From ibernard <@t> uab.edu Thu May 1 05:15:57 2014 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Thu May 1 05:16:00 2014 Subject: [Histonet] RE: Special stain control validation- CAP compliance In-Reply-To: References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> <3566D9E34287BE4B95372179009446A01CFDB11F@EXCHANGE.fmhnt.fmh.org> <77DD817201982748BC67D7960F2F76AF0B2CD1@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Documentation is a key aspect of lab work. It is almost like doing the practice without procedures. Its shows history, continuity and evidence that the work was methodical, approved and sound. Thus it provides evidence based substantiation. Put all you do in practice in written language and you will be sound with this requirement. My take IB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy McLaughlin Sent: Wednesday, April 30, 2014 7:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stain control validation- CAP compliance Hello, We validate our control blocks prior to patient use. We label the slide with the stain, date, and results. We keep the slides in a file for reference. Our recent CAP inspection cited us on this because it was not documented on paper. I am considering challenging this, as I feel what we're doing meets the intent of the checklist question. What are your opinions? Thanks, Stacy **NEW** 07/29/2013 ANP.21460 Special Stain Controls Phase I Validated tissue controls are required for each special stain. NOTE: Positive tissue controls assess the performance of the special stain. Special stains are performed on sections of control tissue known to contain components specific to each special stain. Validation of tissue used as a positive control must be performed and documented before being used with clinical specimens. Evidence of Compliance: ? Written results of special stain control tissue validation [Cooley-Dickinson.org] From histotalk <@t> yahoo.com Thu May 1 10:10:56 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu May 1 10:11:00 2014 Subject: [Histonet] Daily Histo Tip Message-ID: <1398957056.42765.YahooMailNeo@web141103.mail.bf1.yahoo.com> Hello Everyone - Just a quick note to invite you to a NEW Histology Quickie! It premiers today at Daily HistoTIP. A special thank you to Wanda Simons, Lamar & Wanda Jones, Jack Ratliff, Vinnie Della Speranza, Shirley Powell, Mike Bourguise, Skip Brown and Bill DeSalvo for their inspiration in this endeavor! Yours, Dave Daily HistoTIP Daily HistoTIP View on www.DailyHistoTip.com Preview by Yahoo From rmire <@t> cvpath.org Thu May 1 11:39:20 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Thu May 1 11:39:42 2014 Subject: [Histonet] tissue processor regulations Message-ID: Hello Histonet, I recently started work in a research lab after working in a clinical setting for 20 years. The lab that I was at had all the tissue processors in a separate negative pressure room. The lab that I work at now has the processors out in the main work area. I noticed the fumes instantly and now several of the employees have complained of problems with the fumes. I want to move all the processors away from the cutting areas into a separate room. Any suggestions or comments that I could add to my justification would be helpful. Thanks Ronda Mire Laboratory Manager CVPath Institute Gaithersburg, MD From histogirl4 <@t> gmail.com Thu May 1 12:14:37 2014 From: histogirl4 <@t> gmail.com (histogirl4@gmail.com) Date: Thu May 1 12:26:02 2014 Subject: [Histonet] (no subject) Message-ID: <536283a5.8583320a.2640.ffffef55@mx.google.com> Job Opportunity: Pathology Associates of Greenville is seeking a full-time Histotechnologist/Histotechnician, to join practice. We are a free-standing anatomic pathology laboratory with hospital affiliations, staffed by five pathologists and two full-time Histotechnologists/Histotechnicians. Our laboratory is fully automated, We perform routine surgical pathology, few non-gyn cytology, special stains (Histochemisty) and Immunhistochemistry (IHC). Requirements for the successful applicant-- Strong Work Ethics_ Excellent Interpersonal Skills and willinf to assit in Frzen Sections and maintaining policies and procedures of The Histology Laboratory, Canidates are preferably ASCP Certified/Eligible, Intereseted applicants may fax their resume to 903-454-1716. E-mail addreass: ebrmo@yahoo.com Sent from Windows Mail From sforeman <@t> labpath.com Thu May 1 13:52:59 2014 From: sforeman <@t> labpath.com (Susan Foreman) Date: Thu May 1 13:54:38 2014 Subject: [Histonet] Ventana Benchmark Ultra and FITC antibody stains Message-ID: <00a001cf656e$92af8ca0$b80ea5e0$@com> Is anyone running FITC stains on the Ventana Benchmark Ultra? Are you able to utilize Prep Kits or do the antibodies have to purchased directly from Ventana. I appreciate your feedback. Many Thanks, Susan From akbitting <@t> geisinger.edu Thu May 1 14:46:30 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu May 1 14:46:44 2014 Subject: [Histonet] Ventana Benchmark Ultra and FITC antibody stains In-Reply-To: <00a001cf656e$92af8ca0$b80ea5e0$@com> References: <00a001cf656e$92af8ca0$b80ea5e0$@com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F679BE9167@GHSEXMBX1W8K1V.geisinger.edu> FITCs have to be purchased directly from Ventana. I've been trying to figure out how to "trick" their system because I have third partys that I'd like to try out. They don't offer an option to choose a Prep kit in their FITC template. When I called in to ask why they don't allow it they told me they didn't have that much interest from people who wanted to do that. Good luck, Angie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Foreman Sent: Thursday, May 01, 2014 2:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Benchmark Ultra and FITC antibody stains Is anyone running FITC stains on the Ventana Benchmark Ultra? Are you able to utilize Prep Kits or do the antibodies have to purchased directly from Ventana. I appreciate your feedback. Many Thanks, Susan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From DSiena <@t> statlab.com Thu May 1 16:15:18 2014 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu May 1 16:15:23 2014 Subject: [Histonet] Toluidine Blue stain for Mohs Message-ID: Hi All, I am in need of a Toluidine Blue stain for Moh's Lab, would anyone be willing to share with me? It is for a friend. In advance, thank you very much. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com From madeathridge <@t> pastnashville.com Fri May 2 10:56:02 2014 From: madeathridge <@t> pastnashville.com (Mary Ann Deathridge) Date: Fri May 2 10:56:48 2014 Subject: [Histonet] Histotech search Message-ID: <61753084$1e91c208$7ebfe8cb$@com> Histotechnician (Nashville, TN)) Seeking Experienced Histotech for full time position in a busy laboratory. Benefits include: PTO accural, Healthcare, 401k Hours: 4:00am -12:30pm Please send resume with inquiry. 615-298-4100, Fax: 615-298-4141 From jkaczmarek <@t> naplespathology.com Fri May 2 13:15:11 2014 From: jkaczmarek <@t> naplespathology.com (Joan Kaczmarek) Date: Fri May 2 13:15:20 2014 Subject: [Histonet] Stains for CD83 and DC-LAMP Message-ID: <1FDEF09842F10B449AE533BA7994431E9D50@SBS2011.NaplesPA.local> Does anyone do these stains, CD83 and DC-LAMP (CD208) or know a reliable source to obtain them? I would prefer to find a reference lab that would do these stains for our Lab. Thanks. Joan Kaczmarek, HTL (ASCP) Asst. Laboratory Manager, Naples Pathology Associates 4351 Tamiami Trail N, Naples, FL 34103 phone 239-263-1777 fax 239-263-6983 From histotalk <@t> yahoo.com Sat May 3 10:53:08 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Sat May 3 10:56:05 2014 Subject: [Histonet] No more emails? Message-ID: <1399132388.84485.YahooMailNeo@web141102.mail.bf1.yahoo.com> Apparently there is a problem somewhere along the way. I stopped receiving HistoNet emails yesterday morning. Actually, it's very sporadic. Has anyone else had this problem?? Yours, Dave From jrsmallwood <@t> bell.net Sat May 3 11:10:08 2014 From: jrsmallwood <@t> bell.net (John Smallwood) Date: Sat May 3 11:10:20 2014 Subject: [Histonet] Basement Lab Message-ID: Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? Than you, John Smallwood, MLT. London, Ont. Can. From rjbuesa <@t> yahoo.com Sat May 3 11:35:30 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 3 11:38:20 2014 Subject: [Histonet] Basement Lab In-Reply-To: References: Message-ID: <1399134930.46899.YahooMailNeo@web120405.mail.ne1.yahoo.com> You have to talk with the fire marshal of your town with the plans in hand and explain him/her what products you use and what type of ventilation and safety characteristics?the lab will have. If s/he agrees with the plans there is little you can do about it, especially considering that historically the basement has been the site for many a histolab. But is the fire marshal disagrees you can take his opinion to your manager. Ren? J. ? On Saturday, May 3, 2014 12:10 PM, John Smallwood wrote: ? Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? Than you, John Smallwood, MLT. London, Ont. Can. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Sat May 3 14:50:46 2014 From: suetp918 <@t> comcast.net (Sue) Date: Sat May 3 14:52:31 2014 Subject: [Histonet] Basement Lab In-Reply-To: References: Message-ID: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Sat May 3 18:51:38 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat May 3 18:51:44 2014 Subject: [Histonet] Basement Lab In-Reply-To: References: Message-ID: <79DD38699F14435AAC948C34C959ED0C@HP2010> You need to meet with the architect, and engineer, and the fire marshal. And they need to be knowledgeable about the chemicals, etc. that will be stored/used in the lab area, and any rules/regulations in your country/area that relate to building labs. The laws have changed in many locations, so that they want the fire department to have easy access to the lab, in case of a fire. Think of all the flammables we have - how many gallons of alcohol, xylene, acetone, etc. Most of us are already in the basement, so we are grandfathered in. But anyone building a new lab, or an addition to a lab, will be under the new rules. Most of the time, they want the lab built on the 1st or 2nd floor, so the fire hoses don't have to be dragged up or down stairs or ladders. And they want the lab on a outside wall, again, so the fire fighters don't have to be hauling hoses into the middle of a building. And the outside wall can't be near where people will be walking by, in case there is a chemical blow out of the wall. That being said, that doesn't mean that labs can't be built in the basement or, say, on the 5th floor. There are just a lot of additional conditions - walls, floors and ceiling that are thicker and can resist being burned through for longer periods of time, denser doors for fire resistance, automatic sprinkler systems that are closer together,etc. So again, you need to meet with people who are knowledgeable about the type and amount of flammable chemicals that are going to be used in that area, and the laws that govern safety for building labs. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: John Smallwood Sent: Saturday, May 03, 2014 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Basement Lab Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? Than you, John Smallwood, MLT. London, Ont. Can. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Mon May 5 02:19:05 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Mon May 5 02:19:13 2014 Subject: [Histonet] Basement Lab In-Reply-To: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> References: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net> Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon May 5 08:22:06 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 5 08:22:10 2014 Subject: [Histonet] Basement Lab In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net> References: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <1399296126.79225.YahooMailNeo@web120404.mail.ne1.yahoo.com> Histology is a very OLD "art" and by the end of the XIX and up to the middle of the XX century the "rule" was to do histology near the morgue?that was almost always in the hospital basement. Additionally also since the start the laboratory activity less "respected" was that of histotech, always considered "inferior" to the medical lab tech. Histology was practiced from the start by "people" trained "to do histology" and the histology techs?were recruited from janitors, secretaries, or pathologists' family members. It was just a "training" in how to process, section and stain, like a "flesh and bone" instrument. This is a very long trend just now crumbling down because of CAP requirements and even perhaps as a sorts of "human rights" issue BUT there is always something remaining and nobody is surprised if the histology lab is?relegated to the basement which is a non-premium real estate within the hospital. That is why! Oh those old days that in this case are not "good old days"! Ren? J.? On Monday, May 5, 2014 3:19 AM, "Susan.Walzer@HCAHealthcare.com" wrote: Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > >? Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cindy38017 <@t> yahoo.com Mon May 5 08:48:41 2014 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Mon May 5 08:54:52 2014 Subject: [Histonet] PRN position Message-ID: <1399297721.43534.YahooMailNeo@web163902.mail.gq1.yahoo.com> Busy dermpath lab in Cincinnati is in need of a prn tech to cover a 12 week maternity leave, starting the middle to end of July .This is a full time position, hours being 5:30 to 2:00, or 6:30 to 3:00. ASCP registered is preferred but will consider registry eligible. Tech?must be able to meet CLIA requirements for grossing. If interested, please?email me at cindy38017@yahoo.com. Thank You! From POWELL_SA <@t> mercer.edu Mon May 5 10:12:32 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon May 5 10:12:40 2014 Subject: [Histonet] Basement Lab In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net> References: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF778A621@MERCERMAIL.MercerU.local> Because we are explosive and flammable? ................ I know it is terrible, they need us but want to hide us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Monday, May 05, 2014 3:19 AM To: suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon May 5 10:15:07 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon May 5 10:17:00 2014 Subject: [Histonet] Basement Lab In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25BF778A621@MERCERMAIL.MercerU.local> References: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net> <9BF995BC0E47744E9673A41486E24EE25BF778A621@MERCERMAIL.MercerU.local> Message-ID: <3B2CD438E1628A41BD687E98B963B7811FCF52FF@EMBX-CHAM2.cdc.gov> We have a beautiful window view....I would insist on windows! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, May 05, 2014 11:13 AM To: Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Because we are explosive and flammable? ................ I know it is terrible, they need us but want to hide us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Monday, May 05, 2014 3:19 AM To: suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon May 5 10:22:21 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon May 5 10:23:17 2014 Subject: [Histonet] Basement Lab In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FCF52FF@EMBX-CHAM2.cdc.gov> References: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net> <9BF995BC0E47744E9673A41486E24EE25BF778A621@MERCERMAIL.MercerU.local> <3B2CD438E1628A41BD687E98B963B7811FCF52FF@EMBX-CHAM2.cdc.gov> Message-ID: <25A4DE08332B19499904459F00AAACB719DE6DEDA2@EVS1.archildrens.org> We too have windows!! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Monday, May 05, 2014 10:15 AM To: Shirley A. Powell; Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab We have a beautiful window view....I would insist on windows! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, May 05, 2014 11:13 AM To: Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Because we are explosive and flammable? ................ I know it is terrible, they need us but want to hide us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Monday, May 05, 2014 3:19 AM To: suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From thisisann <@t> aol.com Mon May 5 10:30:34 2014 From: thisisann <@t> aol.com (Ann Specian) Date: Mon May 5 10:30:39 2014 Subject: [Histonet] Slide oven temperature Message-ID: <8D1368E1780B9BE-3DE0-1E0A7@webmail-d225.sysops.aol.com> Can someone tell me the range you set your slide drying oven at and what you base it on. thanks, Ann From jqb7 <@t> cdc.gov Mon May 5 10:31:06 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon May 5 10:31:21 2014 Subject: [Histonet] Basement Lab In-Reply-To: <25A4DE08332B19499904459F00AAACB719DE6DEDA2@EVS1.archildrens.org> References: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net> <9BF995BC0E47744E9673A41486E24EE25BF778A621@MERCERMAIL.MercerU.local> <3B2CD438E1628A41BD687E98B963B7811FCF52FF@EMBX-CHAM2.cdc.gov> <25A4DE08332B19499904459F00AAACB719DE6DEDA2@EVS1.archildrens.org> Message-ID: <3B2CD438E1628A41BD687E98B963B7811FCF533E@EMBX-CHAM2.cdc.gov> Quite the mood-lifter! -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Monday, May 05, 2014 11:22 AM To: Sanders, Jeanine (CDC/OID/NCEZID); Shirley A. Powell; Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab We too have windows!! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Monday, May 05, 2014 10:15 AM To: Shirley A. Powell; Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab We have a beautiful window view....I would insist on windows! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, May 05, 2014 11:13 AM To: Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Because we are explosive and flammable? ................ I know it is terrible, they need us but want to hide us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Monday, May 05, 2014 3:19 AM To: suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From GaleL <@t> unionhospital.org Mon May 5 11:38:17 2014 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Mon May 5 11:38:46 2014 Subject: [Histonet] specimen release Message-ID: We are looking into revising our policy/release form regarding the release of specimens to patients and I am interested in knowing what others are doing. Thanks, Gale Gale Limron HT,CT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From barbara.tibbs <@t> accuratediagnosticlabs.com Mon May 5 12:04:38 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Mon May 5 12:05:10 2014 Subject: [Histonet] Basement Lab In-Reply-To: <1399296126.79225.YahooMailNeo@web120404.mail.ne1.yahoo.com> References: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net>, <1399296126.79225.YahooMailNeo@web120404.mail.ne1.yahoo.com> Message-ID: <1399309442611.52585@accuratediagnosticlabs.com> This is very true. When I started back in the 70s, the lab was in the basement right down the hall from the morgue. The only other department down there with us was the receiving department. The only difference from other histology labs was that this was the lab of the NAACLS accredited Histotechnology hospital based program in which I was a student. All the techs were graduates of the program and were involved in teaching the two students. I consider myself lucky that I trained this way rather than depending on the good graces of older histotechs teaching me at their whim. But, to be fair, I learned a great deal from the "on-the-bench" trained techs through the years. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Monday, May 05, 2014 12:22 PM To: Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basement Lab Histology is a very OLD "art" and by the end of the XIX and up to the middle of the XX century the "rule" was to do histology near the morgue that was almost always in the hospital basement. Additionally also since the start the laboratory activity less "respected" was that of histotech, always considered "inferior" to the medical lab tech. Histology was practiced from the start by "people" trained "to do histology" and the histology techs were recruited from janitors, secretaries, or pathologists' family members. It was just a "training" in how to process, section and stain, like a "flesh and bone" instrument. This is a very long trend just now crumbling down because of CAP requirements and even perhaps as a sorts of "human rights" issue BUT there is always something remaining and nobody is surprised if the histology lab is relegated to the basement which is a non-premium real estate within the hospital. That is why! Oh those old days that in this case are not "good old days"! Ren? J. On Monday, May 5, 2014 3:19 AM, "Susan.Walzer@HCAHealthcare.com" wrote: Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > >? Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Mon May 5 12:56:15 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon May 5 12:56:29 2014 Subject: [Histonet] ISH Message-ID: We are having sporadic issues with kappa and lambda ISH on our bone marrows. What effect does EDTA have on ISH? The same as decal? Thanks for your help! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From CIngles <@t> uwhealth.org Mon May 5 13:43:08 2014 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon May 5 13:43:12 2014 Subject: [Histonet] Basement Lab In-Reply-To: <1399309442611.52585@accuratediagnosticlabs.com> References: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net>, <1399296126.79225.YahooMailNeo@web120404.mail.ne1.yahoo.com>, <1399309442611.52585@accuratediagnosticlabs.com> Message-ID: Our main Histology lab at UW Madison Hospital is in the basement of the VA (They're) attached. We have to go UP a floor to get to the morgue. Although why they put the morgue on the main level is beyond me... Oh, wait. government administration... I am at one of the outlying clinics and at least we can walk by windows once in a while. :) But for some reason we do get paid more than the med techs here. Knock me over with a feather. Claire ________________________________________ From carl.hobbs <@t> kcl.ac.uk Mon May 5 13:51:37 2014 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Mon May 5 13:51:44 2014 Subject: [Histonet] Submitting images Message-ID: <1399315891515.65961@kcl.ac.uk> If possible, can an "edit" facility be included? Sometimes I do a typo error.... Mea culpa-ly. Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From lblazek <@t> digestivespecialists.com Mon May 5 14:14:16 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon May 5 14:14:21 2014 Subject: [Histonet] Basement Lab In-Reply-To: References: <1D1FE9C0-6EDC-4214-9523-290C39ECEDE7@comcast.net> <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net>, <1399296126.79225.YahooMailNeo@web120404.mail.ne1.yahoo.com>, <1399309442611.52585@accuratediagnosticlabs.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3916856B52ED@IBMB7Exchange.digestivespecialists.com> The life of a basement lab rat isn't all bad! We don't hear thunder storms. We are probably be safe from tornados, hurricanes and will have lots of company if one does come by. Usually no one wants to go to the basement. There are no windows so there isn't any of the outside world distraction. If it's a beautiful day you don't see it so you don't long to be out there. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, May 05, 2014 2:43 PM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Our main Histology lab at UW Madison Hospital is in the basement of the VA (They're) attached. We have to go UP a floor to get to the morgue. Although why they put the morgue on the main level is beyond me... Oh, wait. government administration... I am at one of the outlying clinics and at least we can walk by windows once in a while. :) But for some reason we do get paid more than the med techs here. Knock me over with a feather. Claire ________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader <@t> mcgill.ca Mon May 5 14:24:34 2014 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Mon May 5 14:25:17 2014 Subject: [Histonet] Whole mouse fixation Message-ID: <8C36045F0065CE48906E684F15FD4CB6321566CD@EXMBX2010-6.campus.MCGILL.CA> Good afternoon Histonetters, I have a client who wants us to process,embed and cut saggital sections of a whole mouse which is 8-9 months old. I have a pretty good idea of what needs to be done but I would like some feedback from those who have more experience than I do. Fixation time, and processing times. Thanks in advance. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From Richard.Cartun <@t> hhchealth.org Mon May 5 14:56:30 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon May 5 14:57:38 2014 Subject: [Histonet] RE: specimen release In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E022B5320@HHCEXCHMB03.hhcsystem.org> In the state of Connecticut we can only release human tissue to a funeral home. Other than a hospital or a laboratory, a funeral home is the only company licensed to handle human tissue. The patient then deals with the funeral home. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Monday, May 05, 2014 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen release We are looking into revising our policy/release form regarding the release of specimens to patients and I am interested in knowing what others are doing. Thanks, Gale Gale Limron HT,CT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From trathborne <@t> somerset-healthcare.com Mon May 5 15:34:34 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon May 5 15:35:46 2014 Subject: [Histonet] RE: specimen release In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E022B5320@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E022B5320@HHCEXCHMB03.hhcsystem.org> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95DE8CA@SMCMAIL01.somerset-healthcare.com> You may want to consider the CAP checklist which has recently been revised. **REVISED** 04/21/2014 ANP.24200 Biohazard Waste Disposal Phase II Infectious tissues and other potentially contaminated materials are disposed of with minimum danger to professional, technical, and custodial personnel, and to recipients. NOTE: Waste disposal must be in accord with all regulations and disposed of with minimum danger. Specimens returned to professional, technical, and custodial personnel patients (e.g. prostheses, gallstones) or otherwise released to others (e.g. pacemaker or prosthesis to manufacturer) must be disinfected before release. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Monday, May 05, 2014 3:57 PM To: Gale Limron; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specimen release In the state of Connecticut we can only release human tissue to a funeral home. Other than a hospital or a laboratory, a funeral home is the only company licensed to handle human tissue. The patient then deals with the funeral home. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Monday, May 05, 2014 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen release We are looking into revising our policy/release form regarding the release of specimens to patients and I am interested in knowing what others are doing. Thanks, Gale Gale Limron HT,CT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Mon May 5 15:44:41 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon May 5 15:45:46 2014 Subject: [Histonet] RE: specimen release In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95DE8CA@SMCMAIL01.somerset-healthcare.com> References: <9215BD4B0BA1B44D962A71C758B68D2E022B5320@HHCEXCHMB03.hhcsystem.org> <3AD061FE740D464FAC7BF6B5CFB75707A95DE8CA@SMCMAIL01.somerset-healthcare.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95DE95D@SMCMAIL01.somerset-healthcare.com> Correction! My first response did not accept the copy/paste strikethroughs. NOTE: Waste disposal must be in accord with all regulations and disposed of with minimum danger. Specimens returned to professional, technical, and custodial personnel patients (e.g. prostheses, gallstones) or otherwise released to others (e.g. pacemaker or prosthesis to manufacturer) must be disinfected before release. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Monday, May 05, 2014 4:35 PM To: 'Cartun, Richard'; Gale Limron; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specimen release You may want to consider the CAP checklist which has recently been revised. **REVISED** 04/21/2014 ANP.24200 Biohazard Waste Disposal Phase II Infectious tissues and other potentially contaminated materials are disposed of with minimum danger to professional, technical, and custodial personnel, and to recipients. NOTE: Waste disposal must be in accord with all regulations and disposed of with minimum danger. Specimens returned to professional, technical, and custodial personnel patients (e.g. prostheses, gallstones) or otherwise released to others (e.g. pacemaker or prosthesis to manufacturer) must be disinfected before release. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Monday, May 05, 2014 3:57 PM To: Gale Limron; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specimen release In the state of Connecticut we can only release human tissue to a funeral home. Other than a hospital or a laboratory, a funeral home is the only company licensed to handle human tissue. The patient then deals with the funeral home. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gale Limron Sent: Monday, May 05, 2014 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen release We are looking into revising our policy/release form regarding the release of specimens to patients and I am interested in knowing what others are doing. Thanks, Gale Gale Limron HT,CT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Mon May 5 15:52:21 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon May 5 15:52:25 2014 Subject: [Histonet] Re: specimen release Message-ID: Gale Limron HT,CT (ASCP), Histology Supervisor, Union Hospital, Dover, Ohio 44622 asks: >>We are looking into revising our policy/release form regarding the release of specimens to patients and I am interested in knowing what others are doing.<< This practice (gallstones, tonsils, etc.) has been pretty well stopped by the regulatory agencies, JCAHO I think. To no one's regret. There are some special problems: amputated legs and breasts for burial, fetuses, placentas for (uh, never mind) - and all of these are worth developing policies for. Off-topic, Gale, but congratulations on qualifying both as a histotechnologist and a cytotechnologist. This is going to become a necessity in small hospitals in the very near future. Bob Richmond Samurai Pathologist Maryville TN From joelleweaver <@t> hotmail.com Mon May 5 18:30:21 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon May 5 18:30:27 2014 Subject: [Histonet] Re: specimen release In-Reply-To: References: Message-ID: I remember when I lived in Ohio there was statute regarding release of POC to parents who were permitted to have it returned to them for mass & burial as a formed fetus. The legislature extended the burial right to earlier gestation, and for the most part it was truly not to the point of recognition as a fetus on gross. I don't remember the gestational age, but it was early. We had to package it in a special protected manner, transfer to 70% ethanol as I remember, and they signed some sort of release or consent. The actual transfer to them was done by pastoral care. On other rare occasions, patients or their families have wanted other tissues. It went kind of the same way ( after sign out & 2 week period), with consent, and agreement to dispose in accordance with local regulations, except for no pastoral care. My suspicion is this may be something that varies by state statute. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 5 May 2014 16:52:21 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: specimen release > > Gale Limron HT,CT (ASCP), Histology Supervisor, Union Hospital, Dover, Ohio > 44622 asks: > > >>We are looking into revising our policy/release form regarding the > release of specimens to patients and I am interested in knowing what others > are doing.<< > > This practice (gallstones, tonsils, etc.) has been pretty well stopped by > the regulatory agencies, JCAHO I think. To no one's regret. > > There are some special problems: amputated legs and breasts for burial, > fetuses, placentas for (uh, never mind) - and all of these are worth > developing policies for. > > Off-topic, Gale, but congratulations on qualifying both as a > histotechnologist and a cytotechnologist. This is going to become a > necessity in small hospitals in the very near future. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Tue May 6 09:18:01 2014 From: JWatson <@t> gnf.org (James Watson) Date: Tue May 6 09:19:26 2014 Subject: [Histonet] Basement Lab In-Reply-To: <79DD38699F14435AAC948C34C959ED0C@HP2010> References: <79DD38699F14435AAC948C34C959ED0C@HP2010> Message-ID: Our histology lab is in the basement here, but due to fire code in S.D. Ca. we are limited on the amount of flammables in the basement if the room is not explosion proof. So, our processor and flammable storage is on the first floor. Very LEAN. I did bring some of the outside into the lab, the hanging plants are doing quite well, so they make up for not seeing the light of day somewhat. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Saturday, May 03, 2014 4:52 PM To: John Smallwood; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Basement Lab You need to meet with the architect, and engineer, and the fire marshal. And they need to be knowledgeable about the chemicals, etc. that will be stored/used in the lab area, and any rules/regulations in your country/area that relate to building labs. The laws have changed in many locations, so that they want the fire department to have easy access to the lab, in case of a fire. Think of all the flammables we have - how many gallons of alcohol, xylene, acetone, etc. Most of us are already in the basement, so we are grandfathered in. But anyone building a new lab, or an addition to a lab, will be under the new rules. Most of the time, they want the lab built on the 1st or 2nd floor, so the fire hoses don't have to be dragged up or down stairs or ladders. And they want the lab on a outside wall, again, so the fire fighters don't have to be hauling hoses into the middle of a building. And the outside wall can't be near where people will be walking by, in case there is a chemical blow out of the wall. That being said, that doesn't mean that labs can't be built in the basement or, say, on the 5th floor. There are just a lot of additional conditions - walls, floors and ceiling that are thicker and can resist being burned through for longer periods of time, denser doors for fire resistance, automatic sprinkler systems that are closer together,etc. So again, you need to meet with people who are knowledgeable about the type and amount of flammable chemicals that are going to be used in that area, and the laws that govern safety for building labs. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: John Smallwood Sent: Saturday, May 03, 2014 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Basement Lab Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? Than you, John Smallwood, MLT. London, Ont. Can. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dosiagwu <@t> unilag.edu.ng Tue May 6 10:57:17 2014 From: dosiagwu <@t> unilag.edu.ng (DAVID OSIAGWU) Date: Tue May 6 10:29:18 2014 Subject: [Histonet] Re: Histonet Digest, Vol 126, Issue 5 In-Reply-To: <20140505164549.0F0E7983FC@mailgw.unilag.edu.ng> Message-ID: <722853951.6237286.1399391837285.JavaMail.root@unilag.edu.ng> I don't think it?s a good idea having the lab in a basement, no matter the design, think of all the flammable chemicals you will need to store. What happens to spills and fumes? i hope they will have enough good ventilation? Daniel ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, May 5, 2014 5:45:49 PM Subject: Histonet Digest, Vol 126, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Basement Lab (Susan.Walzer@HCAHealthcare.com) 2. Re: Basement Lab (Rene J Buesa) 3. PRN position (cindy dewar) 4. RE: Basement Lab (Shirley A. Powell) 5. RE: Basement Lab (Sanders, Jeanine (CDC/OID/NCEZID)) 6. RE: Basement Lab (Horn, Hazel V) 7. Slide oven temperature (Ann Specian) 8. RE: Basement Lab (Sanders, Jeanine (CDC/OID/NCEZID)) 9. specimen release (Gale Limron) ---------------------------------------------------------------------- Message: 1 Date: Mon, 5 May 2014 02:19:05 -0500 From: Subject: RE: [Histonet] Basement Lab To: , Cc: histonet@lists.utsouthwestern.edu Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FB876899E@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 5 May 2014 06:22:06 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Basement Lab To: "Susan.Walzer@HCAHealthcare.com" , "suetp918@comcast.net" , "jrsmallwood@bell.net" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <1399296126.79225.YahooMailNeo@web120404.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Histology is a very OLD "art" and by the end of the XIX and up to the middle of the XX century the "rule" was to do histology near the morgue?that was almost always in the hospital basement. Additionally also since the start the laboratory activity less "respected" was that of histotech, always considered "inferior" to the medical lab tech. Histology was practiced from the start by "people" trained "to do histology" and the histology techs?were recruited from janitors, secretaries, or pathologists' family members. It was just a "training" in how to process, section and stain, like a "flesh and bone" instrument. This is a very long trend just now crumbling down because of CAP requirements and even perhaps as a sorts of "human rights" issue BUT there is always something remaining and nobody is surprised if the histology lab is?relegated to the basement which is a non-premium real estate within the hospital. That is why! Oh those old days that in this case are not "good old days"! Ren? J.? On Monday, May 5, 2014 3:19 AM, "Susan.Walzer@HCAHealthcare.com" wrote: Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > >? Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 5 May 2014 06:48:41 -0700 (PDT) From: cindy dewar Subject: [Histonet] PRN position To: "histonet@lists.utsouthwestern.edu" Message-ID: <1399297721.43534.YahooMailNeo@web163902.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Busy dermpath lab in Cincinnati is in need of a prn tech to cover a 12 week maternity leave, starting the middle to end of July .This is a full time position, hours being 5:30 to 2:00, or 6:30 to 3:00. ASCP registered is preferred but will consider registry eligible. Tech?must be able to meet CLIA requirements for grossing. If interested, please?email me at cindy38017@yahoo.com. Thank You! ------------------------------ Message: 4 Date: Mon, 5 May 2014 11:12:32 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] Basement Lab To: "Susan.Walzer@HCAHealthcare.com" , "suetp918@comcast.net" , "jrsmallwood@bell.net" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF778A621@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" Because we are explosive and flammable? ................ I know it is terrible, they need us but want to hide us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Monday, May 05, 2014 3:19 AM To: suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 5 May 2014 15:15:07 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" Subject: RE: [Histonet] Basement Lab To: "Shirley A. Powell" , "Susan.Walzer@HCAHealthcare.com" , "suetp918@comcast.net" , "jrsmallwood@bell.net" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <3B2CD438E1628A41BD687E98B963B7811FCF52FF@EMBX-CHAM2.cdc.gov> Content-Type: text/plain; charset="us-ascii" We have a beautiful window view....I would insist on windows! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, May 05, 2014 11:13 AM To: Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Because we are explosive and flammable? ................ I know it is terrible, they need us but want to hide us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Monday, May 05, 2014 3:19 AM To: suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 5 May 2014 10:22:21 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] Basement Lab To: "'Sanders, Jeanine (CDC/OID/NCEZID)'" , "Shirley A. Powell" , "Susan.Walzer@HCAHealthcare.com" , "suetp918@comcast.net" , "jrsmallwood@bell.net" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <25A4DE08332B19499904459F00AAACB719DE6DEDA2@EVS1.archildrens.org> Content-Type: text/plain; charset="us-ascii" We too have windows!! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Monday, May 05, 2014 10:15 AM To: Shirley A. Powell; Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab We have a beautiful window view....I would insist on windows! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, May 05, 2014 11:13 AM To: Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Because we are explosive and flammable? ................ I know it is terrible, they need us but want to hide us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Monday, May 05, 2014 3:19 AM To: suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 7 Date: Mon, 5 May 2014 11:30:34 -0400 (EDT) From: Ann Specian Subject: [Histonet] Slide oven temperature To: histonet@lists.utsouthwestern.edu Message-ID: <8D1368E1780B9BE-3DE0-1E0A7@webmail-d225.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Can someone tell me the range you set your slide drying oven at and what you base it on. thanks, Ann ------------------------------ Message: 8 Date: Mon, 5 May 2014 15:31:06 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" Subject: RE: [Histonet] Basement Lab To: "Horn, Hazel V" , "Shirley A. Powell" , "Susan.Walzer@HCAHealthcare.com" , "suetp918@comcast.net" , "jrsmallwood@bell.net" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <3B2CD438E1628A41BD687E98B963B7811FCF533E@EMBX-CHAM2.cdc.gov> Content-Type: text/plain; charset="us-ascii" Quite the mood-lifter! -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Monday, May 05, 2014 11:22 AM To: Sanders, Jeanine (CDC/OID/NCEZID); Shirley A. Powell; Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab We too have windows!! Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Monday, May 05, 2014 10:15 AM To: Shirley A. Powell; Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab We have a beautiful window view....I would insist on windows! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, May 05, 2014 11:13 AM To: Susan.Walzer@HCAHealthcare.com; suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Because we are explosive and flammable? ................ I know it is terrible, they need us but want to hide us. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Monday, May 05, 2014 3:19 AM To: suetp918@comcast.net; jrsmallwood@bell.net Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Basement Lab Why do they always want to put us in the basement? We have a lot of the hospitals explosives and flammables?? Still, as long as they have a good ventilation (you often need a roof or outside wall to do this) it might be ok. I hope they have good environmental engineers. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Saturday, May 03, 2014 3:51 PM To: John Smallwood Cc: Subject: Re: [Histonet] Basement Lab Not a good idea Sent from my iPhone > On May 3, 2014, at 12:10 PM, John Smallwood wrote: > > Our small Hospital with growth plans, is considering a new Laboratory in the basement of the planned tower. I consider this a less than desirable location. Spills , fumes, chemical allotments etc. What are Histonet members thoughts and ideas ?? > > Than you, > John Smallwood, MLT. > London, Ont. Can. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 9 Date: Mon, 5 May 2014 12:38:17 -0400 From: Gale Limron Subject: [Histonet] specimen release To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We are looking into revising our policy/release form regarding the release of specimens to patients and I am interested in knowing what others are doing. Thanks, Gale Gale Limron HT,CT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 126, Issue 5 **************************************** From millers <@t> emhs.org Tue May 6 10:56:50 2014 From: millers <@t> emhs.org (Miller, Suzie) Date: Tue May 6 10:57:01 2014 Subject: [Histonet] Pankeratin- Trouble with Mast Cell Staining Message-ID: Hello Everyone, If you have a Ventana Ultra and use (Ventana# 760-2595) Pankeratin AE1/AE3/PCK29, would you consider sharing your staining protocol? Our Pathologists are displeased with the amount of mast cell staining with our current protocol so we are looking to explore other protocols or suggestions. Thank you for your assistance, Suzie Suzie Miller, MLT ASCP Senior Histotech Mercy Health System of Maine Laboratory millers@emhs.org ------------------------------------------------------------------------------------------------------------------- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. From arguellod <@t> ihn.org Tue May 6 11:01:57 2014 From: arguellod <@t> ihn.org (Arguello, Daniel) Date: Tue May 6 11:02:55 2014 Subject: [Histonet] RE: Pankeratin- Trouble with Mast Cell Staining In-Reply-To: References: Message-ID: <46FD76128C5055478050297AEAC97E1771105938@EXCHANGE-1.corporate.lan> I'd appreciate a copy as well. Thanks Sincerely,? Daniel Arg?ello, BS, CT (ASCP)CM Anatomic Pathology Services Coordinator Inspira Medical Center Woodbury 509 North Broad Street Woodbury, NJ 08096 856-853-2030 Ext 2808 856-853-2183 Fax arguellod@ihn.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miller, Suzie Sent: Tuesday, May 06, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pankeratin- Trouble with Mast Cell Staining Hello Everyone, If you have a Ventana Ultra and use (Ventana# 760-2595) Pankeratin AE1/AE3/PCK29, would you consider sharing your staining protocol? Our Pathologists are displeased with the amount of mast cell staining with our current protocol so we are looking to explore other protocols or suggestions. Thank you for your assistance, Suzie Suzie Miller, MLT ASCP Senior Histotech Mercy Health System of Maine Laboratory millers@emhs.org ------------------------------------------------------------------------------------------------------------------- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Tue May 6 11:43:06 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue May 6 11:43:09 2014 Subject: [Histonet] RE: basement lab In-Reply-To: <20140506151948.153991E807B@trendmess-svr.holyredeemer.local> References: <20140506151948.153991E807B@trendmess-svr.holyredeemer.local> Message-ID: Basement Histology labs are not uncommon, as long as they meet all codes, not just flammables. For flammables, this means, not only storage, but also the volume present within the lab at anytime and that can vary by state. There is a defined volume per square footage of a contained fire area. This would include what is loaded onto processors, stainers, as well as what is stored. Generally, the max volume is reduced the higher the floor. Ventilation is not the only concern. Also, there will need to be 2 exits from each defined fire space, appropriate safety showers and eyewash stations which must be located 10 seconds away from possible sites of exposure. Eyewash and safety shower facilities should not be separated from the hazard site by a wall or partition that would cause an employee to go through a doorway. I've been through the construction/major remodel at all 3 labs where I've worked. 2 as supervisor. Unfortunately, you probably will have to become very familiar with OSHA and ANSI standards for safety and NFPA for codes concerning fire safety. Even builders/inspectors familiar with laboratory construction sometimes lack the knowledge to insure proper safety of a Histology Lab, its personnel and contents. Be vocal and adamant for safety, but be sure to back it up by providing the appropriate regulations. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Timothy.Morken <@t> ucsfmedctr.org Tue May 6 11:57:47 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue May 6 11:57:59 2014 Subject: [Histonet] RE: basement lab In-Reply-To: References: <20140506151948.153991E807B@trendmess-svr.holyredeemer.local> Message-ID: <761E2B5697F795489C8710BCC72141FF3677E81E@ex07.net.ucsf.edu> I worked in a basement lab for many years. And we had windows! The ground level outside the lab was higher but was sloped gently upwards so we got some sun. We had a concrete "bunker" for bulk flammables outside the lab, near the loading dock. We kept only daily-use amounts in the lab. Even so, in those days it was open-top staining runs (no automation!) so those not conditioned to the atmosphere of the typical histology lab would recoil at the alcohol and xylene fumes, not to mention the special stains aromas. We had a book shelf for the small chemicals and dyes. That's how we learned some chemicals should not be stored next to one another - One day red clouds of fumes ensued and the fire department paid a visit. And the rest of the lab got a partial day off.... So, yes, all the safety measures available should be used, along with knowledge... Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, May 06, 2014 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: basement lab Basement Histology labs are not uncommon, as long as they meet all codes, not just flammables. For flammables, this means, not only storage, but also the volume present within the lab at anytime and that can vary by state. There is a defined volume per square footage of a contained fire area. This would include what is loaded onto processors, stainers, as well as what is stored. Generally, the max volume is reduced the higher the floor. Ventilation is not the only concern. Also, there will need to be 2 exits from each defined fire space, appropriate safety showers and eyewash stations which must be located 10 seconds away from possible sites of exposure. Eyewash and safety shower facilities should not be separated from the hazard site by a wall or partition that would cause an employee to go through a doorway. I've been through the construction/major remodel at all 3 labs where I've worked. 2 as supervisor. Unfortunately, you probably will have to become very familiar with OSHA and ANSI standards for safety and NFPA for codes concerning fire safety. Even builders/inspectors familiar with laboratory construction sometimes lack the knowledge to insure proper safety of a Histology Lab, its personnel and contents. Be vocal and adamant for safety, but be sure to back it up by providing the appropriate regulations. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dennis.Hahn <@t> cookchildrens.org Tue May 6 12:18:08 2014 From: Dennis.Hahn <@t> cookchildrens.org (Dennis Hahn) Date: Tue May 6 12:18:16 2014 Subject: [Histonet] Pathologist ordering special stains/IHC's Message-ID: Currently, our histology lab is located on the opposite side of the laboratory from the pathologists. They each have an excel sheet, with scanned physician signature, on their desktop for use when they order special stains, IHC's, referral testing such as cytogenetics or EM. They fill out the case number, block, stains or testing required and print the request to the Histology printer. This form is used for cutting at the microtomes and completed and initialed by the tech completing the stains and entering the charges into MT. It is then given to the pathologist to sign as an acceptable or unacceptable stain (for our Histo QA records). Then, I review the sheet, give any feedback to the performing techs if needed, and document when the final stain was acceptable to the pathologists. The issues we are having are: 1) The pathologists may or may not print the sheet to the correct printer, since they use multiple printers throughout the day. 2) Did the pathologist actually print the order? 3) Orders may be mixed up with other items printed in Histology. 4) There is no way to track who ordered what if a sheet is missing. Did the order get put in and printed? Did it go to the wrong printer? Did histo staff misplace it? 5) There is an abundance of paper printed and shuffled back and forth daily that must be reviewed and followed up on. How are other labs accomplishing pathologists ordering these tests, tracking pending requests and QA documentation? We currently have Meditech and although we have looked extensively at it in the past, we have not found a Meditech solution for this to date. Are you using other applications such as Excel, Access, etc., or other tracking programs separately? We currently do not have any barcoding in our laboratory. Thanks for any help in advance, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 From smah2 <@t> msn.com Tue May 6 12:22:23 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Tue May 6 12:22:28 2014 Subject: [Histonet] Pathologist ordering special stains/IHC's In-Reply-To: References: Message-ID: Our pathologists dictate their requests and once transcribed, they are printed on the histology printer by transcription. This allows us to track through the LIS who transcribed and sent the request to the printer and also which printer the request was sent to. Discrepancies usually require retraining and are easily resolved. Hope this helps. Sheila Haas MicroPath Laboratories, Inc. Lakeland, FL > From: Dennis.Hahn@cookchildrens.org > To: Histonet@lists.utsouthwestern.edu > Date: Tue, 6 May 2014 17:18:08 +0000 > CC: > Subject: [Histonet] Pathologist ordering special stains/IHC's > > Currently, our histology lab is located on the opposite side of the laboratory from the pathologists. They each have an excel sheet, with scanned physician signature, on their desktop for use when they order special stains, IHC's, referral testing such as cytogenetics or EM. > They fill out the case number, block, stains or testing required and print the request to the Histology printer. This form is used for cutting at the microtomes and completed and initialed by the tech completing the stains and entering the charges into MT. It is then given to the pathologist to sign as an acceptable or unacceptable stain (for our Histo QA records). Then, I review the sheet, give any feedback to the performing techs if needed, and document when the final stain was acceptable to the pathologists. > > The issues we are having are: > > 1) The pathologists may or may not print the sheet to the correct printer, since they use multiple printers throughout the day. > > 2) Did the pathologist actually print the order? > > 3) Orders may be mixed up with other items printed in Histology. > > 4) There is no way to track who ordered what if a sheet is missing. Did the order get put in and printed? Did it go to the wrong printer? Did histo staff misplace it? > > 5) There is an abundance of paper printed and shuffled back and forth daily that must be reviewed and followed up on. > > How are other labs accomplishing pathologists ordering these tests, tracking pending requests and QA documentation? We currently have Meditech and although we have looked extensively at it in the past, we have not found a Meditech solution for this to date. Are you using other applications such as Excel, Access, etc., or other tracking programs separately? > > We currently do not have any barcoding in our laboratory. > > > Thanks for any help in advance, > Dennis > > Dennis Hahn, HT (ASCP) > Histology Lab Supervisor > Cook Children's Medical Center > 801 7th Avenue > Ft. Worth, TX 76104 > (682) 885-6133 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Tue May 6 12:25:08 2014 From: suetp918 <@t> comcast.net (Sue) Date: Tue May 6 12:25:38 2014 Subject: [Histonet] Pathologist ordering special stains/IHC's In-Reply-To: References: Message-ID: Sorry 4 ur issues we use order entry in copath which is done at the path desk the techs than call 4 a log print and work from that logs r held 4 1 month Sue TJUH Sent from my iPhone > On May 6, 2014, at 1:18 PM, Dennis Hahn wrote: > > Currently, our histology lab is located on the opposite side of the laboratory from the pathologists. They each have an excel sheet, with scanned physician signature, on their desktop for use when they order special stains, IHC's, referral testing such as cytogenetics or EM. > They fill out the case number, block, stains or testing required and print the request to the Histology printer. This form is used for cutting at the microtomes and completed and initialed by the tech completing the stains and entering the charges into MT. It is then given to the pathologist to sign as an acceptable or unacceptable stain (for our Histo QA records). Then, I review the sheet, give any feedback to the performing techs if needed, and document when the final stain was acceptable to the pathologists. > > The issues we are having are: > > 1) The pathologists may or may not print the sheet to the correct printer, since they use multiple printers throughout the day. > > 2) Did the pathologist actually print the order? > > 3) Orders may be mixed up with other items printed in Histology. > > 4) There is no way to track who ordered what if a sheet is missing. Did the order get put in and printed? Did it go to the wrong printer? Did histo staff misplace it? > > 5) There is an abundance of paper printed and shuffled back and forth daily that must be reviewed and followed up on. > > How are other labs accomplishing pathologists ordering these tests, tracking pending requests and QA documentation? We currently have Meditech and although we have looked extensively at it in the past, we have not found a Meditech solution for this to date. Are you using other applications such as Excel, Access, etc., or other tracking programs separately? > > We currently do not have any barcoding in our laboratory. > > > Thanks for any help in advance, > Dennis > > Dennis Hahn, HT (ASCP) > Histology Lab Supervisor > Cook Children's Medical Center > 801 7th Avenue > Ft. Worth, TX 76104 > (682) 885-6133 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rleyva <@t> ucsd.edu Tue May 6 13:38:22 2014 From: rleyva <@t> ucsd.edu (Ricardo Leyva) Date: Tue May 6 13:38:29 2014 Subject: [Histonet] Wrinkles on Glutaraldehyde Fixed Tissue Message-ID: Dear Histonetter, I am having an issue getting rid of wrinkles on sections from 4% glutaraldehyde fixed paraffin embedded ocular tissue. The laboratory's tissue of interest is the retina, and I use glut to avoid retinal detachment (this is mainly on tissue that won't be immunostained). I normally section at 5?m, and fix the tissue 1 or 2 days. I set the water bath at a temperature that is 5-10 C below the wax melting point. I have tried adding ethyl alcohol to the water and the wrinkles remain?or takes very long for most of them to be gone. I have tried placing the sections in a 1:15 mix of ethanol and water?and have also tried 30% ethanol in water, both at RT, before placing the sections on the water bath; when I do this, the sections separate abruptly (once in the water bath) from the slide I use for transferring the tissue, causing tissue layer separation. When I work with 10% formalin, the section wrinkles disappear nicely in the water bath that contains water and a bit ethanol. Nonetheless, when it comes to 4% glut it just does not work. Any input in troubleshooting this is greatly appreciated. Best, Ricardo Leyva (858) 822-1629 rleyva@ucsd.edu UCSD Shiley Eye Center From Michael.LaFriniere <@t> ccplab.com Wed May 7 07:19:58 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Wed May 7 07:20:13 2014 Subject: [Histonet] Slide oven temperature In-Reply-To: <8D1368E1780B9BE-3DE0-1E0A7@webmail-d225.sysops.aol.com> References: <8D1368E1780B9BE-3DE0-1E0A7@webmail-d225.sysops.aol.com> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2966D63C@AHCMSASEXCH02.my.ahc.local> 60-68 degrees C ranges are preferred, above that temperature one can begin to cause many problems with; staining, nuclear bubbling, antigen retrieval to name a few problems that have been recorded. Peggy Wink does a great 30 min CEU presentation on some of these problems through the NSH Webinar Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Monday, May 05, 2014 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide oven temperature Can someone tell me the range you set your slide drying oven at and what you base it on. thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Wed May 7 09:13:59 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed May 7 09:14:04 2014 Subject: [Histonet] RE: Wrinkles on Glutaraldehyde Fixed Tissue In-Reply-To: References: Message-ID: Why do you use glutaraldehyde for eyes? Davidson's is a much better fixative for eyes. With Davidson's you have two fast penetrating ingredients and one slow acting fixative. Glut is only a fast fixative. Besides, the best institutions who deal with eyes only use Davidson's. Just my thoughts. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ricardo Leyva Sent: Tuesday, May 06, 2014 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wrinkles on Glutaraldehyde Fixed Tissue Dear Histonetter, I am having an issue getting rid of wrinkles on sections from 4% glutaraldehyde fixed paraffin embedded ocular tissue. The laboratory's tissue of interest is the retina, and I use glut to avoid retinal detachment (this is mainly on tissue that won't be immunostained). I normally section at 5?m, and fix the tissue 1 or 2 days. I set the water bath at a temperature that is 5-10 C below the wax melting point. I have tried adding ethyl alcohol to the water and the wrinkles remain-or takes very long for most of them to be gone. I have tried placing the sections in a 1:15 mix of ethanol and water-and have also tried 30% ethanol in water, both at RT, before placing the sections on the water bath; when I do this, the sections separate abruptly (once in the water bath) from the slide I use for transferring the tissue, causing tissue layer separation. When I work with 10% formalin, the section wrinkles disappear nicely in the water bath that contains water and a bit ethanol. Nonetheless, when it comes to 4% glut it just does not work. Any input in troubleshooting this is greatly appreciated. Best, Ricardo Leyva (858) 822-1629 rleyva@ucsd.edu UCSD Shiley Eye Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From delsuec <@t> gmail.com Wed May 7 11:48:27 2014 From: delsuec <@t> gmail.com (Deloris Carter) Date: Wed May 7 11:48:30 2014 Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer Message-ID: Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center From naje1972 <@t> yahoo.com Wed May 7 11:52:41 2014 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Wed May 7 12:00:43 2014 Subject: [Histonet] (no subject) Message-ID: <1399481561.31655.YahooMailNeo@web160604.mail.bf1.yahoo.com> My name is Cynthia James; I work in a histopathology lab?in Melrose Park Illinois. We are told to change the solution(2-Methyl Butane) which?we use in the Histobath to freeze tissues for frozen sections to something less flammable. I remember a solution was?discussed that can be used instead of the?which is less flammable than the ?IsoPentane that we are currently using. Would someone contact at 708-681-3200 ext:2012 or 2036 and tell me more about this solution.I am available from 5:00 am to 1:30 pm monday-friday central time, if you know the company that is selling this solution please let me know. ? Thank you in advance Cynthia James ? P.S I can also be reached at this email address naje1972@yahoo.com or Cynthia_Haynes-James@luhs.org From liz <@t> premierlab.com Wed May 7 12:34:43 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed May 7 12:34:53 2014 Subject: [Histonet] Award Spotlight - Ann Preece Scholarship Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05A9E@SBS2K8.premierlab.local> Awards Spotlight - Ann Preece Scholarship PolySciences, Inc. sponsors this $1000 award on a reimbursement basis. Polysciences has been sponsoring this award since 2004, Ann Preece passed in 2003. She was born November 12, 1923 in Boston, Massachusetts. She worked at the pathology lab at Scripps Memorial Hospital and authored A Manual for Histological Technician which was published in three editions by Little, Brown & Co. I'm old and lucky enough to have a copy of her book in my lab and I'm sure many others out there may also have a copy. Award Criteria: This award will be given annually to a qualified applicant who has excelled in working with calcified/decalcified bone, other materials such as stents in vascular research or bioengineering development. The specimens can be human or animal in origin. This award will support educational or professional certification programs that are intended to: 1. Enhance techniques and methods presently utilized in the nominee's institution, and/or 2. Support the study of new techniques under consideration for adoption in the nominee's laboratory, and/or 3. Support continuing education or certification. The past recipients of this award are Sarah Mack, Carol Bain, Damien Laudier, Robert Skinner, Vicki Kalscheur, Linda Jenkins, Jack Radcliff, Helen Wimer, Gayle Callis and Nancy Troiano. I know anyone who reads this list will know that these individuals are recognized as the experts in the field with respects to bioengineered constructs, stents, undecalcifed and decalcified bone. If you know of anyone who excels and is passionate in this specialized area of histology then nominate them for this award. http://nsh.org/scholarships-awards/education/individual%20scholarships Happy Hump Day! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From sheryl.stephenson <@t> ag.state.nj.us Wed May 7 13:14:38 2014 From: sheryl.stephenson <@t> ag.state.nj.us (Stephenson, Sheryl) Date: Wed May 7 13:14:56 2014 Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer In-Reply-To: References: Message-ID: Lol, I had the same problem. Yes the manual does not thoroughly explain this. U have to delete the protocol from the active stains list, edit the protocol and make sure reagents are linked to the correct buckets, then add the protocol back to the active stain list. I'm not sitting in front of my stainer right now to walk you through screen by screen but that is the whole idea behind it. Delete it, edit it, then add it back. (When you delete it, it's not deleted from the stainer its only deleted from being an active stain protocol.) I hope this helps a little. If you need further help, let me know I'll go to my machine and get the step by step info for you. You can also call their customer service # and they should be able to walk u thru it. Thanks, Sheryl Stephenson Histotechnologist NJ Department of Agriculture Animal Health Diagnostic Labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Wednesday, May 07, 2014 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histogirl4 <@t> gmail.com Wed May 7 13:05:52 2014 From: histogirl4 <@t> gmail.com (histogirl4@gmail.com) Date: Wed May 7 13:18:14 2014 Subject: [Histonet] (no subject) Message-ID: <536a78e1.6572320a.47ec.799b@mx.google.com> Job Opportunity: Pathology Associates Of Greenville Texas is seeking a full-time Histotehnologist/Histotechnichian, to join out practice. We are a free standing anatomic pathology laboratory with hospital affiliations, staffed by five pathologists and two full-time Histotechnologists/Histotechnicians. Our laboratory is fully automated. We preform routine surgical pathology, few non-gyn cytology, special stains (Histochemistry) and Immunohistchemistry (IHC). Requirements for the successful applicant: Strong work ethics- Excellent Interpersonal Skills and willing to assist in Frozen sections and Maintain Policies and Procedures of The Histology Laboratory. Candidates are preferably ASCP Certified/Eligible. Interested applicants may fax their resume at 903-454-1716 E-mail address: ebrmo@yahoo.com Sent from Windows Mail From amber.mckenzie <@t> gastrodocs.net Wed May 7 13:34:01 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed May 7 13:34:05 2014 Subject: [Histonet] Slide Ovens In-Reply-To: <817715931.1523521.1398806900934.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> References: <761E2B5697F795489C8710BCC72141FF3677D39A@ex07.net.ucsf.edu> <817715931.1523521.1398806900934.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: <5A33C952BB67F4468AF1F36D739212BC011248A270@JERRY.Gia.com> Does anyone use the Thermo High-Capacity Section Dryer Item number B3120202? I've never seen it in person and I was debating on getting it vs the Cardinal one: M7289-20 SD-II-120 Slide dryer II. Any advice? Thanks! From lcolbert <@t> pathmdlabs.com Wed May 7 13:31:54 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed May 7 13:35:43 2014 Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer In-Reply-To: References: Message-ID: <12ECD7346266D74691EC2BFC75285E452F4748CA@BFL323E10.pathmdlabs.local> Call Thermo and talk to Sherry Anderson at x562. She is awesome! She just walked me through some programming issues this morning. Thermo (800) 522-7270 x562 Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Wednesday, May 07, 2014 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From James.E.Nutter <@t> questdiagnostics.com Wed May 7 16:29:11 2014 From: James.E.Nutter <@t> questdiagnostics.com (Nutter, James E) Date: Wed May 7 16:30:51 2014 Subject: [Histonet] Dako Hercept Kits Message-ID: Hello everyone, Has anyone had a problem with recent lots of Dako Hercept Kits? Thank you, James E. Nutter Jr. BS, HT & QIHC(ASCP) Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.| Chantilly Va. USA | phone 703.802.6900 x65782| fax 703.802.7191| | James.E.Nutter@QuestDiagnostics.com | www.NicholsInstitute.com ______________________________________________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From awill.imdpathlab <@t> yahoo.com Wed May 7 19:10:36 2014 From: awill.imdpathlab <@t> yahoo.com (awill.imdpathlab@yahoo.com) Date: Wed May 7 19:13:21 2014 Subject: [Histonet] Unsubscribe from emails Message-ID: <1399507836.10559.YahooMailAndroidMobile@web126103.mail.ne1.yahoo.com> Can you unsubscribe me from all the emails or tell me where to go to do so, please.? Sent from Yahoo Mail on Android From Cameron.Nowell <@t> monash.edu Wed May 7 21:49:11 2014 From: Cameron.Nowell <@t> monash.edu (Cameron Nowell) Date: Wed May 7 21:49:24 2014 Subject: [Histonet] Cryo Macrotome Message-ID: <69A36BE875B3674093D86BD7E716D57C0E4C57@PRK-MBX-04.vcp.local> Hi List, We are looking at doing whole mouse sectioning for imaging on a beta imaging system. Does anyone know of any other cryo macrotome systems other than the Leica CM3600 (http://www.leicabiosystems.com/specimen-preparation/sectioning/cryosectioning/details/product/leica-cm3600-xp/) ? It is way too large for what we need (only mice/rats) and way too expensive. Alternatively is there a standard macrotome out there for doing this sort of sectioning? What do people use for cutting full slices of human brain? Cheers Cam Cameron J. Nowell Research Facilities Manager Monash Institute of Pharmaceutical Sciences Monash University 399 Royal Parade (Mail address: 381 Royal Parade) Parkville, VIC, 3052 Australia Email: cameron.nowell@monash.edu Mobile: +61 422882700 Office: +61 9903 9587 LinkedIn: Profile Research Gate: Profile From christina.kreutzer01 <@t> gmail.com Thu May 8 04:27:32 2014 From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer) Date: Thu May 8 04:27:42 2014 Subject: [Histonet] cryoprotection and anti.freeze Message-ID: Dear Members, I was wondering what cryoprotection protocols you are using for perfusion fixed tissues (30% succrose, 1:1 Tissue Tek/PBS etc). Why are you using what you are using and did you ever have any problems. Second, I wanted your opinion concerning leaving tisse in cryoprotection solution for several months and antibody reactivity and/or if somebody ever had problems with antibody reactivity on CPS (Glycerin, Ethylene Glycol, PO4 buffer and water). Thanks in advance Christina From Lesley.Bechtold <@t> jax.org Thu May 8 07:45:51 2014 From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold) Date: Thu May 8 07:45:58 2014 Subject: [Histonet] Histology Service Supervisory Position open Message-ID: <08997064E2075247A4DD5A035DB51CDF64F89F77@jaxbhexms01.jax.org> We have an opening for a Histology Service Supervisor position. Please see the posting below. Supervisor, Histology Services The Histology Service Supervisor is responsible for day-to-day oversight of the Histology Service. This includes supervision of facility staff, work flow oversight and contributing to the completion of the overall workload in the Histology Service. This includes supervision of facility staff, providing training and guidance as needed. This position also includes carrying out routine and advanced histological procedures and contributing to completion of the overall workload in the department. Required Skills: * Excellent verbal and written communication skills. * Must be able to communicate effectively with others verbally and in writing to clearly and effectively exchange information specifically in the development of protocols and interpretation of data. * Current computer skills including use of spreadsheet, word processing, e-mail and internet tools. Must be able to utilize electronic databases and information management systems to record, monitor and analyze workflow. * Must be able to function effectively in a team environment and work with frequent changes in schedules and priorities. Required Experience: * Bachelor's degree in a biological science with HT (ASCP) or HTL (ASCP) certification and eight to ten (8-10) years of histology experience as a certified Histotechnologist. * Experience with histological techniques using animal models is desirable. * Certification in immunohistochemistry (QIHC) is required although equivalent experience (2 or more years) in immunohistochemical techniques may be considered. * Three to five (3-5) years of supervisory or lead experience. * Supervisory or lead experience in a clinical or pharmaceutical setting is desirable. Interested applicants should apply on line at www.jax.org/careers, referring to job posting #4377 with a cover letter and resume. Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From mundayscott <@t> gmavt.net Thu May 8 09:05:26 2014 From: mundayscott <@t> gmavt.net (scott munday) Date: Thu May 8 09:05:34 2014 Subject: [Histonet] Fisher Embedding Meda Message-ID: We have some extra Fisher Scientific Embedding Media for sale. It's new in the box and I have 2 cases. Each case has 4 five pound cartons in it. If anyone needs it, I have posted a link to the listing on Ebay below. http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=151297973845 -- Scott Munday Munday Scientific Instrument Service 90 Misha Lane Sanford, NC 27330 Phone: 919-775-5596 Fax: 919-776-9566 From jblaine <@t> astrixinc.com Thu May 8 10:56:32 2014 From: jblaine <@t> astrixinc.com (Jason Blaine) Date: Thu May 8 10:56:38 2014 Subject: [Histonet] Position open in Boston Suburb in Pharma/Biotech Message-ID: Hi. I am a scientific/pharma recruiter. One of my pharmaceutical/biotech customers is seeking to hire a contract IHC specialist. This is great opportunity to move out of a hospital setting or a processing lab (LabCorp, Quest, etc.) and in to a research/industry environment. Your efforts will support research and development of cancer and infectious disease drug candidates. This position is full-time, M-F, 9 to 5 (approx) and located in Waltham, MA just off of 95. If you are interested please send me a copy of your resum? (in MS Word format) to jblaine@astrixinc.com. Please also include your desired compensation. If this position is not right for you but you know of colleagues that may be more appropriate please forward them this information. Thanks! Below is a list of responsibilities: * Performs microtomy of formalin fixed paraffin embedded (FFPE) tissues. * Provides tissue processing and embedding support to all projects and programs including Discovery, Biologics, Safety Assessment, Cancer and Infectious Diseases. * Reviews and interprets Immunohistochemical and H&E stained tissue sections by light microscopy and fluorescence microscopy for Cancer and Safety Assessment projects. * Provides biomarker support by performing routine staining of tissue specimens utilizing automated and manual IHC procedures. * Implement quantitative image analysis for dual stained IHC slides. * Primary work interactions with the pathologist as well as the in vivo bioscientists * Prepares data presentations to project meetings * Work with histopathology management to lead the implementation of the quantitative analysis of image data for IHC including localization and expression patterns in rodent preclnical tissue. * Document preparation including IHC validation reports and SOPs Jason Blaine Managing Director Email: jblaine@AstrixInc.com Creating Value and Trust in the Business of Science? CONFIDENTIALITY NOTICE: The information contained in this message may be CONFIDENTIAL and is for the intended addressee only. Any unauthorized use, dissemination of the information, or copying of this message is prohibited. If you are not the intended addressee, please notify the sender immediately and delete this message. From b-frederick <@t> northwestern.edu Thu May 8 11:22:13 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu May 8 11:23:13 2014 Subject: [Histonet] CAP at NU Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0A17B4@evcspmbx2.ads.northwestern.edu> Oh yay! CAP is visiting us today. Why do they send an MT to do histo? And a snarky one at that...... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From tkngflght <@t> yahoo.com Thu May 8 12:05:57 2014 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu May 8 12:06:06 2014 Subject: [Histonet] Special stain validations Message-ID: <1399568757.8373.YahooMailNeo@web161202.mail.bf1.yahoo.com> Help! Looking for CAP regs on validating a new platform for special stains. ?All I can find is IHC.? Thank you in advance. Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From BZIMMERM <@t> gru.edu Thu May 8 12:16:49 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu May 8 12:17:00 2014 Subject: [Histonet] RE: Histonet Digest, Vol 126, Issue 9 In-Reply-To: <20140508170632.AA2A0990FDD@sms2.gru.edu> References: <20140508170632.AA2A0990FDD@sms2.gru.edu> Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF84F8FB@EX-MLB-03.ad.georgiahealth.edu> I'm a medical technologist working in anatomic pathology and I take offense to your post, Bernice. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 08, 2014 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 126, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. (no subject) (cynthia haynes) 2. Award Spotlight - Ann Preece Scholarship (Elizabeth Chlipala) 3. RE: Edit protocol for Shandon Gemini Varistainer (Stephenson, Sheryl) 4. (no subject) (histogirl4@gmail.com) 5. Slide Ovens (Amber McKenzie) 6. RE: Edit protocol for Shandon Gemini Varistainer (Laurie Colbert) 7. Dako Hercept Kits (Nutter, James E) 8. Unsubscribe from emails (awill.imdpathlab@yahoo.com) 9. Cryo Macrotome (Cameron Nowell) 10. cryoprotection and anti.freeze (Christina Kreutzer) 11. Histology Service Supervisory Position open (Lesley Bechtold) 12. Fisher Embedding Meda (scott munday) 13. Position open in Boston Suburb in Pharma/Biotech (Jason Blaine) 14. CAP at NU (Bernice Frederick) ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 May 2014 09:52:41 -0700 (PDT) From: cynthia haynes Subject: [Histonet] (no subject) To: "Histonet@lists.utsouthwestern.edu" Message-ID: <1399481561.31655.YahooMailNeo@web160604.