From amosbrooks <@t> gmail.com Sun Nov 2 08:40:36 2014 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Nov 2 08:40:42 2014 Subject: [Histonet] Slides for IHC Message-ID: Hi, Part of checking out slides for use should be to take a *blank* slide (right out of the box with no section on it) and look at it under a fluorescent microscope. With increased FISH and fluorescent labelling lately, we have seen many slides that have fluorescent inclusions within the glass of the slide itself. We have had to recut a number of projects because of this. This was using some name brand slides from good sized distributors. It has been seen in a number of different brands as well. Just something else to think about, Amos From jshelley <@t> sanfordburnham.org Mon Nov 3 09:22:01 2014 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Mon Nov 3 09:22:08 2014 Subject: [Histonet] FSH Newsletter Message-ID: Good Morning, I wanted to share with everyone the Florida Society of Histotechnology's Fall newsletter. We have a great article on immunohistochemistry that will allow you to get 1 CEU credit if you submit your answers to our Continuing Education Chairperson. If you are a FSH member it is free and for non-members it will be a $10.00 charge. If you have the desire to submit an article please send your request to our Newsletter Editor and Chairperson. The names and emails to get in touch with the chairpersons are on page 2 of the newsletter. I have attached the link to get to the newsletter quicker, I hope you enjoy!!! http://www.fshgroup.org/wp-content/uploads/2014/11/FSH-Newsletter-October-2014.pdf Kind Regards! John J Shelley 2014-2016 FSH President From ncosenza <@t> siumed.edu Mon Nov 3 09:28:01 2014 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Mon Nov 3 09:28:05 2014 Subject: [Histonet] bone paraffin embedding Message-ID: <54579F01.3070000@siumed.edu> Histonetters: Our lab needs to paraffin embed and cut bone. Is there a special process for fixation of bone, or can it be harvested and dropped right into NBF? -- Nicole Cosenza Research Technician Institute for Plastic Surgery SIU School of Medicine Springfield, Il 217.545.3862 From Terra.Wineman <@t> novusint.com Mon Nov 3 09:37:37 2014 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Mon Nov 3 09:37:50 2014 Subject: [Histonet] bone paraffin embedding In-Reply-To: <54579F01.3070000@siumed.edu> References: <54579F01.3070000@siumed.edu> Message-ID: <1EB8F245A303564EADF12AC7022FA74DCBBC049B@novus-ex01> Yes 1:20 dilution Terra Wineman, HTL (ASCP)CM Research Biologist, Nutritional Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Cosenza Sent: Monday, November 03, 2014 9:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone paraffin embedding Histonetters: Our lab needs to paraffin embed and cut bone. Is there a special process for fixation of bone, or can it be harvested and dropped right into NBF? -- Nicole Cosenza Research Technician Institute for Plastic Surgery SIU School of Medicine Springfield, Il 217.545.3862 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Mon Nov 3 09:56:40 2014 From: mw <@t> personifysearch.com (Matt Ward) Date: Mon Nov 3 09:56:47 2014 Subject: [Histonet] Histology Technical Specialist - New England Message-ID: <064b01cff77e$c2f3a0c0$48dae240$@personifysearch.com> Good morning, We are currently searching for a histology professional to join one of our top clients. The position will be a field based support specialist and will cover New England. The position offers a very competitive salary, bonus, company car, gas card, cell phone, and laptop. The company is requiring the candidate to have a 4 year degree and experience in histology. Please contact me directly to learn more - mw@personifysearch.com . Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com From ratliffjack <@t> hotmail.com Mon Nov 3 11:07:06 2014 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Nov 3 11:07:11 2014 Subject: [Histonet] bone paraffin embedding In-Reply-To: <54579F01.3070000@siumed.edu> References: <54579F01.3070000@siumed.edu> Message-ID: Nicole, You can very easily fix the bone in 10% NBF and then go into your decalcification process. Remember that fixation rate of bone is generally around 1mm per 24 hours (in all directions) and that it is good to have a minimum of 20:1 ratio of solutions to specimen size for each step. I would also recommend either 5% or 10% formic acid for decalcification. Can you tell us more specifics about the bone and what you wish to accomplish histologically? This information would be I might also suggest contacting Sarah Mack (copied to this message) from the University of Rochester. She is the new Hard Tissue Committee Chairperson for the National Society for Histotechnology and an expert in decalcified bone. I might also add that Sarah and Kim Simmons, NSH Education Development Manager, are working on educational opportunities and resource materials for those working with bone, biomaterials and medical device implants. If you are not a member of the NSH then you might want to consider becoming a member www.nsh.org/content/benefits-membership so that you can be kept up to date with what's coming soon from the Hard Tissue Committee. Best Regards, Jack > Date: Mon, 3 Nov 2014 09:28:01 -0600 > From: ncosenza@siumed.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] bone paraffin embedding > > Histonetters: > > Our lab needs to paraffin embed and cut bone. Is there a special > process for fixation of bone, or can it be harvested and dropped right > into NBF? > > -- > Nicole Cosenza > Research Technician > Institute for Plastic Surgery > SIU School of Medicine > Springfield, Il > 217.545.3862 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Mon Nov 3 11:29:13 2014 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Mon Nov 3 11:29:18 2014 Subject: [Histonet] bone paraffin embedding In-Reply-To: References: <54579F01.3070000@siumed.edu>, Message-ID: Sorry Nicole I forgot to finish a thought before I hit send. What I was going to say was any additional information you could provide regarding the specimen and what you wanted to see at the microscope can sometime yield additional information regarding specific processing protocols, staining, tips and tricks, etc. Best, Jack From: ratliffjack@hotmail.com To: ncosenza@siumed.edu; histonet@lists.utsouthwestern.edu CC: sarah_mack@urmc.rochester.edu; kim@nsh.org; ratliffjack@gmail.com Subject: RE: [Histonet] bone paraffin embedding Date: Mon, 3 Nov 2014 12:07:06 -0500 Nicole, You can very easily fix the bone in 10% NBF and then go into your decalcification process. Remember that fixation rate of bone is generally around 1mm per 24 hours (in all directions) and that it is good to have a minimum of 20:1 ratio of solutions to specimen size for each step. I would also recommend either 5% or 10% formic acid for decalcification. Can you tell us more specifics about the bone and what you wish to accomplish histologically? This information would be I might also suggest contacting Sarah Mack (copied to this message) from the University of Rochester. She is the new Hard Tissue Committee Chairperson for the National Society for Histotechnology and an expert in decalcified bone. I might also add that Sarah and Kim Simmons, NSH Education Development Manager, are working on educational opportunities and resource materials for those working with bone, biomaterials and medical device implants. If you are not a member of the NSH then you might want to consider becoming a member www.nsh.org/content/benefits-membership so that you can be kept up to date with what's coming soon from the Hard Tissue Committee. Best Regards, Jack > Date: Mon, 3 Nov 2014 09:28:01 -0600 > From: ncosenza@siumed.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] bone paraffin embedding > > Histonetters: > > Our lab needs to paraffin embed and cut bone. Is there a special > process for fixation of bone, or can it be harvested and dropped right > into NBF? > > -- > Nicole Cosenza > Research Technician > Institute for Plastic Surgery > SIU School of Medicine > Springfield, Il > 217.545.3862 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From VEBain <@t> mdanderson.org Mon Nov 3 11:34:06 2014 From: VEBain <@t> mdanderson.org (Bain,Virginia) Date: Mon Nov 3 11:34:12 2014 Subject: [Histonet] anti-roll plate damage? Message-ID: Greetings, Our lab recently got a new cryostat (a cryostar NX50). It sees light to moderate usage (3 - 20 hours a week) and has been operational for about 3 weeks. It has had a host of problems since we received it (shipped with a busted motor and the sensor in the window did not detect when it was shut which lead to some kind of short and killed the lighting system). I am hesitant to contact the rep again (they?ve told us they?re tired of hearing from us) until I?m certain this is not user error of some sort. For the most part we have been getting nice clean sections. Every so often we start getting lines and tears which continue even when the blade is moved or replaced. Twice now we have resolved this issue by rotating the anti-roll plate to a smooth edge. When we encounter this problem I find that the anti-roll plate is jagged to the touch, which is I think what is causing the tears. I see that some people suggest smoothing the anti-roll plate out on an emory board when it gets rough but this seems very early in the life of the anti-roll plate to me. In my previous lab we used the same anti-roll plate without issue for about 2 years with heavy usage (50-70 hours a week) and so I?m wondering why this anti-roll plate is encountering so much damage? Thanks for the help! -- Virginia Bain Postdoctoral Fellow Richie Lab M D Anderson - Science Park 512-237-6443 From mmsamaan <@t> gmail.com Mon Nov 3 11:53:35 2014 From: mmsamaan <@t> gmail.com (Maria Samaan) Date: Mon Nov 3 11:53:40 2014 Subject: [Histonet] California Society for Histotechnology Meeting May 2014 Message-ID: Greetings, Has anyone received their credits for the California Society for Histotechnology meeting which took place this May 2014, in Woodland Hills. I know a few people who are still missing their credits and some need paperwork for reimbursements. Thanks, Maria Samaan From Sarah_Mack <@t> urmc.rochester.edu Mon Nov 3 12:35:30 2014 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Mon Nov 3 12:35:36 2014 Subject: [Histonet] bone paraffin embedding In-Reply-To: <201411031801.sA3I1WKD019767@pps.reinject> References: <201411031801.sA3I1WKD019767@pps.reinject> Message-ID: Hi Nicole, There are various ways to fix bone. My lab generally fixes mouse hind limbs with 10% Neutral Buffered Formalin for 3 day with or without perfusion. The key to fixation is proper grossing and long enough time for fixative to penetrate tissue. Please feel free to contact me with any other specific questions you may have. Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 ------------------------------ Message: 2 Date: Mon, 03 Nov 2014 09:28:01 -0600 From: Nicole Cosenza Subject: [Histonet] bone paraffin embedding To: "histonet@lists.utsouthwestern.edu" Message-ID: <54579F01.3070000@siumed.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Histonetters: Our lab needs to paraffin embed and cut bone. Is there a special process for fixation of bone, or can it be harvested and dropped right into NBF? -- Nicole Cosenza Research Technician Institute for Plastic Surgery SIU School of Medicine Springfield, Il 217.545.3862 ------------------------------ From Fai.Chung <@t> cshs.org Mon Nov 3 12:50:12 2014 From: Fai.Chung <@t> cshs.org (Chung, Fai) Date: Mon Nov 3 12:50:21 2014 Subject: [Histonet] RE:CSH 2014 In-Reply-To: <41.41.04806.904C7545@espxlsmg03.csmc.edu> Message-ID: I have not receive the certificates for the CSH back in May. I asked several time for the CE because I have many employees that needs it for reimbursement. In our institution, we only have 3 months to submit the documentation so all of us did not get the reimbursement and I don't know if anyone will be encourage to attend next time. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, November 03, 2014 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FSH Newsletter (John Shelley) 2. bone paraffin embedding (Nicole Cosenza) 3. RE: bone paraffin embedding (Wineman, Terra) 4. Histology Technical Specialist - New England (Matt Ward) 5. RE: bone paraffin embedding (Jack Ratliff) 6. RE: bone paraffin embedding (Jack Ratliff) 7. anti-roll plate damage? (Bain,Virginia) 8. California Society for Histotechnology Meeting May 2014 (Maria Samaan) ---------------------------------------------------------------------- Message: 1 Date: Mon, 3 Nov 2014 15:22:01 +0000 From: John Shelley Subject: [Histonet] FSH Newsletter To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning, I wanted to share with everyone the Florida Society of Histotechnology's Fall newsletter. We have a great article on immunohistochemistry that will allow you to get 1 CEU credit if you submit your answers to our Continuing Education Chairperson. If you are a FSH member it is free and for non-members it will be a $10.00 charge. If you have the desire to submit an article please send your request to our Newsletter Editor and Chairperson. The names and emails to get in touch with the chairpersons are on page 2 of the newsletter. I have attached the link to get to the newsletter quicker, I hope you enjoy!!! http://www.fshgroup.org/wp-content/uploads/2014/11/FSH-Newsletter-October-2014.pdf Kind Regards! John J Shelley 2014-2016 FSH President ------------------------------ Message: 2 Date: Mon, 03 Nov 2014 09:28:01 -0600 From: Nicole Cosenza Subject: [Histonet] bone paraffin embedding To: "histonet@lists.utsouthwestern.edu" Message-ID: <54579F01.3070000@siumed.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Histonetters: Our lab needs to paraffin embed and cut bone. Is there a special process for fixation of bone, or can it be harvested and dropped right into NBF? -- Nicole Cosenza Research Technician Institute for Plastic Surgery SIU School of Medicine Springfield, Il 217.545.3862 ------------------------------ Message: 3 Date: Mon, 3 Nov 2014 15:37:37 +0000 From: "Wineman, Terra" Subject: RE: [Histonet] bone paraffin embedding To: Nicole Cosenza , "histonet@lists.utsouthwestern.edu" Message-ID: <1EB8F245A303564EADF12AC7022FA74DCBBC049B@novus-ex01> Content-Type: text/plain; charset="us-ascii" Yes 1:20 dilution Terra Wineman, HTL (ASCP)CM Research Biologist, Nutritional Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Cosenza Sent: Monday, November 03, 2014 9:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone paraffin embedding Histonetters: Our lab needs to paraffin embed and cut bone. Is there a special process for fixation of bone, or can it be harvested and dropped right into NBF? -- Nicole Cosenza Research Technician Institute for Plastic Surgery SIU School of Medicine Springfield, Il 217.545.3862 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 3 Nov 2014 10:56:40 -0500 From: "Matt Ward" Subject: [Histonet] Histology Technical Specialist - New England To: Message-ID: <064b01cff77e$c2f3a0c0$48dae240$@personifysearch.com> Content-Type: text/plain; charset="us-ascii" Good morning, We are currently searching for a histology professional to join one of our top clients. The position will be a field based support specialist and will cover New England. The position offers a very competitive salary, bonus, company car, gas card, cell phone, and laptop. The company is requiring the candidate to have a 4 year degree and experience in histology. Please contact me directly to learn more - mw@personifysearch.com . Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com ------------------------------ Message: 5 Date: Mon, 3 Nov 2014 12:07:06 -0500 From: Jack Ratliff Subject: RE: [Histonet] bone paraffin embedding To: Nicole Cosenza , Histonet Cc: Jack Ratliff , "sarah_mack@urmc.rochester.edu" , "kim@nsh.org" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Nicole, You can very easily fix the bone in 10% NBF and then go into your decalcification process. Remember that fixation rate of bone is generally around 1mm per 24 hours (in all directions) and that it is good to have a minimum of 20:1 ratio of solutions to specimen size for each step. I would also recommend either 5% or 10% formic acid for decalcification. Can you tell us more specifics about the bone and what you wish to accomplish histologically? This information would be I might also suggest contacting Sarah Mack (copied to this message) from the University of Rochester. She is the new Hard Tissue Committee Chairperson for the National Society for Histotechnology and an expert in decalcified bone. I might also add that Sarah and Kim Simmons, NSH Education Development Manager, are working on educational opportunities and resource materials for those working with bone, biomaterials and medical device implants. If you are not a member of the NSH then you might want to consider becoming a member www.nsh.org/content/benefits-membership so that you can be kept up to date with what's coming soon from the Hard Tissue Committee. Best Regards, Jack > Date: Mon, 3 Nov 2014 09:28:01 -0600 > From: ncosenza@siumed.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] bone paraffin embedding > > Histonetters: > > Our lab needs to paraffin embed and cut bone. Is there a special > process for fixation of bone, or can it be harvested and dropped right > into NBF? > > -- > Nicole Cosenza > Research Technician > Institute for Plastic Surgery > SIU School of Medicine > Springfield, Il > 217.545.3862 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 3 Nov 2014 12:29:13 -0500 From: Jack Ratliff Subject: RE: [Histonet] bone paraffin embedding To: Nicole Cosenza , Histonet Cc: Jack Ratliff , "sarah_mack@urmc.rochester.edu" , "kim@nsh.org" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Sorry Nicole I forgot to finish a thought before I hit send. What I was going to say was any additional information you could provide regarding the specimen and what you wanted to see at the microscope can sometime yield additional information regarding specific processing protocols, staining, tips and tricks, etc. Best, Jack From: ratliffjack@hotmail.com To: ncosenza@siumed.edu; histonet@lists.utsouthwestern.edu CC: sarah_mack@urmc.rochester.edu; kim@nsh.org; ratliffjack@gmail.com Subject: RE: [Histonet] bone paraffin embedding Date: Mon, 3 Nov 2014 12:07:06 -0500 Nicole, You can very easily fix the bone in 10% NBF and then go into your decalcification process. Remember that fixation rate of bone is generally around 1mm per 24 hours (in all directions) and that it is good to have a minimum of 20:1 ratio of solutions to specimen size for each step. I would also recommend either 5% or 10% formic acid for decalcification. Can you tell us more specifics about the bone and what you wish to accomplish histologically? This information would be I might also suggest contacting Sarah Mack (copied to this message) from the University of Rochester. She is the new Hard Tissue Committee Chairperson for the National Society for Histotechnology and an expert in decalcified bone. I might also add that Sarah and Kim Simmons, NSH Education Development Manager, are working on educational opportunities and resource materials for those working with bone, biomaterials and medical device implants. If you are not a member of the NSH then you might want to consider becoming a member www.nsh.org/content/benefits-membership so that you can be kept up to date with what's coming soon from the Hard Tissue Committee. Best Regards, Jack > Date: Mon, 3 Nov 2014 09:28:01 -0600 > From: ncosenza@siumed.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] bone paraffin embedding > > Histonetters: > > Our lab needs to paraffin embed and cut bone. Is there a special > process for fixation of bone, or can it be harvested and dropped right > into NBF? > > -- > Nicole Cosenza > Research Technician > Institute for Plastic Surgery > SIU School of Medicine > Springfield, Il > 217.545.3862 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 3 Nov 2014 17:34:06 +0000 From: "Bain,Virginia" Subject: [Histonet] anti-roll plate damage? To: Histonet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Greetings, Our lab recently got a new cryostat (a cryostar NX50). It sees light to moderate usage (3 - 20 hours a week) and has been operational for about 3 weeks. It has had a host of problems since we received it (shipped with a busted motor and the sensor in the window did not detect when it was shut which lead to some kind of short and killed the lighting system). I am hesitant to contact the rep again (they?ve told us they?re tired of hearing from us) until I?m certain this is not user error of some sort. For the most part we have been getting nice clean sections. Every so often we start getting lines and tears which continue even when the blade is moved or replaced. Twice now we have resolved this issue by rotating the anti-roll plate to a smooth edge. When we encounter this problem I find that the anti-roll plate is jagged to the touch, which is I think what is causing the tears. I see that some people suggest smoothing the anti-roll plate out on an emory board when it gets rough but this seems very early in the life of the anti-roll plate to me. In my previous lab we used the same anti-roll plate without issue for about 2 years with heavy usage (50-70 hours a week) and so I?m wondering why this anti-roll plate is encountering so much damage? Thanks for the help! -- Virginia Bain Postdoctoral Fellow Richie Lab M D Anderson - Science Park 512-237-6443 ------------------------------ Message: 8 Date: Mon, 3 Nov 2014 09:53:35 -0800 From: Maria Samaan Subject: [Histonet] California Society for Histotechnology Meeting May 2014 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Greetings, Has anyone received their credits for the California Society for Histotechnology meeting which took place this May 2014, in Woodland Hills. I know a few people who are still missing their credits and some need paperwork for reimbursements. Thanks, Maria Samaan ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 3 **************************************** IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. From Timothy.Morken <@t> ucsfmedctr.org Mon Nov 3 13:29:17 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Nov 3 13:29:25 2014 Subject: [Histonet] Cold plates for icing blocks? Message-ID: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. From TanyaAbbott <@t> catholichealth.net Mon Nov 3 13:31:34 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Nov 3 13:31:41 2014 Subject: [Histonet] Fixation with phenol formalin Message-ID: <852F7D2C14FB464D80E182B15DB138AF394FE140@CHIEX005.CHI.catholichealth.net> We have a brain fixing with phenol formalin, any suggestions on anything special we need to do with the tissue before processing? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Toni.Rathborne <@t> rwjuh.edu Mon Nov 3 13:35:56 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Mon Nov 3 13:36:06 2014 Subject: [Histonet] RE: Cold plates for icing blocks? In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED7F58@vap1014.win.rwjuh.edu> I had an employer years ago who used this. The disadvantage that bothered me the most was the paraffin shavings flying around due to lack of moisture. It created a very "dirty" environment, and there were frequent floaters due to the difficulty in keeping the microtome blade clean. I wouldn't recommend this at all, but maybe your conditions have more humidity and could work with this. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, November 03, 2014 2:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Mon Nov 3 13:43:22 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Nov 3 13:43:27 2014 Subject: [Histonet] RE: Cold plates for icing blocks? In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA320128732CD2@Mail2Node2.ad.uams.edu> Hi Tim, We had looked at one time, for a smaller cold plate to be used at the microtome during sectioning and could not find anything suitable. We were told that they would be very expensive and the one example I found was just that. It would eliminate the water and mess of the melting ice if someone came up a cold plate that would hold say 30 to 40 blocks and could be a controlled temperature. As always we need small to work in Histology!!! Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, November 03, 2014 1:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Joyce.Weems <@t> emoryhealthcare.org Mon Nov 3 14:06:08 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Nov 3 14:06:32 2014 Subject: [Histonet] RE: Cold plates for icing blocks? In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Message-ID: I have an old cold plate from an old embedding center that one of my techs uses. I do wish someone would make a reasonably sized/priced one for individual use. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, November 03, 2014 2:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From HornHV <@t> archildrens.org Mon Nov 3 14:54:28 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Nov 3 14:54:38 2014 Subject: [Histonet] RE: Cold plates for icing blocks? In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Message-ID: <25A4DE08332B19499904459F00AAACB719E55815D1@EVS1.archildrens.org> We are a small lab and we each use a cold plate from the embedding station. Our microtomes sit on either side of the embedding station and each side has a cold plate. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, November 03, 2014 2:06 PM To: 'Morken, Timothy'; Histonet Subject: [Histonet] RE: Cold plates for icing blocks? I have an old cold plate from an old embedding center that one of my techs uses. I do wish someone would make a reasonably sized/priced one for individual use. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, November 03, 2014 2:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From azdudley <@t> hotmail.com Mon Nov 3 15:54:05 2014 From: azdudley <@t> hotmail.com (anita) Date: Mon Nov 3 15:54:09 2014 Subject: [Histonet] histonet workflow Message-ID: How do others divide up the work in the department? Do you have techs on like a weekly schedule to cut , immunos, do gross, log specimens in, change machines? Just wondering how others did it. Thanks Anita Dudley Providence Hospital Mobile alabama From grmarco2 <@t> yahoo.com Mon Nov 3 18:30:30 2014 From: grmarco2 <@t> yahoo.com (Nadine Pizzella) Date: Mon Nov 3 18:30:35 2014 Subject: [Histonet] Histology Technical Specialist - New England In-Reply-To: <064b01cff77e$c2f3a0c0$48dae240$@personifysearch.com> References: <064b01cff77e$c2f3a0c0$48dae240$@personifysearch.com> Message-ID: Hi Matt, This sounds like a great opportunity... Can you please provide more details about the position? I have 20 years of Histology experience and would like to continue to further my career in the field... Thank you, Nadine Pizzella On Nov 3, 2014 10:56 AM, "Matt Ward" wrote: > Good morning, > > > > We are currently searching for a histology professional to join one of our > top clients. The position will be a field based support specialist and will > cover New England. The position offers a very competitive salary, bonus, > company car, gas card, cell phone, and laptop. > > > > The company is requiring the candidate to have a 4 year degree and > experience in histology. > > > > Please contact me directly to learn more - mw@personifysearch.com > . > > > > Thanks! > > Matt > > > > > > Matt Ward > > Program Manager > > Personify > > 5020 Weston Parkway Suite 315 > > Cary NC 27513 > > (Tel) 919.459.3654 > > (Tel) 800.875.6188 direct ext 103 > > (Fax) 919.882.8727 > > www.personifysearch.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nmhisto <@t> comcast.net Mon Nov 3 19:28:26 2014 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Mon Nov 3 19:28:42 2014 Subject: [Histonet] Cold plates for icing blocks? In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Message-ID: <104402193.584633.1415064506740.JavaMail.zimbra@comcast.net> I found a stainless steel pan measuring about 14" long, 9" wide and 5" deep and filled it with DH2O.? I got it out of the freezer when I arrived at work and by the time I was ready to face, there was a bit of melt on top of the ice block that was just right.? Adding a bit more DH20 at the end of the day keeps the surface flat.? My successor puts gauze on the ice for more contact of block surface. I also had two smaller, more shallow stainless steel pans for smaller numbers of blocks.? Restaurant supply stores are a bonanza of ideas for histology but I found the smaller SS pans on eBay.? I inherited the bigger SS pan.? Cheers! From TMcNemar <@t> lmhealth.org Tue Nov 4 04:40:39 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Nov 4 04:40:49 2014 Subject: [Histonet] RE: Cold plates for icing blocks? In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Message-ID: Check out the Histo-cool available from Market Lab or Mercedes Medical (and probably others). We use the small ones at each cutting station and they work great. You can pour a little water in and it stays cold all day. http://www.marketlab.com/histo-cool/p/Histo-Cool/ Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, November 03, 2014 2:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From amber.mckenzie <@t> gastrodocs.net Tue Nov 4 08:48:51 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Nov 4 08:48:53 2014 Subject: [Histonet] Vantage in Grossing Room In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Message-ID: <5A33C952BB67F4468AF1F36D739212BC011255FF50@JERRY.Gia.com> For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! From Neelam.Joshi <@t> nephropath.com Tue Nov 4 08:58:59 2014 From: Neelam.Joshi <@t> nephropath.com (Neelam Joshi) Date: Tue Nov 4 09:04:03 2014 Subject: [Histonet] Protocol for Immunoflrorescence stains on fresh frozen muscle bx Message-ID: Helo Everybody' I am looking for the protocols for doing the immunofluorescence stains on the frozen muscle bx. If anybody can share the protocol it will be helpful. See the attachment please. Thanks Neelam Joshi HT neelam.joshi@nephropath.com From gu.lang <@t> gmx.at Tue Nov 4 10:00:52 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Nov 4 10:01:06 2014 Subject: AW: [Histonet] Cold plates for icing blocks? In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Message-ID: <001b01cff848$8318fb60$894af220$@gmx.at> We use cold-plates. Each microtomy-place has one. it has been working wonderful for the last decades. After an hour switched on "snow" builds up on the plate through room-moisture and as side-effect the blocks get their moisture. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Morken, Timothy Gesendet: Montag, 03. November 2014 20:29 An: Histonet Betreff: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Nov 4 10:29:02 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Nov 4 10:29:09 2014 Subject: [Histonet] RELIA Histology Careers Bulletin 11-04-2014 Exciting opportunities!! RELIA Exclusives!! Hope you had a Happy Halloween! Message-ID: <011001cff84c$723ff1f0$56bfd5d0$@earthlink.net> Hi Histonetters!!! How are you? I hope you had a fun Halloween!! I wanted to drop you a quick line and let you know about some of the positions I am working on. I have a number of really great opportunities For ASCP certified, experienced histologists. All of these positions are full time, permanent and offer excellent pay, great benefits and in some cases sign on bonuses and relocation assistance. The great thing about these opportunities is that if you are ready to move right away they are too and if you want to pursue a position with a start date after the holidays that works as well!! RELIA Histology Spotlight Opportunities: Histology Supervisor & Senior Histotechnologist Needed In Northern AZ These are full time permanent day shift positions in a beautiful state of the art lab. My client offers competitive pay, great benefits and relocation assistance This is a great group of people to work with and a beautiful area to live in! Here is a list of some of our other current openings that I am excited about! Histology Supervisor - Northern AZ Senior Histotechnologist - Northern AZ Lead Histology Tech - St. Louis, MO Histology Technician - Eastern VA Lead Histotechnologist - Belleville, IL Histology/IHC Tech - Eastern TN Histotechnician - Northern CA Histotechnician - East of Chicago Histology Technician - Eastern, TN IHC Supervisor - Dallas, TX Histotechnician - Zanesville, OH CLIA Qual Grossing Histotech - Philadelphia, PA If you or anyone you know might be interested in any of these opportunities or would like help with a job search in another area of the USA please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542 or on my cell phone at 407-353-5070. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Matthew.Lauterbach <@t> avera.org Tue Nov 4 10:38:37 2014 From: Matthew.Lauterbach <@t> avera.org (Matthew Lauterbach) Date: Tue Nov 4 10:39:19 2014 Subject: [Histonet] Animal Tissue/Human Tissue Message-ID: <0FFD9B96579AA64D9A452478AFB414E20B1C3581@AHDC374ML4.averamail.net> Has anyone used the same microtome to cut animal tissue as well as human tissue? We are a hospital clinical Histology lab being asked to provide research study slides from nonhuman sources. Anyone have an input or precedence? I couldn't find anything that specifically prohibits this practice from the CAP. Thanks for the help in advance!! From rjbuesa <@t> yahoo.com Tue Nov 4 10:50:57 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 4 10:51:02 2014 Subject: [Histonet] Animal Tissue/Human Tissue In-Reply-To: <0FFD9B96579AA64D9A452478AFB414E20B1C3581@AHDC374ML4.averamail.net> References: <0FFD9B96579AA64D9A452478AFB414E20B1C3581@AHDC374ML4.averamail.net> Message-ID: <1487317462.454819.1415119857400.JavaMail.yahoo@jws10074.mail.ne1.yahoo.com> If the tissue is correctly paraffin infiltrated?the type of microtome is absolutely irrelevant. Ren? J. On Tuesday, November 4, 2014 11:38 AM, Matthew Lauterbach wrote: Has anyone used the same microtome to cut animal tissue as well as human tissue?? We are a hospital clinical Histology lab being asked to provide research study slides from nonhuman sources.? Anyone have an input or precedence?? I couldn't find anything that specifically prohibits this practice from the CAP.? Thanks for the help in advance!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Tue Nov 4 10:53:18 2014 From: suetp918 <@t> comcast.net (Sue) Date: Tue Nov 4 10:53:32 2014 Subject: [Histonet] histonet workflow In-Reply-To: References: Message-ID: <850176607.4272837.1415119998898.JavaMail.root@comcast.net> We have a weekly schedule prepared monthly Tech 1 rapids, special stains, IHC microtomy Tech 2 bone marrow, cell blocks and assist tech 1, this tech can be pulled for routines if possible Tech 3 biopsies, H&E stainer,?and case distribution Tech 4 our off site?campus work, this tech also?does microtomy on?routine cases?for the main campus Tech 5 embedding, instrumentation (processors), and microtomy, this tech does not get biopsies on the day instrumentsprocessors are broken down Techs 6, 7, 8, 9 embedding?and?microtomy- once cutting complete these techs are assiged other duties ? Of course nothing is in stone there are vacations and calls outs so we must be very flexible. ? S.Paturzo TJUH From PREISZNE <@t> mail.etsu.edu Tue Nov 4 11:05:56 2014 From: PREISZNE <@t> mail.etsu.edu (Preiszner, Johanna) Date: Tue Nov 4 11:06:08 2014 Subject: [Histonet] synuclein control needed Message-ID: Hi, anybody heard about a place where I can buy human alpha-synuclein IHC positive control slides? Or anybody can spare some? Or brains with Lewy bodies? Please? I can offer other control blocks you might need. Thanks, Hanna Preiszner ETSU/QCOM From tp2 <@t> medicine.wisc.edu Tue Nov 4 11:15:07 2014 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Nov 4 11:15:23 2014 Subject: [Histonet] SIRT1 Message-ID: <5458B53B020000DF000127DD@gwmail2.medicine.wisc.edu> For that matter, does anybody have a SIRT1 that works very well for them? It doesn't have to be directly conjugated. Thanks, Tom >>> "Thomas Pier" 10/31/14 2:29 PM >>> Hello, Has anybody out there had any success with directly conjugated SIRT1 antibodies from Novus Biologicals? Thanks, Tom From kalschev <@t> svm.vetmed.wisc.edu Tue Nov 4 11:28:01 2014 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Tue Nov 4 11:28:09 2014 Subject: [Histonet] Nichole - bone processing Message-ID: <54590CA1.1090103@svm.vetmed.wisc.edu> I have experience with bone from all species. Feel free to contact me. Also, check the histonet archives for earlier conversation/hints. Vicki -- Vicki L. Kalscheur School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 2015 Linden Drive Madison, WI 53706-1100 Phone: 608-262-8534 kalschev@vetmed.wisc.edu From amurvosh <@t> advancederm.net Tue Nov 4 12:12:45 2014 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Tue Nov 4 12:12:51 2014 Subject: [Histonet] RE: Animal Tissue/Human Tissue In-Reply-To: <0FFD9B96579AA64D9A452478AFB414E20B1C3581@AHDC374ML4.averamail.net> References: <0FFD9B96579AA64D9A452478AFB414E20B1C3581@AHDC374ML4.averamail.net> Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B701043B@EX-01.Advancederm.net> My first job was at a lab that did both human and animal (till a case got mixed up) the only difference I found was animals were fattier and hairier. Same microtome. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lauterbach Sent: Tuesday, November 04, 2014 8:39 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Animal Tissue/Human Tissue Has anyone used the same microtome to cut animal tissue as well as human tissue? We are a hospital clinical Histology lab being asked to provide research study slides from nonhuman sources. Anyone have an input or precedence? I couldn't find anything that specifically prohibits this practice from the CAP. Thanks for the help in advance!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kaarrington <@t> anthc.org Tue Nov 4 22:26:51 2014 From: kaarrington <@t> anthc.org (Arrington, Karla A) Date: Tue Nov 4 22:27:00 2014 Subject: [Histonet] IHC Validation Message-ID: I am validating IHC's. What is the CAP requirements regarding negative slides. I've asked CAP but have not received a response. Are 10 negative patient tissues ran for each antibody using the negative protocol or are they ran using each antibody but also a known Negative control ran using the negative protocol? Thanks! Karla Arrington, HT (ASCP) HIT (AHIMA) Supervisor of Pathology ANMC Pathology kaarrington@anthc.org From helen.ilsley <@t> uct.ac.za Wed Nov 5 00:35:54 2014 From: helen.ilsley <@t> uct.ac.za (Helen Ilsley) Date: Wed Nov 5 00:36:45 2014 Subject: [Histonet] cardiac leaflet processing Message-ID: <2CBD60D7-4BA6-4DB4-B34C-6872878FFBD3@uct.ac.za> HI Does anyone have any experience with the processing of cardiac leaflets, I am finding they tend to curl and make orientation and embedding difficult. I am now laying them flat between some foam and hoping that this will work as I am sure it is going into the wax that makes the leaflet curl They look fine as they come out of the last xylene before entering the wax. Can one get wax that has a lower melting point than 55C Any advice would be appreciated. Tx Helen Ilsley Chief Medical Technologist Cardiovascular Research Unit UCT Anzio Road Observatory Cape Town ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. From LSebree <@t> uwhealth.org Wed Nov 5 07:36:54 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Nov 5 07:37:00 2014 Subject: [Histonet] RE: IHC Validation In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF0E560E@UWHC-MBX12.uwhis.hosp.wisc.edu> Karla, We try to run 1/2 of the required cases with known negative cases and the other 1/2 with known positive cases. In addition, all cases are run with a negative reagent control in place of the antibody using the optimized protocol for the antibody. These are your negative reagent controls. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A Sent: Tuesday, November 04, 2014 10:27 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] IHC Validation I am validating IHC's. What is the CAP requirements regarding negative slides. I've asked CAP but have not received a response. Are 10 negative patient tissues ran for each antibody using the negative protocol or are they ran using each antibody but also a known Negative control ran using the negative protocol? Thanks! Karla Arrington, HT (ASCP) HIT (AHIMA) Supervisor of Pathology ANMC Pathology kaarrington@anthc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Wed Nov 5 09:12:26 2014 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Nov 5 09:13:06 2014 Subject: [Histonet] Vantage in Grossing Room In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011255FF50@JERRY.Gia.com> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> <5A33C952BB67F4468AF1F36D739212BC011255FF50@JERRY.Gia.com> Message-ID: <007601cff90a$e9b49970$bd1dcc50$@pathview.com> Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I?m making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 5 10:05:30 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Nov 5 10:42:37 2014 Subject: [Histonet] Vantage in Grossing Room In-Reply-To: <007601cff90a$e9b49970$bd1dcc50$@pathview.com> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> <5A33C952BB67F4468AF1F36D739212BC011255FF50@JERRY.Gia.com> <007601cff90a$e9b49970$bd1dcc50$@pathview.com> Message-ID: <761E2B5697F795489C8710BCC72141FF367B4DE0@ex07.net.ucsf.edu> Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BAMoe <@t> gundersenhealth.org Wed Nov 5 10:53:11 2014 From: BAMoe <@t> gundersenhealth.org (Moe, Barbi A) Date: Wed Nov 5 10:53:19 2014 Subject: [Histonet] B-5 fixative Message-ID: <8B4B31D17067E540AC760B4A30190B99014A4055DB@LXEXMB03.gundluth.org> Can anyone tell me why B-5 fixative is called "B-5"? Does the "B" stand for something, or does the "5" have a meaning? Any thoughts are greatly appreciated! Barb Moe Histology Lab Gundersen Health System La Crosse WI bamoe@gundersenhealth.org From mike <@t> pathview.com Wed Nov 5 11:08:43 2014 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Nov 5 11:08:53 2014 Subject: [Histonet] Vantage in Grossing Room In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B4DE0@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> <5A33C952BB67F4468AF1F36D739212BC011255FF50@JERRY.Gia.com> <007601cff90a$e9b49970$bd1dcc50$@pathview.com> <761E2B5697F795489C8710BCC72141FF367B4DE0@ex07.net.ucsf.edu> Message-ID: <004001cff91b$27f9a8a0$77ecf9e0$@pathview.com> So AB&T is the actual interface, not their barcode and tracking system? I understand about the ordering of blocks and stains, etc. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 05, 2014 11:06 AM To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Nov 5 12:31:41 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Nov 5 12:32:16 2014 Subject: [Histonet] Vantage in Grossing Room In-Reply-To: <004001cff91b$27f9a8a0$77ecf9e0$@pathview.com> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> <5A33C952BB67F4468AF1F36D739212BC011255FF50@JERRY.Gia.com> <007601cff90a$e9b49970$bd1dcc50$@pathview.com> <761E2B5697F795489C8710BCC72141FF367B4DE0@ex07.net.ucsf.edu> <004001cff91b$27f9a8a0$77ecf9e0$@pathview.com> Message-ID: <761E2B5697F795489C8710BCC72141FF367B4E60@ex07.net.ucsf.edu> AB&T is the Copath tracking system but when a third party tracking system is used AB&T is used as the interface to the third party system. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 9:09 AM To: Morken, Timothy; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room So AB&T is the actual interface, not their barcode and tracking system? I understand about the ordering of blocks and stains, etc. