[Histonet] Vantage in Grossing Room
Donna Millard
Donna.Millard <@t> prlnet.com
Fri Nov 7 09:55:02 CST 2014
We currently have Vantage, and are using Vantage for grossing, but are going to move grossing out of Vantage and into CoPath, starting in Vantage at embedding. After re-evaluating grossing workflow, we decided the work required adding and canceling blocks and stains at grossing, going between 2 systems (have to add in CoPath, then manually push to Vantage, and wait for the blocks to cross the interface, then take the container to cassette printer and scan to get new cassettes) was adding too much time and workflow interruption to grossing. The disadvantage is then there isn't total case visibility in Vantage. We observed a bidirectional interface with Dako using CoPath and grossing in CoPath, and really liked it. The entire case is then visible in both systems, and full reports can come out of CoPath. CoPath has told us that the bidirectional does not work as well with Vantage as with other vendors, but I don't know how accurate or to what degree that is--I haven't seen bidirectional in action with Vantage to compare. Whenever we talked "status updates" in the past, we considered only our current CoPath statuses (ordered, printed, verified), but with AB&T those statuses can be defined and can include "grossed" "embedded" "sectioned," a pathologist's initials, filed...though how those statuses work with Vantage bidirectional, I'm not sure. Questions to ask if you consider bidirectional.
You could consider using CoPath AB&T throughout--you would have that capability with the AB&T that you need to run a third party solution, but CoPath labels will not drive Ventana stainers, and that would require CoPath seat licenses for every workstation.
Donna Millard
Director of Anatomic Pathology
Physicians Reference Laboratory, LLC
7800 W. 110th Street,Overland Park, KS 66210
Direct: 913-339-0485
Fax: 913-319-4156
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, November 06, 2014 11:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 132, Issue 7
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Today's Topics:
1. RE: Vantage in Grossing Room (Morken, Timothy)
2. RE: Histonet Digest, Vol 132, Issue 6 (Cary Ward)
3. RE: Vantage in Grossing Room (Michael Mihalik)
4. Leica ASP6025 Processor (Paula Lucas)
5. Placentas (Fortin, Joyce)
6. Placenta's (mtitford <@t> aol.com)
7. RE: Leica ASP6025 Processor (Jamal)
8. RE: Cold plates for icing blocks?
(Sanders, Jeanine (CDC/OID/NCEZID))
9. RE: Cold plates for icing blocks? (Boyd, Debbie M)
10. HISTO/CYTO SKILL REVIEW (rmweber113 <@t> comcast.net)
11. 2 Sucinyl Cystine stain (AKA 2SC) (Sebree Linda A)
12. Tissue falling off positive slides (Pardue, Judith)
13. Re: Tissue falling off positive slides (Patsy Ruegg)
14. HELP (Hans B Snyder)
15. Re: Tissue falling off positive slides (Tony Auge)
16. RE: HISTO/CYTO SKILL REVIEW (Tom McNemar)
17. RE: HELP (Elizabeth Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Wed, 5 Nov 2014 18:31:41 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] Vantage in Grossing Room
To: 'Michael Mihalik' <mike <@t> pathview.com>, 'Histonet'
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<761E2B5697F795489C8710BCC72141FF367B4E60 <@t> ex07.net.ucsf.edu>
Content-Type: text/plain; charset="iso-8859-1"
AB&T is the Copath tracking system but when a third party tracking system is used AB&T is used as the interface to the third party system.
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael Mihalik
Sent: Wednesday, November 05, 2014 9:09 AM
To: Morken, Timothy; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
So AB&T is the actual interface, not their barcode and tracking system?
I understand about the ordering of blocks and stains, etc.
Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: Morken, Timothy [mailto:Timothy.Morken <@t> ucsfmedctr.org]
Sent: Wednesday, November 05, 2014 11:06 AM
To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface).
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA
CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael Mihalik
Sent: Wednesday, November 05, 2014 7:12 AM
To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer
very similar functionality? I'm just curious why you would have both?
As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage.
I hope I'm making sense, but if not, please feel free to ask for clarification.
Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, November 04, 2014 9:49 AM
To: Tom McNemar; 'Morken, Timothy'; Histonet
Subject: [Histonet] Vantage in Grossing Room
For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing?
