[Histonet] cardiac leaflet processing

[Helen Ilsley]

HI

Does anyone have any experience with the processing of cardiac leaflets, I am finding they tend to curl and make orientation and embedding difficult.
I am now laying them flat between some foam and hoping that  this will work as I am sure it is going into the wax that makes the leaflet curl  They look fine as they come out of the last xylene before entering the wax.
Can one get wax that has a lower melting point than 55C
Any advice would be appreciated.
Tx
Helen Ilsley
Chief Medical Technologist
Cardiovascular Research Unit
UCT
Anzio Road
Observatory
Cape Town



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