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 05, 2014 11:06 AM To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cward <@t> cbgbiotech.com Wed Nov 5 12:35:51 2014 From: cward <@t> cbgbiotech.com (Cary Ward) Date: Wed Nov 5 12:38:32 2014 Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 6 In-Reply-To: References: Message-ID: <00b701cff927$53d93ba0$fb8bb2e0$@com> When he let's us know, is he calling you or me? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Animal Tissue/Human Tissue (Anne Murvosh) 2. IHC Validation (Arrington, Karla A) 3. cardiac leaflet processing (Helen Ilsley) 4. RE: IHC Validation (Sebree Linda A) 5. RE: Vantage in Grossing Room (Michael Mihalik) 6. RE: Vantage in Grossing Room (Morken, Timothy) 7. B-5 fixative (Moe, Barbi A) 8. RE: Vantage in Grossing Room (Michael Mihalik) ---------------------------------------------------------------------- Message: 1 Date: Tue, 4 Nov 2014 18:12:45 +0000 From: Anne Murvosh Subject: [Histonet] RE: Animal Tissue/Human Tissue To: Matthew Lauterbach , "'histonet@lists.utsouthwestern.edu'" Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B701043B@EX-01.Advancederm.net> Content-Type: text/plain; charset="us-ascii" My first job was at a lab that did both human and animal (till a case got mixed up) the only difference I found was animals were fattier and hairier. Same microtome. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lauterbach Sent: Tuesday, November 04, 2014 8:39 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Animal Tissue/Human Tissue Has anyone used the same microtome to cut animal tissue as well as human tissue? We are a hospital clinical Histology lab being asked to provide research study slides from nonhuman sources. Anyone have an input or precedence? I couldn't find anything that specifically prohibits this practice from the CAP. Thanks for the help in advance!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 5 Nov 2014 04:26:51 +0000 From: "Arrington, Karla A" Subject: [Histonet] IHC Validation To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I am validating IHC's. What is the CAP requirements regarding negative slides. I've asked CAP but have not received a response. Are 10 negative patient tissues ran for each antibody using the negative protocol or are they ran using each antibody but also a known Negative control ran using the negative protocol? Thanks! Karla Arrington, HT (ASCP) HIT (AHIMA) Supervisor of Pathology ANMC Pathology kaarrington@anthc.org ------------------------------ Message: 3 Date: Wed, 5 Nov 2014 06:35:54 +0000 From: Helen Ilsley Subject: [Histonet] cardiac leaflet processing To: "histonet@lists.utsouthwestern.edu" Message-ID: <2CBD60D7-4BA6-4DB4-B34C-6872878FFBD3@uct.ac.za> Content-Type: text/plain; charset=WINDOWS-1252 HI Does anyone have any experience with the processing of cardiac leaflets, I am finding they tend to curl and make orientation and embedding difficult. I am now laying them flat between some foam and hoping that this will work as I am sure it is going into the wax that makes the leaflet curl They look fine as they come out of the last xylene before entering the wax. Can one get wax that has a lower melting point than 55C Any advice would be appreciated. Tx Helen Ilsley Chief Medical Technologist Cardiovascular Research Unit UCT Anzio Road Observatory Cape Town ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ------------------------------ Message: 4 Date: Wed, 5 Nov 2014 13:36:54 +0000 From: Sebree Linda A Subject: [Histonet] RE: IHC Validation To: "'Arrington, Karla A'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <77DD817201982748BC67D7960F2F76AF0E560E@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Karla, We try to run 1/2 of the required cases with known negative cases and the other 1/2 with known positive cases. In addition, all cases are run with a negative reagent control in place of the antibody using the optimized protocol for the antibody. These are your negative reagent controls. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A Sent: Tuesday, November 04, 2014 10:27 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] IHC Validation I am validating IHC's. What is the CAP requirements regarding negative slides. I've asked CAP but have not received a response. Are 10 negative patient tissues ran for each antibody using the negative protocol or are they ran using each antibody but also a known Negative control ran using the negative protocol? Thanks! Karla Arrington, HT (ASCP) HIT (AHIMA) Supervisor of Pathology ANMC Pathology kaarrington@anthc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 5 Nov 2014 10:12:26 -0500 From: "Michael Mihalik" Subject: RE: [Histonet] Vantage in Grossing Room To: "'Amber McKenzie'" , "'Tom McNemar'" , "'Morken, Timothy'" , "'Histonet'" Message-ID: <007601cff90a$e9b49970$bd1dcc50$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope Im making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 5 Nov 2014 16:05:30 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] Vantage in Grossing Room To: 'Michael Mihalik' , 'Amber McKenzie' , 'Tom McNemar' , 'Histonet' Message-ID: <761E2B5697F795489C8710BCC72141FF367B4DE0@ex07.net.ucsf.edu> Content-Type: text/plain; charset="iso-8859-1" Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 5 Nov 2014 16:53:11 +0000 From: "Moe, Barbi A" Subject: [Histonet] B-5 fixative To: "histonet@lists.utsouthwestern.edu" Message-ID: <8B4B31D17067E540AC760B4A30190B99014A4055DB@LXEXMB03.gundluth.org> Content-Type: text/plain; charset="iso-8859-1" Can anyone tell me why B-5 fixative is called "B-5"? Does the "B" stand for something, or does the "5" have a meaning? Any thoughts are greatly appreciated! Barb Moe Histology Lab Gundersen Health System La Crosse WI bamoe@gundersenhealth.org ------------------------------ Message: 8 Date: Wed, 5 Nov 2014 12:08:43 -0500 From: "Michael Mihalik" Subject: RE: [Histonet] Vantage in Grossing Room To: "'Morken, Timothy'" , "'Amber McKenzie'" , "'Tom McNemar'" , "'Histonet'" Message-ID: <004001cff91b$27f9a8a0$77ecf9e0$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" So AB&T is the actual interface, not their barcode and tracking system? I understand about the ordering of blocks and stains, etc. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 05, 2014 11:06 AM To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 6 **************************************** From mike <@t> pathview.com Wed Nov 5 12:40:08 2014 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Nov 5 12:40:17 2014 Subject: [Histonet] Vantage in Grossing Room In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B4E60@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> <5A33C952BB67F4468AF1F36D739212BC011255FF50@JERRY.Gia.com> <007601cff90a$e9b49970$bd1dcc50$@pathview.com> <761E2B5697F795489C8710BCC72141FF367B4DE0@ex07.net.ucsf.edu> <004001cff91b$27f9a8a0$77ecf9e0$@pathview.com> <761E2B5697F795489C8710BCC72141FF367B4E60@ex07.net.ucsf.edu> Message-ID: <007101cff927$ed5f4bc0$c81de340$@pathview.com> I think I got it. Thank you for your explanation and most importantly, your time. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 05, 2014 1:32 PM To: 'Michael Mihalik'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room AB&T is the Copath tracking system but when a third party tracking system is used AB&T is used as the interface to the third party system. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 9:09 AM To: Morken, Timothy; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room So AB&T is the actual interface, not their barcode and tracking system? I understand about the ordering of blocks and stains, etc. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 05, 2014 11:06 AM To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Wed Nov 5 15:09:56 2014 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed Nov 5 15:00:05 2014 Subject: [Histonet] Leica ASP6025 Processor Message-ID: <2EDC1A8E8F12465F8FD0BCCFA0E47F08@biopath.local> Hello, We're considering the Leica ASP6025 processor for our laboratory and I was hoping I can get pros and cons or any comments such as in performance, reagent money savings, ease of use, any comment from you. Thank you so much in advance for taking the time to give me your thoughts, Paula Lucas Lab Manager Bio-Path Medical Group California From Joyce.Fortin <@t> uhsinc.com Wed Nov 5 15:51:16 2014 From: Joyce.Fortin <@t> uhsinc.com (Fortin, Joyce) Date: Wed Nov 5 15:51:21 2014 Subject: [Histonet] Placentas Message-ID: Would anyone be willing to share their policy for handling placentas? I have Public Health asking! Thanks! UHS of Delaware, Inc. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited, and may be punishable by law. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. From mtitford <@t> aol.com Wed Nov 5 20:05:29 2014 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Nov 5 20:05:33 2014 Subject: [Histonet] Placenta's Message-ID: <8D1C77D45AE217B-D34-51270@webmail-va081.sysops.aol.com> Joyce Fortin asks about placenta's: I work in a teaching hospital with a busy L&D department and we examine every placenta from every birth. Depending on their history the placenta may be "gross in" (with four blocks) or "gross only". The physicians are happy to have a record of the weight and appearance and dimensions, and microscopic appearance Other area hospitals, however do not examine their placenta. Michael Titford Pathology USA Mobile AL From j.rowaihi <@t> alborglaboratories.com Thu Nov 6 00:57:22 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Thu Nov 6 00:57:23 2014 Subject: [Histonet] Leica ASP6025 Processor In-Reply-To: <2EDC1A8E8F12465F8FD0BCCFA0E47F08@biopath.local> References: <2EDC1A8E8F12465F8FD0BCCFA0E47F08@biopath.local> Message-ID: <002c01cff98e$eb628b90$c227a2b0$@rowaihi@alborglaboratories.com> Good day Paula Lucas I have Leica ASP6025 processor since about 6 months. I am processing about 200 to 250 biopsy cassettes on that machine and I recommend to have it, it's smart machine, very easy to use and reagent money savings. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, November 06, 2014 12:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica ASP6025 Processor Hello, We're considering the Leica ASP6025 processor for our laboratory and I was hoping I can get pros and cons or any comments such as in performance, reagent money savings, ease of use, any comment from you. Thank you so much in advance for taking the time to give me your thoughts, Paula Lucas Lab Manager Bio-Path Medical Group California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Nov 6 07:53:22 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu Nov 6 07:53:39 2014 Subject: [Histonet] RE: Cold plates for icing blocks? In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu> Message-ID: <3B2CD438E1628A41BD687E98B963B7811FE445E3@EMBX-CHAM2.cdc.gov> We do when we are sectioning blocks that were processed on the TissueTek Xpress as too much water causes poor staining with these. Jeanine Sanders CDc Atlanta ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Monday, November 03, 2014 2:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Thu Nov 6 08:11:19 2014 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Thu Nov 6 08:13:33 2014 Subject: [Histonet] RE: Cold plates for icing blocks? In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FE445E3@EMBX-CHAM2.cdc.gov> References: <761E2B5697F795489C8710BCC72141FF367B4665@ex07.net.ucsf.edu>, <3B2CD438E1628A41BD687E98B963B7811FE445E3@EMBX-CHAM2.cdc.gov> Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9EF3A08@TN001WEXMBX014.US.chs.net> We use frozen water in Rubbermaid containers. We use the medium size that will last through the cutting of at least 50-60 blocks. As we move blocks off the ice we add uncut blocks. Yes, the ice melts as the morning progresses, but we but gauze on top of the ice to keep the blocks for setting in water. The ice is about an 1 in to 1.5 inches deep so it doesn't melt that fast. Also our room temp is about 67-68 degrees. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sanders, Jeanine (CDC/OID/NCEZID) [jqb7@cdc.gov] Sent: Thursday, November 06, 2014 8:53 AM To: Morken, Timothy; Histonet Subject: [Histonet] RE: Cold plates for icing blocks? We do when we are sectioning blocks that were processed on the TissueTek Xpress as too much water causes poor staining with these. Jeanine Sanders CDc Atlanta ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Monday, November 03, 2014 2:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From rmweber113 <@t> comcast.net Thu Nov 6 08:56:28 2014 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Thu Nov 6 08:56:56 2014 Subject: [Histonet] HISTO/CYTO SKILL REVIEW In-Reply-To: <1815966427.3777745.1415285377005.JavaMail.zimbra@comcast.net> Message-ID: <616228225.3786732.1415285788476.JavaMail.zimbra@comcast.net> Hi,? I was wondering if anyone knows how often Joint Commission requires cytologist and histologist to have their competency reviewed.? I'm not talking about their job performance review, but how often to review their job skills with a check list under direct observation.? We have been doing it quarterly for histologists. Thanks so much, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC From LSebree <@t> uwhealth.org Thu Nov 6 10:00:16 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Nov 6 10:00:27 2014 Subject: [Histonet] 2 Sucinyl Cystine stain (AKA 2SC) Message-ID: <77DD817201982748BC67D7960F2F76AF0E58B4@UWHC-MBX12.uwhis.hosp.wisc.edu> Good morning, Does anyone know of a reference lab offering this IHC stain for human FFPE tissue? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From Judith_Pardue <@t> memorial.org Thu Nov 6 09:38:25 2014 From: Judith_Pardue <@t> memorial.org (Pardue, Judith) Date: Thu Nov 6 10:33:10 2014 Subject: [Histonet] Tissue falling off positive slides Message-ID: WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 Judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From pruegghm <@t> hotmail.com Thu Nov 6 10:36:25 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Thu Nov 6 10:36:35 2014 Subject: [Histonet] Tissue falling off positive slides In-Reply-To: References: Message-ID: Make sure yr sections r airdryed completely before u bake thm Sent from my iPhone > On Nov 6, 2014, at 10:34 AM, "Pardue, Judith" wrote: > > WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. > > Judith Pardue > CHI Memorial > Chatt. Tn. 37343 > Judith_pardue@memorial.org > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans <@t> histologistics.com Thu Nov 6 11:30:41 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Nov 6 11:30:46 2014 Subject: [Histonet] HELP Message-ID: Hello All, Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14] (ab2557) for mouse tissue? I have been trying to extinguish the background staining but cannot find the right combination. ? We are doing this by hand. ? The recommended dilution for this antibody is 1:50 with HIER. See used protocol below. So far I have tried: 1. concentrations 1:25, 1:50, 1:75, 1:100 - 1:50 gives very high background but good positive staining, 1:100 gives low background but not much positive staining. 2. HIER - 3, 5, 10, 20, minutes @ 96C, 20 minutes at 60C all give the same results - too much back ground 3. We have tried using the H2O2 before the primary and after, increasing the time from 30 minutes to 45 minutes. 4. Tried incubating in the primary for shorter time (20 minutes) and longer overnight at 4C. 5. Tried blocking using 3 different serums, first each one then combinations and longer times. 6. Tried cutting the DAB concentration in 1/2 then 1/3. We have not tried enzyme or acid epitope retrieval yet. *Does anyone have a working protocol or suggestions on what to try?* ?Thank you in advance.? Procedure: Deparaffinize 1. Heat slides to 60C for 10 minutes (oven). 2. Dry slides at room temp 5 minutes. 3. Xylene 5 minutes. 4. Xylene 5 minutes. 5. 100% ethanol 5 minutes (dehydration). 6. 100% ethanol 2 minutes (dehydration). 7. 95% ethanol for 2 minutes (rehydration). 8. 70% ethanol for 2 minutes (rehydration). 9. Run in DI water for 10 minutes. Antigen Retrieval 1. Submerge in 0.01M Citrate Buffer (pH 6.0) at 96C for 5 minutes. 2. Cold running water 5 minutes. Staining 1. Wash in PBS for 1 min. 2. Permeabilize with 0.1% Triton X / 2.5% horse serum + 2.5% fetal bovine serum for 2 hours. 3. Incubate in primary antibody 30-45 minutes at in 2.5% horse serum in PBS. 4. Wash 3x in PBS/tween for 5 minutes each. 5. Fresh 3.0% hydrogen peroxide (H2O2) 2x for 20 minutes each. 6. Wash 3x PBS 2 minutes each. 7. Incubate with anti-Rat Ig impress solution (according to vector?s instructions) 45 minutes. 8. Wash 3x in PBS for 5 minutes each. 9. Prepare Impact DAB substrate (see insert). 10. Using a light microscope, incubate sections with 50-100ul, until desired darkness occurs (5-30 seconds). 11. Stop DAB reaction using deionized water. 12. Wash in DH2O for 5 minutes 13. Counterstain in Harris hematoxylin for 30 seconds. 14. Wash in DH2O for 5 minutes. 15. Dehydrate 2x in 95% alcohol for 30 seconds each. 16. Dehydrate 3x in 100% alcohol for 2 minutes each. 17. Clear 2x in xylene 5 minutes each. 18. Mount coverslips using Leica mounting media. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From tony.auge <@t> gmail.com Thu Nov 6 11:31:47 2014 From: tony.auge <@t> gmail.com (Tony Auge) Date: Thu Nov 6 11:31:52 2014 Subject: [Histonet] Tissue falling off positive slides In-Reply-To: References: Message-ID: Increase the time and/or temperature. I bake mine at 85 Celsius for 20 minutes on non charged slides. Section thickness is also a factor as well as the pH of your blueing reagent. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com From TMcNemar <@t> lmhealth.org Thu Nov 6 11:40:58 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Nov 6 11:41:14 2014 Subject: [Histonet] RE: HISTO/CYTO SKILL REVIEW In-Reply-To: <616228225.3786732.1415285788476.JavaMail.zimbra@comcast.net> References: <1815966427.3777745.1415285377005.JavaMail.zimbra@comcast.net> <616228225.3786732.1415285788476.JavaMail.zimbra@comcast.net> Message-ID: We are JCAHO inspected (just this year) and only do yearly competencies. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rmweber113@comcast.net Sent: Thursday, November 06, 2014 9:56 AM To: histonet Subject: [Histonet] HISTO/CYTO SKILL REVIEW Hi, I was wondering if anyone knows how often Joint Commission requires cytologist and histologist to have their competency reviewed. I'm not talking about their job performance review, but how often to review their job skills with a check list under direct observation. We have been doing it quarterly for histologists. Thanks so much, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From liz <@t> premierlab.com Thu Nov 6 11:41:12 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Nov 6 11:41:20 2014 Subject: [Histonet] HELP In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDB7E@SBS2K8.premierlab.local> Hans We use the antibody from Serotec, it works quite nicely. MCA711, proteinase K digestion, rabbit anti-rat secondary and then Envision Rabbit (polymer). We use this antibody at a 1:1200 dilution. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: Thursday, November 06, 2014 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP Hello All, Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14] (ab2557) for mouse tissue? I have been trying to extinguish the background staining but cannot find the right combination. ? We are doing this by hand. ? The recommended dilution for this antibody is 1:50 with HIER. See used protocol below. So far I have tried: 1. concentrations 1:25, 1:50, 1:75, 1:100 - 1:50 gives very high background but good positive staining, 1:100 gives low background but not much positive staining. 2. HIER - 3, 5, 10, 20, minutes @ 96C, 20 minutes at 60C all give the same results - too much back ground 3. We have tried using the H2O2 before the primary and after, increasing the time from 30 minutes to 45 minutes. 4. Tried incubating in the primary for shorter time (20 minutes) and longer overnight at 4C. 5. Tried blocking using 3 different serums, first each one then combinations and longer times. 6. Tried cutting the DAB concentration in 1/2 then 1/3. We have not tried enzyme or acid epitope retrieval yet. *Does anyone have a working protocol or suggestions on what to try?* ?Thank you in advance.? Procedure: Deparaffinize 1. Heat slides to 60C for 10 minutes (oven). 2. Dry slides at room temp 5 minutes. 3. Xylene 5 minutes. 4. Xylene 5 minutes. 5. 100% ethanol 5 minutes (dehydration). 6. 100% ethanol 2 minutes (dehydration). 7. 95% ethanol for 2 minutes (rehydration). 8. 70% ethanol for 2 minutes (rehydration). 9. Run in DI water for 10 minutes. Antigen Retrieval 1. Submerge in 0.01M Citrate Buffer (pH 6.0) at 96C for 5 minutes. 2. Cold running water 5 minutes. Staining 1. Wash in PBS for 1 min. 2. Permeabilize with 0.1% Triton X / 2.5% horse serum + 2.5% fetal bovine serum for 2 hours. 3. Incubate in primary antibody 30-45 minutes at in 2.5% horse serum in PBS. 4. Wash 3x in PBS/tween for 5 minutes each. 5. Fresh 3.0% hydrogen peroxide (H2O2) 2x for 20 minutes each. 6. Wash 3x PBS 2 minutes each. 7. Incubate with anti-Rat Ig impress solution (according to vector?s instructions) 45 minutes. 8. Wash 3x in PBS for 5 minutes each. 9. Prepare Impact DAB substrate (see insert). 10. Using a light microscope, incubate sections with 50-100ul, until desired darkness occurs (5-30 seconds). 11. Stop DAB reaction using deionized water. 12. Wash in DH2O for 5 minutes 13. Counterstain in Harris hematoxylin for 30 seconds. 14. Wash in DH2O for 5 minutes. 15. Dehydrate 2x in 95% alcohol for 30 seconds each. 16. Dehydrate 3x in 100% alcohol for 2 minutes each. 17. Clear 2x in xylene 5 minutes each. 18. Mount coverslips using Leica mounting media. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From csegovia <@t> nsalabs.com Thu Nov 6 12:23:40 2014 From: csegovia <@t> nsalabs.com (Segovia, Claudia) Date: Thu Nov 6 12:23:53 2014 Subject: [Histonet] Endogenous Tau (normal Tau) Message-ID: <2A1220D9-2517-4808-A431-107798C6D28E@nsalabs.com> Hi there, I am writing a poster on different Tau epitopes in human brain free floating sections. I have been unable to get any normal Tau and I wonder what could be the problem. Does anybody know what could be? Also, does anyone have a protocol that can share with me? Thank you! Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csegovia@nsalabs.com STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csegovia@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us. From BSullivan <@t> virtua.org Thu Nov 6 12:28:14 2014 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Thu Nov 6 12:28:32 2014 Subject: [Histonet] RE: HISTO/CYTO SKILL REVIEW In-Reply-To: References: <1815966427.3777745.1415285377005.JavaMail.zimbra@comcast.net> <616228225.3786732.1415285788476.JavaMail.zimbra@comcast.net> Message-ID: <6932520047F7EE46B512E9801344F16080040AE5@ExchangeMB-1.Virtua.org> We do a yearly review. We are Joint Commission also. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, November 06, 2014 12:41 PM To: 'rmweber113@comcast.net'; histonet Subject: [Histonet] RE: HISTO/CYTO SKILL REVIEW We are JCAHO inspected (just this year) and only do yearly competencies. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rmweber113@comcast.net Sent: Thursday, November 06, 2014 9:56 AM To: histonet Subject: [Histonet] HISTO/CYTO SKILL REVIEW Hi, I was wondering if anyone knows how often Joint Commission requires cytologist and histologist to have their competency reviewed. I'm not talking about their job performance review, but how often to review their job skills with a check list under direct observation. We have been doing it quarterly for histologists. Thanks so much, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From brett_connolly <@t> merck.com Thu Nov 6 12:29:12 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Nov 6 12:29:19 2014 Subject: [Histonet] HELP In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECDB7E@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECDB7E@SBS2K8.premierlab.local> Message-ID: We also use the MCA711 antibody (at 1.0 ug/mL, 1 hr.), but with HIER in citrate buffer using a Biocare Decloaker pressure cooker and Biocare's rat-on mouse HRP polymer detection. We get very nice staining with no background. I was never too impressed with ImPRESS. Here are the basics : - Deparaffinize and hydrate to dH20 - Perform HIER, cool for 20 min then wash in H20 - 3.0% H2O2 - 20 min - Serum free block (Biocare Sniper) 30 min - Incubate with MCA771 1 hr. - Incubate with Biocare rat-on-Mouse kit (per instructions) - DAB - 5 min. Washes are with PBS/0.1% Tween Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, November 06, 2014 12:41 PM To: Hans B Snyder; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HELP Hans We use the antibody from Serotec, it works quite nicely. MCA711, proteinase K digestion, rabbit anti-rat secondary and then Envision Rabbit (polymer). We use this antibody at a 1:1200 dilution. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: Thursday, November 06, 2014 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP Hello All, Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14] (ab2557) for mouse tissue? I have been trying to extinguish the background staining but cannot find the right combination. ? We are doing this by hand. ? The recommended dilution for this antibody is 1:50 with HIER. See used protocol below. So far I have tried: 1. concentrations 1:25, 1:50, 1:75, 1:100 - 1:50 gives very high background but good positive staining, 1:100 gives low background but not much positive staining. 2. HIER - 3, 5, 10, 20, minutes @ 96C, 20 minutes at 60C all give the same results - too much back ground 3. We have tried using the H2O2 before the primary and after, increasing the time from 30 minutes to 45 minutes. 4. Tried incubating in the primary for shorter time (20 minutes) and longer overnight at 4C. 5. Tried blocking using 3 different serums, first each one then combinations and longer times. 6. Tried cutting the DAB concentration in 1/2 then 1/3. We have not tried enzyme or acid epitope retrieval yet. *Does anyone have a working protocol or suggestions on what to try?* ?Thank you in advance.? Procedure: Deparaffinize 1. Heat slides to 60C for 10 minutes (oven). 2. Dry slides at room temp 5 minutes. 3. Xylene 5 minutes. 4. Xylene 5 minutes. 5. 100% ethanol 5 minutes (dehydration). 6. 100% ethanol 2 minutes (dehydration). 7. 95% ethanol for 2 minutes (rehydration). 8. 70% ethanol for 2 minutes (rehydration). 9. Run in DI water for 10 minutes. Antigen Retrieval 1. Submerge in 0.01M Citrate Buffer (pH 6.0) at 96C for 5 minutes. 2. Cold running water 5 minutes. Staining 1. Wash in PBS for 1 min. 2. Permeabilize with 0.1% Triton X / 2.5% horse serum + 2.5% fetal bovine serum for 2 hours. 3. Incubate in primary antibody 30-45 minutes at in 2.5% horse serum in PBS. 4. Wash 3x in PBS/tween for 5 minutes each. 5. Fresh 3.0% hydrogen peroxide (H2O2) 2x for 20 minutes each. 6. Wash 3x PBS 2 minutes each. 7. Incubate with anti-Rat Ig impress solution (according to vector?s instructions) 45 minutes. 8. Wash 3x in PBS for 5 minutes each. 9. Prepare Impact DAB substrate (see insert). 10. Using a light microscope, incubate sections with 50-100ul, until desired darkness occurs (5-30 seconds). 11. Stop DAB reaction using deionized water. 12. Wash in DH2O for 5 minutes 13. Counterstain in Harris hematoxylin for 30 seconds. 14. Wash in DH2O for 5 minutes. 15. Dehydrate 2x in 95% alcohol for 30 seconds each. 16. Dehydrate 3x in 100% alcohol for 2 minutes each. 17. Clear 2x in xylene 5 minutes each. 18. Mount coverslips using Leica mounting media. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From bcooper <@t> chla.usc.edu Thu Nov 6 12:58:10 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Nov 6 12:58:16 2014 Subject: [Histonet] Tissue falling off positive slides In-Reply-To: References: Message-ID: We bake ours @ 75-80 degrees for 15 minutes in an offline oven, prior to automated staining. Just a habit I picked up years ago, but I always lean the slide racks diagonally against the side of the oven when they're baking, rather than having them sit entirely upright. They seem to drain any residual water more completely that way. We've used Fisher's positive charged slides for years and haven't really had a problem with tissue loss on H&E. I think your baking temp and time may be a little low and short, respectively. Good luck. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, November 06, 2014 8:36 AM To: Pardue, Judith Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue falling off positive slides Make sure yr sections r airdryed completely before u bake thm Sent from my iPhone > On Nov 6, 2014, at 10:34 AM, "Pardue, Judith" wrote: > > WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. > > Judith Pardue > CHI Memorial > Chatt. Tn. 37343 > Judith_pardue@memorial.org > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Yves.Heremans <@t> vub.ac.be Thu Nov 6 14:12:08 2014 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Thu Nov 6 14:12:13 2014 Subject: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red... Message-ID: <3E3E33D0-F8EF-42EC-8D41-DC4D62E9B101@vub.ac.be> Dear Histonetters, I am trying to combine a peroxidase-DAB staining (brown) with an alkaline phosphatase-staining for detection of two proteins that do not overlap. I have already tried Dako?s Permanent Red and Fuchsin+ and Vector?s Vector Red as AP substrates but I am not getting a clear red color, rather something brownish which is not very different in color from the DAB reaction product. Is there anything I can do to obtain a clearer red color ? Yves From liz <@t> premierlab.com Thu Nov 6 14:43:09 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Nov 6 14:43:15 2014 Subject: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red... In-Reply-To: <3E3E33D0-F8EF-42EC-8D41-DC4D62E9B101@vub.ac.be> References: <3E3E33D0-F8EF-42EC-8D41-DC4D62E9B101@vub.ac.be> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDB91@SBS2K8.premierlab.local> Yves Try Biocares Warp Red, you will still need to air dry the slides prior to coverslipping do not place them in alcohol, air dry, place in xylene and then mount. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Thursday, November 06, 2014 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red... Dear Histonetters, I am trying to combine a peroxidase-DAB staining (brown) with an alkaline phosphatase-staining for detection of two proteins that do not overlap. I have already tried Dako?s Permanent Red and Fuchsin+ and Vector?s Vector Red as AP substrates but I am not getting a clear red color, rather something brownish which is not very different in color from the DAB reaction product. Is there anything I can do to obtain a clearer red color ? Yves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi <@t> nrh-ok.com Thu Nov 6 14:48:35 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Thu Nov 6 14:48:37 2014 Subject: [Histonet] Tissue Processor Validation Procedure Message-ID: <04EE4F75BB5FB246ADB68D69B746044398FF8AD759@MAIL.nrhnt.nrh-ok.com> Hi, I am wondering if you guys in histoland will like to share your Tissue Processor Validation Procedure with me. Thanks, Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From marjoh3 <@t> telus.net Thu Nov 6 15:17:43 2014 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Thu Nov 6 15:17:45 2014 Subject: [Histonet] Reichert Jung Autocut Microtome & LKB Knifemaker For Glycolmethacrylate Blocks For Sale Message-ID: Hi Histonetters, After completing a skin/tumor project by Glycolmethacrylate (GMA), I have a Reichert Jung 1150 Autocut microtome, LKB 2078 Histo Knifemaker and 2 boxes of 1" glass strips for sale. Sections for this project were cut at 2 microns and stained with H&E. Resolution was definitely better in comparison to the routine paraffin sections. I would like to sell all of these items together. Asking $ 4500; best offers will be considered. Marilyn Johnson Edmonton, AB. Canada From pruegghm <@t> hotmail.com Thu Nov 6 17:44:23 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Thu Nov 6 17:44:28 2014 Subject: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red... In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECDB91@SBS2K8.premierlab.local> References: <3E3E33D0-F8EF-42EC-8D41-DC4D62E9B101@vub.ac.be>, <14E2C6176416974295479C64A11CB9AE019C79ECDB91@SBS2K8.premierlab.local> Message-ID: agree Biocares Warp Red is very strong but still has to avoid alcohol. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From: liz@premierlab.com To: Yves.Heremans@vub.ac.be; histonet@lists.utsouthwestern.edu Date: Thu, 6 Nov 2014 13:43:09 -0700 Subject: RE: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red... CC: Yves Try Biocares Warp Red, you will still need to air dry the slides prior to coverslipping do not place them in alcohol, air dry, place in xylene and then mount. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Thursday, November 06, 2014 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Permanent red, Fuchsin and Vector Red not exactly red... Dear Histonetters, I am trying to combine a peroxidase-DAB staining (brown) with an alkaline phosphatase-staining for detection of two proteins that do not overlap. I have already tried Dako?s Permanent Red and Fuchsin+ and Vector?s Vector Red as AP substrates but I am not getting a clear red color, rather something brownish which is not very different in color from the DAB reaction product. Is there anything I can do to obtain a clearer red color ? Yves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Millard <@t> prlnet.com Fri Nov 7 09:55:02 2014 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Fri Nov 7 09:55:20 2014 Subject: [Histonet] Vantage in Grossing Room Message-ID: <64D11CD058311340A1966C234C6B0B893D6EF6DD@PRLEXCH02.prlnet.com> We currently have Vantage, and are using Vantage for grossing, but are going to move grossing out of Vantage and into CoPath, starting in Vantage at embedding. After re-evaluating grossing workflow, we decided the work required adding and canceling blocks and stains at grossing, going between 2 systems (have to add in CoPath, then manually push to Vantage, and wait for the blocks to cross the interface, then take the container to cassette printer and scan to get new cassettes) was adding too much time and workflow interruption to grossing. The disadvantage is then there isn't total case visibility in Vantage. We observed a bidirectional interface with Dako using CoPath and grossing in CoPath, and really liked it. The entire case is then visible in both systems, and full reports can come out of CoPath. CoPath has told us that the bidirectional does not work as well with Vantage as with other vendors, but I don't know how accurate or to what degree that is--I haven't seen bidirectional in action with Vantage to compare. Whenever we talked "status updates" in the past, we considered only our current CoPath statuses (ordered, printed, verified), but with AB&T those statuses can be defined and can include "grossed" "embedded" "sectioned," a pathologist's initials, filed...though how those statuses work with Vantage bidirectional, I'm not sure. Questions to ask if you consider bidirectional. You could consider using CoPath AB&T throughout--you would have that capability with the AB&T that you need to run a third party solution, but CoPath labels will not drive Ventana stainers, and that would require CoPath seat licenses for every workstation. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, November 06, 2014 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Vantage in Grossing Room (Morken, Timothy) 2. RE: Histonet Digest, Vol 132, Issue 6 (Cary Ward) 3. RE: Vantage in Grossing Room (Michael Mihalik) 4. Leica ASP6025 Processor (Paula Lucas) 5. Placentas (Fortin, Joyce) 6. Placenta's (mtitford@aol.com) 7. RE: Leica ASP6025 Processor (Jamal) 8. RE: Cold plates for icing blocks? (Sanders, Jeanine (CDC/OID/NCEZID)) 9. RE: Cold plates for icing blocks? (Boyd, Debbie M) 10. HISTO/CYTO SKILL REVIEW (rmweber113@comcast.net) 11. 2 Sucinyl Cystine stain (AKA 2SC) (Sebree Linda A) 12. Tissue falling off positive slides (Pardue, Judith) 13. Re: Tissue falling off positive slides (Patsy Ruegg) 14. HELP (Hans B Snyder) 15. Re: Tissue falling off positive slides (Tony Auge) 16. RE: HISTO/CYTO SKILL REVIEW (Tom McNemar) 17. RE: HELP (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 Nov 2014 18:31:41 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] Vantage in Grossing Room To: 'Michael Mihalik' , 'Histonet' Message-ID: <761E2B5697F795489C8710BCC72141FF367B4E60@ex07.net.ucsf.edu> Content-Type: text/plain; charset="iso-8859-1" AB&T is the Copath tracking system but when a third party tracking system is used AB&T is used as the interface to the third party system. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 9:09 AM To: Morken, Timothy; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room So AB&T is the actual interface, not their barcode and tracking system? I understand about the ordering of blocks and stains, etc. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 05, 2014 11:06 AM To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 5 Nov 2014 13:35:51 -0500 From: "Cary Ward" Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 6 To: Message-ID: <00b701cff927$53d93ba0$fb8bb2e0$@com> When he let's us know, is he calling you or me? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Animal Tissue/Human Tissue (Anne Murvosh) 2. IHC Validation (Arrington, Karla A) 3. cardiac leaflet processing (Helen Ilsley) 4. RE: IHC Validation (Sebree Linda A) 5. RE: Vantage in Grossing Room (Michael Mihalik) 6. RE: Vantage in Grossing Room (Morken, Timothy) 7. B-5 fixative (Moe, Barbi A) 8. RE: Vantage in Grossing Room (Michael Mihalik) ---------------------------------------------------------------------- Message: 1 Date: Tue, 4 Nov 2014 18:12:45 +0000 From: Anne Murvosh Subject: [Histonet] RE: Animal Tissue/Human Tissue To: Matthew Lauterbach , "'histonet@lists.utsouthwestern.edu'" Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B701043B@EX-01.Advancederm.net> Content-Type: text/plain; charset="us-ascii" My first job was at a lab that did both human and animal (till a case got mixed up) the only difference I found was animals were fattier and hairier. Same microtome. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Lauterbach Sent: Tuesday, November 04, 2014 8:39 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Animal Tissue/Human Tissue Has anyone used the same microtome to cut animal tissue as well as human tissue? We are a hospital clinical Histology lab being asked to provide research study slides from nonhuman sources. Anyone have an input or precedence? I couldn't find anything that specifically prohibits this practice from the CAP. Thanks for the help in advance!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 5 Nov 2014 04:26:51 +0000 From: "Arrington, Karla A" Subject: [Histonet] IHC Validation To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I am validating IHC's. What is the CAP requirements regarding negative slides. I've asked CAP but have not received a response. Are 10 negative patient tissues ran for each antibody using the negative protocol or are they ran using each antibody but also a known Negative control ran using the negative protocol? Thanks! Karla Arrington, HT (ASCP) HIT (AHIMA) Supervisor of Pathology ANMC Pathology kaarrington@anthc.org ------------------------------ Message: 3 Date: Wed, 5 Nov 2014 06:35:54 +0000 From: Helen Ilsley Subject: [Histonet] cardiac leaflet processing To: "histonet@lists.utsouthwestern.edu" Message-ID: <2CBD60D7-4BA6-4DB4-B34C-6872878FFBD3@uct.ac.za> Content-Type: text/plain; charset=WINDOWS-1252 HI Does anyone have any experience with the processing of cardiac leaflets, I am finding they tend to curl and make orientation and embedding difficult. I am now laying them flat between some foam and hoping that this will work as I am sure it is going into the wax that makes the leaflet curl They look fine as they come out of the last xylene before entering the wax. Can one get wax that has a lower melting point than 55C Any advice would be appreciated. Tx Helen Ilsley Chief Medical Technologist Cardiovascular Research Unit UCT Anzio Road Observatory Cape Town ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ------------------------------ Message: 4 Date: Wed, 5 Nov 2014 13:36:54 +0000 From: Sebree Linda A Subject: [Histonet] RE: IHC Validation To: "'Arrington, Karla A'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <77DD817201982748BC67D7960F2F76AF0E560E@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Karla, We try to run 1/2 of the required cases with known negative cases and the other 1/2 with known positive cases. In addition, all cases are run with a negative reagent control in place of the antibody using the optimized protocol for the antibody. These are your negative reagent controls. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A Sent: Tuesday, November 04, 2014 10:27 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] IHC Validation I am validating IHC's. What is the CAP requirements regarding negative slides. I've asked CAP but have not received a response. Are 10 negative patient tissues ran for each antibody using the negative protocol or are they ran using each antibody but also a known Negative control ran using the negative protocol? Thanks! Karla Arrington, HT (ASCP) HIT (AHIMA) Supervisor of Pathology ANMC Pathology kaarrington@anthc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 5 Nov 2014 10:12:26 -0500 From: "Michael Mihalik" Subject: RE: [Histonet] Vantage in Grossing Room To: "'Amber McKenzie'" , "'Tom McNemar'" , "'Morken, Timothy'" , "'Histonet'" Message-ID: <007601cff90a$e9b49970$bd1dcc50$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope Im making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 5 Nov 2014 16:05:30 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] Vantage in Grossing Room To: 'Michael Mihalik' , 'Amber McKenzie' , 'Tom McNemar' , 'Histonet' Message-ID: <761E2B5697F795489C8710BCC72141FF367B4DE0@ex07.net.ucsf.edu> Content-Type: text/plain; charset="iso-8859-1" Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 5 Nov 2014 16:53:11 +0000 From: "Moe, Barbi A" Subject: [Histonet] B-5 fixative To: "histonet@lists.utsouthwestern.edu" Message-ID: <8B4B31D17067E540AC760B4A30190B99014A4055DB@LXEXMB03.gundluth.org> Content-Type: text/plain; charset="iso-8859-1" Can anyone tell me why B-5 fixative is called "B-5"? Does the "B" stand for something, or does the "5" have a meaning? Any thoughts are greatly appreciated! Barb Moe Histology Lab Gundersen Health System La Crosse WI bamoe@gundersenhealth.org ------------------------------ Message: 8 Date: Wed, 5 Nov 2014 12:08:43 -0500 From: "Michael Mihalik" Subject: RE: [Histonet] Vantage in Grossing Room To: "'Morken, Timothy'" , "'Amber McKenzie'" , "'Tom McNemar'" , "'Histonet'" Message-ID: <004001cff91b$27f9a8a0$77ecf9e0$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" So AB&T is the actual interface, not their barcode and tracking system? I understand about the ordering of blocks and stains, etc. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 05, 2014 11:06 AM To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 6 **************************************** ------------------------------ Message: 3 Date: Wed, 5 Nov 2014 13:40:08 -0500 From: "Michael Mihalik" Subject: RE: [Histonet] Vantage in Grossing Room To: "'Morken, Timothy'" , "'Histonet'" Message-ID: <007101cff927$ed5f4bc0$c81de340$@pathview.com> Content-Type: text/plain; charset="iso-8859-1" I think I got it. Thank you for your explanation and most importantly, your time. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 05, 2014 1:32 PM To: 'Michael Mihalik'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room AB&T is the Copath tracking system but when a third party tracking system is used AB&T is used as the interface to the third party system. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 9:09 AM To: Morken, Timothy; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room So AB&T is the actual interface, not their barcode and tracking system? I understand about the ordering of blocks and stains, etc. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Wednesday, November 05, 2014 11:06 AM To: 'Michael Mihalik'; 'Amber McKenzie'; 'Tom McNemar'; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Cerner Copath AB&T will not interact in a bi-directional way with a third party tracking system (except to get "status" updates, ie when a block is scanned at embedding Vantage will send a message to Copath to update the block status). That is, you cannot enter a block or stain order in the third party system and have the data be written back to the Copath database. You must enter all in Copath and then the third party system picks it up from there. That is why Copath was telling Anita that they would need to use Copath alone at grossing (and accessioning, and all stain ordering). Also, You must buy and install AB&T to use any third party tracking system (AB&T is the HL7 interface). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, November 05, 2014 7:12 AM To: 'Amber McKenzie'; 'Tom McNemar'; Morken, Timothy; 'Histonet' Subject: RE: [Histonet] Vantage in Grossing Room Amber, at the risk of exposing my ignorance, doesnt AB&T and Vantage offer very similar functionality? I'm just curious why you would have both? As to your question, if you have some validation process occurring at grossing that requires information from your Copath system, then perhaps you might want to use Copath at grossing. For instance, if you scan requisitions or manual drawings or anything of that nature and you require those scanned images or other information to be verified at grossing, you might want to use Copath for grossing IF that information is not being sent across the interface from Copath to Vantage. I hope I'm making sense, but if not, please feel free to ask for clarification. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, November 04, 2014 9:49 AM To: Tom McNemar; 'Morken, Timothy'; Histonet Subject: [Histonet] Vantage in Grossing Room For those of you who have Vantage, in your grossing room do you have Vantage set up or your LIS system? I was thinking it should be vantage, but our Co-path person said we should have AB&T set up in the grossing room and let vantage start at embedding, microtomy and case assembly. What are your thoughts? Do you have a full computer, keyboard, and scanner in grossing? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 5 Nov 2014 13:09:56 -0800 From: "Paula Lucas" Subject: [Histonet] Leica ASP6025 Processor To: Message-ID: <2EDC1A8E8F12465F8FD0BCCFA0E47F08@biopath.local> Content-Type: text/plain; charset="us-ascii" Hello, We're considering the Leica ASP6025 processor for our laboratory and I was hoping I can get pros and cons or any comments such as in performance, reagent money savings, ease of use, any comment from you. Thank you so much in advance for taking the time to give me your thoughts, Paula Lucas Lab Manager Bio-Path Medical Group California ------------------------------ Message: 5 Date: Wed, 5 Nov 2014 16:51:16 -0500 From: "Fortin, Joyce" Subject: [Histonet] Placentas To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Would anyone be willing to share their policy for handling placentas? I have Public Health asking! Thanks! UHS of Delaware, Inc. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited, and may be punishable by law. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 6 Date: Wed, 5 Nov 2014 21:05:29 -0500 From: mtitford@aol.com Subject: [Histonet] Placenta's To: histonet@lists.utsouthwestern.edu Message-ID: <8D1C77D45AE217B-D34-51270@webmail-va081.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Joyce Fortin asks about placenta's: I work in a teaching hospital with a busy L&D department and we examine every placenta from every birth. Depending on their history the placenta may be "gross in" (with four blocks) or "gross only". The physicians are happy to have a record of the weight and appearance and dimensions, and microscopic appearance Other area hospitals, however do not examine their placenta. Michael Titford Pathology USA Mobile AL ------------------------------ Message: 7 Date: Thu, 6 Nov 2014 09:57:22 +0300 From: "Jamal" Subject: RE: [Histonet] Leica ASP6025 Processor To: "'Paula Lucas'" , Message-ID: <002c01cff98e$eb628b90$c227a2b0$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="windows-1256" Good day Paula Lucas I have Leica ASP6025 processor since about 6 months. I am processing about 200 to 250 biopsy cassettes on that machine and I recommend to have it, it's smart machine, very easy to use and reagent money savings. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Thursday, November 06, 2014 12:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica ASP6025 Processor Hello, We're considering the Leica ASP6025 processor for our laboratory and I was hoping I can get pros and cons or any comments such as in performance, reagent money savings, ease of use, any comment from you. Thank you so much in advance for taking the time to give me your thoughts, Paula Lucas Lab Manager Bio-Path Medical Group California _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 6 Nov 2014 13:53:22 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] RE: Cold plates for icing blocks? To: "Morken, Timothy" , Histonet Message-ID: <3B2CD438E1628A41BD687E98B963B7811FE445E3@EMBX-CHAM2.cdc.gov> Content-Type: text/plain; charset="us-ascii" We do when we are sectioning blocks that were processed on the TissueTek Xpress as too much water causes poor staining with these. Jeanine Sanders CDc Atlanta ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Monday, November 03, 2014 2:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 6 Nov 2014 14:11:19 +0000 From: "Boyd, Debbie M" Subject: [Histonet] RE: Cold plates for icing blocks? To: "Sanders, Jeanine (CDC/OID/NCEZID)" , "Morken, Timothy" , Histonet Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9EF3A08@TN001WEXMBX014.US.chs.net> Content-Type: text/plain; charset="us-ascii" We use frozen water in Rubbermaid containers. We use the medium size that will last through the cutting of at least 50-60 blocks. As we move blocks off the ice we add uncut blocks. Yes, the ice melts as the morning progresses, but we but gauze on top of the ice to keep the blocks for setting in water. The ice is about an 1 in to 1.5 inches deep so it doesn't melt that fast. Also our room temp is about 67-68 degrees. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sanders, Jeanine (CDC/OID/NCEZID) [jqb7@cdc.gov] Sent: Thursday, November 06, 2014 8:53 AM To: Morken, Timothy; Histonet Subject: [Histonet] RE: Cold plates for icing blocks? We do when we are sectioning blocks that were processed on the TissueTek Xpress as too much water causes poor staining with these. Jeanine Sanders CDc Atlanta ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Monday, November 03, 2014 2:29 PM To: Histonet Subject: [Histonet] Cold plates for icing blocks? Does anyone use a cold plate, like that used for embedding, for icing blocks for sectioning? Just an idea.... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 10 Date: Thu, 6 Nov 2014 14:56:28 +0000 (UTC) From: rmweber113@comcast.net Subject: [Histonet] HISTO/CYTO SKILL REVIEW To: histonet Message-ID: <616228225.3786732.1415285788476.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 Hi,?? I was wondering if anyone knows how often Joint Commission requires cytologist and histologist to have their competency reviewed.?? I'm not talking about their job performance review, but how often to review their job skills with a check list under direct observation.?? We have been doing it quarterly for histologists. Thanks so much, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC ------------------------------ Message: 11 Date: Thu, 6 Nov 2014 16:00:16 +0000 From: Sebree Linda A Subject: [Histonet] 2 Sucinyl Cystine stain (AKA 2SC) To: "'Histonet (Histonet@lists.utsouthwestern.edu)'" Message-ID: <77DD817201982748BC67D7960F2F76AF0E58B4@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Good morning, Does anyone know of a reference lab offering this IHC stain for human FFPE tissue? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ------------------------------ Message: 12 Date: Thu, 6 Nov 2014 15:38:25 +0000 From: "Pardue, Judith" Subject: [Histonet] Tissue falling off positive slides To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 Judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 13 Date: Thu, 6 Nov 2014 10:36:25 -0600 From: Patsy Ruegg Subject: Re: [Histonet] Tissue falling off positive slides To: "Pardue, Judith" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Make sure yr sections r airdryed completely before u bake thm Sent from my iPhone > On Nov 6, 2014, at 10:34 AM, "Pardue, Judith" wrote: > > WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. > > Judith Pardue > CHI Memorial > Chatt. Tn. 37343 > Judith_pardue@memorial.org > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 6 Nov 2014 12:30:41 -0500 From: Hans B Snyder Subject: [Histonet] HELP To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Hello All, Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14] (ab2557) for mouse tissue? I have been trying to extinguish the background staining but cannot find the right combination. ??? We are doing this by hand. ??? The recommended dilution for this antibody is 1:50 with HIER. See used protocol below. So far I have tried: 1. concentrations 1:25, 1:50, 1:75, 1:100 - 1:50 gives very high background but good positive staining, 1:100 gives low background but not much positive staining. 2. HIER - 3, 5, 10, 20, minutes @ 96C, 20 minutes at 60C all give the same results - too much back ground 3. We have tried using the H2O2 before the primary and after, increasing the time from 30 minutes to 45 minutes. 4. Tried incubating in the primary for shorter time (20 minutes) and longer overnight at 4C. 5. Tried blocking using 3 different serums, first each one then combinations and longer times. 6. Tried cutting the DAB concentration in 1/2 then 1/3. We have not tried enzyme or acid epitope retrieval yet. *Does anyone have a working protocol or suggestions on what to try?* ???Thank you in advance.??? Procedure: Deparaffinize 1. Heat slides to 60C for 10 minutes (oven). 2. Dry slides at room temp 5 minutes. 3. Xylene 5 minutes. 4. Xylene 5 minutes. 5. 100% ethanol 5 minutes (dehydration). 6. 100% ethanol 2 minutes (dehydration). 7. 95% ethanol for 2 minutes (rehydration). 8. 70% ethanol for 2 minutes (rehydration). 9. Run in DI water for 10 minutes. Antigen Retrieval 1. Submerge in 0.01M Citrate Buffer (pH 6.0) at 96C for 5 minutes. 2. Cold running water 5 minutes. Staining 1. Wash in PBS for 1 min. 2. Permeabilize with 0.1% Triton X / 2.5% horse serum + 2.5% fetal bovine serum for 2 hours. 3. Incubate in primary antibody 30-45 minutes at in 2.5% horse serum in PBS. 4. Wash 3x in PBS/tween for 5 minutes each. 5. Fresh 3.0% hydrogen peroxide (H2O2) 2x for 20 minutes each. 6. Wash 3x PBS 2 minutes each. 7. Incubate with anti-Rat Ig impress solution (according to vector???s instructions) 45 minutes. 8. Wash 3x in PBS for 5 minutes each. 9. Prepare Impact DAB substrate (see insert). 10. Using a light microscope, incubate sections with 50-100ul, until desired darkness occurs (5-30 seconds). 11. Stop DAB reaction using deionized water. 12. Wash in DH2O for 5 minutes 13. Counterstain in Harris hematoxylin for 30 seconds. 14. Wash in DH2O for 5 minutes. 15. Dehydrate 2x in 95% alcohol for 30 seconds each. 16. Dehydrate 3x in 100% alcohol for 2 minutes each. 17. Clear 2x in xylene 5 minutes each. 18. Mount coverslips using Leica mounting media. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com ------------------------------ Message: 15 Date: Thu, 6 Nov 2014 10:31:47 -0700 From: Tony Auge Subject: Re: [Histonet] Tissue falling off positive slides To: Patsy Ruegg Cc: "histonet@lists.utsouthwestern.edu" , "Pardue, Judith" Message-ID: Content-Type: text/plain; charset=UTF-8 Increase the time and/or temperature. I bake mine at 85 Celsius for 20 minutes on non charged slides. Section thickness is also a factor as well as the pH of your blueing reagent. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com ------------------------------ Message: 16 Date: Thu, 6 Nov 2014 12:40:58 -0500 From: Tom McNemar Subject: [Histonet] RE: HISTO/CYTO SKILL REVIEW To: "'rmweber113@comcast.net'" , histonet Message-ID: Content-Type: text/plain; charset="utf-8" We are JCAHO inspected (just this year) and only do yearly competencies. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rmweber113@comcast.net Sent: Thursday, November 06, 2014 9:56 AM To: histonet Subject: [Histonet] HISTO/CYTO SKILL REVIEW Hi, I was wondering if anyone knows how often Joint Commission requires cytologist and histologist to have their competency reviewed. I'm not talking about their job performance review, but how often to review their job skills with a check list under direct observation. We have been doing it quarterly for histologists. Thanks so much, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 17 Date: Thu, 6 Nov 2014 10:41:12 -0700 From: Elizabeth Chlipala Subject: RE: [Histonet] HELP To: Hans B Snyder , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDB7E@SBS2K8.premierlab.local> Content-Type: text/plain; charset="utf-8" Hans We use the antibody from Serotec, it works quite nicely. MCA711, proteinase K digestion, rabbit anti-rat secondary and then Envision Rabbit (polymer). We use this antibody at a 1:1200 dilution. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: Thursday, November 06, 2014 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP Hello All, Does anyone have a protocol for the Anti-Neutrophil antibody [NIMP-R14] (ab2557) for mouse tissue? I have been trying to extinguish the background staining but cannot find the right combination. ??? We are doing this by hand. ??? The recommended dilution for this antibody is 1:50 with HIER. See used protocol below. So far I have tried: 1. concentrations 1:25, 1:50, 1:75, 1:100 - 1:50 gives very high background but good positive staining, 1:100 gives low background but not much positive staining. 2. HIER - 3, 5, 10, 20, minutes @ 96C, 20 minutes at 60C all give the same results - too much back ground 3. We have tried using the H2O2 before the primary and after, increasing the time from 30 minutes to 45 minutes. 4. Tried incubating in the primary for shorter time (20 minutes) and longer overnight at 4C. 5. Tried blocking using 3 different serums, first each one then combinations and longer times. 6. Tried cutting the DAB concentration in 1/2 then 1/3. We have not tried enzyme or acid epitope retrieval yet. *Does anyone have a working protocol or suggestions on what to try?* ???Thank you in advance.??? Procedure: Deparaffinize 1. Heat slides to 60C for 10 minutes (oven). 2. Dry slides at room temp 5 minutes. 3. Xylene 5 minutes. 4. Xylene 5 minutes. 5. 100% ethanol 5 minutes (dehydration). 6. 100% ethanol 2 minutes (dehydration). 7. 95% ethanol for 2 minutes (rehydration). 8. 70% ethanol for 2 minutes (rehydration). 9. Run in DI water for 10 minutes. Antigen Retrieval 1. Submerge in 0.01M Citrate Buffer (pH 6.0) at 96C for 5 minutes. 2. Cold running water 5 minutes. Staining 1. Wash in PBS for 1 min. 2. Permeabilize with 0.1% Triton X / 2.5% horse serum + 2.5% fetal bovine serum for 2 hours. 3. Incubate in primary antibody 30-45 minutes at in 2.5% horse serum in PBS. 4. Wash 3x in PBS/tween for 5 minutes each. 5. Fresh 3.0% hydrogen peroxide (H2O2) 2x for 20 minutes each. 6. Wash 3x PBS 2 minutes each. 7. Incubate with anti-Rat Ig impress solution (according to vector???s instructions) 45 minutes. 8. Wash 3x in PBS for 5 minutes each. 9. Prepare Impact DAB substrate (see insert). 10. Using a light microscope, incubate sections with 50-100ul, until desired darkness occurs (5-30 seconds). 11. Stop DAB reaction using deionized water. 12. Wash in DH2O for 5 minutes 13. Counterstain in Harris hematoxylin for 30 seconds. 14. Wash in DH2O for 5 minutes. 15. Dehydrate 2x in 95% alcohol for 30 seconds each. 16. Dehydrate 3x in 100% alcohol for 2 minutes each. 17. Clear 2x in xylene 5 minutes each. 18. Mount coverslips using Leica mounting media. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 7 **************************************** CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From Kristofer.Rodriguez <@t> bcm.edu Fri Nov 7 11:06:14 2014 From: Kristofer.Rodriguez <@t> bcm.edu (Rodriguez, Kristofer) Date: Fri Nov 7 11:10:06 2014 Subject: [Histonet] Macrophage Message-ID: <34b95be5ad844dfda6da65c28e787e9f@BN3PR0601MB1138.namprd06.prod.outlook.com> I am going to perform a CCRL2 IHC Stain on some mouse lung, my control has already been made and put on a slide, it is a macrophage. Does anybody know how I should prep the macrophage slide for staining? Do I treat it like a cytospin and start it in 95% ETOH then to DI H2O to TBS? From Glenn.Hauck <@t> albertahealthservices.ca Fri Nov 7 11:38:02 2014 From: Glenn.Hauck <@t> albertahealthservices.ca (Glenn Hauck) Date: Fri Nov 7 11:38:12 2014 Subject: [Histonet] AFB stained smears Message-ID: <38FA8FCA474F834CB9B90590D7C72390016E13820454@EXMBX4C.crha.bewell.ca> The odd time I receive a request to do a Zeihl Neelsen stain for acid fast bacteria on a smear for Micro. My question is what kind of control should I be using? I have tissue processed with AFB, but I feel this should not be used because the smear is not processed the same way as my AFB control block. Thanks Glenn Hauck Charge Technologist Pathology Queen Elizabeth II Hospital Grande Prairie, AB T8V 2E8 780-538-7429 Work Main 780-538-7184 Work Office glenn.hauck@albertahealthservices.ca ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From Richard.Cartun <@t> hhchealth.org Fri Nov 7 13:05:37 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Nov 7 13:05:44 2014 Subject: [Histonet] CMV IHC Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355EA383@HHCEXCHMB03.hhcsystem.org> Is anyone using the "E13" clone for CMV detection by immunohistochemistry? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From pruegghm <@t> hotmail.com Fri Nov 7 13:48:29 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Fri Nov 7 13:48:38 2014 Subject: [Histonet] AFB stained smears In-Reply-To: <38FA8FCA474F834CB9B90590D7C72390016E13820454@EXMBX4C.crha.bewell.ca> References: <38FA8FCA474F834CB9B90590D7C72390016E13820454@EXMBX4C.crha.bewell.ca> Message-ID: U r right ffpe control would not be ideal but unless u have access to a source + for tb u could make a bunch of smears to keep for controls that may be your only option Sent from my iPhone > On Nov 7, 2014, at 10:38 AM, "Glenn Hauck" wrote: > > The odd time I receive a request to do a Zeihl Neelsen stain for acid fast bacteria on a smear for Micro. My question is what kind of control should I be using? I have tissue processed with AFB, but I feel this should not be used because the smear is not processed the same way as my AFB control block. > > Thanks > > Glenn Hauck > Charge Technologist > Pathology > Queen Elizabeth II Hospital > Grande Prairie, AB T8V 2E8 > > 780-538-7429 Work Main > 780-538-7184 Work Office > glenn.hauck@albertahealthservices.ca > > > ________________________________ > This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From VEBain <@t> mdanderson.org Fri Nov 7 16:01:24 2014 From: VEBain <@t> mdanderson.org (Bain,Virginia) Date: Fri Nov 7 16:01:31 2014 Subject: [Histonet] anti-roll plate damage? Message-ID: Hello again, First I wanted to thank everyone for their helpful replies. To answer an earlier question, yes we do use the Sakura low profile blades but we are using them without issue on our microtome (also a low profile blade holder). I especially wanted to thank the folks at Thermo Scientific who upon finding out that we were having trouble have been incredibly helpful. They have been very nice about letting me know that I should never feel that I cannot contact them. Finally, in case anyone encounters an issue like this in the future, their diagnosis was that some user may have been cutting with the anti-roll plate too far forward. Thanks again to everyone for their assistance. Sincerely, Virginia -- Virginia Bain Postdoctoral Fellow Richie Lab M D Anderson - Science Park 512-237-6443 On 11/3/14, 11:34 AM, "Bain,Virginia" wrote: >Greetings, > > Our lab recently got a new cryostat (a cryostar NX50). It sees light to >moderate usage (3 - 20 hours a week) and has been operational for about 3 >weeks. It has had a host of problems since we received it (shipped with a >busted motor and the sensor in the window did not detect when it was shut >which lead to some kind of short and killed the lighting system). I am >hesitant to contact the rep again (they?ve told us they?re tired of >hearing from us) until I?m certain this is not user error of some sort. > > For the most part we have been getting nice clean sections. Every so >often we start getting lines and tears which continue even when the blade >is moved or replaced. Twice now we have resolved this issue by rotating >the anti-roll plate to a smooth edge. When we encounter this problem I >find that the anti-roll plate is jagged to the touch, which is I think >what is causing the tears. I see that some people suggest smoothing the >anti-roll plate out on an emory board when it gets rough but this seems >very early in the life of the anti-roll plate to me. In my previous lab >we used the same anti-roll plate without issue for about 2 years with >heavy usage (50-70 hours a week) and so I?m wondering why this anti-roll >plate is encountering so much damage? > >Thanks for the help! > >-- >Virginia Bain >Postdoctoral Fellow >Richie Lab >M D Anderson - Science Park >512-237-6443 > > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Sat Nov 8 02:57:33 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Sat Nov 8 02:58:06 2014 Subject: [Histonet] Tissue Processor Validation Procedure In-Reply-To: <04EE4F75BB5FB246ADB68D69B746044398FF8AD759@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B746044398FF8AD759@MAIL.nrhnt.nrh-ok.com> Message-ID: <00b701cffb32$0aa955c0$1ffc0140$@rowaihi@alborglaboratories.com> Dear colleague I hope the attached help you Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Thursday, November 06, 2014 11:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation Procedure Hi, I am wondering if you guys in histoland will like to share your Tissue Processor Validation Procedure with me. Thanks, Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Sat Nov 8 04:31:27 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Sat Nov 8 04:31:14 2014 Subject: [Histonet] Tissue falling off positive slides In-Reply-To: References: Message-ID: <00c001cffb3f$297ea1f0$7c7be5d0$@rowaihi@alborglaboratories.com> Do you use Autostainer? What brand ? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Thursday, November 06, 2014 6:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue falling off positive slides WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 Judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Sat Nov 8 07:42:23 2014 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Sat Nov 8 07:42:28 2014 Subject: [Histonet] anti-roll plate damage? In-Reply-To: References: Message-ID: Hey everyone, don't forget to "reply all" when answering questions! Is there a way to change the reply function for this list so you can reply to the list instead just the person asking a question? I don't want to miss out on any answers and I think for this question I didn't see any but the thank you. Pitt just changed their email system and their virus filter is a little too stringent, so my not getting replies could be due to that. But I would love to have a standard "reply to list" option. Can we get that set up? Feel free to naysay me if the mods have reasons for not doing so. Emily On Nov 3, 2014 12:34 PM, "Bain,Virginia" wrote: > Greetings, > > Our lab recently got a new cryostat (a cryostar NX50). It sees > light to > moderate usage (3 - 20 hours a week) and has been operational for about 3 > weeks. It has had a host of problems since we received it (shipped with a > busted motor and the sensor in the window did not detect when it was shut > which lead to some kind of short and killed the lighting system). I am > hesitant to contact the rep again (they?ve told us they?re tired of > hearing from us) until I?m certain this is not user error of some sort. > > For the most part we have been getting nice clean sections. Every > so > often we start getting lines and tears which continue even when the blade > is moved or replaced. Twice now we have resolved this issue by rotating > the anti-roll plate to a smooth edge. When we encounter this problem I > find that the anti-roll plate is jagged to the touch, which is I think > what is causing the tears. I see that some people suggest smoothing the > anti-roll plate out on an emory board when it gets rough but this seems > very early in the life of the anti-roll plate to me. In my previous lab > we used the same anti-roll plate without issue for about 2 years with > heavy usage (50-70 hours a week) and so I?m wondering why this anti-roll > plate is encountering so much damage? > > Thanks for the help! > > -- > Virginia Bain > Postdoctoral Fellow > Richie Lab > M D Anderson - Science Park > 512-237-6443 > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rbasilio9 <@t> sbcglobal.net Sat Nov 8 08:23:14 2014 From: rbasilio9 <@t> sbcglobal.net (rbasilio9) Date: Sat Nov 8 08:24:21 2014 Subject: [Histonet] anti-roll plate damage? Message-ID: Thanks Emily for bringing this up. ?The spirit of this great website and all its members is to share information and experiences... ?We all get the benefits.? Thank you all.? Sent from my Verizon Wireless 4G LTE smartphone
-------- Original message --------
From: Emily Brown
Date:11/08/2014 05:42 (GMT-08:00)
To:
Cc: Histonet
Subject: Re: [Histonet] anti-roll plate damage?
Hey everyone, don't forget to "reply all" when answering questions! Is there a way to change the reply function for this list so you can reply to the list instead just the person asking a question? I don't want to miss out on any answers and I think for this question I didn't see any but the thank you. Pitt just changed their email system and their virus filter is a little too stringent, so my not getting replies could be due to that. But I would love to have a standard "reply to list" option. Can we get that set up? Feel free to naysay me if the mods have reasons for not doing so. Emily On Nov 3, 2014 12:34 PM, "Bain,Virginia" wrote: > Greetings, > > Our lab recently got a new cryostat (a cryostar NX50). It sees > light to > moderate usage (3 - 20 hours a week) and has been operational for about 3 > weeks. It has had a host of problems since we received it (shipped with a > busted motor and the sensor in the window did not detect when it was shut > which lead to some kind of short and killed the lighting system). I am > hesitant to contact the rep again (they?ve told us they?re tired of > hearing from us) until I?m certain this is not user error of some sort. > > For the most part we have been getting nice clean sections. Every > so > often we start getting lines and tears which continue even when the blade > is moved or replaced. Twice now we have resolved this issue by rotating > the anti-roll plate to a smooth edge. When we encounter this problem I > find that the anti-roll plate is jagged to the touch, which is I think > what is causing the tears. I see that some people suggest smoothing the > anti-roll plate out on an emory board when it gets rough but this seems > very early in the life of the anti-roll plate to me. In my previous lab > we used the same anti-roll plate without issue for about 2 years with > heavy usage (50-70 hours a week) and so I?m wondering why this anti-roll > plate is encountering so much damage? > > Thanks for the help! > > -- > Virginia Bain > Postdoctoral Fellow > Richie Lab > M D Anderson - Science Park > 512-237-6443 > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Nov 10 11:05:06 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon Nov 10 11:05:26 2014 Subject: [Histonet] tendon help Message-ID: <3B2CD438E1628A41BD687E98B963B7811FE5238A@EMBX-CHAM2.cdc.gov> Morning everyone, Can you please send me your best techniques, including processing schedules, for human tendon? Thanks, Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov From kstoll <@t> mcw.edu Mon Nov 10 11:11:03 2014 From: kstoll <@t> mcw.edu (Stoll, Kathryn) Date: Mon Nov 10 11:11:09 2014 Subject: [Histonet] Microm 335E Retraction Message-ID: I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Milwaukee WI 53226 Phone: 414-805-1525 Fax: 414-805-1528 From Joyce.Weems <@t> emoryhealthcare.org Mon Nov 10 15:39:22 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Nov 10 15:39:32 2014 Subject: [Histonet] CPT Code changes Message-ID: Have you all seen this news? "To eliminate these discrepancies, CPT(r) 2015 revises parent code 88342, imunohistochemistry or immunocytochemistry, per specimen; initial single antibody stain procedure, deletes indented code 88343, and adds two new indented codes, +88341, each additional single antibody stain procedure, and 88344, each multiplex antibody stain procedure." Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From udsd007 <@t> gmail.com Mon Nov 10 21:16:32 2014 From: udsd007 <@t> gmail.com (Mike Andrews) Date: Mon Nov 10 21:16:45 2014 Subject: [Histonet] Microm 335E Retraction In-Reply-To: References: Message-ID: <228C4E7F-1815-42E0-BFE5-519A92BFE008@gmail.com> A complete user manual is available at http://www.bu.edu/becf/downloads/BioInterface%20Technologies/HM%20355S%20Manual.pdf Mike Andrews, W5EGO WWME Oklahoma area executive team > On Nov 10, 2014, at 11:11 AM, Stoll, Kathryn wrote: > > I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? > > Kathryn Stoll > Supervisor Histology > Clinical and Translational Research Core Lab > Medical College of Wisconsin > 9200 W. Wisconsin Ave Room 1176 > Milwaukee WI 53226 > Phone: 414-805-1525 > Fax: 414-805-1528 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From richard.vernon <@t> thermofisher.com Tue Nov 11 01:37:57 2014 From: richard.vernon <@t> thermofisher.com (Vernon, Richard I.) Date: Tue Nov 11 01:38:06 2014 Subject: [Histonet] RE: Microm 335E Retraction In-Reply-To: <20141110180217.86967E4C527@usmx01.thermofisher.com> References: <20141110180217.86967E4C527@usmx01.thermofisher.com> Message-ID: <8411B0BF0CE50F4EA179AB3A06DB5C03560F35EB50@UKHIG-MXVS01.emea.thermo.com> Dear Kathryn, The retraction on the HM355E can be turned off by simultaneously pressing the "A" button and the button to the left of this (with the double arrow on it). The retraction light (yellow LED) will then go off. If the light still remains on turn the hand wheel once and it should go off confirming your action. I have a copy of the user manual that I will send out to you. Kind regards Richard Vernon Strategic Product Manager - Sectioning Products Anatomical Pathology Division Thermo Fisher Scientific Tudor Road, Manor Park, Runcorn, Cheshire, WA7 1TA, UK Tel:+44 (0) 1928 534122 | Mobile:+44 (0) 7825 119070 richard.vernon@thermofisher.com | http//:www.thermoscientific.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 10 November 2014 18:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. tendon help (Sanders, Jeanine (CDC/OID/NCEZID)) 2. Microm 335E Retraction (Stoll, Kathryn) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Nov 2014 17:05:06 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] tendon help To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <3B2CD438E1628A41BD687E98B963B7811FE5238A@EMBX-CHAM2.cdc.gov> Content-Type: text/plain; charset="us-ascii" Morning everyone, Can you please send me your best techniques, including processing schedules, for human tendon? Thanks, Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov ------------------------------ Message: 2 Date: Mon, 10 Nov 2014 17:11:03 +0000 From: "Stoll, Kathryn" Subject: [Histonet] Microm 335E Retraction To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Milwaukee WI 53226 Phone: 414-805-1525 Fax: 414-805-1528 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 11 ***************************************** From ian.bernard <@t> comcast.net Tue Nov 11 07:24:09 2014 From: ian.bernard <@t> comcast.net (ian bernard) Date: Tue Nov 11 07:24:27 2014 Subject: [Histonet] Validations- Retrospective, Concurrent and Prospective and Revalidations Message-ID: Validation experts: are there any NSH , CAP and or other Histo reference or literature that I can reference on the above types of validations? In our lab we will be using a some or a combination or all of these to comply with CAP validations standards of equipment, methods, protocols and key processes. Need guidance on the separate methods. V/r Ian R. Bernard, MSHA, HT (ASCP) HTL (Pend-2014) USAF, MSgt (Retired) Anatomic Pathology Technical Supervisor 10th Medical Group 210-687-7540 Cell ian.bernard@comcast.net ian.bernard.3@us.af.mil From kdwyer3322 <@t> aol.com Tue Nov 11 08:35:31 2014 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Tue Nov 11 08:35:36 2014 Subject: [Histonet] Texas Society for Histotechnology 2015 Sumposium/Convention - Plano, Texas Message-ID: <8D1CBD3E165CA1A-908-94DB@webmail-va122.sysops.aol.com> Hi Histonet, The Texas Society for Histotechnology is soliciting speakers for the 2015 Symposium/Convention. The Meeting is March 20-22, 2015, in Plano Texas. We need workshops in Histologic techniques, IHC, Molecular, Regulations, Safety, Quality Management, Telepathology, Forensics and Veterinary. If you are interested or know someone who would be interested please request a Call for Abstract via this e-mail. Deadline for abstracts is November 30, 2014. If you have any questions please contact me at 214-980-4960. Thanks, Kathy Dwyer TSH Convention Coordinator From richardb <@t> omsc.net Tue Nov 11 11:48:53 2014 From: richardb <@t> omsc.net (Richard Bowker) Date: Tue Nov 11 11:49:41 2014 Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 10 In-Reply-To: <7c5d7eec-9eec-450d-8a98-5dedeeff318c@OMCMAIL.omsc.net> References: <7c5d7eec-9eec-450d-8a98-5dedeeff318c@OMCMAIL.omsc.net> Message-ID: <98d253e5f866412197f0045122714ca6@OMCMAIL.omsc.net> Judith, When you pick up your sections are you getting a lot of water trapped between the tissue and the slides. I have had that problem when getting a new lot number of slides before and had to change them out. The tissue wasn't actuall falling off rather then being pushed off after the water begins to heat up and expand forcing the tissue off. In this instance shaking the slide after picking up the tissue had no benifit either as this also caused the water to force the tissue off the slides. Rick Bowker Histology supervisor Olympia Multi-Specialty Clinic richardb@omsc.net Message: 5 Date: Sat, 8 Nov 2014 13:31:27 +0300 From: "Jamal" Subject: RE: [Histonet] Tissue falling off positive slides To: "'Pardue, Judith'" , Message-ID: <00c001cffb3f$297ea1f0$7c7be5d0$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="windows-1256" Do you use Autostainer? What brand ? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Thursday, November 06, 2014 6:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue falling off positive slides WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 Judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sruby <@t> 4path.com Tue Nov 11 12:09:15 2014 From: Sruby <@t> 4path.com (Stephen G. Ruby) Date: Tue Nov 11 12:09:22 2014 Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 12 In-Reply-To: References: Message-ID: Kathryn Here is a link to the owners manual http://www.bu.edu/becf/downloads/BioInterface%20Technologies/HM%20355S%20Manual.pdf Stephen G. Ruby, MD, MBA, FCAP President and Medical Director, 4path, Ltd. Value, Service and Commitment...Beyond the Diagnosis V: 1-877-884-7284 F: 708-929-4330 www.4path.com 9050 W. 81st Street, Justice, IL 60458 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, November 11, 2014 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://cp.mcafee.com/d/5fHCNEq41ASyMy-ejud7adPtPqtTAnNPVEV73zqtTAnNPBT4jqtTAnNPVEV76zASDtV5V5BBCVKAmO1o5cbrcDOVJxrpA-ndEntojsovW_8ILIcFZuVtdBVzATTbI8YJteOaqJT4-l3PWApmU6CQjqdPtPtPo0c-l9QVKtaJP9CfFfUMXQ6Y4RtxxYGjB1SK4VkDk6COsGjG3iV2Hsbvg57OFeDdPFlKpcNZ9_67uwSrhvKYUr2YGjG3jh07i8_io1Cy0cRfd42bao1Cy0dFhLPh0yCrpodGjcpla1SYkP8 or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CPT Code changes (Weems, Joyce K.) 2. Re: Microm 335E Retraction (Mike Andrews) 3. RE: Microm 335E Retraction (Vernon, Richard I.) 4. Validations- Retrospective, Concurrent and Prospective and Revalidations (ian bernard) 5. Texas Society for Histotechnology 2015 Sumposium/Convention - Plano, Texas (kdwyer3322@aol.com) 6. RE: Histonet Digest, Vol 132, Issue 10 (Richard Bowker) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Nov 2014 21:39:22 +0000 From: "Weems, Joyce K." Subject: [Histonet] CPT Code changes To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Have you all seen this news? "To eliminate these discrepancies, CPT(r) 2015 revises parent code 88342, imunohistochemistry or immunocytochemistry, per specimen; initial single antibody stain procedure, deletes indented code 88343, and adds two new indented codes, +88341, each additional single antibody stain procedure, and 88344, each multiplex antibody stain procedure." Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org http://cp.mcafee.com/d/1jWVIp3zqb2bUVdUQsETdTdFTuhv7fCzAsedFTuhv7enshdFTuhv7fCzAsqejqtTAnAmmmrCWhr85wkMJIOvbCS5JCjVsSxtRxdNx_HYyO-MODRXBQSnCejvsKMzORQX8FGTsjVkffGhBrwqrjdETdTdTdBeFIEj4qQZoDjpDX2uY01dAVkDk6BO5mUm-wafBiterDiHsOpzWj-ceZ1ISy_tVMS5VkDk6Cy0eAh-AM3d40pGuq84mkM3d40rizvCy15cSOMrfk9d 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 2 Date: Mon, 10 Nov 2014 21:16:32 -0600 From: Mike Andrews Subject: Re: [Histonet] Microm 335E Retraction To: "Stoll, Kathryn" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <228C4E7F-1815-42E0-BFE5-519A92BFE008@gmail.com> Content-Type: text/plain; charset=us-ascii A complete user manual is available at http://cp.mcafee.com/d/5fHCN8g4zqb2bUVdUQsETdTdFTuhv7fCzAsedFTuhv7enshdFTuhv7fCzAsqejqtTAnAmmmrCWhr85wkMJIOvbCS5JCjVsSxtRxdNx_HYyO-MODRXBQSnCejvsKMzORQX8FGTsjVkffGhBrwqrpdETdTdTdw0y3vuwTx3XeUxkN0-KEkMpBkdxfUKXrNWtMjZysaDR8O-C1sr7FTpvv61WtNLodWvOO3FIDaAWwQKgGT2TQ1hYGjFPsWlrCjcvivNxTEdCQnXLe6MLaAWwQQg1QyfQC0pEw3djPh0yOC0pEw3qkrYQg8FCSm3rhUZ Mike Andrews, W5EGO WWME Oklahoma area executive team > On Nov 10, 2014, at 11:11 AM, Stoll, Kathryn wrote: > > I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? > > Kathryn Stoll > Supervisor Histology > Clinical and Translational Research Core Lab Medical College of > Wisconsin > 9200 W. Wisconsin Ave Room 1176 > Milwaukee WI 53226 > Phone: 414-805-1525 > Fax: 414-805-1528 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://cp.mcafee.com/d/k-Kr4x8SyMy-ejud7adPtPqtTAnNPVEV73zqtTAnNPBT4jq > tTAnNPVEV76zASDtV5V5BBCVKAmO1o5cbrcDOVJxrpA-ndEntojsovW_8ILIcFZuVtdBVz > ATTbI8YJteOaqJT4-l3PWApmU6CS3qdPtPtPo0c-l9QVKtaJP9CfFfUMXQ6Y4RtxxYGjB1 > SK4VkDk6COsGjG3iV2Hsbvg57OFeDdPFlKpcNZ9_67uwSrhvKYUr2YGjG3jh07i8_io1Cy > 0cRfd42bao1Cy0dFhLPh0yCrpodI3Qg ------------------------------ Message: 3 Date: Tue, 11 Nov 2014 07:37:57 +0000 From: "Vernon, Richard I." Subject: [Histonet] RE: Microm 335E Retraction To: "histonet@lists.utsouthwestern.edu" Message-ID: <8411B0BF0CE50F4EA179AB3A06DB5C03560F35EB50@UKHIG-MXVS01.emea.thermo.com> Content-Type: text/plain; charset="us-ascii" Dear Kathryn, The retraction on the HM355E can be turned off by simultaneously pressing the "A" button and the button to the left of this (with the double arrow on it). The retraction light (yellow LED) will then go off. If the light still remains on turn the hand wheel once and it should go off confirming your action. I have a copy of the user manual that I will send out to you. Kind regards Richard Vernon Strategic Product Manager - Sectioning Products Anatomical Pathology Division Thermo Fisher Scientific Tudor Road, Manor Park, Runcorn, Cheshire, WA7 1TA, UK Tel:+44 (0) 1928 534122 | Mobile:+44 (0) 7825 119070 richard.vernon@thermofisher.com | http//:http://cp.mcafee.com/d/k-Kr4zqb2bUVdUQsETdTdFTuhv7fCzAsedFTuhv7enshdFTuhv7fCzAsqejqtTAnAmmmrCWhr85wkMJIOvbCS5JCjVsSxtRxdNx_HYyO-MODRXBQSnCejvsKMzORQX8FGTsjVkffGhBrwqrvdETdTdTdBeCj-aJqmBY9OtBrBPpel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdELTusdxul9R1FEw3F4vFc0Ph06qDCy15Bc0Ph06QETVEwhjdII6XfSgzgApLqTN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 10 November 2014 18:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://cp.mcafee.com/d/k-Kr6h8SyMy-ejud7adPtPqtTAnNPVEV73zqtTAnNPBT4jqtTAnNPVEV76zASDtV5V5BBCVKAmO1o5cbrcDOVJxrpA-ndEntojsovW_8ILIcFZuVtdBVzATTbI8YJteOaqJT4-l3PWApmU6CT3qdPtPtPo0c-l9QVKtaJP9CfFfUMXQ6Y4RtxxYGjB1SK4VkDk6COsGjG3iV2Hsbvg57OFeDdPFlKpcNZ9_67uwSrhvKYUr2YGjG3jh07i8_io1Cy0cRfd42bao1Cy0dFhLPh0yCrpodLM2itXta or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. tendon help (Sanders, Jeanine (CDC/OID/NCEZID)) 2. Microm 335E Retraction (Stoll, Kathryn) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Nov 2014 17:05:06 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] tendon help To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <3B2CD438E1628A41BD687E98B963B7811FE5238A@EMBX-CHAM2.cdc.gov> Content-Type: text/plain; charset="us-ascii" Morning everyone, Can you please send me your best techniques, including processing schedules, for human tendon? Thanks, Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov ------------------------------ Message: 2 Date: Mon, 10 Nov 2014 17:11:03 +0000 From: "Stoll, Kathryn" Subject: [Histonet] Microm 335E Retraction To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Milwaukee WI 53226 Phone: 414-805-1525 Fax: 414-805-1528 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/avndzgO73hJ5x5YsCYqekrCXCQXL8LzDPhOe76QXL8LzDbK8CQXL8LzDPhOed79JeXObObbbdPt8JA2MaomSpfBPr2SP9YKrgKWMCUM_R-hpvopjWZOWrbP79LKnohVqWtAkRrK9YG7DR8OJMddzzqdPtPtPo0c-l9QVKtaJP9CfFfUMXQ6Y4RtxxYGjB1SK4VkDk6COsGjG3iV2Hsbvg57OFeDdPFlKpcNZ9_67uwSrhvKYUr2YGjG3jh07i8_io1Cy0cRfd42bao1Cy0dFhLPh0yCrpodTpanv73xgrPb End of Histonet Digest, Vol 132, Issue 11 ***************************************** ------------------------------ Message: 4 Date: Tue, 11 Nov 2014 06:24:09 -0700 From: "ian bernard" Subject: [Histonet] Validations- Retrospective, Concurrent and Prospective and Revalidations To: Message-ID: Content-Type: text/plain; charset="us-ascii" Validation experts: are there any NSH , CAP and or other Histo reference or literature that I can reference on the above types of validations? In our lab we will be using a some or a combination or all of these to comply with CAP validations standards of equipment, methods, protocols and key processes. Need guidance on the separate methods. V/r Ian R. Bernard, MSHA, HT (ASCP) HTL (Pend-2014) USAF, MSgt (Retired) Anatomic Pathology Technical Supervisor 10th Medical Group 210-687-7540 Cell ian.bernard@comcast.net ian.bernard.3@us.af.mil ------------------------------ Message: 5 Date: Tue, 11 Nov 2014 09:35:31 -0500 From: kdwyer3322@aol.com Subject: [Histonet] Texas Society for Histotechnology 2015 Sumposium/Convention - Plano, Texas To: histonet@lists.utsouthwestern.edu Message-ID: <8D1CBD3E165CA1A-908-94DB@webmail-va122.