Thanks!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------
Message: 2
Date: Wed, 5 Nov 2014 13:35:51 -0500
From: "Cary Ward" <cward <@t> cbgbiotech.com>
Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 6
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00b701cff927$53d93ba0$fb8bb2e0$@com>
When he let's us know, is he calling you or me?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: None
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 132, Issue 6
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When replying, please edit your Subject line so it is more specific
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Today's Topics:
1. RE: Animal Tissue/Human Tissue (Anne Murvosh)
2. IHC Validation (Arrington, Karla A)
3. cardiac leaflet processing (Helen Ilsley)
4. RE: IHC Validation (Sebree Linda A)
5. RE: Vantage in Grossing Room (Michael Mihalik)
6. RE: Vantage in Grossing Room (Morken, Timothy)
7. B-5 fixative (Moe, Barbi A)
8. RE: Vantage in Grossing Room (Michael Mihalik)
----------------------------------------------------------------------
Message: 1
Date: Tue, 4 Nov 2014 18:12:45 +0000
From: Anne Murvosh <amurvosh <@t> advancederm.net>
Subject: [Histonet] RE: Animal Tissue/Human Tissue
To: Matthew Lauterbach <Matthew.Lauterbach <@t> avera.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<22BDD9AABC13E24E95D1CF064B75C4B701043B <@t> EX-01.Advancederm.net>
Content-Type: text/plain; charset="us-ascii"
My first job was at a lab that did both human and animal (till a case got
mixed up) the only difference I found was animals were fattier and hairier.
Same microtome. Anne
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matthew
Lauterbach
Sent: Tuesday, November 04, 2014 8:39 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Animal Tissue/Human Tissue
Has anyone used the same microtome to cut animal tissue as well as human
tissue? We are a hospital clinical Histology lab being asked to provide
research study slides from nonhuman sources. Anyone have an input or
precedence? I couldn't find anything that specifically prohibits this
practice from the CAP. Thanks for the help in advance!!
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 2
Date: Wed, 5 Nov 2014 04:26:51 +0000
From: "Arrington, Karla A" <kaarrington <@t> anthc.org>
Subject: [Histonet] IHC Validation
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F362122314DD5C41BD407A39C4FCF8757B9C549C <@t> AKHV-EX2K12MB3.alaska.ihs.gov>
Content-Type: text/plain; charset="us-ascii"
I am validating IHC's. What is the CAP requirements regarding negative
slides. I've asked CAP but have not received a response.
Are 10 negative patient tissues ran for each antibody using the negative
protocol or are they ran using each antibody but also a known
Negative control ran using the negative protocol?
Thanks!
Karla Arrington, HT (ASCP) HIT (AHIMA)
Supervisor of Pathology
ANMC Pathology
kaarrington <@t> anthc.org
------------------------------
Message: 3
Date: Wed, 5 Nov 2014 06:35:54 +0000
From: Helen Ilsley <helen.ilsley <@t> uct.ac.za>
Subject: [Histonet] cardiac leaflet processing
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2CBD60D7-4BA6-4DB4-B34C-6872878FFBD3 <@t> uct.ac.za>
Content-Type: text/plain; charset=WINDOWS-1252
HI
Does anyone have any experience with the processing of cardiac leaflets, I
am finding they tend to curl and make orientation and embedding difficult.
I am now laying them flat between some foam and hoping that this will work
as I am sure it is going into the wax that makes the leaflet curl They look
fine as they come out of the last xylene before entering the wax.
Can one get wax that has a lower melting point than 55C
Any advice would be appreciated.
Tx
Helen Ilsley
Chief Medical Technologist
Cardiovascular Research Unit
UCT
Anzio Road
Observatory
Cape Town
________________________________
UNIVERSITY OF CAPE TOWN
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Message: 4
Date: Wed, 5 Nov 2014 13:36:54 +0000
From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: [Histonet] RE: IHC Validation
To: "'Arrington, Karla A'" <kaarrington <@t> anthc.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77DD817201982748BC67D7960F2F76AF0E560E <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Karla,
We try to run 1/2 of the required cases with known negative cases and the
other 1/2 with known positive cases. In addition, all cases are run with a
negative reagent control in place of the antibody using the optimized
protocol for the antibody. These are your negative reagent controls.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Arrington,
Karla A
Sent: Tuesday, November 04, 2014 10:27 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] IHC Validation
I am validating IHC's. What is the CAP requirements regarding negative
slides. I've asked CAP but have not received a response.
Are 10 negative patient tissues ran for each antibody using the negative
protocol or are they ran using each antibody but also a known Negative
control ran using the negative protocol?
Thanks!
