From ratliffjack <@t> hotmail.com Sat Mar 1 03:16:50 2014 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Sat Mar 1 03:17:44 2014 Subject: [Histonet] Bone Histology Protocol In-Reply-To: <8ddec94ded8f4ad9bae1714af538db09@BL2PR01MB337.prod.exchangelabs.com> References: <8ddec94ded8f4ad9bae1714af538db09@BL2PR01MB337.prod.exchangelabs.com> Message-ID: Trevor, May I ask first if there is a particular reason why you are wanting to decalcify? Have you ever considered resin/plastic embedding of non-decalcified bone? Also, what type/species of bone are you wanting to process? My name is Jack Ratliff and I Chair the Hard Tissue Committee for the National Society for Histotechnology and as a professional histology organization and committee focusing on bone, biomaterials and medical device implants, we offer educational solutions to help those like yourself that are in search of information. This is accomplished through the presentation of workshops at the state, regional and national levels or even by providing free reference materials like processing manuals, books for purchase at non-member or member discounts, and free access to the archives of The Journal of Histotechnology to society members. If any of this interests you please check out the NSH website (www.nsh.org) where you can navigate around to view all of what the society has to offer, become a member and even connect with the Hard Tissue Committee. One last thing I can tell you now is that there will be several bone workshops available at the NSH Symposium/Convention this August in Austin, TX. I will also be one of the speakers at this meeting giving a workshop on resin embedding techniques in support of bone, biomaterials and medical device implants. Best Regards, Jack > On Feb 28, 2014, at 10:40 PM, "Wait, Trevor Jordan" wrote: > > Hello colleagues! I'm currently trying to construct a complete Bone Processing Protocol that includes fixation (10%NBF), decalcification (EDTA), Dehydration, Clearing of the decalcificant (Xylene), infiltration, and Embedding in Paraffin. I would like to look at some procedures just to get a good backbone of what a complete procedure is displayed like and I was hoping somebody could give me a website or a source where I could see some. I'm kind of new to bone histological processing so any procedures that are reliable will help! > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio > Class of 2017 MD Candidate > Abilene Christian University Class of 2013 Graduate > B.S. Biochemistry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From WaitT <@t> livemail.uthscsa.edu Sat Mar 1 08:22:06 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Sat Mar 1 08:22:14 2014 Subject: [Histonet] Bone Histology Protocol In-Reply-To: References: <8ddec94ded8f4ad9bae1714af538db09@BL2PR01MB337.prod.exchangelabs.com>, Message-ID: Thanks so much for the reference and I will visit that site and use that site most definitely as a resource. However, the reason I am wanting to decalcify is that my researcher (my employer) has instructed me that that is the way we will be doing it lol. I have read about resin/plastic embedding and that method does seem pretty efficient to use so I'm not sure why he opted to not go the plastic resin route. I do know that we have a limited budget to work with so maybe this is the cheapest route we can afford, along with a microtome that might not be sufficient to cut through hard bone and plastic. As far as the species of bone that we are processing, that too is something I'm not exactly sure about, but is something I should probably find out because from my readings, this is an important aspect. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________________ From: Jack Ratliff Sent: Saturday, March 1, 2014 3:16 AM To: Wait, Trevor Jordan Cc: histonet@lists.utsouthwestern.edu; Jack Ratliff Subject: Re: [Histonet] Bone Histology Protocol Trevor, May I ask first if there is a particular reason why you are wanting to decalcify? Have you ever considered resin/plastic embedding of non-decalcified bone? Also, what type/species of bone are you wanting to process? My name is Jack Ratliff and I Chair the Hard Tissue Committee for the National Society for Histotechnology and as a professional histology organization and committee focusing on bone, biomaterials and medical device implants, we offer educational solutions to help those like yourself that are in search of information. This is accomplished through the presentation of workshops at the state, regional and national levels or even by providing free reference materials like processing manuals, books for purchase at non-member or member discounts, and free access to the archives of The Journal of Histotechnology to society members. If any of this interests you please check out the NSH website (www.nsh.org) where you can navigate around to view all of what the society has to offer, become a member and even connect with the Hard Tissue Committee. One last thing I can tell you now is that there will be several bone workshops available at the NSH Symposium/Convention this August in Austin, TX. I will also be one of the speakers at this meeting giving a workshop on resin embedding techniques in support of bone, biomaterials and medical device implants. Best Regards, Jack > On Feb 28, 2014, at 10:40 PM, "Wait, Trevor Jordan" wrote: > > Hello colleagues! I'm currently trying to construct a complete Bone Processing Protocol that includes fixation (10%NBF), decalcification (EDTA), Dehydration, Clearing of the decalcificant (Xylene), infiltration, and Embedding in Paraffin. I would like to look at some procedures just to get a good backbone of what a complete procedure is displayed like and I was hoping somebody could give me a website or a source where I could see some. I'm kind of new to bone histological processing so any procedures that are reliable will help! > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio > Class of 2017 MD Candidate > Abilene Christian University Class of 2013 Graduate > B.S. Biochemistry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From WaitT <@t> livemail.uthscsa.edu Sat Mar 1 08:24:55 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Sat Mar 1 08:25:04 2014 Subject: [Histonet] Bone Histology Protocol In-Reply-To: References: <8ddec94ded8f4ad9bae1714af538db09@BL2PR01MB337.prod.exchangelabs.com>, Message-ID: <90060f2938974fdea3ce89006c2fb2ba@BL2PR01MB337.prod.exchangelabs.com> I'm terribly sorry, when you asked about species...my inclination was that you were curious which type of bone we were using (femur, humerus, tibia, etc. and cortical/cancellous) but we will be using human bone for this research project that has been donated from various orthopedic surgeries. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________________ From: Jack Ratliff Sent: Saturday, March 1, 2014 3:16 AM To: Wait, Trevor Jordan Cc: histonet@lists.utsouthwestern.edu; Jack Ratliff Subject: Re: [Histonet] Bone Histology Protocol Trevor, May I ask first if there is a particular reason why you are wanting to decalcify? Have you ever considered resin/plastic embedding of non-decalcified bone? Also, what type/species of bone are you wanting to process? My name is Jack Ratliff and I Chair the Hard Tissue Committee for the National Society for Histotechnology and as a professional histology organization and committee focusing on bone, biomaterials and medical device implants, we offer educational solutions to help those like yourself that are in search of information. This is accomplished through the presentation of workshops at the state, regional and national levels or even by providing free reference materials like processing manuals, books for purchase at non-member or member discounts, and free access to the archives of The Journal of Histotechnology to society members. If any of this interests you please check out the NSH website (www.nsh.org) where you can navigate around to view all of what the society has to offer, become a member and even connect with the Hard Tissue Committee. One last thing I can tell you now is that there will be several bone workshops available at the NSH Symposium/Convention this August in Austin, TX. I will also be one of the speakers at this meeting giving a workshop on resin embedding techniques in support of bone, biomaterials and medical device implants. Best Regards, Jack > On Feb 28, 2014, at 10:40 PM, "Wait, Trevor Jordan" wrote: > > Hello colleagues! I'm currently trying to construct a complete Bone Processing Protocol that includes fixation (10%NBF), decalcification (EDTA), Dehydration, Clearing of the decalcificant (Xylene), infiltration, and Embedding in Paraffin. I would like to look at some procedures just to get a good backbone of what a complete procedure is displayed like and I was hoping somebody could give me a website or a source where I could see some. I'm kind of new to bone histological processing so any procedures that are reliable will help! > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio > Class of 2017 MD Candidate > Abilene Christian University Class of 2013 Graduate > B.S. Biochemistry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From WaitT <@t> livemail.uthscsa.edu Sat Mar 1 22:15:18 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Sat Mar 1 22:15:25 2014 Subject: [Histonet] RE: Bone histotechniques In-Reply-To: <000001cf3569$2ae9b030$80bd1090$@bresnan.net> References: <000001cf3569$2ae9b030$80bd1090$@bresnan.net> Message-ID: <13034e43d1de46e28433a887fce3f33b@BL2PR01MB337.prod.exchangelabs.com> Thank you so much for the materials, they were definitely helpful for the EDTA and I very much appreciate that! Yes I have contacted my Researcher, unfortunately it is the weekend whenever I started to research for this protocol so my answers may not come til Monday morning. However, I do know that our species is human bones that have been donated from various orthopedic surgeries. As far as the exact type of bone (tibia, femur, etc.) I do not know the answer for they are just shavings or scraps that have been conserved from the surgeries that would otherwise be exposed of. I will also obtain that information as well. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: gayle callis Sent: Saturday, March 1, 2014 10:13 AM To: Wait, Trevor Jordan Subject: Bone histotechniques Trevor, Please say what kind of bone you are working with, including species, femur, tibia, etc. Attached is an excellent EDTA decalcification that was developed years ago by a bone expert/professor, and is published. Once you give more bone details, then I can expound on how to do this. You will probably get many replies. Also, are you using automated tissue processing or have to do this by hand? What are you to do for staining? Immunohistochemistry? Other staining only? There are a lot of variables here that affect how you fix, etc for the bone you are working with. Gayle Callis HTL/HT/MT(ASCP) From lpwenk <@t> sbcglobal.net Sun Mar 2 07:47:54 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Mar 2 07:47:57 2014 Subject: [Histonet] NSH Scholarships/Awards - June 1 deadline Message-ID: <23A422BA040A42888373100753FD0DF2@HP2010> Just wanted to remind people that NSH has $30,000 in scholarships and awards available to help people attend NSH educational symposiums, participate in NSH teleconferences, go to college, get histology/IHC/Mol path training at another institution, help NAACLS students, etc. Deadline for most is June 1, 2014 (NAACLS HT/HTL students deadline is March 15, 2014). For more information on how to apply (or to nominate someone), go to: http://www.nsh.org/scholarships-awards Peggy A. Wenk, HTL(ASCP)SLS From ibernard <@t> uab.edu Sun Mar 2 15:53:15 2014 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Sun Mar 2 15:53:19 2014 Subject: [Histonet] Best Study material for HTL Message-ID: Fellow Histonetters. I will sit for the HTL exam over next three to four months. Per the ASCP suggested list, the following study materials were suggested as study for the HTL. Any thoughts on narrowing the list of study materials or are all materials indicted necessary? * Carson, F. & Hladik, C. (2009). Histotechnology: A Self-Instructional Text (3rd ed.). Chicago: ASCP Press. * Kiernan, J. (2008). Histological and Histochemical Methods: Theory and Practice (4th ed.). Oxfordshire, England: Scion Publishing Ltd. * Kumar, G. & Rudbeck, L. (2009). Dako Education Guide Special Stains and H & E (2nd ed.). Carpinteria, CA: Dako North America. * Kumar, G. & Rudbeck, L. (2009) Dako Immunohistochemical Staining Methods (5th ed.) Carpinteria, CA: Dako North America. * Harmening, D.M. (2012). Laboratory Management: Principles and Processes (3rd ed.). St. Petersburg: D.H. Pub. & Consulting, Inc. Please advise. MSgt Ian R Bernard, HT(ASCP), MSHA-UAB Anatomic Pathology Lab Manager USAF- Active Duty 210-687-7540 Cell ibernard@uab.edu ian.bernard@comcast.net From joelleweaver <@t> hotmail.com Mon Mar 3 07:31:31 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Mar 3 07:31:34 2014 Subject: [Histonet] Best Study material for HTL In-Reply-To: References: Message-ID: just my "two cents" Basics; Carson- A great instructional text, good for teaching, if you need the basics, this is good if you get the white, 3rd edition. The purple-magenta edition is a little superficial in my opinion for concentrated study. Would add- Sheehan & Hrapchak, a little outdated but more in depth theory, "classic" histotechnology. Luna would also suffice for this purpose. Staining theory;Kiernan, J. (2008). Histological and Histochemical Methods: GOOD, but I believe expensive.Kumar, G. & Rudbeck, L. (2009). Dako Education Guide Special Stains and H & E (2nd ed.). Good for introductory on special stains, not both Carson and this, maybe one of them if you need basics first.Kumar, G. & Rudbeck, L. (2009) Dako Immunohistochemical Staining Methods (5th ed.) -GOOD IHC review.Harmening, D.M. (2012). Laboratory Management: Principles and Processes (3rd ed.). St. Petersburg: D.H. Pub. & Consulting, Inc. Don't have this one, may be good if you do not have a management or business background? Note, books are like music, and each person will be drawn to particular ways of presenting the same content, they all have some good points. I am just responding with what I would choose if I were to have to study all over again. Best of luck. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: ibernard@uab.edu > To: histonet@lists.utsouthwestern.edu > Date: Sun, 2 Mar 2014 21:53:15 +0000 > Subject: [Histonet] Best Study material for HTL > > Fellow Histonetters. I will sit for the HTL exam over next three to four months. Per the ASCP suggested list, the following study materials were suggested as study for the HTL. Any thoughts on narrowing the list of study materials or are all materials indicted necessary? > > > > > * Carson, F. & Hladik, C. (2009). Histotechnology: A Self-Instructional Text (3rd ed.). Chicago: ASCP Press. > > * Kiernan, J. (2008). Histological and Histochemical Methods: Theory and Practice (4th ed.). Oxfordshire, England: Scion Publishing Ltd. > > * Kumar, G. & Rudbeck, L. (2009). Dako Education Guide Special Stains and H & E (2nd ed.). Carpinteria, CA: Dako North America. > > * Kumar, G. & Rudbeck, L. (2009) Dako Immunohistochemical Staining Methods (5th ed.) Carpinteria, CA: Dako North America. > > * Harmening, D.M. (2012). Laboratory Management: Principles and Processes (3rd ed.). St. Petersburg: D.H. Pub. & Consulting, Inc. > > > > Please advise. > > MSgt Ian R Bernard, HT(ASCP), MSHA-UAB > Anatomic Pathology Lab Manager > USAF- Active Duty > 210-687-7540 Cell > ibernard@uab.edu > ian.bernard@comcast.net > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone <@t> leavittmgt.com Mon Mar 3 07:32:52 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon Mar 3 07:32:41 2014 Subject: [Histonet] PT/PER DIEM Histotech DELRAY BCH, FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CB08A@vm-email.leavittmgt.com> Hi Histonetters! We are still interviewing for a part time tech here in our very busy east Delray FLORIDA Dermatology Lab (upwards of 80k cases/year). This is a permanent part time position with full time growth potential. PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES!!! Email your resume to lengimann@leavittmgt.com if interested. MUST BE LICENSED!!!! *part time position AND per diem available with FLEXIBLE TIMES (24 hour facility) *MUST be licensed as a FL histotechnologist or technician *MUST have at least 2 years experience (dermatology preferred) *very proficient in embedding and microtomy *experience in grossing and immunohistochemistry (BOND) a plus *must be self motivated and a team player (UNPROFESSIONALISM WILL NOT BE TOLERATED) *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm 561.819.0857 ext 100 561.819.6517 fax ksimeone@leavittmgt.com www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From johnronan <@t> custompathlabsolutions.com Mon Mar 3 10:31:52 2014 From: johnronan <@t> custompathlabsolutions.com (Custompathlabsolutions) Date: Mon Mar 3 10:32:01 2014 Subject: [Histonet] For Sale - Leica bond Max Message-ID: Hi all - We have a Leica Bond Max for sale. It was purchased in 2011 and was underutilized until September 2013 when it was taken out of service. Our IHC volume does not warrant automation. It is in excellent condition and has been maintained by Leica. Please contact me for photos and info. John Ronan Histology Manager NY Associates in Gastroenterology Johnronan@custompathlabsolutions.com 732 406-3384 Sent from my iPhone From tony.auge <@t> gmail.com Mon Mar 3 10:51:22 2014 From: tony.auge <@t> gmail.com (Tony Auge) Date: Mon Mar 3 10:51:32 2014 Subject: [Histonet] Best Study material for HTL In-Reply-To: References: Message-ID: This study guide is pretty handy: http://www.amazon.com/Practice-Questions-Histotechnology-Examinations-Registry/dp/089189473X/ref=sr_1_1?ie=UTF8&qid=1393865047&sr=8-1&keywords=histotechnology+bor I used that and Carson to study for my HTL. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com From BZIMMERM <@t> gru.edu Mon Mar 3 15:24:26 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Mon Mar 3 15:24:39 2014 Subject: [Histonet] HISTOPALOOZA GSH APRIL 25 - 27, 2014 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF81B816@EX-MLB-03.ad.georgiahealth.edu> The 41st annual GSH symposium will be here before you know it. I can't believe it's already March. Isn't that how I know I'm getting old? I've started talking about how time flies, my knee hurts, my shoulder hurts, but at least I'm not talking about my bowel habits, yet. LOL Anyway... another fantastic speaker to mention here today. On Saturday, April 26th, Lamar Jones will be giving a workshop at 8:30 titled "Breast Cancer: From Surgery to Microscope". In the workshop Lamar will discuss the updated ASCO/CAP breast guidelines, proper fixation, processing, H&E staining and IHC expression. Case studies will also be discussed. If you haven't had the pleasure of taking one of Lamar's workshops, make a point to take this one. If you are awaiting funds, please go ahead and register now ! This will ensure your spot. I'm looking forward to seeing all of you at The Lodge and Spa at Callaway Gardens. http://www.histosearch.com/gsh/ Billie Zimmerman GSH Secretary From JMacDonald <@t> mtsac.edu Mon Mar 3 15:53:31 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Mar 3 16:03:56 2014 Subject: [Histonet] Best Study material for HTL In-Reply-To: References: Message-ID: There is a newer addition of this student guide available through the NSH or ASCP. If you are a member there is a reduced price. http://www.ascp.org/Store/Books/BOC-Study-Guide-Histotechnology.html#StoreList From: Tony Auge To: joelle weaver Cc: "histonet@lists.utsouthwestern.edu" Date: 03/03/2014 08:54 AM Subject: Re: [Histonet] Best Study material for HTL Sent by: histonet-bounces@lists.utsouthwestern.edu This study guide is pretty handy: http://www.amazon.com/Practice-Questions-Histotechnology-Examinations-Registry/dp/089189473X/ref=sr_1_1?ie=UTF8&qid=1393865047&sr=8-1&keywords=histotechnology+bor I used that and Carson to study for my HTL. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Mar 4 06:51:28 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Mar 4 06:51:33 2014 Subject: [Histonet] 88342 - again Message-ID: <5A2BD13465E061429D6455C8D6B40E391681B31093@IBMB7Exchange.digestivespecialists.com> Good morning, Has anyone seen or know anything yet as to how insurances are paying for multiple 88342's on a case for non-Medicare patients? It seems as though no one yet is sure what is going on. Thanks, Linda From jamie <@t> prometheushealthcare.com Tue Mar 4 07:10:57 2014 From: jamie <@t> prometheushealthcare.com (Jamie Buxton) Date: Tue Mar 4 07:13:24 2014 Subject: [Histonet] Histology Opportunity in New Jersey Message-ID: <07fb01cf37ab$314aba70$93e02f50$@prometheushealthcare.com> I am currently recruiting for a several month temp assignment with a laboratory located in New Jersey. For immediate consideration and additional information, please feel free to contact me at Jamie@prometheushealthcare.com Jamie Buxton Recruiter Prometheus Healthcare Direct Line (407) 780-2582 Office (301) 693-9057 Fax (301) 368-2478 Jamie@prometheushealthcare.com www.prometheushealthcare.com From Joyce.Weems <@t> emoryhealthcare.org Tue Mar 4 08:14:54 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Mar 4 08:15:03 2014 Subject: [Histonet] RE: 88342 - again In-Reply-To: <5A2BD13465E061429D6455C8D6B40E391681B31093@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E391681B31093@IBMB7Exchange.digestivespecialists.com> Message-ID: Our pathologists have learned that on the professional side some private carriers are billing for only one even if there are multiples on the same specimen. (United specifically) They have also learned that Medicare will not pay for multiples unless they are on separate lines. e.g. if 10 IHC on one specimen and you bill G0461 x 1 G0462 x 9 Medicare will reimburse for 2 immunos. You must have G0462 on 9 separate lines. I have not been able to find out how the hospital is being reimbursed yet. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, March 04, 2014 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 88342 - again Good morning, Has anyone seen or know anything yet as to how insurances are paying for multiple 88342's on a case for non-Medicare patients? It seems as though no one yet is sure what is going on. Thanks, Linda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From BergerR1 <@t> email.chop.edu Tue Mar 4 08:49:57 2014 From: BergerR1 <@t> email.chop.edu (Berger, Rebecca) Date: Tue Mar 4 08:50:12 2014 Subject: [Histonet] Masson's Tri-chome on Frozen Section Message-ID: <5A590EB108038B4FA01F8CDB39C6541B0131CE3E@EXCMBXPW7.chop.edu> Hi there, Does anyone has a protocol for Masson's Trichrome on frozen sections? Thanks! Becky From rmweber113 <@t> comcast.net Tue Mar 4 09:46:26 2014 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Tue Mar 4 09:46:42 2014 Subject: [Histonet] 88305 Medicare change Message-ID: <2045771740.165302.1393947986238.JavaMail.root@sz0187a.westchester.pa.mail.comcast.net> Hi,? Has anyone out there heard of a change for the 88305 code for Medicare.? In the case of a prostate biopsy we would normally charge 88305 x 12.? Now I am hearing that we must charge 88305 for the first 9 and G0416 for the additional 4.? Has anyone heard of the same? Thank you, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC From BZIMMERM <@t> gru.edu Tue Mar 4 10:36:46 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Mar 4 10:36:53 2014 Subject: [Histonet] HISTOPALOOZA APRIL 25 - 27, 2014 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF81DCE3@EX-MLB-03.ad.georgiahealth.edu> Today's featured speaker for the GSH Histopalooza is Elizabeth (Ely) Klar. Ely's workshop will be held on Sunday, April 27th from 8:30 - 12:00. The title is " Human Tissue: Histological Identification of the Major Types". This workshop is very popular and extremely informative. The histological features of the different types and categories of tissues will be described to facilitate identification of human tissue and organs. It will be well worth your time to attend this workshop. On another note, please renew your FREE membership to GSH online and take a look at our newsletter, the Microtime. If you have issues reserving your room please contact Taylor Reames at the Lodge. The number is 706-489-3359. Tomorrow, Wanda and I will be having a photo op with Governor Nathan Deal under the gold dome. We'll be there for the state proclamation for Histotechnology Professionals Day March 10th, touching lives, one slide at a time. We plan to visit the salon first and our VP Mike Bourgeois will get his comb-over ready for the photo while I'm hoping for a great photoshop job on my middle age gut. LOL Billie Zimmerman GSH secretary From l.kammili <@t> yahoo.com Tue Mar 4 11:23:00 2014 From: l.kammili <@t> yahoo.com (Lakshmi Kammili) Date: Tue Mar 4 11:23:06 2014 Subject: [Histonet] Image J analysis on Sirius red stain Message-ID: <1393953780.17132.YahooMailNeo@web121003.mail.ne1.yahoo.com> Hi all,? I am Lakshmi, research associate from GWU pathology core lab. ?I am doing a project on sirius red stain analysis using image J software. I am having tuff time in setting thresholds and clearing the background. if any one of you have experience with this topic please email me l.kammili@yahoo.com. Thank you in advance.? Lakshmi Kammili.? From rajanbhatt <@t> gmail.com Tue Mar 4 12:33:51 2014 From: rajanbhatt <@t> gmail.com (Gmail-rajanbhatt) Date: Tue Mar 4 12:33:58 2014 Subject: [Histonet] HT Certified Histotech for Phoenix Dermatology Office Needed- Please Apply Message-ID: <2E4555DE-918B-4E27-B425-20EA1720A42F@gmail.com> Hi?We are a dermatology practice in the Phoenix/Arcadia area and are in need of either a full time or part time HT Certified Histotech who knows how to run a dermpath lab and maintain CLIA. If you can work as a Mohs Tech as well, this is an added bonus. Salary is commensurate with experience. To Apply, please send email and resume to: rajanbhatt@gmail.com Thank you, Rajan Bhatt, MD From shultz11 <@t> cox.net Tue Mar 4 13:03:52 2014 From: shultz11 <@t> cox.net (shultz11) Date: Tue Mar 4 13:04:03 2014 Subject: [Histonet] Job Message-ID: <2nrkhhy6sinecnf7pxvgulsr.1393959832730@email.android.com> Histology technician job at Louisiana State University. Go to lsu.edu. Sent from my Verizon Wireless 4G LTE Smartphone From relia1 <@t> earthlink.net Tue Mar 4 13:14:55 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Mar 4 13:15:07 2014 Subject: [Histonet] RELIA Histology Careers Weekly Update. Happy Mardi Gras! Message-ID: <0bf301cf37de$06fd1370$14f73a50$@earthlink.net> Hi Histonetters!! Happy Mardi Gras!!!! Are you doing anything special to celebrate Mardi Gras? New Orleans has had a special place in my heart for a long time. My husband proposed to me there many years ago and we try to get there at least once a year. Since we won't be in NOLA for Mardi Gras this year we found a Mardi Gras block party to go to here in town. So. Laissez Les Bon Temps Rouler! That means let the good times roll!! I hope you have a chance to add some fun and frivolity to your day!! Instead of asking YOU to "THROW ME SOMETHING MISTER" Please take a look at my current job openings! All of these are permanent full time openings. I am proud to say that all of my clients offer competitive pay, great benefits and great people to work with. Relocation is negotiable. Histology Management Atlanta, GA Columbus, OH Seattle, WA - A RELIA EXCLUSIVE!!! Histology Technician/Technologist Boston, MA Atlanta, GA Columbus, OH Seattle, WA- A RELIA EXCLUSIVE!! Louisville, KY If you or anyone you know is interested in hearing more about any of the opportunities or exploring a job search in another area please contact me. You can reach me at relia1@earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. I will be happy to help, everything is kept in strictest of confidence and if I place someone you refer to me you will earn a referral fee. Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2014 is Histotechnology Professionals Day. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA From Glenn.Hauck <@t> albertahealthservices.ca Tue Mar 4 13:22:01 2014 From: Glenn.Hauck <@t> albertahealthservices.ca (Glenn Hauck) Date: Tue Mar 4 13:22:09 2014 Subject: [Histonet] Lymph node fixative Message-ID: <38FA8FCA474F834CB9B90590D7C72390015AFC8D33D0@EXMBX4C.crha.bewell.ca> I am trying to find out if lymph node fixatives should be used to help in identifying lymph nodes in axillary breast tissue. I was under the impression that the use may affect immunohistochemistry including ER/PR and HER2 results. Does anyone have experience out there or documentation that I could read through. Thanks Glenn Hauck Charge Tecnologist Pathology QueenElizabeth II Hospital Grande Prairie, AB T8V 2E8 780-538-7429 Work Main 780-538-7184 Work Office glenn.hauck@albertahealthservices.ca ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From roxannes40 <@t> gmail.com Tue Mar 4 13:30:48 2014 From: roxannes40 <@t> gmail.com (Roxanne) Date: Tue Mar 4 13:30:54 2014 Subject: [Histonet] Medicare 88305 In-Reply-To: <531614c8.44603c0a.60e4.ffffe5b5SMTPIN_ADDED_MISSING@mx.google.com> References: <531614c8.44603c0a.60e4.ffffe5b5SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: The code is g0416 for 10-20 prostate specimens. It is not both 88305 and g0416. If you have a prostate biopsy case with 6 specimens, the cpt codes would be 88305x6. If you have a prostate biopsy case with 16 specimens, the code would be g0416. Sent from my iPhone > On Mar 4, 2014, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. HISTOPALOOZA GSH APRIL 25 - 27, 2014 (Zimmerman, Billie) > 2. Re: Best Study material for HTL (Jennifer MacDonald) > 3. 88342 - again (Blazek, Linda) > 4. Histology Opportunity in New Jersey (Jamie Buxton) > 5. RE: 88342 - again (Weems, Joyce K.) > 6. Masson's Tri-chome on Frozen Section (Berger, Rebecca) > 7. 88305 Medicare change (rmweber113@comcast.net) > 8. HISTOPALOOZA APRIL 25 - 27, 2014 (Zimmerman, Billie) > 9. Image J analysis on Sirius red stain (Lakshmi Kammili) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 3 Mar 2014 21:24:26 +0000 > From: "Zimmerman, Billie" > Subject: [Histonet] HISTOPALOOZA GSH APRIL 25 - 27, 2014 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <7B3DEB32E69C034EACB479059C5DE3FF81B816@EX-MLB-03.ad.georgiahealth.edu> > > Content-Type: text/plain; charset="us-ascii" > > The 41st annual GSH symposium will be here before you know it. I can't believe it's already March. Isn't that how I know I'm getting old? I've started talking about how time flies, my knee hurts, my shoulder hurts, but at least I'm not talking about my bowel habits, yet. LOL > Anyway... another fantastic speaker to mention here today. On Saturday, April 26th, Lamar Jones will be giving a workshop at 8:30 titled "Breast Cancer: From Surgery to Microscope". > In the workshop Lamar will discuss the updated ASCO/CAP breast guidelines, proper fixation, processing, H&E staining and IHC expression. Case studies will also be discussed. > If you haven't had the pleasure of taking one of Lamar's workshops, make a point to take this one. If you are awaiting funds, please go ahead and register now ! This will ensure your spot. > I'm looking forward to seeing all of you at The Lodge and Spa at Callaway Gardens. > http://www.histosearch.com/gsh/ > > Billie Zimmerman > GSH Secretary > > > ------------------------------ > > Message: 2 > Date: Mon, 3 Mar 2014 13:53:31 -0800 > From: Jennifer MacDonald > Subject: Re: [Histonet] Best Study material for HTL > To: Tony Auge > Cc: "histonet@lists.utsouthwestern.edu" > , > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > There is a newer addition of this student guide available through the NSH > or ASCP. If you are a member there is a reduced price. > > http://www.ascp.org/Store/Books/BOC-Study-Guide-Histotechnology.html#StoreList > > > > > From: Tony Auge > To: joelle weaver > Cc: "histonet@lists.utsouthwestern.edu" > > Date: 03/03/2014 08:54 AM > Subject: Re: [Histonet] Best Study material for HTL > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > This study guide is pretty handy: > > http://www.amazon.com/Practice-Questions-Histotechnology-Examinations-Registry/dp/089189473X/ref=sr_1_1?ie=UTF8&qid=1393865047&sr=8-1&keywords=histotechnology+bor > > > I used that and Carson to study for my HTL. > > > -- > > Tony Auge HTL (ASCP) QIHC > Histology Supervisor - Chandler Pathology Services > Cell: (651) 373-4768 > Email: tony.auge@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Tue, 4 Mar 2014 07:51:28 -0500 > From: "Blazek, Linda" > Subject: [Histonet] 88342 - again > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <5A2BD13465E061429D6455C8D6B40E391681B31093@IBMB7Exchange.digestivespecialists.com> > > Content-Type: text/plain; charset="us-ascii" > > Good morning, > > Has anyone seen or know anything yet as to how insurances are paying for multiple 88342's on a case for non-Medicare patients? It seems as though no one yet is sure what is going on. > Thanks, > Linda > > > > ------------------------------ > > Message: 4 > Date: Tue, 4 Mar 2014 08:10:57 -0500 > From: "Jamie Buxton" > Subject: [Histonet] Histology Opportunity in New Jersey > To: > Message-ID: <07fb01cf37ab$314aba70$93e02f50$@prometheushealthcare.com> > Content-Type: text/plain; charset="us-ascii" > > I am currently recruiting for a several month temp assignment with a > laboratory located in New Jersey. For immediate consideration and additional > information, please feel free to contact me at > Jamie@prometheushealthcare.com > > > > Jamie Buxton > Recruiter > > Prometheus Healthcare > > Direct Line (407) 780-2582 > > Office (301) 693-9057 > > Fax (301) 368-2478 > > Jamie@prometheushealthcare.com > > www.prometheushealthcare.com > > > > > > ------------------------------ > > Message: 5 > Date: Tue, 4 Mar 2014 14:14:54 +0000 > From: "Weems, Joyce K." > Subject: [Histonet] RE: 88342 - again > To: "'Blazek, Linda'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Our pathologists have learned that on the professional side some private carriers are billing for only one even if there are multiples on the same specimen. (United specifically) > > They have also learned that Medicare will not pay for multiples unless they are on separate lines. > e.g. if 10 IHC on one specimen and you bill > G0461 x 1 > G0462 x 9 > Medicare will reimburse for 2 immunos. > You must have G0462 on 9 separate lines. > > I have not been able to find out how the hospital is being reimbursed yet. > > > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda > Sent: Tuesday, March 04, 2014 7:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] 88342 - again > > Good morning, > > Has anyone seen or know anything yet as to how insurances are paying for multiple 88342's on a case for non-Medicare patients? It seems as though no one yet is sure what is going on. > Thanks, > Linda > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > > > ------------------------------ > > Message: 6 > Date: Tue, 4 Mar 2014 14:49:57 +0000 > From: "Berger, Rebecca" > Subject: [Histonet] Masson's Tri-chome on Frozen Section > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <5A590EB108038B4FA01F8CDB39C6541B0131CE3E@EXCMBXPW7.chop.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Hi there, > Does anyone has a protocol for Masson's Trichrome on frozen sections? > Thanks! > Becky > > > ------------------------------ > > Message: 7 > Date: Tue, 4 Mar 2014 15:46:26 +0000 (UTC) > From: rmweber113@comcast.net > Subject: [Histonet] 88305 Medicare change > To: histonet > Message-ID: > <2045771740.165302.1393947986238.JavaMail.root@sz0187a.westchester.pa.mail.comcast.net> > > Content-Type: text/plain; charset=utf-8 > > > > Hi,? Has anyone out there heard of a change for the 88305 code for Medicare.? In the case of a prostate biopsy we would normally charge 88305 x 12.? Now I am hearing that we must charge 88305 for the first 9 and G0416 for the additional 4.? Has anyone heard of the same? > > > > Thank you, > > > Marilynn Weber H.T.(ASCP)QIHC > Coastal Pathology Consulting Services LLC > > > ------------------------------ > > Message: 8 > Date: Tue, 4 Mar 2014 16:36:46 +0000 > From: "Zimmerman, Billie" > Subject: [Histonet] HISTOPALOOZA APRIL 25 - 27, 2014 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <7B3DEB32E69C034EACB479059C5DE3FF81DCE3@EX-MLB-03.ad.georgiahealth.edu> > > Content-Type: text/plain; charset="us-ascii" > > Today's featured speaker for the GSH Histopalooza is Elizabeth (Ely) Klar. Ely's workshop will be held on Sunday, April 27th from 8:30 - 12:00. The title is " Human Tissue: Histological Identification of the Major Types". This workshop is very popular and extremely informative. The histological features of the different types and categories of tissues will be described to facilitate identification of human tissue and organs. It will be well worth your time to attend this workshop. > On another note, please renew your FREE membership to GSH online and take a look at our newsletter, the Microtime. If you have issues reserving your room please contact Taylor Reames at the Lodge. The number is 706-489-3359. Tomorrow, Wanda and I will be having a photo op with Governor Nathan Deal under the gold dome. We'll be there for the state proclamation for Histotechnology Professionals Day March 10th, touching lives, one slide at a time. We plan to visit the salon first and our VP Mike Bourgeois will get his comb-over ready for the photo while I'm hoping for a great photoshop job on my middle age gut. LOL > > Billie Zimmerman > GSH secretary > > > ------------------------------ > > Message: 9 > Date: Tue, 4 Mar 2014 09:23:00 -0800 (PST) > From: Lakshmi Kammili > Subject: [Histonet] Image J analysis on Sirius red stain > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1393953780.17132.YahooMailNeo@web121003.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi all, > > I am Lakshmi, research associate from GWU pathology core lab. I am doing a project on sirius red stain analysis using image J software. I am having tuff time in setting thresholds and clearing the background. if any one of you have experience with this topic please email me l.kammili@yahoo.com. > > Thank you in advance. > > Lakshmi Kammili. > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 124, Issue 4 > **************************************** From thisisann <@t> aol.com Tue Mar 4 17:05:12 2014 From: thisisann <@t> aol.com (Ann Specian) Date: Tue Mar 4 17:05:19 2014 Subject: [Histonet] SOP for submitting slides for HQIP Message-ID: <8D1061554B29B05-4BCC-64A3@webmail-d220.sysops.aol.com> I have to write an sop for submission of slides for HQIP. Does anyone have one they can share? Thanks, Ann From CThornton <@t> dahlchase.com Wed Mar 5 09:26:28 2014 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Wed Mar 5 09:26:33 2014 Subject: [Histonet] Aspergillus Message-ID: Does anyone have any FFPE Aspergillus tissue they can share? Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From relia1 <@t> earthlink.net Wed Mar 5 09:31:24 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Mar 5 09:31:40 2014 Subject: [Histonet] RELIA Hot JOB ALERT ------ BRAND NEW OPPORTUNITY - CHARLOTTE NC A RELIA EXCLUSIVE!!!! Message-ID: <00e301cf3887$f7d18e80$e774ab80$@earthlink.net> Hi Histonetters!! I hope you are all having a great day!! One of my very BEST clients just called me to let me know that they are hiring and I am excited!! Why am I excited and why do I say they are one of my best clients? Because no one EVER leaves... the only reason they have openings is because of growth! Also because they only work with ME. Yes this is a RELIA EXCLUSIVE and has been for over 5 years!! Here are the details that I am able to share online: This is a full time permanent M-F 7a-430p position in a private path lab. You would be doing grossing, routine histology and IHC. ASCP certification and CLIA qualification for grossing are required. My client offers a very competitive compensation package. Their first priority would be to hire a local candidate but everything is negotiable for the right person. If you are interested please contact me right away. You can reach me by phone toll free at 866-607-3542 or on my cell at 407-353-5070 or by email at relia1@earthlink.net If you refer someone else and I place them you will earn a referral fee. Make it a great day!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2014 is Histotechnology Professionals Day. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 <@t> earthlink.net Wed Mar 5 09:32:23 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Mar 5 09:32:38 2014 Subject: [Histonet] RELIA Hot JOB ALERT ------ BRAND NEW OPPORTUNITY - CHARLOTTE NC A RELIA EXCLUSIVE!!!! Message-ID: <00ea01cf3888$1b2e2370$518a6a50$@earthlink.net> Hi Histonetters!! I hope you are all having a great day!! One of my very BEST clients just called me to let me know that they are hiring and I am excited!! Why am I excited and why do I say they are one of my best clients? Because no one EVER leaves. the only reason they have openings is because of growth! Also because they only work with ME. Yes this is a RELIA EXCLUSIVE and has been for over 5 years!! Here are the details that I am able to share online: This is a full time permanent M-F 7a-430p position in a private path lab. You would be doing grossing, routine histology and IHC. ASCP certification and CLIA qualification for grossing are required. My client offers a very competitive compensation package. Their first priority would be to hire a local candidate but everything is negotiable for the right person. If you are interested please contact me right away. You can reach me by phone toll free at 866-607-3542 or on my cell at 407-353-5070 or by email at relia1@earthlink.net If you refer someone else and I place them you will earn a referral fee. Make it a great day!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2014 is Histotechnology Professionals Day. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rob <@t> foliobio.com Wed Mar 5 09:33:45 2014 From: rob <@t> foliobio.com (Rob Day) Date: Wed Mar 5 09:34:01 2014 Subject: [Histonet] Aspergillus In-Reply-To: References: Message-ID: I'm pretty sure that the Oakley lab in Kansas would be happy to help you. http://www2.ku.edu/~mb/cgi-bin/viewprofile.shtml?id=27 Regards, Rob Day. On Mar 5, 2014, at 10:26 AM, Clare Thornton wrote: > Does anyone have any FFPE Aspergillus tissue they can share? > > Clare J. Thornton, HTL(ASCP)QIHC > Lead Immunohistochemistry Technologist > Dahl-Chase Diagnostic Services > 417 State Street, Suite 540 > Bangor, ME 04401 > cthornton@dahlchase.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 skype: invasifspecies http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. Visit my blogs here. From Kim.Kolman <@t> va.gov Wed Mar 5 10:49:52 2014 From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.) Date: Wed Mar 5 10:50:29 2014 Subject: [Histonet] cryostat design Message-ID: <9C32F30B6662D74A8419DDDB7E66656A02517AAE@VHAV15MSGA1.v15.med.va.gov> Hello all; Is anyone aware of a cryostat design in which the microtome cabinet would sit at bench level? In other words, it would function like any regular bench microtome but be housed in a cold cabinet like a cryostat? This would enable the operator to work in a desk position instead of uncomfortably being hunched over a cryostat cabinet. Thanks in advance, Kim Kimberly D. Kolman, HT (ASCP) VA Eastern Kansas Health Care System 4101 S 4th St Trfwy Leavenworth KS 66048 913-682-2000x 52537 Fax: 913-758-4193 From abright <@t> brightinstruments.com Wed Mar 5 11:04:58 2014 From: abright <@t> brightinstruments.com (Alan Bright) Date: Wed Mar 5 11:05:04 2014 Subject: [Histonet] cryostat design In-Reply-To: <9C32F30B6662D74A8419DDDB7E66656A02517AAE@VHAV15MSGA1.v15.med.va.gov> References: <9C32F30B6662D74A8419DDDB7E66656A02517AAE@VHAV15MSGA1.v15.med.va.gov> Message-ID: Dear Kim, Yes the Starlet cryostat made by Bright Instrument Company, it is in a small bench level cabinet as there is no other way of achieving good results on the refrigeration. Apart from freezing microtones which were highly inefficient on retrieving good quality thin sections. Hope this assists you. Alan Bright BIC......UK Sent from my iPhone > On 5 Mar 2014, at 16:55, "Kolman, Kimberly D." wrote: > > Hello all; > > Is anyone aware of a cryostat design in which the microtome cabinet > would sit at bench level? In other words, it would function like any > regular bench microtome but be housed in a cold cabinet like a cryostat? > This would enable the operator to work in a desk position instead of > uncomfortably being hunched over a cryostat cabinet. > > > > Thanks in advance, > > Kim > > > > > > Kimberly D. Kolman, HT (ASCP) > > VA Eastern Kansas Health Care System > > 4101 S 4th St Trfwy > > Leavenworth KS 66048 > > 913-682-2000x 52537 > > Fax: 913-758-4193 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > From Vickroy.Jim <@t> mhsil.com Wed Mar 5 12:55:24 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Mar 5 12:55:29 2014 Subject: [Histonet] HPV by ISH Message-ID: How are others accomplishing proficiency testing of HPV ISH? We send our HPV ISH testing to be done by GenPath and then our pathologists interpret the slides that are sent back. Any ideas? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From CROUTHIER <@t> PARTNERS.ORG Wed Mar 5 13:13:39 2014 From: CROUTHIER <@t> PARTNERS.ORG (Routhier, Caitlin Ann) Date: Wed Mar 5 13:14:02 2014 Subject: [Histonet] Cryosectioning Issue Message-ID: I am having trouble sectioning a skeletal muscle specimen we received in our lab for enzyme histochemistry. It's an extremely small piece of tissue (0.5 x 0.1 x 0.05 cm) and I can't seem to get a real section when cutting on the cryostat. The roll plate is not helping at all and any tissue I do get seems to end up crumpled up on the slide (they seem pretty much uninterruptable to me). Any help/suggestions/hints on what I might be doing wrong would so so so appreciated!!!!!!! Thanks!!! :) Caitlin The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Mark.Elliott <@t> hli.ubc.ca Wed Mar 5 15:00:04 2014 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Wed Mar 5 15:01:09 2014 Subject: [Histonet] Icam-1 antibody for mouse tissue. Message-ID: <53171FD4020000D60006D2C9@mail.hli.ubc.ca> Can anyone recommend an antibody to ICam-1 that is not made in a mouse. I need to stain mouse tissues for ICam-1 and would like very much to avoid having to do mouse-on-mouse. Thanks for all suggestions Mark From CDavis <@t> che-east.org Thu Mar 6 08:00:30 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu Mar 6 08:01:13 2014 Subject: [Histonet] CPT codes & Medicare Message-ID: <08861B9CF6C7774E874635A4818AE37B088180C80C@CHEXCMS01.one.ads.che.org> If anyone needs info. on the new billing/charging CPT codes, this was shared with me so I thought I could make it available to you. http://www.cap.org/apps/portlets/contentViewer/show.do?printFriendly=true&contentReference=statline%2Findex.html# Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From kalschev <@t> svm.vetmed.wisc.edu Thu Mar 6 09:24:09 2014 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Thu Mar 6 09:24:14 2014 Subject: [Histonet] bone protocol/Trevor J Wait Message-ID: <703A683FAF544F48A56D054A24EDF6B7@vetmed.wisc.edu> Trevor, Feel free to contact me. I have worked with decalcified bone (undecalcified, as well) for a number of year. Vicki Vicki Kalscheur Department of Surgical Sciences School of Veterinary Medicine University of Wisconsin 2015 Linden Drive Madison, WI 53706-1102 Phone: 608-262-8534 FAX: 608-263-7930 kalschev@svm.vetmed.wisc.edu www.vetmed.wisc.edu/research/orthop From Haley.Huggins <@t> DignityHealth.org Thu Mar 6 09:41:22 2014 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Thu Mar 6 09:41:29 2014 Subject: [Histonet] Special Stains with Zinc formalin fixed tissue Message-ID: <4F36EC93A5737D4F8A2974E8FB8E2606173EEF39BE@PHX-MSG-007-N2.chw.edu> Hello fellow histonetters, I am new to working with zinc formalin and am having issues with staining special stains. Does anyone have any advice to troubleshoot this problem? I am particularly having trouble with AB/PAS and PAS stains. Any assistance is appreciated. Thank you. Haley Haley Huggins, HT(ASCP)cm Pathology/Histology Manager Marian Medical Center 1400 East Church St Santa Maria, CA 93454 805-739-3170 (path lab) 805-739-3153 (office) 303-652-7453 (cell) 805-332-8697 (rightfax) From Rebecca.Riesen <@t> hma.com Thu Mar 6 10:47:39 2014 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Thu Mar 6 10:47:08 2014 Subject: [Histonet] Stain on posters Message-ID: What is the stain on the HT Prof Day posters? Rebecca S. Riesen, HT, ASCP Histology Supervisor PRHS Naples, FL 239-348-4327 rebecca.riesen@hma.com From TanyaAbbott <@t> catholichealth.net Thu Mar 6 10:47:08 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Thu Mar 6 10:47:12 2014 Subject: [Histonet] Antibody validation Message-ID: <852F7D2C14FB464D80E182B15DB138AF3064E0AE@CHIEX005.CHI.catholichealth.net> I am wondering what process labs are doing to validate new antibodies per CAP guidelines? Thanks! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From algranth <@t> email.arizona.edu Thu Mar 6 12:46:44 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Mar 6 12:47:51 2014 Subject: [Histonet] Cost per test Message-ID: <0B9F6E21-75B4-444A-B310-E0E69B4B5868@email.arizona.edu> Rene, I know that you have discussed this on histonet several times and you have written a paper - can you send me a copy of your paper? We are having to do some accounting here to justify our charges and I think your paper would help. Andrea Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From lblazek <@t> digestivespecialists.com Thu Mar 6 12:53:07 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 6 12:53:13 2014 Subject: [Histonet] Cost per test In-Reply-To: <0B9F6E21-75B4-444A-B310-E0E69B4B5868@email.arizona.edu> References: <0B9F6E21-75B4-444A-B310-E0E69B4B5868@email.arizona.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E391681B31999@IBMB7Exchange.digestivespecialists.com> You might want to get ahold of Tim Morken also. He has a great spreadsheet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, March 06, 2014 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost per test Rene, I know that you have discussed this on histonet several times and you have written a paper - can you send me a copy of your paper? We are having to do some accounting here to justify our charges and I think your paper would help. Andrea Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Thu Mar 6 13:27:11 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Mar 6 13:39:30 2014 Subject: [Histonet] RE: cryostat design In-Reply-To: <9C32F30B6662D74A8419DDDB7E66656A02517AAE@VHAV15MSGA1.v15.med.va.gov> References: <9C32F30B6662D74A8419DDDB7E66656A02517AAE@VHAV15MSGA1.v15.med.va.gov> Message-ID: Leica has a more ergonomically correct cryostat, which sits lower and your feet are on the ground. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kolman, Kimberly D. Sent: Wednesday, March 05, 2014 8:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat design Hello all; Is anyone aware of a cryostat design in which the microtome cabinet would sit at bench level? In other words, it would function like any regular bench microtome but be housed in a cold cabinet like a cryostat? This would enable the operator to work in a desk position instead of uncomfortably being hunched over a cryostat cabinet. Thanks in advance, Kim Kimberly D. Kolman, HT (ASCP) VA Eastern Kansas Health Care System 4101 S 4th St Trfwy Leavenworth KS 66048 913-682-2000x 52537 Fax: 913-758-4193 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Thu Mar 6 14:26:19 2014 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Thu Mar 6 14:26:23 2014 Subject: [Histonet] Cailtin - muscle on cryostat Message-ID: <5A9A7B5679594E17AC2B1F71C3E46F13@vetmed.wisc.edu> Hi Cailtin, If time allows, look up the cryojane system. I am not certain who manufactures' it anymore, however it can be a good investment for difficult sections. We use it for tendon & cruciate. It fits into the cryostat and is easy to use. You likely will not need it. There are many talented folks cutting muscle and sometimes is a simple hint or phone conversation that remedies the problem. Best regards, Vicki From Sandy.Cope-Yokoyama <@t> childrens.com Thu Mar 6 16:24:28 2014 From: Sandy.Cope-Yokoyama <@t> childrens.com (Sandy Cope-yokoyama) Date: Thu Mar 6 16:24:50 2014 Subject: [Histonet] FW: fixation and processing/reprocessing protocol for pig skin. Message-ID: <6F81318C8F5F984D972778B918A7D76915F73ABF@CMCPBEXMAIL12.Childrens.med> Forwarded for a histonet member: ______________________________ Dear Histonetters, I have a scientist who is studying the pig skin. This tissue is so hard that almost impossible to section. I?ll be appreciated if you can send me a fixation and processing/reprocessing protocol for pig skin. My best regards, Naira DesignTemplate Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From abright <@t> brightinstruments.com Fri Mar 7 02:58:58 2014 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Mar 7 02:59:09 2014 Subject: [Histonet] Cryosectioning Issue In-Reply-To: References: Message-ID: <6EDBAAB5-19AA-48AF-ACDE-0FFEBB2C7A2D@brightinstruments.com> What cryostat type are you having this issue with. Alan Bright BIC Uk Sent from my iPhone > On 5 Mar 2014, at 19:18, "Routhier, Caitlin Ann" wrote: > > I am having trouble sectioning a skeletal muscle specimen we received in our lab for enzyme histochemistry. It's an extremely small piece of tissue (0.5 x 0.1 x 0.05 cm) and I can't seem to get a real section when cutting on the cryostat. The roll plate is not helping at all and any tissue I do get seems to end up crumpled up on the slide (they seem pretty much uninterruptable to me). Any help/suggestions/hints on what I might be doing wrong would so so so appreciated!!!!!!! > > Thanks!!! :) > Caitlin > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > From TGoins <@t> mt.gov Fri Mar 7 11:03:18 2014 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Fri Mar 7 11:03:27 2014 Subject: [Histonet] Mailing Freebie Message-ID: OK - I may be missing something, but what is that "blue adhesive" pocket that was sent out with histology professionals' day literature? Just curious . . . . . Happy Friday. Tresa From LSebree <@t> uwhealth.org Fri Mar 7 11:21:55 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Fri Mar 7 11:22:02 2014 Subject: [Histonet] RE: Mailing Freebie In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF09CA41@UWHC-MBX12.uwhis.hosp.wisc.edu> I was told "they're in the mail". Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, March 07, 2014 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mailing Freebie OK - I may be missing something, but what is that "blue adhesive" pocket that was sent out with histology professionals' day literature? Just curious . . . . . Happy Friday. Tresa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Mar 7 11:31:30 2014 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Fri Mar 7 11:31:28 2014 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gTWFpbGluZyBGcmVlYmll?= Message-ID: I thought earlier this week they sent out an explanatory email saying it was a phone holder? Perhaps I dreamt this, it has been a week. Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Goins, Tresa" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Mailing Freebie Date: Fri, Mar 7, 2014 12:03 pm OK - I may be missing something, but what is that "blue adhesive" pocket that was sent out with histology professionals' day literature? Just curious . . . . . Happy Friday. Tresa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Mar 7 11:34:36 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Mar 7 11:34:43 2014 Subject: [Histonet] Mailing Freebie In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391681B31C7C@IBMB7Exchange.digestivespecialists.com> You're supposed to stick it on your phone and put your money in it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelleweaver@hotmail.com Sent: Friday, March 07, 2014 12:32 PM To: Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mailing Freebie I thought earlier this week they sent out an explanatory email saying it was a phone holder? Perhaps I dreamt this, it has been a week. Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Goins, Tresa" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Mailing Freebie Date: Fri, Mar 7, 2014 12:03 pm OK - I may be missing something, but what is that "blue adhesive" pocket that was sent out with histology professionals' day literature? Just curious . . . . . Happy Friday. Tresa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Mar 7 11:47:32 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Mar 7 11:47:46 2014 Subject: [Histonet] Recall: NSH Mystery Member Gift Message-ID: Pam Barker would like to recall the message, "NSH Mystery Member Gift". From relia1 <@t> earthlink.net Fri Mar 7 11:47:59 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Mar 7 11:48:16 2014 Subject: [Histonet] FW: NSH Mystery Member Gift In-Reply-To: <20448576.1394214385879.JavaMail.root@wamui-june.atl.sa.earthlink.net> References: <20448576.1394214385879.JavaMail.root@wamui-june.atl.sa.earthlink.net> Message-ID: TGIF! Scroll down and the mystery will be revealed! Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2014 is Histotechnology Professionals Day. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From: Pam Barker [mailto:relia1@earthlink.net] Sent: Friday, March 07, 2014 12:46 PM To: relia1@earthlink.net Subject: Fw: NSH Mystery Member Gift -----Forwarded Message----- From: National Society for Histotechnology Sent: Mar 5, 2014 3:13 PM To: relia1@earthlink.net Subject: NSH Mystery Member Gift ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Still trying to figure out what that blue pocket thing is that NSH sent? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ It's a Phone Wallet! ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ The mystery gift is a little like the field of Histotechnology, a mystery to many, but the name in itself can make people ask the question. So as a reminder to get out there and promote your profession on March 10th for Histotechnology Professionals Day [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxjIb7Ld0fOneivJ74FYnwBArHgagDmgAk2WHP-yT8_B8PM6FRsIkWLKlqdq4S6IpkZym-uKXTucbL_nVJxs-R_zobYNbl5br-anYGZpmhx8bCsoNUat80OhgtDQqdikyzg==&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==], the mystery gifts are Phone Wallets. Stick them to the back of your cell phone to hold your credit cards, ID, badge, or a little cash to get something to drink at the gym. Just a little something to so you have the bare essentials at your fingertips without having to carry bags, purses and wallets everyplace you go. Did you end up using the phone wallet for something else? Maybe a pocket on the side of your microtome? Post a picture to our Facebook Page [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxjIb7Ld0fOneivJ74FYnwBArHgagDmgAk2WHP-yT8_B8PM6FRsIkWLKlqdq4S6IpkZym-uKXTucbL_nVJxs-R_zobYNbl5br-anYGZpmhx8bCsoNUat80OhgtDQqdikyzg==&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==] using #histoday, we want to see how creative you can be :) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Important Information: Keep an eye out for an email on Monday for Scavenger Hunt & Free Webinar information!! ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Please remember to order your items to help celebrate your special day! ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ HPD ORDER FORM [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxrnDY4lBar0_XgvWpEFRrIt_Rb3wuyO2swbfxDCrNlS38RPWLGfn3n9yJnAj2ywLYtlSAzgfpnQW_joEchNz8shPwf_djJXv3n4kBLIErfHiF9bpfXYx_wZ1Kla_NPjeJptW0YvvCu_Y05Mawwg7Of0FJUQlQD4vso5D5l3gRk3BH1MekGXYzCA=&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==] Like us on Facebook [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxrnDY4lBar0_cVYP0EpSgkH2UbfVRARG-R8v4Xnjg_UGzWCriulkgI7zkzQUGHzZugOCix1Fo8sR9ZQM750vbcK8HLOWSA1w_smVFOoyMyDH1fUycXCVgKYlff5mpB5DU2-gae26BpJSShPFcsCozLg=&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==] Follow us on Twitter [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxh62zB1QiSGKhIZMkr1wgaVoNzqjD9xzIHZl-RAthQVhaq657fJf-c5TJBXAXO82sVJhYdYAdHIXyrhlvzcbHViTeqGxw6bZI_T_wxxHydzdX_NXW6xBwqKs4o7NOtuu-A==&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==] #Histoday ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This year's Histotechnology Professionals Day (HPD) Logo has the dog face stain created by Cheryl Willetts from Lakewood, WA. She was the winner of last year's HPD Staintastic contest. Thanks Cheryl for an awesome piece of art. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Forward this email http://ui.constantcontact.com/sa/fwtf.jsp?llr=skbtxxcab &m=1102455604562&ea=relia1@earthlink.net&a=1116731623806 This email was sent to relia1@earthlink.net by histo@nsh.org. Update Profile/Email Address http://visitor.constantcontact.com/do?p=oo &m=0010Ze_QsIJhTVwsqDCXlL6ew%3D%3D&ch=3b927f70-3325-11e3-824c-d4ae527536ce&ca=cb737373-5f88-4c83-b766-fddfcdd470da Instant removal with SafeUnsubscribe(TM) http://visitor.constantcontact.com/do?p=un &m=0010Ze_QsIJhTVwsqDCXlL6ew%3D%3D&ch=3b927f70-3325-11e3-824c-d4ae527536ce&ca=cb737373-5f88-4c83-b766-fddfcdd470da Privacy Policy: http://ui.constantcontact.com/roving/CCPrivacyPolicy.jsp Online Marketing by Constant Contact(R) www.constantcontact.com National Society for Histotechnology | 8850 Stanford Blvd | Suite 2900 | Columbia | MD | 21045 Thank you! Pam M. Barker President RELIA 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: 407-657-2027 Fax: 407-678-2788 Cell: 407-353-5070 e-mail: relia1@earthlink.net Toll Free: 866-60-RELIA (866-607-3542) From jfray80 <@t> hotmail.com Fri Mar 7 12:29:34 2014 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Fri Mar 7 12:29:39 2014 Subject: [Histonet] Sakura Express 120 Message-ID: I am looking for anyone who is using the x-120 Sakura Express 120 processor . could you please reply to my email jfray80@hotmail.com I would also like to talk with someone personally. From CROUTHIER <@t> PARTNERS.ORG Fri Mar 7 12:36:33 2014 From: CROUTHIER <@t> PARTNERS.ORG (Routhier, Caitlin Ann) Date: Fri Mar 7 12:37:11 2014 Subject: [Histonet] NSH'S Mystery Gift In-Reply-To: References: Message-ID: Yup it's a holder for your phone (I saw the email last night). Supposedly you can stick your credit/debit cards, license, cash etc in there instead of carrying a purse (or wallet for all those boy histologists out there) :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, March 07, 2014 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 124, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Cost per test (Grantham, Andrea L - (algranth)) 2. RE: Cost per test (Blazek, Linda) 3. RE: cryostat design (Bea DeBrosse-Serra) 4. Cailtin - muscle on cryostat (Vicki Kalscheur) 5. FW: fixation and processing/reprocessing protocol for pig skin. (Sandy Cope-yokoyama) 6. Re: Cryosectioning Issue (Alan Bright) 7. Mailing Freebie (Goins, Tresa) 8. RE: Mailing Freebie (Sebree Linda A) 9. Re: Mailing Freebie (joelleweaver@hotmail.com) 10. RE: Mailing Freebie (Blazek, Linda) 11. Recall: NSH Mystery Member Gift (Pam Barker) 12. FW: NSH Mystery Member Gift (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Thu, 6 Mar 2014 18:46:44 +0000 From: "Grantham, Andrea L - (algranth)" Subject: [Histonet] Cost per test To: "histonet@lists.utsouthwestern.edu" Message-ID: <0B9F6E21-75B4-444A-B310-E0E69B4B5868@email.arizona.edu> Content-Type: text/plain; charset="us-ascii" Rene, I know that you have discussed this on histonet several times and you have written a paper - can you send me a copy of your paper? We are having to do some accounting here to justify our charges and I think your paper would help. Andrea Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ------------------------------ Message: 2 Date: Thu, 6 Mar 2014 13:53:07 -0500 From: "Blazek, Linda" Subject: RE: [Histonet] Cost per test To: "Grantham, Andrea L - (algranth)" , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E391681B31999@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" You might want to get ahold of Tim Morken also. He has a great spreadsheet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, March 06, 2014 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost per test Rene, I know that you have discussed this on histonet several times and you have written a paper - can you send me a copy of your paper? We are having to do some accounting here to justify our charges and I think your paper would help. Andrea Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 6 Mar 2014 19:27:11 +0000 From: Bea DeBrosse-Serra Subject: [Histonet] RE: cryostat design To: "'Kolman, Kimberly D.'" , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Leica has a more ergonomically correct cryostat, which sits lower and your feet are on the ground. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kolman, Kimberly D. Sent: Wednesday, March 05, 2014 8:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cryostat design Hello all; Is anyone aware of a cryostat design in which the microtome cabinet would sit at bench level? In other words, it would function like any regular bench microtome but be housed in a cold cabinet like a cryostat? This would enable the operator to work in a desk position instead of uncomfortably being hunched over a cryostat cabinet. Thanks in advance, Kim Kimberly D. Kolman, HT (ASCP) VA Eastern Kansas Health Care System 4101 S 4th St Trfwy Leavenworth KS 66048 913-682-2000x 52537 Fax: 913-758-4193 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 6 Mar 2014 14:26:19 -0600 From: "Vicki Kalscheur" Subject: [Histonet] Cailtin - muscle on cryostat To: Message-ID: <5A9A7B5679594E17AC2B1F71C3E46F13@vetmed.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Hi Cailtin, If time allows, look up the cryojane system. I am not certain who manufactures' it anymore, however it can be a good investment for difficult sections. We use it for tendon & cruciate. It fits into the cryostat and is easy to use. You likely will not need it. There are many talented folks cutting muscle and sometimes is a simple hint or phone conversation that remedies the problem. Best regards, Vicki ------------------------------ Message: 5 Date: Thu, 6 Mar 2014 22:24:28 +0000 From: Sandy Cope-yokoyama Subject: [Histonet] FW: fixation and processing/reprocessing protocol for pig skin. To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <6F81318C8F5F984D972778B918A7D76915F73ABF@CMCPBEXMAIL12.Childrens.med> Content-Type: text/plain; charset="utf-8" Forwarded for a histonet member: ______________________________ Dear Histonetters, I have a scientist who is studying the pig skin. This tissue is so hard that almost impossible to section. I???ll be appreciated if you can send me a fixation and processing/reprocessing protocol for pig skin. My best regards, Naira DesignTemplate Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ------------------------------ Message: 6 Date: Fri, 7 Mar 2014 08:58:58 +0000 From: "Alan Bright" Subject: Re: [Histonet] Cryosectioning Issue To: "Routhier, Caitlin Ann" Cc: histonet@lists.utsouthwestern.edu Message-ID: <6EDBAAB5-19AA-48AF-ACDE-0FFEBB2C7A2D@brightinstruments.com> Content-Type: text/plain; charset="us-ascii" What cryostat type are you having this issue with. Alan Bright BIC Uk Sent from my iPhone > On 5 Mar 2014, at 19:18, "Routhier, Caitlin Ann" wrote: > > I am having trouble sectioning a skeletal muscle specimen we received in our lab for enzyme histochemistry. It's an extremely small piece of tissue (0.5 x 0.1 x 0.05 cm) and I can't seem to get a real section when cutting on the cryostat. The roll plate is not helping at all and any tissue I do get seems to end up crumpled up on the slide (they seem pretty much uninterruptable to me). Any help/suggestions/hints on what I might be doing wrong would so so so appreciated!!!!!!! > > Thanks!!! :) > Caitlin > > > The information in this e-mail is intended only for the person to whom > it is addressed. If you believe this e-mail was sent to you in error > and the e-mail contains patient information, please contact the > Partners Compliance HelpLine at http://www.partners.org/complianceline > . If the e-mail was sent to you in error but does not contain patient > information, please contact the sender and properly dispose of the e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > ------------------------------ Message: 7 Date: Fri, 7 Mar 2014 17:03:18 +0000 From: "Goins, Tresa" Subject: [Histonet] Mailing Freebie To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" OK - I may be missing something, but what is that "blue adhesive" pocket that was sent out with histology professionals' day literature? Just curious . . . . . Happy Friday. Tresa ------------------------------ Message: 8 Date: Fri, 7 Mar 2014 17:21:55 +0000 From: Sebree Linda A Subject: [Histonet] RE: Mailing Freebie To: "'Goins, Tresa'" , "histonet@lists.utsouthwestern.edu" Message-ID: <77DD817201982748BC67D7960F2F76AF09CA41@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" I was told "they're in the mail". Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, March 07, 2014 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mailing Freebie OK - I may be missing something, but what is that "blue adhesive" pocket that was sent out with histology professionals' day literature? Just curious . . . . . Happy Friday. Tresa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 7 Mar 2014 12:31:30 -0500 From: joelleweaver@hotmail.com Subject: Re: [Histonet] Mailing Freebie To: " Goins, Tresa " , " histonet@lists.utsouthwestern.edu " Message-ID: Content-Type: text/plain; charset="utf-8" I thought earlier this week they sent out an explanatory email saying it was a phone holder? Perhaps I dreamt this, it has been a week. Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Goins, Tresa" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Mailing Freebie Date: Fri, Mar 7, 2014 12:03 pm OK - I may be missing something, but what is that "blue adhesive" pocket that was sent out with histology professionals' day literature? Just curious . . . . . Happy Friday. Tresa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 7 Mar 2014 12:34:36 -0500 From: "Blazek, Linda" Subject: RE: [Histonet] Mailing Freebie To: "joelleweaver@hotmail.com" , "Goins, Tresa" , "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E391681B31C7C@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="utf-8" You're supposed to stick it on your phone and put your money in it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelleweaver@hotmail.com Sent: Friday, March 07, 2014 12:32 PM To: Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mailing Freebie I thought earlier this week they sent out an explanatory email saying it was a phone holder? Perhaps I dreamt this, it has been a week. Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Goins, Tresa" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Mailing Freebie Date: Fri, Mar 7, 2014 12:03 pm OK - I may be missing something, but what is that "blue adhesive" pocket that was sent out with histology professionals' day literature? Just curious . . . . . Happy Friday. Tresa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 7 Mar 2014 12:47:32 -0500 From: "Pam Barker" Subject: [Histonet] Recall: NSH Mystery Member Gift To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Pam Barker would like to recall the message, "NSH Mystery Member Gift". ------------------------------ Message: 12 Date: Fri, 7 Mar 2014 12:47:59 -0500 From: "Pam Barker" Subject: [Histonet] FW: NSH Mystery Member Gift To: Message-ID: Content-Type: text/plain; charset="utf-8" TGIF! Scroll down and the mystery will be revealed! Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2014 is Histotechnology Professionals Day. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From: Pam Barker [mailto:relia1@earthlink.net] Sent: Friday, March 07, 2014 12:46 PM To: relia1@earthlink.net Subject: Fw: NSH Mystery Member Gift -----Forwarded Message----- From: National Society for Histotechnology Sent: Mar 5, 2014 3:13 PM To: relia1@earthlink.net Subject: NSH Mystery Member Gift ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Still trying to figure out what that blue pocket thing is that NSH sent? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ It's a Phone Wallet! ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ The mystery gift is a little like the field of Histotechnology, a mystery to many, but the name in itself can make people ask the question. So as a reminder to get out there and promote your profession on March 10th for Histotechnology Professionals Day [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxjIb7Ld0fOneivJ74FYnwBArHgagDmgAk2WHP-yT8_B8PM6FRsIkWLKlqdq4S6IpkZym-uKXTucbL_nVJxs-R_zobYNbl5br-anYGZpmhx8bCsoNUat80OhgtDQqdikyzg==&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==], the mystery gifts are Phone Wallets. Stick them to the back of your cell phone to hold your credit cards, ID, badge, or a little cash to get something to drink at the gym. Just a little something to so you have the bare essentials at your fingertips without having to carry bags, purses and wallets everyplace you go. Did you end up using the phone wallet for something else? Maybe a pocket on the side of your microtome? Post a picture to our Facebook Page [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxjIb7Ld0fOneivJ74FYnwBArHgagDmgAk2WHP-yT8_B8PM6FRsIkWLKlqdq4S6IpkZym-uKXTucbL_nVJxs-R_zobYNbl5br-anYGZpmhx8bCsoNUat80OhgtDQqdikyzg==&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==] using #histoday, we want to see how creative you can be :) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Important Information: Keep an eye out for an email on Monday for Scavenger Hunt & Free Webinar information!! ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Please remember to order your items to help celebrate your special day! ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ HPD ORDER FORM [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxrnDY4lBar0_XgvWpEFRrIt_Rb3wuyO2swbfxDCrNlS38RPWLGfn3n9yJnAj2ywLYtlSAzgfpnQW_joEchNz8shPwf_djJXv3n4kBLIErfHiF9bpfXYx_wZ1Kla_NPjeJptW0YvvCu_Y05Mawwg7Of0FJUQlQD4vso5D5l3gRk3BH1MekGXYzCA=&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==] Like us on Facebook [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxrnDY4lBar0_cVYP0EpSgkH2UbfVRARG-R8v4Xnjg_UGzWCriulkgI7zkzQUGHzZugOCix1Fo8sR9ZQM750vbcK8HLOWSA1w_smVFOoyMyDH1fUycXCVgKYlff5mpB5DU2-gae26BpJSShPFcsCozLg=&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==] Follow us on Twitter [http://r20.rs6.net/tn.jsp?f=001wUVYiDruAZ3hoo0BIBDIsYoGJnqmMX9pz-Y7l3Ekd_50-9jYqZQGxh62zB1QiSGKhIZMkr1wgaVoNzqjD9xzIHZl-RAthQVhaq657fJf-c5TJBXAXO82sVJhYdYAdHIXyrhlvzcbHViTeqGxw6bZI_T_wxxHydzdX_NXW6xBwqKs4o7NOtuu-A==&c=4fk54EJBU-P_tl-Oirl8sWGh4-JLc91Rpz9o9UxqFnGqvHqKBI2pXQ==&ch=y5qD7RJPs_r1S5l5ntbbMcTD_Hd8d87_TYGPPYjUKFfBP1aQWHsAwQ==] #Histoday ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This year's Histotechnology Professionals Day (HPD) Logo has the dog face stain created by Cheryl Willetts from Lakewood, WA. She was the winner of last year's HPD Staintastic contest. Thanks Cheryl for an awesome piece of art. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Forward this email http://ui.constantcontact.com/sa/fwtf.jsp?llr=skbtxxcab &m=1102455604562&ea=relia1@earthlink.net&a=1116731623806 This email was sent to relia1@earthlink.net by histo@nsh.org. Update Profile/Email Address http://visitor.constantcontact.com/do?p=oo &m=0010Ze_QsIJhTVwsqDCXlL6ew%3D%3D&ch=3b927f70-3325-11e3-824c-d4ae527536ce&ca=cb737373-5f88-4c83-b766-fddfcdd470da Instant removal with SafeUnsubscribe(TM) http://visitor.constantcontact.com/do?p=un &m=0010Ze_QsIJhTVwsqDCXlL6ew%3D%3D&ch=3b927f70-3325-11e3-824c-d4ae527536ce&ca=cb737373-5f88-4c83-b766-fddfcdd470da Privacy Policy: http://ui.constantcontact.com/roving/CCPrivacyPolicy.jsp Online Marketing by Constant Contact(R) www.constantcontact.com National Society for Histotechnology | 8850 Stanford Blvd | Suite 2900 | Columbia | MD | 21045 Thank you! Pam M. Barker President RELIA 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: 407-657-2027 Fax: 407-678-2788 Cell: 407-353-5070 e-mail: relia1@earthlink.net Toll Free: 866-60-RELIA (866-607-3542) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 124, Issue 7 **************************************** From joelleweaver <@t> hotmail.com Fri Mar 7 12:47:29 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Mar 7 12:47:33 2014 Subject: [Histonet] Mailing Freebie In-Reply-To: <5A2BD13465E061429D6455C8D6B40E391681B31C7C@IBMB7Exchange.digestivespecialists.com> References: , <5A2BD13465E061429D6455C8D6B40E391681B31C7C@IBMB7Exchange.digestivespecialists.com> Message-ID: Ahh... thanks. They could have included an example item of money :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lblazek@digestivespecialists.com > To: joelleweaver@hotmail.com; TGoins@mt.gov; histonet@lists.utsouthwestern.edu > Date: Fri, 7 Mar 2014 12:34:36 -0500 > Subject: RE: [Histonet] Mailing Freebie > > You're supposed to stick it on your phone and put your money in it. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelleweaver@hotmail.com > Sent: Friday, March 07, 2014 12:32 PM > To: Goins, Tresa; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Mailing Freebie > > I thought earlier this week they sent out an explanatory email saying it was a phone holder? Perhaps I dreamt this, it has been a week. > > Sent from my Verizon Wireless 4G LTE Smartphone > > ----- Reply message ----- > From: "Goins, Tresa" > To: "histonet@lists.utsouthwestern.edu" > Subject: [Histonet] Mailing Freebie > Date: Fri, Mar 7, 2014 12:03 pm > > > > > OK - I may be missing something, but what is that "blue adhesive" pocket that was sent out with histology professionals' day literature? > Just curious . . . . . > > Happy Friday. > > Tresa > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brandihisto <@t> hotmail.com Fri Mar 7 15:02:30 2014 From: brandihisto <@t> hotmail.com (Brandi) Date: Fri Mar 7 15:02:35 2014 Subject: [Histonet] eCC CAP cancer protocols Message-ID: Is anyone using this? I couldn't easily see what the cost is. My facility uses Meditech...always looking for ways to keep up with the ever changing protocols! Thanks, Brandi Farris From WaitT <@t> livemail.uthscsa.edu Fri Mar 7 15:22:00 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Fri Mar 7 15:22:07 2014 Subject: [Histonet] Low vs. High Profile Blades Message-ID: Hey does anyone know the difference between a High Profile Blade vs. a Low Profile Blade when using for sectioning of Paraffin embedded tissues specimens? Which one would ya'll prefer for decalcified bone? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry From joelleweaver <@t> hotmail.com Sat Mar 8 09:04:14 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Mar 8 09:04:23 2014 Subject: [Histonet] Low vs. High Profile Blades In-Reply-To: References: Message-ID: I think this is largely personal preference, but manufacturers have marketed specific blades as being designed for certain purposes. I like low profile for paraffin sectioning of biopsies and well processed specimens, but that is mostly because I learned to section with low profile in histology school initially doing high volume with many different tissues, and I just learned to adjust my technique accordingly using that type of blade. I do prefer high profile in the cryostat however, but probably also because of my experiences with cryosectioning different tissues using high profile. I am not sure if there is much published science on this ( I looked once when researching paraffin composition and crystallization against blade resistance, and couldn't find much). I personally feel that for very hard or dense tissues there may be some advantage in stability and durability for high profile, since they are thicker ( more clamping surface) and perhaps a "tougher" less refined edge, that might be able to stay sharper longer with the greater resistance. But I have used both low & high with human bone ( decalcified or not) with roughly equal success. I am not sure if the difference would be more substantial and noticeable with animal tissues. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: WaitT@livemail.uthscsa.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 7 Mar 2014 21:22:00 +0000 > Subject: [Histonet] Low vs. High Profile Blades > > Hey does anyone know the difference between a High Profile Blade vs. a Low Profile Blade when using for sectioning of Paraffin embedded tissues specimens? Which one would ya'll prefer for decalcified bone? > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio > Class of 2017 MD Candidate > Abilene Christian University Class of 2013 Graduate > B.S. Biochemistry > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Mar 8 09:10:24 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Mar 8 09:10:29 2014 Subject: [Histonet] eCC CAP cancer protocols In-Reply-To: References: Message-ID: Are you talking about the biomarker cancer-synoptic reporting templates as pdf files from the webpage, or the electronic templates for purchase? I am getting ready to build this into LIS next week, and it would be helpful if you can elaborate. If I am missing anything that I can load prebuilt following CAP protocols this will save tons of time for me. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: brandihisto@hotmail.com > Date: Fri, 7 Mar 2014 15:02:30 -0600 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] eCC CAP cancer protocols > > Is anyone using this? I couldn't easily see what the cost is. My facility uses Meditech...always looking for ways to keep up with the ever changing protocols! > > Thanks, > Brandi Farris > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Mon Mar 10 03:20:32 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Mon Mar 10 03:20:40 2014 Subject: [Histonet] RE: Low vs. High Profile Blades In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FB3F5491E@FWDCWPMSGCMS09.hca.corpad.net> We use the low profile for everything...Accu-Edge. They are superior. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Friday, March 07, 2014 4:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Low vs. High Profile Blades Hey does anyone know the difference between a High Profile Blade vs. a Low Profile Blade when using for sectioning of Paraffin embedded tissues specimens? Which one would ya'll prefer for decalcified bone? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Mon Mar 10 08:33:41 2014 From: akemiat3377 <@t> yahoo.com (Eileen Akemi Allison-Tacha) Date: Mon Mar 10 08:33:44 2014 Subject: [Histonet] Our Day! Message-ID: <6A680366-DC8C-49F8-B04C-4E76EC08E771@yahoo.com> Happy Histotechnology Professionals Day to All my Colleagues! Did you know it is also Dezna Sheehan's Birthday too? I am honored to have known her. What an incredible mentor. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aallison@montereygi.com Tele: (831) 375-3577 X117 From jfray80 <@t> hotmail.com Mon Mar 10 10:04:03 2014 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Mon Mar 10 10:04:07 2014 Subject: [Histonet] FW: Sakura Express 120 In-Reply-To: References: Message-ID: Repost X120 Processor From: jfray80@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: Sakura Express 120 Date: Fri, 7 Mar 2014 12:29:34 -0600 I am looking for anyone who is using the x-120 Sakura Express 120 processor . could you please reply to my email jfray80@hotmail.com I would also like to talk with someone personally. I am not in the market to purchase , From tkngflght <@t> yahoo.com Mon Mar 10 15:29:12 2014 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Mon Mar 10 15:29:20 2014 Subject: [Histonet] Happy histology day! Message-ID: <8DA6070E-B835-4C96-90EB-8F688014447B@yahoo.com> Almost missed it! xoxox Cheryl From Bauer.Karen <@t> mayo.edu Tue Mar 11 10:24:40 2014 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Mar 11 10:24:48 2014 Subject: [Histonet] Pathology Protocols Message-ID: <6e55ab$8gr6v9@ironport10.mayo.edu> Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org From joewalker <@t> rrmc.org Tue Mar 11 11:21:03 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Mar 11 11:21:17 2014 Subject: [Histonet] RE: Pathology Protocols In-Reply-To: <6e55ab$8gr6v9@ironport10.mayo.edu> References: <6e55ab$8gr6v9@ironport10.mayo.edu> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1822F323@RRMBX03.rrmc.local> Hi Karen, We utilize Cerner Millennium at my institution. We are 90% paper free. Fortunately for us, the vast majority of our surg path orders come to us as an electronic order, either placed by the provider on the floor or through an integrated office EMR. All op notes, office notes, radiology, etc are also available electronically to the pathologist at the time of the gross dictation. The gross dictation is transcribed into the surg path report prior to the pathologist getting their slides the next day. The pathologist uses either a bar code to scan the slide/accession number to bring up the surg path report with the transcribed gross dictation. The pathologist can again access all of the op notes, etc at the time of the final report's dictation. Also, all clinical test results are available within the application the pathologist uses to view the reports. Our transcriptionist manages the movement of cases to and from the pathologists' queue for their verification. The 10% of paper requisitions that we receive are ordered by our administrative staff where all of the written information on the paper is transferred to the electronic order. The paper reqs are then scanned into our system. Once in our system, the process for the paper reports is the same as the above. The exception is that we generally don't have office notes when we receive orders on paper. A couple of our pathologist do utilize a dual monitor set up that allows them to have the surg path report and the clinical info like radiology on a different monitor for their comparison. It didn't take a lot of convincing to get rid of the paper and has helped improve the accuracy of the orders and diagnostic information. There were definitely adjustments to everyone's workflows but we have been operating this way for 2 years now with very few problems. If you have the capability, I'd highly encourage removing the paper process, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, March 11, 2014 11:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology Protocols Importance: Low Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From Bauer.Karen <@t> mayo.edu Tue Mar 11 11:24:24 2014 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Mar 11 11:25:32 2014 Subject: [Histonet] Pathology Protocols In-Reply-To: <002401cf3d42$418c18b0$c4a44a10$@caplab.org> References: <6e55ab$8gr6v9@ironport10.mayo.edu> <002401cf3d42$418c18b0$c4a44a10$@caplab.org> Message-ID: <6e55ab$8gs31h@ironport10.mayo.edu> Hi Douglas, Yes... For the most part, they are on board with this... So long we can get the information that they want/need on the screen/monitor(s). We just need to figure out how to make that happen. Figured I'd ask out in Histoland to see if we can learn from someone who has already made/created that process. Thanks for the reply! :) Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: Douglas Porter [mailto:doug.porter@caplab.org] Sent: Tuesday, March 11, 2014 10:55 AM To: Bauer, Karen L.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pathology Protocols The key to your plan is having pathologists that are willing and/or able to use the computer to do something normally done by someone else. Once you cross this barrier, the rest is logistics. Good Luck!! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, March 11, 2014 11:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology Protocols Importance: Low Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4335 / Virus Database: 3722/7177 - Release Date: 03/10/14 From trathborne <@t> somerset-healthcare.com Tue Mar 11 11:34:23 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 11 11:35:02 2014 Subject: [Histonet] RE: Pathology Protocols In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC1822F323@RRMBX03.rrmc.local> References: <6e55ab$8gr6v9@ironport10.mayo.edu> <3C2378778400AD448ADA6FD6BDB7CCCC1822F323@RRMBX03.rrmc.local> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95BEA43@SMCMAIL01.somerset-healthcare.com> Joe, I'm just curious about handwritten notes. We also have Cerner Millennium, but when I tried a few years ago to find out if there would be an impact in any of the areas, the OR reminded me that some of the surgeons like to draw diagrams for margins. The pathologists also document on the paper requisition when and who was called with frozen results. Although this is a small percentage of the specimens we receive, it stalled the project. How do you get around this? Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, March 11, 2014 12:21 PM To: Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Pathology Protocols Hi Karen, We utilize Cerner Millennium at my institution. We are 90% paper free. Fortunately for us, the vast majority of our surg path orders come to us as an electronic order, either placed by the provider on the floor or through an integrated office EMR. All op notes, office notes, radiology, etc are also available electronically to the pathologist at the time of the gross dictation. The gross dictation is transcribed into the surg path report prior to the pathologist getting their slides the next day. The pathologist uses either a bar code to scan the slide/accession number to bring up the surg path report with the transcribed gross dictation. The pathologist can again access all of the op notes, etc at the time of the final report's dictation. Also, all clinical test results are available within the application the pathologist uses to view the reports. Our transcriptionist manages the movement of cases to and from the pathologists' queue for their verification. The 10% of paper requisitions that we receive are ordered by our administrative staff where all of the written information on the paper is transferred to the electronic order. The paper reqs are then scanned into our system. Once in our system, the process for the paper reports is the same as the above. The exception is that we generally don't have office notes when we receive orders on paper. A couple of our pathologist do utilize a dual monitor set up that allows them to have the surg path report and the clinical info like radiology on a different monitor for their comparison. It didn't take a lot of convincing to get rid of the paper and has helped improve the accuracy of the orders and diagnostic information. There were definitely adjustments to everyone's workflows but we have been operating this way for 2 years now with very few problems. If you have the capability, I'd highly encourage removing the paper process, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, March 11, 2014 11:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology Protocols Importance: Low Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Mar 11 11:50:50 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Mar 11 11:51:07 2014 Subject: [Histonet] RE: Pathology Protocols In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95BEA43@SMCMAIL01.somerset-healthcare.com> References: <6e55ab$8gr6v9@ironport10.mayo.edu> <3C2378778400AD448ADA6FD6BDB7CCCC1822F323@RRMBX03.rrmc.local> <3AD061FE740D464FAC7BF6B5CFB75707A95BEA43@SMCMAIL01.somerset-healthcare.com> Message-ID: <761E2B5697F795489C8710BCC72141FF3675D53B@ex07.net.ucsf.edu> Toni wrote: " I'm just curious about handwritten notes. We also have Cerner Millennium, but when I tried a few years ago to find out if there would be an impact in any of the areas, the OR reminded me that some of the surgeons like to draw diagrams for margins. The pathologists also document on the paper requisition when and who was called with frozen results. Although this is a small percentage of the specimens we receive, it stalled the project. How do you get around this?" We have Copath Plus (Cerner) and recently installed PicsPLus to capture all paperwork. Everything will be scanned into the case file for access later. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, March 11, 2014 9:34 AM To: 'Joe W. Walker, Jr.'; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Pathology Protocols Joe, I'm just curious about handwritten notes. We also have Cerner Millennium, but when I tried a few years ago to find out if there would be an impact in any of the areas, the OR reminded me that some of the surgeons like to draw diagrams for margins. The pathologists also document on the paper requisition when and who was called with frozen results. Although this is a small percentage of the specimens we receive, it stalled the project. How do you get around this? Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, March 11, 2014 12:21 PM To: Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Pathology Protocols Hi Karen, We utilize Cerner Millennium at my institution. We are 90% paper free. Fortunately for us, the vast majority of our surg path orders come to us as an electronic order, either placed by the provider on the floor or through an integrated office EMR. All op notes, office notes, radiology, etc are also available electronically to the pathologist at the time of the gross dictation. The gross dictation is transcribed into the surg path report prior to the pathologist getting their slides the next day. The pathologist uses either a bar code to scan the slide/accession number to bring up the surg path report with the transcribed gross dictation. The pathologist can again access all of the op notes, etc at the time of the final report's dictation. Also, all clinical test results are available within the application the pathologist uses to view the reports. Our transcriptionist manages the movement of cases to and from the pathologists' queue for their verification. The 10% of paper requisitions that we receive are ordered by our administrative staff where all of the written information on the paper is transferred to the electronic order. The paper reqs are then scanned into our system. Once in our system, the process for the paper reports is the same as the above. The exception is that we generally don't have office notes when we receive orders on paper. A couple of our pathologist do utilize a dual monitor set up that allows them to have the surg path report and the clinical info like radiology on a different monitor for their comparison. It didn't take a lot of convincing to get rid of the paper and has helped improve the accuracy of the orders and diagnostic information. There were definitely adjustments to everyone's workflows but we have been operating this way for 2 years now with very few problems. If you have the capability, I'd highly encourage removing the paper process, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, March 11, 2014 11:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology Protocols Importance: Low Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Tue Mar 11 11:56:21 2014 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Mar 11 11:56:31 2014 Subject: [Histonet] Pathology Protocols In-Reply-To: <6e55ab$8gr6v9@ironport10.mayo.edu> References: <6e55ab$8gr6v9@ironport10.mayo.edu> Message-ID: <02d701cf3d4a$d51c4e30$7f54ea90$@pathview.com> Karen, it all depends on your LIS's functionality and whether you have a separate tracking system or not. I'm an LIS vendor, but I'll describe how a 5 hospital client works: 1. Electronic and paper orders are accessioned. 2. All paperwork is barcoded as it is accessioned. 3. Paperwork is scanned and attached to the case 'automatically'. 4. At grossing, the barcoded specimen is scanned and barcoded cassettes print. Simultaneously, the patient history and requisition appears on the screen for confirmation. 5. At transcription, this same history, current case and requisition appears and the transcriptionist does a double check of information as well as type. 6. This client does not use tracking at the processor or embedding by choice, but they do at 'cutting' so blocks are scanned and the associated slides are printed. 7. skipping some steps for brevity. 8. At pathologist workcenter, the pathologist scans the barcoded slides and the case with history displays. Any previous quality alerts are highlighted. The pathologist can enter additional alerts, enter their diagnosis, etc. So, yes, they are completely 'paperless', use Dragon for dictation of diagnoses, not gross at the moment, and do this for cytology as well as surgical cases. In your situation, you have a middleware tracking system so it brings up some questions. Do you want grossing to see the paperwork? Sometimes there are diagrams that are relevant. If you do, then your tracking system is where you'll want to scan the documents into. On the other hand if you want the documents to be available to the transcriptionist or the pathologist, you may want to scan into your LIS. It's all about the workflow of your laboratory, right..... Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, March 11, 2014 8:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology Protocols Importance: Low Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joewalker <@t> rrmc.org Tue Mar 11 11:57:58 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Mar 11 11:58:06 2014 Subject: [Histonet] RE: Pathology Protocols In-Reply-To: <761E2B5697F795489C8710BCC72141FF3675D53B@ex07.net.ucsf.edu> References: <6e55ab$8gr6v9@ironport10.mayo.edu> <3C2378778400AD448ADA6FD6BDB7CCCC1822F323@RRMBX03.rrmc.local> <3AD061FE740D464FAC7BF6B5CFB75707A95BEA43@SMCMAIL01.somerset-healthcare.com> <761E2B5697F795489C8710BCC72141FF3675D53B@ex07.net.ucsf.edu> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1822F53B@RRMBX03.rrmc.local> I'm envious of you, Tim. I wanted the CoPath Plus system but unfortunately was shot down. Maybe one day... Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsfmedctr.org] Sent: Tuesday, March 11, 2014 12:51 PM To: 'Rathborne, Toni'; Joe W. Walker, Jr.; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: RE: Pathology Protocols Toni wrote: " I'm just curious about handwritten notes. We also have Cerner Millennium, but when I tried a few years ago to find out if there would be an impact in any of the areas, the OR reminded me that some of the surgeons like to draw diagrams for margins. The pathologists also document on the paper requisition when and who was called with frozen results. Although this is a small percentage of the specimens we receive, it stalled the project. How do you get around this?" We have Copath Plus (Cerner) and recently installed PicsPLus to capture all paperwork. Everything will be scanned into the case file for access later. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, March 11, 2014 9:34 AM To: 'Joe W. Walker, Jr.'; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Pathology Protocols Joe, I'm just curious about handwritten notes. We also have Cerner Millennium, but when I tried a few years ago to find out if there would be an impact in any of the areas, the OR reminded me that some of the surgeons like to draw diagrams for margins. The pathologists also document on the paper requisition when and who was called with frozen results. Although this is a small percentage of the specimens we receive, it stalled the project. How do you get around this? Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, March 11, 2014 12:21 PM To: Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Pathology Protocols Hi Karen, We utilize Cerner Millennium at my institution. We are 90% paper free. Fortunately for us, the vast majority of our surg path orders come to us as an electronic order, either placed by the provider on the floor or through an integrated office EMR. All op notes, office notes, radiology, etc are also available electronically to the pathologist at the time of the gross dictation. The gross dictation is transcribed into the surg path report prior to the pathologist getting their slides the next day. The pathologist uses either a bar code to scan the slide/accession number to bring up the surg path report with the transcribed gross dictation. The pathologist can again access all of the op notes, etc at the time of the final report's dictation. Also, all clinical test results are available within the application the pathologist uses to view the reports. Our transcriptionist manages the movement of cases to and from the pathologists' queue for their verification. The 10% of paper requisitions that we receive are ordered by our administrative staff where all of the written information on the paper is transferred to the electronic order. The paper reqs are then scanned into our system. Once in our system, the process for the paper reports is the same as the above. The exception is that we generally don't have office notes when we receive orders on paper. A couple of our pathologist do utilize a dual monitor set up that allows them to have the surg path report and the clinical info like radiology on a different monitor for their comparison. It didn't take a lot of convincing to get rid of the paper and has helped improve the accuracy of the orders and diagnostic information. There were definitely adjustments to everyone's workflows but we have been operating this way for 2 years now with very few problems. If you have the capability, I'd highly encourage removing the paper process, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, March 11, 2014 11:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology Protocols Importance: Low Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From vtobias <@t> uw.edu Tue Mar 11 12:00:32 2014 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Tue Mar 11 12:01:09 2014 Subject: [Histonet] RE: Pathology Protocols In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC1822F323@RRMBX03.rrmc.local> References: <6e55ab$8gr6v9@ironport10.mayo.edu> <3C2378778400AD448ADA6FD6BDB7CCCC1822F323@RRMBX03.rrmc.local> Message-ID: Joe, My question to you is, do you receive any paperwork with the specimen or just the specimen? If you have no paperwork, how are slides matched for delivery to the Pathologists? We use PowerPath and are probably in the 90% paperless also. Even with electronic orders, we still receive a printed copy of the order with the specimen. All paperwork is scanned into the case as an image at the time of accessioning. This takes care of any notes or drawings. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, March 11, 2014 9:21 AM To: Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Pathology Protocols Hi Karen, We utilize Cerner Millennium at my institution. We are 90% paper free. Fortunately for us, the vast majority of our surg path orders come to us as an electronic order, either placed by the provider on the floor or through an integrated office EMR. All op notes, office notes, radiology, etc are also available electronically to the pathologist at the time of the gross dictation. The gross dictation is transcribed into the surg path report prior to the pathologist getting their slides the next day. The pathologist uses either a bar code to scan the slide/accession number to bring up the surg path report with the transcribed gross dictation. The pathologist can again access all of the op notes, etc at the time of the final report's dictation. Also, all clinical test results are available within the application the pathologist uses to view the reports. Our transcriptionist manages the movement of cases to and from the pathologists' queue for their verification. The 10% of paper requisitions that we receive are ordered by our administrative staff where all of the written information on the paper is transferred to the electronic order. The paper reqs are then scanned into our system. Once in our system, the process for the paper reports is the same as the above. The exception is that we generally don't have office notes when we receive orders on paper. A couple of our pathologist do utilize a dual monitor set up that allows them to have the surg path report and the clinical info like radiology on a different monitor for their comparison. It didn't take a lot of convincing to get rid of the paper and has helped improve the accuracy of the orders and diagnostic information. There were definitely adjustments to everyone's workflows but we have been operating this way for 2 years now with very few problems. If you have the capability, I'd highly encourage removing the paper process, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, March 11, 2014 11:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology Protocols Importance: Low Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joewalker <@t> rrmc.org Tue Mar 11 12:19:04 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Mar 11 12:19:11 2014 Subject: [Histonet] RE: Pathology Protocols In-Reply-To: References: <6e55ab$8gr6v9@ironport10.mayo.edu> <3C2378778400AD448ADA6FD6BDB7CCCC1822F323@RRMBX03.rrmc.local> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1822F655@RRMBX03.rrmc.local> Hi Victor, For specimens that contain an electronic order, the specimen container is labeled with a spec label that is generated when the order is placed. The spec label has a Julian accession number on it that is unique to the electronic order. We do not have any paper orders with the specimen if the order was place from the office or floor electronically. When the spec is in the grossing lab, it is issued a unique surg path accession number, which also generates a label. This label is applied to the specimen container next to the Julian accession order label. The specimen is grossed under the surg path accession number, which is carried over to the cassette then to the slide. All of which are labeled from labels out of our system. The blocks are matched to the slide as they are cut, which are then stained with the label and finally delivered to the pathologist after coverslipping. Does that help answer your question? Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: Victor A. Tobias [mailto:vtobias@uw.edu] Sent: Tuesday, March 11, 2014 1:01 PM To: Joe W. Walker, Jr.; Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: RE: Pathology Protocols Joe, My question to you is, do you receive any paperwork with the specimen or just the specimen? If you have no paperwork, how are slides matched for delivery to the Pathologists? We use PowerPath and are probably in the 90% paperless also. Even with electronic orders, we still receive a printed copy of the order with the specimen. All paperwork is scanned into the case as an image at the time of accessioning. This takes care of any notes or drawings. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Tuesday, March 11, 2014 9:21 AM To: Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Pathology Protocols Hi Karen, We utilize Cerner Millennium at my institution. We are 90% paper free. Fortunately for us, the vast majority of our surg path orders come to us as an electronic order, either placed by the provider on the floor or through an integrated office EMR. All op notes, office notes, radiology, etc are also available electronically to the pathologist at the time of the gross dictation. The gross dictation is transcribed into the surg path report prior to the pathologist getting their slides the next day. The pathologist uses either a bar code to scan the slide/accession number to bring up the surg path report with the transcribed gross dictation. The pathologist can again access all of the op notes, etc at the time of the final report's dictation. Also, all clinical test results are available within the application the pathologist uses to view the reports. Our transcriptionist manages the movement of cases to and from the pathologists' queue for their verification. The 10% of paper requisitions that we receive are ordered by our administrative staff where all of the written information on the paper is transferred to the electronic order. The paper reqs are then scanned into our system. Once in our system, the process for the paper reports is the same as the above. The exception is that we generally don't have office notes when we receive orders on paper. A couple of our pathologist do utilize a dual monitor set up that allows them to have the surg path report and the clinical info like radiology on a different monitor for their comparison. It didn't take a lot of convincing to get rid of the paper and has helped improve the accuracy of the orders and diagnostic information. There were definitely adjustments to everyone's workflows but we have been operating this way for 2 years now with very few problems. If you have the capability, I'd highly encourage removing the paper process, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, March 11, 2014 11:25 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Pathology Protocols Importance: Low Hi all, Are there any AP labs that are totally paper free during slide diagnosis? We have the Vantage system with Sunquest CoPath. We still keep the paper specimen requisition with the specimen container during grossing. After digital gross dictation, the req slips are given to the transcriptionists. Transcriptionists type up the gross, print out patient histories, and place dictation and histories in a plastic sleeve. These case protocols are then brought back to Histology to be matched up with the slides. Slides are place in cardboard trays and matched up with the protocols. These are then placed in the pathologist slide area for the docs to pick up. We would really like to get rid of the paper protocols. Having Vantage, docs are able to scan the slides at their desks to bring up the patient information. Unfortunately, we are not doing this at this time. If there are any labs who are doing this, could you please tell me how your computer systems are set up? Are the specimen reqs scanned at accessioning? Do the docs have two monitors at their desks so they can view gross dictation and patient history at the same time? What did you do to essentially get rid of all the paper information? Any information that anyone can share with me is greatly appreciated. :) Thank you, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From billodonnell <@t> catholichealth.net Tue Mar 11 13:24:32 2014 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Mar 11 13:24:46 2014 Subject: [Histonet] Decal and molecular testing Message-ID: Can anyone recommend a decal solution that does no damage to specimens for molecular testing - or one that has minimal damage? Thanks - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From delsuec <@t> gmail.com Tue Mar 11 13:32:40 2014 From: delsuec <@t> gmail.com (Deloris Carter) Date: Tue Mar 11 13:32:42 2014 Subject: [Histonet] CEU's Message-ID: Can anyone recommend a website with free CEU's? Almost everything I find is for MLT, not HT. I appreciate any info. Deloris Carter, HT(ASCP) From wdesalvo.cac <@t> outlook.com Tue Mar 11 13:51:07 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Mar 11 13:51:11 2014 Subject: [Histonet] Decal and molecular testing In-Reply-To: References: Message-ID: To get the most DNA/RNA after decal you will want to stay away from HCL. There is the Immunocal that is Formic acid based (same company offers an EDTA solution) or Milestone Medical MOL-Decal 10 that is all EDTA. The issue is both will take longer to decal than HCL solutions. Fix the bone in 10% Formalin, time is dependant upon the size. Use stirring, heat, 50 degrees C, and pH 7.2-7.4. MOL-Decal 10 suggests 5-6 hrs for small biopsy bones and Immunocal 2-4 hrs. Try these and test at your site. Make sure you do a validation study to confirm results and use. Good luck William DeSalvo, BS HTL(ASCP) > From: billodonnell@catholichealth.net > To: histonet@lists.utsouthwestern.edu > Date: Tue, 11 Mar 2014 18:24:32 +0000 > Subject: [Histonet] Decal and molecular testing > > Can anyone recommend a decal solution that does no damage to specimens for molecular testing - or one that has minimal damage? Thanks - Bill > > > William (Bill) O'Donnell, HT (ASCP) QIHC > Senior Histologist > Good Samaritan Hospital > 10 East 31st Street > Kearney, NE 68847 > > SERENITY is not freedom from the storm, but peace amid the storm. > > Cultivate it in PRAYER! > > > > > > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Millard <@t> prlnet.com Tue Mar 11 14:38:17 2014 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Tue Mar 11 14:38:26 2014 Subject: [Histonet] Re: Pathology Protocols Message-ID: <64D11CD058311340A1966C234C6B0B893037669B@PRLEXCH02.prlnet.com> We are paperless. We use CoPath Plus and ImageNow. All of our requisitions (and any other paperwork for the case) are scanned into ImageNow Software. We distribute the slides on boards, and assign the cases in CoPath (put them into the Pathologists' worklists) at distribution. The pathologists scan the slide into CoPath Specimen field and it opens the case. We have an applications button for ImageNow, and while they are in the case in CoPath, they can click on that button, and it will bring up the requisition (or photos for gross-only, any accompanying paperwork they need to see). If they want, they can make annotations/ notes on the requisition. Once they finish with a specimen in CoPath, they click Next specimen and scan the next slide (the curser is automatically in the appropriate field for the scan). The pathologsists have wide-screen monitors. It wasn't a bump-free implementation, but is working fabulously. You need a champion on the pathologist side to help push it. I went out and trained all of the pathologists (we have 20, most of them off-site). Many were not excited about it, but they didn't have a choice (CEO and CMO championed and gave us the needed support). I think almost all of them now love it-better patient safety and a lot less paperwork piled on the desks, shelves...and saved about .5 FTE in the histology lab sorting, matching, stapling, and shuffling reports, requisitions and cases. Good luck! Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From lcolbert <@t> pathmdlabs.com Tue Mar 11 16:27:20 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Mar 11 16:30:13 2014 Subject: [Histonet] IHC Validation Message-ID: <12ECD7346266D74691EC2BFC75285E452F428996@BFL323E10.pathmdlabs.local> If I have validated an antibody at a specific incubation time, and then later want to decrease the incubation time, do I have to re-validate?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From santij1 <@t> bellsouth.net Tue Mar 11 19:07:20 2014 From: santij1 <@t> bellsouth.net (JERRY SANTIAGO) Date: Tue Mar 11 19:07:24 2014 Subject: [Histonet] Florida Society for Histotechnology 2014 Meeting Message-ID: <1394582840.8443.YahooMailNeo@web180902.mail.ne1.yahoo.com> The Florida Society for Histotechnology announces their 2014 meeting "Histotechnology, Let the Magic Begin! at the Buena Vista Palace Hotel & Spa in the majestic city of Orlando Florida (across the street from Downtown Disney). Dates: May 15 - 18, 2014. Outstanding Faculty and the New Supervisors & Administrators Academy: Phyllis Rutti Jennifer Roth Kaye Ryan Vinnie Della Speranza Loretta Sayles Liz Chlipala Bill De Salvo Joe Myers Theresa Burchette Jim Burchette Ada Feldman Ethel Macrea - Presenting the QIHC Readiness Workshop Farah Kahli Kathi Gizzi & Betsy Woessner - Presenting the HT Readiness Workshop Charity Event to benefit the Hubbard House in Orlando. The program and registration is available at www.fshgroup.org From dmcdowell <@t> GENERAL-DATA.com Wed Mar 12 05:18:18 2014 From: dmcdowell <@t> GENERAL-DATA.com (McDowell, Debbie) Date: Wed Mar 12 05:18:39 2014 Subject: [Histonet] Florida Society for Histotechnology 2014 Meeting In-Reply-To: <1394582840.8443.YahooMailNeo@web180902.mail.ne1.yahoo.com> References: <1394582840.8443.YahooMailNeo@web180902.mail.ne1.yahoo.com> Message-ID: <0D869FF1-5BC2-4892-9593-D555B467FAB9@general-data.com> Debbie McDowell, MSA, EMT-P Business Development Manager General Data Healthcare 484.885.3901 > On Mar 11, 2014, at 8:07 PM, "JERRY SANTIAGO" wrote: > > The Florida Society for Histotechnology announces their 2014 meeting "Histotechnology, Let the Magic Begin! at the Buena Vista Palace Hotel & Spa in the majestic city of Orlando Florida (across the street from Downtown Disney). > > Dates: May 15 - 18, 2014. > > Outstanding Faculty and the New Supervisors & Administrators Academy: > > Phyllis Rutti > Jennifer Roth > Kaye Ryan > Vinnie Della Speranza > Loretta Sayles > Liz Chlipala > Bill De Salvo > Joe Myers > Theresa Burchette > Jim Burchette > Ada Feldman > Ethel Macrea - Presenting the QIHC Readiness Workshop > > Farah Kahli > Kathi Gizzi & Betsy Woessner - Presenting the HT Readiness Workshop > > Charity Event to benefit the Hubbard House in Orlando. > > The program and registration is available at www.fshgroup.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email may contain confidential General Data Company, Inc. information: any unauthorized or improper disclosure, copying, distribution or use of the contents of this email and attached document(s) is prohibited and may be a criminal offense. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you received this email in error, please reply immediately to the sender & delete this message and the attached document(s) without disclosure. From tmcampbell <@t> fmh.org Wed Mar 12 05:49:38 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Wed Mar 12 05:49:46 2014 Subject: [Histonet] CEU's In-Reply-To: References: Message-ID: <3566D9E34287BE4B95372179009446A01B2BC149@EXCHANGE.fmhnt.fmh.org> Leica has free webinars monthly. And Medpage Today has some free CEU's and also try FreeCME Weekly. I just google free CEU's and hope to find stuff. You can use the MLT stuff also, because it is a related field. You only need 2 points in the area which you are certified. And 1 point in lab or patient safety. The remaining points can be in areas of specialty, management, education, or other related laboratory areas of interest. This is quoted from the ASCP website under requirements. Dako also has a few online education courses on their website. I am one that refuses to pay for any credits. Luckily, my employer has online educational courses for employees that I can take. You may want to check with your employer. Mine is a hospital and a lot of stuff and nurse related but there are things there that work. You may want to check with your employer. Hope this helps!! I too would also appreciate any other free websites/courses, so if you could forward any info you get to me, I would appreciate it!! Thanks. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Tuesday, March 11, 2014 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CEU's Can anyone recommend a website with free CEU's? Almost everything I find is for MLT, not HT. I appreciate any info. Deloris Carter, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Wed Mar 12 08:23:53 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Wed Mar 12 08:24:00 2014 Subject: [Histonet] RE: IHC Validation In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F428996@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F428996@BFL323E10.pathmdlabs.local> Message-ID: <196a3be102aa4662bae11f2f095bb209@BL2PR04MB196.namprd04.prod.outlook.com> On my IHC validation documents I have a space for comments. If I change anything in a protocol I make a note in the comments space. I haven't had any issue with any accrediting agencies. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Colbert Sent: Tuesday, March 11, 2014 7:27 PM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC Validation If I have validated an antibody at a specific incubation time, and then later want to decrease the incubation time, do I have to re-validate?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Wed Mar 12 08:33:32 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Mar 12 08:33:39 2014 Subject: [Histonet] Mississippi Society for Histotechnology 2014 Meeting In-Reply-To: <1394582840.8443.YahooMailNeo@web180902.mail.ne1.yahoo.com> References: <1394582840.8443.YahooMailNeo@web180902.mail.ne1.yahoo.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0112470EC0@JERRY.Gia.com> The Mississippi Society for Histotechnology meeting will be held in Hattiesburg, MS on Saturday, June 28th, 2014. All is welcome! If you have any questions, please feel free to contact me. Thanks! From TanyaAbbott <@t> catholichealth.net Wed Mar 12 08:37:06 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Mar 12 08:37:13 2014 Subject: [Histonet] Vented containers for microwave Message-ID: <852F7D2C14FB464D80E182B15DB138AF3065074A@CHIEX005.CHI.catholichealth.net> What sort of vented containers is everyone using for microwave Special stains? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From sbaldwin <@t> mhhcc.org Wed Mar 12 08:46:19 2014 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Wed Mar 12 08:46:27 2014 Subject: [Histonet] Performance Rounding questions for Histology Message-ID: Any one out there engaging in Baldridge Excellence program? We are going to start rounding (like physicians) with our employe and we need specific questions I need to ask. So I thought what be tter group to ask?? Histonetters!! I need safety, education, obstacle greatly app Thanks Histology/Cytology Supervisor S. Kathy Baldwi Memorial Hospital and Health Care Center sbaldwin@mhhcc Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Ce "Christ's healing mission of compassion empowers us through quality and excellence." From katelin09htl <@t> gmail.com Wed Mar 12 08:49:29 2014 From: katelin09htl <@t> gmail.com (Katelin Lester) Date: Wed Mar 12 08:49:35 2014 Subject: [Histonet] CEU's In-Reply-To: <3566D9E34287BE4B95372179009446A01B2BC149@EXCHANGE.fmhnt.fmh.org> References: <3566D9E34287BE4B95372179009446A01B2BC149@EXCHANGE.fmhnt.fmh.org> Message-ID: Sakura does one free webinar per month. http://www.sakura-americas.com/about/webinars.lasso Also, if you have a maintenance contact with some companies, such as Milestone Medical, they may a offer webinar series. Katelin, HTL On Mar 12, 2014 3:49 AM, "Campbell, Tasha M." wrote: > Leica has free webinars monthly. And Medpage Today has some free CEU's > and also try FreeCME Weekly. I just google free CEU's and hope to find > stuff. You can use the MLT stuff also, because it is a related field. > You only need 2 points in the area which you are certified. And 1 point > in lab or patient safety. The remaining points can be in areas of > specialty, management, education, or other related laboratory areas of > interest. This is quoted from the ASCP website under requirements. > Dako also has a few online education courses on their website. > > I am one that refuses to pay for any credits. Luckily, my employer has > online educational courses for employees that I can take. You may want > to check with your employer. Mine is a hospital and a lot of stuff and > nurse related but there are things there that work. You may want to > check with your employer. > > Hope this helps!! I too would also appreciate any other free > websites/courses, so if you could forward any info you get to me, I > would appreciate it!! > > Thanks. > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 (w) > 304-685-9307 (c) > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deloris > Carter > Sent: Tuesday, March 11, 2014 2:33 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CEU's > > Can anyone recommend a website with free CEU's? Almost everything I find > is > for MLT, not HT. > > I appreciate any info. > > Deloris Carter, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Donna.Willis <@t> baylorhealth.edu Wed Mar 12 09:11:48 2014 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Wed Mar 12 09:11:55 2014 Subject: [Histonet] RE: Vented containers for microwave In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF3065074A@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF3065074A@CHIEX005.CHI.catholichealth.net> Message-ID: <2572B4D63B62E64A8078D8BBE34D407801ABB914@BHDASVEXML2.bhcs.pvt> This will depend on the microwave brand that is being used. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Wednesday, March 12, 2014 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Vented containers for microwave What sort of vented containers is everyone using for microwave Special stains? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=5v0dVzZbzk%2BkkXJO3NUFH4br%2FdKQ3%2FGdsx0Tzyd19MA%3D%0A&s=e4c40f54b12cdbe3dd2d8368eb1916405b97787bacda3a04ab9202524abe6416 ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From gu.lang <@t> gmx.at Wed Mar 12 11:25:02 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Mar 12 11:25:29 2014 Subject: AW: [Histonet] Decal and molecular testing In-Reply-To: References: Message-ID: <000a01cf3e0f$9f85eea0$de91cbe0$@gmx.at> Hi, we had success with 5% formic acid on trephine bone marrow for DNA-PCR (BRAF, c-kit mutation) and RNA-ISH (kappa), but had no success for FISH-testing. Perhaps someone can recommend a method, that also supports FISH? Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von O'Donnell, Bill Gesendet: Dienstag, 11. M?rz 2014 19:25 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Decal and molecular testing Can anyone recommend a decal solution that does no damage to specimens for molecular testing - or one that has minimal damage? Thanks - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Justin.Peters <@t> hcahealthcare.com Wed Mar 12 11:36:58 2014 From: Justin.Peters <@t> hcahealthcare.com (Justin.Peters@hcahealthcare.com) Date: Wed Mar 12 11:37:04 2014 Subject: [Histonet] Xylene-free (isopropanol) processing protocol Message-ID: Is there anyone willing to share their processing protocol for using isopropanol without xylene? Justin Peters, HTL, QIHC (ASCP)CM Histology Supervisor Henrico Doctors' Hospital - Forest, Parham, and Retreat Campuses From Donna.Willis <@t> baylorhealth.edu Wed Mar 12 11:44:49 2014 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Wed Mar 12 11:45:09 2014 Subject: [Histonet] RE: Xylene-free (isopropanol) processing protocol In-Reply-To: References: Message-ID: <2572B4D63B62E64A8078D8BBE34D407801ABBABE@BHDASVEXML2.bhcs.pvt> What type of processing instrument are you using? Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Justin.Peters@hcahealthcare.com Sent: Wednesday, March 12, 2014 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene-free (isopropanol) processing protocol Is there anyone willing to share their processing protocol for using isopropanol without xylene? Justin Peters, HTL, QIHC (ASCP)CM Histology Supervisor Henrico Doctors' Hospital - Forest, Parham, and Retreat Campuses _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=ahR%2BEesJJIyPCeB0r09NvvXv7QXhwyB564nxDMSORZg%3D%0A&s=110740ac7328af42f3100dfaa8af79e8a4ef1ae569a4518813cb20146dd1bc8c ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From turkekul <@t> gmail.com Wed Mar 12 11:58:04 2014 From: turkekul <@t> gmail.com (Mesru T) Date: Wed Mar 12 12:03:24 2014 Subject: [Histonet] Alizarin Red on undecalcified bone Message-ID: Dear Histology Experts, I need your help. I am trying to stain undecalcified bone (adult mouse limb) with Alizarin Red S. I was thinking about two options: 1. Fixed frozen sections with Cryojane tape transfer system then staining the sections with Alizarin Red. 2. Alcohol fixed whole limb (whole mount preparation) stained with Alizarin Red then decalcified and processed for paraffin embedding/sectioning. I am not sure if the dye will survive decalcification and paraffin processing procedures. 3. I am trying to avoid doing plastic embedding unless there is no other option. Any insights are highly appreciated. Regards, Mesru From BZIMMERM <@t> gru.edu Wed Mar 12 12:23:57 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Wed Mar 12 12:24:03 2014 Subject: [Histonet] HISTOPALOOZA APRIL 25 - APRIL 27 GEORGIA SOCIETY FOR HISTOTECHNOLOGY Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8239DB@EX-MLB-03.ad.georgiahealth.edu> The Histopalooza is fast approaching and you need to secure your room at the Lodge and Spa at Callaway Gardens, Ga. If rooms are full, I suggest the cottages which are within steps of the Lodge. The contact person for securing your room is Taylor Reames 706-489-3359. I'm looking forward to some education, fun, networking, and peaceful tranquil gardens. I'm getting ready for the trip. I've already had WD-40 injected in my shoulder and knee. Got the duct tape packed for the trip, in case I need to give my knee some added support or I want to make a fake red tail light. I think I'm turning into the Tin Man from the Wizard of Oz. Look for me at Callaway, hope it doesn't rain or I will rust for sure. Ok, pass me the cheese to go with my whine. P.S. Wanda, I promise to behave. Here's a recap of speakers: H. Skip Brown Renal Pathology and Fixation Jim and Theresa Burchette IHC Vinnie Della Speranza Management ( and staying out of jail) Bill DeSalvo Tissue Processing/Lean/Six-Sigma Tools Lamar Jones Breast Pathology Wanda Jones Motivational Speaker (for all you Debbie Downers out there) Ely Klar Microscopic Identification Robert Lott HT/HTL Review Joe Myers QIHC review From oneilb <@t> wvuhealthcare.com Wed Mar 12 13:40:53 2014 From: oneilb <@t> wvuhealthcare.com (O'neil, Beth) Date: Wed Mar 12 13:41:04 2014 Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH Message-ID: <3CEB8EBCF9C7A648B9694B5696462A712880C322@NT-EXMB2.wvuh.wvuhs.com> Would fellow Histonetters be able to explain how they answer the following CAP question: ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. Where would one even find published benchmarks? Thank you Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals oneilb@wvuhealthcare.com 304-293-7629 (office) 304-293-6014 (lab) From pathology.histology <@t> gmail.com Wed Mar 12 14:12:12 2014 From: pathology.histology <@t> gmail.com (path lab) Date: Wed Mar 12 14:12:21 2014 Subject: [Histonet] retraction of mounting medium Message-ID: Has anyone experienced retraction of mounting medium from the edges of the slides (not yet reaching tissue but the retraction could conceivably continue until tissue is compromised) after long term (greater than 3 years) storage using recommended mounting media for automatic coverslippers? How have they dealt with this problem if it has occurred. From smcbride <@t> andrew.cmu.edu Wed Mar 12 14:39:22 2014 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Wed Mar 12 14:39:18 2014 Subject: [Histonet] Alizarin Red on undecalcified bone In-Reply-To: References: Message-ID: <4980D451-C0DF-4489-A461-009C66D936FE@andrew.cmu.edu> Hi Mesru, 1. Cryosectioning followed by Alizarin red staining may work. I'm not well-versed in this technique, so I will let other experts in this technique guide you. 2. Alizarin red functions by binding to calcium ions. Acids or chelating agents used in de-mineralization processes may very well cleave the alizarin-calcuim bond, resulting in loss of the differentiating stain. If time, materials and specimens are of a crucial nature, I would not recommend experimenting with this technique. 3. For years, I have worked with pmma embedded mineralized bone tissues with much success. There are a variety of stains to assist you in differentiation of various bone related structures. If you are not well versed with these techniques, there are labs, myself included, which can give you some pointers or contract to do the work for you. Best regards, Sean Sean McBride Senior Scientific Specialist Research Histology Services Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 571-989-BONE (m) 412-268-8275 (o) 412-268-8275 (fax) smcbride@andrew.cmu.edu > Dear Histology Experts, > > > I need your help. I am trying to stain undecalcified bone (adult mouse > limb) with Alizarin Red S. > I was thinking about two options: > 1. Fixed frozen sections with Cryojane tape transfer system then staining > the sections with Alizarin Red. > 2. Alcohol fixed whole limb (whole mount preparation) stained with Alizarin > Red then decalcified and processed for paraffin embedding/sectioning. I am > not sure if the dye will survive decalcification and paraffin processing > procedures. > 3. I am trying to avoid doing plastic embedding unless there is no other > option. > > Any insights are highly appreciated. > > Regards, > Mesru > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WaitT <@t> livemail.uthscsa.edu Wed Mar 12 14:57:21 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Wed Mar 12 14:57:39 2014 Subject: [Histonet] Paraffin type and Tetracycline labelling Questions Message-ID: <01932e7f5f2447f4a0889ced18392b1b@BL2PR01MB337.prod.exchangelabs.com> For those who have done Decalcified bone processing with paraffin....what is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry From shive003 <@t> umn.edu Wed Mar 12 15:39:04 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Mar 12 15:39:12 2014 Subject: [Histonet] Looking for PEDV antibody vendor Message-ID: Hello all, Can anyone suggest a stateside vendor for Porcine Epidemic Diarrhea Virus antibody? My overseas vendor is discontinuing their veterinary products and I'm in great need of a constant and reliable supply. Thanks in advance, -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From joewalker <@t> rrmc.org Wed Mar 12 15:40:22 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Wed Mar 12 15:40:33 2014 Subject: [Histonet] RE: Re: Pathology Protocols In-Reply-To: <64D11CD058311340A1966C234C6B0B893037669B@PRLEXCH02.prlnet.com> References: <64D11CD058311340A1966C234C6B0B893037669B@PRLEXCH02.prlnet.com> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1823257B@RRMBX03.rrmc.local> Donna brings up a good point. I too was able to remove a 0.5 FTE from my budget line once we went to a paperless system. This might help convince the pathologist to get on board with the paperless process. We did have a pathologist who helped user in the changes with the rest of the group. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Millard Sent: Tuesday, March 11, 2014 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Pathology Protocols We are paperless. We use CoPath Plus and ImageNow. All of our requisitions (and any other paperwork for the case) are scanned into ImageNow Software. We distribute the slides on boards, and assign the cases in CoPath (put them into the Pathologists' worklists) at distribution. The pathologists scan the slide into CoPath Specimen field and it opens the case. We have an applications button for ImageNow, and while they are in the case in CoPath, they can click on that button, and it will bring up the requisition (or photos for gross-only, any accompanying paperwork they need to see). If they want, they can make annotations/ notes on the requisition. Once they finish with a specimen in CoPath, they click Next specimen and scan the next slide (the curser is automatically in the appropriate field for the scan). The pathologsists have wide-screen monitors. It wasn't a bump-free implementation, but is working fabulously. You need a champion on the pathologist side to help push it. I went out and trained all of the pathologists (we have 20, most of them off-site). Many were not excited about it, but they didn't have a choice (CEO and CMO championed and gave us the needed support). I think almost all of them now love it-better patient safety and a lot less paperwork piled on the desks, shelves...and saved about .5 FTE in the histology lab sorting, matching, stapling, and shuffling reports, requisitions and cases. Good luck! Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From Steven.Weston <@t> utas.edu.au Wed Mar 12 17:02:31 2014 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Wed Mar 12 17:02:41 2014 Subject: [Histonet] TEM choices Message-ID: HI there fellow histonetters, We are in the fortunate position of being able to purchase a new TEM and was wondering what you all think of the present choices on market. We wish to get a digital machine to replace an ageing Joelle. Any feedback from users about recent purchases would be welcome, including ease of use, maintenance, support and reliability. regards Steve Weston Lab Manager Breathe-Well Centre of Research Excellence for Chronic Respiratory Disease. UTAS-SOM 0408990859 62264871 From SSCALISE <@t> beaumont.edu Wed Mar 12 21:59:38 2014 From: SSCALISE <@t> beaumont.edu (Sharon Scalise) Date: Wed Mar 12 21:59:47 2014 Subject: [Histonet] RE: Cornflaking artifact Message-ID: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu> I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffin....what is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Thu Mar 13 07:35:10 2014 From: b427297 <@t> aol.com (b427297@aol.com) Date: Thu Mar 13 07:35:14 2014 Subject: [Histonet] RE: Cornflaking artifact In-Reply-To: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu> References: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu> Message-ID: <71432615-FE06-4F2E-A20F-C500A948819B@aol.com> Is this a tape coverslipper? If so, you do have minute traces of water carryover to your xylene. If there is even the hint of pink in you last dehydrant before xylene, you will get that cornflake artifact. Acetone wont help, because water still be present. Increase number of absolute alcohol before xylene, and check often for eosin carryover. This fixed our problem. Jackie O' Sent from my iPhone > On Mar 12, 2014, at 21:59, Sharon Scalise wrote: > > I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP!!!!! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan > Sent: Wednesday, March 12, 2014 3:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Paraffin type and Tetracycline labelling Questions > > For those who have done Decalcified bone processing with paraffin....what is the best type of paraffin that you guys are familiar with? > > Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Thu Mar 13 08:29:52 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Thu Mar 13 08:33:02 2014 Subject: [Histonet] RE: Cornflaking artifact In-Reply-To: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu> References: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu> Message-ID: <12ECD7346266D74691EC2BFC75285E452F428C8A@BFL323E10.pathmdlabs.local> You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffin....what is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis <@t> che-east.org Thu Mar 13 08:53:58 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu Mar 13 08:53:34 2014 Subject: [Histonet] MUM-1 Message-ID: Good morning Histonet Folks, I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the "usual adjustments" and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this. Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From SHEILA.HERRINGTON <@t> interiorhealth.ca Thu Mar 13 10:42:00 2014 From: SHEILA.HERRINGTON <@t> interiorhealth.ca (HERRINGTON, SHEILA) Date: Thu Mar 13 10:42:14 2014 Subject: [Histonet] RE: Cornflaking artifact In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F428C8A@BFL323E10.pathmdlabs.local> References: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu> <12ECD7346266D74691EC2BFC75285E452F428C8A@BFL323E10.pathmdlabs.local> Message-ID: <9D5F3F245A5FE0458E46E9A71E3EE08C06DE6D8766@DC1SERV352.interiorhealth.ca> We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffin....what is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srishan <@t> mail.holyname.org Thu Mar 13 10:59:06 2014 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Thu Mar 13 10:59:13 2014 Subject: [Histonet] RE: Cornflaking artifact In-Reply-To: <9D5F3F245A5FE0458E46E9A71E3EE08C06DE6D8766@DC1SERV352.interiorhealth.ca> References: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu> <12ECD7346266D74691EC2BFC75285E452F428C8A@BFL323E10.pathmdlabs.local> <9D5F3F245A5FE0458E46E9A71E3EE08C06DE6D8766@DC1SERV352.interiorhealth.ca> Message-ID: We had this problem several years ago. We were using the sakura tapes with the coverslipper. We did the following: Last three alcohols were changes frequently. Slides should be not dry when loading on coverslipper. If you could load two racks at a time, only load one. By this way the slides in the second rack will not dry out. Finally, change the tapes from sakura to Mercedes Medical tapes. Mala Nirmala Srishan Holy Name Medical Center From: "HERRINGTON, SHEILA" To: 'Laurie Colbert' , Sharon Scalise , "histonet@lists.utsouthwestern.edu" Date: 03/13/2014 11:43 AM Subject: [Histonet] RE: Cornflaking artifact Sent by: histonet-bounces@lists.utsouthwestern.edu We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffin....what is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. 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From kenneth.metzger <@t> aruplab.com Thu Mar 13 11:13:24 2014 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Thu Mar 13 11:13:33 2014 Subject: [Histonet] RE: Cornflaking artifact In-Reply-To: <9D5F3F245A5FE0458E46E9A71E3EE08C06DE6D8766@DC1SERV352.interiorhealth.ca> References: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu> <12ECD7346266D74691EC2BFC75285E452F428C8A@BFL323E10.pathmdlabs.local> <9D5F3F245A5FE0458E46E9A71E3EE08C06DE6D8766@DC1SERV352.interiorhealth.ca> Message-ID: <3855827CD3E36249A30D57F6F896F8F193EFCAE5@EXMBX2.aruplab.net> Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing this as well out of the blue..haven't changed a thing. Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, SHEILA Sent: Thursday, March 13, 2014 9:42 AM To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffin....what is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From LSebree <@t> uwhealth.org Thu Mar 13 11:19:11 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Mar 13 11:19:16 2014 Subject: [Histonet] RE: MUM-1 In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF09D462@UWHC-MBX12.uwhis.hosp.wisc.edu> This is our protocol for the Ultra; maybe it will help. 64" CC1, 32" incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4". This is with UltraView DAB detection. We use tonsil as well however we validated with HD, LN, GI, tonsil, etc. Good luck! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Thursday, March 13, 2014 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MUM-1 Good morning Histonet Folks, I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the "usual adjustments" and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this. Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Thu Mar 13 11:21:40 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu Mar 13 11:21:46 2014 Subject: [Histonet] RE: Cornflaking artifact In-Reply-To: <3855827CD3E36249A30D57F6F896F8F193EFCAE5@EXMBX2.aruplab.net> References: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu>, <12ECD7346266D74691EC2BFC75285E452F428C8A@BFL323E10.pathmdlabs.local>, <9D5F3F245A5FE0458E46E9A71E3EE08C06DE6D8766@DC1SERV352.interiorhealth.ca>, <3855827CD3E36249A30D57F6F896F8F193EFCAE5@EXMBX2.aruplab.net> Message-ID: Long time and high volume user of the Sakura Tape. Check the Xylene drops. We coverslip thousands of slides daily and have seen no isssue with the tape lots. Our experience shows some cornflaking when the slide does not receive enough xylens to wet the slide properly. William DeSalvo, BS HTL(ASCP) > From: kenneth.metzger@aruplab.com > To: SHEILA.HERRINGTON@interiorhealth.ca; lcolbert@pathmdlabs.com; SSCALISE@beaumont.edu; histonet@lists.utsouthwestern.edu > Date: Thu, 13 Mar 2014 16:13:24 +0000 > CC: > Subject: [Histonet] RE: Cornflaking artifact > > Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing this as well out of the blue..haven't changed a thing. > > Kenneth G Metzger HTL(ASCP) > Histology Supervisor > ARUP Labs > Salt Lake City, Utah > Phone: (801)583-2787 ext. 3101 > Fax: (801) 584-5244 > Email: kenneth.metzger@aruplab.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, SHEILA > Sent: Thursday, March 13, 2014 9:42 AM > To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > > We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. > > > Sheila Herrington > Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital > 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 > 250-862-4300 ext 7587 or 7510 > Sheila.herrington@interiorhealth.ca > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Thursday, March 13, 2014 6:30 AM > To: Sharon Scalise; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > > You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. > > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise > Sent: Wednesday, March 12, 2014 8:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > > I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP!!!!! > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan > Sent: Wednesday, March 12, 2014 3:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Paraffin type and Tetracycline labelling Questions > > For those who have done Decalcified bone processing with paraffin....what is the best type of paraffin that you guys are familiar with? > > Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------- > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.Lavigne <@t> sphp.com Thu Mar 13 11:29:45 2014 From: Lisa.Lavigne <@t> sphp.com (Lavigne, Lisa) Date: Thu Mar 13 11:30:23 2014 Subject: [Histonet] RE: Cornflaking artifact In-Reply-To: <3855827CD3E36249A30D57F6F896F8F193EFCAE5@EXMBX2.aruplab.net> References: <190D98228ADC1747BCE27019B78FD8F3011DA03B@EXMAIL06.ms.beaumont.edu> <12ECD7346266D74691EC2BFC75285E452F428C8A@BFL323E10.pathmdlabs.local> <9D5F3F245A5FE0458E46E9A71E3EE08C06DE6D8766@DC1SERV352.interiorhealth.ca>, <3855827CD3E36249A30D57F6F896F8F193EFCAE5@EXMBX2.aruplab.net> Message-ID: <7A037867E212C441A97961C00B766A051509316E05@CHEXCMS13.one.ads.che.org> We have changed lots of tape and still are having this artifact. Lisa LaVigne CT, MB (ASCP) Pathology Manager St. Peter's Health Partners 315 S. Manning Blvd. Albany, NY 12208 Phone: 518-525-5274 Fax: 518-525-6750 Email: Lisa.LaVigne@sphp.com ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth [kenneth.metzger@aruplab.com] Sent: Thursday, March 13, 2014 12:13 PM To: HERRINGTON, SHEILA; 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing this as well out of the blue..haven't changed a thing. Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger@aruplab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, SHEILA Sent: Thursday, March 13, 2014 9:42 AM To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffin....what is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From michele.french <@t> bms.com Thu Mar 13 11:43:19 2014 From: michele.french <@t> bms.com (French, Michele) Date: Thu Mar 13 11:43:34 2014 Subject: [Histonet] New Jersey Meeting Message-ID: <1A7E70D2A397674BA82830AAB9C5020D52FAED7FAC@ushpwbmsmmp006.one.ads.bms.com> I just wanted to send a reminder that this Friday, March 15th is the registration deadline for the NJSH Spring Meeting planned for April 5th in Mount Laurel, NJ. There will be four 90 minute seminars entitled "Respiratory Histopathology", "Pathology of the Urinary Tract", "Animal Model Development to Aid in the Understanding of Human Atherosclerosis", and "Predictive Testing in Non-small Cell Lung Cancer: Testing Protocols, Specimen Requirements and Near Future Techniques, Notably Next Generation Sequencing". The cost is ONLY $90 for the day and you will receive 6 CEUs for attending! For more information and to register on-line, please visit our website http://njsh.org/njsh/ ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From TGoins <@t> mt.gov Thu Mar 13 12:16:52 2014 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Mar 13 12:18:02 2014 Subject: [Histonet] IHC without wash buffer Message-ID: In a continuing effort to limit the volume of reagent used in each step of a manual IHC, I have tried TBS and TBST on slides with barriers (expensive) and without barriers. The results were not stellar with the barrier slides - the reagent still escapes. We dewax with hot detergent (may be a contributing factor) and the Tween 20 in the TBST definitely alters the hydrophobicity of the barrier, an effect that is not reversed with a water wash. Consequently, I have resorted to omitting the buffer wash steps and using distilled water only. The slide surface remains "water repellent" and the added IHC reagents form a pool over the tissue sections. The detection method is a polymer-HRP and there is no increase in background staining in the tissue or on the slide surface. I am assuming that the IHC reagents are prepared in the "optimum" suspension liquid and a buffer is not required. So, I am interested in hearing from anyone why this is a bad idea. Thanks, Tresa [X] [X] From sarahlkp <@t> comcast.net Thu Mar 13 12:41:02 2014 From: sarahlkp <@t> comcast.net (sarah holmes) Date: Thu Mar 13 12:41:23 2014 Subject: [Histonet] request for surplus equipment Message-ID: <5321EDAE.3030100@comcast.net> Dear Histonet, A colleague in Manicaland Province, Mutare, Zimbabwe is in need of a thermocycler if anyone has an old one that they would be able to donate. The colleague is the Coordinator for Laboratory Training at Africa University.She and the university work to provide opportunities for students from all over Africa to learn clinical laboratory science skills. Please contact me off list if you can help and I will arrange shipping and put you in contact with the university! Thank you, -- Sarah Holmes Laboratory Manager Laboratory for Kidney Pathology, Inc. 1916 Patterson St, Suite 501 Nashville, TN 37203 615 321 5729 From suetp918 <@t> comcast.net Thu Mar 13 13:01:20 2014 From: suetp918 <@t> comcast.net (Sue) Date: Thu Mar 13 13:01:46 2014 Subject: [Histonet] RE: Cornflaking artifact In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F428C8A@BFL323E10.pathmdlabs.local> Message-ID: <558169345.599707.1394733680495.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> We had this issue when we were using the tape from Mercedes Medical and went back to Sakura tape and it went away.? It could also be associated with humidity.? STP TJUH From tbraud <@t> holyredeemer.com Thu Mar 13 13:06:23 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Mar 13 13:06:28 2014 Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH In-Reply-To: <20140313155058.C5F3E1E8087@trendmess-svr.holyredeemer.local> References: <20140313155058.C5F3E1E8087@trendmess-svr.holyredeemer.local> Message-ID: Try calling CAP. They provided the benchmarks we use for our annual statistics for ER/PR. I don't have the recent CAP checklist handy, but on the 9.25.2012 checklist, the benchmarks for ER/PR are published in the notes section of the question. I hope this helps. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 3 Date: Wed, 12 Mar 2014 18:40:53 +0000 From: "O'neil, Beth" Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH Would fellow Histonetters be able to explain how they answer the following CAP question: ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. Where would one even find published benchmarks? Thank you Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals oneilb@wvuhealthcare.com 304-293-7629 (office) 304-293-6014 (lab) ********************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From bethcoxx <@t> gmail.com Thu Mar 13 13:09:41 2014 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Thu Mar 13 13:09:46 2014 Subject: [Histonet] Re: Cornflaking Message-ID: Sharon & other Histonetters, Cornflaking is literally microscopic air trapped under the coverslip. It doesn't have anything to do with poor dehydration and trapped water. Thus it can be caused by partial drying out before the coverslipping. The things you need to look at to eradicate the problem is: 1. Keep the slides wet before coverslipping (obviously) 2. Consider increasing the amount of xylene deposited on the slide for tape coverslippers. 