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My name is Cynthia James; I work in a histopathology lab?in Melrose Park Illinois. We are told to change the solution(2-Methyl Butane) which?we use in the Histobath to freeze tissues for frozen sections to something less flammable. I remember a solution was?discussed that can be used instead of the?which is less flammable than the ?IsoPentane that we are currently using. Would someone contact at 708-681-3200 ext:2012 or 2036 and tell me more about this solution.I am available from 5:00 am to 1:30 pm monday-friday central time, if you know the company that is selling this solution please let me know. ? Thank you in advance Cynthia James ? P.S I can also be reached at this email address naje1972@yahoo.com or Cynthia_Haynes-James@luhs.org ------------------------------ Message: 2 Date: Wed, 7 May 2014 11:34:43 -0600 From: Elizabeth Chlipala Subject: [Histonet] Award Spotlight - Ann Preece Scholarship To: "'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05A9E@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Awards Spotlight - Ann Preece Scholarship PolySciences, Inc. sponsors this $1000 award on a reimbursement basis. Polysciences has been sponsoring this award since 2004, Ann Preece passed in 2003. She was born November 12, 1923 in Boston, Massachusetts. She worked at the pathology lab at Scripps Memorial Hospital and authored A Manual for Histological Technician which was published in three editions by Little, Brown & Co. I'm old and lucky enough to have a copy of her book in my lab and I'm sure many others out there may also have a copy. Award Criteria: This award will be given annually to a qualified applicant who has excelled in working with calcified/decalcified bone, other materials such as stents in vascular research or bioengineering development. The specimens can be human or animal in origin. This award will support educational or professional certification programs that are intended to: 1. Enhance techniques and methods presently utilized in the nominee's institution, and/or 2. Support the study of new techniques under consideration for adoption in the nominee's laboratory, and/or 3. Support continuing education or certification. The past recipients of this award are Sarah Mack, Carol Bain, Damien Laudier, Robert Skinner, Vicki Kalscheur, Linda Jenkins, Jack Radcliff, Helen Wimer, Gayle Callis and Nancy Troiano. I know anyone who reads this list will know that these individuals are recognized as the experts in the field with respects to bioengineered constructs, stents, undecalcifed and decalcified bone. If you know of anyone who excels and is passionate in this specialized area of histology then nominate them for this award. http://nsh.org/scholarships-awards/education/individual%20scholarships Happy Hump Day! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ------------------------------ Message: 3 Date: Wed, 7 May 2014 18:14:38 +0000 From: "Stephenson, Sheryl" Subject: RE: [Histonet] Edit protocol for Shandon Gemini Varistainer To: Deloris Carter , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" Lol, I had the same problem. Yes the manual does not thoroughly explain this. U have to delete the protocol from the active stains list, edit the protocol and make sure reagents are linked to the correct buckets, then add the protocol back to the active stain list. I'm not sitting in front of my stainer right now to walk you through screen by screen but that is the whole idea behind it. Delete it, edit it, then add it back. (When you delete it, it's not deleted from the stainer its only deleted from being an active stain protocol.) I hope this helps a little. If you need further help, let me know I'll go to my machine and get the step by step info for you. You can also call their customer service # and they should be able to walk u thru it. Thanks, Sheryl Stephenson Histotechnologist NJ Department of Agriculture Animal Health Diagnostic Labs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Wednesday, May 07, 2014 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 7 May 2014 18:05:52 +0000 From: Subject: [Histonet] (no subject) To: " histonet@lists.utsouthwestern.edu " Message-ID: <536a78e1.6572320a.47ec.799b@mx.google.com> Content-Type: text/plain; charset="utf-8" Job Opportunity: Pathology Associates Of Greenville Texas is seeking a full-time Histotehnologist/Histotechnichian, to join out practice. We are a free standing anatomic pathology laboratory with hospital affiliations, staffed by five pathologists and two full-time Histotechnologists/Histotechnicians. Our laboratory is fully automated. We preform routine surgical pathology, few non-gyn cytology, special stains (Histochemistry) and Immunohistchemistry (IHC). Requirements for the successful applicant: Strong work ethics- Excellent Interpersonal Skills and willing to assist in Frozen sections and Maintain Policies and Procedures of The Histology Laboratory. Candidates are preferably ASCP Certified/Eligible. Interested applicants may fax their resume at 903-454-1716 E-mail address: ebrmo@yahoo.com Sent from Windows Mail ------------------------------ Message: 5 Date: Wed, 7 May 2014 18:34:01 +0000 From: Amber McKenzie Subject: [Histonet] Slide Ovens Cc: Histonet Message-ID: <5A33C952BB67F4468AF1F36D739212BC011248A270@JERRY.Gia.com> Content-Type: text/plain; charset="utf-8" Does anyone use the Thermo High-Capacity Section Dryer Item number B3120202? I've never seen it in person and I was debating on getting it vs the Cardinal one: M7289-20 SD-II-120 Slide dryer II. Any advice? Thanks! ------------------------------ Message: 6 Date: Wed, 7 May 2014 18:31:54 +0000 From: Laurie Colbert Subject: RE: [Histonet] Edit protocol for Shandon Gemini Varistainer To: Deloris Carter , "histonet@lists.utsouthwestern.edu" Message-ID: <12ECD7346266D74691EC2BFC75285E452F4748CA@BFL323E10.pathmdlabs.local> Content-Type: text/plain; charset="utf-8" Call Thermo and talk to Sherry Anderson at x562. She is awesome! She just walked me through some programming issues this morning. Thermo (800) 522-7270 x562 Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Wednesday, May 07, 2014 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 7 May 2014 17:29:11 -0400 From: "Nutter, James E" Subject: [Histonet] Dako Hercept Kits To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello everyone, Has anyone had a problem with recent lots of Dako Hercept Kits? Thank you, James E. Nutter Jr. BS, HT & QIHC(ASCP) Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.| Chantilly Va. USA | phone 703.802.6900 x65782| fax 703.802.7191| | James.E.Nutter@QuestDiagnostics.com | www.NicholsInstitute.com ______________________________________________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ------------------------------ Message: 8 Date: Wed, 7 May 2014 17:10:36 -0700 (PDT) From: "awill.imdpathlab@yahoo.com" Subject: [Histonet] Unsubscribe from emails To: "Histonet@lists.utsouthwestern.edu" Message-ID: <1399507836.10559.YahooMailAndroidMobile@web126103.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Can you unsubscribe me from all the emails or tell me where to go to do so, please.? Sent from Yahoo Mail on Android ------------------------------ Message: 9 Date: Thu, 08 May 2014 02:49:11 +0000 From: Cameron Nowell Subject: [Histonet] Cryo Macrotome To: "histonet@lists.utsouthwestern.edu" Message-ID: <69A36BE875B3674093D86BD7E716D57C0E4C57@PRK-MBX-04.vcp.local> Content-Type: text/plain; charset="us-ascii" Hi List, We are looking at doing whole mouse sectioning for imaging on a beta imaging system. Does anyone know of any other cryo macrotome systems other than the Leica CM3600 (http://www.leicabiosystems.com/specimen-preparation/sectioning/cryosectioning/details/product/leica-cm3600-xp/) ? It is way too large for what we need (only mice/rats) and way too expensive. Alternatively is there a standard macrotome out there for doing this sort of sectioning? What do people use for cutting full slices of human brain? Cheers Cam Cameron J. Nowell Research Facilities Manager Monash Institute of Pharmaceutical Sciences Monash University 399 Royal Parade (Mail address: 381 Royal Parade) Parkville, VIC, 3052 Australia Email: cameron.nowell@monash.edu Mobile: +61 422882700 Office: +61 9903 9587 LinkedIn: Profile Research Gate: Profile ------------------------------ Message: 10 Date: Thu, 8 May 2014 11:27:32 +0200 From: Christina Kreutzer Subject: [Histonet] cryoprotection and anti.freeze To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Dear Members, I was wondering what cryoprotection protocols you are using for perfusion fixed tissues (30% succrose, 1:1 Tissue Tek/PBS etc). Why are you using what you are using and did you ever have any problems. Second, I wanted your opinion concerning leaving tisse in cryoprotection solution for several months and antibody reactivity and/or if somebody ever had problems with antibody reactivity on CPS (Glycerin, Ethylene Glycol, PO4 buffer and water). Thanks in advance Christina ------------------------------ Message: 11 Date: Thu, 8 May 2014 12:45:51 +0000 From: Lesley Bechtold Subject: [Histonet] Histology Service Supervisory Position open To: "histonet@lists.utsouthwestern.edu" Message-ID: <08997064E2075247A4DD5A035DB51CDF64F89F77@jaxbhexms01.jax.org> Content-Type: text/plain; charset="us-ascii" We have an opening for a Histology Service Supervisor position. Please see the posting below. Supervisor, Histology Services The Histology Service Supervisor is responsible for day-to-day oversight of the Histology Service. This includes supervision of facility staff, work flow oversight and contributing to the completion of the overall workload in the Histology Service. This includes supervision of facility staff, providing training and guidance as needed. This position also includes carrying out routine and advanced histological procedures and contributing to completion of the overall workload in the department. Required Skills: * Excellent verbal and written communication skills. * Must be able to communicate effectively with others verbally and in writing to clearly and effectively exchange information specifically in the development of protocols and interpretation of data. * Current computer skills including use of spreadsheet, word processing, e-mail and internet tools. Must be able to utilize electronic databases and information management systems to record, monitor and analyze workflow. * Must be able to function effectively in a team environment and work with frequent changes in schedules and priorities. Required Experience: * Bachelor's degree in a biological science with HT (ASCP) or HTL (ASCP) certification and eight to ten (8-10) years of histology experience as a certified Histotechnologist. * Experience with histological techniques using animal models is desirable. * Certification in immunohistochemistry (QIHC) is required although equivalent experience (2 or more years) in immunohistochemical techniques may be considered. * Three to five (3-5) years of supervisory or lead experience. * Supervisory or lead experience in a clinical or pharmaceutical setting is desirable. Interested applicants should apply on line at www.jax.org/careers, referring to job posting #4377 with a cover letter and resume. Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ------------------------------ Message: 12 Date: Thu, 8 May 2014 10:05:26 -0400 From: scott munday Subject: [Histonet] Fisher Embedding Meda To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 We have some extra Fisher Scientific Embedding Media for sale. It's new in the box and I have 2 cases. Each case has 4 five pound cartons in it. If anyone needs it, I have posted a link to the listing on Ebay below. http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=151297973845 -- Scott Munday Munday Scientific Instrument Service 90 Misha Lane Sanford, NC 27330 Phone: 919-775-5596 Fax: 919-776-9566 ------------------------------ Message: 13 Date: Thu, 8 May 2014 15:56:32 +0000 From: Jason Blaine Subject: [Histonet] Position open in Boston Suburb in Pharma/Biotech To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi. I am a scientific/pharma recruiter. One of my pharmaceutical/biotech customers is seeking to hire a contract IHC specialist. This is great opportunity to move out of a hospital setting or a processing lab (LabCorp, Quest, etc.) and in to a research/industry environment. Your efforts will support research and development of cancer and infectious disease drug candidates. This position is full-time, M-F, 9 to 5 (approx) and located in Waltham, MA just off of 95. If you are interested please send me a copy of your resum? (in MS Word format) to jblaine@astrixinc.com. Please also include your desired compensation. If this position is not right for you but you know of colleagues that may be more appropriate please forward them this information. Thanks! Below is a list of responsibilities: * Performs microtomy of formalin fixed paraffin embedded (FFPE) tissues. * Provides tissue processing and embedding support to all projects and programs including Discovery, Biologics, Safety Assessment, Cancer and Infectious Diseases. * Reviews and interprets Immunohistochemical and H&E stained tissue sections by light microscopy and fluorescence microscopy for Cancer and Safety Assessment projects. * Provides biomarker support by performing routine staining of tissue specimens utilizing automated and manual IHC procedures. * Implement quantitative image analysis for dual stained IHC slides. * Primary work interactions with the pathologist as well as the in vivo bioscientists * Prepares data presentations to project meetings * Work with histopathology management to lead the implementation of the quantitative analysis of image data for IHC including localization and expression patterns in rodent preclnical tissue. * Document preparation including IHC validation reports and SOPs Jason Blaine Managing Director Email: jblaine@AstrixInc.com Creating Value and Trust in the Business of Science? CONFIDENTIALITY NOTICE: The information contained in this message may be CONFIDENTIAL and is for the intended addressee only. Any unauthorized use, dissemination of the information, or copying of this message is prohibited. If you are not the intended addressee, please notify the sender immediately and delete this message. ------------------------------ Message: 14 Date: Thu, 8 May 2014 16:22:13 +0000 From: Bernice Frederick Subject: [Histonet] CAP at NU To: Histonet Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0A17B4@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Oh yay! CAP is visiting us today. Why do they send an MT to do histo? And a snarky one at that...... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 126, Issue 9 **************************************** From cjbulmer <@t> sbcglobal.net Thu May 8 12:46:08 2014 From: cjbulmer <@t> sbcglobal.net (Cindy Bulmer) Date: Thu May 8 12:46:13 2014 Subject: [Histonet] Dako Hercept Kits In-Reply-To: References: Message-ID: <1399571168.68948.YahooMailNeo@web180901.mail.ne1.yahoo.com> I'm using Dako Hercept Kit with lot number 20003874 and exp.date of 9-2014 and it is fine. Our previous kit was lot number 20003728, exp. date 8-2014 and it was fine. Hope this helps everyone. Cindy ? Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX On Wednesday, May 7, 2014 4:33 PM, "Nutter, James E" wrote: Hello everyone, Has anyone had a problem with recent lots of Dako Hercept Kits?? Thank you, James E. Nutter Jr. BS, HT & QIHC(ASCP) Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.| Chantilly Va. USA | phone 703.802.6900 x65782| fax 703.802.7191| | James.E.Nutter@QuestDiagnostics.com ? | www.NicholsInstitute.com ______________________________________________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu May 8 12:58:45 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu May 8 12:58:52 2014 Subject: [Histonet] Ann Preece Award Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05AC1@SBS2K8.premierlab.local> Hello Histonetters If you are keeping up with my Award Spotlight posts you'll know that the last award I focused on was the Ann Preece Scholarship. I just got off the phone with Ken Urban and we had a great chat, apparently prior to Polysciences sponsoring this award through the NSH, Ann Preece herself used to sponsor this award. Ken remembers receiving this award in the mid to late 1960's. He submitted a picture of a Movats Pentachrome performed on an undecalcifed bone sample. The award at that time consisted of a certificate, a copy of Ann's book; A Manual for Histologic Technicians and a $100.00. I just searched and what do you know -- this book is still available on line, just check out this link - http://www.amazon.com/Manual-Histologic-Technicians-Ann-Preece/dp/0316717657 that's just amazing. Well the reason for this post is that I wanted to update everyone with what I found out and that I would like to get some more info on this award just to fill in the blanks, so if there is anyone out there that can remember anyone else who received this award, or the dates it was given, etc. it would be great if you could e-mail me. Any information or stories about Ann would be appreciated. It's nice to know where these awards came from. Thanks and have a great day. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From rleyva <@t> ucsd.edu Thu May 8 13:16:00 2014 From: rleyva <@t> ucsd.edu (Ricardo Leyva) Date: Thu May 8 13:16:07 2014 Subject: [Histonet] Re: Wrinkles on Glutaraldehyde Fixed Tissue In-Reply-To: Message-ID: Hi Bea, Thank you for your response. The reason to 4% Glut use is just a PI request (really a matter of preference), and it is only on tissue that will not be immunostained. The ocular tissue I work with is rabbit. One thing I noticed is that the tissue that is fixed for 24h takes longer to become wrinkle-free, whereas the 48h (or more) fixed tissue seems to spread out in the waterbath with better consistency. I am aware of the use of Davidson's?in fact you are the one who so patiently gave me recommendations on it, and for that I am grateful. Nonetheless, something I'd encounter with Davidson's each time is that tissue would shrink a bit too much after processing (all of my reagents?EtOH, Xylenes, and Wax are set for about 1h20min each); I tried decreasing the processing time but the issue remains. Perhaps the processing time needs to be increased on my end to get better results. Ricardo On 5/7/14 7:13 AM, "Bea DeBrosse-Serra" wrote: >Why do you use glutaraldehyde for eyes? Davidson's is a much better >fixative for eyes. With Davidson's you have two fast penetrating >ingredients and one slow acting fixative. Glut is only a fast fixative. >Besides, the best institutions who deal with eyes only use Davidson's. >Just my thoughts. > >Bea > >Beatrice DeBrosse-Serra HT(ASCP)QIHC >Isis Pharmaceuticals >Antisense Drug Discovery >2855 Gazelle Ct. >Carlsbad, CA 92010 >760-603-2371 > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ricardo >Leyva >Sent: Tuesday, May 06, 2014 11:38 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Wrinkles on Glutaraldehyde Fixed Tissue > >Dear Histonetter, > >I am having an issue getting rid of wrinkles on sections from 4% >glutaraldehyde fixed paraffin embedded ocular tissue. The laboratory's >tissue of interest is the retina, and I use glut to avoid retinal >detachment (this is mainly on tissue that won't be immunostained). I >normally section at 5?m, and fix the tissue 1 or 2 days. I set the water >bath at a temperature that is 5-10 C below the wax melting point. I have >tried adding ethyl alcohol to the water and the wrinkles remain-or takes >very long for most of them to be gone. I have tried placing the sections >in a 1:15 mix of ethanol and water-and have also tried 30% ethanol in >water, both at RT, before placing the sections on the water bath; when I >do this, the sections separate abruptly (once in the water bath) from the >slide I use for transferring the tissue, causing tissue layer separation. >When I work with 10% formalin, the section wrinkles disappear nicely in >the water bath that contains water and a bit ethanol. Nonetheless, when >it comes to 4% glut it just does not work. > >Any input in troubleshooting this is greatly appreciated. > >Best, > >Ricardo Leyva >(858) 822-1629 >rleyva@ucsd.edu >UCSD Shiley Eye Center > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.auge <@t> gmail.com Thu May 8 13:18:15 2014 From: tony.auge <@t> gmail.com (Tony Auge) Date: Thu May 8 13:18:19 2014 Subject: [Histonet] Employee Evaluation Message-ID: Hello Histonet, I am a relatively new supervisor and have been tasked with conducting my first employee evaluation. Does anyone have any documentation or checklists they would like to share with me to help me get started please? Thanks, Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com From AHutton <@t> dh.org Thu May 8 13:20:33 2014 From: AHutton <@t> dh.org (Hutton, Allison) Date: Thu May 8 13:20:38 2014 Subject: [Histonet] ihc billing Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A02D360@DH-MAIL01.dhorg.org> Would anybody be able to shed some light on the 2014 updated IHC billing. I understand the 88342 and 88343 codes but am looking for some clarification on the G0461 and G0462 codes. Do these pertain to multiple antibodies on one slide as the 88343 does or is the G0461 for the 1st antibody and G0462 for all remaining antibodies performed for one specimen site? Thank you in advance Allison From Joyce.Weems <@t> emoryhealthcare.org Thu May 8 13:25:57 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu May 8 13:26:09 2014 Subject: [Histonet] RE: ihc billing In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64A02D360@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64A02D360@DH-MAIL01.dhorg.org> Message-ID: The G codes are for Medicare/Medicaid. G0461 - the first and G0462 - each additional per SPECIMEN. 88342 is per BLOCK. And you understand 88343... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Thursday, May 08, 2014 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ihc billing Would anybody be able to shed some light on the 2014 updated IHC billing. I understand the 88342 and 88343 codes but am looking for some clarification on the G0461 and G0462 codes. Do these pertain to multiple antibodies on one slide as the 88343 does or is the G0461 for the 1st antibody and G0462 for all remaining antibodies performed for one specimen site? Thank you in advance Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From abright <@t> brightinstruments.com Thu May 8 14:32:40 2014 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu May 8 15:44:04 2014 Subject: [Histonet] Cryo Macrotome In-Reply-To: <69A36BE875B3674093D86BD7E716D57C0E4C57@PRK-MBX-04.vcp.local> References: <69A36BE875B3674093D86BD7E716D57C0E4C57@PRK-MBX-04.vcp.local> Message-ID: <669C08EE-6B55-4663-99F0-F017F5B9F592@brightinstruments.com> Dear Cam, I can help you on this as we have supplied the Bright 5040 OTF/AS, fitted with large section conversion kit & Tungsten Carbide tipped knife for this type of application. It has also been used for undecalcified adult Rat head. We also manufacture the Bright 8250 cryostat for larger sections in whole human brain and full size rats. See our website www.brightinstruments.com Regards.... Alan Bright Sent from my iPhone > On 8 May 2014, at 03:52, "Cameron Nowell" wrote: > > Hi List, > > We are looking at doing whole mouse sectioning for imaging on a beta imaging system. Does anyone know of any other cryo macrotome systems other than the Leica CM3600 (http://www.leicabiosystems.com/specimen-preparation/sectioning/cryosectioning/details/product/leica-cm3600-xp/) ? > > It is way too large for what we need (only mice/rats) and way too expensive. > > Alternatively is there a standard macrotome out there for doing this sort of sectioning? What do people use for cutting full slices of human brain? > > Cheers > > Cam > > > Cameron J. Nowell > Research Facilities Manager > > Monash Institute of Pharmaceutical Sciences > Monash University > 399 Royal Parade > (Mail address: 381 Royal Parade) > Parkville, VIC, 3052 > Australia > > Email: cameron.nowell@monash.edu > Mobile: +61 422882700 > Office: +61 9903 9587 > > LinkedIn: Profile > Research Gate: Profile > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > From jclark <@t> pcnm.com Thu May 8 18:40:27 2014 From: jclark <@t> pcnm.com (Joanne Clark) Date: Thu May 8 18:40:39 2014 Subject: [Histonet] Senior Histotech Position Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4B5C32A@S10MAILD001N3.SH10.lan> We have a position open for a Senior HT/HTL, preferably with grossing experience for our Las Cruces lab. Interested parties can contact me directly for details. Joanne Clark, BAAS, HT(ASCP) Director of Histology Pathology Consultants of New Mexico 575-317-6403 jclark@pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Tony.Reilly <@t> health.qld.gov.au Thu May 8 20:17:38 2014 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Thu May 8 20:17:54 2014 Subject: [Histonet] RE: Wrinkles on Glutaraldehyde Fixed Tissue In-Reply-To: References: Message-ID: Hi Bea The problem with Glutaraldehyde is not its fixation but it's penetration rate. It is very slow to penetrate which is why EM tissue specimens must be diced to such small pieces. If you want to use Glut you will need to cut the tissue very thin and use the extended fixation time. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _________________________________________________ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ricardo Leyva Sent: Friday, 9 May 2014 4:16 AM To: Tony Reilly; Bea DeBrosse-Serra; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Wrinkles on Glutaraldehyde Fixed Tissue Hi Bea, Thank you for your response. The reason to 4% Glut use is just a PI request (really a matter of preference), and it is only on tissue that will not be immunostained. The ocular tissue I work with is rabbit. One thing I noticed is that the tissue that is fixed for 24h takes longer to become wrinkle-free, whereas the 48h (or more) fixed tissue seems to spread out in the waterbath with better consistency. I am aware of the use of Davidson's wrote: >Why do you use glutaraldehyde for eyes? Davidson's is a much better >fixative for eyes. With Davidson's you have two fast penetrating >ingredients and one slow acting fixative. Glut is only a fast fixative. >Besides, the best institutions who deal with eyes only use Davidson's. >Just my thoughts. > >Bea > >Beatrice DeBrosse-Serra HT(ASCP)QIHC >Isis Pharmaceuticals >Antisense Drug Discovery >2855 Gazelle Ct. >Carlsbad, CA 92010 >760-603-2371 > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ricardo >Leyva >Sent: Tuesday, May 06, 2014 11:38 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Wrinkles on Glutaraldehyde Fixed Tissue > >Dear Histonetter, > >I am having an issue getting rid of wrinkles on sections from 4% >glutaraldehyde fixed paraffin embedded ocular tissue. The laboratory's >tissue of interest is the retina, and I use glut to avoid retinal >detachment (this is mainly on tissue that won't be immunostained). I >normally section at 5?m, and fix the tissue 1 or 2 days. I set the >water bath at a temperature that is 5-10 C below the wax melting point. >I have tried adding ethyl alcohol to the water and the wrinkles >remain-or takes very long for most of them to be gone. I have tried >placing the sections in a 1:15 mix of ethanol and water-and have also >tried 30% ethanol in water, both at RT, before placing the sections on >the water bath; when I do this, the sections separate abruptly (once in >the water bath) from the slide I use for transferring the tissue, causing tissue layer separation. >When I work with 10% formalin, the section wrinkles disappear nicely in >the water bath that contains water and a bit ethanol. Nonetheless, when >it comes to 4% glut it just does not work. > >Any input in troubleshooting this is greatly appreciated. > >Best, > >Ricardo Leyva >(858) 822-1629 >rleyva@ucsd.edu >UCSD Shiley Eye Center > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From Michael.LaFriniere <@t> ccplab.com Fri May 9 10:11:43 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Fri May 9 10:11:55 2014 Subject: [Histonet] Special stain validations In-Reply-To: <1399568757.8373.YahooMailNeo@web161202.mail.bf1.yahoo.com> References: <1399568757.8373.YahooMailNeo@web161202.mail.bf1.yahoo.com> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2966F09E@AHCMSASEXCH02.my.ahc.local> Hi Cheryl, I would suggest validation of any testing method in histology equivalent to the IHC requirement Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A . Silver Spring, MD 20904 P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Thursday, May 08, 2014 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special stain validations Help! Looking for CAP regs on validating a new platform for special stains. ?All I can find is IHC.? Thank you in advance. Cheryl ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.LaFriniere <@t> ccplab.com Fri May 9 10:40:01 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Fri May 9 10:40:14 2014 Subject: [Histonet] RE: ihc billing In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64A02D360@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64A02D360@DH-MAIL01.dhorg.org> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2966F0D3@AHCMSASEXCH02.my.ahc.local> Allsion It is my understanding, and I have gone over this with many billing experts as well and performed endless research on. For medicare appropriate billing with multiplex anitbodies, use the GO461 for first multiplex antibody then GO462 for each "separately identified" antibody that the pathologist specifically diagnostically mentions in the report. An example; if you perform a PIN4 antibody that consist of 3 distinct antibodies the code GO461 for the first antibody mentioned; example(racemace) then GO462 for each additional separately identified antibody (examples would be the 2 cytokeratins in many cocktails). GO462 is also used for each additional antibody used on each SPECIMEN; example a tissue that was submitted a specific specimen, example "bx of left arm" Pathologist orders pan-cytoketatin, s100, ki67, for medicare you would bill GO461 for the first antibody then GO462 x 2 for the (2) additional antibodies, again the pathologist must mention each result on the pathology report. Hope this helps Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Thursday, May 08, 2014 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ihc billing Would anybody be able to shed some light on the 2014 updated IHC billing. I understand the 88342 and 88343 codes but am looking for some clarification on the G0461 and G0462 codes. Do these pertain to multiple antibodies on one slide as the 88343 does or is the G0461 for the 1st antibody and G0462 for all remaining antibodies performed for one specimen site? Thank you in advance Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.LaFriniere <@t> ccplab.com Fri May 9 10:50:40 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Fri May 9 10:50:51 2014 Subject: [Histonet] GO461 GO462 In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D2966F0D3@AHCMSASEXCH02.my.ahc.local> References: <4ED8C96A8F20FC4F883A92E2A0A0D64A02D360@DH-MAIL01.dhorg.org> <4A2A16B9707CE04E9CB6C82DC18C1D2966F0D3@AHCMSASEXCH02.my.ahc.local> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2966F115@AHCMSASEXCH02.my.ahc.local> Sorry forgot to add a specific billing issue with medicare GO461 GO462 codes If you performed (5) cytokeratins on a (1) large sentinel lymph node that was grossed in five separate blocks, under medicare you can only bill GO461 x 1 NOT x 5 like you can do for non medicare cases! Michael -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael LaFriniere Sent: Friday, May 09, 2014 11:40 AM To: Hutton, Allison; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: ihc billing Allsion It is my understanding, and I have gone over this with many billing experts as well and performed endless research on. For medicare appropriate billing with multiplex anitbodies, use the GO461 for first multiplex antibody then GO462 for each "separately identified" antibody that the pathologist specifically diagnostically mentions in the report. An example; if you perform a PIN4 antibody that consist of 3 distinct antibodies the code GO461 for the first antibody mentioned; example(racemace) then GO462 for each additional separately identified antibody (examples would be the 2 cytokeratins in many cocktails). GO462 is also used for each additional antibody used on each SPECIMEN; example a tissue that was submitted a specific specimen, example "bx of left arm" Pathologist orders pan-cytoketatin, s100, ki67, for medicare you would bill GO461 for the first antibody then GO462 x 2 for the (2) additional antibodies, again the pathologist must mention each result on the pathology report. Hope this helps Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Thursday, May 08, 2014 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ihc billing Would anybody be able to shed some light on the 2014 updated IHC billing. I understand the 88342 and 88343 codes but am looking for some clarification on the G0461 and G0462 codes. Do these pertain to multiple antibodies on one slide as the 88343 does or is the G0461 for the 1st antibody and G0462 for all remaining antibodies performed for one specimen site? Thank you in advance Allison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcman2010 <@t> hotmail.com Fri May 9 16:49:07 2014 From: ihcman2010 <@t> hotmail.com (Glen) Date: Fri May 9 16:49:20 2014 Subject: [Histonet] How do you do Histonet? Message-ID: http://familywalk.nl/wp-includes/behind.php?frqxesfvr1961bmxeew Fri, 9 May 2014 23:49:07 ####################### ihcman2010@hotmail.com So, this polyamory stuff means all the horny geek chicks at CMU get to fuck all the guys they want, and the guys have to put up with their girlfriend sleeping with other men, right? I mean, I don't see too many guys at CMU with more than one girlfriend... -- unknown CMU Escort van passenger From rleyva <@t> ucsd.edu Sat May 10 01:26:50 2014 From: rleyva <@t> ucsd.edu (Ricardo Leyva) Date: Sat May 10 01:26:56 2014 Subject: [Histonet] Re: Wrinkles on Glutaraldehyde Fixed Tissue In-Reply-To: Message-ID: Hi Tony, Your statement makes sense?glutaraldehyde is quick to fix but slow to penetrate, and I realized that when comparing the tissue fixation time frames I used (24h and 48h or more). The tissue that was fixed for 24h is very hard to spread in the water bath; on the other hand, that which was in the fixative 48h or longer spread out nicely. Hence, the 24h tissue may have gotten underfixed, making it difficult to spread and become wrinkle-free. Ricardo On 5/8/14 6:17 PM, "Tony Reilly" wrote: >Hi Bea > >The problem with Glutaraldehyde is not its fixation but it's penetration >rate. It is very slow to penetrate which is why EM tissue specimens must >be diced to such small pieces. If you want to use Glut you will need to >cut the tissue very thin and use the extended fixation time. > >Regards >Tony > > >Tony Reilly B.App.Sc, M.Sc >Chief Scientist >Anatomical Pathology >Pathology Queensland PAH >_________________________________________________ >Health Services Support Agency| Department of Health > >Building 15, Level 1, >199 Ipswich Road >WOOLLOONGABBA Queensland 4102 >Ph: 07 3176 2412 >Mob: 0402139411 >Fax: 07 3176 2930 >Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au > > > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ricardo >Leyva >Sent: Friday, 9 May 2014 4:16 AM >To: Tony Reilly; Bea DeBrosse-Serra; histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Wrinkles on Glutaraldehyde Fixed Tissue > >Hi Bea, > >Thank you for your response. The reason to 4% Glut use is just a PI >request (really a matter of preference), and it is only on tissue that >will not be immunostained. The ocular tissue I work with is rabbit. One >thing I noticed is that the tissue that is fixed for 24h takes longer to >become wrinkle-free, whereas the 48h (or more) fixed tissue seems to >spread out in the waterbath with better consistency. > >I am aware of the use of Davidson'spatiently gave me recommendations on it, and for that I am grateful. >Nonetheless, something I'd encounter with Davidson's each time is that >tissue would shrink a bit too much after processing (all of my >reagentsdecreasing the processing time but the issue remains. Perhaps the >processing time needs to be increased on my end to get better results. > > >Ricardo > > >On 5/7/14 7:13 AM, "Bea DeBrosse-Serra" >wrote: > >>Why do you use glutaraldehyde for eyes? Davidson's is a much better >>fixative for eyes. With Davidson's you have two fast penetrating >>ingredients and one slow acting fixative. Glut is only a fast fixative. >>Besides, the best institutions who deal with eyes only use Davidson's. >>Just my thoughts. >> >>Bea >> >>Beatrice DeBrosse-Serra HT(ASCP)QIHC >>Isis Pharmaceuticals >>Antisense Drug Discovery >>2855 Gazelle Ct. >>Carlsbad, CA 92010 >>760-603-2371 >> >> >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ricardo >>Leyva >>Sent: Tuesday, May 06, 2014 11:38 AM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] Wrinkles on Glutaraldehyde Fixed Tissue >> >>Dear Histonetter, >> >>I am having an issue getting rid of wrinkles on sections from 4% >>glutaraldehyde fixed paraffin embedded ocular tissue. The laboratory's >>tissue of interest is the retina, and I use glut to avoid retinal >>detachment (this is mainly on tissue that won't be immunostained). I >>normally section at 5?m, and fix the tissue 1 or 2 days. I set the >>water bath at a temperature that is 5-10 C below the wax melting point. >>I have tried adding ethyl alcohol to the water and the wrinkles >>remain-or takes very long for most of them to be gone. I have tried >>placing the sections in a 1:15 mix of ethanol and water-and have also >>tried 30% ethanol in water, both at RT, before placing the sections on >>the water bath; when I do this, the sections separate abruptly (once in >>the water bath) from the slide I use for transferring the tissue, >>causing tissue layer separation. >>When I work with 10% formalin, the section wrinkles disappear nicely in >>the water bath that contains water and a bit ethanol. Nonetheless, when >>it comes to 4% glut it just does not work. >> >>Any input in troubleshooting this is greatly appreciated. >> >>Best, >> >>Ricardo Leyva >>(858) 822-1629 >>rleyva@ucsd.edu >>UCSD Shiley Eye Center >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >************************************************************************** >****** >This email, including any attachments sent with it, is confidential and >for the sole use of the intended recipient(s). 