Karla Arrington, HT (ASCP) HIT (AHIMA)
Supervisor of Pathology
ANMC Pathology
kaarrington <@t> anthc.org
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Wed, 5 Nov 2014 10:12:26 -0500
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] Vantage in Grossing Room
To: "'Amber McKenzie'" <amber.mckenzie <@t> gastrodocs.net>, "'Tom
McNemar'" <TMcNemar <@t> lmhealth.org>, "'Morken, Timothy'"
<Timothy.Morken <@t> ucsfmedctr.org>, "'Histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <007601cff90a$e9b49970$bd1dcc50$@pathview.com>
Content-Type: text/plain; charset="iso-8859-1"
Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer
very similar functionality? I'm just curious why you would have both?
As to your question, if you have some validation process occurring at
grossing that requires information from your Copath system, then perhaps you
might want to use Copath at grossing. For instance, if you scan
requisitions or manual drawings or anything of that nature and you require
those scanned images or other information to be verified at grossing, you
might want to use Copath for grossing IF that information is not being sent
across the interface from Copath to Vantage.
I hope Im making sense, but if not, please feel free to ask for
clarification.
Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber
McKenzie
Sent: Tuesday, November 04, 2014 9:49 AM
To: Tom McNemar; 'Morken, Timothy'; Histonet
Subject: [Histonet] Vantage in Grossing Room
For those of you who have Vantage, in your grossing room do you have
Vantage set up or your LIS system? I was thinking it should be vantage, but
our Co-path person said we should have AB&T set up in the grossing room and
let vantage start at embedding, microtomy and case assembly. What are your
thoughts? Do you have a full computer, keyboard, and scanner in grossing?
Thanks!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Wed, 5 Nov 2014 16:05:30 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] Vantage in Grossing Room
To: 'Michael Mihalik' <mike <@t> pathview.com>, 'Amber McKenzie'
<amber.mckenzie <@t> gastrodocs.net>, 'Tom McNemar'
<TMcNemar <@t> lmhealth.org>, 'Histonet'
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<761E2B5697F795489C8710BCC72141FF367B4DE0 <@t> ex07.net.ucsf.edu>
Content-Type: text/plain; charset="iso-8859-1"
Cerner Copath AB&T will not interact in a bi-directional way with a third
party tracking system (except to get "status" updates, ie when a block is
scanned at embedding Vantage will send a message to Copath to update the
block status). That is, you cannot enter a block or stain order in the third
party system and have the data be written back to the Copath database. You
must enter all in Copath and then the third party system picks it up from
there. That is why Copath was telling Anita that they would need to use
Copath alone at grossing (and accessioning, and all stain ordering). Also,
You must buy and install AB&T to use any third party tracking system (AB&T
is the HL7 interface).
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
CONFIDENTIALITY NOTICE: This email message, including any attachments, is
for the sole use of the intended recipient(s) and may contain confidential,
proprietary, and/or privileged information protected by law. If you are not
the intended recipient, you may not use, copy, or distribute this email
message or its attachments. If you believe you have received this email
message in error, please contact the sender by reply email and destroy all
copies of the original message.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
Mihalik
Sent: Wednesday, November 05, 2014 7:12 AM
To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer
very similar functionality? I'm just curious why you would have both?
As to your question, if you have some validation process occurring at
grossing that requires information from your Copath system, then perhaps you
might want to use Copath at grossing. For instance, if you scan
requisitions or manual drawings or anything of that nature and you require
those scanned images or other information to be verified at grossing, you
might want to use Copath for grossing IF that information is not being sent
across the interface from Copath to Vantage.
I hope I'm making sense, but if not, please feel free to ask for
clarification.
Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber
McKenzie
Sent: Tuesday, November 04, 2014 9:49 AM
To: Tom McNemar; 'Morken, Timothy'; Histonet
Subject: [Histonet] Vantage in Grossing Room
For those of you who have Vantage, in your grossing room do you have
Vantage set up or your LIS system? I was thinking it should be vantage, but
our Co-path person said we should have AB&T set up in the grossing room and
let vantage start at embedding, microtomy and case assembly. What are your
thoughts? Do you have a full computer, keyboard, and scanner in grossing?
Thanks!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Wed, 5 Nov 2014 16:53:11 +0000
From: "Moe, Barbi A" <BAMoe <@t> gundersenhealth.org>
Subject: [Histonet] B-5 fixative
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8B4B31D17067E540AC760B4A30190B99014A4055DB <@t> LXEXMB03.gundluth.org>
Content-Type: text/plain; charset="iso-8859-1"
Can anyone tell me why B-5 fixative is called "B-5"?
Does the "B" stand for something, or does the "5" have a meaning?
Any thoughts are greatly appreciated!