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hi Histonet, The Texas Society for Histotechnology is soliciting speakers for the 2015 Symposium/Convention. The Meeting is March 20-22, 2015, in Plano Texas. We need workshops in Histologic techniques, IHC, Molecular, Regulations, Safety, Quality Management, Telepathology, Forensics and Veterinary. If you are interested or know someone who would be interested please request a Call for Abstract via this e-mail. Deadline for abstracts is November 30, 2014. If you have any questions please contact me at 214-980-4960. Thanks, Kathy Dwyer TSH Convention Coordinator ------------------------------ Message: 6 Date: Tue, 11 Nov 2014 17:48:53 +0000 From: Richard Bowker Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 10 To: "histonet@lists.utsouthwestern.edu" Message-ID: <98d253e5f866412197f0045122714ca6@OMCMAIL.omsc.net> Content-Type: text/plain; charset="us-ascii" Judith, When you pick up your sections are you getting a lot of water trapped between the tissue and the slides. I have had that problem when getting a new lot number of slides before and had to change them out. The tissue wasn't actuall falling off rather then being pushed off after the water begins to heat up and expand forcing the tissue off. In this instance shaking the slide after picking up the tissue had no benifit either as this also caused the water to force the tissue off the slides. Rick Bowker Histology supervisor Olympia Multi-Specialty Clinic richardb@omsc.net Message: 5 Date: Sat, 8 Nov 2014 13:31:27 +0300 From: "Jamal" Subject: RE: [Histonet] Tissue falling off positive slides To: "'Pardue, Judith'" , Message-ID: <00c001cffb3f$297ea1f0$7c7be5d0$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="windows-1256" Do you use Autostainer? What brand ? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://cp.mcafee.com/d/1jWVIg6hEpdEI8LzATzhOzsTsSDtV5Ys-qehMUSDtV5YsVtN4SDtV5Ys-qehNEVdFTuhuhpppKrF5Iwm1j2SP9YKromSpfBPq5Tm4T67-LObbX3avnKnjpuoVdZOX2fbnjIyCHtNfBgY-F6lK1FJUSzsTsTsSkXjO004_i5oXeIpvjBPpel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdELTusdxul9R1FEw3F4vFc0Ph06qDCy15Bc0Ph06QETVEwhjdII6OdmyC7Fq -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Thursday, November 06, 2014 6:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue falling off positive slides WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 Judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/k-Kr3x0g3zqb2bUVdUQsETdTdFTuhv7fCzAsedFTuhv7enshdFTuhv7fCzAsqejqtTAnAmmmrCWhr85wkMJIOvbCS5JCjVsSxtRxdNx_HYyO-MODRXBQSnCejvsKMzORQX8FGTsjVkffGhBrwqrd79J6VKVKVI06vaAWsTeBmVAP7QDYotW3u2qKMM-l9OwXn2sGjG3jpel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdELTusdxul9R1FEw3F4vFc0Ph06qDCy15Bc0Ph06QETVEwhjdII6ZOWN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://cp.mcafee.com/d/avndz8Ad1MArhohv79L6zB6VKVJeXObUVYQszxNJeXObUVOXy9JeXObUVYQszzhOrjKYyYyOOPsTibp0I2C5JCjVsSMJIOvbCQbKI9KcfZvAmnS6k-LsKCOYNOrXBS4umKDp5dmXyvaxVZicHs3jqdPqdPtPtPo0c-l9QVKtaJP9CfFfUMXQ6Y4RtxxYGjB1SK4VkDk6COsGjG3iV2Hsbvg57OFeDdPFlKpcNZ9_67uwSrhvKYUr2YGjG3jh07i8_io1Cy0cRfd42bao1Cy0dFhLPh0yCrpodVOa4o0geG8WA End of Histonet Digest, Vol 132, Issue 12 ***************************************** From mjones <@t> metropath.com Tue Nov 11 12:15:31 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Nov 11 12:15:38 2014 Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 10 Message-ID: We use the platinum series from Mercedes Medical - they are so hydrophilic, that if you place your section wrong on the slide, you cannot float it off and start over. We also take our sections at a backwards angle from the surface of the water: rather than lift from underneath - we put the slide in the water bath next to the section face down at a severe angle, attach the section to the top of the slide (or wherever) and then pull slide out at angle. This encourages the water to stay in the bath and not under sections. Does that make sense? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 11/11/14, 10:48 AM, "Richard Bowker" wrote: >Judith, > >When you pick up your sections are you getting a lot of water trapped >between the tissue and the slides. I have had that problem when getting a >new lot number of slides before and had to change them out. The tissue >wasn't actuall falling off rather then being pushed off after the water >begins to heat up and expand forcing the tissue off. In this instance >shaking the slide after picking up the tissue had no benifit either as >this also caused the water to force the tissue off the slides. > >Rick Bowker >Histology supervisor >Olympia Multi-Specialty Clinic >richardb@omsc.net > >Message: 5 >Date: Sat, 8 Nov 2014 13:31:27 +0300 >From: "Jamal" >Subject: RE: [Histonet] Tissue falling off positive slides >To: "'Pardue, Judith'" , > >Message-ID: > <00c001cffb3f$297ea1f0$7c7be5d0$@rowaihi@alborglaboratories.com> >Content-Type: text/plain; charset="windows-1256" > >Do you use Autostainer? >What brand ? > > >Best Regards, > > >Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg >Medical Laboratories |? Mobile +966 503629832| >j.rowaihi@alborglaboratories.com >Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | >Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | >www.alborglaboratories.com > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, >Judith >Sent: Thursday, November 06, 2014 6:38 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Tissue falling off positive slides > >WE are having trouble with tissue coming off our h&e slides. Our heater is >set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue >positive slides. > >Judith Pardue >CHI Memorial >Chatt. Tn. 37343 >Judith_pardue@memorial.org > >This electronic mail and any attached documents are intended solely for >the >named addressee(s) and contain confidential information. If you are not an >addressee, or responsible for delivering this email to an addressee, you >have received this email in error and are notified that reading, copying, >or >disclosing this email is prohibited. If you received this email in error, >immediately reply to the sender and delete the message completely from >your >computer system. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From esulkosky <@t> gmail.com Tue Nov 11 14:35:23 2014 From: esulkosky <@t> gmail.com (Eric Sulkosky) Date: Tue Nov 11 14:35:31 2014 Subject: [Histonet] Histology Professional's wanted in Pittsburgh, PA Message-ID: <34C43493-81EF-41B8-A008-8143F310FADB@gmail.com> Hello Histonet, I would like to tell you about a great opportunity that is now available at Dermpath Diagnostics- A division of Quest Diagnostics. The laboratory is now under new management and we are currently recruiting for a FT (Job ID 3735022) and Per Diem (Job ID 3734228) Histology professional. If interested, you can apply for the position(s) on the Quest Diagnostics website. http://www.questdiagnostics.com/home/about/careers/job-search.html Dermpath Diagnostics is exclusively focused on providing exceptional dermatopathology services. Our commitment to dermatopathology is backed by an unrivaled team of over 75 board-certified dermatopathologists, advanced diagnostic technologies and a proficient support team dedicated to serve you, your staff and patients. Our mission is to provide accurate, clear and prompt diagnoses. Through the development of strong consultative relationships with each of our referring clinicians, together we will provide the best possible care for every patient. We look forward to hearing from you soon. From khbarr <@t> mdanderson.org Tue Nov 11 15:43:52 2014 From: khbarr <@t> mdanderson.org (Barr,Kaye H) Date: Tue Nov 11 15:44:03 2014 Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 12 In-Reply-To: <49e5dcf1-d6ec-4187-91aa-d5323c840f1a@DCPWPEXHUBCAS02.mdanderson.edu> References: <49e5dcf1-d6ec-4187-91aa-d5323c840f1a@DCPWPEXHUBCAS02.mdanderson.edu> Message-ID: <65C8EC869E8581459DCD566079572FAC27666D41@DCPWPEXMBX02.mdanderson.edu> Does anyone do a safety inservice for lab personnel regarding cuts/lacerations received when performing microtomy (paraffin & FS)? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, November 11, 2014 12:52 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CPT Code changes (Weems, Joyce K.) 2. Re: Microm 335E Retraction (Mike Andrews) 3. RE: Microm 335E Retraction (Vernon, Richard I.) 4. Validations- Retrospective, Concurrent and Prospective and Revalidations (ian bernard) 5. Texas Society for Histotechnology 2015 Sumposium/Convention - Plano, Texas (kdwyer3322@aol.com) 6. RE: Histonet Digest, Vol 132, Issue 10 (Richard Bowker) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Nov 2014 21:39:22 +0000 From: "Weems, Joyce K." Subject: [Histonet] CPT Code changes To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Have you all seen this news? "To eliminate these discrepancies, CPT(r) 2015 revises parent code 88342, imunohistochemistry or immunocytochemistry, per specimen; initial single antibody stain procedure, deletes indented code 88343, and adds two new indented codes, +88341, each additional single antibody stain procedure, and 88344, each multiplex antibody stain procedure." Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 2 Date: Mon, 10 Nov 2014 21:16:32 -0600 From: Mike Andrews Subject: Re: [Histonet] Microm 335E Retraction To: "Stoll, Kathryn" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <228C4E7F-1815-42E0-BFE5-519A92BFE008@gmail.com> Content-Type: text/plain; charset=us-ascii A complete user manual is available at http://www.bu.edu/becf/downloads/BioInterface%20Technologies/HM%20355S%20Manual.pdf Mike Andrews, W5EGO WWME Oklahoma area executive team > On Nov 10, 2014, at 11:11 AM, Stoll, Kathryn wrote: > > I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? > > Kathryn Stoll > Supervisor Histology > Clinical and Translational Research Core Lab Medical College of > Wisconsin > 9200 W. Wisconsin Ave Room 1176 > Milwaukee WI 53226 > Phone: 414-805-1525 > Fax: 414-805-1528 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 11 Nov 2014 07:37:57 +0000 From: "Vernon, Richard I." Subject: [Histonet] RE: Microm 335E Retraction To: "histonet@lists.utsouthwestern.edu" Message-ID: <8411B0BF0CE50F4EA179AB3A06DB5C03560F35EB50@UKHIG-MXVS01.emea.thermo.com> Content-Type: text/plain; charset="us-ascii" Dear Kathryn, The retraction on the HM355E can be turned off by simultaneously pressing the "A" button and the button to the left of this (with the double arrow on it). The retraction light (yellow LED) will then go off. If the light still remains on turn the hand wheel once and it should go off confirming your action. I have a copy of the user manual that I will send out to you. Kind regards Richard Vernon Strategic Product Manager - Sectioning Products Anatomical Pathology Division Thermo Fisher Scientific Tudor Road, Manor Park, Runcorn, Cheshire, WA7 1TA, UK Tel:+44 (0) 1928 534122 | Mobile:+44 (0) 7825 119070 richard.vernon@thermofisher.com | http//:www.thermoscientific.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 10 November 2014 18:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. tendon help (Sanders, Jeanine (CDC/OID/NCEZID)) 2. Microm 335E Retraction (Stoll, Kathryn) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Nov 2014 17:05:06 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] tendon help To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <3B2CD438E1628A41BD687E98B963B7811FE5238A@EMBX-CHAM2.cdc.gov> Content-Type: text/plain; charset="us-ascii" Morning everyone, Can you please send me your best techniques, including processing schedules, for human tendon? Thanks, Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov ------------------------------ Message: 2 Date: Mon, 10 Nov 2014 17:11:03 +0000 From: "Stoll, Kathryn" Subject: [Histonet] Microm 335E Retraction To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Milwaukee WI 53226 Phone: 414-805-1525 Fax: 414-805-1528 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 11 ***************************************** ------------------------------ Message: 4 Date: Tue, 11 Nov 2014 06:24:09 -0700 From: "ian bernard" Subject: [Histonet] Validations- Retrospective, Concurrent and Prospective and Revalidations To: Message-ID: Content-Type: text/plain; charset="us-ascii" Validation experts: are there any NSH , CAP and or other Histo reference or literature that I can reference on the above types of validations? In our lab we will be using a some or a combination or all of these to comply with CAP validations standards of equipment, methods, protocols and key processes. Need guidance on the separate methods. V/r Ian R. Bernard, MSHA, HT (ASCP) HTL (Pend-2014) USAF, MSgt (Retired) Anatomic Pathology Technical Supervisor 10th Medical Group 210-687-7540 Cell ian.bernard@comcast.net ian.bernard.3@us.af.mil ------------------------------ Message: 5 Date: Tue, 11 Nov 2014 09:35:31 -0500 From: kdwyer3322@aol.com Subject: [Histonet] Texas Society for Histotechnology 2015 Sumposium/Convention - Plano, Texas To: histonet@lists.utsouthwestern.edu Message-ID: <8D1CBD3E165CA1A-908-94DB@webmail-va122.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hi Histonet, The Texas Society for Histotechnology is soliciting speakers for the 2015 Symposium/Convention. The Meeting is March 20-22, 2015, in Plano Texas. We need workshops in Histologic techniques, IHC, Molecular, Regulations, Safety, Quality Management, Telepathology, Forensics and Veterinary. If you are interested or know someone who would be interested please request a Call for Abstract via this e-mail. Deadline for abstracts is November 30, 2014. If you have any questions please contact me at 214-980-4960. Thanks, Kathy Dwyer TSH Convention Coordinator ------------------------------ Message: 6 Date: Tue, 11 Nov 2014 17:48:53 +0000 From: Richard Bowker Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 10 To: "histonet@lists.utsouthwestern.edu" Message-ID: <98d253e5f866412197f0045122714ca6@OMCMAIL.omsc.net> Content-Type: text/plain; charset="us-ascii" Judith, When you pick up your sections are you getting a lot of water trapped between the tissue and the slides. I have had that problem when getting a new lot number of slides before and had to change them out. The tissue wasn't actuall falling off rather then being pushed off after the water begins to heat up and expand forcing the tissue off. In this instance shaking the slide after picking up the tissue had no benifit either as this also caused the water to force the tissue off the slides. Rick Bowker Histology supervisor Olympia Multi-Specialty Clinic richardb@omsc.net Message: 5 Date: Sat, 8 Nov 2014 13:31:27 +0300 From: "Jamal" Subject: RE: [Histonet] Tissue falling off positive slides To: "'Pardue, Judith'" , Message-ID: <00c001cffb3f$297ea1f0$7c7be5d0$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="windows-1256" Do you use Autostainer? What brand ? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Thursday, November 06, 2014 6:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue falling off positive slides WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 Judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 12 ***************************************** From Toni.Rathborne <@t> rwjuh.edu Tue Nov 11 15:51:56 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Tue Nov 11 15:52:06 2014 Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 12 In-Reply-To: <65C8EC869E8581459DCD566079572FAC27666D41@DCPWPEXMBX02.mdanderson.edu> References: <49e5dcf1-d6ec-4187-91aa-d5323c840f1a@DCPWPEXHUBCAS02.mdanderson.edu> <65C8EC869E8581459DCD566079572FAC27666D41@DCPWPEXMBX02.mdanderson.edu> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED9CB4@vap1014.win.rwjuh.edu> As part of reporting all work related injuries, we investigate the cause or contributing factor and determine if the injury could have been avoided, and how to do so in the future. These types of injuries happen infrequently, so we have not done regular in-services. It is a good idea though, and should be something to consider. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barr,Kaye H Sent: Tuesday, November 11, 2014 4:44 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 12 Does anyone do a safety inservice for lab personnel regarding cuts/lacerations received when performing microtomy (paraffin & FS)? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, November 11, 2014 12:52 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CPT Code changes (Weems, Joyce K.) 2. Re: Microm 335E Retraction (Mike Andrews) 3. RE: Microm 335E Retraction (Vernon, Richard I.) 4. Validations- Retrospective, Concurrent and Prospective and Revalidations (ian bernard) 5. Texas Society for Histotechnology 2015 Sumposium/Convention - Plano, Texas (kdwyer3322@aol.com) 6. RE: Histonet Digest, Vol 132, Issue 10 (Richard Bowker) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Nov 2014 21:39:22 +0000 From: "Weems, Joyce K." Subject: [Histonet] CPT Code changes To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Have you all seen this news? "To eliminate these discrepancies, CPT(r) 2015 revises parent code 88342, imunohistochemistry or immunocytochemistry, per specimen; initial single antibody stain procedure, deletes indented code 88343, and adds two new indented codes, +88341, each additional single antibody stain procedure, and 88344, each multiplex antibody stain procedure." Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 2 Date: Mon, 10 Nov 2014 21:16:32 -0600 From: Mike Andrews Subject: Re: [Histonet] Microm 335E Retraction To: "Stoll, Kathryn" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <228C4E7F-1815-42E0-BFE5-519A92BFE008@gmail.com> Content-Type: text/plain; charset=us-ascii A complete user manual is available at http://www.bu.edu/becf/downloads/BioInterface%20Technologies/HM%20355S%20Manual.pdf Mike Andrews, W5EGO WWME Oklahoma area executive team > On Nov 10, 2014, at 11:11 AM, Stoll, Kathryn wrote: > > I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? > > Kathryn Stoll > Supervisor Histology > Clinical and Translational Research Core Lab Medical College of > Wisconsin > 9200 W. Wisconsin Ave Room 1176 > Milwaukee WI 53226 > Phone: 414-805-1525 > Fax: 414-805-1528 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 11 Nov 2014 07:37:57 +0000 From: "Vernon, Richard I." Subject: [Histonet] RE: Microm 335E Retraction To: "histonet@lists.utsouthwestern.edu" Message-ID: <8411B0BF0CE50F4EA179AB3A06DB5C03560F35EB50@UKHIG-MXVS01.emea.thermo.com> Content-Type: text/plain; charset="us-ascii" Dear Kathryn, The retraction on the HM355E can be turned off by simultaneously pressing the "A" button and the button to the left of this (with the double arrow on it). The retraction light (yellow LED) will then go off. If the light still remains on turn the hand wheel once and it should go off confirming your action. I have a copy of the user manual that I will send out to you. Kind regards Richard Vernon Strategic Product Manager - Sectioning Products Anatomical Pathology Division Thermo Fisher Scientific Tudor Road, Manor Park, Runcorn, Cheshire, WA7 1TA, UK Tel:+44 (0) 1928 534122 | Mobile:+44 (0) 7825 119070 richard.vernon@thermofisher.com | http//:www.thermoscientific.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 10 November 2014 18:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. tendon help (Sanders, Jeanine (CDC/OID/NCEZID)) 2. Microm 335E Retraction (Stoll, Kathryn) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Nov 2014 17:05:06 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] tendon help To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <3B2CD438E1628A41BD687E98B963B7811FE5238A@EMBX-CHAM2.cdc.gov> Content-Type: text/plain; charset="us-ascii" Morning everyone, Can you please send me your best techniques, including processing schedules, for human tendon? Thanks, Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov ------------------------------ Message: 2 Date: Mon, 10 Nov 2014 17:11:03 +0000 From: "Stoll, Kathryn" Subject: [Histonet] Microm 335E Retraction To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I have been given a Microm 335E microtome. I do not have a user manual with it. Does anyone know how to turn the retraction on and off? Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Milwaukee WI 53226 Phone: 414-805-1525 Fax: 414-805-1528 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 11 ***************************************** ------------------------------ Message: 4 Date: Tue, 11 Nov 2014 06:24:09 -0700 From: "ian bernard" Subject: [Histonet] Validations- Retrospective, Concurrent and Prospective and Revalidations To: Message-ID: Content-Type: text/plain; charset="us-ascii" Validation experts: are there any NSH , CAP and or other Histo reference or literature that I can reference on the above types of validations? In our lab we will be using a some or a combination or all of these to comply with CAP validations standards of equipment, methods, protocols and key processes. Need guidance on the separate methods. V/r Ian R. Bernard, MSHA, HT (ASCP) HTL (Pend-2014) USAF, MSgt (Retired) Anatomic Pathology Technical Supervisor 10th Medical Group 210-687-7540 Cell ian.bernard@comcast.net ian.bernard.3@us.af.mil ------------------------------ Message: 5 Date: Tue, 11 Nov 2014 09:35:31 -0500 From: kdwyer3322@aol.com Subject: [Histonet] Texas Society for Histotechnology 2015 Sumposium/Convention - Plano, Texas To: histonet@lists.utsouthwestern.edu Message-ID: <8D1CBD3E165CA1A-908-94DB@webmail-va122.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hi Histonet, The Texas Society for Histotechnology is soliciting speakers for the 2015 Symposium/Convention. The Meeting is March 20-22, 2015, in Plano Texas. We need workshops in Histologic techniques, IHC, Molecular, Regulations, Safety, Quality Management, Telepathology, Forensics and Veterinary. If you are interested or know someone who would be interested please request a Call for Abstract via this e-mail. Deadline for abstracts is November 30, 2014. If you have any questions please contact me at 214-980-4960. Thanks, Kathy Dwyer TSH Convention Coordinator ------------------------------ Message: 6 Date: Tue, 11 Nov 2014 17:48:53 +0000 From: Richard Bowker Subject: [Histonet] RE: Histonet Digest, Vol 132, Issue 10 To: "histonet@lists.utsouthwestern.edu" Message-ID: <98d253e5f866412197f0045122714ca6@OMCMAIL.omsc.net> Content-Type: text/plain; charset="us-ascii" Judith, When you pick up your sections are you getting a lot of water trapped between the tissue and the slides. I have had that problem when getting a new lot number of slides before and had to change them out. The tissue wasn't actuall falling off rather then being pushed off after the water begins to heat up and expand forcing the tissue off. In this instance shaking the slide after picking up the tissue had no benifit either as this also caused the water to force the tissue off the slides. Rick Bowker Histology supervisor Olympia Multi-Specialty Clinic richardb@omsc.net Message: 5 Date: Sat, 8 Nov 2014 13:31:27 +0300 From: "Jamal" Subject: RE: [Histonet] Tissue falling off positive slides To: "'Pardue, Judith'" , Message-ID: <00c001cffb3f$297ea1f0$7c7be5d0$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="windows-1256" Do you use Autostainer? What brand ? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Thursday, November 06, 2014 6:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue falling off positive slides WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 Judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 12 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne <@t> rwjuh.edu Tue Nov 11 15:58:44 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Tue Nov 11 15:58:50 2014 Subject: [Histonet] RE: CPT Code changes In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24ED9CF7@vap1014.win.rwjuh.edu> Where can we find more information on this? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, November 10, 2014 4:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT Code changes Have you all seen this news? "To eliminate these discrepancies, CPT(r) 2015 revises parent code 88342, imunohistochemistry or immunocytochemistry, per specimen; initial single antibody stain procedure, deletes indented code 88343, and adds two new indented codes, +88341, each additional single antibody stain procedure, and 88344, each multiplex antibody stain procedure." Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Tue Nov 11 16:11:23 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Tue Nov 11 16:11:29 2014 Subject: [Histonet] Question - flow cytometry Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355EC0B5@HHCEXCHMB03.hhcsystem.org> Does anyone have a protocol to "hold" specimens for flow cytometry until the pathologist examines a slide to confirm that it is "lymphoproliferative/lymphoma"? We seem to be doing a lot of flow on non-lymphoid specimens these days. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From igor.deyneko <@t> gmail.com Tue Nov 11 16:42:29 2014 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Tue Nov 11 16:42:33 2014 Subject: [Histonet] Part/Full time positions in Boston Message-ID: Dear Histonetters, I am looking for either part or full time histologist position in Boston/Cambridge ,MA areas. I have 8 years of lab experience working for 2 mid-size pharma companies. I started off in In-Vivo group and transitioned to strating up a histology core lab from scratch which slowly morphed into a Molecular Pathology dicision. Skills: - Carried out routine staff training on state of the art equipment and innovative staining methods - Performed tissue processing, paraffin and OCT embedding, microtome and cryotome sectioning - Executed standard histochemical, IHC and IF staining - Successfully streamlined procedures for biomarker screens of human and animal tissue samples - Implemented specialty staining procedures as well as a novel Beta-Gal staining method on frozen tissues - Performed qualitative and quantitative image analysis using Aperio ePathology and TissueGnostics modules jn I would appreciate any leads. T Thank you very much, I Igor Deyneko From j.benavides <@t> eae.csic.es Wed Nov 12 04:34:39 2014 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Wed Nov 12 04:39:04 2014 Subject: [Histonet] Keep cytology in the fridge Message-ID: <546337BF.3010809@eae.csic.es> Hi, Do you know for how long fixed cervical al smears can be kept in the fridge before staining (papanicolaou and HE) without any loss of slide quality? This is for a veterinary research project. We are sampling 23 cows a week for 6 weeks and I would rather stain them all at the same time (i.e. at the end of the project) than doing it during the project (we would be busy with lots of other things). Can I keep them, after fixing in ethanol, at 4? for 6 weeks? Thanks a lot for your help Julio From relia1 <@t> earthlink.net Wed Nov 12 11:18:03 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Nov 12 11:18:14 2014 Subject: [Histonet] RELIA Histology Careers Bulletin 11-14-2014 Message-ID: <00e701cffe9c$9df1da60$d9d58f20$@earthlink.net> Hi Histonetters!! I hope you are having a great day. Here is a quick post on the positions we are currently working on at RELIA Solutions. Some of these are new and some of them have updated information so please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral rewards! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives. MOST of these offer Sign- On Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments. The great thing about these opportunities is that if you are ready to move right away they are too and if you want to pursue a position with a start date after the holidays that works as well!! If You Or Anyone You Know Might Be Interested In Any Of These Positions Please Contact Me. You can reach me by email at relia1@earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. Here are the exciting opportunities I am talking about: Histology Supervisor - Long Island, NY - NYS lic NOT req Histology Supervisor - Northern AZ Lead Histotech - Belleville, IL Histology Tech - Kingsport, TN Histotech - Boulder, CO Histotech - Rapid City, SD Histotechnician - Hammond, IN Histology Tech - Williamsburg, VA 15K sign on bonus Grossing Histotechnologist - Philadelphia, PA Histotechnician - Modesto, CA Histotechnologist II - Northern AZ Have a great day. I look forward to hearing back from you. Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Dennis.Hahn <@t> cookchildrens.org Wed Nov 12 11:34:38 2014 From: Dennis.Hahn <@t> cookchildrens.org (Dennis Hahn) Date: Wed Nov 12 11:34:43 2014 Subject: [Histonet] Open PA Position Message-ID: Cook Children's Medical Center in Ft Worth, TX has an immediate opening for a Pathologist Assistant. Requires PA(ASCP). Cook Children's Medical Center is ranked in the top 7 percent of the nation's hospitals for its nursing excellence and has a distinguished reputation for its extraordinary care and outcomes. The 429-bed Medical Center is the cornerstone of Cook Children's Health Care System that serves most of North and West Texas, and beyond. This position works closely with the Pathologists, Histology, Laboratory, Research and Surgery personnel on a daily basis to ensure routine, research and postmortem specimens are optimally processed in our ever growing Histology Laboratory. Our new Laboratory is scheduled to open in early Spring, 2016. Interested applicants must work well in a small team environment, while being highly motivated to accomplish many tasks independently to meet Laboratory, Medical Center, CAP and JCAHO guidelines. Applicants may contact me for more information, but must apply online at www.cookchildrens.org. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 From pruegghm <@t> hotmail.com Wed Nov 12 11:35:15 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Wed Nov 12 11:35:20 2014 Subject: [Histonet] TC nuclear stain Message-ID: TC experts. One of my former students is doing a TC stain and the nuclear stain with iron hematoxylin is washing out. I remember that I had this problem myself with weigerts/iron heme, I always got a weaker purple stain rather than black like it was supposed to be. Do you think it would help to repeat the nuclear stain at the end of the stain as well as in the beginning, make fresh hematoxylin, etc???? Thank you for your advise, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From b427297 <@t> aol.com Wed Nov 12 11:55:35 2014 From: b427297 <@t> aol.com (William J. O'Connor III) Date: Wed Nov 12 11:55:41 2014 Subject: [Histonet] IHC general fixation Message-ID: <8D1CCB8FE37681B-14F0-10276@webmail-vm014.sysops.aol.com> I'm looking for input on the latest theories of duration of fixation for routine IHC. In the past, it was thought that tissues should be removed from 10% NBF after a max of 72 hours, and if not immediately processed, transferred to 70% ethanol until processed to retain antigenicity. What is the current thinking/trends? Over the last couple of years, I have heard that leaving tissues in 70% ethanol may be doing more harm than good, and it's NOT a good idea to leave them in alcohol for extended periods of time. I would appreciate your input, or point me towards any new data. Jackie O' From Timothy.Morken <@t> ucsf.edu Wed Nov 12 12:55:20 2014 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Nov 12 12:56:34 2014 Subject: [Histonet] IHC general fixation In-Reply-To: <8D1CCB8FE37681B-14F0-10276@webmail-vm014.sysops.aol.com> References: <8D1CCB8FE37681B-14F0-10276@webmail-vm014.sysops.aol.com> Message-ID: <761E2B5697F795489C8710BCC72141FF367B6285@ex07.net.ucsf.edu> In general, since the advent of heat-mediated antigen retrieval long fixation time is not an issue. In fact there are more problems associated with short fixation times (less than 6 hours) and IHC, than long fixation times. Studies have shown that even tissue left in formalin for weeks, months, even a year, will have good antigenicity with heat antigen retrieval. The key for good reproducibility is to make your fixation times consistent, not wildly different from day to day and embed rather than keeping in other solutions like alcohol. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William J. O'Connor III Sent: Wednesday, November 12, 2014 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC general fixation I'm looking for input on the latest theories of duration of fixation for routine IHC. In the past, it was thought that tissues should be removed from 10% NBF after a max of 72 hours, and if not immediately processed, transferred to 70% ethanol until processed to retain antigenicity. What is the current thinking/trends? Over the last couple of years, I have heard that leaving tissues in 70% ethanol may be doing more harm than good, and it's NOT a good idea to leave them in alcohol for extended periods of time. I would appreciate your input, or point me towards any new data. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcohoon00 <@t> msn.com Wed Nov 12 13:02:50 2014 From: jcohoon00 <@t> msn.com (JOHNNY COHOON) Date: Wed Nov 12 13:02:58 2014 Subject: [Histonet] Open position Mohs tech Message-ID: We are looking for a histotech in the Fort Worth Texas area. If you are interested please send your resume or inquiries to heather.drwalsh@yahoo.com Johnny Cohoon Please Consider the environment before printing this e-mail. From Mark.Elliott <@t> hli.ubc.ca Wed Nov 12 16:31:52 2014 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Wed Nov 12 16:32:03 2014 Subject: [Histonet] monomeric myosin antibody for paraffin Message-ID: <54636F58020000D6000772FD@mail.hli.ubc.ca> Does anyone know of a source for an antibody to monomeric myosin that will work on human tissues? We have looked but haven't been able to find one. Any suggestions would be greatly appreciated. Thanks Mark From j.rowaihi <@t> alborglaboratories.com Thu Nov 13 01:34:30 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Thu Nov 13 01:34:12 2014 Subject: [Histonet] IHC general fixation In-Reply-To: <8D1CCB8FE37681B-14F0-10276@webmail-vm014.sysops.aol.com> References: <8D1CCB8FE37681B-14F0-10276@webmail-vm014.sysops.aol.com> Message-ID: <00f601cfff14$444512c0$cccf3840$@rowaihi@alborglaboratories.com> Good day I recommend to see below study http://biospecimens.cancer.gov/meeting/brnsymposium/docs/Grizzle.pdf Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William J. O'Connor III Sent: Wednesday, November 12, 2014 8:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC general fixation I'm looking for input on the latest theories of duration of fixation for routine IHC. In the past, it was thought that tissues should be removed from 10% NBF after a max of 72 hours, and if not immediately processed, transferred to 70% ethanol until processed to retain antigenicity. What is the current thinking/trends? Over the last couple of years, I have heard that leaving tissues in 70% ethanol may be doing more harm than good, and it's NOT a good idea to leave them in alcohol for extended periods of time. I would appreciate your input, or point me towards any new data. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lori.Disher <@t> HCAhealthcare.com Thu Nov 13 11:04:17 2014 From: Lori.Disher <@t> HCAhealthcare.com (Lori.Disher@HCAhealthcare.com) Date: Thu Nov 13 11:04:51 2014 Subject: [Histonet] Processed blocks before decaling Message-ID: <778DD853CF606049A37FC2059C8BA07A9EFB8EB725@FWDCWPMSGCMS04.hca.corpad.net> Hello, We had several tissue cassettes put on the processor before they were decaled. Can I just melt the blocks, run thru xylene, alcohol & water. Put in decal and re-process? Thanks in advance for your expert advice! Lori Disher Lori.disher@hcahealthcare.com From klaus.dern44 <@t> gmail.com Thu Nov 13 11:27:31 2014 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Thu Nov 13 11:27:35 2014 Subject: [Histonet] THICK AND THIN SECTIONS ? Message-ID: If you are using one of the below mentioned microtomes and the advance mechanism is worn out ( too much play between spindle and spindle nut ), you could be faced with purchasing a new microtome. REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1800 Cryostat SAKURA - SRM 200 These models are no longer supported by the manufacturer, ( no parts availability ). Rather than replacing these excellent instruments, I have a PERMANENT solution to fix this problem. For information please contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com From rjbuesa <@t> yahoo.com Thu Nov 13 11:33:35 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 13 11:33:40 2014 Subject: [Histonet] Processed blocks before decaling In-Reply-To: <778DD853CF606049A37FC2059C8BA07A9EFB8EB725@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A9EFB8EB725@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: <1471185785.303656.1415900015642.JavaMail.yahoo@jws10073.mail.ne1.yahoo.com> Yes, but you have to be sure you have eliminated ALL the paraffin!Ren? J.? On Thursday, November 13, 2014 12:06 PM, "Lori.Disher@HCAhealthcare.com" wrote: Hello, We had several tissue cassettes put on the processor before they were decaled.? Can I just melt the blocks, run thru xylene, alcohol & water.? Put in decal and re-process? Thanks in advance for your expert advice! Lori Disher Lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ksverlow <@t> cahfs.ucdavis.edu Thu Nov 13 14:07:41 2014 From: ksverlow <@t> cahfs.ucdavis.edu (Sverlow, Karen) Date: Thu Nov 13 14:08:25 2014 Subject: [Histonet] IHC apoptosis marker (caspase 3 or similar) Message-ID: <76CA4EC69CB72043944BA109DDE043B80C2E48B0@Exchange.cahfs.ucdavis.edu> One of our pathologists wants to know if anyone is running IHC apoptosis markers (caspase 3 or similar) on cattle. Thanks, Karen Sverlow CAHFS, UC Davis From G.Spoelstra <@t> murdoch.edu.au Fri Nov 14 02:28:46 2014 From: G.Spoelstra <@t> murdoch.edu.au (Gerard Spoelstra) Date: Fri Nov 14 02:29:27 2014 Subject: [Histonet] RE: Processed blocks before decaling In-Reply-To: <778DD853CF606049A37FC2059C8BA07A9EFB8EB725@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A9EFB8EB725@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: Hi I have found that blocks that were insufficiently decalcified, responded best when taken back to formalin left in buffered formalin for 24 hrs and then decalcified. If you take them back to water then decalcify them, decalcification will cause more damage to the tissue then if they spend some more time in formalin. You can also put them through the clean cycle to take them back to water. Gerard Spoelstra Veterinary Histology Murdoch University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori.Disher@HCAhealthcare.com Sent: Friday, 14 November 2014 1:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processed blocks before decaling Hello, We had several tissue cassettes put on the processor before they were decaled. Can I just melt the blocks, run thru xylene, alcohol & water. Put in decal and re-process? Thanks in advance for your expert advice! Lori Disher Lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV <@t> mail.amc.edu Fri Nov 14 07:50:17 2014 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Fri Nov 14 07:50:23 2014 Subject: [Histonet] Space Issues Message-ID: Hi everyone, I was wondering what other labs were doing as far as tracking in very small lab areas (such as grossing rooms, microtomy stations, embedding stations). Are you using laptops, touchscreens, regular PC's, etc. and what type of mounting have you found that works, etc. . We are implementing a new computer system which will include specimen tracking and have VERY limited space so any suggestions anyone might have would be greatly appreciated. Thank you, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From galinadeyneko <@t> yahoo.com Fri Nov 14 09:20:43 2014 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Fri Nov 14 09:23:48 2014 Subject: [Histonet] Re: Sections contamination after Lac Z staining Message-ID: <1415978443.8847.YahooMailNeo@web160204.mail.bf1.yahoo.com> Dear Colleagues I search your advices regarding Lac Z staining on frozen OCT embedded brain. After staining in Xgal staining solution overnight at 37C all sections covered by glassy small particles. The sections themselves are not contaminated, i checked them with H&E staining and also checked under microscope before placing in the beta gal staining solution. The blue Lac Z staining is good and intense. Short method description:fix brain in freshly prepared Glutaraldehyde-Formaline fixative 2 hours, after Sucrose embed in OCT. Section thickness 7 microns. For staining I use Millipore kit. Of cause I warm the stain base solution at 37C before to dilute melted beta Gal stock and the staining solution is clean and transparent, no precipitation. After staining fix sections in 10% formalin for 15 minutes and counterstain in Nuclear Fast red for 40 seconds. I have try several modifications: no rinse the sections in the DI water, rinse the sections in DI water to get rid of OCT, after staining, and after counterstaining, nothing help. I am even not able to catch the moment when appears this contamination. maybe filter the beta gal staining solution before placing slides? please share your suggestions, or detailed protocols. Thank you in advance Galina Deyneko Novartis, Cambridge, MA 617-871-7613 w ________________________________ From Timothy.Morken <@t> ucsf.edu Fri Nov 14 09:30:07 2014 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Fri Nov 14 09:30:16 2014 Subject: [Histonet] RE: Space Issues In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367B692B@ex07.net.ucsf.edu> Vicki for the same reasons we went with all-in-one touchscreen computers (monitor/computer in one unit) that are mounted via articulated arms to a track on the wall, so nothing on the bench. The track allows infinite positioning possibilities. We use the Cognitive Cxi1300 label printer at each microtome, which has a smaller footprint than a zebra, and mini-keyboards that include a touch pad. Some like a mouse, however so we got wireless mice for those people. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Friday, November 14, 2014 5:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Space Issues Hi everyone, I was wondering what other labs were doing as far as tracking in very small lab areas (such as grossing rooms, microtomy stations, embedding stations). Are you using laptops, touchscreens, regular PC's, etc. and what type of mounting have you found that works, etc. . We are implementing a new computer system which will include specimen tracking and have VERY limited space so any suggestions anyone might have would be greatly appreciated. Thank you, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Fri Nov 14 09:35:19 2014 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Fri Nov 14 09:36:03 2014 Subject: [Histonet] clarificaiton RE: Space Issues Message-ID: <761E2B5697F795489C8710BCC72141FF367B6959@ex07.net.ucsf.edu> To clarify, we got the mini keyboards to use with the touch screens because it is still easier to log in with a keyboard than an on-screen keyboard, and Copath is still not fully useable via touchscreen - the text and buttons are too small. The next version (v2013) will have better touch screen capability. However, even with that caveat once logged in there is little interaction with the computer beyond scanning a block or slide. Oh, we have the Honeywell 1900 scanners. Highly programmable for your needs. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, November 14, 2014 7:30 AM To: 'Gauch, Vicki'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Space Issues Vicki for the same reasons we went with all-in-one touchscreen computers (monitor/computer in one unit) that are mounted via articulated arms to a track on the wall, so nothing on the bench. The track allows infinite positioning possibilities. We use the Cognitive Cxi1300 label printer at each microtome, which has a smaller footprint than a zebra, and mini-keyboards that include a touch pad. Some like a mouse, however so we got wireless mice for those people. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gauch, Vicki Sent: Friday, November 14, 2014 5:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Space Issues Hi everyone, I was wondering what other labs were doing as far as tracking in very small lab areas (such as grossing rooms, microtomy stations, embedding stations). Are you using laptops, touchscreens, regular PC's, etc. and what type of mounting have you found that works, etc. . We are implementing a new computer system which will include specimen tracking and have VERY limited space so any suggestions anyone might have would be greatly appreciated. Thank you, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From W.E.J.Hoekert <@t> olvg.nl Fri Nov 14 09:58:41 2014 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Fri Nov 14 09:58:47 2014 Subject: [Histonet] RE: Endogenous Tau (normal Tau) In-Reply-To: <2A1220D9-2517-4808-A431-107798C6D28E@nsalabs.com> References: <2A1220D9-2517-4808-A431-107798C6D28E@nsalabs.com> Message-ID: Hi Claudia, We use Dako anti-Human Tau (rabbit polyclonal) at a concentration of 1:8000. We do not use a pretreatment (HIER or protease). Staining is done on a Ventana stainer. Good luck, Willem ________________________________________ Van: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] namens Segovia, Claudia [csegovia@nsalabs.com] Verzonden: donderdag 6 november 2014 19:23 Aan: Onderwerp: [Histonet] Endogenous Tau (normal Tau) Hi there, I am writing a poster on different Tau epitopes in human brain free floating sections. I have been unable to get any normal Tau and I wonder what could be the problem. Does anybody know what could be? Also, does anyone have a protocol that can share with me? Thank you! Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csegovia@nsalabs.com STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csegovia@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From Allison.Scott <@t> harrishealth.org Fri Nov 14 10:08:19 2014 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Fri Nov 14 10:08:29 2014 Subject: [Histonet] Performance Improvement Indicators Message-ID: Happy Friday to all in histoland. What type of indicators are you guys tracking for PI reports(tat, specimen quality). I am looking to track more that FStat and specimen quality. Any help with this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From rjbuesa <@t> yahoo.com Fri Nov 14 10:34:14 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 14 10:34:36 2014 Subject: [Histonet] Performance Improvement Indicators In-Reply-To: References: Message-ID: <923020295.507285.1415982854858.JavaMail.yahoo@jws100199.mail.ne1.yahoo.com> Allison:I used several indicators that were appropriate for my lab. Example:1- having the RUSH specimens ready for the pathologists when they arrived was of extreme importance, so I kept a list showing the?time when the first and the last rush went to the office and improving on both was one indicator2- similarly, when the last case of the day was ready for the pathologists was another3- other were individual indicators such as: blocks cut/day (to calculate per hour) 4- total amount of work in each aspect (routine, FS,?HC, IHC, FISH)I shared all this information with the staff and it was posted in a vissible place.In my last job we?(a commercial reference lab) I was contracted to solve an efficiency problem because there was a cases lag "two weeks worth", meaning that for a new block to be cut/stained there was a 2 weeks delay.So my indicator was "how many block were left the previous day in the freezer uncut". With a designated plan in 9 days our "lag" became "0 blocks" and from that moment on the uncut blocks averaged 22 only because on?the week ends we had?less personnel.My point is that you should look at ALL your tasks and they TAT and include ALL.You cannot pick-and-choose some tasks because all are important and you should try to cover all.Start with those more important for your pathologists because remember that, although we in histology have to have the patient in mind always, our direct CLIENTS are the pathologists. We have to give them the best slide possible to they can take care of the correct diagnosis for the patients.Just a thought! Ren? J. On Friday, November 14, 2014 11:08 AM, "Scott, Allison D" wrote: Happy Friday to all in histoland.? What type of indicators are you guys tracking for PI reports(tat, specimen quality).? I am looking to track more that FStat? and specimen quality.? Any help with this will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LBUSTAMANTE <@t> cvm.tamu.edu Fri Nov 14 12:05:16 2014 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Fri Nov 14 12:05:21 2014 Subject: [Histonet] TSH new worshop submission Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC901505050D0@CVMMB02.cvm.tamu.edu> Could you please let us know WHEN and HOW to submit a workshop for the next TSH? Thank you very much for the information. Lin Bustamante and Rayen Gonzalez Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From Lyndsey.Buechel <@t> covance.com Fri Nov 14 15:31:01 2014 From: Lyndsey.Buechel <@t> covance.com (Buechel, Lyndsey) Date: Fri Nov 14 15:31:42 2014 Subject: [Histonet] Histology Professionals Needed Message-ID: Covance, a global contract research organization is looking for a number of histology professionals to join our team in Madison, WI. Relocation assistance is available. We offer competitive wages and excellent benefits, including 21 days of paid time off. Research Assistant - at least 3 years of histology and/or necropsy experience Sr. Study Technician - at least 2 years of histology and/or necropsy experience. Please visit www.covancecareers.com to apply to the corresponding 5 digit number or reach out to me directly with any questions. Lyndsey Buechel Recruiter Recruitment and Talent Advisors Covance Inc. Minneapolis, MN Tel: +1 218 297 4125 E-mail: lyndsey.buechel@covance.com Apply online at: www.covancecareers.com ________________________________ Confidentiality Notice: In accordance with Covance's Data Classification Policy, this email, including attachment(s), is classified as Confidential or Highly Confidential. This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or dissemination of the content of this e-mail is strictly prohibited. If you have received this e-mail transmission in error or this email is not intended for you, please delete or destroy all copies of this message in your possession and inform the sender. Thank you. From equineshowmom01 <@t> yahoo.com Sat Nov 15 08:48:37 2014 From: equineshowmom01 <@t> yahoo.com (Sheree H) Date: Sat Nov 15 08:51:36 2014 Subject: [Histonet] Acetone fixation Message-ID: <1416062917.75585.YahooMailIosMobile@web162901.mail.bf1.yahoo.com> Have questions about using acetone at the grossing counter. Does anyone have problems with it being carried over into the formalin on the processor? We rinse, open cassette to let it dry completely and even change the cassette. I've had issues with my processor and want to make sure it's not coming from the acetone. [1] Sent from Yahoo Mail for iPhone References 1. https://overview.mail.yahoo.com/?.src=iOS From rsrichmond <@t> gmail.com Sat Nov 15 14:34:19 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Nov 15 14:34:22 2014 Subject: [Histonet] Re: Acetone fixation Message-ID: Sheree H (where?) asks: >>Have questions about using acetone at the grossing counter. Does anyone have problems with it being carried over into the formalin on the processor? We rinse, open cassette to let it dry completely and even change the cassette. I've had issues with my processor and want to make sure it's not coming from the acetone.<< Acetone doesn't belong in the grossing area - too much of a fire and explosion hazard. If your pathologists are using it to fix marking ink onto specimens, they can use 3% acetic acid (half-strength white vinegar). I don't use anything at all - if you blot the specimen dry before you put the ink on, it'll stay put. Bob Richmond Samurai Pathologist Maryville TN From tony.henwood <@t> health.nsw.gov.au Sun Nov 16 17:00:36 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Nov 16 17:00:51 2014 Subject: [Histonet] RE: Processed blocks before decaling In-Reply-To: References: <778DD853CF606049A37FC2059C8BA07A9EFB8EB725@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53C99B2@xmdb04.nch.kids> I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gerard Spoelstra Sent: Friday, 14 November 2014 7:29 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Processed blocks before decaling Hi I have found that blocks that were insufficiently decalcified, responded best when taken back to formalin left in buffered formalin for 24 hrs and then decalcified. If you take them back to water then decalcify them, decalcification will cause more damage to the tissue then if they spend some more time in formalin. You can also put them through the clean cycle to take them back to water. Gerard Spoelstra Veterinary Histology Murdoch University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori.Disher@HCAhealthcare.com Sent: Friday, 14 November 2014 1:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processed blocks before decaling Hello, We had several tissue cassettes put on the processor before they were decaled. Can I just melt the blocks, run thru xylene, alcohol & water. Put in decal and re-process? Thanks in advance for your expert advice! Lori Disher Lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From twheelock <@t> mclean.harvard.edu Mon Nov 17 08:07:14 2014 From: twheelock <@t> mclean.harvard.edu (Wheelock, Timothy R.) Date: Mon Nov 17 08:08:44 2014 Subject: [Histonet] HistoPro 150 Embedding Center Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9773C2741@PHSX10MB11.partners.org> Hi Everyone: Has anyone had experience with the Rushabh HistoPro 150 embedding center? Could you share your thoughts and opinions about this machine? Thank you, Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Lynn.Burton <@t> Illinois.gov Mon Nov 17 08:35:15 2014 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Mon Nov 17 08:35:26 2014 Subject: [Histonet] Re: Acetone fixation In-Reply-To: References: Message-ID: We use acetone to denature fatty tumors without any problems. They sit in it for at least half an hour. I don't rinse them. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Saturday, November 15, 2014 2:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Acetone fixation Sheree H (where?) asks: >>Have questions about using acetone at the grossing counter. Does >>anyone have problems with it being carried over into the formalin on the processor? We rinse, open cassette to let it dry completely and even change the cassette. I've had issues with my processor and want to make sure it's not coming from the acetone.<< Acetone doesn't belong in the grossing area - too much of a fire and explosion hazard. If your pathologists are using it to fix marking ink onto specimens, they can use 3% acetic acid (half-strength white vinegar). I don't use anything at all - if you blot the specimen dry before you put the ink on, it'll stay put. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From murphyv <@t> karmanos.org Mon Nov 17 09:00:06 2014 From: murphyv <@t> karmanos.org (Murphy, Valerie) Date: Mon Nov 17 09:00:16 2014 Subject: [Histonet] Tissue Processors Message-ID: We are looking to purchase a new tissue processor for our tissue core. The workload is quite light. Can anyone recommend a small, reliable processor ? Thank you, Valerie Ratliff BS HT(ASCP) Wayne State University Detroit, Michigan ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. From sbaldwin <@t> mhhcc.org Mon Nov 17 09:48:54 2014 From: sbaldwin <@t> mhhcc.org (Baldwin, Kathy) Date: Mon Nov 17 09:49:04 2014 Subject: [Histonet] FW: Job Opportunity In-Reply-To: <9e3d7cb10c43496c8e91f4e30af8a1bf@exch02.mhhcc.org> References: <9e3d7cb10c43496c8e91f4e30af8a1bf@exch02.mhhcc.org> Message-ID: <52183325994d4853814c4ffa845d05fc@exch02.mhhcc.org> Hi Histonetters!! Histology Tech position: Full time Monday thru Friday. Jasper, Indiana 47546 Jasper is a regional center in southwestern Indiana, noted for its German Catholic ancestral roots Jasper has often been called the "Wood Capital of the World", boasting a large number of furniture companies, including Kimball International and Masterbrand Cabinets. Jasper is also home to the Southern Indiana Education Service Center (SIEC), Jasper Engines & Transmissions (largest remanufacturer in the market), and to a satellite campus of Vincennes University. JOB SUMMARY: Responsible for embedding, sectioning and cover slipping paraffin blocks and staining tissues to provide the pathologist with accurately prepared specimens for review and diagnosis. The employee works with specimens from patients of all ages. The employee must reflect mission statement and philosophy of Memorial Hospital and Health Care Center in daily work habits and contacts. QUALIFICATIONS: Education: High school diploma or equivalent is required. Associate degree preferred. HT (ASCP) certification is preferred. Three years' experience as histology assist is acceptable. Training: Six months on the job training required. Experience: Previous histology experience is preferred. New graduates of histology school will be considered. S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbaldwin@mhhcc.org "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" From brannon <@t> alliedsearchpartners.com Mon Nov 17 10:37:56 2014 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Mon Nov 17 10:38:11 2014 Subject: [Histonet] Histology Supervisor job opening near Buffalo, NY Message-ID: We are currently recruiting for a client in the Buffalo, NY area for a Histology Supervisor with a valid NY lab license and 6 years of experience including experience as a leader. Interested candidates please forward an updated resume to brannon@alliedsearchpartners.com for review. To view a complete list of Allied Search Partners current openings go to: http://www.jobs.net/jobs/alliedsearchpartners/en-us/ -- Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 From abadesuyi <@t> nrh-ok.com Mon Nov 17 12:50:02 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Mon Nov 17 12:50:13 2014 Subject: [Histonet] ER, PR, HER2 CARGES Message-ID: <04EE4F75BB5FB246ADB68D69B746044398FF8AD770@MAIL.nrhnt.nrh-ok.com> Hi Guys, Please can you guys tell me the CPT Codes for ER, PR and HER2? Thanks for your anticipated cooperation. Thanks, Banjo ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From WaitT <@t> livemail.uthscsa.edu Mon Nov 17 12:58:50 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Mon Nov 17 12:58:57 2014 Subject: [Histonet] TRAP staining protocol troubleshooting Message-ID: <1416250732752.74848@livemail.uthscsa.edu> Hello histonetter heroes! I have an issue with a TRAP staining protocol that I'm currently using that involves Fast Red Violet LB Salt for the staining of the Osteoclast and then Fast Green as a counterstain. The osteoclast are suppose to stain Red Violet and the background is suppose to stain green. However, I'm not seeing any Red Violet Osteoclast but just really really Green tissue. The bone I was using was cortical bone but surely there would at least 1 Osteoclast in all of sections that I had. Here is the protocol that I'm using: Trap Staining solution Mix: Trap Basic Incubation medium, Fast Red Violet LB Salt, and Naphthol AS-MX Phophaget substrate mix Procedure: 1. Place TRAP staining solution mix in staining dish and pre-warm to 37 degree celsius in waterbath 2. Deparafinnize slides and rehydrate through graded ethanols to distilled water. 3. Place slides in pre-warmed TRAP staining solution mix and incubate at 37 celsius for 30 mins or until control is developed. 4. Rinse in distilled wtaer 5. Counterstain with 0.02% Fast Green for 30 seconds and rinse quickly in distilled water. 6. Dehydrate quickly thorugh graded alcohols, 5 seconds each, clear in Xylene and mount. The problem is that the main staining with the Fast Red is not very substantial.....at least not as much as it should be. When I take the slide from the water bath, the tissue section is only slightly red...and may even look more orangish yellow....which is a long ways away from red violet. Then when I counterstain with Fast Green, I feel that the Fast Green stain is so prominent that it just superimposes the Fast Red stain. For anyone that has experience with TRAP staining or Fast Red Violet staining then that would be awesome to hear your input! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry From Carolyn.Barnes2 <@t> va.gov Mon Nov 17 13:05:28 2014 From: Carolyn.Barnes2 <@t> va.gov (Barnes, Carolyn K. RICVAMC) Date: Mon Nov 17 13:06:28 2014 Subject: [Histonet] Bone Marrow processing using Immunocal Message-ID: I am looking for a Bone Marrow procedure that uses Immunocal as the decalcifier. I am particularly interested in how long they fix in Immunocal and if heat is used in any way . Our lab just started using this product because we are under the impression that it gave better ISH results. Thank-you all so much for any assistance. I really appreciate everyone's help. Carolyn K. Barnes Histology Supervisor McGuire VA Medical Center Richmond, VA 23249 Ph: 804-675-5000, x2158 From WaitT <@t> livemail.uthscsa.edu Mon Nov 17 13:07:42 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Mon Nov 17 13:07:46 2014 Subject: [Histonet] Decalcified Bone Clearing time Message-ID: <1416251263334.63026@livemail.uthscsa.edu> Hey everyone! I'm having some issues about clearing the bone tissues after they get done dehydrating from decalcification. I know that over clearing the bone tissues in Xylene for too long can cause them to become very brittle and I'm afraid that that was what was happening. The bone blocks are not very big....their weights are listed as 0.222g, 0.100g, and 0.052g. The biggest bone block is about 4mmx4mmx4mm so it's not very big I would say. I cleared with 50% Ethanol/50% Xylene for 1 hour, and then I changed it with 100% Xylene for another hour. The bone blocks become clear as expected but they begin to get a yellowish-tan tinge to them. I'm not the most experienced but from what I've studied, I don't think that 2 hours in Xylene is overkill by any means so maybe it's something that I'm doing subconsciously.....if anyone has any suggestions are ideas please let me know! :) Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry From dr <@t> personifysearch.com Mon Nov 17 13:35:34 2014 From: dr <@t> personifysearch.com (Danielle Robinson) Date: Mon Nov 17 13:35:39 2014 Subject: [Histonet] New Position Available - Field Support Scientist: Molecular Message-ID: Hi All, This is an exclusively retained opportunity with a world leader in cancer diagnostics. Our client has acquired a company that specializes in molecular FISH probes, and they have recently opened an opportunity on the West Coast for a Field Support Scientist. The ideal candidate will be currently working in a Cytogenetics Laboratory and will have extensive FISH experience. This is a customer facing role with a lot of growth opportunity. The ideal location is anywhere on the West Coast The company offers a strong package including a base salary, competitive commission structure, company car, gas card, cell phone, laptop computer, and full benefits. If you or anyone you know many be interested in learning more about this position, please contact me directly at dr@personifysearch.com Thank you so much, Danielle Robinson Talent Management Executive 5020 Weston Parkway, Ste 315 Cary, NC 27513 US Toll Free: +1 (800) 875-6188 ext. 140 International Access Phone: +1.919.473.3762 http://www.personifysearch.com/ http://www.linkedin.com/pub/danielle-robinson/23/5aa/70a From clemenma <@t> evms.edu Mon Nov 17 14:03:56 2014 From: clemenma <@t> evms.edu (Clements, Mary Ann) Date: Mon Nov 17 14:04:06 2014 Subject: [Histonet] Laser Capture Microdissection Instrument Message-ID: <7C3ADBB9F8E8E442B69BDE04519091DB0D2FD008@Trillya.evms.net> We are in the process of purchasing a LCM system, I'm interested in your opinions of Leica and Arcturus. Regards, Mary Ann Clements, B.S. Biorepository Manager Eastern Virginia Medical School Leroy T. Canoles Jr. Cancer Research Center Lewis Hall, Room 3018 700 West Olney Road Norfolk, VA 23501 email: clemenma@evms.edu phone: (757) 446-7910 fax: (757) 446- 7059 From melissa <@t> alliedsearchpartners.com Mon Nov 17 14:31:03 2014 From: melissa <@t> alliedsearchpartners.com (Melissa Owens (Phelan)) Date: Mon Nov 17 14:31:20 2014 Subject: [Histonet] MOHS Technician Job in Arizona Message-ID: Hello, I have a MOHS Technician Job in Arizona for Full Time/Direct Hire. Please message me for more details and the job description. Thank you, Melissa Owens (Phelan) Allied Search Partners From pruegghm <@t> hotmail.com Mon Nov 17 14:40:20 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Mon Nov 17 14:40:29 2014 Subject: [Histonet] TRAP staining protocol troubleshooting In-Reply-To: References: <1416250732752.74848@livemail.uthscsa.edu>, Message-ID: Actually trap will work if you pre incubate it in 0.2M Tris buffer Ph9 (use sodium hydroxide to pH) for 1 hour at 37dc then proceed with the trap stain without rinsing. Check the stain in 20 min as it develops quickly after this. This protocol was given to me by the late great Hermina and it works really well even on formic acid decaled FFPE bone in my hands. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From: pruegghm@hotmail.com To: waitt@livemail.uthscsa.edu Subject: RE: [Histonet] TRAP staining protocol troubleshooting Date: Mon, 17 Nov 2014 13:22:24 -0700 Trap dose not work on decalcified bone unless you have decaled with EDTA in my experience. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net > From: WaitT@livemail.uthscsa.edu > To: histonet@lists.utsouthwestern.edu > Date: Mon, 17 Nov 2014 18:58:50 +0000 > Subject: [Histonet] TRAP staining protocol troubleshooting > > Hello histonetter heroes! I have an issue with a TRAP staining protocol that I'm currently using that involves Fast Red Violet LB Salt for the staining of the Osteoclast and then Fast Green as a counterstain. The osteoclast are suppose to stain Red Violet and the background is suppose to stain green. However, I'm not seeing any Red Violet Osteoclast but just really really Green tissue. The bone I was using was cortical bone but surely there would at least 1 Osteoclast in all of sections that I had. Here is the protocol that I'm using: > > > Trap Staining solution Mix: Trap Basic Incubation medium, Fast Red Violet LB Salt, and Naphthol AS-MX Phophaget substrate mix > > > Procedure: > > 1. Place TRAP staining solution mix in staining dish and pre-warm to 37 degree celsius in waterbath > > 2. Deparafinnize slides and rehydrate through graded ethanols to distilled water. > > 3. Place slides in pre-warmed TRAP staining solution mix and incubate at 37 celsius for 30 mins or until control is developed. > > 4. Rinse in distilled wtaer > > 5. Counterstain with 0.02% Fast Green for 30 seconds and rinse quickly in distilled water. > > 6. Dehydrate quickly thorugh graded alcohols, 5 seconds each, clear in Xylene and mount. > > > The problem is that the main staining with the Fast Red is not very substantial.....at least not as much as it should be. When I take the slide from the water bath, the tissue section is only slightly red...and may even look more orangish yellow....which is a long ways away from red violet. Then when I counterstain with Fast Green, I feel that the Fast Green stain is so prominent that it just superimposes the Fast Red stain. For anyone that has experience with TRAP staining or Fast Red Violet staining then that would be awesome to hear your input! > > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio > Class of 2017 MD Candidate > Abilene Christian University Class of 2013 Graduate > B.S. Biochemistry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Mon Nov 17 14:47:51 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Mon Nov 17 14:47:55 2014 Subject: [Histonet] Decalcified Bone Clearing time In-Reply-To: <1416251263334.63026@livemail.uthscsa.edu> References: <1416251263334.63026@livemail.uthscsa.edu> Message-ID: Make sure that you fix well in formalin before you decal (what are you using?), then I rinse off the decal solution (I use formic acid) and put them back into formalin before putting on the tissue processor for a longer than usual schedule and especially give them longer time to infiltrate in paraffin, at least 3 changes for 2 hours each after dehydration and clearing in xylene. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net > From: WaitT@livemail.uthscsa.edu > To: histonet@lists.utsouthwestern.edu > Date: Mon, 17 Nov 2014 19:07:42 +0000 > Subject: [Histonet] Decalcified Bone Clearing time > > Hey everyone! I'm having some issues about clearing the bone tissues after they get done dehydrating from decalcification. I know that over clearing the bone tissues in Xylene for too long can cause them to become very brittle and I'm afraid that that was what was happening. The bone blocks are not very big....their weights are listed as 0.222g, 0.100g, and 0.052g. The biggest bone block is about 4mmx4mmx4mm so it's not very big I would say. I cleared with 50% Ethanol/50% Xylene for 1 hour, and then I changed it with 100% Xylene for another hour. The bone blocks become clear as expected but they begin to get a yellowish-tan tinge to them. I'm not the most experienced but from what I've studied, I don't think that 2 hours in Xylene is overkill by any means so maybe it's something that I'm doing subconsciously.....if anyone has any suggestions are ideas please let me know! :) > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio > Class of 2017 MD Candidate > Abilene Christian University Class of 2013 Graduate > B.S. Biochemistry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Mon Nov 17 14:54:00 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Mon Nov 17 14:54:05 2014 Subject: [Histonet] Bone Marrow processing using Immunocal In-Reply-To: References: Message-ID: I use a platform shaker and slosh the sample around while it is in decal solution at RT, just make sure it is well fixed before you decal, Immunocal is 5% formic acid decal solution it is not a fixative and it is a bit slower than some of the rapid decal reagents out there but is much better for IHC. When I was in HemePath we would fix for about 6 hrs before decaling on the shaker for 2-4hrs depending on the size (thickness) of the bone bx. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net > Date: Mon, 17 Nov 2014 14:05:28 -0500 > From: Carolyn.Barnes2@va.gov > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Bone Marrow processing using Immunocal > > I am looking for a Bone Marrow procedure that uses Immunocal as the > decalcifier. I am particularly interested in how long they fix in > Immunocal and if heat is used in any way . Our lab just started using > this product because we are under the impression that it gave better ISH > results. > > Thank-you all so much for any assistance. I really appreciate everyone's > help. > > > > Carolyn K. Barnes > > Histology Supervisor > > McGuire VA Medical Center > > Richmond, VA 23249 > > Ph: 804-675-5000, x2158 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WaitT <@t> livemail.uthscsa.edu Mon Nov 17 15:30:00 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Mon Nov 17 15:30:11 2014 Subject: [Histonet] TRAP staining protocol troubleshooting In-Reply-To: References: <1416250732752.74848@livemail.uthscsa.edu>, , Message-ID: That's interesting because I buffered it to 4.7-5.0......that's actually a huge difference.....does that have to do with he Fast Red approach? Sent from my iPhone On Nov 17, 2014, at 2:40 PM, "Patsy Ruegg" > wrote: Actually trap will work if you pre incubate it in 0.2M Tris buffer Ph9 (use sodium hydroxide to pH) for 1 hour at 37dc then proceed with the trap stain without rinsing. Check the stain in 20 min as it develops quickly after this. This protocol was given to me by the late great Hermina and it works really well even on formic acid decaled FFPE bone in my hands. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net ________________________________ From: pruegghm@hotmail.com To: waitt@livemail.uthscsa.edu Subject: RE: [Histonet] TRAP staining protocol troubleshooting Date: Mon, 17 Nov 2014 13:22:24 -0700 Trap dose not work on decalcified bone unless you have decaled with EDTA in my experience. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net > From: WaitT@livemail.uthscsa.edu > To: histonet@lists.utsouthwestern.edu > Date: Mon, 17 Nov 2014 18:58:50 +0000 > Subject: [Histonet] TRAP staining protocol troubleshooting > > Hello histonetter heroes! I have an issue with a TRAP staining protocol that I'm currently using that involves Fast Red Violet LB Salt for the staining of the Osteoclast and then Fast Green as a counterstain. The osteoclast are suppose to stain Red Violet and the background is suppose to stain green. However, I'm not seeing any Red Violet Osteoclast but just really really Green tissue. The bone I was using was cortical bone but surely there would at least 1 Osteoclast in all of sections that I had. Here is the protocol that I'm using: > > > Trap Staining solution Mix: Trap Basic Incubation medium, Fast Red Violet LB Salt, and Naphthol AS-MX Phophaget substrate mix > > > Procedure: > > 1. Place TRAP staining solution mix in staining dish and pre-warm to 37 degree celsius in waterbath > > 2. Deparafinnize slides and rehydrate through graded ethanols to distilled water. > > 3. Place slides in pre-warmed TRAP staining solution mix and incubate at 37 celsius for 30 mins or until control is developed. > > 4. Rinse in distilled wtaer > > 5. Counterstain with 0.02% Fast Green for 30 seconds and rinse quickly in distilled water. > > 6. Dehydrate quickly thorugh graded alcohols, 5 seconds each, clear in Xylene and mount. > > > The problem is that the main staining with the Fast Red is not very substantial.....at least not as much as it should be. When I take the slide from the water bath, the tissue section is only slightly red...and may even look more orangish yellow....which is a long ways away from red violet. Then when I counterstain with Fast Green, I feel that the Fast Green stain is so prominent that it just superimposes the Fast Red stain. For anyone that has experience with TRAP staining or Fast Red Violet staining then that would be awesome to hear your input! > > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio > Class of 2017 MD Candidate > Abilene Christian University Class of 2013 Graduate > B.S. Biochemistry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Mon Nov 17 15:56:51 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Nov 17 15:56:59 2014 Subject: [Histonet] PgR Negative Cases for 2014 Message-ID: Did anyone else see an increase of PgR Negative cases early in 2014? Seems like there was an increase in the % of cases that were ER + but PgR negative. We saw an increase in cases particularly from Jan to Jun30th. Any thoughts? Thanks in advance, Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com From cindy38017 <@t> yahoo.com Mon Nov 17 17:43:11 2014 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Mon Nov 17 17:43:16 2014 Subject: [Histonet] competency checklist Message-ID: <1239200107.687425.1416267791273.JavaMail.yahoo@jws10784.mail.gq1.yahoo.com> How are laboratories handling the competency checklist? Do you have one list that is all inclusive or blank forms and fill in with the competency observed? Thanks, Cindy From PAMarcum <@t> uams.edu Tue Nov 18 08:26:19 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Nov 18 08:26:26 2014 Subject: [Histonet] competency checklist In-Reply-To: <1239200107.687425.1416267791273.JavaMail.yahoo@jws10784.mail.gq1.yahoo.com> References: <1239200107.687425.1416267791273.JavaMail.yahoo@jws10784.mail.gq1.yahoo.com> Message-ID: <531b960e8ed9428f8a2e801af53e75fc@MAIL13M2N1.ad.uams.edu> We actually have two. We have the simplified one CAP wants and the comprehensive one we need for our histologists. They are very different. CAP just wants to know basics in a short form as far as our administration was concerned. We developed our own internal to measure how well people are doing and assess where they need to improve or where we can improve the lab. CAP does not see the more comprehensive one and when they did it was more than they needed. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cindy dewar Sent: Monday, November 17, 2014 5:43 PM To: Histonet Subject: [Histonet] competency checklist How are laboratories handling the competency checklist? Do you have one list that is all inclusive or blank forms and fill in with the competency observed? Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Dennis.Hahn <@t> cookchildrens.org Tue Nov 18 10:18:18 2014 From: Dennis.Hahn <@t> cookchildrens.org (Dennis Hahn) Date: Tue Nov 18 10:18:31 2014 Subject: [Histonet] Rubber "mats" for pinning specimens Message-ID: Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 From BDeBrosse-Serra <@t> isisph.com Tue Nov 18 10:20:42 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Nov 18 10:20:48 2014 Subject: [Histonet] RE: Rubber "mats" for pinning specimens In-Reply-To: References: Message-ID: Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, November 18, 2014 8:18 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Rubber "mats" for pinning specimens Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Nov 18 10:24:36 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Nov 18 10:24:47 2014 Subject: [Histonet] RE: Rubber "mats" for pinning specimens In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F12E8BB@evcspmbx2.ads.northwestern.edu> We make up paraffin trays, either in a cafeteria tray (sssh....) or in the lid of a slide box if it's for something small. Works like a charm and supplies are at hand. Besides that, paraffin floats in a formalin tank as well. You could have custom cut trays and still use your paraffin..... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, November 18, 2014 10:21 AM To: Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber "mats" for pinning specimens Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, November 18, 2014 8:18 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Rubber "mats" for pinning specimens Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Nov 18 11:33:00 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Nov 18 11:33:11 2014 Subject: [Histonet] RE: Rubber "mats" for pinning specimens In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF2F12E8BB@evcspmbx2.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF2F12E8BB@evcspmbx2.ads.northwestern.edu> Message-ID: <25A4DE08332B19499904459F00AAACB719E8D747EB@EVS1.archildrens.org> We use Styrofoam and break it to the right size. Get them out of shipping boxes. Free! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Tuesday, November 18, 2014 10:25 AM To: Bea DeBrosse-Serra; Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber "mats" for pinning specimens We make up paraffin trays, either in a cafeteria tray (sssh....) or in the lid of a slide box if it's for something small. Works like a charm and supplies are at hand. Besides that, paraffin floats in a formalin tank as well. You could have custom cut trays and still use your paraffin..... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, November 18, 2014 10:21 AM To: Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber "mats" for pinning specimens Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, November 18, 2014 8:18 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Rubber "mats" for pinning specimens Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From HornHV <@t> archildrens.org Tue Nov 18 11:49:49 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Nov 18 11:50:08 2014 Subject: [Histonet] tissue tek 5 embedding center Message-ID: <25A4DE08332B19499904459F00AAACB719E8D747ED@EVS1.archildrens.org> Can anyone tell me if the Tissue Tek 5 has a work light? Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From kdwyatt <@t> vet.k-state.edu Tue Nov 18 12:53:32 2014 From: kdwyatt <@t> vet.k-state.edu (Kristina Wyatt) Date: Tue Nov 18 12:53:41 2014 Subject: [Histonet] Microtome ergonimic evaluation Message-ID: <658a77fb9cf848b39c8dd607d1bb382d@SN2PR0501MB1039.namprd05.prod.outlook.com> Has anyone ever had an ergonomic evaluation specific to their microtome station? If so, could you please give me the contact information of the evaluator? Thanks, Kristina Wyatt, M.A. Supervisor: Histopathology/Immunohistochemistry L216 Mosier Hall Kansas State Veterinary Diagnostic Laboratory 785-532-4464 kdwyatt@vet.k-state.edu From ludlow <@t> HHSC.CA Tue Nov 18 13:13:46 2014 From: ludlow <@t> HHSC.CA (Ludlow Patricia) Date: Tue Nov 18 13:13:56 2014 Subject: [Histonet] Flames at embedding centers Message-ID: Is anyone out there still using flames at the embedding centers for their forceps? If not, what are you using instead? Pat Ludlow Technical Specialist/Supervisor Histology HRLMP 905-522-1155 X35954 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From jaylundgren <@t> gmail.com Tue Nov 18 13:42:28 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Nov 18 13:42:31 2014 Subject: [Histonet] Flames at embedding centers In-Reply-To: References: Message-ID: Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) > > > From lblazek <@t> digestivespecialists.com Tue Nov 18 15:03:47 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Nov 18 15:03:55 2014 Subject: [Histonet] Flames at embedding centers In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> Grinning and remembering the "good old days". What's more fun is the look of horror on the faces of the young ones when they hear it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Nov 18 15:14:22 2014 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Nov 18 15:14:27 2014 Subject: [Histonet] Flames at embedding centers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> Message-ID: <267176184.2227533.1416345262439.JavaMail.yahoo@jws106100.mail.bf1.yahoo.com> Like! & ditto!?Paula Pierce,BS,HTL(ASCP)HT?President?Excalibur Pathology, Inc.?5830 N Blue Lake Dr.?Norman, OK 73069?405-759-3953 PH?405-759-7513 FAX www.excaliburpathology.com From: "Blazek, Linda" To: Jay Lundgren ; Ludlow Patricia Cc: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 18, 2014 3:03 PM Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the "good old days".? What's more fun is the look of horror on the faces of the young ones when they hear it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers ? ? Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning.? I didn't have any hair on my knuckles for years.? Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone?? Good times. ? ? Now we have to use forceps warmers and change forceps between specimens.? If you get 3 or 4 pair of forceps, one will always be hot enough to use.? Also, there are embedding centers with heated forceps, which I love . ? ? Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination.? Cotton applicator swabs work great for this.? And always keep a towel or gauze handy to wipe the tips of the forceps between specimens.? Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. ? ? ? ? ? ? ? ? ? ? ? Sincerely, ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren, M.S., HTL (ASCP) > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Tue Nov 18 15:21:05 2014 From: b427297 <@t> aol.com (William J. O'Connor III) Date: Tue Nov 18 15:21:11 2014 Subject: [Histonet] Flames at embedding centers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> Message-ID: <8D1D18CAF2078D4-938-42E50@webmail-vm146.sysops.aol.com> The open flames were great because they incinerated any residual tissue fragments, like from EMCs. Hardy ever had cross contamination back in those days. Lab fires, yes - I remember one in the late 1960's at St. Elizabeth Hospital in Chicago. (Just heard about it, wasn't there at the time) Alcohol lamp ignited xylene in the lab on the 8th floor. Bad story. I can see the reason to not have open flames anymore. We used loop incinerators from the Bac-T lab for awhile, too, instead of open flames. Jackie O' -----Original Message----- From: Blazek, Linda To: Jay Lundgren ; Ludlow Patricia Cc: histonet Sent: Tue, Nov 18, 2014 3:04 pm Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the "good old days". What's more fun is the look of orror on the faces of the young ones when they hear it! -----Original Message----- rom: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] n Behalf Of Jay Lundgren ent: Tuesday, November 18, 2014 2:42 PM o: Ludlow Patricia c: histonet@lists.utsouthwestern.edu ubject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen urners first thing in the morning. I didn't have any hair on my knuckles for ears. Embedding with a Coke on the cold plate, and a smoke in the ashtray next o you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. f you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, here are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to revent cross contamination. Cotton applicator swabs work great for this. And lways keep a towel or gauze handy to wipe the tips of the forceps between pecimens. Forceps warmers unfortunately don't incinerate any stray tissue like Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) > ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Nov 18 15:22:42 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Nov 18 15:22:53 2014 Subject: [Histonet] Flames at embedding centers In-Reply-To: <267176184.2227533.1416345262439.JavaMail.yahoo@jws106100.mail.bf1.yahoo.com> References: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> <267176184.2227533.1416345262439.JavaMail.yahoo@jws106100.mail.bf1.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB719E8D747FB@EVS1.archildrens.org> Yes, the good old days!....And, we survived! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children?s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, November 18, 2014 3:14 PM To: Blazek, Linda; Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Like! & ditto!?Paula Pierce,BS,HTL(ASCP)HT?President?Excalibur Pathology, Inc.?5830 N Blue Lake Dr.?Norman, OK 73069?405-759-3953 PH?405-759-7513 FAX www.excaliburpathology.com From: "Blazek, Linda" To: Jay Lundgren ; Ludlow Patricia Cc: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 18, 2014 3:03 PM Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the "good old days".? What's more fun is the look of horror on the faces of the young ones when they hear it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers ? ? Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning.? I didn't have any hair on my knuckles for years.? Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone?? Good times. ? ? Now we have to use forceps warmers and change forceps between specimens.? If you get 3 or 4 pair of forceps, one will always be hot enough to use.? Also, there are embedding centers with heated forceps, which I love . ? ? Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination.? Cotton applicator swabs work great for this.? And always keep a towel or gauze handy to wipe the tips of the forceps between specimens.? Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. ? ? ? ? ? ? ? ? ? ? ? Sincerely, ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren, M.S., HTL (ASCP) > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From lblazek <@t> digestivespecialists.com Tue Nov 18 15:23:36 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Nov 18 15:23:43 2014 Subject: [Histonet] Flames at embedding centers In-Reply-To: <25A4DE08332B19499904459F00AAACB719E8D747FB@EVS1.archildrens.org> References: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> <267176184.2227533.1416345262439.JavaMail.yahoo@jws106100.mail.bf1.yahoo.com> <25A4DE08332B19499904459F00AAACB719E8D747FB@EVS1.archildrens.