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You >should also delete this email, and any copies, from your computer system >network and destroy any hard copies produced. >If not an intended recipient of this email, you must not copy, distribute >or take any action(s) that relies on it; any form of disclosure, >modification, distribution and/or publication of this email is also >prohibited. >Although Queensland Health takes all reasonable steps to ensure this >email does not contain malicious software, Queensland Health does not >accept responsibility for the consequences if any person's computer >inadvertently suffers any disruption to services, loss of information, >harm or is infected with a virus, other malicious computer programme or >code that may occur as a consequence of receiving this email. >Unless stated otherwise, this email represents only the views of the >sender and not the views of the Queensland Government. >************************************************************************** >******** > From JMyers1 <@t> aol.com Mon May 12 09:55:52 2014 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Mon May 12 09:55:56 2014 Subject: [Histonet] Quality Control Manager position at Biocare Medical Message-ID: <43983.2cd69ece.40a23af7@aol.com> Greetings Fellow Histonetters -- I have been asked to 'post' the following job opportunity on my employer's behalf: Biocare Medical, a leading manufacturer of IHC/ISH reagents and instruments, is seeking to fill the position of Immunohistochemistry Quality Control Manager within its Concord, California facility. The ideal candidate is currently based on the West Coast and possesses managerial and quality control/assurance experience within the immunohistochemistry laboratory. If you are interested in this opportunity, please submit your resume and cover letter to: _dtacha@biocare.net_ (mailto:dtacha@biocare.net) (Please do not respond to Joe Myers) From christina.wolfe <@t> bms.com Mon May 12 15:16:17 2014 From: christina.wolfe <@t> bms.com (Wolfe, Christina) Date: Mon May 12 15:16:24 2014 Subject: [Histonet] RE: anyone out there doing a DMAB-nitrite stain for tryptophan? Message-ID: As a follow up to my original questions - I now understand the ceolloidinise is the procedure using celloidin. I am still having trouble finding a vendor for celloidin. Can I substitute LVN in place of the celloidin? Or maybe there is another method to use in this step. Any feedback will be greatly appreciated! Kristie Christina Wolfe, BS, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 From: Wolfe, Christina Sent: Monday, May 12, 2014 2:25 PM To: 'histonet@lists.utsouthwestern.edu' Subject: anyone out there doing a DMAB-nitrite stain for tryptophan? Hi, We are wanting to do the DMAB-nitrite method for tryptophan (Bancroft is the reference). I have some questions about the method. If anyone has experience, please contact me. Below are my questions: 1. Take sections to alcohol. (What percentage? Not sure if this matters). 2. Celloidinise in 0.5 per cent celloidin. (Where can I find celloidinise - is this still sold commercially?) 3. In step 6 of the procedure there is an acid alcohol rinse (anyone know what the acid alcohol concentration should be?) Thanks in advance if you can help! Kristie Christina Wolfe, BS, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From RHEYNA <@t> lumc.edu Mon May 12 15:16:17 2014 From: RHEYNA <@t> lumc.edu (ROGER HEYNA) Date: Mon May 12 15:16:28 2014 Subject: [Histonet] PMS2 on Leica Bond III Message-ID: Hi Everyone, I'm curious about different PMS2 antibodies and staining protocols for the Leica Bond III Autostainer. I'd appreciate any feedback from individuals performing this stain on this platform, including antibody clones and staining protocols. Thanks in advance! Roger From patrick.lewis <@t> seattlechildrens.org Mon May 12 16:11:53 2014 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Mon May 12 16:12:14 2014 Subject: [Histonet] OCT Issues Message-ID: <3903BE18914F4440834F0E620415FFCA38291BDF@PPWEXD01a.childrens.sea.kids> Hi everyone. With Snap frozen tissue in OCT. If I cut sections and fix them in cold acetone for 10 minutes, (Is 2 minutes vs 10 minutes going to cause any issues?) Is it better to dry them and then put them in DPBS or just put them straight into DPBS? If I changed the fixative to 10% NBF would that be better for the tissues than Cold Acetone? I am still having problems with my Frozen sections looking digested, and I am not really sure what is causing it. Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jahern <@t> edward.org Mon May 12 17:07:24 2014 From: jahern <@t> edward.org (Ahern, Joan) Date: Mon May 12 17:07:32 2014 Subject: [Histonet] part time position in Naperville 5-10 am Message-ID: <0C1C12F52E8DFD47A890BB5ADC4991069976F64D@MXMB03.edward.org> Hi All, We have a part time histotech position at Edward Hospital in Naperville. Please contact me with questions or go to the website: Edward.org. Thanks! Joan Ahern Anatomic Pathology Supervisor 630.527.5676 Edward Hospital and Health Services 801 Washington St Naperville IL 60563 From yuchiendiana <@t> yahoo.com Mon May 12 17:37:17 2014 From: yuchiendiana <@t> yahoo.com (Yuchien) Date: Mon May 12 17:37:19 2014 Subject: [Histonet] Ventana Renaissance tissue processor manual Message-ID: We would like to set up our used Ventana Renaissance tissue processor. However, the setup and operation manual were missing. If anyone has a copy of this manual, please let us know. Much appreciated! Regards, Yu-Chien Chou, Ph.D. Ensigna Biosystems, Inc. Sent from my iPad From smcbride <@t> andrew.cmu.edu Tue May 13 10:50:22 2014 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Tue May 13 10:50:21 2014 Subject: [Histonet] change of email address Message-ID: <5BA685A5-1B17-4047-9FBC-DF8A41A6C3B5@andrew.cmu.edu> Hi Linda, I'm changing jobs, so I need to change my histonet email address. The new email address is: seanmcbride007@me.com. Thanks for all of your help, Sean McBride From klaus.dern44 <@t> gmail.com Tue May 13 11:11:38 2014 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Tue May 13 11:11:42 2014 Subject: [Histonet] THICK AND THIN SECTIONS? Message-ID: If you are using one of the following Microtomes and the advance mechanism is worn out ( too much play ). REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA / JUNG 2035 LEICA - CM-1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome, because these Models are no longer supported by the Manufacturer (no parts availability). Rather than replacing these excellent instruments, I have a permanent solution to this problem for a fraction of the cost of purchasing a new Microtome. For Information and References contact: Klaus Dern Phone: 706 635-8840 Fax: 706 635-3074 E-MAIL klaus.dern44@gmail.com From gayle.callis <@t> bresnan.net Tue May 13 11:40:18 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue May 13 11:40:30 2014 Subject: [Histonet] Re: OCT embedding problems Message-ID: <000001cf6eca$086ca220$1945e660$@bresnan.net> You Wrote: Hi everyone. With Snap frozen tissue in OCT. If I cut sections and fix them in cold acetone for 10 minutes, (Is 2 minutes vs 10 minutes going to cause any issues?) Is it better to dry them and then put them in DPBS or just put them straight into DPBS? If I changed the fixative to 10% NBF would that be better for the tissues than Cold Acetone? I am still having problems with my Frozen sections looking digested, and I am not really sure what is causing it. Patrick. **************************************************************************** ************************************************ You never said how you store your frozen section immediately after sectioning and before acetone fixation? You should NOT store frozen sections in a cryostat, a thaw/refreeze issue. Also, when you take the FS out of the cryostat, water condensation forms, and this alone can damage the morphology along with antigens. It is better to section and dry at RT. Seattle can have a very humid air, and that means more water condensation formation if you store your sections in the cryostat before acetone fixation. Drying in front of a fan is ideal. We had much better success with air drying frozen sections at RT BEFORE a minimum of 30 minutes going into 4C acetone for 10 minutes. Fixation times and temperature of acetone varies from lab to lab. Fixation time in cold acetone also depends on the antigen, some antigens do better with longer fixation at 10 min and/or longer drying at RT. We fixed human renal biopsy sections for 10 dips (approximately 10 to 15 seconds)in 4C acetone when doing immunuglobulin immunofluorescence staining. The sections were always air dried before fixation a minimum of 30 minutes or longer. After acetone fixation, the sections sit for 15 or so to allow acetone to evaporate before staining or storage at -80C. We found water was the enemy with frozen sections destined for solvent fixation. However, if you are going to stain with an H&E or for antigens that withstand NBF fixation, then you can put the section immediately into RT NBF for an hour to overnight, or some set optimized time. Remember NBF needs time to the crosslinking of proteins, and 1 minute may not be ideal . Since it takes a bit of time to do the actual sectioning, by the time you finish with the last section, the first one immersed in NBF may be adequately fixed but keep track of approximate time. It is a good idea to optimize NBF fixation time. With longer NBF fixation and doing an H&E stain, the longer it fixed the better the section looked, plus was stable on the slide (no lift off). Overnight NBF fixation was popular for H&E if it wasn't for rapid diagnosis. Our frozens looked very much like FFPE unless there was incorrect, slow snap freeze causing freezing artifact. Gayle Callis HT/HTL/MT(ASCP) From Dennis.Hahn <@t> cookchildrens.org Tue May 13 13:00:59 2014 From: Dennis.Hahn <@t> cookchildrens.org (Dennis Hahn) Date: Tue May 13 13:01:12 2014 Subject: [Histonet] Staining for Acinar vs Islet Cells in FS Message-ID: Does anyone have a procedure they would be willing to share for a rapid special stain to distinguish acinar from islet cells in frozen sections? Thanks, Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 From Leah.Nichols <@t> cls.ab.ca Tue May 13 15:56:29 2014 From: Leah.Nichols <@t> cls.ab.ca (Leah Nichols) Date: Tue May 13 15:58:12 2014 Subject: [Histonet] Grossing Protocol for Radical Prostates In-Reply-To: <9BAB89F57447A94A9FCFE0799B9C503015FECDF68B@EXMBXC5.crha.bewell.ca> References: , <9166D14F25C6FE418CAFA9845BE52DB9B4B9E215F8@EXMBXC2.crha.bewell.ca> <9166D14F25C6FE418CAFA9845BE52DB9B4B9E215F9@EXMBXC2.crha.bewell.ca>, <9BAB89F57447A94A9FCFE0799B9C503015FECDF68B@EXMBXC5.crha.bewell.ca> Message-ID: <9166D14F25C6FE418CAFA9845BE52DB9B4B9E215FA@EXMBXC2.crha.bewell.ca> Hi Everyone, I'm curious about what your grossing protocol is for your radical prostates. Are they submitted in total or are representative sections taken? Thanks, Leah Nichols This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From joelleweaver <@t> hotmail.com Tue May 13 17:12:00 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue May 13 17:12:04 2014 Subject: [Histonet] HER2 Message-ID: Hi Histonetters I am looking to compile a list of potential sites do send out Her2 slides for correlation on a validated assay. I have the Bond, using Refine detection. My clone is the Cb11, Oracle. Thanks in advance for any information you can provide. Joelle Weaver MAOM, HTL (ASCP) QIHC From JCBRITTON <@t> Cheshire-Med.COM Wed May 14 06:22:39 2014 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Wed May 14 06:22:51 2014 Subject: [Histonet] HER2 In-Reply-To: Message-ID: We use Integrated Oncology-Lab Corp, 521 West 57th Street, 6th floor, New York, NY 10019 800-447-5816 Josie Britton HT (ASCP) QIHC Cheshire Medical Center 580 Court Street Keene, NH 03431 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, May 13, 2014 6:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 Hi Histonetters I am looking to compile a list of potential sites do send out Her2 slides for correlation on a validated assay. I have the Bond, using Refine detection. My clone is the Cb11, Oracle. Thanks in advance for any information you can provide. Joelle Weaver MAOM, HTL (ASCP) QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From joelleweaver <@t> hotmail.com Wed May 14 06:46:15 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed May 14 06:46:19 2014 Subject: [Histonet] HER2 In-Reply-To: References: , Message-ID: Thank you Josie this is very helpful. Joelle Weaver MAOM, HTL (ASCP) QIHC > Subject: RE: [Histonet] HER2 > Date: Wed, 14 May 2014 07:22:39 -0400 > From: JCBRITTON@Cheshire-Med.COM > To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu > > We use Integrated Oncology-Lab Corp, 521 West 57th Street, 6th floor, > New York, NY 10019 800-447-5816 > > Josie Britton HT (ASCP) QIHC > Cheshire Medical Center > 580 Court Street > Keene, NH 03431 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle > weaver > Sent: Tuesday, May 13, 2014 6:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HER2 > > Hi Histonetters > I am looking to compile a list of potential sites do send out Her2 > slides for correlation on a validated assay. I have the Bond, using > Refine detection. My clone is the Cb11, Oracle. > Thanks in advance for any information you can provide. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Merry.Lewis <@t> childrens.com Wed May 14 10:57:53 2014 From: Merry.Lewis <@t> childrens.com (Merry Lewis) Date: Wed May 14 10:58:05 2014 Subject: [Histonet] Liver Core Biopsies Message-ID: Hello there! We are having some issues with our liver biopsies and can't seem to find any solutions. The biopsies are sent to us from IR already in formalin, we bake them in a 65* C oven for 30 minutes (to ensure fixation) and then we microwave process them. The microwave process is reagent alcohol, 5 mins, isopropanol, 5 mins, and finally paraffin, 2 min at 65* C and 5 min at 70* C. Worth noting: this is our procedure for all our biopsies - liver, heart, kidney, GI - and liver is the only one we are having problems with. The livers section well and don't appear to be over processed; however, without exception, they have a drying artifact on the outer edges. I have spoken to IR and they confirmed that they do not leave the biopsies out to dry for any amount of time. If they can't put them directly into formalin, the liver cores are held on saline soaked pads for no longer than a minute. I have seen suggestions about local anesthesia causing problems, but as we have no control over that and it's a necessary evil, I'd like to see if anyone has a suggestion to help minimize the artifact. Thanks! Merry Amber Lewis, HTL Children's Medical Center Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From jpiche <@t> wtbyhosp.org Wed May 14 11:01:17 2014 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Wed May 14 11:01:25 2014 Subject: [Histonet] Scale or Balance Message-ID: <631955447A364B45B9458D2905635110BA0B7329@WIN08-MBX-02.wtbyhosp.org> Hey Everyone, Does anyone know of a good brand/vendor for a balance (with a weighing dish) for the gross room? Thanks, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From joelleweaver <@t> hotmail.com Wed May 14 12:17:58 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed May 14 12:18:07 2014 Subject: [Histonet] Scale or Balance In-Reply-To: <631955447A364B45B9458D2905635110BA0B7329@WIN08-MBX-02.wtbyhosp.org> References: <631955447A364B45B9458D2905635110BA0B7329@WIN08-MBX-02.wtbyhosp.org> Message-ID: Mopec, did you try them? They make a lot of items for the gross room & morgue. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jpiche@wtbyhosp.org > To: histonet@lists.utsouthwestern.edu > Date: Wed, 14 May 2014 16:01:17 +0000 > Subject: [Histonet] Scale or Balance > > Hey Everyone, > > Does anyone know of a good brand/vendor for a balance (with a weighing dish) for the gross room? > > Thanks, > > Jessica Piche, HT(ASCP) > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed May 14 13:00:48 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed May 14 13:01:27 2014 Subject: [Histonet] Large slide rack and adaptor for Sakura stainer Message-ID: <761E2B5697F795489C8710BCC72141FF36782448@ex07.net.ucsf.edu> Hi all, Sakura has discontinued its large slide (50 x 75 mm) rack and metal adaptor for their Prisma stainer. Does anyone know a source for these that may have some left? Or do you have some you don't use anymore? The slide rack is a modified 24-slide Sakura rack that has cross bars on the bottom. The metal adaptors are inserts that slide in vertically on each end of the rack to space the slides. The catalog numbers from Sakura are: Large Slide Basket Sakura #4366 Adaptor for Large slides #4365 Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org From mw <@t> personifysearch.com Wed May 14 13:10:11 2014 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed May 14 13:10:18 2014 Subject: [Histonet] **Position Alert** New Histology Opportunity Message-ID: <0d2c01cf6f9f$c05087a0$40f196e0$@personifysearch.com> Good Morning! We have had a world leading cancer diagnostics client open a new opportunity on the West Coast (to be based in CA, OR or WA). Our client is currently searching for a talented histology professional with a strong background in the laboratory to join our clients field support team in the West. The position offers an outstanding opportunity to break into a manufacturing company, a very competitive package and excellent growth potential. Please contact me directly to learn more about the opportunity at mw@personifysearch.com or 800-875-6188 ext 103 Best Regards, Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com From mjdessoye <@t> commonwealthhealth.net Wed May 14 13:24:05 2014 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael) Date: Wed May 14 13:25:19 2014 Subject: [Histonet] Scale or Balance In-Reply-To: <631955447A364B45B9458D2905635110BA0B7329@WIN08-MBX-02.wtbyhosp.org> References: <631955447A364B45B9458D2905635110BA0B7329@WIN08-MBX-02.wtbyhosp.org> Message-ID: We have always liked Mettler-Toledo: http://www.lab-balance.com/finder/ Michael J. Dessoye, M.S.?|?Histology/Toxicology/RIA Supervisor?|?Wilkes-Barre General Hospital?|?An Affiliate of Commonwealth Health |?mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: Piche, Jessica [mailto:jpiche@wtbyhosp.org] Sent: Wednesday, May 14, 2014 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Scale or Balance Hey Everyone, Does anyone know of a good brand/vendor for a balance (with a weighing dish) for the gross room? Thanks, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From akbitting <@t> geisinger.edu Wed May 14 13:28:03 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Wed May 14 13:28:08 2014 Subject: [Histonet] RE: Large slide rack and adaptor for Sakura stainer In-Reply-To: <761E2B5697F795489C8710BCC72141FF36782448@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF36782448@ex07.net.ucsf.edu> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F679BEF4B8@GHSEXMBX1W8K1V.geisinger.edu> I posted about this earlier this year. Apparently they are no longer available in the US. I have found no substitute for these racks or adapter and Sakura was unwilling to help in any way. (Which I am still miffed about). If someone has a metal shop, here is a great opportunity. Angela K. Bitting, HT (ASCP), QIHC Technical Specialist IHC/Special Staining Geisinger Medical Center 100 N. Academy Ave. Danville, PA 17822-2300 Tel.570-214-9634 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, May 14, 2014 2:01 PM To: Histonet Subject: [Histonet] Large slide rack and adaptor for Sakura stainer Hi all, Sakura has discontinued its large slide (50 x 75 mm) rack and metal adaptor for their Prisma stainer. Does anyone know a source for these that may have some left? Or do you have some you don't use anymore? The slide rack is a modified 24-slide Sakura rack that has cross bars on the bottom. The metal adaptors are inserts that slide in vertically on each end of the rack to space the slides. The catalog numbers from Sakura are: Large Slide Basket Sakura #4366 Adaptor for Large slides #4365 Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From christina.kreutzer01 <@t> gmail.com Wed May 14 13:28:20 2014 From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer) Date: Wed May 14 13:28:22 2014 Subject: [Histonet] Luxol Fast Blue Message-ID: Hello everybody, can anybody send me a Luxol Fast Blue staining protocol for mouse/rat brain (40?m slices) and spinal cord (10?m)? Any tipps and tricks are higly appriciated!! Thank you Christina From algranth <@t> email.arizona.edu Wed May 14 14:23:58 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed May 14 14:24:03 2014 Subject: [Histonet] Luxol Fast Blue In-Reply-To: References: Message-ID: There are several procedures online. Just google Luxol fast blue staining procedures. It is an easy stain to do. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From rsrichmond <@t> gmail.com Wed May 14 19:28:15 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed May 14 19:28:19 2014 Subject: [Histonet] Re: Scale or Balance Message-ID: Jessica Piche, HT(ASCP), presumably at Waterbury Hospital in Connecticut, asks: >>Does anyone know of a good brand/vendor for a balance (with a weighing dish) for the gross room?<< You can get excellent top-loading electronic balances from Ohaus or Mettler or others, for two or three hundred dollars each. These are sold through the standard vendors like whatever Scientific Products is called this week. You must know exactly what you want, and I expect your pathologists to know this. Balance specifications change rapidly as manufacturers improve their products, so that catalog numbers won't help you much. For ordinary gross room use, you want a balance that will weigh in gram increments up to a kilogram or so, so you can weigh 10 grams of prostatic chips or 600 grams of placenta with decent precision and accuracy. For weighing parathyroid specimens, you need to be able to weigh in 10 mg increments, up to a few hundred or thousand milligrams. Weighing parathyroids is standard of care, and if your pathologists and your MBAs don't know it you need to tell them. That's going to take a second balance. (Tip - if your hospital pharmacy still does a little compounding, they may have the right balance. One of my hospitals keeps its old balance around, just for me.) Two caveats you wouldn't think of - unlike the platform balances of yore, electronic balances need replacing fairly often. And druggies will steal your balances to weigh out illegal drugs - in some venues, you have to lock your balances up when you're not using them. From baazaouinarjes <@t> gmail.com Wed May 14 20:42:50 2014 From: baazaouinarjes <@t> gmail.com (Narjes) Date: Wed May 14 20:42:58 2014 Subject: [Histonet] Dil labeling Message-ID: Hello all, Please I need some help in the lipophilic dil labeling of free floating brain sections. The mice brain were fixed in 1.5% PFA by cardia perfusion, then left one hour in the same fixative. I used the Dil labeling solution for a 200 um thick sections, the dilution was 1:150 in PBS. The sections were incubated for 48h in the Dil labeling solution then 48 in PBS in the cold room in the shaker. The problem that I had a very high background and I could not see even one spine. I need to stain only a few neurons so I can do a reasonable quantification of the size and number of the spines. Does anybody has an idea if in this case the solution or the crystal Dil is better and can I get an optimized protocol for this kind of staining. Thank you so much All the best Sent from my iPad From sccrshlly <@t> yahoo.com Wed May 14 21:03:12 2014 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Wed May 14 21:03:18 2014 Subject: [Histonet] Looking for Volunteers for NSH Career Day Message-ID: <1400119392.81756.YahooMailNeo@web162302.mail.bf1.yahoo.com> Histotechs!? Are you attending the NSH Annual Symposium in Austin this year?? We are looking for a histotech that works in research doing whole mount sections to come and present at Career Day. If you work in research and love it, we want you :)? It's a great way to share your knowledge, and you will receive CEU credits for it!? If you are interested, feel free to email me under separate cover at mcokertx@gmail.com, or even better, email the Career Day coordinator Sue Clark at dotmr@bellsouth.net. Cheers!! From rjbuesa <@t> yahoo.com Thu May 15 11:56:39 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 15 11:56:44 2014 Subject: [Histonet] Luxol Fast Blue In-Reply-To: References: Message-ID: <1400172999.60776.YahooMailNeo@web120406.mail.ne1.yahoo.com> If you are going to use Luxol Fast Blue for brain sections (its most frequent use) Goole the stain Luxol Fast Blue-Cresyl Etch Violet. This combination is much better. Ren? J.? On Wednesday, May 14, 2014 3:24 PM, "Grantham, Andrea L - (algranth)" wrote: There are several procedures online. Just google Luxol fast blue staining procedures. It is an easy stain to do. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415? ? Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MMM9XY <@t> hscmail.mcc.virginia.edu Thu May 15 12:44:17 2014 From: MMM9XY <@t> hscmail.mcc.virginia.edu (Maglione, Marilou M *HS) Date: Thu May 15 12:44:02 2014 Subject: [Histonet] IHC Platform Questions: Message-ID: Greetings Histonetters, I'm writing to ask for feedback from anyone who has used or is using the Dako Autostainers with PT Links or the Omnis. We are evaluating options and value the experience and opinions posted on this listserv. Here's a short questionnaire. Thanks in advance to all!! Dako Platform: Autostainer w/ PT link ____ Omnis _____ Previous vendor or platform: ______________________ IHC Slide volume/year: __________________________ # of instruments: _____________ Interfaced to your LIS? (Y / N) _____ 1. Implementation/conversion: Support? Easy or painful? 2. Ease of use: 3. Workflow efficiency: Did it meet expectations? Labor savings? 4. TAT: Improved? 5. Downtime: Frequency? Service response time and quality? 6. Software: Issues? Ease of use? 7. Slide Quality: Fair, Good, Excellent? Repeat rate? 8. Cost/test: I understand if you don't want to share actual numbers. Could you say if your costs went up or down? Higher or lower than expected? 9. Vendor/platform comparison: Better or worse than ______________. Why? 10. Overall satisfaction? Comments? Feel free to email me at mmm9xy@hscmail.mcc.virginia.edu if you prefer. Thanks very much, Marilou M. Maglione Director of Anatomic Pathology University of Virginia Health System Office: 434-243-8459 Cell: 434-465-3327 e-mail: mmm9xy@hscmail.mcc.virginia.edu From christina.kreutzer01 <@t> gmail.com Fri May 16 04:37:41 2014 From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer) Date: Fri May 16 04:37:45 2014 Subject: [Histonet] Luxol Fast Blue In-Reply-To: <1400172999.60776.YahooMailNeo@web120406.mail.ne1.yahoo.com> References: <1400172999.60776.YahooMailNeo@web120406.mail.ne1.yahoo.com> Message-ID: Thanks everybody for the fast help!! Christina 2014-05-15 18:56 GMT+02:00 Rene J Buesa : > If you are going to use Luxol Fast Blue for brain sections (its most > frequent use) Goole the stain Luxol Fast Blue-Cresyl Etch Violet. This > combination is much better. > Ren? J. > On Wednesday, May 14, 2014 3:24 PM, "Grantham, Andrea L - (algranth)" < > algranth@email.arizona.edu> wrote: > There are several procedures online. Just google Luxol fast blue > staining procedures. It is an easy stain to do. > > Andi > > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From relia1 <@t> earthlink.net Fri May 16 07:54:56 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri May 16 07:55:06 2014 Subject: [Histonet] TGIF It's that time of year again! Message-ID: <003c01cf7106$0a630e00$1f292a00$@earthlink.net> Hello Histonetters!!, How are you? It's that time of year again so I thought I would drop you a line. What time of year? You are probably wondering. Well for the past 12 years that I have been recruiting in Pathology and Histology the beginning of the Summer is when the people you would least likely expect to be open to new opportunities are ready to make a change. Why Now? Milestone Moments for their families: i.e. graduations, weddings, grandchildren being born in other states etc. Milestone Moments at work: Favorite mentors retiring/moving on, changes due to budget cuts and reorganizations Summer Vacations: to places they have always wanted to live. It's true, not everyone makes a job change because they are unhappy. Some of the top professionals in Pathology and Histology make changes or start thinking about making changes during the summer. some of the most common reasons I have heard are listed above. They don't respond to advertisements, THEY CALL ME!! I have experienced this trend for the past 12 years. Over the next 4-8 weeks some of the best histology and pathology professionals technicians and managers across the United States could be open to new opportunities. Don't Miss Out! If you have an immediate opening let's talk ASAP. You can reach me toll free at 866-607-3542 or on my cell at 407-353-5070 or via email at relia1@earthlink.net If you anticipate a need in the near future, let's talk. Most of these candidates are pretty flexible with their lead time and can adjust start dates to accommodate you. If you have something coming up a little later on, please shoot me an email and let me know because some of them even have a little longer lead time which may work for you. Have a Great Day!!! Thanks-Pam President - RELIA Solutions 866-607-3542 relia1@earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From lindamargraf <@t> gmail.com Fri May 16 08:42:47 2014 From: lindamargraf <@t> gmail.com (Linda Margraf) Date: Fri May 16 08:42:54 2014 Subject: [Histonet] Fwd: Help in Dil labeling of brain sections References: Message-ID: <26083915-8535-4BAD-B024-19CD3A4B846F@gmail.com> Sent from my iPhone Begin forwarded message: > From: narjes baazaoui > Date: May 14, 2014 at 1:35:04 PM CDT > To: histonet-owner@lists.utsouthwestern.edu > Subject: Help in Dil labeling of brain sections > > Hello all, > Please I need some help in the lipophilic dil labeling of free floating brain sections. The mice brain were fixed in 1.5% PFA by cardia perfusion, then left one hour in the same fixative. I used the Dil labeling solution for a 200 um thick sections, the dilution was 1:150 in PBS. The sections were incubated for 48h in the Dil labeling solution then 48 in PBS in the cold room in the shaker. The problem that I had a very high background and I could not see even one spine. I need to stain only a few neurons so I can do a reasonable quantification of the size and number of the spines. Does anybody has an idea if in this case the solution or the crystal Dil is better and can I get an optimized protocol for this kind of staining. > Thank you so much > All the best > Narjes > > -- > Narjes Baazaoui, PhD student > Institute of Basic research (IBR), > Laboratory of chemical neuropathology > 3476715743 From portera <@t> msu.edu Fri May 16 08:51:20 2014 From: portera <@t> msu.edu (Amy Porter) Date: Fri May 16 08:51:29 2014 Subject: [Histonet] Looking for assistance with Osteo-Bed Bone MM Message-ID: <001a01cf710d$eb1e78b0$c15b6a10$@edu> Anyone out there using this product for undecalcified bone sections in rodents - looking for information to potentially start this process in our lab. Thanks - amy Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory portera@msu.edu From bfoster <@t> mme1.com Fri May 16 12:55:52 2014 From: bfoster <@t> mme1.com (Barbara Foster) Date: Fri May 16 12:56:10 2014 Subject: [Histonet] What are you using to prepare images for reports? Message-ID: <201405161756.s4GHu25I011043@atl4mhob03.myregisteredsite.com> Hi, We've been having a lot of discussions about doing a better job of preparing color images for publication in reports and presentations. What are you all using? (Nothing... Photoshop... something else?) Thanks, Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com *A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277 From tkngflght <@t> yahoo.com Fri May 16 13:40:43 2014 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri May 16 13:40:50 2014 Subject: [Histonet] Adenomatous pituitary (spelling?) Message-ID: <1400265643.45033.YahooMailNeo@web161206.mail.bf1.yahoo.com> Help? ?We're contributing to a breast research case and need ample supply of adenomatous pituitary. Anyone have historical cases or a source we might obtain FFPE blocks or slides? Appreciate the help! ? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT?Tech at a time.? 281.852.9457?Office 800.756.3309?Phone & Fax? admin@fullstaff.org Sign up for the FREE?newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please?include your name and specialty in the body of the email. From esulkosky <@t> gmail.com Fri May 16 16:14:46 2014 From: esulkosky <@t> gmail.com (Eric Sulkosky) Date: Fri May 16 16:14:58 2014 Subject: [Histonet] Digital Pathology/ Telepathology Message-ID: Hello Histonetter's, I hope this email finds you all well. I am currently working on a project regarding the general acceptance or non acceptance of Digital Pathology (DP)/ Telepathology within the workplace. I need your help to gather as much information as possible. The more information I receive the better, good or bad. I would like to know what your thoughts/ opinions/ needs and concerns are regarding the general acceptance of DP into your daily workflow. All labs are welcome to comment (Hospital, Research, Government, Reference, Physician Office, Research and Veterinary) Pathology practices to include Histologists, Pathologists, Administration, Risk Management and Patient Safety departments. This discussion is open to the US and the International communities. My areas of Interest are the following: Research Clinical Peer Review Consultation Tumor Board Cancer Registry Do you currently utilize a DP/Telepathology system or plan to implement one in the near future? if so, what vendor and why? What are the pro's and con's of the system or systems you are currently utilizing and in what setting? Are you utilizing DP for image analysis? If so, what IHC, ISH, CISH/ SISH or FISH tests are you currently performing? Does you lab lab plan on adding additional tests? If not, do you have any plans on implementing one in the near future? Why? Does IVD or FDA clearance effect your decision in utilizing a DP system? Do you consider DP/Telepathology as a helpful resource or hinderance on your workflow? Why? What can be done to improve current DP systems in use? What are the most important factors or benefits for you or your lab to utilize or not utilize in DP? Image analysis Workflow Speed Accuracy Throughput Reimbursement Software Digital App LIS/EMR integration Server vs. Web based Validation CAP/ Regulatory Agencies Satellite labs Frozen sections What has been your past or present experiences with DP/Telepathology? What medical value do you think DP/ Telepathology brings or does not bring to the pathology profession and why? Is your lab resistant to change or to the adoption of emerging technologies ? If so, why? Areas to think about: How much time is spent by your lab pulling slides for send out, tumor board and research? How much does your lab spend on send outs (Fedex, UPS, or DHL) annually? What is the average TAT for consultations utilizing manual send outs for diagnosis? What percent of slides are lost, misplaced or never returned to rightful institutions? This post is open for discussion and all comments are welcome. Feel free to post your comments to the histonet or contact me offline. I look forward to hearing from you soon. Best regards, Eric Sulkosky esulkosky@gmail.com From amosbrooks <@t> gmail.com Sat May 17 07:19:26 2014 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat May 17 07:19:41 2014 Subject: [Histonet] Re: Histonet Digest, Vol 126, Issue 15 In-Reply-To: <5374f2a6.8361b60a.5a7a.0847SMTPIN_ADDED_MISSING@mx.google.com> References: <5374f2a6.8361b60a.5a7a.0847SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi Andi, I have a Google Doc of our procedure. I just sent you an invite to view it. If anyone else wants to see it, just let me know I'll share :-) I know the procedure calls for overnight in the LFB solution. It's for the best, the results are much better if you are patient. It is really important to not run the rehydration to water. End in 95% ETOH, the water will really kill the stain. Good luck, Amos On Thu, May 15, 2014 at 1:00 PM, wrote: > Message: 7 > Date: Wed, 14 May 2014 19:23:58 +0000 > From: "Grantham, Andrea L - (algranth)" > Subject: Re: [Histonet] Luxol Fast Blue > To: Christina Kreutzer > Cc: "" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > There are several procedures online. Just google Luxol fast blue staining > procedures. It is an easy stain to do. > > Andi > From gloria.cole <@t> usa.net Sat May 17 12:23:19 2014 From: gloria.cole <@t> usa.net (Gloria Cole) Date: Sat May 17 12:23:34 2014 Subject: [Histonet] time frame on a CLIA application in colorado Message-ID: <00fd01cf71f4$b3202160$19606420$@usa.net> Hello, I have been striking out on getting the local CLIA office to call me back I regards to some information. I am turning to you all in hopes someone in the Denver Colorado area can help me. I need to find out the time frame it takes from the time a CLIA application (high complexity lab and assuming all information is filled out correctly) is submitted to the time a coupon for payment is received in the mail? 3 months? 6 months? If anyone can help me find this information, I would appreciate it. Thanks, Gloria From ruio7 <@t> hotmail.com Sat May 17 17:39:06 2014 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Sat May 17 17:39:09 2014 Subject: [Histonet] Help for identifying the blue stained structure Message-ID: Hello, I have an embryonic sample that decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This is a cranial region where the bone is being resorbed, so I expected to see the bone (dark blue in trabecular), and red blood, and adjacent white spaces that is being resorbed. Instead, there is a very uniform, granular structure stained with blue without any nuclei at the resorbed regions. This structure looks like almost crystal or some kind of secretion leakage from the ossifying bone. I need a help to identify this blue stained things. I don?t think this is osteoid, because at later stage embryos, there is no bone at this region. If you have some suggestions for other stain to identify this blue thing, or help identifying this, I really appreciate it. Thank you in advance, Rui From mhanna <@t> histosearch.com Sun May 18 16:47:21 2014 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Sun May 18 16:47:27 2014 Subject: [Histonet] Help for identifying the blue stained structure In-Reply-To: References: Message-ID: <53792A69.9010405@histosearch.com> Hi Rui, You uploaded a tif image and a number of browsers don't support tif images. Jpeg, gif and png images are the best image formats to use because they are universally supported. I converted your image to a jpeg image and posted it at: http://histosearch.com/imageupload/help-for-identifying-the-blue-stained-structure-jpeg/ Best Regards, Marvin Hanna On 05/17/2014 06:39 PM, Rui TAHARA wrote: > > Hello, > I have an embryonic sample that > decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline > Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This > is a cranial region where the bone is being resorbed, so I expected to see the > bone (dark blue in trabecular), and red blood, and adjacent white spaces that > is being resorbed. Instead, there is a very uniform, granular structure stained > with blue without any nuclei at the resorbed regions. This structure looks like > almost crystal or some kind of secretion leakage from the ossifying bone. I > need a help to identify this blue stained things. I don't think this is osteoid, > because at later stage embryos, there is no bone at this region. > > If you have some suggestions for other > stain to identify this blue thing, or help identifying this, I really > appreciate it. > > > > Thank you in advance, > > > > Rui > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruio7 <@t> hotmail.com Sun May 18 18:08:12 2014 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Sun May 18 18:08:21 2014 Subject: [Histonet] Help for identifying the blue stained structure In-Reply-To: <53792A69.9010405@histosearch.com> References: , <53792A69.9010405@histosearch.com> Message-ID: Hi, Thank you so much. I was wondering why the image has not been seen on the site, and thought its been processed. Thank you for uploading the image. Rui Date: Sun, 18 May 2014 17:47:21 -0400 From: mhanna@histosearch.com To: ruio7@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help for identifying the blue stained structure Hi Rui, You uploaded a tif image and a number of browsers don't support tif images. Jpeg, gif and png images are the best image formats to use because they are universally supported. I converted your image to a jpeg image and posted it at: http://histosearch.com/imageupload/help-for-identifying-the-blue-stained-structure-jpeg/ Best Regards, Marvin Hanna On 05/17/2014 06:39 PM, Rui TAHARA wrote: Hello, I have an embryonic sample that decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This is a cranial region where the bone is being resorbed, so I expected to see the bone (dark blue in trabecular), and red blood, and adjacent white spaces that is being resorbed. Instead, there is a very uniform, granular structure stained with blue without any nuclei at the resorbed regions. This structure looks like almost crystal or some kind of secretion leakage from the ossifying bone. I need a help to identify this blue stained things. I don?t think this is osteoid, because at later stage embryos, there is no bone at this region. If you have some suggestions for other stain to identify this blue thing, or help identifying this, I really appreciate it. Thank you in advance, Rui _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah.Dysart <@t> stdavids.com Mon May 19 10:59:37 2014 From: Sarah.Dysart <@t> stdavids.com (Sarah.Dysart@stdavids.com) Date: Mon May 19 10:59:46 2014 Subject: [Histonet] Billing Message-ID: <34C22BB94729434598D767D3F4EB95E0BD26786060@FWDCWPMSGCMS03.hca.corpad.net> When it comes to the medicare codes...question... So you have one specimen that has 10 blocks. AE1/AE3 is ordered on all 10 blocks. Can you bill AE1/AE3 1st Antibody once (G0461), then AE1/AE3 Additional (G0462) nine times? Thanks, Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 From Joyce.Weems <@t> emoryhealthcare.org Mon May 19 11:20:36 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon May 19 11:20:44 2014 Subject: [Histonet] RE: Billing In-Reply-To: <34C22BB94729434598D767D3F4EB95E0BD26786060@FWDCWPMSGCMS03.hca.corpad.net> References: <34C22BB94729434598D767D3F4EB95E0BD26786060@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: No - one G0461. If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1 and G0462 x 2. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com Sent: Monday, May 19, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing When it comes to the medicare codes...question... So you have one specimen that has 10 blocks. AE1/AE3 is ordered on all 10 blocks. Can you bill AE1/AE3 1st Antibody once (G0461), then AE1/AE3 Additional (G0462) nine times? Thanks, Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From bcooper <@t> chla.usc.edu Mon May 19 11:27:39 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Mon May 19 11:27:46 2014 Subject: [Histonet] BRAF (V600E) on BondMax Message-ID: Hey Histonetters, Has anyone successfully worked up Spring Bioscience's BRAF (v600e) on the Leica Bond? We've experimented with different pretreatment options and serially diluted the heck out this antibody (up to 1:6,400) and we're still getting background and unexpected staining. Our pathologists are thusfar "unconvinced." Any help would be greatly appreciated . . . Thank you! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From azdudley <@t> hotmail.com Mon May 19 11:30:58 2014 From: azdudley <@t> hotmail.com (anita) Date: Mon May 19 11:31:01 2014 Subject: [Histonet] new processor Message-ID: Looking for a new processor, we have a VIP now that is old, what brands do others like? Thanks , Anita Providence Hosp Mobile, Al. From cecystan76 <@t> gmail.com Mon May 19 12:32:36 2014 From: cecystan76 <@t> gmail.com (Cecy Stan) Date: Mon May 19 12:32:43 2014 Subject: [Histonet] HTL certification Message-ID: Hello everyone, I'm starting to prepare for my HTL certification (I am very nervous and anxious but also very excited about this decision to go for it). I was just curious to know how you guys prepared for it, and how long it took for you to prepare before taking the test. Will 6 months preparation be enough? (I know that may depend on the individual; it's just that I had my Masters over 10 years ago and I haven't studied this much since then). I have Freida L. Carson's 3rd Edition book -- quite daunting to memorize -- but the outline ASCP has provided for study seems to be helpful. Do you have any other book/study aid suggestions? Thank you in advance for your input and advice! C.A. From epeters2 <@t> gmu.edu Mon May 19 12:46:47 2014 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Mon May 19 12:46:54 2014 Subject: [Histonet] HTL certification In-Reply-To: References: Message-ID: <1400521609188.54980@gmu.edu> Hi C.A., I don't have the HT or HTL, but from my college teaching experience in histology and histotechniques, I just wanted to caution you that memorizing is not what you should be doing. You need to understand concepts, so that when you need to troubleshoot problems you will be able to think through things, rule some things out, and make sense of the situation. I see this all the time with my students, they forget things they memorize, but then they finally understand things and can figure things out. One of the new teaching tools is having students prepare "concept maps," to see the relationships of topics and terms, and these linkages will help you in the long run. For histology examples, see: http://www.biologycorner.com/anatomy/tissues/tissue_concept_map_samples.html I don't know of any concept maps for histotechnology on the web, but I am going to add this to my course next year! Esther Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cecy Stan Sent: Monday, May 19, 2014 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL certification Hello everyone, I'm starting to prepare for my HTL certification (I am very nervous and anxious but also very excited about this decision to go for it). I was just curious to know how you guys prepared for it, and how long it took for you to prepare before taking the test. Will 6 months preparation be enough? (I know that may depend on the individual; it's just that I had my Masters over 10 years ago and I haven't studied this much since then). I have Freida L. Carson's 3rd Edition book -- quite daunting to memorize -- but the outline ASCP has provided for study seems to be helpful. Do you have any other book/study aid suggestions? Thank you in advance for your input and advice! C.A. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> skm.org.pk Tue May 20 07:09:28 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Tue May 20 07:07:38 2014 Subject: [Histonet] positive control indoleamine 2-3 dioxygenase Message-ID: Hi All, We are going to optimize indoleamine 2-3 dioxygenase (IDO1) expression in breast cancer tissue by immunohistochemistry on formalin fixed paraffin sections. I shall be thankful if any one please let me know about the "positive control. Regards Muhammad Tahseen Sr.Supervisor Histopathology SKMCH&RC Lahore Pakistan From Michael.LaFriniere <@t> ccplab.com Tue May 20 08:03:55 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Tue May 20 08:04:07 2014 Subject: [Histonet] immunohistochemisty coding In-Reply-To: References: <34C22BB94729434598D767D3F4EB95E0BD26786060@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29670A90@AHCMSASEXCH02.my.ahc.local> Hi Sarah, Joyce is correct, under medicare changes in pathology coding, you can not bill for each of the blocks of the same antibody applications, only one charge per specific antibody mentioned in the pathology report is allowable. Medicare immuno billing is per "specimen" only for each specific antibody used, example if you used s100 for the same specimen (wide melanoma excision)on 15 different blocks, under medicare you can only bill one GO461, yes- not fair when the need is appropriate, I must voice my opinio)- this probably will lower some of the over utilization and inappropriate billing practices that many of us have seen in the immunohistochemistry world and the labs that have capitalized on the practice, the problem with the lower reimbursements and change in medicare coding hurts many of our labs who have used the utilization of immunohistochemistry appropriately. However, "under current 2014" CPT coding on non medicare can you bill for the additional work per block using the 88342 and 88343 cpt code, I am sure moving forward we are going to continue to see adjustments to the pathology coding processes! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 19, 2014 12:21 PM To: 'Sarah.Dysart@stdavids.com'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Billing No - one G0461. If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1 and G0462 x 2. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com Sent: Monday, May 19, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing When it comes to the medicare codes...question... So you have one specimen that has 10 blocks. AE1/AE3 is ordered on all 10 blocks. Can you bill AE1/AE3 1st Antibody once (G0461), then AE1/AE3 Additional (G0462) nine times? Thanks, Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Tue May 20 09:48:20 2014 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue May 20 09:48:27 2014 Subject: [Histonet] antibody vials Message-ID: Does anyone have any good ideas for keeping the little vials of concentrated antibodies neat and organized in the fridge? Right now we keep ours in empty pipette tip boxes (without tip holders) but they're always falling over and mixed up. We use several different vendors for our concentrated antibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From murphyv <@t> karmanos.org Tue May 20 09:59:21 2014 From: murphyv <@t> karmanos.org (Murphy, Valerie) Date: Tue May 20 09:59:31 2014 Subject: [Histonet] RE: antibody vials In-Reply-To: References: Message-ID: I use cardboard cryovial boxes and they work quite well. You can remove some of the dividers to make space for the larger vials. http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Freezer-Racks/Cryovial-Boxes-Cardboard/ Valerie Ratliff B.Sc HTL(ASCP) Research Assistant Department of Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: murphyv@karmanos.org Better treatments. Better outcomes. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Clare Thornton [CThornton@dahlchase.com] Sent: Tuesday, May 20, 2014 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody vials Does anyone have any good ideas for keeping the little vials of concentrated antibodies neat and organized in the fridge? Right now we keep ours in empty pipette tip boxes (without tip holders) but they're always falling over and mixed up. We use several different vendors for our concentrated antibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. From dakshnapriya <@t> neo.tamu.edu Tue May 20 10:23:27 2014 From: dakshnapriya <@t> neo.tamu.edu (Balasubbramanian, Dakshnapriya) Date: Tue May 20 10:23:30 2014 Subject: [Histonet] HELP- Cryosectioning FAT! Message-ID: <211285224.17352091.1400599407431.JavaMail.root@neo.tamu.edu> Hi all, I've been having problems with cryosectioning fatty tissue for a long time now. The section leaves a hole in the middle. I know this is probably a much discussed topic, but I've tried a lot of strategies with no luck. The tissue is of mouse origin and has micro-lesions buried inside fat. So the fat needs to be cut in order to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tried rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -80C. Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna From azdudley <@t> hotmail.com Tue May 20 10:26:13 2014 From: azdudley <@t> hotmail.com (anita) Date: Tue May 20 10:26:17 2014 Subject: [Histonet] new processor Message-ID: Thanks everyone for your input on processors, I think we will stay with the VIP, it has been a good machine just getting older now. everyone have a great day!!!! Anita Dudley Providence Hosp Mobile, Alabama 36695 From amurvosh <@t> advancederm.net Tue May 20 10:29:05 2014 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Tue May 20 10:29:15 2014 Subject: [Histonet] antibody vials In-Reply-To: References: Message-ID: <4AD6A4E531E8C943A730559B6B81DF07DA8970@dc.Advancederm.net> We got some plexiglass dividers at the "Container Store" if you have one near or check online. Can't remember what they were called but they had different sized square compartments that fit various venders antibodies. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Tuesday, May 20, 2014 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody vials Does anyone have any good ideas for keeping the little vials of concentrated antibodies neat and organized in the fridge? Right now we keep ours in empty pipette tip boxes (without tip holders) but they're always falling over and mixed up. We use several different vendors for our concentrated antibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dakshnapriya <@t> neo.tamu.edu Tue May 20 10:59:16 2014 From: dakshnapriya <@t> neo.tamu.edu (Balasubbramanian, Dakshnapriya) Date: Tue May 20 10:59:26 2014 Subject: [Histonet] HELP- Cryosectioning FAT! In-Reply-To: <38C4D4E967EA18499D3B8E80E0A32923938158D0@TX1P03DAG0302.apptixhealth.net> Message-ID: <601376475.17568918.1400601556617.JavaMail.root@neo.tamu.edu> Thanks Sandra! My next step was to try that. Dakshna ----- Original Message ----- From: "Sandra E. Esparza" To: "Dakshnapriya Balasubbramanian" Sent: Tuesday, May 20, 2014 10:34:25 AM Subject: RE: [Histonet] HELP- Cryosectioning FAT! You might want to dry Liquid Nitrogen on the face of the block before you cut it. Sandra Sandra Esparza HT (ASCP), QIHC Histotechnologist Mohs Austin Dermatologic Surgery Center 512-324-7468 x84027 sesparza@seton.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Dakshnapriya Sent: Tuesday, May 20, 2014 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP- Cryosectioning FAT! Hi all, I've been having problems with cryosectioning fatty tissue for a long time now. The section leaves a hole in the middle. I know this is probably a much discussed topic, but I've tried a lot of strategies with no luck. The tissue is of mouse origin and has micro-lesions buried inside fat. So the fat needs to be cut in order to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tried rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -80C. Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From dakshnapriya <@t> neo.tamu.edu Tue May 20 11:03:08 2014 From: dakshnapriya <@t> neo.tamu.edu (Balasubbramanian, Dakshnapriya) Date: Tue May 20 11:03:12 2014 Subject: [Histonet] HELP- Cryosectioning FAT! In-Reply-To: <7578207839F50248A7A6CD33517295EA4F1514C2@MCL-EXMB03.mfldclin.org> Message-ID: <262036458.17595765.1400601788819.JavaMail.root@neo.tamu.edu> Hi Laurie, No, the tissue hasn't been fixed before freezing. My prof doesn't prefer the method, but if nothing else works, we might give it a try. Any suggested protocols for fixation? Thanks! Dakshna ----- Original Message ----- From: "Laurie J King" To: "Dakshnapriya Balasubbramanian" Sent: Tuesday, May 20, 2014 10:37:35 AM Subject: RE: [Histonet] HELP- Cryosectioning FAT! Dakshna, Has this tissue been fixed before freezing by any chance? laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Dakshnapriya Sent: Tuesday, May 20, 2014 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP- Cryosectioning FAT! Hi all, I've been having problems with cryosectioning fatty tissue for a long time now. The section leaves a hole in the middle. I know this is probably a much discussed topic, but I've tried a lot of strategies with no luck. The tissue is of mouse origin and has micro-lesions buried inside fat. So the fat needs to be cut in order to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tried rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -80C. Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From dgonzalez <@t> cellmarque.com Tue May 20 15:33:17 2014 From: dgonzalez <@t> cellmarque.com (Daniel Gonzalez) Date: Tue May 20 15:34:28 2014 Subject: [Histonet] RE: Pankeratin- Trouble with Mast Cell Staining In-Reply-To: References: Message-ID: <5CFB864946430E42984B4F78B3BDE5F1A9BD3312@CMROCMB1.cellmarque.local> Posting for my colleague, Jeff Gordon, because his post did not go through successfully: "Suzie, your histonet post was forwarded to me by a colleague, and I thought I could help with your mast cell staining dilemma with pan-keratin. University of Pennsylvania published an article a while back about "undesirable" reactivity of cytokeratins that focused primarily on sentinel lymph node testing. In their study they found that when cytokeratin markers OTHER than strictly AE1/AE3 (with no other components) were used, other non-epithelial elements were being labeled that they deemed "undesirable." This entire article can be found here for your's and your pathologists' reference: http://www.archivesofpathology.org/doi/pdf/10.1043/0003-9985(2000)124%3C1310%3AUCIONN%3E2.0.CO%3B2 Based on these findings, and because another person in the thread also indicated that they may be dealing with the same issue, cervical tissue that was rich in mast cells was stained on a Ventana Ultra with cytokeratin AE1/AE3 ONLY as well as with cytokeratin OSCAR (which is another pan-keratin but with a lesser sensitivity to squamous epithelium than AE1/AE3) with CC1 mild heat retrieval and 16 minute heated primary incubation and Ultraview detection. To confirm the presence of mast cells in the tissue, both CD117 and tryptase were also stained on the same tissue with the same protocols. The results showed that though there was an abundance of mast cells present labeled by tryptase and CD117, there was no detectable staining of mast cells with either cytokeratin AE1/AE3 or with cytokeratin OSCAR, while the epithelial portion of the tissue stained with both cytokeratins (though the OSCAR was noticeably weaker because of the lower sensitivity to squamous epithelium). The stains can be seen here: http://www.cellmarque.com/cmc/AO_mastcellreactivity.php Hopefully this helps. If you have any questions, please contact us at your convenience." Thank you -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miller, Suzie Sent: Tuesday, May 06, 2014 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pankeratin- Trouble with Mast Cell Staining Hello Everyone, If you have a Ventana Ultra and use (Ventana# 760-2595) Pankeratin AE1/AE3/PCK29, would you consider sharing your staining protocol? Our Pathologists are displeased with the amount of mast cell staining with our current protocol so we are looking to explore other protocols or suggestions. Thank you for your assistance, Suzie Suzie Miller, MLT ASCP Senior Histotech Mercy Health System of Maine Laboratory millers@emhs.org ------------------------------------------------------------------------------------------------------------------- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHEILA.HERRINGTON <@t> interiorhealth.ca Tue May 20 16:47:52 2014 From: SHEILA.HERRINGTON <@t> interiorhealth.ca (HERRINGTON, SHEILA) Date: Tue May 20 16:48:06 2014 Subject: [Histonet] RE: Pankeratin- Trouble with Mast Cell Staining In-Reply-To: <5CFB864946430E42984B4F78B3BDE5F1A9BD3312@CMROCMB1.cellmarque.local> References: <5CFB864946430E42984B4F78B3BDE5F1A9BD3312@CMROCMB1.cellmarque.local> Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06E9BC964B@DC1SERV352.interiorhealth.ca> We found that if using the stronger proteases/enzymes for retrievals, there tended to be more mast cell staining seen. Sheila Herrington Technical Lead - Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daniel Gonzalez Sent: Tuesday, May 20, 2014 1:33 PM To: 'Miller, Suzie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pankeratin- Trouble with Mast Cell Staining Posting for my colleague, Jeff Gordon, because his post did not go through successfully: "Suzie, your histonet post was forwarded to me by a colleague, and I thought I could help with your mast cell staining dilemma with pan-keratin. University of Pennsylvania published an article a while back about "undesirable" reactivity of cytokeratins that focused primarily on sentinel lymph node testing. In their study they found that when cytokeratin markers OTHER than strictly AE1/AE3 (with no other components) were used, other non-epithelial elements were being labeled that they deemed "undesirable." This entire article can be found here for your's and your pathologists' reference: http://www.archivesofpathology.org/doi/pdf/10.1043/0003-9985(2000)124%3C1310%3AUCIONN%3E2.0.CO%3B2 Based on these findings, and because another person in the thread also indicated that they may be dealing with the same issue, cervical tissue that was rich in mast cells was stained on a Ventana Ultra with cytokeratin AE1/AE3 ONLY as well as with cytokeratin OSCAR (which is another pan-keratin but with a lesser sensitivity to squamous epithelium than AE1/AE3) with CC1 mild heat retrieval and 16 minute heated primary incubation and Ultraview detection. To confirm the presence of mast cells in the tissue, both CD117 and tryptase were also stained on the same tissue with the same protocols. The results showed that though there was an abundance of mast cells present labeled by tryptase and CD117, there was no detectable staining of mast cells with either cytokeratin AE1/AE3 or with cytokeratin OSCAR, while the epithelial portion of the tissue stained with both cytokeratins (though the OSCAR was noticeably weaker because of the lower sensitivity to squamous epithelium). The stains can be seen here: http://www.cellmarque.com/cmc/AO_mastcellreactivity.php Hopefully this helps. If you have any questions, please contact us at your convenience." Thank you -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Miller, Suzie Sent: Tuesday, May 06, 2014 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pankeratin- Trouble with Mast Cell Staining Hello Everyone, If you have a Ventana Ultra and use (Ventana# 760-2595) Pankeratin AE1/AE3/PCK29, would you consider sharing your staining protocol? Our Pathologists are displeased with the amount of mast cell staining with our current protocol so we are looking to explore other protocols or suggestions. Thank you for your assistance, Suzie Suzie Miller, MLT ASCP Senior Histotech Mercy Health System of Maine Laboratory millers@emhs.org ------------------------------------------------------------------------------------------------------------------- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madary <@t> verizon.net Wed May 21 05:54:10 2014 From: madary <@t> verizon.net (madary@verizon.net) Date: Wed May 21 05:54:24 2014 Subject: [Histonet] HTL Message-ID: <11682425.1656319.1400669650887.JavaMail.root@vznit170170> &nb cover to cover to cover. Nick(Rocky) Madar Joni Madary, PhD(in life) < On 05/20/14, hist Send Histonet [1]histonet@lists.utsout To subscribe or unsubscribe via the World Wide [2]http://lists.utsouthwe or, via email, send a message [3]histonet-r You can reach the person man [4]histonet-owner@lists.utso When replying, please edit your Subject line than "Re: Contents of Histonet digest...&q Today's Topics: 1. HTL certification (C 2. RE: HTL certification (Esther C Peters) 3. pos (Pathology-Histology S 4. immunohistochemisty coding (Michael LaFriniere) 5. antibody vials (Clare Thornton) 6. RE: antibody vials (Mu 7. HELP- Cryosectioning FAT! (Balasubbramanian, Daks 8. new processor (anita) 9. RE: antibody vials (A 10. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Da 11. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Dak ----------------------------------------------- ----------------------- Message: 1 Date: Mon, 19 May 2014 1 From: Cecy Stan <[5]cecystan76@gmail.com> Subject: [Histonet] HTL certification To: [6]histone Message-ID: , <[11]histonet@lists.utsouthwestern.edu> Me Content- Hi C.A., I don't have the HT or HTL, but from my college teaching experience in histology and histotechniques, I just wanted to caution you that memoriz concepts, so th able to think through the situation. I see this things they memorize, but then t can figure things out. One of the new tea students prepare "concept maps," to see the and terms, and these linkages will help you in the histology examples, see: [13]http://www.biologycorner.com/anatomy/tissues/tissue_conce pt_map_samples.html I don't know of any concept maps for his I am going to add this to my course next year! Esther Esther C. Peters, Ph.D. Assistant Profes Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: D Phone: 703-993-3462 Fax: 703-993-1066e-mail: [14]epeters2@gmu.edu [15]https://bluprd0511.outlook.com/owa/redir.aspx?C =ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKr rDjhw ______________ From: [16]histonet-bo ounces@lists.utsouthwestern.edu> on behalf of Cecy Stan <[18]cec ystan76@gmail.com> Sent: Monday, May 19, 2014 1:32 PM To: Subject: [Hist Hello everyone, I'm starting and anxious but I was just it took fo preparation be I ha since t I have Freida L. Carson's 3rd Edition book -- quite daunti memorize -- but the outline ASCP has provided for study seems t you have any other book/study aid suggestions? < C.A. _______ Histonet mailing list [20]Histonet@lists.utsouthwestern.edu [21]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Messa Date: Tue, 20 May 2014 12:09:28 +0000 From: "Pathology Subject: To: "[23]histonet@lists.utsouthwestern.edu" <[24]histonet@lists.utsouthwestern.edu> Cc: "[25]tahseen0009@gmail.com" <[26]tahseen0009@gmail.com>Message-ID: , "[30]histonet@lists.utsou <[31]histonet@lists.utsout Message-ID: <4A2A16B9707CE04E9C[32]B6C82DC18C1D29670A90@AHCMSASEXCH02.my.ahc.lo cal> Content-Type: text/plain; charset="us-ascii" Hi Sarah, Joyce is correct, under medicare changes in patho can not bill for each of the blocks of the same antibody a pplications, only one charge per specific antibody mentioned in the patholo "specimen" s100 for the sam blocks, under medicare y when the need is appropriate, I must voice my opinio)- this probably will lower some of the over utilization and inappropriate billing practices that many of us have seen capitalized on the reimbursements and change in medicare who have used the utilization of immunohisto However, "under current 2014& you bill for the additional work per b cpt code, I am sure moving forward we are go adjustments to the pathology coding processes! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suit P: 240.471.3427 * F: 240.471.3401 * Ce [33]michael.lafriniere@CCPLab.com -----Original Message----- From: [34]histonet-bounces@lists.utsouthwestern.edu [ma ilto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joy Sent: Monday, May 19, 2014 12:21 PM To: '[35]Sarah.Dy [36]histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Billing No - one G0461. < and G0462 Joyce Weems Pathology Manager 678-843-7376 Pho 678-843-7831 Fax [37]joyce.weems@emoryhealthcare.o [38]www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-ma Joseph's Hospital an recipient(s). It may contai confidential. Any unauthorized review distribution is prohibited. If you are not the intend please delete this message, and reply to the sender regarding error in a separate email. -----Original Message----- [[40]mailto:histonet-bounces@lists.utsouthwestern.e [41]Sarah.Dysart@stdavids.com Sent: Mon To: [42]histonet@lists.utsout Subject: [Histonet] Billing When it comes So you have one specimen tha AE1/AE3 is ordered on all 10 blocks. Can you bil Additional (G0462) nine t Thanks, Sarah E. Dysart, BA, HT (ASCP), QIHC (A David's North Austin Medical Center 1222 Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing [43]Histonet@lists.utsouthwestern.edu [44]http://lists.utsouthwestern.edu/mailman/list ________________________________ Th of t privileged i recipien distribution or cop prohibite If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the or ________________________ Histonet mailing list [45]H [46]http://lists.utsouthwestern.edu/mailman/listinfo/histonet < ------------------------------ Message: 5 Date: From: Clare Thornton <[47]CThor Subject: [Histonet] antibody vials To: Mes Content-Type: text/plain; c Does anyone have any good ideas fo concentrated antibodies neat and organized in we keep ours in empty pipette tip boxes (without ti they're always falling over and mixed up. We use several di vendors for our concentrated antibodies, so our vials are all diffe rent sizes. Thanks for any ideas! Clare J. Thornton Lead Immunohistochemistry Technologist Dahl-Chase 417 State Street, Suite 540 Bangor, ME 04401 [51]cthornton@dahlchase.com<[52]mailto:cthornton@dahl ---------------------------- Message: 6 Date: Tue, 20 May 2014 14:59:21 +0000 Fr To: Clare Thornton <[54] "histonet@l <[55]histonet@li Message-ID: Content-Type: text/plain; charset="us-ascii I use cardboard cryovial boxes and they work quite well. remove some of the dividers to make space for the larger vials. [57]http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Fre eze Valerie Research Assistant Department Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: [58]murphyv@karmanos.or Better treatments. Better outcomes. ________________________________________ From: [59]histonet-bounces@lists.utsouthwestern.edu [[60]histonet-bounces@lists.utsouthwestern.edu] on behalf of Clare Thornto Sent: Tuesday, May 20, 2014 1 To: [62]histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody vials Does anyone have any good concentrated antibodies neat and orga we keep ours in empty pipette tip boxes (wi they're always falling over and mixed up. We use se vendors for our concentrated antibodies, so our vials are a different sizes. Thanks for any ideas! Clare J. Lead Immunohistochemistry Technologist Da 417 State Street, Suite 540 Bangor, [63]cthornton@dahlchase.com<[64]mailto:cthornto __________________________________ Histonet mailing list [65]Histonet@li [66]http://li ----------- Confidentiality Notice: This email message, including any attachments , is for the sole use of the intended recipient(s) and may contain confiden intended recipient(s dissemination, unauthorized review, use of this email and any materials contained in a prohibited. If you receive this message in error, or are intended recipient(s), please immediately notify the sender by emai and destroy all copies of the original message, including attachments. ------------------------------ Message: 7Date: Tue, 20 May 2014 10:23:27 -0500 (CDT) From: "Balasubbra <[67]dakshnapriya@neo.tamu.edu>< Cryosectioning FAT! To: [68]histonet@lists.utsouthwestern.edu Message-ID: <21128 Content-Type: text/plain; charset=utf-8 Hi all, I've been having problems with cryosectioning fatty tissue for a lo ng time now. The section leaves a hole in the middle. I know this is probab with no luck. buried inside fat. So lesion. I started with a tem also at -50C; didn't work at any o rubbing the block, blade and anti-roll plate luck. I rapid-freeze the tissue with OCT at -80C. Could I try sectioning the block d done that). Any other suggestions on Any advice is much appreciated!! Dakshna ------------------------------< Message: 8 Date: Tue, 20 May 2014 10:26:13 -0500 From: Subject: [Histonet] new p To: "[71]Histonet@lists.utsouthwestern.edu <[72]histonet@lists.utsouthwestern.edu< Message-ID: Message-ID: <4A Content-Type: text/plain; charset="us-ascii" We got some plexiglass dividers at the "Container Store&q have one near or check online. Can't remember what they w had different sized square compartments that fit v antibodies. Anne -----Original Message-----< [78]histonet-bounces@lists.utsouthwestern. [[79]mailto:histonet-bounces@lists.utsout Clare Thornton Sent: Tuesday, May To: [80]histonet@lists.utsouthwestern.e Subject: [Histonet] antibody vials Does anyone have concentrated antibodies we keep ours in empty pi they're always falling over for our concentrated a Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohi Dahl-Chase Diagnostic Services 417 Stat Bangor, ME 04401 cthornton@dahlchase. _ Histonet mailing list[82]Histonet@lists.utsouthwestern.edu [83]http://lists.utsouthwestern.edu/mailman/listinfo/ ------------------------------ Message: 10 Date: Tue, 20 May 2014 10:59:16 -0500 (CDT) From: Subject: Re: [Histonet] HELP- Cryosectioning FAT! To: "Sandra E. Esparza" <[84]SEsparza@seton.org>, Message-ID: <601376475.17568918.1400601[86]556617.JavaMail.ro Content-Type: text/plain; charset=utf-8 Thanks Sandra! My next step was to try that. Dakshna ----- Original Message ----- From: "Sandra E. Esparza" To: "Dakshnapriya Balasubbram Sent: Tues Subject: RE: [Histonet] HELP- Cryosectio You might want to dry Liquid Nitrogen on the face of t before you cut it. Sandra Sandra Esparza H Histotechnologist Mohs Austin Dermatologic Surgery 512-324-7468 x84027 [89]sesparza@seton.org -----Original Message----- From: [90]his :histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Balasubbramani Sent: Tuesday, May 20, 2014 10:23 AM To: [92]histonet@lists.utsouthwestern.edu Subject: [Histonet] Hi all, I've been having pr long time now. The section le is probably a much discussed topic, strategies with no luck. The tissue is of mouse ori micro-lesions buried inside fat. So the fat needs to be cut in to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tri ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the b at -80C. Could I try sectioning the block directly from -80C? (never d Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna < Histonet mailing [93]Histonet@lists.utsouthwestern.edu [94]http://lists.utsouthwestern.edu/mailman/listi CONFIDENTIALITY NOTICE: This email m confidential and may contain p for the named recipient(s). If you are recipient(s), you are hereby notified that the disseminati distribution, and or copying of this message is strictly prohibited. If recipient(s), plea delete this email from you form immediately. Receipt by anyo recipient(s) is not a waiver of any attorney-client or other applicable privilege. ----- Message: 11 Date: Tue, 20 May 201 From: "Balasubbramanian, Dakshnapriya" <[95]dakshnapriya@neo.tamu.edu> Subject: Re: [Histon To: Laurie J King <[96]k histonet Message-ID: <262036458.175957 Hi Laurie, prefer th try. An Thanks! Dakshna ----- Original Message ----- From: "Laurie J King" << href="mailto:king.laurie@marshfieldclinic.org" ta rget="_blank">king.laurie@marshfieldclinic.org> To: "Dak Sent: Tuesday, May 20, 2014 10:37:35 AM Subject: RE: [Histo Dakshna, Has this tiss laurie - From: [99]histonet-bou [[100]mailto:histonet Balasubbramanian, Daksh Sent: Tuesday, May 20, 2014 10:23 AM To: [101]histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP- Cryo Hi all, I've been having problems wit long time now. The section leaves a hol is probably a much discussed topic, but I've t strategies with no luck. The tissue is of mouse origin and ha micro-lesions buried inside fat. So the fat needs to be cut in order to g upto -25C temperatures. I tried rubbing with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -8 done that). Any other suggestions on how to tackle this? Any ad Thanks, Dakshna _____ Histonet mailing list [103]http://lists.utsouthwestern.edu/mailman/listinfo/histon _________________________________________________________ _____________ The contents of this message may contain private, protec privileged information. If you received this message in error, should destroy the e-mail message and any attachments or copies, and yo any info advise of the erro Thank you for your cooperati ------------------------------ ______ Histonet mailing list < href="mailto:Histonet@lists.utsouthwestern.edu" t arget="_blank">Histonet@lists.utsouthwestern.edu [104]http://lists.utsouthwestern.edu/mailman/listinfo/histone End of Histonet Digest, Vol 126, Issue 20 *********** References 1. file://localhost/tmp/3D"mailto 2. 3D"http://lists.utsouthwest=/ 3. file://localhost/tmp/3D"mai 4. 3D"mailto:histonet- 5. file://localhost/tmp/3D"mai 6. 3D"mailto:histonet@lists.utsouthwestern.edu" 7. 3D"mailto:ApGujjkoEAgjAzA 8. 3D"mailto:epeters2@gmu.edu" 9. 3D"mailto: 10. 3D"mailto:histonet@lists.utsouthwest 11. 3D"mailto:histonet@lists.utsouthweste 12. 3D"mailto:1400521609188.54980@g 13. file://localhost/tmp/3D"htt 14. 3D"mailto:epeters2@gmu.edu" 15. file://localhost/tmp/3D"http 16. file://localhost/tmp/3D"mail 17. file://localhost/tmp/3D"mai 18. 3D"mailto:cecystan76@gmail.com" 19. 3D"mailto:histonet@lists.utsouthwestern.edu" 20. 3D"mailto:Histonet@lists.utsouthwestern.edu" 21. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 22. file://localhost/tmp/3D"mailto 23. 3D"mailto:histonet@lists.utsouthwestern.edu" 24. 3D"mailto:histonet@lists.utsouthwestern.edu" 25. 3D"mailto:tahseen0009@gmail.com" 26. file://localhost/tmp/3D"mail 27. 3D"mailto:Mic 28. 3D"mailto:Sarah.Dysart@stdavids.com" 29. 3D"mailto:Sarah.Dysart@stdavids.com" 30. file://localhost/tmp/3D"mailt 31. file://localhost/tmp/3D"mailto 32. 3D"mailto:B6C82DC18C1D29670A90@AHCMSASEXCH02.my.ahc. 33. 3D"mailto:michael.lafrin 34. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 35. 3D"mailto:Sarah.Dysart@stdavids.com" 36. 3D"mailto:histonet@li 37. 3D"mailto:joyce 38. 3D"http://www.sa=/ 39. 3D"mailto:histonet-bounces@lists.utsout 40. 3D"mailto:histonet-bounces@lists.utsouthwe 41. 3D"mailto:Sarah.Dysart@s 42. file://localhost/tmp/3D"mailto 43. 3D"mailto:Histonet@lists.utsouthwe 44. 3D"http://lists.utsouthwestern.edu/mailman/listin 45. 3D"mailto:Histonet@lists.utsouthwestern.edu" 46. file://localhost/tmp/3D"h 47. 3D"mailto:CThornton@dahlchase.com" 48. 3D"mailto:histonet@lists.utsouthwester 49. 3D"mailto:histonet@lists.utsouthwester 50. ="mailto:84697504013D73DEB4F1@iris.dahlchase.net" 51. 3D"mailto:cthornton@dahlchase.com" 52. ="mailto:cthornton@dahlchase.com" 53. file://localhost/tmp/3D"mailto 54. 3D"mailto:CThornton@dahlchase.com" 55. ="mailto:histonet@lists.utsouthwestern.edu" 56. 3D"mailto:A25377936712AE1BB3CB@EX-MB2.kc 57. 3D"http://labscientific.com/Cryogenic-Suppli 58. 3D"mailto:murphyv@karmanos.org" 59. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 60. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 61. 3D"mailto:CThornton@dahlchase.com" 62. 3D"mailto:histonet@lists.uts 63. 3D"mailto:cthornton@dahlchase.c 64. 3D"mailto:cthornton@dahlchase.com" 65. ="mailto:Histonet@lists.utsouthwestern.edu" 66. 3D"http://list=/ 67. 3D"mailto:daks 68. 3D"mailto:histonet@lists.utsouthwestern.edu" 69. 3D"mailto:407431.JavaMai 70. 3D"mailto:azdudley@hotmail.com" 71. 3D"mailto:Histonet@li 72. 3D"mailto:histonet@lis 73. ="mailto:279A33E8D42CCF0D13D0@phx.gbl" 74. 3D"mailto:amurv 75. 3D"mailto:CThornton@dahlchase.com" 76. 3D"mailto:histonet@lists.utsouthwestern.edu" 77. 3D"mailto:30559B6B81DF07DA897 78. 3D"mailto:histonet-bounces@lists.u 79. 3D"mailto:histonet-bounces@lists 80. 3D"mailto:histonet@ 81. 3D"mailto:cthornton@dahlchase.com" 82. 3D"mailto:Histonet@lists.utsouthwestern.e 83. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/hi 84. file://localhost/tmp/3D"mai 85. 3D"mailto:histonet@lists.utsouthwestern.ed 86. file://localhost/tmp/3D"mai 87. 3D"mailto:SEsparza@seton.org" 88. 3D"mailto:dakshnapriya@neo.ta 89. file://localhost/tmp/3D"mai 90. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 91. file://localhost/tmp/3D 92. 3D"mailto:histonet@lists.utsouthwestern.edu" 93. 3D"mailto:Histonet@lists.utsouthwes 94. 3D"http://lists.utsouthwestern.edu/mailman/listinf 95. 3D"mailto:dakshnapriya@neo.tamu.edu" 96. 3D"mailto:king.laurie@marshfieldclinic.org" 97. 3D"mailto:788819.JavaMail.root@neo. 98. file://localhost/tmp/3D"mailt 99. file://localhost/tmp/3D"mailt 100. 3D"mailto:h 101. 