Barb Moe
Histology Lab
Gundersen Health System
La Crosse WI
bamoe <@t> gundersenhealth.org<mailto:bamoe <@t> gundersenhealth.org>
------------------------------
Message: 8
Date: Wed, 5 Nov 2014 12:08:43 -0500
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] Vantage in Grossing Room
To: "'Morken, Timothy'" <Timothy.Morken <@t> ucsfmedctr.org>, "'Amber
McKenzie'" <amber.mckenzie <@t> gastrodocs.net>, "'Tom McNemar'"
<TMcNemar <@t> lmhealth.org>, "'Histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <004001cff91b$27f9a8a0$77ecf9e0$@pathview.com>
Content-Type: text/plain; charset="iso-8859-1"
So AB&T is the actual interface, not their barcode and tracking system?
I understand about the ordering of blocks and stains, etc.
Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: Morken, Timothy [mailto:Timothy.Morken <@t> ucsfmedctr.org]
Sent: Wednesday, November 05, 2014 11:06 AM
To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
Cerner Copath AB&T will not interact in a bi-directional way with a third
party tracking system (except to get "status" updates, ie when a block is
scanned at embedding Vantage will send a message to Copath to update the
block status). That is, you cannot enter a block or stain order in the third
party system and have the data be written back to the Copath database. You
must enter all in Copath and then the third party system picks it up from
there. That is why Copath was telling Anita that they would need to use
Copath alone at grossing (and accessioning, and all stain ordering). Also,
You must buy and install AB&T to use any third party tracking system (AB&T
is the HL7 interface).
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center San Francisco, CA
CONFIDENTIALITY NOTICE: This email message, including any attachments, is
for the sole use of the intended recipient(s) and may contain confidential,
proprietary, and/or privileged information protected by law. If you are not
the intended recipient, you may not use, copy, or distribute this email
message or its attachments. If you believe you have received this email
message in error, please contact the sender by reply email and destroy all
copies of the original message.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
Mihalik
Sent: Wednesday, November 05, 2014 7:12 AM
To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer
very similar functionality? I'm just curious why you would have both?
As to your question, if you have some validation process occurring at
grossing that requires information from your Copath system, then perhaps you
might want to use Copath at grossing. For instance, if you scan
requisitions or manual drawings or anything of that nature and you require
those scanned images or other information to be verified at grossing, you
might want to use Copath for grossing IF that information is not being sent
across the interface from Copath to Vantage.
I hope I'm making sense, but if not, please feel free to ask for
clarification.
Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber
McKenzie
Sent: Tuesday, November 04, 2014 9:49 AM
To: Tom McNemar; 'Morken, Timothy'; Histonet
Subject: [Histonet] Vantage in Grossing Room
For those of you who have Vantage, in your grossing room do you have
Vantage set up or your LIS system? I was thinking it should be vantage, but
our Co-path person said we should have AB&T set up in the grossing room and
let vantage start at embedding, microtomy and case assembly. What are your
thoughts? Do you have a full computer, keyboard, and scanner in grossing?
Thanks!
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
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End of Histonet Digest, Vol 132, Issue 6
****************************************
------------------------------
Message: 3
Date: Wed, 5 Nov 2014 13:40:08 -0500
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] Vantage in Grossing Room
To: "'Morken, Timothy'" <Timothy.Morken <@t> ucsfmedctr.org>, "'Histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <007101cff927$ed5f4bc0$c81de340$@pathview.com>
Content-Type: text/plain; charset="iso-8859-1"
I think I got it. Thank you for your explanation and most importantly,
your time.
Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: Morken, Timothy [mailto:Timothy.Morken <@t> ucsfmedctr.org]
Sent: Wednesday, November 05, 2014 1:32 PM
To: 'Michael Mihalik'; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
AB&T is the Copath tracking system but when a third party tracking system is
used AB&T is used as the interface to the third party system.
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center San Francisco, CA
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the intended recipient, you may not use, copy, or distribute this email
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message in error, please contact the sender by reply email and destroy all
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
Mihalik
Sent: Wednesday, November 05, 2014 9:09 AM
To: Morken, Timothy; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
So AB&T is the actual interface, not their barcode and tracking system?
I understand about the ordering of blocks and stains, etc.
Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: Morken, Timothy [mailto:Timothy.Morken <@t> ucsfmedctr.org]
Sent: Wednesday, November 05, 2014 11:06 AM
To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
Cerner Copath AB&T will not interact in a bi-directional way with a third
party tracking system (except to get "status" updates, ie when a block is
scanned at embedding Vantage will send a message to Copath to update the
block status). That is, you cannot enter a block or stain order in the third
party system and have the data be written back to the Copath database. You
must enter all in Copath and then the third party system picks it up from
there. That is why Copath was telling Anita that they would need to use
Copath alone at grossing (and accessioning, and all stain ordering). Also,
You must buy and install AB&T to use any third party tracking system (AB&T
is the HL7 interface).
