[Histonet] Permanent red, Fuchsin and Vector Red not exactly red...

[Elizabeth Chlipala]

Yves

Try Biocares Warp Red, you will still need to air dry the slides prior to coverslipping do not place them in alcohol, air dry, place in xylene and then mount.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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liz <@t> premierlab.com
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yves Heremans
Sent: Thursday, November 06, 2014 1:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red...

Dear Histonetters,

I am trying to combine a peroxidase-DAB staining (brown) with an alkaline phosphatase-staining for detection of two proteins that do not overlap.
I have already tried Dako’s Permanent Red and Fuchsin+ and Vector’s Vector Red as AP substrates but I am not getting a clear red color, rather something brownish which is not very different in color from the DAB reaction product.
Is there anything I can do to obtain a clearer red color ?

Yves
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