Thank you for the idea of state fairs and other avenues for the target age of middle & high school students. I did this myself when my children were in girl scouts , "Odyssey of the Mind" and advance placement opportunities. Wanda K. Simons, HT (ASCP) GSH President www.histosearch.com/gsh/ > -------Original Message------- > From: koellingr@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] histology in higher education > Sent: Apr 22 '15 10:14 > > The following has to do with histology and STEM (science, technology, engineering, math) so if not interested, please ignore. But I believe it can have real meaning to the profession of histology at the NSH, state society and local levels. > > I am elected to the Board of WSSEF (Washington State Science and Engineering Fair) where I am in educational outreach and also the assistant to the head judge. We recently had our Washington State Fair with 650 kids, grades 1-12 from all over the state. And while there was a lot of engineering and robots and computers there were a few projects having to do with medicine, biotechnology, immunology and pathology with some familiar histology or immunohistochemistry pictures included. At the end of the fair, we awarded almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 students. Not only that, our top winners get an all-expenses paid trip to present at the ISEF (Intel International Fair) with 1,700 students competing from all 50 states and 70 countries. Wherever you are in the US, you have a state fair. > > I would advocate for some of you so interested at the national, state or local levels to promote histology, by getting involved as mentors for middle and high school students to science fairs; especially those that could lead to histopathology or other related projects that could lead into Intel affiliated fairs resulting in great benefit to the student and a spread of the word of histology into both the STEM world and general population. > > I've mentored for 15 years. It can be done. Molecular histopathology, personalized diagnostics and therapeutics, advances in immunohistochemistry, current controversies about breast biopsy diagnosis, or other disease with newer classifications, PCR and RTPCR in histology, modern-targeted therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image analysis software for you computer geeks and on and on; the list is nearly limitless. Especially if you are close to or can contact biotech companies or educational institutions to find co-mentors for grades 7-12 there are histology-related science project possibilities in terms of data collection and the scientific method and project presentation are nearly unlimited now. > > Be a mentor for or engage a grade 7-12 student, with the help of another mentor or organization, to think about (histology-related) projects for science fairs leading to a state fair and Intel ISEF. Can't think of any better way to "promote histology" so would hope those at NSH would take note of this. And since the ISEF fair receives projects and groups from 70 countries, I hope any outside the US would also think about the same thing. > > Ray Koelling > HT, HTL, QIHC, STEM educational outreach advocate Lake Forest Park, > WA _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKb > mfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=iMDE > ff-OZMAAjL9OtBShZDX-UJn7oIfNUV_V5o3qIXg&s=DVIxzRUB8wWBw-770jBXVTA-wfsm > LPuP6XLZT68y1Ys&e= > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=iMDEff-OZMAAjL9OtBShZDX-UJn7oIfNUV_V5o3qIXg&s=DVIxzRUB8wWBw-770jBXVTA-wfsmLPuP6XLZT68y1Ys&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=iMDEff-OZMAAjL9OtBShZDX-UJn7oIfNUV_V5o3qIXg&s=DVIxzRUB8wWBw-770jBXVTA-wfsmLPuP6XLZT68y1Ys&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From kkienitz <@t> orclinic.com Wed Apr 22 12:39:48 2015 From: kkienitz <@t> orclinic.com (Kienitz, Kari) Date: Wed Apr 22 12:39:56 2015 Subject: [Histonet] Nuclear "Artifact" In-Reply-To: References: , Message-ID: <41400FFE517878449D89114DD2526090196602D0F8@tocmail1.tocad.orclinic.com> I have experience the over-cooked, dried out look a few times myself through the years and it can be baffeling especially when pathologists tell you things look "dried out". The first thing we think of it too much exposure to heat/chemical when its almost always not enough exposure to xylene at the deparaffinization stage or the tissue is underprocessed. Both of these scenarios can make tissue looked dried out. Make sure your protocols are adequate for processing thickness. Then there are the varying paraffin compounds. the higher the polymer content the more time you need in xylene....I know it sounds crazy but increase your time in xylene prior to staining and you will see alot of your random staining issues disappear. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz@orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreightl@gmail.com] Sent: Wednesday, April 22, 2015 9:10 AM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Lisa Roy Subject: Re: [Histonet] Nuclear "Artifact" At a previous job, we found (this was specific to prostate biopsies) that one of our clients was taking the prostate biopsies out, lining them up on a dry paper towel, doing the whole procedure, then putting them into the formalin jars. It can be some time between the start and finish of a procedure, so the first couple were looking very dried out (paper towel absorbed most of the moisture) with very pale hematoxylin staining. The pathologist found them almost uninterpretable. When this happened, the first natural area we examined was processing, which in this case turned out not to be the culprit. Collection procedures are not usually done by lab staff, a clinician or staff may have a great idea to make things easier but not know the downstream effects. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Wed, Apr 22, 2015 at 10:02 AM, Michael Ann Jones wrote: > Happy Lab Week!! > We worked on our H&E for almost two years. We were using Leica H&E > products and after 2 years of struggling, adjusting and analyzing > everything in our lab - we switched to the Richard-Allen Scientific > products. We were seeing variability between days of staining, tissues > right next to each other, nuclear paleness, eosin uniformity instead of > differentiated, etc. Switching reagents helped us tremendously - we have > more consistent higher quality stains on a daily basis and within tissues. > > I you?re struggling and have analyzed your processors, etc. to death - > maybe try different reagents? (we even measured the tap water that we use > on our stainer daily, the pH of reagents every other hour etc. and between > 5 experienced histotechs, we couldn?t figure it out) > Good luck! :) > > Michael Ann > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > > > > On 4/21/15, 5:55 PM, "Sue" wrote: > > >OMG we are experiencing the same issue. At first it was just GI and now > >we are seeing it on prostate. One pathologist said it looks like the > >tissue has been cooked. The only issue is we can have two biopsies right > >next to one another in the basket one looks good and one looks bad. My > >director also thinks it is the processors. I had Thermo out and they > >could find nothing. We changed out all the reagents and the biopsies were > >fine than two days later we had some bad ones. I know in July Fisher had > >a formalin recall associated to the mixture of buffer, water and > >formalin. We thought that might be it but it is now almost a year later > >and all the bad formalin should be gone. The histotechs say the tissue is > >crunchy and they are right. I am running a test tonight of a small needle > >biopsy that I made from a colon. I placed it is straight formaldehyde > >overnight and am processing it on our biopsy cycle tonight. My director > >also wanted us to only put three levels on our Thermo, but he wanted the > >middle level to have empty baskets. I stopped that today because I think > >the other issue is that the poor biopsies may be on the top level and as > >the reagents are used the level changes, and also due to displacement > >with the middle level being empty the reagent levels may not reach the > >top. We just do not have the manpower to inspect every reagent every day, > >we have 6 processor and it would take a tech all day. We actually take a > >digital picture when they come out of the processor. I want to check my > >problems cases tomorrow. We do not use sponges but the only other like > >was the PA who was wrapping the blue paper very tight around the tissue. > >I really do not think this is the issue though.. Any other insight would > >be greatly appreciated. > > > >Susan T. Paturzo > >TJUH > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rarbaugh <@t> csdermatology.com Wed Apr 22 13:29:56 2015 From: rarbaugh <@t> csdermatology.com (Arbaugh, Roberta) Date: Wed Apr 22 13:30:03 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <761E2B5697F795489C8710BCC72141FF36805FC3@ex07.net.ucsf.edu> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> <761E2B5697F795489C8710BCC72141FF36805FC3@ex07.net.ucsf.edu> Message-ID: <469FD9C7F82DC749A414F241CB0474671F9F9534@EXCHANGE02.ohpin.com> We have had the same problem. We come to the conclusion that it was water droplets. We had the problem when our humidity was high in the lab, or our weekend run. The changes we made where : 1. We no longer process over the weekend . We cannot count on our heating and cooling in our building. 2. We place a towel and a thick piece of cardboard on top of the processor lid. 3.We do not use the really fine mesh cassettes. We will use formalin soaked sponges or perm papers. 4. We do not over pack cassette basket. We had our processor check every time we saw the artifact and they could never find a problem with the processor. Hope this help, Roberta -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Wednesday, April 22, 2015 11:16 AM To: Sue; Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Nuclear "Artifact" "One pathologist said it looks like the tissue has been cooked." Which could also be drying artifact after bx, before formalin. "The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors." Same processor but two results? Solving intermittent problems takes time to check variables - time almost no one wants to spend to check out every possibility. But if one variable is the same for both, and the result is different, then most likely it is a different variable causing the problem. I think the other idea suggested of checking the handling of the tissue at the source of the biopsy is more likely to shed some light on this issue. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, April 21, 2015 4:55 PM To: Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear "Artifact" OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. Susan T. Paturzo TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. From joelleweaver <@t> hotmail.com Wed Apr 22 13:51:14 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Wed Apr 22 13:51:18 2015 Subject: [Histonet] NY State regulations In-Reply-To: <3652170FF5F22544A1280F1DB5D9199416F4284316@NJUE1.nj-urology.com> References: <3652170FF5F22544A1280F1DB5D9199416F4284316@NJUE1.nj-urology.com> Message-ID: Probably CLIA related to high complexity testing. IHC is not considered under CLIA ( from 1988), though many people feel otherwise. Grossing is. I think that it is under sub part G or H if I remember correctly. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: gmarcella@nj-urology.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 22 Apr 2015 10:55:19 -0400 > Subject: [Histonet] NY State regulations > > Hi - I was wondering if anyone knows the regulations regarding the NY State Clinical Laboratory license. I have been a Histotech and have worked in IHC for 20+ years and was required to obtain a NY State Clinical Lab License in 2007. I don't have and associates or bachelor degree and was not required to prior to 2007. I was told on a job interview that if I don't have either of these degrees that I cannot gross any specimens or run IHC. I've never heard this before. Has anyone else ever heard of this??? Thanks - Gail > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Apr 22 13:53:14 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Wed Apr 22 13:53:20 2015 Subject: [Histonet] histology in higher education In-Reply-To: <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net> References: <1886624111.8110643.1429709352958.JavaMail.zimbra@comcast.net>, <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net> Message-ID: Many people do this, and have donated hundreds of hours of their own time. But definately not enough. Good to encourage people to get involved. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 22 Apr 2015 14:07:18 +0000 > From: koellingr@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] histology in higher education > > The following has to do with histology and STEM (science, technology, engineering, math) so if not interested, please ignore. But I believe it can have real meaning to the profession of histology at the NSH, state society and local levels. > > I am elected to the Board of WSSEF (Washington State Science and Engineering Fair) where I am in educational outreach and also the assistant to the head judge. We recently had our Washington State Fair with 650 kids, grades 1-12 from all over the state. And while there was a lot of engineering and robots and computers there were a few projects having to do with medicine, biotechnology, immunology and pathology with some familiar histology or immunohistochemistry pictures included. At the end of the fair, we awarded almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 students. Not only that, our top winners get an all-expenses paid trip to present at the ISEF (Intel International Fair) with 1,700 students competing from all 50 states and 70 countries. Wherever you are in the US, you have a state fair. > > I would advocate for some of you so interested at the national, state or local levels to promote histology, by getting involved as mentors for middle and high school students to science fairs; especially those that could lead to histopathology or other related projects that could lead into Intel affiliated fairs resulting in great benefit to the student and a spread of the word of histology into both the STEM world and general population. > > I've mentored for 15 years. It can be done. Molecular histopathology, personalized diagnostics and therapeutics, advances in immunohistochemistry, current controversies about breast biopsy diagnosis, or other disease with newer classifications, PCR and RTPCR in histology, modern-targeted therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image analysis software for you computer geeks and on and on; the list is nearly limitless. Especially if you are close to or can contact biotech companies or educational institutions to find co-mentors for grades 7-12 there are histology-related science project possibilities in terms of data collection and the scientific method and project presentation are nearly unlimited now. > > Be a mentor for or engage a grade 7-12 student, with the help of another mentor or organization, to think about (histology-related) projects for science fairs leading to a state fair and Intel ISEF. Can't think of any better way to "promote histology" so would hope those at NSH would take note of this. And since the ISEF fair receives projects and groups from 70 countries, I hope any outside the US would also think about the same thing. > > Ray Koelling > HT, HTL, QIHC, STEM educational outreach advocate > Lake Forest Park, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf <@t> gmail.com Wed Apr 22 14:26:02 2015 From: garreyf <@t> gmail.com (Garreyf) Date: Wed Apr 22 14:26:07 2015 Subject: [Histonet] NY State regulations In-Reply-To: <3652170FF5F22544A1280F1DB5D9199416F4284316@NJUE1.nj-urology.com> References: <3652170FF5F22544A1280F1DB5D9199416F4284316@NJUE1.nj-urology.com> Message-ID: <0DF4DE11-93C1-442A-941E-45C6F29BB6CF@gmail.com> I believe grossing of small biopsies and performing ihc are both considered high complex testing. You must fulfill the clia personnel requirements of high complex testing. I also believe a histotech who only cuts and performs routine stains is not considered highly complex. I'm not sure why? Anyone know? Garrey Sent from my iPhone > On Apr 22, 2015, at 10:55 AM, Gail Marcella wrote: > > Hi - I was wondering if anyone knows the regulations regarding the NY State Clinical Laboratory license. I have been a Histotech and have worked in IHC for 20+ years and was required to obtain a NY State Clinical Lab License in 2007. I don't have and associates or bachelor degree and was not required to prior to 2007. I was told on a job interview that if I don't have either of these degrees that I cannot gross any specimens or run IHC. I've never heard this before. Has anyone else ever heard of this??? Thanks - Gail > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt <@t> uphs.upenn.edu Wed Apr 22 14:33:35 2015 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Wed Apr 22 14:33:41 2015 Subject: [Histonet] NY State regulations In-Reply-To: <0DF4DE11-93C1-442A-941E-45C6F29BB6CF@gmail.com> References: <3652170FF5F22544A1280F1DB5D9199416F4284316@NJUE1.nj-urology.com> <0DF4DE11-93C1-442A-941E-45C6F29BB6CF@gmail.com> Message-ID: <14D6A469D20B4F4AACC47C3E973C3FA8CFE071@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Just a CLIA reg, but you are correct microtomy, embedding and routine stains are only Moderate Complexity testing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garreyf Sent: Wednesday, April 22, 2015 3:26 PM To: Gail Marcella Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NY State regulations I believe grossing of small biopsies and performing ihc are both considered high complex testing. You must fulfill the clia personnel requirements of high complex testing. I also believe a histotech who only cuts and performs routine stains is not considered highly complex. I'm not sure why? Anyone know? Garrey Sent from my iPhone > On Apr 22, 2015, at 10:55 AM, Gail Marcella wrote: > > Hi - I was wondering if anyone knows the regulations regarding the NY State Clinical Laboratory license. I have been a Histotech and have worked in IHC for 20+ years and was required to obtain a NY State Clinical Lab License in 2007. I don't have and associates or bachelor degree and was not required to prior to 2007. I was told on a job interview that if I don't have either of these degrees that I cannot gross any specimens or run IHC. I've never heard this before. Has anyone else ever heard of this??? Thanks - Gail > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Apr 22 15:17:50 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Apr 22 15:18:06 2015 Subject: [Histonet] histology in higher education In-Reply-To: References: <1886624111.8110643.1429709352958.JavaMail.zimbra@comcast.net> <1311759885.8142747.1429711638580.JavaMail.zimbra@comcast.net> Message-ID: <272689740.8458973.1429733869995.JavaMail.zimbra@comcast.net> Hi, Thanks for info?and happy to hear that.? Didn't realize.? May 10-15 is Worlds largest International Science and Engineering?Fair in Pittsburgh this year?and maybe will see others there and watch a high school histology project from somewhere bring home a $75,000 scholarship.? ? Ray ----- Original Message ----- From: "Joelle Weaver" To: koellingr@comcast.net, histonet@lists.utsouthwestern.edu Sent: Wednesday, April 22, 2015 11:53:14 AM Subject: RE: [Histonet] histology in higher education Many people do this, and have donated hundreds of hours of their own time. But definately not enough. Good to encourage people to get involved. Joelle Weaver MAOM, HTL (ASCP) QIHC ???????? ?? ? > Date: Wed, 22 Apr 2015 14:07:18 +0000 > From: koellingr@comcast.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] histology in higher education > > The following has to do with histology and STEM (science, technology, engineering, math) so if not interested, please ignore.? But I believe it?can have?real meaning to the profession of histology at the NSH, state society and local levels. > ? > I am elected to the Board of WSSEF (Washington State Science and Engineering Fair) where I am in educational outreach and?also the assistant to the head judge.? We recently had our Washington State Fair with 650 kids, grades 1-12 from all over the state.? And while there was a lot of engineering and robots and computers there were a few projects having to do with medicine, biotechnology, immunology and pathology with some familiar?histology or immunohistochemistry pictures included.? At the end of the fair, we awarded almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 students.? Not only that, our top winners get an all-expenses paid trip to present at the ISEF (Intel International Fair) with 1,700 students competing from all 50 states and 70 countries.? Wherever you are in the US, you have a state fair. > ? > I would advocate for some of you so interested at the national, state or local levels to promote histology,?by getting?involved as mentors for middle and high school students to science fairs; especially those that could lead to histopathology or other related projects that could lead into Intel affiliated fairs resulting in great benefit to the student and a spread of the word of histology into both the STEM world and general population. > ? > I've mentored for 15 years.? It can be done.? Molecular histopathology, personalized diagnostics and therapeutics, advances in immunohistochemistry, current controversies about breast biopsy diagnosis, or other disease with newer classifications, PCR and RTPCR in histology, modern-targeted therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image analysis software for you computer geeks and on and on; the list is nearly limitless.? Especially if you are close to or can contact biotech companies or educational institutions to find co-mentors for grades 7-12 there are histology-related science project possibilities in terms of data collection and the scientific method and project presentation are nearly unlimited now. > ? > Be a mentor for or engage a grade 7-12 student, with the help of another mentor or organization, to think about (histology-related) projects for science fairs leading to a state fair and Intel ISEF.? Can't think of any better way to "promote histology" so would hope those at NSH would take note of this.? And since the ISEF fair receives projects and groups from 70 countries, I hope any outside the US would also think about the same thing. > ? > Ray Koelling > HT, HTL, QIHC, STEM educational outreach advocate > Lake Forest Park, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Apr 22 15:32:54 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Wed Apr 22 15:32:58 2015 Subject: [Histonet] NY State regulations In-Reply-To: <14D6A469D20B4F4AACC47C3E973C3FA8CFE071@UPHMASPHI030.UPHS.PENNHEALTH.PRV> References: <3652170FF5F22544A1280F1DB5D9199416F4284316@NJUE1.nj-urology.com>, <0DF4DE11-93C1-442A-941E-45C6F29BB6CF@gmail.com>, <14D6A469D20B4F4AACC47C3E973C3FA8CFE071@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Message-ID: The FDA categorizes and grades each test based on the complexity of the test method. The FDA lists the category at http://www.fda.gov/MedicalDevices/DeviceRegulationandGuidance/IVDRegulatoryAssistance/ucm393285.htm on the FDA website. The FDA categorizes test methods into three levels of complexity: Waived complexity, Moderate Complexity, including the subcategory of Provider-Performed Microscopy Procedures (PPMP); and High Complexity.When categorizing a test, the FDA considers the: Amount of interpretation involved; Calibration and quality control requirements of the instruments used; Degree of independent judgment involved; Difficulty of the calculations involved; Examinations and procedures performed and the methodologies employed; and Type of training required to operate the instruments used in the methodology. How is it determined if a test is waived, moderate or high complexity? For moderate and high complexity tests, the FDA evaluates each new commercial test system during the premarket approval process by scoring seven criteria as described in the CLIA regulations. The final score is used to determine whether the test system is classified as moderate or high complexity. See 42 CFR 493.17. For more details, please also see the FDA?s webpage on the CLIA Categorization Criteria and CMS? webpage on Categorization of Tests. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Caroline.Pratt@uphs.upenn.edu > To: garreyf@gmail.com; gmarcella@nj-urology.com > Date: Wed, 22 Apr 2015 19:33:35 +0000 > Subject: RE: [Histonet] NY State regulations > CC: histonet@lists.utsouthwestern.edu > > Just a CLIA reg, but you are correct microtomy, embedding and routine stains are only Moderate Complexity testing. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garreyf > Sent: Wednesday, April 22, 2015 3:26 PM > To: Gail Marcella > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] NY State regulations > > I believe grossing of small biopsies and performing ihc are both considered high complex testing. You must fulfill the clia personnel requirements of high complex testing. > > I also believe a histotech who only cuts and performs routine stains is not considered highly complex. I'm not sure why? Anyone know? > > Garrey > > Sent from my iPhone > > > On Apr 22, 2015, at 10:55 AM, Gail Marcella wrote: > > > > Hi - I was wondering if anyone knows the regulations regarding the NY State Clinical Laboratory license. I have been a Histotech and have worked in IHC for 20+ years and was required to obtain a NY State Clinical Lab License in 2007. I don't have and associates or bachelor degree and was not required to prior to 2007. I was told on a job interview that if I don't have either of these degrees that I cannot gross any specimens or run IHC. I've never heard this before. Has anyone else ever heard of this??? Thanks - Gail > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From khong <@t> waxitinc.com Wed Apr 22 15:52:19 2015 From: khong <@t> waxitinc.com (khong@waxitinc.com) Date: Wed Apr 22 15:52:24 2015 Subject: [Histonet] Technovit 9100 new Message-ID: Hi, it there anyone who has experience in Technovit 9100 new? I'm having a hard time in polymerization process using T9100 now. I just try to follow the manufacture instruction but it didn't work. Pls give me an advice or suggestion. Thanks, Kai H From thisisann <@t> aol.com Wed Apr 22 15:53:43 2015 From: thisisann <@t> aol.com (Ann Specian) Date: Wed Apr 22 15:53:48 2015 Subject: [Histonet] Lifting sections off of slides Message-ID: <14ce2e86659-55b2-c950@webprd-m34.mail.aol.com> Does anyone have a procedure where in you can lift the stained sections off of a slide and place them on another slide? Sent from AOL Mobile Mail From garreyf <@t> gmail.com Wed Apr 22 17:10:18 2015 From: garreyf <@t> gmail.com (Garrey Faller) Date: Wed Apr 22 17:10:21 2015 Subject: [Histonet] NY State regulations In-Reply-To: References: <3652170FF5F22544A1280F1DB5D9199416F4284316@NJUE1.nj-urology.com> <0DF4DE11-93C1-442A-941E-45C6F29BB6CF@gmail.com> <14D6A469D20B4F4AACC47C3E973C3FA8CFE071@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Message-ID: Check this link out from CAP. http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf Scroll down to #17. It address IHC. Garrey On Wed, Apr 22, 2015 at 4:32 PM, Joelle Weaver wrote: > The FDA categorizes and grades each test based on the complexity of the > test method. The FDA lists the category at > > *http://www.fda.gov/MedicalDevices/DeviceRegulationandGuidance/IVDRegulatoryAssistance/ucm393285.htm > > *on the FDA website. The FDA categorizes test methods into three levels > of complexity: > > Waived complexity, Moderate Complexity, including the subcategory of > Provider-Performed Microscopy Procedures (PPMP); and High Complexity. > > When categorizing a test, the FDA considers the: > > > > > 1. Amount of interpretation involved; > 2. Calibration and quality control requirements of the instruments > used; > 3. Degree of independent judgment involved; > 4. Difficulty of the calculations involved; > 5. Examinations and procedures performed and the methodologies > employed; and > 6. Type of training required to operate the instruments used in the > methodology. > > > How is it determined if a test is waived, moderate or high complexity? > For moderate and high complexity tests, the FDA evaluates each new > commercial test system during the premarket approval process by scoring > seven criteria as described in the CLIA regulations. The final score is > used to determine whether the test system is classified as moderate or high > complexity. See 42 CFR 493.17. For more details, please also see the FDA?s > webpage on the CLIA Categorization Criteria > [image: > External Web Site Icon] and > CMS? webpage on Categorization of Tests > [image: > External Web Site Icon] . > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > From: Caroline.Pratt@uphs.upenn.edu > > To: garreyf@gmail.com; gmarcella@nj-urology.com > > Date: Wed, 22 Apr 2015 19:33:35 +0000 > > Subject: RE: [Histonet] NY State regulations > > CC: histonet@lists.utsouthwestern.edu > > > > Just a CLIA reg, but you are correct microtomy, embedding and routine > stains are only Moderate Complexity testing. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garreyf > > Sent: Wednesday, April 22, 2015 3:26 PM > > To: Gail Marcella > > Cc: histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] NY State regulations > > > > I believe grossing of small biopsies and performing ihc are both > considered high complex testing. You must fulfill the clia personnel > requirements of high complex testing. > > > > I also believe a histotech who only cuts and performs routine stains is > not considered highly complex. I'm not sure why? Anyone know? > > > > Garrey > > > > Sent from my iPhone > > > > > On Apr 22, 2015, at 10:55 AM, Gail Marcella > wrote: > > > > > > Hi - I was wondering if anyone knows the regulations regarding the NY > State Clinical Laboratory license. I have been a Histotech and have worked > in IHC for 20+ years and was required to obtain a NY State Clinical Lab > License in 2007. I don't have and associates or bachelor degree and was not > required to prior to 2007. I was told on a job interview that if I don't > have either of these degrees that I cannot gross any specimens or run IHC. > I've never heard this before. Has anyone else ever heard of this??? Thanks > - Gail > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald <@t> mtsac.edu Wed Apr 22 17:33:35 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Apr 22 17:33:44 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <469FD9C7F82DC749A414F241CB0474671F9F9534@EXCHANGE02.ohpin.com> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> <761E2B5697F795489C8710BCC72141FF36805FC3@ex07.net.ucsf.edu> <469FD9C7F82DC749A414F241CB0474671F9F9534@EXCHANGE02.ohpin.com> Message-ID: Our pathologists also complained about some of the GI biopsies looking burnt. We tracked all of the problem cases back to one histotech. The histotech was causing the "burn" artifact with excessive use of freezing spray. From: "Arbaugh, Roberta" To: "'Morken, Timothy'" , Sue , Lisa Roy Cc: "histonet@lists.utsouthwestern.edu" Date: 04/22/2015 11:32 AM Subject: RE: [Histonet] RE: Nuclear "Artifact" Sent by: histonet-bounces@lists.utsouthwestern.edu We have had the same problem. We come to the conclusion that it was water droplets. We had the problem when our humidity was high in the lab, or our weekend run. The changes we made where : 1. We no longer process over the weekend . We cannot count on our heating and cooling in our building. 2. We place a towel and a thick piece of cardboard on top of the processor lid. 3.We do not use the really fine mesh cassettes. We will use formalin soaked sponges or perm papers. 4. We do not over pack cassette basket. We had our processor check every time we saw the artifact and they could never find a problem with the processor. Hope this help, Roberta -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Wednesday, April 22, 2015 11:16 AM To: Sue; Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Nuclear "Artifact" "One pathologist said it looks like the tissue has been cooked." Which could also be drying artifact after bx, before formalin. "The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors." Same processor but two results? Solving intermittent problems takes time to check variables - time almost no one wants to spend to check out every possibility. But if one variable is the same for both, and the result is different, then most likely it is a different variable causing the problem. I think the other idea suggested of checking the handling of the tissue at the source of the biopsy is more likely to shed some light on this issue. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, April 21, 2015 4:55 PM To: Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear "Artifact" OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. Susan T. Paturzo TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Apr 22 17:49:52 2015 From: mucram11 <@t> comcast.net (=?utf-8?B?bXVjcmFtMTE=?=) Date: Wed Apr 22 17:50:09 2015 Subject: [Histonet] RE: Nuclear "Artifact" Message-ID: <000f4242.4a7fb464460a352b@comcast.net> We don't even allow freeze spray in the lab. Sent from my Verizon 4G LTE Smartphone ------ Original message------ From: Jennifer MacDonald Date: Wed, Apr 22, 2015 5:34 PM To: Arbaugh, Roberta; Cc: histonet@lists.utsouthwestern.edu;Lisa Roy;histonet-bounces@lists.utsouthwestern.edu;'Morken, Timothy'; Subject:RE: [Histonet] RE: Nuclear "Artifact" Our pathologists also complained about some of the GI biopsies looking burnt. We tracked all of the problem cases back to one histotech. The histotech was causing the "burn" artifact with excessive use of freezing spray. From: "Arbaugh, Roberta" To: "'Morken, Timothy'" , Sue , Lisa Roy Cc: "histonet@lists.utsouthwestern.edu" Date: 04/22/2015 11:32 AM Subject: RE: [Histonet] RE: Nuclear "Artifact" Sent by: histonet-bounces@lists.utsouthwestern.edu We have had the same problem. We come to the conclusion that it was water droplets. We had the problem when our humidity was high in the lab, or our weekend run. The changes we made where : 1. We no longer process over the weekend . We cannot count on our heating and cooling in our building. 2. We place a towel and a thick piece of cardboard on top of the processor lid. 3.We do not use the really fine mesh cassettes. We will use formalin soaked sponges or perm papers. 4. We do not over pack cassette basket. We had our processor check every time we saw the artifact and they could never find a problem with the processor. Hope this help, Roberta -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Wednesday, April 22, 2015 11:16 AM To: Sue; Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Nuclear "Artifact" "One pathologist said it looks like the tissue has been cooked." Which could also be drying artifact after bx, before formalin. "The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors." Same processor but two results? Solving intermittent problems takes time to check variables - time almost no one wants to spend to check out every possibility. But if one variable is the same for both, and the result is different, then most likely it is a different variable causing the problem. I think the other idea suggested of checking the handling of the tissue at the source of the biopsy is more likely to shed some light on this issue. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, April 21, 2015 4:55 PM To: Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear "Artifact" OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. Susan T. Paturzo TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Wed Apr 22 18:47:09 2015 From: suetp918 <@t> comcast.net (Sue) Date: Wed Apr 22 18:47:34 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <000f4242.4a7fb464460a352b@comcast.net> References: <000f4242.4a7fb464460a352b@comcast.net> Message-ID: <1628099692.1387617.1429746429163.JavaMail.zimbra@comcast.net> we do not use freeze spray in the lab at all. this issue has really gotten me bummed out since it is so sporadic. I am going to try to see if it is associated to one day in particular. it is one PA but multiple techs have identified the issue. From jshelley <@t> sanfordburnham.org Thu Apr 23 09:51:06 2015 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Thu Apr 23 09:51:20 2015 Subject: [Histonet] Florida Society for Histotechnology Meeting Message-ID: Good Morning Histonetters! Final hotel reservations should be made no later than April 23rd, 2015 to ensure rate and availability. After today you will have to hope that room prices will not go up. I am working on trying to squeeze out a few more days for the hotel registration link below. Try this link below first and if it does not work make sure that when you call (1-866-397-6516) the hotel to make reservations that you mention Florida Society for Histotechnology. Act now to save money and possible aggravation. Looking forward to seeing you at our meeting! Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402&hotelID=6579 Need to book no later than 4-23-15 Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155&lbrd=1&rtypeid=380141 Have a great day! Kind Regards! John J Shelley 2014-2016 FSH President From relia1 <@t> earthlink.net Thu Apr 23 11:31:59 2015 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Apr 23 11:32:10 2015 Subject: [Histonet] Happy National Medical Laboratory Professionals Week 4-2015 From Pam Barker and RELIA Solutions Message-ID: <014701d07de3$05d0c2c0$11724840$@earthlink.net> Hi Histopeeps!! I hope you are doing well. I wanted to send a special bulletin wishing you a Happy National Medical Laboratory Professionals Week. I Hope Your Lab Has Been Having Some Fun, Informative & Special Events To Celebrate Because You Deserve It! If you are on Instagram or Twitter check out the hashtag #lab4life and post pics too!! Also I would love to hear what you are doing in your lab to celebrate so please shoot me back an e-mail and let me know. I have some exciting opportunities with some of my best clients located in TN,TX, AL, IN, IL, VA and NC. Every Day I'm Talking to Clients Nationwide About Amazing New Positions So Stay Tuned for Brand New EXCLUSIVE opportunities in locations all over the USA! I only work with companies that offer full time permanent positions, competitive pay, excellent benefits and a great place to work! If you or anyone you know is looking or thinking about a job change, please contact me ASAP!! I can be reached on my cell phone at 407-353-5070 or at relia1@earthlink.net or toll free at 866-607-3542 Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From lisalocum <@t> yahoo.co.uk Wed Apr 8 10:36:48 2015 From: lisalocum <@t> yahoo.co.uk (lisa mccluskey) Date: Thu Apr 23 12:15:33 2015 Subject: [Histonet] lisa mccluskey Message-ID: good morning, http://test.redlinegaragegear.com/gnfur/krptiaujleozhfavc.wnjzsqglblzutjyuvhwbbzbgaqhfxmvb From ltougas <@t> dawsoncollege.qc.ca Thu Apr 9 21:49:33 2015 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Thu Apr 23 12:15:37 2015 Subject: [Histonet] NSH region IX Message-ID: <455897B94CF3A44F81F727160F23FB11640C09E4@DC229.ad.dawsoncollege.qc.ca> Hi fellow Canadian and American Histonetters! Please find attached an invitation to attend our regional symposium on June 5th and 6th in Toronto! It may be noticed that the opening "wine and cheese" immediately follows the IQMH meeting on Friday night. Stay tuned for details about speakers and workhops! Liette Tougas, RT, B.Sc., M.Sc., Faculty Biomedical Laboratory Technology Department Dawson College, Montr?al, Que. From ltougas <@t> dawsoncollege.qc.ca Tue Apr 14 16:12:13 2015 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Thu Apr 23 12:15:40 2015 Subject: [Histonet] FW: NSH region IX In-Reply-To: <455897B94CF3A44F81F727160F23FB11640C09E4@DC229.ad.dawsoncollege.qc.ca> References: <455897B94CF3A44F81F727160F23FB11640C09E4@DC229.ad.dawsoncollege.qc.ca> Message-ID: <455897B94CF3A44F81F727160F23FB11640C0EB0@DC229.ad.dawsoncollege.qc.ca> Hi fellow Canadian and American Histonetters! Please find attached an invitation to attend our regional symposium on June 5th and 6th in Toronto! It may be noticed that the opening "wine and cheese" immediately follows the IQMH meeting on Friday night. Stay tuned for details about speakers and workhops! Liette Tougas, RT, B.Sc., M.Sc., Faculty Biomedical Laboratory Technology Department Dawson College, Montr?al, Que. From pablo.sanchez <@t> usc.es Tue Apr 21 05:17:10 2015 From: pablo.sanchez <@t> usc.es (Pablo Sanchez-Quinteiro) Date: Thu Apr 23 12:15:42 2015 Subject: [Histonet] Eosin pH Message-ID: <0d1a8e$1rcbp7@correo2bi.usc.es> Listers, Could you tell me the best way to adjut the eosin pH to 4-5? Thanks in advance Pablo From mikael.niku <@t> helsinki.fi Tue Apr 21 06:27:49 2015 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Thu Apr 23 12:15:45 2015 Subject: [Histonet] Intestinal bacteria for paraffin sections - morphology? Message-ID: <55363435.7080706@helsinki.fi> Hi! I'm trying to produce paraffin sections showing intestinal bacteria in intestinal tissue sections. We have tried routine PFA fixation and paraffin processing, and a Carnoy-based protocol supposedly optimized for this purpose. The problem is, the bacteria seem to shrink so much that I can't see any nice shapes but only very tiny roughly circular objects. What to do? With best regards, Mikael Niku, University of Helsinki From gmarcella <@t> nj-urology.com Tue Apr 21 10:47:58 2015 From: gmarcella <@t> nj-urology.com (Gail Marcella) Date: Thu Apr 23 12:15:48 2015 Subject: [Histonet] NY State Histology License Message-ID: <3652170FF5F22544A1280F1DB5D9199411C2D2D4D2@NJUE1.nj-urology.com> Hi - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website. I was wondering if anyone else heard of this? Thanks - Gail From lisalocum <@t> yahoo.co.uk Thu Apr 23 06:34:41 2015 From: lisalocum <@t> yahoo.co.uk (lisa mccluskey) Date: Thu Apr 23 12:15:50 2015 Subject: [Histonet] 4/23/2015 11:34:41 AM Message-ID: <9818ECEC4A3A243B0AF60F0F50034125@mail.videopool.ch> http://progressnetzone.in/yrs/uxghqjyqiztglrskklcomidybvfojuwehwhfvtx.ohbckzpdyjvyjgndrfxgrnpgrbsozo 4/23/2015 11:34:41 AM From Cringler <@t> ironwooddermatology.com Thu Apr 16 15:56:46 2015 From: Cringler <@t> ironwooddermatology.com (Chris Ringler) Date: Thu Apr 23 12:19:25 2015 Subject: [Histonet] Can you please add me to this mailing list Message-ID: <3CDA6086D53F0A4B9ABA14F6C14F39258EDED8@core.ironwooddermatology.com> cringler@ironwooddermatology.com Respectfully, Chris Chris Ringler CEO Ironwood Dermatology PC 1735 E Skyline Dr Tucson AZ 85718 520-618-1630 ext. 106 I Fax 520-618-1636 Confidentiality Notice: This e-mail message contains information which may be confidential and privileged and is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521 and is legally privileged. Unauthorized review, use, disclosure or distribution is strictly prohibited. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by calling 520-618-1630 ext 106 or by reply e-mail, and destroy all copies of the message. Thank you very much. -- From b.maryottash <@t> gmail.com Fri Apr 17 12:35:53 2015 From: b.maryottash <@t> gmail.com (Bridget Maryott) Date: Thu Apr 23 12:19:28 2015 Subject: [Histonet] Arizona Society for Histotechnology 2015 Spring Meeting Message-ID: *Arizona Society for Histotechnology Spring Quarterly Meeting* *Saturday, May 9th* Sonora Quest Laboratories/Laboratory Sciences of Arizona 1255 W. Washington St. | Tempe, AZ 85281 8:00-11:30 *Anatomic Pathology and Quality Process Improvement* William DeSalvo Lunch and Social 11:30-1:00 1:00-4:30 *Are You A Control Freak?? Do You Have Control Of Your Pre-Analytics?*? Robert Lott Tanya Ewing-Finchem Please join us for a fun and educational day; continental breakfast and lunch will be provided. *To sign up, please go to: * *www.SignUpGenius.com/go/10C044FACAB23A1FE3-ashspring* -- *-Bridget Maryott, HT (ASCP)* From Joyce.Weems <@t> emoryhealthcare.org Thu Apr 23 12:24:26 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Apr 23 12:24:33 2015 Subject: [Histonet] unsubscribe In-Reply-To: References: Message-ID: Hi Anita, You must unsubscribe yourself through the link at the bottom of your email.. http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best wishes, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita Sent: Tuesday, March 31, 2015 11:45 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] unsubscribe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Joyce.Weems <@t> emoryhealthcare.org Thu Apr 23 12:51:41 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Apr 23 12:53:36 2015 Subject: [Histonet] RE: Can you please add me to this mailing list In-Reply-To: <3CDA6086D53F0A4B9ABA14F6C14F39258EDED8@core.ironwooddermatology.com> References: <3CDA6086D53F0A4B9ABA14F6C14F39258EDED8@core.ironwooddermatology.com> Message-ID: Hi Chris, You must go to http://lists.utsouthwestern.edu/mailman/listinfo/histonet and sign yourself up.. Welcome!!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Ringler Sent: Thursday, April 16, 2015 4:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Can you please add me to this mailing list cringler@ironwooddermatology.com Respectfully, Chris Chris Ringler CEO Ironwood Dermatology PC 1735 E Skyline Dr Tucson AZ 85718 520-618-1630 ext. 106 I Fax 520-618-1636 Confidentiality Notice: This e-mail message contains information which may be confidential and privileged and is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521 and is legally privileged. Unauthorized review, use, disclosure or distribution is strictly prohibited. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by calling 520-618-1630 ext 106 or by reply e-mail, and destroy all copies of the message. Thank you very much. -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From joelleweaver <@t> hotmail.com Thu Apr 23 13:34:13 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Thu Apr 23 13:34:17 2015 Subject: [Histonet] NY State Histology License In-Reply-To: <3652170FF5F22544A1280F1DB5D9199411C2D2D4D2@NJUE1.nj-urology.com> References: <3652170FF5F22544A1280F1DB5D9199411C2D2D4D2@NJUE1.nj-urology.com> Message-ID: Yes, CLIA stipulation. I think that there may be a grandfather clause, but not sure of the time frame. You could check the regulation on that. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: gmarcella@nj-urology.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 21 Apr 2015 11:47:58 -0400 > Subject: [Histonet] NY State Histology License > > Hi - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website. I was wondering if anyone else heard of this? Thanks - Gail > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Apr 23 13:37:03 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Thu Apr 23 13:37:08 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: References: , <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu>, <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org>, <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu>, <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net>, <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov>, <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu>, , , <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local>, <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local>, Message-ID: Yes, thank you! I hate when this statement is made. It is truly insulting. I have left organizations because the manager or director would say this right in meetings to us histotechs and to others in the meeting. At the moment of that utterance, I knew I was in the wrong place! Cheers. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: garreyf@gmail.com > Date: Tue, 24 Mar 2015 18:33:37 -0400 > To: Nancy.Stedman@buschgardens.com > Subject: Re: [Histonet] BS in Histotechnology > CC: histonet@lists.utsouthwestern.edu; mturner@CSILaboratories.com; JMacDonald@mtsac.edu; Timothy.Morken@ucsf.edu; PAMarcum@uams.edu > > I am trying to find (hire) a histotech and will make sure I don't use the words "trained monkey" in my interviews. . Ha ha. > I truly value and appreciate a skilled and motivated histotech. I've had to train myself to cut my own sections in order to understand the process better. It is a great field; it's like art to me. There is no room for monkeys in my book. > Garrey > > Sent from my iPhone > > > On Mar 24, 2015, at 5:22 PM, Stedman, Nancy wrote: > > > > As a pathologist I'd like to apologize for all the pathologists who have made comments like this.. forget trained monkeys and dogs, most (all?) pathologists cannot cut slides either, at least not slides they'd want to try to read. I know I can't. > > > > -Nancy Stedman > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner > > Sent: Tuesday, March 24, 2015 4:26 PM > > To: Paula Sicurello; Michael Ann Jones > > Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; Marcum, Pamela A > > Subject: RE: [Histonet] BS in Histotechnology > > > > I once worked with a Pathologist who said she was in a group meeting of other pathologists when one of them blurted out that a trained monkey could cut slides. My pathologist, having had the opportunity to review some cases from the offender's laboratory, promptly replied "Yes, and with the quality of your slides it looks like you did just that." She shut down the other pathologist really quickly, and as far as I know, we never received another case to review from him. My pathologist was not about to let that kind of arrogance stand. She was one of the best bosses I ever had! > > > > Mark Turner, Ph.D., HT(ASCP)QIHC > > Manager, Histology/IHC > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > > Sent: Tuesday, March 24, 2015 3:47 PM > > To: Michael Ann Jones > > Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; Timothy Morken > > Subject: Re: [Histonet] BS in Histotechnology > > > > I've had more than one pathologist tell me a monkey could do my job. > > Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. > > > > How many of us monkeys have trained the whining and complaining residents how to do things correctly? > > > > Paula > > > > On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones > > wrote: > > > >> OMG Pam~ our pathologist said the exact same thing to us when we > >> started our Grossing training. > >> Sheesh. . . > >> Michael Ann > >> > >> > >> > >> > >>> On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > >>> > >>> That was nicer than the pathologist who told me years ago, "any > >>> monkey could be trained to do my job". I basically did not take the > >>> job I was interviewing for at the time. At least the next interview > >>> went a lot better and I did take the job. > >>> > >>> Pam > >>> > >>> -----Original Message----- > >>> From: histonet-bounces@lists.utsouthwestern.edu > >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >>> Sanders, Jeanine (CDC/OID/NCEZID) > >>> Sent: Tuesday, March 24, 2015 12:30 PM > >>> To: Sue; Timothy Morken > >>> Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >>> Subject: RE: [Histonet] BS in Histotechnology > >>> > >>> I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can > >>> teach any trained dog how to section histology will never have the > >>> recognition those of us that have studied and trained deserve. > >>> > >>> -----Original Message----- > >>> From: histonet-bounces@lists.utsouthwestern.edu > >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >>> Sent: Tuesday, March 24, 2015 12:59 PM > >>> To: Timothy Morken > >>> Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >>> Subject: Re: [Histonet] BS in Histotechnology > >>> > >>> This is a fight that we continue to have with hospital administration. > >>> In my opinion histologists are just as important and needed as MT. > >>> Even though there is an increase in automation in pathology the hands > >>> on of a histologists is most important. The fact that hospital still > >>> consider a lower entry job is the reason there are not more of us. > >>> It is quite frustrating. > >>> > >>> Sue > >>> TJUH > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> --------------------------------------------------------------------- > >>> - Confidentiality Notice: This e-mail message, including any > >>> attachments, is for the sole use of the intended recipient(s) and may > >>> contain confidential and privileged information. Any unauthorized > >>> review, use, disclosure or distribution is prohibited. If you are not > >>> the intended recipient, please contact the sender by reply e-mail and > >>> destroy all copies of the original message. > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thomas_jackson <@t> unc.edu Thu Apr 23 14:21:48 2015 From: thomas_jackson <@t> unc.edu (Jackson, Thomas) Date: Thu Apr 23 14:21:54 2015 Subject: [Histonet] Blood in neural tissue a confound for DNA methylation data? Message-ID: <69AE72F61F805B48A33AFAA2A35E45A8B5A9FE@ITS-MSXMBS3M.ad.unc.edu> Hello histotechs! I am fully aware that this question doesn't fall under the specialty of histology, but I'm hoping that someone might be able to point me in the right direction! My lab is looking at DNA methylation in brain tissue (as well as sectioning frozen tissue on a cryostat to run IHC, but that is not our current issue- I will likely be seeking some help on that front in the future!). We have been sacrificing via a transcardiac perfusion, leading with a PBS wash and then followed by 4% formaldehyde in PBS. We have decided to cut the formaldehyde out of the process since the DNA methylation is the most important data for this study. We were then wondering if the PBS wash would still be a necessary step? The PBS wash would eliminate blood from the brain, but we were unsure if that is a necessary step or if "fresh" tissue (with blood) would still give us good DNA methylation results. Any advice or guidance in this would be greatly appreciated! Thanks, Thomas ________________________________ Thomas Jackson Research Technician Cheatham Nutrition and Cognition Lab UNC-CH Nutrition Research Institute 500 Laureate Way Kannapolis, NC 28081 704-250-5028 thomas_jackson@unc.edu www.uncnri.org From jvickroy <@t> SpringfieldClinic.com Thu Apr 23 15:19:21 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Thu Apr 23 15:19:28 2015 Subject: [Histonet] Question on IHC billing Message-ID: <9B1A1501A800064397369BD8072E6BCAB5C90C@E2K10DB.springfieldclinic.com> Let me see if I have this straight: If a pathologist orders an Hpylori stain on 2 blocks from the same specimen C1 and C2 we can only bill one 88342. If this correct. Obviously if he ordered addition different IHC stains we could change additional 88341's. Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Joyce.Weems <@t> EmoryHealthcare.org Thu Apr 23 15:31:09 2015 From: Joyce.Weems <@t> EmoryHealthcare.org (Weems, Joyce K.) Date: Thu Apr 23 15:31:19 2015 Subject: [Histonet] RE: Question on IHC billing In-Reply-To: <9B1A1501A800064397369BD8072E6BCAB5C90C@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCAB5C90C@E2K10DB.springfieldclinic.com> Message-ID: Correct Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Thursday, April 23, 2015 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on IHC billing Let me see if I have this straight: If a pathologist orders an Hpylori stain on 2 blocks from the same specimen C1 and C2 we can only bill one 88342. If this correct. Obviously if he ordered addition different IHC stains we could change additional 88341's. Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Luis.Chiriboga <@t> nyumc.org Thu Apr 23 13:00:10 2015 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Thu Apr 23 16:00:10 2015 Subject: [Histonet] Help with a survey on research antibodies Message-ID: <3E6798F00C9F494399E96B720ECD14293FD786@MSGWCDCPMB22.nyumc.org> Dear Colleagues, You are all well aware of the reproducibility issues when using antibodies for IHC. I'm working with the Global Biological Standards Institute (GBSI) to address two important issues-1) cell culture authentication practices and 2) research antibodies. I have agreed to help GBSI distribute the surveys through this link http://surveys.mckinley-advisors.com/s3/LC to gather valuable information about how researchers view the best practices in each of these areas and what they see as the challenges and barriers to implementing those practices. GBSI will be analyzing the data and publishing the results later in 2015. I strongly encourage you to participate or to distribute to your staff for their participation. At the beginning of the survey (question #3), you will notice that there is a simple way to take one or both surveys. GBSI's pretesting indicates that each survey can be completed in as little as 10 minutes. Please contact Mark Gibson at GBSI, mgibson@gbsi.org if you have specific questions about the survey instrument. Best regards and thanks for your time Luis Luis Chiriboga Ph.D, Director OCS Experimental Pathology IHC Core Lab NYU School of Medicine Smilow 308/310 Office: 646-501-6934 Mobile: (347) 712-0897 Luis.Chiriboga@nyumc.org From hiratama <@t> kuhp.kyoto-u.ac.jp Thu Apr 23 19:32:02 2015 From: hiratama <@t> kuhp.kyoto-u.ac.jp (=?UTF-8?B?5bmz55Sw5Yud5ZWTKEhpcmF0YU1hc2FoaXJvKQ==?=) Date: Thu Apr 23 19:32:27 2015 Subject: [Histonet] AEC visualization enhancement Message-ID: Hi, Can anyone please let me know how to increase AEC sensitivity ? I am using AEC for multiple IHC, but it is not particularly sensitive. My recepe is: 1% AEC in DMF 0.5ml 50mM acetate/sodium acetate buffer pH5.5 10ml 30% H2O2 5ul mix and filter incubate for 10-15min I m looking for enhancer (like imidazole for DAB) or more sensitive protocol for AEC. Thank you ?Hirata Masahiro Department of Diagnostic Pathology, Kyoto University Hospital hiratama@kuhp.kyoto-u.ac.jp From suetp918 <@t> comcast.net Thu Apr 23 19:51:16 2015 From: suetp918 <@t> comcast.net (Sue) Date: Thu Apr 23 19:51:26 2015 Subject: [Histonet] Nuclear "Artifact" In-Reply-To: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> Message-ID: <1471795104.1518386.1429836676486.JavaMail.zimbra@comcast.net> Hi All So I have been seeing the same issue as I stated in past e-mails. I did one test and fixed a colon biopsy in formaldehyde and left it in overnight and processed next day. I was hoping that I could reproduce the artifact. The tissue was beautiful. At my pathologists requests we changed the paraffin temperature tonight is the first night. I do not think this i the issue. We are going to transfer our biopsies to another tissue processor just for test. I brought up in the past that i think the issue starts prior to the histo lab, my pathologist tended to disagree, but I think he is chaining his mind since my one common detonator is a PA. I do not think that she wets her blue wrap paper enough and the tissue sits on the paper dry she also fold the paper so tight that it is possible for the small biopsies to get stuck in a fold. My pathologists actually came in and said he thought I may be right. Wow. That is my next test. We are requiring our staff to do so much work that they tend to rush and as I have stated in the past grossing sets the tone for every step the nistotech is responsible for and if it is not prepared correctly in the gross lab the histologist cannot fix it. An old adage we are not magicians. Sue Paturzo TJUH From tony.henwood <@t> health.nsw.gov.au Fri Apr 24 04:12:07 2015 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri Apr 24 04:12:30 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <1471795104.1518386.1429836676486.JavaMail.zimbra@comcast.net> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com>, <1471795104.1518386.1429836676486.JavaMail.zimbra@comcast.net> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EABFB@xmdb04.nch.kids> This seems like a classic case of drying of biopsies prior to fixation. This can occur if biopsies are placed on absorbent paper (or on disinfecting alcohol swabs, heaven forbid). ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sue [suetp918@comcast.net] Sent: Friday, 24 April 2015 10:51 AM To: Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear "Artifact" Hi All So I have been seeing the same issue as I stated in past e-mails. I did one test and fixed a colon biopsy in formaldehyde and left it in overnight and processed next day. I was hoping that I could reproduce the artifact. The tissue was beautiful. At my pathologists requests we changed the paraffin temperature tonight is the first night. I do not think this i the issue. We are going to transfer our biopsies to another tissue processor just for test. I brought up in the past that i think the issue starts prior to the histo lab, my pathologist tended to disagree, but I think he is chaining his mind since my one common detonator is a PA. I do not think that she wets her blue wrap paper enough and the tissue sits on the paper dry she also fold the paper so tight that it is possible for the small biopsies to get stuck in a fold. My pathologists actually came in and said he thought I may be right. Wow. That is my next test. We are requiring our staff to do so much work that they tend to rush and as I have stated in the past grossing sets the tone for every step the nistotech is responsible for and if it is not prepared correctly in the gross lab the histologist cannot fix it. An old adage we are not magicians. Sue Paturzo TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Fri Apr 24 04:43:59 2015 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri Apr 24 04:44:14 2015 Subject: [Histonet] IHC and oven temperature In-Reply-To: References: , Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EAC93@xmdb04.nch.kids> Hi temp drying shown to be a bad idea: Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. Abstract Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac@outlook.com] Sent: Tuesday, 21 April 2015 1:56 AM To: Preiszner, Johanna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and oven temperature Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes Sent from my iPhone > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > Hi Netters, > > is there something wrong with this logic: > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > Thanks! > > Hanna Preiszner > ETSU/QCOM > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From rooki.parak <@t> gmail.com Fri Apr 24 06:05:33 2015 From: rooki.parak <@t> gmail.com (Rooki Parak) Date: Fri Apr 24 06:05:36 2015 Subject: [Histonet] Decalcification using EDTA Message-ID: Hello Histonetters Is it imperative OR optional to maintain samples at below 4 degrees celcius when using EDTA to decalcify bone samples Thank You From mills <@t> 3scan.com Fri Apr 24 09:38:00 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Fri Apr 24 09:38:26 2015 Subject: [Histonet] Leica perfusion 1 or 2 systems Message-ID: Happy Friday Histonet! Does anyone have a perfusion one or two system from Leica that they can tell me how it works, or even better take a little movie? We are testing out different mechanisms of perfusion but do not want to buy one until we know if it is going to work for us! I asked my rep for a demo too. thanks all! mills -- Caroline Miller Director of Histology 3Scan.com 415 2187297 From TNMayer <@t> mdanderson.org Fri Apr 24 09:45:05 2015 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Fri Apr 24 09:45:11 2015 Subject: [Histonet] RE: Histonet Digest, Vol 137, Issue 30 In-Reply-To: <96020995-f51a-4bd4-b958-b3638ad1f062@D1PWPEXHUBCAS05.mdanderson.edu> References: <96020995-f51a-4bd4-b958-b3638ad1f062@D1PWPEXHUBCAS05.mdanderson.edu> Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D56425D@D1PWPEXMBX05.mdanderson.edu> Gail, The regulation is from CLIA '88. They list the requirements for that, and I have never heard of a grandfather clause. I could be wrong about though. NY state did not license HTL. I had a student who wanted to move there, and did not because he was going to have an HTL, not HT. He would only have been able to work clinical if he took both exams (HT and HTL). I even contacted the state society for clarification. Hopefully it has changed. I believe it is because they do not have any HTL programs in the state, so it was not included in the licensure bill. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 8 Date: Thu, 23 Apr 2015 18:34:13 +0000 From: Joelle Weaver Subject: RE: [Histonet] NY State Histology License To: Gail Marcella , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes, CLIA stipulation. I think that there may be a grandfather clause, but not sure of the time frame. You could check the regulation on that. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: gmarcella@nj-urology.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 21 Apr 2015 11:47:58 -0400 > Subject: [Histonet] NY State Histology License > > Hi - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website. I was wondering if anyone else heard of this? Thanks - Gail > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Fri Apr 24 10:06:13 2015 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Fri Apr 24 10:06:17 2015 Subject: [Histonet] RE:Histology in higher education Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D56436C@D1PWPEXMBX05.mdanderson.edu> Thanks for the info. I just signed up. The whole program sounds like a great idea. A few years ago I spoke at my son's middle school about histology and what the job entails. It is challenging to figure out how to reach kids at their level. I may talk about the program in my class next fall to introduce them to outreach in the science fields. Great info. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 11 Date: Wed, 22 Apr 2015 16:24:28 +0000 From: "Piche, Jessica" Subject: RE: [Histonet] histology in higher education To: "'Grantham, Andrea L - (algranth)'" , "\"\"" Message-ID: <631955447A364B45B9458D2905635110D8BF681F@WIN08-MBX-01.wtbyhosp.org> Content-Type: text/plain; charset="us-ascii" Thank you for sharing this information Andi. I'd like to do something like this and I'm going to send this on to my daughters science teachers at school. I think it's a great idea. It always amazed me all the different jobs in hospitals alone that are available for kids and adults alike and no one knows they exist. Especially histology. Looking forward to passing this on! Thanks again, Jessica Piche, HT(ASCP) Waterbury Hospital CT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 12:01 PM To: "" Subject: RE: [Histonet] histology in higher education Bonnie, and anybody who wants to do this: www.prescientist.org ________________________________________ From: Whitaker, Bonnie [Bonnie.Whitaker@osumc.edu] Sent: Wednesday, April 22, 2015 8:45 AM To: Grantham, Andrea L - (algranth); "" Subject: RE: [Histonet] histology in higher education Andi, Would you be willing to share the information on how to volunteer with this program? Thanks, Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 11:40 AM To: "" Subject: RE: [Histonet] histology in higher education For a few years I've been involved in a program called "letters to a pre-scientist". The idea is to reach middle schoolers as they are being introduced to the sciences. They have pretty high goals at this time, they want to be doctors and astronauts and engineers but they are just starting to learn about these things. You become a pen pal/mentor of sorts and write letters to a child and they will write back to you. Last year I was writing to a boy in the Chicago area and this year it was a girl in LA. I always write about what I do and how important it is and include pictures of things like brain cells, muscle, fungus, bacteria and pictures of my lab. I always pick up a copy of the NSH coloring book and send it to them and tell them what they need to study to be a histotech and other than a hospital, where they can find a job. Of course we also tell them about other things like our families, pets, vacations, etc. at the same time. It's just a small thing but it plants a seed. Andi G. From amurvosh <@t> advancederm.net Fri Apr 24 11:03:10 2015 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Fri Apr 24 11:07:35 2015 Subject: [Histonet] RE: NY State Histology License In-Reply-To: <3652170FF5F22544A1280F1DB5D9199411C2D2D4D2@NJUE1.nj-urology.com> References: <3652170FF5F22544A1280F1DB5D9199411C2D2D4D2@NJUE1.nj-urology.com> Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7A82650@Exchange.Advancederm.net> There is a grandfather clause if you were a histotech by april 24.1995. I asked ClIA recently when they came to inspect me, as I fell into this category but didn't know if you had to have the licence by then or just have been performing high complexity test at that time (my licence was later than that). The CLIA person couldn't decipher their own rules and was going to ask her supervisor. I never heard back. As for IHC any HT in our state can do it and CAP never brought it up when I worked at the hospital. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gail Marcella Sent: Tuesday, April 21, 2015 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NY State Histology License Hi - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website. I was wondering if anyone else heard of this? Thanks - Gail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chapmanc <@t> health.missouri.edu Fri Apr 24 11:28:54 2015 From: chapmanc <@t> health.missouri.edu (Chapman, Cherie J.) Date: Fri Apr 24 11:29:00 2015 Subject: [Histonet] RE: Histonet Digest, Vol 137, Issue 30 In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC881D56425D@D1PWPEXMBX05.mdanderson.edu> References: <96020995-f51a-4bd4-b958-b3638ad1f062@D1PWPEXHUBCAS05.mdanderson.edu> <47E9B2C01DDDD94881EACD2DC44EBC881D56425D@D1PWPEXMBX05.mdanderson.edu> Message-ID: FYI- This is what we have listed in our CLIA Testing Personnel Qualifications-High Complexity Testing- Minimum requirements: Earned Associates' degree in laboratory science or medical laboratory technology. (Equivalency: 60 semester hours with adequate course break-down and training experience). Or Prior to April 24, 1995, have graduated from a medical laboratory school that has been approved or have completed a 50-week military training course. High school graduates with appropriate training who are performing high complexity testing on or before April 25, 1995, will continue to qualify. This could be referred to as the grandfather clause. I hope this helps. Cherie Chapman BS, HT, HTL (ASCP) This -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Friday, April 24, 2015 9:45 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Histonet Digest, Vol 137, Issue 30 Gail, The regulation is from CLIA '88. They list the requirements for that, and I have never heard of a grandfather clause. I could be wrong about though. NY state did not license HTL. I had a student who wanted to move there, and did not because he was going to have an HTL, not HT. He would only have been able to work clinical if he took both exams (HT and HTL). I even contacted the state society for clarification. Hopefully it has changed. I believe it is because they do not have any HTL programs in the state, so it was not included in the licensure bill. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 8 Date: Thu, 23 Apr 2015 18:34:13 +0000 From: Joelle Weaver Subject: RE: [Histonet] NY State Histology License To: Gail Marcella , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes, CLIA stipulation. I think that there may be a grandfather clause, but not sure of the time frame. You could check the regulation on that. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: gmarcella@nj-urology.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 21 Apr 2015 11:47:58 -0400 > Subject: [Histonet] NY State Histology License > > Hi - I've been a Histotech for 20+ years and got my Clinical > Laboratory License in NY State when they required getting it. I don't > have an Associates or Bachelor's degree but a Pathologist signed off > for me. I have my HTASCP. I was told when I went for an interview in > NY State that I couldn't gross small specimens or do IHC without an > Associates or Bachelor degree in biology. I was not aware of these > restrictions. I don't see anything on the NYS website. I was > wondering if anyone else heard of this? Thanks - Gail > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah_Mack <@t> urmc.rochester.edu Fri Apr 24 12:09:15 2015 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Fri Apr 24 12:10:56 2015 Subject: [Histonet] RE: Decalcification using EDTA In-Reply-To: <201504241703.t3OH3xQT028613@pps.reinject> References: <201504241703.t3OH3xQT028613@pps.reinject> Message-ID: It is optional to decal at 4 degrees Celsius. We decal most of our bone samples in EDTA at room temperature. Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, April 24, 2015 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 137, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=9GrlE0JWzYNdPAj93-f_VHrRwq-gg31O3ro05gxX1ys&s=5rH0Te-4CBbuQ0YFVCQg1otzKJYnWJX4GncnnPfr7AQ&e= or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Decalcification using EDTA (Rooki Parak) 2. Leica perfusion 1 or 2 systems (Caroline Miller) 3. RE: Histonet Digest, Vol 137, Issue 30 (Mayer,Toysha N) 4. RE:Histology in higher education (Mayer,Toysha N) 5. RE: NY State Histology License (Anne Murvosh) 6. RE: Histonet Digest, Vol 137, Issue 30 (Chapman, Cherie J.) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Apr 2015 13:05:33 +0200 From: Rooki Parak Subject: [Histonet] Decalcification using EDTA To: Histonet Message-ID: Content-Type: text/plain; charset=UTF-8 Hello Histonetters Is it imperative OR optional to maintain samples at below 4 degrees celcius when using EDTA to decalcify bone samples Thank You ------------------------------ Message: 2 Date: Fri, 24 Apr 2015 07:38:00 -0700 From: Caroline Miller Subject: [Histonet] Leica perfusion 1 or 2 systems To: "Histonet@Lists. Edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Happy Friday Histonet! Does anyone have a perfusion one or two system from Leica that they can tell me how it works, or even better take a little movie? We are testing out different mechanisms of perfusion but do not want to buy one until we know if it is going to work for us! I asked my rep for a demo too. thanks all! mills -- Caroline Miller Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 3 Date: Fri, 24 Apr 2015 14:45:05 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE: Histonet Digest, Vol 137, Issue 30 To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D56425D@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Gail, The regulation is from CLIA '88. They list the requirements for that, and I have never heard of a grandfather clause. I could be wrong about though. NY state did not license HTL. I had a student who wanted to move there, and did not because he was going to have an HTL, not HT. He would only have been able to work clinical if he took both exams (HT and HTL). I even contacted the state society for clarification. Hopefully it has changed. I believe it is because they do not have any HTL programs in the state, so it was not included in the licensure bill. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 8 Date: Thu, 23 Apr 2015 18:34:13 +0000 From: Joelle Weaver Subject: RE: [Histonet] NY State Histology License To: Gail Marcella , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes, CLIA stipulation. I think that there may be a grandfather clause, but not sure of the time frame. You could check the regulation on that. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: gmarcella@nj-urology.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 21 Apr 2015 11:47:58 -0400 > Subject: [Histonet] NY State Histology License > > Hi - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website. I was wondering if anyone else heard of this? Thanks - Gail > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=9GrlE0JWzYNdPAj93-f_VHrRwq-gg31O3ro05gxX1ys&s=5rH0Te-4CBbuQ0YFVCQg1otzKJYnWJX4GncnnPfr7AQ&e= ------------------------------ Message: 4 Date: Fri, 24 Apr 2015 15:06:13 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE:Histology in higher education To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D56436C@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="utf-8" Thanks for the info. I just signed up. The whole program sounds like a great idea. A few years ago I spoke at my son's middle school about histology and what the job entails. It is challenging to figure out how to reach kids at their level. I may talk about the program in my class next fall to introduce them to outreach in the science fields. Great info. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 11 Date: Wed, 22 Apr 2015 16:24:28 +0000 From: "Piche, Jessica" Subject: RE: [Histonet] histology in higher education To: "'Grantham, Andrea L - (algranth)'" , "\"\"" Message-ID: <631955447A364B45B9458D2905635110D8BF681F@WIN08-MBX-01.wtbyhosp.org> Content-Type: text/plain; charset="us-ascii" Thank you for sharing this information Andi. I'd like to do something like this and I'm going to send this on to my daughters science teachers at school. I think it's a great idea. It always amazed me all the different jobs in hospitals alone that are available for kids and adults alike and no one knows they exist. Especially histology. Looking forward to passing this on! Thanks again, Jessica Piche, HT(ASCP) Waterbury Hospital CT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 12:01 PM To: "" Subject: RE: [Histonet] histology in higher education Bonnie, and anybody who wants to do this: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.prescientist.org&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=9GrlE0JWzYNdPAj93-f_VHrRwq-gg31O3ro05gxX1ys&s=EufgUSX4U6zECw6kT_EQ9K2y_dxbt-V5A8eIEUojmsI&e= ________________________________________ From: Whitaker, Bonnie [Bonnie.Whitaker@osumc.edu] Sent: Wednesday, April 22, 2015 8:45 AM To: Grantham, Andrea L - (algranth); "" Subject: RE: [Histonet] histology in higher education Andi, Would you be willing to share the information on how to volunteer with this program? Thanks, Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, April 22, 2015 11:40 AM To: "" Subject: RE: [Histonet] histology in higher education For a few years I've been involved in a program called "letters to a pre-scientist". The idea is to reach middle schoolers as they are being introduced to the sciences. They have pretty high goals at this time, they want to be doctors and astronauts and engineers but they are just starting to learn about these things. You become a pen pal/mentor of sorts and write letters to a child and they will write back to you. Last year I was writing to a boy in the Chicago area and this year it was a girl in LA. I always write about what I do and how important it is and include pictures of things like brain cells, muscle, fungus, bacteria and pictures of my lab. I always pick up a copy of the NSH coloring book and send it to them and tell them what they need to study to be a histotech and other than a hospital, where they can find a job. Of course we also tell them about other things like our families, pets, vacations, etc. at the same time. It's just a small thing but it plants a seed. Andi G. ------------------------------ Message: 5 Date: Fri, 24 Apr 2015 16:03:10 +0000 From: Anne Murvosh Subject: [Histonet] RE: NY State Histology License To: Gail Marcella , "histonet@lists.utsouthwestern.edu" Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7A82650@Exchange.Advancederm.net> Content-Type: text/plain; charset="us-ascii" There is a grandfather clause if you were a histotech by april 24.1995. I asked ClIA recently when they came to inspect me, as I fell into this category but didn't know if you had to have the licence by then or just have been performing high complexity test at that time (my licence was later than that). The CLIA person couldn't decipher their own rules and was going to ask her supervisor. I never heard back. As for IHC any HT in our state can do it and CAP never brought it up when I worked at the hospital. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gail Marcella Sent: Tuesday, April 21, 2015 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NY State Histology License Hi - I've been a Histotech for 20+ years and got my Clinical Laboratory License in NY State when they required getting it. I don't have an Associates or Bachelor's degree but a Pathologist signed off for me. I have my HTASCP. I was told when I went for an interview in NY State that I couldn't gross small specimens or do IHC without an Associates or Bachelor degree in biology. I was not aware of these restrictions. I don't see anything on the NYS website. I was wondering if anyone else heard of this? Thanks - Gail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=9GrlE0JWzYNdPAj93-f_VHrRwq-gg31O3ro05gxX1ys&s=5rH0Te-4CBbuQ0YFVCQg1otzKJYnWJX4GncnnPfr7AQ&e= ------------------------------ Message: 6 Date: Fri, 24 Apr 2015 16:28:54 +0000 From: "Chapman, Cherie J." Subject: [Histonet] RE: Histonet Digest, Vol 137, Issue 30 To: "Mayer,Toysha N" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" FYI- This is what we have listed in our CLIA Testing Personnel Qualifications-High Complexity Testing- Minimum requirements: Earned Associates' degree in laboratory science or medical laboratory technology. (Equivalency: 60 semester hours with adequate course break-down and training experience). Or Prior to April 24, 1995, have graduated from a medical laboratory school that has been approved or have completed a 50-week military training course. High school graduates with appropriate training who are performing high complexity testing on or before April 25, 1995, will continue to qualify. This could be referred to as the grandfather clause. I hope this helps. Cherie Chapman BS, HT, HTL (ASCP) This -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Friday, April 24, 2015 9:45 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Histonet Digest, Vol 137, Issue 30 Gail, The regulation is from CLIA '88. They list the requirements for that, and I have never heard of a grandfather clause. I could be wrong about though. NY state did not license HTL. I had a student who wanted to move there, and did not because he was going to have an HTL, not HT. He would only have been able to work clinical if he took both exams (HT and HTL). I even contacted the state society for clarification. Hopefully it has changed. I believe it is because they do not have any HTL programs in the state, so it was not included in the licensure bill. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 8 Date: Thu, 23 Apr 2015 18:34:13 +0000 From: Joelle Weaver Subject: RE: [Histonet] NY State Histology License To: Gail Marcella , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes, CLIA stipulation. I think that there may be a grandfather clause, but not sure of the time frame. You could check the regulation on that. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: gmarcella@nj-urology.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 21 Apr 2015 11:47:58 -0400 > Subject: [Histonet] NY State Histology License > > Hi - I've been a Histotech for 20+ years and got my Clinical > Laboratory License in NY State when they required getting it. I don't > have an Associates or Bachelor's degree but a Pathologist signed off > for me. I have my HTASCP. I was told when I went for an interview in > NY State that I couldn't gross small specimens or do IHC without an > Associates or Bachelor degree in biology. I was not aware of these > restrictions. I don't see anything on the NYS website. I was > wondering if anyone else heard of this? Thanks - Gail > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=9GrlE0JWzYNdPAj93-f_VHrRwq-gg31O3ro05gxX1ys&s=5rH0Te-4CBbuQ0YFVCQg1otzKJYnWJX4GncnnPfr7AQ&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=9GrlE0JWzYNdPAj93-f_VHrRwq-gg31O3ro05gxX1ys&s=5rH0Te-4CBbuQ0YFVCQg1otzKJYnWJX4GncnnPfr7AQ&e= ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=9GrlE0JWzYNdPAj93-f_VHrRwq-gg31O3ro05gxX1ys&s=5rH0Te-4CBbuQ0YFVCQg1otzKJYnWJX4GncnnPfr7AQ&e= End of Histonet Digest, Vol 137, Issue 31 ***************************************** From abtdhu <@t> gmail.com Fri Apr 24 12:51:29 2015 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Fri Apr 24 12:51:37 2015 Subject: [Histonet] Decalcification using EDTA Message-ID: It is optional to decal in 4oC, but it is much better for preserve antigenicity and get good result from enzymatic staining. Similar way as you do fixation, you can choose RT or 4oC. Dorothy Hu Hello Histonetters Is it imperative OR optional to maintain samples at below 4 degrees celcius when using EDTA to decalcify bone samples Thank You From joelleweaver <@t> hotmail.com Fri Apr 24 13:44:34 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Apr 24 13:44:38 2015 Subject: [Histonet] (no subject) Message-ID: its moderate complexity Joelle Weaver MAOM, HTL (ASCP) QIHC From joelleweaver <@t> hotmail.com Fri Apr 24 14:45:29 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Apr 24 14:45:34 2015 Subject: [Histonet] RE: Question on IHC billing In-Reply-To: References: <9B1A1501A800064397369BD8072E6BCAB5C90C@E2K10DB.springfieldclinic.com>, Message-ID: Yes, that is what we do. It is per specimen. First IHC 88342, additional are 88341 Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Joyce.Weems@EmoryHealthcare.org > To: jvickroy@SpringfieldClinic.com; histonet@lists.utsouthwestern.edu > Date: Thu, 23 Apr 2015 20:31:09 +0000 > CC: > Subject: [Histonet] RE: Question on IHC billing > > Correct > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James > Sent: Thursday, April 23, 2015 4:19 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question on IHC billing > > > Let me see if I have this straight: If a pathologist orders an Hpylori stain on 2 blocks from the same specimen C1 and C2 we can only bill one 88342. > > If this correct. Obviously if he ordered addition different IHC stains we could change additional 88341's. > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy@SpringfieldClinic.com > > > > This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you._______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Apr 24 14:49:16 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Apr 24 14:49:19 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157F53EABFB@xmdb04.nch.kids> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com>, , <1471795104.1518386.1429836676486.JavaMail.zimbra@comcast.net>, <6D6BD1DE8A5571489398B392A38A7157F53EABFB@xmdb04.nch.kids> Message-ID: I vote for "wicking" from the papers or other absorbant material. It does not seem like it would affect only sporadic biopsies all processed on the same processor at the same wax temperature. Other artifacts ( sectioning) are histology technique. Other nuclear issues could be staining, but this one doesn't fit that mold. Let us know how the experiment turns out. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood@health.nsw.gov.au > To: suetp918@comcast.net; Royl1@LabCorp.com > Date: Fri, 24 Apr 2015 09:12:07 +0000 > CC: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Nuclear "Artifact" > > This seems like a classic case of drying of biopsies prior to fixation. This can occur if biopsies are placed on absorbent paper (or on disinfecting alcohol swabs, heaven forbid). > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sue [suetp918@comcast.net] > Sent: Friday, 24 April 2015 10:51 AM > To: Lisa Roy > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Nuclear "Artifact" > > Hi All > > So I have been seeing the same issue as I stated in past e-mails. I did one test and fixed a colon biopsy in formaldehyde and left it in overnight and processed next day. I was hoping that I could reproduce the artifact. The tissue was beautiful. At my pathologists requests we changed the paraffin temperature tonight is the first night. I do not think this i the issue. We are going to transfer our biopsies to another tissue processor just for test. I brought up in the past that i think the issue starts prior to the histo lab, my pathologist tended to disagree, but I think he is chaining his mind since my one common detonator is a PA. I do not think that she wets her blue wrap paper enough and the tissue sits on the paper dry she also fold the paper so tight that it is possible for the small biopsies to get stuck in a fold. My pathologists actually came in and said he thought I may be right. Wow. That is my next test. We are requiring our staff to do so much work that they tend to rush and as I have stated in the past grossing sets the tone for every step the nistotech is responsible for and if it is not prepared correctly in the gross lab the histologist cannot fix it. An old adage we are not magicians. > > Sue Paturzo > TJUH > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Apr 24 14:51:14 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Apr 24 14:51:20 2015 Subject: [Histonet] IHC and oven temperature In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157F53EAC93@xmdb04.nch.kids> References: , , , <6D6BD1DE8A5571489398B392A38A7157F53EAC93@xmdb04.nch.kids> Message-ID: I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood@health.nsw.gov.au > To: wdesalvo.cac@outlook.com; PREISZNE@mail.etsu.edu > Date: Fri, 24 Apr 2015 09:43:59 +0000 > Subject: RE: [Histonet] IHC and oven temperature > CC: histonet@lists.utsouthwestern.edu > > Hi temp drying shown to be a bad idea: > > Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. > > Abstract > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac@outlook.com] > Sent: Tuesday, 21 April 2015 1:56 AM > To: Preiszner, Johanna > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC and oven temperature > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes > > Sent from my iPhone > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > > > Hi Netters, > > > > is there something wrong with this logic: > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > Thanks! > > > > Hanna Preiszner > > ETSU/QCOM > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Sat Apr 25 23:25:15 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Apr 25 23:25:19 2015 Subject: [Histonet] Eosin pH In-Reply-To: <72e0fd7bee98.553c68a6@uwo.ca> References: <0d1a8e$1rcbp7@correo2bi.usc.es> <72e0b81cb165.553c6869@uwo.ca> <72e0fd7bee98.553c68a6@uwo.ca> Message-ID: <72e0abb2c3f1.553c225b@uwo.ca> Dissolve it in a buffer (eg acetic acid-sodium acetate) at or near pH 4.5. John Kiernan London, Canada = = = On 23/04/15, Pablo Sanchez-Quinteiro wrote: > Listers, Could you tell me the best way to adjut the eosin pH to 4-5? > > Thanks in advance > > Pablo > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From tkngflght <@t> yahoo.com Sun Apr 26 10:14:50 2015 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sun Apr 26 10:14:56 2015 Subject: [Histonet] Help with PowerPath -- how do you use it? Message-ID: <005a01d08033$beef8500$3cce8f00$@com> Hello All, I need help -- is anyone using PowerPath with the Amp module for barcode and driving slide label production? I am hoping for a 5-10 minute conversation on your workflow, your worksheets, what is working and where you've had to come up with solutions (where it doesn't work as well as you'd hoped). I have to make a couple of decisions for my hospital employer and don't know enough to make them! Call me, write me back on here or in private: My cell 281.883.7704 Email tkngflght@yahoo.com Thank you! Cheryl Kerry From richardboen <@t> charter.net Sun Apr 26 10:42:04 2015 From: richardboen <@t> charter.net (richardboen@charter.net) Date: Sun Apr 26 10:42:08 2015 Subject: [Histonet] Re: IHC and Oven Temperatures Message-ID: <19479f42.2b5195.14cf6648458.Webtop.46@charter.net> The specific pages in the Dako Education Guide: Immunohistochemistry Staining Methods, Fifth Edition are: Discussion on page 32 and references on page 33. It's in the Fixation and Processing Chapter and says no part of the process should have temperatures above 60C. Rick Boen, BS, HTL (ASCP) Histology Lab St. Luke's Hospital Duluth, Mn From joelleweaver <@t> hotmail.com Sun Apr 26 16:27:36 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Sun Apr 26 16:27:40 2015 Subject: [Histonet] Re: IHC and Oven Temperatures In-Reply-To: <19479f42.2b5195.14cf6648458.Webtop.46@charter.net> References: <19479f42.2b5195.14cf6648458.Webtop.46@charter.net> Message-ID: Thank you. I knew it was discussed in that reference. I guess my memory isn't totally gone! Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Sun, 26 Apr 2015 11:42:04 -0400 > From: richardboen@charter.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: IHC and Oven Temperatures > > > The specific pages in the Dako Education Guide: Immunohistochemistry > Staining Methods, Fifth Edition are: Discussion on page 32 and > references on page 33. It's in the Fixation and Processing Chapter and > says no part of the process should have temperatures above 60C. > > Rick Boen, BS, HTL (ASCP) > Histology Lab > St. Luke's Hospital > Duluth, Mn > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Sun Apr 26 18:19:55 2015 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Sun Apr 26 18:20:00 2015 Subject: [Histonet] Re: IHC and Oven Temperatures In-Reply-To: <19479f42.2b5195.14cf6648458.Webtop.46@charter.net> References: <19479f42.2b5195.14cf6648458.Webtop.46@charter.net> Message-ID: <77DD817201982748BC67D7960F2F76AF132398@UWHC-MBX13.uwhis.hosp.wisc.edu> That's the reference I have always gone by since I first started doing IHC back in 19...... Linda A. Sebree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of richardboen@charter.net [richardboen@charter.net] Sent: Sunday, April 26, 2015 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: IHC and Oven Temperatures The specific pages in the Dako Education Guide: Immunohistochemistry Staining Methods, Fifth Edition are: Discussion on page 32 and references on page 33. It's in the Fixation and Processing Chapter and says no part of the process should have temperatures above 60C. Rick Boen, BS, HTL (ASCP) Histology Lab St. Luke's Hospital Duluth, Mn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From taylor <@t> prometheushealthcare.com Mon Apr 27 08:54:36 2015 From: taylor <@t> prometheushealthcare.com (Taylor Rinaldi) Date: Mon Apr 27 08:54:55 2015 Subject: [Histonet] Histology Supervisor and Bench opportunities in Jackson, MS Message-ID: <05c501d080f1$b2fcccd0$18f66670$@prometheushealthcare.com> A growing pathology laboratory in Jackson, MS is looking to hire in their Histology department. Bench and managerial positions available. ASCP certification required. These are permanent, full time opportunities offering relocation assistance. Please reach out to me for immediate consideration. Thanks so much! Taylor Rinaldi Recruiter Prometheus Healthcare Office (301) 693-9057 Taylor@prometheushealthcare.com From patpxs <@t> gmail.com Mon Apr 27 12:35:18 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Mon Apr 27 12:35:22 2015 Subject: [Histonet] COM.40000 (new) Message-ID: Hello Netters, How are you all interpreting this new requirement with regards to multiple autostainers for IHC? Paula :-) From anrecord <@t> saintfrancis.com Mon Apr 27 14:25:29 2015 From: anrecord <@t> saintfrancis.com (Record, Angela N) Date: Mon Apr 27 14:25:38 2015 Subject: [Histonet] need light for flotation bath Message-ID: <20FC170D97CF6546939ABB0FA907C8DB01289A@SFHSEXCH001.WarrenNT.SaintFrancis.Loc> We get ours through our maintenance department in the hospital. The brand is Havells, the code is 5026301 and their website is www.havells-usa.com! Thank You, Angela Record, BS, HT(ASCP) Saint Francis Hospital Tulsa, OK 918-494-1321 ---------------------------------------------------------------------- Saint Francis Health System intends this email only for the use of the person to whom it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you have received this email in error, you are hereby notified that we do not consent to any reading, dissemination, distribution, or copying of this email and request you notify the sender immediately and destroy this transmission. Violators may be prosecuted under Federal law. From anrecord <@t> saintfrancis.com Mon Apr 27 14:35:59 2015 From: anrecord <@t> saintfrancis.com (Record, Angela N) Date: Mon Apr 27 14:36:04 2015 Subject: [Histonet] sta on Message-ID: <20FC170D97CF6546939ABB0FA907C8DB0128A8@SFHSEXCH001.WarrenNT.SaintFrancis.Loc> Pam, What brand of charged slides does your lab use? Thank You, Angela Record, BS, HT(ASCP) Saint Francis Hospital Tulsa, OK 918-494-1321 ---------------------------------------------------------------------- Saint Francis Health System intends this email only for the use of the person to whom it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you have received this email in error, you are hereby notified that we do not consent to any reading, dissemination, distribution, or copying of this email and request you notify the sender immediately and destroy this transmission. Violators may be prosecuted under Federal law. From PAMarcum <@t> uams.edu Mon Apr 27 14:55:48 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Apr 27 14:55:56 2015 Subject: [Histonet] Immediate Opening for HT or HTL in Little Rock AR Message-ID: <3a824afffd0f40248fcf53d991d67fe8@MAIL13M2N2.ad.uams.edu> We are currently looking for a register HT or HTL for full time bench work at the University of Arkansas for Medical Sciences in Little Rock AR. The position will entail processing, embedding and staining for both routine and special stains. The applicant should have experience with computers and Microsoft as we are very automated. The position is for early morning. Please let me know if you are interested. If you are in Little Rock and looking for some PRN work please contact me ASAP. This would also be very early morning only. Recruiters please do not contact me as we cannot use your services due to state regulations. Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jvickroy <@t> SpringfieldClinic.com Mon Apr 27 15:04:30 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Mon Apr 27 15:04:36 2015 Subject: [Histonet] H. Pylori Testing Message-ID: <9B1A1501A800064397369BD8072E6BCAB5E0A1@E2K10DB.springfieldclinic.com> We are a small lab processing mostly GI specimens. Currently we are sending our H. Pylori testing to a local hospital for staining however I can predict that this year we may have around 1000 H. Pylori stains done. I am looking for a small platform or manual test kit to perform the H. Pylori's inhouse. Can someone please give me their thoughts, suggestions, or similar experiences how they have or would approach this endeavor? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From mjones <@t> metropath.com Mon Apr 27 15:11:27 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Apr 27 15:11:37 2015 Subject: [Histonet] H. Pylori Testing Message-ID: We recently switched to IHC stain for HP, however, before that we used Giemsa regressively for those. Not as sensitive as IHC. For IHC we used Cell Marque by hand (with great results) and now we are automated. Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 4/27/15, 2:04 PM, "Vickroy, James" wrote: > >We are a small lab processing mostly GI specimens. Currently we are >sending our H. Pylori testing to a local hospital for staining however I >can predict that this year we may have around 1000 H. Pylori stains done. > I am looking for a small platform or manual test kit to perform the H. >Pylori's inhouse. Can someone please give me their thoughts, >suggestions, or similar experiences how they have or would approach this >endeavor? > >Jim Vickroy >Histology Manager >Springfield Clinic, Main Campus, East Building >1025 South 6th Street >Springfield, Illinois 62703 >Office: 217-528-7541, Ext. 15121 >Email: >jvickroy@SpringfieldClinic.com > > > >This electronic message contains information from Springfield Clinic, LLP >that may be confidential, privileged, and/or sensitive. This information >is intended for the use of the individual(s) or entity(ies) named above. >If you are not the intended recipient, be aware that disclosure, copying, >distribution, or action taken on the contents of this information is >strictly prohibited. If you have received this electronic message in >error, please notify the sender immediately, by electronic mail, so that >arrangements may be made for the retrieval of this electronic message. >Thank you._______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Mon Apr 27 15:27:41 2015 From: mw <@t> personifysearch.com (Matt Ward) Date: Mon Apr 27 15:27:53 2015 Subject: [Histonet] New Technical Training Opportunity Message-ID: <68da4d634148f6e80a68dec321c514ad@mail.gmail.com> Hello Histonet! We have had a world leading client open a new Histology Technical Trainer opportunity in the Chicagoland area. We are targeting histology professionals that are HT or HTL certified to join the team. The opportunity will interact with internal and external customers and has a strong opportunity for growth. Please e-mail me directly at mw@personifysearch.com if you would like to learn more. Thanks! Matt Matt Ward *Program Manager ? RPO* *Personify * 5020 Weston Parkway Suite 315 Cary NC 27513 Direct Line: (919) 459-3654 Toll Free: (800) 875-6188 ext. 103 www.personifysearch.com From garreyf <@t> gmail.com Mon Apr 27 15:49:25 2015 From: garreyf <@t> gmail.com (Garreyf) Date: Mon Apr 27 15:49:30 2015 Subject: [Histonet] H. Pylori Testing In-Reply-To: References: Message-ID: Is it a pain in the neck to do it by hand? I'd like to bring my h pyloris in house as well. I'm trying to create more revenue to support a 2nd histotech. Garrey Sent from my iPhone > On Apr 27, 2015, at 4:11 PM, Michael Ann Jones wrote: > > We recently switched to IHC stain for HP, however, before that we used > Giemsa regressively for those. Not as sensitive as IHC. For IHC we used > Cell Marque by hand (with great results) and now we are automated. > > Michael Ann > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > > > On 4/27/15, 2:04 PM, "Vickroy, James" > wrote: > >> >> We are a small lab processing mostly GI specimens. Currently we are >> sending our H. Pylori testing to a local hospital for staining however I >> can predict that this year we may have around 1000 H. Pylori stains done. >> I am looking for a small platform or manual test kit to perform the H. >> Pylori's inhouse. Can someone please give me their thoughts, >> suggestions, or similar experiences how they have or would approach this >> endeavor? >> >> Jim Vickroy >> Histology Manager >> Springfield Clinic, Main Campus, East Building >> 1025 South 6th Street >> Springfield, Illinois 62703 >> Office: 217-528-7541, Ext. 15121 >> Email: >> jvickroy@SpringfieldClinic.com >> >> >> >> This electronic message contains information from Springfield Clinic, LLP >> that may be confidential, privileged, and/or sensitive. This information >> is intended for the use of the individual(s) or entity(ies) named above. >> If you are not the intended recipient, be aware that disclosure, copying, >> distribution, or action taken on the contents of this information is >> strictly prohibited. If you have received this electronic message in >> error, please notify the sender immediately, by electronic mail, so that >> arrangements may be made for the retrieval of this electronic message. >> Thank you._______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Mon Apr 27 16:11:54 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Mon Apr 27 16:11:57 2015 Subject: [Histonet] A question about a new CAP requirement Message-ID: Hello Netters, How are you all interpreting this new requirement (COM.40000) with regards to multiple autostainers for IHC? Thank you in advance, Paula :-) From mjones <@t> metropath.com Mon Apr 27 16:15:23 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Apr 27 16:15:28 2015 Subject: [Histonet] H. Pylori Testing In-Reply-To: References: Message-ID: If you do it by IHC it is the normal 2 hour or so process step-by-step. Giemsa is quick 15 minutes or around there? Again, not as sensitive but works. Michael Ann On 4/27/15, 2:49 PM, "Garreyf" wrote: >Is it a pain in the neck to do it by hand? I'd like to bring my h >pyloris in house as well. I'm trying to create more revenue to support a >2nd histotech. > >Garrey > >Sent from my iPhone > >> On Apr 27, 2015, at 4:11 PM, Michael Ann Jones >>wrote: >> >> We recently switched to IHC stain for HP, however, before that we used >> Giemsa regressively for those. Not as sensitive as IHC. For IHC we used >> Cell Marque by hand (with great results) and now we are automated. >> >> Michael Ann >> Michael Ann Jones, HT (ASCP) >> Histology Manager >> Metropath >> 7444 W. Alaska Dr. #250 >> Lakewood, CO 80226 >> 303.634.2511 >> Mjones@metropath.com >> >> >> >> >> >> >> On 4/27/15, 2:04 PM, "Vickroy, James" >> wrote: >> >>> >>> We are a small lab processing mostly GI specimens. Currently we are >>> sending our H. Pylori testing to a local hospital for staining however >>>I >>> can predict that this year we may have around 1000 H. Pylori stains >>>done. >>> I am looking for a small platform or manual test kit to perform the H. >>> Pylori's inhouse. Can someone please give me their thoughts, >>> suggestions, or similar experiences how they have or would approach >>>this >>> endeavor? >>> >>> Jim Vickroy >>> Histology Manager >>> Springfield Clinic, Main Campus, East Building >>> 1025 South 6th Street >>> Springfield, Illinois 62703 >>> Office: 217-528-7541, Ext. 15121 >>> Email: >>> jvickroy@SpringfieldClinic.com >>> >>> >>> >>> This electronic message contains information from Springfield Clinic, >>>LLP >>> that may be confidential, privileged, and/or sensitive. This >>>information >>> is intended for the use of the individual(s) or entity(ies) named >>>above. >>> If you are not the intended recipient, be aware that disclosure, >>>copying, >>> distribution, or action taken on the contents of this information is >>> strictly prohibited. If you have received this electronic message in >>> error, please notify the sender immediately, by electronic mail, so >>>that >>> arrangements may be made for the retrieval of this electronic message. >>> Thank you._______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> sanfordburnham.org Mon Apr 27 21:19:02 2015 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Mon Apr 27 21:19:09 2015 Subject: [Histonet] Florida Society for Histotechnology Meeting Message-ID: Good Morning Histonetters! Final hotel reservations should be made no later than April 30rd, 2015 to ensure rate and availability. After today you will have to hope that room prices will not go up. I am working on trying to squeeze out a few more days for the hotel registration link below. Try this link below first and if it does not work make sure that when you call (1-866-397-6516) the hotel to make reservations that you mention Florida Society for Histotechnology. Act now to save money and possible aggravation. Looking forward to seeing you at our meeting! Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402&hotelID=6579 Need to book no later than 4-30-15 Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155&lbrd=1&rtypeid=380141 Have a great day! Kind Regards! John Shelley 2014-2016 FSH President From brendal.finlay <@t> medicalcenterclinic.com Tue Apr 28 07:25:36 2015 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Tue Apr 28 07:26:42 2015 Subject: [Histonet] H. Pylori Testing References: Message-ID: We do HP and Mart-1 testing in house by hand using concentrates.? It's a pretty basic set up using a pressure cooker, stain tray, pap pen, and the reagents.?At this point in time it's less expensive than automation.? We start them around 10 am and finish around 12 or 1 pm.? ? Brendal C. Finlay, HT (ASCP) Senior Histologist Medical Center Clinic 8333 North Davis Highway Pensacola, FL 32514 Phone 850.474.8581 Fax 850.474.8584? -----Original Message----- From: Garreyf To: "Michael Ann Jones" Cc: histonet@lists.utsouthwestern.edu, "Vickroy, James" Date: 04/27/2015 15:49 Subject: Re: [Histonet] H. Pylori Testing Is it a pain in the neck to do it ?by hand? I'd like to bring my h pyloris in house as well. I'm trying to create more revenue to support a 2nd histotech. Garrey Sent from my iPhone > On Apr 27, 2015, at 4:11 PM, Michael Ann Jones wrote: > > We recently switched to IHC stain for HP, however, before that we used > Giemsa regressively for those. Not as sensitive as IHC. For IHC we used > Cell Marque by hand (with great results) and now we are automated. > > Michael Ann > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > > > On 4/27/15, 2:04 PM, "Vickroy, James" > wrote: > >> >> We are a small lab processing mostly GI specimens. ?Currently we are >> sending our H. Pylori testing to a local hospital for staining however I >> can predict that this year we may have around 1000 H. Pylori stains done. >> I am looking for a small platform or manual test kit to perform the H. >> Pylori's inhouse. ? Can someone please give me their thoughts, >> suggestions, or similar experiences ?how they have or would approach this >> endeavor? >> >> Jim Vickroy >> Histology Manager >> Springfield Clinic, Main Campus, East Building >> 1025 South 6th Street >> Springfield, Illinois ?62703 >> Office: ?217-528-7541, Ext. 15121 >> Email: ? >> jvickroy@SpringfieldClinic.com >> >> >> >> This electronic message contains information from Springfield Clinic, LLP >> that may be confidential, privileged, and/or sensitive. This information >> is intended for the use of the individual(s) or entity(ies) named above. >> If you are not the intended recipient, be aware that disclosure, copying, >> distribution, or action taken on the contents of this information is >> strictly prohibited. If you have received this electronic message in >> error, please notify the sender immediately, by electronic mail, so that >> arrangements may be made for the retrieval of this electronic message. >> Thank you._______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab.hymclab <@t> ministryhealth.org Tue Apr 28 10:09:58 2015 From: hymclab.hymclab <@t> ministryhealth.org (WIAPP-MB-hymclab) Date: Tue Apr 28 10:11:40 2015 Subject: [Histonet] RE: Tri-State Histology Symposium In-Reply-To: References: <43963a03b96143e0ad30730d9d52461c@PHUSCB-SP37MB04.genoptix.org> Message-ID: Last chance to get on the bandwagon and join our Tri-State histology meeting next week!!!! It promises to be a great meeting!! Go to www.whs.wildapricot.org to sign up or see full brochure. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Monday, March 02, 2015 11:07 AM To: 'Teri Johnson'; Colleen_Herring@bshsi.org Cc: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tri-State Histology Symposium Dear Histonetters: You are invited to join the histology societies of Wisconsin, Iowa and Minnesota as we celebrate "Hats Off to Histology" at the 2015 Tri-State Histology Symposium, May 6-8 at The Madison Concourse Hotel and Governors Club in Madison, Wisconsin. For program, registration and vendor/exhibit information contact the following representatives: Wisconsin: Kathryn Stoll kstoll@mcw.edu Iowa: Judi Stasko judith.stasko@ars.usda.gov Minnesota: Lois Rowe rowe.lois@mayo.edu Vendor/Exhibit: Dawn Schneider dawn.schneider@ministryhealth.org ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From ltougas <@t> dawsoncollege.qc.ca Tue Apr 28 10:23:27 2015 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Tue Apr 28 10:23:34 2015 Subject: [Histonet] URGENT: microwave oven for staining and antigen retrieval Message-ID: <455897B94CF3A44F81F727160F23FB11640C203B@DC229.ad.dawsoncollege.qc.ca> Hi all, We need to purchase a vented laboratory microwave oven that will be used only for staining, secondary fixation (Bouin) and antigen retrieval (no processing, drying of slides or routine fixation). It will be used only during 2 months of the year in the histotechniques course. However, during the days it will be used, as students are going to be using it one after the other, ventilation has to be efficient (hooked up to central system). I would appreciate any advice as to brand and model suggested ASAP. Thanks so much in advance, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal, Qc From jpiche <@t> wtbyhosp.org Tue Apr 28 10:58:07 2015 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Tue Apr 28 10:58:12 2015 Subject: [Histonet] Centrifuge for cytospins Message-ID: <631955447A364B45B9458D2905635110D8C1E075@WIN08-MBX-01.wtbyhosp.org> Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From bcooper <@t> chla.usc.edu Tue Apr 28 10:59:55 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Apr 28 11:00:01 2015 Subject: [Histonet] Adenovirus controls Message-ID: Good morning Histonet, Would any of you have any adenovirus control blocks you'd be willing to trade? We have real fungus and gram control blocks we can part with (not orange peels or Slim Jims :)). Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From tmcampbe <@t> fmh.org Tue Apr 28 11:05:16 2015 From: tmcampbe <@t> fmh.org (Campbell, Tasha M.) Date: Tue Apr 28 11:05:25 2015 Subject: [Histonet] new labeling system Message-ID: Hello all, Does anyone know what we in the lab need to do for this new global labeling system for chemicals? Do we just need to get updated safety sheets from manufacturers for all chemicals in the lab? Do we need to change how anything is labeled in the lab? Any information is appreciated! Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From bcooper <@t> chla.usc.edu Tue Apr 28 11:09:37 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Apr 28 11:09:43 2015 Subject: [Histonet] RE: Centrifuge for cytospins In-Reply-To: <631955447A364B45B9458D2905635110D8C1E075@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110D8C1E075@WIN08-MBX-01.wtbyhosp.org> Message-ID: We make our BAL cytospins directly--no centrifuging first. Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 28, 2015 8:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Centrifuge for cytospins Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From mbireir <@t> yahoo.com Tue Apr 28 11:11:05 2015 From: mbireir <@t> yahoo.com (Manahil) Date: Tue Apr 28 11:11:15 2015 Subject: [Histonet] Fixation of frozen section Message-ID: <1628C165-8B56-47B1-8B10-0D7AA10F44B0@yahoo.com> Hi histonet, Would like to query about which fixative do you prefer for fresh frozen section slide before H&E staining? Thanks for your advices Manahil HTL/ ASCP Sent from my iPhone From GKeyser <@t> uwhealth.org Tue Apr 28 11:16:17 2015 From: GKeyser <@t> uwhealth.org (Keyser Gerald T) Date: Tue Apr 28 11:16:22 2015 Subject: [Histonet] Fixation of frozen section In-Reply-To: <1628C165-8B56-47B1-8B10-0D7AA10F44B0@yahoo.com> References: <1628C165-8B56-47B1-8B10-0D7AA10F44B0@yahoo.com> Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE053CC4@UWHC-MBX13.uwhis.hosp.wisc.edu> Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin. But, it's effective and far less toxic than formalin. Gerry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Manahil Sent: Tuesday, April 28, 2015 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation of frozen section Hi histonet, Would like to query about which fixative do you prefer for fresh frozen section slide before H&E staining? Thanks for your advices Manahil HTL/ ASCP Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbireir <@t> yahoo.com Tue Apr 28 11:23:29 2015 From: mbireir <@t> yahoo.com (Manahil) Date: Tue Apr 28 11:23:36 2015 Subject: [Histonet] Fixation of frozen section In-Reply-To: <5226C88C65EBFF4BAD552D68DC6E8FFE053CC4@UWHC-MBX13.uwhis.hosp.wisc.edu> References: <1628C165-8B56-47B1-8B10-0D7AA10F44B0@yahoo.com> <5226C88C65EBFF4BAD552D68DC6E8FFE053CC4@UWHC-MBX13.uwhis.hosp.wisc.edu> Message-ID: <7B4EF88D-99C9-42A4-96BB-0CAE91CFF0C2@yahoo.com> What about the Absolute Methanol? Sent from my iPhone > On Apr 28, 2015, at 8:16 PM, Keyser Gerald T wrote: > > Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin. But, it's effective and far less toxic than formalin. > > Gerry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Manahil > Sent: Tuesday, April 28, 2015 11:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Fixation of frozen section > > Hi histonet, > > Would like to query about which fixative do you prefer for fresh frozen section slide before H&E staining? > Thanks for your advices > Manahil > HTL/ ASCP > > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Apr 28 11:33:21 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Apr 28 11:33:26 2015 Subject: [Histonet] Re: H. Pylori Testing Message-ID: >From the pathologist's viewpoint, immunohistochemical stains for Helicobacter are much faster to read than are the old dye methods such as Giemsa. Ventana claims that you can break even with one of their stainers if you produce 600 billable slides a year (unless they've changed that number - a rep can tell you. It's likely that we are going to get a lot more resistance in the future from the third parties about paying for a Helicobacter stain done in advance of the pathologist's looking at the slide. I'd think about that before investing very much in anything. Bob Richmond Samurai Pathologist Maryville TN From rsrichmond <@t> gmail.com Tue Apr 28 11:35:17 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Apr 28 11:35:21 2015 Subject: [Histonet] Re: Fixation of frozen section Message-ID: Fixation of frozen sections in surgical pathology: about the only purpose of the fixation step is to protect the hematoxylin from contamination. Alcohol works fine for this purpose. Bob Richmond Samurai Pathologist Maryville TN From j.rowaihi <@t> alborglaboratories.com Tue Apr 28 12:07:07 2015 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Tue Apr 28 12:07:26 2015 Subject: [Histonet] Fixation of frozen section In-Reply-To: <7B4EF88D-99C9-42A4-96BB-0CAE91CFF0C2@yahoo.com> References: <1628C165-8B56-47B1-8B10-0D7AA10F44B0@yahoo.com> <5226C88C65EBFF4BAD552D68DC6E8FFE053CC4@UWHC-MBX13.uwhis.hosp.wisc.edu> <7B4EF88D-99C9-42A4-96BB-0CAE91CFF0C2@yahoo.com> Message-ID: <013f01d081d5$c8ac0c00$5a042400$@rowaihi@alborglaboratories.com> You can't use full concentrate alcohol on such specimen, otherwise you will destroy the cells membrane. For me I am using 80% Ethanol. Best Regards, Jamal Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Manahil Sent: Tuesday, April 28, 2015 7:23 PM To: Keyser Gerald T Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fixation of frozen section What about the Absolute Methanol? Sent from my iPhone > On Apr 28, 2015, at 8:16 PM, Keyser Gerald T wrote: > > Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin. But, it's effective and far less toxic than formalin. > > Gerry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Manahil > Sent: Tuesday, April 28, 2015 11:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Fixation of frozen section > > Hi histonet, > > Would like to query about which fixative do you prefer for fresh frozen section slide before H&E staining? > Thanks for your advices > Manahil > HTL/ ASCP > > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GKeyser <@t> uwhealth.org Tue Apr 28 12:26:01 2015 From: GKeyser <@t> uwhealth.org (Keyser Gerald T) Date: Tue Apr 28 12:26:10 2015 Subject: [Histonet] Fixation of frozen section In-Reply-To: <013f01d081d5$c8ac0c00$5a042400$@rowaihi@alborglaboratories.com> References: <1628C165-8B56-47B1-8B10-0D7AA10F44B0@yahoo.com> <5226C88C65EBFF4BAD552D68DC6E8FFE053CC4@UWHC-MBX13.uwhis.hosp.wisc.edu> <7B4EF88D-99C9-42A4-96BB-0CAE91CFF0C2@yahoo.com> <013f01d081d5$c8ac0c00$5a042400$@rowaihi@alborglaboratories.com> Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE053CEB@UWHC-MBX13.uwhis.hosp.wisc.edu> We get good results with 95%. Unless prepping sample for Electron Microscopy, cell membrane preservation doesn't seem to be worth worrying about. It can't be resolved with a light microscope. A light microscope using a violet monochromatic light source should have the theoretical limits of resolution of approx. 200nm. Cell membranes... what do they run... approx. 4nm? Gerry -----Original Message----- From: Jamal [mailto:j.rowaihi@alborglaboratories.com] Sent: Tuesday, April 28, 2015 12:07 PM To: 'Manahil'; Keyser Gerald T Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Fixation of frozen section You can't use full concentrate alcohol on such specimen, otherwise you will destroy the cells membrane. For me I am using 80% Ethanol. Best Regards, Jamal Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Manahil Sent: Tuesday, April 28, 2015 7:23 PM To: Keyser Gerald T Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fixation of frozen section What about the Absolute Methanol? Sent from my iPhone > On Apr 28, 2015, at 8:16 PM, Keyser Gerald T wrote: > > Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin. But, it's effective and far less toxic than formalin. > > Gerry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Manahil > Sent: Tuesday, April 28, 2015 11:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Fixation of frozen section > > Hi histonet, > > Would like to query about which fixative do you prefer for fresh > frozen section slide before H&E staining? > Thanks for your advices > Manahil > HTL/ ASCP > > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joewalker <@t> rrmc.org Tue Apr 28 13:31:18 2015 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Apr 28 13:31:24 2015 Subject: [Histonet] RE: Centrifuge for cytospins Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1D8ECEAE@RRMBX03.rrmc.local> In all of my experiences with cytology preparations, the first step would be to centrifuge the specimen. This concentrates the cells for the next step; cytocentrifuge, which is used for the presentation of the cells for evaluation. Here is an excerpt from our procedure: 3. Label a 50 ml centrifuge tube with the cytology accession label. 