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center San Francisco, CA
CONFIDENTIALITY NOTICE: This email message, including any attachments, is
for the sole use of the intended recipient(s) and may contain confidential,
proprietary, and/or privileged information protected by law. If you are not
the intended recipient, you may not use, copy, or distribute this email
message or its attachments. If you believe you have received this email
message in error, please contact the sender by reply email and destroy all
copies of the original message.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michael
Mihalik
Sent: Wednesday, November 05, 2014 7:12 AM
To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet'
Subject: RE: [Histonet] Vantage in Grossing Room
Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer
very similar functionality? I'm just curious why you would have both?
As to your question, if you have some validation process occurring at
grossing that requires information from your Copath system, then perhaps you
might want to use Copath at grossing. For instance, if you scan
requisitions or manual drawings or anything of that nature and you require
those scanned images or other information to be verified at grossing, you
might want to use Copath for grossing IF that information is not being sent
across the interface from Copath to Vantage.
I hope I'm making sense, but if not, please feel free to ask for
clarification.
Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber
McKenzie
Sent: Tuesday, November 04, 2014 9:49 AM
To: Tom McNemar; 'Morken, Timothy'; Histonet
Subject: [Histonet] Vantage in Grossing Room
For those of you who have Vantage, in your grossing room do you have
Vantage set up or your LIS system? I was thinking it should be vantage, but
our Co-path person said we should have AB&T set up in the grossing room and
let vantage start at embedding, microtomy and case assembly. What are your
thoughts? Do you have a full computer, keyboard, and scanner in grossing?
Thanks!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Wed, 5 Nov 2014 13:09:56 -0800
From: "Paula Lucas" <plucas <@t> biopath.org>
Subject: [Histonet] Leica ASP6025 Processor
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2EDC1A8E8F12465F8FD0BCCFA0E47F08 <@t> biopath.local>
Content-Type: text/plain; charset="us-ascii"
Hello,
We're considering the Leica ASP6025 processor for our laboratory and I was
hoping I can get pros and cons or any comments such as in performance,
reagent money savings, ease of use, any comment from you.
Thank you so much in advance for taking the time to give me your thoughts,
Paula Lucas
Lab Manager
Bio-Path Medical Group
California
------------------------------
Message: 5
Date: Wed, 5 Nov 2014 16:51:16 -0500
From: "Fortin, Joyce" <Joyce.Fortin <@t> uhsinc.com>
Subject: [Histonet] Placentas
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DC4D8D4743D2384CA1E89568893470265DAA2A5719 <@t> CORP-EXVS03.corp.uhsinc.biz>
Content-Type: text/plain; charset="us-ascii"
Would anyone be willing to share their policy for handling placentas? I have Public Health asking!
Thanks!
UHS of Delaware, Inc. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited, and may be punishable by law. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message.
------------------------------
Message: 6
Date: Wed, 5 Nov 2014 21:05:29 -0500
From: mtitford <@t> aol.com
Subject: [Histonet] Placenta's
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8D1C77D45AE217B-D34-51270 <@t> webmail-va081.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Joyce Fortin asks about placenta's:
I work in a teaching hospital with a busy L&D department and we examine every placenta from every birth. Depending on their history the placenta may be "gross in" (with four blocks) or "gross only". The physicians are happy to have a record of the weight and appearance and dimensions, and microscopic appearance
Other area hospitals, however do not examine their placenta.
Michael Titford
Pathology USA
Mobile AL
------------------------------
Message: 7
Date: Thu, 6 Nov 2014 09:57:22 +0300
From: "Jamal" <j.rowaihi <@t> alborglaboratories.com>
Subject: RE: [Histonet] Leica ASP6025 Processor
To: "'Paula Lucas'" <plucas <@t> biopath.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<002c01cff98e$eb628b90$c227a2b0$@rowaihi <@t> alborglaboratories.com>
Content-Type: text/plain; charset="windows-1256"
Good day Paula Lucas
I have Leica ASP6025 processor since about 6 months.
I am processing about 200 to 250 biopsy cassettes on that machine and I
recommend to have it, it's smart machine, very easy to use and reagent money
savings.
Best Regards,
Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg
Medical Laboratories |? Mobile +966 503629832|
j.rowaihi <@t> alborglaboratories.com
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA |
Phone: +966 12 670 0099 | Fax: +966 12 676 4984 |
www.alborglaboratories.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Lucas
Sent: Thursday, November 06, 2014 12:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica ASP6025 Processor
Hello,
We're considering the Leica ASP6025 processor for our laboratory and I was
hoping I can get pros and cons or any comments such as in performance,
reagent money savings, ease of use, any comment from you.