3D"mailto:histonet@lists.utsouthwestern.edu" 102. 3D"mailto:Histonet@lists.utsouthwestern.edu" 103. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 104. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From Joyce.Weems <@t> emoryhealthcare.org Wed May 21 07:25:49 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed May 21 08:06:08 2014 Subject: [Histonet] HTL In-Reply-To: <11682425.1656319.1400669650887.JavaMail.root@vznit170170> References: <11682425.1656319.1400669650887.JavaMail.root@vznit170170> Message-ID: Also the booklets from NSH .. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of madary@verizon.net Sent: Wednesday, May 21, 2014 6:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL &nb=;WHen I took mine i did both parts. for the written carson cover to cover=d the thick bancroft and stevens(sp?) 2 times cover to cover. Nick(Rocky) Madar= HT/HTL(ASCP)QIHC Joni Madary, PhD(in life) <=v style="border-top:1px solid #bcbcbc;margin:5px 0px;"> On 05/20/14, hist=et-request@lists.utsouthwestern.edu wrote: Send Histonet =iling list submissions to [1]histonet@lists.utsout=estern.edu To subscribe or unsubscribe via the World Wide =b, visit [2]http://lists.utsouthwe=ern.edu/mailman/listinfo/histonet or, via email, send a message =th subject or body 'help' to [3]histonet-r=uest@lists.utsouthwestern.edu You can reach the person man=ing the list at [4]histonet-owner@lists.utso=hwestern.edu When replying, please edit your Subject line = it is more specific than "Re: Contents of Histonet digest...&q=t; Today's Topics: 1. HTL certification (C?y Stan) 2. RE: HTL certification (Esther C Peters) 3. pos=ive control indoleamine 2-3 dioxygenase (Pathology-Histology S= Supervisor) 4. immunohistochemisty coding (Michael LaFriniere) 5. antibody vials (Clare Thornton) 6. RE: antibody vials (Mu=hy, Valerie) 7. HELP- Cryosectioning FAT! (Balasubbramanian, Daks=apriya) 8. new processor (anita) 9. RE: antibody vials (A=e Murvosh) 10. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Da=hnapriya) 11. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Dak=napriya) ----------------------------------------------- ----------------------- Message: 1 Date: Mon, 19 May 2014 1=32:36 -0500 From: Cecy Stan <[5]cecystan76@gmail.com> Subject: [Histonet] HTL certification To: [6]histone=lists.utsouthwestern.edu Message-ID: Subject: RE: [Histonet] HTL ce=ification To: Cecy Stan <[9]cecystan76@gmail.com>, =9"[10]histonet@lists.utsouthwestern.edu" <[11]histonet@lists.utsouthwestern.edu> Me=age-ID: <[12]1400521609188.54980@gmu.edu> Content-=pe: text/plain; charset="iso-8859-1" Hi C.A., I don't have the HT or HTL, but from my college teaching experience in histology and histotechniques, I just wanted to caution you that memoriz=g is not what you should be doing. You need to understand concepts, so th= when you need to troubleshoot problems you will be able to think through=ings, rule some things out, and make sense of the situation. I see this =l the time with my students, they forget things they memorize, but then t=y finally understand things and can figure things out. One of the new tea=ing tools is having students prepare "concept maps," to see the=lationships of topics and terms, and these linkages will help you in the=ng run. For histology examples, see: [13]http://www.biologycorner.com/anatomy/tissues/tissue_conce pt_map_samples.html I don't know of any concept maps for his=technology on the web, but I am going to add this to my course next year! Esther Esther C. Peters, Ph.D. Assistant Profes=r Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: D=id King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066e-mail: [14]epeters2@gmu.edu [15]https://bluprd0511.outlook.com/owa/redir.aspx?C =ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKr rDjhw=k.&URL=http%3a%2f%2fesp.gmu.edu ______________=________________________ From: [16]histonet-bo=ces@lists.utsouthwestern.edu <[17]histonet-b ounces@lists.utsouthwestern.edu> on behalf of Cecy Stan <[18]cec ystan76@gmail.com> Sent: Monday, May 19, 2014 1:32 PM To: =9]histonet@lists.utsouthwestern.edu Subject: [Hist=et] HTL certification Hello everyone, I'm starting = prepare for my HTL certification (I am very nervous and anxious but=so very excited about this decision to go for it). I was just=rious to know how you guys prepared for it, and how long it took fo=ou to prepare before taking the test. Will 6 months preparation be =ough? (I know that may depend on the individual; it's just that I ha=y Masters over 10 years ago and I haven't studied this much since t=n). I have Freida L. Carson's 3rd Edition book -- quite daunti= to memorize -- but the outline ASCP has provided for study seems t=e helpful. Do you have any other book/study aid suggestions? <= />Thank you in advance for your input and advice! C.A. _______=______________________________________ Histonet mailing list [20]Histonet@lists.utsouthwestern.edu [21]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Messa=: 3 Date: Tue, 20 May 2014 12:09:28 +0000 From: "Pathology=istology Sr. Supervisor" <[22]histo@skm.org.pk> Subject:=istonet] positive control indoleamine 2-3 dioxygenase To: "[23]histonet@lists.utsouthwestern.edu" <[24]histonet@lists.utsouthwestern.edu> Cc: "[25]tahseen0009@gmail.com" <[26]tahseen0009@gmail.com>Message-ID: Content-Type: text/plain;=arset="us-ascii" Hi All, We are going to opt=ize indoleamine 2-3 dioxygenase (IDO1) expression in breast cancer tissue= immunohistochemistry on formalin fixed paraffin sections. I shall ? thankful if any one please let me know about the "positive control= Regards Muhammad Tahseen Sr.Supervisor Histopathology SKMCH&RC Lahore Pakistan -----------------------=----- Message: 4 Date: Tue, 20 May 2014 13:03:55 +0000 From: Michael LaFriniere <[27]Michael.LaFriniere@ccplab.com= Subject: [Histonet] immunohistochemisty coding To: "=[28]Sarah.Dysart@stdavids.com'" <[29]Sarah.Dysart @stdavids.com>, "[30]histonet@lists.utsou=western.edu" <[31]histonet@lists.utsout=estern.edu> Message-ID: <4A2A16B9707CE04E9C[32]B6C82DC18C1D29670A90@AHCMSASEXCH02.my.ahc.lo cal> Content-Type: text/plain; charset="us-ascii" Hi Sarah, Joyce is correct, under medicare changes in patho=gy coding, you can not bill for each of the blocks of the same antibody a pplications, only one charge per specific antibody mentioned in the patholo= report is allowable. Medicare immuno billing is per "specimen"=ly for each specific antibody used, example if you used s100 for the sam=pecimen (wide melanoma excision)on 15 different blocks, under medicare y= can only bill one GO461, yes- not fair when the need is appropriate, I must voice my opinio)- this probably will lower some of the over utilization and inappropriate billing practices that many of us have seen = the immunohistochemistry world and the labs that have capitalized on the=actice, the problem with the lower reimbursements and change in medicare=ding hurts many of our labs who have used the utilization of immunohisto=emistry appropriately. However, "under current 2014&=ot; CPT coding on non medicare can you bill for the additional work per b=ck using the 88342 and 88343 cpt code, I am sure moving forward we are go=g to continue to see adjustments to the pathology coding processes! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suit? * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Ce= 410-940-8844 [33]michael.lafriniere@CCPLab.com -----Original Message----- From: [34]histonet-bounces@lists.utsouthwestern.edu [ma ilto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joy? K. Sent: Monday, May 19, 2014 12:21 PM To: '[35]Sarah.Dy=rt@stdavids.com'; [36]histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Billing No - one G0461. <= />If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1 and G0462=. j Joyce Weems Pathology Manager 678-843-7376 Pho= 678-843-7831 Fax [37]joyce.weems@emoryhealthcare.o= [38]www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-ma=, including any attachments is the property of Saint Joseph's Hospital an=s intended for the sole use of the intended recipient(s). It may contai=nformation that is privileged and confidential. Any unauthorized review=se, disclosure, or distribution is prohibited. If you are not the intend? recipient, please delete this message, and reply to the sender regarding=e error in a separate email. -----Original Message----- =om: [39]histonet-bounces@lists.utsouthwestern.edu [[40]mailto:histonet-bounces@lists.utsouthwestern.e=] On Behalf Of [41]Sarah.Dysart@stdavids.com Sent: Mon?y, May 19, 2014 12:00 PM To: [42]histonet@lists.utsout=estern.edu Subject: [Histonet] Billing When it comes = the medicare codes...question... So you have one specimen tha=as 10 blocks. AE1/AE3 is ordered on all 10 blocks. Can you bil=E1/AE3 1st Antibody once (G0461), then AE1/AE3 Additional (G0462) nine t=es? Thanks, Sarah E. Dysart, BA, HT (ASCP), QIHC (A=P) Pathology Supervisor St. David's North Austin Medical Center 1222=orth Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing=st [43]Histonet@lists.utsouthwestern.edu [44]http://lists.utsouthwestern.edu/mailman/list=fo/histonet ________________________________ Th= e-mail message (including any attachments) is for the sole use of t= intended recipient(s) and may contain confidential and privileged i=ormation. If the reader of this message is not the intended recipien= you are hereby notified that any dissemination, distribution or cop=ng of this message (including any attachments) is strictly prohibite= If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the or=inal message (including attachments). ________________________=_____________________ Histonet mailing list [45]H=tonet@lists.utsouthwestern.edu [46]http://lists.utsouthwestern.edu/mailman/listinfo/histonet <= /> ------------------------------ Message: 5 Date: =e, 20 May 2014 10:48:20 -0400 From: Clare Thornton <[47]CThor=on@dahlchase.com> Subject: [Histonet] antibody vials To:=48]histonet@lists.utsouthwestern.edu" =9<[49]histonet@lists.utsouthwestern.edu> Mes=ge-ID: Content-Type: text/plain; c=rset="us-ascii" Does anyone have any good ideas fo=eeping the little vials of concentrated antibodies neat and organized in=e fridge? Right now we keep ours in empty pipette tip boxes (without ti=olders) but they're always falling over and mixed up. We use several di?erent vendors for our concentrated antibodies, so our vials are all diffe rent sizes. Thanks for any ideas! Clare J. Thornton=TL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase=agnostic Services 417 State Street, Suite 540 Bangor, ME 04401 [51]cthornton@dahlchase.com<[52]mailto:cthornton@dahl=ase.com> ----------------------------= Message: 6 Date: Tue, 20 May 2014 14:59:21 +0000 Fr=: "Murphy, Valerie" <[53]murphyv@karmanos.org> =bject: [Histonet] RE: antibody vials To: Clare Thornton <[54]=hornton@dahlchase.com>, "histonet@l=ts.utsouthwestern.edu" <[55]histonet@li=s.utsouthwestern.edu> Message-ID: Content-Type: text/plain; charset="us-ascii= I use cardboard cryovial boxes and they work quite well. =u can remove some of the dividers to make space for the larger vials. [57]http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Fre eze=Racks/Cryovial-Boxes-Cardboard/ Valerie =tliff B.Sc HTL(ASCP) Research Assistant Department= Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: [58]murphyv@karmanos.or= Better treatments. Better outcomes. ________________________________________ From: [59]histonet-bounces@lists.utsouthwestern.edu [[60]histonet-bounces@lists.utsouthwestern.edu] on behalf of Clare Thornto=[61]CThornton@dahlchase.com] Sent: Tuesday, May 20, 2014 1=48 AM To: [62]histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody vials Does anyone have any good =eas for keeping the little vials of concentrated antibodies neat and orga=zed in the fridge? Right now we keep ours in empty pipette tip boxes (wi=out tip holders) but they're always falling over and mixed up. We use se=ral different vendors for our concentrated antibodies, so our vials are a= different sizes. Thanks for any ideas! Clare J. =ornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Da=-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, = 04401 [63]cthornton@dahlchase.com<[64]mailto:cthornto=dahlchase.com> __________________________________=___________ Histonet mailing list [65]Histonet@li=s.utsouthwestern.edu [66]http://li=s.utsouthwestern.edu/mailman/listinfo/histonet ----------- Confidentiality Notice: This email message, including any attachments , is for the sole use of the intended recipient(s) and may contain confiden=al and/or privileged information. If you are not the intended recipient(s= you are hereby notified that any dissemination, unauthorized review, use=isclosure or distribution of this email and any materials contained in a= attachments is prohibited. If you receive this message in error, or are =t the intended recipient(s), please immediately notify the sender by emai= and destroy all copies of the original message, including attachments. ------------------------------ Message: 7Date: Tue, 20 May 2014 10:23:27 -0500 (CDT) From: "Balasubbra=nian, Dakshnapriya" <[67]dakshnapriya@neo.tamu.edu><= />Subject: [Histonet] HELP- Cryosectioning FAT! To: [68]histonet@lists.utsouthwestern.edu Message-ID: <21128R24.17352091.1400599[69]407431.JavaMail.root@neo.tamu.edu> Content-Type: text/plain; charset=utf-8 Hi all, I've been having problems with cryosectioning fatty tissue for a lo ng time now. The section leaves a hole in the middle. I know this is probab= a much discussed topic, but I've tried a lot of strategies with no luck.=e tissue is of mouse origin and has micro-lesions buried inside fat. So =e fat needs to be cut in order to get to the lesion. I started with a tem=rature of -17C and tried upto -25C and also at -50C; didn't work at any o=hese temperatures. I tried rubbing the block, blade and anti-roll plate =th dry ice-again,no luck. I rapid-freeze the tissue with OCT = LN2 and then store the block at -80C. Could I try sectioning the block d=ectly from -80C? (never done that). Any other suggestions on =w to tackle this? Any advice is much appreciated!! =anks, Dakshna ------------------------------<= /> Message: 8 Date: Tue, 20 May 2014 10:26:13 -0500 From:=ita <[70]azdudley@hotmail.com> Subject: [Histonet] new p=cessor To: "[71]Histonet@lists.utsouthwestern.edu= <[72]histonet@lists.utsouthwestern.edu<=>> Message-ID: Content-Type: text/plain; charset="iso-885=1" Thanks everyone for your input on processors, I think= will stay with the VIP, it has been a good machine just getting older n=. everyone have a great day!!!! Anita Dudley Providence Hosp Mobile, Alabama 3f95 ------------------------------<= /> Message: 9 Date: Tue, 20 May 2014 08:29:05 -0700 From:=nne Murvosh" <[74]amurvosh@advancederm.net> Subject: RE: [Histonet] antibody vials To: "Clare Thornton"=[75]CThornton@dahlchase.com>, <[76]histonet@lists.utsouthwestern.edu> Message-ID: <4A?A4E531E8C943A7[77]30559B6B81DF07DA8970@dc.Advancederm=et> Content-Type: text/plain; charset="us-ascii"= We got some plexiglass dividers at the "Container Store&q=t; if you have one near or check online. Can't remember what they w=e called but they had different sized square compartments that fit v=ious venders antibodies. Anne -----Original Message-----<= />From: [78]histonet-bounces@lists.utsouthwestern.?u [[79]mailto:histonet-bounces@lists.utsout=estern.edu] On Behalf Of Clare Thornton Sent: Tuesday, May , 2014 7:48 AM To: [80]histonet@lists.utsouthwestern.e= Subject: [Histonet] antibody vials Does anyone have =y good ideas for keeping the little vials of concentrated antibodies=at and organized in the fridge? Right now we keep ours in empty pi=tte tip boxes (without tip holders) but they're always falling over =d mixed up. We use several different vendors for our concentrated a=ibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohi=ochemistry Technologist Dahl-Chase Diagnostic Services 417 Stat=treet, Suite 540 Bangor, ME 04401 cthornton@dahlchase.=m<[81]mailto:cthornton@dahlchase.com> _=____________________________________________ Histonet mailing list[82]Histonet@lists.utsouthwestern.edu [83]http://lists.utsouthwestern.edu/mailman/listinfo/=stonet ------------------------------ Message: 10 Date: Tue, 20 May 2014 10:59:16 -0500 (CDT) From: =alasubbramanian, Dakshnapriya" Subject: Re: [Histonet] HELP- Cryosectioning FAT! To: "Sandra E. Esparza" <[84]SEsparza@seton.org>, =9[85]histonet@lists.utsouthwestern.edu Message-ID: <601376475.17568918.1400601[86]556617.JavaMail.ro=@neo.tamu.edu> Content-Type: text/plain; charset=utf-8 Thanks Sandra! My next step was to try that. Dakshna ----- Original Message ----- From: "Sandra E. Esparza"=[87]SEsparza@seton.org> To: "Dakshnapriya Balasubbram=ian" <[88]dakshnapriya@neo.tamu.edu> Sent: Tues?y, May 20, 2014 10:34:25 AM Subject: RE: [Histonet] HELP- Cryosectio=ng FAT! You might want to dry Liquid Nitrogen on the face of t= block before you cut it. Sandra Sandra Esparza H=ASCP), QIHC Histotechnologist Mohs Austin Dermatologic Surgery?nter 512-324-7468 x84027 [89]sesparza@seton.org -----Original Message----- From: [90]his=net-bounces@lists.utsouthwestern.edu [[91]mailto :histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Balasubbramani=, Dakshnapriya Sent: Tuesday, May 20, 2014 10:23 AM To: [92]histonet@lists.utsouthwestern.edu Subject: [Histonet] =LP- Cryosectioning FAT! Hi all, I've been having pr=lems with cryosectioning fatty tissue for a long time now. The section le=es a hole in the middle. I know this is probably a much discussed topic, =t I've tried a lot of strategies with no luck. The tissue is of mouse ori=n and has micro-lesions buried inside fat. So the fat needs to be cut in =der to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tri? rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the b=ck at -80C. Could I try sectioning the block directly from -80C? (never d=e that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna <= />_______________________________________________ Histonet mailing =st [93]Histonet@lists.utsouthwestern.edu [94]http://lists.utsouthwestern.edu/mailman/listi=o/histonet CONFIDENTIALITY NOTICE: This email m=sage and any accompanying data or files is confidential and may contain p=vileged information intended only for the named recipient(s). If you are =t the intended recipient(s), you are hereby notified that the disseminati=, distribution, and or copying of this message is strictly prohibited. If=u receive this message in error, or are not the named recipient(s), plea= notify the sender at the email address above, delete this email from you=omputer, and destroy any copies in any form immediately. Receipt by anyo= other than the named recipient(s) is not a waiver of any attorney-client=ork product, or other applicable privilege. -----=----------------------- Message: 11 Date: Tue, 20 May 201A1:03:08 -0500 (CDT) From: "Balasubbramanian, Dakshnapriya"= <[95]dakshnapriya@neo.tamu.edu> Subject: Re: [Histon=] HELP- Cryosectioning FAT! To: Laurie J King <[96]k=g.laurie@marshfieldclinic.org>, histonet=ists.utsouthwestern.edu Message-ID: <262036458.175957e.1400601[97]788819.JavaMail.root@neo.tamu.edu> =ntent-Type: text/plain; charset=utf-8 Hi Laurie, =, the tissue hasn't been fixed before freezing. My prof doesn't prefer th=ethod, but if nothing else works, we might give it a try. An=uggested protocols for fixation? Thanks! Dakshna ----- Original Message ----- From: "Laurie J King" <king.laurie@marshfieldclinic.org> To: "Dak=napriya Balasubbramanian" <[98]dakshnapriya@neo.tamu.edu> Sent: Tuesday, May 20, 2014 10:37:35 AM Subject: RE: [Histo=t] HELP- Cryosectioning FAT! Dakshna, Has this tiss= been fixed before freezing by any chance? laurie -=--Original Message----- From: [99]histonet-bou=es@lists.utsouthwestern.edu [[100]mailto:histonet=ounces@lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Daksh=priya Sent: Tuesday, May 20, 2014 10:23 AM To: [101]histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP- Cryo=ctioning FAT! Hi all, I've been having problems wit=ryosectioning fatty tissue for a long time now. The section leaves a hol=n the middle. I know this is probably a much discussed topic, but I've t=ed a lot of strategies with no luck. The tissue is of mouse origin and ha= micro-lesions buried inside fat. So the fat needs to be cut in order to g= to the lesion. I started with a temperature of -17C and tried upto -25C =d also at -50C; didn't work at any of these temperatures. I tried rubbing=e block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -8 . Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any ad=ce is much appreciated!! Thanks, Dakshna _____=________________________________________ Histonet mailing list =02]Histonet@lists.utsouthwestern.edu [103]http://lists.utsouthwestern.edu/mailman/listinfo/histon= _________________________________________________________ _____________ The contents of this message may contain private, protec=d and/or privileged information. If you received this message in error, =u should destroy the e-mail message and any attachments or copies, and yo=re prohibited from retaining, distributing, disclosing or using any info=ation contained within. Please contact the sender and advise of the erro=ous delivery by return e-mail or telephone. Thank you for your cooperati=. ------------------------------ ______=_______________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu [104]http://lists.utsouthwestern.edu/mailman/listinfo/histone= End of Histonet Digest, Vol 126, Issue 20 ***********=**************************** References 1. file://localhost/tmp/3D"mailto 2. 3D"http://lists.utsouthwest= 3. file://localhost/tmp/3D"mai 4. 3D"mailto:histonet- 5. file://localhost/tmp/3D"mai 6. 3D"mailto:histonet@lists.utsouthwestern.edu" 7. 3D"mailto:ApGujjkoEAgjAzA 8. 3D"mailto:epeters2@gmu.edu" 9. 3D"mailto: 10. 3D"mailto:histonet@lists.utsouthwest 11. 3D"mailto:histonet@lists.utsouthweste 12. 3D"mailto:1400521609188.54980@g 13. file://localhost/tmp/3D"htt 14. 3D"mailto:epeters2@gmu.edu" 15. file://localhost/tmp/3D"http 16. file://localhost/tmp/3D"mail 17. file://localhost/tmp/3D"mai 18. 3D"mailto:cecystan76@gmail.com" 19. 3D"mailto:histonet@lists.utsouthwestern.edu" 20. 3D"mailto:Histonet@lists.utsouthwestern.edu" 21. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 22. file://localhost/tmp/3D"mailto 23. 3D"mailto:histonet@lists.utsouthwestern.edu" 24. 3D"mailto:histonet@lists.utsouthwestern.edu" 25. 3D"mailto:tahseen0009@gmail.com" 26. file://localhost/tmp/3D"mail 27. 3D"mailto:Mic 28. 3D"mailto:Sarah.Dysart@stdavids.com" 29. 3D"mailto:Sarah.Dysart@stdavids.com" 30. file://localhost/tmp/3D"mailt 31. file://localhost/tmp/3D"mailto 32. 3D"mailto:B6C82DC18C1D29670A90@AHCMSASEXCH02.my.ahc. 33. 3D"mailto:michael.lafrin 34. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 35. 3D"mailto:Sarah.Dysart@stdavids.com" 36. 3D"mailto:histonet@li 37. 3D"mailto:joyce 38. 3D"http://www.sa= 39. 3D"mailto:histonet-bounces@lists.utsout 40. 3D"mailto:histonet-bounces@lists.utsouthwe 41. 3D"mailto:Sarah.Dysart@s 42. file://localhost/tmp/3D"mailto 43. 3D"mailto:Histonet@lists.utsouthwe 44. 3D"http://lists.utsouthwestern.edu/mailman/listin 45. 3D"mailto:Histonet@lists.utsouthwestern.edu" 46. file://localhost/tmp/3D"h 47. 3D"mailto:CThornton@dahlchase.com" 48. 3D"mailto:histonet@lists.utsouthwester 49. 3D"mailto:histonet@lists.utsouthwester 50. ="mailto:84697504013D73DEB4F1@iris.dahlchase.net" 51. 3D"mailto:cthornton@dahlchase.com" 52. ="mailto:cthornton@dahlchase.com" 53. file://localhost/tmp/3D"mailto 54. 3D"mailto:CThornton@dahlchase.com" 55. ="mailto:histonet@lists.utsouthwestern.edu" 56. 3D"mailto:A25377936712AE1BB3CB@EX-MB2.kc 57. 3D"http://labscientific.com/Cryogenic-Suppli 58. 3D"mailto:murphyv@karmanos.org" 59. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 60. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 61. 3D"mailto:CThornton@dahlchase.com" 62. 3D"mailto:histonet@lists.uts 63. 3D"mailto:cthornton@dahlchase.c 64. 3D"mailto:cthornton@dahlchase.com" 65. ="mailto:Histonet@lists.utsouthwestern.edu" 66. 3D"http://list= 67. 3D"mailto:daks 68. 3D"mailto:histonet@lists.utsouthwestern.edu" 69. 3D"mailto:407431.JavaMai 70. 3D"mailto:azdudley@hotmail.com" 71. 3D"mailto:Histonet@li 72. 3D"mailto:histonet@lis 73. ="mailto:279A33E8D42CCF0D13D0@phx.gbl" 74. 3D"mailto:amurv 75. 3D"mailto:CThornton@dahlchase.com" 76. 3D"mailto:histonet@lists.utsouthwestern.edu" 77. 3D"mailto:30559B6B81DF07DA897 78. 3D"mailto:histonet-bounces@lists.u 79. 3D"mailto:histonet-bounces@lists 80. 3D"mailto:histonet@ 81. 3D"mailto:cthornton@dahlchase.com" 82. 3D"mailto:Histonet@lists.utsouthwestern.e 83. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/hi 84. file://localhost/tmp/3D"mai 85. 3D"mailto:histonet@lists.utsouthwestern.ed 86. file://localhost/tmp/3D"mai 87. 3D"mailto:SEsparza@seton.org" 88. 3D"mailto:dakshnapriya@neo.ta 89. file://localhost/tmp/3D"mai 90. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 91. file://localhost/tmp/3D 92. 3D"mailto:histonet@lists.utsouthwestern.edu" 93. 3D"mailto:Histonet@lists.utsouthwes 94. 3D"http://lists.utsouthwestern.edu/mailman/listinf 95. 3D"mailto:dakshnapriya@neo.tamu.edu" 96. 3D"mailto:king.laurie@marshfieldclinic.org" 97. 3D"mailto:788819.JavaMail.root@neo. 98. file://localhost/tmp/3D"mailt 99. file://localhost/tmp/3D"mailt 100. 3D"mailto:h 101. 3D"mailto:histonet@lists.utsouthwestern.edu" 102. 3D"mailto:Histonet@lists.utsouthwestern.edu" 103. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 104. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Joyce.Weems <@t> emoryhealthcare.org Wed May 21 08:53:18 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed May 21 08:53:30 2014 Subject: [Histonet] HTL In-Reply-To: <3566D9E34287BE4B95372179009446A01DCA7168@EXCHANGE.fmhnt.fmh.org> References: <11682425.1656319.1400669650887.JavaMail.root@vznit170170> <3566D9E34287BE4B95372179009446A01DCA7168@EXCHANGE.fmhnt.fmh.org> Message-ID: My story - I finished my degree late in my career and just wanted to take the registry to see how it was - then I was going to know how to study. I didn't spend much time studying - just brushing up as I took it soon after I graduated and had been doing thesis type reports, etc. I got to the exam - I had to go 100 mi to Knoxville from Kingsport, TN - and they had scheduled me for the wrong day and had all the nursing students there taking their Boards. They told me I could stay and wait till a computer was available - I did. I had taken those books with me, and reviewed as I sat there - learned all kinds of things I used on the test! I passed and didn't have to take it again. It was a miracle!! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Campbell, Tasha M. [mailto:tmcampbell@fmh.org] Sent: Wednesday, May 21, 2014 9:48 AM To: Weems, Joyce K.; madary@verizon.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HTL Definitely the booklets from NSH. Probably the most important, I think. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, May 21, 2014 8:26 AM To: 'madary@verizon.net'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HTL Also the booklets from NSH .. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of madary@verizon.net Sent: Wednesday, May 21, 2014 6:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL &nb=;WHen I took mine i did both parts. for the written carson cover to cover=d the thick bancroft and stevens(sp?) 2 times cover to cover. Nick(Rocky) Madar= HT/HTL(ASCP)QIHC Joni Madary, PhD(in life) <=v style="border-top:1px solid #bcbcbc;margin:5px 0px;"> On 05/20/14, hist=et-request@lists.utsouthwestern.edu wrote: Send Histonet =iling list submissions to [1]histonet@lists.utsout=estern.edu To subscribe or unsubscribe via the World Wide =b, visit [2]http://lists.utsouthwe=ern.edu/mailman/listinfo/histonet or, via email, send a message =th subject or body 'help' to [3]histonet-r=uest@lists.utsouthwestern.edu You can reach the person man=ing the list at [4]histonet-owner@lists.utso=hwestern.edu When replying, please edit your Subject line = it is more specific than "Re: Contents of Histonet digest...&q=t; Today's Topics: 1. HTL certification (C y Stan) 2. RE: HTL certification (Esther C Peters) 3. pos=ive control indoleamine 2-3 dioxygenase (Pathology-Histology S= Supervisor) 4. immunohistochemisty coding (Michael LaFriniere) 5. antibody vials (Clare Thornton) 6. RE: antibody vials (Mu=hy, Valerie) 7. HELP- Cryosectioning FAT! (Balasubbramanian, Daks=apriya) 8. new processor (anita) 9. RE: antibody vials (A=e Murvosh) 10. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Da=hnapriya) 11. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Dak=napriya) ----------------------------------------------- ----------------------- Message: 1 Date: Mon, 19 May 2014 1=32:36 -0500 From: Cecy Stan <[5]cecystan76@gmail.com> Subject: [Histonet] HTL certification To: [6]histone=lists.utsouthwestern.edu Message-ID: Subject: RE: [Histonet] HTL ce=ification To: Cecy Stan <[9]cecystan76@gmail.com>, =9"[10]histonet@lists.utsouthwestern.edu" <[11]histonet@lists.utsouthwestern.edu> Me=age-ID: <[12]1400521609188.54980@gmu.edu> Content-=pe: text/plain; charset="iso-8859-1" Hi C.A., I don't have the HT or HTL, but from my college teaching experience in histology and histotechniques, I just wanted to caution you that memoriz=g is not what you should be doing. You need to understand concepts, so th= when you need to troubleshoot problems you will be able to think through=ings, rule some things out, and make sense of the situation. I see this =l the time with my students, they forget things they memorize, but then t=y finally understand things and can figure things out. One of the new tea=ing tools is having students prepare "concept maps," to see the=lationships of topics and terms, and these linkages will help you in the=ng run. For histology examples, see: [13]http://www.biologycorner.com/anatomy/tissues/tissue_conce pt_map_samples.html I don't know of any concept maps for his=technology on the web, but I am going to add this to my course next year! Esther Esther C. Peters, Ph.D. Assistant Profes=r Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: D=id King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066e-mail: [14]epeters2@gmu.edu [15]https://bluprd0511.outlook.com/owa/redir.aspx?C =ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKr rDjhw=k.&URL=http%3a%2f%2fesp.gmu.edu ______________=________________________ From: [16]histonet-bo=ces@lists.utsouthwestern.edu <[17]histonet-b ounces@lists.utsouthwestern.edu> on behalf of Cecy Stan <[18]cec ystan76@gmail.com> Sent: Monday, May 19, 2014 1:32 PM To: =9]histonet@lists.utsouthwestern.edu Subject: [Hist=et] HTL certification Hello everyone, I'm starting = prepare for my HTL certification (I am very nervous and anxious but=so very excited about this decision to go for it). I was just=rious to know how you guys prepared for it, and how long it took fo=ou to prepare before taking the test. Will 6 months preparation be =ough? (I know that may depend on the individual; it's just that I ha=y Masters over 10 years ago and I haven't studied this much since t=n). I have Freida L. Carson's 3rd Edition book -- quite daunti= to memorize -- but the outline ASCP has provided for study seems t=e helpful. Do you have any other book/study aid suggestions? <= />Thank you in advance for your input and advice! C.A. _______=______________________________________ Histonet mailing list [20]Histonet@lists.utsouthwestern.edu [21]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Messa=: 3 Date: Tue, 20 May 2014 12:09:28 +0000 From: "Pathology=istology Sr. Supervisor" <[22]histo@skm.org.pk> Subject:=istonet] positive control indoleamine 2-3 dioxygenase To: "[23]histonet@lists.utsouthwestern.edu" <[24]histonet@lists.utsouthwestern.edu> Cc: "[25]tahseen0009@gmail.com" <[26]tahseen0009@gmail.com>Message-ID: Content-Type: text/plain;=arset="us-ascii" Hi All, We are going to opt=ize indoleamine 2-3 dioxygenase (IDO1) expression in breast cancer tissue= immunohistochemistry on formalin fixed paraffin sections. I shall thankful if any one please let me know about the "positive control= Regards Muhammad Tahseen Sr.Supervisor Histopathology SKMCH&RC Lahore Pakistan -----------------------=----- Message: 4 Date: Tue, 20 May 2014 13:03:55 +0000 From: Michael LaFriniere <[27]Michael.LaFriniere@ccplab.com= Subject: [Histonet] immunohistochemisty coding To: "=[28]Sarah.Dysart@stdavids.com'" <[29]Sarah.Dysart @stdavids.com>, "[30]histonet@lists.utsou=western.edu" <[31]histonet@lists.utsout=estern.edu> Message-ID: <4A2A16B9707CE04E9C[32]B6C82DC18C1D29670A90@AHCMSASEXCH02.my.ahc.lo cal> Content-Type: text/plain; charset="us-ascii" Hi Sarah, Joyce is correct, under medicare changes in patho=gy coding, you can not bill for each of the blocks of the same antibody a pplications, only one charge per specific antibody mentioned in the patholo= report is allowable. Medicare immuno billing is per "specimen"=ly for each specific antibody used, example if you used s100 for the sam=pecimen (wide melanoma excision)on 15 different blocks, under medicare y= can only bill one GO461, yes- not fair when the need is appropriate, I must voice my opinio)- this probably will lower some of the over utilization and inappropriate billing practices that many of us have seen = the immunohistochemistry world and the labs that have capitalized on the=actice, the problem with the lower reimbursements and change in medicare=ding hurts many of our labs who have used the utilization of immunohisto=emistry appropriately. However, "under current 2014&=ot; CPT coding on non medicare can you bill for the additional work per b=ck using the 88342 and 88343 cpt code, I am sure moving forward we are go=g to continue to see adjustments to the pathology coding processes! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suit * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Ce= 410-940-8844 [33]michael.lafriniere@CCPLab.com -----Original Message----- From: [34]histonet-bounces@lists.utsouthwestern.edu [ma ilto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joy K. Sent: Monday, May 19, 2014 12:21 PM To: '[35]Sarah.Dy=rt@stdavids.com'; [36]histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Billing No - one G0461. <= />If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1 and G0462=. j Joyce Weems Pathology Manager 678-843-7376 Pho= 678-843-7831 Fax [37]joyce.weems@emoryhealthcare.o= [38]www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-ma=, including any attachments is the property of Saint Joseph's Hospital an=s intended for the sole use of the intended recipient(s). It may contai=nformation that is privileged and confidential. Any unauthorized review=se, disclosure, or distribution is prohibited. If you are not the intend recipient, please delete this message, and reply to the sender regarding=e error in a separate email. -----Original Message----- =om: [39]histonet-bounces@lists.utsouthwestern.edu [[40]mailto:histonet-bounces@lists.utsouthwestern.e=] On Behalf Of [41]Sarah.Dysart@stdavids.com Sent: Mon y, May 19, 2014 12:00 PM To: [42]histonet@lists.utsout=estern.edu Subject: [Histonet] Billing When it comes = the medicare codes...question... So you have one specimen tha=as 10 blocks. AE1/AE3 is ordered on all 10 blocks. Can you bil=E1/AE3 1st Antibody once (G0461), then AE1/AE3 Additional (G0462) nine t=es? Thanks, Sarah E. Dysart, BA, HT (ASCP), QIHC (A=P) Pathology Supervisor St. David's North Austin Medical Center 1222=orth Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing=st [43]Histonet@lists.utsouthwestern.edu [44]http://lists.utsouthwestern.edu/mailman/list=fo/histonet ________________________________ Th= e-mail message (including any attachments) is for the sole use of t= intended recipient(s) and may contain confidential and privileged i=ormation. If the reader of this message is not the intended recipien= you are hereby notified that any dissemination, distribution or cop=ng of this message (including any attachments) is strictly prohibite= If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the or=inal message (including attachments). ________________________=_____________________ Histonet mailing list [45]H=tonet@lists.utsouthwestern.edu [46]http://lists.utsouthwestern.edu/mailman/listinfo/histonet <= /> ------------------------------ Message: 5 Date: =e, 20 May 2014 10:48:20 -0400 From: Clare Thornton <[47]CThor=on@dahlchase.com> Subject: [Histonet] antibody vials To:=48]histonet@lists.utsouthwestern.edu" =9<[49]histonet@lists.utsouthwestern.edu> Mes=ge-ID: Content-Type: text/plain; c=rset="us-ascii" Does anyone have any good ideas fo=eeping the little vials of concentrated antibodies neat and organized in=e fridge? Right now we keep ours in empty pipette tip boxes (without ti=olders) but they're always falling over and mixed up. We use several di erent vendors for our concentrated antibodies, so our vials are all diffe rent sizes. Thanks for any ideas! Clare J. Thornton=TL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase=agnostic Services 417 State Street, Suite 540 Bangor, ME 04401 [51]cthornton@dahlchase.com<[52]mailto:cthornton@dahl=ase.com> ----------------------------= Message: 6 Date: Tue, 20 May 2014 14:59:21 +0000 Fr=: "Murphy, Valerie" <[53]murphyv@karmanos.org> =bject: [Histonet] RE: antibody vials To: Clare Thornton <[54]=hornton@dahlchase.com>, "histonet@l=ts.utsouthwestern.edu" <[55]histonet@li=s.utsouthwestern.edu> Message-ID: Content-Type: text/plain; charset="us-ascii= I use cardboard cryovial boxes and they work quite well. =u can remove some of the dividers to make space for the larger vials. [57]http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Fre eze=Racks/Cryovial-Boxes-Cardboard/ Valerie =tliff B.Sc HTL(ASCP) Research Assistant Department= Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: [58]murphyv@karmanos.or= Better treatments. Better outcomes. ________________________________________ From: [59]histonet-bounces@lists.utsouthwestern.edu [[60]histonet-bounces@lists.utsouthwestern.edu] on behalf of Clare Thornto=[61]CThornton@dahlchase.com] Sent: Tuesday, May 20, 2014 1=48 AM To: [62]histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody vials Does anyone have any good =eas for keeping the little vials of concentrated antibodies neat and orga=zed in the fridge? Right now we keep ours in empty pipette tip boxes (wi=out tip holders) but they're always falling over and mixed up. We use se=ral different vendors for our concentrated antibodies, so our vials are a= different sizes. Thanks for any ideas! Clare J. =ornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Da=-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, = 04401 [63]cthornton@dahlchase.com<[64]mailto:cthornto=dahlchase.com> __________________________________=___________ Histonet mailing list [65]Histonet@li=s.utsouthwestern.edu [66]http://li=s.utsouthwestern.edu/mailman/listinfo/histonet ----------- Confidentiality Notice: This email message, including any attachments , is for the sole use of the intended recipient(s) and may contain confiden=al and/or privileged information. If you are not the intended recipient(s= you are hereby notified that any dissemination, unauthorized review, use=isclosure or distribution of this email and any materials contained in a= attachments is prohibited. If you receive this message in error, or are =t the intended recipient(s), please immediately notify the sender by emai= and destroy all copies of the original message, including attachments. ------------------------------ Message: 7Date: Tue, 20 May 2014 10:23:27 -0500 (CDT) From: "Balasubbra=nian, Dakshnapriya" <[67]dakshnapriya@neo.tamu.edu><= />Subject: [Histonet] HELP- Cryosectioning FAT! To: [68]histonet@lists.utsouthwestern.edu Message-ID: <21128R24.17352091.1400599[69]407431.JavaMail.root@neo.tamu.edu> Content-Type: text/plain; charset=utf-8 Hi all, I've been having problems with cryosectioning fatty tissue for a lo ng time now. The section leaves a hole in the middle. I know this is probab= a much discussed topic, but I've tried a lot of strategies with no luck.