From MAUGER <@t> email.chop.edu Wed Oct 1 06:49:01 2014 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Wed Oct 1 06:49:29 2014 Subject: [Histonet] RE: Glacial Acetic Acid subs. In-Reply-To: References: Message-ID: <929548F049BC6D448DF9488B556449BE16570F38@EXCMBXPW5.chop.edu> Vinegar should work. Jo Mauger -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Armstrong, Karoleigh T Sent: Tuesday, September 30, 2014 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Glacial Acetic Acid subs. Does any one know of a substitute for Glacial Acetic Acid? We use it to clean the lines on the VIP and in the Eosin. Our Corp. heads want to eliminate Glacial in concentration form from all its hospitails. Thanks, Karoleigh Armstrong, HT (ASCP) -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbireir <@t> yahoo.com Wed Oct 1 11:53:33 2014 From: mbireir <@t> yahoo.com (Manahil) Date: Wed Oct 1 11:53:41 2014 Subject: [Histonet] Quality Management Message-ID: <9ED68044-5120-4E18-8A6F-8EC560C218A1@yahoo.com> Hi histonet, Please if you can help me to have an idea about AP Quality management- SOP. Thanks, Manahil Elbireir Sent from my iPhone From ADuddey <@t> firsthealth.org Thu Oct 2 11:14:11 2014 From: ADuddey <@t> firsthealth.org (Duddey, Aimee) Date: Thu Oct 2 11:14:18 2014 Subject: [Histonet] RE: JCAHO Competency Requirements In-Reply-To: References: Message-ID: <09FBA01CA9B6374A83C5C76E09E4618812648EA9@EXMAIL1-FHC.firsthealth.org> We were recently JCAHO inspected and I asked the same question of the inspector. The response I got was because histology does not report results our competency would be much broader. You would still have to assess the 6 elements for each process we do. For example in our lab we do Routine H&E, Special stains, and IHC stains. You would have to assess the 6 elements for each of these. In the leading practice library for TJC there is a very good competency assessment document that lists the 6 elements. Hope this helps. Aimee -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Tuesday, September 23, 2014 10:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] JCAHO Competency Requirements Good Morning! We are switching from CAP to JCAHO for our inspection and accreditation. I'm reading a lot about "Six Method Competency", but most of the information I have is geared toward blood testing. If anyone out there can offer any suggestions as to how I should reformat our competency forms to satisfy JCAHO, I would appreciate any help. Thanks in advance! Laura Jones B.A., HT, PBT (ASCP) | Lead Tech, Histology | Community Health Systems 740 East State Street | Sharon PA | Phone (724)983-3950 | Fax (724)983-3982 www.sharonregional.com | lpjones@srhs-pa.org ________________________________ Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WaitT <@t> livemail.uthscsa.edu Thu Oct 2 13:11:08 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Thu Oct 2 13:11:15 2014 Subject: [Histonet] Decalcified Bone Eosin Overstaining Message-ID: <1412273468352.90401@livemail.uthscsa.edu> Hey histonetters! I'm currently doing some H&E Staining for EDTA Decalcified Bone Tissue and it seems that the tissue is staining very intensely with Eosin...perhaps too much. Was curious to see from some of the more experienced histotechnicians out there which methods you guys have used in the past to perhaps fix an issue like this. Here is my protocol that I have used once I have sectioned the paraffin embedded tissue. I have omitted the 1 second Acid Alcohol Dip in a few of my trials and it seemed that this method worked great to keep the tissue from overstaining with Eosin. However, this is an important step for differentiation of the Hematoxylin...so I'm a little puzzled about what to do next. Perhaps wash with tap water again after the Acid Alcohol? 1. Xylene - 3 Minutes 2. Xylene 3- Minutes 3. Xylene - 3 Minutes 4. 100% EtOH - 3 Minutes 5. 100% EtOH - 3 Minutes 6. 95% EtOH - 3 Minutes 7. 70 % EtOH - 3 Minutes 8. Filtered Harris Hematoxylin (Commercial Purchase) - 6 Minutes 9. Wash with running Tap water 10. 1 second dip in 0.5% 12M HCL Acid Alcohol 11. Eosin Y Solution - 1 Minute 12. 70% EtOH - 2 Minutes 13. 70% EtOH - 2 Minutes 14. 95% EtOH - 2 Minutes 15. 95% EtOH - 2 Minutes 16. 100% EtOH - 2 Minutes 17. 100% EtOH - 2 Minutes 18. Xylene - 1 Minute 19. Xylene - 1 Minute 20. Xylene - 1 Minute Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry From HornHV <@t> archildrens.org Thu Oct 2 13:43:07 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Oct 2 13:43:12 2014 Subject: [Histonet] RE: Decalcified Bone Eosin Overstaining In-Reply-To: <1412273468352.90401@livemail.uthscsa.edu> References: <1412273468352.90401@livemail.uthscsa.edu> Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFF4B@EVS1.archildrens.org> When the bones are over decaled there will be very little hematoxylin staining and too much eosin. You can use a buffer before the hematoxylin to aid the hematoxylin in staining. I think one/two dip(s) in eosin will be sufficient. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Thursday, October 02, 2014 1:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decalcified Bone Eosin Overstaining Hey histonetters! I'm currently doing some H&E Staining for EDTA Decalcified Bone Tissue and it seems that the tissue is staining very intensely with Eosin...perhaps too much. Was curious to see from some of the more experienced histotechnicians out there which methods you guys have used in the past to perhaps fix an issue like this. Here is my protocol that I have used once I have sectioned the paraffin embedded tissue. I have omitted the 1 second Acid Alcohol Dip in a few of my trials and it seemed that this method worked great to keep the tissue from overstaining with Eosin. However, this is an important step for differentiation of the Hematoxylin...so I'm a little puzzled about what to do next. Perhaps wash with tap water again after the Acid Alcohol? 1. Xylene - 3 Minutes 2. Xylene 3- Minutes 3. Xylene - 3 Minutes 4. 100% EtOH - 3 Minutes 5. 100% EtOH - 3 Minutes 6. 95% EtOH - 3 Minutes 7. 70 % EtOH - 3 Minutes 8. Filtered Harris Hematoxylin (Commercial Purchase) - 6 Minutes 9. Wash with running Tap water 10. 1 second dip in 0.5% 12M HCL Acid Alcohol 11. Eosin Y Solution - 1 Minute 12. 70% EtOH - 2 Minutes 13. 70% EtOH - 2 Minutes 14. 95% EtOH - 2 Minutes 15. 95% EtOH - 2 Minutes 16. 100% EtOH - 2 Minutes 17. 100% EtOH - 2 Minutes 18. Xylene - 1 Minute 19. Xylene - 1 Minute 20. Xylene - 1 Minute Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Ronald.Houston <@t> nationwidechildrens.org Thu Oct 2 14:03:47 2014 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Oct 2 14:05:10 2014 Subject: [Histonet] melanoma control Message-ID: Anyone have a spare Melanoma control block (something positive for HMB-45, Melan-A and Tyrosinase)? Can trade for something you may need. We have a ton of C3d/C4d positive rejected kidney blocks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From msjessicaphillips <@t> gmail.com Thu Oct 2 14:46:14 2014 From: msjessicaphillips <@t> gmail.com (Jessica Phillips) Date: Thu Oct 2 14:46:21 2014 Subject: [Histonet] HT Certification Message-ID: Hello all. I have been following histonet postings for the last year and I just want to let everyone know how much I appreciate all of the information. I am currently going through in house training for my histotechnician certification at Stanford Medical Center. I am attempting to make my study cards for the special stain portion of the examination and I am having a little trouble. I see on ASCP's website a list of probable stains on the exam but NSH's website also has a suggested list of stains that should also be studied. NSH's list is longer and more involved and while I understand that it is worth my while to know the theory behind all of the staining on these two lists, I would like to know if anyone has an opinion about which list is more relevant to passing the exam, if any. Any type of comment is greatly appreciated. All of the histotechs in my lab have taken the exam over 10 years ago and are not able to offer specifics. Thanks much! -Jessica From msjessicaphillips <@t> gmail.com Thu Oct 2 15:17:52 2014 From: msjessicaphillips <@t> gmail.com (Jessica Phillips) Date: Thu Oct 2 15:17:57 2014 Subject: [Histonet] Re: HT Certification In-Reply-To: References: Message-ID: Thanks for the advice! I actually have read all of the Carson text and I have her companion flash cards and work book. I also have been reviewing the ASCP HT certification book. I guess I'm just paranoid about all of the possible stains on the exam. My B.S. is in health science and my minor is in clinical science so I have a grasp of staining mechanisms but I just want to be as prepared as possible. On Oct 2, 2014 12:46 PM, "Jessica Phillips" wrote: > Hello all. I have been following histonet postings for the last year and I > just want to let everyone know how much I appreciate all of the > information. I am currently going through in house training for my > histotechnician certification at Stanford Medical Center. I am attempting > to make my study cards for the special stain portion of the examination and > I am having a little trouble. I see on ASCP's website a list of probable > stains on the exam but NSH's website also has a suggested list of stains > that should also be studied. NSH's list is longer and more involved and > while I understand that it is worth my while to know the theory behind all > of the staining on these two lists, I would like to know if anyone has an > opinion about which list is more relevant to passing the exam, if any. Any > type of comment is greatly appreciated. All of the histotechs in my lab > have taken the exam over 10 years ago and are not able to offer specifics. > > Thanks much! > > -Jessica > From gayle.callis <@t> bresnan.net Thu Oct 2 16:11:55 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Oct 2 16:12:36 2014 Subject: [Histonet] Re: Decalcified bone eosin overstaining Message-ID: <000501cfde85$807fa7a0$817ef6e0$@bresnan.net> What Hazel is saying is true but with acid decalcifiers i.e. formic, nitric or HCl. These acids damage nucleic acids aka acid hydrolysisand if the decalcification is not controlled by endpoint testing, then the nuclei will appear pale and in some cases, don't stain at all ("over-decalcification"). However, with EDTA is not going to damage the nucleic acids, and you should have normal hematoxylin and eosin staining. After EDTA decalcification, we had good H&E staining. After acid decalcification, we had over-staining with eosin and reduced staining time in eosin to 10 continouslu moving dips, followed by three 95% and three 100% and clearing. I think your problem is going from acid/alcohol directly into the eosin, a no no. This does NOT allow for correct staining of Eosin at pH 4 to 4.5. Because you now have such an acidic environment, this is probably the cause of eosin over staining - often an ugly brick red color. You should have running tap water rinsing after hematoxylin for at least 1 minute, also do a 1 minute water rinse after the acid/alcohol. You obviously answered your own question "Perhaps a tap water rinse after the Acid Alcohol?" and that is a resounding YES!!! Where is your bluing step? You are leaving your differentiated hematoxylin in a reddish/blue state. A correct sequence for Harris Hematoxylin staining: Deparaffinize: Xylene x 3 changes 100% x 2 changes 95% x 2 changes 70% x 1 change Distilled water rinse - 1 minute to hydrate the section Harris Hematoxylin - up to 10 minutes Tap water rinse - 1 minute 0.5% Acid/alcohol - 1 dip. Do this quickly and go right into running tap water. Tap water rinse - 1 minute Water should flow start under and go up over the slides. Bluing solution - 1 minute This is a very mild base. Buy a commercial solution specified for Harris hematoxylin. Tap water rinse - 1 minute 70% alcohol - 1 minute Eosin - time desired 95% alcohol X 3 changes 100% x 3 changes Clearing You will see the sections turn blue after a bluing solution which requires a tap water rinse and also a 70% alcohol rinse make sure there are no bluing solution cations carried over into the eosin. Optimal pH for eosin staining is 4 to 4.5 (which you have altered by going from acid/alcohol directly into eosin). A 70% alcohol also equilibrates to the alcohol percentage of the eosin solution. If the eosin still is over staining, reduce time to 30 sec. If dipping, be sure you are move the slides up and down continuously. After the eosin you should be able to just rinse in 95% alcohols to differentiate out excess eosin. If any of the post-eosin alcohol rinses have too much eosin carryover, you will continue to stain the bone. Make sure alcohol rinses after eosin are fresh or at least rotated so there is a fresh last 95% and fresh last 100%. Any pink color in the last 100% means you have water carryover which can cause 1) cloudiness 2) eosin bleeding after cover slipping. On the side and many years ago, we used Harris Hematoxylin for acid decalcified bone followed by running tap water rinse but eliminated the acid/alcohol step entirely to avoid even weaker nuclear staining already caused by acid hydrolysis from the acid decalcifier. A bluing solution was used followed by a water rinse before eosin staining sequence. I learned this from the AFIP bone lab and it worked with excellent results. However, you are working with EDTA decalcified bone and when you eliminated the acid/alcohol, it worked great without eosin over staining. Good Luck Gayle M. Callis HTL/HT/MT(ASCP) You wrote: When the bones are over decaled there will be very little hematoxylin staining and too much eosin. You can use a buffer before the hematoxylin to aid the hematoxylin in staining. I think one/two dip(s) in eosin will be sufficient. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv <@t> archildrens.org archildrens.org Sent: Thursday, October 02, 2014 1:11 PM To: histonet <@t> lists.utsouthwestern.edu Subject: [Histonet] Decalcified Bone Eosin Overstaining Hey histonetters! I'm currently doing some H&E Staining for EDTA Decalcified Bone Tissue and it seems that the tissue is staining very intensely with Eosin...perhaps too much. Was curious to see from some of the more experienced histotechnicians out there which methods you guys have used in the past to perhaps fix an issue like this. Here is my protocol that I have used once I have sectioned the paraffin embedded tissue. I have omitted the 1 second Acid Alcohol Dip in a few of my trials and it seemed that this method worked great to keep the tissue from overstaining with Eosin. However, this is an important step for differentiation of the Hematoxylin...so I'm a little puzzled about what to do next. Perhaps wash with tap water again after the Acid Alcohol? 1. Xylene - 3 Minutes 2. Xylene 3- Minutes 3. Xylene - 3 Minutes 4. 100% EtOH - 3 Minutes 5. 100% EtOH - 3 Minutes 6. 95% EtOH - 3 Minutes 7. 70 % EtOH - 3 Minutes 8. Filtered Harris Hematoxylin (Commercial Purchase) - 6 Minutes 9. Wash with running Tap water 10. 1 second dip in 0.5% 12M HCL Acid Alcohol 11. Eosin Y Solution - 1 Minute 12. 70% EtOH - 2 Minutes 13. 70% EtOH - 2 Minutes 14. 95% EtOH - 2 Minutes 15. 95% EtOH - 2 Minutes 16. 100% EtOH - 2 Minutes 17. 100% EtOH - 2 Minutes 18. Xylene - 1 Minute 19. Xylene - 1 Minute 20. Xylene - 1 Minute Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Oct 2 19:04:47 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Oct 2 19:05:09 2014 Subject: [Histonet] RE: Decalcified Bone Eosin Overstaining In-Reply-To: <1412273468352.90401@livemail.uthscsa.edu> References: <1412273468352.90401@livemail.uthscsa.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E021C7B7@xmdb04.nch.kids> The increased eosinophilia, in this scenario, is usually due to over-decalcification. I would suggest: * Treat sections with 1% Periodic acid (as used in the PAS stain) for 10 minutes before Haematoxylin staining. * Use an iron-haematoxylin or Celestine blue-Haematoxylin * decrease time in eosin (or use a neutral 1% eosin stain). This should increase nuclear staining and decrease the eosin reaction. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Friday, 3 October 2014 4:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Decalcified Bone Eosin Overstaining Hey histonetters! I'm currently doing some H&E Staining for EDTA Decalcified Bone Tissue and it seems that the tissue is staining very intensely with Eosin...perhaps too much. Was curious to see from some of the more experienced histotechnicians out there which methods you guys have used in the past to perhaps fix an issue like this. Here is my protocol that I have used once I have sectioned the paraffin embedded tissue. I have omitted the 1 second Acid Alcohol Dip in a few of my trials and it seemed that this method worked great to keep the tissue from overstaining with Eosin. However, this is an important step for differentiation of the Hematoxylin...so I'm a little puzzled about what to do next. Perhaps wash with tap water again after the Acid Alcohol? 1. Xylene - 3 Minutes 2. Xylene 3- Minutes 3. Xylene - 3 Minutes 4. 100% EtOH - 3 Minutes 5. 100% EtOH - 3 Minutes 6. 95% EtOH - 3 Minutes 7. 70 % EtOH - 3 Minutes 8. Filtered Harris Hematoxylin (Commercial Purchase) - 6 Minutes 9. Wash with running Tap water 10. 1 second dip in 0.5% 12M HCL Acid Alcohol 11. Eosin Y Solution - 1 Minute 12. 70% EtOH - 2 Minutes 13. 70% EtOH - 2 Minutes 14. 95% EtOH - 2 Minutes 15. 95% EtOH - 2 Minutes 16. 100% EtOH - 2 Minutes 17. 100% EtOH - 2 Minutes 18. Xylene - 1 Minute 19. Xylene - 1 Minute 20. Xylene - 1 Minute Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From WaitT <@t> livemail.uthscsa.edu Fri Oct 3 06:50:43 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Fri Oct 3 06:50:50 2014 Subject: [Histonet] Re: Decalcified Bone Eosin Overstaining In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157E021C7B7@xmdb04.nch.kids> References: <1412273468352.90401@livemail.uthscsa.edu>, <6D6BD1DE8A5571489398B392A38A7157E021C7B7@xmdb04.nch.kids> Message-ID: Wow these are awesome, thanks everybody for feedback! So appreciated Sent from my iPhone > On Oct 2, 2014, at 7:05 PM, "Tony Henwood (SCHN)" wrote: > > The increased eosinophilia, in this scenario, is usually due to over-decalcification. > > I would suggest: > > * Treat sections with 1% Periodic acid (as used in the PAS stain) for 10 minutes before Haematoxylin staining. > * Use an iron-haematoxylin or Celestine blue-Haematoxylin > * decrease time in eosin (or use a neutral 1% eosin stain). > > This should increase nuclear staining and decrease the eosin reaction. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan > Sent: Friday, 3 October 2014 4:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Decalcified Bone Eosin Overstaining > > Hey histonetters! I'm currently doing some H&E Staining for EDTA Decalcified Bone Tissue and it seems that the tissue is staining very intensely with Eosin...perhaps too much. Was curious to see from some of the more experienced histotechnicians out there which methods you guys have used in the past to perhaps fix an issue like this. Here is my protocol that I have used once I have sectioned the paraffin embedded tissue. I have omitted the 1 second Acid Alcohol Dip in a few of my trials and it seemed that this method worked great to keep the tissue from overstaining with Eosin. However, this is an important step for differentiation of the Hematoxylin...so I'm a little puzzled about what to do next. Perhaps wash with tap water again after the Acid Alcohol? > > > 1. Xylene - 3 Minutes > > 2. Xylene 3- Minutes > > 3. Xylene - 3 Minutes > > 4. 100% EtOH - 3 Minutes > > 5. 100% EtOH - 3 Minutes > > 6. 95% EtOH - 3 Minutes > > 7. 70 % EtOH - 3 Minutes > > 8. Filtered Harris Hematoxylin (Commercial Purchase) - 6 Minutes > > 9. Wash with running Tap water > > 10. 1 second dip in 0.5% 12M HCL Acid Alcohol > > 11. Eosin Y Solution - 1 Minute > > 12. 70% EtOH - 2 Minutes > > 13. 70% EtOH - 2 Minutes > > 14. 95% EtOH - 2 Minutes > > 15. 95% EtOH - 2 Minutes > > 16. 100% EtOH - 2 Minutes > > 17. 100% EtOH - 2 Minutes > > 18. Xylene - 1 Minute > > 19. Xylene - 1 Minute > > 20. Xylene - 1 Minute > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* From bszpunar <@t> umail.iu.edu Fri Oct 3 11:06:24 2014 From: bszpunar <@t> umail.iu.edu (Bryan Szpunar) Date: Fri Oct 3 11:06:27 2014 Subject: [Histonet] Re: HT Certification Message-ID: Hi Jessica, When I sat for the exam ~4 years ago, there were quite a lot of special stain related questions. My main study guide was Carson, so if you are very familiar with her text you should be pretty well off. Being able to identify stains, tissue types, and troubleshoot a given stain I would say are all valuable. Also, remember to heed the "adaptive" nature of the test (you can find more on that by searching the archives). From speaking to others, there is generally a consensus that an inverse relationship exists between how hard one felt the test was and a passing score. Those who felt like the questions were extremely difficult, tend to pass because they are answering higher level questions. Good luck! I think you'll be fine... -Bryan From jerrysedgewick <@t> gmail.com Fri Oct 3 11:25:52 2014 From: jerrysedgewick <@t> gmail.com (Jerry Sedgewick) Date: Fri Oct 3 11:25:56 2014 Subject: [Histonet] Free Webinar: Avoid Errors in Quantification Message-ID: For those of you who quantify optical densities of DAB, IP, etc.; and for those who measure for areas, counts, etc (morphometry); please read below regarding a free webinar. Note that the webinar will present a new product and software solution. I encourage you to listen, even if you are not in a position to purchase this product/software solution, because you will learn about linearity, the importance of linear tones when performing optical density measurements, and the possibility that your camera system is not producing linear images and is, therefore, providing an image that contains erroneous data. Morphometry and Density/Intensity Readings: Avoid Errors in Quantification ------------------------------ Tuesday, October 21, 2014 1:00 PM - 1:30 PM EDT Webinar Registration: https://www2.gotomeeting.com/register/945357514 Introduction by Michael Linden, MD, PhD (Assistant Professor, Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN) Presenter: Jerry Sedgewick (Imaging and Analysis, LLC; author, consultant and recognized authority in post-processing, analysis and quantification of scientific images) If you have done quantitation, you know that inconsistent images require subjective means for setting thresholds when segmenting objects for measurement. You need an objective method to set thresholds for measurement of area, length, count, etc., and be able to apply that method across multiple images. When quantifying densities and intensities, it is imperative that images contain equal increments of grey or color values across the dynamic range (image linearity). Can you trust your images? A recent survey found that approximately 50% of color camera systems offer software settings that can result in non-linear color images. If you?ve been quantifying immunostained cells or cellular structures (i.e. DAB, BCIP/NBT, X-Gal, etc.), you may have presented false data. This webinar explores quantitative methods that employ a single threshold for any number of color brightfield images. You will learn how to create consistent images, without subjective adjustments, for subsequent quantization. You will learn how to confirm your imaging system delivers scientific images that are linear. You will also learn how to correct images taken over a time course while keeping tones and colors linear. -- Jerry (Gerald) Sedgewick quickphotoshop.com http://www.imagingandanalysis.com Author: "Scientific Imaging with Photoshop: Methods, Measurement and Output" Automated Image Measurement services and systems; On-Site and on-line training for image acquisition/processing/quantitation in science, microscopy and medicine. From Allison.Scott <@t> harrishealth.org Fri Oct 3 12:22:38 2014 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Fri Oct 3 12:22:43 2014 Subject: [Histonet] Reccomended temperature for storing blocks and slides Message-ID: Happy Friday to everyone. Does anyone know what the recommended temperature is for storing blocks and slides? Your help in this would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From abadesuyi <@t> nrh-ok.com Fri Oct 3 13:07:10 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Fri Oct 3 13:07:17 2014 Subject: [Histonet] New CPT Codes for IHC Message-ID: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> Hi, I hope you guys are doing well. Please I have a question about billing the IHC. My question is do we bill by the number of antibodies or blocks? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From Joyce.Weems <@t> emoryhealthcare.org Fri Oct 3 13:33:26 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Oct 3 13:33:35 2014 Subject: [Histonet] RE: New CPT Codes for IHC In-Reply-To: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> Message-ID: For non-Medicare it is per block - CPT code 88342. For Medicare - per specimen. G0461 for the first antibody and G0462 for each additional different ab. Best, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Friday, October 03, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CPT Codes for IHC Hi, I hope you guys are doing well. Please I have a question about billing the IHC. My question is do we bill by the number of antibodies or blocks? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From azdudley <@t> hotmail.com Fri Oct 3 14:47:50 2014 From: azdudley <@t> hotmail.com (anita) Date: Fri Oct 3 14:47:54 2014 Subject: [Histonet] slide id Message-ID: just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. thanks for your input, anita dudley providence hosp mobile alabama From chesarato <@t> hotmail.com Fri Oct 3 14:50:10 2014 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Fri Oct 3 14:50:12 2014 Subject: [Histonet] Re: Decalcified Bone Eosin Overstaining Message-ID: In the past I have been consulted for lost of DAB staining in IHC when using acid alcohol to differentiate hematoxylin. In my Latitude tap water is enough for bluing hematoxylin but I think that you need something else.Trizma Base is a good bluing agent when tap water is not good enough.Just put a spoon of trizma in water and see the results. Cesar RomeroBuenos AiresArgentina From mpence <@t> grhs.net Fri Oct 3 14:50:37 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Oct 3 14:51:32 2014 Subject: [Histonet] slide id In-Reply-To: References: Message-ID: We do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita Sent: Friday, October 03, 2014 2:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide id just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. thanks for your input, anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sheila.Tapper <@t> EssentiaHealth.org Fri Oct 3 14:55:10 2014 From: Sheila.Tapper <@t> EssentiaHealth.org (Tapper, Sheila J.) Date: Fri Oct 3 14:55:21 2014 Subject: [Histonet] slide id In-Reply-To: References: Message-ID: We have our techs fast process the slides that they cut - so the tech is documented in the processing history... same for embedding. Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, October 03, 2014 2:51 PM To: 'anita'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] slide id We do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita Sent: Friday, October 03, 2014 2:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide id just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. thanks for your input, anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Fri Oct 3 14:59:42 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Fri Oct 3 14:59:47 2014 Subject: [Histonet] slide id In-Reply-To: References: Message-ID: <12ECD7346266D74691EC2BFC75285E4538114F03@BFL323E10.pathmdlabs.local> We do. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita Sent: Friday, October 03, 2014 12:48 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide id just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. thanks for your input, anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Fri Oct 3 15:01:32 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Fri Oct 3 15:01:45 2014 Subject: [Histonet] slide id In-Reply-To: References: Message-ID: We use Vantage Quality and Tracking system and the tech is captured for embedding, microtomy and staining Sent from my iPhone > On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. wrote: > > We have our techs fast process the slides that they cut - so the tech is documented in the processing history... same for embedding. > > Sheila > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Friday, October 03, 2014 2:51 PM > To: 'anita'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] slide id > > We do. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita > Sent: Friday, October 03, 2014 2:50 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] slide id > > just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. > > thanks for your input, > > anita dudley > > providence hosp > > mobile alabama > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne <@t> rwjuh.edu Fri Oct 3 15:17:20 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Fri Oct 3 15:17:26 2014 Subject: [Histonet] slide id In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> How do you like Vantage? Have you experienced any problems with it, and is it easy to use? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Friday, October 03, 2014 4:02 PM To: Tapper, Sheila J. Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] slide id We use Vantage Quality and Tracking system and the tech is captured for embedding, microtomy and staining Sent from my iPhone > On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. wrote: > > We have our techs fast process the slides that they cut - so the tech is documented in the processing history... same for embedding. > > Sheila > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Friday, October 03, 2014 2:51 PM > To: 'anita'; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] slide id > > We do. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita > Sent: Friday, October 03, 2014 2:50 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] slide id > > just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. > > thanks for your input, > > anita dudley > > providence hosp > > mobile alabama > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Fri Oct 3 15:17:53 2014 From: suetp918 <@t> comcast.net (Sue) Date: Fri Oct 3 15:18:01 2014 Subject: [Histonet] slide id In-Reply-To: References: Message-ID: <1830634960.391939.1412367473966.JavaMail.root@comcast.net> We use Ventana tracking system that is interfaced with our LIS and can track all slides. ? STP TJUH From suetp918 <@t> comcast.net Fri Oct 3 15:19:43 2014 From: suetp918 <@t> comcast.net (Sue) Date: Fri Oct 3 15:19:51 2014 Subject: [Histonet] slide id In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> Message-ID: <1720459039.392129.1412367583638.JavaMail.root@comcast.net> like it very much, was not sure if I was going to like going back to labels but it is not as bad as I though.? Only issue is the cost but I am working with other vendors to get cheaper labels. ? STP TJUH From wdesalvo.cac <@t> outlook.com Fri Oct 3 15:20:39 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Fri Oct 3 15:20:52 2014 Subject: [Histonet] slide id In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> Message-ID: Vantage works great. We use it to track all blocks and slides as they move in and out of the core lab and in and out of archive. You must bar code and make a decision on cassette printers. Techs scan their own bar code to log on the system. Sent from my iPhone > On Oct 3, 2014, at 1:17 PM, Rathborne, Toni wrote: > > How do you like Vantage? Have you experienced any problems with it, and is it easy to use? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Friday, October 03, 2014 4:02 PM > To: Tapper, Sheila J. > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] slide id > > We use Vantage Quality and Tracking system and the tech is captured for embedding, microtomy and staining > > Sent from my iPhone > >> On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. wrote: >> >> We have our techs fast process the slides that they cut - so the tech is documented in the processing history... same for embedding. >> >> Sheila >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence >> Sent: Friday, October 03, 2014 2:51 PM >> To: 'anita'; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] slide id >> >> We do. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita >> Sent: Friday, October 03, 2014 2:50 PM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] slide id >> >> just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. >> >> thanks for your input, >> >> anita dudley >> >> providence hosp >> >> mobile alabama >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bcooper <@t> chla.usc.edu Fri Oct 3 15:26:20 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Fri Oct 3 15:26:28 2014 Subject: [Histonet] slide id In-Reply-To: References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> Message-ID: How does Vantage work with blocks and slides that were in your institution prior to the implementation of Vantage? Suppose you needed to send materials to another institution for further testing? Do you generate new barcoded labels and affix them to the materials prior to sending them out (for tracking purposes)? Thanks, Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Friday, October 03, 2014 1:21 PM To: Rathborne, Toni Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J. Subject: Re: [Histonet] slide id Vantage works great. We use it to track all blocks and slides as they move in and out of the core lab and in and out of archive. You must bar code and make a decision on cassette printers. Techs scan their own bar code to log on the system. Sent from my iPhone > On Oct 3, 2014, at 1:17 PM, Rathborne, Toni wrote: > > How do you like Vantage? Have you experienced any problems with it, and is it easy to use? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Friday, October 03, 2014 4:02 PM > To: Tapper, Sheila J. > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] slide id > > We use Vantage Quality and Tracking system and the tech is captured for embedding, microtomy and staining > > Sent from my iPhone > >> On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. wrote: >> >> We have our techs fast process the slides that they cut - so the tech is documented in the processing history... same for embedding. >> >> Sheila >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence >> Sent: Friday, October 03, 2014 2:51 PM >> To: 'anita'; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] slide id >> >> We do. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita >> Sent: Friday, October 03, 2014 2:50 PM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] slide id >> >> just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. >> >> thanks for your input, >> >> anita dudley >> >> providence hosp >> >> mobile alabama >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From wdesalvo.cac <@t> outlook.com Fri Oct 3 15:31:55 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Fri Oct 3 15:32:09 2014 Subject: [Histonet] slide id In-Reply-To: References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> Message-ID: We decided to not reprint cassettes or labels on blocks/slides produced prior to Vantage. Vantage is a move forward (2 years and counting) process and we continue to follow manual process for pre-Vantage material. Each user will have to make a decision on how to handle existing material. Sent from my iPhone > On Oct 3, 2014, at 1:26 PM, Cooper, Brian wrote: > > How does Vantage work with blocks and slides that were in your institution prior to the implementation of Vantage? Suppose you needed to send materials to another institution for further testing? Do you generate new barcoded labels and affix them to the materials prior to sending them out (for tracking purposes)? > > Thanks, > > Brian > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Friday, October 03, 2014 1:21 PM > To: Rathborne, Toni > Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J. > Subject: Re: [Histonet] slide id > > Vantage works great. We use it to track all blocks and slides as they move in and out of the core lab and in and out of archive. You must bar code and make a decision on cassette printers. Techs scan their own bar code to log on the system. > > Sent from my iPhone > >> On Oct 3, 2014, at 1:17 PM, Rathborne, Toni wrote: >> >> How do you like Vantage? Have you experienced any problems with it, and is it easy to use? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO >> Sent: Friday, October 03, 2014 4:02 PM >> To: Tapper, Sheila J. >> Cc: Histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] slide id >> >> We use Vantage Quality and Tracking system and the tech is captured for embedding, microtomy and staining >> >> Sent from my iPhone >> >>> On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. wrote: >>> >>> We have our techs fast process the slides that they cut - so the tech is documented in the processing history... same for embedding. >>> >>> Sheila >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence >>> Sent: Friday, October 03, 2014 2:51 PM >>> To: 'anita'; Histonet@lists.utsouthwestern.edu >>> Subject: RE: [Histonet] slide id >>> >>> We do. >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita >>> Sent: Friday, October 03, 2014 2:50 PM >>> To: Histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] slide id >>> >>> just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. >>> >>> thanks for your input, >>> >>> anita dudley >>> >>> providence hosp >>> >>> mobile alabama >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > From joelleweaver <@t> hotmail.com Fri Oct 3 15:43:01 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Oct 3 15:43:04 2014 Subject: [Histonet] Re: HT Certification In-Reply-To: References: Message-ID: There is information about the CAT , (computer adapted testing format) and the scaling of question difficulty on the ASCP site that is helpful to know for your test taking strategy and preparation. The general gist is that they present on the topics and questions in a category randomly, and if you answer correctly the questions increase in difficulty until you answer incorrectly. It starts over with this process through the topics in random presentation order. Sometimes they put in "test questions" they are trying out for future exams that do not count towards your scaled score. I think it is helpful to understand how this works going in. If you do not take a lot of multiple choice tests, I think it is also worth the time to do some research on how to approach the mostly multiple choice question format and how to use a good strategy for arriving at the "best" answer from the provided choices. This helps when you hit a question you are unsure about, and do not want to use too much of your time on working through one question. The other thing I would suggest, is that you have to keep in mind not to filter through your own particular laboratory, but general practices and the theory as outlined in the recommended references on the reading list. Sometimes people have said they have been frustrated when they answered a question based on their own direct experiences with their lab's procedures and they did not consider this correct. Just keep in mind, they are using a broad perspective when crafting the questions , not how it is applied in a particular lab. They also ask about the whole spectrum and not just standard clinical histotechnology, so review the EM, neuro stains and some of the specialty type things too on the study list that they consider part of histology practice, especially if you do not perform and so have limited direct experience to the technique in your lab. Other than the ideas above, try to be as rested and as relaxed as you can on test day, and you should feel prepared to perform your best on the exam. Good luck! Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 3 Oct 2014 12:06:24 -0400 > From: bszpunar@umail.iu.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: HT Certification > > Hi Jessica, > > When I sat for the exam ~4 years ago, there were quite a lot of special > stain related questions. My main study guide was Carson, so if you are very > familiar with her text you should be pretty well off. Being able to > identify stains, tissue types, and troubleshoot a given stain I would say > are all valuable. > > Also, remember to heed the "adaptive" nature of the test (you can find more > on that by searching the archives). From speaking to others, there is > generally a consensus that an inverse relationship exists between how hard > one felt the test was and a passing score. Those who felt like the > questions were extremely difficult, tend to pass because they are answering > higher level questions. > > Good luck! I think you'll be fine... > > -Bryan > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 3 17:02:28 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Oct 3 17:02:42 2014 Subject: FW: [Histonet] slide id In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679C986@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF3679C986@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF3679C994@ex07.net.ucsf.edu> Anita, in the past with hand-written labels they had to put their initials on each slide. They also had a log page for the blocks they cut and they would sign that off. Now we have Copath AB&T barcoding and each person logs in and their name is attached to anything they scan. Now it takes only seconds to find out who did anything with any materials. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita Sent: Friday, October 03, 2014 12:48 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide id just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. thanks for your input, anita dudley providence hosp mobile alabama _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 3 17:36:45 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Oct 3 17:37:18 2014 Subject: [Histonet] slide id In-Reply-To: References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF3679C9D4@ex07.net.ucsf.edu> Brian, we use Copath AB&T, not Vantage, but the principle is the same. We use the old non-barcoded system for older block/slide sendouts. There is no way to barcode old blocks, unless you want to enter it as a consult, and that entails giving it a new number, which would just confuse things. It will take a few years but eventually we'll be sending out mostly barcoded items. This is just one of the things you have to work with when changing over to something new. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Friday, October 03, 2014 1:26 PM To: WILLIAM DESALVO; Rathborne, Toni Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J. Subject: RE: [Histonet] slide id How does Vantage work with blocks and slides that were in your institution prior to the implementation of Vantage? Suppose you needed to send materials to another institution for further testing? Do you generate new barcoded labels and affix them to the materials prior to sending them out (for tracking purposes)? Thanks, Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Friday, October 03, 2014 1:21 PM To: Rathborne, Toni Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J. Subject: Re: [Histonet] slide id Vantage works great. We use it to track all blocks and slides as they move in and out of the core lab and in and out of archive. You must bar code and make a decision on cassette printers. Techs scan their own bar code to log on the system. Sent from my iPhone > On Oct 3, 2014, at 1:17 PM, Rathborne, Toni wrote: > > How do you like Vantage? Have you experienced any problems with it, and is it easy to use? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > WILLIAM DESALVO > Sent: Friday, October 03, 2014 4:02 PM > To: Tapper, Sheila J. > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] slide id > > We use Vantage Quality and Tracking system and the tech is captured > for embedding, microtomy and staining > > Sent from my iPhone > >> On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. wrote: >> >> We have our techs fast process the slides that they cut - so the tech is documented in the processing history... same for embedding. >> >> Sheila >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike >> Pence >> Sent: Friday, October 03, 2014 2:51 PM >> To: 'anita'; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] slide id >> >> We do. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita >> Sent: Friday, October 03, 2014 2:50 PM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] slide id >> >> just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. >> >> thanks for your input, >> >> anita dudley >> >> providence hosp >> >> mobile alabama >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Fri Oct 3 18:07:54 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Fri Oct 3 18:08:02 2014 Subject: [Histonet] slide id In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679C9D4@ex07.net.ucsf.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> <761E2B5697F795489C8710BCC72141FF3679C9D4@ex07.net.ucsf.edu> Message-ID: Thanks Tim. I think we're going the AB&T route as well with our next CoPath upgrade. We went to Ventana last year for their 360 tour, and saw all that Vantage has to offer. One of my good friends is a Vantage Implementation Manager, so I'm sure our decision to go with AB&T will drive him batty! Happy Friday!!! Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, October 03, 2014 3:37 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] slide id Brian, we use Copath AB&T, not Vantage, but the principle is the same. We use the old non-barcoded system for older block/slide sendouts. There is no way to barcode old blocks, unless you want to enter it as a consult, and that entails giving it a new number, which would just confuse things. It will take a few years but eventually we'll be sending out mostly barcoded items. This is just one of the things you have to work with when changing over to something new. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Friday, October 03, 2014 1:26 PM To: WILLIAM DESALVO; Rathborne, Toni Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J. Subject: RE: [Histonet] slide id How does Vantage work with blocks and slides that were in your institution prior to the implementation of Vantage? Suppose you needed to send materials to another institution for further testing? Do you generate new barcoded labels and affix them to the materials prior to sending them out (for tracking purposes)? Thanks, Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Friday, October 03, 2014 1:21 PM To: Rathborne, Toni Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J. Subject: Re: [Histonet] slide id Vantage works great. We use it to track all blocks and slides as they move in and out of the core lab and in and out of archive. You must bar code and make a decision on cassette printers. Techs scan their own bar code to log on the system. Sent from my iPhone > On Oct 3, 2014, at 1:17 PM, Rathborne, Toni wrote: > > How do you like Vantage? Have you experienced any problems with it, and is it easy to use? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > WILLIAM DESALVO > Sent: Friday, October 03, 2014 4:02 PM > To: Tapper, Sheila J. > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] slide id > > We use Vantage Quality and Tracking system and the tech is captured > for embedding, microtomy and staining > > Sent from my iPhone > >> On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. wrote: >> >> We have our techs fast process the slides that they cut - so the tech is documented in the processing history... same for embedding. >> >> Sheila >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike >> Pence >> Sent: Friday, October 03, 2014 2:51 PM >> To: 'anita'; Histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] slide id >> >> We do. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita >> Sent: Friday, October 03, 2014 2:50 PM >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] slide id >> >> just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. >> >> thanks for your input, >> >> anita dudley >> >> providence hosp >> >> mobile alabama >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From blayjorge <@t> gmail.com Sat Oct 4 10:53:12 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Sat Oct 4 10:53:17 2014 Subject: [Histonet] Best practices label + ink for slides Message-ID: Hello: Undergraduates working with me and I are about to begin generating several thousand "permanent" glass microscope slides containing micro-invertebrates. I would like to know what have you found is the best combination of label (ideally never to be removed from the slide) and print material to go as text on the labels. Please, feel free to email directly to me, blayjorge@gmail.com . Thank you. Apologies for potential multiple emails. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From tkngflght <@t> yahoo.com Sat Oct 4 13:45:13 2014 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sat Oct 4 13:45:20 2014 Subject: [Histonet] Histotech openings - Message-ID: <1412448313.2962.YahooMailNeo@web161203.mail.bf1.yahoo.com> Hello! Looking for up to three histotechs for several areas. The ability to gross is preferred, but let's talk--there is wiggle room and they'll consider all applying with experience. Send your most recent resume (no worries if it isn't current-- we can update on the phone.) Confidential - if these don't fit and you're looking for a new job, I may be able to help find you a good fit. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone & Fax admin@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From JEllin <@t> yumaregional.org Sun Oct 5 10:14:56 2014 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Sun Oct 5 10:15:05 2014 Subject: [Histonet] slide id In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679C9D4@ex07.net.ucsf.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ECFD12@vap1014.win.rwjuh.edu> , <761E2B5697F795489C8710BCC72141FF3679C9D4@ex07.net.ucsf.edu> Message-ID: We use the Sunquest PowerPath system,, it is completely Integrated and track almost all event within the facilities,, we are starting to look and track electronic assets, which are not new ideas but most systems out there are not designed with this in mind. We can print off the assets number for old cassettes and slides so we can track in from older cases since the asset ID is created from one single database,, with multiple databases there are issue with electronic assets,, this is an important fact you must discuss with your APLIS and also any third party vendor,, this becomes even more complicated when you start talking about digital slides and electronic transactions . Good luck and keep going strong Jesus Ellin Yuma Regional Medical Center Sent from my iPad > On Oct 3, 2014, at 3:37 PM, Morken, Timothy wrote: > > Brian, we use Copath AB&T, not Vantage, but the principle is the same. We use the old non-barcoded system for older block/slide sendouts. There is no way to barcode old blocks, unless you want to enter it as a consult, and that entails giving it a new number, which would just confuse things. > > It will take a few years but eventually we'll be sending out mostly barcoded items. This is just one of the things you have to work with when changing over to something new. > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian > Sent: Friday, October 03, 2014 1:26 PM > To: WILLIAM DESALVO; Rathborne, Toni > Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J. > Subject: RE: [Histonet] slide id > > How does Vantage work with blocks and slides that were in your institution prior to the implementation of Vantage? Suppose you needed to send materials to another institution for further testing? Do you generate new barcoded labels and affix them to the materials prior to sending them out (for tracking purposes)? > > Thanks, > > Brian > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Friday, October 03, 2014 1:21 PM > To: Rathborne, Toni > Cc: Histonet@lists.utsouthwestern.edu; Tapper, Sheila J. > Subject: Re: [Histonet] slide id > > Vantage works great. We use it to track all blocks and slides as they move in and out of the core lab and in and out of archive. You must bar code and make a decision on cassette printers. Techs scan their own bar code to log on the system. > > Sent from my iPhone > >> On Oct 3, 2014, at 1:17 PM, Rathborne, Toni wrote: >> >> How do you like Vantage? Have you experienced any problems with it, and is it easy to use? >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> WILLIAM DESALVO >> Sent: Friday, October 03, 2014 4:02 PM >> To: Tapper, Sheila J. >> Cc: Histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] slide id >> >> We use Vantage Quality and Tracking system and the tech is captured >> for embedding, microtomy and staining >> >> Sent from my iPhone >> >>> On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. wrote: >>> >>> We have our techs fast process the slides that they cut - so the tech is documented in the processing history... same for embedding. >>> >>> Sheila >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike >>> Pence >>> Sent: Friday, October 03, 2014 2:51 PM >>> To: 'anita'; Histonet@lists.utsouthwestern.edu >>> Subject: RE: [Histonet] slide id >>> >>> We do. >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita >>> Sent: Friday, October 03, 2014 2:50 PM >>> To: Histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] slide id >>> >>> just wondering if techs are putting their id on the slides that they cut, that way if a mistake is made with labeling the tech that cut it is identified. >>> >>> thanks for your input, >>> >>> anita dudley >>> >>> providence hosp >>> >>> mobile alabama >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From marjoh3 <@t> telus.net Sun Oct 5 11:07:45 2014 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sun Oct 5 11:07:49 2014 Subject: [Histonet] Manual For Reichert Jung Autocut 1150 Message-ID: Hi Histonetters, I am looking for a manual for a Reichert Jung Autocut microtome 1150. If anyone can provide me with this manual or a source of obtaining one would be greatly appreciated. Thank you in advance. Marilyn Johnson Apex Histology Labs. From KSimeone <@t> leavittmgt.com Mon Oct 6 06:54:11 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon Oct 6 06:54:15 2014 Subject: [Histonet] FT NIGHT POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C2FDA38A9@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann@leavittmgt.com if interested. *full time position Mon-Fri PM (will discuss hours upon interview/will be AFTER 5p) *MUST be licensed as a FL histotechnologist ONLY *MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone 561.819.6517 fax ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From wlala <@t> tulane.edu Mon Oct 6 13:49:11 2014 From: wlala <@t> tulane.edu (Trunch, Wendy L) Date: Mon Oct 6 13:49:29 2014 Subject: [Histonet] Phloxine-Tartrazine Stain Message-ID: Hi All, I am trying to stain using Phloxine-Tartrazine and then follow with immunofluorescent staining. Has anyone tried this? Any suggestions? Thanks, Wendy From ahorvath <@t> cogipath.com Mon Oct 6 14:35:32 2014 From: ahorvath <@t> cogipath.com (Andrew Horvath) Date: Mon Oct 6 14:34:36 2014 Subject: [Histonet] RE: New CPT Codes for IHC In-Reply-To: References: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> Message-ID: <004201cfe19c$b17c12c0$14743840$@cogipath.com> Actually, for non-Medicare if there are multiple stains performed on a block, where the initial CPT is 88342, any subsequent billing for stains on that block would actually be 88343 from the information I see. Please refer to the links below for more information... http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf https://www.aapc.com/memberarea/forums/showthread.php?t=101402 quote from the forum: Quite and uproar about this in our office - and a lot of confusion. Go to page 360 in this document to read Medicare's decision about this. (inactive link removed) "The CPT Editorial Panel revised the existing immunohistochemistry code, CPT code 88342 and created a new add-on code 88343 for CY 2014. Current coding requirements only allow CPT code 88342 to be billed once per specimen for each antibody, but the revised CPT codes and descriptors would allow the reporting of multiple units for each slide and each block per antibody (88342 for the first antibody and 88343 for subsequent antibodies). We believe that this coding would encourage overutilization by allowing multiple blocks and slides to be billed. To avoid this incentive, we are creating G0461 (Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain) and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (List separately in addition to code for primary procedure)) to ensure that the services are only reported once for each antibody per specimen. We believe this will result in appropriate values for these services without creating incentives for overutilization." Some of our docs are participating in conference call today with CAP to help clarify what this all means. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, October 03, 2014 12:33 PM To: 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CPT Codes for IHC For non-Medicare it is per block - CPT code 88342. For Medicare - per specimen. G0461 for the first antibody and G0462 for each additional different ab. Best, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Friday, October 03, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CPT Codes for IHC Hi, I hope you guys are doing well. Please I have a question about billing the IHC. My question is do we bill by the number of antibodies or blocks? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Mon Oct 6 15:40:47 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Oct 6 15:41:01 2014 Subject: [Histonet] RE: New CPT Codes for IHC In-Reply-To: <004201cfe19c$b17c12c0$14743840$@cogipath.com> References: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> <004201cfe19c$b17c12c0$14743840$@cogipath.com> Message-ID: Sorry, I left that part out. We don't do them, so I just didn't think. I understand the 88343 codes to be for multiple stains on the same slide. The first is 88342 additional 88343 - e.g. PIN4. So has that changed? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Andrew Horvath [mailto:ahorvath@cogipath.com] Sent: Monday, October 06, 2014 3:36 PM To: Weems, Joyce K.; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Actually, for non-Medicare if there are multiple stains performed on a block, where the initial CPT is 88342, any subsequent billing for stains on that block would actually be 88343 from the information I see. Please refer to the links below for more information... http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf https://www.aapc.com/memberarea/forums/showthread.php?t=101402 quote from the forum: Quite and uproar about this in our office - and a lot of confusion. Go to page 360 in this document to read Medicare's decision about this. (inactive link removed) "The CPT Editorial Panel revised the existing immunohistochemistry code, CPT code 88342 and created a new add-on code 88343 for CY 2014. Current coding requirements only allow CPT code 88342 to be billed once per specimen for each antibody, but the revised CPT codes and descriptors would allow the reporting of multiple units for each slide and each block per antibody (88342 for the first antibody and 88343 for subsequent antibodies). We believe that this coding would encourage overutilization by allowing multiple blocks and slides to be billed. To avoid this incentive, we are creating G0461 (Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain) and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (List separately in addition to code for primary procedure)) to ensure that the services are only reported once for each antibody per specimen. We believe this will result in appropriate values for these services without creating incentives for overutilization." Some of our docs are participating in conference call today with CAP to help clarify what this all means. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, October 03, 2014 12:33 PM To: 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CPT Codes for IHC For non-Medicare it is per block - CPT code 88342. For Medicare - per specimen. G0461 for the first antibody and G0462 for each additional different ab. Best, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Friday, October 03, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CPT Codes for IHC Hi, I hope you guys are doing well. Please I have a question about billing the IHC. My question is do we bill by the number of antibodies or blocks? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raghulj <@t> orchidpharma.com Tue Oct 7 06:35:09 2014 From: raghulj <@t> orchidpharma.com (raghulj@orchidpharma.com) Date: Tue Oct 7 06:37:46 2014 Subject: [Histonet] alizarin red s solution -query Message-ID: Dear all, We stained decalcified paraffin sections of rodent femur by alizarin red S using the following protocol - a. deparrafinised in xylene b. 100%ethaonol->95% ethaonol-->70% ethaonol-->distillled water. c. dipped in 2%alizarin red S, dissolved in distilled water (pH adjusted with 12% NH4OH to 4.26) for 2-5min d. acetone (20dips) e. aceteon: xylene (20dips) f. three changes of xylene and mounted with dpx The alizarin solution turned more colloidal during the staining process. However we went ahead staining few de paraffin slides rodent femurs. Is it in the nature of alizarin solution turning colloidal? The staining of the paraffin sections were satisfactory. Being, the first time the solution turning colloidal was surprising. Kindly suggest. J.Raghul Senior Research Associate THIS E-MAIL IS CONFIDENTIAL and intended solely for the use of the individual to whom it is addressed. If you are not the addressee, any disclosure, reproduction, copying, distribution, or other dissemination or use of this communication is strictly prohibited. Finally the recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email From barryrittman <@t> gmail.com Tue Oct 7 09:34:31 2014 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Tue Oct 7 09:34:38 2014 Subject: [Histonet] Phloxine-Tartrazine Stain In-Reply-To: References: Message-ID: Wendy if I am not mistaken, and understand that it is early in the day, phloxine absorbs at 548nm and tartrazine at around 425nm so both can show fluorescence depending on your filtering system. Barry On Mon, Oct 6, 2014 at 1:49 PM, Trunch, Wendy L wrote: > Hi All, > > I am trying to stain using Phloxine-Tartrazine and then follow with > immunofluorescent staining. Has anyone tried this? Any suggestions? > > Thanks, > Wendy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MMargiotta <@t> bmhmc.org Tue Oct 7 10:50:25 2014 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Tue Oct 7 10:50:37 2014 Subject: [Histonet] NBF% Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC69C7A064@BMH-EXCHANGE-01.BMHMC.ORG> Hi All, We recently had a recall for our 10% NBF. Our Pathologist actually noticed there was a problem with it before we received the recall notice. He's wondering if anyone tests their formalin to be sure that it is 10% and not less than that. If anyone knows of a quick way to test the percentage, please let me know. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From Kim.Kolman <@t> va.gov Tue Oct 7 11:55:27 2014 From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.) Date: Tue Oct 7 11:55:57 2014 Subject: [Histonet] need help on a cap question Message-ID: <9C32F30B6662D74A8419DDDB7E66656A091E865F@VHAV15MSGA1.v15.med.va.gov> I could use some help clarifying this question: **NEW** 04/21/2014 ANP.11525 Tissue/Cytology Assessment Record Phase I If a statement of adequacy, preliminary diagnosis, or recommendations for additional studies is provided at the time of tissue and cytology sample collection, documentation of that statement is maintained. NOTE: Documentation might include a note in the medical record or in the final report. Is this really asking that if the ordering physician wants something in particular, it must be noted somewhere? I'm hung up on the mention of 'at the time of sample collection'............. Thanks, Kim Kim Kolman Kimberly D. Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center Histology - 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000x52537 Fax: 913-758-4193 Kim.kolman@va.gov From tkngflght <@t> yahoo.com Tue Oct 7 12:13:31 2014 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Oct 7 12:13:35 2014 Subject: [Histonet] Kim's question - order documentation Message-ID: <1412702011.76602.YahooMailNeo@web161202.mail.bf1.yahoo.com> Kim- Your histonet question may not be as complicated as it might seem. Sometimes it's easier to look at these things backwards. What is the desired outcome? If there is an order -- say for a GMS--and it wasn't ordered by one of your pathologists, where did it come from? Can you track back and figure out what doctor ordered it and verify it's a valid request so the testing AND billing is appropriate (not fraudulent). When the surgeon or clinician collects the sample at the surgery or in their office, sometimes they want something specific -- say 'evaluate for fungus'. They may include this in the surgical notes, the office chart -- other places. His support staff will copy this onto the requisition or somewhere you get the request other than the requisition. If you keep copies of the req and other incoming documentation-- you've satisfied the requirement--you can track the source of the order. If you don't, include it in the gross description or notes that are transcribed onto the report so that you have a durable record that you can find (may take a while if it's the archived chart, but you can find it). This goes back to the requirement that orders can't just come from anyone or for any wild-hair reason-- and you have to be able to substantiate or prove the valid source of an ordered (and billed) test. Does that help? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab - one Great Tech at a time. 281.852.9457 Office 800.756.3309 Phone and Fax admin@fullstaff.org From mward <@t> wakehealth.edu Tue Oct 7 14:02:39 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Tue Oct 7 14:02:49 2014 Subject: [Histonet] Kim's question - order documentation In-Reply-To: <1412702011.76602.YahooMailNeo@web161202.mail.bf1.yahoo.com> References: <1412702011.76602.YahooMailNeo@web161202.mail.bf1.yahoo.com> Message-ID: I think this is an interesting question. We frequently get phone calls from clinicians asking for ER, PR, Her2 or sometimes just other IHC stains....just yesterday someone wanted CYK 7 and CYK 20 on a cytology block. We ask that they either call the pathologist who signed out the case and get them to order the stains, or with something like the breast panel, ask that they fax or email us, stating exactly what they want, the patient demographics and surgical number, etc. That way at least we have a paper trail for the files should anyone ask why we did the testing. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, October 07, 2014 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kim's question - order documentation Kim- Your histonet question may not be as complicated as it might seem. Sometimes it's easier to look at these things backwards. What is the desired outcome? If there is an order -- say for a GMS--and it wasn't ordered by one of your pathologists, where did it come from? Can you track back and figure out what doctor ordered it and verify it's a valid request so the testing AND billing is appropriate (not fraudulent). When the surgeon or clinician collects the sample at the surgery or in their office, sometimes they want something specific -- say 'evaluate for fungus'. They may include this in the surgical notes, the office chart -- other places. His support staff will copy this onto the requisition or somewhere you get the request other than the requisition. If you keep copies of the req and other incoming documentation-- you've satisfied the requirement--you can track the source of the order. If you don't, include it in the gross description or notes that are transcribed onto the report so that you have a durable record that you can find (may take a while if it's the archived chart, but you can find it). This goes back to the requirement that orders can't just come from anyone or for any wild-hair reason-- and you have to be able to substantiate or prove the valid source of an ordered (and billed) test. Does that help? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab - one Great Tech at a time. 281.852.9457 Office 800.756.3309 Phone and Fax admin@fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Kolman <@t> va.gov Tue Oct 7 14:33:11 2014 From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.) Date: Tue Oct 7 14:33:45 2014 Subject: [EXTERNAL] RE: [Histonet] Kim's question - order documentation Message-ID: <9C32F30B6662D74A8419DDDB7E66656A091E8662@VHAV15MSGA1.v15.med.va.gov> Ok I guess I'm coming from a different world; so many things, like adequacy on an FNA, FS or the like are a given, as are ER, PR, etc on tumors. We have access to patient history here so are able to get a more thorough picture of what a clinician is looking for. Of course these are all addressed in the report. Clinicians asking for something 'wild-hair' are not the last word; our pathologists have the final say on what testing may or may not be done. If the clinicians' request is not honored, I don't see the reason for noting it. I think we are well covered with our current practice. Just have to hope CAP thinks so as well.............. :) Thanks everyone! Kim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, October 07, 2014 2:03 PM To: Cheryl; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: [Histonet] Kim's question - order documentation I think this is an interesting question. We frequently get phone calls from clinicians asking for ER, PR, Her2 or sometimes just other IHC stains....just yesterday someone wanted CYK 7 and CYK 20 on a cytology block. We ask that they either call the pathologist who signed out the case and get them to order the stains, or with something like the breast panel, ask that they fax or email us, stating exactly what they want, the patient demographics and surgical number, etc. That way at least we have a paper trail for the files should anyone ask why we did the testing. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, October 07, 2014 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kim's question - order documentation Kim- Your histonet question may not be as complicated as it might seem. Sometimes it's easier to look at these things backwards. What is the desired outcome? If there is an order -- say for a GMS--and it wasn't ordered by one of your pathologists, where did it come from? Can you track back and figure out what doctor ordered it and verify it's a valid request so the testing AND billing is appropriate (not fraudulent). When the surgeon or clinician collects the sample at the surgery or in their office, sometimes they want something specific -- say 'evaluate for fungus'. They may include this in the surgical notes, the office chart -- other places. His support staff will copy this onto the requisition or somewhere you get the request other than the requisition. If you keep copies of the req and other incoming documentation-- you've satisfied the requirement--you can track the source of the order. If you don't, include it in the gross description or notes that are transcribed onto the report so that you have a durable record that you can find (may take a while if it's the archived chart, but you can find it). This goes back to the requirement that orders can't just come from anyone or for any wild-hair reason-- and you have to be able to substantiate or prove the valid source of an ordered (and billed) test. Does that help? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab - one Great Tech at a time. 281.852.9457 Office 800.756.3309 Phone and Fax admin@fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi <@t> nrh-ok.com Tue Oct 7 16:32:11 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Tue Oct 7 16:32:15 2014 Subject: [Histonet] Sakura VIP Tissue Processor Message-ID: <04EE4F75BB5FB246ADB68D69B74604439468E27921@MAIL.nrhnt.nrh-ok.com> Hi, Please i will appreciate it if you guys can tell me which one of the Sakura VIP Tissue Processors is the best. We are trying to get a new tissue processor and we don't want to make any mistake this time around. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor, Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From mpence <@t> grhs.net Wed Oct 8 09:20:01 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Oct 8 09:20:30 2014 Subject: [Histonet] Dragon and Cerner Message-ID: Hey everyone, Just wondering who out there is successfully using Dragon within the AP application of Cerner? Does it work? Not work? Are you using templates in Dragon or Cerner? You can PM me if you like. This is a peer benchmarking I would like to compare what we do now to what other Cerner users are doing. I don't want to ask this question in a Cerner forum where Cerner can control the commentary. Thanks, Mike Pence AP Supervisor From amber.mckenzie <@t> gastrodocs.net Wed Oct 8 09:24:35 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Oct 8 09:24:39 2014 Subject: [Histonet] Symphony wicking In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0112552B23@JERRY.Gia.com> For those of you who have a Symphony, how do you wick your slides? Do you cut them and put them in a basket to drain and then transfer to the trays? Do you cut them and lean them up against your water bath? Do you out them in the oven for a few minutes before you load them onto the symphony? Thanks! From suetp918 <@t> comcast.net Wed Oct 8 09:29:05 2014 From: suetp918 <@t> comcast.net (Sue) Date: Wed Oct 8 09:29:33 2014 Subject: [Histonet] Dragon and Cerner In-Reply-To: References: Message-ID: <2115505733.889404.1412778545299.JavaMail.root@comcast.net> We use Dragon with CoPath Plus and works great, you can make macros and I know my PA have created some quick texts as well.? We actually stared with Kurzweil in the 80's and expanded to Dragon speak.? the only time we do not use Dragon is with Synoptic, although it works, it is labor intensive. ? Susan T. Paturzo TJUH From Joyce.Weems <@t> emoryhealthcare.org Wed Oct 8 09:36:20 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Oct 8 09:36:42 2014 Subject: [Histonet] RE: Dragon and Cerner In-Reply-To: References: Message-ID: We use it successfully for micro. We found there was too much noise and muffling in the background for gross. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, October 08, 2014 10:20 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Dragon and Cerner Hey everyone, Just wondering who out there is successfully using Dragon within the AP application of Cerner? Does it work? Not work? Are you using templates in Dragon or Cerner? You can PM me if you like. This is a peer benchmarking I would like to compare what we do now to what other Cerner users are doing. I don't want to ask this question in a Cerner forum where Cerner can control the commentary. Thanks, Mike Pence AP Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From bcooper <@t> chla.usc.edu Wed Oct 8 09:50:50 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Wed Oct 8 09:50:55 2014 Subject: [Histonet] Symphony wicking In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC0112552B23@JERRY.Gia.com> References: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org>, <5A33C952BB67F4468AF1F36D739212BC0112552B23@JERRY.Gia.com> Message-ID: We put our slides into the Symphony trays, then bake them at 75 degrees for 15 minutes in our incubators. Then we stain with our routine H&E program, which omits baking inside the Symphony. It's faster than having a bottleneck of slides stack up in the Symphony's intellique, awaiting baking. Brian Thanks, Brian Cooper, HT (ASCP) Histology Supervisor, Path & Lab Medicine Children's Hospital, Los Angeles Sent from my Galaxy S3, so please forgive any weird typos . . . -----Original Message----- From: Amber McKenzie [amber.mckenzie@gastrodocs.net] Received: Wednesday, 08 Oct 2014, 7:27AM To: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu] Subject: [Histonet] Symphony wicking For those of you who have a Symphony, how do you wick your slides? Do you cut them and put them in a basket to drain and then transfer to the trays? Do you cut them and lean them up against your water bath? Do you out them in the oven for a few minutes before you load them onto the symphony? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From melsmith <@t> udel.edu Wed Oct 8 10:38:01 2014 From: melsmith <@t> udel.edu (Melanie Smith) Date: Wed Oct 8 10:38:25 2014 Subject: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 Message-ID: Hi everyone, My lab is trying to do caspase-3 and Ki67 double IHC stain on mouse ffpe knee joint sections, and we're having issues optimizing the caspase-3 staining. We originally tried to do fluorescent labels but had too much autofluorescence even after trying several methods of knocking it down. We decided to move on to traditional IHC and purchased a double stain IHC kit from abcam R&Rt on human/mouse tissue (DAB & AP/Red) ( http://www.abcam.com/doublestain-ihc-kit-ramprt-on-humanmouse-tissue-dab-amp-apred-ab183283.html). We started by tying to optimize both antigens independently first and had success with the Ki-67 staining (primary rat anti-ki67, eBioscience) with the abcam anti-rat secondary HRP polymer and DAB from the kit. However, we still haven?t had any luck with the caspase-3 (primary rabbit anti-caspase 3, cell signaling & abcam anti-rabbit secondary AP polymer and permanent red). We also tried the caspase-3 staining in cell culture after treating cells with an apoptosis inducer, and only saw extremely weak staining. In the FFPE sections, we have used overnight antigen retrieval in citrate buffer ph 6, at 60 C. In both the cell and FFPE sections we do an overnight primary antibody incubation at 4C. We haven?t seen any convincing staining in our FFPE sections (there is some dark pink staining but only in one or two bone marrow cells per section and it seems pretty diffuse) and also have noticed a yellow/brown precipitate forming and only showing up in the bone marrow, it also shows up in our negative antibody control sections. We also tried several different chromogen development times ranging from 10 min to an hour, and didn?t really see a difference. All sections are of course deparaffinized in xylenes and rehydrated in ethanols before starting. All washes are done with TBS-T and sections are permeabilized in 0.1% Triton X100 and blocked in 1%BSA in TBST before primary incubation. All sections are blocked with BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution from Vector after primary incubation, but before secondary incubation. If anyone has any insight into caspase-3 antibodies or doing IHC double staining that would be great! Many thanks, Melanie -- Melanie Smith, MS Research Technician II Department of Biomedical Engineering University of Delaware melsmith@udel.edu From JWatson <@t> gnf.org Wed Oct 8 10:52:17 2014 From: JWatson <@t> gnf.org (James Watson) Date: Wed Oct 8 10:52:21 2014 Subject: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 In-Reply-To: References: Message-ID: Melanie, We do this stain, we run Ventana stainers (no advertisement for Ventana intended) . We had to do this as a sequential double stain since the Caspase 3 needed polymer detection where the Ki67 only needs ABC detection. Caspase 3 was done first. Some other differences: Caspase 3 needed FC receptor block and permeablization. Ki67 needed longer HIER time than Caspase 3 so we did a second short HIER after the Caspase 3 was labeled with DAB. Jamie James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melanie Smith Sent: Wednesday, October 08, 2014 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 Hi everyone, My lab is trying to do caspase-3 and Ki67 double IHC stain on mouse ffpe knee joint sections, and we're having issues optimizing the caspase-3 staining. We originally tried to do fluorescent labels but had too much autofluorescence even after trying several methods of knocking it down. We decided to move on to traditional IHC and purchased a double stain IHC kit from abcam R&Rt on human/mouse tissue (DAB & AP/Red) ( http://www.abcam.com/doublestain-ihc-kit-ramprt-on-humanmouse-tissue-dab-amp-apred-ab183283.html). We started by tying to optimize both antigens independently first and had success with the Ki-67 staining (primary rat anti-ki67, eBioscience) with the abcam anti-rat secondary HRP polymer and DAB from the kit. However, we still haven?t had any luck with the caspase-3 (primary rabbit anti-caspase 3, cell signaling & abcam anti-rabbit secondary AP polymer and permanent red). We also tried the caspase-3 staining in cell culture after treating cells with an apoptosis inducer, and only saw extremely weak staining. In the FFPE sections, we have used overnight antigen retrieval in citrate buffer ph 6, at 60 C. In both the cell and FFPE sections we do an overnight primary antibody incubation at 4C. We haven?t seen any convincing staining in our FFPE sections (there is some dark pink staining but only in one or two bone marrow cells per section and it seems pretty diffuse) and also have noticed a yellow/brown precipitate forming and only showing up in the bone marrow, it also shows up in our negative antibody control sections. We also tried several different chromogen development times ranging from 10 min to an hour, and didn?t really see a difference. All sections are of course deparaffinized in xylenes and rehydrated in ethanols before starting. All washes are done with TBS-T and sections are permeabilized in 0.1% Triton X100 and blocked in 1%BSA in TBST before primary incubation. All sections are blocked with BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution from Vector after primary incubation, but before secondary incubation. If anyone has any insight into caspase-3 antibodies or doing IHC double staining that would be great! Many thanks, Melanie -- Melanie Smith, MS Research Technician II Department of Biomedical Engineering University of Delaware melsmith@udel.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Wed Oct 8 10:56:25 2014 From: JWatson <@t> gnf.org (James Watson) Date: Wed Oct 8 10:56:31 2014 Subject: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 In-Reply-To: References: Message-ID: Sorry forgot to add loner HIER damaged the Caspase 3 antigen. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, October 08, 2014 8:52 AM To: Melanie Smith; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 Melanie, We do this stain, we run Ventana stainers (no advertisement for Ventana intended) . We had to do this as a sequential double stain since the Caspase 3 needed polymer detection where the Ki67 only needs ABC detection. Caspase 3 was done first. Some other differences: Caspase 3 needed FC receptor block and permeablization. Ki67 needed longer HIER time than Caspase 3 so we did a second short HIER after the Caspase 3 was labeled with DAB. Jamie James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melanie Smith Sent: Wednesday, October 08, 2014 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 Hi everyone, My lab is trying to do caspase-3 and Ki67 double IHC stain on mouse ffpe knee joint sections, and we're having issues optimizing the caspase-3 staining. We originally tried to do fluorescent labels but had too much autofluorescence even after trying several methods of knocking it down. We decided to move on to traditional IHC and purchased a double stain IHC kit from abcam R&Rt on human/mouse tissue (DAB & AP/Red) ( http://www.abcam.com/doublestain-ihc-kit-ramprt-on-humanmouse-tissue-dab-amp-apred-ab183283.html). We started by tying to optimize both antigens independently first and had success with the Ki-67 staining (primary rat anti-ki67, eBioscience) with the abcam anti-rat secondary HRP polymer and DAB from the kit. However, we still haven?t had any luck with the caspase-3 (primary rabbit anti-caspase 3, cell signaling & abcam anti-rabbit secondary AP polymer and permanent red). We also tried the caspase-3 staining in cell culture after treating cells with an apoptosis inducer, and only saw extremely weak staining. In the FFPE sections, we have used overnight antigen retrieval in citrate buffer ph 6, at 60 C. In both the cell and FFPE sections we do an overnight primary antibody incubation at 4C. We haven?t seen any convincing staining in our FFPE sections (there is some dark pink staining but only in one or two bone marrow cells per section and it seems pretty diffuse) and also have noticed a yellow/brown precipitate forming and only showing up in the bone marrow, it also shows up in our negative antibody control sections. We also tried several different chromogen development times ranging from 10 min to an hour, and didn?t really see a difference. All sections are of course deparaffinized in xylenes and rehydrated in ethanols before starting. All washes are done with TBS-T and sections are permeabilized in 0.1% Triton X100 and blocked in 1%BSA in TBST before primary incubation. All sections are blocked with BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution from Vector after primary incubation, but before secondary incubation. If anyone has any insight into caspase-3 antibodies or doing IHC double staining that would be great! Many thanks, Melanie -- Melanie Smith, MS Research Technician II Department of Biomedical Engineering University of Delaware melsmith@udel.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV <@t> mail.amc.edu Wed Oct 8 11:47:54 2014 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Wed Oct 8 11:48:09 2014 Subject: [Histonet] RE: Symphony wicking In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC0112552B23@JERRY.Gia.com> References: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org>, <5A33C952BB67F4468AF1F36D739212BC0112552B23@JERRY.Gia.com> Message-ID: We have a combination of things that we do. Most people cut their slides and stand them up on the side of their waterbath until they are dry and then put them in a rack and transfer them to the Symphony slide tray. If there is any remaining water on the slide they wipe that off with a Kim Wipe before putting in the tray. Vicki Gauch AMCH Albany, NY ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mckenzie@gastrodocs.net] Sent: Wednesday, October 08, 2014 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Symphony wicking For those of you who have a Symphony, how do you wick your slides? Do you cut them and put them in a basket to drain and then transfer to the trays? Do you cut them and lean them up against your water bath? Do you out them in the oven for a few minutes before you load them onto the symphony? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From suetp918 <@t> comcast.net Wed Oct 8 11:49:14 2014 From: suetp918 <@t> comcast.net (Sue) Date: Wed Oct 8 11:49:32 2014 Subject: [Histonet] Symphony wicking In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC0112552B23@JERRY.Gia.com> References: <24C4B3C167E5694887AB594C7602CE3A0D0D62@WN35104.or.providence.org> <5A33C952BB67F4468AF1F36D739212BC0112552B23@JERRY.Gia.com> Message-ID: <733085896.908920.1412786954000.JavaMail.root@comcast.net> Most of my staff put them against their waterbath or hold them in?a tray for some time.? For the tech that?place them directly on the trays we usually angel the tray and jut use a kimwipe to remove the water at the bottom of the slide before putting it on the stainer.? We do not place them in an oven prior to staining mostly due to process and oven space.? But it really is what ever works for each institution. ? Sue Paturzo TJUH From kalwine <@t> wellspan.org Wed Oct 8 12:29:30 2014 From: kalwine <@t> wellspan.org (Alwine, Kristin) Date: Wed Oct 8 12:30:01 2014 Subject: [Histonet] abnormal liver control tissue Message-ID: <829c94904fbb42819e8eb7440fdc5117@BLUPR02MB504.namprd02.prod.outlook.com> We are in need of abnormal liver tissue for our PAS with and without digestion controls. We are looking for tissue from a patient deficient in alpha-1-antitrypsin. If you have any tissue to spare, please let me know! Thank you, Kristin Alwine MT, HTL (ASCP) York Hospital, York PA ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ From Pmcdavid <@t> mhg.com Wed Oct 8 14:27:26 2014 From: Pmcdavid <@t> mhg.com (Patti McDavid) Date: Wed Oct 8 14:27:31 2014 Subject: [Histonet] RE: Dragon and Cerner Message-ID: <1F886A89F4A53345928A11203C028957D7ADB869@JANDC600-MBN02.mhg.local> We went live with Cerner in mid June. Our PA and Patholgists are using Dragon and making their own templates and shortcuts through Dragon. No problems here. Patti McDavid Memorial Hospital Gulfport -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, October 08, 2014 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 131, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Kim's question - order documentation (Cheryl) 2. RE: Kim's question - order documentation (Martha Ward-Pathology) 3. RE: [EXTERNAL] RE: [Histonet] Kim's question - order documentation (Kolman, Kimberly D.) 4. Sakura VIP Tissue Processor (Adesupo, Adesuyi (Banjo)) 5. Dragon and Cerner (Mike Pence) 6. Symphony wicking (Amber McKenzie) 7. Re: Dragon and Cerner (Sue) 8. RE: Dragon and Cerner (Weems, Joyce K.) 9. RE: Symphony wicking (Cooper, Brian) 10. Mouse FFPE IHC double stain caspase-3 and Ki67 (Melanie Smith) 11. RE: Mouse FFPE IHC double stain caspase-3 and Ki67 (James Watson) 12. RE: Mouse FFPE IHC double stain caspase-3 and Ki67 (James Watson) 13. RE: Symphony wicking (Gauch, Vicki) 14. Re: Symphony wicking (Sue) ---------------------------------------------------------------------- Message: 1 Date: Tue, 7 Oct 2014 10:13:31 -0700 From: Cheryl Subject: [Histonet] Kim's question - order documentation To: "histonet@lists.utsouthwestern.edu" Message-ID: <1412702011.76602.YahooMailNeo@web161202.mail.bf1.yahoo.com> Content-Type: text/plain; charset=us-ascii Kim- Your histonet question may not be as complicated as it might seem. Sometimes it's easier to look at these things backwards. What is the desired outcome? If there is an order -- say for a GMS--and it wasn't ordered by one of your pathologists, where did it come from? Can you track back and figure out what doctor ordered it and verify it's a valid request so the testing AND billing is appropriate (not fraudulent). When the surgeon or clinician collects the sample at the surgery or in their office, sometimes they want something specific -- say 'evaluate for fungus'. They may include this in the surgical notes, the office chart -- other places. His support staff will copy this onto the requisition or somewhere you get the request other than the requisition. If you keep copies of the req and other incoming documentation-- you've satisfied the requirement--you can track the source of the order. If you don't, include it in the gross description or notes that are transcribed onto the report so that you have a durable record that you can find (may take a while if it's the archived chart, but you can find it). This goes back to the requirement that orders can't just come from anyone or for any wild-hair reason-- and you have to be able to substantiate or prove the valid source of an ordered (and billed) test. Does that help? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab - one Great Tech at a time. 281.852.9457 Office 800.756.3309 Phone and Fax admin@fullstaff.org ------------------------------ Message: 2 Date: Tue, 7 Oct 2014 19:02:39 +0000 From: Martha Ward-Pathology Subject: RE: [Histonet] Kim's question - order documentation To: Cheryl , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I think this is an interesting question. We frequently get phone calls from clinicians asking for ER, PR, Her2 or sometimes just other IHC stains....just yesterday someone wanted CYK 7 and CYK 20 on a cytology block. We ask that they either call the pathologist who signed out the case and get them to order the stains, or with something like the breast panel, ask that they fax or email us, stating exactly what they want, the patient demographics and surgical number, etc. That way at least we have a paper trail for the files should anyone ask why we did the testing. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, October 07, 2014 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kim's question - order documentation Kim- Your histonet question may not be as complicated as it might seem. Sometimes it's easier to look at these things backwards. What is the desired outcome? If there is an order -- say for a GMS--and it wasn't ordered by one of your pathologists, where did it come from? Can you track back and figure out what doctor ordered it and verify it's a valid request so the testing AND billing is appropriate (not fraudulent). When the surgeon or clinician collects the sample at the surgery or in their office, sometimes they want something specific -- say 'evaluate for fungus'. They may include this in the surgical notes, the office chart -- other places. His support staff will copy this onto the requisition or somewhere you get the request other than the requisition. If you keep copies of the req and other incoming documentation-- you've satisfied the requirement--you can track the source of the order. If you don't, include it in the gross description or notes that are transcribed onto the report so that you have a durable record that you can find (may take a while if it's the archived chart, but you can find it). This goes back to the requirement that orders can't just come from anyone or for any wild-hair reason-- and you have to be able to substantiate or prove the valid source of an ordered (and billed) test. Does that help? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab - one Great Tech at a time. 281.852.9457 Office 800.756.3309 Phone and Fax admin@fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 7 Oct 2014 14:33:11 -0500 From: "Kolman, Kimberly D." Subject: RE: [EXTERNAL] RE: [Histonet] Kim's question - order documentation To: Martha Ward-Pathology , Cheryl , Message-ID: <9C32F30B6662D74A8419DDDB7E66656A091E8662@VHAV15MSGA1.v15.med.va.gov> Content-Type: text/plain; charset="iso-8859-1" Ok I guess I'm coming from a different world; so many things, like adequacy on an FNA, FS or the like are a given, as are ER, PR, etc on tumors. We have access to patient history here so are able to get a more thorough picture of what a clinician is looking for. Of course these are all addressed in the report. Clinicians asking for something 'wild-hair' are not the last word; our pathologists have the final say on what testing may or may not be done. If the clinicians' request is not honored, I don't see the reason for noting it. I think we are well covered with our current practice. Just have to hope CAP thinks so as well.............. :) Thanks everyone! Kim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, October 07, 2014 2:03 PM To: Cheryl; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: [Histonet] Kim's question - order documentation I think this is an interesting question. We frequently get phone calls from clinicians asking for ER, PR, Her2 or sometimes just other IHC stains....just yesterday someone wanted CYK 7 and CYK 20 on a cytology block. We ask that they either call the pathologist who signed out the case and get them to order the stains, or with something like the breast panel, ask that they fax or email us, stating exactly what they want, the patient demographics and surgical number, etc. That way at least we have a paper trail for the files should anyone ask why we did the testing. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, October 07, 2014 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kim's question - order documentation Kim- Your histonet question may not be as complicated as it might seem. Sometimes it's easier to look at these things backwards. What is the desired outcome? If there is an order -- say for a GMS--and it wasn't ordered by one of your pathologists, where did it come from? Can you track back and figure out what doctor ordered it and verify it's a valid request so the testing AND billing is appropriate (not fraudulent). When the surgeon or clinician collects the sample at the surgery or in their office, sometimes they want something specific -- say 'evaluate for fungus'. They may include this in the surgical notes, the office chart -- other places. His support staff will copy this onto the requisition or somewhere you get the request other than the requisition. If you keep copies of the req and other incoming documentation-- you've satisfied the requirement--you can track the source of the order. If you don't, include it in the gross description or notes that are transcribed onto the report so that you have a durable record that you can find (may take a while if it's the archived chart, but you can find it). This goes back to the requirement that orders can't just come from anyone or for any wild-hair reason-- and you have to be able to substantiate or prove the valid source of an ordered (and billed) test. Does that help? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab - one Great Tech at a time. 281.852.9457 Office 800.756.3309 Phone and Fax admin@fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 7 Oct 2014 16:32:11 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] Sakura VIP Tissue Processor To: "histonet@lists.utsouthwestern.edu" Message-ID: <04EE4F75BB5FB246ADB68D69B74604439468E27921@MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="iso-8859-1" Hi, Please i will appreciate it if you guys can tell me which one of the Sakura VIP Tissue Processors is the best. We are trying to get a new tissue processor and we don't want to make any mistake this time around. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor, Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 5 Date: Wed, 8 Oct 2014 14:20:01 +0000 From: Mike Pence Subject: [Histonet] Dragon and Cerner To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hey everyone, Just wondering who out there is successfully using Dragon within the AP application of Cerner? Does it work? Not work? Are you using templates in Dragon or Cerner? You can PM me if you like. This is a peer benchmarking I would like to compare what we do now to what other Cerner users are doing. I don't want to ask this question in a Cerner forum where Cerner can control the commentary. Thanks, Mike Pence AP Supervisor ------------------------------ Message: 6 Date: Wed, 8 Oct 2014 14:24:35 +0000 From: Amber McKenzie Subject: [Histonet] Symphony wicking To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BC0112552B23@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" For those of you who have a Symphony, how do you wick your slides? Do you cut them and put them in a basket to drain and then transfer to the trays? Do you cut them and lean them up against your water bath? Do you out them in the oven for a few minutes before you load them onto the symphony? Thanks! ------------------------------ Message: 7 Date: Wed, 8 Oct 2014 14:29:05 +0000 (UTC) From: Sue Subject: Re: [Histonet] Dragon and Cerner To: Mike Pence Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: <2115505733.889404.1412778545299.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 We use Dragon with CoPath Plus and works great, you can make macros and I know my PA have created some quick texts as well.?? We actually stared with Kurzweil in the 80's and expanded to Dragon speak.?? the only time we do not use Dragon is with Synoptic, although it works, it is labor intensive. ?? Susan T. Paturzo TJUH ------------------------------ Message: 8 Date: Wed, 8 Oct 2014 14:36:20 +0000 From: "Weems, Joyce K." Subject: [Histonet] RE: Dragon and Cerner To: "'Mike Pence'" , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We use it successfully for micro. We found there was too much noise and muffling in the background for gross. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, October 08, 2014 10:20 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Dragon and Cerner Hey everyone, Just wondering who out there is successfully using Dragon within the AP application of Cerner? Does it work? Not work? Are you using templates in Dragon or Cerner? You can PM me if you like. This is a peer benchmarking I would like to compare what we do now to what other Cerner users are doing. I don't want to ask this question in a Cerner forum where Cerner can control the commentary. Thanks, Mike Pence AP Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 9 Date: Wed, 8 Oct 2014 14:50:50 +0000 From: "Cooper, Brian" Subject: RE: [Histonet] Symphony wicking To: "histonet@lists.utsouthwestern.edu" , "amber.mckenzie@gastrodocs.net" Message-ID: Content-Type: text/plain; charset=us-ascii We put our slides into the Symphony trays, then bake them at 75 degrees for 15 minutes in our incubators. Then we stain with our routine H&E program, which omits baking inside the Symphony. It's faster than having a bottleneck of slides stack up in the Symphony's intellique, awaiting baking. Brian Thanks, Brian Cooper, HT (ASCP) Histology Supervisor, Path & Lab Medicine Children's Hospital, Los Angeles Sent from my Galaxy S3, so please forgive any weird typos . . . -----Original Message----- From: Amber McKenzie [amber.mckenzie@gastrodocs.net] Received: Wednesday, 08 Oct 2014, 7:27AM To: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu] Subject: [Histonet] Symphony wicking For those of you who have a Symphony, how do you wick your slides? Do you cut them and put them in a basket to drain and then transfer to the trays? Do you cut them and lean them up against your water bath? Do you out them in the oven for a few minutes before you load them onto the symphony? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ------------------------------ Message: 10 Date: Wed, 8 Oct 2014 11:38:01 -0400 From: Melanie Smith Subject: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi everyone, My lab is trying to do caspase-3 and Ki67 double IHC stain on mouse ffpe knee joint sections, and we're having issues optimizing the caspase-3 staining. We originally tried to do fluorescent labels but had too much autofluorescence even after trying several methods of knocking it down. We decided to move on to traditional IHC and purchased a double stain IHC kit from abcam R&Rt on human/mouse tissue (DAB & AP/Red) ( http://www.abcam.com/doublestain-ihc-kit-ramprt-on-humanmouse-tissue-dab-amp-apred-ab183283.html). We started by tying to optimize both antigens independently first and had success with the Ki-67 staining (primary rat anti-ki67, eBioscience) with the abcam anti-rat secondary HRP polymer and DAB from the kit. However, we still haven???t had any luck with the caspase-3 (primary rabbit anti-caspase 3, cell signaling & abcam anti-rabbit secondary AP polymer and permanent red). We also tried the caspase-3 staining in cell culture after treating cells with an apoptosis inducer, and only saw extremely weak staining. In the FFPE sections, we have used overnight antigen retrieval in citrate buffer ph 6, at 60 C. In both the cell and FFPE sections we do an overnight primary antibody incubation at 4C. We haven???t seen any convincing staining in our FFPE sections (there is some dark pink staining but only in one or two bone marrow cells per section and it seems pretty diffuse) and also have noticed a yellow/brown precipitate forming and only showing up in the bone marrow, it also shows up in our negative antibody control sections. We also tried several different chromogen development times ranging from 10 min to an hour, and didn???t really see a difference. All sections are of course deparaffinized in xylenes and rehydrated in ethanols before starting. All washes are done with TBS-T and sections are permeabilized in 0.1% Triton X100 and blocked in 1%BSA in TBST before primary incubation. All sections are blocked with BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution from Vector after primary incubation, but before secondary incubation. If anyone has any insight into caspase-3 antibodies or doing IHC double staining that would be great! Many thanks, Melanie -- Melanie Smith, MS Research Technician II Department of Biomedical Engineering University of Delaware melsmith@udel.edu ------------------------------ Message: 11 Date: Wed, 8 Oct 2014 15:52:17 +0000 From: James Watson Subject: RE: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 To: Melanie Smith , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" Melanie, We do this stain, we run Ventana stainers (no advertisement for Ventana intended) . We had to do this as a sequential double stain since the Caspase 3 needed polymer detection where the Ki67 only needs ABC detection. Caspase 3 was done first. Some other differences: Caspase 3 needed FC receptor block and permeablization. Ki67 needed longer HIER time than Caspase 3 so we did a second short HIER after the Caspase 3 was labeled with DAB. Jamie James Watson HT?? ASCP GNF?? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel?????? 858-332-4647 Fax???? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melanie Smith Sent: Wednesday, October 08, 2014 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 Hi everyone, My lab is trying to do caspase-3 and Ki67 double IHC stain on mouse ffpe knee joint sections, and we're having issues optimizing the caspase-3 staining. We originally tried to do fluorescent labels but had too much autofluorescence even after trying several methods of knocking it down. We decided to move on to traditional IHC and purchased a double stain IHC kit from abcam R&Rt on human/mouse tissue (DAB & AP/Red) ( http://www.abcam.com/doublestain-ihc-kit-ramprt-on-humanmouse-tissue-dab-amp-apred-ab183283.html). We started by tying to optimize both antigens independently first and had success with the Ki-67 staining (primary rat anti-ki67, eBioscience) with the abcam anti-rat secondary HRP polymer and DAB from the kit. However, we still haven???t had any luck with the caspase-3 (primary rabbit anti-caspase 3, cell signaling & abcam anti-rabbit secondary AP polymer and permanent red). We also tried the caspase-3 staining in cell culture after treating cells with an apoptosis inducer, and only saw extremely weak staining. In the FFPE sections, we have used overnight antigen retrieval in citrate buffer ph 6, at 60 C. In both the cell and FFPE sections we do an overnight primary antibody incubation at 4C. We haven???t seen any convincing staining in our FFPE sections (there is some dark pink staining but only in one or two bone marrow cells per section and it seems pretty diffuse) and also have noticed a yellow/brown precipitate forming and only showing up in the bone marrow, it also sho ws up in our negative antibody control sections. We also tried several different chromogen development times ranging from 10 min to an hour, and didn???t really see a difference. All sections are of course deparaffinized in xylenes and rehydrated in ethanols before starting. All washes are done with TBS-T and sections are permeabilized in 0.1% Triton X100 and blocked in 1%BSA in TBST before primary incubation. All sections are blocked with BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution from Vector after primary incubation, but before secondary incubation. If anyone has any insight into caspase-3 antibodies or doing IHC double staining that would be great! Many thanks, Melanie -- Melanie Smith, MS Research Technician II Department of Biomedical Engineering University of Delaware melsmith@udel.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 8 Oct 2014 15:56:25 +0000 From: James Watson Subject: RE: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 To: James Watson , Melanie Smith , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" Sorry forgot to add loner HIER damaged the Caspase 3 antigen. James Watson HT?? ASCP GNF?? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel?????? 858-332-4647 Fax???? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, October 08, 2014 8:52 AM To: Melanie Smith; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 Melanie, We do this stain, we run Ventana stainers (no advertisement for Ventana intended) . We had to do this as a sequential double stain since the Caspase 3 needed polymer detection where the Ki67 only needs ABC detection. Caspase 3 was done first. Some other differences: Caspase 3 needed FC receptor block and permeablization. Ki67 needed longer HIER time than Caspase 3 so we did a second short HIER after the Caspase 3 was labeled with DAB. Jamie James Watson HT?? ASCP GNF?? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel?????? 858-332-4647 Fax???? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melanie Smith Sent: Wednesday, October 08, 2014 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67 Hi everyone, My lab is trying to do caspase-3 and Ki67 double IHC stain on mouse ffpe knee joint sections, and we're having issues optimizing the caspase-3 staining. We originally tried to do fluorescent labels but had too much autofluorescence even after trying several methods of knocking it down. We decided to move on to traditional IHC and purchased a double stain IHC kit from abcam R&Rt on human/mouse tissue (DAB & AP/Red) ( http://www.abcam.com/doublestain-ihc-kit-ramprt-on-humanmouse-tissue-dab-amp-apred-ab183283.html). We started by tying to optimize both antigens independently first and had success with the Ki-67 staining (primary rat anti-ki67, eBioscience) with the abcam anti-rat secondary HRP polymer and DAB from the kit. However, we still haven???t had any luck with the caspase-3 (primary rabbit anti-caspase 3, cell signaling & abcam anti-rabbit secondary AP polymer and permanent red). We also tried the caspase-3 staining in cell culture after treating cells with an apoptosis inducer, and only saw extremely weak staining. In the FFPE sections, we have used overnight antigen retrieval in citrate buffer ph 6, at 60 C. In both the cell and FFPE sections we do an overnight primary antibody incubation at 4C. We haven???t seen any convincing staining in our FFPE sections (there is some dark pink staining but only in one or two bone marrow cells per section and it seems pretty diffuse) and also have noticed a yellow/brown precipitate forming and only showing up in the bone marrow, it also sho ws up in our negative antibody control sections. We also tried several different chromogen development times ranging from 10 min to an hour, and didn???t really see a difference. All sections are of course deparaffinized in xylenes and rehydrated in ethanols before starting. All washes are done with TBS-T and sections are permeabilized in 0.1% Triton X100 and blocked in 1%BSA in TBST before primary incubation. All sections are blocked with BLOXALL Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution from Vector after primary incubation, but before secondary incubation. If anyone has any insight into caspase-3 antibodies or doing IHC double staining that would be great! Many thanks, Melanie -- Melanie Smith, MS Research Technician II Department of Biomedical Engineering University of Delaware melsmith@udel.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 8 Oct 2014 16:47:54 +0000 From: "Gauch, Vicki" Subject: [Histonet] RE: Symphony wicking To: Amber McKenzie , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have a combination of things that we do. Most people cut their slides and stand them up on the side of their waterbath until they are dry and then put them in a rack and transfer them to the Symphony slide tray. If there is any remaining water on the slide they wipe that off with a Kim Wipe before putting in the tray. Vicki Gauch AMCH Albany, NY ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mckenzie@gastrodocs.net] Sent: Wednesday, October 08, 2014 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Symphony wicking For those of you who have a Symphony, how do you wick your slides? Do you cut them and put them in a basket to drain and then transfer to the trays? Do you cut them and lean them up against your water bath? Do you out them in the oven for a few minutes before you load them onto the symphony? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ------------------------------ Message: 14 Date: Wed, 8 Oct 2014 16:49:14 +0000 (UTC) From: Sue Subject: Re: [Histonet] Symphony wicking To: Amber McKenzie Cc: histonet@lists.utsouthwestern.edu Message-ID: <733085896.908920.1412786954000.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 Most of my staff put them against their waterbath or hold them in??a tray for some time.?? For the tech that??place them directly on the trays we usually angel the tray and jut use a kimwipe to remove the water at the bottom of the slide before putting it on the stainer.?? We do not place them in an oven prior to staining mostly due to process and oven space.?? But it really is what ever works for each institution. ?? Sue Paturzo TJUH ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 131, Issue 9 **************************************** [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **********PRIVATE AND CONFIDENTIAL********** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. From HornHV <@t> archildrens.org Wed Oct 8 14:44:54 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Oct 8 14:45:06 2014 Subject: [Histonet] RE: New CPT Codes for IHC In-Reply-To: References: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> <004201cfe19c$b17c12c0$14743840$@cogipath.com> Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFF61@EVS1.archildrens.org> I would like the correct answer for this. I have always billed as Joyce states. But a sister hospital says it's like Andrew's email. Who is right? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, October 06, 2014 3:41 PM To: 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Sorry, I left that part out. We don't do them, so I just didn't think. I understand the 88343 codes to be for multiple stains on the same slide. The first is 88342 additional 88343 - e.g. PIN4. So has that changed? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Andrew Horvath [mailto:ahorvath@cogipath.com] Sent: Monday, October 06, 2014 3:36 PM To: Weems, Joyce K.; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Actually, for non-Medicare if there are multiple stains performed on a block, where the initial CPT is 88342, any subsequent billing for stains on that block would actually be 88343 from the information I see. Please refer to the links below for more information... http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf https://www.aapc.com/memberarea/forums/showthread.php?t=101402 quote from the forum: Quite and uproar about this in our office - and a lot of confusion. Go to page 360 in this document to read Medicare's decision about this. (inactive link removed) "The CPT Editorial Panel revised the existing immunohistochemistry code, CPT code 88342 and created a new add-on code 88343 for CY 2014. Current coding requirements only allow CPT code 88342 to be billed once per specimen for each antibody, but the revised CPT codes and descriptors would allow the reporting of multiple units for each slide and each block per antibody (88342 for the first antibody and 88343 for subsequent antibodies). We believe that this coding would encourage overutilization by allowing multiple blocks and slides to be billed. To avoid this incentive, we are creating G0461 (Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain) and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (List separately in addition to code for primary procedure)) to ensure that the services are only reported once for each antibody per specimen. We believe this will result in appropriate values for these services without creating incentives for overutilization." Some of our docs are participating in conference call today with CAP to help clarify what this all means. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, October 03, 2014 12:33 PM To: 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CPT Codes for IHC For non-Medicare it is per block - CPT code 88342. For Medicare - per specimen. G0461 for the first antibody and G0462 for each additional different ab. Best, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Friday, October 03, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CPT Codes for IHC Hi, I hope you guys are doing well. Please I have a question about billing the IHC. My question is do we bill by the number of antibodies or blocks? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. 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From lblazek <@t> digestivespecialists.com Wed Oct 8 14:51:17 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Oct 8 14:51:28 2014 Subject: [Histonet] RE: New CPT Codes for IHC In-Reply-To: <25A4DE08332B19499904459F00AAACB719E29EFF61@EVS1.archildrens.org> References: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> <004201cfe19c$b17c12c0$14743840$@cogipath.com> <25A4DE08332B19499904459F00AAACB719E29EFF61@EVS1.archildrens.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E3916997043B5@IBMB7Exchange.digestivespecialists.com> Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, October 08, 2014 3:45 PM To: 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC I would like the correct answer for this. I have always billed as Joyce states. But a sister hospital says it's like Andrew's email. Who is right? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, October 06, 2014 3:41 PM To: 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Sorry, I left that part out. We don't do them, so I just didn't think. I understand the 88343 codes to be for multiple stains on the same slide. The first is 88342 additional 88343 - e.g. PIN4. So has that changed? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Andrew Horvath [mailto:ahorvath@cogipath.com] Sent: Monday, October 06, 2014 3:36 PM To: Weems, Joyce K.; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Actually, for non-Medicare if there are multiple stains performed on a block, where the initial CPT is 88342, any subsequent billing for stains on that block would actually be 88343 from the information I see. Please refer to the links below for more information... http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf https://www.aapc.com/memberarea/forums/showthread.php?t=101402 quote from the forum: Quite and uproar about this in our office - and a lot of confusion. Go to page 360 in this document to read Medicare's decision about this. (inactive link removed) "The CPT Editorial Panel revised the existing immunohistochemistry code, CPT code 88342 and created a new add-on code 88343 for CY 2014. Current coding requirements only allow CPT code 88342 to be billed once per specimen for each antibody, but the revised CPT codes and descriptors would allow the reporting of multiple units for each slide and each block per antibody (88342 for the first antibody and 88343 for subsequent antibodies). We believe that this coding would encourage overutilization by allowing multiple blocks and slides to be billed. To avoid this incentive, we are creating G0461 (Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain) and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (List separately in addition to code for primary procedure)) to ensure that the services are only reported once for each antibody per specimen. We believe this will result in appropriate values for these services without creating incentives for overutilization." Some of our docs are participating in conference call today with CAP to help clarify what this all means. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, October 03, 2014 12:33 PM To: 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CPT Codes for IHC For non-Medicare it is per block - CPT code 88342. For Medicare - per specimen. G0461 for the first antibody and G0462 for each additional different ab. Best, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Friday, October 03, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CPT Codes for IHC Hi, I hope you guys are doing well. Please I have a question about billing the IHC. My question is do we bill by the number of antibodies or blocks? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From millers <@t> emhs.org Wed Oct 8 15:33:43 2014 From: millers <@t> emhs.org (Miller, Suzie) Date: Wed Oct 8 15:33:52 2014 Subject: [Histonet] RE: New CPT Codes for IHC In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3916997043B5@IBMB7Exchange.digestivespecialists.com> References: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> <004201cfe19c$b17c12c0$14743840$@cogipath.com> <25A4DE08332B19499904459F00AAACB719E29EFF61@EVS1.archildrens.org>, <5A2BD13465E061429D6455C8D6B40E3916997043B5@IBMB7Exchange.digestivespecialists.com> Message-ID: We also interpret the coding to be as stated below by Joyce. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Blazek, Linda [lblazek@digestivespecialists.com] Sent: Wednesday, October 08, 2014 3:51 PM To: Horn, Hazel V; 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, October 08, 2014 3:45 PM To: 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC I would like the correct answer for this. I have always billed as Joyce states. But a sister hospital says it's like Andrew's email. Who is right? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, October 06, 2014 3:41 PM To: 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Sorry, I left that part out. We don't do them, so I just didn't think. I understand the 88343 codes to be for multiple stains on the same slide. The first is 88342 additional 88343 - e.g. PIN4. So has that changed? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Andrew Horvath [mailto:ahorvath@cogipath.com] Sent: Monday, October 06, 2014 3:36 PM To: Weems, Joyce K.; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Actually, for non-Medicare if there are multiple stains performed on a block, where the initial CPT is 88342, any subsequent billing for stains on that block would actually be 88343 from the information I see. Please refer to the links below for more information... http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf https://www.aapc.com/memberarea/forums/showthread.php?t=101402 quote from the forum: Quite and uproar about this in our office - and a lot of confusion. Go to page 360 in this document to read Medicare's decision about this. (inactive link removed) "The CPT Editorial Panel revised the existing immunohistochemistry code, CPT code 88342 and created a new add-on code 88343 for CY 2014. Current coding requirements only allow CPT code 88342 to be billed once per specimen for each antibody, but the revised CPT codes and descriptors would allow the reporting of multiple units for each slide and each block per antibody (88342 for the first antibody and 88343 for subsequent antibodies). We believe that this coding would encourage overutilization by allowing multiple blocks and slides to be billed. To avoid this incentive, we are creating G0461 (Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain) and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (List separately in addition to code for primary procedure)) to ensure that the services are only reported once for each antibody per specimen. We believe this will result in appropriate values for these services without creating incentives for overutilization." Some of our docs are participating in conference call today with CAP to help clarify what this all means. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, October 03, 2014 12:33 PM To: 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CPT Codes for IHC For non-Medicare it is per block - CPT code 88342. For Medicare - per specimen. G0461 for the first antibody and G0462 for each additional different ab. Best, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Friday, October 03, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CPT Codes for IHC Hi, I hope you guys are doing well. Please I have a question about billing the IHC. My question is do we bill by the number of antibodies or blocks? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------------- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. From WaitT <@t> livemail.uthscsa.edu Wed Oct 8 16:09:58 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Wed Oct 8 16:10:06 2014 Subject: [Histonet] Decalcified Bone Staining Problems Message-ID: <1412802600334.68811@livemail.uthscsa.edu> Hey guys! I was curious if anyone could send me a link or maybe even just a source where I could see the difference between a properly/completely decalcified H&E stained bone tissue vs. an improper/incompletely stained decalcified specimen. This would be beneficial because I believe that the tissues that I am currently staining with H&E are not totally decalcifed, therefore there are sections in the bone tissue that are stained a bit more heavily than others...and this pattern of stains holds pretty consistently with the all of the sections that were taken from one specific bone block. In short, I would just love to see what a quality stained uniform H&E section looked like. Here are several possible explanations as to why my slides are not staining uniformly: - Perhaps the way I am letting the slides dry? - when the slides are completely done staining and clearing, I let them dry and perhaps that is something to do with it. - I am not putting coverslips on the slides yet because I just want to get the staining protocol down first...perhaps if I put the coverslips on then that would help solve the problem? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry From pdefazio802 <@t> gmail.com Wed Oct 8 17:15:22 2014 From: pdefazio802 <@t> gmail.com (Pam DeFazio) Date: Wed Oct 8 17:15:27 2014 Subject: [Histonet] Grossing blades Message-ID: Our pathologists use the double edge blades (Pathco) and recently have been complaining about them being dull. Anyone else experiencing this? From doug.porter <@t> caplab.org Wed Oct 8 17:40:04 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Wed Oct 8 17:35:35 2014 Subject: [Histonet] Grossing blades In-Reply-To: References: Message-ID: <008e01cfe348$cecf8250$6c6e86f0$@caplab.org> I have experienced the same issue off and on. I think there are bad lot numbers on occasion. Perhaps you could get them to exchange them for a different lot? I am also suspect that some companies have started using inferior metal that just won't hold an edge as long. Just like microtome blades, sometimes you just get a bad box. Good luck and happy cutting. Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow / CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam DeFazio Sent: Wednesday, October 08, 2014 6:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing blades Our pathologists use the double edge blades (Pathco) and recently have been complaining about them being dull. Anyone else experiencing this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4765 / Virus Database: 4037/8345 - Release Date: 10/07/14 From rsrichmond <@t> gmail.com Wed Oct 8 17:45:52 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Oct 8 17:45:55 2014 Subject: [Histonet] liver in alpha-1 antitrypsin deficiency (was abnormal liver tissue) Message-ID: Kristin Alwine MT, HTL (ASCP) at York Hospital, York PA asks: >>We are in need of abnormal liver tissue for our PAS with and without digestion controls. We are looking for tissue from a patient deficient in alpha-1-antitrypsin. If you have any tissue to spare, please let me know!<< Somebody must have this in good quantity, since patients with antitrypsin deficiency occasionally undergo liver transplants. University of Pittsburgh might be a good place to start. I'd be content with normal liver as a control, though. In the one case I've ever seen, the PAS positive granules were quite easy to see, in a middle aged man presenting with cirrhosis whom my client had diagnosed as having "alcoholic cirrhosis" (which is not a legitimate pathologic diagnosis). The patient was a Baptist preacher who could easily have lost his job, and he was quite angry. His internist ordered all tests until positive, and the man turned out to be heterozygous for deficient AAT (the MZ genotype, I suppose). The PAS-diastase stain on the biopsy quickly showed the granules. Bob Richmond Samurai Pathologist Maryville TN From chesarato <@t> hotmail.com Wed Oct 8 20:31:15 2014 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Wed Oct 8 20:31:20 2014 Subject: [Histonet] Re: Decalcified Bone Staining Problems. Message-ID: In the link below ( Figure 5 ) Incomplete decalcified Bone: Basophilic and Brittle. http://www.dentalaegis.com/cced/2011/03/alveolar-ridge-augmentation-comparion-of-two-socket-graft-materials-in-implant-cases In the link below Complete Decalcified Bone: Pink and with well preserved morphology. http://www.visualphotos.com/image/1x8802590/decalcified_compact_bones Cesar RomeroBuenos AiresArgentina From carl.hobbs <@t> kcl.ac.uk Wed Oct 8 21:51:29 2014 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Oct 8 21:51:38 2014 Subject: [Histonet] re:Mouse FFPE IHC double stain caspase-3 and Ki67 (Melanie Smith) Message-ID: <1412823089456.71741@kcl.ac.uk> Firstly, take a piece of tissue that clearly shows Ki67/CC3, such as small intestine. Do your std fix and processing. Also.....take another piece, fix and decalcify identically to your kneejoints. Then process the same way. Optimise both Abs independently, on the NON-decalcified gut sections. Once optimised?, repeat on the decalcified gut. If the latter works.....then so should double-labelling then...your kneejoints! Does this make sense? Best regards carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From mward <@t> wakehealth.edu Thu Oct 9 07:37:06 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Oct 9 07:37:15 2014 Subject: [Histonet] RE: New CPT Codes for IHC In-Reply-To: References: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> <004201cfe19c$b17c12c0$14743840$@cogipath.com> <25A4DE08332B19499904459F00AAACB719E29EFF61@EVS1.archildrens.org>, <5A2BD13465E061429D6455C8D6B40E3916997043B5@IBMB7Exchange.digestivespecialists.com> Message-ID: We do our billing as Joyce does as well. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miller, Suzie Sent: Wednesday, October 08, 2014 4:34 PM To: Blazek, Linda; Horn, Hazel V; 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC We also interpret the coding to be as stated below by Joyce. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Blazek, Linda [lblazek@digestivespecialists.com] Sent: Wednesday, October 08, 2014 3:51 PM To: Horn, Hazel V; 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, October 08, 2014 3:45 PM To: 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC I would like the correct answer for this. I have always billed as Joyce states. But a sister hospital says it's like Andrew's email. Who is right? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, October 06, 2014 3:41 PM To: 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Sorry, I left that part out. We don't do them, so I just didn't think. I understand the 88343 codes to be for multiple stains on the same slide. The first is 88342 additional 88343 - e.g. PIN4. So has that changed? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Andrew Horvath [mailto:ahorvath@cogipath.com] Sent: Monday, October 06, 2014 3:36 PM To: Weems, Joyce K.; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Actually, for non-Medicare if there are multiple stains performed on a block, where the initial CPT is 88342, any subsequent billing for stains on that block would actually be 88343 from the information I see. Please refer to the links below for more information... http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf https://www.aapc.com/memberarea/forums/showthread.php?t=101402 quote from the forum: Quite and uproar about this in our office - and a lot of confusion. Go to page 360 in this document to read Medicare's decision about this. (inactive link removed) "The CPT Editorial Panel revised the existing immunohistochemistry code, CPT code 88342 and created a new add-on code 88343 for CY 2014. Current coding requirements only allow CPT code 88342 to be billed once per specimen for each antibody, but the revised CPT codes and descriptors would allow the reporting of multiple units for each slide and each block per antibody (88342 for the first antibody and 88343 for subsequent antibodies). We believe that this coding would encourage overutilization by allowing multiple blocks and slides to be billed. To avoid this incentive, we are creating G0461 (Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain) and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (List separately in addition to code for primary procedure)) to ensure that the services are only reported once for each antibody per specimen. We believe this will result in appropriate values for these services without creating incentives for overutilization." Some of our docs are participating in conference call today with CAP to help clarify what this all means. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, October 03, 2014 12:33 PM To: 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CPT Codes for IHC For non-Medicare it is per block - CPT code 88342. For Medicare - per specimen. G0461 for the first antibody and G0462 for each additional different ab. Best, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Friday, October 03, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CPT Codes for IHC Hi, I hope you guys are doing well. Please I have a question about billing the IHC. My question is do we bill by the number of antibodies or blocks? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------------- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Thu Oct 9 07:42:32 2014 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Thu Oct 9 07:42:40 2014 Subject: [Histonet] RE: New CPT Codes for IHC References: <04EE4F75BB5FB246ADB68D69B74604439468DA8826@MAIL.nrhnt.nrh-ok.com> <004201cfe19c$b17c12c0$14743840$@cogipath.com> <25A4DE08332B19499904459F00AAACB719E29EFF61@EVS1.archildrens.org>, <5A2BD13465E061429D6455C8D6B40E3916997043B5@IBMB7Exchange.digestivespecialists.com> Message-ID: <77ff679efa3f6749e1edb13467b46596@medicalcenterclinic.com> Here are the descriptions per the 2014 CPT and HCPCS books: ? 88342 ? Immunohistochemistry or immunocytochemistry, each separately identifiable antibody per block, cytologic preparation, or hematologic smear; first separately identifiable antibody per slide ? 88343 ? Each additional separately identifiable antibody per slide (list separately in addition to code from primary procedure) ? G0461 ? Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain ? G0462 ? Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (list separately in addition to code for primary procedure) ? So, if you have a specimen with multiple blocks (A1-A3) and you do a?multiplex?stain?with 3 antibodies on A1 and A3, for 88342/88343 you would bill: A1 - 88342 x 1 and 88343 x 2 A3 - 88342 x 1 and 88343 x 2 ? For G0461 and G0462 the above case in both situations would be billed as follows: A1 and A3 - G0461 x 1 and G0462 x 2 ? If there is a block A and block B and you did the multiplex stain for the G-codes: A - G0461 x 1 and G0462 x 2 B - G0461 x 1 and G0462 x 2 What causes confusion for me is if an immunohistochemical?or?multiplex stain is done on levels.? With the 88342 saying per block in the first part of the description, I feel I cannot bill for immuno stains done on additional levels, but then the second part of the description says first separately identifiable antibody per slide and that's when my brain begins to ooze out of my ears.? It seems a bit contradictory to me.? I'd love to read other's thoughts on this. ? ? Brendal C. Finlay, HT (ASCP) -----Original Message----- From: "Miller, Suzie" To: "Blazek, Linda" , "Horn, Hazel V" , "Weems, Joyce K." , "Andrew Horvath" , "Adesupo, Adesuyi (Banjo)" , histonet@lists.utsouthwestern.edu Date: 10/08/2014 15:33 Subject: RE: [Histonet] RE: New CPT Codes for IHC We also interpret the coding to be as stated below by Joyce. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Blazek, Linda [lblazek@digestivespecialists.com] Sent: Wednesday, October 08, 2014 3:51 PM To: Horn, Hazel V; 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, October 08, 2014 3:45 PM To: 'Weems, Joyce K.'; 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC I would like the correct answer for this. ?I have always billed as Joyce states. ?But a sister hospital says it's like Andrew's email. ?Who is right? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, October 06, 2014 3:41 PM To: 'Andrew Horvath'; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Sorry, I left that part out. We don't do them, so I just didn't think. I understand the 88343 codes to be for multiple stains on the same slide. The first is 88342 additional 88343 - e.g. PIN4. So has that changed? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). ?It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Andrew Horvath [mailto:ahorvath@cogipath.com] Sent: Monday, October 06, 2014 3:36 PM To: Weems, Joyce K.; 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: New CPT Codes for IHC Actually, for non-Medicare if there are multiple stains performed on a block, where the initial CPT is 88342, any subsequent billing for stains on that block would actually be 88343 from the information I see. Please refer to the links below for more information... http://ahsrcm.com/assets/2014-Pathology-CPT-Code-Changes.pdf https://www.aapc.com/memberarea/forums/showthread.php?t=101402 quote from the forum: Quite and uproar about this in our office - and a lot of confusion. Go to page 360 in this document to read Medicare's decision about this. (inactive link removed) "The CPT Editorial Panel revised the existing immunohistochemistry code, CPT code 88342 and created a new add-on code 88343 for CY 2014. Current coding requirements only allow CPT code 88342 to be billed once per specimen for each antibody, but the revised CPT codes and descriptors would allow the reporting of multiple units for each slide and each block per antibody (88342 for the first antibody and 88343 for subsequent antibodies). We believe that this coding would encourage overutilization by allowing multiple blocks and slides to be billed. To avoid this incentive, we are creating G0461 (Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain) and G0462 (Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (List separately in addition to code for primary procedure)) to ensure that the services are only reported once for each antibody per specimen. We believe this will result in appropriate values for these services without creating incentives for overutilization." Some of our docs are participating in conference call today with CAP to help clarify what this all means. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, October 03, 2014 12:33 PM To: 'Adesupo, Adesuyi (Banjo)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: New CPT Codes for IHC For non-Medicare it is per block - CPT code 88342. For Medicare - per specimen. G0461 for the first antibody and G0462 for each additional different ab. Best, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). ?It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Friday, October 03, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New CPT Codes for IHC ?Hi, ? ? ? ?I hope you guys are doing well. Please I have a question about billing the IHC. My question is do we bill by the number of antibodies or blocks? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS ?Histology Supervisor ?Norman Regional Health System, ?Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above.. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------------- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Fawn.Bomar <@t> HalifaxRegional.com Thu Oct 9 07:49:30 2014 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Thu Oct 9 07:49:57 2014 Subject: [Histonet] fixation question Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B976311A@EXCH-2K10.hrhs.com> What is the minimum time recommended for fixing routine endoscopy biopsies before placing them on a short bx run program. Thank you for your help Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From tbraud <@t> holyredeemer.com Thu Oct 9 07:54:28 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Oct 9 07:54:35 2014 Subject: [Histonet] RE: IHC billing In-Reply-To: <20141008212318.B587C1E88DA@trendmess-svr.holyredeemer.local> References: <20141008212318.B587C1E88DA@trendmess-svr.holyredeemer.local> Message-ID: Our institution is billing IHC identical to what Joyce stated. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 3 Date: Wed, 8 Oct 2014 14:44:54 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] RE: New CPT Codes for IHC I would like the correct answer for this. I have always billed as Joyce states. But a sister hospital says it's like Andrew's email. Who is right? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From relia1 <@t> earthlink.net Thu Oct 9 10:28:57 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Oct 9 10:29:03 2014 Subject: [Histonet] RELIA HOT JOB ALERT!! 10-9-2014 EXCITING NEW OPPORTUNITIES NATIONWIDE SOME ARE RELIA EXCLUSIVES!!! Message-ID: <000e01cfe3d5$bea35c70$3bea1550$@earthlink.net> Hi Histonetters!! I hope you are enjoying a beautiful day. How bad could it be? It?s October, It?s Football season, the weather is cooling down and It?s just one more day til the Weekend!! (and for all you ?walker hunters? out there it?s just 3 more days till the season premiere) I am also in a great mood today because I have some exciting new jobs that I am working on. Some of these are RELIA exclusives Most of these offer Sign- On Bonuses and or Relocation Assistance All of these Companies offer excellent compensation, benefits and great environments. If you or anyone you know might be interested in any of these positions contact me. You can reach me by email at relia1@earthlink.net toll free at 866-607-3542 or on my cell at 407-353-5070. Here are the exciting opportunities I am talking about: Mohs Histotech ? Hammond, IN Histotechnician ? Hammond, IN Histo tech ? Chesapeake, VA Histotechnologist ? Philadelphia, PA IHC Tech ? Austin, TX Histotechnician ? Austin, TX Dermpath Histotech ? Fayetteville, AR Pathologists? Assistant ? Long Island, NY All of these opportunities are permanent full time positions. If you are interested please contact me. If you know someone who is interested we also offer a referral reward if we place someone you refer to us. Have a great day and an AWESOME Weekend!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From akbitting <@t> geisinger.edu Thu Oct 9 13:36:00 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Oct 9 13:36:08 2014 Subject: [Histonet] Gram staining Message-ID: <77F52EFAB8B1694B885E277C48FCD0F67BA7FF02@GHSEXMBX1W8K1V.geisinger.edu> Our new Pathologist would like to see more yellow in the background of our Gram stain. We use picric acid-acetone currently, which looks more pink than yellow most times. So I'm wondering if a method using a Tartrazine counterstain would be better. Any thoughts? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From TanyaAbbott <@t> catholichealth.net Thu Oct 9 14:03:47 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Thu Oct 9 14:04:14 2014 Subject: [Histonet] Ebola Message-ID: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Joyce.Weems <@t> emoryhealthcare.org Thu Oct 9 14:21:46 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Oct 9 14:21:54 2014 Subject: [Histonet] RE: Ebola In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> Message-ID: Handle with universal precautions as always is our emphasis. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Thursday, October 09, 2014 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ebola Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From bcooper <@t> chla.usc.edu Thu Oct 9 14:48:21 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Oct 9 14:48:27 2014 Subject: [Histonet] RE: Gram staining In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F67BA7FF02@GHSEXMBX1W8K1V.geisinger.edu> References: <77F52EFAB8B1694B885E277C48FCD0F67BA7FF02@GHSEXMBX1W8K1V.geisinger.edu> Message-ID: We use the McDonald's Gram Stain kit from American Master Tech, which employs the use of a Modified Tartrazine counterstain. Both our docs and techs love our Gram stains . . . Here's the link to the product info. http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=KTMGS Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Thursday, October 09, 2014 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gram staining Our new Pathologist would like to see more yellow in the background of our Gram stain. We use picric acid-acetone currently, which looks more pink than yellow most times. So I'm wondering if a method using a Tartrazine counterstain would be better. Any thoughts? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From JWatson <@t> gnf.org Thu Oct 9 14:52:12 2014 From: JWatson <@t> gnf.org (James Watson) Date: Thu Oct 9 14:52:18 2014 Subject: [Histonet] RE: Ebola In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> Message-ID: Have it delivered in formalin and let it fix completely. "Formalin fixation eliminates the biologic hazard associated with transporting samples containing infectious EBO virus, .................... Thus, formalin fixation provides an adequate noninfectious specimen for EHF diagnosis, ........................." A quote from: Long-Term Disease Surveillance in Bandundu Region, Democratic Republic of the Congo: A Model for Early Detection and Prevention of Ebola Hemorrhagic Fever Ethleen S. Lloyd, and C. J. Peters, et al + Author Affiliations Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization and US Agency for International Development and Ministry of Health, Kinshasa, H?pital G?n?ral de R?f?rence de Kikwit, Kikwit, and Mosango Mission Hospital, Mosango, Democratic Republic of the Congo; M?decins sans Fronti?res?Belgium, Brussels, Belgium + Author Notes ?* Current affiliation: Institut de M?decine Tropicale, Antwerp, Belgium (M.A.B.); M?decins sans Fronti?res?Belgium, N'Djamena, Chad (E.V.); World Health Organization, Kinshasa, Democratic Republic of the Congo (J.K.). Reprints or correspondence: Dr. Pierre E. Rollin, Special Pathogens Branch, NCID/DVRD, Mailstop G-14, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333 (pyr3@cdc.gov). James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, October 09, 2014 12:22 PM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ebola Handle with universal precautions as always is our emphasis. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Thursday, October 09, 2014 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ebola Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Oct 9 15:06:23 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Oct 9 15:06:28 2014 Subject: [Histonet] RE: Ebola In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> Message-ID: I did my postdoc research at USAMRIID on Ebola virus pathogenesis. Our standard fixation protocol for Ebola, Lassa and other BSL4 pathogens was to let them fix in formalin for a minimum of 30 days. The containers of fixed tissue were not allowed out of the P4 containment lab until the 30 day limit was up. Even then we had disinfectant dunk tanks to pass the sealed containers through to get them out of the P4 lab. You can't be too careful with that bugger...just sayin' Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, October 09, 2014 3:52 PM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ebola Have it delivered in formalin and let it fix completely. "Formalin fixation eliminates the biologic hazard associated with transporting samples containing infectious EBO virus, .................... Thus, formalin fixation provides an adequate noninfectious specimen for EHF diagnosis, ........................." A quote from: Long-Term Disease Surveillance in Bandundu Region, Democratic Republic of the Congo: A Model for Early Detection and Prevention of Ebola Hemorrhagic Fever Ethleen S. Lloyd, and C. J. Peters, et al + Author Affiliations Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization and US Agency for International Development and Ministry of Health, Kinshasa, H?pital G?n?ral de R?f?rence de Kikwit, Kikwit, and Mosango Mission Hospital, Mosango, Democratic Republic of the Congo; M?decins sans Fronti?res?Belgium, Brussels, Belgium + Author Notes ?* Current affiliation: Institut de M?decine Tropicale, Antwerp, Belgium (M.A.B.); M?decins sans Fronti?res?Belgium, N'Djamena, Chad (E.V.); World Health Organization, Kinshasa, Democratic Republic of the Congo (J.K.). Reprints or correspondence: Dr. Pierre E. Rollin, Special Pathogens Branch, NCID/DVRD, Mailstop G-14, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333 (pyr3@cdc.gov). James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, October 09, 2014 12:22 PM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ebola Handle with universal precautions as always is our emphasis. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Thursday, October 09, 2014 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ebola Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From mucram11 <@t> comcast.net Thu Oct 9 15:26:16 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Oct 9 15:26:45 2014 Subject: [Histonet] Ebola In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> Message-ID: <1331788667.1679788.1412886376853.JavaMail.root@comcast.net> Take Brett's advise and use that as guidleine.? We don't know as much as we should about these viruses.?? Pam ----- Original Message ----- From: "Tanya Abbott" To: "Histonet" Sent: Thursday, October 9, 2014 2:03:47 PM Subject: [Histonet] Ebola Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph ?610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Thu Oct 9 15:31:10 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Thu Oct 9 15:31:15 2014 Subject: [Histonet] RE: Ebola In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net>, , Message-ID: All samples should be handled with universal precautions assuming they may be infectious, formalin fixation is considered an eliminator of biologic hazard for most things like Ebola, but then there is always CJD that it does not work for. I would not be doing any frozen sections on samples with suspected biologic hazard since they do not get fixed in formalin typically, although you can fix in formalin then rinse it out and infiltrate with sucrose to make fixed frozen sections. Keep calm and use your common sense and vast experiences. Cheers, Patsy Here is a story for you. Back in the late 70's early 80's when we didn't know much about HIV or HPV I worked in a Heme/Path lab at the U. One of the pathologist I worked with was studying genital wort's and lesions getting her samples from the local sexually transmitted disease clinic. This was back in the day when T&B cells had to be done on cell suspensions, frozen sections or smears/touch preps not aldehyde fixed. The antibodies and detection systems did not yet work in FFPE tissues. I prepared unfixed frozen samples on a ton of these biopsies. I double gloved, used UP and got thru this. Looking back on that project I do not think I would have done it knowing what I know now. There is a reason or two that Histotechnology is the most dangerous job I guess. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From: JWatson@gnf.org To: Joyce.Weems@emoryhealthcare.org; TanyaAbbott@catholichealth.net; histonet@lists.utsouthwestern.edu Date: Thu, 9 Oct 2014 19:52:12 +0000 CC: Subject: [Histonet] RE: Ebola Have it delivered in formalin and let it fix completely. "Formalin fixation eliminates the biologic hazard associated with transporting samples containing infectious EBO virus, .................... Thus, formalin fixation provides an adequate noninfectious specimen for EHF diagnosis, ........................." A quote from: Long-Term Disease Surveillance in Bandundu Region, Democratic Republic of the Congo: A Model for Early Detection and Prevention of Ebola Hemorrhagic Fever Ethleen S. Lloyd, and C. J. Peters, et al + Author Affiliations Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization and US Agency for International Development and Ministry of Health, Kinshasa, H?pital G?n?ral de R?f?rence de Kikwit, Kikwit, and Mosango Mission Hospital, Mosango, Democratic Republic of the Congo; M?decins sans Fronti?res?Belgium, Brussels, Belgium + Author Notes ?* Current affiliation: Institut de M?decine Tropicale, Antwerp, Belgium (M.A.B.); M?decins sans Fronti?res?Belgium, N'Djamena, Chad (E.V.); World Health Organization, Kinshasa, Democratic Republic of the Congo (J.K.). Reprints or correspondence: Dr. Pierre E. Rollin, Special Pathogens Branch, NCID/DVRD, Mailstop G-14, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333 (pyr3@cdc.gov). James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, October 09, 2014 12:22 PM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ebola Handle with universal precautions as always is our emphasis. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Thursday, October 09, 2014 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ebola Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Thu Oct 9 15:34:28 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Thu Oct 9 15:34:31 2014 Subject: [Histonet] Ebola In-Reply-To: <1331788667.1679788.1412886376853.JavaMail.root@comcast.net> References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net>, <1331788667.1679788.1412886376853.JavaMail.root@comcast.net> Message-ID: Well said Pam, it is just assumed that formalin will eliminate the biohaz for Ebola, I doubt if that has been conclusively proven yet, remember we only discovered fairly recently that formalin fixation did not protect us from CJD!!!! Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net > Date: Thu, 9 Oct 2014 20:26:16 +0000 > From: mucram11@comcast.net > To: TanyaAbbott@catholichealth.net > Subject: Re: [Histonet] Ebola > CC: histonet@lists.utsouthwestern.edu > > Take Brett's advise and use that as guidleine. We don't know as much as we should about these viruses. Pam > > ----- Original Message ----- > > From: "Tanya Abbott" > To: "Histonet" > Sent: Thursday, October 9, 2014 2:03:47 PM > Subject: [Histonet] Ebola > > Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology > St. Joseph Medical Center > Reading, PA 19603-0316 > ph 610-378-2635 > fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Oct 9 15:40:20 2014 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Thu Oct 9 15:40:25 2014 Subject: [Histonet] Ebola In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> <1331788667.1679788.1412886376853.JavaMail.root@comcast.net> Message-ID: If formalin didn't kill CJD, what did you use? Just curious. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg wrote: > Well said Pam, it is just assumed that formalin will eliminate the biohaz > for Ebola, I doubt if that has been conclusively proven yet, remember we > only discovered fairly recently that formalin fixation did not protect us > from CJD!!!! > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > pruegg@ihctech.net > > > > Date: Thu, 9 Oct 2014 20:26:16 +0000 > > From: mucram11@comcast.net > > To: TanyaAbbott@catholichealth.net > > Subject: Re: [Histonet] Ebola > > CC: histonet@lists.utsouthwestern.edu > > > > Take Brett's advise and use that as guidleine. We don't know as much as > we should about these viruses. Pam > > > > ----- Original Message ----- > > > > From: "Tanya Abbott" > > To: "Histonet" > > Sent: Thursday, October 9, 2014 2:03:47 PM > > Subject: [Histonet] Ebola > > > > Dare I ask?! Are any Pathology labs discussing what to do with > specimens/precautions, etc. regarding a person with a potential Ebola > infection? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology > > St. Joseph Medical Center > > Reading, PA 19603-0316 > > ph 610-378-2635 > > fax 610-898-5871 > > email: tanyaabbott@catholichealth.net > > > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are not > an addressee, or responsible for delivering this email to an addressee, you > have received this email in error and are notified that reading, copying, > or disclosing this email is prohibited. If you received this email in > error, immediately reply to the sender and delete the message completely > from your computer system. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWatson <@t> gnf.org Thu Oct 9 15:56:36 2014 From: JWatson <@t> gnf.org (James Watson) Date: Thu Oct 9 15:56:41 2014 Subject: [Histonet] Ebola In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> <1331788667.1679788.1412886376853.JavaMail.root@comcast.net> Message-ID: Back in the 80 NIH did extensive studies on CJD, their protocol requires 24 hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, then paraffin processing. Worked with CJD at Frederick Cancer Research in collaboration with the group from NIH. If I find the publications I will forward them. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, October 09, 2014 1:40 PM Cc: Histonet@Lists. Edu Subject: Re: [Histonet] Ebola If formalin didn't kill CJD, what did you use? Just curious. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg wrote: > Well said Pam, it is just assumed that formalin will eliminate the > biohaz for Ebola, I doubt if that has been conclusively proven yet, > remember we only discovered fairly recently that formalin fixation did > not protect us from CJD!!!! > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > pruegg@ihctech.net > > > > Date: Thu, 9 Oct 2014 20:26:16 +0000 > > From: mucram11@comcast.net > > To: TanyaAbbott@catholichealth.net > > Subject: Re: [Histonet] Ebola > > CC: histonet@lists.utsouthwestern.edu > > > > Take Brett's advise and use that as guidleine. We don't know as > > much as > we should about these viruses. Pam > > > > ----- Original Message ----- > > > > From: "Tanya Abbott" > > To: "Histonet" > > Sent: Thursday, October 9, 2014 2:03:47 PM > > Subject: [Histonet] Ebola > > > > Dare I ask?! Are any Pathology labs discussing what to do with > specimens/precautions, etc. regarding a person with a potential Ebola > infection? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology St. Joseph Medical Center > > Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > > email: tanyaabbott@catholichealth.net > > > > This electronic mail and any attached documents are intended solely > > for > the named addressee(s) and contain confidential information. If you > are not an addressee, or responsible for delivering this email to an > addressee, you have received this email in error and are notified that > reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and > delete the message completely from your computer system. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Oct 9 16:09:20 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Oct 9 16:15:28 2014 Subject: [Histonet] Ebola In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net>, <1331788667.1679788.1412886376853.JavaMail.root@comcast.net> Message-ID: Actually it has been proven that formalin will inactivate Ebola, as well as other hemorrhagic fever viruses. Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, October 09, 2014 4:34 PM To: Pam Marcum; Tanya Abbott Cc: Histonet@Lists. Edu Subject: RE: [Histonet] Ebola Well said Pam, it is just assumed that formalin will eliminate the biohaz for Ebola, I doubt if that has been conclusively proven yet, remember we only discovered fairly recently that formalin fixation did not protect us from CJD!!!! Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net > Date: Thu, 9 Oct 2014 20:26:16 +0000 > From: mucram11@comcast.net > To: TanyaAbbott@catholichealth.net > Subject: Re: [Histonet] Ebola > CC: histonet@lists.utsouthwestern.edu > > Take Brett's advise and use that as guidleine. We don't know as much as we should about these viruses. Pam > > ----- Original Message ----- > > From: "Tanya Abbott" > To: "Histonet" > Sent: Thursday, October 9, 2014 2:03:47 PM > Subject: [Histonet] Ebola > > Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection? > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology > St. Joseph Medical Center > Reading, PA 19603-0316 > ph 610-378-2635 > fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From JWatson <@t> gnf.org Thu Oct 9 16:31:38 2014 From: JWatson <@t> gnf.org (James Watson) Date: Thu Oct 9 16:31:42 2014 Subject: [Histonet] Ebola In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> <1331788667.1679788.1412886376853.JavaMail.root@comcast.net> Message-ID: Correction, due to years of formalin abuse, it was 1 hour in concentrated formic acid. We then processed and cut them with all other cases. A few cases slipped through with just formalin fixation and additional precautions needed to be taken. That said the easiest and primary route of infection for CJD is ingestion. Needless to say we did not eat any of the tissue. A simple and effective method for inactivating virus infectivity in formalin?fixed tissue samples from patients with Creutzfeldt?Jakob disease Paul Brown, MD, Axel Wolff, DVM and D. Carleton Gajdusek, MD -Show Affiliations Laboratory of CNS Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD. doi: 10.1212/WNL.40.6.887 Neurology June 1990 vol. 40 no. 6 887 Abstract Full Text (PDF) Abstract We fixed brains from hamsters infected with scrapie virus in (1) formalin, (2) phenol-saturated formalin, (3) formalin with a 1-hour immersion in formic acid, or (4) phenol-saturated formalin with a 1-hour immersion in formic acid. In addition, we used the formalin-formic acid procedure on brains from mice infected with the virus of Creutzfeldt-Jakob disease. Formic acid proved superior to phenol in respect to both disinfection and tissue preservation, almost completely eliminating virus infectivity in sections that were histologically indistinguishable from formalin-fixed material. The inclusion of a formic acid step in routine formaldehyde tissue fixation will thus provide histologic sections of excellent quality, and virtually eliminate the risk of handling infectious material in the subsequent neuropathologic processing of tissues from patients with CJD. ? 1990 by the American Academy of Neurology James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, October 09, 2014 1:57 PM To: 'Emily Brown' Cc: Histonet@Lists. Edu Subject: RE: [Histonet] Ebola Back in the 80 NIH did extensive studies on CJD, their protocol requires 24 hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, then paraffin processing. Worked with CJD at Frederick Cancer Research in collaboration with the group from NIH. If I find the publications I will forward them. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, October 09, 2014 1:40 PM Cc: Histonet@Lists. Edu Subject: Re: [Histonet] Ebola If formalin didn't kill CJD, what did you use? Just curious. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg wrote: > Well said Pam, it is just assumed that formalin will eliminate the > biohaz for Ebola, I doubt if that has been conclusively proven yet, > remember we only discovered fairly recently that formalin fixation did > not protect us from CJD!!!! > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > pruegg@ihctech.net > > > > Date: Thu, 9 Oct 2014 20:26:16 +0000 > > From: mucram11@comcast.net > > To: TanyaAbbott@catholichealth.net > > Subject: Re: [Histonet] Ebola > > CC: histonet@lists.utsouthwestern.edu > > > > Take Brett's advise and use that as guidleine. We don't know as > > much as > we should about these viruses. Pam > > > > ----- Original Message ----- > > > > From: "Tanya Abbott" > > To: "Histonet" > > Sent: Thursday, October 9, 2014 2:03:47 PM > > Subject: [Histonet] Ebola > > > > Dare I ask?! Are any Pathology labs discussing what to do with > specimens/precautions, etc. regarding a person with a potential Ebola > infection? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology St. Joseph Medical Center > > Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > > email: tanyaabbott@catholichealth.net > > > > This electronic mail and any attached documents are intended solely > > for > the named addressee(s) and contain confidential information. If you > are not an addressee, or responsible for delivering this email to an > addressee, you have received this email in error and are notified that > reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and > delete the message completely from your computer system. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Thu Oct 9 16:56:41 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Thu Oct 9 16:56:47 2014 Subject: [Histonet] Ebola In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net>, <1331788667.1679788.1412886376853.JavaMail.root@comcast.net>, , , , Message-ID: We tried very hard not to do CJD cases if we could help it, as I recall any suspect cases were sent to Case Western Reserve, i worked with someone there who developed a protocol using strong formic acid, it was amazing how good the tissue preservation was using formic acid as a fixative. I heard about possible cases in our histo dept at the u where they ended up throwing away microtomes and other instruments who might have come in contact with CJD tissue, crazy, wasn't my department so I was not up on all the details. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From: JWatson@gnf.org To: histonet@lists.utsouthwestern.edu; SFinley@providencehealth.bc.ca Date: Thu, 9 Oct 2014 21:31:38 +0000 Subject: RE: [Histonet] Ebola CC: Correction, due to years of formalin abuse, it was 1 hour in concentrated formic acid. We then processed and cut them with all other cases. A few cases slipped through with just formalin fixation and additional precautions needed to be taken. That said the easiest and primary route of infection for CJD is ingestion. Needless to say we did not eat any of the tissue. A simple and effective method for inactivating virus infectivity in formalin?fixed tissue samples from patients with Creutzfeldt?Jakob disease Paul Brown, MD, Axel Wolff, DVM and D. Carleton Gajdusek, MD -Show Affiliations Laboratory of CNS Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD. doi: 10.1212/WNL.40.6.887 Neurology June 1990 vol. 40 no. 6 887 Abstract Full Text (PDF) Abstract We fixed brains from hamsters infected with scrapie virus in (1) formalin, (2) phenol-saturated formalin, (3) formalin with a 1-hour immersion in formic acid, or (4) phenol-saturated formalin with a 1-hour immersion in formic acid. In addition, we used the formalin-formic acid procedure on brains from mice infected with the virus of Creutzfeldt-Jakob disease. Formic acid proved superior to phenol in respect to both disinfection and tissue preservation, almost completely eliminating virus infectivity in sections that were histologically indistinguishable from formalin-fixed material. The inclusion of a formic acid step in routine formaldehyde tissue fixation will thus provide histologic sections of excellent quality, and virtually eliminate the risk of handling infectious material in the subsequent neuropathologic processing of tissues from patients with CJD. ? 1990 by the American Academy of Neurology James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, October 09, 2014 1:57 PM To: 'Emily Brown' Cc: Histonet@Lists. Edu Subject: RE: [Histonet] Ebola Back in the 80 NIH did extensive studies on CJD, their protocol requires 24 hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, then paraffin processing. Worked with CJD at Frederick Cancer Research in collaboration with the group from NIH. If I find the publications I will forward them. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, October 09, 2014 1:40 PM Cc: Histonet@Lists. Edu Subject: Re: [Histonet] Ebola If formalin didn't kill CJD, what did you use? Just curious. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg wrote: > Well said Pam, it is just assumed that formalin will eliminate the > biohaz for Ebola, I doubt if that has been conclusively proven yet, > remember we only discovered fairly recently that formalin fixation did > not protect us from CJD!!!! > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > pruegg@ihctech.net > > > > Date: Thu, 9 Oct 2014 20:26:16 +0000 > > From: mucram11@comcast.net > > To: TanyaAbbott@catholichealth.net > > Subject: Re: [Histonet] Ebola > > CC: histonet@lists.utsouthwestern.edu > > > > Take Brett's advise and use that as guidleine. We don't know as > > much as > we should about these viruses. Pam > > > > ----- Original Message ----- > > > > From: "Tanya Abbott" > > To: "Histonet" > > Sent: Thursday, October 9, 2014 2:03:47 PM > > Subject: [Histonet] Ebola > > > > Dare I ask?! Are any Pathology labs discussing what to do with > specimens/precautions, etc. regarding a person with a potential Ebola > infection? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology St. Joseph Medical Center > > Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > > email: tanyaabbott@catholichealth.net > > > > This electronic mail and any attached documents are intended solely > > for > the named addressee(s) and contain confidential information. If you > are not an addressee, or responsible for delivering this email to an > addressee, you have received this email in error and are notified that > reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and > delete the message completely from your computer system. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Fri Oct 10 08:43:06 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Oct 10 08:43:13 2014 Subject: [Histonet] RE: Ebola In-Reply-To: <20141009210626.A00FD1E897F@trendmess-svr.holyredeemer.local> References: <20141009210626.A00FD1E897F@trendmess-svr.holyredeemer.local> Message-ID: My area has a large west African population and we have already established a interim procedure based on the CDC's guidelines, and the Philadelphia Dept of Health, Division of Disease control. We also have a response committee that is actively reviewing this procedure to insure that it is up to date and that all involved are educated on how to handle their piece. It includes: response for any patient presenting with fever, patient interviews including travel history, assessment of exposure risk, reporting, isolation and housing precautions for suspect patients, transportation and handling of specimens, and heaven forbid, morgue procedures. Universal precautions are just not enough. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 ************* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Joyce.Weems <@t> emoryhealthcare.org Fri Oct 10 08:49:33 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Oct 10 08:49:48 2014 Subject: [Histonet] RE: Ebola In-Reply-To: References: <20141009210626.A00FD1E897F@trendmess-svr.holyredeemer.local> Message-ID: We have the same - a task force with evolving info - with early input from the CDC, but universal precautions are emphasized even more now. Emory is really on top of it all!! My best to everyone and happy Friday!