Thank you so much in advance for taking the time to give me your thoughts,
Paula Lucas
Lab Manager
Bio-Path Medical Group
California
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 6 Nov 2014 13:53:22 +0000
From: "Sanders, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
Subject: [Histonet] RE: Cold plates for icing blocks?
To: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3B2CD438E1628A41BD687E98B963B7811FE445E3 <@t> EMBX-CHAM2.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
We do when we are sectioning blocks that were processed on the TissueTek Xpress as too much water causes poor staining with these.
Jeanine Sanders
CDc Atlanta
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken <@t> ucsfmedctr.org]
Sent: Monday, November 03, 2014 2:29 PM
To: Histonet
Subject: [Histonet] Cold plates for icing blocks?
Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea....
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA
415.514-6042 (office)
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message.
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Thu, 6 Nov 2014 14:11:19 +0000
From: "Boyd, Debbie M" <DKBoyd <@t> chs.net>
Subject: [Histonet] RE: Cold plates for icing blocks?
To: "Sanders, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>, "Morken,
Timothy" <Timothy.Morken <@t> ucsfmedctr.org>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7EAFE982E328304DA6CE2B677BB76246A9EF3A08 <@t> TN001WEXMBX014.US.chs.net>
Content-Type: text/plain; charset="us-ascii"
We use frozen water in Rubbermaid containers. We use the medium size that will last through the cutting of at least 50-60 blocks. As we move blocks off the ice we add uncut blocks. Yes, the ice melts as the morning progresses, but we but gauze on top of the ice to keep the blocks for setting in water. The ice is about an 1 in to 1.5 inches deep so it doesn't melt that fast. Also our room temp is about 67-68 degrees.
Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Sanders, Jeanine (CDC/OID/NCEZID) [jqb7 <@t> cdc.gov]
Sent: Thursday, November 06, 2014 8:53 AM
To: Morken, Timothy; Histonet
Subject: [Histonet] RE: Cold plates for icing blocks?
We do when we are sectioning blocks that were processed on the TissueTek Xpress as too much water causes poor staining with these.
Jeanine Sanders
CDc Atlanta
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken <@t> ucsfmedctr.org]
Sent: Monday, November 03, 2014 2:29 PM
To: Histonet
Subject: [Histonet] Cold plates for icing blocks?
Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea....
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA
415.514-6042 (office)
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
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_______________________________________________
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------------------------------
Message: 10
Date: Thu, 6 Nov 2014 14:56:28 +0000 (UTC)
From: rmweber113 <@t> comcast.net
Subject: [Histonet] HISTO/CYTO SKILL REVIEW
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<616228225.3786732.1415285788476.JavaMail.zimbra <@t> comcast.net>
Content-Type: text/plain; charset=utf-8
Hi,?? I was wondering if anyone knows how often Joint Commission requires cytologist and histologist to have their competency reviewed.?? I'm not talking about their job performance review, but how often to review their job skills with a check list under direct observation.?? We have been doing it quarterly for histologists.
Thanks so much,
Marilynn Weber H.T.(ASCP)QIHC
Coastal Pathology Consulting Services LLC
------------------------------
Message: 11
Date: Thu, 6 Nov 2014 16:00:16 +0000
From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: [Histonet] 2 Sucinyl Cystine stain (AKA 2SC)
To: "'Histonet (Histonet <@t> lists.utsouthwestern.edu)'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77DD817201982748BC67D7960F2F76AF0E58B4 <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Good morning,
Does anyone know of a reference lab offering this IHC stain for human FFPE tissue?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
------------------------------
Message: 12
Date: Thu, 6 Nov 2014 15:38:25 +0000
From: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
Subject: [Histonet] Tissue falling off positive slides
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F8593D93099A864A9730359FA6992A875E6C99F4 <@t> CHIEX016.CHI.catholichealth.net>
Content-Type: text/plain; charset="us-ascii"
WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides.
Judith Pardue
CHI Memorial
Chatt. Tn. 37343
Judith_pardue <@t> memorial.org
This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
------------------------------
Message: 13
Date: Thu, 6 Nov 2014 10:36:25 -0600
From: Patsy Ruegg <pruegghm <@t> hotmail.com>
Subject: Re: [Histonet] Tissue falling off positive slides
To: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY405-EAS212254B80458580EEC5863DD8840 <@t> phx.gbl>
Content-Type: text/plain; charset="us-ascii"
Make sure yr sections r airdryed completely before u bake thm
Sent from my iPhone
> On Nov 6, 2014, at 10:34 AM, "Pardue, Judith" <Judith_Pardue <@t> memorial.org> wrote:
>
> WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides.
