[Histonet] IHC on animals

[Jan Shivers]

Dr. Raff,

You will need to do trial runs to first determine if the antibody can
detect the specific epitope in animal tissues (epitopes aren't always
conserved between species), and then do your own optimization to determine
the best dilution and tissue pretreatment for that particular species.
 They can vary by species, and don't always coincide with the
recommendations on the antibody data sheet provided by the vendor.

-- 
Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu



On Wed, Mar 26, 2014 at 3:03 PM, Lester Raff MD <LRaff <@t> uropartners.com>wrote:

> Hi:
>
>
>
> Can IHC validated for humans be performed on animal tissues without any
> protocol changes?
>
>
>
> Thanks,
>
>
>
> Lester J. Raff, MD MBA
>
> UroPartners
>
> Medical Director Of Laboratory
>
> 2225 Enterprise Dr. Suite 2511
>
> Westchester, Il 60154
>
> Tel: 708-486-0076
>
> Fax: 708-492-0203
>
>
>
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