[Histonet] RE: Cold plates for icing blocks?

[Boyd, Debbie M]

We use frozen water in Rubbermaid containers.  We use the medium size that will last through the cutting of at least 50-60 blocks.  As we move blocks off the ice we add uncut blocks.  Yes, the ice melts as the morning progresses, but we but gauze on top of the ice to keep the blocks for setting in water.  The ice is about an 1 in to 1.5 inches deep so it doesn't melt that fast.  Also our room temp is about 67-68 degrees.

Debbie M. Boyd HT (ASCP) | Chief Histologist  | Southside Regional Medical Center | 200 Medical Park Blvd.  |  Petersburg, Va.  23805 | PH 804-765-5025 | FAX 804-765-6058

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From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Sanders, Jeanine (CDC/OID/NCEZID) [jqb7 <@t> cdc.gov]
Sent: Thursday, November 06, 2014 8:53 AM
To: Morken, Timothy; Histonet
Subject: [Histonet] RE: Cold plates for icing blocks?

We do when we are sectioning blocks that were processed on the TissueTek Xpress as too much water causes poor staining with these.

Jeanine Sanders
CDc Atlanta



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From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken <@t> ucsfmedctr.org]
Sent: Monday, November 03, 2014 2:29 PM
To: Histonet
Subject: [Histonet] Cold plates for icing blocks?

Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea....

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>

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