>
> Judith Pardue
> CHI Memorial
> Chatt. Tn. 37343
> Judith_pardue <@t> memorial.org
>
> This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Thu, 6 Nov 2014 12:30:41 -0500
From: Hans B Snyder <hans <@t> histologistics.com>
Subject: [Histonet] HELP
To: "histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAAYBjcv0WPXyY-siVW3hXKemMehwg4RPgvvGknxGd-CCEF+-xg <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hello All,
Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14]
(ab2557) for mouse tissue? I have been trying to extinguish the background
staining but cannot find the right combination.
??? We are doing this by hand. ???
The recommended dilution for this antibody is 1:50 with HIER. See used
protocol below.
So far I have tried:
1. concentrations 1:25, 1:50, 1:75, 1:100 - 1:50 gives very high
background but good positive staining, 1:100 gives low background but not
much positive staining.
2. HIER - 3, 5, 10, 20, minutes @ 96C, 20 minutes at 60C all give the
same results - too much back ground
3. We have tried using the H2O2 before the primary and after, increasing
the time from 30 minutes to 45 minutes.
4. Tried incubating in the primary for shorter time (20 minutes) and longer
overnight at 4C.
5. Tried blocking using 3 different serums, first each one then
combinations and longer times.
6. Tried cutting the DAB concentration in 1/2 then 1/3.
We have not tried enzyme or acid epitope retrieval yet.
*Does anyone have a working protocol or suggestions on what to try?*
???Thank you in advance.???
Procedure:
Deparaffinize
1. Heat slides to 60C for 10 minutes (oven).
2. Dry slides at room temp 5 minutes.
3. Xylene 5 minutes.
4. Xylene 5 minutes.
5. 100% ethanol 5 minutes (dehydration).
6. 100% ethanol 2 minutes (dehydration).
7. 95% ethanol for 2 minutes (rehydration).
8. 70% ethanol for 2 minutes (rehydration).
9. Run in DI water for 10 minutes.
Antigen Retrieval
1. Submerge in 0.01M Citrate Buffer (pH 6.0) at 96C for 5 minutes.
2. Cold running water 5 minutes.
Staining
1. Wash in PBS for 1 min.
2. Permeabilize with 0.1% Triton X / 2.5% horse serum + 2.5% fetal
bovine serum for 2 hours.
3. Incubate in primary antibody 30-45 minutes at in 2.5% horse serum
in PBS.
4. Wash 3x in PBS/tween for 5 minutes each.
5. Fresh 3.0% hydrogen peroxide (H2O2) 2x for 20 minutes each.
6. Wash 3x PBS 2 minutes each.
7. Incubate with anti-Rat Ig impress solution (according to vector???s
instructions) 45 minutes.
8. Wash 3x in PBS for 5 minutes each.
9. Prepare Impact DAB substrate (see insert).
10. Using a light microscope, incubate sections with 50-100ul, until
desired darkness occurs (5-30 seconds).
11. Stop DAB reaction using deionized water.
12. Wash in DH2O for 5 minutes
13. Counterstain in Harris hematoxylin for 30 seconds.
14. Wash in DH2O for 5 minutes.
15. Dehydrate 2x in 95% alcohol for 30 seconds each.
16. Dehydrate 3x in 100% alcohol for 2 minutes each.
17. Clear 2x in xylene 5 minutes each.
18. Mount coverslips using Leica mounting media.
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans <@t> histologistics.com
------------------------------
Message: 15
Date: Thu, 6 Nov 2014 10:31:47 -0700
From: Tony Auge <tony.auge <@t> gmail.com>
Subject: Re: [Histonet] Tissue falling off positive slides
To: Patsy Ruegg <pruegghm <@t> hotmail.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Pardue, Judith"
<Judith_Pardue <@t> memorial.org>
Message-ID:
<CAF7UtbhqG-xpfwaj=vm5yTO4VZ0h2L9iOKXBiHhNxH7_-0_MCQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Increase the time and/or temperature. I bake mine at 85 Celsius for 20
minutes on non charged slides. Section thickness is also a factor as well
as the pH of your blueing reagent.
--
Tony Auge HTL (ASCP) QIHC
Histology Supervisor - Chandler Pathology Services
Cell: (651) 373-4768
Email: tony.auge <@t> gmail.com
------------------------------
Message: 16
Date: Thu, 6 Nov 2014 12:40:58 -0500
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: [Histonet] RE: HISTO/CYTO SKILL REVIEW
To: "'rmweber113 <@t> comcast.net'" <rmweber113 <@t> comcast.net>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E9A90E28259D2F4E84308C5E8EA8F7B401A835980D3D <@t> lmhs-exchange>
Content-Type: text/plain; charset="utf-8"
We are JCAHO inspected (just this year) and only do yearly competencies.
