[Histonet] Alizarin Red on undecalcified bone

[Mesru T]

Dear Histology Experts,


I need your help. I am trying to stain undecalcified bone (adult mouse
limb) with Alizarin Red S.
I was thinking about two options:
1. Fixed frozen sections with Cryojane tape transfer system then staining
the sections with Alizarin Red.
2. Alcohol fixed whole limb (whole mount preparation) stained with Alizarin
Red then decalcified and processed for paraffin embedding/sectioning. I am
not sure if the dye will survive decalcification and paraffin processing
procedures.
3. I am trying to avoid doing plastic embedding unless there is no other
option.

Any insights are highly appreciated.

Regards,
Mesru