[Histonet] Re: Acetone fixation

[Burton, Lynn]

We use acetone to denature fatty tumors without any problems. They sit in it for at least half an hour. I don't rinse them.


Lynn M Burton
Histology
Animal Disease Lab
Galesburg, Il
309-344-2451



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Saturday, November 15, 2014 2:34 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Acetone fixation

Sheree H (where?) asks:

>>Have questions about using acetone at the grossing counter. Does 
>>anyone
have problems with it being carried over into the formalin on the processor? We rinse, open cassette to let it dry completely and even change the cassette. I've had issues with my processor and want to make sure it's not coming from the acetone.<<

Acetone doesn't belong in the grossing area - too much of a fire and explosion hazard. If your pathologists are using it to fix marking ink onto specimens, they can use 3% acetic acid (half-strength white vinegar). I don't use anything at all - if you blot the specimen dry before you put the ink on, it'll stay put.

Bob Richmond
Samurai Pathologist
Maryville TN
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