=e tissue is of mouse origin and has micro-lesions buried inside fat. So =e fat needs to be cut in order to get to the lesion. I started with a tem=rature of -17C and tried upto -25C and also at -50C; didn't work at any o=hese temperatures. I tried rubbing the block, blade and anti-roll plate =th dry ice-again,no luck. I rapid-freeze the tissue with OCT = LN2 and then store the block at -80C. Could I try sectioning the block d=ectly from -80C? (never done that). Any other suggestions on =w to tackle this? Any advice is much appreciated!! =anks, Dakshna ------------------------------<= /> Message: 8 Date: Tue, 20 May 2014 10:26:13 -0500 From:=ita <[70]azdudley@hotmail.com> Subject: [Histonet] new p=cessor To: "[71]Histonet@lists.utsouthwestern.edu= <[72]histonet@lists.utsouthwestern.edu<=>> Message-ID: Content-Type: text/plain; charset="iso-885=1" Thanks everyone for your input on processors, I think= will stay with the VIP, it has been a good machine just getting older n=. everyone have a great day!!!! Anita Dudley Providence Hosp Mobile, Alabama 3f95 ------------------------------<= /> Message: 9 Date: Tue, 20 May 2014 08:29:05 -0700 From:=nne Murvosh" <[74]amurvosh@advancederm.net> Subject: RE: [Histonet] antibody vials To: "Clare Thornton"=[75]CThornton@dahlchase.com>, <[76]histonet@lists.utsouthwestern.edu> Message-ID: <4A A4E531E8C943A7[77]30559B6B81DF07DA8970@dc.Advancederm=et> Content-Type: text/plain; charset="us-ascii"= We got some plexiglass dividers at the "Container Store&q=t; if you have one near or check online. Can't remember what they w=e called but they had different sized square compartments that fit v=ious venders antibodies. Anne -----Original Message-----<= />From: [78]histonet-bounces@lists.utsouthwestern. u [[79]mailto:histonet-bounces@lists.utsout=estern.edu] On Behalf Of Clare Thornton Sent: Tuesday, May , 2014 7:48 AM To: [80]histonet@lists.utsouthwestern.e= Subject: [Histonet] antibody vials Does anyone have =y good ideas for keeping the little vials of concentrated antibodies=at and organized in the fridge? Right now we keep ours in empty pi=tte tip boxes (without tip holders) but they're always falling over =d mixed up. We use several different vendors for our concentrated a=ibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohi=ochemistry Technologist Dahl-Chase Diagnostic Services 417 Stat=treet, Suite 540 Bangor, ME 04401 cthornton@dahlchase.=m<[81]mailto:cthornton@dahlchase.com> _=____________________________________________ Histonet mailing list[82]Histonet@lists.utsouthwestern.edu [83]http://lists.utsouthwestern.edu/mailman/listinfo/=stonet ------------------------------ Message: 10 Date: Tue, 20 May 2014 10:59:16 -0500 (CDT) From: =alasubbramanian, Dakshnapriya" Subject: Re: [Histonet] HELP- Cryosectioning FAT! To: "Sandra E. Esparza" <[84]SEsparza@seton.org>, =9[85]histonet@lists.utsouthwestern.edu Message-ID: <601376475.17568918.1400601[86]556617.JavaMail.ro=@neo.tamu.edu> Content-Type: text/plain; charset=utf-8 Thanks Sandra! My next step was to try that. Dakshna ----- Original Message ----- From: "Sandra E. Esparza"=[87]SEsparza@seton.org> To: "Dakshnapriya Balasubbram=ian" <[88]dakshnapriya@neo.tamu.edu> Sent: Tues y, May 20, 2014 10:34:25 AM Subject: RE: [Histonet] HELP- Cryosectio=ng FAT! You might want to dry Liquid Nitrogen on the face of t= block before you cut it. Sandra Sandra Esparza H=ASCP), QIHC Histotechnologist Mohs Austin Dermatologic Surgery nter 512-324-7468 x84027 [89]sesparza@seton.org -----Original Message----- From: [90]his=net-bounces@lists.utsouthwestern.edu [[91]mailto :histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Balasubbramani=, Dakshnapriya Sent: Tuesday, May 20, 2014 10:23 AM To: [92]histonet@lists.utsouthwestern.edu Subject: [Histonet] =LP- Cryosectioning FAT! Hi all, I've been having pr=lems with cryosectioning fatty tissue for a long time now. The section le=es a hole in the middle. I know this is probably a much discussed topic, =t I've tried a lot of strategies with no luck. The tissue is of mouse ori=n and has micro-lesions buried inside fat. So the fat needs to be cut in =der to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tri rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the b=ck at -80C. Could I try sectioning the block directly from -80C? (never d=e that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna <= />_______________________________________________ Histonet mailing =st [93]Histonet@lists.utsouthwestern.edu [94]http://lists.utsouthwestern.edu/mailman/listi=o/histonet CONFIDENTIALITY NOTICE: This email m=sage and any accompanying data or files is confidential and may contain p=vileged information intended only for the named recipient(s). If you are =t the intended recipient(s), you are hereby notified that the disseminati=, distribution, and or copying of this message is strictly prohibited. If=u receive this message in error, or are not the named recipient(s), plea= notify the sender at the email address above, delete this email from you=omputer, and destroy any copies in any form immediately. Receipt by anyo= other than the named recipient(s) is not a waiver of any attorney-client=ork product, or other applicable privilege. -----=----------------------- Message: 11 Date: Tue, 20 May 201A1:03:08 -0500 (CDT) From: "Balasubbramanian, Dakshnapriya"= <[95]dakshnapriya@neo.tamu.edu> Subject: Re: [Histon=] HELP- Cryosectioning FAT! To: Laurie J King <[96]k=g.laurie@marshfieldclinic.org>, histonet=ists.utsouthwestern.edu Message-ID: <262036458.175957e.1400601[97]788819.JavaMail.root@neo.tamu.edu> =ntent-Type: text/plain; charset=utf-8 Hi Laurie, =, the tissue hasn't been fixed before freezing. My prof doesn't prefer th=ethod, but if nothing else works, we might give it a try. An=uggested protocols for fixation? Thanks! Dakshna ----- Original Message ----- From: "Laurie J King" << lass="parsedEmail" href="mailto:king.laurie@marshfieldclinic.org" ta rget="_blank">king.laurie@marshfieldclinic.org> To: "Dak=napriya Balasubbramanian" <[98]dakshnapriya@neo.tamu.edu> Sent: Tuesday, May 20, 2014 10:37:35 AM Subject: RE: [Histo=t] HELP- Cryosectioning FAT! Dakshna, Has this tiss= been fixed before freezing by any chance? laurie -=--Original Message----- From: [99]histonet-bou=es@lists.utsouthwestern.edu [[100]mailto:histonet=ounces@lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Daksh=priya Sent: Tuesday, May 20, 2014 10:23 AM To: [101]histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP- Cryo=ctioning FAT! Hi all, I've been having problems wit=ryosectioning fatty tissue for a long time now. The section leaves a hol=n the middle. I know this is probably a much discussed topic, but I've t=ed a lot of strategies with no luck. The tissue is of mouse origin and ha= micro-lesions buried inside fat. So the fat needs to be cut in order to g= to the lesion. I started with a temperature of -17C and tried upto -25C =d also at -50C; didn't work at any of these temperatures. I tried rubbing=e block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -8 . Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any ad=ce is much appreciated!! Thanks, Dakshna _____=________________________________________ Histonet mailing list =02]Histonet@lists.utsouthwestern.edu [103]http://lists.utsouthwestern.edu/mailman/listinfo/histon= _________________________________________________________ _____________ The contents of this message may contain private, protec=d and/or privileged information. If you received this message in error, =u should destroy the e-mail message and any attachments or copies, and yo=re prohibited from retaining, distributing, disclosing or using any info=ation contained within. Please contact the sender and advise of the erro=ous delivery by return e-mail or telephone. Thank you for your cooperati=. ------------------------------ ______=_______________________________________ Histonet mailing list < lass="parsedEmail" href="mailto:Histonet@lists.utsouthwestern.edu" t arget="_blank">Histonet@lists.utsouthwestern.edu [104]http://lists.utsouthwestern.edu/mailman/listinfo/histone= End of Histonet Digest, Vol 126, Issue 20 ***********=**************************** References 1. file://localhost/tmp/3D"mailto 2. 3D"http://lists.utsouthwest= 3. file://localhost/tmp/3D"mai 4. 3D"mailto:histonet- 5. file://localhost/tmp/3D"mai 6. 3D"mailto:histonet@lists.utsouthwestern.edu" 7. 3D"mailto:ApGujjkoEAgjAzA 8. 3D"mailto:epeters2@gmu.edu" 9. 3D"mailto: 10. 3D"mailto:histonet@lists.utsouthwest 11. 3D"mailto:histonet@lists.utsouthweste 12. 3D"mailto:1400521609188.54980@g 13. file://localhost/tmp/3D"htt 14. 3D"mailto:epeters2@gmu.edu" 15. file://localhost/tmp/3D"http 16. file://localhost/tmp/3D"mail 17. file://localhost/tmp/3D"mai 18. 3D"mailto:cecystan76@gmail.com" 19. 3D"mailto:histonet@lists.utsouthwestern.edu" 20. 3D"mailto:Histonet@lists.utsouthwestern.edu" 21. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 22. file://localhost/tmp/3D"mailto 23. 3D"mailto:histonet@lists.utsouthwestern.edu" 24. 3D"mailto:histonet@lists.utsouthwestern.edu" 25. 3D"mailto:tahseen0009@gmail.com" 26. file://localhost/tmp/3D"mail 27. 3D"mailto:Mic 28. 3D"mailto:Sarah.Dysart@stdavids.com" 29. 3D"mailto:Sarah.Dysart@stdavids.com" 30. file://localhost/tmp/3D"mailt 31. file://localhost/tmp/3D"mailto 32. 3D"mailto:B6C82DC18C1D29670A90@AHCMSASEXCH02.my.ahc. 33. 3D"mailto:michael.lafrin 34. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 35. 3D"mailto:Sarah.Dysart@stdavids.com" 36. 3D"mailto:histonet@li 37. 3D"mailto:joyce 38. 3D"http://www.sa= 39. 3D"mailto:histonet-bounces@lists.utsout 40. 3D"mailto:histonet-bounces@lists.utsouthwe 41. 3D"mailto:Sarah.Dysart@s 42. file://localhost/tmp/3D"mailto 43. 3D"mailto:Histonet@lists.utsouthwe 44. 3D"http://lists.utsouthwestern.edu/mailman/listin 45. 3D"mailto:Histonet@lists.utsouthwestern.edu" 46. file://localhost/tmp/3D"h 47. 3D"mailto:CThornton@dahlchase.com" 48. 3D"mailto:histonet@lists.utsouthwester 49. 3D"mailto:histonet@lists.utsouthwester 50. ="mailto:84697504013D73DEB4F1@iris.dahlchase.net" 51. 3D"mailto:cthornton@dahlchase.com" 52. ="mailto:cthornton@dahlchase.com" 53. file://localhost/tmp/3D"mailto 54. 3D"mailto:CThornton@dahlchase.com" 55. ="mailto:histonet@lists.utsouthwestern.edu" 56. 3D"mailto:A25377936712AE1BB3CB@EX-MB2.kc 57. 3D"http://labscientific.com/Cryogenic-Suppli 58. 3D"mailto:murphyv@karmanos.org" 59. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 60. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 61. 3D"mailto:CThornton@dahlchase.com" 62. 3D"mailto:histonet@lists.uts 63. 3D"mailto:cthornton@dahlchase.c 64. 3D"mailto:cthornton@dahlchase.com" 65. ="mailto:Histonet@lists.utsouthwestern.edu" 66. 3D"http://list= 67. 3D"mailto:daks 68. 3D"mailto:histonet@lists.utsouthwestern.edu" 69. 3D"mailto:407431.JavaMai 70. 3D"mailto:azdudley@hotmail.com" 71. 3D"mailto:Histonet@li 72. 3D"mailto:histonet@lis 73. ="mailto:279A33E8D42CCF0D13D0@phx.gbl" 74. 3D"mailto:amurv 75. 3D"mailto:CThornton@dahlchase.com" 76. 3D"mailto:histonet@lists.utsouthwestern.edu" 77. 3D"mailto:30559B6B81DF07DA897 78. 3D"mailto:histonet-bounces@lists.u 79. 3D"mailto:histonet-bounces@lists 80. 3D"mailto:histonet@ 81. 3D"mailto:cthornton@dahlchase.com" 82. 3D"mailto:Histonet@lists.utsouthwestern.e 83. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/hi 84. file://localhost/tmp/3D"mai 85. 3D"mailto:histonet@lists.utsouthwestern.ed 86. file://localhost/tmp/3D"mai 87. 3D"mailto:SEsparza@seton.org" 88. 3D"mailto:dakshnapriya@neo.ta 89. file://localhost/tmp/3D"mai 90. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 91. file://localhost/tmp/3D 92. 3D"mailto:histonet@lists.utsouthwestern.edu" 93. 3D"mailto:Histonet@lists.utsouthwes 94. 3D"http://lists.utsouthwestern.edu/mailman/listinf 95. 3D"mailto:dakshnapriya@neo.tamu.edu" 96. 3D"mailto:king.laurie@marshfieldclinic.org" 97. 3D"mailto:788819.JavaMail.root@neo. 98. file://localhost/tmp/3D"mailt 99. file://localhost/tmp/3D"mailt 100. 3D"mailto:h 101. 3D"mailto:histonet@lists.utsouthwestern.edu" 102. 3D"mailto:Histonet@lists.utsouthwestern.edu" 103. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 104. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From eca9 <@t> georgetown.edu Wed May 21 08:53:51 2014 From: eca9 <@t> georgetown.edu (Eva Permaul) Date: Wed May 21 08:54:34 2014 Subject: [Histonet] HA-tag antibody Message-ID: Hello, I am looking for an HA-tag antibody to detect a protein labeled with HA in formalin fixed paraffin embedded tissues. Could anyone suggest a published antibody? The tissue of interest is mouse so I would prefer an antibody made in another species. I have found several antibodies but can't find them published. Thank you, Eva Georgetown University From tmcampbell <@t> fmh.org Wed May 21 08:48:14 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Wed May 21 09:21:17 2014 Subject: [Histonet] HTL In-Reply-To: References: <11682425.1656319.1400669650887.JavaMail.root@vznit170170> Message-ID: <3566D9E34287BE4B95372179009446A01DCA7168@EXCHANGE.fmhnt.fmh.org> Definitely the booklets from NSH. Probably the most important, I think. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, May 21, 2014 8:26 AM To: 'madary@verizon.net'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HTL Also the booklets from NSH .. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of madary@verizon.net Sent: Wednesday, May 21, 2014 6:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL &nb=;WHen I took mine i did both parts. for the written carson cover to cover=d the thick bancroft and stevens(sp?) 2 times cover to cover. Nick(Rocky) Madar= HT/HTL(ASCP)QIHC Joni Madary, PhD(in life) <=v style="border-top:1px solid #bcbcbc;margin:5px 0px;"> On 05/20/14, hist=et-request@lists.utsouthwestern.edu wrote: Send Histonet =iling list submissions to [1]histonet@lists.utsout=estern.edu To subscribe or unsubscribe via the World Wide =b, visit [2]http://lists.utsouthwe=ern.edu/mailman/listinfo/histonet or, via email, send a message =th subject or body 'help' to [3]histonet-r=uest@lists.utsouthwestern.edu You can reach the person man=ing the list at [4]histonet-owner@lists.utso=hwestern.edu When replying, please edit your Subject line = it is more specific than "Re: Contents of Histonet digest...&q=t; Today's Topics: 1. HTL certification (C?y Stan) 2. RE: HTL certification (Esther C Peters) 3. pos=ive control indoleamine 2-3 dioxygenase (Pathology-Histology S= Supervisor) 4. immunohistochemisty coding (Michael LaFriniere) 5. antibody vials (Clare Thornton) 6. RE: antibody vials (Mu=hy, Valerie) 7. HELP- Cryosectioning FAT! (Balasubbramanian, Daks=apriya) 8. new processor (anita) 9. RE: antibody vials (A=e Murvosh) 10. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Da=hnapriya) 11. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Dak=napriya) ----------------------------------------------- ----------------------- Message: 1 Date: Mon, 19 May 2014 1=32:36 -0500 From: Cecy Stan <[5]cecystan76@gmail.com> Subject: [Histonet] HTL certification To: [6]histone=lists.utsouthwestern.edu Message-ID: Subject: RE: [Histonet] HTL ce=ification To: Cecy Stan <[9]cecystan76@gmail.com>, =9"[10]histonet@lists.utsouthwestern.edu" <[11]histonet@lists.utsouthwestern.edu> Me=age-ID: <[12]1400521609188.54980@gmu.edu> Content-=pe: text/plain; charset="iso-8859-1" Hi C.A., I don't have the HT or HTL, but from my college teaching experience in histology and histotechniques, I just wanted to caution you that memoriz=g is not what you should be doing. You need to understand concepts, so th= when you need to troubleshoot problems you will be able to think through=ings, rule some things out, and make sense of the situation. I see this =l the time with my students, they forget things they memorize, but then t=y finally understand things and can figure things out. One of the new tea=ing tools is having students prepare "concept maps," to see the=lationships of topics and terms, and these linkages will help you in the=ng run. For histology examples, see: [13]http://www.biologycorner.com/anatomy/tissues/tissue_conce pt_map_samples.html I don't know of any concept maps for his=technology on the web, but I am going to add this to my course next year! Esther Esther C. Peters, Ph.D. Assistant Profes=r Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: D=id King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066e-mail: [14]epeters2@gmu.edu [15]https://bluprd0511.outlook.com/owa/redir.aspx?C =ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKr rDjhw=k.&URL=http%3a%2f%2fesp.gmu.edu ______________=________________________ From: [16]histonet-bo=ces@lists.utsouthwestern.edu <[17]histonet-b ounces@lists.utsouthwestern.edu> on behalf of Cecy Stan <[18]cec ystan76@gmail.com> Sent: Monday, May 19, 2014 1:32 PM To: =9]histonet@lists.utsouthwestern.edu Subject: [Hist=et] HTL certification Hello everyone, I'm starting = prepare for my HTL certification (I am very nervous and anxious but=so very excited about this decision to go for it). I was just=rious to know how you guys prepared for it, and how long it took fo=ou to prepare before taking the test. Will 6 months preparation be =ough? (I know that may depend on the individual; it's just that I ha=y Masters over 10 years ago and I haven't studied this much since t=n). I have Freida L. Carson's 3rd Edition book -- quite daunti= to memorize -- but the outline ASCP has provided for study seems t=e helpful. Do you have any other book/study aid suggestions? <= />Thank you in advance for your input and advice! C.A. _______=______________________________________ Histonet mailing list [20]Histonet@lists.utsouthwestern.edu [21]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Messa=: 3 Date: Tue, 20 May 2014 12:09:28 +0000 From: "Pathology=istology Sr. Supervisor" <[22]histo@skm.org.pk> Subject:=istonet] positive control indoleamine 2-3 dioxygenase To: "[23]histonet@lists.utsouthwestern.edu" <[24]histonet@lists.utsouthwestern.edu> Cc: "[25]tahseen0009@gmail.com" <[26]tahseen0009@gmail.com>Message-ID: Content-Type: text/plain;=arset="us-ascii" Hi All, We are going to opt=ize indoleamine 2-3 dioxygenase (IDO1) expression in breast cancer tissue= immunohistochemistry on formalin fixed paraffin sections. I shall ? thankful if any one please let me know about the "positive control= Regards Muhammad Tahseen Sr.Supervisor Histopathology SKMCH&RC Lahore Pakistan -----------------------=----- Message: 4 Date: Tue, 20 May 2014 13:03:55 +0000 From: Michael LaFriniere <[27]Michael.LaFriniere@ccplab.com= Subject: [Histonet] immunohistochemisty coding To: "=[28]Sarah.Dysart@stdavids.com'" <[29]Sarah.Dysart @stdavids.com>, "[30]histonet@lists.utsou=western.edu" <[31]histonet@lists.utsout=estern.edu> Message-ID: <4A2A16B9707CE04E9C[32]B6C82DC18C1D29670A90@AHCMSASEXCH02.my.ahc.lo cal> Content-Type: text/plain; charset="us-ascii" Hi Sarah, Joyce is correct, under medicare changes in patho=gy coding, you can not bill for each of the blocks of the same antibody a pplications, only one charge per specific antibody mentioned in the patholo= report is allowable. Medicare immuno billing is per "specimen"=ly for each specific antibody used, example if you used s100 for the sam=pecimen (wide melanoma excision)on 15 different blocks, under medicare y= can only bill one GO461, yes- not fair when the need is appropriate, I must voice my opinio)- this probably will lower some of the over utilization and inappropriate billing practices that many of us have seen = the immunohistochemistry world and the labs that have capitalized on the=actice, the problem with the lower reimbursements and change in medicare=ding hurts many of our labs who have used the utilization of immunohisto=emistry appropriately. However, "under current 2014&=ot; CPT coding on non medicare can you bill for the additional work per b=ck using the 88342 and 88343 cpt code, I am sure moving forward we are go=g to continue to see adjustments to the pathology coding processes! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suit? * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Ce= 410-940-8844 [33]michael.lafriniere@CCPLab.com -----Original Message----- From: [34]histonet-bounces@lists.utsouthwestern.edu [ma ilto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joy? K. Sent: Monday, May 19, 2014 12:21 PM To: '[35]Sarah.Dy=rt@stdavids.com'; [36]histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Billing No - one G0461. <= />If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1 and G0462=. j Joyce Weems Pathology Manager 678-843-7376 Pho= 678-843-7831 Fax [37]joyce.weems@emoryhealthcare.o= [38]www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-ma=, including any attachments is the property of Saint Joseph's Hospital an=s intended for the sole use of the intended recipient(s). It may contai=nformation that is privileged and confidential. Any unauthorized review=se, disclosure, or distribution is prohibited. If you are not the intend? recipient, please delete this message, and reply to the sender regarding=e error in a separate email. -----Original Message----- =om: [39]histonet-bounces@lists.utsouthwestern.edu [[40]mailto:histonet-bounces@lists.utsouthwestern.e=] On Behalf Of [41]Sarah.Dysart@stdavids.com Sent: Mon?y, May 19, 2014 12:00 PM To: [42]histonet@lists.utsout=estern.edu Subject: [Histonet] Billing When it comes = the medicare codes...question... So you have one specimen tha=as 10 blocks. AE1/AE3 is ordered on all 10 blocks. Can you bil=E1/AE3 1st Antibody once (G0461), then AE1/AE3 Additional (G0462) nine t=es? Thanks, Sarah E. Dysart, BA, HT (ASCP), QIHC (A=P) Pathology Supervisor St. David's North Austin Medical Center 1222=orth Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing=st [43]Histonet@lists.utsouthwestern.edu [44]http://lists.utsouthwestern.edu/mailman/list=fo/histonet ________________________________ Th= e-mail message (including any attachments) is for the sole use of t= intended recipient(s) and may contain confidential and privileged i=ormation. If the reader of this message is not the intended recipien= you are hereby notified that any dissemination, distribution or cop=ng of this message (including any attachments) is strictly prohibite= If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the or=inal message (including attachments). ________________________=_____________________ Histonet mailing list [45]H=tonet@lists.utsouthwestern.edu [46]http://lists.utsouthwestern.edu/mailman/listinfo/histonet <= /> ------------------------------ Message: 5 Date: =e, 20 May 2014 10:48:20 -0400 From: Clare Thornton <[47]CThor=on@dahlchase.com> Subject: [Histonet] antibody vials To:=48]histonet@lists.utsouthwestern.edu" =9<[49]histonet@lists.utsouthwestern.edu> Mes=ge-ID: Content-Type: text/plain; c=rset="us-ascii" Does anyone have any good ideas fo=eeping the little vials of concentrated antibodies neat and organized in=e fridge? Right now we keep ours in empty pipette tip boxes (without ti=olders) but they're always falling over and mixed up. We use several di?erent vendors for our concentrated antibodies, so our vials are all diffe rent sizes. Thanks for any ideas! Clare J. Thornton=TL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase=agnostic Services 417 State Street, Suite 540 Bangor, ME 04401 [51]cthornton@dahlchase.com<[52]mailto:cthornton@dahl=ase.com> ----------------------------= Message: 6 Date: Tue, 20 May 2014 14:59:21 +0000 Fr=: "Murphy, Valerie" <[53]murphyv@karmanos.org> =bject: [Histonet] RE: antibody vials To: Clare Thornton <[54]=hornton@dahlchase.com>, "histonet@l=ts.utsouthwestern.edu" <[55]histonet@li=s.utsouthwestern.edu> Message-ID: Content-Type: text/plain; charset="us-ascii= I use cardboard cryovial boxes and they work quite well. =u can remove some of the dividers to make space for the larger vials. [57]http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Fre eze=Racks/Cryovial-Boxes-Cardboard/ Valerie =tliff B.Sc HTL(ASCP) Research Assistant Department= Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: [58]murphyv@karmanos.or= Better treatments. Better outcomes. ________________________________________ From: [59]histonet-bounces@lists.utsouthwestern.edu [[60]histonet-bounces@lists.utsouthwestern.edu] on behalf of Clare Thornto=[61]CThornton@dahlchase.com] Sent: Tuesday, May 20, 2014 1=48 AM To: [62]histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody vials Does anyone have any good =eas for keeping the little vials of concentrated antibodies neat and orga=zed in the fridge? Right now we keep ours in empty pipette tip boxes (wi=out tip holders) but they're always falling over and mixed up. We use se=ral different vendors for our concentrated antibodies, so our vials are a= different sizes. Thanks for any ideas! Clare J. =ornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Da=-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, = 04401 [63]cthornton@dahlchase.com<[64]mailto:cthornto=dahlchase.com> __________________________________=___________ Histonet mailing list [65]Histonet@li=s.utsouthwestern.edu [66]http://li=s.utsouthwestern.edu/mailman/listinfo/histonet ----------- Confidentiality Notice: This email message, including any attachments , is for the sole use of the intended recipient(s) and may contain confiden=al and/or privileged information. If you are not the intended recipient(s= you are hereby notified that any dissemination, unauthorized review, use=isclosure or distribution of this email and any materials contained in a= attachments is prohibited. If you receive this message in error, or are =t the intended recipient(s), please immediately notify the sender by emai= and destroy all copies of the original message, including attachments. ------------------------------ Message: 7Date: Tue, 20 May 2014 10:23:27 -0500 (CDT) From: "Balasubbra=nian, Dakshnapriya" <[67]dakshnapriya@neo.tamu.edu><= />Subject: [Histonet] HELP- Cryosectioning FAT! To: [68]histonet@lists.utsouthwestern.edu Message-ID: <21128R24.17352091.1400599[69]407431.JavaMail.root@neo.tamu.edu> Content-Type: text/plain; charset=utf-8 Hi all, I've been having problems with cryosectioning fatty tissue for a lo ng time now. The section leaves a hole in the middle. I know this is probab= a much discussed topic, but I've tried a lot of strategies with no luck.=e tissue is of mouse origin and has micro-lesions buried inside fat. So =e fat needs to be cut in order to get to the lesion. I started with a tem=rature of -17C and tried upto -25C and also at -50C; didn't work at any o=hese temperatures. I tried rubbing the block, blade and anti-roll plate =th dry ice-again,no luck. I rapid-freeze the tissue with OCT = LN2 and then store the block at -80C. Could I try sectioning the block d=ectly from -80C? (never done that). Any other suggestions on =w to tackle this? Any advice is much appreciated!! =anks, Dakshna ------------------------------<= /> Message: 8 Date: Tue, 20 May 2014 10:26:13 -0500 From:=ita <[70]azdudley@hotmail.com> Subject: [Histonet] new p=cessor To: "[71]Histonet@lists.utsouthwestern.edu= <[72]histonet@lists.utsouthwestern.edu<=>> Message-ID: Content-Type: text/plain; charset="iso-885=1" Thanks everyone for your input on processors, I think= will stay with the VIP, it has been a good machine just getting older n=. everyone have a great day!!!! Anita Dudley Providence Hosp Mobile, Alabama 3f95 ------------------------------<= /> Message: 9 Date: Tue, 20 May 2014 08:29:05 -0700 From:=nne Murvosh" <[74]amurvosh@advancederm.net> Subject: RE: [Histonet] antibody vials To: "Clare Thornton"=[75]CThornton@dahlchase.com>, <[76]histonet@lists.utsouthwestern.edu> Message-ID: <4A?A4E531E8C943A7[77]30559B6B81DF07DA8970@dc.Advancederm=et> Content-Type: text/plain; charset="us-ascii"= We got some plexiglass dividers at the "Container Store&q=t; if you have one near or check online. Can't remember what they w=e called but they had different sized square compartments that fit v=ious venders antibodies. Anne -----Original Message-----<= />From: [78]histonet-bounces@lists.utsouthwestern.?u [[79]mailto:histonet-bounces@lists.utsout=estern.edu] On Behalf Of Clare Thornton Sent: Tuesday, May , 2014 7:48 AM To: [80]histonet@lists.utsouthwestern.e= Subject: [Histonet] antibody vials Does anyone have =y good ideas for keeping the little vials of concentrated antibodies=at and organized in the fridge? Right now we keep ours in empty pi=tte tip boxes (without tip holders) but they're always falling over =d mixed up. We use several different vendors for our concentrated a=ibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohi=ochemistry Technologist Dahl-Chase Diagnostic Services 417 Stat=treet, Suite 540 Bangor, ME 04401 cthornton@dahlchase.=m<[81]mailto:cthornton@dahlchase.com> _=____________________________________________ Histonet mailing list[82]Histonet@lists.utsouthwestern.edu [83]http://lists.utsouthwestern.edu/mailman/listinfo/=stonet ------------------------------ Message: 10 Date: Tue, 20 May 2014 10:59:16 -0500 (CDT) From: =alasubbramanian, Dakshnapriya" Subject: Re: [Histonet] HELP- Cryosectioning FAT! To: "Sandra E. Esparza" <[84]SEsparza@seton.org>, =9[85]histonet@lists.utsouthwestern.edu Message-ID: <601376475.17568918.1400601[86]556617.JavaMail.ro=@neo.tamu.edu> Content-Type: text/plain; charset=utf-8 Thanks Sandra! My next step was to try that. Dakshna ----- Original Message ----- From: "Sandra E. Esparza"=[87]SEsparza@seton.org> To: "Dakshnapriya Balasubbram=ian" <[88]dakshnapriya@neo.tamu.edu> Sent: Tues?y, May 20, 2014 10:34:25 AM Subject: RE: [Histonet] HELP- Cryosectio=ng FAT! You might want to dry Liquid Nitrogen on the face of t= block before you cut it. Sandra Sandra Esparza H=ASCP), QIHC Histotechnologist Mohs Austin Dermatologic Surgery?nter 512-324-7468 x84027 [89]sesparza@seton.org -----Original Message----- From: [90]his=net-bounces@lists.utsouthwestern.edu [[91]mailto :histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Balasubbramani=, Dakshnapriya Sent: Tuesday, May 20, 2014 10:23 AM To: [92]histonet@lists.utsouthwestern.edu Subject: [Histonet] =LP- Cryosectioning FAT! Hi all, I've been having pr=lems with cryosectioning fatty tissue for a long time now. The section le=es a hole in the middle. I know this is probably a much discussed topic, =t I've tried a lot of strategies with no luck. The tissue is of mouse ori=n and has micro-lesions buried inside fat. So the fat needs to be cut in =der to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tri? rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the b=ck at -80C. Could I try sectioning the block directly from -80C? (never d=e that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna <= />_______________________________________________ Histonet mailing =st [93]Histonet@lists.utsouthwestern.edu [94]http://lists.utsouthwestern.edu/mailman/listi=o/histonet CONFIDENTIALITY NOTICE: This email m=sage and any accompanying data or files is confidential and may contain p=vileged information intended only for the named recipient(s). If you are =t the intended recipient(s), you are hereby notified that the disseminati=, distribution, and or copying of this message is strictly prohibited. If=u receive this message in error, or are not the named recipient(s), plea= notify the sender at the email address above, delete this email from you=omputer, and destroy any copies in any form immediately. Receipt by anyo= other than the named recipient(s) is not a waiver of any attorney-client=ork product, or other applicable privilege. -----=----------------------- Message: 11 Date: Tue, 20 May 201A1:03:08 -0500 (CDT) From: "Balasubbramanian, Dakshnapriya"= <[95]dakshnapriya@neo.tamu.edu> Subject: Re: [Histon=] HELP- Cryosectioning FAT! To: Laurie J King <[96]k=g.laurie@marshfieldclinic.org>, histonet=ists.utsouthwestern.edu Message-ID: <262036458.175957e.1400601[97]788819.JavaMail.root@neo.tamu.edu> =ntent-Type: text/plain; charset=utf-8 Hi Laurie, =, the tissue hasn't been fixed before freezing. My prof doesn't prefer th=ethod, but if nothing else works, we might give it a try. An=uggested protocols for fixation? Thanks! Dakshna ----- Original Message ----- From: "Laurie J King" <king.laurie@marshfieldclinic.org> To: "Dak=napriya Balasubbramanian" <[98]dakshnapriya@neo.tamu.edu> Sent: Tuesday, May 20, 2014 10:37:35 AM Subject: RE: [Histo=t] HELP- Cryosectioning FAT! Dakshna, Has this tiss= been fixed before freezing by any chance? laurie -=--Original Message----- From: [99]histonet-bou=es@lists.utsouthwestern.edu [[100]mailto:histonet=ounces@lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Daksh=priya Sent: Tuesday, May 20, 2014 10:23 AM To: [101]histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP- Cryo=ctioning FAT! Hi all, I've been having problems wit=ryosectioning fatty tissue for a long time now. The section leaves a hol=n the middle. I know this is probably a much discussed topic, but I've t=ed a lot of strategies with no luck. The tissue is of mouse origin and ha= micro-lesions buried inside fat. So the fat needs to be cut in order to g= to the lesion. I started with a temperature of -17C and tried upto -25C =d also at -50C; didn't work at any of these temperatures. I tried rubbing=e block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -8 . Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any ad=ce is much appreciated!! Thanks, Dakshna _____=________________________________________ Histonet mailing list =02]Histonet@lists.utsouthwestern.edu [103]http://lists.utsouthwestern.edu/mailman/listinfo/histon= _________________________________________________________ _____________ The contents of this message may contain private, protec=d and/or privileged information. If you received this message in error, =u should destroy the e-mail message and any attachments or copies, and yo=re prohibited from retaining, distributing, disclosing or using any info=ation contained within. Please contact the sender and advise of the erro=ous delivery by return e-mail or telephone. Thank you for your cooperati=. ------------------------------ ______=_______________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu [104]http://lists.utsouthwestern.edu/mailman/listinfo/histone= End of Histonet Digest, Vol 126, Issue 20 ***********=**************************** References 1. file://localhost/tmp/3D"mailto 2. 3D"http://lists.utsouthwest= 3. file://localhost/tmp/3D"mai 4. 3D"mailto:histonet- 5. file://localhost/tmp/3D"mai 6. 3D"mailto:histonet@lists.utsouthwestern.edu" 7. 3D"mailto:ApGujjkoEAgjAzA 8. 3D"mailto:epeters2@gmu.edu" 9. 3D"mailto: 10. 3D"mailto:histonet@lists.utsouthwest 11. 3D"mailto:histonet@lists.utsouthweste 12. 3D"mailto:1400521609188.54980@g 13. file://localhost/tmp/3D"htt 14. 3D"mailto:epeters2@gmu.edu" 15. file://localhost/tmp/3D"http 16. file://localhost/tmp/3D"mail 17. file://localhost/tmp/3D"mai 18. 3D"mailto:cecystan76@gmail.com" 19. 3D"mailto:histonet@lists.utsouthwestern.edu" 20. 3D"mailto:Histonet@lists.utsouthwestern.edu" 21. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" 22. file://localhost/tmp/3D"mailto 23. 3D"mailto:histonet@lists.utsouthwestern.edu" 24. 3D"mailto:histonet@lists.utsouthwestern.edu" 25. 3D"mailto:tahseen0009@gmail.com" 26. file://localhost/tmp/3D"mail 27. 3D"mailto:Mic 28. 3D"mailto:Sarah.Dysart@stdavids.com" 29. 3D"mailto:Sarah.Dysart@stdavids.com" 30. file://localhost/tmp/3D"mailt 31. file://localhost/tmp/3D"mailto 32. 3D"mailto:B6C82DC18C1D29670A90@AHCMSASEXCH02.my.ahc. 33. 3D"mailto:michael.lafrin 34. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 35. 3D"mailto:Sarah.Dysart@stdavids.com" 36. 3D"mailto:histonet@li 37. 3D"mailto:joyce 38. 3D"http://www.sa= 39. 3D"mailto:histonet-bounces@lists.utsout 40. 3D"mailto:histonet-bounces@lists.utsouthwe 41. 3D"mailto:Sarah.Dysart@s 42. file://localhost/tmp/3D"mailto 43. 3D"mailto:Histonet@lists.utsouthwe 44. 3D"http://lists.utsouthwestern.edu/mailman/listin 45. 3D"mailto:Histonet@lists.utsouthwestern.edu" 46. file://localhost/tmp/3D"h 47. 3D"mailto:CThornton@dahlchase.com" 48. 3D"mailto:histonet@lists.utsouthwester 49. 3D"mailto:histonet@lists.utsouthwester 50. ="mailto:84697504013D73DEB4F1@iris.dahlchase.net" 51. 3D"mailto:cthornton@dahlchase.com" 52. ="mailto:cthornton@dahlchase.com" 53. file://localhost/tmp/3D"mailto 54. 3D"mailto:CThornton@dahlchase.com" 55. ="mailto:histonet@lists.utsouthwestern.edu" 56. 3D"mailto:A25377936712AE1BB3CB@EX-MB2.kc 57. 3D"http://labscientific.com/Cryogenic-Suppli 58. 3D"mailto:murphyv@karmanos.org" 59. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 60. 3D"mailto:histonet-bounces@lists.utsouthwestern.edu" 61. 3D"mailto:CThornton@dahlchase.com" 62. 3D"mailto:histonet@lists.uts 63. 3D"mailto:cthornton@dahlchase.c 64. 3D"mailto:cthornton@dahlchase.com" 65. ="mailto:Histonet@lists.utsouthwestern.edu" 66. 3D"http://list= 67. 3D"mailto:daks 68. 3D"mailto:histonet@lists.utsouthwestern.edu" 69. 3D"mailto:407431.JavaMai 70. 3D"mailto:azdudley@hotmail.com" 71. 3D"mailto:Histonet@li 72. 3D"mailto:histonet@lis 73. ="mailto:279A33E8D42CCF0D13D0@phx.gbl" 74. 3D"mailto:amurv 75. 3D"mailto:CThornton@dahlchase.com" 76. 3D"mailto:histonet@lists.utsouthwestern.edu" 77. 3D"mailto:30559B6B81DF07DA897 78. 3D"mailto:histonet-bounces@lists.u 79. 3D"mailto:histonet-bounces@lists 80. 3D"mailto:histonet@ 81. 3D"mailto:cthornton@dahlchase.com" 82. 3D"mailto:Histonet@lists.utsouthwestern.e 83. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/hi 84. file://localhost/tmp/3D"mai 85. 3D"mailto:histonet@lists.utsouthwestern.ed 86. file://localhost/tmp/3D"mai 87. 3D"mailto:SEsparza@seton.org" 88. 3D"mailto:dakshnapriya@neo.ta 89. file://localhost/tmp/3D"mai 90. ="mailto:histonet-bounces@lists.utsouthwestern.edu" 91. file://localhost/tmp/3D 92. 3D"mailto:histonet@lists.utsouthwestern.edu" 93. 3D"mailto:Histonet@lists.utsouthwes 94. 3D"http://lists.utsouthwestern.edu/mailman/listinf 95. 3D"mailto:dakshnapriya@neo.tamu.edu" 96. 3D"mailto:king.laurie@marshfieldclinic.org" 97. 3D"mailto:788819.JavaMail.root@neo. 98. file://localhost/tmp/3D"mailt 99. file://localhost/tmp/3D"mailt 100. 3D"mailto:h 101. 3D"mailto:histonet@lists.utsouthwestern.edu" 102. 3D"mailto:Histonet@lists.utsouthwestern.edu" 103. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 104. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From AGleiberman <@t> buffalobiolabs.com Wed May 21 09:23:06 2014 From: AGleiberman <@t> buffalobiolabs.com (Anatoli Gleiberman) Date: Wed May 21 09:23:12 2014 Subject: [Histonet] HA-tag antibody In-Reply-To: References: Message-ID: HI Eva, Goat anti-HA antibody from Abcam supposed to work on FFPE sections: http://www.abcam.com/ha-tag-antibody-ab94918.html Don't know working dilution. Rabbit antibody from Abcam should probably work as well - I tried them only on formalin-fixed cryo-sections: http://www.abcam.com/ha-tag-antibody-chip-grade-ab9110.html I used it in dilution 1:200 for immunofluorescence. Chicken anti-HA antibody from Aves (cat.#ET-HA100) should work as well: http://www.aveslab.com/products/epitope-tag-and-gfp-antibodies/anti-ha-hemagglutinin-antibodies/ Should work in dilution 1:500-1:1000 for immunofluorescence. Anatoli Gleiberman, Ph.D. Director of Histopathology Buffalo Biolabs LLC 73 High Street Buffalo, NY 14203 Phone: 716-849-6810x354 e-mail: agleiberman@buffalobiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Wednesday, May 21, 2014 9:54 AM To: histonet Subject: [Histonet] HA-tag antibody Hello, I am looking for an HA-tag antibody to detect a protein labeled with HA in formalin fixed paraffin embedded tissues. Could anyone suggest a published antibody? The tissue of interest is mouse so I would prefer an antibody made in another species. I have found several antibodies but can't find them published. Thank you, Eva Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed May 21 10:29:31 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed May 21 10:29:50 2014 Subject: [Histonet] HA-tag antibody In-Reply-To: References: Message-ID: <1023073911.12256864.1400686171048.JavaMail.root@comcast.net> Eva, Not sure you are going to find that much "published" on HA-tag antibodies.? That 9 amino acid sequence used by most to tag; people are looking to identify the fusion partner of the tag and worry little about publishing on the tag itself.? Same with the famous 8 amino acid sequence FLAG-tag.? I agree with Anatoli Gleiberman with what he recommended.? Also look at Cell Signalling; they have non-mouse antibodies to HA-tag for paraffin and have some pictures.? But few people are concerned with publishing on a tag itself but with the partner in the fusion that they are after.? Used HA and FLAG tags in paraffin often but that wasn't any publishable finding.? Beware if your mouse has influenza in being sure what you are seeing. ? Ray Koelling Seattle, WA ----- Original Message ----- From: "Eva Permaul" To: "histonet" Sent: Wednesday, May 21, 2014 6:53:51 AM Subject: [Histonet] HA-tag antibody Hello, I am looking for an HA-tag antibody to detect a protein labeled with HA in formalin fixed paraffin embedded tissues. Could anyone suggest a published antibody? The tissue of interest is mouse so I would prefer an antibody made in another species. I have found several antibodies but can't find them published. Thank you, Eva Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JPitts <@t> dermatologyprofessionals.com Wed May 21 10:52:54 2014 From: JPitts <@t> dermatologyprofessionals.com (Jackie Pitts) Date: Wed May 21 10:52:59 2014 Subject: [Histonet] lecia heme 560 mx Message-ID: <85F6442EC5759B48900C8454E873373102244A8E7C07@SBS01.dermpro.local> Hi, I have some samples of the Leica heme 560 mx and was wondering if anyone had a protocol I could try so I can try it out. Thanks! Jackie Heitz, HT(ASCP)CM 218-454-3520 Dermatology Professionals, PA Baxter, MN 56425 From dthorek1 <@t> jhmi.edu Wed May 21 13:32:55 2014 From: dthorek1 <@t> jhmi.edu (Daniel Thorek) Date: Wed May 21 13:33:33 2014 Subject: [Histonet] CN Knife Holder for CM1800/1850 Message-ID: Hello, I was hoping that someone might have an older Leica CN Knife holder that fits the CM1800 or CM1850 systems that they are not using. I have a legacy cryostat that I am going to try and get up and running for bone sectioning. Specifically I will be looking at sectioning un-decalcified mouse tibia. Does anyone also have suggestions for cryotape transferring? Thank you all. Daniel L J Thorek, PhD. dthorek1@jhmi.edu Division of Nuclear Medicine Department of Radiology and Radiological Sciences The Johns Hopkins School of Medicine From clemenma <@t> evms.edu Wed May 21 14:16:27 2014 From: clemenma <@t> evms.edu (Clements, Mary Ann) Date: Wed May 21 14:16:38 2014 Subject: [Histonet] Tissue Tek VIP 5 cleaning cycle Message-ID: <7C3ADBB9F8E8E442B69BDE04519091DB0C85E711@Trillya.evms.net> Please excuse my ignorance as I am new to the world of Histology! We currently run a 96 minute cleaning cycle after processing only 10-30 blocks. Is it feasible to run a 60 minute cleaning cycle since our volume is so low or is length of cleaning cycle independent of block count? Thank you in advance for your expertise, Mary Ann Clements, B.S. Biorepository Manager Eastern Virginia Medical School Leroy T. Canoles Jr. Cancer Research Center Lewis Hall, Room 3018 700 West Olney Road Norfolk, VA 23507 email: clemenma@evms.edu phone: (757) 446-7910 fax: (757) 446- 7059 From Bruce_Palmatier <@t> vwr.com Wed May 21 15:01:01 2014 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Wed May 21 15:01:27 2014 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 05/23/2014) Message-ID: I am out of the office until 05/23/2014. I will be out of the office on business travel from May 19th through May 23rd. I will have limited access to email. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 126, Issue 20" sent on 5/21/2014 1:58:38 PM. This is the only notification you will receive while this person is away. From algranth <@t> email.arizona.edu Wed May 21 15:22:53 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed May 21 15:23:09 2014 Subject: [Histonet] RE: Tissue Tek VIP 5 cleaning cycle In-Reply-To: <7C3ADBB9F8E8E442B69BDE04519091DB0C85E711@Trillya.evms.net> References: <7C3ADBB9F8E8E442B69BDE04519091DB0C85E711@Trillya.evms.net> Message-ID: The cleaning cycle is not dependent on the number of blocks you process. Even if you process one block the processor has sucked up all the reagents and the paraffin into the retorty and has to go thru a clean cycle to clean out the lines and the valves to prevent them from getting a paraffin clog. The VIP has a built in minimum amount of times that the xylene and the alcohol (and water if you use it after the alcohol) have to get sucked up into the retort and spit out through the lines and valves. This is to make sure any paraffin residue is dissolved. This is also the reason the VIP alerts you after every five clean cycles so that you can change the solutions because they may be saturated with paraffin and could leave some behind in the lines. Trust me - you do not want to clog up your processor. Not fun to clean it out. I, myself, have never done that but in another life I was in sales and I saw some awful things that people did to their processors. Andi ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Clements, Mary Ann [clemenma@evms.edu] Sent: Wednesday, May 21, 2014 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Tek VIP 5 cleaning cycle Please excuse my ignorance as I am new to the world of Histology! We currently run a 96 minute cleaning cycle after processing only 10-30 blocks. Is it feasible to run a 60 minute cleaning cycle since our volume is so low or is length of cleaning cycle independent of block count? Thank you in advance for your expertise, Mary Ann Clements, B.S. Biorepository Manager Eastern Virginia Medical School Leroy T. Canoles Jr. Cancer Research Center Lewis Hall, Room 3018 700 West Olney Road Norfolk, VA 23507 email: clemenma@evms.edu phone: (757) 446-7910 fax: (757) 446- 7059 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RHEYNA <@t> lumc.edu Wed May 21 17:40:45 2014 From: RHEYNA <@t> lumc.edu (ROGER HEYNA) Date: Wed May 21 17:40:52 2014 Subject: [Histonet] unsubscribe Message-ID: Please unsubscribe From Susan.Walzer <@t> HCAHealthcare.com Thu May 22 02:00:15 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Thu May 22 02:00:27 2014 Subject: [Histonet] RE: Tissue Tek VIP 5 cleaning cycle In-Reply-To: References: <7C3ADBB9F8E8E442B69BDE04519091DB0C85E711@Trillya.evms.net> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FBA98D517@FWDCWPMSGCMS09.hca.corpad.net> Amen! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, May 21, 2014 4:23 PM To: Clements, Mary Ann; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Tek VIP 5 cleaning cycle The cleaning cycle is not dependent on the number of blocks you process. Even if you process one block the processor has sucked up all the reagents and the paraffin into the retorty and has to go thru a clean cycle to clean out the lines and the valves to prevent them from getting a paraffin clog. The VIP has a built in minimum amount of times that the xylene and the alcohol (and water if you use it after the alcohol) have to get sucked up into the retort and spit out through the lines and valves. This is to make sure any paraffin residue is dissolved. This is also the reason the VIP alerts you after every five clean cycles so that you can change the solutions because they may be saturated with paraffin and could leave some behind in the lines. Trust me - you do not want to clog up your processor. Not fun to clean it out. I, myself, have never done that but in another life I was in sales and I saw some awful things that people did to their processors. Andi ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Clements, Mary Ann [clemenma@evms.edu] Sent: Wednesday, May 21, 2014 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Tek VIP 5 cleaning cycle Please excuse my ignorance as I am new to the world of Histology! We currently run a 96 minute cleaning cycle after processing only 10-30 blocks. Is it feasible to run a 60 minute cleaning cycle since our volume is so low or is length of cleaning cycle independent of block count? Thank you in advance for your expertise, Mary Ann Clements, B.S. Biorepository Manager Eastern Virginia Medical School Leroy T. Canoles Jr. Cancer Research Center Lewis Hall, Room 3018 700 West Olney Road Norfolk, VA 23507 email: clemenma@evms.edu phone: (757) 446-7910 fax: (757) 446- 7059 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bethcoxx <@t> gmail.com Thu May 22 05:41:09 2014 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Thu May 22 05:41:01 2014 Subject: [Histonet] RE: HTL Certification Message-ID: <537DD445.7050107@gmail.com> Hi Cecy, I recommend the Michigan Society of Histotech's HT/HTL Study Workbook. This will take you through everything you need for the HTL in an organized and clear manner (much better than the ASCP outline). Note that this is a workbook that you fill in, not a question/answer book. It has forms and charts to lead you through all your study. It will help you understand the info, much better than just memorizing. I think the cost is $25. you can order one at: www.mihisto.org Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech _____________________________________________________________________________________ Message: 1 Date: Mon, 19 May 2014 12:32:36 -0500 From: Cecy Stan Subject: [Histonet] HTL certification To:histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hello everyone, I'm starting to prepare for my HTL certification (I am very nervous and anxious but also very excited about this decision to go for it). I was just curious to know how you guys prepared for it, and how long it took for you to prepare before taking the test. Will 6 months preparation be enough? (I know that may depend on the individual; it's just that I had my Masters over 10 years ago and I haven't studied this much since then). I have Freida L. Carson's 3rd Edition book -- quite daunting to memorize -- but the outline ASCP has provided for study seems to be helpful. Do you have any other book/study aid suggestions? Thank you in advance for your input and advice! C.A. From Joyce.Weems <@t> emoryhealthcare.org Thu May 22 07:20:52 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu May 22 07:21:10 2014 Subject: [Histonet] RE: unsubscribe In-Reply-To: References: Message-ID: You must do that yourself at this web site.. http://lists.utsouthwestern.edu/mailman/listinfo/histonet Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ROGER HEYNA Sent: Wednesday, May 21, 2014 6:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] unsubscribe Please unsubscribe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Kathy.Machado <@t> LPNT.net Thu May 22 09:17:18 2014 From: Kathy.Machado <@t> LPNT.net (Kathy.Machado@LPNT.net) Date: Thu May 22 09:17:58 2014 Subject: [Histonet] RE: antibody vials Message-ID: I use my old racks from the Dako Autostainer! They hold the Dako vials nicely and some of the smaller ones! Kathy Machado,HTL Danville Regional Medical Center Danville, VA From algranth <@t> email.arizona.edu Thu May 22 11:23:08 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu May 22 11:23:16 2014 Subject: [Histonet] Re: Tissue Tek VIP 5 cleaning cycle In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FBA98D517@FWDCWPMSGCMS09.hca.corpad.net> References: <7C3ADBB9F8E8E442B69BDE04519091DB0C85E711@Trillya.evms.net> <4BF03F5404EBDE409AF9232DA74B9DED2FBA98D517@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <52421DCD-3645-421A-B11F-3240080EA821@email.arizona.edu> Just a coincidence - I was working in another lab this week and when I was cleaning out the processor (not a VIP) after a cleaning cycle I was picking out chunks of paraffin. I asked the tech (not a histotech) when the last time was that the cleaning reagents were changed and she said, "December". OMG! I did my best to hold back any comments but I changed out the xylene and alcohol as soon as I could. Then I said I thought the reagents need to be changed more often. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From clemenma <@t> evms.edu Thu May 22 11:24:04 2014 From: clemenma <@t> evms.edu (Clements, Mary Ann) Date: Thu May 22 11:24:12 2014 Subject: [Histonet] RE: Tissue Tek VIP 5 cleaning cycle In-Reply-To: <52421DCD-3645-421A-B11F-3240080EA821@email.arizona.edu> References: <7C3ADBB9F8E8E442B69BDE04519091DB0C85E711@Trillya.evms.net> <4BF03F5404EBDE409AF9232DA74B9DED2FBA98D517@FWDCWPMSGCMS09.hca.corpad.net> <52421DCD-3645-421A-B11F-3240080EA821@email.arizona.edu> Message-ID: <7C3ADBB9F8E8E442B69BDE04519091DB0C85FA0C@Trillya.evms.net> YIKES!! Mary Ann Clements, B.S. Biorepository Manager Eastern Virginia Medical School Leroy T. Canoles Jr. Cancer Research Center Lewis Hall, Room 3018 700 West Olney Road Norfolk, VA 23507 email: clemenma@evms.edu phone: (757) 446-7910 fax: (757) 446- 7059 From: Grantham, Andrea L - (algranth) [mailto:algranth@email.arizona.edu] Sent: Thursday, May 22, 2014 12:23 PM To: Cc: Clements, Mary Ann; Subject: Re: Tissue Tek VIP 5 cleaning cycle Just a coincidence - I was working in another lab this week and when I was cleaning out the processor (not a VIP) after a cleaning cycle I was picking out chunks of paraffin. I asked the tech (not a histotech) when the last time was that the cleaning reagents were changed and she said, "December". OMG! I did my best to hold back any comments but I changed out the xylene and alcohol as soon as I could. Then I said I thought the reagents need to be changed more often. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From leila.etemadi <@t> med.lu.se Fri May 23 00:58:58 2014 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Fri May 23 00:59:16 2014 Subject: [Histonet] imageJ Message-ID: <209025AC-6D3A-429F-AA95-9AE02021AFE3@med.lu.se> Good morning all Histoneter, I was wonder if any one out there knows where I can download ITCN plugin version 1.6 for counting cells?, I can?t find a proper page for downloading it! Terribly sorry if my question is irrelevant! Thanks a lot for help and support as always. Cheers, Leila :-) From estellamireles <@t> gmail.com Fri May 23 09:13:08 2014 From: estellamireles <@t> gmail.com (Stella Mireles) Date: Fri May 23 09:13:11 2014 Subject: [Histonet] Hard Tissue Lab Message-ID: Does anyone know of a hard tissue lab that will work on outside cases? A doctor I know needs this information. Thanks *Courage without limits. Thank you to all whose loved ones fought and served for us. I will remember them. * From pruegg <@t> ihctech.net Fri May 23 11:14:14 2014 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri May 23 11:14:18 2014 Subject: [Histonet] FW: IHC training In-Reply-To: References: , , Message-ID: From: pruegg@ihctech.net To: pruegg@ihctech.net Subject: FW: IHC training Date: Fri, 23 May 2014 09:52:27 -0600 Patsy Ruegg pruegghm@hotmail.com or pruegg@ihctech.net Can anyone help Mrs. Lawal coming from Nigeria to the NSH S/C in Austin in Aug this year? She wants to visit a lab for some more IHC training. We tried to find a lab in the Austin area but failed. We were going to let her come to U of Colorado but the timing is not good for them. I will post this to histonet and the NSH FB group site as well. Mrs. Lawal's email addresses are listed below. ojadesola@yahoo.com Message from Eve Kemble: This is Eve from the IHCRG. I am at Dartmouth Hitchcock Medical Center in NH, and I have a question for you: I met Mrs. Lawal in Vancouver at the NSH S/C in 2012. She was one of the keynote speakers at that meeting.Mrs. Lawal is in charge of a histology lab at the Lagos University Teaching Hospital in Nigeria.She currently uses a Ventana stainer to do the IHC in her lab.Mrs. Lawal is planning to attend the S/C in Austin, and would like to stay on for a while after the S/C so that she could visit another lab for some further practical training and to observe how things are done elsewhere.Can you recommend any lab/s that would be willing to host Mrs. Lawal? Mrs. Lawal is very knowledgeable in IHC and is very keen in improving the processes that are done in her lab. Any information will be most welcome. Many thanks for your help! Cheers, Eve Kemble. eve.kemble@gmail.com Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From smah2 <@t> msn.com Tue May 27 10:41:26 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Tue May 27 10:41:35 2014 Subject: [Histonet] Sakura Cryostat Message-ID: Hi all! Is anyone using the Sakura Cryo 3 Plus cryostat for routine frozen sections? A nearby facility is inquiring but I have no experience with it myself. Any opinions would be appreciated. Thank you, Sheila Haas MicroPath Laboratories, Inc. From juliana.montero <@t> usmd.com Tue May 27 10:50:47 2014 From: juliana.montero <@t> usmd.com (Juliana Montero) Date: Tue May 27 10:51:31 2014 Subject: [Histonet] FiSH Message-ID: <80CC962B464A9D40A226C5B3D252DCD02DA9974C@S1P5DAG3E.EXCHPROD.USA.NET> Does anyone use PersonalizeDx for FiSH specimens? We recently started using them and I have some questions. Juliana Montero, HTL Lead Histotechnologist ________________________________ USMD Diagnostics Services, LLC Pathology Lab 900 Airport Fwy, Hurst, TX 76054 Office: (817) 576-0590 Fax: (817) 576-0591 The information contained in this e-mail message is intended exclusively for the recipient(s) named above. This communication may contain information that is confidential, proprietary or otherwise legally exempt from disclosure. If you are not the named addressee, you are not authorized to read, print, retain, copy or disseminate this message or any part of it.If you have received this message in error, please notify the sender immediately by e-mail and delete all copies of the message. Review by any individual other than the intended recipient does not waive a privileged relationship (if applicable) between the sender and the recipient. From tissuetech <@t> juno.com Wed May 28 08:39:31 2014 From: tissuetech <@t> juno.com (tissuetech@juno.com) Date: Wed May 28 08:41:19 2014 Subject: [Histonet] Histotech Needed Message-ID: <20140528.083931.3414.0@webmail10.vgs.untd.com> Hi Everyone; Small private (Fun, Upbeat) reference lab in Dallas, Texas seeks a certified Histotech to be part of there Team. This individual should be energetic, upbeat & capable of working on own when necessary and able to help lead other Team members when necessary. If you feel that you qualify and would like to be part of a Team that is valued, then you should send your Resume to (tissuetech@juno.com). ____________________________________________________________ Do THIS before eating carbs (every time) 1 EASY tip to increase fat-burning, lower blood sugar & decrease fat storage http://thirdpartyoffers.juno.com/TGL3141/5385e74927ee67480569st01vuc From GenieJacobs <@t> texashealth.org Wed May 28 12:20:17 2014 From: GenieJacobs <@t> texashealth.org (Jacobs, Genie) Date: Wed May 28 12:20:24 2014 Subject: [Histonet] RE: Histonet Digest, Vol 126, Issue 26 In-Reply-To: References: Message-ID: MD Pathology Plano, TX-Looking for Histotech with background in Immunos for full-time position Please call Genie at 972-981-3108 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Wednesday, May 28, 2014 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 126, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Histotech Needed (tissuetech@juno.com) ---------------------------------------------------------------------- Message: 1 Date: Wed, 28 May 2014 13:39:31 GMT From: "tissuetech@juno.com" Subject: [Histonet] Histotech Needed To: histonet@lists.utsouthwestern.edu Message-ID: <20140528.083931.3414.0@webmail10.vgs.untd.com> Content-Type: text/plain; charset=windows-1252 Hi Everyone; Small private (Fun, Upbeat) reference lab in Dallas, Texas seeks a certified Histotech to be part of there Team. This individual should be energetic, upbeat & capable of working on own when necessary and able to help lead other Team members when necessary. If you feel that you qualify and would like to be part of a Team that is valued, then you should send your Resume to (tissuetech@juno.com). ____________________________________________________________ Do THIS before eating carbs (every time) 1 EASY tip to increase fat-burning, lower blood sugar & decrease fat storage http://thirdpartyoffers.juno.com/TGL3141/5385e74927ee67480569st01vuc ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 126, Issue 26 ***************************************** The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From mike <@t> dbiosys.com Wed May 28 12:43:08 2014 From: mike <@t> dbiosys.com (Michael Thompson) Date: Wed May 28 12:43:36 2014 Subject: [Histonet] FW: IHC training Message-ID: <90q7rjyo7jsjmpbn0u2vgx20.1401298988405@email.android.com> DBS is willing to train her at NSH. We can go over hand staining and our autostainer. Michael Michael Thompson Dir of Sales Diagnostic BioSystems 4128601288 www.dbiosys.com "IHC Made Affordable" Patsy Ruegg wrote: > > > > >From: pruegg@ihctech.net >To: pruegg@ihctech.net >Subject: FW: IHC training >Date: Fri, 23 May 2014 09:52:27 -0600 > >Patsy Ruegg pruegghm@hotmail.com or pruegg@ihctech.net > > > > >Can anyone help Mrs. Lawal coming from Nigeria to the NSH S/C in Austin in Aug this year? She wants to visit a lab for some more IHC training. We tried to find a lab in the Austin area but failed. We were going to let her come to U of Colorado but the timing is not good for them. I will post this to histonet and the NSH FB group site as well. Mrs. Lawal's email addresses are listed below. > >ojadesola@yahoo.com > > >Message from Eve Kemble: > > >This is Eve from the IHCRG. I am at Dartmouth Hitchcock Medical Center in NH, and I have a question for you: I met Mrs. Lawal in Vancouver at the NSH S/C in 2012. She was one of the keynote speakers at that meeting.Mrs. Lawal is in charge of a histology lab at the Lagos University Teaching Hospital in Nigeria.She currently uses a Ventana stainer to do the IHC in her lab.Mrs. > Lawal is planning to attend the S/C in Austin, and would like to stay >on for a while after the S/C so that she could visit another lab for >some further practical training and to observe how things are done elsewhere.Can you recommend any lab/s that would be willing to host Mrs. Lawal? Mrs. Lawal is very knowledgeable in IHC and is very keen in improving the processes that are done in her lab. Any information will be most welcome. Many thanks for your help! Cheers, Eve Kemble. >eve.kemble@gmail.com >Patsy Ruegg, HT(ASCP)QIHC >Ruegg IHC Consulting >40864 E Arkansas Ave >Bennett, CO 80102 >H 303-644-4538 >C 720-281-5406 >pruegghm@hotmail.com >pruegg@ihctech.net > > > > > > > > > > _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> pathlab.us Wed May 28 12:45:25 2014 From: histo <@t> pathlab.us (Histology) Date: Wed May 28 12:45:57 2014 Subject: [Histonet] Sentinel nodes Message-ID: <566E7795BC94204D9A21539DE282AED7099BFC@DC01.dp.local> Hi All, Sorry, if you already responded to this. We had computer issues and I missed some emails. I am hoping that someone would share their policy/procedures on sentinel lymph nodes. In particular, documenting that all staff and couriers know that they may be exposed to low levels of radiation. Thanks in advance! From igor.deyneko <@t> gmail.com Wed May 28 17:35:33 2014 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Wed May 28 17:35:39 2014 Subject: [Histonet] Jobs for MBAs Message-ID: Dear Histonetters, I am wondering if there are any job opportunities for someone with MBA in Marketing & Operations? I'm a former scientists and was a Histotech, managed a lab and developed from scratch a histology core facility at a medium size pharma company. I went to get an MBA and finishing soon and want to return into science but from Business side. So, wondering if there are any potential opportunities in Histotechnology for MBAs. I would appreciate any leads and advice. Thank you very much. Igor Deyneko D'Amore - McKim School Of Business Northeastern University 360 Huntington Avenue, Boston, 02116 From joelleweaver <@t> hotmail.com Wed May 28 17:43:11 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed May 28 17:43:15 2014 Subject: [Histonet] Jobs for MBAs In-Reply-To: References: Message-ID: Think about lab operations, account manager for a vendor, there are some research and pharma companies with various levels of management, office -practice management, if you are also good with IT, LIS analyst, manager of analysts, business analyst, implementation specialist ( LIS vendors), various types of consulting-J These are just off the top of my head, but the lab is a business and we need people who understand the clinical, the business & operations, sales & markets. There are probably many options out there depending on your specific experience, interests and inclinations. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 28 May 2014 18:35:33 -0400 > From: igor.deyneko@gmail.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Jobs for MBAs > > Dear Histonetters, > > I am wondering if there are any job opportunities for someone with MBA in > Marketing & Operations? > I'm a former scientists and was a Histotech, managed a lab and developed > from scratch a histology core facility at a medium size pharma company. I > went to get an MBA and finishing soon and want to return into science but > from Business side. So, wondering if there are any potential opportunities > in Histotechnology for MBAs. > I would appreciate any leads and advice. > Thank you very much. > Igor Deyneko > D'Amore - McKim School Of Business > Northeastern University > 360 Huntington Avenue, > Boston, 02116 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 28 19:44:08 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 28 19:44:19 2014 Subject: [Histonet] Jobs for MBAs In-Reply-To: References: Message-ID: <1401324248.72208.YahooMailNeo@web120401.mail.ne1.yahoo.com> Try contacting one of those companies specializing in looking for?lab positions (like Relya). They may have some requests for lab administrators. Good luck Ren? J.?? On Wednesday, May 28, 2014 6:36 PM, Igor Deyneko wrote: Dear Histonetters, I am wondering if there are any job opportunities for someone with MBA in Marketing & Operations? I'm a former scientists and was a Histotech, managed a lab and developed from scratch a histology core facility at a medium size pharma company. I went to get an MBA and finishing soon and want to return into science but from Business side. So, wondering if there are any potential opportunities in Histotechnology for MBAs. I would appreciate any leads and advice. Thank you very much. Igor Deyneko D'Amore - McKim School Of Business Northeastern University 360 Huntington Avenue, Boston, 02116 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sccrshlly <@t> yahoo.com Thu May 29 09:38:22 2014 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Thu May 29 09:41:30 2014 Subject: [Histonet] Histotech Needed in Austin, TX Message-ID: <1401374302.29367.YahooMailNeo@web162301.mail.bf1.yahoo.com> Busy GI pathology laboratory in beautiful North Austin area seeking histotech for permanent, full-time position.? The successful candidate will have a current ASCP certification (HT/HTL) or be registry eligible, a minimum of 2 years experience is preferred, and excellent histology skills. Job duties include receiving and accessioning specimens, performing basic histology tasks such as, but not limited to, processing, embedding, cutting, and staining GI biopsy specimens at the highest level of quality in an efficient manner, send out testing, and QA/QC tasks. Hours are M-F 6 AM to 2 PM.? No weekends and no on-call. Candidate must be customer service oriented and able to provide excellent service to the Gastroenterologists that we service as well as the pathologists that we work with. As a full time employee, we provide a competetive salary and excellent benefits package including access to medical, dental, life and disability coverages along with paid holidays, generous paid time off accruals, and excellent retirement benefits. ? Please email resumes to?Kelli Wood, HR?at ?AGcareers@austingastro.com. ? This is a job opening at the lab I work in.? Very laid back family-oriented atmosphere :) From relia1 <@t> earthlink.net Thu May 29 10:17:42 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu May 29 10:17:51 2014 Subject: [Histonet] RELIA HOT JOB ALERT - IHC Specialist needed in San Francisco Bay Area. A RELIA EXCLUSIVE!! Message-ID: <00f701cf7b51$2350d0a0$69f271e0$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day during this short week!! I thought I would post this position here on the histonet because it is a great opportunity and it is not being advertised on any job boards. If you are interested please contact me. If you know someone who is interested I will pay a referral fee if I place them. RELIA HOT HISTOLOGY JOB ALERT!! Immunohistochemistry QC Manager - San Francisco, CA A RELIA EXCLUSIVE RELIA Solutions has been engaged exclusively and confidentially by an elite well known supplier to the laboratory field to assist in their search for an IHC/QC Manager. Exceptional in depth knowledge of immunohistochemistry and quality assurance and controls is essential. ASCP HT/HTL/QIHC, BS and 5-7 years experience are preferred. In return my client offers competitive compensation, an exceptional environment and an outstanding group of people to work with. For more information contact Pam Barker at relia1@earthlink.net or 407-353-5070 Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tbraud <@t> holyredeemer.com Thu May 29 14:37:05 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu May 29 14:37:11 2014 Subject: [Histonet] Sentinel node policy In-Reply-To: <20140529161557.667061E806E@trendmess-svr.holyredeemer.local> References: <20140529161557.667061E806E@trendmess-svr.holyredeemer.local> Message-ID: I'd be happy to share my policy, but it contains nothing about low level exposure to staff. The levels to which staff and couriers are exposed, is approximate to naturally occurring background radiation. They probably would have a higher exposure level wearing a watch with glow in the dark dial. Why stress a problem when there is none? I hope this helps. Here's some documentation that I've used for my procedure: Am J Surg. 1999 Dec;178(6):454-7. Radiation safety with breast sentinel node biopsy. Stratmann SL1, McCarty TM, Kuhn JA. "Radiation exposure to operating room personnel, pathologists, and operative equipment during a breast sentinel node biopsy using Tc99m is minimal. A primary surgeon can perform 2,190 hours, a scrub nurse 33,333 hours, and a pathologist 14,705 hours of procedural work before surpassing Occupational Safety and Health Administration limits. Operative instruments, pathology slides, and cryostat machines do not require special handling" Eur J Nucl Med. 2000 Apr;27(4):377-91. Radiation safety of the sentinel lymph node technique in breast cancer. Waddington WA1, Keshtgar MR, Taylor I, Lakhani SR, Short MD, Ell PJ. "The radiation doses to staff groups involved in all aspects of the technique are low, and under normal circumstances and levels of workload, routine radiation monitoring will not be required" Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 3. Sentinel nodes (Histology) Message: 3 Date: Wed, 28 May 2014 17:45:25 +0000 From: Histology Subject: [Histonet] Sentinel nodes Hi All, Sorry, if you already responded to this. We had computer issues and I missed some emails. I am hoping that someone would share their policy/procedures on sentinel lymph nodes. In particular, documenting that all staff and couriers know that they may be exposed to low levels of radiation. Thanks in advance! ************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From bethany <@t> mydermsurgery.com Thu May 29 15:22:36 2014 From: bethany <@t> mydermsurgery.com (Bethany) Date: Thu May 29 15:22:41 2014 Subject: [Histonet] (no subject) Message-ID: <667714489.199441.1401394956875.open-xchange@oxuslxltgw03.lxa.perfora.net> The doctor I work for wants to use Mart 1 staining for Mohs on melanoma cases. Any information on where to start? What equipment do we need? Where do we acquire the stains/antibodies, etc? thx Bethany From rsrichmond <@t> gmail.com Thu May 29 18:47:57 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu May 29 18:48:01 2014 Subject: [Histonet] Re: Sentinel nodes Message-ID: An anonymous inquirer asks: >>I am hoping that someone would share their policy/procedures on sentinel lymph nodes. In particular, documenting that all staff and couriers know that they may be exposed to low levels of radiation.<< Sentinel lymph nodes, and the associated lumpectomy specimens, contain a small amount of radioactive material, technetium 99m sulfur colloid. Gamma emitter 99Tc has a half-life of only six hours, and exposes you to a negligible amount of radiation - you could eat the patient's entire dose and it wouldn't do you harm. The histotechnologist and the pathologist do not need any special precautions to handle this material - in particular, there's no need to delay processing it for several days. All of this has been pretty much standard procedure for a good many years. I've never seen any warning issued that the material is radioactive, and I've seen a considerable number of them in many institutions and in several US states. If you're dealing with a local bureaucratic requirement, you could challenge it. Bob Richmond Samurai Pathologist Maryville TN From amurvosh <@t> advancederm.net Fri May 30 08:18:33 2014 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Fri May 30 08:18:44 2014 Subject: [Histonet] (no subject) In-Reply-To: <667714489.199441.1401394956875.open-xchange@oxuslxltgw03.lxa.perfora.net> References: <667714489.199441.1401394956875.open-xchange@oxuslxltgw03.lxa.perfora.net> Message-ID: <4AD6A4E531E8C943A730559B6B81DF07DA8F64@dc.Advancederm.net> I just got back from the Mohs conference. The Biocare people have the stain and told me they would come out to help work it up. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bethany Sent: Thursday, May 29, 2014 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) The doctor I work for wants to use Mart 1 staining for Mohs on melanoma cases. Any information on where to start? What equipment do we need? Where do we acquire the stains/antibodies, etc? thx Bethany _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmjohnson34 <@t> hotmail.com Fri May 30 10:17:25 2014 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Fri May 30 10:17:32 2014 Subject: [Histonet] Is there a Pathology billing hotline? Message-ID: Today we received one container labelled 'sentinel nodes' for frozen section. There were five nodes and they were placed in two chucks. How in the heck are we to know how to code for charges for this? Is it per container, per node, or per frozen? Plus, is there a person or agency that you can call to ask these questions? Thanks, Jennifer Johnson Sent from Jenny's iPhone From Joyce.Weems <@t> emoryhealthcare.org Fri May 30 10:44:54 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri May 30 10:45:09 2014 Subject: [Histonet] Is there a Pathology billing hotline? In-Reply-To: References: Message-ID: This would be one specimen, since the nodes were not separately identified. You would charge an 88331 for the first block and 88332 for the second block. (To the best of my knowledge!!) Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Friday, May 30, 2014 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Is there a Pathology billing hotline? Today we received one container labelled 'sentinel nodes' for frozen section. There were five nodes and they were placed in two chucks. How in the heck are we to know how to code for charges for this? Is it per container, per node, or per frozen? Plus, is there a person or agency that you can call to ask these questions? Thanks, Jennifer Johnson Sent from Jenny's iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From tbraud <@t> holyredeemer.com Fri May 30 13:50:34 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri May 30 13:50:39 2014 Subject: [Histonet] RE: Path Billing hotline In-Reply-To: <20140530161559.745721E8080@trendmess-svr.holyredeemer.local> References: <20140530161559.745721E8080@trendmess-svr.holyredeemer.local> Message-ID: Don't forget to add the 88307 charge for the specimen to the frozen charges (88331, 88332) Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 5. Is there a Pathology billing hotline? (Jennifer Johnson) 6. RE: Is there a Pathology billing hotline? (Weems, Joyce K.) Message: 5 Date: Fri, 30 May 2014 11:17:25 -0400 From: Jennifer Johnson Subject: [Histonet] Is there a Pathology billing hotline? Today we received one container labelled 'sentinel nodes' for frozen section. There were five nodes and they were placed in two chucks. How in the heck are we to know how to code for charges for this? Is it per container, per node, or per frozen? Plus, is there a person or agency that you can call to ask these questions? Thanks, Jennifer Johnson Sent from Jenny's iPhone ------------------------------ Message: 6 Date: Fri, 30 May 2014 15:44:54 +0000 From: "Weems, Joyce K." Subject: RE: [Histonet] Is there a Pathology billing hotline? This would be one specimen, since the nodes were not separately identified. You would charge an 88331 for the first block and 88332 for the second block. (To the best of my knowledge!!) Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org *********************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From rsrichmond <@t> gmail.com Fri May 30 16:57:40 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri May 30 16:57:44 2014 Subject: [Histonet] Re: Is there a Pathology billing hotline? Message-ID: Jennifer Johnson asks: >>Today we received one container labelled 'sentinel nodes' for frozen section. There were five nodes and they were placed in two chucks. How in the heck are we to know how to code for charges for this? Is it per container, per node, or per frozen? Plus, is there a person or agency that you can call to ask these questions?<< and Joyce Weems replies: >>This would be one specimen, [if] the nodes were not separately identified. You would charge an 88331 for the first block and 88332 for the second block.<< If the lymph nodes were separately identified, say, "sentinel node #1" through "sentinel node #5" then if you separated them from each other (say by marking them with different colored inks) then you could bill for five specimens, five frozen section codes 88331 and five specimen codes 88305. The final pathology report would have to identify them, say, 1. left axillary lymph node (sentinel node biopsy #1): Reactive hyperplasia. No malignant tumor identified. 2. - and so on through 5. Parenthetically, you shouldn't be doing frozen sections on grossly negative sentinel lymph nodes - it loses too much tissue. Touch preparations suffice. I don't know of any hot line for problems like this. Most of them are covered in the CAP Web site resource on the subject, if you can find it. It's quite chaotic, and it's worth reading it through and abstracting it on a slow day. These adjudications - which unfortunately are no longer done - are considered authoritative by the authorities. Here you read, for example, that a twin placenta is two codes 88307 iff you can identify cord A and cord B (doesn't matter how the identification is done). Bob Richmond Samurai Pathologist Maryville TN