4. Mix the specimen using a vortex mixer for 10-30 seconds. 5. Pour contents of specimen from the original container into the 50 ml centrifuge tube and cap the tube. 6. Centrifuge the specimen at 600g for 10 minutes (program 1) or 1200g for 5 minutes (program 2). 7. Pour off the supernatant and re-suspend the sediment in 0.5 to 1ml of Cytospin (green) fluid, depending on the cell button size. Evaluating the number of drops you might need for your cytocentrifuge can be performed by taking a crop from the sediment, placing it on a slide, placing a 24x50mm cover glass on the specimen and then observing the cellularity of the specimen. I have document that illustrates this but I don't think the list serve will allow attachments. You can email me privately for this document. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Tuesday, April 28, 2015 12:10 PM To: Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Centrifuge for cytospins We make our BAL cytospins directly--no centrifuging first. Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 28, 2015 8:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Centrifuge for cytospins Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From Nancy.Stedman <@t> buschgardens.com Tue Apr 28 13:53:30 2015 From: Nancy.Stedman <@t> buschgardens.com (Stedman, Nancy) Date: Tue Apr 28 13:54:02 2015 Subject: [Histonet] Re: H. Pylori Testing In-Reply-To: References: Message-ID: <18D791D4EE07BC41BF05F8EF3CCCDE246792E46F@FTCSEAP4001.nam.int.local> I believe IHC is more sensitive than the special stains too. One caveat for anyone who works with veterinary samples - the H. pylori antibodies are specific for H. pylori, so I have not found these antibodies to be helpful for evaluating other species with helicobacter-associated gastritis. One exception is the antibody made by Biocare which seems to stain some of the feline helicobacters, and maybe others too (have not tried). -Nancy Stedman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Tuesday, April 28, 2015 12:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H. Pylori Testing >From the pathologist's viewpoint, immunohistochemical stains for Helicobacter are much faster to read than are the old dye methods such as Giemsa. Ventana claims that you can break even with one of their stainers if you produce 600 billable slides a year (unless they've changed that number - a rep can tell you. It's likely that we are going to get a lot more resistance in the future from the third parties about paying for a Helicobacter stain done in advance of the pathologist's looking at the slide. I'd think about that before investing very much in anything. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Albert.Santiago <@t> uphs.upenn.edu Tue Apr 28 13:56:51 2015 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Tue Apr 28 13:57:01 2015 Subject: [Histonet] Negative tissue controls for Special stains Message-ID: Hello fellow Histonetters, I was wondering if anyone uses any specific type of tissue as a negative control for some or most special stains? Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago@uphs.upenn.edu From ihcworkshop <@t> gmail.com Tue Apr 28 13:59:53 2015 From: ihcworkshop <@t> gmail.com (IHC Workshop) Date: Tue Apr 28 13:59:56 2015 Subject: [Histonet] 12 CEU credits for IHC wet workshop Message-ID: Learn IHC techniques in this 2-day hands-on IHC wet workshop in Northern California. Workshop is approved for 12 contact hours. For more info and registration http://innvx.com/workshop.html From patrick.lewis <@t> seattlechildrens.org Tue Apr 28 16:55:43 2015 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Tue Apr 28 16:55:49 2015 Subject: [Histonet] Acetone fixation problems with OCT Tissues Message-ID: <3903BE18914F4440834F0E620415FFCA3CB67012@PPWEXD01d.childrens.sea.kids> Hi Everyone, I am still having issues with my IHCs with Acetone fixation. If I fix in 100% Acetone, I get IHC staining, but my tissues are 50-90% destroyed. If I fix in 4% paraformaldehyde, or 10% NBF or (95% Etoh and/or Methanol with Acetone) I lose the epitopes I either get no staining or very weak staining, but the tissue morphology look fine. I just tried an acetone gradient where I cut the tissues at 5 uM and dried them overnight, then fixed for 10 minutes in 100% acetone, then fixed in 95% acetone for 1 minute, then fixed in 70% acetone for 30 seconds, then quick rinsed in H20, then washed as normal in DPBS pH 7.4. I did 4 slides, 2 slides with one company's Charged slides ,and 2 slides with another company's charged slides. One company's slides look completely destroyed, the others may turn out, it was hard to tell how much damage there was. I'll know tomorrow when I finish staining and Hemotoxylin them. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From clmcmah <@t> clemson.edu Tue Apr 28 17:25:54 2015 From: clmcmah <@t> clemson.edu (Chad L McMahan) Date: Tue Apr 28 17:25:57 2015 Subject: [Histonet] 2015 SCSHT Fall meeting Histo-expo Message-ID: <1DA1B16C-2C70-4E37-93E7-D31ADC8C470C@clemson.edu> We are excited to announce the South Carolina Society for Histotechnology is offering a Fall 2015 meeting. We have a great event planned with speakers of the trade, vendor golf tournament, and much more! The event is for histo-everyones from Region III territory and abroad. Please contact Chad McMahan at clmcmah@clemson.edu for further details. SCSHT 2015 Fall meeting October 23-25, 2015 Litchfield Beach and Golf Resort Pawleys Island, SC Hope to see you there! Regards, Chad McMahan Sent from my iPhone From pwnoyce <@t> gmail.com Wed Apr 29 02:22:53 2015 From: pwnoyce <@t> gmail.com (Peter Noyce) Date: Wed Apr 29 02:20:43 2015 Subject: [Histonet] plant shoot apical meristem preparation for flow cytometry AND confocal scanning laser microscopy Message-ID: <001401d0824d$50fc2960$f2f47c20$@gmail.com> We wish to measure amount of DNA in grapevine bud shoot apical meristem (small 1 by 1mm)-any advice or modern references as to fixation, permeabilization, best stain (proabably propridium iodide). Regards Peter Noyce. From j.benavides <@t> eae.csic.es Wed Apr 29 02:38:17 2015 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Wed Apr 29 02:38:29 2015 Subject: [Histonet] Acetone fixation problems with OCT Tissues In-Reply-To: <3903BE18914F4440834F0E620415FFCA3CB67012@PPWEXD01d.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA3CB67012@PPWEXD01d.childrens.sea.kids> Message-ID: <20150429093817.Horde.eDc01_Hhm7TDVcTEGYqaXw5@webmail.csic.es> To dry them overnight before fixation is not a bit too risky? I ?mean, wouldn?t the tissue degradate? I normaly fixed the section as soon as I get it into the slide. Just a thought. I am no expert in cryostate, just an occasional user. Julio "Lewis, Patrick" escribi?: > Hi Everyone, > > I am still having issues with my IHCs with Acetone fixation. > > If I fix in 100% Acetone, I get IHC staining, but my tissues are 50-90% > destroyed. > > If I fix in 4% paraformaldehyde, or 10% NBF or (95%? Etoh and/or > Methanol with Acetone) I lose the epitopes I either get no staining or > very? weak staining, but the tissue morphology look fine. > > I just tried an acetone gradient where I cut the tissues at 5 uM and > dried them overnight, then fixed for 10 minutes in 100% acetone, then > fixed in 95% acetone for 1 minute, then fixed in 70% acetone for 30 > seconds, then quick rinsed in H20, then washed as normal in DPBS pH 7.4. > > I did 4 slides, 2 slides with one company's Charged slides ,and 2 slides > with another company's charged slides. > > One company's slides look completely destroyed, the others may turn out, > it was hard to tell how much damage there was.? I'll know tomorrow when > I finish staining and Hemotoxylin them. > > Patrick Lewis > Research Associate II Bench > Seattle Childrens Research Institute > 206-884-1115 > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information protected by law. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by reply > e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Wed Apr 29 08:08:10 2015 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Apr 29 08:08:26 2015 Subject: [Histonet] RE: Acetone fixation problems with OCT Tissues In-Reply-To: <3903BE18914F4440834F0E620415FFCA3CB67012@PPWEXD01d.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA3CB67012@PPWEXD01d.childrens.sea.kids> Message-ID: Patrick, We do a lot of frozen section IHC work. Years ago Gayle Callis turned me on to fixing in cold acetone:ethanol (3:1) . We keep it at -20C and I fix for 10 min. on the bench then wash in PBS and proceed with the IHC. We do dry slides for at least 30 min before fixing. This has worked well in our hands for many different antibodies. Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Tuesday, April 28, 2015 5:56 PM To: (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] Acetone fixation problems with OCT Tissues Hi Everyone, I am still having issues with my IHCs with Acetone fixation. If I fix in 100% Acetone, I get IHC staining, but my tissues are 50-90% destroyed. If I fix in 4% paraformaldehyde, or 10% NBF or (95% Etoh and/or Methanol with Acetone) I lose the epitopes I either get no staining or very weak staining, but the tissue morphology look fine. I just tried an acetone gradient where I cut the tissues at 5 uM and dried them overnight, then fixed for 10 minutes in 100% acetone, then fixed in 95% acetone for 1 minute, then fixed in 70% acetone for 30 seconds, then quick rinsed in H20, then washed as normal in DPBS pH 7.4. I did 4 slides, 2 slides with one company's Charged slides ,and 2 slides with another company's charged slides. One company's slides look completely destroyed, the others may turn out, it was hard to tell how much damage there was. I'll know tomorrow when I finish staining and Hemotoxylin them. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lcolbert <@t> pathmdlabs.com Wed Apr 29 08:54:11 2015 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Apr 29 08:54:19 2015 Subject: [Histonet] Over- decalcified tissue Message-ID: <12ECD7346266D74691EC2BFC75285E455B26355C@BFL323E10.pathmdlabs.local> I have tissue that was left in decal over the weekend and now has very poor nuclear staining. Is there a "fix" for this so that I can get better nuclear staining (other than restaining for a long time in the hematoxylin)? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From ltougas <@t> dawsoncollege.qc.ca Wed Apr 29 09:54:40 2015 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Wed Apr 29 09:54:50 2015 Subject: [Histonet] RE: URGENT: microwave oven for staining and antigen retrieval In-Reply-To: <455897B94CF3A44F81F727160F23FB11640C203B@DC229.ad.dawsoncollege.qc.ca> References: <455897B94CF3A44F81F727160F23FB11640C203B@DC229.ad.dawsoncollege.qc.ca> Message-ID: <455897B94CF3A44F81F727160F23FB11640C22C5@DC229.ad.dawsoncollege.qc.ca> Thank you so much you to the ones who already replied to me! However I did not receive any comment about the H1850 or H2250 from Energy Beam Science (EBS). Does anyone have experience with any of these? Thanks again in advance for a most appreciated prompt reply! Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College 514-931-8731, ext 1519 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Liette Tougas [ltougas@dawsoncollege.qc.ca] Sent: April 28, 2015 11:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] URGENT: microwave oven for staining and antigen retrieval Hi all, We need to purchase a vented laboratory microwave oven that will be used only for staining, secondary fixation (Bouin) and antigen retrieval (no processing, drying of slides or routine fixation). It will be used only during 2 months of the year in the histotechniques course. However, during the days it will be used, as students are going to be using it one after the other, ventilation has to be efficient (hooked up to central system). I would appreciate any advice as to brand and model suggested ASAP. Thanks so much in advance, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal, Qc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgflem <@t> gmail.com Wed Apr 29 10:58:29 2015 From: mgflem <@t> gmail.com (Matthew Fleming) Date: Wed Apr 29 10:58:33 2015 Subject: [Histonet] Part-time histotech needed in Milwaukee Message-ID: Part-time histotech needed for an independent dermatopathology lab in Milwaukee, WI. This an approximately half-time position, though the hours are somewhat flexible and negotiable. Good working environment and highly competitive compensation. If interested, please contact the lab director by email: Matthew Fleming, MD mgflem@gmail.com From pablo.sanchez <@t> usc.es Wed Apr 29 11:07:41 2015 From: pablo.sanchez <@t> usc.es (pablo.sanchez@usc.es) Date: Wed Apr 29 11:07:47 2015 Subject: [Histonet] Over- decalcified tissue In-Reply-To: <12ECD7346266D74691EC2BFC75285E455B26355C@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E455B26355C@BFL323E10.pathmdlabs.local> Message-ID: <20150429180741.30321tpr6tafai9w@correoweb.usc.es> As I usually process gross pieces of bone -that need abouth thirty hours decalcifying- I suffer the same nuissance. Also would thank any hint. Pablo Sanchez Laurie Colbert escribiu: > I have tissue that was left in decal over the weekend and now has > very poor nuclear staining. Is there a "fix" for this so that I can > get better nuclear staining (other than restaining for a long time > in the hematoxylin)? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain > privileged and confidential information, including patient > information protected by federal and state privacy laws. It is > intended only for the use of the person(s) named above. If you are > not the intended recipient, you are hereby notified that any review, > dissemination, distribution, or duplication of this communication is > strictly prohibited. If you are not the intended recipient, please > contact the sender by reply email and destroy all copies of the > original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bburnett <@t> CapeCodHealth.org Wed Apr 29 11:13:16 2015 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Wed Apr 29 11:13:31 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com>, <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> Message-ID: We are having similar issues with our tissue. Any troubleshooting insight would be greatly appreciated! Thanks! ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sue [suetp918@comcast.net] Sent: Tuesday, April 21, 2015 7:55 PM To: Lisa Roy Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear "Artifact" OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. Susan T. Paturzo TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From wdesalvo.cac <@t> outlook.com Wed Apr 29 11:20:48 2015 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Wed Apr 29 11:20:57 2015 Subject: [Histonet] Over- decalcified tissue In-Reply-To: <20150429180741.30321tpr6tafai9w@correoweb.usc.es> References: <12ECD7346266D74691EC2BFC75285E455B26355C@BFL323E10.pathmdlabs.local> <20150429180741.30321tpr6tafai9w@correoweb.usc.es> Message-ID: I would suggest not allowing decalcification to be extended. Better to leave in fixative until you can control the time of endpoint of decalcification. What type of decal solution are you using? I am not a big fan of trying to adjust the chemistry of the stain to compensate for over decalcification. Is there opportunity to submit another sample? Sent from my iPhone > On Apr 29, 2015, at 9:08 AM, pablo.sanchez@usc.es wrote: > > As I usually process gross pieces of bone -that need abouth thirty hours decalcifying- I suffer the same nuissance. Also would thank any hint. > > Pablo Sanchez > > > > Laurie Colbert escribiu: > >> I have tissue that was left in decal over the weekend and now has very poor nuclear staining. Is there a "fix" for this so that I can get better nuclear staining (other than restaining for a long time in the hematoxylin)? >> >> Laurie Colbert, HT (ASCP) >> Histology Supervisor >> PATH MD >> 8158 Beverly Blvd. >> Los Angeles, CA 90048 >> (323) 648-3214 direct >> (424) 245-7284 main lab >> >> The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Wed Apr 29 11:27:54 2015 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Wed Apr 29 11:28:04 2015 Subject: [Histonet] RE: Nuclear "Artifact" In-Reply-To: References: <1556E1132223E046BC888693B2B4A27C04BE10@rtwedb03.lca.labcorp.com> <1465858785.1249339.1429660522076.JavaMail.zimbra@comcast.net> Message-ID: What type of tissue cassette is being used? What type of insert or wrap is used. If one cassette processes correctly and the next to it does not, hard to say tissue processor is causing issue. Sounds like a water problem and it could be water trapped in cassette. Check the rest of you process before moving to the processor. Sent from my iPhone > On Apr 29, 2015, at 9:13 AM, Burnett, Brandy wrote: > > We are having similar issues with our tissue. > Any troubleshooting insight would be greatly appreciated! > Thanks! > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sue [suetp918@comcast.net] > Sent: Tuesday, April 21, 2015 7:55 PM > To: Lisa Roy > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Nuclear "Artifact" > > OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. > > Susan T. Paturzo > TJUH > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk@CapeCodHealth.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Royl1 <@t> LabCorp.com Wed Apr 29 11:33:01 2015 From: Royl1 <@t> LabCorp.com (Roy, Lisa) Date: Wed Apr 29 11:33:34 2015 Subject: [Histonet] Over- decalcified tissue In-Reply-To: References: <12ECD7346266D74691EC2BFC75285E455B26355C@BFL323E10.pathmdlabs.local> <20150429180741.30321tpr6tafai9w@correoweb.usc.es> Message-ID: <470bd8186bea402386fc6d2e3cafca9e@rtwems04.lca.labcorp.com> Good article on IHC world about restoring nuclear detail to over decalcified tissue....it is called Problem Number 23. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, April 29, 2015 12:21 PM To: pablo.sanchez@usc.es Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Over- decalcified tissue I would suggest not allowing decalcification to be extended. Better to leave in fixative until you can control the time of endpoint of decalcification. What type of decal solution are you using? I am not a big fan of trying to adjust the chemistry of the stain to compensate for over decalcification. Is there opportunity to submit another sample? Sent from my iPhone > On Apr 29, 2015, at 9:08 AM, pablo.sanchez@usc.es wrote: > > As I usually process gross pieces of bone -that need abouth thirty hours decalcifying- I suffer the same nuissance. Also would thank any hint. > > Pablo Sanchez > > > > Laurie Colbert escribiu: > >> I have tissue that was left in decal over the weekend and now has very poor nuclear staining. Is there a "fix" for this so that I can get better nuclear staining (other than restaining for a long time in the hematoxylin)? >> >> Laurie Colbert, HT (ASCP) >> Histology Supervisor >> PATH MD >> 8158 Beverly Blvd. >> Los Angeles, CA 90048 >> (323) 648-3214 direct >> (424) 245-7284 main lab >> >> The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From rsrichmond <@t> gmail.com Wed Apr 29 13:04:50 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Apr 29 13:04:53 2015 Subject: [Histonet] Re: H. Pylori Testing Message-ID: Nancy Stedman observes: >>I believe IHC is more sensitive than the special stains too. One caveat for anyone who works with veterinary samples - the H. pylori antibodies are specific for H. pylori, so I have not found these antibodies to be helpful for evaluating other species with helicobacter-associated gastritis. One exception is the antibody made by Biocare which seems to stain some of the feline helicobacters, and maybe others too (have not tried).<< As far as I know, the only Helicobacter species other than H. pylori reported as a human pathogen is H. heilmanii - I've seen it exactly once, with a dye method - supposedly more common in Japan, often with a history of close association with cats. Supposedly H. heilmanii marks with the commercial IHC antibodies also. I don't think the data exist to show that IHC is more sensitive than the older dye methods, in terms of detecting clinical disease. As I noted before, the IHC is much faster for the pathologist to read. Also, many pathologists report any bacteria seen with dye methods as H. pylori, including the bacteria brought down by the endoscope from the oral cavity. Bob Richmond Samurai Pathologist Maryville TN From mburns <@t> atlanticurologyclinics.com Wed Apr 29 13:14:59 2015 From: mburns <@t> atlanticurologyclinics.com (Melissa Burns) Date: Wed Apr 29 13:16:01 2015 Subject: [Histonet] Leavitt Bx Chip Feedback Message-ID: <8F26C28A6B397C47BFC334F7B2D6FD985CC72DDF@AUC-Exchange1.gsuro.com> Has anyone had experience with the Leavitt Medical group and their BxChip system???? Looking for ANY feedback so I can prove a point :) From garreyf <@t> gmail.com Wed Apr 29 13:18:49 2015 From: garreyf <@t> gmail.com (Garreyf) Date: Wed Apr 29 13:18:55 2015 Subject: [Histonet] Re: H. Pylori Testing In-Reply-To: References: Message-ID: In the past when using giemsa stain, I came across two human cases of very long helicobacter organisms.. I was stumped the first time since I had never seen one previously. I reflexed both to immuno and both were positive with the h pylori antibody. I assume they were both heilmani. I think it was called gastrospirillum in the past.I agree the immuno stain is much faster (easier)to look at. I've never studied it but it's sensitivity is probably just a little better than giemsa. It's those cases with very few organisms that are more apt to be missed using a non-immuno type of stain. Agree about the contaminants as well .... we alway went on the seagull shaped morphology though. Garrey Sent from my iPhone > On Apr 29, 2015, at 2:04 PM, Bob Richmond wrote: > > Nancy Stedman observes: > >>> I believe IHC is more sensitive than the special stains too. One caveat > for anyone who works with veterinary samples - the H. pylori antibodies are > specific for H. pylori, so I have not found these antibodies to be helpful > for evaluating other species with helicobacter-associated gastritis. One > exception is the antibody made by Biocare which seems to stain some of the > feline helicobacters, and maybe others too (have not tried).<< > > As far as I know, the only Helicobacter species other than H. pylori > reported as a human pathogen is H. heilmanii - I've seen it exactly once, > with a dye method - supposedly more common in Japan, often with a history > of close association with cats. Supposedly H. heilmanii marks with the > commercial IHC antibodies also. > > I don't think the data exist to show that IHC is more sensitive than the > older dye methods, in terms of detecting clinical disease. As I noted > before, the IHC is much faster for the pathologist to read. Also, many > pathologists report any bacteria seen with dye methods as H. pylori, > including the bacteria brought down by the endoscope from the oral cavity. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Apr 29 13:32:23 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Apr 29 13:32:27 2015 Subject: [Histonet] Re: H. Pylori Testing In-Reply-To: References: Message-ID: Helicobacter heilmannii (sorry I misspelled it before) was named Gastrospirillum hominis when it was first described. Tight cylindrical spirals, unlike the "gull-wing" (like you learned to draw birds in the sky when you were in the fourth grade) morphology of H. pylori. I'm not sure you could see those tight cylindrical spirals clearly using IHC, and tell them from gull-wings. Sensitivity on Giemsa staining depends on microscopic technique. Real men don't use oil immersion in pathology, but you need an oil lens to make the morphologic confirmation, and to search the occasional slide that has neutrophils in the pyloric lamina propria (chronic active gastritis), and this is time-consuming and messy, and few pathologists do it. Bob Richmond Samurai Pathologist Maryville TN On Wed, Apr 29, 2015 at 2:18 PM, Garreyf wrote: > In the past when using giemsa stain, > I came across two human cases of very long helicobacter organisms.. I was > stumped the first time since I had never seen one previously. I reflexed > both to immuno and both were positive with the h pylori antibody. I assume > they were both heilmani. I think it was called gastrospirillum in the > past.I agree the immuno stain is much faster (easier)to look at. I've > never studied it but it's sensitivity is probably just a little better than > giemsa. It's those cases with very few organisms that are more apt to be > missed using a non-immuno type of stain. Agree about the contaminants as > well .... we alway went on the seagull shaped morphology though. > > > Garrey > > Sent from my iPhone > > > On Apr 29, 2015, at 2:04 PM, Bob Richmond wrote: > > > > Nancy Stedman observes: > > > >>> I believe IHC is more sensitive than the special stains too. One caveat > > for anyone who works with veterinary samples - the H. pylori antibodies > are > > specific for H. pylori, so I have not found these antibodies to be > helpful > > for evaluating other species with helicobacter-associated gastritis. One > > exception is the antibody made by Biocare which seems to stain some of > the > > feline helicobacters, and maybe others too (have not tried).<< > > > > As far as I know, the only Helicobacter species other than H. pylori > > reported as a human pathogen is H. heilmanii - I've seen it exactly once, > > with a dye method - supposedly more common in Japan, often with a history > > of close association with cats. Supposedly H. heilmanii marks with the > > commercial IHC antibodies also. > > > > I don't think the data exist to show that IHC is more sensitive than the > > older dye methods, in terms of detecting clinical disease. As I noted > > before, the IHC is much faster for the pathologist to read. Also, many > > pathologists report any bacteria seen with dye methods as H. pylori, > > including the bacteria brought down by the endoscope from the oral > cavity. > > > > Bob Richmond > > Samurai Pathologist > > Maryville TN > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wsimons <@t> athensgastro.com Wed Apr 29 13:33:53 2015 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Wed Apr 29 13:33:58 2015 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUmU6IEguIFB5bG9yaSBUZXN0aW5n?= In-Reply-To: References: Message-ID: <20150429183354.21959.qmail@server276.com> I prefer (and my pathologist) the Warthin Starry. I performed that routinely, and affordably, on the Dako Artisan at my last employer. At my current small lab we perform a Giemsa for h.pylori. I've seen two cases of H. heilmanii in the last 2 years. Both stained with Giemsa. When my lab expands I have the Artisan on my wishlist for both IHC and Warthin Starry. Thank you everyone for the comments. Wanda K. Simons, HT (ASCP) Athens Gastroenterology Association 3320 Old Jefferson Road, Bldg.400 Athens, GA 30607 www.histosearch.com/gsh/ > -------Original Message------- > From: Garreyf > To: Bob Richmond > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re: H. Pylori Testing > Sent: Apr 29 '15 14:21 > > In the past when using giemsa stain, > I came across two human cases of very long helicobacter organisms.. I was stumped the first time since I had never seen one previously. I reflexed both to immuno and both were positive with the h pylori antibody. I assume they were both heilmani. I think it was called gastrospirillum in the past.I agree the immuno stain is much faster (easier)to look at.??I've never studied it but it's sensitivity is probably just a little better than giemsa. It's those cases with very few organisms that are more apt to??be missed using a non-immuno type of stain.??Agree about the contaminants as well .... we alway went on the seagull shaped morphology though. > > > Garrey > > Sent from my iPhone > > > On Apr 29, 2015, at 2:04 PM, Bob Richmond wrote: > > > > Nancy Stedman observes: > > > >>> I believe IHC is more sensitive than the special stains too. One caveat > > for anyone who works with veterinary samples - the H. pylori antibodies are > > specific for H. pylori, so I have not found these antibodies to be helpful > > for evaluating other species with helicobacter-associated gastritis. One > > exception is the antibody made by Biocare which seems to stain some of the > > feline helicobacters, and maybe others too (have not tried).<< > > > > As far as I know, the only Helicobacter species other than H. pylori > > reported as a human pathogen is H. heilmanii - I've seen it exactly once, > > with a dye method - supposedly more common in Japan, often with a history > > of close association with cats. Supposedly H. heilmanii marks with the > > commercial IHC antibodies also. > > > > I don't think the data exist to show that IHC is more sensitive than the > > older dye methods, in terms of detecting clinical disease. As I noted > > before, the IHC is much faster for the pathologist to read. Also, many > > pathologists report any bacteria seen with dye methods as H. pylori, > > including the bacteria brought down by the endoscope from the oral cavity. > > > > Bob Richmond > > Samurai Pathologist > > Maryville TN > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Wed Apr 29 14:03:51 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Apr 29 14:03:57 2015 Subject: [Histonet] NSH - 2015 Awards Banquet Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D8BDE@SBS2K8.premierlab.local> Hello Everyone It's that time of the year again. As you know this year's NSH Convention is being held in Washington DC. The Stars & Stripes NSH Awards Ceremony & Celebration will be on Saturday August 28th. The NSH offers many awards, too numerous to list here, so to see the list of awards available head to the NSH website http://nsh.org/scholarships-awards. There are three categories for awards; leadership, education and advocacy. These awards offer financial incentives for continuing education along with recognizing the individuals within our profession who have demonstrated both dedication and excellence in the field of Histotechnology. I can't stress to you enough how great it is that the NSH can provide this much support to their members for continuing education opportunities. These opportunities are provided through the generosity of the sponsors, if it were not for them we would not have these awards. It's also so important that we take time out of our busy days to nominate individuals in our field that are raising the bar and setting a higher standard, these individuals need and should be recognized and what a better way to do that then to nominate them for an award. I challenge all of you to take the time to nominate one individual you feel is deserving of one of the many awards the NSH has to offer. If you have questions or need help in nominating a colleague or yourself please feel free to contact me at liz@premierlab.com. Thanks, have a great day and nominate!!!!. Liz NSH Awards Chairperson Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From jvickroy <@t> SpringfieldClinic.com Wed Apr 29 14:10:39 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Wed Apr 29 14:10:48 2015 Subject: [Histonet] Recycling of alcohol Message-ID: <9B1A1501A800064397369BD8072E6BCAB5FCD9@E2K10DB.springfieldclinic.com> In the past I have plenty of years of experience recycling formalin and clearing agents, however I don't have much experience with recycling reagent grade alcohols. If we recycle waste alcohol that is 95% - 100 %, I am told that our recycled product will be 99% or above. With that said how are others using this alcohol? Are they using it as 100% on the tissue processors? Are they using it in the strainers for 100%? Are others using it as 95% instead of 100%. Obvioulsy I am concerned that my last alcohol station on the VIP is as close to 100% as possible and the same is true for the automated stainer. Obviously we can use the recycled alcohol for cleaning reagents on the tissue processors. Please share with me if you are recycling alcohol to what extent are you using the recycled product. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Timothy.Morken <@t> ucsf.edu Wed Apr 29 14:17:53 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Apr 29 14:18:23 2015 Subject: [Histonet] RE: Recycling of alcohol In-Reply-To: <9B1A1501A800064397369BD8072E6BCAB5FCD9@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCAB5FCD9@E2K10DB.springfieldclinic.com> Message-ID: <761E2B5697F795489C8710BCC72141FF3681A7ED@ex07.net.ucsf.edu> Jim, yes, in my experience you are going to use this for 70 to 95% alcohol steps, not 100, unless you get a water absorber (B&R has one, at least used to). Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Wednesday, April 29, 2015 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling of alcohol In the past I have plenty of years of experience recycling formalin and clearing agents, however I don't have much experience with recycling reagent grade alcohols. If we recycle waste alcohol that is 95% - 100 %, I am told that our recycled product will be 99% or above. With that said how are others using this alcohol? Are they using it as 100% on the tissue processors? Are they using it in the strainers for 100%? Are others using it as 95% instead of 100%. Obvioulsy I am concerned that my last alcohol station on the VIP is as close to 100% as possible and the same is true for the automated stainer. Obviously we can use the recycled alcohol for cleaning reagents on the tissue processors. Please share with me if you are recycling alcohol to what extent are you using the recycled product. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Royl1 <@t> LabCorp.com Wed Apr 29 15:40:37 2015 From: Royl1 <@t> LabCorp.com (Roy, Lisa) Date: Wed Apr 29 15:40:48 2015 Subject: [Histonet] RE: Recycling of alcohol In-Reply-To: <761E2B5697F795489C8710BCC72141FF3681A7ED@ex07.net.ucsf.edu> References: <9B1A1501A800064397369BD8072E6BCAB5FCD9@E2K10DB.springfieldclinic.com> <761E2B5697F795489C8710BCC72141FF3681A7ED@ex07.net.ucsf.edu> Message-ID: <49f1e5879f584d44a246382e76324e8f@rtwems04.lca.labcorp.com> In my experiences with recycling of alcohol, you will never get any return over 95%. Our lab stopped recycling the alcohol because eventually you have a ton of 95% coming out your ears, but still purchasing reagent grade 100%. Wasn't worth it for us. Just did xylene and formalin. Lisa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, April 29, 2015 3:18 PM To: Vickroy, James; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling of alcohol Jim, yes, in my experience you are going to use this for 70 to 95% alcohol steps, not 100, unless you get a water absorber (B&R has one, at least used to). Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Wednesday, April 29, 2015 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling of alcohol In the past I have plenty of years of experience recycling formalin and clearing agents, however I don't have much experience with recycling reagent grade alcohols. If we recycle waste alcohol that is 95% - 100 %, I am told that our recycled product will be 99% or above. With that said how are others using this alcohol? Are they using it as 100% on the tissue processors? Are they using it in the strainers for 100%? Are others using it as 95% instead of 100%. Obvioulsy I am concerned that my last alcohol station on the VIP is as close to 100% as possible and the same is true for the automated stainer. Obviously we can use the recycled alcohol for cleaning reagents on the tissue processors. Please share with me if you are recycling alcohol to what extent are you using the recycled product. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From bcooper <@t> chla.usc.edu Wed Apr 29 16:07:28 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Wed Apr 29 16:07:35 2015 Subject: [Histonet] FW: ATRT delay in formalin fixation In-Reply-To: <0F1618F485EE374BACB5CC443BD67AFC5BBCBB48@CHLAEXMBH03.LA.AD.CHLA.ORG> References: <0F1618F485EE374BACB5CC443BD67AFC5BBCBB48@CHLAEXMBH03.LA.AD.CHLA.ORG> Message-ID: Good afternoon Histonet, We got a complaint from one of our researchers this morning about loss of antigenicity on an FFPE sample. According to the researcher "the pERK antigen in tissue is labile if not fixed right away, hence trying to find if the samples which are negative are truly negative or could be a factor of time to fixing. She's hypothesized that it's likely due to a delay in formalin fixation from the time of removal from the patient. When we perform frozen sections, the tissue that's removed sits in a petri dish fresh until the decision is made on how to proceed with the sample. By the time frozen samples are prepared and tissue is set aside for ancillary studies, it can be as long as 30 minutes until the remaining tissue is placed in formalin. Have any of you had complaints of antigenicity loss due to this delay? We've only had one researcher make this complaint, and it's only on cases of AT/RT. None of our pathologists have noticed this phenomenon. Is this exclusive for this "pERK antigen"? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From amosbrooks <@t> gmail.com Wed Apr 29 16:48:04 2015 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Apr 29 16:48:07 2015 Subject: [Histonet] H. Pylori Testing Message-ID: Hi, If you are looking at the possibility of doing IHC manually, I would really recommend the Shandon Sequenza. It is a box that holds clips that attach to your slides. The slides are held vertically and you drop reagents into the top. The reagents replace the previous reagent which drips into a catch tray below. It makes it **really** easy to reproducibly do IHC manually. Amos Brooks On Tue, Apr 28, 2015 at 12:24 PM, wrote: > Message: 8 > Date: Mon, 27 Apr 2015 16:49:25 -0400 > From: Garreyf > Subject: Re: [Histonet] H. Pylori Testing > To: Michael Ann Jones > Cc: "histonet@lists.utsouthwestern.edu" > , "Vickroy, James" > > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Is it a pain in the neck to do it by hand? I'd like to bring my h pyloris > in house as well. I'm trying to create more revenue to support a 2nd > histotech. > > Garrey > > Sent from my iPhone > > > On Apr 27, 2015, at 4:11 PM, Michael Ann Jones > wrote: > > > > We recently switched to IHC stain for HP, however, before that we used > > Giemsa regressively for those. Not as sensitive as IHC. For IHC we used > > Cell Marque by hand (with great results) and now we are automated. > > > > Michael Ann > > Michael Ann Jones, HT (ASCP) > > Histology Manager > > Metropath > > 7444 W. Alaska Dr. #250 > > Lakewood, CO 80226 > > 303.634.2511 > > Mjones@metropath.com > From contact <@t> excaliburpathology.com Wed Apr 29 17:12:06 2015 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed Apr 29 17:15:09 2015 Subject: [Histonet] H. Pylori Testing In-Reply-To: References: Message-ID: <322692228.993543.1430345526566.JavaMail.yahoo@mail.yahoo.com> Love, Love, Love the Sequenza. It is all I use.??Paula Pierce,BS, HTL(ASCP)HT?President?Excalibur Pathology, Inc.?5830 N Blue Lake Dr.?Norman, OK 73069?405-759-3953 PH 405-759-7513 FAX?www.excaliburpathology.com From: Amos Brooks To: "histonet@lists.utsouthwestern.edu" Sent: Wednesday, April 29, 2015 4:48 PM Subject: [Histonet] H. Pylori Testing Hi, ? ? If you are looking at the possibility of doing IHC manually, I would really recommend the Shandon Sequenza. It is a box that holds clips that attach to your slides. The slides are held vertically and you drop reagents into the top. The reagents replace the previous reagent which drips into a catch tray below. It makes it **really** easy to reproducibly do IHC manually. Amos Brooks On Tue, Apr 28, 2015 at 12:24 PM, wrote: > Message: 8 > Date: Mon, 27 Apr 2015 16:49:25 -0400 > From: Garreyf > Subject: Re: [Histonet] H. Pylori Testing > To: Michael Ann Jones > Cc: "histonet@lists.utsouthwestern.edu" >? ? ? ? ,? ? "Vickroy, James" >? ? ? ? > Message-ID: > Content-Type: text/plain;? ? ? charset=us-ascii > > Is it a pain in the neck to do it? by hand? I'd like to bring my h pyloris > in house as well. I'm trying to create more revenue to support a 2nd > histotech. > > Garrey > > Sent from my iPhone > > > On Apr 27, 2015, at 4:11 PM, Michael Ann Jones > wrote: > > > > We recently switched to IHC stain for HP, however, before that we used > > Giemsa regressively for those. Not as sensitive as IHC. For IHC we used > > Cell Marque by hand (with great results) and now we are automated. > > > > Michael Ann > > Michael Ann Jones, HT (ASCP) > > Histology Manager > > Metropath > > 7444 W. Alaska Dr. #250 > > Lakewood, CO 80226 > > 303.634.2511 > > Mjones@metropath.com > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From latecor <@t> adinet.com.uy Wed Apr 29 22:24:45 2015 From: latecor <@t> adinet.com.uy (C.D.G.) Date: Wed Apr 29 22:24:35 2015 Subject: [Histonet] Over- decalcified tissue In-Reply-To: <20150429180741.30321tpr6tafai9w@correoweb.usc.es> References: <12ECD7346266D74691EC2BFC75285E455B26355C@BFL323E10.pathmdlabs.local> <20150429180741.30321tpr6tafai9w@correoweb.usc.es> Message-ID: <5541A07D.5010402@adinet.com.uy> Laurie and Pablo, doing a soft antigen retrieval in a citrate buffer solution pH6 for 10 minutes after hydrating your slides will allow you to improve the quality of nuclear stain. El 29/04/2015 a las 13:07, pablo.sanchez@usc.es escribi?: > As I usually process gross pieces of bone -that need abouth thirty > hours decalcifying- I suffer the same nuissance. Also would thank any > hint. > > Pablo Sanchez > > > > Laurie Colbert escribiu: > >> I have tissue that was left in decal over the weekend and now has >> very poor nuclear staining. Is there a "fix" for this so that I can >> get better nuclear staining (other than restaining for a long time in >> the hematoxylin)? >> >> Laurie Colbert, HT (ASCP) >> Histology Supervisor >> PATH MD >> 8158 Beverly Blvd. >> Los Angeles, CA 90048 >> (323) 648-3214 direct >> (424) 245-7284 main lab >> >> The information contained in this transmission may contain privileged >> and confidential information, including patient information protected >> by federal and state privacy laws. It is intended only for the use of >> the person(s) named above. If you are not the intended recipient, you >> are hereby notified that any review, dissemination, distribution, or >> duplication of this communication is strictly prohibited. If you are >> not the intended recipient, please contact the sender by reply email >> and destroy all copies of the original message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From c7138 <@t> hotmail.com Thu Apr 30 10:40:50 2015 From: c7138 <@t> hotmail.com (Carolyn Nelson) Date: Thu Apr 30 10:52:51 2015 Subject: [Histonet] (no subject) Message-ID: Hi, I was hoping someone can help me with tissue falling off the slides. I have tried regular slides with and without adhesive in the water bath. Charged slides with and without adhesive in the water bath. I have not changed the type of slides I?m using. All the chemicals are fresh in the processor and the stain line, as well as the paraffin in the processor. It is the worst on needle bx ( prostate and breast ). I am SO frustrated, any help would be greatly appreciated! Carolyn Sent from Windows Mail From Royl1 <@t> LabCorp.com Thu Apr 30 11:05:28 2015 From: Royl1 <@t> LabCorp.com (Roy, Lisa) Date: Thu Apr 30 11:09:04 2015 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <495d694d59fc4bd2886ffb57f5ff1360@rtwems04.lca.labcorp.com> How long do you bake slides for before staining, at what temperature? Does your stainer use agitation? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carolyn Nelson Sent: Thursday, April 30, 2015 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I was hoping someone can help me with tissue falling off the slides. I have tried regular slides with and without adhesive in the water bath. Charged slides with and without adhesive in the water bath. I have not changed the type of slides I?m using. All the chemicals are fresh in the processor and the stain line, as well as the paraffin in the processor. It is the worst on needle bx ( prostate and breast ). I am SO frustrated, any help would be greatly appreciated! Carolyn Sent from Windows Mail -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From sadrew <@t> wisc.edu Thu Apr 30 12:35:34 2015 From: sadrew <@t> wisc.edu (Sally Ann Drew) Date: Thu Apr 30 12:35:38 2015 Subject: [Histonet] TMA Arrayers Message-ID: <014001d0836c$10069d80$3013d880$@wisc.edu> I was wondering how many people have semi-automated or automated tissue microarray equipment? I am especially interested in those who graduated from manual methods to the automated and how they reviewed and ranked the limited options out there. Thank you! _Sally Sally Ann Drew, MT(ASCP) UWSMPH-Dept. of Pathology TRIP Lab Manager Translational Science BioCore CSC, L5/181 608.265.1093 From hfedor <@t> jhmi.edu Thu Apr 30 12:46:56 2015 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Apr 30 12:47:03 2015 Subject: [Histonet] TMA Arrayers In-Reply-To: <014001d0836c$10069d80$3013d880$@wisc.edu> References: <014001d0836c$10069d80$3013d880$@wisc.edu> Message-ID: Hello, We have the Pathology Devices Semi automated units. We are happy with them. Our decision to keep with the less automated units are largely due to our work flow. We make TMA's for customers. But the customer has to do all of the work in the design and data entry into out TMAJ Database. I think all of the automated units have data input features that are not compatible with this work flow. Good luck in choosing. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St? Room 310 Basement| Bond St Annex Building Baltimore, MD?| 21231 410-614-1660 http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Ann Drew Sent: Thursday, April 30, 2015 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TMA Arrayers I was wondering how many people have semi-automated or automated tissue microarray equipment? I am especially interested in those who graduated from manual methods to the automated and how they reviewed and ranked the limited options out there. Thank you! _Sally Sally Ann Drew, MT(ASCP) UWSMPH-Dept. of Pathology TRIP Lab Manager Translational Science BioCore CSC, L5/181 608.265.1093 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From s.shah <@t> garvan.org.au Thu Apr 30 14:16:02 2015 From: s.shah <@t> garvan.org.au (Shruti Shah) Date: Thu Apr 30 14:17:50 2015 Subject: [Histonet] RE: TRAP stain Message-ID: Hi Everyone, can some one give me advice .... am I able to use metal staining rack for TRAP stain. Does Pararosaniline Dye or nepthol not going to react with metal or create contaminant. Thank you in advance. Shruti Shah | Research Assistant Bone Biology Group Bone Biology Division Garvan Institute of Medical Research 383 Victoria Street, Darlinghurst, NSW 2010 T: +61 (0) 2 9295 8278 | | E: s.shah@garvan.org.au ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Helen Fedor [hfedor@jhmi.edu] Sent: Friday, 1 May 2015 03:46 To: Sally Ann Drew; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] TMA Arrayers Hello, We have the Pathology Devices Semi automated units. We are happy with them. Our decision to keep with the less automated units are largely due to our work flow. We make TMA's for customers. But the customer has to do all of the work in the design and data entry into out TMAJ Database. I think all of the automated units have data input features that are not compatible with this work flow. Good luck in choosing. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St Room 310 Basement| Bond St Annex Building Baltimore, MD | 21231 410-614-1660 http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Ann Drew Sent: Thursday, April 30, 2015 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TMA Arrayers I was wondering how many people have semi-automated or automated tissue microarray equipment? I am especially interested in those who graduated from manual methods to the automated and how they reviewed and ranked the limited options out there. Thank you! _Sally Sally Ann Drew, MT(ASCP) UWSMPH-Dept. of Pathology TRIP Lab Manager Translational Science BioCore CSC, L5/181 608.265.1093 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE Please consider the environment before printing this email. This message and any attachments are intended for the addressee named and may contain legally privileged/confidential/copyright information. If you are not the intended recipient, you should not read, use, disclose, copy or distribute this communication. If you have received this message in error please notify us at once by return email and then delete both messages. We accept no liability for the distribution of viruses or similar in electronic communications. This notice should not be removed. From DKnutson <@t> primecare.org Thu Apr 30 14:20:54 2015 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Thu Apr 30 14:23:24 2015 Subject: [Histonet] ANP.23410 Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A49C2E5C3D@EXCHANGE2K7.staprimecare.org> Hi fellow Histonetters - I was wondering if I could get some feedback from my peers on how you are dealing with the CAP standard ANP.23410 on Cryostat Decontamination. It states that this is done at defined intervals appropriate for the institution. The place I just inspected was doing a weekly decontaminating, and a more thorough one quarterly. I would like to know how often are other sites taking apart the entire cryostat chamber for decontamination. Thank you so much for sharing your process. Deanne Knutson Supervisor Anatomic Pathology ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From tony.henwood <@t> health.nsw.gov.au Thu Apr 30 14:31:34 2015 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Apr 30 14:31:50 2015 Subject: FW: [Histonet] IHC and oven temperature In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157F53EBED0@xmdb04.nch.kids> References: ,, , <6D6BD1DE8A5571489398B392A38A7157F53EAC93@xmdb04.nch.kids>, , <6D6BD1DE8A5571489398B392A38A7157F53EBED0@xmdb04.nch.kids> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EBEE3@xmdb04.nch.kids> Yes, I read the Dako IPX educational guides (5th ed) and on page 32: "No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable" Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth. Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information. Regards, Tony ________________________________________ From: Joelle Weaver [joelleweaver@hotmail.com] Sent: Saturday, 25 April 2015 5:51 AM To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood@health.nsw.gov.au > To: wdesalvo.cac@outlook.com; PREISZNE@mail.etsu.edu > Date: Fri, 24 Apr 2015 09:43:59 +0000 > Subject: RE: [Histonet] IHC and oven temperature > CC: histonet@lists.utsouthwestern.edu > > Hi temp drying shown to be a bad idea: > > Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. > > Abstract > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac@outlook.com] > Sent: Tuesday, 21 April 2015 1:56 AM > To: Preiszner, Johanna > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC and oven temperature > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes > > Sent from my iPhone > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > > > Hi Netters, > > > > is there something wrong with this logic: > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > Thanks! > > > > Hanna Preiszner > > ETSU/QCOM > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From b-frederick <@t> northwestern.edu Thu Apr 30 14:35:22 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Apr 30 14:35:39 2015 Subject: [Histonet] Question Message-ID: <8464cf90c79445098748ffb0e6c04f36@evcspmbx03.ads.northwestern.edu> All, I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of water." I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From rjbuesa <@t> yahoo.com Thu Apr 30 14:56:02 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Apr 30 14:56:08 2015 Subject: [Histonet] IHC and oven temperature In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157F53EBEE3@xmdb04.nch.kids> References: <6D6BD1DE8A5571489398B392A38A7157F53EBEE3@xmdb04.nch.kids> Message-ID: <1835135147.1524546.1430423762046.JavaMail.yahoo@mail.yahoo.com> What about HIER at 95-98?C? "Everybody" uses it to "enhance" epitopes detection.Is there not an intrinsic contradiction here? Ren? J.? On Thursday, April 30, 2015 3:32 PM, Tony Henwood (SCHN) wrote: Yes, I read the Dako IPX educational guides (5th ed) and on page 32: "No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable" Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth. Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information. Regards, Tony ________________________________________ From: Joelle Weaver [joelleweaver@hotmail.com] Sent: Saturday, 25 April 2015 5:51 AM To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood@health.nsw.gov.au > To: wdesalvo.cac@outlook.com; PREISZNE@mail.etsu.edu > Date: Fri, 24 Apr 2015 09:43:59 +0000 > Subject: RE: [Histonet] IHC and oven temperature > CC: histonet@lists.utsouthwestern.edu > > Hi temp drying shown to be a bad idea: > > Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. > > Abstract > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac@outlook.com] > Sent: Tuesday, 21 April 2015 1:56 AM > To: Preiszner, Johanna > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC and oven temperature > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes > > Sent from my iPhone > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > > > Hi Netters, > > > > is there something wrong with this logic: > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > Thanks! > > > > Hanna Preiszner > > ETSU/QCOM > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Thursday, April 30, 2015 3:32 PM, Tony Henwood (SCHN) wrote: Yes, I read the Dako IPX educational guides (5th ed) and on page 32: "No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable" Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth. Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information. Regards, Tony ________________________________________ From: Joelle Weaver [joelleweaver@hotmail.com] Sent: Saturday, 25 April 2015 5:51 AM To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood@health.nsw.gov.au > To: wdesalvo.cac@outlook.com; PREISZNE@mail.etsu.edu > Date: Fri, 24 Apr 2015 09:43:59 +0000 > Subject: RE: [Histonet] IHC and oven temperature > CC: histonet@lists.utsouthwestern.edu > > Hi temp drying shown to be a bad idea: > > Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. > > Abstract > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac@outlook.com] > Sent: Tuesday, 21 April 2015 1:56 AM > To: Preiszner, Johanna > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC and oven temperature > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes > > Sent from my iPhone > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > > > Hi Netters, > > > > is there something wrong with this logic: > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > Thanks! > > > > Hanna Preiszner > > ETSU/QCOM > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Thu Apr 30 15:13:38 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Apr 30 15:26:20 2015 Subject: [Histonet] RE: Question In-Reply-To: <8464cf90c79445098748ffb0e6c04f36@evcspmbx03.ads.northwestern.edu> References: <8464cf90c79445098748ffb0e6c04f36@evcspmbx03.ads.northwestern.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF3681BBFB@ex07.net.ucsf.edu> Bernice, You have to buy the 10N solution. You can only dilute a given normal solution, you cannot concentrate them. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, April 30, 2015 12:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Question All, I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of water." I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> rwjms.rutgers.edu Thu Apr 30 15:36:32 2015 From: mcauliff <@t> rwjms.rutgers.edu (Geoff) Date: Thu Apr 30 15:36:28 2015 Subject: [Histonet] Question In-Reply-To: <8464cf90c79445098748ffb0e6c04f36@evcspmbx03.ads.northwestern.edu> References: <8464cf90c79445098748ffb0e6c04f36@evcspmbx03.ads.northwestern.edu> Message-ID: <55429250.8010509@umdnj.edu> Make your own with NaOH. Dissolve 40 g NaOH in about 90 ml of distilled water. Use caution, the reaction is exothermic. When dissolved dilute to 100 ml with distilled. Keep tightly closed to avoid picking up CO2 from the air. Geoff On 4/30/2015 3:35 PM, Bernice Frederick wrote: > All, > I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of water." I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error? > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff@rwjms.rutgers.edu ********************************************** From joelleweaver <@t> hotmail.com Thu Apr 30 15:57:32 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Thu Apr 30 15:57:36 2015 Subject: [Histonet] ANP.23410 In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A49C2E5C3D@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A49C2E5C3D@EXCHANGE2K7.staprimecare.org> Message-ID: Usually I have done weekly , but in rather high usage places. With more strict downtimes and decontamination for TB or other suspected infectious cases. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: DKnutson@primecare.org > To: histonet@lists.utsouthwestern.edu > Date: Thu, 30 Apr 2015 14:20:54 -0500 > Subject: [Histonet] ANP.23410 > > Hi fellow Histonetters - > > I was wondering if I could get some feedback from my peers on how you are dealing with the CAP standard > ANP.23410 on Cryostat Decontamination. > It states that this is done at defined intervals appropriate for the institution. > The place I just inspected was doing a weekly decontaminating, and a more thorough one quarterly. > > I would like to know how often are other sites taking apart the entire cryostat chamber for decontamination. > > Thank you so much for sharing your process. > > Deanne Knutson > Supervisor > Anatomic Pathology > > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cjohnson <@t> nmda.nmsu.edu Thu Apr 30 15:59:35 2015 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Thu Apr 30 15:59:40 2015 Subject: [Histonet] Disopsal of containers? Message-ID: This may sound like a really sophomoric question but I'll ask anyway. How do you dispose of specimen containers from your histo tissues once they are emptied and rinsed? This question came up at work (veterinary diagnostic lab) and I am not aware of a standard or a reference to go to for an answer. Thanks in advance. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From Richard.Cartun <@t> hhchealth.org Thu Apr 30 16:33:05 2015 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Apr 30 16:33:13 2015 Subject: [Histonet] IHC billing question Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From wdesalvo.cac <@t> outlook.com Thu Apr 30 16:42:03 2015 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu Apr 30 16:42:18 2015 Subject: [Histonet] IHC billing question In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> Message-ID: We have to manually review the IHC billing also and continue to audit. It took billing and IT three months to create the logic to automate billing for a specimen and account for combination of there being the possibility of 88341, 88342 & 88344 and the proper combinations. Sent from my iPhone > On Apr 30, 2015, at 2:34 PM, Cartun, Richard wrote: > > Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Apr 30 16:43:25 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Apr 30 16:44:45 2015 Subject: [Histonet] RE: IHC billing question In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> Message-ID: Ouch! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Apr 30 16:44:16 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Apr 30 16:44:51 2015 Subject: [Histonet] IHC billing question In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> Message-ID: We do all of our IHC billing manually. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, April 30, 2015 4:43 PM To: Cartun, Richard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question We have to manually review the IHC billing also and continue to audit. It took billing and IT three months to create the logic to automate billing for a specimen and account for combination of there being the possibility of 88341, 88342 & 88344 and the proper combinations. Sent from my iPhone > On Apr 30, 2015, at 2:34 PM, Cartun, Richard wrote: > > Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf <@t> gmail.com Thu Apr 30 16:52:25 2015 From: garreyf <@t> gmail.com (Garreyf) Date: Thu Apr 30 16:52:30 2015 Subject: [Histonet] IHC billing question In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> Message-ID: Your Lis should not have done that. If you are using Copath/Cerner, I think they have automated it now according to their most recent newsletter. Currently, I have to manually change the 88342's to 1's It's somewhat of a pain. But, your Lis should have consulted someone before deleting all codes. Your pathologists should have been on top of it as well imho. Unfortunately, someone has to change. It may be too late to bill too for some. Garrey Sent from my iPhone > On Apr 30, 2015, at 5:44 PM, Mike Pence wrote: > > We do all of our IHC billing manually. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Thursday, April 30, 2015 4:43 PM > To: Cartun, Richard > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC billing question > > We have to manually review the IHC billing also and continue to audit. It took billing and IT three months to create the logic to automate billing for a specimen and account for combination of there being the possibility of 88341, 88342 & 88344 and the proper combinations. > > Sent from my iPhone > >> On Apr 30, 2015, at 2:34 PM, Cartun, Richard wrote: >> >> Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & Immunopathology >> Director, Biospecimen Collection Programs Assistant Director, Anatomic >> Pathology Hartford Hospital >> 80 Seymour Street >> Hartford, CT 06102 >> (860) 972-1596 >> (860) 545-2204 Fax >> >> >> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Thu Apr 30 18:03:27 2015 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Apr 30 18:03:37 2015 Subject: [Histonet] IHC billing question In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> , Message-ID: We do our billing manually through CoPath as well. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Mike Pence [mpence@grhs.net] Sent: Thursday, April 30, 2015 5:44 PM To: 'WILLIAM DESALVO'; Cartun, Richard Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC billing question We do all of our IHC billing manually. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, April 30, 2015 4:43 PM To: Cartun, Richard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question We have to manually review the IHC billing also and continue to audit. It took billing and IT three months to create the logic to automate billing for a specimen and account for combination of there being the possibility of 88341, 88342 & 88344 and the proper combinations. Sent from my iPhone > On Apr 30, 2015, at 2:34 PM, Cartun, Richard wrote: > > Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Apr 30 18:19:22 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Apr 30 18:19:48 2015 Subject: [Histonet] RE... Frozen section fixation problems Message-ID: <000001d0839c$17b85e80$47291b80$@bresnan.net> I have been following this with interest both now and in the past. A word of caution about the acetone/ethanol fixation. I did NOT use the acetone/alcohol fixative cold, but at RT (as it was taught to me by an IHC expert). That is a bonus since you don't have to maintain A/A fixative in a refrigerator. It could be that in Brett's hands, A/A at -20C works well so I can't argue with a successful variation for this fixative. A major caveat: A/A is used for rodent CD markers and cannot be used for human CD4 or CD8 as reported by the late Dr. Chris van der Loos. He and I collaborated about frozen section fixatives many times along with trying each other's method. He always had success with 4C acetone in very humid The Netherlands but was careful to air dry the sections overnight in front of a fan. These two human CD markers do not tolerate ethanol consequently, I wouldn't use A/A for any human CD marker work. We have used it exclusively for murine and rat CD markers and Q-fever organisms. A good rule it to have a panel of fixation methods in order to optimize fixation for any given antigen. I do not understand why Patrick has such problems using cold acetone fixation which leads to poor sections. We air dried frozen sections for a minimum of 30 min before A/A fixation. Most of the time, frozen sections were cut and immediately dried at RT for up to 4 hours, then stored in a box containing only one day's worth of staining. The unfixed sections are stored at -80C with a bag of silica gel in the box (25 slide capacity). The slide box can be taken out the night before staining, or even the day of staining with lid on to NOT GET WATER CONDENSATION ON THE SECTION. Water condensation can damage morphology and antigens. I would NEVER use an acetone gradient for fixation since the increase in water could be a cause of the damage. Water is not going to maintain isotonic conditions and prevent damage. If you want to blow away a frozen section after acetone fixation, just rinse with water........a sure way to damage the morphology. After acetone fixation only (10 min at 4C), air dry section for 15 min, then go into PBS or TBS. Before fixation, use barrier pen i.e. ImmEdge (vortexed to mix components before drawing around section) from Vector around section, then fix in A/A 10 min @RT and then go immediately from A/A into pure PBS for 3 changes. The 4th change is PBS/0.2% Tween 20 to equilibrate the section for IHC buffer conditions. What I suspect, after so many continued problems, is the snap freezing of the tissue may be done improperly and the damage could be excessive freezing artifact. Something is amiss and it may be BEFORE FIXATION with the acetone. In general, I have found methanol to be a poor fixative for IHC, and should be totally avoided for any CD marker work since it causes protein hydrolysis of the epitope causing weak, poor staining. 4% paraformaldehyde @ 4C without antigen retrieval can give weak staining and antigen retrieval with frozen sections has to be done carefully to maintain delicate sections on the slide. 95%, even 100%, ethanol can also result in weak staining. You did not say what epitopes you are trying to preserve and stain for? I don't think the plus charge slides are the culprit since I had labs using acetone fixation of FS on plain glass slides before Plus charge was so popular. Are you sure your PBS or TBS is correctly made? Incorrectly made PBS caused morphology havoc to completely blow away my frozen sections. This led to purchasing Sigma Dulbecco PBS which never gave problems. Maybe you can describe more of what you are doing from the time you receive and snap freeze the tissues, species, etc., including manual or automated staining in order to have other help you chase away these annoying "gremlins". Gayle M. Callis HTL/HT/MT(ASCP) Patrick, We do a lot of frozen section IHC work. Years ago Gayle Callis turned me on to fixing in cold acetone:ethanol (3:1) . We keep it at -20C and I fix for 10 min. on the bench then wash in PBS and proceed with the IHC. We do dry slides for at least 30 min before fixing. This has worked well in our hands for many different antibodies. Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly <@t> merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Tuesday, April 28, 2015 5:56 PM To: (Histonet <@t> lists.utsouthwestern.edu) Subject: [Histonet] Acetone fixation problems with OCT Tissues Hi Everyone, I am still having issues with my IHCs with Acetone fixation. If I fix in 100% Acetone, I get IHC staining, but my tissues are 50-90% destroyed. If I fix in 4% paraformaldehyde, or 10% NBF or (95% Etoh and/or Methanol with Acetone) I lose the epitopes I either get no staining or very weak staining, but the tissue morphology look fine. I just tried an acetone gradient where I cut the tissues at 5 uM and dried them overnight, then fixed for 10 minutes in 100% acetone, then fixed in 95% acetone for 1 minute, then fixed in 70% acetone for 30 seconds, then quick rinsed in H20, then washed as normal in DPBS pH 7.4. I did 4 slides, 2 slides with one company's Charged slides ,and 2 slides with another company's charged slides. One company's slides look completely destroyed, the others may turn out, it was hard to tell how much damage there was. I'll know tomorrow when I finish staining and Hemotoxylin them. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 From jkiernan <@t> uwo.ca Thu Apr 30 18:24:10 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Apr 30 18:24:14 2015 Subject: FW: [Histonet] IHC and oven temperature In-Reply-To: <7380ddcb419ec.5542b98d@uwo.ca> References: <6D6BD1DE8A5571489398B392A38A7157F53EAC93@xmdb04.nch.kids> <6D6BD1DE8A5571489398B392A38A7157F53EBED0@xmdb04.nch.kids> <6D6BD1DE8A5571489398B392A38A7157F53EBEE3@xmdb04.nch.kids> <72e099b337665.5542a27d@uwo.ca> <7210a7223599c.5542a2b9@uwo.ca> <72e08fcf34e58.5542a2f8@uwo.ca> <72f0f7cd31dc1.5542a336@uwo.ca> <71f0e3043462f.5542a375@uwo.ca> <7360adfc33a1b.5542a3b3@uwo.ca> <7300aeff36043.5542a3f1@uwo.ca> <7390d1de33b0b.5542a430@uwo.ca> <72e0ce7936014.5542a46e@uwo.ca> <72e0cb853420d.5542a6a5@uwo.ca> <72b08bb645783.5542b1f1@uwo.ca> <73e0efd541544.5542b22f@uwo.ca> <72909be645f99.5542b26e@uwo.ca> <7290e2e945983.5542b2ad@uwo.ca> <7330860e45333.5542b2eb@uwo.ca> <73309c9544dac.5542b32a@uwo.ca> <72b0cf3940226.5542b368@uwo.ca> <72a0a3e5477fa.5542b3a6@uwo.ca> <73c0a23445420.5542b3e5@uwo.ca> <72b0c16445f75.5542b45f@uwo.ca> <7340cbf544835.5542b562@uwo.ca> <73c0a79d47f73.5542b8ad@uwo.ca> <73e0bb0c422f0.5542b8eb@uwo.ca> <7380ee8540fbc.5542b92a@uwo.ca> <7380c51441965.5542b968@uwo.ca> <7380ddcb419ec.5542b98d@uwo.ca> Message-ID: <7300978f470c4.5542734a@uwo.ca> The statement quoted by Tony from the Dako manual cannot be true because many antigens have to be exposed to water at 100C in order to be immunostained - antigen retrieval. Denaturation of a macromolecule by heat increases the number of exposed epitopes, which typically are short amino acid sequences that bind specifically to the Fab segments of antibody molecules. On the other hand, it is easy to believe that 60C would denature antibody molecules enough to damage their binding sites and impair or prevent immunostaining. According to AWP Vermeer and W Norde (2000), the Fab segments of IgG were denatured when the temperature of a solution slightly exceeded 60C. ("The Thermal Stability of Immunoglobulin: Unfolding and Aggregation of a Multi-Domain Protein" Biophysical Journal 78: 394?404.) They found that further heating denatured the Fc segment, but the changed molecules became entangled and aggregated before denaturation was complete. Microwave heating is sometimes used to accelerate immunostaining, but control of the temperature is critical. For example: ME Boon & E Marani (1991) "The major importance of temperature data in publications concerning microwave techniques" European Journal of Morphology 29: 181?183. ?John Kiernan London, Canada = = = On 30/04/15, "Tony Henwood (SCHN)" wrote: > > Yes, > > I read the Dako IPX educational guides (5th ed) and on page 32: > "No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable" > Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth. > > Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information. > > Regards, > Tony > > ________________________________________ > From: Joelle Weaver [joelleweaver@hotmail.com] > Sent: Saturday, 25 April 2015 5:51 AM > To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] IHC and oven temperature > > I remember reading that the preferred temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > From: tony.henwood@health.nsw.gov.au > > To: wdesalvo.cac@outlook.com; PREISZNE@mail.etsu.edu > > Date: Fri, 24 Apr 2015 09:43:59 +0000 > > Subject: RE: [Histonet] IHC and oven temperature > > CC: histonet@lists.utsouthwestern.edu > > > > Hi temp drying shown to be a bad idea: > > > > Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. > > > > Abstract > > > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac@outlook.com] > > Sent: Tuesday, 21 April 2015 1:56 AM > > To: Preiszner, Johanna > > Cc: histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] IHC and oven temperature > > > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes > > > > Sent from my iPhone > > > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > > > > > Hi Netters, > > > > > > is there something wrong with this logic: > > > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > > > Thanks! > > > > > > Hanna Preiszner > > > ETSU/QCOM > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************************* > > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > > ********************************************************************************* > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >