[Histonet] RE: Squamous cells staining on H&E and IHC

[Tony Henwood (SCHN)]

Would you believe that according to a few very imminent histotechnologists, the source of nearly all of the squames contaminating slides is dandruff (a little embarrassing!!) 

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor
Sent: Wednesday, 29 October 2014 9:44 PM
To: Amber McKenzie; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Squamous cells staining on H&E and IHC

When the sections are cut and taken on the slide from water bath, slide must only holds on the frosted end of the slides don't touch other part of slide.
Regards
Muhammad Tahseen
MLT (JIMTEF) Japan
Histology Supervisor
SKMCH&RC
Lahore
Pakistan

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Wednesday, October 29, 2014 12:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Squamous cells staining on H&E and IHC


Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's?  I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting?  Change out water bath several times during shifts? Any suggestions?  Thanks!

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