Tom Mc Nemar, HT(ASCP)
Histology Supervisor
(740) 348-4163
Licking Memorial Hospital
1320 West Main Street
Newark, OH 43055
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of rmweber113 <@t> comcast.net
Sent: Thursday, November 06, 2014 9:56 AM
To: histonet
Subject: [Histonet] HISTO/CYTO SKILL REVIEW
Hi, I was wondering if anyone knows how often Joint Commission requires cytologist and histologist to have their competency reviewed. I'm not talking about their job performance review, but how often to review their job skills with a check list under direct observation. We have been doing it quarterly for histologists.
Thanks so much,
Marilynn Weber H.T.(ASCP)QIHC
Coastal Pathology Consulting Services LLC
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Message: 17
Date: Thu, 6 Nov 2014 10:41:12 -0700
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: RE: [Histonet] HELP
To: Hans B Snyder <hans <@t> histologistics.com>,
"histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE019C79ECDB7E <@t> SBS2K8.premierlab.local>
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Hans
We use the antibody from Serotec, it works quite nicely. MCA711, proteinase K digestion, rabbit anti-rat secondary and then Envision Rabbit (polymer). We use this antibody at a 1:1200 dilution.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: Thursday, November 06, 2014 10:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HELP
Hello All,
Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14]
(ab2557) for mouse tissue? I have been trying to extinguish the background staining but cannot find the right combination.
??? We are doing this by hand. ???
The recommended dilution for this antibody is 1:50 with HIER. See used
protocol below.
So far I have tried:
1. concentrations 1:25, 1:50, 1:75, 1:100 - 1:50 gives very high background but good positive staining, 1:100 gives low background but not much positive staining.
2. HIER - 3, 5, 10, 20, minutes @ 96C, 20 minutes at 60C all give the same results - too much back ground
3. We have tried using the H2O2 before the primary and after, increasing the time from 30 minutes to 45 minutes.
4. Tried incubating in the primary for shorter time (20 minutes) and longer overnight at 4C.
5. Tried blocking using 3 different serums, first each one then combinations and longer times.
6. Tried cutting the DAB concentration in 1/2 then 1/3.
We have not tried enzyme or acid epitope retrieval yet.
*Does anyone have a working protocol or suggestions on what to try?*
???Thank you in advance.???
Procedure:
Deparaffinize
1. Heat slides to 60C for 10 minutes (oven).
2. Dry slides at room temp 5 minutes.
3. Xylene 5 minutes.
4. Xylene 5 minutes.
5. 100% ethanol 5 minutes (dehydration).
6. 100% ethanol 2 minutes (dehydration).
7. 95% ethanol for 2 minutes (rehydration).
8. 70% ethanol for 2 minutes (rehydration).
9. Run in DI water for 10 minutes.
Antigen Retrieval
1. Submerge in 0.01M Citrate Buffer (pH 6.0) at 96C for 5 minutes.
2. Cold running water 5 minutes.
Staining
1. Wash in PBS for 1 min.
2. Permeabilize with 0.1% Triton X / 2.5% horse serum + 2.5% fetal
bovine serum for 2 hours.
3. Incubate in primary antibody 30-45 minutes at in 2.5% horse serum
in PBS.
4. Wash 3x in PBS/tween for 5 minutes each.
5. Fresh 3.0% hydrogen peroxide (H2O2) 2x for 20 minutes each.
6. Wash 3x PBS 2 minutes each.
7. Incubate with anti-Rat Ig impress solution (according to vector???s
instructions) 45 minutes.
8. Wash 3x in PBS for 5 minutes each.
9. Prepare Impact DAB substrate (see insert).
10. Using a light microscope, incubate sections with 50-100ul, until desired darkness occurs (5-30 seconds).
11. Stop DAB reaction using deionized water.
12. Wash in DH2O for 5 minutes
13. Counterstain in Harris hematoxylin for 30 seconds.
14. Wash in DH2O for 5 minutes.
15. Dehydrate 2x in 95% alcohol for 30 seconds each.
16. Dehydrate 3x in 100% alcohol for 2 minutes each.
17. Clear 2x in xylene 5 minutes each.
18. Mount coverslips using Leica mounting media.
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans <@t> histologistics.com
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End of Histonet Digest, Vol 132, Issue 7
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