[Histonet] Fast Green

[Jay Lundgren]

     Try 70% ETOH, leave the slides to soak for a good while.  If that
doesn't work after a couple of hours, try 1% HCl in 70% ETOH.

     Potassium permanganate followed by oxalic acid takes out just about
anything, in my experience, but it's pretty harsh (1% aq potassium
permanganate, 2% aq oxalic acid).  Just like cleaning a stain on a shirt,
you always want to start with the weakest solution first, and work your way
up if necessary.

                                        Sincerely,

                                             Jay A. Lundgren, M.S., HTL
(ASCP)

On Fri, Nov 21, 2014 at 12:49 PM, Berger, Rebecca <BergerR1 <@t> email.chop.edu>
wrote:

> Hey there, does anyone have a good method to de-stain Fast green? The
> slides need to be re-stained with H&E.
> Thanks.
>
>
>
>
> Rebecca Berger, HT(ASCP)CM
> Research Technician
> Division of Orthopedic Surgery
> The Children's Hospital of Philadelphia Research Institute
> 3615 Civic Center Boulevard, ARC904 (Lab)
> Philadelphia, PA 19104
> Phone: 267-425-2076  Email: bergerr1 <@t> email.chop.edu<mailto:
> bergerr1 <@t> email.chop.edu>
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