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E39172A4F07D3@IBMB7Exchange.digestivespecialists.com> We're well preserved! -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Tuesday, November 18, 2014 4:23 PM To: 'Paula Pierce'; Blazek, Linda; Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flames at embedding centers Yes, the good old days!....And, we survived! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children?s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, November 18, 2014 3:14 PM To: Blazek, Linda; Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Like! & ditto!?Paula Pierce,BS,HTL(ASCP)HT?President?Excalibur Pathology, Inc.?5830 N Blue Lake Dr.?Norman, OK 73069?405-759-3953 PH?405-759-7513 FAX www.excaliburpathology.com From: "Blazek, Linda" To: Jay Lundgren ; Ludlow Patricia Cc: "histonet@lists.utsouthwestern.edu" Sent: Tuesday, November 18, 2014 3:03 PM Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the "good old days".? What's more fun is the look of horror on the faces of the young ones when they hear it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers ? ? Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning.? I didn't have any hair on my knuckles for years.? Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone?? Good times. ? ? Now we have to use forceps warmers and change forceps between specimens.? If you get 3 or 4 pair of forceps, one will always be hot enough to use.? Also, there are embedding centers with heated forceps, which I love . ? ? Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination.? Cotton applicator swabs work great for this.? And always keep a towel or gauze handy to wipe the tips of the forceps between specimens.? Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. ? ? ? ? ? ? ? ? ? ? ? Sincerely, ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren, M.S., HTL (ASCP) > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From PAMarcum <@t> uams.edu Tue Nov 18 15:22:15 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Nov 18 16:22:41 2014 Subject: [Histonet] Flames at embedding centers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> Message-ID: <68070b72f77649979b91b51f11589d3c@MAIL13M2N1.ad.uams.edu> We have days when one of the histologist who has been here for years and I (going in 50years) start talking about the good days and everyone here is under 40. They look shocked and then disbelief and then they think we are kidding. They are so sure we have always known which chemicals were dangerous or killers and no one could possibly ever have been so careless. WOW! I like to show them old equipment and ask how they think it might work in a lecture on tissue processing. They have no clue about an open processor in small room with no ventilation and we worked in it with our open stains lines and flames on the counter. Now it does sound scary! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, November 18, 2014 3:04 PM To: Jay Lundgren; Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flames at embedding centers Grinning and remembering the "good old days". What's more fun is the look of horror on the faces of the young ones when they hear it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, November 18, 2014 2:42 PM To: Ludlow Patricia Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flames at embedding centers Ahh, the good old days of walking into the lab and lighting all the Bunsen burners first thing in the morning. I didn't have any hair on my knuckles for years. Embedding with a Coke on the cold plate, and a smoke in the ashtray next to you, anyone? Good times. Now we have to use forceps warmers and change forceps between specimens. If you get 3 or 4 pair of forceps, one will always be hot enough to use. Also, there are embedding centers with heated forceps, which I love . Just remember to clean out the wells of the forceps warmers every day to prevent cross contamination. Cotton applicator swabs work great for this. And always keep a towel or gauze handy to wipe the tips of the forceps between specimens. Forceps warmers unfortunately don't incinerate any stray tissue like a Bunsen burner did. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From bakevictoria <@t> gmail.com Tue Nov 18 18:17:13 2014 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Nov 18 18:17:17 2014 Subject: [Histonet] Flames at embedding centers In-Reply-To: <68070b72f77649979b91b51f11589d3c@MAIL13M2N1.ad.uams.edu> References: <5A2BD13465E061429D6455C8D6B40E39172A4F07C6@IBMB7Exchange.digestivespecialists.com> <68070b72f77649979b91b51f11589d3c@MAIL13M2N1.ad.uams.edu> Message-ID: My very first Histo job was with Metpath in Hackensack NJ. We had Bunsen burners right next to a xylene bath for dirty molds. One day a tech accidentally had a xylene Bunsen burner collision and started a fire. We had another tech who was quick with head and feet who was able to extinguish it - but there was damage. As to smoking in the lab I do remember. Between the fumes and the smoke I found breathing a little difficult. But yes those were the "good 'ol days". Vikki On Nov 18, 2014 5:22 PM, "Marcum, Pamela A" wrote: > We have days when one of the histologist who has been here for years and I > (going in 50years) start talking about the good days and everyone here is > under 40. They look shocked and then disbelief and then they think we are > kidding. They are so sure we have always known which chemicals were > dangerous or killers and no one could possibly ever have been so careless. > WOW! I like to show them old equipment and ask how they think it might > work in a lecture on tissue processing. They have no clue about an open > processor in small room with no ventilation and we worked in it with our > open stains lines and flames on the counter. Now it does sound scary! > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda > Sent: Tuesday, November 18, 2014 3:04 PM > To: Jay Lundgren; Ludlow Patricia > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Flames at embedding centers > > Grinning and remembering the "good old days". What's more fun is the look > of horror on the faces of the young ones when they hear it! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren > Sent: Tuesday, November 18, 2014 2:42 PM > To: Ludlow Patricia > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Flames at embedding centers > > Ahh, the good old days of walking into the lab and lighting all the > Bunsen burners first thing in the morning. I didn't have any hair on my > knuckles for years. Embedding with a Coke on the cold plate, and a smoke > in the ashtray next to you, anyone? Good times. > > Now we have to use forceps warmers and change forceps between > specimens. If you get 3 or 4 pair of forceps, one will always be hot > enough to use. Also, there are embedding centers with heated forceps, > which I love . > Just remember to clean out the wells of the forceps warmers every day > to prevent cross contamination. Cotton applicator swabs work great for > this. And always keep a towel or gauze handy to wipe the tips of the > forceps between specimens. Forceps warmers unfortunately don't incinerate > any stray tissue like a Bunsen burner did. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Yves.Heremans <@t> vub.ac.be Tue Nov 18 20:08:11 2014 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Tue Nov 18 20:08:19 2014 Subject: [Histonet] double staining alkaline phosphatase Message-ID: <5ECDC01F-7513-44BC-B28C-A8BBC4D54D60@vub.ac.be> Dear Histonetters, Is it technically possible to perform a double staining using alkaline phosphatase in both stainings ? I was thinking to perform a seqeuntial staining and develop the staining for the first protein and detect with a blue AP substrate and then perform staining for the second protein and detect with a red AP substrate. However, after the first staining, I would need to be able to completely block AP-activity or circumvent cross-reaction in some way. Yves From melissa <@t> alliedsearchpartners.com Wed Nov 19 06:51:43 2014 From: melissa <@t> alliedsearchpartners.com (Melissa Owens (Phelan)) Date: Wed Nov 19 06:51:58 2014 Subject: [Histonet] Direct/Perm Hire for Traveling MOHS Tech in Southern CA Message-ID: Good Morning, Just an update that I have a Direct/Permanent hire opportunity for an experienced Traveling MOHS Technician in Southern California. Please message me if you are interested. Melissa Owens Allied Search Partners From algranth <@t> email.arizona.edu Wed Nov 19 08:37:35 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Nov 19 08:38:00 2014 Subject: [Histonet] RE: Rubber "mats" for pinning specimens In-Reply-To: <25A4DE08332B19499904459F00AAACB719E8D747EB@EVS1.archildrens.org> References: <62C639732D3F274DACED033EBDF6ADAF2F12E8BB@evcspmbx2.ads.northwestern.edu>, <25A4DE08332B19499904459F00AAACB719E8D747EB@EVS1.archildrens.org> Message-ID: We used the paraffin trays too and also made them to use as cutting boards. Didn't dull the blades either. Cafeteria trays worked well for making them! Our cafeteria surely didn't miss the one we swiped for this purpose! Andi Happily retired now! ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Horn, Hazel V [HornHV@archildrens.org] Sent: Tuesday, November 18, 2014 10:33 AM To: 'Bernice Frederick'; Bea DeBrosse-Serra; Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber "mats" for pinning specimens We use Styrofoam and break it to the right size. Get them out of shipping boxes. Free! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Tuesday, November 18, 2014 10:25 AM To: Bea DeBrosse-Serra; Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber "mats" for pinning specimens We make up paraffin trays, either in a cafeteria tray (sssh....) or in the lid of a slide box if it's for something small. Works like a charm and supplies are at hand. Besides that, paraffin floats in a formalin tank as well. You could have custom cut trays and still use your paraffin..... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, November 18, 2014 10:21 AM To: Dennis Hahn; 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Rubber "mats" for pinning specimens Have you tried to pin on large cork sheets? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, November 18, 2014 8:18 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Rubber "mats" for pinning specimens Can anyone tell me if there is a company that sells the rubber mats for pinning open larger specimens, such as colons? We are currently using cooled paraffin as our pinning surface. I have found one company that sells small pans with the rubber already inside of it, but I'm looking for something we can cut to fit our current containers, no small specimen pans needed. The pathologists have also stated that they do NOT want cork. Thanks again, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Wed Nov 19 12:16:01 2014 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Nov 19 12:16:28 2014 Subject: [Histonet] Re: Rubber mats for pinning specimens Message-ID: <24B7B291CC88D04AB663958E77A1F59D1D0384@ex09.net.ucsf.edu> We don't have a need to pin where I currently work but at past labs I have used flat pieces of foam, like the lids from cooler boxes that used to ship reagents that are temperature sensitive. You can cut them to fit whatever container you are going to use to fix the specimen. Place a few paper towels on the foam before you put the specimen down to pin - it will allow the formalin to get under the tissue. Foam can be a bit crumbly, like cork, but since you are repurposing something that would have gone in the trash you can always throw them out after the specimen is fixed. Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From rsrichmond <@t> gmail.com Wed Nov 19 12:36:24 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Nov 19 12:36:28 2014 Subject: [Histonet] Re: Flames at embedding centers Message-ID: Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN From Milton.Gomez <@t> nyumc.org Wed Nov 19 12:42:06 2014 From: Milton.Gomez <@t> nyumc.org (Gomez, Milton) Date: Wed Nov 19 12:42:19 2014 Subject: [Histonet] Re: Flames at embedding centers In-Reply-To: References: Message-ID: <2B0D905D71C9AA4B859B99F404A6C4A11116D019@MSGWCDCPMB27.nyumc.org> It would be interesting to create an album of memoirs to share with our younger generations. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichmond@gmail.com] Sent: Wednesday, November 19, 2014 1:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Nov 19 12:50:28 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Nov 19 12:50:33 2014 Subject: [Histonet] Re: Flames at embedding centers In-Reply-To: <2B0D905D71C9AA4B859B99F404A6C4A11116D019@MSGWCDCPMB27.nyumc.org> References: <2B0D905D71C9AA4B859B99F404A6C4A11116D019@MSGWCDCPMB27.nyumc.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E39172A4F09DC@IBMB7Exchange.digestivespecialists.com> "It would be interesting to create an album of memoirs to share with our younger generations." Calling Sally, calling Sally..... this is a good one for you to start! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gomez, Milton Sent: Wednesday, November 19, 2014 1:42 PM To: Bob Richmond; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers It would be interesting to create an album of memoirs to share with our younger generations. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Bob Richmond [rsrichmond@gmail.com] Sent: Wednesday, November 19, 2014 1:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Wed Nov 19 12:58:29 2014 From: JWatson <@t> gnf.org (James Watson) Date: Wed Nov 19 12:58:36 2014 Subject: [Histonet] Re: Flames at embedding centers In-Reply-To: References: Message-ID: How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Nov 19 13:03:35 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Nov 19 13:03:50 2014 Subject: [Histonet] Re: Flames at embedding centers In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B7811FE64FD8@EMBX-CHAM2.cdc.gov> the good old days.................................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Nov 19 13:08:13 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Nov 19 13:08:20 2014 Subject: [Histonet] Re: Flames at embedding centers In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FE64FD8@EMBX-CHAM2.cdc.gov> References: <3B2CD438E1628A41BD687E98B963B7811FE64FD8@EMBX-CHAM2.cdc.gov> Message-ID: <5A2BD13465E061429D6455C8D6B40E39172A4F09ED@IBMB7Exchange.digestivespecialists.com> Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers the good old days.................................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Nov 19 13:09:43 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Nov 19 13:10:19 2014 Subject: [Histonet] Re: Flames at embedding centers In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39172A4F09ED@IBMB7Exchange.digestivespecialists.com> References: <3B2CD438E1628A41BD687E98B963B7811FE64FD8@EMBX-CHAM2.cdc.gov> <5A2BD13465E061429D6455C8D6B40E39172A4F09ED@IBMB7Exchange.digestivespecialists.com> Message-ID: <3B2CD438E1628A41BD687E98B963B7811FE64FF9@EMBX-CHAM2.cdc.gov> Okay, since we are confessing......... When I was a student in histology school, we did the potluck thing IN THE LAB! I mean, ALL the food was laid out on a back counter................................ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, November 19, 2014 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers the good old days.................................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Wed Nov 19 13:34:41 2014 From: Jessica.Vacca <@t> HCAhealthcare.com (Jessica.Vacca@HCAhealthcare.com) Date: Wed Nov 19 13:37:47 2014 Subject: [Histonet] Snomed Message-ID: How many of your pathologists, LIS or others are using Snomed? Jessica Vacca HCA Epic Anatomic Pathology Application Lead Clinical Services Group 2545 Park Plaza Bldg III Nashville, TN From Joyce.Weems <@t> emoryhealthcare.org Wed Nov 19 13:45:02 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Nov 19 13:45:23 2014 Subject: [Histonet] RE: Snomed In-Reply-To: References: Message-ID: Not here - haven't in years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Wednesday, November 19, 2014 2:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Snomed How many of your pathologists, LIS or others are using Snomed? Jessica Vacca HCA Epic Anatomic Pathology Application Lead Clinical Services Group 2545 Park Plaza Bldg III Nashville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From mturner <@t> CSILaboratories.com Wed Nov 19 13:53:14 2014 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Wed Nov 19 13:53:33 2014 Subject: [Histonet] Re: Flames at embedding centers In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FE64FF9@EMBX-CHAM2.cdc.gov> References: <3B2CD438E1628A41BD687E98B963B7811FE64FD8@EMBX-CHAM2.cdc.gov> <5A2BD13465E061429D6455C8D6B40E39172A4F09ED@IBMB7Exchange.digestivespecialists.com> <3B2CD438E1628A41BD687E98B963B7811FE64FF9@EMBX-CHAM2.cdc.gov> Message-ID: <643626B74DE2814D8537057F40E1A10B1537D173@CSI-MX-NODEA.CSI-LABS.local> We had a special "clean" counter we used for pizza on a regular basis. I worked with a pathologist who refused to wear gloves and would gross colons bare-handed. Guy is still alive and kicking at 85! In the very old days, we used carbon tetrachloride to dehydrate in the open tissue processor (Technicon). Not going to say anything at all about disposal.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:10 PM To: Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Okay, since we are confessing......... When I was a student in histology school, we did the potluck thing IN THE LAB! I mean, ALL the food was laid out on a back counter................................ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, November 19, 2014 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers the good old days.................................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Nov 19 14:45:54 2014 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Nov 19 14:45:59 2014 Subject: [Histonet] Re: Flames at embedding centers In-Reply-To: <643626B74DE2814D8537057F40E1A10B1537D173@CSI-MX-NODEA.CSI-LABS.local> References: <3B2CD438E1628A41BD687E98B963B7811FE64FD8@EMBX-CHAM2.cdc.gov> <5A2BD13465E061429D6455C8D6B40E39172A4F09ED@IBMB7Exchange.digestivespecialists.com> <3B2CD438E1628A41BD687E98B963B7811FE64FF9@EMBX-CHAM2.cdc.gov>, <643626B74DE2814D8537057F40E1A10B1537D173@CSI-MX-NODEA.CSI-LABS.local> Message-ID: Old Histotechs never die. They're just well fixed... :). ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Mark Turner [mturner@CSILaboratories.com] Sent: Wednesday, November 19, 2014 1:53 PM To: Sanders, Jeanine (CDC/OID/NCEZID); Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers We had a special "clean" counter we used for pizza on a regular basis. I worked with a pathologist who refused to wear gloves and would gross colons bare-handed. Guy is still alive and kicking at 85! In the very old days, we used carbon tetrachloride to dehydrate in the open tissue processor (Technicon). Not going to say anything at all about disposal.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:10 PM To: Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Okay, since we are confessing......... When I was a student in histology school, we did the potluck thing IN THE LAB! I mean, ALL the food was laid out on a back counter................................ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, November 19, 2014 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers the good old days.................................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Thu Nov 20 04:38:29 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Nov 20 04:38:41 2014 Subject: [Histonet] RE: Snomed In-Reply-To: References: Message-ID: We haven't used snomed codes for many years. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica.Vacca@HCAhealthcare.com Sent: Wednesday, November 19, 2014 2:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Snomed How many of your pathologists, LIS or others are using Snomed? Jessica Vacca HCA Epic Anatomic Pathology Application Lead Clinical Services Group 2545 Park Plaza Bldg III Nashville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From b-frederick <@t> northwestern.edu Thu Nov 20 07:16:14 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Nov 20 07:16:23 2014 Subject: [Histonet] Re: Flames at embedding centers In-Reply-To: References: <3B2CD438E1628A41BD687E98B963B7811FE64FD8@EMBX-CHAM2.cdc.gov> <5A2BD13465E061429D6455C8D6B40E39172A4F09ED@IBMB7Exchange.digestivespecialists.com> <3B2CD438E1628A41BD687E98B963B7811FE64FF9@EMBX-CHAM2.cdc.gov>, <643626B74DE2814D8537057F40E1A10B1537D173@CSI-MX-NODEA.CSI-LABS.local> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F130CD3@evcspmbx2.ads.northwestern.edu> Darn right! We'll live forever at this rate of "fixation"....... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, November 19, 2014 2:46 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Old Histotechs never die. They're just well fixed... :). ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Mark Turner [mturner@CSILaboratories.com] Sent: Wednesday, November 19, 2014 1:53 PM To: Sanders, Jeanine (CDC/OID/NCEZID); Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers We had a special "clean" counter we used for pizza on a regular basis. I worked with a pathologist who refused to wear gloves and would gross colons bare-handed. Guy is still alive and kicking at 85! In the very old days, we used carbon tetrachloride to dehydrate in the open tissue processor (Technicon). Not going to say anything at all about disposal.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:10 PM To: Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Okay, since we are confessing......... When I was a student in histology school, we did the potluck thing IN THE LAB! I mean, ALL the food was laid out on a back counter................................ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, November 19, 2014 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers the good old days.................................... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From galinadeyneko <@t> yahoo.com Thu Nov 20 09:39:35 2014 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Thu Nov 20 09:43:01 2014 Subject: [Histonet] Re: mouse carotid body Message-ID: <1731614667.3144585.1416497975698.JavaMail.yahoo@jws10685.mail.bf1.yahoo.com> ?Dear Colleagues.?Please share your expertise how to reveal the carotid body in mouse carotid artery. ?I received from researcher the piece of mouse carotid artery with bifurcation on internal and external carotid branches. What is the best way for embedding: flat parallel to the mold bottom or on transverse axis, that is perpendicular to the mold bottom.? I think I should collect many serial sections and maybe in couple of them I will be able to find carotid body. Am I right? Please advise better and rational way to do this.Thank you?Galina DeynekoNovartis, Cambridge, MA?617-871-7613 w ?? From lcolbert <@t> pathmdlabs.com Thu Nov 20 10:45:40 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Thu Nov 20 10:45:49 2014 Subject: [Histonet] HPV ISH Message-ID: <12ECD7346266D74691EC2BFC75285E45381392CC@BFL323E10.pathmdlabs.local> What do others do for HPV ISH? Do you do the stain yourself in-house, or do you send it out? I know there used to be a stainer specifically for ISH - does this still exist and who is the vendor? I heard there is some kind of conflict between vendors and the FDA?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From Dorothy.L.Webb <@t> HealthPartners.Com Thu Nov 20 12:53:42 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Nov 20 12:53:47 2014 Subject: [Histonet] open histology position Message-ID: <96eac457ecbd4f3babdc305323ca7915@HPEXCH05.HealthPartners.int> Temporary Histotechnologist Position in St. Paul, MN Interested in making some extra holiday cash? Regions Hospital is looking for an experienced histotechnologist to work a temporary, full time night shift through January 31, 2015. Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com. Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and "Best Care, Best Experience", has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will perform tests and procedures in our histology laboratory; perform functions associated with obtaining, receiving and processing of surgical pathology specimens in patients of all ages, to maintain laboratory equipment and supplies, and to perform related duties as assigned. Qualifications: Histotechnician board certification, ASCP or equivalent certification preferred. Two (2) years of Histology work experience within the last five (5) years may substitute for the educational qualifications. Education: Completion of an approved histology program and clinical histology internship or completion of an accredited two year laboratory technician training program in a vocational school or junior college. How to Apply: Apply online at www.regionshospital.com> Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. Dorothy Webb, HT (ASCP) 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From egray <@t> hsc.wvu.edu Thu Nov 20 13:30:31 2014 From: egray <@t> hsc.wvu.edu (Gray, Ed) Date: Thu Nov 20 13:30:42 2014 Subject: [Histonet] Re: Flames at embedding centers Message-ID: Ah, such an appropriate time of year for this thread. Once a year we gave our trash autoclave a good cleaning and pressure cooked turkeys for our Thanksgiving party. Ed Gray | Clinical Application Coordinator | Phone: 304-293-2945 | Fax: 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sforeman <@t> labpath.com Thu Nov 20 13:56:41 2014 From: sforeman <@t> labpath.com (Susan Foreman) Date: Thu Nov 20 14:02:14 2014 Subject: [Histonet] CD 34 and CD117 Message-ID: <006701d004fc$1a659900$4f30cb00$@com> Who are your favorite vendors for CD34 (blasts & basophils) and CD117 (immature granulocytes) in bone marrow tissues. Currently utilizing the Ventana Benchmark Ultra platform. Interested in finding a different vendor that will make our pathologist happier. Our current antibody is working, but he's just not happy. Many Thanks for All your Input, Susan From mdmurphy <@t> alaska.edu Thu Nov 20 15:12:44 2014 From: mdmurphy <@t> alaska.edu (Molly Murphy) Date: Thu Nov 20 15:12:52 2014 Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Message-ID: Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 From ttruscot <@t> vetmed.wsu.edu Thu Nov 20 15:23:47 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Nov 20 15:24:06 2014 Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate In-Reply-To: References: Message-ID: <9EF5279EBDFE6E4FB6605E8F183A0027D154A38E@CVM76.vetmed.wsu.edu> Hi Molly, You might just want to fix in buffered 10% formalin and then rinse specimens well in water ( maybe 20 minutes) before using in class and then put back in formalin. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Thursday, November 20, 2014 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Nov 20 15:37:01 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Nov 20 15:37:15 2014 Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate In-Reply-To: <9EF5279EBDFE6E4FB6605E8F183A0027D154A38E@CVM76.vetmed.wsu.edu> References: <9EF5279EBDFE6E4FB6605E8F183A0027D154A38E@CVM76.vetmed.wsu.edu> Message-ID: How about formalin substitute from Anatech - Propar I think it is.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Thursday, November 20, 2014 4:24 PM To: Molly Murphy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi Molly, You might just want to fix in buffered 10% formalin and then rinse specimens well in water ( maybe 20 minutes) before using in class and then put back in formalin. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Thursday, November 20, 2014 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From epollock <@t> ucalgary.ca Thu Nov 20 17:22:16 2014 From: epollock <@t> ucalgary.ca (Betty Pollock) Date: Thu Nov 20 17:22:30 2014 Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate In-Reply-To: References: Message-ID: We are a veterinary pathology teaching lab and we used Klotz with chloral hydrate to preserve our gross teaching specimens. We switched to a Klotz recipe that does not use chloral hydrate because the chloral hydrate is hard to source and very expensive. It is maybe not quite as good as the Klotz with chloral hydrate but seems to work OK. Here is the recipe. Sodium chloride 208 g Sodium bicarbonate 375 g Sodium sulfate 458 g Formaldehyde, 37% 667 ml Water To make up to 50 L Regards, Betty Pollock Manager, Operations DSU Faculty of Veterinary Medicine University of Calgary Calgary, AB, Canada Tel: 403-220-2806 FAX: 403-239-6984 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Thursday, November 20, 2014 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Nov 20 18:48:36 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Nov 20 18:48:58 2014 Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53CAA57@xmdb04.nch.kids> Well if the specimens are well fixed in formalin, then bring back the colour with alcohol and you can the store them in a non-formalin solution such as dilute honey or high salt solution: Henwood, A., (2002) "Color preservation in pathology museum specimens" Biotechnic & Histochem 77(4):230. ?zkan, N., Salva, E., Cakalagaoglu, F., & T?z?ner, B. (2012). Honey as a substitute for formalin?. Biotechnic & Histochemistry, 87(2), 148-153. Al-Maaini R, Bryant P (2006) The effectiveness of honey as a substitute for formalin in the histological fixation of tissue. J. Histotechnol. 29: 173?176. Oliveira, F. S. (2014). Assessing the effectiveness of 30% sodium chloride aqueous solution for the preservation of fixed anatomical specimens: a 5?year follow?up study. J. Anat. (2014) 225, pp118-121 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Friday, 21 November 2014 8:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From mikael.niku <@t> helsinki.fi Fri Nov 21 04:23:21 2014 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Nov 21 04:23:32 2014 Subject: [Histonet] Intestinal mucus -- Carnoy or what? In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF394FE140@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF394FE140@CHIEX005.CHI.catholichealth.net> Message-ID: <546F1299.3030809@helsinki.fi> Hello! What is the best way to fix intestinal samples in order to preserve the mucus (and the embedded bacteria)? I was recommended Carnoy's, but are there any alternatives... perhaps without chloroform? With best regards, Mikael From joost.bruijntjes <@t> tno.triskelion.nl Fri Nov 21 04:33:28 2014 From: joost.bruijntjes <@t> tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Fri Nov 21 04:33:37 2014 Subject: [Histonet] (no subject) Message-ID: Hi all Is anyone of you using a an alternative for the DAKO-ARK kit. In my opinion it is good, but rather expensive. Best regards, Joost Bruijntjes From Dr.Harst <@t> gmx.net Fri Nov 21 05:09:15 2014 From: Dr.Harst <@t> gmx.net (Dr.Harst@gmx.net) Date: Fri Nov 21 05:09:20 2014 Subject: [Histonet] embedding of insects in historesin (Technovit 7100) Message-ID: Hallo, are there any experiences in embedding of insects in historesin (GMA or Glycolmethacrylat (Technovit 7100))? I have tried Technovit after fixing in Formaldehyd with poor results. The specimen tends to fail out of the block. There is no connection between the cuticula and the resin. Therefore it is not possible, to stretch the ribbon on the surface of water. And I believe there is some interaction between the chitin and the resin which makes trouble. The chitin is brittle after embedding in resin. I have tried different times in the embedding process, but without good results. Embedding single organs for example ovaries is without any difficulties . Obviously chitin makes the problem. Perhaps helps another fixing or some other pretreatment? Thanks for a little help ! Jürgen From dr.harst <@t> gmx.net Fri Nov 21 07:00:00 2014 From: dr.harst <@t> gmx.net (=?utf-8?Q?J=C3=BCrgen_harst?=) Date: Fri Nov 21 07:00:01 2014 Subject: [Histonet] sectioning bee's heads and mites Message-ID: <3EBBF343-8C0A-449A-B1E7-0C54D616545A@gmx.net> From mikael.niku <@t> helsinki.fi Fri Nov 21 08:16:00 2014 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Nov 21 08:16:14 2014 Subject: [Histonet] Intestinal mucus -- Carnoy or what? (and: dry methanol??) In-Reply-To: <546F1299.3030809@helsinki.fi> References: <852F7D2C14FB464D80E182B15DB138AF394FE140@CHIEX005.CHI.catholichealth.net> <546F1299.3030809@helsinki.fi> Message-ID: <546F4920.8060600@helsinki.fi> Oh, sorry, I forgot one additional question regarding Carnoy's: in the protocols I found, Carnoy's is prepared using DRY methanol. Sorry for my ignorance, but does this mean that methanol has to be specifically treated to remove any hygroscopic etc water? Note that this is specifically intended for preserving intestinal mucus, which I guess might be sensitive to water-containing fixatives (although I presume there can't be more water in ordinary methanol than introduced by the tissue sample itself). With best regards, Mikael On 21.11.2014 12:23, Mikael Niku wrote: > Hello! > > What is the best way to fix intestinal samples in order to preserve > the mucus (and the embedded bacteria)? > I was recommended Carnoy's, but are there any alternatives... perhaps > without chloroform? > > With best regards, > Mikael > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Fri Nov 21 09:41:54 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Fri Nov 21 09:41:58 2014 Subject: [Histonet] embedding of insects in historesin (Technovit 7100) In-Reply-To: References: Message-ID: First of all, this sounds like a Damien Laudier question. Damien, I hope you can help this guy. Secondly, do you have to use plastic embedding? I was successful with paraffin embedding. I was able to section various small insects and also bees. It takes some patience and soaking in mollifex or glycerin water but the chitin became less brittle and able to section without ripping out the internal organs. Andi G. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Dr.Harst@gmx.net [Dr.Harst@gmx.net] Sent: Friday, November 21, 2014 4:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding of insects in historesin (Technovit 7100) Hallo, are there any experiences in embedding of insects in historesin (GMA or Glycolmethacrylat (Technovit 7100))? I have tried Technovit after fixing in Formaldehyd with poor results. The specimen tends to fail out of the block. There is no connection between the cuticula and the resin. Therefore it is not possible, to stretch the ribbon on the surface of water. And I believe there is some interaction between the chitin and the resin which makes trouble. The chitin is brittle after embedding in resin. I have tried different times in the embedding process, but without good results. Embedding single organs for example ovaries is without any difficulties . Obviously chitin makes the problem. Perhaps helps another fixing or some other pretreatment? Thanks for a little help ! J?rgen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Fri Nov 21 09:57:05 2014 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Nov 21 09:57:10 2014 Subject: [Histonet] Thermo Scientific Richard-Allan Mark-it dyes Message-ID: I already contacted them about this, but I thought that all of you might be faster: Would anyone happen to know if these dyes, if used to mark mouse bone/tissue around said bone at necropsy, would survive decalcification? We're trying to facilitate orientation of OA mouse knees during embedding Thanks and have a good weekend, Kathleen Roberts Principal Lab Technician Histopathology Lab Office of Translational Sciences Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From Dr.Harst <@t> gmx.net Fri Nov 21 11:55:41 2014 From: Dr.Harst <@t> gmx.net (Dr.Harst@gmx.net) Date: Fri Nov 21 11:55:48 2014 Subject: [Histonet] embedding of insects in historesin (Technovit 7100) Message-ID: Hallo, Andi G. Thanks for your reply. Normally I use paraffine for embedding insects after dehydration and using N Butylalkohol with sucess. In my opinion it is absolutly necessary to avoid any rests of water and to cut cool. But this technique is limited to sections of 3-4 µ and heating is unavoidable. I would be very grateful for a reply of Damien Laudier or of someone else, who could help me in this matter. Jürgen From LBUSTAMANTE <@t> cvm.tamu.edu Fri Nov 21 12:21:54 2014 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Fri Nov 21 12:22:06 2014 Subject: [Histonet] Use of Polar Heat Pad inside Microwave Processor Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC90150517685@CVMMB02.cvm.tamu.edu> I need a good, reliable company to buy from. This pad is not cheap. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From jaylundgren <@t> gmail.com Fri Nov 21 12:30:43 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Nov 21 12:30:47 2014 Subject: [Histonet] Use of Polar Heat Pad inside Microwave Processor In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC90150517685@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC90150517685@CVMMB02.cvm.tamu.edu> Message-ID: http://www.instructables.com/id/Microwave-Heating-Pad/ Like this? On Fri, Nov 21, 2014 at 12:21 PM, Bustamante, Lin wrote: > I need a good, reliable company to buy from. This pad is not cheap. > Thank you. > Lin. > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Laboratory Supervisor > College Of Veterinary Medicine > Texas A&M University > College Station, Texas 77843-4458 > Phone: (979) 845-3177 > Fax: (979) 458-3499 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BergerR1 <@t> email.chop.edu Fri Nov 21 12:49:56 2014 From: BergerR1 <@t> email.chop.edu (Berger, Rebecca) Date: Fri Nov 21 12:50:05 2014 Subject: [Histonet] Fast Green Message-ID: <5A590EB108038B4FA01F8CDB39C6541B05614DB1@EXCMBXPW7.chop.edu> Hey there, does anyone have a good method to de-stain Fast green? The slides need to be re-stained with H&E. Thanks. Rebecca Berger, HT(ASCP)CM Research Technician Division of Orthopedic Surgery The Children's Hospital of Philadelphia Research Institute 3615 Civic Center Boulevard, ARC904 (Lab) Philadelphia, PA 19104 Phone: 267-425-2076 Email: bergerr1@email.chop.edu From sheryl.stephenson <@t> ag.state.nj.us Fri Nov 21 12:59:37 2014 From: sheryl.stephenson <@t> ag.state.nj.us (Stephenson, Sheryl) Date: Fri Nov 21 12:59:41 2014 Subject: [Histonet] lab math: ihc dilution Message-ID: To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl From Donna.Willis <@t> baylorhealth.edu Fri Nov 21 13:02:56 2014 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Fri Nov 21 13:03:08 2014 Subject: [Histonet] Specimen numbering systems Message-ID: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From TanyaAbbott <@t> catholichealth.net Fri Nov 21 13:24:33 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Fri Nov 21 13:24:43 2014 Subject: [Histonet] Recycler Message-ID: <852F7D2C14FB464D80E182B15DB138AF395091F6@CHIEX005.CHI.catholichealth.net> We have a CBG Biotech recycler. We recycle Americlear, xylene substitute. How does everyone dispose of the waste alcohol and waste paraffin? Thanks and have a great weekend! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net From joelleweaver <@t> hotmail.com Fri Nov 21 13:29:37 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Nov 21 13:29:41 2014 Subject: [Histonet] Specimen numbering systems In-Reply-To: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> Message-ID: Numeric for the Specimen and Alpha for the Block. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Donna.Willis@baylorhealth.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 21 Nov 2014 19:02:56 +0000 > Subject: [Histonet] Specimen numbering systems > > Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. > > Thanks, > > Donna Willis, HT/HTL(ASCP) > Anatomic Pathology Manager > > Baylor University Medical Center > 3500 Gaston Ave|Dallas, Texas 75246 > 214-820-2465 office|214-725-6184 mobile > BaylorScottandWhite.com > > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis <@t> baylorhealth.edu Fri Nov 21 13:32:23 2014 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Fri Nov 21 13:32:31 2014 Subject: [Histonet] RE: Specimen numbering systems In-Reply-To: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> Message-ID: <2572B4D63B62E64A8078D8BBE34D407801BDA39C@BHDASVEXML2.bhcs.pvt> Also, How are you specimens identified on your Surgical Requisition, 1,2,3 or A, B, C. Do you use the same identifiers in your LIS and Surgery. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, November 21, 2014 1:03 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AAIFAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=6OI3m7sQR5qu-Enh6Z6bGbmilHJ2Y2lfwXlUfWRJIyk&s=EKDIAnC01YxcqQxvywzQK1ODb6WlOUVUt0GH3b63wKk&e= From liz <@t> premierlab.com Fri Nov 21 13:35:41 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Nov 21 13:35:46 2014 Subject: [Histonet] RE: lab math: ihc dilution In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDC9C@SBS2K8.premierlab.local> Sheryl Here is how I would approach the problem. We would use the basic mathematical equation of the following (in order for this to work you need to keep the units the same) V1 x C1 = V2 x C2 V1 - volume of stock solution needed - this is what you are solving for C1 - concentration of stock solution - you know this its 1.56 mg/ml or 1560 ug/ml (there are 1000 ug in one mg) V2 - volume of final solution needed - I put an arbitrary volume of 10 mls needed C2 - concentration of final solution - you know this its 0.8ug/ml V1 x 1560 ug/ml = 10ml x 0.8 ug/ml --- see how I made sure the units were the same I changed the mg/ml to ug/ml V1 = .0005 mls and then I would need 9.9995 mls of diluent to make up my 0.8ug/ml concentration, you can change this to be expressed in microliters by multiplying each number by 1000. So in uls - V1 = 0.5 uls and 9999.5 uls from there you can determine the titer which is your total volume divided by the volume of concentrated antibody 10/.0005 That would be a 1:20,000 dilution For a dilution such as this I would make up a stock solution of either 1:100 or 1:1000 and then dilute from there. Now its time for the rest of you to check my math, did I do this correctly????? I went over it a few times, but you never know.............. Have a GREAT Weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Friday, November 21, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab math: ihc dilution To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Fri Nov 21 13:36:57 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Fri Nov 21 13:37:02 2014 Subject: [Histonet] Specimen numbering systems In-Reply-To: References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> Message-ID: Same here. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Children's Hospital, Los Angeles -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver Sent: Friday, November 21, 2014 11:30 AM To: Willis, Donna G.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Specimen numbering systems Numeric for the Specimen and Alpha for the Block. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Donna.Willis@baylorhealth.