[Histonet] Tissue falling off positive slides

[Cooper, Brian]

We bake ours @ 75-80 degrees for 15 minutes in an offline oven, prior to automated staining.  Just a habit I picked up years ago, but I always lean the slide racks diagonally against the side of the oven when they're baking, rather than having them sit entirely upright.  They seem to drain any residual water more completely that way.  We've used Fisher's positive charged slides for years and haven't really had a problem with tissue loss on H&E.  I think your baking temp and time may be a little low and short, respectively. 

Good luck.

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357     Pager: 213-209-0184
bcooper <@t> chla.usc.edu 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Thursday, November 06, 2014 8:36 AM
To: Pardue, Judith
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Tissue falling off positive slides

Make sure yr sections r airdryed completely before u bake thm 

Sent from my iPhone

> On Nov 6, 2014, at 10:34 AM, "Pardue, Judith" <Judith_Pardue <@t> memorial.org> wrote:
> 
> WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides.
> 
> Judith Pardue
> CHI Memorial
> Chatt. Tn. 37343
> Judith_pardue <@t> memorial.org
> 
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