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Friday, October 10, 2014 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ebola My area has a large west African population and we have already established a interim procedure based on the CDC's guidelines, and the Philadelphia Dept of Health, Division of Disease control. We also have a response committee that is actively reviewing this procedure to insure that it is up to date and that all involved are educated on how to handle their piece. It includes: response for any patient presenting with fever, patient interviews including travel history, assessment of exposure risk, reporting, isolation and housing precautions for suspect patients, transportation and handling of specimens, and heaven forbid, morgue procedures. Universal precautions are just not enough. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 ************* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From brett_connolly <@t> merck.com Fri Oct 10 08:52:36 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Oct 10 08:52:42 2014 Subject: [Histonet] RE: Ebola In-Reply-To: References: <20141009210626.A00FD1E897F@trendmess-svr.holyredeemer.local> Message-ID: Here is the CDC guidelines link for those interested http://www.cdc.gov/vhf/ebola/ Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Friday, October 10, 2014 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ebola My area has a large west African population and we have already established a interim procedure based on the CDC's guidelines, and the Philadelphia Dept of Health, Division of Disease control. We also have a response committee that is actively reviewing this procedure to insure that it is up to date and that all involved are educated on how to handle their piece. It includes: response for any patient presenting with fever, patient interviews including travel history, assessment of exposure risk, reporting, isolation and housing precautions for suspect patients, transportation and handling of specimens, and heaven forbid, morgue procedures. Universal precautions are just not enough. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 ************* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Vickroy.Jim <@t> mhsil.com Fri Oct 10 09:27:34 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Oct 10 09:27:41 2014 Subject: [Histonet] New Common Checklist for Anatomic Pathology Message-ID: Before I ask CAP I thought I would see if anyone has had a CAP inspection using the new checklists from 4-21-2014. I am especially puzzled about a lot of the questions in the common checklist. Although I clearly understand that some apply to anatomic pathology as well as all parts of the lab, there are a lot of questions that just don't seem to apply to surgical pathology and histology. Can anyone give me any idea of how the common checklist was used when they were inspected recently? Were there a lot of questions that were just not applicable? PS. I realize your experiences are not how each inspector interprets each question but just trying to get a general feeling. I am not a novice to inspections since I have 35 years in Histology and Anatomic Pathology, but I am trying to make sure I keep up with changes and also don't read more into things than is necessary. Jim ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 10 10:29:31 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Oct 10 10:29:36 2014 Subject: [Histonet] Histotechnologist: What my friends think I do Message-ID: <761E2B5697F795489C8710BCC72141FF3679D8F1@ex07.net.ucsf.edu> Try this one again, for TGIF. http://histosearch.com/imageupload/what-my-friends-think-i-do-histotech/ Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. From mpence <@t> grhs.net Fri Oct 10 10:33:34 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Oct 10 10:33:56 2014 Subject: [Histonet] RE: Histotechnologist: What my friends think I do In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679D8F1@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF3679D8F1@ex07.net.ucsf.edu> Message-ID: Nice! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, October 10, 2014 10:31 AM To: Histonet Subject: [Histonet] Histotechnologist: What my friends think I do Try this one again, for TGIF. http://histosearch.com/imageupload/what-my-friends-think-i-do-histotech/ Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah.Dysart <@t> stdavids.com Fri Oct 10 10:49:43 2014 From: Sarah.Dysart <@t> stdavids.com (Sarah.Dysart@stdavids.com) Date: Fri Oct 10 10:49:48 2014 Subject: [Histonet] Another TGIF Friday... Message-ID: <34C22BB94729434598D767D3F4EB95E0F48E109CFD@FWDCWPMSGCMS03.hca.corpad.net> Does anyone have that thing handy that says what different positions in the histology lab do? It's like Superman, leaping buildings and such... Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 From brett_connolly <@t> merck.com Fri Oct 10 11:10:08 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Oct 10 11:10:57 2014 Subject: [Histonet] More TGIF Message-ID: Enjoy some histotechnologist logos...... http://www.zazzle.com/histotechnologist Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Joyce.Weems <@t> emoryhealthcare.org Fri Oct 10 11:35:12 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Oct 10 11:35:20 2014 Subject: [Histonet] RE: Another TGIF Friday... In-Reply-To: <34C22BB94729434598D767D3F4EB95E0F48E109CFD@FWDCWPMSGCMS03.hca.corpad.net> References: <34C22BB94729434598D767D3F4EB95E0F48E109CFD@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: "LAB HIERARCHY" Chief Pathologist Leaps tall building in a single bound Is more powerful than a locomotive Is faster than a speeding bullet Walks on water, Gives policy to God Associate Pathologist Leaps short buildings in single bound Is more powerful than a switch engine Is just as fast as a speeding bullet Walks on water if sea is calm Talks with God The Department Head Leaps short buildings with a running start and favorable winds Is almost as powerful as a switch engine Is faster than a speeding bullet Walks on water in an indoor pool Talks with God if special request is approved Director of Laboratories Rarely clears a Quonset hut Loses tug of war with locomotive Can fire a speeding bullet Swims well Is occasionally addressed by God Associate Director of Laboratories Makes high marks on the walls when trying to leap tall buildings Is run over by locomotives Can sometimes handle a gun without inflicting self-injury Dog paddles Talks to animals Supervisor Runs into building Recognizes locomotives two out of three times Is not issued ammunition Can stay afloat with a life jacket Talks to walls Chief Technologist Falls over doorstep when trying to enter building Says "look at the choo-choo" Wets themselves with a water pistol Plays in mud puddles Mumbles to Themselves Histotechnologist Lifts tall buildings and walks under them Kicks locomotives off the tracks Catches speeding bullets in their teeth and eats them Freezes water with a single glance They are God Anonymous Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com Sent: Friday, October 10, 2014 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Another TGIF Friday... Does anyone have that thing handy that says what different positions in the histology lab do? It's like Superman, leaping buildings and such... Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Joyce.Weems <@t> emoryhealthcare.org Fri Oct 10 11:41:13 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Oct 10 11:41:20 2014 Subject: [Histonet] RE: Ebola In-Reply-To: References: <20141009210626.A00FD1E897F@trendmess-svr.holyredeemer.local> Message-ID: I have an article that will be coming out in the next Laboratory Medicine - done by Emory early on - describes the POC lab used for our two patients. If anyone would like it let me know.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, October 10, 2014 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ebola Here is the CDC guidelines link for those interested http://www.cdc.gov/vhf/ebola/ Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Friday, October 10, 2014 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ebola My area has a large west African population and we have already established a interim procedure based on the CDC's guidelines, and the Philadelphia Dept of Health, Division of Disease control. We also have a response committee that is actively reviewing this procedure to insure that it is up to date and that all involved are educated on how to handle their piece. It includes: response for any patient presenting with fever, patient interviews including travel history, assessment of exposure risk, reporting, isolation and housing precautions for suspect patients, transportation and handling of specimens, and heaven forbid, morgue procedures. Universal precautions are just not enough. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 ************* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From mjones <@t> metropath.com Fri Oct 10 12:13:06 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Fri Oct 10 12:13:13 2014 Subject: [Histonet] RE: Another TGIF Friday... Message-ID: Oh boy! Now that?s funny!! Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 10/10/14, 10:35 AM, "Weems, Joyce K." wrote: >"LAB HIERARCHY" > >Chief Pathologist > Leaps tall building in a single bound > Is more powerful than a locomotive > Is faster than a speeding bullet > Walks on water, > Gives policy to God > >Associate Pathologist > Leaps short buildings in single bound > Is more powerful than a switch engine > Is just as fast as a speeding bullet > Walks on water if sea is calm > Talks with God > >The Department Head > Leaps short buildings with a running start and favorable winds > Is almost as powerful as a switch engine > Is faster than a speeding bullet > Walks on water in an indoor pool > Talks with God if special request is approved > >Director of Laboratories > Rarely clears a Quonset hut > Loses tug of war with locomotive > Can fire a speeding bullet > Swims well > Is occasionally addressed by God > >Associate Director of Laboratories > Makes high marks on the walls when trying to leap tall buildings > Is run over by locomotives > Can sometimes handle a gun without inflicting self-injury > Dog paddles > Talks to animals > >Supervisor > Runs into building > Recognizes locomotives two out of three times > Is not issued ammunition > Can stay afloat with a life jacket > Talks to walls > >Chief Technologist > Falls over doorstep when trying to enter building > Says "look at the choo-choo" > Wets themselves with a water pistol > Plays in mud puddles > Mumbles to Themselves > >Histotechnologist > Lifts tall buildings and walks under them > Kicks locomotives off the tracks > Catches speeding bullets in their teeth and eats them > Freezes water with a single glance > They are God > >Anonymous > >Joyce Weems >Pathology Manager >678-843-7376 Phone >678-843-7831 Fax >joyce.weems@emoryhealthcare.org > > > >www.saintjosephsatlanta.org >5665 Peachtree Dunwoody Road >Atlanta, GA 30342 > >This e-mail, including any attachments is the property of Saint Joseph's >Hospital and is intended for the sole use of the intended recipient(s). >It may contain information that is privileged and confidential. Any >unauthorized review, use, disclosure, or distribution is prohibited. If >you are not the intended recipient, please delete this message, and reply >to the sender regarding the error in a separate email. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Sarah.Dysart@stdavids.com >Sent: Friday, October 10, 2014 11:50 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Another TGIF Friday... > >Does anyone have that thing handy that says what different positions in >the histology lab do? It's like Superman, leaping buildings and such... > >Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. >David's North Austin Medical Center >12221 North Mopac Expressway >Austin, Texas 78758 >(512)901-1220 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >________________________________ > >This e-mail message (including any attachments) is for the sole use of >the intended recipient(s) and may contain confidential and privileged >information. If the reader of this message is not the intended >recipient, you are hereby notified that any dissemination, distribution >or copying of this message (including any attachments) is strictly >prohibited. > >If you have received this message in error, please contact >the sender by reply e-mail message and destroy all copies of the >original message (including attachments). > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shawn.Griffin <@t> tmfhc.org Fri Oct 10 12:36:09 2014 From: Shawn.Griffin <@t> tmfhc.org (Shawn Griffin) Date: Fri Oct 10 12:36:17 2014 Subject: [Histonet] Ventana Benchmark Ultra Stainer Validation Message-ID: <2aac11672c3b41e6a3e4a5a46ac1613e@BLUPR01MB405.prod.exchangelabs.com> I am looking into upgrading my Ventana Benchmark Ultras to the Ultras that have the Ultimate Reagent Access. Can anyone tell me what the benefits have been for them? What did you do to revalidate your instrument? Thanks in advance for the input. Shawn H Griffin, HT ASCP(QIHC) AP Supervisor Trinity Mother Frances Hospital and Clinics 800 East Dawson Tyler, Texas 75701 P 903-525-2728 F 903-531-4675 E-mail: shawn.griffin@tmfhc.org This email message and attachments may contain confidential and privileged information that is protected by law. This email is transmitted for the sole use of the intended recipient. If you are not the intended recipient, you have received this message in error and use of this information is prohibited. Please delete this message and destroy any copies at once and notify the sender by email or phone of the error. From abadesuyi <@t> nrh-ok.com Fri Oct 10 12:38:55 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Fri Oct 10 12:38:59 2014 Subject: [Histonet] BMSS Message-ID: <04EE4F75BB5FB246ADB68D69B74604439468DA8843@MAIL.nrhnt.nrh-ok.com> Hi, I hope that you guys are doing great. Please I have a question for you guys that use the new Ventana Bench Mark Special stainer (i.e. BKSS). Since we bought the stainer, we couldn't get the PASD to work. I will like to hear from people out there that are using this stainer and did not have any problem with the PASD Stain. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor, Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From ilyagitlin <@t> gmail.com Fri Oct 10 12:42:23 2014 From: ilyagitlin <@t> gmail.com (Ilya Gitlin) Date: Fri Oct 10 12:42:48 2014 Subject: [Histonet] Looking for a pathologist in Houston Message-ID: Hello Histonetters, We are opening a new diagnostic laboratory in Houston, TX. We are currently looking for a licensed pathologist if interested please contact Greg Gitlin at: info@lodestardiagnostic.com Greg Gitlin Vice President of Operation Lodestar Diagnostic Laboratory 11301 Fallbrook Dr. Suite 108 Houston, TX 77065 P: (832) 912 4494 <%28832%29%20912%204494> F: (832) 912 4059 <%28832%29%20912%204059> From Joyce.Weems <@t> emoryhealthcare.org Fri Oct 10 14:13:19 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Oct 10 14:13:30 2014 Subject: [Histonet] Ebola In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF394E70D4@CHIEX005.CHI.catholichealth.net> <1331788667.1679788.1412886376853.JavaMail.root@comcast.net> Message-ID: http://news.discovery.com/human/health/why-ebola-wont-spread-in-the-united-states-141009.htm http://www.voanews.com/media/video/us-doctors-learn-from-treating-ebola-patients/2477893.html http://www.washingtonpost.com/lifestyle/travel/tips-for-traveling-to-africa-in-the-age-of-ebola/2014/10/09/46af8ae8-4efd-11e4-8c24-487e92bc997b_story.html In our Emory news today... I haven't listened yet, but you all might like to look... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, October 09, 2014 5:32 PM To: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu); 'Finley, Sue [PH]' Subject: RE: [Histonet] Ebola Correction, due to years of formalin abuse, it was 1 hour in concentrated formic acid. We then processed and cut them with all other cases. A few cases slipped through with just formalin fixation and additional precautions needed to be taken. That said the easiest and primary route of infection for CJD is ingestion. Needless to say we did not eat any of the tissue. A simple and effective method for inactivating virus infectivity in formalin?fixed tissue samples from patients with Creutzfeldt?Jakob disease Paul Brown, MD, Axel Wolff, DVM and D. Carleton Gajdusek, MD -Show Affiliations Laboratory of CNS Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD. doi: 10.1212/WNL.40.6.887 Neurology June 1990 vol. 40 no. 6 887 Abstract Full Text (PDF) Abstract We fixed brains from hamsters infected with scrapie virus in (1) formalin, (2) phenol-saturated formalin, (3) formalin with a 1-hour immersion in formic acid, or (4) phenol-saturated formalin with a 1-hour immersion in formic acid. In addition, we used the formalin-formic acid procedure on brains from mice infected with the virus of Creutzfeldt-Jakob disease. Formic acid proved superior to phenol in respect to both disinfection and tissue preservation, almost completely eliminating virus infectivity in sections that were histologically indistinguishable from formalin-fixed material. The inclusion of a formic acid step in routine formaldehyde tissue fixation will thus provide histologic sections of excellent quality, and virtually eliminate the risk of handling infectious material in the subsequent neuropathologic processing of tissues from patients with CJD. ? 1990 by the American Academy of Neurology James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, October 09, 2014 1:57 PM To: 'Emily Brown' Cc: Histonet@Lists. Edu Subject: RE: [Histonet] Ebola Back in the 80 NIH did extensive studies on CJD, their protocol requires 24 hours in formalin, 24 hours in formic acid, followed by 48 hours in formalin, then paraffin processing. Worked with CJD at Frederick Cancer Research in collaboration with the group from NIH. If I find the publications I will forward them. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, October 09, 2014 1:40 PM Cc: Histonet@Lists. Edu Subject: Re: [Histonet] Ebola If formalin didn't kill CJD, what did you use? Just curious. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Thu, Oct 9, 2014 at 4:34 PM, Patsy Ruegg wrote: > Well said Pam, it is just assumed that formalin will eliminate the > biohaz for Ebola, I doubt if that has been conclusively proven yet, > remember we only discovered fairly recently that formalin fixation did > not protect us from CJD!!!! > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > pruegg@ihctech.net > > > > Date: Thu, 9 Oct 2014 20:26:16 +0000 > > From: mucram11@comcast.net > > To: TanyaAbbott@catholichealth.net > > Subject: Re: [Histonet] Ebola > > CC: histonet@lists.utsouthwestern.edu > > > > Take Brett's advise and use that as guidleine. We don't know as > > much as > we should about these viruses. Pam > > > > ----- Original Message ----- > > > > From: "Tanya Abbott" > > To: "Histonet" > > Sent: Thursday, October 9, 2014 2:03:47 PM > > Subject: [Histonet] Ebola > > > > Dare I ask?! Are any Pathology labs discussing what to do with > specimens/precautions, etc. regarding a person with a potential Ebola > infection? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology St. Joseph Medical Center > > Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > > email: tanyaabbott@catholichealth.net > > > > This electronic mail and any attached documents are intended solely > > for > the named addressee(s) and contain confidential information. If you > are not an addressee, or responsible for delivering this email to an > addressee, you have received this email in error and are notified that > reading, copying, or disclosing this email is prohibited. If you > received this email in error, immediately reply to the sender and > delete the message completely from your computer system. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From tgenade <@t> gmail.com Fri Oct 10 14:44:20 2014 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Oct 10 14:44:26 2014 Subject: [Histonet] can you defrost and fix muscle tissue? Message-ID: Hello, A fellow faculty member had some rat muscle tissue that was set in tissue freezing medium for frozen sectioning. Some sections were cut (just as well as you may realize as you read on) and then the decisions was made to PFA fix and embed in wax. I didn't like the idea as it meant thawing the tissue and possibly introducing freeze-thaw artifacts and compromise the tissue... But the decision was decided to proceed. The samples were incubated in 4% PFA for 24 hours. The pieces of tissue were about 1.5 to 2 cm (cubic). I took issue with the small volumes of 4% PFA were used (a student was set to do the task) and the volume was greatly increased and incubated for another 24 hours... We then set about dehydrating, clearing and embedding in wax. Today, with the student, I tried cutting a few ribbons but it was utterly hopeless. The sections kept crumbling as I cut (thin or thick sections). Is the issue: a) fixing frozen tissue b) improper fixation c) something else? To exclude a problem with the wax, some sections of the wax (no tissue) were cut and they were perfect. Regretfully, no fresh muscle was fixed for comparison but that is going to happen if I am asked to repeat this. For muscle tissue, would you in future suggest PFA or Bouin's? The person is interested in analyzing blood vessels. The rats are hypertensive so I'm thinking Bouin's in case a follow-up study, staining for collagen (scare tissue, artheroclerosis) is desired. I would like to provide a good explanation for why the sections are crumbling... Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From liz <@t> premierlab.com Fri Oct 10 15:12:03 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Oct 10 15:12:08 2014 Subject: [Histonet] can you defrost and fix muscle tissue? In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD9A7@SBS2K8.premierlab.local> Tyrone As long as the tissue has been frozen properly you should not have an issue with taking a frozen block to paraffin. If the tissue has been frozen improperly (slow freezing) then you may encounter some freeze artifact. We have done it on several occasions without any adverse effects. We place the frozen sample directly into the fixative rather than thawing and then fixing, replace the fixative and then process as usual. Sounds more like a processing problem to me, possibly over processed? Once the blocks were trimmed did you place on wet ice prior to sectioning? For fixation I would use 4% PFA or 10% NBF. For staining I would use a modified trichrome one that combines elastic and trichrome staining since you may get disruption of the elastic fibers in atherosclerosis. We did not work in rat models but we looked at rabbit and mouse models for atherosclerosis, we stained IHC for macrophages, smooth muscle actin and CD31. Good Luck. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Friday, October 10, 2014 1:44 PM To: histonet Subject: [Histonet] can you defrost and fix muscle tissue? Hello, A fellow faculty member had some rat muscle tissue that was set in tissue freezing medium for frozen sectioning. Some sections were cut (just as well as you may realize as you read on) and then the decisions was made to PFA fix and embed in wax. I didn't like the idea as it meant thawing the tissue and possibly introducing freeze-thaw artifacts and compromise the tissue... But the decision was decided to proceed. The samples were incubated in 4% PFA for 24 hours. The pieces of tissue were about 1.5 to 2 cm (cubic). I took issue with the small volumes of 4% PFA were used (a student was set to do the task) and the volume was greatly increased and incubated for another 24 hours... We then set about dehydrating, clearing and embedding in wax. Today, with the student, I tried cutting a few ribbons but it was utterly hopeless. The sections kept crumbling as I cut (thin or thick sections). Is the issue: a) fixing frozen tissue b) improper fixation c) something else? To exclude a problem with the wax, some sections of the wax (no tissue) were cut and they were perfect. Regretfully, no fresh muscle was fixed for comparison but that is going to happen if I am asked to repeat this. For muscle tissue, would you in future suggest PFA or Bouin's? The person is interested in analyzing blood vessels. The rats are hypertensive so I'm thinking Bouin's in case a follow-up study, staining for collagen (scare tissue, artheroclerosis) is desired. I would like to provide a good explanation for why the sections are crumbling... Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Oct 11 13:35:24 2014 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Oct 11 13:35:31 2014 Subject: [Histonet] Re: can you defrost and fix muscle tissue? Message-ID: <1413052520675.1361@kcl.ac.uk> Your project reads ill-thought out! I agree with Elizabeth re defrosting. Should be fine. Yes, if your freezing is slow, you will most likely have ice-crystal artefact.? You will see this in the Pwax sections. You have large pieces of muscle. Good that you fixed for longer. Imho, if you are not requiring Immunostaining, the longer the fixation...the better ( within reason) I would assume your problems are a result of UNDER processing. Muscle pieces that size, I would always process to Pwax over 40hr schedule. What was your processing schedule? I often Pwax process 1x1x0.5cm pig skeletal muscle and have no problems cutting....nope, no ice involved. Just a sharp knife and, a gentle rub of the block face every section, using 20% alcohol -damped soft tissue. If you are examining blood vessels....you HAVE to perfuse-fix, imho.....for best results. When you get damn fine sections: Do an H&E and an HVG, initially. As Elizabeth stated....stick to Formalin fixation. NB: PFA is not a fixative. It is a polymer of methylene glycol and is...a powder! When dissolved it forms multiple forms : methylene glycol is the monomer. What you buy when you get "40% Formalin" is methylene glycol in solution ( Formaldehyde gas dissolves in water to ~ 40% saturation). Dilute that 1/10 and you get 10% Formalin ( same as 4% "PFA") The ONLY reason them Molecular biologists/chemists decided to use PFA is cos they have NO idea re Formalin/methylene glycol fixation! Neither do I, chuckle. Well.....the reason they decided to use Paraformaldehyde powder is because the immediate product is , more or less, pure methylene glycol. In bought 40% Formalin, they add a small amount of alcohol to retard formation of Formic acid and polymerised methylene glycol( PFA). So.....them geezers thought: OMG...alcohol! No....it will change the chemistry of fixation! Sure....them geezers NEVER bothered to look at most clinical labs in the world who buy/use ~40% Formalin to make up their 10% Formalin....with great success!! Imho, nobody has produced a convincing raison d'?tre for adding PBS. It is added because we think of physiology....add PBS to a fix and we get ..."better" fixation. Chuckle....Formalin ( methylene glycol) KILLS cells. Adding PBS...hmmm. I still do.....chuckle. Curiously, as ever. carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From LSebree <@t> uwhealth.org Sat Oct 11 16:55:24 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Sat Oct 11 16:55:31 2014 Subject: [Histonet] RE: Another TGIF Friday... In-Reply-To: References: <34C22BB94729434598D767D3F4EB95E0F48E109CFD@FWDCWPMSGCMS03.hca.corpad.net>, Message-ID: <77DD817201982748BC67D7960F2F76AF0E1F76@UWHC-MBX12.uwhis.hosp.wisc.edu> This is by far my favorite! Linda A. Sebree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Weems, Joyce K. [Joyce.Weems@emoryhealthcare.org] Sent: Friday, October 10, 2014 11:35 AM To: 'Sarah.Dysart@stdavids.com'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Another TGIF Friday... "LAB HIERARCHY" Chief Pathologist Leaps tall building in a single bound Is more powerful than a locomotive Is faster than a speeding bullet Walks on water, Gives policy to God Associate Pathologist Leaps short buildings in single bound Is more powerful than a switch engine Is just as fast as a speeding bullet Walks on water if sea is calm Talks with God The Department Head Leaps short buildings with a running start and favorable winds Is almost as powerful as a switch engine Is faster than a speeding bullet Walks on water in an indoor pool Talks with God if special request is approved Director of Laboratories Rarely clears a Quonset hut Loses tug of war with locomotive Can fire a speeding bullet Swims well Is occasionally addressed by God Associate Director of Laboratories Makes high marks on the walls when trying to leap tall buildings Is run over by locomotives Can sometimes handle a gun without inflicting self-injury Dog paddles Talks to animals Supervisor Runs into building Recognizes locomotives two out of three times Is not issued ammunition Can stay afloat with a life jacket Talks to walls Chief Technologist Falls over doorstep when trying to enter building Says "look at the choo-choo" Wets themselves with a water pistol Plays in mud puddles Mumbles to Themselves Histotechnologist Lifts tall buildings and walks under them Kicks locomotives off the tracks Catches speeding bullets in their teeth and eats them Freezes water with a single glance They are God Anonymous Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com Sent: Friday, October 10, 2014 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Another TGIF Friday... Does anyone have that thing handy that says what different positions in the histology lab do? It's like Superman, leaping buildings and such... Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Sat Oct 11 19:48:17 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sat Oct 11 19:48:25 2014 Subject: [Histonet] Another TGIF Friday... In-Reply-To: <34C22BB94729434598D767D3F4EB95E0F48E109CFD@FWDCWPMSGCMS03.hca.corpad.net> References: <34C22BB94729434598D767D3F4EB95E0F48E109CFD@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: A slightly different version: The Gospel According to the Tech TRIANGLE BIOMEDICAL SCIENCES Durham, NC 27705 (919) 477-9283 A Pathologist Leaps tall buildings in a single bound, is more powerful than a locomotive, is faster than a speeding bullet, walks on water, gives policy to God. An Internist Leaps short buildings in a single bound, is more powerful than a switch engine, is just as fast as a speeding bullet, walks on water if sea is calm, talks with God. A Surgeon Leaps short buildings with a running start, and favorable wind, is almost as powerful as a switch engine, walks on water in an indoor swimming pool, talks with God if special permission is approved. A General Practitioner Barely clears a Quonset hut, loses tug of war with a locomotive, can fire a speeding bullet, swims well, is occasionally addressed by God. A Gynecologist Makes high marks on walls when trying to leap tall buildings, is run over by a locomotive, can sometimes handle a gun without inflicting self injury, is all wet, talks to animals. A Psychiatrist Runs into buildings, recognizes locomotives two out of three times, is not issued live ammunition, can stay afloat with a life jacket, talks to walls. A Medical Student Falls over doorstep when trying to enter buildings, says ?look at the choo-choo?, wets himself with a water pistol, plays in mud puddles, mumbles to himself. A Histologist Lifts buildings and walks under them, kicks locomotives off the tracks catches speeding bullets in his/her teeth and eats them, freezes water with a single glance, is GOD. From: To: Date: 10/10/2014 08:51 AM Subject: [Histonet] Another TGIF Friday... Sent by: histonet-bounces@lists.utsouthwestern.edu Does anyone have that thing handy that says what different positions in the histology lab do? It's like Superman, leaping buildings and such... Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmoreira <@t> sidra.org Sun Oct 12 23:52:10 2014 From: jmoreira <@t> sidra.org (Joana Moreira) Date: Sun Oct 12 23:53:33 2014 Subject: [Histonet] EXAKT 312 Diamond Band Pathology Saw Message-ID: Hi everyone! I was wondering if there's anyone out there using the Exakt 312 Diamond Band Pathology Saw: http://www.exakt.com/Pathology-saw.101+M52087573ab0.0.html Is it good? Safe? Easy to operate and maintain? Not good? We are considering it and any feedback would be much appreciated. Send me a PM if you like. Many Thanks, Joana Joana Moreira Supervisor ? Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From kstoll <@t> mcw.edu Mon Oct 13 10:50:12 2014 From: kstoll <@t> mcw.edu (Stoll, Kathryn) Date: Mon Oct 13 10:50:17 2014 Subject: [Histonet] TMA help Message-ID: Hello Histonetters, I have a PI who made a very large TMA and it is half detatched from the base of the cassette. Does anyone have any tips on trying to stabilize it prior to cutting? Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Milwaukee WI 53226 Phone: 414-805-1525 Fax: 414-805-1528 From Valerie.Hannen <@t> parrishmed.com Mon Oct 13 10:50:03 2014 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Oct 13 10:51:09 2014 Subject: [Histonet] RE: need help on a cap question In-Reply-To: <9C32F30B6662D74A8419DDDB7E66656A091E865F@VHAV15MSGA1.v15.med.va.gov> References: <9C32F30B6662D74A8419DDDB7E66656A091E865F@VHAV15MSGA1.v15.med.va.gov> Message-ID: <450B7A81EDA0C54E97C53D60F00776C323373841B4@isexstore03> Kim, In our department, we assist in the collection of FNA's in radiology, the Pathologist comes there after the slides are prepared and gives a quick interpretation. If the specimen is deemed adequate or not, and if more material is requested, this is noted on the requisition and signed and dated by the Pathologist. This quick interpretation note is then also typed into the comments section of the patients Path. report. I hope this helps. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kolman, Kimberly D. Sent: Tuesday, October 07, 2014 12:55 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] need help on a cap question I could use some help clarifying this question: **NEW** 04/21/2014 ANP.11525 Tissue/Cytology Assessment Record Phase I If a statement of adequacy, preliminary diagnosis, or recommendations for additional studies is provided at the time of tissue and cytology sample collection, documentation of that statement is maintained. NOTE: Documentation might include a note in the medical record or in the final report. Is this really asking that if the ordering physician wants something in particular, it must be noted somewhere? I'm hung up on the mention of 'at the time of sample collection'............. Thanks, Kim Kim Kolman Kimberly D. Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center Histology - 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000x52537 Fax: 913-758-4193 Kim.kolman@va.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From joewalker <@t> rrmc.org Mon Oct 13 11:48:19 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Mon Oct 13 11:48:31 2014 Subject: [Histonet] RE: need help on a cap question In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C323373841B4@isexstore03> References: <9C32F30B6662D74A8419DDDB7E66656A091E865F@VHAV15MSGA1.v15.med.va.gov> <450B7A81EDA0C54E97C53D60F00776C323373841B4@isexstore03> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC186BD0E7@RRMBX03.rrmc.local> We perform something similar to Valerie. The pathologist provides a written immediate interpretation on a FNA requisition form. If the sample is insufficient, it is documented on this form. This is performed on each pass performed. The rapid interpretation is transcribed into the final pathology report. The FNA requisition is also scanned into our EMR. The rapid interpretation for each pass is required for the pathologist to bill their professional services. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, October 13, 2014 11:50 AM To: 'Kolman, Kimberly D.'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: need help on a cap question Kim, In our department, we assist in the collection of FNA's in radiology, the Pathologist comes there after the slides are prepared and gives a quick interpretation. If the specimen is deemed adequate or not, and if more material is requested, this is noted on the requisition and signed and dated by the Pathologist. This quick interpretation note is then also typed into the comments section of the patients Path. report. I hope this helps. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kolman, Kimberly D. Sent: Tuesday, October 07, 2014 12:55 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] need help on a cap question I could use some help clarifying this question: **NEW** 04/21/2014 ANP.11525 Tissue/Cytology Assessment Record Phase I If a statement of adequacy, preliminary diagnosis, or recommendations for additional studies is provided at the time of tissue and cytology sample collection, documentation of that statement is maintained. NOTE: Documentation might include a note in the medical record or in the final report. Is this really asking that if the ordering physician wants something in particular, it must be noted somewhere? I'm hung up on the mention of 'at the time of sample collection'............. Thanks, Kim Kim Kolman Kimberly D. Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center Histology - 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000x52537 Fax: 913-758-4193 Kim.kolman@va.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From awatanabe <@t> tgen.org Mon Oct 13 12:40:45 2014 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Mon Oct 13 12:40:51 2014 Subject: [Histonet] TMA help (Stoll, Kathryn) Message-ID: You can try to fill the TMA through the cassette from the bottom. Place the TMA in a mold that fits the TMA, heat it for about 2 min and then try filling the TMA from the cassette side. You can also dip the heated TMA block in a paraffin bath to fill the hole or use a plastic pipette to fill the gap. Anyway you get the paraffin to fill the hole you will need to melt the whole TMA for a bit to create a uniform block. If you have more questions give me a call. Aprill Watanabe, B.S. Laboratory Coordinator Macromolecular Analyte Processing Center (MAPC) Integrated Cancer Genomics Division Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Office: 602-343-8822 Fax: 602-343-8840 Cell: 602-481-8654 email: awatanabe@tgen.org website: www.tgen.org From sbaldwin <@t> mhhcc.org Mon Oct 13 13:35:36 2014 From: sbaldwin <@t> mhhcc.org (Baldwin, Kathy) Date: Mon Oct 13 13:35:42 2014 Subject: [Histonet] FW: H Pylori IHC In-Reply-To: References: Message-ID: Hi all My pathologist would like our techs to do H pylori IHC stains on all stomach, GE Junction and esophagus BX's. What I was wondering, if there were any indication in Regulations or anything that we couldn't do this? Any advice would be greatly appreciated :) S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbaldwin@mhhcc.org :) "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" Vision Statement: We are committed to being the preferred health and wellness provider; transforming lives through faith based, compassionate care. From Wanda.Smith <@t> HCAhealthcare.com Mon Oct 13 13:49:29 2014 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Oct 13 13:49:36 2014 Subject: [Histonet] RE: H Pylori IHC In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2801E3B9E6@NADCWPMSGCMS03.hca.corpad.net> Kathy, My understanding is CMS (Medicare) has ruled that h.Pylori stains cannot be a standing order. The Pathologist must look at the H&E's first and deem the stain is needed based on the inflammation. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Baldwin, Kathy Sent: Monday, October 13, 2014 2:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] FW: H Pylori IHC Hi all My pathologist would like our techs to do H pylori IHC stains on all stomach, GE Junction and esophagus BX's. What I was wondering, if there were any indication in Regulations or anything that we couldn't do this? Any advice would be greatly appreciated :) S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbaldwin@mhhcc.org :) "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" Vision Statement: We are committed to being the preferred health and wellness provider; transforming lives through faith based, compassionate care. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne <@t> rwjuh.edu Mon Oct 13 14:20:23 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Mon Oct 13 14:20:31 2014 Subject: [Histonet] RE: H Pylori IHC In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2801E3B9E6@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2801E3B9E6@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED2369@vap1014.win.rwjuh.edu> You might be able to get around this by doing the IHC on all of these as requested, but only charging for those which are indicated as being medically necessary. This could benefit your TAT, if that's the reason the pathologist wants these done routinely. The pathologist would also have to agree not to bill for the PC of this test in these cases also. You might want to do a retrospective study and determine if this was done what the financial impact would have been. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Monday, October 13, 2014 2:49 PM To: sbaldwin@mhhcc.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: H Pylori IHC Kathy, My understanding is CMS (Medicare) has ruled that h.Pylori stains cannot be a standing order. The Pathologist must look at the H&E's first and deem the stain is needed based on the inflammation. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Baldwin, Kathy Sent: Monday, October 13, 2014 2:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] FW: H Pylori IHC Hi all My pathologist would like our techs to do H pylori IHC stains on all stomach, GE Junction and esophagus BX's. What I was wondering, if there were any indication in Regulations or anything that we couldn't do this? Any advice would be greatly appreciated :) S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbaldwin@mhhcc.org :) "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" Vision Statement: We are committed to being the preferred health and wellness provider; transforming lives through faith based, compassionate care. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Oct 13 14:32:26 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Oct 13 14:32:31 2014 Subject: [Histonet] RE: H Pylori IHC In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED2369@vap1014.win.rwjuh.edu> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA2801E3B9E6@NADCWPMSGCMS03.hca.corpad.net> <59E09A4EFBD3F349BD75FDAE8AFB0F24ED2369@vap1014.win.rwjuh.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E3916999F54C3@IBMB7Exchange.digestivespecialists.com> You might find that the financial impact wouldn't be that great. There is a reason they are doing an EGD, most commonly for gastritis which is a valid reason to look for H pylori. There is a pretty standard list of ICD 9 codes that would trigger the justification for doing an H pylori. Also, you're going to one of two things when you have a stomach biopsy. You will either cut extra slides, (time and material there) or you will wait until its ordered (loss of TAT). So, if you are going to do a retrospective study you need to include that in your review. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Monday, October 13, 2014 3:20 PM To: 'Wanda.Smith@HCAhealthcare.com'; sbaldwin@mhhcc.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: H Pylori IHC You might be able to get around this by doing the IHC on all of these as requested, but only charging for those which are indicated as being medically necessary. This could benefit your TAT, if that's the reason the pathologist wants these done routinely. The pathologist would also have to agree not to bill for the PC of this test in these cases also. You might want to do a retrospective study and determine if this was done what the financial impact would have been. Good luck. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Monday, October 13, 2014 2:49 PM To: sbaldwin@mhhcc.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: H Pylori IHC Kathy, My understanding is CMS (Medicare) has ruled that h.Pylori stains cannot be a standing order. The Pathologist must look at the H&E's first and deem the stain is needed based on the inflammation. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Baldwin, Kathy Sent: Monday, October 13, 2014 2:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] FW: H Pylori IHC Hi all My pathologist would like our techs to do H pylori IHC stains on all stomach, GE Junction and esophagus BX's. What I was wondering, if there were any indication in Regulations or anything that we couldn't do this? Any advice would be greatly appreciated :) S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbaldwin@mhhcc.org :) "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" Vision Statement: We are committed to being the preferred health and wellness provider; transforming lives through faith based, compassionate care. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Mon Oct 13 14:43:42 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Oct 13 14:43:47 2014 Subject: [Histonet] RE: H Pylori IHC In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355D5023@HHCEXCHMB03.hhcsystem.org> I think the real question here is, "Why do your pathologists want H. pylori IHC on all of these specimens?" If it's "TAT, maybe something can be done to improve it so that you not have to do all this "unnecessary" work. We only order H. pylori IHC on those cases that show the appropriate inflammatory background and organisms cannot be identified on H&E. Also, IHC can be helpful when patients have been treated for H. pylori and, once again, organisms cannot be identified on H&E. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Baldwin, Kathy Sent: Monday, October 13, 2014 2:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] FW: H Pylori IHC Hi all My pathologist would like our techs to do H pylori IHC stains on all stomach, GE Junction and esophagus BX's. What I was wondering, if there were any indication in Regulations or anything that we couldn't do this? Any advice would be greatly appreciated :) S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbaldwin@mhhcc.org :) "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" Vision Statement: We are committed to being the preferred health and wellness provider; transforming lives through faith based, compassionate care. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From BMolinari <@t> texasheart.org Tue Oct 14 07:04:23 2014 From: BMolinari <@t> texasheart.org (Betsy Molinari) Date: Tue Oct 14 07:04:31 2014 Subject: [Histonet] white plastic scrapers Message-ID: Hi all, Where can I purchase the whit plastic scrapers used to scrape the paraffin off embedding centers and other surfaces. I believe ours went out with the trash. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] From bcooper <@t> chla.usc.edu Tue Oct 14 07:37:50 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Oct 14 07:37:56 2014 Subject: [Histonet] white plastic scrapers In-Reply-To: References: Message-ID: You know what works better? A small, metal drywall taping/mud knife-maybe 3-4 inches. They're more flexible than the plastic scrapers, and I think they actually pick up more paraffin per pass. You can pick these up at any hardware store. Thanks, Brian Cooper, HT (ASCP) Histology Supervisor, Path & Lab Medicine Children's Hospital, Los Angeles Sent from my Galaxy S3, so please forgive any weird typos . . . -----Original Message----- From: Betsy Molinari [BMolinari@texasheart.org] Received: Tuesday, 14 Oct 2014, 5:08AM To: Histonet@lists.utsouthwestern.edu [Histonet@lists.utsouthwestern.edu] Subject: [Histonet] white plastic scrapers Hi all, Where can I purchase the whit plastic scrapers used to scrape the paraffin off embedding centers and other surfaces. I believe ours went out with the trash. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From mbell <@t> flagshipbio.com Tue Oct 14 07:46:00 2014 From: mbell <@t> flagshipbio.com (Mandy Bell) Date: Tue Oct 14 07:46:12 2014 Subject: [Histonet] CD31 in rabbit tissue Message-ID: <50d0b917c1754adb93e945aa9f97e0b4@DM2PR0701MB1018.namprd07.prod.outlook.com> Hi, We are trying to run CD31 in rabbit tissue. We've tried a couple of different antibodies with no luck. Hoping someone can point me in the right direction- does anyone have a CD31 they have had success with in rabbit tissue? Thanks, Mandy Bell, HTL(ASCP) From fbozkurt <@t> gmail.com Tue Oct 14 07:56:22 2014 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Tue Oct 14 07:56:33 2014 Subject: [Histonet] CD31 in rabbit tissue In-Reply-To: <50d0b917c1754adb93e945aa9f97e0b4@DM2PR0701MB1018.namprd07.prod.outlook.com> References: <50d0b917c1754adb93e945aa9f97e0b4@DM2PR0701MB1018.namprd07.prod.outlook.com> Message-ID: Hi, Santacruz Goat sc-1506 works well on rabbit tissues. Good lucks. On Tue, Oct 14, 2014 at 3:46 PM, Mandy Bell wrote: > Hi, > > We are trying to run CD31 in rabbit tissue. We've tried a couple of > different antibodies with no luck. Hoping someone can point me in the > right direction- does anyone have a CD31 they have had success with in > rabbit tissue? > > Thanks, > > Mandy Bell, HTL(ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 Asst. Prof. Dr. Mehmet Fatih BOZKURT Manager of Veterinary Diagnostic Laboratory Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16300-16301 vetanaliz@aku.edu.tr From BDeBrosse-Serra <@t> isisph.com Tue Oct 14 08:34:16 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Oct 14 08:34:37 2014 Subject: [Histonet] RE: CD31 in rabbit tissue In-Reply-To: <50d0b917c1754adb93e945aa9f97e0b4@DM2PR0701MB1018.namprd07.prod.outlook.com> References: <50d0b917c1754adb93e945aa9f97e0b4@DM2PR0701MB1018.namprd07.prod.outlook.com> Message-ID: We use CD31 from Spring BioScience (SP38). Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mandy Bell Sent: Tuesday, October 14, 2014 5:46 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 in rabbit tissue Hi, We are trying to run CD31 in rabbit tissue. We've tried a couple of different antibodies with no luck. Hoping someone can point me in the right direction- does anyone have a CD31 they have had success with in rabbit tissue? Thanks, Mandy Bell, HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From klaus.dern44 <@t> gmail.com Tue Oct 14 08:59:42 2014 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Tue Oct 14 08:59:45 2014 Subject: [Histonet] THICK AND THIN SECTIONS ? Message-ID: If you are using one of the following microtomes and the advance mechanism is worn out ( too much play between spindle and spindle nut ), you could be faced with purchasing a new microtome. REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1800 Cryostat SAKURA - SRM 200 These models are no longer supported by the manufacturer, (no parts availability). Rather than replacing these excellent instruments, I have a PERMANENT solution to fix this problem. For information please contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com From Joyce.Weems <@t> emoryhealthcare.org Tue Oct 14 09:43:37 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Oct 14 09:43:56 2014 Subject: [Histonet] RE: white plastic scrapers In-Reply-To: References: Message-ID: I've seen them at Bed Bath and Beyond if you're talking about the little ones about 2 1/2 or 3 in squarish ones. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Betsy Molinari Sent: Tuesday, October 14, 2014 8:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] white plastic scrapers Hi all, Where can I purchase the whit plastic scrapers used to scrape the paraffin off embedding centers and other surfaces. I believe ours went out with the trash. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Milton.Gomez <@t> nyumc.org Tue Oct 14 09:55:10 2014 From: Milton.Gomez <@t> nyumc.org (Gomez, Milton) Date: Tue Oct 14 09:55:19 2014 Subject: [Histonet] RE: Gram staining In-Reply-To: References: <77F52EFAB8B1694B885E277C48FCD0F67BA7FF02@GHSEXMBX1W8K1V.geisinger.edu>, Message-ID: <2B0D905D71C9AA4B859B99F404A6C4A11116C17D@MSGWCDCPMB27.nyumc.org> we use 5ml Picric Acid to 50 ml acetone and it works very well. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cooper, Brian [bcooper@chla.usc.edu] Sent: Thursday, October 09, 2014 3:48 PM To: Bitting, Angela K.; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Gram staining We use the McDonald's Gram Stain kit from American Master Tech, which employs the use of a Modified Tartrazine counterstain. Both our docs and techs love our Gram stains . . . Here's the link to the product info. http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=KTMGS Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Thursday, October 09, 2014 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gram staining Our new Pathologist would like to see more yellow in the background of our Gram stain. We use picric acid-acetone currently, which looks more pink than yellow most times. So I'm wondering if a method using a Tartrazine counterstain would be better. Any thoughts? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Tue Oct 14 10:25:41 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Oct 14 10:25:48 2014 Subject: [Histonet] white plastic scrapers In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE25BFF550317@MERCERMAIL.MercerU.local> Metal scrapers may remove some of the ceramic coatings on embedding centers, they do have plastic putty knives at Lowes or Home Depot or where ever you get hardware. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Tuesday, October 14, 2014 8:38 AM To: Histonet@lists.utsouthwestern.edu; BMolinari@texasheart.org Subject: RE: [Histonet] white plastic scrapers You know what works better? A small, metal drywall taping/mud knife-maybe 3-4 inches. They're more flexible than the plastic scrapers, and I think they actually pick up more paraffin per pass. You can pick these up at any hardware store. Thanks, Brian Cooper, HT (ASCP) Histology Supervisor, Path & Lab Medicine Children's Hospital, Los Angeles Sent from my Galaxy S3, so please forgive any weird typos . . . -----Original Message----- From: Betsy Molinari [BMolinari@texasheart.org] Received: Tuesday, 14 Oct 2014, 5:08AM To: Histonet@lists.utsouthwestern.edu [Histonet@lists.utsouthwestern.edu] Subject: [Histonet] white plastic scrapers Hi all, Where can I purchase the whit plastic scrapers used to scrape the paraffin off embedding centers and other surfaces. I believe ours went out with the trash. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Tue Oct 14 11:09:42 2014 From: suetp918 <@t> comcast.net (Sue) Date: Tue Oct 14 11:09:58 2014 Subject: [Histonet] white plastic scrapers In-Reply-To: References: Message-ID: <1634971960.1600426.1413302982009.JavaMail.root@comcast.net> Home Depot or Lowes sells plastic scrappers in the paint area.? I cannot see why we would pay top dollar for this item.? I also purchase brushed to clean paraffin off microtomes at the craft store.? 50 cents each is much better than 5 dollars. ? Sue Paturzo TJUH From Dorothy.L.Webb <@t> HealthPartners.Com Tue Oct 14 11:13:00 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Oct 14 11:13:04 2014 Subject: [Histonet] recycled formalin Message-ID: <64be8f5d98be4829b49c40998f44d140@HPEXCH05.HealthPartners.int> How does everyone handle the use of recycled formalin in regard to tissue processors? OK to use on the processors? We have just recently installed the Creative Waste formalin recycler and I am looking for guidelines from those who have experience in this area. Much thanks, Dorothy Webb (Regions Histology, St. Paul, MN) ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From algranth <@t> email.arizona.edu Tue Oct 14 11:22:43 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Oct 14 11:22:48 2014 Subject: [Histonet] RE: white plastic scrapers In-Reply-To: References: Message-ID: Southeast Pathology was giving out scrapers at NSH this year but in case you didn't get one - go to the cooking section of Target or Walmart and see if they have a scraper to use on teflon surfaces. A squeegee works good on molten wax surfaces too. Otherwise, a snow scraper might work too. Retired but lurking, Andi G. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Betsy Molinari [BMolinari@texasheart.org] Sent: Tuesday, October 14, 2014 5:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] white plastic scrapers Hi all, Where can I purchase the whit plastic scrapers used to scrape the paraffin off embedding centers and other surfaces. I believe ours went out with the trash. Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] From Lynne.Bell <@t> cvmc.org Tue Oct 14 11:35:21 2014 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Tue Oct 14 11:35:27 2014 Subject: [Histonet] Slides from antibody validation Message-ID: Following antibody validation studies, do you keep your slides accessible for CAP to peruse during inspection? Has anyone had CAP actually ask to see your slides? I feel that keeping the validation forms, signed off by the medical director, is sufficient, but I would like other opinions. As always, thanks for any and all guidance! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT? 05641 802-371-4923 From lcolbert <@t> pathmdlabs.com Tue Oct 14 12:21:08 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Oct 14 12:21:13 2014 Subject: [Histonet] Labeling Errors Message-ID: <12ECD7346266D74691EC2BFC75285E453811740A@BFL323E10.pathmdlabs.local> Is there a national average or benchmark for "acceptable" labeling errors in Histology? Laurie Colbert HT (ASCP) From joewalker <@t> rrmc.org Tue Oct 14 12:57:30 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Oct 14 12:57:36 2014 Subject: [Histonet] RE: Slides from antibody validation In-Reply-To: References: Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC186C11A7@RRMBX03.rrmc.local> On a recent CAP inspection, we did have an inspector ask to see our slides from our HER2 validation. For the past 5 years, we have kept all of the slides from our validation studies along with the paperwork in a notebook. These glass slide holders have worked well for keeping everything together in one place for inspections: http://www.rochester100.com/store/p/195-SL-3-182.aspx Interestingly enough, on a recent Joint Commission inspection last year, an inspector made a similar request to see an example of an IHC that we had validated. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Tuesday, October 14, 2014 12:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Slides from antibody validation Following antibody validation studies, do you keep your slides accessible for CAP to peruse during inspection? Has anyone had CAP actually ask to see your slides? I feel that keeping the validation forms, signed off by the medical director, is sufficient, but I would like other opinions. As always, thanks for any and all guidance! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From Timothy.Morken <@t> ucsfmedctr.org Tue Oct 14 12:57:46 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Oct 14 12:57:52 2014 Subject: [Histonet] RE: Labeling Errors In-Reply-To: <12ECD7346266D74691EC2BFC75285E453811740A@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E453811740A@BFL323E10.pathmdlabs.local> Message-ID: <761E2B5697F795489C8710BCC72141FF367AFA6E@ex07.net.ucsf.edu> Laurie, The question to ask is, what is ACHIEVABLE with the technology you have in place? The term is ALRA or ALRP...As Low as Reasonably Achievable, or Possible. For instance, when we hand-wrote slide labels, and used offline cassette printers we accepted that we would have a rather high rate of human errors. Same with hand-applying labels after staining. With barcoding, printing cassettes directly from the LIS, printing and applying labels at the microtome our error rate is down by an order magnitude, but occasional errors still occur due to humans "shortcutting" the system, or faults in the system itself (ie, not flagging duplicate labels scanned). None of those errors are acceptable but we still need to figure out how to design the system to prevent them. The papers below give numbers to this problem. The patient ID error without automation came out to 4+ per 1000 specimens. The general error rate was/is: 1/100 with all hand-written workflow 1/1000 with LIS printing of cassettes and labels, but human-applied 1/10,000 with barcoding and single piece workflow throughout the system and interfaces to instruments (stainers) Makary MA et al. Surgical specimen identification errors.... Surgery 2007 Apr;141(4):450-5 Nakhleh RE, et al. Amended reports ... Q-probes study of 1,667,547 accessioned cases ... Arch Pathol Lab Med. 1998 Apr;122(4):303-9 Resar RK. Making noncatastrophic health care processes more reliable... Health Serv Res. 2006; 41:1677-1689. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, October 14, 2014 10:21 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Labeling Errors Is there a national average or benchmark for "acceptable" labeling errors in Histology? Laurie Colbert HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Oct 14 13:37:26 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Tue Oct 14 13:37:30 2014 Subject: [Histonet] RE: Labeling Errors In-Reply-To: <761E2B5697F795489C8710BCC72141FF367AFA6E@ex07.net.ucsf.edu> References: <12ECD7346266D74691EC2BFC75285E453811740A@BFL323E10.pathmdlabs.local>, <761E2B5697F795489C8710BCC72141FF367AFA6E@ex07.net.ucsf.edu> Message-ID: Thanks Tim, these references are VERY helpful. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: lcolbert@pathmdlabs.com; histonet@lists.utsouthwestern.edu > Date: Tue, 14 Oct 2014 17:57:46 +0000 > CC: > Subject: [Histonet] RE: Labeling Errors > > Laurie, > > The question to ask is, what is ACHIEVABLE with the technology you have in place? The term is ALRA or ALRP...As Low as Reasonably Achievable, or Possible. > > For instance, when we hand-wrote slide labels, and used offline cassette printers we accepted that we would have a rather high rate of human errors. Same with hand-applying labels after staining. > > With barcoding, printing cassettes directly from the LIS, printing and applying labels at the microtome our error rate is down by an order magnitude, but occasional errors still occur due to humans "shortcutting" the system, or faults in the system itself (ie, not flagging duplicate labels scanned). None of those errors are acceptable but we still need to figure out how to design the system to prevent them. > > The papers below give numbers to this problem. The patient ID error without automation came out to 4+ per 1000 specimens. > > The general error rate was/is: > 1/100 with all hand-written workflow > 1/1000 with LIS printing of cassettes and labels, but human-applied > 1/10,000 with barcoding and single piece workflow throughout the system and interfaces to instruments (stainers) > > Makary MA et al. Surgical specimen identification errors.... Surgery 2007 Apr;141(4):450-5 > Nakhleh RE, et al. Amended reports ... Q-probes study of 1,667,547 accessioned cases ... Arch Pathol Lab Med. 1998 Apr;122(4):303-9 > Resar RK. Making noncatastrophic health care processes more reliable... Health Serv Res. 2006; 41:1677-1689. > > > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Tuesday, October 14, 2014 10:21 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Labeling Errors > > Is there a national average or benchmark for "acceptable" labeling errors in Histology? > > Laurie Colbert HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Oct 14 14:06:05 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Tue Oct 14 14:06:10 2014 Subject: [Histonet] RE: white plastic scrapers In-Reply-To: References: , Message-ID: I believe at some point I got some at a paint shop like Sherwin Williams. Carry mine with me in my "box of tools", works great. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: algranth@email.arizona.edu > To: BMolinari@texasheart.org; Histonet@lists.utsouthwestern.edu > Date: Tue, 14 Oct 2014 16:22:43 +0000 > CC: > Subject: [Histonet] RE: white plastic scrapers > > Southeast Pathology was giving out scrapers at NSH this year but in case you didn't get one - go to the cooking section of Target or Walmart and see if they have a scraper to use on teflon surfaces. A squeegee works good on molten wax surfaces too. Otherwise, a snow scraper might work too. > > Retired but lurking, > Andi G. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Betsy Molinari [BMolinari@texasheart.org] > Sent: Tuesday, October 14, 2014 5:04 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] white plastic scrapers > > Hi all, > Where can I purchase the whit plastic scrapers used to scrape the paraffin off embedding centers and other surfaces. I believe ours went out with the trash. > Thanks. > > Betsy Molinari HT(ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave > Houston,TX 77030 > 832-355-6524 (lab) > 832-355-6812 (fax) > > > > > > > > Betsy Molinari > Senior Histology Research Technician > 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org > > > > 6770 Bertner Ave., MC 1-283, Houston, TX 77030 > > > > [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Wed Oct 15 02:54:04 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Wed Oct 15 02:54:36 2014 Subject: [Histonet] RE: Slides from antibody validation In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC186C11A7@RRMBX03.rrmc.local> References: <3C2378778400AD448ADA6FD6BDB7CCCC186C11A7@RRMBX03.rrmc.local> Message-ID: <008e01cfe84d$32376250$96a626f0$@rowaihi@alborglaboratories.com> Hi Lynne Bell I also keep all my slides even blocks which was used for validation, the CAP inspectors sometimes request to see them. I agree with you Joe W. Walker I am also using the same Slides Holder Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, October 14, 2014 8:58 PM To: Bell, Lynne; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Slides from antibody validation On a recent CAP inspection, we did have an inspector ask to see our slides from our HER2 validation. For the past 5 years, we have kept all of the slides from our validation studies along with the paperwork in a notebook. These glass slide holders have worked well for keeping everything together in one place for inspections: http://www.rochester100.com/store/p/195-SL-3-182.aspx Interestingly enough, on a recent Joint Commission inspection last year, an inspector made a similar request to see an example of an IHC that we had validated. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Tuesday, October 14, 2014 12:35 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Slides from antibody validation Following antibody validation studies, do you keep your slides accessible for CAP to peruse during inspection? Has anyone had CAP actually ask to see your slides? I feel that keeping the validation forms, signed off by the medical director, is sufficient, but I would like other opinions. As always, thanks for any and all guidance! Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Wed Oct 15 10:27:38 2014 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Oct 15 10:27:46 2014 Subject: [Histonet] FISH Applications Specialist Message-ID: <030c01cfe88c$8e948fc0$abbdaf40$@personifysearch.com> Good morning! We are searching for an Applications Specialist to join a world leading cancer diagnostics company. The position will focus on FISH and cytogenetics and will be based in the IL area. The position will provide internal support as well as provide customer support for the West Coast. If you have experience with FISH and would be interested in catching up to discuss in more detail, please e-mail directly at mw@personifysearch.com Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com From ltougas <@t> dawsoncollege.qc.ca Wed Oct 15 15:13:30 2014 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Wed Oct 15 15:13:48 2014 Subject: [Histonet] Surgipath Tribune Stainer In-Reply-To: <455897B94CF3A44F81F727160F23FB112CED9A93@DC229.ad.dawsoncollege.qc.ca> References: <455897B94CF3A44F81F727160F23FB112CECA6B9@DC229.ad.dawsoncollege.qc.ca>, <455897B94CF3A44F81F727160F23FB112CECA6D7@DC229.ad.dawsoncollege.qc.ca>, <455897B94CF3A44F81F727160F23FB112CED9A93@DC229.ad.dawsoncollege.qc.ca> Message-ID: <455897B94CF3A44F81F727160F23FB112CED9FED@DC229.ad.dawsoncollege.qc.ca> Hi Histo world! Would anyone be familiar with the Surgipath Tribune Stainer model HCS 33? I have been informed that there may still be some around, in Ontario (Windsor?) maybe. It is a vertical model and it just died on us and Leica does not support it anymore. The problem is that there is no signal on the screen when we turn it on so it cannot be started. We were wondering if anything can still be done or if we just bury it! The last software version is from 2003. I have been trying to attach a picture but Histonet would not let it go through. Thank you in advance, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal, Qc, Canada From JMacDonald <@t> mtsac.edu Wed Oct 15 18:36:09 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Oct 15 19:08:23 2014 Subject: [Histonet] RDO Message-ID: Does anyone know what the "O" signifies in RDO? Optimal? Thanks, Jennifer From DSiena <@t> statlab.com Wed Oct 15 19:31:38 2014 From: DSiena <@t> statlab.com (Debra Siena) Date: Wed Oct 15 19:31:47 2014 Subject: [Histonet] RDO In-Reply-To: References: Message-ID: <5A5C7ACA-C832-4084-93F8-B67A74DB26A8@statlab.com> At NSH Mark Ostermeier told us at the awards banquet that it stood for the names of his parents, Donald and Rosemary Ostermeier who were the inventors of the product that was originally designed to clean pipes. It was interesting to learn that bit of trivia. Sent from my iPhone > On Oct 15, 2014, at 6:10 PM, "Jennifer MacDonald" wrote: > > Does anyone know what the "O" signifies in RDO? Optimal? > > Thanks, > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Thu Oct 16 09:22:31 2014 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Oct 16 09:22:42 2014 Subject: [Histonet] =?iso-8859-1?q?Shandon_Excelsior_ES=AE?= Message-ID: What do people think about the Shandon Excelsior processor? I currently have the old Miles-Sakura VIP and we love it but we are looking for another processor so we can have 2 processors for fatty tissue. I am ok with getting another refurbished VIP. I have excellent service contracted with a Bio Medical repair company they do all my PMs and repairs. I'm trying to save money so I don't want to spend it where I don't really need it. My mind frame is 'If its not broke don't fix it" Any thoughts on this would be welcomed. Thanks, Cheri Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rsrichmond <@t> gmail.com Thu Oct 16 12:11:32 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Oct 16 12:11:36 2014 Subject: [Histonet] Re: RDO Message-ID: RDO is a popular decalcifier, though I haven't seen it in use for several years now. Here's its present status: http://www.rdodecal.com/p_rdo.html I never could see the point of using this stuff - totally secret formula, murky solution that continues to precipitate black crud you have to filter out just before using it. Does RDO support immunostaining? Bob Richmond Samurai Pathologist Maryville TN From bcdukes <@t> lexhealth.org Thu Oct 16 12:33:55 2014 From: bcdukes <@t> lexhealth.org (Blake Taylor) Date: Thu Oct 16 12:34:00 2014 Subject: [Histonet] Trichrome Kit Failures Message-ID: Is anyone having Trichrome Kit Failures on the Ventana Benchmark Special Stainers. We are running into Kits that present with 10 stains left but we are finding the mordant has actually run out and the stains will be all blue. There is no way of knowing this will happen ahead of time. The machine does not have an alert. This has happened with different kits from different lot numbers. Ventana is researching but they are telling us that no one else is experiencing this problem. If anyone has run into similar problems I would love to hear from you. Thanks Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From PAMarcum <@t> uams.edu Thu Oct 16 13:47:30 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Oct 16 13:47:40 2014 Subject: [Histonet] =?windows-1252?q?RE=3A_Shandon_Excelsior_ES=AE?= In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012528492F@Mail2Node2.ad.uams.edu> We have four Excelsior processors and love them. We had some very old VIPs that we replaced with them over the past few years. They are safer as the reagent handling and changing is quick and simple. We dump the alcohol when the unit hygrometer (and the techs) see the percentage is low and ready to change, by allowing the unit to dump the dirty alcohol into an empty container in the lower half of the cabinet. Then we remove the dirty bottle and cap it; while putting in a clean alcohol to be picked up and pulled in when the correct place is reached in the program. It is the same with the xylene exchanges. It dumps the paraffin when it is contaminated and you remove it, dump and reload in the empty chamber. You set the number of uses for formalin and it counts down to a change and does the same dump and change out routine. It saves us about 70% or more on reagent costs and would be higher if we were not using a Leica ASP 300s for our bone marrows. It is easy to use and program and no they don?t pay me!! It is safety first and less exposure to alcohol and xylene fumes is the real plus I wanted for the lab for everyone. (No one complains anymore when we change reagents about the smell. We share a large area with 4 other lab areas who are very picky!) The rest we just learned to love. We have had a few services calls however; Thermo answered fast and we up and running very quickly. We use them every day and they are workhorses. We have one for placenta and autopsy only, two for routine surgicals and one for biopsies only. We also run biopsies and RUSH specimens during the day if they are available. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, October 16, 2014 9:23 AM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Shandon Excelsior ES? What do people think about the Shandon Excelsior processor? I currently have the old Miles-Sakura VIP and we love it but we are looking for another processor so we can have 2 processors for fatty tissue. I am ok with getting another refurbished VIP. I have excellent service contracted with a Bio Medical repair company they do all my PMs and repairs. I'm trying to save money so I don't want to spend it where I don't really need it. My mind frame is 'If its not broke don't fix it" Any thoughts on this would be welcomed. Thanks, Cheri Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mundayscott <@t> gmavt.net Thu Oct 16 14:05:23 2014 From: mundayscott <@t> gmavt.net (scott munday) Date: Thu Oct 16 14:05:28 2014 Subject: [Histonet] Refurbished Olympus BX Microscopes Message-ID: Is your lab in need of a new microscope? We have several Olympus BX40 and BX41 compound microscopes in stock! and offer a 1 year warranty on all scopes. All microscopes are fully refurbished and are priced at 40% off list. The scopes are serviced by an Authorized Olympus Service Tech before sold. Please Email or call with questions. -- Scott Munday Munday Scientific Instrument Service 90 Misha Lane Sanford, NC 27330 Phone: 919-775-5596 Fax: 919-776-9566 From lcolbert <@t> pathmdlabs.com Thu Oct 16 13:59:57 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Thu Oct 16 14:10:06 2014 Subject: [Histonet] Storage of Glacial Acetic Acid Message-ID: <12ECD7346266D74691EC2BFC75285E4538117BF7@BFL323E10.pathmdlabs.local> How do you store GAA? As I recall, it should not be stored with other acids in an acid cabinet. I'm think it should be stored with flammables?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From ludlow <@t> HHSC.CA Thu Oct 16 14:24:12 2014 From: ludlow <@t> HHSC.CA (Ludlow Patricia) Date: Thu Oct 16 14:25:24 2014 Subject: [Histonet] PPE for Fresh Tissues Message-ID: Hi all I have a question for everyone pertaining to handling fresh tissue (ie: lung or lymphnode tissue for open lung or lymphoma protocols) that may be known or suspect for TB. When handling fresh tissue, what type of PPE do you wear (ie: gloves, labcoat, N95 mask, eye shield, goggles other?) and do you handle it in a grossing hood or do you use a Biological Safety Cabinet? Do you have special engineering controls in place? Also, is staff routinely given TB tests at your hospital? Thank you Pat Ludlow Technical Specialist/Supervisor Histology HRLMP 905-527-0271 Ext. 46181 (HGH) 905-522-1155 Ext. 35954 (SJH) pager: dial ext. 75070, enter 8837 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From NMP <@t> stowers.org Thu Oct 16 15:40:09 2014 From: NMP <@t> stowers.org (Marsh, Nannette) Date: Thu Oct 16 15:40:17 2014 Subject: [Histonet] RE: Trichrome Kit Failures In-Reply-To: References: Message-ID: <7A23A5A43C06284686B4868E886775300EC83599@MBSRV02.sgc.loc> Hello Blake, We had a similar incident happen in our lab using a Trichrome Kit. The muscle from the mouse heart stained blue instead of scarlet red. We found that it was not the mordant but it was the Gomori's stain had expired. Instead of ordering a new kit, we made fresh stains in house and used the picric acid mordant from the kit. The sample stained perfectly and correctly. I know that you are using a kit for the Ventana Stainer so I understand that our situations are not exactly the same but I thought perhaps our experience might be of some help to you. Nanne Marsh HT ASCP -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blake Taylor Sent: Thursday, October 16, 2014 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome Kit Failures Is anyone having Trichrome Kit Failures on the Ventana Benchmark Special Stainers. We are running into Kits that present with 10 stains left but we are finding the mordant has actually run out and the stains will be all blue. There is no way of knowing this will happen ahead of time. The machine does not have an alert. This has happened with different kits from different lot numbers. Ventana is researching but they are telling us that no one else is experiencing this problem. If anyone has run into similar problems I would love to hear from you. Thanks Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis <@t> baylorhealth.edu Thu Oct 16 16:00:27 2014 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Thu Oct 16 16:00:32 2014 Subject: [Histonet] Histology Tracking systems Message-ID: <2572B4D63B62E64A8078D8BBE34D407801BB5BAE@BHDASVEXML2.bhcs.pvt> I know this might be a strange question but, do any Histology Labs out in Histoland use a keyboard and mouse at Embedding and Microtomy to perform tracking, slide label printing and Quality Assurance recording. Thanks in advance for your replies. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 16 16:06:08 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Oct 16 16:06:15 2014 Subject: [Histonet] RE: Histology Tracking systems In-Reply-To: <2572B4D63B62E64A8078D8BBE34D407801BB5BAE@BHDASVEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D407801BB5BAE@BHDASVEXML2.bhcs.pvt> Message-ID: <761E2B5697F795489C8710BCC72141FF367B0144@ex07.net.ucsf.edu> Donna, we have touch screens, but our application is not fully touch screen friendly yet, so we have mini-keyboards that include a trackpad. They work well for login, choosing some items on the screen etc. They are not really meant for extensive use. 99% of the interaction is achieved simply by scanning a block or slide and using the touch screen as much as possible. the keyboard is just for that one percent in which the screen does not work so well. We do all QA recording on paper at the microtome and embedding center, and then computer entry later. Again, the system is not set up well for that aspect yet. If you are thinking of entering barcodes manually (you can do that if you know the ID) , yes, you can do it, it just slows things down a lot. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, October 16, 2014 2:00 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Histology Tracking systems I know this might be a strange question but, do any Histology Labs out in Histoland use a keyboard and mouse at Embedding and Microtomy to perform tracking, slide label printing and Quality Assurance recording. Thanks in advance for your replies. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 16 16:12:40 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Oct 16 16:12:49 2014 Subject: [Histonet] RE: Histology Tracking systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B0144@ex07.net.ucsf.edu> References: <2572B4D63B62E64A8078D8BBE34D407801BB5BAE@BHDASVEXML2.bhcs.pvt> <761E2B5697F795489C8710BCC72141FF367B0144@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF367B0158@ex07.net.ucsf.edu> Got rushed and sent too soon. I meant to say: If you are thinking of entering barcodes manually (you can do that if you know the ID) , yes, you can do it, it just slows things down a lot and you WILL have labeling errors because of mis-entered codes. And it can be problematic to find out what the code is unless you are scanning into a text editor to see it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 16, 2014 2:06 PM To: 'Willis, Donna G.'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Histology Tracking systems Donna, we have touch screens, but our application is not fully touch screen friendly yet, so we have mini-keyboards that include a trackpad. They work well for login, choosing some items on the screen etc. They are not really meant for extensive use. 99% of the interaction is achieved simply by scanning a block or slide and using the touch screen as much as possible. the keyboard is just for that one percent in which the screen does not work so well. We do all QA recording on paper at the microtome and embedding center, and then computer entry later. Again, the system is not set up well for that aspect yet. If you are thinking of entering barcodes manually (you can do that if you know the ID) , yes, you can do it, it just slows things down a lot. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, October 16, 2014 2:00 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Histology Tracking systems I know this might be a strange question but, do any Histology Labs out in Histoland use a keyboard and mouse at Embedding and Microtomy to perform tracking, slide label printing and Quality Assurance recording. Thanks in advance for your replies. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Thu Oct 16 16:16:34 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Oct 16 16:16:41 2014 Subject: [Histonet] RE: Histology Tracking systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B0144@ex07.net.ucsf.edu> References: <2572B4D63B62E64A8078D8BBE34D407801BB5BAE@BHDASVEXML2.bhcs.pvt> <761E2B5697F795489C8710BCC72141FF367B0144@ex07.net.ucsf.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA320125284A07@Mail2Node2.ad.uams.edu> We do about the same thing with the embedding and sectioning. The computers are touch screen and some people use them, others use the pad on the keyboard, the rest asked for mice. We have no way to get the information we need for sectioning from the system so, we have to do the paperwork by hand. The system we have was created by engineers who thought we should do processing, embedding and staining, then deliver the slides. They missed sectioning completely and say they can't add it now. If you are on check out you get credit for sectioning, staining and delivering every slide cut that day. Just doesn't work well for workload recording daily. Someday maybe they will ask the people doing the work instead of assuming we are CP. Nah, can't happen to easy. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 16, 2014 4:06 PM To: 'Willis, Donna G.'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Histology Tracking systems Donna, we have touch screens, but our application is not fully touch screen friendly yet, so we have mini-keyboards that include a trackpad. They work well for login, choosing some items on the screen etc. They are not really meant for extensive use. 99% of the interaction is achieved simply by scanning a block or slide and using the touch screen as much as possible. the keyboard is just for that one percent in which the screen does not work so well. We do all QA recording on paper at the microtome and embedding center, and then computer entry later. Again, the system is not set up well for that aspect yet. If you are thinking of entering barcodes manually (you can do that if you know the ID) , yes, you can do it, it just slows things down a lot. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, October 16, 2014 2:00 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Histology Tracking systems I know this might be a strange question but, do any Histology Labs out in Histoland use a keyboard and mouse at Embedding and Microtomy to perform tracking, slide label printing and Quality Assurance recording. Thanks in advance for your replies. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 16 16:20:23 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Oct 16 16:20:44 2014 Subject: [Histonet] RE: Histology Tracking systems In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA320125284A07@Mail2Node2.ad.uams.edu> References: <2572B4D63B62E64A8078D8BBE34D407801BB5BAE@BHDASVEXML2.bhcs.pvt> <761E2B5697F795489C8710BCC72141FF367B0144@ex07.net.ucsf.edu> <41D3A1AF6FEF0643BDC89E0516A6EA320125284A07@Mail2Node2.ad.uams.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF367B0179@ex07.net.ucsf.edu> I'll add that our "QA" is just filling out exception forms if there is a problem with the block, not listing all of the blocks, slides, etc. Our system is generally good and a new version in the spring will have full touch screen capability - ie, designed for use with touch screens with large text and buttons (Cerner Copath Plus AB&T , v2013 now, v2014 in April). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: Marcum, Pamela A [mailto:PAMarcum@uams.edu] Sent: Thursday, October 16, 2014 2:17 PM To: Morken, Timothy; 'Willis, Donna G.'; Histonet (histonet@lists.utsouthwestern.edu) Subject: RE: Histology Tracking systems We do about the same thing with the embedding and sectioning. The computers are touch screen and some people use them, others use the pad on the keyboard, the rest asked for mice. We have no way to get the information we need for sectioning from the system so, we have to do the paperwork by hand. The system we have was created by engineers who thought we should do processing, embedding and staining, then deliver the slides. They missed sectioning completely and say they can't add it now. If you are on check out you get credit for sectioning, staining and delivering every slide cut that day. Just doesn't work well for workload recording daily. Someday maybe they will ask the people doing the work instead of assuming we are CP. Nah, can't happen to easy. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 16, 2014 4:06 PM To: 'Willis, Donna G.'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Histology Tracking systems Donna, we have touch screens, but our application is not fully touch screen friendly yet, so we have mini-keyboards that include a trackpad. They work well for login, choosing some items on the screen etc. They are not really meant for extensive use. 99% of the interaction is achieved simply by scanning a block or slide and using the touch screen as much as possible. the keyboard is just for that one percent in which the screen does not work so well. We do all QA recording on paper at the microtome and embedding center, and then computer entry later. Again, the system is not set up well for that aspect yet. If you are thinking of entering barcodes manually (you can do that if you know the ID) , yes, you can do it, it just slows things down a lot. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, October 16, 2014 2:00 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Histology Tracking systems I know this might be a strange question but, do any Histology Labs out in Histoland use a keyboard and mouse at Embedding and Microtomy to perform tracking, slide label printing and Quality Assurance recording. Thanks in advance for your replies. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From JMacDonald <@t> mtsac.edu Thu Oct 16 22:13:48 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Oct 16 22:13:59 2014 Subject: [Histonet] Storage of Glacial Acetic Acid In-Reply-To: <12ECD7346266D74691EC2BFC75285E4538117BF7@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E4538117BF7@BFL323E10.pathmdlabs.local> Message-ID: We store ours in the acid cabinet. It has a freezing point of 16.6 degrees Celsius so it needs to be kept at room temperature. From: Laurie Colbert To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Date: 10/16/2014 12:11 PM Subject: [Histonet] Storage of Glacial Acetic Acid Sent by: histonet-bounces@lists.utsouthwestern.edu How do you store GAA? As I recall, it should not be stored with other acids in an acid cabinet. I'm think it should be stored with flammables?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhartologist <@t> gmail.com Fri Oct 17 03:54:57 2014 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Fri Oct 17 03:55:00 2014 Subject: [Histonet] Reticle Stain Message-ID: Anyone know how to troubleshoot this Retic issue? The stain result is not the usual continuous silver stain reticulin fibers. Instead the fibers are more like dashed lines and the fibers are not visible throughout the entire tissue, especially edges. Ventana benchmark machines being used with their reagents. Thought maybe it was a reagent lot issue so tried different lot as well as different machine and getting similar results. Could it be the liver control we're using? Haven't had any issues with our tissue used as controls in the past for this stain. Any help on this will be appreciated! Thanks! From b-frederick <@t> northwestern.edu Fri Oct 17 07:21:45 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Oct 17 07:21:53 2014 Subject: [Histonet] RE: Histology Tracking systems In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA320125284A07@Mail2Node2.ad.uams.edu> References: <2572B4D63B62E64A8078D8BBE34D407801BB5BAE@BHDASVEXML2.bhcs.pvt> <761E2B5697F795489C8710BCC72141FF367B0144@ex07.net.ucsf.edu> <41D3A1AF6FEF0643BDC89E0516A6EA320125284A07@Mail2Node2.ad.uams.edu> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F112B02@evcspmbx2.ads.northwestern.edu> Our tracking system has everything there. We can create templates for various scenarios based on what is needed. The barcodes are generated by the system. It's a complicated system and takes a little time for data entry and such, but it does work. Our microscopy area is not set up in it as of yet, but it takes time. Once the requisition has been made we go in and make as completed for each task we perform. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Thursday, October 16, 2014 4:17 PM To: Morken, Timothy; 'Willis, Donna G.'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Histology Tracking systems We do about the same thing with the embedding and sectioning. The computers are touch screen and some people use them, others use the pad on the keyboard, the rest asked for mice. We have no way to get the information we need for sectioning from the system so, we have to do the paperwork by hand. The system we have was created by engineers who thought we should do processing, embedding and staining, then deliver the slides. They missed sectioning completely and say they can't add it now. If you are on check out you get credit for sectioning, staining and delivering every slide cut that day. Just doesn't work well for workload recording daily. Someday maybe they will ask the people doing the work instead of assuming we are CP. Nah, can't happen to easy. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, October 16, 2014 4:06 PM To: 'Willis, Donna G.'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Histology Tracking systems Donna, we have touch screens, but our application is not fully touch screen friendly yet, so we have mini-keyboards that include a trackpad. They work well for login, choosing some items on the screen etc. They are not really meant for extensive use. 99% of the interaction is achieved simply by scanning a block or slide and using the touch screen as much as possible. the keyboard is just for that one percent in which the screen does not work so well. We do all QA recording on paper at the microtome and embedding center, and then computer entry later. Again, the system is not set up well for that aspect yet. If you are thinking of entering barcodes manually (you can do that if you know the ID) , yes, you can do it, it just slows things down a lot. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, October 16, 2014 2:00 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Histology Tracking systems I know this might be a strange question but, do any Histology Labs out in Histoland use a keyboard and mouse at Embedding and Microtomy to perform tracking, slide label printing and Quality Assurance recording. Thanks in advance for your replies. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a_schade <@t> conradweiser.org Fri Oct 17 12:09:09 2014 From: a_schade <@t> conradweiser.org (Schade, Adelle) Date: Fri Oct 17 12:09:14 2014 Subject: [Histonet] processing and staining preserved specimen Message-ID: Hello everyone, I have been following and learning from this listserv for the past two years, I have enjoyed all of the advice this forum presents. This is my first inquiry for the group. I am a high school Anatomy and Physiology teacher and a grad student at Thomas Jefferson (Philadelphia, PA). In our research lab, we process, section, embed and image for all research projects. I was lucky enough to acquire some used histology equipment for my high school lab this school year. I am teaching the students about histology and the processing. We have an embedding station, microtome, staining dishes and a good microscope to image. We do not have an automatic tissue processor so we are processing manually (I prepare all solutions for the students). We are taking biopsy samples from our preserved mink that we are dissecting at the moment. I have processed the tissue using the following method and have pretty good results, not great. (we are doing a basic H&E stain to see the cells of the tissue). If we do not complete the manual processing method and need to leave school for the day, I move the cassettes into 70% EtOH until the next morning when we continue the process. I am wondering if anyone has manually processed preserved specimen tissue in the past and has any advice/ protocol that you would suggest? This is what I am using: Processing Method 1. 70% EtOH: 1 hour 2. 80% EtOH: 1 hour 3. 90% EtOH: 1 hour 4. 95% EtOH: 2 hours 5. 100% EtOH: 2 hours 6. Histoclear II: 2 hours 7. Paraffin: 2 hours Also- I will eventually be looking for grants to try to purchase an automatic tissue processor- this would alleviate the time issues we are facing. Does anyone know of grants that would focus on the educational aspect of histology? Thank you for your time and have a great weekend! Adelle Schade Graduate student: Jefferson School of Biomedical Science Ms. Adelle L. Schade, B.S., M.Ed. Anatomy and Physiology Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA 19551 a_schade@conradweiser.org From PAMarcum <@t> uams.edu Fri Oct 17 12:27:10 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Oct 17 12:27:22 2014 Subject: [Histonet] RE: processing and staining preserved specimen In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA320125287B41@Mail2Node2.ad.uams.edu> First of all THANK YOU for getting our field into a high school and teaching the kids. This is a very good thing for all of us in the field. Could you give us an idea of the size of the specimen you are attempting to process? A great deal depends on the tissue size as to how long it can be left in each reagent and paraffin. I realize your limitation in time and did hand processing of monkey and rat brains as well as other tissue, for years. No automated system was available and in research no money for one at the time. I would be more than happy to share. Have you checked with TJ and maybe HUP to see if they old equipment that is still good from a research lab that might be available to be donated? (Yes, I am from Philly) Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schade, Adelle Sent: Friday, October 17, 2014 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing and staining preserved specimen Hello everyone, I have been following and learning from this listserv for the past two years, I have enjoyed all of the advice this forum presents. This is my first inquiry for the group. I am a high school Anatomy and Physiology teacher and a grad student at Thomas Jefferson (Philadelphia, PA). In our research lab, we process, section, embed and image for all research projects. I was lucky enough to acquire some used histology equipment for my high school lab this school year. I am teaching the students about histology and the processing. We have an embedding station, microtome, staining dishes and a good microscope to image. We do not have an automatic tissue processor so we are processing manually (I prepare all solutions for the students). We are taking biopsy samples from our preserved mink that we are dissecting at the moment. I have processed the tissue using the following method and have pretty good results, not great. (we are doing a basic H&E stain to see the cells of the tissue). If we do not complete the manual processing method and need to leave school for the day, I move the cassettes into 70% EtOH until the next morning when we continue the process. I am wondering if anyone has manually processed preserved specimen tissue in the past and has any advice/ protocol that you would suggest? This is what I am using: Processing Method 1. 70% EtOH: 1 hour 2. 80% EtOH: 1 hour 3. 90% EtOH: 1 hour 4. 95% EtOH: 2 hours 5. 100% EtOH: 2 hours 6. Histoclear II: 2 hours 7. Paraffin: 2 hours Also- I will eventually be looking for grants to try to purchase an automatic tissue processor- this would alleviate the time issues we are facing. Does anyone know of grants that would focus on the educational aspect of histology? Thank you for your time and have a great weekend! Adelle Schade Graduate student: Jefferson School of Biomedical Science Ms. Adelle L. Schade, B.S., M.Ed. Anatomy and Physiology Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA 19551 a_schade@conradweiser.org ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ttruscot <@t> vetmed.wsu.edu Fri Oct 17 12:31:57 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Oct 17 12:32:10 2014 Subject: [Histonet] RE: processing and staining preserved specimen In-Reply-To: References: Message-ID: <9EF5279EBDFE6E4FB6605E8F183A0027C87E84E4@CVM76.vetmed.wsu.edu> Hi Adelle, Biopsy sizes will matter, but you might shorten some of these times, but be sure to add at least one more Histoclear to make sure there is no alcohol in it and at least one more paraffin to make sure there is no histoclear carryover.If you go back to 70% at any time you will have to start over, so try to get a janitor or someone to start early or a coach to finish late. Make sure you keep things safe, because of all the flammables. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schade, Adelle Sent: Friday, October 17, 2014 10:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing and staining preserved specimen Hello everyone, I have been following and learning from this listserv for the past two years, I have enjoyed all of the advice this forum presents. This is my first inquiry for the group. I am a high school Anatomy and Physiology teacher and a grad student at Thomas Jefferson (Philadelphia, PA). In our research lab, we process, section, embed and image for all research projects. I was lucky enough to acquire some used histology equipment for my high school lab this school year. I am teaching the students about histology and the processing. We have an embedding station, microtome, staining dishes and a good microscope to image. We do not have an automatic tissue processor so we are processing manually (I prepare all solutions for the students). We are taking biopsy samples from our preserved mink that we are dissecting at the moment. I have processed the tissue using the following method and have pretty good results, not great. (we are doing a basic H&E stain to see the cells of the tissue). If we do not complete the manual processing method and need to leave school for the day, I move the cassettes into 70% EtOH until the next morning when we continue the process. I am wondering if anyone has manually processed preserved specimen tissue in the past and has any advice/ protocol that you would suggest? This is what I am using: Processing Method 1. 70% EtOH: 1 hour 2. 80% EtOH: 1 hour 3. 90% EtOH: 1 hour 4. 95% EtOH: 2 hours 5. 100% EtOH: 2 hours 6. Histoclear II: 2 hours 7. Paraffin: 2 hours Also- I will eventually be looking for grants to try to purchase an automatic tissue processor- this would alleviate the time issues we are facing. Does anyone know of grants that would focus on the educational aspect of histology? Thank you for your time and have a great weekend! Adelle Schade Graduate student: Jefferson School of Biomedical Science Ms. Adelle L. Schade, B.S., M.Ed. Anatomy and Physiology Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA 19551 a_schade@conradweiser.org From a_schade <@t> conradweiser.org Fri Oct 17 13:08:28 2014 From: a_schade <@t> conradweiser.org (Schade, Adelle) Date: Fri Oct 17 13:08:34 2014 Subject: [Histonet] RE: processing and staining preserved specimen In-Reply-To: <9EF5279EBDFE6E4FB6605E8F183A0027C87E84E4@CVM76.vetmed.wsu.edu> References: <9EF5279EBDFE6E4FB6605E8F183A0027C87E84E4@CVM76.vetmed.wsu.edu> Message-ID: Thank you Tom and Pat- Here are the biopsy sizes: I have my classes experimenting with specimen size to try to find what will work best for our situation. My 1st block class has 2mm biopsy cores, 2nd block has 4mm biopsy cores and 3rd block has 6mm biopsy cores- each about 2- 3 mm thick. I will try some specimen altering the times as Tom suggested to see what we get- Thanks again Adelle -----Original Message----- From: Truscott, Tom [mailto:ttruscot@vetmed.wsu.edu] Sent: Friday, October 17, 2014 1:32 PM To: Schade, Adelle; histonet@lists.utsouthwestern.edu Subject: RE: processing and staining preserved specimen Hi Adelle, Biopsy sizes will matter, but you might shorten some of these times, but be sure to add at least one more Histoclear to make sure there is no alcohol in it and at least one more paraffin to make sure there is no histoclear carryover.If you go back to 70% at any time you will have to start over, so try to get a janitor or someone to start early or a coach to finish late. Make sure you keep things safe, because of all the flammables. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schade, Adelle Sent: Friday, October 17, 2014 10:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing and staining preserved specimen Hello everyone, I have been following and learning from this listserv for the past two years, I have enjoyed all of the advice this forum presents. This is my first inquiry for the group. I am a high school Anatomy and Physiology teacher and a grad student at Thomas Jefferson (Philadelphia, PA). In our research lab, we process, section, embed and image for all research projects. I was lucky enough to acquire some used histology equipment for my high school lab this school year. I am teaching the students about histology and the processing. We have an embedding station, microtome, staining dishes and a good microscope to image. We do not have an automatic tissue processor so we are processing manually (I prepare all solutions for the students). We are taking biopsy samples from our preserved mink that we are dissecting at the moment. I have processed the tissue using the following method and have pretty good results, not great. (we are doing a basic H&E stain to see the cells of the tissue). If we do not complete the manual processing method and need to leave school for the day, I move the cassettes into 70% EtOH until the next morning when we continue the process. I am wondering if anyone has manually processed preserved specimen tissue in the past and has any advice/ protocol that you would suggest? This is what I am using: Processing Method 1. 70% EtOH: 1 hour 2. 80% EtOH: 1 hour 3. 90% EtOH: 1 hour 4. 95% EtOH: 2 hours 5. 100% EtOH: 2 hours 6. Histoclear II: 2 hours 7. Paraffin: 2 hours Also- I will eventually be looking for grants to try to purchase an automatic tissue processor- this would alleviate the time issues we are facing. Does anyone know of grants that would focus on the educational aspect of histology? Thank you for your time and have a great weekend! Adelle Schade Graduate student: Jefferson School of Biomedical Science Ms. Adelle L. Schade, B.S., M.Ed. Anatomy and Physiology Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA 19551 a_schade@conradweiser.org From joelleweaver <@t> hotmail.com Fri Oct 17 13:59:55 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Oct 17 14:00:03 2014 Subject: [Histonet] Histology Tracking systems In-Reply-To: <2572B4D63B62E64A8078D8BBE34D407801BB5BAE@BHDASVEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D407801BB5BAE@BHDASVEXML2.bhcs.pvt> Message-ID: I have used a keyboard at embedding, but no mouse. The other tracking uses I have seen use scanning of bar codes or QR codes not keyboard or mouse entry. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Donna.Willis@baylorhealth.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 16 Oct 2014 21:00:27 +0000 > Subject: [Histonet] Histology Tracking systems > > I know this might be a strange question but, do any Histology Labs out in Histoland use a keyboard and mouse at Embedding and Microtomy to perform tracking, slide label printing and Quality Assurance recording. Thanks in advance for your replies. > > Donna Willis, HT/HTL(ASCP) > Anatomic Pathology Manager > Baylor University Medical Center > 3500 Gaston Ave|Dallas, Texas 75246 > 214-820-2465 office|214-725-6184 mobile > BaylorScottandWhite.com > > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From avistarop <@t> ffyb.uba.ar Fri Oct 17 14:30:44 2014 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Fri Oct 17 14:31:01 2014 Subject: [Histonet] reduce Paraffin fluorescence Message-ID: <2c1242e6ff199d9876474dc353bc1cb7.squirrel@huemul.ffyb.uba.ar> Hello!!!! I am writing to request if you could send me the protocol of Inmunohistochemical staining for reduce the Paraffin fluorescence. I saw that the people use evans blue, but I don?t know how can I do? Thank you Good weekend!!!! ALDANA VISTAROP Laboratorio Biologi?a Molecular, Servicio de Anatomia Patologica, Hospital de Ni?os Ricardo Gutierrez. From Albert.Santiago <@t> uphs.upenn.edu Fri Oct 17 15:21:52 2014 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Fri Oct 17 15:22:04 2014 Subject: [Histonet] FW: Formalin use in grossing In-Reply-To: References: Message-ID: Hello again my fellow histonetters, I was hoping someone could give me their thoughts on this issue: We receive Derm biopsy specimens in formalin containers. We gross the specimen and place the cassettes with the tissue in it in a tray with 10% formalin. Although we have grossing tables with exhaust vents, I would like to minimize the formalin odors even more if possible. Therefore, I was thinking about replacing the 10% formalin in the buckets with either graded alcohol (50% or 70%) or water in the buckets before putting the cassettes in the processor. I'm wondering if that would negatively affect the tissue, H&E staining or IHC staining. Also, if I can make the change would I still be compliant with The Joint Commission and/or CAP? As always, thank you for your feedback. Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago@uphs.upenn.edu From mikael.niku <@t> helsinki.fi Mon Oct 20 06:00:11 2014 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Mon Oct 20 06:00:37 2014 Subject: [Histonet] Staining bacteria in cryo + paraffin In-Reply-To: <1411406916.22931.YahooMailNeo@web180906.mail.ne1.yahoo.com> References: <1411406916.22931.YahooMailNeo@web180906.mail.ne1.yahoo.com> Message-ID: <5444EB3B.5050909@helsinki.fi> Hello! What is your preferred method to show ANY bacteria (mostly intestinal flora) on cryo AND paraffin sections? Two different stains OK. With best regards, Mikael From MICHELLE.LAMPHERE <@t> childrens.com Mon Oct 20 08:08:35 2014 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Mon Oct 20 08:09:35 2014 Subject: [Histonet] Dallas job openings Message-ID: We have full and part time openings at Children's Health in Dallas, TX. Positions require qualification for High Complexity Testing. Please contact me directly to inquire. Michelle Lamphere Senior Tech, Histology Children's Health 214-456-2318 michelle.lamphere@childrens.com Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From freidac <@t> sbcglobal.net Mon Oct 20 08:43:00 2014 From: freidac <@t> sbcglobal.net (Freida Carson) Date: Mon Oct 20 08:43:09 2014 Subject: [Histonet] Protargol S Message-ID: <9C53495D-F306-43AE-B345-B07885529CA2@sbcglobal.net> Does anyone have a source for good protargol for Bodian stains? A friend in Sweden is asking. Sent from my iPhone From tina.vanmeter <@t> gmail.com Mon Oct 20 09:02:45 2014 From: tina.vanmeter <@t> gmail.com (Tina Van Meter) Date: Mon Oct 20 09:56:10 2014 Subject: [Histonet] Protargol S In-Reply-To: <9C53495D-F306-43AE-B345-B07885529CA2@sbcglobal.net> References: <9C53495D-F306-43AE-B345-B07885529CA2@sbcglobal.net> Message-ID: ?Hi Freida, American MasterTech has a Bodian Stain Kit #KC245Z, $48.00 Kind regards, Tina Tina Van Meter, HT (ASCP) Histology Core Manager Scripps Research Institute Jupiter, FL On Mon, Oct 20, 2014 at 9:43 AM, Freida Carson wrote: > Does anyone have a source for good protargol for Bodian stains? A friend > in Sweden is asking. > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rebecca.Riesen <@t> hma.com Mon Oct 20 09:57:36 2014 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Mon Oct 20 09:58:18 2014 Subject: [Histonet] RE: Histonet Digest, Vol 131, Issue 20 In-Reply-To: <9a7e7a0b-abd2-4de0-96ab-2a512c11df12@TN001WEXCHCAS07.US.chs.net> References: <9a7e7a0b-abd2-4de0-96ab-2a512c11df12@TN001WEXCHCAS07.US.chs.net> Message-ID: We had this issue and found that there was contamination in the lines and buffer of the Benchmark. After working with Ventana it was noticed that "big" labs that went through their kits quickly were not experiencing the problem, but us "little-guys" had an issue after a certain period or time (which was determined by how quickly each lab would exhaust a kit) We ended up with a Ventana tech having to come do extra decontaminations on our instrument almost every other week followed by preparing new buffer solution. It only seemed to happen on the Silver Stains. I believe Ventana is working on a solution to the problem, but I'm unsure of where they are in the process. Sorry, I don't have a fix, other than extra decontaminations. ------------------------------ Message: 14 Date: Fri, 17 Oct 2014 01:54:57 -0700 From: Bharti Parihar Subject: [Histonet] Reticle Stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Anyone know how to troubleshoot this Retic issue? The stain result is not the usual continuous silver stain reticulin fibers. Instead the fibers are more like dashed lines and the fibers are not visible throughout the entire tissue, especially edges. Ventana benchmark machines being used with their reagents. Thought maybe it was a reagent lot issue so tried different lot as well as different machine and getting similar results. Could it be the liver control we're using? Haven't had any issues with our tissue used as controls in the past for this stain. Any help on this will be appreciated! Thanks! -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From eca9 <@t> georgetown.edu Mon Oct 20 10:13:40 2014 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Mon Oct 20 10:14:18 2014 Subject: [Histonet] Mount-Quick for tissue transfer? Message-ID: Good morning Histonetters, I was wondering if anyone has used Mount-Quick to transfer tissue from uncharged slides to charged slides. We had a submitter give us slides for staining on uncharged slides and the tissue comes off the slide during antigen retrieval. I remember reading about this product but have never used it. Is it hard to do? Are the slides staining well afterwards? Thank you, Eva Georgetown University From rjbuesa <@t> yahoo.com Mon Oct 20 10:29:08 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Oct 20 10:29:13 2014 Subject: [Histonet] Protargol S In-Reply-To: <9C53495D-F306-43AE-B345-B07885529CA2@sbcglobal.net> References: <9C53495D-F306-43AE-B345-B07885529CA2@sbcglobal.net> Message-ID: <1649348788.286357.1413818948621.JavaMail.yahoo@jws100187.mail.ne1.yahoo.com> Protargol, as you well know, oxidizes very easily after you open the container, so I?prepared my own protargol every time I needed it and the results were always consistent and of great quality.Under separate cover I am forwarding you?an article?I wrote on the JoH on the subject.Ren? J.? On Monday, October 20, 2014 9:43 AM, Freida Carson wrote: Does anyone have a source for good protargol for Bodian stains? A friend in Sweden is asking. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tejohnson <@t> genoptix.com Mon Oct 20 12:04:47 2014 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Mon Oct 20 12:05:13 2014 Subject: [Histonet] Re: processing and staining preserved specimen Message-ID: With the 100% ETOH, Histoclear, and paraffin, you will need two changes as has already been mentioned. Dense, thick or fatty tissues require more time. Given these are small animal tissues you may be able to cut your time in each station in half. You also may think about using isopropanol rather than the more expensive ethanol. So, add a change of each as above and try processing in each one 30 minutes and see how that works for you. This makes this about a 5 hour process which may be more doable. However, you may find that without pressure/vacuum in the paraffin you may need one hour in each change for good infiltration. Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From TanyaAbbott <@t> catholichealth.net Mon Oct 20 12:28:31 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Oct 20 12:28:47 2014 Subject: [Histonet] RE: Histonet Digest, Vol 131, Issue 22 ] Reticle Stain In-Reply-To: <201410201702.s9KGwlB2004869@chippi003.catholichealth.net> References: <201410201702.s9KGwlB2004869@chippi003.catholichealth.net> Message-ID: <852F7D2C14FB464D80E182B15DB138AF394ECAD1@CHIEX005.CHI.catholichealth.net> I agree with Rebecca. We had this problems constantly at the previous hospital I worked at and were in contact with Ventana frequently regarding this issue. It is my understanding that this hospital is still having problems and that a Ventana rep is in there frequently to perform decons. It's all about the decons! Very frustrating! Where I am now, we hand stain our retics, no problems!! Good luck! Tanya G. Abbott Histology/Cytology St. Joseph Medical Center 610-378-2635 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, October 20, 2014 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 131, Issue 22 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=aoYBCDzxYnCqyU7xQM4r6zHUccHtUfqhF05yOF%2FCYNc%3D%0A&m=MKOSm08FchqE2lZwrQir%2Ff4nTzJfLtcd5ceuUwyykWI%3D%0A&s=6970511c6179dc771f91e69f5d3d2a2a5a4fa41d5914f3bad84f2232ddf693ef or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Staining bacteria in cryo + paraffin (Mikael Niku) 2. Dallas job openings (Michelle Lamphere) 3. Protargol S (Freida Carson) 4. Re: Protargol S (Tina Van Meter) 5. RE: Histonet Digest, Vol 131, Issue 20 (Riesen, Rebecca) 6. Mount-Quick for tissue transfer? (Eva Permaul) 7. Re: Protargol S (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 20 Oct 2014 14:00:11 +0300 From: Mikael Niku Subject: [Histonet] Staining bacteria in cryo + paraffin To: histonet@lists.utsouthwestern.edu Message-ID: <5444EB3B.5050909@helsinki.fi> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello! What is your preferred method to show ANY bacteria (mostly intestinal flora) on cryo AND paraffin sections? Two different stains OK. With best regards, Mikael ------------------------------ Message: 2 Date: Mon, 20 Oct 2014 13:08:35 +0000 From: Michelle Lamphere Subject: [Histonet] Dallas job openings To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" We have full and part time openings at Children's Health in Dallas, TX. Positions require qualification for High Complexity Testing. Please contact me directly to inquire. Michelle Lamphere Senior Tech, Histology Children's Health 214-456-2318 michelle.lamphere@childrens.com Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ------------------------------ Message: 3 Date: Mon, 20 Oct 2014 08:43:00 -0500 From: Freida Carson Subject: [Histonet] Protargol S To: Histonet Message-ID: <9C53495D-F306-43AE-B345-B07885529CA2@sbcglobal.net> Content-Type: text/plain; charset=us-ascii Does anyone have a source for good protargol for Bodian stains? A friend in Sweden is asking. Sent from my iPhone ------------------------------ Message: 4 Date: Mon, 20 Oct 2014 10:02:45 -0400 From: Tina Van Meter Subject: Re: [Histonet] Protargol S To: Freida Carson Cc: Histonet Message-ID: Content-Type: text/plain; charset=UTF-8 ???Hi Freida, American MasterTech has a Bodian Stain Kit #KC245Z, $48.00 Kind regards, Tina Tina Van Meter, HT (ASCP) Histology Core Manager Scripps Research Institute Jupiter, FL On Mon, Oct 20, 2014 at 9:43 AM, Freida Carson wrote: > Does anyone have a source for good protargol for Bodian stains? A > friend in Sweden is asking. > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern > .edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=aoY > BCDzxYnCqyU7xQM4r6zHUccHtUfqhF05yOF%2FCYNc%3D%0A&m=MKOSm08FchqE2lZwrQi > r%2Ff4nTzJfLtcd5ceuUwyykWI%3D%0A&s=6970511c6179dc771f91e69f5d3d2a2a5a4 > fa41d5914f3bad84f2232ddf693ef > ------------------------------ Message: 5 Date: Mon, 20 Oct 2014 14:57:36 +0000 From: "Riesen, Rebecca" Subject: [Histonet] RE: Histonet Digest, Vol 131, Issue 20 To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We had this issue and found that there was contamination in the lines and buffer of the Benchmark. After working with Ventana it was noticed that "big" labs that went through their kits quickly were not experiencing the problem, but us "little-guys" had an issue after a certain period or time (which was determined by how quickly each lab would exhaust a kit) We ended up with a Ventana tech having to come do extra decontaminations on our instrument almost every other week followed by preparing new buffer solution. It only seemed to happen on the Silver Stains. I believe Ventana is working on a solution to the problem, but I'm unsure of where they are in the process. Sorry, I don't have a fix, other than extra decontaminations. ------------------------------ Message: 14 Date: Fri, 17 Oct 2014 01:54:57 -0700 From: Bharti Parihar Subject: [Histonet] Reticle Stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Anyone know how to troubleshoot this Retic issue? The stain result is not the usual continuous silver stain reticulin fibers. Instead the fibers are more like dashed lines and the fibers are not visible throughout the entire tissue, especially edges. Ventana benchmark machines being used with their reagents. Thought maybe it was a reagent lot issue so tried different lot as well as different machine and getting similar results. Could it be the liver control we're using? Haven't had any issues with our tissue used as controls in the past for this stain. Any help on this will be appreciated! Thanks! -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 6 Date: Mon, 20 Oct 2014 11:13:40 -0400 From: Eva Permaul Subject: [Histonet] Mount-Quick for tissue transfer? To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Good morning Histonetters, I was wondering if anyone has used Mount-Quick to transfer tissue from uncharged slides to charged slides. We had a submitter give us slides for staining on uncharged slides and the tissue comes off the slide during antigen retrieval. I remember reading about this product but have never used it. Is it hard to do? Are the slides staining well afterwards? Thank you, Eva Georgetown University ------------------------------ Message: 7 Date: Mon, 20 Oct 2014 15:29:08 +0000 (UTC) From: Rene J Buesa Subject: Re: [Histonet] Protargol S To: Freida Carson , Histonet Message-ID: <1649348788.286357.1413818948621.JavaMail.yahoo@jws100187.mail.ne1.yahoo.com> Content-Type: text/plain; charset=UTF-8 Protargol, as you well know, oxidizes very easily after you open the container, so I??prepared my own protargol every time I needed it and the results were always consistent and of great quality.Under separate cover I am forwarding you??an article??I wrote on the JoH on the subject.Ren?? J.?? On Monday, October 20, 2014 9:43 AM, Freida Carson wrote: Does anyone have a source for good protargol for Bodian stains? A friend in Sweden is asking. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=aoYBCDzxYnCqyU7xQM4r6zHUccHtUfqhF05yOF%2FCYNc%3D%0A&m=MKOSm08FchqE2lZwrQir%2Ff4nTzJfLtcd5ceuUwyykWI%3D%0A&s=6970511c6179dc771f91e69f5d3d2a2a5a4fa41d5914f3bad84f2232ddf693ef ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=aoYBCDzxYnCqyU7xQM4r6zHUccHtUfqhF05yOF%2FCYNc%3D%0A&m=MKOSm08FchqE2lZwrQir%2Ff4nTzJfLtcd5ceuUwyykWI%3D%0A&s=6970511c6179dc771f91e69f5d3d2a2a5a4fa41d5914f3bad84f2232ddf693ef End of Histonet Digest, Vol 131, Issue 22 ***************************************** This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From AJohnson <@t> aipathology.com Mon Oct 20 12:39:35 2014 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Mon Oct 20 12:39:49 2014 Subject: [Histonet] QIHC exam Message-ID: Is there any info or mock exams on the QIHC that people can get to see if they are ready to take the exam?? Thanks, Amylin Johnson, BS HTL(ASCP) Associates in Pathology Wausau, WI 715-847-2130 From Stacy_McLaughlin <@t> cooley-dickinson.org Mon Oct 20 13:10:29 2014 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Mon Oct 20 13:10:42 2014 Subject: [Histonet] Open Position HT/HTL Message-ID: The Cooley Dickinson Hospital in Northampton, MA has an opening for a 30 hour per week HT/HTL. We are a community hospital with ~120 beds and we process just under 10,000 surgical cases a year. The Histology laboratory team consists of one 40 hour HT, a working supervisor, a part time lab assistant, and 3 secretaries. We have 3 pathologists that are great to work with! We use VIP tissue processors, Leica microtomes, stainers, and coverslipper, and Ventana instrumentation for IHC. Hours of the position are 5am-11am Monday-Friday. If you're interested, please message me off line. Thank you, Stacy Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton,MA 01060 (413) 582-2019 Stacy_McLaughlin@Cooley-Dickinson.org [Cooley-Dickinson.org] From bethcoxx <@t> gmail.com Tue Oct 21 12:12:04 2014 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Tue Oct 21 12:12:10 2014 Subject: [Histonet] Re: QIHC exam In-Reply-To: <5446912e.c4b7ca0a.4d00.ffffaf93SMTPIN_ADDED_MISSING@mx.google.com> References: <5446912e.c4b7ca0a.4d00.ffffaf93SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <544693E4.90207@gmail.com> Amylin, The Michigan Society of Histotechnologists has Study Workbook for the QIHC exam. It was written to cover all the things you need to study for the exam (and avoids the things not covered on the exam). To order, go to mihisto.org cost:$20 Beth Cox, HTL/SCT(ASCP)QIHC Pathology Manager Beebe Healthcare Lewes, De Message: 3 Date: Mon, 20 Oct 2014 17:39:35 +0000 From: Amy Johnson Subject: [Histonet] QIHC exam To:"'histonet@lists.utsouthwestern.edu'" , "'histonet-bounces@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Is there any info or mock exams on the QIHC that people can get to see if they are ready to take the exam?? Thanks, Amylin Johnson, BS HTL(ASCP) Associates in Pathology Wausau, WI 715-847-2130 From jaylundgren <@t> gmail.com Tue Oct 21 12:24:26 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Oct 21 12:24:32 2014 Subject: [Histonet] FW: Formalin use in grossing In-Reply-To: References: Message-ID: Have you tried improvising a cover for the cassette bucket? It cuts down on fumes considerably. You only get one chance to properly fix something, so I always lean in the direction of more fixation, not less (within reason, I know about the guidelines for HER2). Or, get rid of formalin in your lab if it bothers you that much. There are acceptable substitutes out there. You can supply the fixative to your derm clinicians. Problem totally solved. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Oct 17, 2014 at 3:21 PM, Santiago, Albert < Albert.Santiago@uphs.upenn.edu> wrote: > > Hello again my fellow histonetters, I was hoping someone could give me > their thoughts on this issue: > We receive Derm biopsy specimens in formalin containers. We gross the > specimen and place the cassettes with the tissue in it in a tray with 10% > formalin. Although we have grossing tables with exhaust vents, I would > like to minimize the formalin odors even more if possible. Therefore, I was > thinking about replacing the 10% formalin in the buckets with either graded > alcohol (50% or 70%) or water in the buckets before putting the cassettes > in the processor. > I'm wondering if that would negatively affect the tissue, H&E staining or > IHC staining. > Also, if I can make the change would I still be compliant with The Joint > Commission and/or CAP? > > As always, thank you for your feedback. > > > Albert Santiago, HT(ASCP) > Lab Manager > Penncutaneous Pathology Services > Dermatopathology Lab > 3020 Market ST. Ste 201 > Philadelphia, PA 19104 > 215-662-6539 - Lab > 215-662-6759-office > albert.santiago@uphs.upenn.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amber.mckenzie <@t> gastrodocs.net Tue Oct 21 12:27:43 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Oct 21 12:28:40 2014 Subject: [Histonet] Re: QIHC exam In-Reply-To: <544693E4.90207@gmail.com> References: <5446912e.c4b7ca0a.4d00.ffffaf93SMTPIN_ADDED_MISSING@mx.google.com> <544693E4.90207@gmail.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0112559868@JERRY.Gia.com> BUT, there is no answer key! I don't like that. I wish they were in the back of the book to review. Amber McKenzie | Histology Supervisor GI Associates & Endoscopy Center 1405 North State Street, Suite 400 Jackson, MS 39202 Phone | 601-863-0381 Fax | 601-326-3532 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Tuesday, October 21, 2014 12:12 PM To: histonet@lists.utsouthwestern.edu; AJohnson@aipathology.com Subject: [Histonet] Re: QIHC exam Amylin, The Michigan Society of Histotechnologists has Study Workbook for the QIHC exam. It was written to cover all the things you need to study for the exam (and avoids the things not covered on the exam). To order, go to mihisto.org cost:$20 Beth Cox, HTL/SCT(ASCP)QIHC Pathology Manager Beebe Healthcare Lewes, De Message: 3 Date: Mon, 20 Oct 2014 17:39:35 +0000 From: Amy Johnson Subject: [Histonet] QIHC exam To:"'histonet@lists.utsouthwestern.edu'" , "'histonet-bounces@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Is there any info or mock exams on the QIHC that people can get to see if they are ready to take the exam?? Thanks, Amylin Johnson, BS HTL(ASCP) Associates in Pathology Wausau, WI 715-847-2130 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Prentice <@t> ucsf.edu Tue Oct 21 14:04:40 2014 From: Linda.Prentice <@t> ucsf.edu (Prentice, Linda) Date: Tue Oct 21 14:06:18 2014 Subject: [Histonet] drosophila paraffin histology technique Message-ID: I would appreciate help with finding a good paraffin protocol for drosophila (fruit flies). I am currently using Bouin's fixative followed by a routine alcohol, xylene substitute and paraffin infiltration technique. I need a protocol that will allow better infiltration than I am currently getting, I have tried potassium hydroxide to "soften" the outer layer of the fruit fly but there was only a slight improvement. We are looking at the Malpighian tubules and I will need serial section. thanks Linda Prentice University of California, SF Preventive and Restorative Dental Sciences. From kiran_g <@t> sbcglobal.net Tue Oct 21 17:28:48 2014 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Tue Oct 21 17:28:54 2014 Subject: [Histonet] Job Opening: Histology/IHC Manager Message-ID: <1413930528.26299.YahooMailNeo@web184801.mail.gq1.yahoo.com> Hi All, Great opportunity to work with Kaiser Permanente Regional Laboratory located in Berkeley, CA serving Northern California Kaiser Hospitals. Excellent benefits and state of the art lab with Ventana & Leica Staining platforms, Hours of the position are 8am-5pm M-F. If interested, please contact me, Thanks. Kiran Kiranjit Grewal Director of Lab. Services Anatomic Pathology. Kaiser Regional Lab. Berkeley, CA Kiranjit.K.Grewal@kp.org From o.m.gallagher <@t> sheffield.ac.uk Wed Oct 22 10:02:55 2014 From: o.m.gallagher <@t> sheffield.ac.uk (Orla M Gallagher) Date: Wed Oct 22 10:03:05 2014 Subject: [Histonet] Fixation in concentrated 37% formaldehyde Message-ID: Dear Histonetters, What would the effect be of fixing tissues samples in concentrated formaldehyde instead of 10% buffered formalin? One of our researchers would like us to prepare some bones for histology staining which have been fixed in 37% formaldehyde by mistake and stored for 4 years. I assume there will be formalin pigment in these samples, so even a H&E may not look great, while any enzyme histochemistry or immunohistochemistry is probably not worth trying. I haven't seen the samples yet as they will be arriving from another UK university. Has anyone out there fixed any tissues in concentrated formaldehyde by accident or by design? Thanks, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/visitors/mapsandtravel From rjbuesa <@t> yahoo.com Wed Oct 22 10:19:34 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 22 10:19:42 2014 Subject: [Histonet] Fixation in concentrated 37% formaldehyde In-Reply-To: References: Message-ID: <1965794130.517498.1413991174464.JavaMail.yahoo@jws10036.mail.ne1.yahoo.com> Using "pure" formaldehyde as a fixative is not a good idea (you already know that) and the staining will be affected BUT, on the other hand, IHC will only require stronger HIER (more time and temperature) but other than that I do think the epitopes will be detected. So the "effects" will be mostly "cosmetic".Ren? J.?? On Wednesday, October 22, 2014 11:04 AM, Orla M Gallagher wrote: Dear Histonetters, What would the effect be of fixing tissues samples in concentrated formaldehyde instead of 10% buffered formalin? One of our researchers would like us to prepare some bones for histology staining which have been fixed in 37% formaldehyde by mistake and stored for 4 years. I assume there will be formalin pigment in these samples, so even a H&E may not look great, while any enzyme histochemistry or immunohistochemistry is probably not worth trying. I haven't seen the samples yet as they will be arriving from another UK university. Has anyone out there fixed any tissues in concentrated formaldehyde by accident or by design? Thanks, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel:? ? ? ? 0044114-2713337 (office) ? ? ? ? ? ? ? 0044114-2713174 (lab) E-Mail:? ? o.m.gallagher@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. http://www.shef.ac.uk/faculty/medicine-dentistry-health/2.9274/emailwording http://www.sheffield.ac.uk/visitors/mapsandtravel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kristofer.Rodriguez <@t> bcm.edu Wed Oct 22 10:31:24 2014 From: Kristofer.Rodriguez <@t> bcm.edu (Rodriguez, Kristofer) Date: Wed Oct 22 10:33:48 2014 Subject: [Histonet] Antibody deposit visualization Message-ID: One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine H&E will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an H&E. I was wondering if I should do any special stains that might show more than an H&E. From rjbuesa <@t> yahoo.com Wed Oct 22 10:59:51 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Oct 22 10:59:57 2014 Subject: [Histonet] Antibody deposit visualization In-Reply-To: References: Message-ID: <1206387319.524730.1413993591041.JavaMail.yahoo@jws100167.mail.ne1.yahoo.com> Depending on the reaction of the glomerulii to the Dengue virus and the stage of such reaction perhaps a PAM (periodic acid methenamine silver) stain could show something.Ren? J.? On Wednesday, October 22, 2014 11:31 AM, "Rodriguez, Kristofer" wrote: One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine H&E will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an H&E. I was wondering if I should do any special stains that might show more than an H&E. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.Whitaker <@t> osumc.edu Wed Oct 22 12:06:36 2014 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Wed Oct 22 12:06:58 2014 Subject: [Histonet] ATRX Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E0BC51531@EXMBOX-VP05.OSUMC.EDU> Hi Everyone, Does anyone have ATRX available as a clinical test? I need some that will do the technical only for us. Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 From kalschev <@t> svm.vetmed.wisc.edu Wed Oct 22 13:32:19 2014 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Wed Oct 22 13:32:23 2014 Subject: [Histonet] Linda - drosophila Message-ID: <5447F833.5010706@svm.vetmed.wisc.edu> Have you tried any plastic methods? Feel free to contact me. -- Vicki L. Kalscheur School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 2015 Linden Drive Madison, WI 53706-1100 Phone: 608-262-8534 kalschev@vetmed.wisc.edu From j.benavides <@t> eae.csic.es Wed Oct 22 14:25:29 2014 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Wed Oct 22 14:25:37 2014 Subject: [Histonet] Antibody deposit visualization In-Reply-To: References: Message-ID: <20141022212529.Horde.h7wdP9_JM0ovAqevv3p3pA5@webmail.csic.es> Hi, may be you can try to label murine immunoglobulins. A mere secondary against murine IgGs could do the trick, depending on which isotype your client is suspecting to form the precipitates. Just an idea, never have tried before. Cheers Julio "Rodriguez, Kristofer" escribi?: > One of my clients has been performing experiments with a mouse model > of Dengue Virus Infection. They have been treating the mice with > monoclonal antibodies (client did not specify what antibody)as a > potential treatment for dengue virus infection. They have harvested > the kidneys and put them in 10% NBF. They want to know if routine > H&E will show any antibody deposits. > > I told him that I would not be able to directly observe the antibody > deposit itself, however I might be able to observe the reaction to > the antibody deposit in the basement membrane of the glomeruli as a > membranous glomerulopathy on an H&E. > > I was wondering if I should do any special stains that might show > more than an H&E. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Fauck <@t> ccdhb.org.nz Wed Oct 22 14:27:45 2014 From: Robert.Fauck <@t> ccdhb.org.nz (Robert Fauck [CCDHB]) Date: Wed Oct 22 14:27:54 2014 Subject: [Histonet] Unsubscribe me please Message-ID: Can you please unsubscribe me from your Histo-List. Thanks, Robert Fauck This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) From Joyce.Weems <@t> emoryhealthcare.org Wed Oct 22 14:52:47 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Oct 22 14:52:58 2014 Subject: [Histonet] RE: Unsubscribe me please In-Reply-To: References: Message-ID: You will need to do that at the website - http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB] Sent: Wednesday, October 22, 2014 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Unsubscribe me please Can you please unsubscribe me from your Histo-List. Thanks, Robert Fauck This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From abtdhu <@t> gmail.com Wed Oct 22 15:20:04 2014 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Wed Oct 22 15:20:07 2014 Subject: [Histonet] Protocol of MMA Plastic section TRAP staining Message-ID: Dear Histoneters, Can someone email me your TRAP protocol for MMA section? I have one, but staining is not consistent. No matter I stain for how long, ~ several hours sometimes. There are always variations. I sincerely hope I can get different protocol form you and make change. I remember saw other TRAP protocol which is much longer than mine. The protocol I used was: 1. Dissolve 12mg Naphthol AS-MX phosphate (Sigma, N-5000) in 0.5ml N,N methylformamide (Sigma,D8654). 2. Mix above into 50 ml Sodium tartrate with 0.1M sodium acetate buffer at pH5.0. 3. Mix 30mg Fast Red Violet LB salt (Sigma, F3381) with above solution and filter. Stain slides @37oC for at least one hour. The solution can be use for one month when stored in 4oC. Continue to do counterstain and mount slides. Thank you. Dorothy Hu MGH Endocrine histocore Email: dhu5@partners.org From liz <@t> premierlab.com Wed Oct 22 15:29:10 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Oct 22 15:29:14 2014 Subject: [Histonet] RE: Antibody deposit visualization In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDA53@SBS2K8.premierlab.local> This was years ago be we stained for the virus via IHC. The antibody was supplied by the client. That would seem to me to be the best method to determine deposits. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rodriguez, Kristofer Sent: Wednesday, October 22, 2014 9:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody deposit visualization One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine H&E will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an H&E. I was wondering if I should do any special stains that might show more than an H&E. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nguy0515 <@t> gmail.com Wed Oct 22 16:32:46 2014 From: nguy0515 <@t> gmail.com (GMail) Date: Wed Oct 22 16:32:51 2014 Subject: [Histonet] Processors Message-ID: Please help me decide on a processor, I am currently inquiring about refurbished processors for a small derm path lab with very low volumes. I have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k and a Leica TP1020 type 4 for $17. Which one would you recommend? What's durable and won't break down often? Should I go for the vip2000 compared to vip5 to save money? From ratliffjack <@t> hotmail.com Wed Oct 22 16:48:51 2014 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Oct 22 16:49:00 2014 Subject: [Histonet] Protocol of MMA Plastic section TRAP staining In-Reply-To: References: Message-ID: Hello Dorothy! I will forward you my protocol just as soon as I can get to my computer. On a side note, I no longer Chair the Hard Tissue Committee for the National Society for Histotechnology (NSH) as I have now been elected to the NSH Board of Directors as the Region III Director for the Southeast region. With that said, Sarah Mack is now the new Hard Tissue Committee Chairperson and Kim Simmons is the Education Development Manager for the NSH. I tell you this because Sarah is located in your part of the country and is currently the Histology Core Manager at University of Rochester Medical Center, Center for Musculosketeal Research. She works with bone and will be an good resource for you. I have copied her on this message. Kim Simmons will be helping to organize bone related educational materials and events for the NSH in the next year so she will be another good reference for you to follow. I have copied her to this message as well. I will still be a part of the Hard Tissue Committee in a supporting role. I just wanted you to know your additional options for information related to the histology of bone, biomaterials and medical device implants. Best Regards, Jack Ratliff > On Oct 22, 2014, at 3:20 PM, Dorothy Hu wrote: > > Dear Histoneters, > > Can someone email me your TRAP protocol for MMA section? I have one, but > staining is not consistent. No matter I stain for how long, ~ several hours > sometimes. There are always variations. I sincerely hope I can get > different protocol form you and make change. I remember saw other TRAP > protocol which is much longer than mine. > > The protocol I used was: > > 1. Dissolve 12mg Naphthol AS-MX phosphate (Sigma, N-5000) in 0.5ml N,N > methylformamide (Sigma,D8654). > 2. Mix above into 50 ml Sodium tartrate with 0.1M sodium acetate buffer at > pH5.0. > 3. Mix 30mg Fast Red Violet LB salt (Sigma, F3381) with above solution and > filter. > > Stain slides @37oC for at least one hour. The solution can be use for one > month when stored in 4oC. > Continue to do counterstain and mount slides. > Thank you. > > Dorothy Hu > MGH Endocrine histocore > Email: dhu5@partners.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From j.rowaihi <@t> alborglaboratories.com Thu Oct 23 03:25:23 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Thu Oct 23 03:25:27 2014 Subject: [Histonet] Processors In-Reply-To: References: Message-ID: <012f01cfee9a$e5a05ce0$b0e116a0$@rowaihi@alborglaboratories.com> hi colleague if your daily processed specimens less than hundred, go ahead for VIP5. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GMail Sent: Thursday, October 23, 2014 12:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processors Please help me decide on a processor, I am currently inquiring about refurbished processors for a small derm path lab with very low volumes. I have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k and a Leica TP1020 type 4 for $17. Which one would you recommend? What's durable and won't break down often? Should I go for the vip2000 compared to vip5 to save money? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Oct 23 03:44:25 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Oct 23 03:44:41 2014 Subject: AW: [Histonet] Antibody deposit visualization In-Reply-To: References: Message-ID: <000001cfee9d$8d84f1d0$a88ed570$@gmx.at> Hi, I think this is comparable with IF for detecting human autoimmun antibodies in skin or kidney. The human immunglobulins are detected with an fluorochrome conjugated antibody mouse-anti-human IgG (for example). In your case it may be successful to stain with a goat-anti-xxx IgG / IgM /IgA etc. On human FFPE kidney we performed pretreatment with pepsin (3-5 min) and then 30 min polyclonal anti-serum 1:10 and 1:20. To show immunglobulin-deposits in the glomeruli you can perform a trichrome-stain like MSB or SFOG. Protein deposits will be shown bright red, but are not distinguishable in the kind of protein. It will depend on the amount of deposit, I assume. Hope this helps Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rodriguez, Kristofer Gesendet: Mittwoch, 22. Oktober 2014 17:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Antibody deposit visualization One of my clients has been performing experiments with a mouse model of Dengue Virus Infection. They have been treating the mice with monoclonal antibodies (client did not specify what antibody)as a potential treatment for dengue virus infection. They have harvested the kidneys and put them in 10% NBF. They want to know if routine H&E will show any antibody deposits. I told him that I would not be able to directly observe the antibody deposit itself, however I might be able to observe the reaction to the antibody deposit in the basement membrane of the glomeruli as a membranous glomerulopathy on an H&E. I was wondering if I should do any special stains that might show more than an H&E. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Neelam.Joshi <@t> nephropath.com Thu Oct 23 07:42:21 2014 From: Neelam.Joshi <@t> nephropath.com (Neelam Joshi) Date: Thu Oct 23 07:42:26 2014 Subject: [Histonet] Double Labeling EM Blocks Message-ID: Hello, Does anyone has a procedure for double labeling EM Blocks with two identifier? Please let me know as soon as possible. Thanks you all! Neelam Joshi,HT From koellingr <@t> comcast.net Thu Oct 23 08:37:10 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Oct 23 08:37:29 2014 Subject: [Histonet] Double Labeling EM Blocks In-Reply-To: References: Message-ID: <1384569836.13537882.1414071430839.JavaMail.root@comcast.net> Neelam, If you are referring to immunolabelling for transmission electron microscopy, it is relatively simple to apply and this worked wonderfully for projects we did, to use 2 antibodies to two different epitopes but each antibody with a different size gold particle attached.? The gold particles are between a few nanometers up to 30 or 40 nanometers in diameter which are attached to antibodies and so are easy to tell apart from one another. ? Ray Lake Forest Park, WA ----- Original Message ----- From: "Neelam Joshi" To: histonet@lists.utsouthwestern.edu Sent: Thursday, October 23, 2014 5:42:21 AM Subject: [Histonet] Double Labeling EM Blocks Hello, Does anyone has a procedure for double labeling EM Blocks with two identifier? Please let me know as soon as possible. Thanks you all! Neelam Joshi,HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV <@t> mail.amc.edu Thu Oct 23 09:13:39 2014 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Thu Oct 23 09:13:45 2014 Subject: [Histonet] O Fix Message-ID: Hi everyone, I have been asked to post a few questions regarding O FIx. Is anyone using this for their lymph node cases? Any pros or cons? What is the fixation time versus 10% NBF ? Have you had any staining issues on H&E, special stains or Immunohistochemistry ? Does it require special disposal method? Phew !!! I think that's all of the questions... any help would be greatly appreciated. Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 23 09:23:58 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Oct 23 09:24:19 2014 Subject: [Histonet] RE: Double Labeling EM Blocks In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367B1524@ex07.net.ucsf.edu> Neelam, we don't have two ids on the EM blocks themselves, but we do print a label from our lab computer system that has patient name and case number that is applied to the box that holds the blocks. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Neelam Joshi Sent: Thursday, October 23, 2014 5:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Double Labeling EM Blocks Hello, Does anyone has a procedure for double labeling EM Blocks with two identifier? Please let me know as soon as possible. Thanks you all! Neelam Joshi,HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Thu Oct 23 10:09:03 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Oct 23 10:09:40 2014 Subject: [Histonet] RE: Double Labeling EM Blocks In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B1524@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B1524@ex07.net.ucsf.edu> Message-ID: <124333381.13675454.1414076943576.JavaMail.root@comcast.net> Oh, oh, sorry. I goofed.? I thought this "double labeling" was technical and not regulatory. Ray Lake Forest Park ----- Original Message ----- From: "Timothy Morken" To: "Neelam Joshi" , histonet@lists.utsouthwestern.edu Sent: Thursday, October 23, 2014 7:23:58 AM Subject: [Histonet] RE: Double Labeling EM Blocks Neelam, we don't have two ids on the EM blocks themselves, but we do print a label from our lab computer system that has patient name and case number that is applied to the box that holds the blocks. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Neelam Joshi Sent: Thursday, October 23, 2014 5:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Double Labeling EM Blocks Hello, Does anyone has a procedure for double labeling EM Blocks with two identifier? Please let me know as soon as possible. Thanks you all! Neelam Joshi,HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lsmallwo <@t> ppmh.org Thu Oct 23 12:27:59 2014 From: lsmallwo <@t> ppmh.org (Smallwood, Lorraine) Date: Thu Oct 23 12:28:22 2014 Subject: [Histonet] Working on Saturday Message-ID: We are a 450 bed hospital. Due to our size and volume of work, our work week is Monday-Saturday. Because of healthcare reform, budget cuts and staffing shortage, we are faced with closing the department on Saturday (!!Histotechs are happy!!). One tech will continue to come in on Saturday to process any breast specimens received. I am curious to find out what is the norm concerning working on Saturdays. How many other Histology departments work on Saturday? Thanks in advance for your opinions! Lorraine Smallwood MScEd, PA(ASCP) BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT) Pathologist Assistant Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. From Valerie.Hannen <@t> parrishmed.com Thu Oct 23 12:33:03 2014 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Thu Oct 23 12:35:29 2014 Subject: [Histonet] RE: Working on Saturday In-Reply-To: References: Message-ID: <450B7A81EDA0C54E97C53D60F00776C323373841BE@isexstore03> We only come in for the processing of breasts as well. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smallwood, Lorraine Sent: Thursday, October 23, 2014 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Working on Saturday We are a 450 bed hospital. Due to our size and volume of work, our work week is Monday-Saturday. Because of healthcare reform, budget cuts and staffing shortage, we are faced with closing the department on Saturday (!!Histotechs are happy!!). One tech will continue to come in on Saturday to process any breast specimens received. I am curious to find out what is the norm concerning working on Saturdays. How many other Histology departments work on Saturday? Thanks in advance for your opinions! Lorraine Smallwood MScEd, PA(ASCP) BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT) Pathologist Assistant Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From Joyce.Weems <@t> emoryhealthcare.org Thu Oct 23 12:44:22 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Oct 23 12:44:32 2014 Subject: [Histonet] RE: Working on Saturday In-Reply-To: References: Message-ID: We stopped Sat a few years ago. We have someone on call if something is needed. We trained the clinical lab to take the breast cases off the processor so we rarely need to come in. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smallwood, Lorraine Sent: Thursday, October 23, 2014 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Working on Saturday We are a 450 bed hospital. Due to our size and volume of work, our work week is Monday-Saturday. Because of healthcare reform, budget cuts and staffing shortage, we are faced with closing the department on Saturday (!!Histotechs are happy!!). One tech will continue to come in on Saturday to process any breast specimens received. I am curious to find out what is the norm concerning working on Saturdays. How many other Histology departments work on Saturday? Thanks in advance for your opinions! Lorraine Smallwood MScEd, PA(ASCP) BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT) Pathologist Assistant Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Toni.Rathborne <@t> rwjuh.edu Thu Oct 23 12:59:07 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Thu Oct 23 12:59:14 2014 Subject: [Histonet] RE: Working on Saturday In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED5970@vap1014.win.rwjuh.edu> Since ANP 22983 has been changed to state that breast fixation in formalin is between 6-72 hours, we no longer have staff come in on weekends. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, October 23, 2014 1:44 PM To: 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Working on Saturday We stopped Sat a few years ago. We have someone on call if something is needed. We trained the clinical lab to take the breast cases off the processor so we rarely need to come in. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smallwood, Lorraine Sent: Thursday, October 23, 2014 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Working on Saturday We are a 450 bed hospital. Due to our size and volume of work, our work week is Monday-Saturday. Because of healthcare reform, budget cuts and staffing shortage, we are faced with closing the department on Saturday (!!Histotechs are happy!!). One tech will continue to come in on Saturday to process any breast specimens received. I am curious to find out what is the norm concerning working on Saturdays. How many other Histology departments work on Saturday? Thanks in advance for your opinions! Lorraine Smallwood MScEd, PA(ASCP) BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT) Pathologist Assistant Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Elliott <@t> hli.ubc.ca Thu Oct 23 13:33:13 2014 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Thu Oct 23 13:33:15 2014 Subject: [Histonet] Fixation of gelatin scaffold with glutaraldehyde Message-ID: <5448E779020000D6000765BF@mail.hli.ubc.ca> Has anyone had any experience fixing gelatin scaffolds in glutaraldehyde vapour. One of the people I am working with is trying to do this and was wondering if anyone out there has had any experience doing this. Any suggestions would be greatly appreciated. Thanks Mark From Christy.Thompson <@t> dfo-mpo.gc.ca Thu Oct 23 13:38:40 2014 From: Christy.Thompson <@t> dfo-mpo.gc.ca (Thompson, Christy) Date: Thu Oct 23 13:38:49 2014 Subject: [Histonet] fish eggs: processing and fixation Message-ID: <63F107BCC37AEA49A75FD94AA3E07CB004C2E833@pacpbsex01.pac.dfo-mpo.ca> Hi all, I'm currently working on some unfertilized fish eggs. They were fixed in 10% NBF and processed in a vacuum processor with paraffin. The eggs are extremely hard to section and most of the yolk goes AWOL on the way to the water bath. I did do a search here for some help and a few things were posted but one post in particular found some answers but they were not listed in his reply and I think the post was pretty old (but no date on it). Anyone out there have any success with sectioning fish eggs? Thanks in advance! Christy Thompson A/Histology Lab Leader - Aquatic Animal Health Pacific Biological Station Fisheries and Oceans Canada Nanaimo, BC 250-756-7060 From wdesalvo.cac <@t> outlook.com Thu Oct 23 13:47:41 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu Oct 23 13:47:48 2014 Subject: [Histonet] Paraffin Used Message-ID: What paraffin do you use in your lab? Do you use different type for processing and embedding? Looking to investigate the top used paraffins for a workshop I am putting together. Sent from my iPhone From Joyce.Weems <@t> emoryhealthcare.org Thu Oct 23 13:48:45 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Oct 23 13:48:56 2014 Subject: [Histonet] RE: Working on Saturday In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED5970@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED5970@vap1014.win.rwjuh.edu> Message-ID: We get OP specimens that we need to take off sooner, but no problems with our clin lab help Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Rathborne, Toni [mailto:Toni.Rathborne@rwjuh.edu] Sent: Thursday, October 23, 2014 1:59 PM To: Weems, Joyce K.; 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu Subject: RE: Working on Saturday Since ANP 22983 has been changed to state that breast fixation in formalin is between 6-72 hours, we no longer have staff come in on weekends. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, October 23, 2014 1:44 PM To: 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Working on Saturday We stopped Sat a few years ago. We have someone on call if something is needed. We trained the clinical lab to take the breast cases off the processor so we rarely need to come in. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smallwood, Lorraine Sent: Thursday, October 23, 2014 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Working on Saturday We are a 450 bed hospital. Due to our size and volume of work, our work week is Monday-Saturday. Because of healthcare reform, budget cuts and staffing shortage, we are faced with closing the department on Saturday (!!Histotechs are happy!!). One tech will continue to come in on Saturday to process any breast specimens received. I am curious to find out what is the norm concerning working on Saturdays. How many other Histology departments work on Saturday? Thanks in advance for your opinions! Lorraine Smallwood MScEd, PA(ASCP) BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT) Pathologist Assistant Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From joelleweaver <@t> hotmail.com Thu Oct 23 14:05:23 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Thu Oct 23 14:05:29 2014 Subject: [Histonet] Processors In-Reply-To: <012f01cfee9a$e5a05ce0$b0e116a0$@rowaihi@alborglaboratories.com> References: , <012f01cfee9a$e5a05ce0$b0e116a0$@rowaihi@alborglaboratories.com> Message-ID: Agree Joelle Weaver MAOM, HTL (ASCP) QIHC > From: j.rowaihi@alborglaboratories.com > To: nguy0515@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 23 Oct 2014 11:25:23 +0300 > Subject: RE: [Histonet] Processors > CC: > > hi colleague > if your daily processed specimens less than hundred, go ahead for VIP5. > > > > Best Regards, > > > Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg > Medical Laboratories | Mobile +966 503629832| > j.rowaihi@alborglaboratories.com > Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | > Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | > www.alborglaboratories.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GMail > Sent: Thursday, October 23, 2014 12:33 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Processors > > Please help me decide on a processor, I am currently inquiring about > refurbished processors for a small derm path lab with very low volumes. I > have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k and a > Leica TP1020 type 4 for $17. Which one would you recommend? What's durable > and won't break down often? Should I go for the vip2000 compared to vip5 to > save money? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu Oct 23 14:08:39 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Oct 23 14:08:44 2014 Subject: [Histonet] fish eggs: processing and fixation In-Reply-To: <63F107BCC37AEA49A75FD94AA3E07CB004C2E833@pacpbsex01.pac.dfo-mpo.ca> References: <63F107BCC37AEA49A75FD94AA3E07CB004C2E833@pacpbsex01.pac.dfo-mpo.ca> Message-ID: Usually fish eggs are only processed by lightly salting and pressing. I've never tried sectioning them. Just serve with toast points and lemon wedges, maybe a little minced onion, and of course dry champagne! Sincerely? Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Oct 23, 2014 at 1:38 PM, Thompson, Christy < Christy.Thompson@dfo-mpo.gc.ca> wrote: > Hi all, I'm currently working on some unfertilized fish eggs. They were > fixed in 10% NBF and processed in a vacuum processor with paraffin. The > eggs are extremely hard to section and most of the yolk goes AWOL on the > way to the water bath. I did do a search here for some help and a few > things were posted but one post in particular found some answers but > they were not listed in his reply and I think the post was pretty old > (but no date on it). Anyone out there have any success with sectioning > fish eggs? > Thanks in advance! > > Christy Thompson > A/Histology Lab Leader - Aquatic Animal Health > Pacific Biological Station > Fisheries and Oceans Canada > Nanaimo, BC > 250-756-7060 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren <@t> gmail.com Thu Oct 23 14:18:09 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Oct 23 14:18:13 2014 Subject: [Histonet] Processors In-Reply-To: References: Message-ID: VIP5 all the way. IMHO any piece of equipment is useless without a warranty/service plan. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Oct 23, 2014 at 2:05 PM, Joelle Weaver wrote: > Agree > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > From: j.rowaihi@alborglaboratories.com > > To: nguy0515@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Thu, 23 Oct 2014 11:25:23 +0300 > > Subject: RE: [Histonet] Processors > > CC: > > > > hi colleague > > if your daily processed specimens less than hundred, go ahead for VIP5. > > > > > > > > Best Regards, > > > > > > Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg > > Medical Laboratories | Mobile +966 503629832| > > j.rowaihi@alborglaboratories.com > > Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | > > Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | > > www.alborglaboratories.com > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GMail > > Sent: Thursday, October 23, 2014 12:33 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Processors > > > > Please help me decide on a processor, I am currently inquiring about > > refurbished processors for a small derm path lab with very low volumes. I > > have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k > and a > > Leica TP1020 type 4 for $17. Which one would you recommend? What's > durable > > and won't break down often? Should I go for the vip2000 compared to vip5 > to > > save money? > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Thu Oct 23 14:20:58 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Oct 23 14:21:01 2014 Subject: [Histonet] Re: O Fix Message-ID: Vicki Gauch at AMCH in Albany, NY asks: >>I have been asked to post a few questions regarding O FIx. Is anyone using this for their lymph node cases? Any pros or cons? What is the fixation time versus 10% NBF? Have you had any staining issues on H&E, special stains or immunohistochemistry? Does it require special disposal method?<< Surgipath's O-Fix is described as containing formalin, acetic acid, and alcohol. It's probably fairly similar in composition to Davidson's fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde, 1 part glacial acetic acid). I use either of them as a "disclosing fixative" for finding mesenteric lymph nodes in colon resection specimens, and find them indistinguishable from each other. I use disclosing fixative in labs where I'm allowed to. It smells bad, and is difficult to use without adequate ventilation. To use them, you must have the specimen unfixed (brief exterior exposure to formalin is OK), cut off the mesenteries and cut them up, and fix overnight in the disclosing fixative. Microtomy, H & E, and the ordinary special stains work very well. I don't know about immunohistochemistry. Obviously you need to work closely with your pathologist to make the technique work. You'll have to check with your waste disposal people about disposing of disclosing fixatives. Bob Richmond Samurai Pathologist Maryville TN From Christy.Thompson <@t> dfo-mpo.gc.ca Thu Oct 23 15:37:26 2014 From: Christy.Thompson <@t> dfo-mpo.gc.ca (Thompson, Christy) Date: Thu Oct 23 15:37:36 2014 Subject: [Histonet] fish eggs: processing and fixation In-Reply-To: References: <63F107BCC37AEA49A75FD94AA3E07CB004C2E833@pacpbsex01.pac.dfo-mpo.ca> Message-ID: <63F107BCC37AEA49A75FD94AA3E07CB00FF5AB61@pacpbsex01.pac.dfo-mpo.ca> Sadly we don't have the capacity to do frozen sections :( Christy -----Original Message----- From: Diane G. Miller [mailto:dgmiller@coho.net] Sent: October 23, 2014 11:56 AM To: Thompson, Christy Subject: Re: [Histonet] fish eggs: processing and fixation Have you tried doing frozen sections instead of processing them in paraffin. We use to do frozen sections on frog eggs that were fixed and it worked picking them up on charged slides. Diane -----Original Message----- From: Thompson, Christy Sent: Thursday, October 23, 2014 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish eggs: processing and fixation Hi all, I'm currently working on some unfertilized fish eggs. They were fixed in 10% NBF and processed in a vacuum processor with paraffin. The eggs are extremely hard to section and most of the yolk goes AWOL on the way to the water bath. I did do a search here for some help and a few things were posted but one post in particular found some answers but they were not listed in his reply and I think the post was pretty old (but no date on it). Anyone out there have any success with sectioning fish eggs? Thanks in advance! Christy Thompson A/Histology Lab Leader - Aquatic Animal Health Pacific Biological Station Fisheries and Oceans Canada Nanaimo, BC 250-756-7060 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans <@t> histologistics.com Thu Oct 23 15:39:34 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Oct 23 15:39:41 2014 Subject: [Histonet] peroxidase staining for plants Message-ID: Hello All, I am not familiar with staining plants for peroxidase, so I thought I would ask you. Does anyone out there do peroxidase staining on plants? I have done myeloperoxidase staining on animal tissue. Is this the same for plants? Most of the plant antibody sites I have gone to do not list peroxides as a test. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From marktarango <@t> gmail.com Thu Oct 23 15:41:15 2014 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Oct 23 15:41:19 2014 Subject: [Histonet] ALK IHC Message-ID: Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark From bcooper <@t> chla.usc.edu Thu Oct 23 17:36:50 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Oct 23 17:36:56 2014 Subject: [Histonet] Paraffin Used In-Reply-To: References: Message-ID: Hi Bill, We infiltrate with Paraplast, and embed with Paraplast Extra. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 23, 2014 11:48 AM To: histonet Subject: [Histonet] Paraffin Used What paraffin do you use in your lab? Do you use different type for processing and embedding? Looking to investigate the top used paraffins for a workshop I am putting together. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From liz <@t> premierlab.com Thu Oct 23 17:53:09 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Oct 23 17:53:15 2014 Subject: [Histonet] Paraffin Used In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDA8A@SBS2K8.premierlab.local> We do the same here. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Thursday, October 23, 2014 4:37 PM To: WILLIAM DESALVO; histonet Subject: RE: [Histonet] Paraffin Used Hi Bill, We infiltrate with Paraplast, and embed with Paraplast Extra. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 23, 2014 11:48 AM To: histonet Subject: [Histonet] Paraffin Used What paraffin do you use in your lab? Do you use different type for processing and embedding? Looking to investigate the top used paraffins for a workshop I am putting together. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> skm.org.pk Thu Oct 23 23:47:31 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Thu Oct 23 23:45:45 2014 Subject: [Histonet] Paraffin Used In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECDA8A@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECDA8A@SBS2K8.premierlab.local> Message-ID: We infiltrate and embed with Paraplast Plus Surgipath. Regards, Muhammad Tahseen MLT (JIMTEF) Japan Histology Supervisor SKMCH&RC Lahore Pakistan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, October 24, 2014 3:53 AM To: Cooper, Brian; WILLIAM DESALVO; histonet Subject: RE: [Histonet] Paraffin Used We do the same here. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Thursday, October 23, 2014 4:37 PM To: WILLIAM DESALVO; histonet Subject: RE: [Histonet] Paraffin Used Hi Bill, We infiltrate with Paraplast, and embed with Paraplast Extra. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, October 23, 2014 11:48 AM To: histonet Subject: [Histonet] Paraffin Used What paraffin do you use in your lab? Do you use different type for processing and embedding? Looking to investigate the top used paraffins for a workshop I am putting together. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> skm.org.pk Thu Oct 23 23:55:21 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Thu Oct 23 23:53:37 2014 Subject: [Histonet] RE: Working on Saturday In-Reply-To: References: Message-ID: Dear Lorraine, Our Histology departments work on Saturday. Muhammad Tahseen MLT (JIMTEF) Japan Histology Supervisor SKMCH&RC Lahore Pakistan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smallwood, Lorraine Sent: Thursday, October 23, 2014 10:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Working on Saturday We are a 450 bed hospital. Due to our size and volume of work, our work week is Monday-Saturday. Because of healthcare reform, budget cuts and staffing shortage, we are faced with closing the department on Saturday (!!Histotechs are happy!!). One tech will continue to come in on Saturday to process any breast specimens received. I am curious to find out what is the norm concerning working on Saturdays. How many other Histology departments work on Saturday? Thanks in advance for your opinions! Lorraine Smallwood MScEd, PA(ASCP) BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT) Pathologist Assistant Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> skm.org.pk Fri Oct 24 00:00:39 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Thu Oct 23 23:59:48 2014 Subject: [Histonet] RE: Double Labeling EM Blocks In-Reply-To: References: Message-ID: We are labeling all Blocks and slides with two identifier as hospital policy. Regards Muhammad Tahseen MLT (JIMTEF) Japan Histology Supervisor SKMCH&RC Lahore Pakistan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Neelam Joshi Sent: Thursday, October 23, 2014 5:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Double Labeling EM Blocks Hello, Does anyone has a procedure for double labeling EM Blocks with two identifier? Please let me know as soon as possible. Thanks you all! Neelam Joshi,HT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nina.pries <@t> skane.se Fri Oct 24 01:32:00 2014 From: nina.pries <@t> skane.se (Pries Nina) Date: Fri Oct 24 01:32:22 2014 Subject: [Histonet] Pipette calibration with Artel PCS? Message-ID: Hi. I'm not sure if Histonet is the best place to ask, but I was wondering if anyone out there is using - or have heard about - the Artel PCS system for pipette calibration as an alternative to the traditional gravimetric method? My work place is in the process of setting up its own pipette calibration instead of sending them away, and since we are out of good spaces to place a balance (climate controlled+vibration free+ergonomically OK) we are looking into options such as the PCS. The cost of aquiring the system would equal the cost of getting a balance with a table, and the PCS is said to be more accurate at the small volumes we use. Reagents are costly though... Any experiences? Nina Pries Dep of Clinical Pathology, Lund, Sweden From d.a.faichney <@t> stir.ac.uk Fri Oct 24 03:26:10 2014 From: d.a.faichney <@t> stir.ac.uk (Debbie Faichney) Date: Fri Oct 24 03:26:30 2014 Subject: [Histonet] RE: fish eggs: processing and fixation In-Reply-To: <63F107BCC37AEA49A75FD94AA3E07CB004C2E833@pacpbsex01.pac.dfo-mpo.ca> References: <63F107BCC37AEA49A75FD94AA3E07CB004C2E833@pacpbsex01.pac.dfo-mpo.ca> Message-ID: <8ED3F2CA5B78E142B8193376C57330F801163632CBFB@EXCH2007.ad.stir.ac.uk> Hi Christy, Do you have the facility for resin sectioning? This is the way to go for fish eggs. It's a long time since this type of processing was carried out in our labs but if you are able to process in this way I'm sure I would be able to dig out a protocol from our Thesis library. I also suspect 10%NBF will have to be replaced with another fixative. Regards Debbie Technical Specialist Fish Health and Welfare Laboratories Institute of Aquaculture University of Stirling Stirling, FK9 4LA Tel: +44(1)1786 466592/466590 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thompson, Christy Sent: 23 October 2014 19:39 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish eggs: processing and fixation Hi all, I'm currently working on some unfertilized fish eggs. They were fixed in 10% NBF and processed in a vacuum processor with paraffin. The eggs are extremely hard to section and most of the yolk goes AWOL on the way to the water bath. I did do a search here for some help and a few things were posted but one post in particular found some answers but they were not listed in his reply and I think the post was pretty old (but no date on it). Anyone out there have any success with sectioning fish eggs? Thanks in advance! Christy Thompson A/Histology Lab Leader - Aquatic Animal Health Pacific Biological Station Fisheries and Oceans Canada Nanaimo, BC 250-756-7060 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling has been ranked in the top 12 of UK universities for graduate employment*. 