[Histonet] Reference to microtome micrometer thickness

[Reuel Cornelia]

Hello Histonetters,
how will we verify the thickness of a paraffin section in a slide. Do
we have a reference regarding  on how to measure the thickness. I based
the  thickness of my section thru the mcirometer on the rotary microtome
but one of our reveiwers does not believed that we are cutting them into
3 micron thickness or 4 micron thickness. Please if we have any
reference, please share it to me. 
 
 
 
 
Reuel Cornelia, BS MT, AMT
Cellular Pathology
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel: 214-559-7766
fax: 214-559-7768