[Histonet] RE: Processed blocks before decaling

[Tony Henwood (SCHN)]

I agree

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gerard Spoelstra
Sent: Friday, 14 November 2014 7:29 PM
To: 'Histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Processed blocks before decaling

Hi

I have found that blocks that were insufficiently decalcified, responded best when taken back to formalin left in buffered formalin for 24 hrs and then decalcified. If you take them back to water then decalcify them, decalcification will cause more damage to the tissue then if they spend some more time in formalin. You can also put them through the clean cycle to take them back to water.

Gerard Spoelstra
Veterinary Histology
Murdoch University


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lori.Disher <@t> HCAhealthcare.com
Sent: Friday, 14 November 2014 1:04 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Processed blocks before decaling

Hello,

We had several tissue cassettes put on the processor before they were decaled.   Can I just melt the blocks, run thru xylene, alcohol & water.  Put in decal and re-process?

Thanks in advance for your expert advice!

Lori Disher
Lori.disher <@t> hcahealthcare.com

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