3. For glass coverslippers, consider increasing the xylene and increasing or changing the mounting media. Cornflaking tends to happen more on slides/sections with rough "topography" on the section (the more rough it is, the more nooks & crannies to trap the air). So anything that would give the section a more rough surface would increase the tendency to cornflake; such as: section lifting. section thickness, even chatter in the sections. Think about things that would affect the section - for example, are you using a different brand of blade on the microtome? Hope this helps, Beth Cox, HTL/SCT(ASCP)QIHC Message: 15 Date: Thu, 13 Mar 2014 11:59:06 -0400 From: srishan@mail.holyname.org Subject: Re: [Histonet] RE: Cornflaking artifact To: "HERRINGTON, SHEILA" Cc: "histonet@lists.utsouthwestern.edu" , Sharon Scalise , histonet-bounces@lists.utsouthwestern.edu, 'Laurie Colbert' Message-ID: < OF46844F54.2F7FC911-ON85257C9A.00575CB9-85257C9A.0057CEBC@holyname.org> Content-Type: text/plain; charset="US-ASCII" We had this problem several years ago. We were using the sakura tapes with the coverslipper. We did the following: Last three alcohols were changes frequently. Slides should be not dry when loading on coverslipper. If you could load two racks at a time, only load one. By this way the slides in the second rack will not dry out. Finally, change the tapes from sakura to Mercedes Medical tapes. Mala Nirmala Srishan Holy Name Medical Center From: "HERRINGTON, SHEILA" To: 'Laurie Colbert' , Sharon Scalise , "histonet@lists.utsouthwestern.edu" Date: 03/13/2014 11:43 AM Subject: [Histonet] RE: Cornflaking artifact Sent by: histonet-bounces@lists.utsouthwestern.edu We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 Sheila.herrington@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP!!!!! From powderhound34 <@t> hotmail.com Thu Mar 13 13:19:17 2014 From: powderhound34 <@t> hotmail.com (joe joe) Date: Thu Mar 13 13:19:21 2014 Subject: [Histonet] Re: Cornflaking In-Reply-To: References: Message-ID: Why doesn't someone call Kellogg's ? > On Mar 13, 2014, at 12:10 PM, "Beth Cox" wrote: > > Sharon & other Histonetters, > > Cornflaking is literally microscopic air trapped under the coverslip. It > doesn't have anything to do with poor dehydration and trapped water. Thus > it can be caused by partial drying out before the coverslipping. > > The things you need to look at to eradicate the problem is: > 1. Keep the slides wet before coverslipping (obviously) > 2. Consider increasing the amount of xylene deposited on the slide for > tape coverslippers. > 3. For glass coverslippers, consider increasing the xylene and increasing > or changing the mounting media. > > Cornflaking tends to happen more on slides/sections with rough "topography" > on the section (the more rough it is, the more nooks & crannies to trap the > air). So anything that would give the section a more rough surface would > increase the tendency to cornflake; such as: section lifting. section > thickness, even chatter in the sections. Think about things that would > affect the section - for example, are you using a different brand of blade > on the microtome? > > Hope this helps, > > Beth Cox, HTL/SCT(ASCP)QIHC > > > > > Message: 15 > Date: Thu, 13 Mar 2014 11:59:06 -0400 > From: srishan@mail.holyname.org > Subject: Re: [Histonet] RE: Cornflaking artifact > To: "HERRINGTON, SHEILA" > Cc: "histonet@lists.utsouthwestern.edu" > , Sharon Scalise > , > histonet-bounces@lists.utsouthwestern.edu, > 'Laurie Colbert' > Message-ID: > < > OF46844F54.2F7FC911-ON85257C9A.00575CB9-85257C9A.0057CEBC@holyname.org> > Content-Type: text/plain; charset="US-ASCII" > We had this problem several years ago. We were using the sakura tapes > with the coverslipper. > We did the following: > Last three alcohols were changes frequently. > Slides should be not dry when loading on coverslipper. > If you could load two racks at a time, only load one. By this way the > slides in the second rack will not dry out. > Finally, change the tapes from sakura to Mercedes Medical tapes. > Mala > Nirmala Srishan > Holy Name Medical Center > > > From: "HERRINGTON, SHEILA" > To: 'Laurie Colbert' , Sharon Scalise > , "histonet@lists.utsouthwestern.edu" > > Date: 03/13/2014 11:43 AM > Subject: [Histonet] RE: Cornflaking artifact > Sent by: histonet-bounces@lists.utsouthwestern.edu > > We also have recently started to see this artifact more than ever before, > and nothing in our process has changed. We have tried everything to > correct to no avail. Wonder if it is possible to be a change in some type > of supply, either xylene or coverslipping film. Something has changed but > am at a loss as to what. > > Sheila Herrington > Technical Lead Histopathology and Immunohistochemistry > Kelowna General Hospital > 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 > 250-862-4300 ext 7587 or 7510 > Sheila.herrington@interiorhealth.ca > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [ > mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Laurie > Colbert > Sent: Thursday, March 13, 2014 6:30 AM > To: Sharon Scalise; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > You will also see the cornflaking if your tissue is lifting off of the > slide at all. We used to get this more often on hard, decal specimens > than on other specimens. We used the film to coverslip. If you remove > the film from the problem slides and recoverslip conventionally with extra > mountant and glass coverslips, I'm sure you will not see the artifact. > Laurie Colbert > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [ > mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Sharon > Scalise > Sent: Wednesday, March 12, 2014 8:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > I am looking for help with "cornflaking" (tiny, brown dry spots under > coverslip)artifact. We have been using fresh xylene on our stainer and > coverslipper, cleaned and wiped all containers dry before filling, tried > different lots of coverslipping film and had service on our coverslipper > to make sure it was functioning properly, including the xylene drip. We > continue to have this artifact and it is driving us crazy. It is sporadic > with no pattern of tissue type or placement on the slide. Sometimes it > lands on tissue other times not. Most of the time when we remove the > coverslip and re-coverslip it goes away (I am assuming because the acetone > removes any minute amounts of water that may be present). We just cannot > figure out where the water is coming from. Has anyone seen this artifact > while using the drying step on the prisma stainer? We just recently > started using the drying on some slides and I am thinking maybe it is > causing humidity??? I cannot say for a fact that our "cornflaking" > started at the same time, but it is suspicious. HELP!!!!! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From imhyper13 <@t> aol.com Thu Mar 13 13:29:35 2014 From: imhyper13 <@t> aol.com (imhyper13@aol.com) Date: Thu Mar 13 13:29:38 2014 Subject: [Histonet] GI Biopsies Message-ID: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> Good afternoon all, I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. Thank you From srishan <@t> mail.holyname.org Thu Mar 13 13:31:37 2014 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Thu Mar 13 13:31:43 2014 Subject: [Histonet] Re: Cornflaking In-Reply-To: References: Message-ID: We are all putting out what worked for us. You should try some of these suggestions and see what works for you. The suggestion for improving poor dehydration was suggested by technical support from Sakura. Cornflaking was irritating so many of our pathologist, we had to get technical support from Sakura to see how this could be resolved. In OUR hospital changing the tape worked!!! Good Luck.!!! Mala Nirmala Srishan Holy Name Medical Center. From: joe joe To: Beth Cox Cc: "histonet@lists.utsouthwestern.edu" Date: 03/13/2014 02:20 PM Subject: Re: [Histonet] Re: Cornflaking Sent by: histonet-bounces@lists.utsouthwestern.edu Why doesn't someone call Kellogg's ? > On Mar 13, 2014, at 12:10 PM, "Beth Cox" wrote: > > Sharon & other Histonetters, > > Cornflaking is literally microscopic air trapped under the coverslip. It > doesn't have anything to do with poor dehydration and trapped water. Thus > it can be caused by partial drying out before the coverslipping. > > The things you need to look at to eradicate the problem is: > 1. Keep the slides wet before coverslipping (obviously) > 2. Consider increasing the amount of xylene deposited on the slide for > tape coverslippers. > 3. For glass coverslippers, consider increasing the xylene and increasing > or changing the mounting media. > > Cornflaking tends to happen more on slides/sections with rough "topography" > on the section (the more rough it is, the more nooks & crannies to trap the > air). So anything that would give the section a more rough surface would > increase the tendency to cornflake; such as: section lifting. section > thickness, even chatter in the sections. Think about things that would > affect the section - for example, are you using a different brand of blade > on the microtome? > > Hope this helps, > > Beth Cox, HTL/SCT(ASCP)QIHC > > > > > Message: 15 > Date: Thu, 13 Mar 2014 11:59:06 -0400 > From: srishan@mail.holyname.org > Subject: Re: [Histonet] RE: Cornflaking artifact > To: "HERRINGTON, SHEILA" > Cc: "histonet@lists.utsouthwestern.edu" > , Sharon Scalise > , > histonet-bounces@lists.utsouthwestern.edu, > 'Laurie Colbert' > Message-ID: > < > OF46844F54.2F7FC911-ON85257C9A.00575CB9-85257C9A.0057CEBC@holyname.org> > Content-Type: text/plain; charset="US-ASCII" > We had this problem several years ago. We were using the sakura tapes > with the coverslipper. > We did the following: > Last three alcohols were changes frequently. > Slides should be not dry when loading on coverslipper. > If you could load two racks at a time, only load one. By this way the > slides in the second rack will not dry out. > Finally, change the tapes from sakura to Mercedes Medical tapes. > Mala > Nirmala Srishan > Holy Name Medical Center > > > From: "HERRINGTON, SHEILA" > To: 'Laurie Colbert' , Sharon Scalise > , "histonet@lists.utsouthwestern.edu" > > Date: 03/13/2014 11:43 AM > Subject: [Histonet] RE: Cornflaking artifact > Sent by: histonet-bounces@lists.utsouthwestern.edu > > We also have recently started to see this artifact more than ever before, > and nothing in our process has changed. We have tried everything to > correct to no avail. Wonder if it is possible to be a change in some type > of supply, either xylene or coverslipping film. Something has changed but > am at a loss as to what. > > Sheila Herrington > Technical Lead Histopathology and Immunohistochemistry > Kelowna General Hospital > 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 > 250-862-4300 ext 7587 or 7510 > Sheila.herrington@interiorhealth.ca > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [ > mailto:histonet-bounces@lists.utsouthwestern.edu ] > On Behalf Of Laurie > Colbert > Sent: Thursday, March 13, 2014 6:30 AM > To: Sharon Scalise; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > You will also see the cornflaking if your tissue is lifting off of the > slide at all. We used to get this more often on hard, decal specimens > than on other specimens. We used the film to coverslip. If you remove > the film from the problem slides and recoverslip conventionally with extra > mountant and glass coverslips, I'm sure you will not see the artifact. > Laurie Colbert > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [ > mailto:histonet-bounces@lists.utsouthwestern.edu ] > On Behalf Of Sharon > Scalise > Sent: Wednesday, March 12, 2014 8:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > I am looking for help with "cornflaking" (tiny, brown dry spots under > coverslip)artifact. We have been using fresh xylene on our stainer and > coverslipper, cleaned and wiped all containers dry before filling, tried > different lots of coverslipping film and had service on our coverslipper > to make sure it was functioning properly, including the xylene drip. We > continue to have this artifact and it is driving us crazy. It is sporadic > with no pattern of tissue type or placement on the slide. Sometimes it > lands on tissue other times not. Most of the time when we remove the > coverslip and re-coverslip it goes away (I am assuming because the acetone > removes any minute amounts of water that may be present). We just cannot > figure out where the water is coming from. Has anyone seen this artifact > while using the drying step on the prisma stainer? We just recently > started using the drying on some slides and I am thinking maybe it is > causing humidity??? I cannot say for a fact that our "cornflaking" > started at the same time, but it is suspicious. HELP!!!!! > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From tbraud <@t> holyredeemer.com Thu Mar 13 13:34:20 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Mar 13 13:34:31 2014 Subject: [Histonet] RE: Cornflakes In-Reply-To: <20140313155058.C5F3E1E8087@trendmess-svr.holyredeemer.local> References: <20140313155058.C5F3E1E8087@trendmess-svr.holyredeemer.local> Message-ID: As a long time user of film (though sadly, no longer) we used to see what sounds like this artifact when the Xylene dispenser was not dispensing enough xylene and the slides were not wet enough. It drove us crazy until we realized that through the years, the xylene dispensing knob had become very loose and would readjust itself at the slightest touch or vibration. We liked our Xylene to be dispensing rapidly, just short of a steady stream, so constantly checked it. Also, if your slides are not completely dehydrated before clearing in Xylene, you are just asking for problems, if not now, then years down the road when the tape will pull off the slide, taking your tissue with it. Been there, done that. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 10 Date: Thu, 13 Mar 2014 02:59:38 +0000 From: Sharon Scalise Subject: [Histonet] RE: Cornflaking artifact To: "histonet@lists.utsouthwestern.edu" I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Joyce.Weems <@t> emoryhealthcare.org Thu Mar 13 13:36:21 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Mar 13 13:36:34 2014 Subject: [Histonet] GI Biopsies In-Reply-To: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> References: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> Message-ID: We do 3 levels on one slide. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of imhyper13@aol.com Sent: Thursday, March 13, 2014 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies Good afternoon all, I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From suetp918 <@t> comcast.net Thu Mar 13 13:36:31 2014 From: suetp918 <@t> comcast.net (Sue) Date: Thu Mar 13 13:36:42 2014 Subject: [Histonet] GI Biopsies In-Reply-To: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> Message-ID: <1953863326.600821.1394735791761.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> We actually cut 2 slides with 4 levels 2 on each slide.? Out pathologists were unwilling to only cut one slide From joelleweaver <@t> hotmail.com Thu Mar 13 13:36:42 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 13 13:36:45 2014 Subject: [Histonet] RE: MUM-1 In-Reply-To: <77DD817201982748BC67D7960F2F76AF09D462@UWHC-MBX12.uwhis.hosp.wisc.edu> References: , <77DD817201982748BC67D7960F2F76AF09D462@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: I have the bond and I use the BIO SB concentrate, RaBMab, EP190 at 1:50 and it stains great on tonsil and kidney tubules. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: LSebree@uwhealth.org > To: CDavis@che-east.org; histonet@lists.utsouthwestern.edu > Date: Thu, 13 Mar 2014 16:19:11 +0000 > CC: > Subject: [Histonet] RE: MUM-1 > > This is our protocol for the Ultra; maybe it will help. > > 64" CC1, 32" incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4". This is with UltraView DAB detection. We use tonsil as well however we validated with HD, LN, GI, tonsil, etc. > > Good luck! > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > Sent: Thursday, March 13, 2014 8:54 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] MUM-1 > > Good morning Histonet Folks, > > I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the "usual adjustments" and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this. > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Mar 13 13:43:20 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 13 13:43:28 2014 Subject: [Histonet] GI Biopsies In-Reply-To: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> References: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E391681C1EE22@IBMB7Exchange.digestivespecialists.com> 3 levels - 1 slide -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of imhyper13@aol.com Sent: Thursday, March 13, 2014 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies Good afternoon all, I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Mar 13 13:44:37 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 13 13:44:41 2014 Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH In-Reply-To: References: <20140313155058.C5F3E1E8087@trendmess-svr.holyredeemer.local>, Message-ID: are you looking for the stats in the notes for ANP. 22970 (2012) ? "overall ER negative breast ca ( invasive DCIS) should not exceed 30% ( lower average 20-35% in post menopausal) , lower in well differentiated tumors...etc." I sure CAP can also send or repeat them to you if you prefer to call them. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Thu, 13 Mar 2014 14:06:23 -0400 > From: tbraud@holyredeemer.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH > > Try calling CAP. They provided the benchmarks we use for our annual > statistics for ER/PR. I don't have the recent CAP checklist handy, but > on the 9.25.2012 checklist, the benchmarks for ER/PR are published in > the notes section of the question. I hope this helps. Sincerely, Terri > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > Message: 3 > Date: Wed, 12 Mar 2014 18:40:53 +0000 > From: "O'neil, Beth" > Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH > Would fellow Histonetters be able to explain how they answer the > following CAP question: > ANP.22970 For immunohistochemical and FISH/ISH tests that provide > independent predictive information, the laboratory at least annually > compares its patient results with published benchmarks, and evaluates > interobserver variability among the pathologists in the laboratory. > Where would one even find published benchmarks? Thank you > > Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC > Histology Supervisor/Technical Specialist > West Virginia University Hospitals > oneilb@wvuhealthcare.com > 304-293-7629 (office) > 304-293-6014 (lab) > > ********************************** > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it was sent. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Mar 13 13:48:10 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 13 13:48:14 2014 Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH In-Reply-To: References: <20140313155058.C5F3E1E8087@trendmess-svr.holyredeemer.local>, , , Message-ID: sorry , saw the Predictive, missed FISH - getting ready to do this myself. I would just call CAP, if I get to do that soon I will send along what information they supply. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: joelleweaver@hotmail.com > To: tbraud@holyredeemer.com; histonet@lists.utsouthwestern.edu > Date: Thu, 13 Mar 2014 18:44:37 +0000 > Subject: RE: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH > CC: > > are you looking for the stats in the notes for ANP. 22970 (2012) ? > > "overall ER negative breast ca ( invasive DCIS) should not exceed 30% ( lower average 20-35% in post menopausal) , lower in well differentiated tumors...etc." > > I sure CAP can also send or repeat them to you if you prefer to call them. > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > Date: Thu, 13 Mar 2014 14:06:23 -0400 > > From: tbraud@holyredeemer.com > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH > > > > Try calling CAP. They provided the benchmarks we use for our annual > > statistics for ER/PR. I don't have the recent CAP checklist handy, but > > on the 9.25.2012 checklist, the benchmarks for ER/PR are published in > > the notes section of the question. I hope this helps. Sincerely, Terri > > > > Terri L. Braud, HT(ASCP) > > Anatomic Pathology Supervisor > > Holy Redeemer Hospital Laboratory > > 1648 Huntingdon Pike > > Meadowbrook, PA 19046 > > Ph: 215-938-3676 > > Fax: 215-938-3874 > > > > Message: 3 > > Date: Wed, 12 Mar 2014 18:40:53 +0000 > > From: "O'neil, Beth" > > Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH > > Would fellow Histonetters be able to explain how they answer the > > following CAP question: > > ANP.22970 For immunohistochemical and FISH/ISH tests that provide > > independent predictive information, the laboratory at least annually > > compares its patient results with published benchmarks, and evaluates > > interobserver variability among the pathologists in the laboratory. > > Where would one even find published benchmarks? Thank you > > > > Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC > > Histology Supervisor/Technical Specialist > > West Virginia University Hospitals > > oneilb@wvuhealthcare.com > > 304-293-7629 (office) > > 304-293-6014 (lab) > > > > ********************************** > > --------------------------------------------------------------------------------- > > > > > > > > CONFIDENTIALITY NOTICE: > > > > This E-Mail is intended only for the use of the individual or entity to which > > it was sent. It may contain information that is privileged and/or confidential, > > and the use or disclosure of such information may also be restricted under applicable > > federal and state law. If you received this communication in error, please do not > > distribute any part of it or retain any copies, and delete the original E-Mail. > > Please notify the sender of any error by E-Mail. > > > > Thank you for your cooperation. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 <@t> gmail.com Thu Mar 13 15:38:37 2014 From: cls71877 <@t> gmail.com (Cristi Rigazio) Date: Thu Mar 13 15:38:48 2014 Subject: [Histonet] GI Biopsies In-Reply-To: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> References: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> Message-ID: <35006E0F-8255-4275-95D4-DEC3A19FC1A1@gmail.com> Three levels one slide for all biopsies. Colon polyps one section. Sent from my iPhone On Mar 13, 2014, at 11:29 AM, imhyper13@aol.com wrote: > > Good afternoon all, > I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. > Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Patterson <@t> propath.com Thu Mar 13 18:58:32 2014 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Thu Mar 13 18:58:37 2014 Subject: [Histonet] IHC Opening Dallas Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D167D965B@Mail.propathlab.com> IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 1 - 2 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 2:00 p.m. to 10:30 p.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE M/F/Disabled/Veteran Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com. Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From tina.vanmeter <@t> gmail.com Thu Mar 13 22:18:15 2014 From: tina.vanmeter <@t> gmail.com (Tina Van Meter) Date: Thu Mar 13 22:18:23 2014 Subject: [Histonet] Leica CM3600 Cryomacrotome XP Message-ID: Hello Histonetters, Is anyone currently or in the past had experience operating the Leica CM3600 Cryomacrotome XP? I am looking for input on using this instrument. Thanks, Tina From Susan.Walzer <@t> HCAHealthcare.com Fri Mar 14 02:01:11 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Mar 14 02:01:22 2014 Subject: [Histonet] GI Biopsies In-Reply-To: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> References: <8D10D015174A010-2F78-D313@webmail-d155.sysops.aol.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FB41462F8@FWDCWPMSGCMS09.hca.corpad.net> We do 3 levels on all..taking hp immuno on 2nd level of gastrics and a couple of extra on esophs. ( in case of alcian blues or pas/fungus). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of imhyper13@aol.com Sent: Thursday, March 13, 2014 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies Good afternoon all, I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KGoodkowsky <@t> goodwin.edu Fri Mar 14 06:47:26 2014 From: KGoodkowsky <@t> goodwin.edu (Kelli Goodkowsky) Date: Fri Mar 14 06:47:34 2014 Subject: [Histonet] Microwave Tissue Processing Message-ID: Hello all, I had an opportunity to demo a microwave tissue processing unit for my students. Is anyone using microwave technology for tissue processing and if so, could you please provide me some information on your experience with this? There are many pros that I can see, including its ease of use and quick processing time which fits well with the student lab schedule. I am wondering, however, what the likelihood will be that students will use this technology once in the field. I don't want to do them a disservice by not using conventional tissue processing methods. The majority of hospitals in the CT/MA area use conventional tissue processors. Thank you. Sent from my iPad Kelli Goodkowsky Director Clinical Education, Histologic Science Goodwin College (860) 727-6917 From joelleweaver <@t> hotmail.com Fri Mar 14 07:02:40 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Mar 14 07:02:43 2014 Subject: [Histonet] Microwave Tissue Processing In-Reply-To: References: Message-ID: I have used a couple of vendor's MW processing instruments over the past 8-10 years. So it is used, even if it has not become as commonplace as conventional in every setting or market. It seems to be more favored in high volume settings, for pretty obvious reasons. In teaching and instruction * my opinion * - you should teach them the theory and fundamentals for practice for ALL the possible tissue processing technologies they may encounter, and this is consistent with the approach to practice of the topics on the ASCP exam.They have to know the fundamental basics and then it is easy to expand to more emerging practices and technology. It would be more of a disservice to me if you left anything( either conventional technology or MW out), in your treatment of that topic. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: KGoodkowsky@goodwin.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 14 Mar 2014 11:47:26 +0000 > Subject: [Histonet] Microwave Tissue Processing > > Hello all, > I had an opportunity to demo a microwave tissue processing unit for my students. Is anyone using microwave technology for tissue processing and if so, could you please provide me some information on your experience with this? There are many pros that I can see, including its ease of use and quick processing time which fits well with the student lab schedule. I am wondering, however, what the likelihood will be that students will use this technology once in the field. I don't want to do them a disservice by not using conventional tissue processing methods. The majority of hospitals in the CT/MA area use conventional tissue processors. > > Thank you. > > Sent from my iPad > Kelli Goodkowsky > Director Clinical Education, Histologic Science > Goodwin College > (860) 727-6917 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ahc53 <@t> georgetown.edu Fri Mar 14 07:18:21 2014 From: ahc53 <@t> georgetown.edu (Anna Coffey) Date: Fri Mar 14 07:18:26 2014 Subject: [Histonet] Job at Georgetown University in Washington, DC Message-ID: Hi Histonetters, We still have a job opening for a histotech at Georgetown University in Washington, DC. We think an ideal candidate might be a recent student or tech looking to gain experience for their ASCP certification. I realize the posted salary range starts pretty low, but we are aiming to hire someone at the higher end. Additionally, the posting says the applicant must have 1-3 years experience and be eligible for their certification, but we are considering applicants with less experience at this point. If you know of anyone that might be interested (even if they think they may not be qualified), please pass this posting along and have them contact me if they have any questions at all. The posting is here: http://www12.georgetown.edu/hr/employment_services/joblist/job_description.cfm?CategoryID=7&RequestNo=20140338 Thanks! Anna -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu From badams <@t> acadianagastro.com Fri Mar 14 07:27:49 2014 From: badams <@t> acadianagastro.com (=?utf-8?b?QnJlbnQgQWRhbXM=?=) Date: Fri Mar 14 07:27:52 2014 Subject: [Histonet] GI Biopsies Message-ID: <20140314072749.ljpcfxzhss4okk04@webmail.windstreamhosting.com> We do three (3) levels of two (2) sections on one (1) slide. Most pathologist and Histotechs like this as it gives the Pathologist everything he needs to see in order to make an accurate diagnosis and reduces the number of slides he must view. Brent D. Adams -BS, LPN, HT Acadiana Gastroenterology Associates, LLC Histology Lab 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-0963 MAIN fax: (337) 269-0553 LAB fax: (337) 408-1250 www.acadianagastro.com PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original along with any attachments. Any other use of the email is strictly prohibited. From rsrichmond <@t> gmail.com Fri Mar 14 07:37:46 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Mar 14 07:37:48 2014 Subject: [Histonet] Re: GI biopsies Message-ID: An anonymous query: >>I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment.<< Well, I take what I can get. Many histotechs lack the skill, or are unwilling to lay more than one ribbon on a slide. I do like more than one level. A more serious problem is maintaining the quality of GI biopsy sections, one of the most difficult quality assurance issues in histopathology. (It was reviewed in J HIstotechnol last year - I can find the reference.) The problem is at its worst with duodenal biopsies, where some services never prepare an adequate slide. As the "celiac disease" fad spreads and bread is the Evil Food of the Year, I am really concerned about signing out duodenal biopsies where I can't even distinguish the lymphocytes. Edwards Deming lives! Bob Richmond Samurai Pathologist Maryville TN From madelinegi <@t> yahoo.com Fri Mar 14 08:57:13 2014 From: madelinegi <@t> yahoo.com (Madeline Gi) Date: Fri Mar 14 08:57:16 2014 Subject: [Histonet] GI Biopsies In-Reply-To: <20140314072749.ljpcfxzhss4okk04@webmail.windstreamhosting.com> References: <20140314072749.ljpcfxzhss4okk04@webmail.windstreamhosting.com> Message-ID: <1394805433.43661.YahooMailNeo@web161003.mail.bf1.yahoo.com> I work in a GI lab we cut one slide with four sections the first two sections are placed on the top half of the slide then turn ten more and then add it to the first two sections. If recut's are required we pick up the first few sections depending on the pathoIogist request. I hope this helps.? ? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com On Friday, March 14, 2014 8:28 AM, Brent Adams wrote: We do three (3) levels of two (2) sections on one (1) slide. Most pathologist and Histotechs like this as it gives the Pathologist everything he needs to see in order to make an accurate diagnosis and reduces the number of slides he must view. Brent D. Adams -BS, LPN, HT Acadiana Gastroenterology Associates, LLC Histology Lab 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-0963 MAIN fax: (337) 269-0553 LAB fax: (337) 408-1250 www.acadianagastro.com PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original along with any attachments. Any other use of the email is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smirnof1966 <@t> gmail.com Fri Mar 14 12:38:32 2014 From: smirnof1966 <@t> gmail.com (smirnof1966@gmail.com) Date: Fri Mar 14 12:38:37 2014 Subject: [Histonet] Microwave Tissue Processing Message-ID: Hi Kelli we use MW tissue processor KOS And Pathos milestone. I don t like rapid processing because of high temperature in protocols. So we use KOS only for GI biopsy or gross hardening ( autopsy brain). Pathos we used for surgical matherial and bone marrow processing. Smirnof Dmitry chief department anatomy pathology Russia sankt Petersburg ?????????? ? iPad From ttroyer <@t> petersonlab.com Fri Mar 14 12:40:39 2014 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Fri Mar 14 12:40:48 2014 Subject: [Histonet] (no subject) Message-ID: <119CE3DA2B7A4FF691924FE3D375C919@Peterson.local> We are needing to dispose of patient slides and blocks that are beyond the years that we need to keep them. What have people found is the safest and most economical way to do this? Thanks, Travis Troyer Histology Supervisor Peterson Laboratory Services Manhattan, KS From joelleweaver <@t> hotmail.com Fri Mar 14 13:02:18 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Mar 14 13:02:23 2014 Subject: [Histonet] Re: GI biopsies In-Reply-To: References: Message-ID: Yes Dr. Richmond GI biopsies are prone to processing issues and shatter/chatter artifact. I like to put three true levels on one slide with unstained for later SS & IHC , OR put two parallel ribbons on one slide, ( 2 slides of 2 ribbons, for 4 actual levels). I put three ribbons for Hirshsprungs on each slide to provide the section numbers without making multitudes of slides. I have a hard time getting this accepted- The pathologist almost always wants three ribbons on 2-3 slides, and I think that is because only some of the sections are truly "readable"- the section quality is too variable for these specimens for them to feel comfortable. I like to reveiw these under the microscope since when they are tiny, it is hard to see the shatter, folds and fragmentation on the water bath. I agree it is definately a quality problem to be addressed. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 14 Mar 2014 08:37:46 -0400 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: GI biopsies > > An anonymous query: >>I was just curious about how your institutions handle > GI biopsies, specifically how many slides you cut off the bat. We presently > cut 2 levels on each GI biopsy block, but I'm hearing that more and more > places only cut 1 slide per GI biopsy block. Please share what you are > doing at your establishment.<< > > Well, I take what I can get. Many histotechs lack the skill, or are > unwilling to lay more than one ribbon on a slide. I do like more than one > level. > > A more serious problem is maintaining the quality of GI biopsy sections, > one of the most difficult quality assurance issues in histopathology. (It > was reviewed in J HIstotechnol last year - I can find the reference.) The > problem is at its worst with duodenal biopsies, where some services never > prepare an adequate slide. As the "celiac disease" fad spreads and bread is > the Evil Food of the Year, I am really concerned about signing out duodenal > biopsies where I can't even distinguish the lymphocytes. > > Edwards Deming lives! > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHargrove <@t> unitedregional.org Fri Mar 14 13:29:54 2014 From: SHargrove <@t> unitedregional.org (Susie Hargrove) Date: Fri Mar 14 13:30:00 2014 Subject: [Histonet] RE: GI Biopsies Message-ID: We also cut 3 levels 2 sections each level on one slide. And we all lay out and pick up ribbons in the exact same order. The deepest (last) cut is always at the top (label end) . ________________________________ Susie Hargrove HT (ASCP) Histology Technical Specialist United Regional Health Care Wichita Falls, Texas 76301 Ph 940-764-3881 Fax-940-764-3129 ________________________________ ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Friday, March 14, 2014 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 124, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Microwave Tissue Processing (joelle weaver) 2. Job at Georgetown University in Washington, DC (Anna Coffey) 3. GI Biopsies ( Brent Adams ) 4. Re: GI biopsies (Bob Richmond) 5. Re: GI Biopsies (Madeline Gi) ---------------------------------------------------------------------- Message: 1 Date: Fri, 14 Mar 2014 12:02:40 +0000 From: joelle weaver Subject: RE: [Histonet] Microwave Tissue Processing To: Kelli Goodkowsky , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have used a couple of vendor's MW processing instruments over the past 8-10 years. So it is used, even if it has not become as commonplace as conventional in every setting or market. It seems to be more favored in high volume settings, for pretty obvious reasons. In teaching and instruction * my opinion * - you should teach them the theory and fundamentals for practice for ALL the possible tissue processing technologies they may encounter, and this is consistent with the approach to practice of the topics on the ASCP exam.They have to know the fundamental basics and then it is easy to expand to more emerging practices and technology. It would be more of a disservice to me if you left anything( either conventional technology or MW out), in your treatment of that topic. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: KGoodkowsky@goodwin.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 14 Mar 2014 11:47:26 +0000 > Subject: [Histonet] Microwave Tissue Processing > > Hello all, > I had an opportunity to demo a microwave tissue processing unit for my students. Is anyone using microwave technology for tissue processing and if so, could you please provide me some information on your experience with this? There are many pros that I can see, including its ease of use and quick processing time which fits well with the student lab schedule. I am wondering, however, what the likelihood will be that students will use this technology once in the field. I don't want to do them a disservice by not using conventional tissue processing methods. The majority of hospitals in the CT/MA area use conventional tissue processors. > > Thank you. > > Sent from my iPad > Kelli Goodkowsky > Director Clinical Education, Histologic Science > Goodwin College > (860) 727-6917 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 14 Mar 2014 08:18:21 -0400 From: Anna Coffey Subject: [Histonet] Job at Georgetown University in Washington, DC To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters, We still have a job opening for a histotech at Georgetown University in Washington, DC. We think an ideal candidate might be a recent student or tech looking to gain experience for their ASCP certification. I realize the posted salary range starts pretty low, but we are aiming to hire someone at the higher end. Additionally, the posting says the applicant must have 1-3 years experience and be eligible for their certification, but we are considering applicants with less experience at this point. If you know of anyone that might be interested (even if they think they may not be qualified), please pass this posting along and have them contact me if they have any questions at all. The posting is here: http://www12.georgetown.edu/hr/employment_services/joblist/job_description.cfm?CategoryID=7&RequestNo=20140338 Thanks! Anna -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu ------------------------------ Message: 3 Date: Fri, 14 Mar 2014 07:27:49 -0500 From: " Brent Adams " Subject: [Histonet] GI Biopsies To: histonet@lists.utsouthwestern.edu Message-ID: <20140314072749.ljpcfxzhss4okk04@webmail.windstreamhosting.com> Content-Type: text/plain; charset=UTF-8 We do three (3) levels of two (2) sections on one (1) slide. Most pathologist and Histotechs like this as it gives the Pathologist everything he needs to see in order to make an accurate diagnosis and reduces the number of slides he must view. Brent D. Adams -BS, LPN, HT Acadiana Gastroenterology Associates, LLC Histology Lab 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-0963 MAIN fax: (337) 269-0553 LAB fax: (337) 408-1250 www.acadianagastro.com PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original along with any attachments. Any other use of the email is strictly prohibited. ------------------------------ Message: 4 Date: Fri, 14 Mar 2014 08:37:46 -0400 From: Bob Richmond Subject: [Histonet] Re: GI biopsies To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 An anonymous query: >>I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment.<< Well, I take what I can get. Many histotechs lack the skill, or are unwilling to lay more than one ribbon on a slide. I do like more than one level. A more serious problem is maintaining the quality of GI biopsy sections, one of the most difficult quality assurance issues in histopathology. (It was reviewed in J HIstotechnol last year - I can find the reference.) The problem is at its worst with duodenal biopsies, where some services never prepare an adequate slide. As the "celiac disease" fad spreads and bread is the Evil Food of the Year, I am really concerned about signing out duodenal biopsies where I can't even distinguish the lymphocytes. Edwards Deming lives! Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 5 Date: Fri, 14 Mar 2014 06:57:13 -0700 (PDT) From: Madeline Gi Subject: Re: [Histonet] GI Biopsies To: Brent Adams , "histonet@lists.utsouthwestern.edu" Message-ID: <1394805433.43661.YahooMailNeo@web161003.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I work in a GI lab we cut one slide with four sections the first two sections are placed on the top half of the slide then turn ten more and then add it to the first two sections. If recut's are required we pick up the first few sections depending on the pathoIogist request. I hope this helps.? ? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com On Friday, March 14, 2014 8:28 AM, Brent Adams wrote: We do three (3) levels of two (2) sections on one (1) slide. Most pathologist and Histotechs like this as it gives the Pathologist everything he needs to see in order to make an accurate diagnosis and reduces the number of slides he must view. Brent D. Adams -BS, LPN, HT Acadiana Gastroenterology Associates, LLC Histology Lab 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-0963 MAIN fax: (337) 269-0553 LAB fax: (337) 408-1250 www.acadianagastro.com PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original along with any attachments. Any other use of the email is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 124, Issue 15 ***************************************** From carl.hobbs <@t> kcl.ac.uk Fri Mar 14 13:32:50 2014 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Fri Mar 14 13:32:55 2014 Subject: [Histonet] Re: Alizarin Red on undecalcified bone Message-ID: ? ? ? ? ? Strip off all soft tissue ( if you don't need it) Fix ( in Bancroft and Stevens the method uses alcohol but, we have used Formalin pH7). Follow the Tripp and MacKay method. ? ? ? ? ? ? Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813????????? From barbara.tibbs <@t> accuratediagnosticlabs.com Fri Mar 14 13:35:27 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Fri Mar 14 13:35:33 2014 Subject: [Histonet] Re: GI biopsies In-Reply-To: References: Message-ID: Dr. Richmond, A large portion of our business is GI biopsies. We cut three levels per slide. We achieve this by cutting three ribbons at different levels and picking up two sections from each ribbon. If an H.pylori or AB/PAS is ordered we choose two sections from the middle ribbon. I check the quality of the slides before handing them out to the pathologists. I encourage the pathologists to share any unhappiness they have with our microtoming and work to improve the problem ASAP. It seems to me that skilled, caring histotechnologists plus good communication with the pathologists is the magic equation for excellent quality slides. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bob Richmond Sent: Friday, March 14, 2014 10:37 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: GI biopsies An anonymous query: >>I was just curious about how your institutions handle GI biopsies, specifically how many slides you cut off the bat. We presently cut 2 levels on each GI biopsy block, but I'm hearing that more and more places only cut 1 slide per GI biopsy block. Please share what you are doing at your establishment.<< Well, I take what I can get. Many histotechs lack the skill, or are unwilling to lay more than one ribbon on a slide. I do like more than one level. A more serious problem is maintaining the quality of GI biopsy sections, one of the most difficult quality assurance issues in histopathology. (It was reviewed in J HIstotechnol last year - I can find the reference.) The problem is at its worst with duodenal biopsies, where some services never prepare an adequate slide. As the "celiac disease" fad spreads and bread is the Evil Food of the Year, I am really concerned about signing out duodenal biopsies where I can't even distinguish the lymphocytes. Edwards Deming lives! Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Fri Mar 14 14:31:48 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Mar 14 14:31:54 2014 Subject: [Histonet] Re: GI biopsies In-Reply-To: References: Message-ID: I prefer to put 3 true levels (2 sections off of 3 different ribbons, 50-60 um between levels, given adequate tissue size), picking up the sections horizontally on the slide. In this way you get 6 diagnostic sections on one slide. Saves space on the stainer. I find you really have to stress adequate hydration/cooling of the blocks to avoid artifact, especially in a lab where the bxs are not run on a separate processor/ protocol. This doesn't mean you can leave blocks floating in your icetray while you go to lunch. But a good 5-10 mins on ice really helps. Also, I find I physically slow down my microtome stroke a little when cutting GI bxs, and cut nice long ribbons. The sections in the middle of a long ribbon will exhibit very little variability in thickness. When you see a slide with 3 sections on it, each of a different thickness, it's usually the result of an inexperienced or rushed tech cutting 3-4 section "ribbons" without allowing for adequate cooling/hydration time. I have also frequently QC'd slides in which the first slide is good, but the next 2 levels progressively deteriorate. This is due to inadequate hydration/cooling between levels. Again, it behooves everyone to really slow down and take your time cutting GI bxs. On Fri, Mar 14, 2014 at 1:35 PM, Barbara Tibbs < barbara.tibbs@accuratediagnosticlabs.com> wrote: > Dr. Richmond, > > A large portion of our business is GI biopsies. We cut three levels per > slide. We achieve this by cutting three ribbons at different levels and > picking up two sections from each ribbon. If an H.pylori or AB/PAS is > ordered we choose two sections from the middle ribbon. I check the quality > of the slides before handing them out to the pathologists. I encourage the > pathologists to share any unhappiness they have with our microtoming and > work to improve the problem ASAP. > > It seems to me that skilled, caring histotechnologists plus good > communication with the pathologists is the magic equation for excellent > quality slides. > > Barbara S. Tibbs > Histology Supervisor > Accurate Diagnostic Labs > South Plainfield, NJ > barbara.tibbs@accuratediagnosticlabs.com > 732-839-3374 > Cell: 610-809-6508 > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu < > histonet-bounces@lists.utsouthwestern.edu> on behalf of Bob Richmond < > rsrichmond@gmail.com> > Sent: Friday, March 14, 2014 10:37 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: GI biopsies > > An anonymous query: >>I was just curious about how your institutions handle > GI biopsies, specifically how many slides you cut off the bat. We presently > cut 2 levels on each GI biopsy block, but I'm hearing that more and more > places only cut 1 slide per GI biopsy block. Please share what you are > doing at your establishment.<< > > Well, I take what I can get. Many histotechs lack the skill, or are > unwilling to lay more than one ribbon on a slide. I do like more than one > level. > > A more serious problem is maintaining the quality of GI biopsy sections, > one of the most difficult quality assurance issues in histopathology. (It > was reviewed in J HIstotechnol last year - I can find the reference.) The > problem is at its worst with duodenal biopsies, where some services never > prepare an adequate slide. As the "celiac disease" fad spreads and bread is > the Evil Food of the Year, I am really concerned about signing out duodenal > biopsies where I can't even distinguish the lymphocytes. > > Edwards Deming lives! > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Richard.Cartun <@t> hhchealth.org Fri Mar 14 15:01:54 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Mar 14 15:01:59 2014 Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH In-Reply-To: <3CEB8EBCF9C7A648B9694B5696462A712880C322@NT-EXMB2.wvuh.wvuhs.com> References: <3CEB8EBCF9C7A648B9694B5696462A712880C322@NT-EXMB2.wvuh.wvuhs.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E02289AC0@HHCEXCHMB03.hhcsystem.org> I'm not aware of published benchmarks for FISH/ISH, but if you're doing IHC for ER, PR, and HER2 in breast CA you may find the following information useful: Lal P, et al: ER and PR and histologic features in 3,655 invasive breast carcinomas. Am J Clin Pathol 2005;123:541-546. ER+ tumors - 74% PR+ tumors - 49 HER2+ tumors - 16% Fitzgibbons PL, et al: Recommendations for validating ER and PR IHC assays. Arch Pathol Lab Med 2010;134:930-935. For women over 65 years of age, the % of negative cases should not exceed 20%. For "low-grade" invasive carcinomas, the proportion of negative cases should not exceed 5%. My own data for invasive breast CA: ER+ tumors - 85% PR+ tumors - 70% HER2+ tumors - 14% Please keep in mind that with the introduction of new monoclonal antibodies, more sensitive detection systems, and the "recommendation" that tumors with "1%" immunoreactive cells be called "Positive", the "old" benchmarks for ER and PR are no longer valid. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'neil, Beth Sent: Wednesday, March 12, 2014 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH Would fellow Histonetters be able to explain how they answer the following CAP question: ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. Where would one even find published benchmarks? Thank you Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals oneilb@wvuhealthcare.com 304-293-7629 (office) 304-293-6014 (lab) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From jennifer.harvey <@t> Vanderbilt.Edu Fri Mar 14 15:12:11 2014 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Fri Mar 14 15:12:19 2014 Subject: [Histonet] Block holder for old Thermo Fisher Cryotome E cryostat Message-ID: Does anyone out there had an old Thermo Fisher Cryotome E crostat? I need a block holder. The spring in ours broke. Fisher dosnet have any in stock-3 week wait! I have tried Biosurplus and Belair, and Southeast Pathology. No luck. Any other suggestions? I have a call into IMEB but no answer yet. Thanks! Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 From Glenn.Hauck <@t> albertahealthservices.ca Fri Mar 14 16:47:24 2014 From: Glenn.Hauck <@t> albertahealthservices.ca (Glenn Hauck) Date: Fri Mar 14 16:47:32 2014 Subject: [Histonet] RE: Fungus Controls In-Reply-To: <6e55ab$8d6oon@ironport10.mayo.edu> References: <6e55ab$8d6oon@ironport10.mayo.edu> Message-ID: <38FA8FCA474F834CB9B90590D7C72390015AFE71F232@EXMBX4C.crha.bewell.ca> We have grown our own using orange peels. Once the fungus is growing on the peel we process it just like any other tissue. Fungus turns out great. Glenn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Thursday, February 20, 2014 1:57 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Fungus Controls Importance: Low Hi all, We are in need of some Fungus controls. Anyone have any extras to spare? Will do a trade... if we have anything available for you. Thank you very much, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From sjschwendinger <@t> aol.com Fri Mar 14 23:25:10 2014 From: sjschwendinger <@t> aol.com (Sarah Schwendinger) Date: Fri Mar 14 23:25:16 2014 Subject: [Histonet] RE: Fungus Controls In-Reply-To: <38FA8FCA474F834CB9B90590D7C72390015AFE71F232@EXMBX4C.crha.bewell.ca> References: <6e55ab$8d6oon@ironport10.mayo.edu> <38FA8FCA474F834CB9B90590D7C72390015AFE71F232@EXMBX4C.crha.bewell.ca> Message-ID: <047E2DD0-0BC4-4A18-8151-37D6A16E4C39@aol.com> I work in a Vet. Pathology lab and we like to use Bree cheese rind and Blue cheese veins as our fungus controls. They process great. Sarah Sent from my iPad On Mar 14, 2014, at 2:47 PM, Glenn Hauck wrote: > We have grown our own using orange peels. Once the fungus is growing on the peel we process it just like any other tissue. Fungus turns out great. > > Glenn > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. > Sent: Thursday, February 20, 2014 1:57 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Fungus Controls > Importance: Low > > Hi all, > > We are in need of some Fungus controls. Anyone have any extras to spare? Will do a trade... if we have anything available for you. > > Thank you very much, > > Karen > > Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Mar 15 16:22:03 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Mar 15 16:22:08 2014 Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E02289AC0@HHCEXCHMB03.hhcsystem.org> References: <3CEB8EBCF9C7A648B9694B5696462A712880C322@NT-EXMB2.wvuh.wvuhs.com>, <9215BD4B0BA1B44D962A71C758B68D2E02289AC0@HHCEXCHMB03.hhcsystem.org> Message-ID: Thank you Dr. Cartun, these numbers are helpful for reference Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Richard.Cartun@hhchealth.org > To: oneilb@wvuhealthcare.com; histonet@lists.utsouthwestern.edu > Date: Fri, 14 Mar 2014 20:01:54 +0000 > CC: > Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH > > I'm not aware of published benchmarks for FISH/ISH, but if you're doing IHC for ER, PR, and HER2 in breast CA you may find the following information useful: > > Lal P, et al: ER and PR and histologic features in 3,655 invasive breast carcinomas. Am J Clin Pathol 2005;123:541-546. > > ER+ tumors - 74% > PR+ tumors - 49 > HER2+ tumors - 16% > > > Fitzgibbons PL, et al: Recommendations for validating ER and PR IHC assays. Arch Pathol Lab Med 2010;134:930-935. > > For women over 65 years of age, the % of negative cases should not exceed 20%. > For "low-grade" invasive carcinomas, the proportion of negative cases should not exceed 5%. > > > My own data for invasive breast CA: > > ER+ tumors - 85% > PR+ tumors - 70% > HER2+ tumors - 14% > > > Please keep in mind that with the introduction of new monoclonal antibodies, more sensitive detection systems, and the "recommendation" that tumors with "1%" immunoreactive cells be called "Positive", the "old" benchmarks for ER and PR are no longer valid. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'neil, Beth > Sent: Wednesday, March 12, 2014 2:41 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH > > Would fellow Histonetters be able to explain how they answer the following CAP question: > ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. > Where would one even find published benchmarks? Thank you > > Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC > Histology Supervisor/Technical Specialist West Virginia University Hospitals oneilb@wvuhealthcare.com > 304-293-7629 (office) > 304-293-6014 (lab) > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sat Mar 15 22:21:32 2014 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Mar 15 22:21:40 2014 Subject: [Histonet] (no subject) Message-ID: Hi Tresa, I haven't seen any responses to this question, so I figure I should take a crack at it. You are right that using a polymer detection system is so clean that you hardly have much to worry about background/non-specific staining when not using a buffer for wash steps. There is however a good reason to use them anyway. To explain this it might help to think of two slinkey springs laying parallel to each other. You need to get them to slide together as perfectly as possible. If they are moving around like slinkeys always do, this will be a difficult thing to accomplish. You will need to get them to sit still so that when you push them together they will just slide nicely into place. Taking this analogy to the realm of IHC that we are discussing, the two slinkeys are obviously the antibody and antigen. The wiggling and jiggling that they are doing is due to Van der Walls forces (I probably misspelled that). Now we usually have a few strategies to make these jiggley antibodies behave. Having a good antibody diluent helps a lot, as does having an antibody that has a high affinity and avidity to the antigen. It also helps to have the keep the reaction at a constant pH and tonicity. That is where the wash buffer comes in. It can keep the non-specific staining out when using a avidin-biotin reaction, but it also makes the antigen-antibody reaction more comfortable. So, yes you will surely see some decent results with distilled water washes especially with antibodies that are really attracted to each other, but for the ones that need some more coaxing or the ones that you are *really* depending on specificity for quantitation (her2, ER, PR etc), you will really want to make sure you have given these two love bird slinkeys as much chance to hook up a possible. I hate trying to explain something like this without citing a good source for where I got this from. In looking around there isn't much out there explaining the "Why" of many IHC procedures. So much of modern medicine is voodoo just follow the procedure, dont ask questions! This is why I always recommend the Dako IHC manuals. They give such explanations without being so verbose you want to put your eyes out. So for more discussion on this subject check out the Dako IHC manual 5th edition pg 122 or thereabouts. I hope this helps explain why I feel the buffer steps are essential and really shouldn't be replaced by water rinses. It is a really good question though since we are all trying to save a little money and with how much companies are charging for these buffers, it's a good place to look. IHCworld has some great recipes for making them up yourself and they will save you a fortune. Have a great weekend, Amos Message: 1 Date: Thu, 13 Mar 2014 17:16:52 +0000 From: "Goins, Tresa" Subject: [Histonet] IHC without wash buffer To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" In a continuing effort to limit the volume of reagent used in each step of a manual IHC, I have tried TBS and TBST on slides with barriers (expensive) and without barriers. The results were not stellar with the barrier slides - the reagent still escapes. We dewax with hot detergent (may be a contributing factor) and the Tween 20 in the TBST definitely alters the hydrophobicity of the barrier, an effect that is not reversed with a water wash. Consequently, I have resorted to omitting the buffer wash steps and using distilled water only. The slide surface remains "water repellent" and the added IHC reagents form a pool over the tissue sections. The detection method is a polymer-HRP and there is no increase in background staining in the tissue or on the slide surface. I am assuming that the IHC reagents are prepared in the "optimum" suspension liquid and a buffer is not required. So, I am interested in hearing from anyone why this is a bad idea. Thanks, Tresa From sadey <@t> hotmail.ca Sun Mar 16 07:09:42 2014 From: sadey <@t> hotmail.ca (Sheila Adey) Date: Sun Mar 16 07:09:47 2014 Subject: [Histonet] Interfacing with Meditech Magic Message-ID: Hello Everyone: I am looking for some suggestions. We use Meditech Magic and we are trying to interface with the Leica Cerebro system. We are trying to put the correct info in the .hist section of Meditech so that Cerebro can pull the necessary info for the labels. My unsolved issue at the moment is how to tell cerebro that I need an H&E label and special stain label on the same level. My computer person is saying that the second procedure typed in will over ride the first one ordered. Thanks for any advice. Sheila From johnronan <@t> custompathlabsolutions.com Sun Mar 16 08:51:31 2014 From: johnronan <@t> custompathlabsolutions.com (Custompathlabsolutions) Date: Sun Mar 16 08:51:40 2014 Subject: [Histonet] Interfacing with Meditech Magic In-Reply-To: References: Message-ID: <050A869C-D8BA-47F3-85C8-F0ED3C8F3F2D@custompathlabsolutions.com> Shelia, It's a beautiful Sunday morning so I feel guilty suggesting that you lie but........only to a machine. If you input that the H&E is at level 2 and the next stain is at level 2.1 the new information should not be overridden. When you format the labels you can format the level number as an integer so 2.1 becomes 2. You could also have levels 2, two & II if you want to mislead the computer instead of lie to it. I think as long as one program sees a unique level that your eye can read as the same thing you can get around the problem. John Sent from my iPhone On Mar 16, 2014, at 8:09 AM, Sheila Adey wrote: Hello Everyone: I am looking for some suggestions. We use Meditech Magic and we are trying to interface with the Leica Cerebro system. We are trying to put the correct info in the .hist section of Meditech so that Cerebro can pull the necessary info for the labels. My unsolved issue at the moment is how to tell cerebro that I need an H&E label and special stain label on the same level. My computer person is saying that the second procedure typed in will over ride the first one ordered. Thanks for any advice. Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Sun Mar 16 11:21:27 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Sun Mar 16 11:32:49 2014 Subject: =?utf-8?Q?Re:_[Histonet]_Interfacing_with_Meditech_Magic?= Message-ID: Sheila, I use a tracking / quality system and I believe you should look at the problem from another view. What you did before in a manual way, now has to be reconsidered, developed and constructed with strict logic for counting and tracking all units (specimen, block, slide) throughout your process. Manual systems often mix logic and, in my mind, create complexity that leads to mislabels. Do not try to fool the LIS, work w/ it. When you cut a block and you have H&E slides and special staining (i.e. recut, SS, IHC, ISH) slides, you need to make sure all slides are unique items and will be tracked so. In our system, slides are designated A1-1, A1-2, A1-3. . . and there is communication with the pathologist as to the numbering and what the cut slide contains. We even have protocols built into our system that allows us to cur unstained slides and they can be reconciled back to a special staining slide. I suggest you might want to sit down and ?map out?? (flow chart) your cutting and pathologist ordering process so that you can create the logical tracking process needed to work with your LIS. William DeSalvo BS, HTL(ASCP) Sent from Windows Mail From: Custompathlabsolutions Sent: ?Sunday?, ?March? ?16?, ?2014 ?6?:?52? ?AM To: Sheila Adey Cc: histonet Shelia, It's a beautiful Sunday morning so I feel guilty suggesting that you lie but........only to a machine. If you input that the H&E is at level 2 and the next stain is at level 2.1 the new information should not be overridden. When you format the labels you can format the level number as an integer so 2.1 becomes 2. You could also have levels 2, two & II if you want to mislead the computer instead of lie to it. I think as long as one program sees a unique level that your eye can read as the same thing you can get around the problem. John Sent from my iPhone On Mar 16, 2014, at 8:09 AM, Sheila Adey wrote: Hello Everyone: I am looking for some suggestions. We use Meditech Magic and we are trying to interface with the Leica Cerebro system. We are trying to put the correct info in the .hist section of Meditech so that Cerebro can pull the necessary info for the labels. My unsolved issue at the moment is how to tell cerebro that I need an H&E label and special stain label on the same level. My computer person is saying that the second procedure typed in will over ride the first one ordered. Thanks for any advice. Sheila _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Mar 17 06:35:49 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Mar 17 06:36:01 2014 Subject: [Histonet] Microwave Tissue Processing In-Reply-To: References: , , Message-ID: Aikhan I am happy to share my experience, and very inspirational your concern for the burden on the patient and their families. Your language is just fine ! Do not be concerned. We are on this list to help one another. MW processing fairly common here. Some people love it, and some people are not fully confident in it yet. My first advice would be that the biggest issue to adoption seems to be understanding that it works differently than conventional processing, though the end result accomplishes the same purpose. It works very well if people take the time to learn to use it correctly. It is based more on physics of MW energy and tissue components than just passive reagent diffusion like conventional processors. It is wise to take some time to learn about how this works before starting, though getting past this barrier should be easier for you since you are not accustomed to using a conventional processor. The vendors that I know are Thermo-Shandon, Sakura, Milestone -at least these are the instruments I have personally used ( various models). They all have advantages and disadvantages, it just depends on what your needs are. The Milestone I would say is particularly adopted to very high volumes. The vendors have proprietary technology and some reagents, but most use formalin, but just accelerate the fixation rate with MW energy instead of convection heat and agitation. The big concern is that you are working with much shorter times and so you have to adjust your thinking on this aspect. Also some tissues- liver cores, skins and bloody specimens such as endometrial biopsies will get very dry if you are not careful with the heat and microwave exposure- my theory is that is has to do with the polarity or water content of these tissues. The outer surface becomes almost coagulated and tough, must like over-cooked food in the microwave. Important notes are: That generally you cannot REPROCESS microwave processed tissue. So you have to take the time to do it correctly from the beginning, and all tissue can be sucessfully processed when a suitable program is set up. As you know, you CAN reprocess with conventional, though the results are often not much improved. So carefully consider this as you set up your process ( see below my suggestions for this). The tissue thickness and overall dimensions are even more important with MW than with any type of conventional processing to get uniform and optimal results. On the positive side, large samples( cut thinly), breast tissue and other fatty specimens seem to do exceptionally well, much better than conventional. Graded ethanols are typically used for dehydration as in conventional processing. So, really most of the reagents are the same in the instruments I have used, with the exception that isopropanol is substituted for xylene as a transition and clearing reagent. Even though this seems counterintutitive, it actually seems to work pretty well. If you are accustomed to processing manually, you will be amazed at the tissue quality and speed you can gain by using any type of automated processing- you will be leaps and bounds ahead if you are successful in adopting microwave assisted processing. Advantages signifigantly reduce turn around, faster pateint results- some program are less than 1 hour, really speeding up diagnosis. For some patients and samples this may outweigh almost any disadvantagesthe vendors typically can assit with the intial set up and this takes care of many of the issues I mention under disadvantagescleaner, less exposure to hazardous chemicials Disadvantages Takes more time in the inital design of the programs,and validationYou may need to customize or adjust programs for particular tissues more so than conventional, so a little more technical attention and time is needed, in some labs this may be a barrier I hope that some of this information can be of help to you. Please let me know if I can assist further in any way. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Mon, 17 Mar 2014 14:49:12 +0600 Subject: Re: [Histonet] Microwave Tissue Processing From: aikhanlab@gmail.com To: joelleweaver@hotmail.com Hi Joelle Weaver, >From your posting I learned that you have experience of using Microwave tissue processor for last 8-10 years. I would be grateful if you can help me in light of your experience. We are giving histopathology service in our country, Bangladesh. We have a peculiar situation: Many patients are from rural areas, they come to the city, have to stay till a diagnosis is made, an expensive matter for them bearing all these costs; there's no insurance system here. Presently tissue processing is manual in our lab; around 150 paraffin blocks daily. Needing to produce rapid result under financial constraint we are thinking of microwave processing, but we don't have any experience with these. We know there are other choices as well: conventional carousel (like Shandon Citadel), vacuum single retort (like Sakura tissue-tek) and linear processors (like ATP 700 or ATP 1000). I would be grateful if you can let us know about your experience with MW processors. Some of the questions in my mind about MW tissue processor: 1. What are the reagents required in MW tissue processing. Can we use the same reagent repeatedly in the MW processor, like in conventional processors. If so, how many times we can use the same reagent repeatedly. 2. What are the MW processors available in the market: I have googled and found Milestone, Labpulse. 3. As compared to conventional processor, what are the advantages and disadvantages in your opinion. If you have more to tell us about your experience, please do, I would be happy to learn from you. Please excuse my bad language if something is not clear to you. Regards, aikhan On Fri, Mar 14, 2014 at 6:02 PM, joelle weaver wrote: I have used a couple of vendor's MW processing instruments over the past 8-10 years. So it is used, even if it has not become as commonplace as conventional in every setting or market. It seems to be more favored in high volume settings, for pretty obvious reasons. In teaching and instruction * my opinion * - you should teach them the theory and fundamentals for practice for ALL the possible tissue processing technologies they may encounter, and this is consistent with the approach to practice of the topics on the ASCP exam.They have to know the fundamental basics and then it is easy to expand to more emerging practices and technology. It would be more of a disservice to me if you left anything( either conventional technology or MW out), in your treatment of that topic. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: KGoodkowsky@goodwin.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 14 Mar 2014 11:47:26 +0000 > Subject: [Histonet] Microwave Tissue Processing > > Hello all, > I had an opportunity to demo a microwave tissue processing unit for my students. Is anyone using microwave technology for tissue processing and if so, could you please provide me some information on your experience with this? There are many pros that I can see, including its ease of use and quick processing time which fits well with the student lab schedule. I am wondering, however, what the likelihood will be that students will use this technology once in the field. I don't want to do them a disservice by not using conventional tissue processing methods. The majority of hospitals in the CT/MA area use conventional tissue processors. > > Thank you. > > Sent from my iPad > Kelli Goodkowsky > Director Clinical Education, Histologic Science > Goodwin College > (860) 727-6917 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JThawley <@t> ShoreMemorial.org Mon Mar 17 10:04:21 2014 From: JThawley <@t> ShoreMemorial.org (JThawley@ShoreMemorial.org) Date: Mon Mar 17 10:04:29 2014 Subject: [Histonet] Job Opportunity in NJ In-Reply-To: References: Message-ID: Exciting News! Shore Medical Center is currently accepting applications/resumes for certified Histotechnologists. Two positions open - One is a PRN position and the other is Full-Time (40 hours a week). Either position is responsible for processing, embedding, cutting and staining of tissue slides, accurate quality control, special projects, equipment maintenance and all other duties assigned by the Supervisor. This is your chance to put your skills to work with a company dedicated to being a best place to work. Shore Medical Center has been recognized as a ?Planetree Designated Patient-Centered Hospital?.? This designation acknowledges Shore?s achievement and innovation in the delivery of patient-centered care. Shore is the only hospital in New Jersey and one of only 39 healthcare organizations worldwide to receive the Patient-Centered Designation since the program?s launch in 2007. Shore Medical Centers Values: Put Patients First Respect Others Pursue Excellence Do the Right Thing If these words resonate with you, then explore job opportunities with us. Please contact me via phone and/or email. I look forward to hearing from you! Jennifer Thawley, MHA, HT (ASCP) Histology Supervisor Shore Medical Center (609)653-3940 "Change is the law of life. And those who look only to the past or present are certain to miss the future." John F. Kennedy From cjohnson <@t> nmda.nmsu.edu Mon Mar 17 10:48:05 2014 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Mon Mar 17 10:48:10 2014 Subject: [Histonet] Biossusa antibodies? Message-ID: Hello all, Has anyone had experience with antibodies from Biossusa? I was looking for antibodies and came across this company but I am not familiar with them. Any help is appreciated. Happy Monday, Carole Carole Johnson, HT(ASCP)cm Histopathology Section Supervisor New Mexico Department of Agriculture Veterinary Diagnostic Services Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From fbozkurt <@t> gmail.com Mon Mar 17 12:21:15 2014 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Mon Mar 17 12:21:26 2014 Subject: [Histonet] Biossusa antibodies? In-Reply-To: References: Message-ID: Hello, I haven't use their antibodies yet. They take 20 %fee for returned unopened antibodies. On Mon, Mar 17, 2014 at 5:48 PM, Johnson, Carole wrote: > Hello all, > > Has anyone had experience with antibodies from Biossusa? I was looking for > antibodies and came across this company but I am not familiar with them. > Any help is appreciated. > > Happy Monday, > Carole > > Carole Johnson, HT(ASCP)cm > Histopathology Section Supervisor > New Mexico Department of Agriculture > Veterinary Diagnostic Services > > > > > Confidentiality Notice: New Mexico has a very broad public records law. > Most written communications to or from state employees are public records. > Your e-mail communications may therefore be subject to public disclosure. > This e-mail, including all attachments is for the sole use of the intended > recipients. Any unauthorized review, use, disclosure or distribution is > prohibited unless specifically provided under the New Mexico Inspection of > Public Records Act. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 Asst. Prof. Dr. Mehmet Fatih BOZKURT Manager of Veterinary Diagnostic Laboratory Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16300-16301 vetanaliz@aku.edu.tr From Richard.Cartun <@t> hhchealth.org Mon Mar 17 13:07:49 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Mar 17 13:07:55 2014 Subject: [Histonet] RE: IHC Validation In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F428996@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F428996@BFL323E10.pathmdlabs.local> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E02289E7E@HHCEXCHMB03.hhcsystem.org> Are you changing the antibody dilution? If so, I would run 2-3 positive and negative cases and make sure that the immunoreactivity compares favorably to what you observed before. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, March 11, 2014 5:27 PM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] IHC Validation If I have validated an antibody at a specific incubation time, and then later want to decrease the incubation time, do I have to re-validate?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Erin.Martin <@t> ucsf.edu Mon Mar 17 13:19:42 2014 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Mar 17 13:19:57 2014 Subject: [Histonet] Hemoglobin stain Message-ID: <24B7B291CC88D04AB663958E77A1F59D19CB0D@ex09.net.ucsf.edu> Hi all, I have a pathologist that is looking for a special stain that "highlights blood." Initially she had wanted to use benzidine as referenced in the article below but our safety office let me know that we would need some pretty hefty air scrubbers and respirators to use it safely. Does anyone have a protocol that might give the same results? Thank you, Erin Martin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Am J Dermatopathol. 1995 Aug;17(4):362-7. Benzidine stain for the histochemical detection of hemoglobin in splinter hemorrhage (subungual hematoma) and black heel. Hafner J(1), Haenseler E, Ossent P, Burg G, Panizzon RG. Author information: (1)Department of Dermatology, University Hospital of Zurich, Switzerland. Minor nail trauma may cause bluish discoloration of the nail, while tangential skin trauma on the heel can result in a so-called black heel. To rule out melanoma in such clinical situations, a biopsy is needed to reveal homogeneous eosinophilic masses deposited under the nail plate or within it (transepidermal elimination). Most dermatopathologists attempt to demonstrate the presence of hemoglobin in these eosinophilic masses with Prussian blue stain, which typically remains negative. In our experience, these traumatically induced blood deposits are always situated in avascular spaces, devoid of degrading phagocytes. Consequently, a histochemical stain for these deposits should be directed specifically toward hemoglobin, not hemosiderin. In the dermatopathologic literature, the various techniques to detect hemoglobin deposits in tissue sections are not well-known. We would like to emphasize benzidine stain, a highly selective and efficient method to demonstrate the presence of hemoglobin deposits in histologic sections. To date, benzidine stain has not been utilized to characterize splinter hemorrhage (subungual hematoma). Of concern, the use of benzidine in histopathology laboratories is restricted because this agent is a known carcinogen, while the non-mutagenic derivative, 3,3',5,5'-tetramethylbenzidine, does not stain histologic sections. Patent blue V, a completely different and less specific agent, stains hemoglobin an intense blue-green. Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From tgenade <@t> gmail.com Mon Mar 17 13:29:29 2014 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Mon Mar 17 13:29:43 2014 Subject: [Histonet] methyl-benzoate for clearing Message-ID: Hello, Is anyone using methyl-benzoate for clearing instead of xylene? Can it be used multiple times like xylene (until it begins to go cloudy from the water); and where are you buying it and what grade is normally used? I've found this at Fisher Scientific: http://www.fishersci.com/ecomm/servlet/fsproductdetail?storeId=10652&productId=16067852&catalogId=29104&matchedCatNo=AC126340250||AC126345000||AC126340025||AC126340100&fromSearch=1&searchKey=methyls||methyl||benzoate&highlightProductsItemsFlag=Y&endecaSearchQuery=%23store%3DScientific%23nav%3D0%23rpp%3D25%23offSet%3D0%23keyWord%3Dmethyl%2Bbenzoate%23searchType%3DPROD%23SWKeyList%3D%5B%5D&xrefPartType=From&savings=0.0&xrefEvent=1395080679905_2&searchType=PROD&hasPromo=1 Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From jpiche <@t> wtbyhosp.org Mon Mar 17 14:28:45 2014 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Mon Mar 17 14:28:52 2014 Subject: [Histonet] Frozen section PPE Message-ID: <631955447A364B45B9458D2905635110AC0FB337@WIN08-MBX-02.wtbyhosp.org> Hi Everyone, We were wondering what people are wearing while doing frozen sections? Mask, goggles, lab coat? We'd like to know what everyone else is doing to protect themselves. Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From TGoins <@t> mt.gov Mon Mar 17 14:42:23 2014 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Mar 17 14:42:37 2014 Subject: [Histonet] IHC wash with dH20 / follow-up Message-ID: Thanks for the responses to my question re IHC wash without buffer. It made me examine the question more thoroughly with empirical comparisons. The Dako IHC manual is a good reference. But, the take home message I got after reviewing it didn't alter my view that buffer wash may not be required. Following the initial electrostatic interaction that occurs between antibody and antigen in the milieu of the antibody diluent, the Ab-Ag bond is maintained via Van der Waals and hydrophobic forces. No matter what the slide is rinsed with, I don't think this microenvironment is going to be altered. In our hands, IHC results with twenty antibodies has not been adversly effected by using dH2O in place of wash buffer (TBST in our case) - staining intensity and contrast with the water wash is equivalent to buffer washed slides. I located a reference that supports the use of a water wash (Hallelujah - I'm not crazy) in: TECHNICAL IMMUNOHISTOCHEMISTRY:Achieving Reliability and Reproducibility of Immunostains. Rodney T. Miller, M.D. Director of Immunohistochemistry ProPath Laboratory, Inc. 8267 Elmbrook, Suite 100, Dallas, Texas 75247-4009 Phone (214) 237-1631 FAX (214) 237-1770 www.propathlab.com E mail: rmiller@propathlab.com An excerpt from the reference states: Although previously I never thought of using anything else for rinsing steps, a conversation with another immunohistochemist (Dave Tacha, BioCare, Walnut Creek, CA) led me to try distilled water with Tween 20 (DW/Tween20) rather than PBS for the rinsing steps. When we evaluated the performance of DW/Tween 20 for our rinsing solution between steps, it performed just as well as using PBS with Tween 20, so we now routinely use DW with 0.2% Tween 20 (2 ml Tween 20 in 10 liters of DW) for all of our rinses that previously used buffer. This has saved us substantial amounts of reagent cost as well as reagent preparation time, since preparing DW/Tween 20 is far easier and cheaper than preparing our previous PBS buffer rinsing solutions. OK, detergent is still present, but with polymner-based IHC, background has not been a problem . . . . .yet. At the very lease, the cost of Tween 20 and distilled water is a whole lot cheaper than Tween 20 and buffer. Happy validating! Tresa Tresa Goins Histopathology Section Montana Veterinary Diagnostic Lab Bozeman, MT 59715 406-994-6353 From latecor <@t> montevideo.com.uy Mon Mar 17 16:14:52 2014 From: latecor <@t> montevideo.com.uy (C.D.G.) Date: Mon Mar 17 15:12:55 2014 Subject: [Histonet] methyl-benzoate for clearing In-Reply-To: References: Message-ID: <201403171814520968.00127528@smtp.montevideo.com.uy> You can use it instead of xylene only as clearing agent before paraffin baths; take note that it is much dificult to eliminate from paraffin stations,so you will have to prolong the time spending at this point. It isn't reccommended for clearing slides, you have many alternatives at this point. Carlos. tgenade@gmail.com *********** REPLY SEPARATOR *********** On 17/03/2014 at 01:29 p.m. Tyrone Genade wrote: >Hello, > >Is anyone using methyl-benzoate for clearing instead of xylene? Can it be >used multiple times like xylene (until it begins to go cloudy from the >water); and where are you buying it and what grade is normally used? > >I've found this at Fisher Scientific: >http://www.fishersci.com/ecomm/servlet/fsproductdetail?storeId=10652&productId=16067852&catalogId=29104&matchedCatNo=AC126340250||AC126345000||AC126340025||AC126340100&fromSearch=1&searchKey=methyls||methyl||benzoate&highlightProductsItemsFlag=Y&endecaSearchQuery=%23store%3DScientific%23nav%3D0%23rpp%3D25%23offSet%3D0%23keyWord%3Dmethyl%2Bbenzoate%23searchType%3DPROD%23SWKeyList%3D%5B%5D&xrefPartType=From&savings=0.0&xrefEvent=1395080679905_2&searchType=PROD&hasPromo=1 > >Thanks >-- >Tyrone Genade >http://tgenade.freeshell.org >email: tgenade@gmail.com > >******************************************************************************** >Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. >To find out how to receive this FREE gift visit http://www.alpha.org. >_______________________________________________ >Histonet mailing list > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BGapinski <@t> pathgroup.com Mon Mar 17 16:19:40 2014 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Mon Mar 17 16:19:47 2014 Subject: [Histonet] What is a level? Message-ID: What is a level? Levels, deepers, we all call it something different, but what exactly is a level? I know it is all relative. One would never cut into a prostate needle biopsy the same way they'd cut into a skin. But if we suppose are tissue sample is 3mm thick (don't laugh). How deeply would you cut when the pathologist asks for a level? I guess I'm talking to those of us with automated microtomes where we can set the trim to 10, 20, 30 microns. I started on a manual microtome where it's impossible to gauge this altogether. So let's say I have a nicely processed ellipse of skin grossed 3mm thick. The pathologist asks me for a level. If we assume I gave them a full face on the first slide, how much deeper should I go to get the level? 60-80 microns? Deeper? Less? Your thoughts please, Respectfully, Bruce Gapinski HT (ASCP) ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From joelleweaver <@t> hotmail.com Mon Mar 17 16:31:45 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Mar 17 16:31:50 2014 Subject: [Histonet] Frozen section PPE In-Reply-To: <631955447A364B45B9458D2905635110AC0FB337@WIN08-MBX-02.wtbyhosp.org> References: <631955447A364B45B9458D2905635110AC0FB337@WIN08-MBX-02.wtbyhosp.org> Message-ID: nitrile gloves, maybe gown or apron, sometimes eye protection, mask if suspected TB. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jpiche@wtbyhosp.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 17 Mar 2014 19:28:45 +0000 > Subject: [Histonet] Frozen section PPE > > Hi Everyone, > > We were wondering what people are wearing while doing frozen sections? Mask, goggles, lab coat? We'd like to know what everyone else is doing to protect themselves. > > Thank you, > > Jessica Piche, HT(ASCP) > Waterbury Hospital > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Mon Mar 17 16:58:11 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon Mar 17 16:58:15 2014 Subject: [Histonet] What is a level? In-Reply-To: References: Message-ID: You should ask the pathologist what their definition of a level. My dermatopathologists want 40 microns (10 rotations of the microtome wheel, cutting at 4 microns) for a level. This is the typical size of most organells they are looking at. Again, the level depth should be discussed and agreed upon by the pathologist and microtomist. This takes the guess work out of the process. once you have a specific description, make sure this is added to your SOP. William DeSalvo, BS HTL(ASCP) > From: BGapinski@pathgroup.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 17 Mar 2014 21:19:40 +0000 > Subject: [Histonet] What is a level? > > What is a level? > Levels, deepers, we all call it something different, but what exactly is a level? I know it is all relative. One would never cut into a prostate needle biopsy the same way they'd cut into a skin. > But if we suppose are tissue sample is 3mm thick (don't laugh). How deeply would you cut when the pathologist asks for a level? I guess I'm talking to those of us with automated microtomes where we can set the trim to 10, 20, 30 microns. I started on a manual microtome where it's impossible to gauge this altogether. > So let's say I have a nicely processed ellipse of skin grossed 3mm thick. The pathologist asks me for a level. If we assume I gave them a full face on the first slide, how much deeper should I go to get the level? 60-80 microns? Deeper? Less? > Your thoughts please, > > > Respectfully, > Bruce Gapinski HT (ASCP) > > > ________________________________ > > Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Mon Mar 17 22:22:35 2014 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Mar 17 22:22:58 2014 Subject: [Histonet] DAB for hemoglobin? Message-ID: <24B7B291CC88D04AB663958E77A1F59D19CBF6@ex09.net.ucsf.edu> Good morning all, I posted yesterday regarding the use of benzidine for the detection of hemoglobin. My pathologist believes that standard DAB can be used instead of benzidine solution but I can't find any references for using DAB as a hemoglobin stain. Do any of you fine histo folks do this? Thank you! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From Susan.Walzer <@t> HCAHealthcare.com Tue Mar 18 01:51:56 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Mar 18 01:52:04 2014 Subject: [Histonet] What is a level? In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FB5806495@FWDCWPMSGCMS09.hca.corpad.net> 10 turns of the wheel is fine for most tissue however we often get FNA's that are so tiny that several turns of the wheel and you will be completely through it. It is often a judgment call by the tech. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, March 17, 2014 5:58 PM To: Bruce Gapinski; histonet Subject: RE: [Histonet] What is a level? You should ask the pathologist what their definition of a level. My dermatopathologists want 40 microns (10 rotations of the microtome wheel, cutting at 4 microns) for a level. This is the typical size of most organells they are looking at. Again, the level depth should be discussed and agreed upon by the pathologist and microtomist. This takes the guess work out of the process. once you have a specific description, make sure this is added to your SOP. William DeSalvo, BS HTL(ASCP) > From: BGapinski@pathgroup.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 17 Mar 2014 21:19:40 +0000 > Subject: [Histonet] What is a level? > > What is a level? > Levels, deepers, we all call it something different, but what exactly is a level? I know it is all relative. One would never cut into a prostate needle biopsy the same way they'd cut into a skin. > But if we suppose are tissue sample is 3mm thick (don't laugh). How deeply would you cut when the pathologist asks for a level? I guess I'm talking to those of us with automated microtomes where we can set the trim to 10, 20, 30 microns. I started on a manual microtome where it's impossible to gauge this altogether. > So let's say I have a nicely processed ellipse of skin grossed 3mm thick. The pathologist asks me for a level. If we assume I gave them a full face on the first slide, how much deeper should I go to get the level? 60-80 microns? Deeper? Less? > Your thoughts please, > > > Respectfully, > Bruce Gapinski HT (ASCP) > > > ________________________________ > > Important Notice: This e-mail is intended for the use of the person to > whom it is addressed and may contain information that is privileged > and confidential. If you are not the intended recipient, any > disclosure, copying, distribution, or use of the contents of this > message is strictly prohibited. If you have received this e-mail in > error, please destroy this message and contact the Security Officer at > PathGroup, Inc immediately at 615-562-9255. Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> skm.org.pk Tue Mar 18 04:58:36 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Tue Mar 18 04:58:46 2014 Subject: [Histonet] fixed fecal smears for CAP parasitology survey 2014 p3 Message-ID: Hi All, We have received two fixed fecal smears from microbiology section ( CAP parasitology survey 2014 p3,)for trichrome or iron-Hematoxylin staining procedures. Would I adopted any kind of trichrome or iron Hematoxylin or some specific ? Thank, Muhammad Tahseen SKMCH&RC Lahore From tbraud <@t> holyredeemer.com Tue Mar 18 12:50:01 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Mar 18 12:50:07 2014 Subject: [Histonet] RE: Frozen section PPE In-Reply-To: <20140318162201.B32691E806D@trendmess-svr.holyredeemer.local> References: <20140318162201.B32691E806D@trendmess-svr.holyredeemer.local> Message-ID: Our 2 cents. For performing frozen sections, we require gown and gloves. If there is a possibility of a infectious granuloma then we add goggles and a mask. Also, we never allow spray coolant in the cryostat as it has the probability of aerosolizing any nasty bugs lurking in the cryochamber. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 5 Date: Mon, 17 Mar 2014 19:28:45 +0000 From: "Piche, Jessica" Subject: [Histonet] Frozen section PPE Hi Everyone, We were wondering what people are wearing while doing frozen sections? Mask, goggles, lab coat? We'd like to know what everyone else is doing to protect themselves. Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital *************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From tbraud <@t> holyredeemer.com Tue Mar 18 13:03:21 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Mar 18 13:03:25 2014 Subject: [Histonet] RE: what is a level In-Reply-To: <20140318162201.B32691E806D@trendmess-svr.holyredeemer.local> References: <20140318162201.B32691E806D@trendmess-svr.holyredeemer.local> Message-ID: Our 2 cents: What is a level? A level is how you've defined level in your SOP. In this lab, we use the words level and recut interchangeably. We define a level as 40 microns deeper than the previous section. If the pathologist wants to see every section of tissue cut without skipping any tissue, then they order "sequential" sections. If we get orders to go into a block a second time, call it recut or level, then the 1st slide is always the first full face section obtained. If the tech senses that the orders will exhaust the tissue in the block, then they communicate that to the pathologist before cutting. If the pathologist orders the block to be cut through, we will ask them how many slides they want and space them accordingly. Its not really that complicated and it works for us. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 *********************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From one_angel_secret <@t> yahoo.com Tue Mar 18 13:15:23 2014 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Mar 18 13:15:37 2014 Subject: [Histonet] IHC wash with dH20 / follow-up In-Reply-To: References: Message-ID: <45C699F9-128D-4315-9A7F-81A7E97FC622@yahoo.com> Remember you are doing them by hand do you can manually place reagent where you want it. Buffer would be necessary on a machine if for nothing else other than as a surfactant to insure all corresponding reagents spread evenly on the slide. I just wanted to add this note in case some did not catch that this conclusion was for doing by hand. Although my opinion is that even by hand it is best practice to use a buffer due to previous comments about electrostatic benefits of using a surfactant. My two cents :) Kim On Mar 17, 2014, at 3:42 PM, "Goins, Tresa" wrote: > Thanks for the responses to my question re IHC wash without buffer. It made me examine the question more thoroughly with empirical comparisons. > > The Dako IHC manual is a good reference. But, the take home message I got after reviewing it didn't alter my view that buffer wash may not be required. Following the initial electrostatic interaction that occurs between antibody and antigen in the milieu of the antibody diluent, the Ab-Ag bond is maintained via Van der Waals and hydrophobic forces. No matter what the slide is rinsed with, I don't think this microenvironment is going to be altered. > > In our hands, IHC results with twenty antibodies has not been adversly effected by using dH2O in place of wash buffer (TBST in our case) - staining intensity and contrast with the water wash is equivalent to buffer washed slides. > > I located a reference that supports the use of a water wash (Hallelujah - I'm not crazy) in: > > TECHNICAL IMMUNOHISTOCHEMISTRY:Achieving Reliability and Reproducibility of Immunostains. > > Rodney T. Miller, M.D. > Director of Immunohistochemistry > ProPath Laboratory, Inc. > 8267 Elmbrook, Suite 100, Dallas, Texas 75247-4009 > Phone (214) 237-1631 FAX (214) 237-1770 > www.propathlab.com E mail: rmiller@propathlab.com > > An excerpt from the reference states: > > Although previously I never thought of using anything else for rinsing steps, a conversation with another immunohistochemist (Dave Tacha, BioCare, Walnut Creek, CA) led me to try distilled water with Tween 20 (DW/Tween20) rather than PBS for the rinsing steps. When we evaluated the performance of DW/Tween 20 for our rinsing solution between steps, it performed just as well as using PBS with Tween 20, so we now routinely use DW with 0.2% Tween 20 (2 ml Tween 20 in 10 liters of DW) for all of our rinses that previously used buffer. This has saved us substantial amounts of reagent cost as well as reagent preparation time, since preparing DW/Tween 20 is far easier and cheaper than preparing our previous PBS buffer rinsing solutions. > > OK, detergent is still present, but with polymner-based IHC, background has not been a problem . . . . .yet. > At the very lease, the cost of Tween 20 and distilled water is a whole lot cheaper than Tween 20 and buffer. > > Happy validating! > > Tresa > > > > > Tresa Goins > Histopathology Section > Montana Veterinary Diagnostic Lab > Bozeman, MT 59715 > 406-994-6353 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Mar 18 13:31:27 2014 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Tue Mar 18 13:31:35 2014 Subject: [Histonet] RE: what is a level In-Reply-To: References: <20140318162201.B32691E806D@trendmess-svr.holyredeemer.local> Message-ID: <005601cf42d8$46b3c770$d41b5650$@ihctech.net> It can be confusing because people use different words to describe, we call picking up every section "serial sectioning" and the distance between sections for levels is determined by the requestor in our case, so they might ask for six more levels to be cut with 50 microns thrown out between each level. We leave that up to our customers to choose because they pay by the slide. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, March 18, 2014 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: what is a level Our 2 cents: What is a level? A level is how you've defined level in your SOP. In this lab, we use the words level and recut interchangeably. We define a level as 40 microns deeper than the previous section. If the pathologist wants to see every section of tissue cut without skipping any tissue, then they order "sequential" sections. If we get orders to go into a block a second time, call it recut or level, then the 1st slide is always the first full face section obtained. If the tech senses that the orders will exhaust the tissue in the block, then they communicate that to the pathologist before cutting. If the pathologist orders the block to be cut through, we will ask them how many slides they want and space them accordingly. Its not really that complicated and it works for us. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 *********************************** ---------------------------------------------------------------------------- ----- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susanhay59 <@t> gmail.com Tue Mar 18 13:31:44 2014 From: susanhay59 <@t> gmail.com (S hay) Date: Tue Mar 18 13:31:48 2014 Subject: [Histonet] Thrombomodulin positive controls Message-ID: Hi histoland.....anyone have any tissue control blocks positive for thrombomodulin that u can part with. We would appreciate it tremendously. Susan Memorial Hospital Belleville. Illinois. From TGoins <@t> mt.gov Tue Mar 18 14:46:58 2014 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Tue Mar 18 14:47:08 2014 Subject: [Histonet] IHC wash with dH20 / follow-up In-Reply-To: <45C699F9-128D-4315-9A7F-81A7E97FC622@yahoo.com> References: <45C699F9-128D-4315-9A7F-81A7E97FC622@yahoo.com> Message-ID: I may be wrong but I think that the: Electrostatic benefit is imparted by the salts in the buffer The surfactant benefit is imparted by the detergent -----Original Message----- From: Kim Donadio [mailto:one_angel_secret@yahoo.com] Sent: Tuesday, March 18, 2014 12:15 PM To: Goins, Tresa Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC wash with dH20 / follow-up Remember you are doing them by hand do you can manually place reagent where you want it. Buffer would be necessary on a machine if for nothing else other than as a surfactant to insure all corresponding reagents spread evenly on the slide. I just wanted to add this note in case some did not catch that this conclusion was for doing by hand. Although my opinion is that even by hand it is best practice to use a buffer due to previous comments about electrostatic benefits of using a surfactant. My two cents :) Kim On Mar 17, 2014, at 3:42 PM, "Goins, Tresa" wrote: > Thanks for the responses to my question re IHC wash without buffer. It made me examine the question more thoroughly with empirical comparisons. > > The Dako IHC manual is a good reference. But, the take home message I got after reviewing it didn't alter my view that buffer wash may not be required. Following the initial electrostatic interaction that occurs between antibody and antigen in the milieu of the antibody diluent, the Ab-Ag bond is maintained via Van der Waals and hydrophobic forces. No matter what the slide is rinsed with, I don't think this microenvironment is going to be altered. > > In our hands, IHC results with twenty antibodies has not been adversly effected by using dH2O in place of wash buffer (TBST in our case) - staining intensity and contrast with the water wash is equivalent to buffer washed slides. > > I located a reference that supports the use of a water wash (Hallelujah - I'm not crazy) in: > > TECHNICAL IMMUNOHISTOCHEMISTRY:Achieving Reliability and Reproducibility of Immunostains. > > Rodney T. Miller, M.D. > Director of Immunohistochemistry > ProPath Laboratory, Inc. > 8267 Elmbrook, Suite 100, Dallas, Texas 75247-4009 > Phone (214) 237-1631 FAX (214) 237-1770 > www.propathlab.com E mail: rmiller@propathlab.com > > An excerpt from the reference states: > > Although previously I never thought of using anything else for rinsing steps, a conversation with another immunohistochemist (Dave Tacha, BioCare, Walnut Creek, CA) led me to try distilled water with Tween 20 (DW/Tween20) rather than PBS for the rinsing steps. When we evaluated the performance of DW/Tween 20 for our rinsing solution between steps, it performed just as well as using PBS with Tween 20, so we now routinely use DW with 0.2% Tween 20 (2 ml Tween 20 in 10 liters of DW) for all of our rinses that previously used buffer. This has saved us substantial amounts of reagent cost as well as reagent preparation time, since preparing DW/Tween 20 is far easier and cheaper than preparing our previous PBS buffer rinsing solutions. > > OK, detergent is still present, but with polymner-based IHC, background has not been a problem . . . . .yet. > At the very lease, the cost of Tween 20 and distilled water is a whole lot cheaper than Tween 20 and buffer. > > Happy validating! > > Tresa > > > > > Tresa Goins > Histopathology Section > Montana Veterinary Diagnostic Lab > Bozeman, MT 59715 > 406-994-6353 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Mar 18 15:36:23 2014 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Mar 18 15:36:35 2014 Subject: [Histonet] IHC wash with dH20 / follow-up In-Reply-To: References: <45C699F9-128D-4315-9A7F-81A7E97FC622@yahoo.com> Message-ID: <40B4B5D1-8FB3-4B0F-BB4D-D52332909A70@yahoo.com> Buffer in of itself acts as a surfactant on the tissue sections a wash buffer such as a tris(TBS)has salts in it. Saline is a salt solution as you probably know. I'm just giving my opinion as to the benefits of using a buffer but more importantly for anyone out there using a instrument for IHC to not use a wash buffer would have a nightmare. Manually you might be able to get away with it for some antibodies. It's just not a recommendation I personally would make. Hope this helps. Thanks Kim D Sent from my iPhone On Mar 18, 2014, at 3:46 PM, "Goins, Tresa" wrote: > I may be wrong but I think that the: > Electrostatic benefit is imparted by the salts in the buffer > The surfactant benefit is imparted by the detergent > > > > -----Original Message----- > From: Kim Donadio [mailto:one_angel_secret@yahoo.com] > Sent: Tuesday, March 18, 2014 12:15 PM > To: Goins, Tresa > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC wash with dH20 / follow-up > > Remember you are doing them by hand do you can manually place reagent where you want it. Buffer would be necessary on a machine if for nothing else other than as a surfactant to insure all corresponding reagents spread evenly on the slide. > > I just wanted to add this note in case some did not catch that this conclusion was for doing by hand. Although my opinion is that even by hand it is best practice to use a buffer due to previous comments about electrostatic benefits of using a surfactant. > > My two cents :) > > Kim > > On Mar 17, 2014, at 3:42 PM, "Goins, Tresa" wrote: > >> Thanks for the responses to my question re IHC wash without buffer. It made me examine the question more thoroughly with empirical comparisons. >> >> The Dako IHC manual is a good reference. But, the take home message I got after reviewing it didn't alter my view that buffer wash may not be required. Following the initial electrostatic interaction that occurs between antibody and antigen in the milieu of the antibody diluent, the Ab-Ag bond is maintained via Van der Waals and hydrophobic forces. No matter what the slide is rinsed with, I don't think this microenvironment is going to be altered. >> >> In our hands, IHC results with twenty antibodies has not been adversly effected by using dH2O in place of wash buffer (TBST in our case) - staining intensity and contrast with the water wash is equivalent to buffer washed slides. >> >> I located a reference that supports the use of a water wash (Hallelujah - I'm not crazy) in: >> >> TECHNICAL IMMUNOHISTOCHEMISTRY:Achieving Reliability and Reproducibility of Immunostains. >> >> Rodney T. Miller, M.D. >> Director of Immunohistochemistry >> ProPath Laboratory, Inc. >> 8267 Elmbrook, Suite 100, Dallas, Texas 75247-4009 >> Phone (214) 237-1631 FAX (214) 237-1770 >> www.propathlab.com E mail: rmiller@propathlab.com >> >> An excerpt from the reference states: >> >> Although previously I never thought of using anything else for rinsing steps, a conversation with another immunohistochemist (Dave Tacha, BioCare, Walnut Creek, CA) led me to try distilled water with Tween 20 (DW/Tween20) rather than PBS for the rinsing steps. When we evaluated the performance of DW/Tween 20 for our rinsing solution between steps, it performed just as well as using PBS with Tween 20, so we now routinely use DW with 0.2% Tween 20 (2 ml Tween 20 in 10 liters of DW) for all of our rinses that previously used buffer. This has saved us substantial amounts of reagent cost as well as reagent preparation time, since preparing DW/Tween 20 is far easier and cheaper than preparing our previous PBS buffer rinsing solutions. >> >> OK, detergent is still present, but with polymner-based IHC, background has not been a problem . . . . .yet. >> At the very lease, the cost of Tween 20 and distilled water is a whole lot cheaper than Tween 20 and buffer. >> >> Happy validating! >> >> Tresa >> >> >> >> >> Tresa Goins >> Histopathology Section >> Montana Veterinary Diagnostic Lab >> Bozeman, MT 59715 >> 406-994-6353 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Vickroy.Jim <@t> mhsil.com Tue Mar 18 16:20:45 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Mar 18 16:20:59 2014 Subject: [Histonet] ER and PR Controls Message-ID: Lately I have been reviewing our controls on many of our IHC stains. As I was reading a CAP article it caused me to question our ER and PR controls currently being used. Our control has two pieces of tissue in it. One is a breast tumor positive for ER and PR and the other is a breast tumor negative for ER and PR. The article suggested that we should also use a "tumor that shows low or intermediate levels of expression to more sensitively assess subtle drift in assay performance." Of course our current control blocks show staining of normal epithelial cells which also helps us assess sensitivity. My question is what are others using now for controls for ER and PR and how have CAP inspection members been handling this lately. I would appreciate your procedure or opinion. Thanks PS. Our Her2 control blocks also have a tumor that is 3+ and a tumor that is 0-1+. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From vperez <@t> pathreflab.com Tue Mar 18 17:28:54 2014 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Tue Mar 18 17:28:59 2014 Subject: [Histonet] ERG and PTEN antibodies Message-ID: Does anyone out there have any control slides or blocks they can spare for ERG and PTEN. Our docs want to bring in this antibody, but we don't send out our prostate biopsies for these tests so we don't have any for sure positives for these antibodies. Thanks!!! Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com From Tony.Reilly <@t> health.qld.gov.au Tue Mar 18 18:42:52 2014 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Tue Mar 18 18:45:15 2014 Subject: [Histonet] RE: fixed fecal smears for CAP parasitology survey 2014 p3 In-Reply-To: References: Message-ID: Hi Muhammad I have done many of these over the years. You want Gomori's one step Trichrome for protozoa etc or Heidenhain's Iron Haematoxylin. They should be easy to google. If you have any problems let me know and I can send some protocols. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _________________________________________________ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor Sent: Tuesday, 18 March 2014 7:59 PM To: Tony Reilly; histonet@lists.utsouthwestern.edu Subject: [Histonet] fixed fecal smears for CAP parasitology survey 2014 p3 Hi All, We have received two fixed fecal smears from microbiology section ( CAP parasitology survey 2014 p3,)for trichrome or iron-Hematoxylin staining procedures. Would I adopted any kind of trichrome or iron Hematoxylin or some specific ? Thank, Muhammad Tahseen SKMCH&RC Lahore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From murphyv <@t> karmanos.org Wed Mar 19 08:15:52 2014 From: murphyv <@t> karmanos.org (Murphy, Valerie) Date: Wed Mar 19 08:16:01 2014 Subject: [Histonet] Processing frozen brain samples Message-ID: Hi, I am new to Histonet. Here is my question. We have brain samples that were fixed in 4% paraformaldehyde then placed in 30% sucrose (cryoprotectant) prior to freezing on dry ice. These samples have been stored at -80 for a couple of years. We want to thaw these samples then process them to make paraffin embedded blocks. What is the best way to remove the sucrose prior to processing? Should we just rinse with PBS? Thank you, Valerie Valerie Ratliff B.Sc HTL(ASCP) Research Assistant Department of Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: murphyv@karmanos.org Better treatments. Better outcomes. ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. From c.tague <@t> Pathologyarts.com Wed Mar 19 09:10:10 2014 From: c.tague <@t> Pathologyarts.com (Curt) Date: Wed Mar 19 09:10:18 2014 Subject: [Histonet] Thrombomodulin positive controls In-Reply-To: References: Message-ID: <9C8F910F72893643B3C3793C3D67132B0146D874@PATHOLOGYSERVER.pathologyarts.local> We have plenty, we just use placenta, I can send you a block or two if still needed. What's everyone else using? Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of S hay Sent: Tuesday, March 18, 2014 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thrombomodulin positive controls Hi histoland.....anyone have any tissue control blocks positive for thrombomodulin that u can part with. We would appreciate it tremendously. Susan Memorial Hospital Belleville. Illinois. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Wed Mar 19 09:10:35 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Mar 19 09:14:10 2014 Subject: [Histonet] Histology Laboratory Staffing Message-ID: <010b01cf437c$ff51a460$fdf4ed20$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day. I wanted to put up a quick post because I have been talking to some managers who have told me they have been having trouble finding "the right techs" for the positions they have open in their labs. I know it is tricky at best to find that perfect combination of skills, certification and chemistry with the rest of the staff. After all it is what I do all day long. I post jobs here on the histonet pretty regularly and on the flipside I just wanted to let the hiring managers know that if you need assistance with any open positions I am here to help. Also due to recent recruiting and networking efforts I am working with several top notch histotechs and a really special histology supervisor and would love to share them with you if you have a need. So if you have a need now or in the near future please let me know. As a matter of fact if you know of anyone else who may have a need please feel free to pass my posting along to them as well. For the past 12 years I have specialized exclusively in the permanent placement of histology professionals nationwide and pride myself on finding you the candidates you could not find on your own. Thanks for taking the time to read my post. Have a great day!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2014 is Histotechnology Professionals Day. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From GauchV <@t> mail.amc.edu Wed Mar 19 09:25:15 2014 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Wed Mar 19 09:25:22 2014 Subject: [Histonet] Question for Labs performing Electron Microscopy Message-ID: Hi everyone, I have been asked to post the following question regarding EM... If you are providing EM services in your laboratory, who is performing the EM, what is their job title and what are their job duties ( do they process, embed, cut,stain,etc. or just certain portions ). Any information would be greatly appreciated... Please feel free to e-mail me directly or through the Histonet ... Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From rjbuesa <@t> yahoo.com Wed Mar 19 09:30:59 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 19 09:33:53 2014 Subject: [Histonet] Question for Labs performing Electron Microscopy In-Reply-To: Message-ID: <1395239459.49206.YahooMailBasic@web120405.mail.ne1.yahoo.com> We used to do TEM at our lab. The work volume was not high and one of histotechnologists was trained to do ALL tasks (fixation ? printing the photos of the areas selected by the pathologist). When not doing TEM tasks, he took care of any of any of all HTL tasks for which he was also trained. Ren? J. -------------------------------------------- On Wed, 3/19/14, Gauch, Vicki wrote: Subject: [Histonet] Question for Labs performing Electron Microscopy To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 19, 2014, 10:25 AM Hi everyone, I have been asked to post the following question regarding EM... If you are providing EM services in your laboratory, who is performing the EM, what is their job title and what are their job duties ( do they process, embed, cut,stain,etc. or just certain portions ). Any information would be greatly appreciated...? Please feel free to e-mail me directly or through the Histonet ... Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Wed Mar 19 10:04:27 2014 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Wed Mar 19 10:04:32 2014 Subject: [Histonet] Question for Labs performing Electron Microscopy In-Reply-To: <1395239459.49206.YahooMailBasic@web120405.mail.ne1.yahoo.com> References: <1395239459.49206.YahooMailBasic@web120405.mail.ne1.yahoo.com> Message-ID: It's the same for me here. But then again, we're pure research. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 > We used to do TEM at our lab. The work volume was not high and one of > histotechnologists was trained to do ALL tasks (fixation ??? printing the > photos of the areas selected by the pathologist). > When not doing TEM tasks, he took care of any of any of all HTL tasks for > which he was also trained. > Ren?? J. > > -------------------------------------------- > On Wed, 3/19/14, Gauch, Vicki wrote: > > Subject: [Histonet] Question for Labs performing Electron Microscopy > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 19, 2014, 10:25 AM > > Hi everyone, > I have been asked to post the following question regarding > EM... > If you are providing EM services in your laboratory, who is > performing the EM, what is their job title and what are > their job duties ( do they process, embed, cut,stain,etc. or > just certain portions ). > Any information would be greatly appreciated...?? Please > feel free to e-mail me directly or through the Histonet ... > > Thanks, > Vicki Gauch > AMCH > Albany, NY > > > ----------------------------------------- > CONFIDENTIALITY NOTICE: This email and any attachments may > contain confidential information that is protected by law > and is for the sole use of the individuals or entities to > which it is addressed. If you are not the intended > recipient, please notify the sender by replying to this > email and destroying all copies of the communication and > attachments. Further use, disclosure, copying, distribution > of, or reliance upon the contents of this email and > attachments is strictly prohibited. To contact Albany > Medical Center, or for a copy of our privacy practices, > please visit us on the Internet at www.amc.edu. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > FAX (732) 445-6905 From Mark.Elliott <@t> hli.ubc.ca Wed Mar 19 12:53:17 2014 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Wed Mar 19 12:54:00 2014 Subject: [Histonet] Re: Histonet Digest, Vol 124, Issue 20 In-Reply-To: References: Message-ID: <5329771D020000D6000B2C7D@mail.hli.ubc.ca> We have one tech who does all of the steps in EM processing, staining etc., but we are a research lab. Mark Elliott Centre for Heart Lung Innovation Vancouver, BC Date: Wed, 19 Mar 2014 14:25:15 +0000 From: "Gauch, Vicki" Subject: [Histonet] Question for Labs performing Electron Microscopy Hi everyone, I have been asked to post the following question regarding EM... If you are providing EM services in your laboratory, who is performing the EM, what is their job title and what are their job duties ( do they process, embed, cut,stain,etc. or just certain portions ). Any information would be greatly appreciated... Please feel free to e-mail me directly or through the Histonet ... Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. ------------------------------ Message: 15 Date: Wed, 19 Mar 2014 07:30:59 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Question for Labs performing Electron Microscopy To: "histonet@lists.utsouthwestern.edu" ,VickiGauch Message-ID: <1395239459.49206.YahooMailBasic@web120405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=utf-8 We used to do TEM at our lab. The work volume was not high and one of histotechnologists was trained to do ALL tasks (fixation ??? printing the photos of the areas selected by the pathologist). When not doing TEM tasks, he took care of any of any of all HTL tasks for which he was also trained. Ren?? J. -------------------------------------------- On Wed, 3/19/14, Gauch, Vicki wrote: Subject: [Histonet] Question for Labs performing Electron Microscopy To: "histonet@lists.utsouthwestern.edu" Date: Wednesday, March 19, 2014, 10:25 AM Hi everyone, I have been asked to post the following question regarding EM... If you are providing EM services in your laboratory, who is performing the EM, what is their job title and what are their job duties ( do they process, embed, cut,stain,etc. or just certain portions ). Any information would be greatly appreciated...? Please feel free to e-mail me directly or through the Histonet ... Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 19 Mar 2014 11:04:27 -0400 (EDT) From: kgrobert@rci.rutgers.edu Subject: Re: [Histonet] Question for Labs performing Electron Microscopy To: "histonet" Message-ID: Content-Type: text/plain;charset=iso-8859-1 It's the same for me here. But then again, we're pure research. Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 > We used to do TEM at our lab. The work volume was not high and one of > histotechnologists was trained to do ALL tasks (fixation ??? printing the > photos of the areas selected by the pathologist). > When not doing TEM tasks, he took care of any of any of all HTL tasks for > which he was also trained. > Ren?? J. > > -------------------------------------------- > On Wed, 3/19/14, Gauch, Vicki wrote: > > Subject: [Histonet] Question for Labs performing Electron Microscopy > To: "histonet@lists.utsouthwestern.edu" > > Date: Wednesday, March 19, 2014, 10:25 AM > > Hi everyone, > I have been asked to post the following question regarding > EM... > If you are providing EM services in your laboratory, who is > performing the EM, what is their job title and what are > their job duties ( do they process, embed, cut,stain,etc. or > just certain portions ). > Any information would be greatly appreciated...? Please > feel free to e-mail me directly or through the Histonet ... > > Thanks, > Vicki Gauch > AMCH > Albany, NY > > > ----------------------------------------- > CONFIDENTIALITY NOTICE: This email and any attachments may > contain confidential information that is protected by law > and is for the sole use of the individuals or entities to > which it is addressed. If you are not the intended > recipient, please notify the sender by replying to this > email and destroying all copies of the communication and > attachments. Further use, disclosure, copying, distribution > of, or reliance upon the contents of this email and > attachments is strictly prohibited. To contact Albany > Medical Center, or for a copy of our privacy practices, > please visit us on the Internet at www.amc.edu. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > FAX (732) 445-6905 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 124, Issue 20 ***************************************** From TJohnson <@t> gnf.org Wed Mar 19 13:56:15 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Mar 19 13:56:22 2014 Subject: [Histonet] Re: Processing frozen brain samples Message-ID: <9F3CFEE76E51B64991C7485270890B4049848C9F@EX5.lj.gnf.org> Hi Valerie, Why would you need to remove the sucrose? I would place the frozen blocks in room temperature fixative or buffer with one or two changes, especially if you have OCT that needs also to thaw. Why fixative? You may want to consider adding additional fixation if the tissue was minimally fixed in the 4% PFA prior to freezing; the best paraffin processing starts with well fixed samples prior to exposure to alcohol, xylene and heated paraffin. Inadequate fixation time will result in having your sample complete its fixation in the dehydration alcohols. Not necessarily a bad thing if all your work is based on samples partially fixed in aldehyde, and partially fixed in alcohol. Much harder to standardize that IMHO. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From Herrick.James <@t> mayo.edu Wed Mar 19 14:52:52 2014 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Wed Mar 19 14:52:57 2014 Subject: [Histonet] Question on Fluorochromes for Histomorphometry Message-ID: <3dfcdc$fujujs@ironport9.mayo.edu> Dear Histonet Colleagues, I hope everyone is doing well-- We are going to be using a goat mandible model (embedded in MMA) for a project and will need to administer tetracycline and/or calcein (others if suggested) for histomorphometric analysis. Would anyone be kind enough to share their knowledge on which fluorochromes are optimal, dosing requirements (volume, duration and site of injection), etc. We will possibly have three time points. The first at 1 month, the second at 3 months and the third at 6 months. Any help on this would be greatly appreciated!! Have a great day!! Jim From Bruce_Palmatier <@t> vwr.com Wed Mar 19 15:00:38 2014 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Wed Mar 19 15:01:03 2014 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 03/21/2014) Message-ID: I am out of the office until 03/21/2014. I will be out of the office from March 18th until March 21st. I will be checking emails periodically and will attempt to reply to your message within 24 hours. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 124, Issue 19" sent on 3/19/2014 2:11:19 PM. This is the only notification you will receive while this person is away. From amber.mckenzie <@t> gastrodocs.net Wed Mar 19 16:05:25 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Mar 19 16:05:32 2014 Subject: [Histonet] Slide Consults In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC01124746F0@JERRY.Gia.com> Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Thanks! From JMitchell <@t> uwhealth.org Wed Mar 19 16:13:53 2014 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Wed Mar 19 16:13:58 2014 Subject: [Histonet] RE: Slide Consults In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC01124746F0@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC01124746F0@JERRY.Gia.com> Message-ID: <16F90B93CA23D446980B3D591FD02DAD0E576E@UWHC-MBX14.uwhis.hosp.wisc.edu> Amber: I find this EXTREMELY irritating when an outside institution either writes their accession number on the label or puts another label over the top of my hospitals labels. It is not rude to ask other hospitals to not do this; that doesn't mean they won't do it. I will forward you my slide send out form and how I phrase this request. Jean Mitchell, BS HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, March 19, 2014 4:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Consults Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Mar 19 16:15:56 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Wed Mar 19 16:16:01 2014 Subject: [Histonet] RE: Slide Consults In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC01124746F0@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC01124746F0@JERRY.Gia.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F66F2E1022@GHSEXMBX1W8K1V.geisinger.edu> I think it's pretty standard that outside institutions put their accession ID on the front of the consult slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, March 19, 2014 5:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Consults Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From Steven.Weston <@t> utas.edu.au Wed Mar 19 17:11:18 2014 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Wed Mar 19 17:11:28 2014 Subject: [Histonet] re Electron microscope choices ? Message-ID: Hi all, I'm still looking for ideas about the best types of TEM around at the moment. If you have recently purchased one or have been a regular user of a late model digital TEM please share your experience with me. regards Steve Weston Lab Manager Breathe-Well Centre of Research Excellence for Chronic Respiratory Disease. UTAS-SOM 0408990859 62264871 Steve Weston Lab Manager Breathe-Well Centre of Research Excellence for Chronic Respiratory Disease. UTAS-SOM 0408990859 62264871 From santij1 <@t> bellsouth.net Wed Mar 19 17:37:13 2014 From: santij1 <@t> bellsouth.net (JERRY SANTIAGO) Date: Wed Mar 19 17:37:19 2014 Subject: [Histonet] HT Positions in Greenville, SC Message-ID: <1395268633.8410.YahooMailNeo@web180904.mail.ne1.yahoo.com> Hello Histonetters, I have been contacted with a site that is looking to fill several HT positions at their Grenville, SC lab. If interested, please contact me and I will give you the contact information. No recruiters please! Jerry Santiago Email: santij1@bellsouth.net From Lynn.O'Donnell <@t> wchn.org Thu Mar 20 07:13:02 2014 From: Lynn.O'Donnell <@t> wchn.org (O'Donnell, Lynn M.) Date: Thu Mar 20 07:13:14 2014 Subject: [Histonet] RE: Slide Consults In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F66F2E1022@GHSEXMBX1W8K1V.geisinger.edu> References: <5A33C952BB67F4468AF1F36D739212BC01124746F0@JERRY.Gia.com> <77F52EFAB8B1694B885E277C48FCD0F66F2E1022@GHSEXMBX1W8K1V.geisinger.edu> Message-ID: I am ok with them putting the label on the front as long as it does not obscure our label. Especially since our label contains bar codes we use for tracking and storage. ______________________________ Lynn M. O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-6704 Email: lynn.o'donnell@wchn.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, March 19, 2014 17:16 To: Amber McKenzie; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide Consults I think it's pretty standard that outside institutions put their accession ID on the front of the consult slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, March 19, 2014 5:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Consults Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From becky.garrison <@t> jax.ufl.edu Thu Mar 20 08:46:28 2014 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Thu Mar 20 08:46:34 2014 Subject: [Histonet] RE: Slide Consults In-Reply-To: References: <5A33C952BB67F4468AF1F36D739212BC01124746F0@JERRY.Gia.com> <77F52EFAB8B1694B885E277C48FCD0F66F2E1022@GHSEXMBX1W8K1V.geisinger.edu> Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F2DB6A18AA@JX1B-MAIL1.umc.ufl.edu> We do not cover the referring institution label. Will place our label on front if there is clear area; if not, our label is placed on the back of the slide, at the frosted end. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Lynn M. Sent: Thursday, March 20, 2014 8:13 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide Consults I am ok with them putting the label on the front as long as it does not obscure our label. Especially since our label contains bar codes we use for tracking and storage. ______________________________ Lynn M. O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-6704 Email: lynn.o'donnell@wchn.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, March 19, 2014 17:16 To: Amber McKenzie; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Slide Consults I think it's pretty standard that outside institutions put their accession ID on the front of the consult slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, March 19, 2014 5:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Consults Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brannon <@t> alliedsearchpartners.com Thu Mar 20 08:56:35 2014 From: brannon <@t> alliedsearchpartners.com (Brannon Owens) Date: Thu Mar 20 08:56:49 2014 Subject: [Histonet] Lead Histotech opening near Orlando, FL Message-ID: We are seeking a seasoned Histotechnologist for an Orlando, FL based lab. This is a Lead position on the 3rd shift (overnight) and is a full time/permanent opportunity. Send your resume to resumes@alliedsearchpartners.com to be considered. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 F: 888.388.7572 From BMolinari <@t> texasheart.org Thu Mar 20 09:44:10 2014 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Thu Mar 20 09:44:18 2014 Subject: [Histonet] Okajima stain control Message-ID: HI, What would I use for a control for the Okajima stain (hemoglobin). This is my first time doing this stain so any advice would help as well. Thanks! Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. From Sarah.Dysart <@t> stdavids.com Thu Mar 20 10:12:52 2014 From: Sarah.Dysart <@t> stdavids.com (Sarah.Dysart@stdavids.com) Date: Thu Mar 20 10:13:00 2014 Subject: [Histonet] Melanin Bleaching... Message-ID: <34C22BB94729434598D767D3F4EB95E09839DF894E@FWDCWPMSGCMS03.hca.corpad.net> So...I have never done this before. I looked it up in the good ol' bible and found a couple protocols. The pathologist wanted to use the 10% H2O2 procedure because he thought it would be more gentle. So...the slides have been sitting in the 10% solution for 24 hours now. While it is definitely working (slow as a snail...but getting lighter...), can someone please advise on what the end point of this looks like?? Thanks!! =) Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 From joelleweaver <@t> hotmail.com Thu Mar 20 10:20:25 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Mar 20 10:20:32 2014 Subject: [Histonet] RE: Slide Consults In-Reply-To: <9E47DE9D490DCC42A2EAE94F22BF93F2DB6A18AA@JX1B-MAIL1.umc.ufl.edu> References: <5A33C952BB67F4468AF1F36D739212BC01124746F0@JERRY.Gia.com>, <77F52EFAB8B1694B885E277C48FCD0F66F2E1022@GHSEXMBX1W8K1V.geisinger.edu>, , <9E47DE9D490DCC42A2EAE94F22BF93F2DB6A18AA@JX1B-MAIL1.umc.ufl.edu> Message-ID: it doesn't bother me to have two labels/tracking numbers. I retain theirs so that they keep the liability of identification with the patient blocks and specimen source since I do not have these items to be able to cross check with the slides. The in- house ID is really just for in house processing and tracking, and to be able to use the LIS to capture for QC reports. I leave both visible though and both appear on all work-products. You just ignore the other number when filing and it seems to not be a problem. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: becky.garrison@jax.ufl.edu > To: Lynn.O'Donnell@wchn.org; histonet@lists.utsouthwestern.edu > Date: Thu, 20 Mar 2014 13:46:28 +0000 > CC: > Subject: [Histonet] RE: Slide Consults > > We do not cover the referring institution label. Will place our label on front if there is clear area; if not, our label is placed on the back of the slide, at the frosted end. > > > Becky Garrison > Pathology Supervisor > Shands Jacksonville > Jacksonville, FL 32209 > 904-244-6237, phone > 904-244-4290, fax > 904-393-3194, pager > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Lynn M. > Sent: Thursday, March 20, 2014 8:13 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Slide Consults > > I am ok with them putting the label on the front as long as it does not obscure our label. Especially since our label contains bar codes we use for tracking and storage. > > ______________________________ > Lynn M. O'Donnell, CT (ASCP), MHA > Technical Specialist, Cytology > Danbury Hospital > 203-739-6704 > Email: lynn.o'donnell@wchn.org > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. > Sent: Wednesday, March 19, 2014 17:16 > To: Amber McKenzie; Histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Slide Consults > > I think it's pretty standard that outside institutions put their accession ID on the front of the consult slides. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Wednesday, March 19, 2014 5:05 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide Consults > > > Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form? > > Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Burk <@t> pbrc.edu Thu Mar 20 10:54:31 2014 From: David.Burk <@t> pbrc.edu (David Burk) Date: Thu Mar 20 10:54:44 2014 Subject: [Histonet] Oil Red O staining question Message-ID: <281CF76A690FA74783D6EF0EC4EF243CADF1F2@pbrcas30.pbrc.edu> Experts! Is there any reason you would not consider formalin fixation followed by cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter) staining of mouse muscle? I ask as many folks seem to have difficulty in the flash freezing aspect of tissue collection and end up with lots of ice crystal damage. While the above method is still subject to freezing artifact it does at least negate the 'rushed collection' part of the process. The literature favors flash frozen tissue but we've done successful staining of liver that has been fixed then cryoprotected prior to staining and things looked fine. Is there an issue with tissue adhering to slides after sectioning or some other reason this method is not preferred? I value your opinion as I am wondering why one would choose one approach over the other. Let's pretend tissue antigenicity isn't a factor - only section quality and lipid droplet staining. Thanks for your comments! David From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Mar 20 11:00:33 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Mar 20 11:00:44 2014 Subject: [Histonet] RE: Melanin Bleaching... In-Reply-To: <34C22BB94729434598D767D3F4EB95E09839DF894E@FWDCWPMSGCMS03.hca.corpad.net> References: <34C22BB94729434598D767D3F4EB95E09839DF894E@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: Hi Sarah, I've bleached out melanin using a 5% Potassium Permangenate solution. It's very quick - about 15 to 30 minutes. Wash thoroughly in running tap water. I've done IHC after bleaching with no damage to the tissue. Hope this helps. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sarah.Dysart@stdavids.com Sent: Thursday, March 20, 2014 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Melanin Bleaching... So...I have never done this before. I looked it up in the good ol' bible and found a couple protocols. The pathologist wanted to use the 10% H2O2 procedure because he thought it would be more gentle. So...the slides have been sitting in the 10% solution for 24 hours now. While it is definitely working (slow as a snail...but getting lighter...), can someone please advise on what the end point of this looks like?? Thanks!! =) Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGleiberman <@t> buffalobiolabs.com Thu Mar 20 11:27:41 2014 From: AGleiberman <@t> buffalobiolabs.com (Anatoli Gleiberman) Date: Thu Mar 20 11:27:45 2014 Subject: [Histonet] RE: Oil Red O staining question In-Reply-To: <281CF76A690FA74783D6EF0EC4EF243CADF1F2@pbrcas30.pbrc.edu> References: <281CF76A690FA74783D6EF0EC4EF243CADF1F2@pbrcas30.pbrc.edu> Message-ID: I used successfully Oil Red both on flash frozen and formalin-fixed sucrose cryoprotected samples. Don't see any reason why not use formalin fixation. Anatoli Gleiberman, Ph.D. Director of Histopathology Buffalo Biolabs LLC 73 High Street Buffalo, NY 14203 Phone: 716-849-6810x354 e-mail: agleiberman@buffalobiolabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Burk Sent: Thursday, March 20, 2014 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O staining question Experts! Is there any reason you would not consider formalin fixation followed by cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter) staining of mouse muscle? I ask as many folks seem to have difficulty in the flash freezing aspect of tissue collection and end up with lots of ice crystal damage. While the above method is still subject to freezing artifact it does at least negate the 'rushed collection' part of the process. The literature favors flash frozen tissue but we've done successful staining of liver that has been fixed then cryoprotected prior to staining and things looked fine. Is there an issue with tissue adhering to slides after sectioning or some other reason this method is not preferred? I value your opinion as I am wondering why one would choose one approach over the other. Let's pretend tissue antigenicity isn't a factor - only section quality and lipid droplet staining. Thanks for your comments! David _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Mar 20 11:29:33 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Mar 20 11:29:39 2014 Subject: [Histonet] RE: Oil Red O staining question In-Reply-To: <281CF76A690FA74783D6EF0EC4EF243CADF1F2@pbrcas30.pbrc.edu> References: <281CF76A690FA74783D6EF0EC4EF243CADF1F2@pbrcas30.pbrc.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05671@SBS2K8.premierlab.local> David That does work, we have done it here on mouse muscle and liver, but there are some problems to fixed and then frozen tissue. The tissue on occasion does not stay on the slide as well once it sectioned, even with plus slides. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Burk Sent: Thursday, March 20, 2014 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O staining question Experts! Is there any reason you would not consider formalin fixation followed by cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter) staining of mouse muscle? I ask as many folks seem to have difficulty in the flash freezing aspect of tissue collection and end up with lots of ice crystal damage. While the above method is still subject to freezing artifact it does at least negate the 'rushed collection' part of the process. The literature favors flash frozen tissue but we've done successful staining of liver that has been fixed then cryoprotected prior to staining and things looked fine. Is there an issue with tissue adhering to slides after sectioning or some other reason this method is not preferred? I value your opinion as I am wondering why one would choose one approach over the other. Let's pretend tissue antigenicity isn't a factor - only section quality and lipid droplet staining. Thanks for your comments! David _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Thu Mar 20 11:42:07 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Thu Mar 20 11:42:12 2014 Subject: [Histonet] HistoTALK Goes to HISTOPALOOZA 2014 Message-ID: <1395333727.49904.YahooMailNeo@web121505.mail.ne1.yahoo.com> Hello Everyone - HistoTALK (www.HistoTALK.com) was invited to the Georgia Society for Histotechnology's very 1st HISTOPALOOZA. We will be there at Calloway Gardens in Pine Mt., GA from April 25 - 27 (HISTOPALOOZA 2014 dates) interviewing some of our community's most sought after speakers. For everything you ever wanted to know about GSH's way-fun and educational HISTOPALOOZA 2014 in Pine Mt., GA, go to www.HistoSEARCH.com/gsh and check it out. A BIG thank you to President Wanda Simons and the GSH Board for the invite! Yours, Dave HistoTALK From philip_manfre <@t> merck.com Thu Mar 20 11:47:44 2014 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Thu Mar 20 11:47:57 2014 Subject: [Histonet] RE: Oil Red O staining question In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E05671@SBS2K8.premierlab.local> References: <281CF76A690FA74783D6EF0EC4EF243CADF1F2@pbrcas30.pbrc.edu> <14E2C6176416974295479C64A11CB9AE019C79E05671@SBS2K8.premierlab.local> Message-ID: <558A4571351D0C42BD923F403F4198C4C36F845A75@USCTMXP51014.merck.com> We pretty much only used formalin-fixed, frozen tissue for ORO. Just don't process them - the alcohol will dissolve the lipid out. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, March 20, 2014 12:30 PM To: David Burk; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Oil Red O staining question David That does work, we have done it here on mouse muscle and liver, but there are some problems to fixed and then frozen tissue. The tissue on occasion does not stay on the slide as well once it sectioned, even with plus slides. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Burk Sent: Thursday, March 20, 2014 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O staining question Experts! Is there any reason you would not consider formalin fixation followed by cryoprotection/cryosectioning for Oil Red O (or bodipy for that matter) staining of mouse muscle? I ask as many folks seem to have difficulty in the flash freezing aspect of tissue collection and end up with lots of ice crystal damage. While the above method is still subject to freezing artifact it does at least negate the 'rushed collection' part of the process. The literature favors flash frozen tissue but we've done successful staining of liver that has been fixed then cryoprotected prior to staining and things looked fine. Is there an issue with tissue adhering to slides after sectioning or some other reason this method is not preferred? I value your opinion as I am wondering why one would choose one approach over the other. Let's pretend tissue antigenicity isn't a factor - only section quality and lipid droplet staining. Thanks for your comments! David _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rsrichmond <@t> gmail.com Thu Mar 20 11:51:17 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Mar 20 11:51:20 2014 Subject: [Histonet] Re: Slide Consults Message-ID: Amber McKenzie (where?) asks: >>Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form?<< Well, if you put the label on the back of the slide, it becomes very difficult for me to move the slide under the microscope! Surely it's time for us to get Whole Slide Imaging to replace mailing slides around, now that the units have got down to about the price of a good microscope. But at 75 I won't see it! Bob Richmond Samurai Pathologist Maryville TN From cmiller <@t> physlab.com Thu Mar 20 11:52:35 2014 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Mar 20 11:52:41 2014 Subject: [Histonet] Fungus control Message-ID: Hello wise ones! I am having an issue with yeast floating off my lab, agar grown fungus controls. Micro made us a beautiful control 5 months ago. Now all of a sudden we are seeing a significant amount of yeast floating off the control and attaching to the outer edges of the patient tissue. I thought maybe we hadn't achieved complete fixation? Now, as we cut deeper into the control block the yeast cells are breaking away? Any thoughts or suggestions? Thanks, Cheri Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From kalschev <@t> svm.vetmed.wisc.edu Thu Mar 20 12:14:26 2014 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Thu Mar 20 12:14:29 2014 Subject: [Histonet] Jim Herrick- fluoro labels Message-ID: Jim, We used to do multiple bone labels at time points in large animals. I did not do the calculations. A veterinarian is the best resource. Also, check publications by Markel.Bouvy.Manley.Zdeblick - all independent researchers that used fluorochome labels and identification using PMMA preparations. Vicki From avistarop <@t> ffyb.uba.ar Thu Mar 20 12:57:32 2014 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Thu Mar 20 12:57:43 2014 Subject: [Histonet] IHQ Antibody IgD Message-ID: <730c6bdea1cc384ca4d0420be7edd0d7.squirrel@huemul.ffyb.uba.ar> Hello to everyone!!! Has anyone used this antibody ? Novocastra Mouse Monoclonal Antibody Inmuneglobulin D This antibody is used on FFPE tissue. Could I please get a copy of your protocols? I need to know retrieval epitope time and method. Thank you so much Aldana Vistarop From VMiller <@t> summitpathology.com Thu Mar 20 13:17:22 2014 From: VMiller <@t> summitpathology.com (Virginia Miller) Date: Thu Mar 20 13:17:30 2014 Subject: [Histonet] bone marrow and clot and core Message-ID: <48BF0FEE1EC4D04C8D5B571A75B4786602F6A960DC@spmbx01.summitpathology.int> Hi All , I was wondering if anyone had any advise on getting clots and cores to stay put? We are using plus slides and giving the slides extra oven time and we are still having issues. So if anyone has some histomagic up their sleeve regarding this issue it would be appreciated. Ginny Miller HT (ASCP) (970)672-6183 Summit Pathology 5802 Wright dr. Loveland co 80538 From akelley <@t> path.wustl.edu Thu Mar 20 13:21:52 2014 From: akelley <@t> path.wustl.edu (Kelley, Amanda) Date: Thu Mar 20 13:22:28 2014 Subject: [Histonet] Re: Slide Consults In-Reply-To: References: Message-ID: Amber, My lab buys half size colored slide labels and we place them on the bottom of the slide, they are just a little bit bigger than the size of the +'s on plus slides. We have a printer for consult slide labels which when they are received we print these half labels. They do not obscure the labels from the submitting lab, and we can scan our slides for future reference and electronically attach them to the case, with both numbers intact. Amanda Dermatopathology center Washington University Medical School in St. Louis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Thursday, March 20, 2014 11:51 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Slide Consults Amber McKenzie (where?) asks: >>Would it be rude to ask other hospitals that review my slides to NOT WRITE their accession number on the front of my slides, but instead put their accession number on the back of my slides? Sometimes, I get slides back that have stickers on the front under my accession number or they'll hand write their accession number under mine. I feel like I should scribble out the other institution number so that it doesn't confuse any of us refilling slides. What are your thoughts? Would anyone like to share their slide send out form?<< Well, if you put the label on the back of the slide, it becomes very difficult for me to move the slide under the microscope! Surely it's time for us to get Whole Slide Imaging to replace mailing slides around, now that the units have got down to about the price of a good microscope. But at 75 I won't see it! Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From NateUT06 <@t> hotmail.com Thu Mar 20 14:46:48 2014 From: NateUT06 <@t> hotmail.com (Nathan Sires) Date: Thu Mar 20 14:46:55 2014 Subject: [Histonet] Fungus Controls (Nathan Sires) Message-ID: Cheryl, If you are having trouble with the yeast floating down the slide and onto your patient tissue, you can always run the control and the patient tissue on separate slides. Running separate controls is always a good idea when searching for microorganisms (GMS, AFB, or PAS-F stains). Also, I have made my own fungus controls before and it is actually really easy! I also didn?t have any trouble with the fungus floating with this method. All I did was get a sterile urine container, place an orange peel (rind) inside the container, put the lid back on, poke a couple small holes in the top of the lid, and place the container in a window sill somewhere in the laboratory. In a couple of days to a week, you will have some nice fungus (mold) growing on the rind. Once you are done, you can take the rind and cut it into smaller pieces and embed them in separate blocks. This will yield you a whole bunch of fungus controls. : ) Nathan Sires, BA, HT(ASCP) Histology Supervisor McAllen Medical Center 956-632-4255 Histology 512-658-2897 Cell From awatanabe <@t> tgen.org Thu Mar 20 18:32:59 2014 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Thu Mar 20 18:33:09 2014 Subject: [Histonet] Cassette/slide writers and embedding center Message-ID: Hello everyone, I am doing a product survey of sorts and was just wondering which cassette writer, slide writer and embedding center you were using. I don?t need a huge product review but any details worth noting would be good. I am equipment hunting for a lab that will be doing 30-60 clinical samples/month with a potential to increase 4 fold. Any info would be greatly appreciated. Aprill Watanabe, B.S. Laboratory Coordinator The Dorrance Clinical Laboratory at TGen, Histopathology Laboratory Integrated Cancer Genomics Division Macromolecular Analyte Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Office: 602-343-8822 DCL Lab: 602-343-8796 Fax: 602-343-8717 Cell: 602-481-8654 email: awatanabe@tgen.org website: www.tgen.org From carrie.lindberg <@t> lourdes.com Fri Mar 21 09:32:40 2014 From: carrie.lindberg <@t> lourdes.com (Lindberg, Carrie A) Date: Fri Mar 21 09:32:44 2014 Subject: [Histonet] RE: Cassette/slide writers and embedding center In-Reply-To: References: Message-ID: <25737B4BBF458340B2F26F472C8F760F5267A408@TX1P03DAG0104.apptixhealth.net> My lab just implemented Thermo Fisher's Print-mate and slide-mates, I love them! The embedding center is the Thermo Histocenter. I have no complaints about it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Aprill Watanabe Sent: Thursday, March 20, 2014 7:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette/slide writers and embedding center Hello everyone, I am doing a product survey of sorts and was just wondering which cassette writer, slide writer and embedding center you were using. I don't need a huge product review but any details worth noting would be good. I am equipment hunting for a lab that will be doing 30-60 clinical samples/month with a potential to increase 4 fold. Any info would be greatly appreciated. Aprill Watanabe, B.S. Laboratory Coordinator The Dorrance Clinical Laboratory at TGen, Histopathology Laboratory Integrated Cancer Genomics Division Macromolecular Analyte Processing Center (MAPC) Translational Genomics Research Institute (TGen) 445 North 5th Street Phoenix, AZ 85004 Office: 602-343-8822 DCL Lab: 602-343-8796 Fax: 602-343-8717 Cell: 602-481-8654 email: awatanabe@tgen.org website: www.tgen.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From CDavis <@t> che-east.org Fri Mar 21 12:03:35 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Fri Mar 21 12:03:01 2014 Subject: [Histonet] specimen marking ink Message-ID: Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From king.laurie <@t> marshfieldclinic.org Fri Mar 21 12:45:48 2014 From: king.laurie <@t> marshfieldclinic.org (King, Laurie J) Date: Fri Mar 21 12:45:54 2014 Subject: [Histonet] Leica Reichert Jung Cryocut 1800 Message-ID: <7578207839F50248A7A6CD33517295EA4F13B680@MCL-EXMB03.mfldclin.org> Hello all, Looking for a manual for a Leica Reichert Jung Cryocut 1800. Laurie ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From Wanda.Smith <@t> HCAhealthcare.com Fri Mar 21 14:37:59 2014 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Fri Mar 21 14:38:07 2014 Subject: [Histonet] RE: specimen marking ink In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27EFDDFC29@NADCWPMSGCMS03.hca.corpad.net> Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue. Don't know where it came from and don't know where it went!!! Happy Friday and Happy Weekend to everyone!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Friday, March 21, 2014 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen marking ink Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From immunoqueen <@t> yahoo.com Fri Mar 21 15:06:46 2014 From: immunoqueen <@t> yahoo.com (Jennifer Leigh) Date: Fri Mar 21 15:06:49 2014 Subject: [Histonet] IHC on paraffin embedded skin tissue! Message-ID: <1395432406.35742.YahooMailNeo@web120703.mail.ne1.yahoo.com> ?????? Histonetters- ? ?????????? I have a question about mouse skin samples----an IHC experiment has gone wrong and I am not sure why. I have a set of paraffin embedded mouse skin samples from an inflammatory model that were stained for F4/80. The end result is no color development after the addition of DAB. I use the DAB tablets from Sigma using the TBS buffer to dissolve the tablet. There is no color development, not even non-specific staining. Any ideas what the problem might be?????? I also performed a Proteinase K antigen retrevial step (using the ready made solution from Dako) for 15 minutest at 37 C as I was instructed to do. Thank you in advance for any insight! ? Jennifer Oskins Jennifer L. Oskins "Until one has loved an animal, part of their soul remains unawakened....." From histotalk <@t> yahoo.com Fri Mar 21 18:02:29 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Fri Mar 21 18:05:29 2014 Subject: [Histonet] RE: specimen marking ink In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27EFDDFC29@NADCWPMSGCMS03.hca.corpad.net> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27EFDDFC29@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <1395442949.1599.YahooMailNeo@web121504.mail.ne1.yahoo.com> Actually, I have a tattoo supplier four miles away from me. Several years ago, I went to their warehouse to take a look an possibly make a purchase. As it turned out, I had to buy a gallon of each color and it worked out to be more than the standard tissue inks sold by the histology vendors.? Next stop - tattoo shop. Same story. The artist would sell a small 8 oz. bottle at cost, but it was still just a bit more than our vendors. You can use Pelican drawing ink. It comes in several colors. It's around five something for an ounce+, much more than Davidson or one of the others. Maybe someone will also chime in. Dave? On Friday, March 21, 2014 3:39 PM, "Wanda.Smith@HCAhealthcare.com" wrote: Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue.? Don't know where it came from and don't know where it went!!! Happy Friday and Happy Weekend to everyone!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Friday, March 21, 2014 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen marking ink Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Fri Mar 21 20:26:05 2014 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Mar 21 20:26:19 2014 Subject: [Histonet] RE: specimen marking ink In-Reply-To: <1395442949.1599.YahooMailNeo@web121504.mail.ne1.yahoo.com> References: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27EFDDFC29@NADCWPMSGCMS03.hca.corpad.net> <1395442949.1599.YahooMailNeo@web121504.mail.ne1.yahoo.com> Message-ID: <532CE6AD.8050806@shaw.ca> You can buy tattoo ink in a bewildering array of colours from ebay quite cheaply. Many years ago I tried to buy tattoo ink from a tattoo parlour, but they refused to sell it to me as they were concerned that it would be used for rubbing into scratches and cuts for home made tattoos. I was in my mid-50's at the time. Perhaps I looked strange with my beard and long hair! I decided to buy the commercial tattoo ink kits from lab suppliers for mixed colours, but used laundry blueing for most margins and kept the tattoo inks for those occasions when it was important to know which margin was which. Bryan Llewellyn David Kemler wrote: > Actually, I have a tattoo supplier four miles away from me. Several years ago, I went to their warehouse to take a look an possibly make a purchase. As it turned out, I had to buy a gallon of each color and it worked out to be more than the standard tissue inks sold by the histology vendors. > > Next stop - tattoo shop. Same story. The artist would sell a small 8 oz. bottle at cost, but it was still just a bit more than our vendors. > > You can use Pelican drawing ink. It comes in several colors. It's around five something for an ounce+, much more than Davidson or one of the others. > > Maybe someone will also chime in. > > Dave > > > > On Friday, March 21, 2014 3:39 PM, "Wanda.Smith@HCAhealthcare.com" wrote: > > Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue. Don't know where it came from and don't know where it went!!! > Happy Friday and Happy Weekend to everyone!!! > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > Sent: Friday, March 21, 2014 1:04 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] specimen marking ink > > Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From latecor <@t> montevideo.com.uy Fri Mar 21 21:44:20 2014 From: latecor <@t> montevideo.com.uy (C.D.G.) Date: Fri Mar 21 20:42:12 2014 Subject: [Histonet] IHC on paraffin embedded skin tissue! In-Reply-To: <1395432406.35742.YahooMailNeo@web120703.mail.ne1.yahoo.com> References: <1395432406.35742.YahooMailNeo@web120703.mail.ne1.yahoo.com> Message-ID: <201403212344200234.000D0792@smtp.montevideo.com.uy> Jennifer be sure to add three or four drops of H2O2 to the solution. Maybe the lack of oxidation of the chromogen is the cause of no color development. Best luck, Carlos.- >?????? Histonetters- ? ?????????? I have a question about mouse skin >samples----an IHC experiment has gone wrong and I am not sure why. I have >a set of paraffin embedded mouse skin samples from an inflammatory model >that were stained for F4/80. The end result is no color development after >the addition of DAB. I use the DAB tablets from Sigma using the TBS buffer >to dissolve the tablet. There is no color development, not even >non-specific staining. Any ideas what the problem might be?????? I also >performed a Proteinase K antigen retrevial step (using the ready made >solution from Dako) for 15 minutest at 37 C as I was instructed to do. >Thank you in advance for any insight! ? Jennifer Oskins Jennifer L. >Oskins "Until one has loved an animal, part of their soul remains >unawakened....." >_______________________________________________ >Histonet mailing list > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj <@t> pigsqq.org Sat Mar 22 18:21:48 2014 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Sat Mar 22 18:22:11 2014 Subject: [Histonet] specimen marking ink In-Reply-To: References: Message-ID: <532E1B0C.3040607@pigsqq.org> Tattooing is not only for dissidents, miscreants, the wayward, and the Llewellyn-ites among us. Sheep, rabbits, pigs, cattle, horses, stoats, and goats can be tattooed also, not so much of an expression of individuality as the need for permanent identification. http://www.enasco.com/c/farmandranch/Livestock%20Identification/Tattooing/ On 3:59 AM, Davis, Cassie wrote: > Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > From drmoses111 <@t> comcast.net Sat Mar 22 20:38:17 2014 From: drmoses111 <@t> comcast.net (drmoses111@comcast.net) Date: Sat Mar 22 20:38:46 2014 Subject: [Histonet] Help kappa and lambda IHC on bone marrow bxs In-Reply-To: <51367161.2162870.1395537497986.JavaMail.root@comcast.net> Message-ID: <1849852922.2166556.1395538697875.JavaMail.root@comcast.net> Our lab has started?using Immunocal decal solution on our bone marrows. Most of our antibodies have improved except kappa and lambda. We do not do ISH. Kappa and lambda staining?in the tonsil controls is good, The bone marrows?are?now very overstrained. We use DAKO polyclonals? at 1:10,000 with protease1 on the Ventana Ultra. Does anyone have a procedure? ----- Original Message ----- ? ? From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 124, Issue 24 Send Histonet mailing list submissions to ????????histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ????????http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ????????histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ????????histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ?? 1. specimen marking ink (Davis, Cassie) ?? 2. Leica Reichert Jung Cryocut 1800 (King, Laurie J) ?? 3. RE: specimen marking ink (Wanda.Smith@HCAhealthcare.com) ?? 4. IHC on paraffin embedded skin tissue! (Jennifer Leigh) ?? 5. Re: RE: specimen marking ink (David Kemler) ?? 6. Re: RE: specimen marking ink (Bryan Llewellyn) ?? 7. Re: IHC on paraffin embedded skin tissue! (C.D.G.) ---------------------------------------------------------------------- Message: 1 Date: Fri, 21 Mar 2014 13:03:35 -0400 From: "Davis, Cassie" Subject: [Histonet] specimen marking ink To: "histonet@lists.utsouthwestern.edu" ???????? Message-ID: ???????? Content-Type: text/plain; charset="iso-8859-1" Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ? ------------------------------ Message: 2 Date: Fri, 21 Mar 2014 17:45:48 +0000 From: "King, Laurie J" Subject: [Histonet] Leica Reichert Jung Cryocut 1800 To: "'histonet@lists.utsouthwestern.edu' ????????(histonet@lists.utsouthwestern.edu)" ???????? Message-ID: ????????<7578207839F50248A7A6CD33517295EA4F13B680@MCL-EXMB03.mfldclin.org> Content-Type: text/plain; charset="us-ascii" Hello all, Looking for a manual for a Leica Reichert Jung Cryocut 1800. Laurie ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. ?If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. ?Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. ?Thank you for your cooperation. ------------------------------ Message: 3 Date: Fri, 21 Mar 2014 14:37:59 -0500 From: Subject: [Histonet] RE: specimen marking ink To: , Message-ID: ????????<9E2D36CE2D7CBA4A94D9B22E8328A3BA27EFDDFC29@NADCWPMSGCMS03.hca.corpad.net> ???????? Content-Type: text/plain; charset="iso-8859-1" Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue. ?Don't know where it came from and don't know where it went!!! Happy Friday and Happy Weekend to everyone!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Friday, March 21, 2014 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen marking ink Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 21 Mar 2014 13:06:46 -0700 (PDT) From: Jennifer Leigh Subject: [Histonet] IHC on paraffin embedded skin tissue! To: Histonet Netserver Message-ID: ????????<1395432406.35742.YahooMailNeo@web120703.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ?????? Histonetters- ? ?????????? I have a question about mouse skin samples----an IHC experiment has gone wrong and I am not sure why. I have a set of paraffin embedded mouse skin samples from an inflammatory model that were stained for F4/80. The end result is no color development after the addition of DAB. I use the DAB tablets from Sigma using the TBS buffer to dissolve the tablet. There is no color development, not even non-specific staining. Any ideas what the problem might be?????? I also performed a Proteinase K antigen retrevial step (using the ready made solution from Dako) for 15 minutest at 37 C as I was instructed to do. Thank you in advance for any insight! ? Jennifer Oskins Jennifer L. Oskins "Until one has loved an animal, part of their soul remains unawakened....." ------------------------------ Message: 5 Date: Fri, 21 Mar 2014 16:02:29 -0700 (PDT) From: David Kemler Subject: Re: [Histonet] RE: specimen marking ink To: "Wanda.Smith@HCAhealthcare.com" , ????????"CDavis@che-east.org" , ????????"histonet@lists.utsouthwestern.edu" ???????? Message-ID: ????????<1395442949.1599.YahooMailNeo@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Actually, I have a tattoo supplier four miles away from me. Several years ago, I went to their warehouse to take a look an possibly make a purchase. As it turned out, I had to buy a gallon of each color and it worked out to be more than the standard tissue inks sold by the histology vendors.? Next stop - tattoo shop. Same story. The artist would sell a small 8 oz. bottle at cost, but it was still just a bit more than our vendors. You can use Pelican drawing ink. It comes in several colors. It's around five something for an ounce+, much more than Davidson or one of the others. Maybe someone will also chime in. Dave? On Friday, March 21, 2014 3:39 PM, "Wanda.Smith@HCAhealthcare.com" wrote: ? Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue.? Don't know where it came from and don't know where it went!!! Happy Friday and Happy Weekend to everyone!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Friday, March 21, 2014 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen marking ink Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 21 Mar 2014 18:26:05 -0700 From: Bryan Llewellyn Subject: Re: [Histonet] RE: specimen marking ink To: histonet@lists.utsouthwestern.edu Message-ID: <532CE6AD.8050806@shaw.ca> Content-Type: text/plain; charset=ISO-8859-1; format=flowed You can buy tattoo ink in a bewildering array of colours from ebay quite cheaply. Many years ago I tried to buy tattoo ink from a tattoo parlour, but they refused to sell it to me as they were concerned that it would be used for rubbing into scratches and cuts for home made tattoos. I was in my mid-50's at the time. Perhaps I looked strange with my beard and long hair! I decided to buy the commercial tattoo ink kits from lab suppliers for mixed colours, but used laundry blueing for most margins and kept the tattoo inks for those occasions when it was important to know which margin was which. Bryan Llewellyn David Kemler wrote: > Actually, I have a tattoo supplier four miles away from me. Several years ago, I went to their warehouse to take a look an possibly make a purchase. As it turned out, I had to buy a gallon of each color and it worked out to be more than the standard tissue inks sold by the histology vendors. > > Next stop - tattoo shop. Same story. The artist would sell a small 8 oz. bottle at cost, but it was still just a bit more than our vendors. > > You can use Pelican drawing ink. It comes in several colors. It's around five something for an ounce+, much more than Davidson or one of the others. > > Maybe someone will also chime in. > > Dave > > > > On Friday, March 21, 2014 3:39 PM, "Wanda.Smith@HCAhealthcare.com" wrote: > > Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue. ?Don't know where it came from and don't know where it went!!! > Happy Friday and Happy Weekend to everyone!!! > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC ?29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > Sent: Friday, March 21, 2014 1:04 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] specimen marking ink > > Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Fri, 21 Mar 2014 23:44:20 -0300 From: "C.D.G." Subject: Re: [Histonet] IHC on paraffin embedded skin tissue! To: Histonet@lists.utsouthwestern.edu Cc: Histonet@lists.utsouthwestern.edu Message-ID: <201403212344200234.000D0792@smtp.montevideo.com.uy> Content-Type: text/plain; charset="ISO-8859-1" ?Jennifer be sure to add three or four drops of H2O2 to the solution. Maybe the lack ?of oxidation of the chromogen is the cause of no color development. ?Best luck, ?Carlos.- >?????? Histonetters- ? ?????????? I have a question about mouse skin >samples----an IHC experiment has gone wrong and I am not sure why. I have >a set of paraffin embedded mouse skin samples from an inflammatory model >that were stained for F4/80. The end result is no color development after >the addition of DAB. I use the DAB tablets from Sigma using the TBS buffer >to dissolve the tablet. There is no color development, not even >non-specific staining. Any ideas what the problem might be?????? I also >performed a Proteinase K antigen retrevial step (using the ready made >solution from Dako) for 15 minutest at 37 C as I was instructed to do. >Thank you in advance for any insight! ? Jennifer Oskins Jennifer L. >Oskins "Until one has loved an animal, part of their soul remains >unawakened....." >_______________________________________________ >Histonet mailing list > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 124, Issue 24 ***************************************** From bethcoxx <@t> gmail.com Sun Mar 23 17:57:19 2014 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Sun Mar 23 17:57:24 2014 Subject: [Histonet] Anyone in Uganda?? Message-ID: <532F66CF.4080804@gmail.com> Is there anyone on the Histonet in Uganda?? I have questions regarding licensing/certification/training for lab people there. Please feel free to contact me directly. Beth Cox, HTL/SCT(ASCP)QIHC From histotalk <@t> yahoo.com Mon Mar 24 05:56:52 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Mon Mar 24 05:56:57 2014 Subject: [Histonet] HistoTALK Guest PAM BARKER Message-ID: <1395658612.31892.YahooMailNeo@web121501.mail.ne1.yahoo.com> Hello Everyone - HistoTALK welcomes Pam Barker from RELIA to the show. Pam has some fantastic tips she shares with us when it comes to finding that "just" right Histology position. Join us at www.HistoTALK.com Yours, David From king.laurie <@t> marshfieldclinic.org Mon Mar 24 07:27:20 2014 From: king.laurie <@t> marshfieldclinic.org (King, Laurie J) Date: Mon Mar 24 07:27:25 2014 Subject: [Histonet] CM1800 manual Message-ID: <7578207839F50248A7A6CD33517295EA4F13B94E@MCL-EXMB03.mfldclin.org> Got the manual I asked for, thanks everybody! Laurie ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From algranth <@t> email.arizona.edu Mon Mar 24 09:50:17 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Mar 24 09:50:22 2014 Subject: [Histonet] Anyone in Uganda?? In-Reply-To: <532F66CF.4080804@gmail.com> References: <532F66CF.4080804@gmail.com> Message-ID: Hey Beth, Aren't you about ready to go? Won't the Rotary group take care of your licensing? Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From Andrea.X.Dejager <@t> kp.org Mon Mar 24 10:01:25 2014 From: Andrea.X.Dejager <@t> kp.org (Andrea.X.Dejager@kp.org) Date: Mon Mar 24 10:02:01 2014 Subject: [Histonet] Re: Histonet Digest, Vol 124, Issue 25 In-Reply-To: <201403231703.s2NH39Fa009617@cscrdsmrp113.kp.org> References: <201403231703.s2NH39Fa009617@cscrdsmrp113.kp.org> Message-ID: We get our tattoo dyes from Royal Marker - www.royalmarker.com Have been using them for years and are very happy with the results. These do not dry out and cake as often. Andrea De Jager, H.T. ASCP Histology Manager, Regional Reference Lab Kaiser Permanente - Colorado Phone: 303-404-4152 Fax: 303-404-4161 email: ANDREA.X.DEJAGER@KP.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 03/23/2014 11:03 AM Subject: Histonet Digest, Vol 124, Issue 25 Sent by: histonet-bounces@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: specimen marking ink (E. Wayne Johnson ???) 2. Help kappa and lambda IHC on bone marrow bxs (drmoses111@comcast.net) ---------------------------------------------------------------------- Message: 1 Date: Sun, 23 Mar 2014 07:21:48 +0800 From: "E. Wayne Johnson ???" Subject: Re: [Histonet] specimen marking ink To: "Davis, Cassie" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <532E1B0C.3040607@pigsqq.org> Content-Type: text/plain; charset=UTF-8; format=flowed Tattooing is not only for dissidents, miscreants, the wayward, and the Llewellyn-ites among us. Sheep, rabbits, pigs, cattle, horses, stoats, and goats can be tattooed also, not so much of an expression of individuality as the need for permanent identification. http://www.enasco.com/c/farmandranch/Livestock%20Identification/Tattooing/ On 3:59 AM, Davis, Cassie wrote: > Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > ------------------------------ Message: 2 Date: Sun, 23 Mar 2014 01:38:17 +0000 (UTC) From: drmoses111@comcast.net Subject: [Histonet] Help kappa and lambda IHC on bone marrow bxs To: histonet@lists.utsouthwestern.edu Message-ID: <1849852922.2166556.1395538697875.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 Our lab has started? using Immunocal decal solution on our bone marrows. Most of our antibodies have improved except kappa and lambda. We do not do ISH. Kappa and lambda staining? in the tonsil controls is good, The bone marrows? are? now very overstrained. We use DAKO polyclonals? at 1:10,000 with protease1 on the Ventana Ultra. Does anyone have a procedure? ----- Original Message ----- ? ? From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 124, Issue 24 Send Histonet mailing list submissions to ? ? ? ? ? ? ? ? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ? ? ? ? ? ? ? ? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ? ? ? ? ? ? ? ? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ? ? ? ? ? ? ? ? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? ? 1. specimen marking ink (Davis, Cassie) ? ? 2. Leica Reichert Jung Cryocut 1800 (King, Laurie J) ? ? 3. RE: specimen marking ink (Wanda.Smith@HCAhealthcare.com) ? ? 4. IHC on paraffin embedded skin tissue! (Jennifer Leigh) ? ? 5. Re: RE: specimen marking ink (David Kemler) ? ? 6. Re: RE: specimen marking ink (Bryan Llewellyn) ? ? 7. Re: IHC on paraffin embedded skin tissue! (C.D.G.) ---------------------------------------------------------------------- Message: 1 Date: Fri, 21 Mar 2014 13:03:35 -0400 From: "Davis, Cassie" Subject: [Histonet] specimen marking ink To: "histonet@lists.utsouthwestern.edu" ? ? ? ? ? ? ? ? Message-ID: ? ? ? ? ? ? ? ? Content-Type: text/plain; charset="iso-8859-1" Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. ? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ? ------------------------------ Message: 2 Date: Fri, 21 Mar 2014 17:45:48 +0000 From: "King, Laurie J" Subject: [Histonet] Leica Reichert Jung Cryocut 1800 To: "'histonet@lists.utsouthwestern.edu' ? ? ? ? ? ? ? ? (histonet@lists.utsouthwestern.edu)" ? ? ? ? ? ? ? ? Message-ID: ? ? ? ? ? ? ? ? <7578207839F50248A7A6CD33517295EA4F13B680@MCL-EXMB03.mfldclin.org> Content-Type: text/plain; charset="us-ascii" Hello all, Looking for a manual for a Leica Reichert Jung Cryocut 1800. Laurie ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. ? If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. ? Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. ? Thank you for your cooperation. ------------------------------ Message: 3 Date: Fri, 21 Mar 2014 14:37:59 -0500 From: Subject: [Histonet] RE: specimen marking ink To: , Message-ID: ? ? ? ? ? ? ? ? <9E2D36CE2D7CBA4A94D9B22E8328A3BA27EFDDFC29@NADCWPMSGCMS03.hca.corpad.net> ? ? ? ? ? ? ? ? Content-Type: text/plain; charset="iso-8859-1" Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue. ? Don't know where it came from and don't know where it went!!! Happy Friday and Happy Weekend to everyone!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC??? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Friday, March 21, 2014 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen marking ink Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. ? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 21 Mar 2014 13:06:46 -0700 (PDT) From: Jennifer Leigh Subject: [Histonet] IHC on paraffin embedded skin tissue! To: Histonet Netserver Message-ID: ? ? ? ? ? ? ? ? <1395432406.35742.YahooMailNeo@web120703.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ?????????????????? Histonetters- ??? ?????????????????????????????? I have a question about mouse skin samples----an IHC experiment has gone wrong and I am not sure why. I have a set of paraffin embedded mouse skin samples from an inflammatory model that were stained for F4/80. The end result is no color development after the addition of DAB. I use the DAB tablets from Sigma using the TBS buffer to dissolve the tablet. There is no color development, not even non-specific staining. Any ideas what the problem might be???????? I also performed a Proteinase K antigen retrevial step (using the ready made solution from Dako) for 15 minutest at 37 C as I was instructed to do. Thank you in advance for any insight! ??? Jennifer Oskins Jennifer L. Oskins "Until one has loved an animal, part of their soul remains unawakened....." ------------------------------ Message: 5 Date: Fri, 21 Mar 2014 16:02:29 -0700 (PDT) From: David Kemler Subject: Re: [Histonet] RE: specimen marking ink To: "Wanda.Smith@HCAhealthcare.com" , ? ? ? ? ? ? ? ? "CDavis@che-east.org" , ? ? ? ? ? ? ? ? "histonet@lists.utsouthwestern.edu" ? ? ? ? ? ? ? ? Message-ID: ? ? ? ? ? ? ? ? <1395442949.1599.YahooMailNeo@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Actually, I have a tattoo supplier four miles away from me. Several years ago, I went to their warehouse to take a look an possibly make a purchase. As it turned out, I had to buy a gallon of each color and it worked out to be more than the standard tissue inks sold by the histology vendors.??? Next stop - tattoo shop. Same story. The artist would sell a small 8 oz. bottle at cost, but it was still just a bit more than our vendors. You can use Pelican drawing ink. It comes in several colors. It's around five something for an ounce+, much more than Davidson or one of the others. Maybe someone will also chime in. Dave??? On Friday, March 21, 2014 3:39 PM, "Wanda.Smith@HCAhealthcare.com" wrote: ? Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue.??? Don't know where it came from and don't know where it went!!! Happy Friday and Happy Weekend to everyone!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC??? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Friday, March 21, 2014 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen marking ink Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential.??? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 21 Mar 2014 18:26:05 -0700 From: Bryan Llewellyn Subject: Re: [Histonet] RE: specimen marking ink To: histonet@lists.utsouthwestern.edu Message-ID: <532CE6AD.8050806@shaw.ca> Content-Type: text/plain; charset=ISO-8859-1; format=flowed You can buy tattoo ink in a bewildering array of colours from ebay quite cheaply. Many years ago I tried to buy tattoo ink from a tattoo parlour, but they refused to sell it to me as they were concerned that it would be used for rubbing into scratches and cuts for home made tattoos. I was in my mid-50's at the time. Perhaps I looked strange with my beard and long hair! I decided to buy the commercial tattoo ink kits from lab suppliers for mixed colours, but used laundry blueing for most margins and kept the tattoo inks for those occasions when it was important to know which margin was which. Bryan Llewellyn David Kemler wrote: > Actually, I have a tattoo supplier four miles away from me. Several years ago, I went to their warehouse to take a look an possibly make a purchase. As it turned out, I had to buy a gallon of each color and it worked out to be more than the standard tissue inks sold by the histology vendors. > > Next stop - tattoo shop. Same story. The artist would sell a small 8 oz. bottle at cost, but it was still just a bit more than our vendors. > > You can use Pelican drawing ink. It comes in several colors. It's around five something for an ounce+, much more than Davidson or one of the others. > > Maybe someone will also chime in. > > Dave > > > > On Friday, March 21, 2014 3:39 PM, "Wanda.Smith@HCAhealthcare.com" wrote: > > Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue. ? Don't know where it came from and don't know where it went!!! > Happy Friday and Happy Weekend to everyone!!! > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC ? 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > Sent: Friday, March 21, 2014 1:04 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] specimen marking ink > > Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. ? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Fri, 21 Mar 2014 23:44:20 -0300 From: "C.D.G." Subject: Re: [Histonet] IHC on paraffin embedded skin tissue! To: Histonet@lists.utsouthwestern.edu Cc: Histonet@lists.utsouthwestern.edu Message-ID: <201403212344200234.000D0792@smtp.montevideo.com.uy> Content-Type: text/plain; charset="ISO-8859-1" ? Jennifer be sure to add three or four drops of H2O2 to the solution. Maybe the lack ? of oxidation of the chromogen is the cause of no color development. ? Best luck, ? Carlos.- >?????????????????? Histonetters- ??? ?????????????????????????????? I have a question about mouse skin >samples----an IHC experiment has gone wrong and I am not sure why. I have >a set of paraffin embedded mouse skin samples from an inflammatory model >that were stained for F4/80. The end result is no color development after >the addition of DAB. I use the DAB tablets from Sigma using the TBS buffer >to dissolve the tablet. There is no color development, not even >non-specific staining. Any ideas what the problem might be???????? I also >performed a Proteinase K antigen retrevial step (using the ready made >solution from Dako) for 15 minutest at 37 C as I was instructed to do. >Thank you in advance for any insight! ??? Jennifer Oskins Jennifer L. >Oskins "Until one has loved an animal, part of their soul remains >unawakened....." >_______________________________________________ >Histonet mailing list > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 124, Issue 24 ***************************************** ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 124, Issue 25 ***************************************** From biohm <@t> verizon.net Mon Mar 24 12:01:46 2014 From: biohm <@t> verizon.net (Douglas J. Foster) Date: Mon Mar 24 12:01:58 2014 Subject: [Histonet] Blade for AO 820 Microtome Message-ID: Hello Histonet, I ran across Histonet after seeing a YouTube by Donna Emge. Could anyone suggest a brand and a source for an American Optical 820 microtome blade? Many thanks, Doug Foster From David.Burk <@t> pbrc.edu Mon Mar 24 14:17:19 2014 From: David.Burk <@t> pbrc.edu (David Burk) Date: Mon Mar 24 14:17:26 2014 Subject: [Histonet] RE: Oil Red O staining question In-Reply-To: <558A4571351D0C42BD923F403F4198C4C36F845A75@USCTMXP51014.merck.com> References: <281CF76A690FA74783D6EF0EC4EF243CADF1F2@pbrcas30.pbrc.edu> <14E2C6176416974295479C64A11CB9AE019C79E05671@SBS2K8.premierlab.local> <558A4571351D0C42BD923F403F4198C4C36F845A75@USCTMXP51014.merck.com> Message-ID: <281CF76A690FA74783D6EF0EC4EF243CADF272@pbrcas30.pbrc.edu> I just wanted to say "Thanks!" to all those who responded to my question on ORO staining formalin fixed cryoprotected mouse tissue. The general consensus was that it was perfectly fine to do so with the caveat that sometimes sections don't want to stick to slides. Thanks again! David From Richard.Cartun <@t> hhchealth.org Mon Mar 24 14:50:43 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Mar 24 14:50:49 2014 Subject: [Histonet] RE: Help kappa and lambda IHC on bone marrow bxs In-Reply-To: <1849852922.2166556.1395538697875.JavaMail.root@comcast.net> References: <51367161.2162870.1395537497986.JavaMail.root@comcast.net> <1849852922.2166556.1395538697875.JavaMail.root@comcast.net> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E02291B6D@HHCEXCHMB03.hhcsystem.org> I would recommend using higher dilutions of your primary antibodies (try 1:20,000). As you might expect, it's not unusual to see a lot of background immunoglobulin immunoreactivity in bone marrow specimens. We actually have 2 antibody dilutions for both Kappa and Lambda that we run on the Leica Bond Max; a routine dilution and then a higher one for these types of situations. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of drmoses111@comcast.net Sent: Saturday, March 22, 2014 9:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help kappa and lambda IHC on bone marrow bxs Our lab has started using Immunocal decal solution on our bone marrows. Most of our antibodies have improved except kappa and lambda. We do not do ISH. Kappa and lambda staining in the tonsil controls is good, The bone marrows are now very overstrained. We use DAKO polyclonals at 1:10,000 with protease1 on the Ventana Ultra. Does anyone have a procedure? ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 124, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. specimen marking ink (Davis, Cassie) 2. Leica Reichert Jung Cryocut 1800 (King, Laurie J) 3. RE: specimen marking ink (Wanda.Smith@HCAhealthcare.com) 4. IHC on paraffin embedded skin tissue! (Jennifer Leigh) 5. Re: RE: specimen marking ink (David Kemler) 6. Re: RE: specimen marking ink (Bryan Llewellyn) 7. Re: IHC on paraffin embedded skin tissue! (C.D.G.) ---------------------------------------------------------------------- Message: 1 Date: Fri, 21 Mar 2014 13:03:35 -0400 From: "Davis, Cassie" Subject: [Histonet] specimen marking ink To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 2 Date: Fri, 21 Mar 2014 17:45:48 +0000 From: "King, Laurie J" Subject: [Histonet] Leica Reichert Jung Cryocut 1800 To: "'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)" Message-ID: <7578207839F50248A7A6CD33517295EA4F13B680@MCL-EXMB03.mfldclin.org> Content-Type: text/plain; charset="us-ascii" Hello all, Looking for a manual for a Leica Reichert Jung Cryocut 1800. Laurie ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ------------------------------ Message: 3 Date: Fri, 21 Mar 2014 14:37:59 -0500 From: Subject: [Histonet] RE: specimen marking ink To: , Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27EFDDFC29@NADCWPMSGCMS03.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue. Don't know where it came from and don't know where it went!!! Happy Friday and Happy Weekend to everyone!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Friday, March 21, 2014 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen marking ink Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 21 Mar 2014 13:06:46 -0700 (PDT) From: Jennifer Leigh Subject: [Histonet] IHC on paraffin embedded skin tissue! To: Histonet Netserver Message-ID: <1395432406.35742.YahooMailNeo@web120703.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Histonetters- I have a question about mouse skin samples----an IHC experiment has gone wrong and I am not sure why. I have a set of paraffin embedded mouse skin samples from an inflammatory model that were stained for F4/80. The end result is no color development after the addition of DAB. I use the DAB tablets from Sigma using the TBS buffer to dissolve the tablet. There is no color development, not even non-specific staining. Any ideas what the problem might be????? I also performed a Proteinase K antigen retrevial step (using the ready made solution from Dako) for 15 minutest at 37 C as I was instructed to do. Thank you in advance for any insight! Jennifer Oskins Jennifer L. Oskins "Until one has loved an animal, part of their soul remains unawakened....." ------------------------------ Message: 5 Date: Fri, 21 Mar 2014 16:02:29 -0700 (PDT) From: David Kemler Subject: Re: [Histonet] RE: specimen marking ink To: "Wanda.Smith@HCAhealthcare.com" , "CDavis@che-east.org" , "histonet@lists.utsouthwestern.edu" Message-ID: <1395442949.1599.YahooMailNeo@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Actually, I have a tattoo supplier four miles away from me. Several years ago, I went to their warehouse to take a look an possibly make a purchase. As it turned out, I had to buy a gallon of each color and it worked out to be more than the standard tissue inks sold by the histology vendors. Next stop - tattoo shop. Same story. The artist would sell a small 8 oz. bottle at cost, but it was still just a bit more than our vendors. You can use Pelican drawing ink. It comes in several colors. It's around five something for an ounce+, much more than Davidson or one of the others. Maybe someone will also chime in. Dave On Friday, March 21, 2014 3:39 PM, "Wanda.Smith@HCAhealthcare.com" wrote: Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue. Don't know where it came from and don't know where it went!!! Happy Friday and Happy Weekend to everyone!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Friday, March 21, 2014 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specimen marking ink Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 21 Mar 2014 18:26:05 -0700 From: Bryan Llewellyn Subject: Re: [Histonet] RE: specimen marking ink To: histonet@lists.utsouthwestern.edu Message-ID: <532CE6AD.8050806@shaw.ca> Content-Type: text/plain; charset=ISO-8859-1; format=flowed You can buy tattoo ink in a bewildering array of colours from ebay quite cheaply. Many years ago I tried to buy tattoo ink from a tattoo parlour, but they refused to sell it to me as they were concerned that it would be used for rubbing into scratches and cuts for home made tattoos. I was in my mid-50's at the time. Perhaps I looked strange with my beard and long hair! I decided to buy the commercial tattoo ink kits from lab suppliers for mixed colours, but used laundry blueing for most margins and kept the tattoo inks for those occasions when it was important to know which margin was which. Bryan Llewellyn David Kemler wrote: > Actually, I have a tattoo supplier four miles away from me. Several years ago, I went to their warehouse to take a look an possibly make a purchase. As it turned out, I had to buy a gallon of each color and it worked out to be more than the standard tissue inks sold by the histology vendors. > > Next stop - tattoo shop. Same story. The artist would sell a small 8 oz. bottle at cost, but it was still just a bit more than our vendors. > > You can use Pelican drawing ink. It comes in several colors. It's around five something for an ounce+, much more than Davidson or one of the others. > > Maybe someone will also chime in. > > Dave > > > > On Friday, March 21, 2014 3:39 PM, "Wanda.Smith@HCAhealthcare.com" wrote: > > Back in the 70's (when I was six, but still a histotech!!!) my Pathologist had gotten tattoo pigment powder in two colors and that's what we used to mark tissue. Don't know where it came from and don't know where it went!!! > Happy Friday and Happy Weekend to everyone!!! > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > Sent: Friday, March 21, 2014 1:04 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] specimen marking ink > > Hi Histo World, as I was cutting to day I was thinking why don't we see if we could get specimen marking ink directly from a tattoo vendor? When I first started in histo I was told the ink we use was actually tattoo ink. As we know as soon as somebody labels something as a "medical supply" the price is increased. Just a cost saving thought, I mentioned it to my immediate supervisor but she think it would be a liability issue. I thought we could "test/validate it on skin tissue left over from a mastectomy or extremity. Any thoughts? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Fri, 21 Mar 2014 23:44:20 -0300 From: "C.D.G." Subject: Re: [Histonet] IHC on paraffin embedded skin tissue! To: Histonet@lists.utsouthwestern.edu Cc: Histonet@lists.utsouthwestern.edu Message-ID: <201403212344200234.000D0792@smtp.montevideo.com.uy> Content-Type: text/plain; charset="ISO-8859-1" Jennifer be sure to add three or four drops of H2O2 to the solution. Maybe the lack of oxidation of the chromogen is the cause of no color development. Best luck, Carlos.- > Histonetters- I have a question about mouse skin >samples----an IHC experiment has gone wrong and I am not sure why. I have >a set of paraffin embedded mouse skin samples from an inflammatory model >that were stained for F4/80. The end result is no color development after >the addition of DAB. I use the DAB tablets from Sigma using the TBS buffer >to dissolve the tablet. There is no color development, not even >non-specific staining. Any ideas what the problem might be????? I also >performed a Proteinase K antigen retrevial step (using the ready made >solution from Dako) for 15 minutest at 37 C as I was instructed to do. >Thank you in advance for any insight! Jennifer Oskins Jennifer L. >Oskins "Until one has loved an animal, part of their soul remains >unawakened....." >_______________________________________________ >Histonet mailing list > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 124, Issue 24 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Vickroy.Jim <@t> mhsil.com Mon Mar 24 16:20:16 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Mar 24 16:20:24 2014 Subject: [Histonet] Tissue Requisition Message-ID: With the constant changes in our CAP checklists and the changes in electronic ordering and moves to go paperless a question has come up that I know used to be in the checklists but I can't seem to find it now. In the past surgical pathology specimens had to be sent with a requisition. Our hospital is reviewing our process for handling specimens from various hospital departments. Currently we still require that a requisition is brought with the specimen. We have a system where the various OR staff could place an electronic order for a surgical specimen in the computer and then the paper requisition will then print in the surgical pathology grossing area. We are trying to decide if a copy of the requisition still needs to accompany the specimen when it is delivered to the grossing room. The specimens have electronic labels on them with patient identifiers and the exact site of the specimen is also listed on the label. Please tell me how others are handling these changes. Obviously I can see some advantages to no longer have illegible hand-written reqs from the OR docs or nurses, but something bothers me that a specimen will arrive without a requisition. Any thoughts? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From inno291janet <@t> yahoo.co.uk Mon Mar 24 22:17:06 2014 From: inno291janet <@t> yahoo.co.uk (Innocent Mosha) Date: Mon Mar 24 22:17:20 2014 Subject: [Histonet] Arkansas Society for Histotechnology Spring Meeting - Next Week In-Reply-To: <370637424.96928.1392924603551.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <370637424.96928.1392924603551.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <1395717426.46466.YahooMailNeo@web172301.mail.ir2.yahoo.com> Dear Histonetors! Is there anyone in his/her lab has working tissue processing machine and/or microtome and not in use anymore? Kindly do help me as I want to establish histology lab. here in Tanzania, East Africa. I will cover all costs for parking and freight! Thanking you in advance! Best Regards; Innocent J. On Thursday, 20 February 2014, 22:31, Pam Marcum wrote: The meeting starts next Friday February 28th, and Saturday March 1st. The all day Histo Prep is on Saturday. We would like to invite anyone can join us to come on and see what we have to offer. We had Shane Jones doing Histology Registry Prep all day on Saturday for anyone planning to take the test this spring. Our program includes both beginning IHC and advanced techniques. ? Robert Skinner will be talking where Orthopedic Research and treatment are going in the future with interdisciplinary cooperation in many areas of medicine. Want to know the future of mobile Histology applications for our area. Dr Shree Sharma will discuss this with a glimpse at the coming applications. Safety for the new rules in handling waste and formaldehyde in Histology will be offered with Richard Best from Stericycle. A basic Microtomy workshop is being offered to help all of us cut better sections and improve our workplace for fewer repetitive injuries. Learn the Mysteries of Histology and how to solve them. These are the highlights of our meeting with other offerings also available. If you would like more information please contact me and we send you the full registration packet with the forms, program and abstracts to review. Thank You and Join Us! Pam Marcum _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From inno291janet <@t> yahoo.co.uk Mon Mar 24 22:35:11 2014 From: inno291janet <@t> yahoo.co.uk (Innocent Mosha) Date: Mon Mar 24 22:35:16 2014 Subject: [Histonet] A help of Tissue processing machine and/or microtome! Message-ID: <1395718511.66346.YahooMailNeo@web172303.mail.ir2.yahoo.com> Dear Histonetors! I want to establish the histology lab here Tanzania, East Africa. Am looking for help/grant of any type tissue processing machine and/or microtome which are working but not in use in your lab. All cost with regarding to packing and freight I will be able to cover! Even if there extra little costs will all be covered. Kindly do help me and advice how to get those histology lab. equipment! Thanking you in advance! Best regards; Innocent Justin From Susan.Walzer <@t> HCAHealthcare.com Tue Mar 25 01:48:36 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Mar 25 01:48:43 2014 Subject: [Histonet] RE: Tissue Requisition In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FB5DA4E13@FWDCWPMSGCMS09.hca.corpad.net> Our req's come over the computer , not with specimen. We then check the req against the label on the specimen. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, March 24, 2014 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Requisition With the constant changes in our CAP checklists and the changes in electronic ordering and moves to go paperless a question has come up that I know used to be in the checklists but I can't seem to find it now. In the past surgical pathology specimens had to be sent with a requisition. Our hospital is reviewing our process for handling specimens from various hospital departments. Currently we still require that a requisition is brought with the specimen. We have a system where the various OR staff could place an electronic order for a surgical specimen in the computer and then the paper requisition will then print in the surgical pathology grossing area. We are trying to decide if a copy of the requisition still needs to accompany the specimen when it is delivered to the grossing room. The specimens have electronic labels on them with patient identifiers and the exact site of the specimen is also listed on the label. Please tell me how others are handling these changes. Obviously I can see some advantages to no longer have illegible hand-written reqs from the OR docs or nurses, but something bothers me that a specimen will arrive without a requisition. Any thoughts? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis <@t> che-east.org Tue Mar 25 08:51:07 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Tue Mar 25 08:50:48 2014 Subject: [Histonet] IHC antibody optimizing & validating Message-ID: Will you help me? I understand we are to use the known positives controls that the manufactures' recommends in the package insert when optimizing the stains, but I need to know what is your general procedure for optimizing (how many different staining protocols do you test) and validating a new antibody (how many different or "known" positive and negative tissues do you test [predictive markers I understand are 20])? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From billodonnell <@t> catholichealth.net Tue Mar 25 09:28:01 2014 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Mar 25 09:28:08 2014 Subject: [Histonet] RE: IHC antibody optimizing & validating In-Reply-To: References: Message-ID: Hi Cassie, I have tried going 'requisition free" a number of times in at least three institutions. The problems I've always encountered have been with the OR nursing staff, who frankly tell us that they are too busy to have to do "one more thing" - even if they were doing the 'thing' on paper anyway. (Now - for a little soapbox time) I have found that while there are a lot of hard working, diligent OR nurses, far too many see patient care as done once the tissue is out. This means that many times, insufficient information or even - God help us - incorrect information ends up on the requisitions. But it didn't get any better electronically, in fact it was worse. Having required fields before submission helps - but only if they bother to put something useful in them. This may not be everyone's experience, in fact I hope it is not. My problem is not with paperless (which is never really paperless) but with trusting the people who do the ordering. Any way, thanks for letting me have a mini-rant. It felt good! Shalom - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, March 25, 2014 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC antibody optimizing & validating Will you help me? I understand we are to use the known positives controls that the manufactures' recommends in the package insert when optimizing the stains, but I need to know what is your general procedure for optimizing (how many different staining protocols do you test) and validating a new antibody (how many different or "known" positive and negative tissues do you test [predictive markers I understand are 20])? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From tpodawiltz <@t> lrgh.org Tue Mar 25 09:54:48 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Mar 25 09:54:53 2014 Subject: [Histonet] RE: IHC antibody optimizing & validating In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D9863863255AAD9A47@LRGHEXVS1.practice.lrgh.org> Don't even get me going. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, March 25, 2014 10:28 AM To: Davis, Cassie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC antibody optimizing & validating Hi Cassie, I have tried going 'requisition free" a number of times in at least three institutions. The problems I've always encountered have been with the OR nursing staff, who frankly tell us that they are too busy to have to do "one more thing" - even if they were doing the 'thing' on paper anyway. (Now - for a little soapbox time) I have found that while there are a lot of hard working, diligent OR nurses, far too many see patient care as done once the tissue is out. This means that many times, insufficient information or even - God help us - incorrect information ends up on the requisitions. But it didn't get any better electronically, in fact it was worse. Having required fields before submission helps - but only if they bother to put something useful in them. This may not be everyone's experience, in fact I hope it is not. My problem is not with paperless (which is never really paperless) but with trusting the people who do the ordering. Any way, thanks for letting me have a mini-rant. It felt good! Shalom - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, March 25, 2014 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC antibody optimizing & validating Will you help me? I understand we are to use the known positives controls that the manufactures' recommends in the package insert when optimizing the stains, but I need to know what is your general procedure for optimizing (how many different staining protocols do you test) and validating a new antibody (how many different or "known" positive and negative tissues do you test [predictive markers I understand are 20])? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From joelleweaver <@t> hotmail.com Tue Mar 25 09:58:15 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Mar 25 09:58:19 2014 Subject: [Histonet] IHC antibody optimizing & validating In-Reply-To: References: Message-ID: I think that there is some variability to the methodology in this, and also depends on the constraints of your staining platform or if doing manual IHC. Below are my current habits, working alone and performing multiple validations at the same time...others may have their own way of accomplishing. The big thing to me is to keep excellent records for CAP. My opinion is, that the number of slides or different protocols needed to optimize is the number of slides or trials it takes to get the desired target staining versus high background, non-specific staining, other variables - until you are satisfied you have the desired staining of the specific tissue element, antigen, protein bacteria....etc; that the antibody targets. So I am not sure there is a specific required number, but if you do one for each retrieval stringency in your platforms ( automated), and then change the incubation times if you can, and maybe the AB titer a couple of trials, that is at least 4-8 slides, maybe more or less depending on your system. Sometimes you can hit it right off working from the specification sheet, but sometimes not. Usually after a couple of tests though, you can see if you are getting there or which direction to proceed. For setting up a completely new protocol with unfamiliar antibody, I use an expected positive, sometimes normal, sometimes tumor section ( depending on the antibody) same tissue at this point, that I know to be well fixed and processed, and change one of the staining variables after each attempt( tissue stays constant) sticking pretty close to the specification sheet if IVD. If none of the usual tweaks work, I try different tissue and go back into it again. I document the changes and the results each time so I don't lose track of things on my worksheets.If you are doing an IVD, then the specification sheet is the way to go with how to start and follow the recommendations and tweak for your lab conditions- changing one variable at a time until you are satisfied with the result. Usually the optimization and best slide/protocol is determined in consultation with a pathologist. Once the stain is optimized, I do parallel runs of known positives and negatives ( usually at least 10 cases for IVD ( which may be 25 slides or more if needed) and more for ASR antibodies). Until you have enough to establish the stain is working consistently across multiple tissue samples, best to stress the tissue type you expect to use the stain on in your testing and to use your own fixation, processing and slide handling that you will again do with patient tissues in your parallel/validation studies. The pathologist or medical director will review all the validation slides and either accept and sign off on the test, or send you back to the drawing board. For ASR, I consult literature to start, but there isn't much in the specification sheets as far as exact of manufacturer's recommendations. So this relies more on experience and close review of results from optimization slides and tweaking from there. For those, I personally do at least 25 cases. For predictive markers I do 20 negative (have tumor-but non amplified/-) and 20 positive ( tumor and +/amplified) -so at least forty slides/tissue samples minimum for predictive markers, then you do the correlation. Keep everything for CAP. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: CDavis@che-east.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Mar 2014 09:51:07 -0400 > Subject: [Histonet] IHC antibody optimizing & validating > > Will you help me? I understand we are to use the known positives controls that the manufactures' recommends in the package insert when optimizing the stains, but I need to know what is your general procedure for optimizing (how many different staining protocols do you test) and validating a new antibody (how many different or "known" positive and negative tissues do you test [predictive markers I understand are 20])? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mburns <@t> atlanticurologyclinics.com Tue Mar 25 10:08:06 2014 From: mburns <@t> atlanticurologyclinics.com (Melissa Burns) Date: Tue Mar 25 10:08:13 2014 Subject: [Histonet] Biocare Uroplakin II Message-ID: <8F26C28A6B397C47BFC334F7B2D6FD9815FFA0A2@AUC-Exchange1.gsuro.com> Is anyone currently using the Biocare Uroplakin II IHC? How do your pathologists's like it? We are thinking of trying it out. We have the Ventana XT instrument, so if you happen to have your protocol and are willing to share, that would be awesome! Thanks! Melissa L. Burns Pathology Lab Manager, Histotechnician From flnails <@t> texaschildrens.org Tue Mar 25 10:11:45 2014 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Mar 25 10:11:51 2014 Subject: [Histonet] Req Storage Message-ID: <327E034F1892504289B7A17EC71DF9F3093961@TCFMSG03.ad.texaschildrenshospital.org> Has anyone started scanning requisitions and discarding the original AP reqs? If so does this meet the CAP retention requirement? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From lpwenk <@t> sbcglobal.net Tue Mar 25 11:10:24 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Mar 25 11:10:28 2014 Subject: [Histonet] PT Histotech needed in NJ Message-ID: Posting this for a friend, so please reply to him, and not to me. Part-time ASCP certified histotech needed in private derm/GI lab in New Jersey. Day shift Cut 40-50 blocks/day Typical special stains include Steiner, Alcian Blue-PAS, and GMS Contact John Howard at 973-650-4038. Peggy A. Wenk, HTL(ASCP) From Bruce_Palmatier <@t> vwr.com Tue Mar 25 13:00:38 2014 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Tue Mar 25 13:01:08 2014 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 03/27/2014) Message-ID: I am out of the office until 03/27/2014. I will be out of the office from March 25th until March 27th. I will be checking emails periodically and will attempt to reply to your message within 24 hours. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 124, Issue 26" sent on 3/25/2014 11:11:10 AM. This is the only notification you will receive while this person is away. From philip_manfre <@t> merck.com Tue Mar 25 13:27:52 2014 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Tue Mar 25 13:27:59 2014 Subject: [Histonet] Paraffin Effects on IHC Message-ID: <558A4571351D0C42BD923F403F4198C4C36F9C4D12@USCTMXP51014.merck.com> Our lab is considering switching paraffin in our histology labs. They are being evaluated with an emphasis on routine H&E staining. The IHC lab has some concerns about the effects this may have on IHC staining. We have noticed slight differences in staining intensity already, during some evaluation experiments. Does anyone have opinions either way, positive or negative, on the following products? Of particular emphasis are any effects on the staining intensity (which also could relate to the ease of deparaffinization of the sections), ability to stain evenly, or anything else you may feel is relevant. 1. Fisher Scientific TissuePrep T-565 2. Leica Paraplast 3. ThermoScientific Histoplast Thanks all for your expertise! It's great that we have such a global forum for our field. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From liz <@t> premierlab.com Tue Mar 25 13:41:49 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Mar 25 13:41:54 2014 Subject: [Histonet] RE: Paraffin Effects on IHC In-Reply-To: <558A4571351D0C42BD923F403F4198C4C36F9C4D12@USCTMXP51014.merck.com> References: <558A4571351D0C42BD923F403F4198C4C36F9C4D12@USCTMXP51014.merck.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E056F6@SBS2K8.premierlab.local> Phil Are the slides prepared from the different paraffins being testing run on the same H&E stain run or on different runs? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Manfre, Philip Sent: Tuesday, March 25, 2014 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Effects on IHC Our lab is considering switching paraffin in our histology labs. They are being evaluated with an emphasis on routine H&E staining. The IHC lab has some concerns about the effects this may have on IHC staining. We have noticed slight differences in staining intensity already, during some evaluation experiments. Does anyone have opinions either way, positive or negative, on the following products? Of particular emphasis are any effects on the staining intensity (which also could relate to the ease of deparaffinization of the sections), ability to stain evenly, or anything else you may feel is relevant. 1. Fisher Scientific TissuePrep T-565 2. Leica Paraplast 3. ThermoScientific Histoplast Thanks all for your expertise! It's great that we have such a global forum for our field. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Tue Mar 25 14:34:30 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Mar 25 14:34:41 2014 Subject: [Histonet] RE: IHC antibody optimizing & validating In-Reply-To: <20140325162111.E40F31E806E@trendmess-svr.holyredeemer.local> References: <20140325162111.E40F31E806E@trendmess-svr.holyredeemer.local> Message-ID: CAP is very clear that in order to validate a new antibody, that: "once the stain has been optimized, that for a well characterized antibody with a limited spectrum of antigenic targets, like Chromogranin or PSA, the validation can be limited. A panel of 10 positive and 10 negative neoplasms would be sufficient in this setting. For an antibody that is not well characterized and/or has a wide range of reported reactivity, a more extensive validation is necessary. The number of tissues tested should in the circumstance be large enough to determine whether the staining profile matches that previously described. An exception to the above requirements is that studies nay not be feasible for antigens such as ALK that are only seen in rare tumors." Thus sayeth CAP. And if you're like me, I am not digging through all my cases to try to come up with 30-40 neoplasms for each antibody, so I just order Tissue Micro Arrays with the neoplasms I need. 20 positive and 20 negative neoplastic tissues on one slide for easy staining and validation. The money you save on reagents to stain one little slide, more than makes up for the cost of the slide. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 8. IHC antibody optimizing & validating (Davis, Cassie) Message: 8 Date: Tue, 25 Mar 2014 09:51:07 -0400 From: "Davis, Cassie" Subject: [Histonet] IHC antibody optimizing & validating Will you help me? I understand we are to use the known positives controls that the manufactures' recommends in the package insert when optimizing the stains, but I need to know what is your general procedure for optimizing (how many different staining protocols do you test) and validating a new antibody (how many different or "known" positive and negative tissues do you test [predictive markers I understand are 20])? Cassandra Davis CDavis@che-east.org 302-575-8095 ********************************* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From joelleweaver <@t> hotmail.com Tue Mar 25 14:45:30 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Mar 25 14:45:44 2014 Subject: [Histonet] RE: IHC antibody optimizing & validating In-Reply-To: References: <20140325162111.E40F31E806E@trendmess-svr.holyredeemer.local>, Message-ID: TMAs are great if that is one of your options, but unless you make them you have the PA issues that might enter in. CAP does gives specifics, but I was just giving the information and process that is currently in place where I presently work to hopefully help, not trying to supersede CAP guidelines for sure- where would that land me? Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 25 Mar 2014 15:34:30 -0400 > From: tbraud@holyredeemer.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: IHC antibody optimizing & validating > > CAP is very clear that in order to validate a new antibody, that: "once > the stain has been optimized, that for a well characterized antibody > with a limited spectrum of antigenic targets, like Chromogranin or PSA, > the validation can be limited. A panel of 10 positive and 10 negative > neoplasms would be sufficient in this setting. For an antibody that is > not well characterized and/or has a wide range of reported reactivity, a > more extensive validation is necessary. The number of tissues tested > should in the circumstance be large enough to determine whether the > staining profile matches that previously described. An exception to the > above requirements is that studies nay not be feasible for antigens such > as ALK that are only seen in rare tumors." > Thus sayeth CAP. > And if you're like me, I am not digging through all my cases to try to > come up with 30-40 neoplasms for each antibody, so I just order Tissue > Micro Arrays with the neoplasms I need. 20 positive and 20 negative > neoplastic tissues on one slide for easy staining and validation. The > money you save on reagents to stain one little slide, more than makes up > for the cost of the slide. > I hope this helps. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > Today's Topics: > 8. IHC antibody optimizing & validating (Davis, Cassie) > > Message: 8 > Date: Tue, 25 Mar 2014 09:51:07 -0400 > From: "Davis, Cassie" > Subject: [Histonet] IHC antibody optimizing & validating > > Will you help me? I understand we are to use the known positives > controls that the manufactures' recommends in the package insert when > optimizing the stains, but I need to know what is your general procedure > for optimizing (how many different staining protocols do you test) and > validating a new antibody (how many different or "known" positive and > negative tissues do you test [predictive markers I understand are 20])? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > ********************************* > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it was sent. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Tue Mar 25 14:55:39 2014 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Mar 25 14:55:45 2014 Subject: [Histonet] RE: Req Storage In-Reply-To: <327E034F1892504289B7A17EC71DF9F3093961@TCFMSG03.ad.texaschildrenshospital.org> References: <327E034F1892504289B7A17EC71DF9F3093961@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: I'm guessing the procedure you discribe does, as we have been doing it that way for years. We keep our hard copies on site for a week before sending them to be scanned. Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Nails, Felton [flnails@texaschildrens.org] Sent: Tuesday, March 25, 2014 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Req Storage Has anyone started scanning requisitions and discarding the original AP reqs? If so does this meet the CAP retention requirement? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From litepath2000 <@t> yahoo.com Tue Mar 25 15:02:19 2014 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Tue Mar 25 15:02:22 2014 Subject: [Histonet] Labvision Contact Info Message-ID: <1395777739.56044.YahooMailNeo@web140302.mail.bf1.yahoo.com> Anyone who can help out..... I am trying to track down anyone from Labvision (now thermo) who was part of the group that help set-up domains and hosting for all the state society webpages back in 2004.? Please contact me off list Thanks L ? ------- Luis Chiriboga Ph.D. President, New York State Histotechnological Society NYSHS Website: www.nyhisto.org NYSHS Message Board: http://tech.groups.yahoo.com/group/NYSHS1972/ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. From norcalmech <@t> gmail.com Tue Mar 25 15:35:22 2014 From: norcalmech <@t> gmail.com (Victor Gravley) Date: Tue Mar 25 15:37:05 2014 Subject: [Histonet] (no subject) Message-ID: Please remove us from the mailing list Sent from my iPhone From trathborne <@t> somerset-healthcare.com Tue Mar 25 15:43:04 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 25 15:43:34 2014 Subject: [Histonet] RE: Tissue Requisition In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FB5DA4E13@FWDCWPMSGCMS09.hca.corpad.net> References: <4BF03F5404EBDE409AF9232DA74B9DED2FB5DA4E13@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95C1D97@SMCMAIL01.somerset-healthcare.com> Our experiences have not been that bad with regard to electronic ordering. We still do get the paper requisitions with the specimens, but they are bar code scanned when accessioned in the grossing area. We got a lot of help from our IT department, through a joint effort with the OR and other submitting areas, and by hospital wide initiatives. We even have order sets for breast and colon. This makes it easier for the RN placing the order by going to a drop-down and selecting the correct site. There has been talk of going paperless, but it's a big step. Although we haven't received an unlabeled specimen in years, there is always that fear that a specimen will arrive without one. At least with the attached requisition one can be somewhat certain that they belong together if everything else matches. For specimens like GIs it might be hard to tell one from another, although unlikely that two would arrive unlabeled, there is still that reluctance to go paperless. I would check with your LIS vendor, and get a list of users that do order electronically. Talk to them and find out if there was resistance to make the change, and if so how it was overcome. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Tuesday, March 25, 2014 2:49 AM To: Vickroy.Jim@mhsil.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue Requisition Our req's come over the computer , not with specimen. We then check the req against the label on the specimen. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, March 24, 2014 5:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Requisition With the constant changes in our CAP checklists and the changes in electronic ordering and moves to go paperless a question has come up that I know used to be in the checklists but I can't seem to find it now. In the past surgical pathology specimens had to be sent with a requisition. Our hospital is reviewing our process for handling specimens from various hospital departments. Currently we still require that a requisition is brought with the specimen. We have a system where the various OR staff could place an electronic order for a surgical specimen in the computer and then the paper requisition will then print in the surgical pathology grossing area. We are trying to decide if a copy of the requisition still needs to accompany the specimen when it is delivered to the grossing room. The specimens have electronic labels on them with patient identifiers and the exact site of the specimen is also listed on the label. Please tell me how others are handling these changes. Obviously I can see some advantages to no longer have illegible hand-written reqs from the OR docs or nurses, but something bothers me that a specimen will arrive without a requisition. Any thoughts? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rlhenshall_powell <@t> yahoo.co.uk Tue Mar 25 23:52:47 2014 From: rlhenshall_powell <@t> yahoo.co.uk (Rhonda Henshall-Powell) Date: Tue Mar 25 23:52:58 2014 Subject: [Histonet] Open Technical Support Specialist position at Northern CA IHC Company Message-ID: <1395809567.36021.YahooMailNeo@web172501.mail.ir2.yahoo.com> Hello, I am currently seeking applications for an open Technical Support Specialist position at a well known Northern Californian Cancer Diagnostics IHC company. The candidate will be based in the local vicinity to the corporate headquarters and will be expected to provide Technical/Applications support to customers and employees on all product related issues via telephone, web and email communications. ?The candidate should preferably have histology laboratory experience and be prepared to troubleshoot manual and automated IHC procedures. Please email me privately with your inquiries and resume if interested. Best Regards, Rhonda Henshall-Powell rlhenshall_powell@yahoo.co.uk From leila.etemadi <@t> med.lu.se Wed Mar 26 01:24:08 2014 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Wed Mar 26 01:24:18 2014 Subject: [Histonet] Rabbit anti-Galanin (human) Message-ID: Hi, I wonder if any one out there working with Peninsula Rabbit anti-Galanin (human)?, I am planing to use it on the rat nervous system tissue which is new work for my lab. I will be appreciate if you can share your great protocol with me :-) Cheers, Leila From Richard.Cartun <@t> hhchealth.org Wed Mar 26 10:47:26 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Mar 26 10:47:39 2014 Subject: [Histonet] RE: IHC antibody optimizing & validating In-Reply-To: References: <20140325162111.E40F31E806E@trendmess-svr.holyredeemer.local> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0229871E@HHCEXCHMB03.hhcsystem.org> I have not read the entire document yet. What do they say about using tissues that have been fixed and processed elsewhere? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, March 25, 2014 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC antibody optimizing & validating CAP is very clear that in order to validate a new antibody, that: "once the stain has been optimized, that for a well characterized antibody with a limited spectrum of antigenic targets, like Chromogranin or PSA, the validation can be limited. A panel of 10 positive and 10 negative neoplasms would be sufficient in this setting. For an antibody that is not well characterized and/or has a wide range of reported reactivity, a more extensive validation is necessary. The number of tissues tested should in the circumstance be large enough to determine whether the staining profile matches that previously described. An exception to the above requirements is that studies nay not be feasible for antigens such as ALK that are only seen in rare tumors." Thus sayeth CAP. And if you're like me, I am not digging through all my cases to try to come up with 30-40 neoplasms for each antibody, so I just order Tissue Micro Arrays with the neoplasms I need. 20 positive and 20 negative neoplastic tissues on one slide for easy staining and validation. The money you save on reagents to stain one little slide, more than makes up for the cost of the slide. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 8. IHC antibody optimizing & validating (Davis, Cassie) Message: 8 Date: Tue, 25 Mar 2014 09:51:07 -0400 From: "Davis, Cassie" Subject: [Histonet] IHC antibody optimizing & validating Will you help me? I understand we are to use the known positives controls that the manufactures' recommends in the package insert when optimizing the stains, but I need to know what is your general procedure for optimizing (how many different staining protocols do you test) and validating a new antibody (how many different or "known" positive and negative tissues do you test [predictive markers I understand are 20])? Cassandra Davis CDavis@che-east.org 302-575-8095 ********************************* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tbraud <@t> holyredeemer.com Wed Mar 26 11:48:46 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Mar 26 11:48:49 2014 Subject: [Histonet] RE: IHC antibody optimizing & validating In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E0229871E@HHCEXCHMB03.hhcsystem.org> References: <20140325162111.E40F31E806E@trendmess-svr.holyredeemer.local> <9215BD4B0BA1B44D962A71C758B68D2E0229871E@HHCEXCHMB03.hhcsystem.org> Message-ID: Fortunately, they say nothing at all because if that were the case, they would no longer be able to peddle their Proficiency Programs for IHC, since those too, are fixed and processed elsewhere. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun@hhchealth.org] Sent: Wednesday, March 26, 2014 11:47 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: IHC antibody optimizing & validating I have not read the entire document yet. What do they say about using tissues that have been fixed and processed elsewhere? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, March 25, 2014 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC antibody optimizing & validating CAP is very clear that in order to validate a new antibody, that: "once the stain has been optimized, that for a well characterized antibody with a limited spectrum of antigenic targets, like Chromogranin or PSA, the validation can be limited. A panel of 10 positive and 10 negative neoplasms would be sufficient in this setting. For an antibody that is not well characterized and/or has a wide range of reported reactivity, a more extensive validation is necessary. The number of tissues tested should in the circumstance be large enough to determine whether the staining profile matches that previously described. An exception to the above requirements is that studies nay not be feasible for antigens such as ALK that are only seen in rare tumors." Thus sayeth CAP. And if you're like me, I am not digging through all my cases to try to come up with 30-40 neoplasms for each antibody, so I just order Tissue Micro Arrays with the neoplasms I need. 20 positive and 20 negative neoplastic tissues on one slide for easy staining and validation. The money you save on reagents to stain one little slide, more than makes up for the cost of the slide. I hope this helps. Terri L. Braud, HT(ASCP) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From joelleweaver <@t> hotmail.com Wed Mar 26 11:59:23 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Mar 26 11:59:27 2014 Subject: [Histonet] RE: IHC antibody optimizing & validating In-Reply-To: References: <20140325162111.E40F31E806E@trendmess-svr.holyredeemer.local>, , <9215BD4B0BA1B44D962A71C758B68D2E0229871E@HHCEXCHMB03.hhcsystem.org>, Message-ID: only 1/2 way through reading the article, but Terri makes a good point about the proficiency testing. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 26 Mar 2014 12:48:46 -0400 > From: tbraud@holyredeemer.com > To: Richard.Cartun@hhchealth.org; histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] RE: IHC antibody optimizing & validating > > Fortunately, they say nothing at all because if that were the case, they > would no longer be able to peddle their Proficiency Programs for IHC, > since those too, are fixed and processed elsewhere. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > > -----Original Message----- > From: Cartun, Richard [mailto:Richard.Cartun@hhchealth.org] > Sent: Wednesday, March 26, 2014 11:47 AM > To: Terri Braud; histonet@lists.utsouthwestern.edu > Subject: RE: IHC antibody optimizing & validating > > I have not read the entire document yet. What do they say about using > tissues that have been fixed and processed elsewhere? > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri > Braud > Sent: Tuesday, March 25, 2014 3:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: IHC antibody optimizing & validating > > CAP is very clear that in order to validate a new antibody, that: "once > the stain has been optimized, that for a well characterized antibody > with a limited spectrum of antigenic targets, like Chromogranin or PSA, > the validation can be limited. A panel of 10 positive and 10 negative > neoplasms would be sufficient in this setting. For an antibody that is > not well characterized and/or has a wide range of reported reactivity, a > more extensive validation is necessary. The number of tissues tested > should in the circumstance be large enough to determine whether the > staining profile matches that previously described. An exception to the > above requirements is that studies nay not be feasible for antigens such > as ALK that are only seen in rare tumors." > Thus sayeth CAP. > And if you're like me, I am not digging through all my cases to try to > come up with 30-40 neoplasms for each antibody, so I just order Tissue > Micro Arrays with the neoplasms I need. 20 positive and 20 negative > neoplastic tissues on one slide for easy staining and validation. The > money you save on reagents to stain one little slide, more than makes up > for the cost of the slide. > I hope this helps. > > Terri L. Braud, HT(ASCP) > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it was sent. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Wed Mar 26 12:24:23 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Mar 26 12:28:03 2014 Subject: [Histonet] CAP Question, New Reagent Lot Confirmation of Acceptability Message-ID: <12ECD7346266D74691EC2BFC75285E452F44DFCD@BFL323E10.pathmdlabs.local> The above checklist item, ANP.22760, states "The performance of new lots of antibody and detection system reagents is compared with old lots before or concurrently with being placed into service." Neither the question, the note below it, nor the evidence of compliance states that a pathologist has to perform the comparison. What are other labs doing? Are the histotechs signing off on new lots? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From b427297 <@t> aol.com Wed Mar 26 15:00:16 2014 From: b427297 <@t> aol.com (William J. O'Connor III) Date: Wed Mar 26 15:00:23 2014 Subject: [Histonet] Frozen sectioning whole rat eyes In-Reply-To: References: <20140325162111.E40F31E806E@trendmess-svr.holyredeemer.local>, , <9215BD4B0BA1B44D962A71C758B68D2E0229871E@HHCEXCHMB03.hhcsystem.org>, Message-ID: <8D11745242064F2-2240-3ABE@webmail-vm061.sysops.aol.com> Hello Histoland! A colleague of mine is trying to section unfixed whole rat eyes in OCT. She is getting a lot of tearing at the top of her section. Lens and optic nerve on the horizontal plane. Any suggestions, recommendations would be great! Jackie From LRaff <@t> uropartners.com Wed Mar 26 15:03:27 2014 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Wed Mar 26 15:03:39 2014 Subject: [Histonet] IHC on animals Message-ID: Hi: Can IHC validated for humans be performed on animal tissues without any protocol changes? Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From shive003 <@t> umn.edu Wed Mar 26 15:11:18 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Mar 26 15:11:33 2014 Subject: [Histonet] IHC on animals In-Reply-To: References: Message-ID: Dr. Raff, You will need to do trial runs to first determine if the antibody can detect the specific epitope in animal tissues (epitopes aren't always conserved between species), and then do your own optimization to determine the best dilution and tissue pretreatment for that particular species. They can vary by species, and don't always coincide with the recommendations on the antibody data sheet provided by the vendor. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu On Wed, Mar 26, 2014 at 3:03 PM, Lester Raff MD wrote: > Hi: > > > > Can IHC validated for humans be performed on animal tissues without any > protocol changes? > > > > Thanks, > > > > Lester J. Raff, MD MBA > > UroPartners > > Medical Director Of Laboratory > > 2225 Enterprise Dr. Suite 2511 > > Westchester, Il 60154 > > Tel: 708-486-0076 > > Fax: 708-492-0203 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Wed Mar 26 15:19:48 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Mar 26 15:19:53 2014 Subject: [Histonet] RE: IHC on animals In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05725@SBS2K8.premierlab.local> Lester I would not, first of all not all antibodies that work in human will cross react with a particular species of animal. Another thing to consider is that most human based detection systems consist of dual link reagents, meaning that they work on both mouse and rabbit primaries these detection system are not ideal for working with animals and depending upon the species that you are working with may cause some problems in with background staining due to the secondary antibody or polymer binding to endogenous IgG in the samples. You can initially pilot your protocol on a particular species to see what happens but I would not take the chance and run a bunch of slides thinking that the protocol that you have in place will work in the particular species of animal you need to stain. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Wednesday, March 26, 2014 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on animals Hi: Can IHC validated for humans be performed on animal tissues without any protocol changes? Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Allison.Scott <@t> harrishealth.org Wed Mar 26 16:10:58 2014 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Wed Mar 26 16:11:06 2014 Subject: [Histonet] Body Release and Cleanin in Morgue Message-ID: Hello to all in histo-land. Who takes care of releasing the body to the funeral home and who does the cleaning of the seals and the molds on the drawers for the body storage? Is lab some how involved in this process? Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From pruegg <@t> ihctech.net Wed Mar 26 16:55:31 2014 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Wed Mar 26 16:55:33 2014 Subject: [Histonet] RE: IHC on animals In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E05725@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79E05725@SBS2K8.premierlab.local> Message-ID: <004201cf493e$1c771540$55653fc0$@ihctech.net> Lester, Probably not in most cases but it depends upon what the antibody is, whether as a an antihuman ab it also cross reacts to the species you are interested in and what detection reagents you are using. For instance if you have determined a rabbit anti human antibody also cross reacts in mouse tissue, you could use a detection such as for the Leica Bond by leaving out the link (they call it the post primary but it is rabbit anti mouse IgG to link mouse antibodies to the goat anti rabbit labeled polymer second step), since your ab is rabbit it would link directly to the anti rab labeled polymer and the anti mouse link which would bind non specifically to the mouse endogenous Ig can be avoided. You would have to do a lot of research to first determine if the anti human rabbit antibody you want to use (it should not be made in a mouse) cross reacts to mouse tissue. The species you are wanting to label is also very important, this system would only work for anti human rab abs that also react in mouse, rat or any other species except rabbit and goat because those are the species the detection is made in or against. We have research versions of the Leica Bond instruments which allows us to manipulate the instrument and detection for these purposes including replacing the rab anti ms link with another link, I am not sure if the pure clinical versions of the Bond allow this. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, March 26, 2014 2:20 PM To: Lester Raff MD; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC on animals Lester I would not, first of all not all antibodies that work in human will cross react with a particular species of animal. Another thing to consider is that most human based detection systems consist of dual link reagents, meaning that they work on both mouse and rabbit primaries these detection system are not ideal for working with animals and depending upon the species that you are working with may cause some problems in with background staining due to the secondary antibody or polymer binding to endogenous IgG in the samples. You can initially pilot your protocol on a particular species to see what happens but I would not take the chance and run a bunch of slides thinking that the protocol that you have in place will work in the particular species of animal you need to stain. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Wednesday, March 26, 2014 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on animals Hi: Can IHC validated for humans be performed on animal tissues without any protocol changes? Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TanyaAbbott <@t> catholichealth.net Thu Mar 27 06:42:43 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Thu Mar 27 06:42:48 2014 Subject: [Histonet] H. pylori Message-ID: <852F7D2C14FB464D80E182B15DB138AF30661C6F@CHIEX005.CHI.catholichealth.net> Can anyone recommend a good commercially bought slide for H.pylori? Thanks! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From trathborne <@t> somerset-healthcare.com Thu Mar 27 07:46:44 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 27 07:47:13 2014 Subject: [Histonet] RE: Body Release and Cleanin in Morgue In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95C3549@SMCMAIL01.somerset-healthcare.com> Security is responsible for the release of bodies to funeral homes, and all staff must contact Security to access the morgue. We have found that to be the best way to avoid being questioned about anything. We have stretchers here not drawers, so I can't respond to that. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, March 26, 2014 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Body Release and Cleanin in Morgue Hello to all in histo-land. Who takes care of releasing the body to the funeral home and who does the cleaning of the seals and the molds on the drawers for the body storage? Is lab some how involved in this process? Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Thu Mar 27 08:16:29 2014 From: sbaldwin <@t> mhhcc.org (Sara Baldwin/mhhcc.org) Date: Thu Mar 27 08:16:38 2014 Subject: [Histonet] IHC VALIDATION Message-ID: Histonetters does anyone have a validation form that reflects the new principles of validation out there that would be willing to share??? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 "Christ's healing mission of compassion empowers us to be for others through quality and excellence." From Richard.Cartun <@t> hhchealth.org Thu Mar 27 09:06:29 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Mar 27 09:06:37 2014 Subject: [Histonet] IHC VALIDATION Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E022989E8@HHCEXCHMB03.hhcsystem.org> I keep an Excel spreadsheet on all my antibodies (and probes). I list the case number, the tissue tested, the result, "is this the expected result?", diagnosis and/or comments, and the detection system used. I am a firm believer of maintaining a "prospective" validation meaning I add positive and negative cases to the spreadsheet even after the primary validation is completed. This allows you to monitor for analytical drift, and also proves useful in identifying cases for educational purposes and for identifying control material. I will send you (and anyone else who is interested) a copy of the blank template. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org Sent: Thursday, March 27, 2014 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC VALIDATION Histonetters does anyone have a validation form that reflects the new principles of validation out there that would be willing to share??? Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 "Christ's healing mission of compassion empowers us to be for others through quality and excellence." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From CDavis <@t> che-east.org Thu Mar 27 09:31:13 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu Mar 27 09:33:08 2014 Subject: [Histonet] MUM-1 is the word. In-Reply-To: References: , Message-ID: Patrick, Kudos to you, you saved my sanity. It came out B-E-U-tiful. I just hope our Medical Direct likes it. Thank you, thank you, thank you! Cassandra Davis CDavis@che-east.org 302-575-8095 ________________________________ From: Patrick Laurie [foreightl@gmail.com] Sent: Thursday, March 13, 2014 11:03 AM To: Davis, Cassie Subject: Re: [Histonet] MUM-1 Hi Cassandra, We run it at our lab, and our incredibly picky Hemepath is happy. But our protocol is unusual. We run extended CC1 and a 4 minute antibody incubation. We are using a large B cell lymphoma for our QC, which is working very well. If you need a QC block, we have several, I would be happy to send you one. Thanks, Patrick Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Thu, Mar 13, 2014 at 9:53 AM, Davis, Cassie > wrote: Good morning Histonet Folks, I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the "usual adjustments" and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this. Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Wanda.Smith <@t> HCAhealthcare.com Thu Mar 27 10:59:31 2014 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Mar 27 10:59:43 2014 Subject: [Histonet] RE: Body Release and Cleanin in Morgue In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27F01307C2@NADCWPMSGCMS03.hca.corpad.net> It is Nursing Services at my Hospital. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, March 26, 2014 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Body Release and Cleanin in Morgue Hello to all in histo-land. Who takes care of releasing the body to the funeral home and who does the cleaning of the seals and the molds on the drawers for the body storage? Is lab some how involved in this process? Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terri.Brown <@t> Northside.com Thu Mar 27 11:11:26 2014 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Thu Mar 27 11:12:22 2014 Subject: [Histonet] RE: Body Release and Cleanin in Morgue Message-ID: <731941C266951A47BEF11E5EFAAED9C91EF5DA9F@nsmvexch01.northside.local> It is the Pathology Lab at Northside Atlanta for releasing a body to funeral home. We have engineering to clean seals on our cooler and EVS to mop in the cooler weekly. Terri H. Brown, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Thursday, March 27, 2014 12:00 PM To: Allison.Scott@harrishealth.org; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Body Release and Cleanin in Morgue It is Nursing Services at my Hospital. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, March 26, 2014 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Body Release and Cleanin in Morgue Hello to all in histo-land. Who takes care of releasing the body to the funeral home and who does the cleaning of the seals and the molds on the drawers for the body storage? Is lab some how involved in this process? Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From TMcNemar <@t> lmhealth.org Thu Mar 27 11:45:37 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Mar 27 11:45:48 2014 Subject: [Histonet] RE: Body Release and Cleanin in Morgue In-Reply-To: References: Message-ID: We stopped doing autopsies years ago. Since then, we have transferred ownership of the morgue to Nursing Services and have nothing to do with it. Before all that it was the deiner who was responsible for releasing the bodies and saw that it was cleaned by environmental services after each autopsy. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, March 26, 2014 5:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Body Release and Cleanin in Morgue Hello to all in histo-land. Who takes care of releasing the body to the funeral home and who does the cleaning of the seals and the molds on the drawers for the body storage? Is lab some how involved in this process? Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From sforeman <@t> labpath.com Thu Mar 27 11:45:16 2014 From: sforeman <@t> labpath.com (Susan Foreman) Date: Thu Mar 27 11:51:35 2014 Subject: [Histonet] IHC blast cells in bone marrow specimen Message-ID: <003d01cf49db$ee8bf990$cba3ecb0$@com> Good afternoon, Histonetters. What antibody do you have good luck with for staining blast cells in bone marrow specimen (ffpe). By the way, we are using the Ventana Benchmark Ultra staining platform. Our pathologist isn't super enthused about our current antibody & clone. Many Thanks, Susan From CDavis <@t> che-east.org Thu Mar 27 13:09:17 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu Mar 27 13:08:56 2014 Subject: [Histonet] RE: CAP Question, New Reagent Lot Confirmation of Message-ID: A pathologist is designated to sign off in our lab. Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From pruegg <@t> ihctech.net Thu Mar 27 13:22:28 2014 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Thu Mar 27 13:22:29 2014 Subject: [Histonet] processing tissue with silicone mesh in it Message-ID: <007101cf49e9$8326a7f0$8973f7d0$@ihctech.net> Does anyone have any experience with processing tissues with silicone mesh in them so that the silicone is not dissolved by xylene and we also assume that it might be dissolved by MMA or GMA polymers since they are strong solvents in their own right? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com From saby_joseph_a <@t> yahoo.com Thu Mar 27 13:54:16 2014 From: saby_joseph_a <@t> yahoo.com (saby_joseph_a) Date: Thu Mar 27 13:54:30 2014 Subject: [Histonet] processing tissue with silicone mesh in it Message-ID: Patsy- We routinely process and section many meshes in paraffin. I would suggest processing a sample, embedding and sectioning to see if you can get good sections. It is worth a try. Joe Saby NAMSA Sent on the new Sprint Network from my Samsung Galaxy S?4. -------- Original message -------- From: pruegg@ihctech.net Date:03/27/2014 2:22 PM (GMT-05:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing tissue with silicone mesh in it Does anyone have any experience with processing tissues with silicone mesh in them so that the silicone is not dissolved by xylene and we also assume that it might be dissolved by MMA or GMA polymers since they are strong solvents in their own right? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Mar 27 18:16:13 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Mar 27 18:16:18 2014 Subject: [Histonet] monkey question Message-ID: <3A3AF72F-B27A-44D2-B148-BC46A166EACB@email.arizona.edu> Does formalin fixation (or Bouins) inactivate Herpes B virus that may have been passed on from infected Macaque monkeys? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From TanyaAbbott <@t> catholichealth.net Fri Mar 28 07:48:31 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Fri Mar 28 07:48:39 2014 Subject: [Histonet] ANP.22970 Message-ID: <852F7D2C14FB464D80E182B15DB138AF3066311C@CHIEX005.CHI.catholichealth.net> Per CAP checklist ANP.22970, it lists the expected published benchmarks for ER/PR, does anyone know where to find them for Her2 or MMR? Thanks and Happy Friday!! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From kim.tournear <@t> yahoo.com Fri Mar 28 11:11:52 2014 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Fri Mar 28 11:11:59 2014 Subject: [Histonet] Sox10 Message-ID: <288352FA-8712-426B-B666-1B5791211D60@yahoo.com> Does anyone have a protocol they'd be willing to share for sox10? We use the ventana benchmark xt. Thank in advance. Sent from the iPhone of Kim Tournear ?? ? From oneilb <@t> wvuhealthcare.com Fri Mar 28 12:43:46 2014 From: oneilb <@t> wvuhealthcare.com (O'neil, Beth) Date: Fri Mar 28 12:43:56 2014 Subject: [Histonet] RE: ANP.22970 Message-ID: <3CEB8EBCF9C7A648B9694B5696462A712880DD2B@NT-EXMB2.wvuh.wvuhs.com> Per CAP checklist ANP.22970, it lists the expected published benchmarks for ER/PR, does anyone know where to find them for Her2 or MMR? Thanks and Happy Friday!! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net In response to Tanya's question, when I called CAP, they told me that if you were to view the Master Checklist, not your customized, it will list several references which I have copied for you below: REFERENCES 1) Wolff AC, Hammond ME. Schwartz IN. et al. American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. Arch Pathol Lab Med 2007;131: 18-43 2) Hammond ME. Hayes OF. Oowsett M. et al. American Society of Clinical Oncology/College of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer. Arch Pathol Lab Med 2010;134(6): 907-922 3) Fitzgibbons PL. Murphy OA. Hammond ME. et al. Recommendations for validating estrogen and progesterone receptor immunohistochemistry assays. Arch Pathol Lab Med 201 0; 134:930-935 4) Ounnwald LK. Rossing MA. Li CI. Hormone receptor status. tumor characteristics. and prognosis: a prospective cohort of breast cancer patients. Breast Cancer Research 2007;9:R6 Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor/Technical Specialist West Virginia University Hospitals oneilb@wvuhealthcare.com 304-293-7629 (office) 304-293-6014 (lab) From joewalker <@t> rrmc.org Fri Mar 28 12:49:10 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Mar 28 12:49:20 2014 Subject: [Histonet] RE: Leica Reichert Jung Cryocut 1800 In-Reply-To: <7578207839F50248A7A6CD33517295EA4F13B680@MCL-EXMB03.mfldclin.org> References: <7578207839F50248A7A6CD33517295EA4F13B680@MCL-EXMB03.mfldclin.org> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC18267424@RRMBX03.rrmc.local> I've got a 2000 model manual if that would help but not specifically what you are looking for unfortunately. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of King, Laurie J Sent: Friday, March 21, 2014 1:46 PM To: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Leica Reichert Jung Cryocut 1800 Hello all, Looking for a manual for a Leica Reichert Jung Cryocut 1800. Laurie ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From joewalker <@t> rrmc.org Fri Mar 28 12:52:00 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Mar 28 12:52:07 2014 Subject: [Histonet] Tissue Tek II Cryostat In-Reply-To: <7578207839F50248A7A6CD33517295EA4F13B680@MCL-EXMB03.mfldclin.org> References: <7578207839F50248A7A6CD33517295EA4F13B680@MCL-EXMB03.mfldclin.org> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1826747F@RRMBX03.rrmc.local> Hello everyone, Is there any value to a Tissue Tek II Cryostat? We have an old one we are willing to find a new home for as it is out of service in my institution. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From badzrosari <@t> yahoo.com Fri Mar 28 22:54:15 2014 From: badzrosari <@t> yahoo.com (Bernadette Del Rosario) Date: Fri Mar 28 22:48:56 2014 Subject: [Histonet] IHC VALIDATION In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E022989E8@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E022989E8@HHCEXCHMB03.hhcsystem.org> Message-ID: Please share to me as well.thanks.. > On Mar 27, 2014, at 5:06 PM, "Cartun, Richard" wrote: > > I keep an Excel spreadsheet on all my antibodies (and probes). I list the case number, the tissue tested, the result, "is this the expected result?", diagnosis and/or comments, and the detection system used. I am a firm believer of maintaining a "prospective" validation meaning I add positive and negative cases to the spreadsheet even after the primary validation is completed. This allows you to monitor for analytical drift, and also proves useful in identifying cases for educational purposes and for identifying control material. I will send you (and anyone else who is interested) a copy of the blank template. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org > Sent: Thursday, March 27, 2014 9:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC VALIDATION > > Histonetters > does anyone have a validation form that reflects the new principles of validation out there that would be willing to share??? > > Thanks > Histology/Cytology Supervisor > S. Kathy Baldwin, SCT (ASCP) > Memorial Hospital and Health Care Center sbaldwin@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 > > "Christ's healing mission of compassion empowers us to be for others through quality and excellence." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From santij1 <@t> bellsouth.net Sat Mar 29 16:46:24 2014 From: santij1 <@t> bellsouth.net (JERRY SANTIAGO) Date: Sat Mar 29 16:53:11 2014 Subject: [Histonet] Florida Society for Histotechnology 2014 Message-ID: <1396129584.43653.YahooMailNeo@web180906.mail.ne1.yahoo.com> Come along and sing a song And join the jamboree! May 15-18, 2014 Buena Vista Palace Hotel & Spa Lake Buena Vista, Florida From yujie.wen <@t> louisville.edu Mon Mar 31 09:35:33 2014 From: yujie.wen <@t> louisville.edu (Wen,Yujie) Date: Mon Mar 31 09:35:56 2014 Subject: [Histonet] Protocol for Periodic-Acid Schiff (PAS) Stain for human kidney biospy sample Message-ID: Our lab are looking for a protocol for PAS stain in human kidney biopsy sample. We have not done the special stain in our lab before. Could you help us out to provide an effective working SOP for the staining. Sharing the tips, thoughts about the critical steps are welcome. Many Thanks, Yujie Wen Yujie Wen, M.D., Ph.D. Research Associate Institute for Cellular Therapeutics, University of Louisville 570 South Preston Street, Baxter I Bldg. 409 Louisville, KY 40202 From Vickroy.Jim <@t> mhsil.com Mon Mar 31 10:20:24 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Mar 31 10:20:33 2014 Subject: [Histonet] Digital Camera for a H-600 Transmission electron Microscope Message-ID: Is anybody aware of companies that retrofit a digital camera for a older transmission electron microscope? They are getting rid of our darkroom and trying to decide what to do next. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From sara.stevens <@t> advocatehealth.com Mon Mar 31 14:12:45 2014 From: sara.stevens <@t> advocatehealth.com (Stevens, Sara) Date: Mon Mar 31 14:12:55 2014 Subject: [Histonet] Bone Marrow smears sent for Giemsa staining Message-ID: <2781fed161cd4e37913b8dee90e70791@CO2PR07MB522.namprd07.prod.outlook.com> Has anyone experienced poor staining of bone marrow smears that were sent from a doctor's office to a central laboratory? We have some cases of poor staining that we are thinking may be from fume contamination from the fixative bottle. Has anyone experienced anything of this nature? Thank you for your help. Sara Stevens HTL (ASCP) ACL Laboratories This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. From rlhenshall_powell <@t> yahoo.co.uk Mon Mar 31 15:40:55 2014 From: rlhenshall_powell <@t> yahoo.co.uk (Rhonda Henshall-Powell) Date: Mon Mar 31 15:41:01 2014 Subject: [Histonet] Now seeking IHC QC Manager in Northern CA IHC company Message-ID: <1396298455.38636.YahooMailNeo@web172501.mail.ir2.yahoo.com> Hello, I am currently seeking applications for an open IHC QC Manager position at a well known Northern Californian Cancer Diagnostics IHC company. The candidate will be based in the San Francisco Bay Area and will be responsible for testing intermediate, final and stability product samples to demonstrate that all products meet all standards required for cGMP operations. Ensures staff compliance with established quality control practices of testing, record collection and reporting for compliance with FDA and ISO requirements. Participates in the management of all aspects of the immunohistochemistry QC laboratory ensuring that the lab maintains the highest quality of work while meeting production schedule commitments. Please email me privately with your inquiries and resume if interested. Best Regards, Rhonda Henshall-Powell rlhenshall_powell@yahoo.co.uk From JMacDonald <@t> mtsac.edu Mon Mar 31 23:33:44 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Mar 31 23:33:50 2014 Subject: [Histonet] California Society for Histotechnology Symposium Message-ID: The 38th Annual Symposium/Convention for the California Society will be held May 2-4, 2014 at the Hilton Woodland Hills in southern California. Lots of great workshops and vendors! For more information and to download the program visit: http://californiahistology.org/events.html