94% of our 2012 graduates were in work and/or further study within six months of graduation. *The Telegraph The University of Stirling is a charity registered in Scotland, number SC 011159. From tbraud <@t> holyredeemer.com Fri Oct 24 08:16:43 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Oct 24 08:16:51 2014 Subject: [Histonet] RE: working on Saturday In-Reply-To: <20141024053816.05DA71E8D38@trendmess-svr.holyredeemer.local> References: <20141024053816.05DA71E8D38@trendmess-svr.holyredeemer.local> Message-ID: We are a 242 bed hospital with a very active maternity unit and a huge women's health service. We get approx 10,000 cases/year. We find no advantage to working on Saturdays with the exception of a STAT cytology for an inhouse patient. Also, with the new recommended fixation times for breasts, we no longer even have one person for that little bit of Saturday coverage. There was not one peep from any physicians. Even when I used to manage a hospital lab twice the size, we did not cover Saturdays. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 1. Working on Saturday (Smallwood, Lorraine) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From PAMarcum <@t> uams.edu Fri Oct 24 08:57:43 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Oct 24 08:57:49 2014 Subject: [Histonet] RE: working on Saturday In-Reply-To: References: <20141024053816.05DA71E8D38@trendmess-svr.holyredeemer.local> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA320125289451@Mail2Node2.ad.uams.edu> We do have someone come in on Saturday for half a day. We have an average of 35 bone marrows a day and getting them cut and ready on Saturday is what we have to do to make Monday work. This is a teaching hospital and the residents love to submit everything but the kitchen sink on Fridays and Saturday mornings. We also have GI and Gyn clinics on Friday and the biopsy load goes up along with routine surgicals so Mondays can be very heavy. The person working the half day Saturday can pick day during the week to leave early. We have the same person on call for the week to cover RUSH transplant biopsies. (They rarely have to come in however; we still have to cover if needed.) The on call person answers any problems with processors or other issues. When we are full staff the cover is every fifth week, so it is not too bad. I cover for any open spaces or health issues. They prefer the supervisor not take call to avoid overtime. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Friday, October 24, 2014 8:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: working on Saturday We are a 242 bed hospital with a very active maternity unit and a huge women's health service. We get approx 10,000 cases/year. We find no advantage to working on Saturdays with the exception of a STAT cytology for an inhouse patient. Also, with the new recommended fixation times for breasts, we no longer even have one person for that little bit of Saturday coverage. There was not one peep from any physicians. Even when I used to manage a hospital lab twice the size, we did not cover Saturdays. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 1. Working on Saturday (Smallwood, Lorraine) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From abtdhu <@t> gmail.com Fri Oct 24 09:03:17 2014 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Fri Oct 24 09:03:24 2014 Subject: [Histonet] Re: Histonet Digest, Vol 131, Issue 26 In-Reply-To: <5449f289.aa35b60a.31c9.fffffe41SMTPIN_ADDED_MISSING@mx.google.com> References: <5449f289.aa35b60a.31c9.fffffe41SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: *Regarding processors* *I also need to help on determine which processor for our core. Our majority tissue is bone. * *Right now, we like VIP serials because of vacuum/pressure plus agitation. Please help me* *on this VIP processor, I never used this kind one. We are very small volume* *core, also do frozen and plastic undecal bone. We have a Shandon Excelsior now and feel the * *vacuum volume for this 10 years old processor is not enough for bone. * *Any input for this will be greatly appreciated. * *Dorothy* *MGH endocrine histocore* > > 9. RE: Processors (Joelle Weaver) > > 11. Re: Processors (Jay Lundgren) > > > > Message: 9 > Date: Thu, 23 Oct 2014 19:05:23 +0000 > From: Joelle Weaver > Subject: RE: [Histonet] Processors > To: Jamal , 'GMail' > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Agree > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > From: j.rowaihi@alborglaboratories.com > > To: nguy0515@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Thu, 23 Oct 2014 11:25:23 +0300 > > Subject: RE: [Histonet] Processors > > CC: > > > > hi colleague > > if your daily processed specimens less than hundred, go ahead for VIP5. > > > > > > > > Best Regards, > > > > > > Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg > > Medical Laboratories | Mobile +966 503629832| > > j.rowaihi@alborglaboratories.com > > Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | > > Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | > > www.alborglaboratories.com > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GMail > > Sent: Thursday, October 23, 2014 12:33 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Processors > > > > Please help me decide on a processor, I am currently inquiring about > > refurbished processors for a small derm path lab with very low volumes. I > > have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k > and a > > Leica TP1020 type 4 for $17. Which one would you recommend? What's > durable > > and won't break down often? Should I go for the vip2000 compared to vip5 > to > > save money? > > > Sincerely? > Jay A. Lundgren, > M.S., HTL (ASCP) > > > > From Dorothy.L.Webb <@t> HealthPartners.Com Fri Oct 24 10:51:57 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Oct 24 10:52:03 2014 Subject: [Histonet] paraffin and Saturday work Message-ID: <65d07b4222424a6a8766fd070fa99ae8@HPEXCH06.HealthPartners.int> We use Leica infiltration paraffin for the processors and their Blue Ribbon for embedding We work Saturdays to lighten the workload on Mondays or it is overwhelming. We are a 24/6 lab (no techs in lab Saturday after 3:30and until 0400 Mondays) Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From tejohnson <@t> genoptix.com Fri Oct 24 10:51:45 2014 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Fri Oct 24 10:52:06 2014 Subject: [Histonet] Re: Working Saturday Message-ID: <94f3eabd24a746d59a33cb365967b895@PHUSCB-SP37MB03.genoptix.org> I applaud labs who have moved away from Saturday rotations. But I'm really at a loss to understand how it cuts costs? Don't most of you shift your work week for your staff if filling a Saturday rotation? So long as you don't pay OT for a Saturday, wouldn't the costs be the same? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From PAMarcum <@t> uams.edu Fri Oct 24 11:01:50 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Oct 24 11:02:14 2014 Subject: [Histonet] RE: Re: Working Saturday In-Reply-To: <94f3eabd24a746d59a33cb365967b895@PHUSCB-SP37MB03.genoptix.org> References: <94f3eabd24a746d59a33cb365967b895@PHUSCB-SP37MB03.genoptix.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3201252894C4@Mail2Node2.ad.uams.edu> We give a half day during the week for the half day worked on Saturday. This ended the overtime. Since we start at 4AM generally the person on call will have some day during the week they want to leave early. Since we have the schedule up before the first of the month most can make appointments or personal things for that half day. It cut down on time off during work hours and allowed us to only pay call pay if the person is needed during an overnight. Our shifts start at 4AM and we close at 4:30PM so they only cove the open hours. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, October 24, 2014 10:52 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Working Saturday I applaud labs who have moved away from Saturday rotations. But I'm really at a loss to understand how it cuts costs? Don't most of you shift your work week for your staff if filling a Saturday rotation? So long as you don't pay OT for a Saturday, wouldn't the costs be the same? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 24 11:56:03 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Oct 24 12:11:58 2014 Subject: [Histonet] RE: Re: Working Saturday In-Reply-To: <94f3eabd24a746d59a33cb365967b895@PHUSCB-SP37MB03.genoptix.org> References: <94f3eabd24a746d59a33cb365967b895@PHUSCB-SP37MB03.genoptix.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367B19CD@ex07.net.ucsf.edu> You are right terry, it does not save in costs and just shift the workload. However, we staff one tech on Saturday to cover rush transplant bx That tech takes the previouis Monday off. Because we have to pay 3 hours minimum for any call back we simply staff it 8 hours. The Saturday tech embeds and cuts all the bx received Friday (100 to 150 blocks), does the same-day transplant rush, any Friday overnight rush, and load the processors with blocks from Saturday grossing (a catchup day for residents. ). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, October 24, 2014 8:52 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: Working Saturday I applaud labs who have moved away from Saturday rotations. But I'm really at a loss to understand how it cuts costs? Don't most of you shift your work week for your staff if filling a Saturday rotation? So long as you don't pay OT for a Saturday, wouldn't the costs be the same? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Oct 24 12:16:26 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Oct 24 12:16:31 2014 Subject: [Histonet] IFN gamma Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDA91@SBS2K8.premierlab.local> Happy Friday!! I was wondering if the status of IHC staining for IFN gamma has changed since Chris Van Der Loos published the article back in 2001. Back then he stated that it was not possible to stain for IFN gamma via IHC. Immunohistochemical Detection of Interferon-gamma Fake or Fact? Chris M. van der Loos, Mischa A. Houtkamp, Onno J. de Boer, Peter Teeling, Allard C. van der Wal, and Anton E. Becker Academic Medical Center, Department of Cardiovascular Pathology, Amsterdam, The Netherlands Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From hardin <@t> oncology.wisc.edu Fri Oct 24 14:22:10 2014 From: hardin <@t> oncology.wisc.edu (Joe Hardin) Date: Fri Oct 24 14:22:16 2014 Subject: [Histonet] cryosectioning hydrogel Message-ID: <544AA6E2.9040300@oncology.wisc.edu> I am having a difficult time sectioning hydrogel @ 10u on the cryostat. Does anyone have a helpful suggestion? From joelleweaver <@t> hotmail.com Fri Oct 24 14:34:22 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Oct 24 14:34:27 2014 Subject: [Histonet] ALK IHC In-Reply-To: References: Message-ID: Recently all the pathologists I work with prefer the ALK FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Thu, 23 Oct 2014 13:41:15 -0700 > From: marktarango@gmail.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] ALK IHC > > Does anyone stain lung cancer specimens for ALK using IHC? If not, any > opinions? > > thanks > > Mark > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Oct 24 14:49:26 2014 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Oct 24 14:49:31 2014 Subject: [Histonet] ALK IHC In-Reply-To: References: Message-ID: Did you ever run ALK IHC on lung cancer cases or have they always used FISH? thanks Mark Tarango On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver wrote: > Recently all the pathologists I work with prefer the ALK FISH. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > Date: Thu, 23 Oct 2014 13:41:15 -0700 > > From: marktarango@gmail.com > > To: Histonet@lists.utsouthwestern.edu > > CC: > > Subject: [Histonet] ALK IHC > > > > > Does anyone stain lung cancer specimens for ALK using IHC? If not, any > > opinions? > > > > thanks > > > > Mark > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Richard.Cartun <@t> hhchealth.org Fri Oct 24 15:15:24 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Oct 24 15:15:52 2014 Subject: [Histonet] ALK IHC In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355E3AF3@HHCEXCHMB03.hhcsystem.org> We are in the process of bringing this on-line. We are using a rabbit monoclonal (clone D5F3) from Cell Signaling Technologies (Danvers, MA). There are cases that are obviously positive and then there cases that are "Equivocal" (like HER2) where you need to do the FISH test. The advantages of the IHC are less cost, faster result, can be used on specimens with limited tumor cells present, and it may prove useful for those tumors that show genomic heterogeneity. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, October 23, 2014 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Joyce.Weems <@t> emoryhealthcare.org Fri Oct 24 15:29:42 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Oct 24 15:30:10 2014 Subject: [Histonet] ALK IHC In-Reply-To: References: Message-ID: We've always done FISH.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Friday, October 24, 2014 3:49 PM To: Joelle Weaver Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ALK IHC Did you ever run ALK IHC on lung cancer cases or have they always used FISH? thanks Mark Tarango On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver wrote: > Recently all the pathologists I work with prefer the ALK FISH. > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > Date: Thu, 23 Oct 2014 13:41:15 -0700 > > From: marktarango@gmail.com > > To: Histonet@lists.utsouthwestern.edu > > CC: > > Subject: [Histonet] ALK IHC > > > > > Does anyone stain lung cancer specimens for ALK using IHC? If not, > > any opinions? > > > > thanks > > > > Mark > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From joelleweaver <@t> hotmail.com Fri Oct 24 15:47:39 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Oct 24 15:47:45 2014 Subject: [Histonet] ALK IHC In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355E3AF3@HHCEXCHMB03.hhcsystem.org> References: , <9215BD4B0BA1B44D962A71C758B68D2E355E3AF3@HHCEXCHMB03.hhcsystem.org> Message-ID: I think it is probably better with the rabbit monoclonal IHC than maybe in the past. IHC is MUCH cheaper, easier and faster than the FISH. Doing ALK FISH manually was easier than Her2 FISH manual, but still takes much more time than any IHC, and is expensive ( but necessary for certain cases). Seems like the IHC would be nice screen with a reflex to FISH. Joelle Weaver MAOM, HTL (ASCP) QIHC From: Richard.Cartun@hhchealth.org To: marktarango@gmail.com; Histonet@lists.utsouthwestern.edu Date: Fri, 24 Oct 2014 20:15:24 +0000 Subject: RE: [Histonet] ALK IHC CC: We are in the process of bringing this on-line. We are using a rabbit monoclonal (clone D5F3) from Cell Signaling Technologies (Danvers, MA). There are cases that are obviously positive and then there cases that are "Equivocal" (like HER2) where you need to do the FISH test. The advantages of the IHC are less cost, faster result, can be used on specimens with limited tumor cells present, and it may prove useful for those tumors that show genomic heterogeneity. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Thursday, October 23, 2014 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ALK IHC Does anyone stain lung cancer specimens for ALK using IHC? If not, any opinions? thanks Mark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Oct 24 15:48:28 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Oct 24 15:48:31 2014 Subject: [Histonet] ALK IHC In-Reply-To: References: , , , Message-ID: I can tell you that my biggest trouble in all dealing with ALK was finding enough positive cases. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Joyce.Weems@emoryhealthcare.org > To: marktarango@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ALK IHC > Date: Fri, 24 Oct 2014 20:29:42 +0000 > > We've always done FISH.. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango > Sent: Friday, October 24, 2014 3:49 PM > To: Joelle Weaver > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ALK IHC > > Did you ever run ALK IHC on lung cancer cases or have they always used FISH? > > thanks > > Mark Tarango > > On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver > wrote: > > > Recently all the pathologists I work with prefer the ALK FISH. > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > > > > > > > Date: Thu, 23 Oct 2014 13:41:15 -0700 > > > From: marktarango@gmail.com > > > To: Histonet@lists.utsouthwestern.edu > > > CC: > > > Subject: [Histonet] ALK IHC > > > > > > > > Does anyone stain lung cancer specimens for ALK using IHC? If not, > > > any opinions? > > > > > > thanks > > > > > > Mark > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). From joelleweaver <@t> hotmail.com Fri Oct 24 15:48:44 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Oct 24 15:48:48 2014 Subject: [Histonet] ALK IHC In-Reply-To: References: , , , Message-ID: I can tell you that my biggest trouble in all dealing with ALK was finding enough positive cases. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Joyce.Weems@emoryhealthcare.org > To: marktarango@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ALK IHC > Date: Fri, 24 Oct 2014 20:29:42 +0000 > > We've always done FISH.. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango > Sent: Friday, October 24, 2014 3:49 PM > To: Joelle Weaver > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ALK IHC > > Did you ever run ALK IHC on lung cancer cases or have they always used FISH? > > thanks > > Mark Tarango > > On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver > wrote: > > > Recently all the pathologists I work with prefer the ALK FISH. > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > > > > > > > Date: Thu, 23 Oct 2014 13:41:15 -0700 > > > From: marktarango@gmail.com > > > To: Histonet@lists.utsouthwestern.edu > > > CC: > > > Subject: [Histonet] ALK IHC > > > > > > > > Does anyone stain lung cancer specimens for ALK using IHC? If not, > > > any opinions? > > > > > > thanks > > > > > > Mark > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). From joelleweaver <@t> hotmail.com Fri Oct 24 15:48:49 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Oct 24 15:49:16 2014 Subject: [Histonet] ALK IHC In-Reply-To: References: , , , Message-ID: I can tell you that my biggest trouble in all dealing with ALK was finding enough positive cases. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Joyce.Weems@emoryhealthcare.org > To: marktarango@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ALK IHC > Date: Fri, 24 Oct 2014 20:29:42 +0000 > > We've always done FISH.. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango > Sent: Friday, October 24, 2014 3:49 PM > To: Joelle Weaver > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ALK IHC > > Did you ever run ALK IHC on lung cancer cases or have they always used FISH? > > thanks > > Mark Tarango > > On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver > wrote: > > > Recently all the pathologists I work with prefer the ALK FISH. > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > > > > > > > Date: Thu, 23 Oct 2014 13:41:15 -0700 > > > From: marktarango@gmail.com > > > To: Histonet@lists.utsouthwestern.edu > > > CC: > > > Subject: [Histonet] ALK IHC > > > > > > > > Does anyone stain lung cancer specimens for ALK using IHC? If not, > > > any opinions? > > > > > > thanks > > > > > > Mark > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). From joelleweaver <@t> hotmail.com Fri Oct 24 15:50:24 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Oct 24 15:50:48 2014 Subject: [Histonet] ALK IHC In-Reply-To: References: , , , Message-ID: I can tell you that my biggest trouble in all dealing with ALK was finding enough positive cases. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Joyce.Weems@emoryhealthcare.org > To: marktarango@gmail.com; joelleweaver@hotmail.com > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] ALK IHC > Date: Fri, 24 Oct 2014 20:29:42 +0000 > > We've always done FISH.. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango > Sent: Friday, October 24, 2014 3:49 PM > To: Joelle Weaver > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] ALK IHC > > Did you ever run ALK IHC on lung cancer cases or have they always used FISH? > > thanks > > Mark Tarango > > On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver > wrote: > > > Recently all the pathologists I work with prefer the ALK FISH. > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > > > > > > > Date: Thu, 23 Oct 2014 13:41:15 -0700 > > > From: marktarango@gmail.com > > > To: Histonet@lists.utsouthwestern.edu > > > CC: > > > Subject: [Histonet] ALK IHC > > > > > > > > Does anyone stain lung cancer specimens for ALK using IHC? If not, > > > any opinions? > > > > > > thanks > > > > > > Mark > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). From j.rowaihi <@t> alborglaboratories.com Sat Oct 25 03:11:11 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Sat Oct 25 03:11:26 2014 Subject: [Histonet] Re: Histonet Digest, Vol 131, Issue 26 In-Reply-To: References: <5449f289.aa35b60a.31c9.fffffe41SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <004401cff02b$3e7be280$bb73a780$@rowaihi@alborglaboratories.com> Hi I am still recommending VIP5 for you I have: Tissue Tek VIP5 Jr Leica AS 6025 And Leica TP1020 Both of Tissue Tek and Leica are very good but according to your little volume (less than 100 cassette daily), VIP5 Jr are your suitable machine. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Hu Sent: Friday, October 24, 2014 5:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 131, Issue 26 *Regarding processors* *I also need to help on determine which processor for our core. Our majority tissue is bone. * *Right now, we like VIP serials because of vacuum/pressure plus agitation. Please help me* *on this VIP processor, I never used this kind one. We are very small volume* *core, also do frozen and plastic undecal bone. We have a Shandon Excelsior now and feel the * *vacuum volume for this 10 years old processor is not enough for bone. * *Any input for this will be greatly appreciated. * *Dorothy* *MGH endocrine histocore* > > 9. RE: Processors (Joelle Weaver) > > 11. Re: Processors (Jay Lundgren) > > > > Message: 9 > Date: Thu, 23 Oct 2014 19:05:23 +0000 > From: Joelle Weaver > Subject: RE: [Histonet] Processors > To: Jamal , 'GMail' > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Agree > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > From: j.rowaihi@alborglaboratories.com > > To: nguy0515@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Thu, 23 Oct 2014 11:25:23 +0300 > > Subject: RE: [Histonet] Processors > > CC: > > > > hi colleague > > if your daily processed specimens less than hundred, go ahead for VIP5. > > > > > > > > Best Regards, > > > > > > Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg > > Medical Laboratories | Mobile +966 503629832| > > j.rowaihi@alborglaboratories.com > > Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | > > Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | > > www.alborglaboratories.com > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GMail > > Sent: Thursday, October 23, 2014 12:33 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Processors > > > > Please help me decide on a processor, I am currently inquiring about > > refurbished processors for a small derm path lab with very low volumes. I > > have quotes for VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k > and a > > Leica TP1020 type 4 for $17. Which one would you recommend? What's > durable > > and won't break down often? Should I go for the vip2000 compared to vip5 > to > > save money? > > > Sincerely? > Jay A. Lundgren, > M.S., HTL (ASCP) > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nguy0515 <@t> gmail.com Sat Oct 25 15:16:01 2014 From: nguy0515 <@t> gmail.com (GMail) Date: Sat Oct 25 15:16:15 2014 Subject: [Histonet] Salary exempt/nonexempt status Message-ID: Can someone please help me, I've been losing sleep over this ever since moving to CA and working here. If you are paid a salary but NOT a supervisor or manager are you considered exempt or nonexempt? I was told by someone that if you are salary but not in a managerial position you are considered nonexempt and are entitled to earn OT. I have tried searching the laws in CA and the only thing I could find is: 1. If you earn twice the wage of minimum wage you are exempt. 2. If you are a type of professional (doctors and lawyers etc) or in a managerial position you are exempt With that being said, I believe a lot of people in the U.S with other professions make twice the wage of #1, so does that mean they are all exempt? Please help me answer this question! Thank you in advance! Sent from my iPhone From LSebree <@t> uwhealth.org Sat Oct 25 16:38:19 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Sat Oct 25 16:38:25 2014 Subject: [Histonet] Salary exempt/nonexempt status In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF0E33D2@UWHC-MBX12.uwhis.hosp.wisc.edu> I fought this very same battle having been salaried for many years and gradually working more and more OT. I worked solely with hourly employees and was not a manager or supervisor. Everyone I worked with accrued OT pay but I did not and I was usually working the most OT. I contacted our state office of labor and workforce development and learned about the exempt/nonexempt statuses. Upon bringing this information to both my institution's personnel department and eventually my manager, they reviewed my position description along with several other employees. They resolved the issue by making me and some other people hourly with no cut in pay so now I still work some overtime but accrue OT pay. I also received back pay from 2 years of overtime. Maybe CA has a labor department that could help you as well. Good luck, Linda A. Sebree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of GMail [nguy0515@gmail.com] Sent: Saturday, October 25, 2014 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Salary exempt/nonexempt status Can someone please help me, I've been losing sleep over this ever since moving to CA and working here. If you are paid a salary but NOT a supervisor or manager are you considered exempt or nonexempt? I was told by someone that if you are salary but not in a managerial position you are considered nonexempt and are entitled to earn OT. I have tried searching the laws in CA and the only thing I could find is: 1. If you earn twice the wage of minimum wage you are exempt. 2. If you are a type of professional (doctors and lawyers etc) or in a managerial position you are exempt With that being said, I believe a lot of people in the U.S with other professions make twice the wage of #1, so does that mean they are all exempt? Please help me answer this question! Thank you in advance! Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Sat Oct 25 17:29:43 2014 From: b427297 <@t> aol.com (b427297@aol.com) Date: Sat Oct 25 17:29:52 2014 Subject: [Histonet] Salary exempt/nonexempt status In-Reply-To: <77DD817201982748BC67D7960F2F76AF0E33D2@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF0E33D2@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <611A974E-3E22-425F-ACEB-7BED83A1CE66@aol.com> Are you paid a. by the hour or was your pay quoted to you by b. yearly salary? C. Do you fill out a time card to record your hours? If A and C are yes, you are non exempt. Your human resource department should have the answer. If you don't have an HR dept and you cant ask your boss to clarify, then you have a crummy relationship with your boss, and should look for a better job. Sent from my iPhone > On Oct 25, 2014, at 4:38 PM, Sebree Linda A wrote: > > I fought this very same battle having been salaried for many years and gradually working more and more OT. I worked solely with hourly employees and was not a manager or supervisor. Everyone I worked with accrued OT pay but I did not and I was usually working the most OT. I contacted our state office of labor and workforce development and learned about the exempt/nonexempt statuses. Upon bringing this information to both my institution's personnel department and eventually my manager, they reviewed my position description along with several other employees. They resolved the issue by making me and some other people hourly with no cut in pay so now I still work some overtime but accrue OT pay. I also received back pay from 2 years of overtime. > > Maybe CA has a labor department that could help you as well. > > Good luck, > > Linda A. Sebree > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of GMail [nguy0515@gmail.com] > Sent: Saturday, October 25, 2014 3:16 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Salary exempt/nonexempt status > > Can someone please help me, I've been losing sleep over this ever since moving to CA and working here. > > If you are paid a salary but NOT a supervisor or manager are you considered exempt or nonexempt? > > I was told by someone that if you are salary but not in a managerial position you are considered nonexempt and are entitled to earn OT. > > I have tried searching the laws in CA and the only thing I could find is: 1. If you earn twice the wage of minimum wage you are exempt. 2. If you are a type of professional (doctors and lawyers etc) or in a managerial position you are exempt > > With that being said, I believe a lot of people in the U.S with other professions make twice the wage of #1, so does that mean they are all exempt? > > Please help me answer this question! > > Thank you in advance! > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Oct 25 18:18:16 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Oct 25 18:18:22 2014 Subject: [Histonet] Salary exempt/nonexempt status In-Reply-To: References: Message-ID: <340569122.429823.1414279097009.JavaMail.yahoo@jws100209.mail.ne1.yahoo.com> hings are usually different in CA but in FL this is more or less as it works:1-?if you are Supervisor or Manager, even when you have to clock in/out no matter how many ours extra you work, you do not get paid overtime over your salary.2- if you are?"bench worker" you clock in/out and IF you work more than 40 hours week the difference = overtime if you work less than 40 hours week = your are paid for the worked hours. Even if you some days work??>8 hours you may be scheduled to work <8 hours some other days to compensate.I remember that when I retired I calculated that I had worked?in excess of 3?YEARS unpaid because I was manager (life is tough!)Ren? J.? On Saturday, October 25, 2014 4:16 PM, GMail wrote: Can someone please help me, I've been losing sleep over this ever since moving to CA and working here. If you are paid a salary but NOT a supervisor or manager are you considered exempt or nonexempt? I was told by someone that if you are salary but not in a managerial position you are considered nonexempt and are entitled to earn OT. I have tried searching the laws in CA and the only thing I could find is: 1. If you earn twice the wage of minimum wage you are exempt. 2.? If you are a type of professional (doctors and lawyers etc) or in a managerial position you are exempt With that being said, I believe a lot of people in the U.S with other professions make twice the wage of #1, so does that mean they are all exempt? Please help me answer this question! Thank you in advance! Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.bernard <@t> comcast.net Sun Oct 26 10:57:13 2014 From: ian.bernard <@t> comcast.net (ian bernard) Date: Sun Oct 26 10:57:49 2014 Subject: [Histonet] Proficiency Testing (PT) or Interlaboratory Educational Comparisons or Education Program Message-ID: <01eb01cff135$845530d0$8cff9270$@comcast.net> Are these terms interchangeable one in the same for Anatomic Pathology or is PT just for the Clinical side of the house? We participate with the PIP for Surgical Pathology, ASCP Case Reports for Non-GYN Cytopathology and the HistoQip for two of its series. Inputs. IB From djemge <@t> aol.com Sun Oct 26 13:05:10 2014 From: djemge <@t> aol.com (Djemge) Date: Sun Oct 26 13:35:47 2014 Subject: [Histonet] hello Message-ID: <8D1BF5E84926664-E80-14625@webmail-va166.sysops.aol.com> http://thesoundsrecords.com/Jim.php?4sidzckpqle023 Donna Emge djemge@aol.com ----------- It's not real love until you let your girlfriend use your toothbrush. From j.rowaihi <@t> alborglaboratories.com Mon Oct 27 01:27:20 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Mon Oct 27 01:27:22 2014 Subject: [Histonet] Proficiency Testing (PT) or Interlaboratory Education Comparisons or Education Program In-Reply-To: <01eb01cff135$845530d0$8cff9270$@comcast.net> References: <01eb01cff135$845530d0$8cff9270$@comcast.net> Message-ID: <008701cff1af$11426b10$33c74130$@rowaihi@alborglaboratories.com> Hi According to my understanding and practicing, the PT must cover all your test menu: Surgical Pathology, Cytopathology & Immunohistochemistry. ANP.02000 Education Participation Phase I As applicable, the laboratory participates in a peer educational program in anatomic pathology (e.g. CAP Educational Anatomic Pathology Programs). NOTE: The laboratory should consider participation in programs appropriate to its scope of service. Such programs provide valuable educational opportunities for peer performance comparisons in both technical and diagnostic arenas. While none of these completely emulates the precise clinical setting involving anatomic pathology preparations and rendering of anatomic or clinical diagnoses, they can be a useful benchmark of peer-based performance in a national database. Evidence of Compliance: ? Records such as CAP order form, purchase order AND ? Completed/submitted results indicating that the laboratory is participating in a CAP educational AP program OR records of enrollment/participation in another AP peer educational program OR records for participation in a laboratory-developed program by circulating case material with other laboratories or within the laboratory's own practice with documentation of peer review CYP.00125 PT Participation Phase II For laboratories subject to US regulations that perform gynecologic cytopathology, the laboratory and all individuals who examine gynecologic preparations participate in the CAP Gynecologic Cytology PT Program (PAP PT) or another proficiency testing program in gynecologic cytopathology approved by CMS. NOTE: This checklist requirement applies only to US laboratories and other laboratories subject to CLIA regulations. Laboratories must maintain documentation of PT performance for at least 2 years. Documentation must be kept for each individual participating in annual PT, including identification of those who are retested; documentation of remedial training; records of imposition of limitations on slide examination; and records of re-examination of slides, as required by CLIA. Evidence of Compliance: ? Written policy describing handling of PT failures (may include retesting, remedial training, and imposition of limitations on slide examination) AND ? Records that the laboratory is enrolled and all currently employed personnel have successfully completed PT AND ? Records of retesting, remedial training and imposition of limitations, if applicable AND ? Records of notification to the PT provider and CMS for any PAP testing personnel who left employment prior to completion of annual PT REFERENCES 1) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Extension of certain effective dates for clinical laboratory requirements and personnel requirements for cytologists. Fed Register. 1994(Dec 6):62609 [42CFR493.855] Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ian bernard Sent: Sunday, October 26, 2014 6:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Proficiency Testing (PT) or Interlaboratory Educational Comparisons or Education Program Are these terms interchangeable one in the same for Anatomic Pathology or is PT just for the Clinical side of the house? We participate with the PIP for Surgical Pathology, ASCP Case Reports for Non-GYN Cytopathology and the HistoQip for two of its series. Inputs. IB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa <@t> alliedsearchpartners.com Mon Oct 27 08:47:30 2014 From: melissa <@t> alliedsearchpartners.com (Melissa Owens (Phelan)) Date: Mon Oct 27 08:47:49 2014 Subject: [Histonet] Histology Manager job in North Carolina- Message-ID: Good Morning, I wanted to spread the word that I have a direct hire opportunity for a histology manager in North Carolina that I have recently been contacted to fill. Please contact me for more details if you are interested. To view a complete list of Allied Search Partners current openings go to: http://www.jobs.net/jobs/alliedsearchpartners/en-us/all-jobs/United-States/ Melissa Owens (Phelan) President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 F: 888.388.7572 From relia1 <@t> earthlink.net Mon Oct 27 11:36:18 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Oct 27 11:36:28 2014 Subject: [Histonet] RELIA Histology Job Alert RELIA EXCLUSIVES Hot off the presses!!!!! Message-ID: <022b01cff204$22e52fb0$68af8f10$@earthlink.net> Hi Histonetters!! I hope you all had a great weekend. I wanted to put up a quick post to give you a heads up on some new opportunities that are about to go online and wanted to let you know before they got out to the general public. If you are interested great! If you know someone who might be interested that's great too because if you refer someone to RELIA and we place them you will earn a referral fee and who can't use a little extra green these days right? Pardon me for posting the actual ads but I wanted to get the information to you right away. Here is the info: Histology Supervisor - Northern Arizona RELIA Solutions the nation's only recruiting firm specializing in the permanent placement of histology Professionals has been engaged exclusively in a search for a talented accomplished and driven histology supervisor To lead a team of motivated and committed histology professionals providing excellent patient care. This is a state of the art lab in a beautiful area and our client offers a very competitive salary, benefits and relocation assistance along with top notch support from upper management. ASCP HT/HTL and 3-5 years of supervisory experience in histology lab environment along with routine histology and Immunohistochemistry expertise. For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Senior Histotechnologist - Northern Arizona RELIA Solutions the nation's only recruiting firm specializing in the permanent placement of histology Professionals has been engaged exclusively in a search for an experienced driven senior histotechnologist. Become the Right hand person to the manager leading a team of motivated and committed histology professionals providing excellent patient care. If you desire a move into management in your future this could be the job for you this is a state of the art lab in a beautiful area and our client offers a very competitive salary, benefits and relocation assistance along with top notch support from upper management. ASCP HT/HTL, 3-5 years of routine histology and Immunohistochemistry expertise; along with the initiative to take the lead and get things done are the skills and and experience we are looking for. For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. Keywords: histology, histologist, histotechnician, histotechnologist, I would love to place you or one of your friends in either of these positions and I always appreciate you taking the time to read my posts. Have a great week! Thanks-Pam Right Place, Right Time, Right Move witRELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From monettes <@t> mskcc.org Mon Oct 27 12:11:18 2014 From: monettes <@t> mskcc.org (monettes@mskcc.org) Date: Mon Oct 27 12:11:44 2014 Subject: [Histonet] Position Posting: Laboratory Manager, Lab of Comparative Pathology, MSKCC/WCMC/RU, New York, NY Message-ID: <1E99C9D9F6F44A40BCD63551C0AB29E4B4F744E4@SMSKPEX10MBX3.MSKCC.ROOT.MSKCC.ORG> Position posting: Laboratory Manager, Lab. of Comparative Pathology, MSKCC/WCMC/RU, New York, NY The Laboratory of Comparative Pathology (LCP) is a full service diagnostic lab offering clinical and anatomic pathology services. The laboratory serves the research communities and the animal resources of Memorial Sloan Kettering Cancer Center, Weill Cornell Medical College and The Rockefeller University, by performing a wide range of laboratory procedures in Microbiology, Hematology, Chemistry, Parasitology, Urinalysis and Serology on specimens from a variety of animal species. It also provides histology and immunohistochemistry services to a team of four comparative pathologists and to the research community. To view more details about the position and to apply, please see: http://careers.mskcc.org/jobs/descriptions/manager-laboratory-of-comparative-pathology-new-york-new-york-job-4779417 S?bastien Monette, DMV, MVSc, DACVP Head of Anatomic Pathology Tri-Institutional Laboratory of Comparative Pathology Genetically Modified Animal Phenotyping Service Memorial Sloan-Kettering Cancer Center Weill Cornell Medical College The Rockefeller University 1275 York Avenue, Box 270, New York NY 10065 Phone: 646-888-2420 Fax: 646-422-0139 Email MSKCC: monettes@mskcc.org Email WCMC: sem2010@med.cornell.edu Web site: http://www.mskcc.org/research/comparative-medicine-pathology-0 ===================================================================== Please note that this e-mail and any files transmitted from Memorial Sloan-Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. From rmire <@t> cvpath.org Tue Oct 28 06:55:15 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Tue Oct 28 06:55:34 2014 Subject: [Histonet] Change in xylene substitute Message-ID: <000001cff2a6$11498ec0$33dcac40$@org> Hello netters, Are all Xylene Substitutes the same? My manager wants to change brands of Xylene Substitute to save money. My feeling is that I will need to validate processing, staining, ihc etc if the chemical formula is different from the substitute we currently use. If it's the same then no harm, no foul. What are your thoughts? Ronda From mpence <@t> grhs.net Tue Oct 28 08:12:43 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Oct 28 08:13:07 2014 Subject: [Histonet] RE: Working on Saturday In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED5970@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED5970@vap1014.win.rwjuh.edu> Message-ID: Same here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, October 23, 2014 1:01 PM To: 'Weems, Joyce K.'; 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Working on Saturday Since ANP 22983 has been changed to state that breast fixation in formalin is between 6-72 hours, we no longer have staff come in on weekends. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, October 23, 2014 1:44 PM To: 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Working on Saturday We stopped Sat a few years ago. We have someone on call if something is needed. We trained the clinical lab to take the breast cases off the processor so we rarely need to come in. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smallwood, Lorraine Sent: Thursday, October 23, 2014 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Working on Saturday We are a 450 bed hospital. Due to our size and volume of work, our work week is Monday-Saturday. Because of healthcare reform, budget cuts and staffing shortage, we are faced with closing the department on Saturday (!!Histotechs are happy!!). One tech will continue to come in on Saturday to process any breast specimens received. I am curious to find out what is the norm concerning working on Saturdays. How many other Histology departments work on Saturday? Thanks in advance for your opinions! Lorraine Smallwood MScEd, PA(ASCP) BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT) Pathologist Assistant Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Tue Oct 28 09:37:23 2014 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Tue Oct 28 09:37:30 2014 Subject: [Histonet] Combined Case prefix In-Reply-To: References: Message-ID: How many facilities have combined their prefixes? Meaning your pathology and cytology are using the same prefix so there is no difference when accessioning cases of pathology or cytology. What do you feel are the pro's and con's of this? Jessica Vacca From Timothy.Morken <@t> ucsfmedctr.org Tue Oct 28 09:56:20 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Oct 28 09:56:28 2014 Subject: [Histonet] RE: Combined Case prefix In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367B20A0@ex07.net.ucsf.edu> We have several prefixes: surgical, cytology, eye pathology, hemepath, autopsy, research (we have many more Specimen Classes but, thankfully, they do not all get separate prefixes). I'm not going to say it is absolutely necessary, just historical here. However, I have never been anywhere that did not have at least cyto and surgical use separate prefixes. I would guess that the larger the institution the more likely it is that there will be more separation. A small institution can combine everything without much problem. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Tuesday, October 28, 2014 7:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Combined Case prefix How many facilities have combined their prefixes? Meaning your pathology and cytology are using the same prefix so there is no difference when accessioning cases of pathology or cytology. What do you feel are the pro's and con's of this? Jessica Vacca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Tue Oct 28 10:15:36 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Oct 28 10:15:43 2014 Subject: [Histonet] RE: Combined Case prefix In-Reply-To: References: Message-ID: We have a few different prefixes. We use Meditech and have different enter/edit screens depending on whether the specimen is a cytology or surgical. It also help in searches and gathering statistics if you can limit to a specific prefix. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Tuesday, October 28, 2014 10:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Combined Case prefix How many facilities have combined their prefixes? Meaning your pathology and cytology are using the same prefix so there is no difference when accessioning cases of pathology or cytology. What do you feel are the pro's and con's of this? Jessica Vacca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From TanyaAbbott <@t> catholichealth.net Tue Oct 28 11:04:33 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Tue Oct 28 11:05:15 2014 Subject: [Histonet] Storing specimens Message-ID: <852F7D2C14FB464D80E182B15DB138AF394F8FFC@CHIEX005.CHI.catholichealth.net> How does everyone handle storage of specimens for 2 weeks post sign out? We are a small lab (as is everyone on Histonet, I'm sure!!) and trying to figure out the best way to organize to make the flow go smoothly. Meaning, we are hoping not to have to check each specimen to determine it was signed out before we discard. Any suggestions? Thanks! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Ronald.Houston <@t> nationwidechildrens.org Tue Oct 28 11:16:08 2014 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Oct 28 11:16:17 2014 Subject: [Histonet] TCR immunohistochemistry Message-ID: has anyone had any success with the antibodies available for TCR lymphocytes in human paraffin tissue? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From mjones <@t> metropath.com Tue Oct 28 11:42:49 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Oct 28 11:42:55 2014 Subject: [Histonet] Storing specimens Message-ID: We run a query of reports (finalized) for that period of two weeks, and any outstanding/pending report, we pull the bottle. We use the storage bins for each day of the week - during gross we toss the bottle into the storage bin for that day. We have two sets: Mon - Fri. We rotate those in two cabinets and have a ?Current? sign we use and move from one cabinet to another depending on which we dump. Once we dump the bins (and disinfect them) we keep the Medical Waste bin for another week (we keep our tissues for a total of three weeks) We don?t have storage room for 3 weeks worth rotation. Did I make any sense? :) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 10/28/14, 10:04 AM, "Abbott, Tanya" wrote: >How does everyone handle storage of specimens for 2 weeks post sign out? >We are a small lab (as is everyone on Histonet, I'm sure!!) and trying to >figure out the best way to organize to make the flow go smoothly. >Meaning, we are hoping not to have to check each specimen to determine it >was signed out before we discard. Any suggestions? >Thanks! Tanya > >Tanya G. Abbott RT (CSMLS) >Manager Technologist, Histology/Cytology >St. Joseph Medical Center >Reading, PA 19603-0316 >ph 610-378-2635 >fax 610-898-5871 >email: tanyaabbott@catholichealth.net > >This electronic mail and any attached documents are intended solely for >the named addressee(s) and contain confidential information. If you are >not an addressee, or responsible for delivering this email to an >addressee, you have received this email in error and are notified that >reading, copying, or disclosing this email is prohibited. If you received >this email in error, immediately reply to the sender and delete the >message completely from your computer system. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kukiezada <@t> upmc.edu Tue Oct 28 12:45:48 2014 From: kukiezada <@t> upmc.edu (Kukieza, Debra) Date: Tue Oct 28 12:45:58 2014 Subject: [Histonet] RE: Change in xylene substitute Message-ID: Hi Ronda, I was faced with the same issue a few years ago. We placed one gram of paraffin into equal amounts of the xylene substitutes and monitored how quickly and effectively the paraffin dissolved in each. In the end we proved it was better to keep the substitute we were using as it completely dissolved the paraffin and the other did not. The other actually left a rather sticky mess with the paraffin. Debra UPMC East Histology Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, October 28, 2014 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 131, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Position Posting: Laboratory Manager, Lab of Comparative Pathology, MSKCC/WCMC/RU, New York, NY (monettes@mskcc.org) 2. Change in xylene substitute (Ronda Mire) 3. RE: Working on Saturday (Mike Pence) 4. Combined Case prefix (Jessica.Vacca@HCAhealthcare.com) 5. RE: Combined Case prefix (Morken, Timothy) 6. RE: Combined Case prefix (Tom McNemar) 7. Storing specimens (Abbott, Tanya) 8. TCR immunohistochemistry (Houston, Ronald) 9. Re: Storing specimens (Michael Ann Jones) ---------------------------------------------------------------------- Message: 1 Date: Mon, 27 Oct 2014 17:11:18 +0000 From: monettes@mskcc.org Subject: [Histonet] Position Posting: Laboratory Manager, Lab of Comparative Pathology, MSKCC/WCMC/RU, New York, NY To: histonet@lists.utsouthwestern.edu Message-ID: <1E99C9D9F6F44A40BCD63551C0AB29E4B4F744E4@SMSKPEX10MBX3.MSKCC.ROOT.MSKCC.ORG> Content-Type: text/plain; charset=iso-8859-1 Position posting: Laboratory Manager, Lab. of Comparative Pathology, MSKCC/WCMC/RU, New York, NY The Laboratory of Comparative Pathology (LCP) is a full service diagnostic lab offering clinical and anatomic pathology services. The laboratory serves the research communities and the animal resources of Memorial Sloan Kettering Cancer Center, Weill Cornell Medical College and The Rockefeller University, by performing a wide range of laboratory procedures in Microbiology, Hematology, Chemistry, Parasitology, Urinalysis and Serology on specimens from a variety of animal species. It also provides histology and immunohistochemistry services to a team of four comparative pathologists and to the research community. To view more details about the position and to apply, please see: http://careers.mskcc.org/jobs/descriptions/manager-laboratory-of-comparative-pathology-new-york-new-york-job-4779417 S?bastien Monette, DMV, MVSc, DACVP Head of Anatomic Pathology Tri-Institutional Laboratory of Comparative Pathology Genetically Modified Animal Phenotyping Service Memorial Sloan-Kettering Cancer Center Weill Cornell Medical College The Rockefeller University 1275 York Avenue, Box 270, New York NY 10065 Phone: 646-888-2420 Fax: 646-422-0139 Email MSKCC: monettes@mskcc.org Email WCMC: sem2010@med.cornell.edu Web site: http://www.mskcc.org/research/comparative-medicine-pathology-0 ===================================================================== Please note that this e-mail and any files transmitted from Memorial Sloan-Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. ------------------------------ Message: 2 Date: Tue, 28 Oct 2014 07:55:15 -0400 From: "Ronda Mire" Subject: [Histonet] Change in xylene substitute To: Message-ID: <000001cff2a6$11498ec0$33dcac40$@org> Content-Type: text/plain; charset="us-ascii" Hello netters, Are all Xylene Substitutes the same? My manager wants to change brands of Xylene Substitute to save money. My feeling is that I will need to validate processing, staining, ihc etc if the chemical formula is different from the substitute we currently use. If it's the same then no harm, no foul. What are your thoughts? Ronda ------------------------------ Message: 3 Date: Tue, 28 Oct 2014 13:12:43 +0000 From: Mike Pence Subject: [Histonet] RE: Working on Saturday To: "'Rathborne, Toni'" , "'Weems, Joyce K.'" , "'Smallwood, Lorraine'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Same here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, October 23, 2014 1:01 PM To: 'Weems, Joyce K.'; 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Working on Saturday Since ANP 22983 has been changed to state that breast fixation in formalin is between 6-72 hours, we no longer have staff come in on weekends. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, October 23, 2014 1:44 PM To: 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Working on Saturday We stopped Sat a few years ago. We have someone on call if something is needed. We trained the clinical lab to take the breast cases off the processor so we rarely need to come in. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smallwood, Lorraine Sent: Thursday, October 23, 2014 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Working on Saturday We are a 450 bed hospital. Due to our size and volume of work, our work week is Monday-Saturday. Because of healthcare reform, budget cuts and staffing shortage, we are faced with closing the department on Saturday (!!Histotechs are happy!!). One tech will continue to come in on Saturday to process any breast specimens received. I am curious to find out what is the norm concerning working on Saturdays. How many other Histology departments work on Saturday? Thanks in advance for your opinions! Lorraine Smallwood MScEd, PA(ASCP) BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT) Pathologist Assistant Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 28 Oct 2014 09:37:23 -0500 From: Subject: [Histonet] Combined Case prefix To: Message-ID: Content-Type: text/plain; charset="us-ascii" How many facilities have combined their prefixes? Meaning your pathology and cytology are using the same prefix so there is no difference when accessioning cases of pathology or cytology. What do you feel are the pro's and con's of this? Jessica Vacca ------------------------------ Message: 5 Date: Tue, 28 Oct 2014 14:56:20 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: Combined Case prefix To: "'Jessica.Vacca@HCAhealthcare.com'" , "Histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF367B20A0@ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" We have several prefixes: surgical, cytology, eye pathology, hemepath, autopsy, research (we have many more Specimen Classes but, thankfully, they do not all get separate prefixes). I'm not going to say it is absolutely necessary, just historical here. However, I have never been anywhere that did not have at least cyto and surgical use separate prefixes. I would guess that the larger the institution the more likely it is that there will be more separation. A small institution can combine everything without much problem. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Tuesday, October 28, 2014 7:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Combined Case prefix How many facilities have combined their prefixes? Meaning your pathology and cytology are using the same prefix so there is no difference when accessioning cases of pathology or cytology. What do you feel are the pro's and con's of this? Jessica Vacca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 28 Oct 2014 11:15:36 -0400 From: Tom McNemar Subject: [Histonet] RE: Combined Case prefix To: "'Jessica.Vacca@HCAhealthcare.com'" , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We have a few different prefixes. We use Meditech and have different enter/edit screens depending on whether the specimen is a cytology or surgical. It also help in searches and gathering statistics if you can limit to a specific prefix. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Tuesday, October 28, 2014 10:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Combined Case prefix How many facilities have combined their prefixes? Meaning your pathology and cytology are using the same prefix so there is no difference when accessioning cases of pathology or cytology. What do you feel are the pro's and con's of this? Jessica Vacca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 7 Date: Tue, 28 Oct 2014 16:04:33 +0000 From: "Abbott, Tanya" Subject: [Histonet] Storing specimens To: "histonet@lists.utsouthwestern.edu" Message-ID: <852F7D2C14FB464D80E182B15DB138AF394F8FFC@CHIEX005.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" How does everyone handle storage of specimens for 2 weeks post sign out? We are a small lab (as is everyone on Histonet, I'm sure!!) and trying to figure out the best way to organize to make the flow go smoothly. Meaning, we are hoping not to have to check each specimen to determine it was signed out before we discard. Any suggestions? Thanks! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 8 Date: Tue, 28 Oct 2014 16:16:08 +0000 From: "Houston, Ronald" Subject: [Histonet] TCR immunohistochemistry To: Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" has anyone had any success with the antibodies available for TCR lymphocytes in human paraffin tissue? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ------------------------------ Message: 9 Date: Tue, 28 Oct 2014 16:42:49 +0000 From: Michael Ann Jones Subject: Re: [Histonet] Storing specimens To: "Abbott, Tanya" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We run a query of reports (finalized) for that period of two weeks, and any outstanding/pending report, we pull the bottle. We use the storage bins for each day of the week - during gross we toss the bottle into the storage bin for that day. We have two sets: Mon - Fri. We rotate those in two cabinets and have a ?Current? sign we use and move from one cabinet to another depending on which we dump. Once we dump the bins (and disinfect them) we keep the Medical Waste bin for another week (we keep our tissues for a total of three weeks) We don?t have storage room for 3 weeks worth rotation. Did I make any sense? :) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 10/28/14, 10:04 AM, "Abbott, Tanya" wrote: >How does everyone handle storage of specimens for 2 weeks post sign out? >We are a small lab (as is everyone on Histonet, I'm sure!!) and trying >to figure out the best way to organize to make the flow go smoothly. >Meaning, we are hoping not to have to check each specimen to determine >it was signed out before we discard. Any suggestions? >Thanks! Tanya > >Tanya G. Abbott RT (CSMLS) >Manager Technologist, Histology/Cytology St. Joseph Medical Center >Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 >email: tanyaabbott@catholichealth.net > >This electronic mail and any attached documents are intended solely for >the named addressee(s) and contain confidential information. If you are >not an addressee, or responsible for delivering this email to an >addressee, you have received this email in error and are notified that >reading, copying, or disclosing this email is prohibited. If you >received this email in error, immediately reply to the sender and >delete the message completely from your computer system. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 131, Issue 31 ***************************************** From Valerie.Hannen <@t> parrishmed.com Tue Oct 28 12:49:25 2014 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Oct 28 12:50:50 2014 Subject: [Histonet] RE: Combined Case prefix In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B20A0@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B20A0@ex07.net.ucsf.edu> Message-ID: <450B7A81EDA0C54E97C53D60F00776C323373841BF@isexstore03> We only use one prefix for both AP and cytology( long ago we got rid of doing PAPS), has been this way for many years and don't recall any real problems. Our computer system can differentiate specimen types(fluids vs tissues) for searches that we have set up. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, October 28, 2014 10:56 AM To: 'Jessica.Vacca@HCAhealthcare.com'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Combined Case prefix We have several prefixes: surgical, cytology, eye pathology, hemepath, autopsy, research (we have many more Specimen Classes but, thankfully, they do not all get separate prefixes). I'm not going to say it is absolutely necessary, just historical here. However, I have never been anywhere that did not have at least cyto and surgical use separate prefixes. I would guess that the larger the institution the more likely it is that there will be more separation. A small institution can combine everything without much problem. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Tuesday, October 28, 2014 7:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Combined Case prefix How many facilities have combined their prefixes? Meaning your pathology and cytology are using the same prefix so there is no difference when accessioning cases of pathology or cytology. What do you feel are the pro's and con's of this? Jessica Vacca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From ihcman2010 <@t> hotmail.com Tue Oct 28 12:55:34 2014 From: ihcman2010 <@t> hotmail.com (Glen Dawson) Date: Tue Oct 28 12:55:43 2014 Subject: [Histonet] PT testing for ER/PR by IHC In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C323373841BF@isexstore03> References: , , <761E2B5697F795489C8710BCC72141FF367B20A0@ex07.net.ucsf.edu>, <450B7A81EDA0C54E97C53D60F00776C323373841BF@isexstore03> Message-ID: All, If you perform ER/PR staining by IHC, are you REQUIRED to participate in Proficiency Testing like that provided by CAP? Thank-you in advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Mercy Health System Janesville, WI From joelleweaver <@t> hotmail.com Tue Oct 28 13:21:00 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Tue Oct 28 13:21:14 2014 Subject: [Histonet] PT testing for ER/PR by IHC In-Reply-To: References: , , , , <761E2B5697F795489C8710BCC72141FF367B20A0@ex07.net.ucsf.edu>, , <450B7A81EDA0C54E97C53D60F00776C323373841BF@isexstore03>, Message-ID: yes Joelle Weaver MAOM, HTL (ASCP) QIHC > From: ihcman2010@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 28 Oct 2014 12:55:34 -0500 > Subject: RE: [Histonet] PT testing for ER/PR by IHC > > > All, > > > > If you perform ER/PR staining by IHC, are you REQUIRED to participate in Proficiency Testing like that provided by CAP? > > > > Thank-you in advance, > > > > Glen Dawson BS, HT(ASCP), QIHC > > Histology Technical Specialist > > Mercy Health System > > Janesville, WI > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin <@t> marshallmedical.org Tue Oct 28 14:04:27 2014 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Oct 28 14:04:39 2014 Subject: [Histonet] Telepathology Message-ID: <6ED9D4252F278841A0593D3D788AF24C2760C836@mailsvr.MARSHMED.local> We are being asked to provide evaluation of EUS (Endoscopic Ultra Sound) specimen from a remote location. We are provided with a microscope and a laptop, does anyone know of a simple way to capture and transmit images back to the pathology lab? Thank you. From amber.mckenzie <@t> gastrodocs.net Tue Oct 28 14:55:26 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Oct 28 14:55:40 2014 Subject: [Histonet] Squamous cells staining on H&E and IHC In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C2760C836@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C2760C836@mailsvr.MARSHMED.local> Message-ID: <5A33C952BB67F4468AF1F36D739212BC011255CDAD@JERRY.Gia.com> Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's? I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting? Change out water bath several times during shifts? Any suggestions? Thanks! From histo <@t> skm.org.pk Wed Oct 29 05:44:09 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Wed Oct 29 05:42:30 2014 Subject: [Histonet] RE: Squamous cells staining on H&E and IHC In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011255CDAD@JERRY.Gia.com> References: <6ED9D4252F278841A0593D3D788AF24C2760C836@mailsvr.MARSHMED.local> <5A33C952BB67F4468AF1F36D739212BC011255CDAD@JERRY.Gia.com> Message-ID: When the sections are cut and taken on the slide from water bath, slide must only holds on the frosted end of the slides don't touch other part of slide. Regards Muhammad Tahseen MLT (JIMTEF) Japan Histology Supervisor SKMCH&RC Lahore Pakistan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, October 29, 2014 12:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Squamous cells staining on H&E and IHC Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's? I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting? Change out water bath several times during shifts? Any suggestions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Wed Oct 29 07:50:42 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Wed Oct 29 07:50:48 2014 Subject: [Histonet] RE: Squamous cells staining on H&E and IHC Message-ID: We had this issue - particularly for our Keratin stain (as it highlights the squamous cells) We had much positive staining debris scattered on our slides. We cleaned our water baths diligently, made sure our techs were skimming between slides, and air current and stainers can bring debris as well. We kind of narrowed it down to one tech maybe? She got frustrated and doesn?t cut keratin controls anymore. Sadly, I?m not sure how/why this problem subsided. Following this discussion closely. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 10/29/14, 4:44 AM, "Pathology-Histology Sr. Supervisor" wrote: >When the sections are cut and taken on the slide from water bath, slide >must only holds on the frosted end of the slides don't touch other part >of slide. >Regards >Muhammad Tahseen >MLT (JIMTEF) Japan >Histology Supervisor >SKMCH&RC >Lahore >Pakistan > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber >McKenzie >Sent: Wednesday, October 29, 2014 12:55 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Squamous cells staining on H&E and IHC > > >Does anyone else have problems with what looks like squamous cells >staining on your H&E's and IHC's? I'm trying to figure out how to >eliminate that problem in our lab...wear gloves while cutting? Change >out water bath several times during shifts? Any suggestions? Thanks! > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tfountain <@t> dsmanitoba.ca Wed Oct 29 08:28:29 2014 From: tfountain <@t> dsmanitoba.ca (Tiana Baskin) Date: Wed Oct 29 08:28:50 2014 Subject: [Histonet] Bone Saw for surgical specimens Message-ID: Hi Histonetters, I am looking for a bone saw for smaller surgical specimens (like jaw bone). Any suggestions or input about what is working or not working for you would be helpful. Thanks in advance! -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From MMargiotta <@t> bmhmc.org Wed Oct 29 08:57:05 2014 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Wed Oct 29 08:57:10 2014 Subject: [Histonet] RE: Squamous cells staining on H&E and IHC In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011255CDAD@JERRY.Gia.com> References: <6ED9D4252F278841A0593D3D788AF24C2760C836@mailsvr.MARSHMED.local> <5A33C952BB67F4468AF1F36D739212BC011255CDAD@JERRY.Gia.com> Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC69CA1BC6@BMH-EXCHANGE-01.BMHMC.ORG> We have that problem mostly during the winter months when our hands get dry. Use hand lotion, that usually helps a bit. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, October 28, 2014 3:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Squamous cells staining on H&E and IHC Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's? I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting? Change out water bath several times during shifts? Any suggestions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From Reuel.Cornelia <@t> tsrh.org Wed Oct 29 09:25:26 2014 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Wed Oct 29 09:25:33 2014 Subject: [Histonet] RE: Squamous cells staining on H&E and IHC In-Reply-To: <230D0B9EC57D7A45A7A186C6AB4C7ABC69CA1BC6@BMH-EXCHANGE-01.BMHMC.ORG> References: <6ED9D4252F278841A0593D3D788AF24C2760C836@mailsvr.MARSHMED.local> <5A33C952BB67F4468AF1F36D739212BC011255CDAD@JERRY.Gia.com> <230D0B9EC57D7A45A7A186C6AB4C7ABC69CA1BC6@BMH-EXCHANGE-01.BMHMC.ORG> Message-ID: <5450B286.0F7E.00C5.1@tsrh.org> Wear gloves and do not scratch your head or nose with gloves on. >>> "Margiotta-Watz, Michele" 10/29/2014 8:57 AM >>> We have that problem mostly during the winter months when our hands get dry. Use hand lotion, that usually helps a bit. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, October 28, 2014 3:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Squamous cells staining on H&E and IHC Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's? I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting? Change out water bath several times during shifts? Any suggestions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AJohnson <@t> aipathology.com Wed Oct 29 11:19:03 2014 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Wed Oct 29 11:19:14 2014 Subject: [Histonet] FW: QIHC Message-ID: From: Amy Johnson Sent: Wednesday, October 29, 2014 6:57 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: QIHC Do you need to have experience with ISH to take the QIHC test? Thanks, Amylin Johnson, BS HTL(ASCP) Associates in Pathology Wausau, WI 715-847-2130 From Karen.Heckford <@t> DignityHealth.org Wed Oct 29 12:04:29 2014 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed Oct 29 12:04:48 2014 Subject: [Histonet] slides for IHC's Message-ID: I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From Karen.Heckford <@t> DignityHealth.org Wed Oct 29 12:08:03 2014 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed Oct 29 12:08:20 2014 Subject: [Histonet] Cap ER/PR Message-ID: Good Morning, Apparently while I was out on disability a CAP ER/PR PM2-A 2014 did not get done. Somehow one of our pathologists got it and forgot she had it. That being said, does anyone have a equivalent test they do instead. We only do this CAP testing to show competency. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From Timothy.Morken <@t> ucsfmedctr.org Wed Oct 29 12:14:38 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Oct 29 12:18:25 2014 Subject: [Histonet] RE: Cap ER/PR In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367B278D@ex07.net.ucsf.edu> Karen, when this has happened in the past CAP has told me to run the test and score it, then when the survey summary comes with the scores and discussion, to have the pathologist review and score the answers and document the review. It's about the best you can do when something gets missed. You may want to have two reviewers sign off in this case. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cap ER/PR Good Morning, Apparently while I was out on disability a CAP ER/PR PM2-A 2014 did not get done. Somehow one of our pathologists got it and forgot she had it. That being said, does anyone have a equivalent test they do instead. We only do this CAP testing to show competency. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Oct 29 13:47:33 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Oct 29 13:47:47 2014 Subject: [Histonet] Re: squamous cell contamination on slides Message-ID: <001a01cff3a8$ceb41f40$6c1c5dc0$@bresnan.net> After following this thread on a topic that has always been a pet peeve and a problem at times, a few suggestions. This is a problem discussed many times on Histonet. If people are using the water bath as a "finger bowl", they need to learn to not let fingers go "snorkeling" when picking up sections. This is sloppy, poor technique. Anyone can learn to never touch the water surface with bare skin. I have seen people immerse their fingers up to the first joint and their stained sections were covered by a snowstorm of squamous cells. Be aware that squamous cells are going to exfoliate from other than hands, so not touching face and hair is good advice even if one wears gloves. Hand lotion is helpful except for those slather on lotion or the lotion is particularly heavy duty and then they still touch the water. This can cause a double problem - an oil slick which is a terrible section adhesive along with squamous cells from bare skin. Hold slide at top or on sides, as mentioned previously. Wear gloves. Not always popular with a common argument one loses dexterity handling slides. If gloves do not flop around loosely but fit the hand well, then dexterity is not lost. Good luck Gayle Callis HTL/HT/MT(ASCP) **************************************************************************** *************************************************** We have that problem mostly during the winter months when our hands get dry. Use hand lotion, that usually helps a bit. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 -----Original Message----- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto: histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, October 28, 2014 3:55 PM To: histonet <@t> lists.utsouthwestern.edu Subject: [Histonet] Squamous cells staining on H&E and IHC Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's? I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting? Change out water bath several times during shifts? Any suggestions? Thanks! From TGoins <@t> mt.gov Wed Oct 29 13:53:11 2014 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Oct 29 13:53:41 2014 Subject: [Histonet] RE: slides for IHC's In-Reply-To: References: Message-ID: Mercedes Medical Starfrost adhesive for IHC and HE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne <@t> rwjuh.edu Wed Oct 29 14:38:17 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Wed Oct 29 14:38:25 2014 Subject: [Histonet] RE: slides for IHC's In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED6D6C@vap1014.win.rwjuh.edu> Leica Apex slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, October 29, 2014 2:53 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Mercedes Medical Starfrost adhesive for IHC and HE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> luriechildrens.org Wed Oct 29 14:48:37 2014 From: NMargaryan <@t> luriechildrens.org (Margaryan, Naira) Date: Wed Oct 29 14:49:19 2014 Subject: [Histonet] Re: squamous cell contamination on slides In-Reply-To: <001a01cff3a8$ceb41f40$6c1c5dc0$@bresnan.net> References: <001a01cff3a8$ceb41f40$6c1c5dc0$@bresnan.net> Message-ID: <34B2EDA118548A4EB35D6E650345BA643D5FB1D2@SV-EX11.childrensmemorial.org> Hi Hestonetters, I need to do IHC of SC4MOL on FFPE sections. If any of you have been done this staining before, please send me your procedure. Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Wednesday, October 29, 2014 1:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: squamous cell contamination on slides After following this thread on a topic that has always been a pet peeve and a problem at times, a few suggestions. This is a problem discussed many times on Histonet. If people are using the water bath as a "finger bowl", they need to learn to not let fingers go "snorkeling" when picking up sections. This is sloppy, poor technique. Anyone can learn to never touch the water surface with bare skin. I have seen people immerse their fingers up to the first joint and their stained sections were covered by a snowstorm of squamous cells. Be aware that squamous cells are going to exfoliate from other than hands, so not touching face and hair is good advice even if one wears gloves. Hand lotion is helpful except for those slather on lotion or the lotion is particularly heavy duty and then they still touch the water. This can cause a double problem - an oil slick which is a terrible section adhesive along with squamous cells from bare skin. Hold slide at top or on sides, as mentioned previously. Wear gloves. Not always popular with a common argument one loses dexterity handling slides. If gloves do not flop around loosely but fit the hand well, then dexterity is not lost. Good luck Gayle Callis HTL/HT/MT(ASCP) **************************************************************************** *************************************************** We have that problem mostly during the winter months when our hands get dry. Use hand lotion, that usually helps a bit. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 -----Original Message----- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto: histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, October 28, 2014 3:55 PM To: histonet <@t> lists.utsouthwestern.edu Subject: [Histonet] Squamous cells staining on H&E and IHC Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's? I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting? Change out water bath several times during shifts? Any suggestions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mroark <@t> sfmc.net Wed Oct 29 15:05:44 2014 From: mroark <@t> sfmc.net (Matthew D. Roark) Date: Wed Oct 29 15:05:53 2014 Subject: [Histonet] RE: slides for IHC's In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED6D6C@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED6D6C@vap1014.win.rwjuh.edu> Message-ID: <7ab8364572044fa0a27e5640d7ac71cc@BL2PR04MB195.namprd04.prod.outlook.com> We also use Leica Apex slides. Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, October 29, 2014 2:38 PM To: 'Goins, Tresa'; Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Leica Apex slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, October 29, 2014 2:53 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Mercedes Medical Starfrost adhesive for IHC and HE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Wed Oct 29 15:09:07 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Wed Oct 29 15:09:16 2014 Subject: [Histonet] RE: slides for IHC's In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED6D6C@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED6D6C@vap1014.win.rwjuh.edu> Message-ID: Thermofisher Superfrost Plus. Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, October 29, 2014 12:38 PM To: 'Goins, Tresa'; Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Leica Apex slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, October 29, 2014 2:53 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Mercedes Medical Starfrost adhesive for IHC and HE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From histotex <@t> sbcglobal.net Wed Oct 29 18:22:29 2014 From: histotex <@t> sbcglobal.net (Sherry Martin) Date: Wed Oct 29 18:29:15 2014 Subject: [Histonet] Re: squamous cell contamination on slides In-Reply-To: <34B2EDA118548A4EB35D6E650345BA643D5FB1D2@SV-EX11.childrensmemorial.org> References: <001a01cff3a8$ceb41f40$6c1c5dc0$@bresnan.net> <34B2EDA118548A4EB35D6E650345BA643D5FB1D2@SV-EX11.childrensmemorial.org> Message-ID: <1414624949.21966.YahooMailNeo@web185303.mail.gq1.yahoo.com> I've also seen this problem in a larger lab where lab aides printed the slides. Make sure anyone handling slides handles them by the edges or top or wears gloves. I've even had it myself, when I had to hand-label slides. I switched to wearing a glove on the writing hand and the squamous cells disappeared! Sherry On Wednesday, October 29, 2014 2:48 PM, "Margaryan, Naira" wrote: Hi Hestonetters, I need to do IHC of SC4MOL on FFPE sections. If any of you have been done this staining before, please send me your procedure. Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Wednesday, October 29, 2014 1:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: squamous cell contamination on slides After following this thread on a topic that has always been a pet peeve and a problem at times, a few suggestions. This is a problem discussed many times on Histonet. If people are using the water bath as a "finger bowl", they need to learn to not let fingers go "snorkeling" when picking up sections. This is sloppy, poor technique. Anyone can learn to never touch the water surface with bare skin. I have seen people immerse their fingers up to the first joint and their stained sections were covered by a snowstorm of squamous cells. Be aware that squamous cells are going to exfoliate from other than hands, so not touching face and hair is good advice even if one wears gloves. Hand lotion is helpful except for those slather on lotion or the lotion is particularly heavy duty and then they still touch the water. This can cause a double problem - an oil slick which is a terrible section adhesive along with squamous cells from bare skin. Hold slide at top or on sides, as mentioned previously. Wear gloves. Not always popular with a common argument one loses dexterity handling slides. If gloves do not flop around loosely but fit the hand well, then dexterity is not lost. Good luck Gayle Callis HTL/HT/MT(ASCP) **************************************************************************** *************************************************** We have that problem mostly during the winter months when our hands get dry. Use hand lotion, that usually helps a bit. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 -----Original Message----- From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto: histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, October 28, 2014 3:55 PM To: histonet <@t> lists.utsouthwestern.edu Subject: [Histonet] Squamous cells staining on H&E and IHC Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's? I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting? Change out water bath several times during shifts? Any suggestions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Wed Oct 29 19:15:24 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Oct 29 19:15:45 2014 Subject: [Histonet] RE: Squamous cells staining on H&E and IHC In-Reply-To: References: <6ED9D4252F278841A0593D3D788AF24C2760C836@mailsvr.MARSHMED.local> <5A33C952BB67F4468AF1F36D739212BC011255CDAD@JERRY.Gia.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53BA443@xmdb02.nch.kids> Would you believe that according to a few very imminent histotechnologists, the source of nearly all of the squames contaminating slides is dandruff (a little embarrassing!!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor Sent: Wednesday, 29 October 2014 9:44 PM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Squamous cells staining on H&E and IHC When the sections are cut and taken on the slide from water bath, slide must only holds on the frosted end of the slides don't touch other part of slide. Regards Muhammad Tahseen MLT (JIMTEF) Japan Histology Supervisor SKMCH&RC Lahore Pakistan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, October 29, 2014 12:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Squamous cells staining on H&E and IHC Does anyone else have problems with what looks like squamous cells staining on your H&E's and IHC's? I'm trying to figure out how to eliminate that problem in our lab...wear gloves while cutting? Change out water bath several times during shifts? Any suggestions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From j.rowaihi <@t> alborglaboratories.com Thu Oct 30 00:07:26 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Thu Oct 30 00:07:35 2014 Subject: [Histonet] RE: Cap ER/PR In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B278D@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B278D@ex07.net.ucsf.edu> Message-ID: <019101cff3ff$6720da10$35628e30$@rowaihi@alborglaboratories.com> I agree with Tim Morken Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, October 29, 2014 8:15 PM To: 'Heckford, Karen - SMMC-SF'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cap ER/PR Karen, when this has happened in the past CAP has told me to run the test and score it, then when the survey summary comes with the scores and discussion, to have the pathologist review and score the answers and document the review. It's about the best you can do when something gets missed. You may want to have two reviewers sign off in this case. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cap ER/PR Good Morning, Apparently while I was out on disability a CAP ER/PR PM2-A 2014 did not get done. Somehow one of our pathologists got it and forgot she had it. That being said, does anyone have a equivalent test they do instead. We only do this CAP testing to show competency. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Thu Oct 30 07:53:10 2014 From: mw <@t> personifysearch.com (Matt Ward) Date: Thu Oct 30 07:53:18 2014 Subject: [Histonet] Histology Technical Specialist - Toronto Message-ID: <120301cff440$76c59be0$6450d3a0$@personifysearch.com> Good morning, I hope everyone is doing great! We wanted to reach out regarding a new Histology Field Technical Specialist with a world leader in cancer diagnostics. The position will ideally be based in Toronto, and will provide histology technical expertise to internal and external clients in Canada. The ideal candidate will have a background in histology and will be open to travel. Please e-mail directly at mw@personifysearch.com to learn more. Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com From akbitting <@t> geisinger.edu Thu Oct 30 09:37:56 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Oct 30 09:38:03 2014 Subject: [Histonet] RE: QIHC In-Reply-To: References: Message-ID: <77F52EFAB8B1694B885E277C48FCD0F67BA94BDD@GHSEXMBX1W8K1V.geisinger.edu> Yes, it's part of the requirements to be able to take the exam. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Wednesday, October 29, 2014 12:19 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] FW: QIHC From: Amy Johnson Sent: Wednesday, October 29, 2014 6:57 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: QIHC Do you need to have experience with ISH to take the QIHC test? Thanks, Amylin Johnson, BS HTL(ASCP) Associates in Pathology Wausau, WI 715-847-2130 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From AGleiberman <@t> buffalobiolabs.com Thu Oct 30 10:44:17 2014 From: AGleiberman <@t> buffalobiolabs.com (Anatoli Gleiberman) Date: Thu Oct 30 10:44:29 2014 Subject: [Histonet] RE: slides for IHC's In-Reply-To: <7ab8364572044fa0a27e5640d7ac71cc@BL2PR04MB195.namprd04.prod.outlook.com> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED6D6C@vap1014.win.rwjuh.edu> <7ab8364572044fa0a27e5640d7ac71cc@BL2PR04MB195.namprd04.prod.outlook.com> Message-ID: Histobond adhesive slides from StatLab for H&E and IF Anatoli Gleiberman, Ph.D. Director of Histopathology Buffalo Biolabs LLC 73 High Street Buffalo, NY 14203 Phone: 716-849-6810x354 e-mail: agleiberman@buffalobiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark Sent: Wednesday, October 29, 2014 4:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's We also use Leica Apex slides. Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, October 29, 2014 2:38 PM To: 'Goins, Tresa'; Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Leica Apex slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, October 29, 2014 2:53 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Mercedes Medical Starfrost adhesive for IHC and HE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AJohnson <@t> aipathology.com Thu Oct 30 11:37:53 2014 From: AJohnson <@t> aipathology.com (Amy Johnson) Date: Thu Oct 30 11:38:05 2014 Subject: [Histonet] RE: slides for IHC's In-Reply-To: References: Message-ID: What type of instrumentation does everyone use? We use Ventana Benchmark Ultra and they want us to use Superfrost Plus slides only.......curious if other have the same restrictions -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, October 29, 2014 1:53 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Mercedes Medical Starfrost adhesive for IHC and HE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne <@t> rwjuh.edu Thu Oct 30 11:45:35 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Thu Oct 30 11:45:45 2014 Subject: [Histonet] RE: slides for IHC's In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED73F0@vap1014.win.rwjuh.edu> We have a Bond III, and although we use the Apex slides from Leica, we are not required to do so. We have 3 different manufacturer's slides validated should the Apex slides go on backorder. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Thursday, October 30, 2014 12:38 PM To: 'Goins, Tresa'; Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's What type of instrumentation does everyone use? We use Ventana Benchmark Ultra and they want us to use Superfrost Plus slides only.......curious if other have the same restrictions -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, October 29, 2014 1:53 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Mercedes Medical Starfrost adhesive for IHC and HE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mroark <@t> sfmc.net Thu Oct 30 12:12:57 2014 From: mroark <@t> sfmc.net (Matthew D. Roark) Date: Thu Oct 30 12:13:12 2014 Subject: [Histonet] RE: slides for IHC's In-Reply-To: References: Message-ID: <414f807ea3f240e3b59192640b28e5e0@BL2PR04MB195.namprd04.prod.outlook.com> We are using our Leica Apex slides on the Ventana Ultra. No problems. Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Johnson Sent: Thursday, October 30, 2014 11:38 AM To: 'Goins, Tresa'; Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's What type of instrumentation does everyone use? We use Ventana Benchmark Ultra and they want us to use Superfrost Plus slides only.......curious if other have the same restrictions -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, October 29, 2014 1:53 PM To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slides for IHC's Mercedes Medical Starfrost adhesive for IHC and HE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, October 29, 2014 11:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slides for IHC's I would like to know what kind of slides everyone else is using for their IHC's. I am having some problems with tissue falling off the slides during Target Retrieval. This happens mostly on fattier tissue. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Thu Oct 30 12:54:13 2014 From: twheelock <@t> mclean.harvard.edu (Wheelock, Timothy R.) Date: Thu Oct 30 12:54:20 2014 Subject: [Histonet] Tissue Processor Location in the Lab Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9773B3B50@PHSX10MB11.partners.org> Hi Everyone: Even though many tissue processors are closed systems with fume filters, do people try to put them next to fume hoods or under some ventilation duct to add an extra layer of protection from solvent fumes? Thanks, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Timothy.Morken <@t> ucsfmedctr.org Thu Oct 30 13:07:22 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Oct 30 13:09:22 2014 Subject: [Histonet] RE: Tissue Processor Location in the Lab In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC9773B3B50@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC9773B3B50@PHSX10MB11.partners.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367B3F28@ex07.net.ucsf.edu> Tim, We have a separate room for the tissue processors. We have a chemical fume hood for pouring all chemicals into carboys via a funnel that is in a hole cut in the hood work surface, and a carboy directly beneath. We also have a fume extraction system above each tissue processor that is designed to pull any fumes up and away from anyone working at that processor. It does not enclose the processor, but sits above it. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wheelock, Timothy R. Sent: Thursday, October 30, 2014 10:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Processor Location in the Lab Hi Everyone: Even though many tissue processors are closed systems with fume filters, do people try to put them next to fume hoods or under some ventilation duct to add an extra layer of protection from solvent fumes? Thanks, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeathridge <@t> pastnashville.com Thu Oct 30 13:54:11 2014 From: madeathridge <@t> pastnashville.com (Maryann Deathridge) Date: Thu Oct 30 13:55:06 2014 Subject: [Histonet] Solvent Recyclers Message-ID: <1a2e196255914566a1cb46ac5c4b1cd6@pastnashville.com> Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. "Dependability" more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathridge@pastnashville.com From LSebree <@t> uwhealth.org Thu Oct 30 14:12:15 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Oct 30 14:12:23 2014 Subject: [Histonet] Fibronectin Message-ID: <77DD817201982748BC67D7960F2F76AF0E3D5C@UWHC-MBX12.uwhis.hosp.wisc.edu> Does anyone know of a reference lab doing this antibody on human FFPE slides? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From Maxim_71 <@t> mail.ru Thu Oct 30 14:13:42 2014 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Oct 30 14:14:13 2014 Subject: [Histonet] RE: slides for IHC's Message-ID: <565301704.20141030221342@mail.ru> I found that my Leica Apex slides is not suitable for IHC. All the breast FFPE sections fall off after demasking into both FLEX buffers (high and low pH). I uses only Menzel Polysine slides with great success many years for any tissues for IHC. For HE and HC we uses ususal slides with gelatine adhesive. Histonet people's teached me for fatty tissues to use two main rules: 1. Thicknes slices for processing no more than 2 mm 2. Fixation in 10%NBF at least 24-48 hours. Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From GKeyser <@t> uwhealth.org Thu Oct 30 14:28:39 2014 From: GKeyser <@t> uwhealth.org (Keyser Gerald T) Date: Thu Oct 30 14:28:46 2014 Subject: [Histonet] Solvent Recyclers In-Reply-To: <1a2e196255914566a1cb46ac5c4b1cd6@pastnashville.com> References: <1a2e196255914566a1cb46ac5c4b1cd6@pastnashville.com> Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE03A231@UWHC-MBX12.uwhis.hosp.wisc.edu> GRAC http://www.newcomersupply.com/product/gravity-recycling-alcohol-cartridge-tissue-processor-grac-tp I've used this system. It takes quite a while to run. It works. But, there is one big problem. If there is a possibility of Xylene or Propar contamination, it will quickly ruin the filter and cause problems with any system using the recycled ethanol. It doesn't take much to completely mess up the filter. But, it does have the possibility to save a lab quite a bit of money. Gerry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge Sent: Thursday, October 30, 2014 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Solvent Recyclers Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. "Dependability" more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathridge@pastnashville.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Oct 30 14:45:09 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Oct 30 14:45:14 2014 Subject: [Histonet] Solvent Recyclers In-Reply-To: <1a2e196255914566a1cb46ac5c4b1cd6@pastnashville.com> References: <1a2e196255914566a1cb46ac5c4b1cd6@pastnashville.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E391729FF7008@IBMB7Exchange.digestivespecialists.com> CBG http://www.cbgtechnologies.com/ Completely dependable. I've had mine for years with no problems at all. It runs forever! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge Sent: Thursday, October 30, 2014 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Solvent Recyclers Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. "Dependability" more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathridge@pastnashville.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Oct 30 15:05:33 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Oct 30 15:05:43 2014 Subject: [Histonet] Solvent Recyclers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E391729FF7008@IBMB7Exchange.digestivespecialists.com> References: <1a2e196255914566a1cb46ac5c4b1cd6@pastnashville.com> <5A2BD13465E061429D6455C8D6B40E391729FF7008@IBMB7Exchange.digestivespecialists.com> Message-ID: Ditto Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, October 30, 2014 3:45 PM To: madeathridge@pastnashville.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Solvent Recyclers CBG http://www.cbgtechnologies.com/ Completely dependable. I've had mine for years with no problems at all. It runs forever! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge Sent: Thursday, October 30, 2014 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Solvent Recyclers Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. "Dependability" more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathridge@pastnashville.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From mercedesgallagher <@t> gmail.com Thu Oct 30 15:56:02 2014 From: mercedesgallagher <@t> gmail.com (Mercedes Gallagher) Date: Thu Oct 30 15:57:26 2014 Subject: [Histonet] Help! Need someone to repair an IEC CTD Cryostat Message-ID: Hi, I'm having extreme difficulty finding someone who can work on the old IEC CTDs in Southern California. The issue isn't with the microtome, it's with the compressor/refrigeration. I've had a few people tell me they deal with these machines, only to find out after many hours of labor that they don't have a clue. Does anyone have any contacts? Thanks! From patrick.lewis <@t> seattlechildrens.org Thu Oct 30 16:27:14 2014 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Thu Oct 30 16:27:27 2014 Subject: [Histonet] Paraformaldehyde fixation questions Message-ID: <3903BE18914F4440834F0E620415FFCA3CB3D781@PPWEXD01d.childrens.sea.kids> After having a lot of problems using 100% Acetone as a fixative, I am going to try 4% paraformaldehyde in DPBS pH 7.4 for fixation of slides cut from fresh tissues snap frozen in OCT. My plan is to cut the slides at 5 or 6 uM and dry them for 2 hours in the hood, then fix them in the 4% p-f for 10 minutes. Then immediately immerse them in Room temp DPBS. Is 10 minutes fixation long enough? Would 30 minutes work better? Is there any advantage, or disadvantage of using 4C versus Room temp for Paraformaldehyde? I am concerned about the drying of the tissues, and tissue loss from the slide. I'm not sure which will be best, drying after cutting or drying after fixing. Years ago, I vaguely remember being told that you should never dry the tissue after fixing it and then re-wet it. So I am leaning towards drying after cutting, but keeping the slides wet after fixing. The goal is for me to get good fixation, not destroy epitopes, and avoid the tissue damage that I get when I fix with Acetone. Thanks Patrick. PS: My brief internet search stated that Fixation occurs faster at higher temperatures (which I knew) , and that Some antibodies are capricious so for them 4C is better. (= IHC myth that corresponds to find what works and then don't change it) Is it right to assume that 4C is going to be gentler on the tissues than Room temp. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From akbitting <@t> geisinger.edu Thu Oct 30 16:32:17 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Oct 30 16:32:33 2014 Subject: [Histonet] Solvent Recyclers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E391729FF7008@IBMB7Exchange.digestivespecialists.com> References: <1a2e196255914566a1cb46ac5c4b1cd6@pastnashville.com> <5A2BD13465E061429D6455C8D6B40E391729FF7008@IBMB7Exchange.digestivespecialists.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F67BA95B75@GHSEXMBX1W8K1V.geisinger.edu> ditto -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, October 30, 2014 3:45 PM To: madeathridge@pastnashville.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Solvent Recyclers CBG http://www.cbgtechnologies.com/ Completely dependable. I've had mine for years with no problems at all. It runs forever! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryann Deathridge Sent: Thursday, October 30, 2014 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Solvent Recyclers Any recommendations for solvent recyclers? Due to space issues benchtop unit preferred. "Dependability" more preferred. Pros and cons? You may email if preferred. Thanks in advance for responses Maryann . madeathridge@pastnashville.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From latecor <@t> adinet.com.uy Thu Oct 30 17:36:19 2014 From: latecor <@t> adinet.com.uy (Carlos Defeo) Date: Thu Oct 30 17:36:16 2014 Subject: [Histonet] Antibodies for Microsatellite instability Message-ID: <201410302036190151.00130727@adinet.com.uy> hi all, our laboratory in our country,Uruguay, is asked to perform Microsatellite instability on colon and endometrial carcinomas. I need your input on what vendors and clones would be the most reliable for these IHQ tests. Thanks in advance to all who could enlighten me on this point. Carlos defeo histotechnologist From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 31 10:17:16 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Oct 31 10:17:31 2014 Subject: [Histonet] FDA is going to regulate your laboratory Message-ID: <761E2B5697F795489C8710BCC72141FF367B415A@ex07.net.ucsf.edu> BOO! Is there anything scarier than that?!?! Happy Halloween!! And, to rub it in.... The Giants fans were already lining the sidewalks at 5 AM today for today's WORLD SERIES WINNERS PARADE!! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. From hackerlab12 <@t> outlook.com Fri Oct 31 10:25:25 2014 From: hackerlab12 <@t> outlook.com (Lisa Daffern) Date: Fri Oct 31 10:25:38 2014 Subject: [Histonet] Automatic Coverslipping Machine Message-ID: Hacker Instruments & Industries, INC.Automatic Coverslipping MachineVisit Our Website!HCM6000 CoverslipperThe Ultimate CoverslipperThe exclusive mountant media dispensing system eliminates the formation of air bubbles under the coverglass. All new microwaveable slide baskets interface with most automated staining machines.Available with:ready to load, pre-filled cover-glass mountant dispensersCompact footprint requires minimal counterspace.Improve the environment in your lab.STAY IN TOUCH 1132 Kincaid Bridge Road Winnsboro, SC - 803.712.6100? 2014 MicrosoftTermsPrivacy & cookiesDevelopersEnglish (United States) From Richard.Cartun <@t> hhchealth.org Fri Oct 31 11:14:13 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Oct 31 11:14:18 2014 Subject: [Histonet] Storage container for antibody vials Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355E6A90@HHCEXCHMB03.hhcsystem.org> Can someone recommend a storage container for antibody vials that will fit nicely into a refrigerator? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From liz <@t> premierlab.com Fri Oct 31 11:48:51 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Oct 31 11:48:57 2014 Subject: [Histonet] RE: Storage container for antibody vials In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355E6A90@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E355E6A90@HHCEXCHMB03.hhcsystem.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDB15@SBS2K8.premierlab.local> Richard For the antibodies we store in the refrigerator we use the small plastic boxes from the pipette tips for the Dako antibodies. For other vendors we use Eppendorf tube racks or the plastic freezer boxes that are around 5.5" square and contain 100 spaces. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, October 31, 2014 10:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage container for antibody vials Can someone recommend a storage container for antibody vials that will fit nicely into a refrigerator? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Fri Oct 31 12:41:13 2014 From: JWatson <@t> gnf.org (James Watson) Date: Fri Oct 31 12:41:31 2014 Subject: [Histonet] RE: Storage container for antibody vials In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECDB15@SBS2K8.premierlab.local> References: <9215BD4B0BA1B44D962A71C758B68D2E355E6A90@HHCEXCHMB03.hhcsystem.org> <14E2C6176416974295479C64A11CB9AE019C79ECDB15@SBS2K8.premierlab.local> Message-ID: We use the plastic freezer boxes also and have our machine shop take out some of the dividers to store the larger vials in. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, October 31, 2014 9:49 AM To: Cartun, Richard; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Storage container for antibody vials Richard For the antibodies we store in the refrigerator we use the small plastic boxes from the pipette tips for the Dako antibodies. For other vendors we use Eppendorf tube racks or the plastic freezer boxes that are around 5.5" square and contain 100 spaces. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, October 31, 2014 10:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage container for antibody vials Can someone recommend a storage container for antibody vials that will fit nicely into a refrigerator? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pmcdavid <@t> mhg.com Fri Oct 31 12:53:46 2014 From: Pmcdavid <@t> mhg.com (Patti McDavid) Date: Fri Oct 31 12:53:53 2014 Subject: [Histonet] Re: Solvent Recyclers Message-ID: <1F886A89F4A53345928A11203C028957D7AF0641@JANDC600-MBN02.mhg.local> Have had our CBG for a long time. Very reliable. Patti L. McDavid Laboratory Manager 4500 Thirteenth Street P.O. Box 1810 Gulfport, MS 39502-1810 Phone 228-575-2340 Fax 228-865-3325 Pmcdavid@mhg.com [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **********PRIVATE AND CONFIDENTIAL********** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. From michael <@t> mcevoyandfarmer.com Fri Oct 31 12:55:10 2014 From: michael <@t> mcevoyandfarmer.com (Michael Farmer) Date: Fri Oct 31 12:55:20 2014 Subject: [Histonet] Halloween costume advice for the pathology community Message-ID: <27AF04FF-DDCE-4AC8-BDD1-11AB489DB5DF@mcevoyandfarmer.com> Esteemed colleagues - Every year at this time I am besieged by friends and clients asking my advice on what they should dress up as for Halloween. And every October I tell them that the answer varies from year to year, and the most important question that must be answered first is: Whom do you want to scare? If you want to scare Quest or Labcorp, this would be a good year to dress up as Elizabeth Holmes of Theranos. She?s a friendly looking young lass ? Google a picture of her and you?ll see ? so you wouldn?t think she could scare the Scylla and Charybdis of the lab business. But she?s raised $400m and her board is a dream team of advisors to presidents (Kissinger, Schultz, et al). She?s been working in stealth mode for ten years, and is just now rolling out finger-prick sample collection stations in Walgreen?s drug stores, and transparent pricing that?s half the current CMS rates. Vertically integrated too ? they make the instruments, the reagents, and they will control the retail experience as well. Last week I was at the American Society of Human Genetics meeting in San Diego, which must have been a downright terrifying event for they guys who get paid to expand the Qiagen, Abbott Molecular, and Roche Molecular PCR and FISH menus. I counted 37 NGS cancer panels offered by 17 labs, covering anywhere from two (BRCA is so last year) to 1,300 genes. That?s right ? 1,300 ? that Personalis panel ought to be in the Neiman Marcus Christmas catalogue. And all their prices are headed south, fast. So if you want to scare the assay development teams at Abbot Molecular, Roche Molecular, or Qiagen, you should dress up as an Illumina HiSeq 2500 or an Ion Torrent PGM system. If you have a soft spot in your heart for the Qiagen, Roche and Abbott folks, and you feel they are being bullied by Illumina and Ion Torrent, get out your Jonathan Rothberg (the fellow who invented next generation sequencing) costume. Or you could just tape his LinkedIn page to your face and go to the party. https://www.linkedin.com/in/jonathanrothberg. This costume will also work if you want to scare the guys at Bio-Rad who just bought GnuBio. It will also scare Pacific BioSciences shareholders who are feeling giddy about their most recent quarter. Whatever you do, don?t wear this costume around the CEO or CFO of Thermo. Have fun at the Halloween party but at the same time, be merciful and don?t induce any heart attacks. If you want to scare the M&A team at Ventana, Dako, or Leica, you may want to rent a Whole Slide Imaging scanner costume. After the ASHG in San Diego I stopped by the Pathology Visions meeting in San Francisco. The annual digital pathology party I?ve been going to for five years has turned into a wake. You can bet somebody in Basel is rubbing his neck and saying to himself: ?Damn ? I sure could have used that $100m for something that would have driven revenues.? The Leica M&A guys are crying in their beer over $150m. Over at Agilent, they?re breathing a sigh of relief that they managed to dodge that bullet. I really do feel that it is heartless and cruel to pick on innocent, underpaid, exploited histotechs, but if you?re looking for a bunch of people who scare easily, dress up as a Sakura SmartSection or a Kurabo AS-400. I saw these behemoths for the first time in Yokohama this summer at the Japanese annual pathology meeting. These costumes will work for scaring Thermo and Leica core histology product development types as well, but in their complacency they may just roll their eyes and say ?I?ll wait and let that scare me in a couple of years.? Both these instruments are trying to turn embedded tissue into slides, while turning histotechs into burger-flippers. Check in with me next year at this time and I?ll let you know which costumes are hot. Michael Farmer McEvoy & Farmer Pathology 415-994-8852 michael@mcevoyandfarmer.com Those who seek the truth doubt those who find it. - Andr? Gide From lblazek <@t> digestivespecialists.com Fri Oct 31 13:40:26 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Oct 31 13:40:32 2014 Subject: [Histonet] microwave Message-ID: <5A2BD13465E061429D6455C8D6B40E391729FF7285@IBMB7Exchange.digestivespecialists.com> Dear all, Is there anyone out there doing microwave processing of GI biopsies? What are you using and what are the pros and cons of what your choice of instruments has been? Thanks in advance! Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com From tp2 <@t> medicine.wisc.edu Fri Oct 31 14:23:20 2014 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Fri Oct 31 14:23:54 2014 Subject: [Histonet] SIRT1 Message-ID: <54539B58020000DF000126D3@gwmail2.medicine.wisc.edu> Hello, Has anybody out there had any success with directly conjugated SIRT1 antibodies from Novus Biologicals? Thanks, Tom From epeters2 <@t> gmu.edu Fri Oct 31 15:13:33 2014 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Fri Oct 31 15:13:40 2014 Subject: [Histonet] Anyone doing insect histology? Message-ID: <1414786413258.94512@gmu.edu> ?Do any of you do insect histology/histopathology? We are looking for someone to process the insects (primarily bees) and then read the histoslides and provide a report on the findings. Appreciate any assistance you can provide, or potential contacts! Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University Fairfax, VA 22030-4444 From Timothy.Morken <@t> ucsfmedctr.org Fri Oct 31 17:15:49 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Oct 31 17:15:57 2014 Subject: [Histonet] undercounter crushed ice makers? Message-ID: <761E2B5697F795489C8710BCC72141FF367B438A@ex07.net.ucsf.edu> Does anyone use an undercounter crushed ice maker they are happy with? We are looking into this for microtomy ice trays. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. From bcooper <@t> chla.usc.edu Fri Oct 31 17:52:34 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Fri Oct 31 17:52:45 2014 Subject: [Histonet] RE: undercounter crushed ice makers? In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B438A@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B438A@ex07.net.ucsf.edu> Message-ID: We had one at my previous institution. Most of the techs (including me) preferred the large, solid blocks of ice to the crushed stuff, except when it came time to make margaritas. Then we were all over the crushed stuff! The solid blocks hold moisture at the surface better than the crushed ice, and they stay colder longer. Crushed ice doesn't hold a flat surface when you add water to it (even when you compact it), and we seemed to constantly be pushing the blocks down into the slushy abyss. Maybe I didn't give it enough of a try, and I have been accused (rightfully so) of being set in my ways! Not an issue here though--we have a freezer full of big ol' blocks of ice . . . Happy Halloween Histonet!!! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, October 31, 2014 3:16 PM To: Histonet Subject: [Histonet] undercounter crushed ice makers? Does anyone use an undercounter crushed ice maker they are happy with? We are looking into this for microtomy ice trays. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ---------------------------------------------------------------------