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 21 Nov 2014 19:02:56 +0000 > Subject: [Histonet] Specimen numbering systems > > Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. > > Thanks, > > Donna Willis, HT/HTL(ASCP) > Anatomic Pathology Manager > > Baylor University Medical Center > 3500 Gaston Ave|Dallas, Texas 75246 > 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com > > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From liz <@t> premierlab.com Fri Nov 21 13:37:25 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Nov 21 13:37:32 2014 Subject: [Histonet] RE: Recycler In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF395091F6@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF395091F6@CHIEX005.CHI.catholichealth.net> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDC9D@SBS2K8.premierlab.local> The waste alcohol is disposed of with the rest of our waste chemicals in a 55 gallon drum that picked is up by a licensed waste hauler. The paraffin is also picked up. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Friday, November 21, 2014 12:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycler We have a CBG Biotech recycler. We recycle Americlear, xylene substitute. How does everyone dispose of the waste alcohol and waste paraffin? Thanks and have a great weekend! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Nov 21 13:41:07 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Nov 21 13:41:19 2014 Subject: [Histonet] RE: lab math: ihc dilution In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDC9E@SBS2K8.premierlab.local> Sheryl I will add another bit of information. That final antibody protein concentration is very low. Most of our antibodies (around 60 to 70%) will be in the range of 1 to 10 ug/ml. We do have some antibodies that can have some very low protein concentration though such as neurofilament. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Friday, November 21, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab math: ihc dilution To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kjones <@t> upei.ca Fri Nov 21 13:50:21 2014 From: Kjones <@t> upei.ca (Kathleen Jones) Date: Fri Nov 21 13:50:32 2014 Subject: [Histonet] RE: lab math: ihc dilution In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECDC9C@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECDC9C@SBS2K8.premierlab.local> Message-ID: <546F5F3D0200008B00054C43@oes-grpwise.novell.upei.ca> Hi Liz, Wouldn't you dilute in 999.5ul of diluent, not 9999.5. 1ml=1000ul Otherwise I got the same answer... Kathleen Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)213-2207 >>> Elizabeth Chlipala 21/11/2014 3:35 PM >>> Sheryl Here is how I would approach the problem. We would use the basic mathematical equation of the following (in order for this to work you need to keep the units the same) V1 x C1 = V2 x C2 V1 - volume of stock solution needed - this is what you are solving for C1 - concentration of stock solution - you know this its 1.56 mg/ml or 1560 ug/ml (there are 1000 ug in one mg) V2 - volume of final solution needed - I put an arbitrary volume of 10 mls needed C2 - concentration of final solution - you know this its 0.8ug/ml V1 x 1560 ug/ml = 10ml x 0.8 ug/ml --- see how I made sure the units were the same I changed the mg/ml to ug/ml V1 = .0005 mls and then I would need 9.9995 mls of diluent to make up my 0.8ug/ml concentration, you can change this to be expressed in microliters by multiplying each number by 1000. So in uls - V1 = 0.5 uls and 9999.5 uls from there you can determine the titer which is your total volume divided by the volume of concentrated antibody 10/.0005 That would be a 1:20,000 dilution For a dilution such as this I would make up a stock solution of either 1:100 or 1:1000 and then dilute from there. Now its time for the rest of you to check my math, did I do this correctly????? I went over it a few times, but you never know.............. Have a GREAT Weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Friday, November 21, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab math: ihc dilution To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Nov 21 13:55:44 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Nov 21 13:55:48 2014 Subject: [Histonet] RE: lab math: ihc dilution In-Reply-To: <546F5F3D0200008B00054C43@oes-grpwise.novell.upei.ca> References: <14E2C6176416974295479C64A11CB9AE019C79ECDC9C@SBS2K8.premierlab.local> <546F5F3D0200008B00054C43@oes-grpwise.novell.upei.ca> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDC9F@SBS2K8.premierlab.local> Kathleen I have a final volume of 10 mls not 1 ml in the equation I wrote. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Kathleen Jones [mailto:Kjones@upei.ca] Sent: Friday, November 21, 2014 12:50 PM To: Sheryl Stephenson; histonet@lists.utsouthwestern.edu; Elizabeth Chlipala Subject: [Histonet] RE: lab math: ihc dilution Hi Liz, Wouldn't you dilute in 999.5ul of diluent, not 9999.5. 1ml=1000ul Otherwise I got the same answer... Kathleen Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)213-2207 >>> Elizabeth Chlipala > 21/11/2014 3:35 PM >>> Sheryl Here is how I would approach the problem. We would use the basic mathematical equation of the following (in order for this to work you need to keep the units the same) V1 x C1 = V2 x C2 V1 - volume of stock solution needed - this is what you are solving for C1 - concentration of stock solution - you know this its 1.56 mg/ml or 1560 ug/ml (there are 1000 ug in one mg) V2 - volume of final solution needed - I put an arbitrary volume of 10 mls needed C2 - concentration of final solution - you know this its 0.8ug/ml V1 x 1560 ug/ml = 10ml x 0.8 ug/ml --- see how I made sure the units were the same I changed the mg/ml to ug/ml V1 = .0005 mls and then I would need 9.9995 mls of diluent to make up my 0.8ug/ml concentration, you can change this to be expressed in microliters by multiplying each number by 1000. So in uls - V1 = 0.5 uls and 9999.5 uls from there you can determine the titer which is your total volume divided by the volume of concentrated antibody 10/.0005 That would be a 1:20,000 dilution For a dilution such as this I would make up a stock solution of either 1:100 or 1:1000 and then dilute from there. Now its time for the rest of you to check my math, did I do this correctly????? I went over it a few times, but you never know.............. Have a GREAT Weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Friday, November 21, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab math: ihc dilution To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Fri Nov 21 14:01:10 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Nov 21 14:01:21 2014 Subject: [Histonet] RE: lab math: ihc dilution In-Reply-To: <546F5F3D0200008B00054C43@oes-grpwise.novell.upei.ca> References: <14E2C6176416974295479C64A11CB9AE019C79ECDC9C@SBS2K8.premierlab.local> <546F5F3D0200008B00054C43@oes-grpwise.novell.upei.ca> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A0027D154A5D3@CVM76.vetmed.wsu.edu> When I solve your equation V1 X 1560 ug/ml = 10ml X 0.8 ug/ml I get .005 ml. Therefore the dilution would be closer to 1:2000. I would usually divide the 1560 ug/ml by 0.8 ug/ml and get 1950 therefore 1:1950 would be more accurate . Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Jones Sent: Friday, November 21, 2014 11:50 AM To: Sheryl Stephenson; histonet@lists.utsouthwestern.edu; Elizabeth Chlipala Subject: [Histonet] RE: lab math: ihc dilution Hi Liz, Wouldn't you dilute in 999.5ul of diluent, not 9999.5. 1ml=1000ul Otherwise I got the same answer... Kathleen Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)213-2207 >>> Elizabeth Chlipala 21/11/2014 3:35 PM >>> Sheryl Here is how I would approach the problem. We would use the basic mathematical equation of the following (in order for this to work you need to keep the units the same) V1 x C1 = V2 x C2 V1 - volume of stock solution needed - this is what you are solving for C1 - concentration of stock solution - you know this its 1.56 mg/ml or 1560 ug/ml (there are 1000 ug in one mg) V2 - volume of final solution needed - I put an arbitrary volume of 10 mls needed C2 - concentration of final solution - you know this its 0.8ug/ml V1 x 1560 ug/ml = 10ml x 0.8 ug/ml --- see how I made sure the units were the same I changed the mg/ml to ug/ml V1 = .0005 mls and then I would need 9.9995 mls of diluent to make up my 0.8ug/ml concentration, you can change this to be expressed in microliters by multiplying each number by 1000. So in uls - V1 = 0.5 uls and 9999.5 uls from there you can determine the titer which is your total volume divided by the volume of concentrated antibody 10/.0005 That would be a 1:20,000 dilution For a dilution such as this I would make up a stock solution of either 1:100 or 1:1000 and then dilute from there. Now its time for the rest of you to check my math, did I do this correctly????? I went over it a few times, but you never know.............. Have a GREAT Weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Friday, November 21, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab math: ihc dilution To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Nov 21 14:04:37 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Nov 21 14:04:43 2014 Subject: [Histonet] RE: lab math: ihc dilution In-Reply-To: <9EF5279EBDFE6E4FB6605E8F183A0027D154A5D3@CVM76.vetmed.wsu.edu> References: <14E2C6176416974295479C64A11CB9AE019C79ECDC9C@SBS2K8.premierlab.local> <546F5F3D0200008B00054C43@oes-grpwise.novell.upei.ca> <9EF5279EBDFE6E4FB6605E8F183A0027D154A5D3@CVM76.vetmed.wsu.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDCA0@SBS2K8.premierlab.local> You are right I got it wrong, see no matter how many time you run the math, you still come up with problems Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Truscott, Tom [mailto:ttruscot@vetmed.wsu.edu] Sent: Friday, November 21, 2014 1:01 PM To: Kathleen Jones; Sheryl Stephenson; histonet@lists.utsouthwestern.edu; Elizabeth Chlipala Subject: RE: [Histonet] RE: lab math: ihc dilution When I solve your equation V1 X 1560 ug/ml = 10ml X 0.8 ug/ml I get .005 ml. Therefore the dilution would be closer to 1:2000. I would usually divide the 1560 ug/ml by 0.8 ug/ml and get 1950 therefore 1:1950 would be more accurate . Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Jones Sent: Friday, November 21, 2014 11:50 AM To: Sheryl Stephenson; histonet@lists.utsouthwestern.edu; Elizabeth Chlipala Subject: [Histonet] RE: lab math: ihc dilution Hi Liz, Wouldn't you dilute in 999.5ul of diluent, not 9999.5. 1ml=1000ul Otherwise I got the same answer... Kathleen Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)213-2207 >>> Elizabeth Chlipala 21/11/2014 3:35 PM >>> Sheryl Here is how I would approach the problem. We would use the basic mathematical equation of the following (in order for this to work you need to keep the units the same) V1 x C1 = V2 x C2 V1 - volume of stock solution needed - this is what you are solving for C1 - concentration of stock solution - you know this its 1.56 mg/ml or 1560 ug/ml (there are 1000 ug in one mg) V2 - volume of final solution needed - I put an arbitrary volume of 10 mls needed C2 - concentration of final solution - you know this its 0.8ug/ml V1 x 1560 ug/ml = 10ml x 0.8 ug/ml --- see how I made sure the units were the same I changed the mg/ml to ug/ml V1 = .0005 mls and then I would need 9.9995 mls of diluent to make up my 0.8ug/ml concentration, you can change this to be expressed in microliters by multiplying each number by 1000. So in uls - V1 = 0.5 uls and 9999.5 uls from there you can determine the titer which is your total volume divided by the volume of concentrated antibody 10/.0005 That would be a 1:20,000 dilution For a dilution such as this I would make up a stock solution of either 1:100 or 1:1000 and then dilute from there. Now its time for the rest of you to check my math, did I do this correctly????? I went over it a few times, but you never know.............. Have a GREAT Weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Friday, November 21, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab math: ihc dilution To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Fri Nov 21 14:11:24 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Nov 21 14:11:30 2014 Subject: [Histonet] RE: Specimen numbering systems In-Reply-To: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355EDE17@HHCEXCHMB03.hhcsystem.org> We are currently using "Numeric" for the specimen (or part) and "Alpha" for the block. I don't like it; we frequently have "1ZZZZZ" blocks for large CA resections! I would like to change this going forward. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, November 21, 2014 2:03 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Toni.Rathborne <@t> rwjuh.edu Fri Nov 21 14:17:58 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Fri Nov 21 14:18:10 2014 Subject: [Histonet] RE: Specimen numbering systems In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355EDE17@HHCEXCHMB03.hhcsystem.org> References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> <9215BD4B0BA1B44D962A71C758B68D2E355EDE17@HHCEXCHMB03.hhcsystem.org> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24EDD444@vap1014.win.rwjuh.edu> We use a numeric for the accession number (S-14-1234), followed by alpha for the specimen(A, B, C), and within that have numeric for the number of blocks in each specimen (A1, A2, A3). We haven't ever gone to "Z" in our current LIS, and the ability to add unlimited blocks to each specimen makes it easy to deal with. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, November 21, 2014 3:11 PM To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We are currently using "Numeric" for the specimen (or part) and "Alpha" for the block. I don't like it; we frequently have "1ZZZZZ" blocks for large CA resections! I would like to change this going forward. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, November 21, 2014 2:03 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Nov 21 14:27:53 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Nov 21 14:28:01 2014 Subject: [Histonet] RE: Specimen numbering systems In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355EDE17@HHCEXCHMB03.hhcsystem.org> References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> <9215BD4B0BA1B44D962A71C758B68D2E355EDE17@HHCEXCHMB03.hhcsystem.org> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F1310C5@evcspmbx2.ads.northwestern.edu> We do Alpha numeric so A1,A2 but our samples also get barcodes so we have a barcode ID that picks up whatever you designate it to and it is AAA001234 0000 (designating a block) the slides then become AAA001234 0001, AAA001234 0002 etc. ties it back to the block and uniquely identified (called parent and children) The hitch here is if you had, say a colon it would be the 0000 (parent) and each block created would be a child and then technically the slides become the grandchildren. It works, but can be confusing at times - we're still working out the kinks. Turns out NCI and NIH use the system..... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, November 21, 2014 2:11 PM To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We are currently using "Numeric" for the specimen (or part) and "Alpha" for the block. I don't like it; we frequently have "1ZZZZZ" blocks for large CA resections! I would like to change this going forward. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, November 21, 2014 2:03 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Fri Nov 21 14:45:46 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Fri Nov 21 14:45:57 2014 Subject: [Histonet] RE: lab math: ihc dilution In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECDCA0@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECDC9C@SBS2K8.premierlab.local> <546F5F3D0200008B00054C43@oes-grpwise.novell.upei.ca> <9EF5279EBDFE6E4FB6605E8F183A0027D154A5D3@CVM76.vetmed.wsu.edu> <14E2C6176416974295479C64A11CB9AE019C79ECDCA0@SBS2K8.premierlab.local> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A0027D154A614@CVM76.vetmed.wsu.edu> You are so right-that's why I always try to double check everything even my spellink. Tom T -----Original Message----- From: Elizabeth Chlipala [mailto:liz@premierlab.com] Sent: Friday, November 21, 2014 12:05 PM To: Truscott, Tom; 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) Subject: RE: [Histonet] RE: lab math: ihc dilution You are right I got it wrong, see no matter how many time you run the math, you still come up with problems Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Truscott, Tom [mailto:ttruscot@vetmed.wsu.edu] Sent: Friday, November 21, 2014 1:01 PM To: Kathleen Jones; Sheryl Stephenson; histonet@lists.utsouthwestern.edu; Elizabeth Chlipala Subject: RE: [Histonet] RE: lab math: ihc dilution When I solve your equation V1 X 1560 ug/ml = 10ml X 0.8 ug/ml I get .005 ml. Therefore the dilution would be closer to 1:2000. I would usually divide the 1560 ug/ml by 0.8 ug/ml and get 1950 therefore 1:1950 would be more accurate . Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Jones Sent: Friday, November 21, 2014 11:50 AM To: Sheryl Stephenson; histonet@lists.utsouthwestern.edu; Elizabeth Chlipala Subject: [Histonet] RE: lab math: ihc dilution Hi Liz, Wouldn't you dilute in 999.5ul of diluent, not 9999.5. 1ml=1000ul Otherwise I got the same answer... Kathleen Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)213-2207 >>> Elizabeth Chlipala 21/11/2014 3:35 PM >>> Sheryl Here is how I would approach the problem. We would use the basic mathematical equation of the following (in order for this to work you need to keep the units the same) V1 x C1 = V2 x C2 V1 - volume of stock solution needed - this is what you are solving for C1 - concentration of stock solution - you know this its 1.56 mg/ml or 1560 ug/ml (there are 1000 ug in one mg) V2 - volume of final solution needed - I put an arbitrary volume of 10 mls needed C2 - concentration of final solution - you know this its 0.8ug/ml V1 x 1560 ug/ml = 10ml x 0.8 ug/ml --- see how I made sure the units were the same I changed the mg/ml to ug/ml V1 = .0005 mls and then I would need 9.9995 mls of diluent to make up my 0.8ug/ml concentration, you can change this to be expressed in microliters by multiplying each number by 1000. So in uls - V1 = 0.5 uls and 9999.5 uls from there you can determine the titer which is your total volume divided by the volume of concentrated antibody 10/.0005 That would be a 1:20,000 dilution For a dilution such as this I would make up a stock solution of either 1:100 or 1:1000 and then dilute from there. Now its time for the rest of you to check my math, did I do this correctly????? I went over it a few times, but you never know.............. Have a GREAT Weekend! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Friday, November 21, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] lab math: ihc dilution To all you math whiz out there, please help with this math dilution. If my Ab conc is 1.56 mg/mL and they want to optimize to 0.8 ug/mL, what dilution should I use? Thanks, Sheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri Nov 21 15:00:39 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri Nov 21 15:00:56 2014 Subject: [Histonet] Specimen numbering systems In-Reply-To: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> Message-ID: <494885073.4206855.1416603639109.JavaMail.zimbra@comcast.net> We use?a numeric for the case number starting with the year 14-SR-1111 and an alpha and number for blocs with the case.? A1, A2, A3 so it looks like 14-SR-1111 A1 and the next A block is A2 and so on with as many?alpha designations as needed.? Last week we had one surgical case with A through AA so it is somewhat flexible that way.? Pam Marcum UAMS ----- Original Message ----- From: "Donna G. Willis" To: "Histonet" Sent: Friday, November 21, 2014 1:02:56 PM Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. ?Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas ?75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Fri Nov 21 15:15:12 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Nov 21 15:15:18 2014 Subject: [Histonet] Fast Green In-Reply-To: <5A590EB108038B4FA01F8CDB39C6541B05614DB1@EXCMBXPW7.chop.edu> References: <5A590EB108038B4FA01F8CDB39C6541B05614DB1@EXCMBXPW7.chop.edu> Message-ID: Try 70% ETOH, leave the slides to soak for a good while. If that doesn't work after a couple of hours, try 1% HCl in 70% ETOH. Potassium permanganate followed by oxalic acid takes out just about anything, in my experience, but it's pretty harsh (1% aq potassium permanganate, 2% aq oxalic acid). Just like cleaning a stain on a shirt, you always want to start with the weakest solution first, and work your way up if necessary. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Nov 21, 2014 at 12:49 PM, Berger, Rebecca wrote: > Hey there, does anyone have a good method to de-stain Fast green? The > slides need to be re-stained with H&E. > Thanks. > > > > > Rebecca Berger, HT(ASCP)CM > Research Technician > Division of Orthopedic Surgery > The Children's Hospital of Philadelphia Research Institute > 3615 Civic Center Boulevard, ARC904 (Lab) > Philadelphia, PA 19104 > Phone: 267-425-2076 Email: bergerr1@email.chop.edu bergerr1@email.chop.edu> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Reuel.Cornelia <@t> tsrh.org Fri Nov 21 17:20:06 2014 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Fri Nov 21 17:20:11 2014 Subject: [Histonet] Reference to microtome micrometer thickness Message-ID: <546F7446.0F7E.00C5.1@tsrh.org> Hello Histonetters, how will we verify the thickness of a paraffin section in a slide. Do we have a reference regarding on how to measure the thickness. I based the thickness of my section thru the mcirometer on the rotary microtome but one of our reveiwers does not believed that we are cutting them into 3 micron thickness or 4 micron thickness. Please if we have any reference, please share it to me. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From tejohnson <@t> genoptix.com Fri Nov 21 18:24:11 2014 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Fri Nov 21 18:24:25 2014 Subject: [Histonet] Re: lab math: ihc dilution Message-ID: <0498a36719334d38a707cb8382af9fcd@PHUSCB-SP37MB03.genoptix.org> Here's how I approach this problem. Convert all concentrations into the same units. I prefer to use whichever the final concentration unit is, so it looks like this: 1.56 mg/ml = 1560 ug/ml You have 1560 ug/ml and you need 0.8 ug/ml Divide 1560 by 0.8 = 1950. So your final dilution is 1:1950. Pipetting 1 ul of antibody in a dilution isn't very accurate, and using the above as 1 ul antibody in 1949 ul of diluent would yield you almost 2 ml of diluted antibody. Not a problem if you are doing batch staining where you might need 5 or 10 ml of diluted antibody. But if you are staining only 1 or 2 slides, you might not want to make that much. So follow Liz's advice about starting with a 1:10 dilution. Short division (move the decimal point one place to the left) puts the concentration at 156.0 ug/ml. 156 divided by 0.8 - 195 (You can see from above you could have just moved the final dilution decimal point one place to the left to get the same answer) With a 1:10 dilution of stock your final dilution would be 1:195 and that seems a bit more reasonable for fewer slides/total volume. Remember that 1:195 dilution is 1 part of antibody added to 194 parts of diluent. I would then multiply each side by the same number to get roughly how ever much diluted antibody you need. You can make *EXACTLY* however much you need, but that requires more complicated math, and I don't mind making just slightly over what I need to account for pipetting loss. Ex: If I need 100 ul/slide of diluted antibody and 4 slides to stain, I would multiply both sides by 3, since multiplying it by 2 won't give you quite enough : 1 x 3 = 3 (ul of 1:10 antibody) 194 x 3 = 582 (ul of diluent) Not sure if I helped or confused things. Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From JMacDonald <@t> mtsac.edu Fri Nov 21 20:09:48 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Nov 21 20:16:43 2014 Subject: [Histonet] Help with muscle Message-ID: Hello All, I hope I am not asking a dumb question. As I know that everyone on the histonet is very knowledgeable I was hoping to get some suggestions regarding processing tissue for neuromuscular junction staining. We are a neuro research lab and want to quantify neuromuscular junction on treated and non-treated rat gastrocnemius. We need the whole muscle for quantifications. The first problem we encountered when attempting to do this on PFA perfused and post fixed whole muscle was negative staining with SV2 antibody (presynaptic) but nicely stained alpha bungarotoxin (post synaptic) end plates. After a few attempts with no success we decided to freeze the whole muscle in dry ice and isopentane. First off we are having issues with the middle of the muscle freezing completely even after leaving it in solution for more than 10 min. Secondly while we do get nice staining with SV2 and bungarotoxin in some of the endplates we don?t see colocalization in most of the NMJ?s. One possibility that I was thinking could be causing this is that when the muscle is being picked up on the slide (cryosections) it is not laying completely flat on the slide (we tend to have to focus back and forth quite a bit) so we see the SV2/bungarotoxin near each other but not overlapping. One other thing I thought might be occurring is that when the muscle is being frozen it is retracting causing a shift in the presynaptic/postsynaptic NMJ. What is the best way to process the tissue, fixation or freezing? Any suggestions are greatly appreciated. Thanks, Leslie Leslie Garcia Senior Histologist Clive Svendsen Lab Board of Governors Regenerative Medicine Institute Cedars-Sinai Medical Center 8700 Beverly Blvd. AHSP 8405 Los Angeles, CA 90048 Phone - 310-248-8571 Web - http://www.cedars-sinai.edu/RMI From j.rowaihi <@t> alborglaboratories.com Fri Nov 21 23:59:34 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Sat Nov 22 00:00:08 2014 Subject: [Histonet] Specimen numbering systems In-Reply-To: References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> Message-ID: <024301d00619$7f9e1540$7eda3fc0$@rowaihi@alborglaboratories.com> I am doing the same of Joelle Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver Sent: Friday, November 21, 2014 10:30 PM To: Willis, Donna G.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Specimen numbering systems Numeric for the Specimen and Alpha for the Block. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Donna.Willis@baylorhealth.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 21 Nov 2014 19:02:56 +0000 > Subject: [Histonet] Specimen numbering systems > > Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. > > Thanks, > > Donna Willis, HT/HTL(ASCP) > Anatomic Pathology Manager > > Baylor University Medical Center > 3500 Gaston Ave|Dallas, Texas 75246 > 214-820-2465 office|214-725-6184 mobile > BaylorScottandWhite.com > > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Sat Nov 22 11:30:16 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Sat Nov 22 11:30:26 2014 Subject: [Histonet] Reference to microtome micrometer thickness In-Reply-To: <546F7446.0F7E.00C5.1@tsrh.org> References: <546F7446.0F7E.00C5.1@tsrh.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDCAA@SBS2K8.premierlab.local> Reuel When your microtomes are serviced yearly they should be calibrated at the same time for micron thickness. We have Leica microtomes and they are serviced by one of their vendors. I had actually asked our service technician if they check for that and he said that do that, he also pointed out that your knife angle can also affect section thickness. But that is just calibrating the microtome. Overall section thickness can vary dependent upon many variables. The type of disposable blade you use, how sharp that blade is, how many blocks you have sectioned with that particular blade, how cold your block is, how fast or slow you turn the wheel of your microtome, some people blow on the blocks, which we all know makes sectioning easier, because it warms up the block and therefore you will get a thicker section, all of those parameters can affect your micron thickness. Slight variation in section thickness may not impact routine H&E slides for diagnosis, etc. But when you are running IHC, or any stain and then utilizing image analysis that's where section thickness consistency is very important. If a section is too thick you will be able to focus through multiple planes of nuclei in the sample, meaning you should see only a single cell layer when tissue has been cut at the proper thickness. I hope this helps. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: Friday, November 21, 2014 4:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reference to microtome micrometer thickness Hello Histonetters, how will we verify the thickness of a paraffin section in a slide. Do we have a reference regarding on how to measure the thickness. I based the thickness of my section thru the mcirometer on the rotary microtome but one of our reveiwers does not believed that we are cutting them into 3 micron thickness or 4 micron thickness. Please if we have any reference, please share it to me. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From Catrina.Corral <@t> tmmc.com Sat Nov 22 12:00:33 2014 From: Catrina.Corral <@t> tmmc.com (Corral, Catrina D.) Date: Sat Nov 22 12:00:45 2014 Subject: [Histonet] Possible dispensing problems Message-ID: <36B17DD18E14244CB085DBE3A132C0E715A591D2@EXCH-3.tmmc.com> Hello, We are having issues with stains not working on one or two slides on a case with multiple blocks. On first glance it appears as if the dispenser did not dispense, but nothing appears to be wrong with the dispenser. It happens on random slides with no real definable reason. Has anyone else had this issue? Any information is appreciated. From carl.hobbs <@t> kcl.ac.uk Sat Nov 22 13:48:14 2014 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Nov 22 13:50:06 2014 Subject: [Histonet] RE: lab math: ihc dilution Message-ID: <1416685690890.42474@kcl.ac.uk> ?Imho, the Ab concentration is irrelevant. I consider dilution factors more important. You can have a 1mg/ml Ab conc....yet it may not work in any particular application. Think affinity/avidity.....these are critical. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From Maxim_71 <@t> mail.ru Sat Nov 22 20:37:28 2014 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Nov 22 20:37:50 2014 Subject: [Histonet] Reference to microtome micrometer thickness Message-ID: <1529659932.20141123053728@mail.ru> Reuel, It is right, that there are many external reasons for a thickness of paraffin sections. It is possible to measure the final stained sections with mathematical methods by slide scanner. I hope that the slide-scanner can be easily to calculate thickness of section by their soft. Each individually sections will have own specific thickness. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From dmlaud <@t> laudierhistology.com Sun Nov 23 07:50:38 2014 From: dmlaud <@t> laudierhistology.com (Damien) Date: Sun Nov 23 07:50:42 2014 Subject: [Histonet] embedding of insects in historesin (Technovit 7100) In-Reply-To: References: Message-ID: Hi Jurgen, If you still need assistance,please let me know. Feel free to contact me directly. Best, Damien L. On Fri, Nov 21, 2014 at 12:55 PM, wrote: > > Hallo, Andi G. > > Thanks for your reply. Normally I use paraffine for embedding insects > after dehydration and using N Butylalkohol with sucess. In my opinion > it is absolutly necessary to avoid any rests of water and to cut cool. > But this technique is limited to sections of 3-4 ? and heating is > unavoidable. > I would be very grateful for a reply of Damien Laudier or of someone > else, who could help me in this matter. > > J?rgen > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Damien Laudier Laudier Histology www.LaudierHistology.com From pdefazio802 <@t> gmail.com Sun Nov 23 12:19:45 2014 From: pdefazio802 <@t> gmail.com (Pam DeFazio) Date: Sun Nov 23 12:19:51 2014 Subject: [Histonet] CPT code question Message-ID: We use a nail softening solution to soften toenails submitted for possible fungus. Is there a CPT code for this or can I use the decalcification code? Thanks! Pam ARMC Athens, Ga From jeribaker10 <@t> gmail.com Sun Nov 23 13:05:12 2014 From: jeribaker10 <@t> gmail.com (Jeryl Baker) Date: Sun Nov 23 13:05:19 2014 Subject: [Histonet] CPT code question In-Reply-To: References: Message-ID: <451E1AA4-B46C-4696-95B7-27FE94C8538B@gmail.com> FYI Pam, hydrogen peroxide works great, if you soak the nail in that prior to embedding, face the block and then soak the block prior to sectioning. Sent from my iPhone > On Nov 23, 2014, at 12:19 PM, Pam DeFazio wrote: > > We use a nail softening solution to soften toenails submitted for possible > fungus. Is there a CPT code for this or can I use the decalcification code? > Thanks! Pam > ARMC > Athens, Ga > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Sun Nov 23 16:22:04 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Nov 23 16:22:45 2014 Subject: [Histonet] Intestinal mucus -- Carnoy or what? In-Reply-To: <546F1299.3030809@helsinki.fi> References: <852F7D2C14FB464D80E182B15DB138AF394FE140@CHIEX005.CHI.catholichealth.net> <546F1299.3030809@helsinki.fi> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53CC055@xmdb04.nch.kids> Since the mucus will tend to be water soluble. I would suggest 10% formaldehyde in alcohol. Fix for the usual time, dependant on size. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mikael Niku Sent: Friday, 21 November 2014 9:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Intestinal mucus -- Carnoy or what? Hello! What is the best way to fix intestinal samples in order to preserve the mucus (and the embedded bacteria)? I was recommended Carnoy's, but are there any alternatives... perhaps without chloroform? With best regards, Mikael _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From hans <@t> histologistics.com Mon Nov 24 08:50:55 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Mon Nov 24 08:51:01 2014 Subject: [Histonet] Staining for plant peroxidase Message-ID: Hello All, We are in need of help finding sources for plant peroxidase staining. If someone could point me in the right direction, a link, papers or books, I am happy to research. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From bburnett <@t> CapeCodHealth.org Mon Nov 24 12:00:28 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Mon Nov 24 12:00:43 2014 Subject: [Histonet] HER2 scoring exercises for breast cases Message-ID: Does anyone know of a good website for practicing HER2 scoring on breast tissue? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From rachel <@t> gbi-inc.com Mon Nov 24 13:02:16 2014 From: rachel <@t> gbi-inc.com (Rachel Gonzalez) Date: Mon Nov 24 13:02:25 2014 Subject: [Histonet] N-Cadherin Antibody for FFPE IHC Message-ID: <00c701d00819$2a192540$7e4b6fc0$@gbi-inc.com> Hi I just joined the histonet (3 days). so I am not sure of the appropriateness of this question but I would really appreciate the help. Does anyone know of an N-cadherin antibody that works on paraffin embedded tissue. I have a deadline in the next few weeks to get the staining done. We have one in house that is working beautifully but the project requires a second N-Cadherin antibody. For the second one I have tried Spring Biosciences N-Cadherin M3900 and I am getting no staining (Kidney, Carcinoid, Glioma). I used Spring Bioscience protocol exactly and 9 variations of their protocol. I used a titer of 1:10 and all I get is background staining in mostly fluids in vessels, cysts and tubules. There is no membrane staining that the other antibody is giving. Talking to Spring Biosciences the customer service person (Jennifer W----) stated this antibody only works on mesothelioma. I am not sure that I want an antibody that only works on one tissue especially since my goal is to stain carcinoid. I was hoping for a few recommendations to help narrow the scope. Thanks, RMG From hymclab.hymclab <@t> ministryhealth.org Mon Nov 24 13:26:24 2014 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Mon Nov 24 13:26:41 2014 Subject: [Histonet] RE: Specimen numbering systems In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24EDD444@vap1014.win.rwjuh.edu> References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> <9215BD4B0BA1B44D962A71C758B68D2E355EDE17@HHCEXCHMB03.hhcsystem.org> <59E09A4EFBD3F349BD75FDAE8AFB0F24EDD444@vap1014.win.rwjuh.edu> Message-ID: We do it the same as Toni and from the CAP Uniform Labeling of slides and Blocks presentation I attended at NSH that is what CAP came up with for uniform labeling (S14-1234 A1, A2, A3, B1, B2, etc...). Dawn Schneider, HT(ASCP) Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54568 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, November 21, 2014 2:18 PM To: 'Cartun, Richard'; Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We use a numeric for the accession number (S-14-1234), followed by alpha for the specimen(A, B, C), and within that have numeric for the number of blocks in each specimen (A1, A2, A3). We haven't ever gone to "Z" in our current LIS, and the ability to add unlimited blocks to each specimen makes it easy to deal with. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, November 21, 2014 3:11 PM To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We are currently using "Numeric" for the specimen (or part) and "Alpha" for the block. I don't like it; we frequently have "1ZZZZZ" blocks for large CA resections! I would like to change this going forward. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, November 21, 2014 2:03 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Ryan.Roy <@t> va.gov Mon Nov 24 13:42:07 2014 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Mon Nov 24 13:42:35 2014 Subject: [Histonet] RE: Specimen numbering systems In-Reply-To: References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> <9215BD4B0BA1B44D962A71C758B68D2E355EDE17@HHCEXCHMB03.hhcsystem.org> <59E09A4EFBD3F349BD75FDAE8AFB0F24EDD444@vap1014.win.rwjuh.edu> Message-ID: <15F883394EAB744E99E1C7E1B98730490176EF4E7167@R04BYNMSGB1.r04.med.va.gov> I agree with Dawn . Basically use a numeric for the Block and an Alpha for the specimen. This way a large resection specimen would receive an A and all blocks for said specimen would be labeled as A1,A2,A3...ect -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Monday, November 24, 2014 2:26 PM To: 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [EXTERNAL] [Histonet] RE: Specimen numbering systems We do it the same as Toni and from the CAP Uniform Labeling of slides and Blocks presentation I attended at NSH that is what CAP came up with for uniform labeling (S14-1234 A1, A2, A3, B1, B2, etc...). Dawn Schneider, HT(ASCP) Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54568 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, November 21, 2014 2:18 PM To: 'Cartun, Richard'; Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We use a numeric for the accession number (S-14-1234), followed by alpha for the specimen(A, B, C), and within that have numeric for the number of blocks in each specimen (A1, A2, A3). We haven't ever gone to "Z" in our current LIS, and the ability to add unlimited blocks to each specimen makes it easy to deal with. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, November 21, 2014 3:11 PM To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We are currently using "Numeric" for the specimen (or part) and "Alpha" for the block. I don't like it; we frequently have "1ZZZZZ" blocks for large CA resections! I would like to change this going forward. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, November 21, 2014 2:03 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mshaughnessy <@t> GENERAL-DATA.com Mon Nov 24 13:58:56 2014 From: mshaughnessy <@t> GENERAL-DATA.com (Shaughnessy, Mary) Date: Mon Nov 24 13:59:09 2014 Subject: [Histonet] Re: Histonet Digest, Vol 132, Issue 26 In-Reply-To: <7211b7ff-4d3d-47c5-9f50-5022f357610b@newman.general-data.com> References: <7211b7ff-4d3d-47c5-9f50-5022f357610b@newman.general-data.com> Message-ID: <37F3AC4C-C4B2-43E2-8818-B65E4486F364@GENERAL-DATA.com> thanks for the heads up. Let me look into this. Mary Morrow (Shaughnessy) Southwest Regional Business Development Manager General Data Healthcare Mshaughnessy@general-data.com 480-291-2757 Sent from my iPad > On Nov 24, 2014, at 11:02 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. CPT code question (Pam DeFazio) > 2. Re: CPT code question (Jeryl Baker) > 3. RE: Intestinal mucus -- Carnoy or what? (Tony Henwood (SCHN)) > 4. Staining for plant peroxidase (Hans B Snyder) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 23 Nov 2014 13:19:45 -0500 > From: Pam DeFazio > Subject: [Histonet] CPT code question > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > We use a nail softening solution to soften toenails submitted for possible > fungus. Is there a CPT code for this or can I use the decalcification code? > Thanks! Pam > ARMC > Athens, Ga > > > ------------------------------ > > Message: 2 > Date: Sun, 23 Nov 2014 13:05:12 -0600 > From: Jeryl Baker > Subject: Re: [Histonet] CPT code question > To: Pam DeFazio > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: <451E1AA4-B46C-4696-95B7-27FE94C8538B@gmail.com> > Content-Type: text/plain; charset=us-ascii > > FYI Pam, hydrogen peroxide works great, if you soak the nail in that prior to embedding, face the block and then soak the block prior to sectioning. > > Sent from my iPhone > >> On Nov 23, 2014, at 12:19 PM, Pam DeFazio wrote: >> >> We use a nail softening solution to soften toenails submitted for possible >> fungus. Is there a CPT code for this or can I use the decalcification code? >> Thanks! Pam >> ARMC >> Athens, Ga >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Sun, 23 Nov 2014 22:22:04 +0000 > From: "Tony Henwood (SCHN)" > Subject: RE: [Histonet] Intestinal mucus -- Carnoy or what? > To: "'Mikael Niku'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53CC055@xmdb04.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Since the mucus will tend to be water soluble. I would suggest 10% formaldehyde in alcohol. > Fix for the usual time, dependant on size. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mikael Niku > Sent: Friday, 21 November 2014 9:23 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Intestinal mucus -- Carnoy or what? > > Hello! > > What is the best way to fix intestinal samples in order to preserve the mucus (and the embedded bacteria)? > I was recommended Carnoy's, but are there any alternatives... perhaps without chloroform? > > With best regards, > Mikael > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 4 > Date: Mon, 24 Nov 2014 09:50:55 -0500 > From: Hans B Snyder > Subject: [Histonet] Staining for plant peroxidase > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hello All, > > We are in need of help finding sources for plant peroxidase staining. If > someone could point me in the right direction, a link, papers or books, I > am happy to research. > > Thank you > > > Hans B Snyder > Histologistics > 60 Prescott Street > Worcester, MA 01605 > 508-308-7800 > hans@histologistics.com > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 132, Issue 26 > ***************************************** This email may contain confidential General Data Company, Inc. information: any unauthorized or improper disclosure, copying, distribution or use of the contents of this email and attached document(s) is prohibited and may be a criminal offense. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you received this email in error, please reply immediately to the sender & delete this message and the attached document(s) without disclosure. From BAMoe <@t> gundersenhealth.org Mon Nov 24 14:56:26 2014 From: BAMoe <@t> gundersenhealth.org (Moe, Barbi A) Date: Mon Nov 24 14:59:31 2014 Subject: [Histonet] Kidney bx transport solution Message-ID: <8B4B31D17067E540AC760B4A30190B99014A406289@LXEXMB03.gundluth.org> Hi all - Currently our histology lab staff travels to our Interventional Radiology suite when a kidney bx is performed. At the procedure site, the sample is handed directly to the tech, it is evaluated for adequacy, divided, and put into the following solutions - formalin, Trump's, and Zeus Wash solution. Upon return to the laboratory, the cores in Zeus Wash solution are then frozen for immunofluoresence done on site. We are looking into a new way of doing things :-) and considering having the kidney bx delivered to the laboratory after procurement. The samples would be hand-delivered to the lab immediately (within 5 - 10 minutes) and the samples (usually 3-4 cores) would be divided in the histology lab. Could I get an idea of how many labs have the sample delivered to them vs the number that assist at the procedure site? Also, any thoughts on what the best transport media/method would be? All thoughts are much appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundersenhealth.org Barb Moe From Timothy.Morken <@t> ucsf.edu Mon Nov 24 15:10:27 2014 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Mon Nov 24 15:10:36 2014 Subject: [Histonet] RE: Kidney bx transport solution In-Reply-To: <8B4B31D17067E540AC760B4A30190B99014A406289@LXEXMB03.gundluth.org> References: <8B4B31D17067E540AC760B4A30190B99014A406289@LXEXMB03.gundluth.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367B8427@ex07.net.ucsf.edu> Barb, the clinical/nursing staff deliver the bx to our Grossing lab. Grossing takes some for light microscopy and forwards the parts for IF and EM to the IF/EM lab.(we pick up from grossing or they deliver to us). In EM/If we look at the cores and determine the best dissection to make and freeze/fix there. If grossing has a hard time deciding if the cores are good they bring all the specimens to us and we figure it out. Everything comes fresh from the clinician on saline-wetted telfa pads in a 45mm petri dish in an ice container. We make up kits in the EM lab, including 100ml plastic beakers of ice, and they use those kits for the biopsy retrieval. We don't use Zeus for in-house specimens (though we may for some future cases due to a new facility opening that is 30 to 45 min away by courier). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Moe, Barbi A Sent: Monday, November 24, 2014 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney bx transport solution Hi all - Currently our histology lab staff travels to our Interventional Radiology suite when a kidney bx is performed. At the procedure site, the sample is handed directly to the tech, it is evaluated for adequacy, divided, and put into the following solutions - formalin, Trump's, and Zeus Wash solution. Upon return to the laboratory, the cores in Zeus Wash solution are then frozen for immunofluoresence done on site. We are looking into a new way of doing things :-) and considering having the kidney bx delivered to the laboratory after procurement. The samples would be hand-delivered to the lab immediately (within 5 - 10 minutes) and the samples (usually 3-4 cores) would be divided in the histology lab. Could I get an idea of how many labs have the sample delivered to them vs the number that assist at the procedure site? Also, any thoughts on what the best transport media/method would be? All thoughts are much appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundersenhealth.org Barb Moe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> skm.org.pk Mon Nov 24 20:08:17 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Mon Nov 24 20:06:16 2014 Subject: [Histonet] RE: Specimen numbering systems In-Reply-To: <15F883394EAB744E99E1C7E1B98730490176EF4E7167@R04BYNMSGB1.r04.med.va.gov> References: <2572B4D63B62E64A8078D8BBE34D407801BDA296@BHDASVEXML2.bhcs.pvt> <9215BD4B0BA1B44D962A71C758B68D2E355EDE17@HHCEXCHMB03.hhcsystem.org> <59E09A4EFBD3F349BD75FDAE8AFB0F24EDD444@vap1014.win.rwjuh.edu> <15F883394EAB744E99E1C7E1B98730490176EF4E7167@R04BYNMSGB1.r04.med.va.gov> Message-ID: I also agree with Dawn. We shall be start from Jan 2015 as this way. Muhammad Tahseen, MT (JIMTEF) Japan Senior supervisor Histopathology SKMCH&RC Lahore Pakistan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roy, Ryan Sent: Tuesday, November 25, 2014 12:42 AM To: 'hymclab'; 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems I agree with Dawn . Basically use a numeric for the Block and an Alpha for the specimen. This way a large resection specimen would receive an A and all blocks for said specimen would be labeled as A1,A2,A3...ect -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Monday, November 24, 2014 2:26 PM To: 'Rathborne, Toni'; 'Cartun, Richard'; Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [EXTERNAL] [Histonet] RE: Specimen numbering systems We do it the same as Toni and from the CAP Uniform Labeling of slides and Blocks presentation I attended at NSH that is what CAP came up with for uniform labeling (S14-1234 A1, A2, A3, B1, B2, etc...). Dawn Schneider, HT(ASCP) Howard Young Medical Center 240 Maple Ave. Woodruff, WI 54568 715-356-8174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, November 21, 2014 2:18 PM To: 'Cartun, Richard'; Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We use a numeric for the accession number (S-14-1234), followed by alpha for the specimen(A, B, C), and within that have numeric for the number of blocks in each specimen (A1, A2, A3). We haven't ever gone to "Z" in our current LIS, and the ability to add unlimited blocks to each specimen makes it easy to deal with. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, November 21, 2014 3:11 PM To: Willis, Donna G.; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: Specimen numbering systems We are currently using "Numeric" for the specimen (or part) and "Alpha" for the block. I don't like it; we frequently have "1ZZZZZ" blocks for large CA resections! I would like to change this going forward. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Friday, November 21, 2014 2:03 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Specimen numbering systems Can large facilities of more than 500 beds please let me know how they are numbering their Surgical specimens. Alpha for the Specimens and numeric for the Block or Numeric for the Specimen and Alpha for the Block. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arunjyothisp <@t> gmail.com Tue Nov 25 00:52:47 2014 From: arunjyothisp <@t> gmail.com (Arun Jyothi S.P) Date: Tue Nov 25 00:53:21 2014 Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek In-Reply-To: References: Message-ID: Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait From j.rowaihi <@t> alborglaboratories.com Tue Nov 25 02:18:44 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Tue Nov 25 02:19:44 2014 Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek In-Reply-To: References: Message-ID: <00df01d00888$6fc350b0$4f49f210$@rowaihi@alborglaboratories.com> Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Tuesday, November 25, 2014 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a_schade <@t> conradweiser.org Tue Nov 25 09:29:27 2014 From: a_schade <@t> conradweiser.org (Schade, Adelle) Date: Tue Nov 25 09:29:34 2014 Subject: [Histonet] fixative Message-ID: Hello, I am considering the following fixatives for a high school histology experiment and I would appreciate any input considering safety, disposal, etc. 1- Ultrum II (American MasterTech): promotes disposal in local sewer? 2- Excell Plus (American MasterTech): low-hazard but any idea on disposal from those who use it? 3- Histochoice Tissue Fixative Anyone using these products/ advice is greatly appreciated! Ms. Adelle L. Schade, B.S., M.Ed. Anatomy and Physiology Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA 19551 610-693-8599 x6736 a_schade@conradweiser.org From wdesalvo.cac <@t> outlook.com Tue Nov 25 09:45:52 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Nov 25 09:46:03 2014 Subject: [Histonet] fixative In-Reply-To: References: Message-ID: What are you going to accomplish? Alcohol will work as a fixative and dehydration solution. Less of a disposal issue. William DeSalvo william.desalvo@sonoraquest.ccom 602-768-3692 Sent from my iPhone > On Nov 25, 2014, at 8:30 AM, Schade, Adelle wrote: > > Hello, > I am considering the following fixatives for a high school histology experiment and I would appreciate any input considering safety, disposal, etc. > > > 1- Ultrum II (American MasterTech): promotes disposal in local sewer? > > 2- Excell Plus (American MasterTech): low-hazard but any idea on disposal from those who use it? > > 3- Histochoice Tissue Fixative > > Anyone using these products/ advice is greatly appreciated! > > Ms. Adelle L. Schade, B.S., M.Ed. > Anatomy and Physiology > Conrad Weiser High School > 44 Big Spring Rd. > Robesonia, PA 19551 > 610-693-8599 x6736 > a_schade@conradweiser.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 25 10:00:15 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 25 10:00:22 2014 Subject: [Histonet] fixative In-Reply-To: References: Message-ID: <2039206552.514675.1416931215655.JavaMail.yahoo@jws100159.mail.ne1.yahoo.com> I think the best you should do is obtaining the MSDS for those fixatives and check their composition and disposal.Ren? J. On Tuesday, November 25, 2014 10:30 AM, "Schade, Adelle" wrote: Hello, I am considering the following fixatives for a high school histology experiment and I would appreciate any input considering safety, disposal, etc. 1-? ? ? Ultrum II (American MasterTech):? promotes disposal in local sewer? 2-? ? ? Excell Plus (American MasterTech):? low-hazard but any idea on disposal from those who use it? 3-? ? ? Histochoice Tissue Fixative Anyone using these products/ advice is greatly appreciated! Ms. Adelle L. Schade, B.S., M.Ed. Anatomy and Physiology Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA? 19551 610-693-8599 x6736 a_schade@conradweiser.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ryan.Roy <@t> va.gov Tue Nov 25 10:49:06 2014 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Tue Nov 25 10:52:51 2014 Subject: [EXTERNAL] Re: [Histonet] fixative In-Reply-To: References: Message-ID: <15F883394EAB744E99E1C7E1B98730490176EF4E7168@R04BYNMSGB1.r04.med.va.gov> I agree that alcohol is also less toxic. What type of tissue are you fixing? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, November 25, 2014 10:46 AM To: Schade, Adelle Cc: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] fixative What are you going to accomplish? Alcohol will work as a fixative and dehydration solution. Less of a disposal issue. William DeSalvo william.desalvo@sonoraquest.ccom 602-768-3692 Sent from my iPhone > On Nov 25, 2014, at 8:30 AM, Schade, Adelle wrote: > > Hello, > I am considering the following fixatives for a high school histology experiment and I would appreciate any input considering safety, disposal, etc. > > > 1- Ultrum II (American MasterTech): promotes disposal in local sewer? > > 2- Excell Plus (American MasterTech): low-hazard but any idea on disposal from those who use it? > > 3- Histochoice Tissue Fixative > > Anyone using these products/ advice is greatly appreciated! > > Ms. Adelle L. Schade, B.S., M.Ed. > Anatomy and Physiology > Conrad Weiser High School > 44 Big Spring Rd. > Robesonia, PA 19551 > 610-693-8599 x6736 > a_schade@conradweiser.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Tue Nov 25 10:52:55 2014 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Nov 25 10:53:03 2014 Subject: [Histonet] Kidney bx transport Message-ID: <5F06C3AD0B27264CA20CFA986C87882EDE191D37@EXCHANGEPV3.KGH.ON.CA> We receive kidney biopsies brought directly to our grossing area within a few minutes after the cores are obtained. Previously, this was done by nephrologists, but since the renal biopsies are performed in radiology, the interventional radiologist brings them. We decide adequacy first, then apportion specimen to EM, IF and formalin. Similar to the protocol Tim has mentioned, our cores are received on saline-soaked pads in a petri dish. We transfer them to a slide wetted with saline to assess under light microscopy. It seems to come down to a decision of who wants to go where :) The IVR folks are willing to bring the biopsies to our laboratory - they get instant feedback, and often use the exchange for teaching purposes as we are a tertiary care academic centre. Another factor would be the location of microscope used for visualizing adequacy of the cores. The distance between the two locations is not huge, and there is always an MLT instantly available to assess the cores. Rarely has this system had hiccups. Occasionally, the adequacy is a question-mark, and we err on the side of asking IVR to obtain more tissue. This is usually delivered within 5-10 minutes after we request it. Hope this helps, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From a_schade <@t> conradweiser.org Tue Nov 25 11:03:01 2014 From: a_schade <@t> conradweiser.org (Schade, Adelle) Date: Tue Nov 25 11:03:07 2014 Subject: [EXTERNAL] Re: [Histonet] fixative In-Reply-To: <15F883394EAB744E99E1C7E1B98730490176EF4E7168@R04BYNMSGB1.r04.med.va.gov> References: <15F883394EAB744E99E1C7E1B98730490176EF4E7168@R04BYNMSGB1.r04.med.va.gov> Message-ID: Some of our samples are from preserved specimen. We have a significant number of students that work/live on farms as well so from time to time we get a chick embryo that did not survive. Also, we have researched and would like to try some plant/seed histology processes. If alcohol will produce similar results without excessive dehydration that would be a great option for this setting. Thanks for the input Adelle -----Original Message----- From: Roy, Ryan [mailto:Ryan.Roy@va.gov] Sent: Tuesday, November 25, 2014 11:49 AM To: 'WILLIAM DESALVO'; Schade, Adelle Cc: histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] Re: [Histonet] fixative I agree that alcohol is also less toxic. What type of tissue are you fixing? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, November 25, 2014 10:46 AM To: Schade, Adelle Cc: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] fixative What are you going to accomplish? Alcohol will work as a fixative and dehydration solution. Less of a disposal issue. William DeSalvo william.desalvo@sonoraquest.ccom 602-768-3692 Sent from my iPhone > On Nov 25, 2014, at 8:30 AM, Schade, Adelle wrote: > > Hello, > I am considering the following fixatives for a high school histology experiment and I would appreciate any input considering safety, disposal, etc. > > > 1- Ultrum II (American MasterTech): promotes disposal in local sewer? > > 2- Excell Plus (American MasterTech): low-hazard but any idea on disposal from those who use it? > > 3- Histochoice Tissue Fixative > > Anyone using these products/ advice is greatly appreciated! > > Ms. Adelle L. Schade, B.S., M.Ed. > Anatomy and Physiology > Conrad Weiser High School > 44 Big Spring Rd. > Robesonia, PA 19551 > 610-693-8599 x6736 > a_schade@conradweiser.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Nov 25 11:29:06 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Nov 25 11:29:14 2014 Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek In-Reply-To: <00df01d00888$6fc350b0$4f49f210$@rowaihi@alborglaboratories.com> References: <00df01d00888$6fc350b0$4f49f210$@rowaihi@alborglaboratories.com> Message-ID: There may be a leaking valve. From: "Jamal" To: "'Arun Jyothi S.P'" , Date: 11/25/2014 12:21 AM Subject: RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Sent by: histonet-bounces@lists.utsouthwestern.edu Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Tuesday, November 25, 2014 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Tue Nov 25 12:17:17 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Nov 25 12:17:26 2014 Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek In-Reply-To: References: Message-ID: Not sure if it's the same scenario, but this happened to us once about a dozen years ago on an older version of a VIP. Luckily, I haven't seen this again! One of our lab assistants loaded a basket of formalin-fixed tissues onto a processor that hadn't yet been through the clean cycle. The machine wouldn't let him start the process, so he took the basket out and put it back into formalin to wait while he ran the clean cycle. For that brief moment that the basket was in the retort, a bunch of residual formalin drained off the tissues and basket and mixed in with the residual molten paraffin that was left in the bottom of the retort. Anyway, after he ran the clean cycle he started the run as usual. 8 hours later, our blocks smelled like formalin when we cut them, and they were all "mushy" in texture. So Sakura told us that whenever you run the clean cycle, the VIP (again, it was a really old model and I'm not sure if the technology is still the same) will attempt to draw back into the last station that was used, any residual reagent that was left in the retort before proceeding to the next reagent. Since the last reagent had been paraffin, anything in the retort (including the newly mixed in formalin) went back into the last paraffin chamber on the VIP. As such, the next basket of tissues were infiltrated with formalin-infused paraffin in the very last processing step. If I recall, we had to reprocess almost all of those blocks. Hope this helps. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Monday, November 24, 2014 10:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From jmoreira <@t> sidra.org Tue Nov 25 13:46:51 2014 From: jmoreira <@t> sidra.org (Joana Moreira) Date: Tue Nov 25 13:47:06 2014 Subject: [Histonet] RE: HER2 scoring exercises for breast cases Message-ID: Hi! I found the Leica Bond Oracle HER2 IHC e-Learning quite informative. You can find it at the bottom of the following page: http://www.leicabiosystems.com/ihc-ish/novocastra-reagents/theranostics/details/product/leica-oracle-her2-bond-ihc-system-2/ And if you're interested the Leica BOND HER2 FISH e-Learning course is good too. Joana Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From tony.henwood <@t> health.nsw.gov.au Tue Nov 25 17:37:42 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Nov 25 17:38:02 2014 Subject: [Histonet] RE: Kidney bx transport In-Reply-To: <5F06C3AD0B27264CA20CFA986C87882EDE191D37@EXCHANGEPV3.KGH.ON.CA> References: <5F06C3AD0B27264CA20CFA986C87882EDE191D37@EXCHANGEPV3.KGH.ON.CA> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53CCB1D@xmdb04.nch.kids> Try a cell culture solution like Hanks. It preserves better than normal saline (which despite its name is not isotonic with cells). It will keep a renal and skin biopsy quite well for at least an hour. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Wednesday, 26 November 2014 3:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney bx transport We receive kidney biopsies brought directly to our grossing area within a few minutes after the cores are obtained. Previously, this was done by nephrologists, but since the renal biopsies are performed in radiology, the interventional radiologist brings them. We decide adequacy first, then apportion specimen to EM, IF and formalin. Similar to the protocol Tim has mentioned, our cores are received on saline-soaked pads in a petri dish. We transfer them to a slide wetted with saline to assess under light microscopy. It seems to come down to a decision of who wants to go where :) The IVR folks are willing to bring the biopsies to our laboratory - they get instant feedback, and often use the exchange for teaching purposes as we are a tertiary care academic centre. Another factor would be the location of microscope used for visualizing adequacy of the cores. The distance between the two locations is not huge, and there is always an MLT instantly available to assess the cores. Rarely has this system had hiccups. Occasionally, the adequacy is a question-mark, and we err on the side of asking IVR to obtain more tissue. This is usually delivered within 5-10 minutes after we request it. Hope this helps, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Tue Nov 25 17:39:28 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Nov 25 17:39:50 2014 Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53CCB35@xmdb04.nch.kids> Also check the last alcohol and xylene. Has someone accidentally replaced these solution with formalin by mistake? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, 26 November 2014 5:17 AM To: Arun Jyothi S.P; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Not sure if it's the same scenario, but this happened to us once about a dozen years ago on an older version of a VIP. Luckily, I haven't seen this again! One of our lab assistants loaded a basket of formalin-fixed tissues onto a processor that hadn't yet been through the clean cycle. The machine wouldn't let him start the process, so he took the basket out and put it back into formalin to wait while he ran the clean cycle. For that brief moment that the basket was in the retort, a bunch of residual formalin drained off the tissues and basket and mixed in with the residual molten paraffin that was left in the bottom of the retort. Anyway, after he ran the clean cycle he started the run as usual. 8 hours later, our blocks smelled like formalin when we cut them, and they were all "mushy" in texture. So Sakura told us that whenever you run the clean cycle, the VIP (again, it was a really old model and I'm not sure if the technology is still the same) will attempt to draw back into the last station that was used, any residual reagent that was left in the retort before proceeding to the next reagent. Since the last reagent had been paraffin, anything in the retort (including the newly mixed in formalin) went back into the last paraffin chamber on the VIP. As such, the next basket of tissues were infiltrated with formalin-infused paraffin in the very last processing step. If I recall, we had to reprocess almost all of those blocks. Hope this helps. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Monday, November 24, 2014 10:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From arunjyothisp <@t> gmail.com Wed Nov 26 00:28:59 2014 From: arunjyothisp <@t> gmail.com (Arun Jyothi S.P) Date: Wed Nov 26 00:29:10 2014 Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157F53CCB35@xmdb04.nch.kids> References: <6D6BD1DE8A5571489398B392A38A7157F53CCB35@xmdb04.nch.kids> Message-ID: Dear all We find the problem The retort was over heating and thats y the condensation also very high When the condensation chamber is full its pumping back the reagent in the condensation chamber to the retort and mixing it with paraffin Thanks for all the support On 26 Nov 2014 02:39, "Tony Henwood (SCHN)" wrote: > Also check the last alcohol and xylene. > Has someone accidentally replaced these solution with formalin by mistake? > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian > Sent: Wednesday, 26 November 2014 5:17 AM > To: Arun Jyothi S.P; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Formalin smell in the last paraffin station in vip > 6 tissue tek > > Not sure if it's the same scenario, but this happened to us once about a > dozen years ago on an older version of a VIP. Luckily, I haven't seen this > again! > > One of our lab assistants loaded a basket of formalin-fixed tissues onto a > processor that hadn't yet been through the clean cycle. The machine > wouldn't let him start the process, so he took the basket out and put it > back into formalin to wait while he ran the clean cycle. For that brief > moment that the basket was in the retort, a bunch of residual formalin > drained off the tissues and basket and mixed in with the residual molten > paraffin that was left in the bottom of the retort. Anyway, after he ran > the clean cycle he started the run as usual. 8 hours later, our blocks > smelled like formalin when we cut them, and they were all "mushy" in > texture. > > So Sakura told us that whenever you run the clean cycle, the VIP (again, > it was a really old model and I'm not sure if the technology is still the > same) will attempt to draw back into the last station that was used, any > residual reagent that was left in the retort before proceeding to the next > reagent. Since the last reagent had been paraffin, anything in the retort > (including the newly mixed in formalin) went back into the last paraffin > chamber on the VIP. As such, the next basket of tissues were infiltrated > with formalin-infused paraffin in the very last processing step. If I > recall, we had to reprocess almost all of those blocks. > > Hope this helps. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of > Pathology and Laboratory Medicine Children's Hospital Los Angeles > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P > Sent: Monday, November 24, 2014 10:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 > tissue tek > > Dear all > After processing in vip 6 the last paraffin has a strong odour of formalin > > Have anybody experienced the same > Any ideas why it is happening. > > Arun > Kuwait > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please contact the sender by reply e-mail and destroy all copies of this > original message. > > --------------------------------------------------------------------- > > > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Sydney > Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Sydney Childrens Hospital's > Network accepts no liability for any consequential damage resulting from > email containing computer viruses. > > ********************************************************************************* > From j.rowaihi <@t> alborglaboratories.com Tue Nov 25 02:15:23 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Wed Nov 26 04:27:44 2014 Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek In-Reply-To: References: Message-ID: <00de01d00887$f757fbd0$e607f370$@rowaihi@alborglaboratories.com> Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Tuesday, November 25, 2014 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barryrittman <@t> gmail.com Wed Nov 26 12:05:53 2014 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Wed Nov 26 12:06:03 2014 Subject: [Histonet] Reference to microtome micrometer thickness In-Reply-To: <1529659932.20141123053728@mail.ru> References: <1529659932.20141123053728@mail.ru> Message-ID: It has been a while since I read articles regarding section thickness but I have some comments: 1. On Sat, Nov 22, 2014 at 8:37 PM, Maxim Peshkov wrote: > Reuel, > It is right, that there are many external reasons for a thickness of > paraffin sections. > It is possible to measure the final stained sections with mathematical > methods by slide scanner. > I hope that the slide-scanner can be easily to calculate thickness of > section by their soft. > Each individually sections will have own specific thickness. > > Sincerely, > Maxim Peshkov, > Russia, > Taganrog. > mailto:Maxim_71@mail.ru > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From twheelock <@t> mclean.harvard.edu Wed Nov 26 14:48:00 2014 From: twheelock <@t> mclean.harvard.edu (Wheelock, Timothy R.) Date: Wed Nov 26 14:48:06 2014 Subject: [Histonet] Congo Red problems Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9773C9601@PHSX10MB11.partners.org> Hi All: Lately, I have been having problems with my Congo Red staining too lightly. The Bielschowsky silver stain shows lots of amyloid in the cerebral blood vessels and the cores of the senile plaques. However, the Congo Red stain is pretty faint, although you can still see it. I have just bought a new bottle of Congo red from Sigma, in case it was the particular batch of dye. My protocol, which I have always used, is as follows: 1. Deparaffinize sections and bring down to 95% ethanol 2. Stain in 1% Congo Red solution for 10 minutes. 3. Differentiate in Potassium Hydroxide solution; 3 dips 4. Rinse in 4-5 changes of tap water. 5. Counter-stain in Harris Hematoxylin for 1 minute. 6. Differentiate in Acid Alcohol; 10 dips or so. 7. Blue Hematoxylin in running tap water for 10 minutes. 8. 95% ethanol for only 3 dips, then right into absolute ethanol. 9. 20 dips in first absolute ethanol 10. 1 minute in absolute ethanol 11. Clear and mount. Any suggestions would be really appreciated, Tim Wheelock Harvard Brain Bank Mclean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From latecor <@t> adinet.com.uy Wed Nov 26 17:28:02 2014 From: latecor <@t> adinet.com.uy (Carlos Defeo) Date: Wed Nov 26 17:27:56 2014 Subject: [Histonet] Congo Red problems In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC9773C9601@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC9773C9601@PHSX10MB11.partners.org> Message-ID: <201411262128020715.000E90C2@adinet.com.uy> Why don´t you use the classic Highman´s Congo red hidroalcoholic solution? (0,5% in 80% ethanol). Best luck, Carlos. *********** REPLY SEPARATOR *********** On 26/11/2014 at 20:48 Wheelock, Timothy R. wrote: >Hi All: > >Lately, I have been having problems with my Congo Red staining too lightly. >The Bielschowsky silver stain shows lots of amyloid in the cerebral blood >vessels and the cores of the senile plaques. >However, the Congo Red stain is pretty faint, although you can still see >it. >I have just bought a new bottle of Congo red from Sigma, in case it was >the particular batch of dye. >My protocol, which I have always used, is as follows: > >1. Deparaffinize sections and bring down to 95% ethanol >2. Stain in 1% Congo Red solution for 10 minutes. >3. Differentiate in Potassium Hydroxide solution; 3 dips >4. Rinse in 4-5 changes of tap water. >5. Counter-stain in Harris Hematoxylin for 1 minute. >6. Differentiate in Acid Alcohol; 10 dips or so. >7. Blue Hematoxylin in running tap water for 10 minutes. >8. 95% ethanol for only 3 dips, then right into absolute ethanol. >9. 20 dips in first absolute ethanol >10. 1 minute in absolute ethanol >11. Clear and mount. > >Any suggestions would be really appreciated, > >Tim Wheelock >Harvard Brain Bank >Mclean Hospital >Belmont, MA > > >The information in this e-mail is intended only for the person to whom it >is >addressed. If you believe this e-mail was sent to you in error and the >e-mail >contains patient information, please contact the Partners Compliance >HelpLine at >http://www.partners.org/complianceline . If the e-mail was sent to you in >error >but does not contain patient information, please contact the sender and >properly >dispose of the e-mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Tue Nov 25 02:13:09 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Wed Nov 26 22:15:52 2014 Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek In-Reply-To: References: Message-ID: <00dd01d00887$a8161110$f8423330$@rowaihi@alborglaboratories.com> Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Tuesday, November 25, 2014 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From richard.vernon <@t> thermofisher.com Fri Nov 28 03:47:42 2014 From: richard.vernon <@t> thermofisher.com (Vernon, Richard I.) Date: Fri Nov 28 03:47:54 2014 Subject: [Histonet] Re: Reference to microtome micrometer thickness In-Reply-To: <20141128094237.B3034E4C447@usmx01.thermofisher.com> References: <20141128094237.B3034E4C447@usmx01.thermofisher.com> Message-ID: <8411B0BF0CE50F4EA179AB3A06DB5C03560F4F751C@UKHIG-MXVS01.emea.thermo.com> Hi Maxim, Does measuring section thickness on slide scanners get affected by the surface imperfections on the section? It has been indicated to me that surface imperfections certainly hinder scanning speed but what about the actual thickness measurements? Kind regards Richard Vernon Strategic Product Manager - Sectioning Products Anatomical Pathology Division Thermo Fisher Scientific Tudor Road, Manor Park, Runcorn, Cheshire, WA7 1TA, UK Tel:+44 (0) 1928 534122 | Mobile:+44 (0) 7825 119070 richard.vernon@thermofisher.com | http//:www.thermoscientific.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 28 November 2014 09:43 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 132, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Reference to microtome micrometer thickness (Barry Rittman) 2. Congo Red problems (Wheelock, Timothy R.) 3. Re: Congo Red problems (Carlos Defeo) 4. RE: Formalin smell in the last paraffin station in vip 6 tissue tek (Jamal) ---------------------------------------------------------------------- Message: 1 Date: Wed, 26 Nov 2014 12:05:53 -0600 From: Barry Rittman Subject: Re: [Histonet] Reference to microtome micrometer thickness Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 It has been a while since I read articles regarding section thickness but I have some comments: 1. On Sat, Nov 22, 2014 at 8:37 PM, Maxim Peshkov wrote: > Reuel, > It is right, that there are many external reasons for a thickness of > paraffin sections. > It is possible to measure the final stained sections with mathematical > methods by slide scanner. > I hope that the slide-scanner can be easily to calculate thickness of > section by their soft. > Each individually sections will have own specific thickness. > > Sincerely, > Maxim Peshkov, > Russia, > Taganrog. > mailto:Maxim_71@mail.ru > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 2 Date: Wed, 26 Nov 2014 20:48:00 +0000 From: "Wheelock, Timothy R." Subject: [Histonet] Congo Red problems To: "histonet@lists.utsouthwestern.edu" Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9773C9601@PHSX10MB11.partners.org> Content-Type: text/plain; charset="us-ascii" Hi All: Lately, I have been having problems with my Congo Red staining too lightly. The Bielschowsky silver stain shows lots of amyloid in the cerebral blood vessels and the cores of the senile plaques. However, the Congo Red stain is pretty faint, although you can still see it. I have just bought a new bottle of Congo red from Sigma, in case it was the particular batch of dye. My protocol, which I have always used, is as follows: 1. Deparaffinize sections and bring down to 95% ethanol 2. Stain in 1% Congo Red solution for 10 minutes. 3. Differentiate in Potassium Hydroxide solution; 3 dips 4. Rinse in 4-5 changes of tap water. 5. Counter-stain in Harris Hematoxylin for 1 minute. 6. Differentiate in Acid Alcohol; 10 dips or so. 7. Blue Hematoxylin in running tap water for 10 minutes. 8. 95% ethanol for only 3 dips, then right into absolute ethanol. 9. 20 dips in first absolute ethanol 10. 1 minute in absolute ethanol 11. Clear and mount. Any suggestions would be really appreciated, Tim Wheelock Harvard Brain Bank Mclean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 3 Date: Wed, 26 Nov 2014 21:28:02 -0200 From: "Carlos Defeo" Subject: Re: [Histonet] Congo Red problems To: "Histonet" Cc: "Histonet" Message-ID: <201411262128020715.000E90C2@adinet.com.uy> Content-Type: text/plain; charset="us-ascii" Why don?t you use the classic Highman?s Congo red hidroalcoholic solution? (0,5% in 80% ethanol). Best luck, Carlos. *********** REPLY SEPARATOR *********** On 26/11/2014 at 20:48 Wheelock, Timothy R. wrote: >Hi All: > >Lately, I have been having problems with my Congo Red staining too lightly. >The Bielschowsky silver stain shows lots of amyloid in the cerebral >blood vessels and the cores of the senile plaques. >However, the Congo Red stain is pretty faint, although you can still >see it. >I have just bought a new bottle of Congo red from Sigma, in case it was >the particular batch of dye. >My protocol, which I have always used, is as follows: > >1. Deparaffinize sections and bring down to 95% ethanol 2. Stain in 1% >Congo Red solution for 10 minutes. >3. Differentiate in Potassium Hydroxide solution; 3 dips 4. Rinse in >4-5 changes of tap water. >5. Counter-stain in Harris Hematoxylin for 1 minute. >6. Differentiate in Acid Alcohol; 10 dips or so. >7. Blue Hematoxylin in running tap water for 10 minutes. >8. 95% ethanol for only 3 dips, then right into absolute ethanol. >9. 20 dips in first absolute ethanol >10. 1 minute in absolute ethanol >11. Clear and mount. > >Any suggestions would be really appreciated, > >Tim Wheelock >Harvard Brain Bank >Mclean Hospital >Belmont, MA > > >The information in this e-mail is intended only for the person to whom >it is addressed. If you believe this e-mail was sent to you in error >and the e-mail contains patient information, please contact the >Partners Compliance HelpLine at http://www.partners.org/complianceline >. If the e-mail was sent to you in error but does not contain patient >information, please contact the sender and properly dispose of the >e-mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 25 Nov 2014 11:13:09 +0300 From: "Jamal" Subject: RE: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek To: "'Arun Jyothi S.P'" , Message-ID: <00dd01d00887$a8161110$f8423330$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="UTF-8" Hi If the paraffin are overheated it gives abnormal odor. Recheck the paraffin bath temperature and the paraffin melting point then make sure to adjust the bath temperature at 2 degrees above the melting point. Please update me. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Arun Jyothi S.P Sent: Tuesday, November 25, 2014 9:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin smell in the last paraffin station in vip 6 tissue tek Dear all After processing in vip 6 the last paraffin has a strong odour of formalin Have anybody experienced the same Any ideas why it is happening. Arun Kuwait _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 132, Issue 29 ***************************************** From barryrittman <@t> gmail.com Fri Nov 28 11:33:37 2014 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Fri Nov 28 11:33:46 2014 Subject: [Histonet] Re: Reference to microtome micrometer thickness In-Reply-To: <8411B0BF0CE50F4EA179AB3A06DB5C03560F4F751C@UKHIG-MXVS01.emea.thermo.com> References: <20141128094237.B3034E4C447@usmx01.thermofisher.com> <8411B0BF0CE50F4EA179AB3A06DB5C03560F4F751C@UKHIG-MXVS01.emea.thermo.com> Message-ID: This has to do with section thickness. Would ne nice is did no0t have several other topics un der the same heading. Hi had a senior moment earlier so now will try to complete my sentences before pressing the send key.. I am not sure that it is essential to know accurately the thickness of sections, but the consistency of the sections in a ribbon. The microtome setting is only an approximate guide as the section thickness depends on many factors including the tissue density and homogeneity, temperature of the block and room, speed and angle of cutting, time between individual sections, whether the block has been moistened with ice water between sections and finally the skill and mood of the operator. In limits between 10 and 1 microns the thinner the sections the greater the chance of uneven thickness between sections. I think that Steedman's book on section cutting explained that an individual section is cleaved from the block, and like cheese slicing with a regular knife (sorry I know that you are all recovering from food overload) the thinner the slice the tendency is for less consistency between individual slices. If you cut a ribbon of sections then the consistency of individual sections once the slide is stained should be fairly obvious and easy to measure using image analysis of density and/or color. It is possible with experience to determine the difference between 4 and 7 micron sections but I do not believe the difference of 1 micron between individual sections can be easily determined. There are a couple of ways to be very accurate but I am not sure the effort is really worth it. 1. Use a homogenous block of protein such that is radiolabeled, embed and section with your block of tissue and use a counter on sections to determine how much activity and therefore thickness of individual sections. 2. Use a block of protein stained with solvent resistant dye and measure using image analysis. As you know the concentration of the dye you can determine how much is in an individual section. Many Procion dyes (chloro s triazines) bind tenaciously to proteins and are resistant to most chemical used in the histology lab. 3. Use labeled or stained micro-spheres of known diameter in the block and determine how many profiles you have when focusing through a section. 4. Use built in markers such as red blood cell in small vessels, there should be enough there to determine approximate thickness. While this an interesting problem I would suggest that the easiest approach, unless you have no social life, is to rely on the skill of the microtomist. Barry On Fri, Nov 28, 2014 at 3:47 AM, Vernon, Richard I. < richard.vernon@thermofisher.com> wrote: > Hi Maxim, > > Does measuring section thickness on slide scanners get affected by the > surface imperfections on the section? > > It has been indicated to me that surface imperfections certainly hinder > scanning speed but what about the actual thickness measurements? > > > Kind regards > > Richard Vernon > Strategic Product Manager - Sectioning Products > Anatomical Pathology Division > > Thermo Fisher Scientific > Tudor Road, Manor Park, Runcorn, Cheshire, WA7 1TA, UK > Tel:+44 (0) 1928 534122 | Mobile:+44 (0) 7825 119070 > richard.vernon@thermofisher.com | http//:www.thermoscientific.com > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: 28 November 2014 09:43 > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 132, Issue 29 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than > "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Reference to microtome micrometer thickness (Barry Rittman) > 2. Congo Red problems (Wheelock, Timothy R.) > 3. Re: Congo Red problems (Carlos Defeo) > 4. RE: Formalin smell in the last paraffin station in vip 6 > tissue tek (Jamal) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 26 Nov 2014 12:05:53 -0600 > From: Barry Rittman > Subject: Re: [Histonet] Reference to microtome micrometer thickness > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > CA+0tsF3q_YxA2y2bjRgxeF8tiX_FAw6Miv-BnaYME75X7-hULg@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > It has been a while since I read articles regarding section thickness but > I have some comments: > 1. > > On Sat, Nov 22, 2014 at 8:37 PM, Maxim Peshkov wrote: > > > Reuel, > > It is right, that there are many external reasons for a thickness of > > paraffin sections. > > It is possible to measure the final stained sections with mathematical > > methods by slide scanner. > > I hope that the slide-scanner can be easily to calculate thickness of > > section by their soft. > > Each individually sections will have own specific thickness. > > > > Sincerely, > > Maxim Peshkov, > > Russia, > > Taganrog. > > mailto:Maxim_71@mail.ru > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > > > > > > From mitchell <@t> wanpost.com Sun Nov 30 05:54:33 2014 From: mitchell <@t> wanpost.com (Mitchell Wan Home) Date: Sun Nov 30 05:55:50 2014 Subject: [Histonet] Looking for a second hand Cryostat plus Message-ID: Hello Histo friends, Looking for a second hand Cryostat. Located in Brisbane,? Australia.? I can organise pickup around Australia. Still looking for other Histo equipment.? Eg. Stainers, Processors, Embedding centres, and microscopes. Thank you for the assistance throughout the year. Regards? Mitchell 0418 745 750 mitchell@wanpost.com From marjoh3 <@t> telus.net Sun Nov 30 09:44:32 2014 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sun Nov 30 09:44:39 2014 Subject: [Histonet] Reichert Jung Autocut Microtome & LKB Knifemaker For Glycolmethacrylate Blocks For Sale Message-ID: Hi Histonetters, After completing a skin/tumor project by Glycolmethacrylate (GMA), I have a Reichert Jung 1150 Autocut microtome, LKB 2078 Histo Knifemaker and 2 boxes of 1" glass strips for sale. I would like to sell all of these items together. Marilyn Johnson Edmonton, AB. Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet