From Bonnie.Whitaker <@t> osumc.edu Mon Jun 2 08:23:02 2014 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Mon Jun 2 08:23:22 2014 Subject: [Histonet] does anyone do IHC for pepsin clinically? Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670DBECAFE20@EXMBOX-VP05.OSUMC.EDU> Hi All, We've had a request from a clinician to perform IHC on a GI biopsy for pepsin. Does anyone have this up and running for clinical cases, or know of a reference lab that performs this? Thanks! Bonnie Bonnie Whitaker Anatomic Pathology Operations Director Department of Pathology Division of Anatomic Pathology N305 Doan Hall, 410 West 10th Avenue, Columbus, OH, 43210 614-293-8418 Office / 614-293-2779 Fax / 614-293-5013 Pager bonnie.whitaker@osumc.edu We are committed to improving people's lives through personalized health care. From gmarcella <@t> nj-urology.com Mon Jun 2 11:06:34 2014 From: gmarcella <@t> nj-urology.com (Gail Marcella) Date: Mon Jun 2 11:06:44 2014 Subject: [Histonet] Xylene Substitute in Leica ASP 300 tissue processor Message-ID: <53B7C169C959BB43BEF79FCB77418B64362AB97DEF@njue1> Hi - I wanted to get rid of xylene in my Leica ASP tissue processor and change to a xylene substitute such as Stat Lab's" X3", or EKI's" Safeclear". I manage a Urology Lab and have mostly small prostate and bladder biospies. Does anyone know if the times must be changed in each of the solutions in order to change over? Thanks - Gail From wesley.minto <@t> gmail.com Mon Jun 2 15:57:57 2014 From: wesley.minto <@t> gmail.com (Wesley C. Minto) Date: Mon Jun 2 15:58:03 2014 Subject: [Histonet] Is antigen retrieval open on the Leica BOND RX IHC autostainer? Message-ID: Hello Everyone, As the title would imply, can anyone speak to the ability to use third-party (i.e. lab made, BD Pharm, etc.) antigen retrieval in the Leica BOND RX automatic IHC stainer? Thanks in advance for the help. Best Regards, Wes -- Wesley C. Minto Research & Drug Discovery 530-300-3235 From Sylvia.Shockey <@t> clinicalpathologyassoc.com Mon Jun 2 16:19:38 2014 From: Sylvia.Shockey <@t> clinicalpathologyassoc.com (Sylvia Shockey) Date: Mon Jun 2 16:19:47 2014 Subject: [Histonet] Microtome Message-ID: Hello, My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished. I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker. Thanks for your time. From bcooper <@t> chla.usc.edu Mon Jun 2 17:04:45 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Mon Jun 2 17:04:53 2014 Subject: [Histonet] RE: Microtome In-Reply-To: References: Message-ID: Don't know if you can get one, but far and away, my favorite microtome of all time is the Microm HM-325 (not the newer HM325-2's). Block orientation can be accomplished one handed because of the positioning of the orientation screws (X orientation at left, and Y atop the chuck). The plate on the knife holder and base upon which rests the blade carrier assembly is black in color. Doesn't sound like much, but this helps tremendously in visually aligning the block face to the knife when you have to recut blocks. And these are the easiest microtomes in the world to adjust. I was a supervisor at a reference lab with 14 of these microtomes. I've "repaired" these when someone has overtightened levers on far too many occasions to count and it is a snap to do so. All you need is a set of allen wrenches and a screwdriver or two. Good luck! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvia Shockey Sent: Monday, June 02, 2014 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Hello, My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished. I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker. Thanks for your time. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From joelleweaver <@t> hotmail.com Mon Jun 2 18:48:04 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Jun 2 18:48:08 2014 Subject: [Histonet] RE: Microtome In-Reply-To: References: , Message-ID: Also my favorite Joelle Weaver MAOM, HTL (ASCP) QIHC > From: bcooper@chla.usc.edu > To: Sylvia.Shockey@clinicalpathologyassoc.com; histonet@lists.utsouthwestern.edu > Date: Mon, 2 Jun 2014 22:04:45 +0000 > CC: > Subject: [Histonet] RE: Microtome > > Don't know if you can get one, but far and away, my favorite microtome of all time is the Microm HM-325 (not the newer HM325-2's). Block orientation can be accomplished one handed because of the positioning of the orientation screws (X orientation at left, and Y atop the chuck). The plate on the knife holder and base upon which rests the blade carrier assembly is black in color. Doesn't sound like much, but this helps tremendously in visually aligning the block face to the knife when you have to recut blocks. And these are the easiest microtomes in the world to adjust. I was a supervisor at a reference lab with 14 of these microtomes. I've "repaired" these when someone has overtightened levers on far too many occasions to count and it is a snap to do so. All you need is a set of allen wrenches and a screwdriver or two. > > Good luck! > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvia Shockey > Sent: Monday, June 02, 2014 2:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome > > > Hello, > My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished. I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker. Thanks for your time. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Mon Jun 2 21:44:04 2014 From: suetp918 <@t> comcast.net (Sue) Date: Mon Jun 2 21:44:32 2014 Subject: [Histonet] Microtome In-Reply-To: References: Message-ID: Leica all the way 2025 Sent from my iPhone > On Jun 2, 2014, at 5:19 PM, Sylvia Shockey wrote: > > > Hello, > My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished. I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker. Thanks for your time. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Tue Jun 3 02:25:34 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Jun 3 02:26:07 2014 Subject: [Histonet] Microtome In-Reply-To: References: Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FBAFC75DE@FWDCWPMSGCMS09.hca.corpad.net> Leica 2125 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Monday, June 02, 2014 10:44 PM To: Sylvia Shockey Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Leica all the way 2025 Sent from my iPhone > On Jun 2, 2014, at 5:19 PM, Sylvia Shockey wrote: > > > Hello, > My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished. I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker. Thanks for your time. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve8438 <@t> gmail.com Tue Jun 3 04:59:12 2014 From: steve8438 <@t> gmail.com (steven Mello) Date: Tue Jun 3 04:59:26 2014 Subject: [Histonet] Microtome In-Reply-To: References: Message-ID: <0b8201cf7f12$7982ae20$6c880a60$@gmail.com> Leica is an outstanding product. Highly recommend there microtome. Steven Mello,HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Monday, June 2, 2014 10:44 PM To: Sylvia Shockey Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Leica all the way 2025 Sent from my iPhone > On Jun 2, 2014, at 5:19 PM, Sylvia Shockey wrote: > > > Hello, > My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished. I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker. Thanks for your time. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Jun 3 06:43:32 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jun 3 06:43:35 2014 Subject: [Histonet] Is antigen retrieval open on the Leica BOND RX IHC autostainer? In-Reply-To: References: Message-ID: I don't have the research toggle, but my understanding while at Leica training was that this provided a good amount of flexibility and "open-ness". Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 2 Jun 2014 13:57:57 -0700 > From: wesley.minto@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Is antigen retrieval open on the Leica BOND RX IHC autostainer? > > Hello Everyone, > > As the title would imply, can anyone speak to the ability to use > third-party (i.e. lab made, BD Pharm, etc.) antigen retrieval in the Leica > BOND RX automatic IHC stainer? Thanks in advance for the help. > > Best Regards, > > Wes > > -- > Wesley C. Minto > Research & Drug Discovery > > 530-300-3235 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amurvosh <@t> advancederm.net Tue Jun 3 09:11:05 2014 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Tue Jun 3 09:11:18 2014 Subject: [Histonet] Microtome In-Reply-To: References: Message-ID: <4AD6A4E531E8C943A730559B6B81DF07DA910B@dc.Advancederm.net> Most companies will let you test run their newest microtomes. Let them come to you and test for a week or so than pick the one you like best. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvia Shockey Sent: Monday, June 02, 2014 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Hello, My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished. I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker. Thanks for your time. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jun 3 10:08:29 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 3 10:12:03 2014 Subject: [Histonet] Microtome In-Reply-To: <4AD6A4E531E8C943A730559B6B81DF07DA910B@dc.Advancederm.net> References: <4AD6A4E531E8C943A730559B6B81DF07DA910B@dc.Advancederm.net> Message-ID: <1401808109.32583.YahooMailNeo@web120404.mail.ne1.yahoo.com> When a microtome?starts to "act up" the best solution is to give it an overhaul. If with the possibility of buying one, contact Leica or Sakura first. Both manufacture very reliable and durable instruments. Ren? J.? On Tuesday, June 3, 2014 10:12 AM, Anne Murvosh wrote: Most companies will let you test run their newest microtomes. Let them come to you and test for a week or so than pick the one you like best. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvia Shockey Sent: Monday, June 02, 2014 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome Hello, ? ? ? ? ? ? ? ? ? My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished.? I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker.? Thanks for your time. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Tue Jun 3 11:24:13 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Jun 3 11:24:26 2014 Subject: [Histonet] Microtome In-Reply-To: References: Message-ID: Leica 2125 Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Monday, June 02, 2014 7:44 PM To: Sylvia Shockey Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Leica all the way 2025 Sent from my iPhone > On Jun 2, 2014, at 5:19 PM, Sylvia Shockey wrote: > > > Hello, > My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished. I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker. Thanks for your time. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rford <@t> hcmhcares.org Tue Jun 3 11:38:09 2014 From: rford <@t> hcmhcares.org (Rhonda Ford) Date: Tue Jun 3 11:38:19 2014 Subject: [Histonet] Cytopathology (non-gynecological) Exclusion List Message-ID: Please give me advice on what your lab is doing for this. Thanks. -- Rhonda Ford (HT)ASCP, Histology Lab Henry County Hospital (765) 521-1148 From Smith_D <@t> kids.wustl.edu Tue Jun 3 12:18:06 2014 From: Smith_D <@t> kids.wustl.edu (Smith, Denise) Date: Tue Jun 3 12:18:20 2014 Subject: [Histonet] HTL Online Courses? Message-ID: <7578651EA53E304B96B06AD6D7B8992C128DAEB2@pcfmbx01.wusm-pcf.wustl.edu> Hi all! I have been doing massive research on this for past several days but no solid leads. I am wondering about HT/HTL Histology online courses... I live in St. Louis Missouri and they don't offer any histology courses around here - only out of states. I cannot relocate or do 1-2 years program out of state due to my full-time job and family reason. Do anyone know any good Histology online courses that I can take without conflicting with my job? Greatly appericated! Thanks! Denise Smith smith_d@kids.wustl.edu The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From Terra.Wineman <@t> novusint.com Tue Jun 3 12:24:45 2014 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Tue Jun 3 12:24:52 2014 Subject: [Histonet] RE: HTL Online Courses? In-Reply-To: <7578651EA53E304B96B06AD6D7B8992C128DAEB2@pcfmbx01.wusm-pcf.wustl.edu> References: <7578651EA53E304B96B06AD6D7B8992C128DAEB2@pcfmbx01.wusm-pcf.wustl.edu> Message-ID: <1EB8F245A303564EADF12AC7022FA74DA9CDCBC3@NOVUS-EX01.novusint.com> Hartford Community College in Maryland has an online course. I would also look at Indiana University. Terra Wineman, HTL (ASCP)CM Research Biologist, Nutrional Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise Sent: Tuesday, June 03, 2014 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HTL Online Courses? Hi all! I have been doing massive research on this for past several days but no solid leads. I am wondering about HT/HTL Histology online courses... I live in St. Louis Missouri and they don't offer any histology courses around here - only out of states. I cannot relocate or do 1-2 years program out of state due to my full-time job and family reason. Do anyone know any good Histology online courses that I can take without conflicting with my job? Greatly appericated! Thanks! Denise Smith smith_d@kids.wustl.edu The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Tue Jun 3 12:32:44 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Jun 3 12:34:21 2014 Subject: [Histonet] RE: HTL Online Courses? In-Reply-To: <1EB8F245A303564EADF12AC7022FA74DA9CDCBC3@NOVUS-EX01.novusint.com> References: <7578651EA53E304B96B06AD6D7B8992C128DAEB2@pcfmbx01.wusm-pcf.wustl.edu>, <1EB8F245A303564EADF12AC7022FA74DA9CDCBC3@NOVUS-EX01.novusint.com> Message-ID: <0B8979A204680A42B93A52B486088CD939386367C1@CUAEXH1.GCU-MD.local> Harford is an AA program which would not qualify you for an HTL, unless you already have a BS in Science, which in that case you have other avenues of eligibility for the exam. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wineman, Terra [Terra.Wineman@novusint.com] Sent: Tuesday, June 03, 2014 1:24 PM To: Smith, Denise; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: HTL Online Courses? Hartford Community College in Maryland has an online course. I would also look at Indiana University. Terra Wineman, HTL (ASCP)CM Research Biologist, Nutrional Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise Sent: Tuesday, June 03, 2014 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HTL Online Courses? Hi all! I have been doing massive research on this for past several days but no solid leads. I am wondering about HT/HTL Histology online courses... I live in St. Louis Missouri and they don't offer any histology courses around here - only out of states. I cannot relocate or do 1-2 years program out of state due to my full-time job and family reason. Do anyone know any good Histology online courses that I can take without conflicting with my job? Greatly appericated! Thanks! Denise Smith smith_d@kids.wustl.edu The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From rmire <@t> cvpath.org Tue Jun 3 12:34:31 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Tue Jun 3 12:34:40 2014 Subject: [Histonet] HTL Online Courses? In-Reply-To: <1EB8F245A303564EADF12AC7022FA74DA9CDCBC3@NOVUS-EX01.novusint.com> References: <7578651EA53E304B96B06AD6D7B8992C128DAEB2@pcfmbx01.wusm-pcf.wustl.edu> <1EB8F245A303564EADF12AC7022FA74DA9CDCBC3@NOVUS-EX01.novusint.com> Message-ID: <799F711F-B55E-4D55-BE53-DE8C96FF3273@cvpath.org> IUPUI has a fantastic program. Ronda Mire On Jun 3, 2014, at 1:24 PM, "Wineman, Terra" wrote: > Hartford Community College in Maryland has an online course. I would also look at Indiana University. > > Terra Wineman, HTL (ASCP)CM > Research Biologist, Nutrional Physiology > 636-926-7476 phone > terra.wineman@novusint.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise > Sent: Tuesday, June 03, 2014 12:18 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] HTL Online Courses? > > Hi all! > > I have been doing massive research on this for past several days but no solid leads. I am wondering about HT/HTL Histology online courses... I live in St. Louis Missouri and they don't offer any histology courses around here - only out of states. I cannot relocate or do 1-2 years program out of state due to my full-time job and family reason. > > Do anyone know any good Histology online courses that I can take without conflicting with my job? > > Greatly appericated! Thanks! > > Denise Smith > > smith_d@kids.wustl.edu > > > > > The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Jun 3 12:42:12 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Jun 3 12:42:21 2014 Subject: [Histonet] RE: HTL Online Courses? In-Reply-To: <1EB8F245A303564EADF12AC7022FA74DA9CDCBC3@NOVUS-EX01.novusint.com> References: <7578651EA53E304B96B06AD6D7B8992C128DAEB2@pcfmbx01.wusm-pcf.wustl.edu> <1EB8F245A303564EADF12AC7022FA74DA9CDCBC3@NOVUS-EX01.novusint.com> Message-ID: <761E2B5697F795489C8710BCC72141FF36785D02@ex07.net.ucsf.edu> Denise, Darton College, Georgia, has an online HT degree course http://www.darton.edu/programs/AlliedHealth/certificates/Hist-Cert.php Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wineman, Terra Sent: Tuesday, June 03, 2014 10:25 AM To: Smith, Denise; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: HTL Online Courses? Hartford Community College in Maryland has an online course. I would also look at Indiana University. Terra Wineman, HTL (ASCP)CM Research Biologist, Nutrional Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise Sent: Tuesday, June 03, 2014 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HTL Online Courses? Hi all! I have been doing massive research on this for past several days but no solid leads. I am wondering about HT/HTL Histology online courses... I live in St. Louis Missouri and they don't offer any histology courses around here - only out of states. I cannot relocate or do 1-2 years program out of state due to my full-time job and family reason. Do anyone know any good Histology online courses that I can take without conflicting with my job? Greatly appericated! Thanks! Denise Smith smith_d@kids.wustl.edu The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamie.erickson <@t> abbvie.com Tue Jun 3 12:43:48 2014 From: jamie.erickson <@t> abbvie.com (Erickson, Jamie E) Date: Tue Jun 3 12:43:51 2014 Subject: [Histonet] Is antigen retrieval open on the Leica BOND RX IHC Message-ID: <8B946A68A8F3534A99CC493DEFB49B1019725F7B@WM10006P.oneabbott.com> Hi Wes, We have 3 bond RX units and use them for animal tissue staining and we try all kinds of reagents (ab, chromogens, counterstains, acid washes) . As far as the HEIR and Enzyme go Leica has their own HEIR (citrate & EDTA) and an Enzyme (PTK). You can run a Dako PTK which I do at room temp or at 37C but as far as the HEIR you are limited to their Citrate Ph= 6 and EDTA Ph=9, I think I'm right on the Ph's but not sure. Anyway, we have found that all of our Dako protocols using PT modules or Pressure cooker transferred nicely to Leica and there recommended times for citrate and EDTA are pretty darn good. It is a learning curve and there are Leica ways of doing things but you can get to do almost anything you can do on the bench. We are working up a lot of double now and there are so many ways to do it. Hope that helps. Jamie JAMIE ERICKSON Scientist II HTL (ASCP)CM,MS Abbvie Bioreseach Center Pharmacology 100 Research Dr. Worcester,Ma 01605 OFFICE ? ?+1 508-688-3134 FAX ?????????+1 508-793-4895 EMAIL ?jamie.erickson@abbvie.com abbvie.com This communication may contain information that is proprietary, confidential, or exempt from disclosure. If you are not the intended recipient, please note that any other dissemination, distribution, use or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately by telephone or by return e-mail and delete it from his or her computer. ****************** From wesley.minto <@t> gmail.com Tue Jun 3 13:01:02 2014 From: wesley.minto <@t> gmail.com (Wesley C. Minto) Date: Tue Jun 3 13:01:08 2014 Subject: [Histonet] Is antigen retrieval open on the Leica BOND RX IHC In-Reply-To: <8B946A68A8F3534A99CC493DEFB49B1019725F7B@WM10006P.oneabbott.com> References: <8B946A68A8F3534A99CC493DEFB49B1019725F7B@WM10006P.oneabbott.com> Message-ID: Hey Jamie, Thanks for the feedback. That is very useful to know. I was hoping that an open container in the reagent rack might be used for AR, but it sounds like we are limited to the bulk containers. This might make the difference in choosing the Discovery Ultra over the Bond RX. Thanks again. Wes On Tue, Jun 3, 2014 at 10:43 AM, Erickson, Jamie E < jamie.erickson@abbvie.com> wrote: > Hi Wes, > We have 3 bond RX units and use them for animal tissue > staining and we try all kinds of reagents (ab, chromogens, counterstains, > acid washes) . As far as the HEIR and Enzyme go Leica has their own HEIR > (citrate & EDTA) and an Enzyme (PTK). You can run a Dako PTK which I do at > room temp or at 37C but as far as the HEIR you are limited to their Citrate > Ph= 6 and EDTA Ph=9, I think I'm right on the Ph's but not sure. > Anyway, we have found that all of our Dako protocols using PT modules or > Pressure cooker transferred nicely to Leica and there recommended times > for citrate and EDTA are pretty darn good. It is a learning curve and > there are Leica ways of doing things but you can get to do almost > anything you can do on the bench. We are working up a lot of double now and > there are so many ways to do it. > Hope that helps. > > Jamie > > JAMIE ERICKSON > Scientist II > HTL (ASCP)CM,MS > > > Abbvie Bioreseach Center > Pharmacology > 100 Research Dr. > Worcester,Ma 01605 > OFFICE +1 508-688-3134 > FAX +1 508-793-4895 > EMAIL jamie.erickson@abbvie.com > abbvie.com > > This communication may contain information that is proprietary, > confidential, or exempt from disclosure. If you are not the intended > recipient, please note that any other dissemination, distribution, use or > copying of this communication is strictly prohibited. Anyone who receives > this message in error should notify the sender immediately by telephone or > by return e-mail and delete it from his or her computer. > > ****************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Wesley C. Minto Research & Drug Discovery 530-300-3235 From Julia.Cates <@t> AHSS.ORG Tue Jun 3 13:26:26 2014 From: Julia.Cates <@t> AHSS.ORG (Cates, Julia) Date: Tue Jun 3 13:26:34 2014 Subject: [Histonet] Cytopathology (non-gynecological) Exclusion List Message-ID: <2EF522B30254BA4B9080B8AA40FDD94D65B35788@LKMVXCHMB32.ADVENTISTCORP.NET> Rhonda, We don't have cytology (Non GYN) specimens that get processed "routinely". In our procedure we say that it is "left to the discretion of the clinician" to decide if cytology is medically necessary for the patient. We also have a list of pathology specimens that are exempt from processing, this includes both histology and cytology specimens. I hope this helps. Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole?use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. Message: 12 Date: Tue, 3 Jun 2014 12:38:09 -0400 From: Rhonda Ford Subject: [Histonet] Cytopathology (non-gynecological) Exclusion List To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Please give me advice on what your lab is doing for this. Thanks. -- Rhonda Ford (HT)ASCP, Histology Lab Henry County Hospital (765) 521-1148 From tbraud <@t> holyredeemer.com Tue Jun 3 13:56:43 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Jun 3 14:08:16 2014 Subject: [Histonet] RE: Microtomes and Exclusion Lists In-Reply-To: <20140603161653.E8DCD1E8098@trendmess-svr.holyredeemer.local> References: <20140603161653.E8DCD1E8098@trendmess-svr.holyredeemer.local> Message-ID: 1. Microtome choices - my personal favorite is a Leica 2235. We have several in our lab, they are well liked by all. Whatever you decide, the vendor should enable you to have a decent trial period before making up your mind. 2. Non-Gyn Cytology Exclusion list The following is copied straight from my policy, I hope it helps: PURPOSE: To define the tissue specimens and fluid specimens that are required to be submitted to for pathologic and cytopathology reporting POLICY: Every tissue specimen removed from a patient must be sent to the Department of Pathology laboratory for examination by a Pathologist. All patient fluid specimens must be submitted to Cytopathology for examination. Any exceptions must be approved by a consensus of the medical staff. At the Medical Executive Meeting of November 6, 2012, the following policy was approved: PROCEDURE: All fluid specimens are to be forwarded to the Department of Pathology Laboratory with the following exceptions: 1. Fluids for which there is a low clinical suspicion for malignancy (i.e. synovial fluid, urines for routine urine analysis or as a result of catheterization during routine surgery, sputums for culture) 2. Fluids removed for therapeutic purposes and/or prior negative cytology findings (i.e. pleural effusion, ascites in a patient with CHF or portal hypertension) All patient tissue and material specimens removed during surgery are to be forwarded to the Department of Pathology with the following exceptions: 1. (a long list of surgical specimens, hardware, etc) I also keep a copy of the Medical Staff meeting minutes when the list was discussed and approved. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 12 Date: Tue, 3 Jun 2014 12:38:09 -0400 From: Rhonda Ford Subject: [Histonet] Cytopathology (non-gynecological) Exclusion List Please give me advice on what your lab is doing for this. Thanks. Rhonda Ford (HT)ASCP, Histology Lab Henry County Hospital (765) 521-1148 ************************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From pathdynamo <@t> gmail.com Tue Jun 3 15:20:03 2014 From: pathdynamo <@t> gmail.com (pathdynamo) Date: Tue Jun 3 15:20:09 2014 Subject: [Histonet] RE: Is antigen retrieval open on the Leica BOND RX IHC autostainer? Message-ID: My understanding is that you can use any HIER you want on the Bond RX, but they highly recommend their own reagents since they're optimized for it. We ended up buying a Biocare Decloaking Chamber for offline retrieval as we some of our antibodies were suboptimally retrieved on Bond RX. However, you should discuss your decision with Leica's tech support and they can modify the software to allow you to use antigen retrieval of your own using the Open Containers. We have asked them to modify the software for one of our ISH protocols and they did. I hope this helps. Basel On Tue, Jun 3, 2014 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Is antigen retrieval open on the Leica BOND RX IHC > autostainer? (Wesley C. Minto) > 2. Microtome (Sylvia Shockey) > 3. RE: Microtome (Cooper, Brian) > 4. RE: RE: Microtome (joelle weaver) > 5. Re: Microtome (Sue) > 6. RE: Microtome (Susan.Walzer@HCAHealthcare.com) > 7. RE: Microtome (steven Mello) > 8. RE: Is antigen retrieval open on the Leica BOND RX IHC > autostainer? (joelle weaver) > 9. RE: Microtome (Anne Murvosh) > 10. Re: Microtome (Rene J Buesa) > 11. RE: Microtome (Bea DeBrosse-Serra) > 12. Cytopathology (non-gynecological) Exclusion List (Rhonda Ford) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 2 Jun 2014 13:57:57 -0700 > From: "Wesley C. Minto" > Subject: [Histonet] Is antigen retrieval open on the Leica BOND RX IHC > autostainer? > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > CAFy8St2VyWvYrcyYvtS6WcR8dhJs16+1D29cK1u4-9Z1ypz_ug@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Hello Everyone, > > As the title would imply, can anyone speak to the ability to use > third-party (i.e. lab made, BD Pharm, etc.) antigen retrieval in the Leica > BOND RX automatic IHC stainer? Thanks in advance for the help. > > Best Regards, > > Wes > > -- > Wesley C. Minto > Research & Drug Discovery > > 530-300-3235 > > > > > ------------------------------ > > Message: 2 > Date: Mon, 2 Jun 2014 21:19:38 +0000 > From: Sylvia Shockey > Subject: [Histonet] Microtome > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > d9ff0fb32a7d47dca3f0edc1ca81d98a@BY2PR05MB661.namprd05.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > > Hello, > My name is Sylvia Shockey I work in a histology lab. > One of our microtomes is not working properly and would appreciate some > advice on purchasing a new one or maybe refurbished. I have had some > quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo > Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo > fisher has new and used on both of these models. Help me please I don't > want to buy a clunker. Thanks for your time. > > > > > > > > ------------------------------ > > Message: 3 > Date: Mon, 2 Jun 2014 22:04:45 +0000 > From: "Cooper, Brian" > Subject: [Histonet] RE: Microtome > To: "Sylvia Shockey" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > C12647AD4408834C8AB48FB0389C63E3313D7704@CHLAEXMBH01.LA.AD.CHLA.ORG> > Content-Type: text/plain; charset=us-ascii > > Don't know if you can get one, but far and away, my favorite microtome of > all time is the Microm HM-325 (not the newer HM325-2's). Block > orientation can be accomplished one handed because of the positioning of > the orientation screws (X orientation at left, and Y atop the chuck). The > plate on the knife holder and base upon which rests the blade carrier > assembly is black in color. Doesn't sound like much, but this helps > tremendously in visually aligning the block face to the knife when you have > to recut blocks. And these are the easiest microtomes in the world to > adjust. I was a supervisor at a reference lab with 14 of these microtomes. > I've "repaired" these when someone has overtightened levers on far too > many occasions to count and it is a snap to do so. All you need is a set > of allen wrenches and a screwdriver or two. > > Good luck! > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvia Shockey > Sent: Monday, June 02, 2014 2:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome > > > Hello, > My name is Sylvia Shockey I work in a histology lab. > One of our microtomes is not working properly and would appreciate some > advice on purchasing a new one or maybe refurbished. I have had some > quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo > Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo > fisher has new and used on both of these models. Help me please I don't > want to buy a clunker. Thanks for your time. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > > --------------------------------------------------------------------- > > > > > ------------------------------ > > Message: 4 > Date: Mon, 2 Jun 2014 23:48:04 +0000 > From: joelle weaver > Subject: RE: [Histonet] RE: Microtome > To: "Cooper, Brian" , Sylvia Shockey > , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Also my favorite > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: bcooper@chla.usc.edu > > To: Sylvia.Shockey@clinicalpathologyassoc.com; > histonet@lists.utsouthwestern.edu > > Date: Mon, 2 Jun 2014 22:04:45 +0000 > > CC: > > Subject: [Histonet] RE: Microtome > > > > Don't know if you can get one, but far and away, my favorite microtome > of all time is the Microm HM-325 (not the newer HM325-2's). Block > orientation can be accomplished one handed because of the positioning of > the orientation screws (X orientation at left, and Y atop the chuck). The > plate on the knife holder and base upon which rests the blade carrier > assembly is black in color. Doesn't sound like much, but this helps > tremendously in visually aligning the block face to the knife when you have > to recut blocks. And these are the easiest microtomes in the world to > adjust. I was a supervisor at a reference lab with 14 of these microtomes. > I've "repaired" these when someone has overtightened levers on far too > many occasions to count and it is a snap to do so. All you need is a set > of allen wrenches and a screwdriver or two. > > > > Good luck! > > > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvia Shockey > > Sent: Monday, June 02, 2014 2:20 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Microtome > > > > > > Hello, > > My name is Sylvia Shockey I work in a histology lab. > One of our microtomes is not working properly and would appreciate some > advice on purchasing a new one or maybe refurbished. I have had some > quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo > Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo > fisher has new and used on both of these models. Help me please I don't > want to buy a clunker. Thanks for your time. > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------------------------------------------- > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > > is for the sole use of the intended recipient(s) and may contain > confidential > > or legally privileged information. Any unauthorized review, use, > disclosure > > or distribution is prohibited. If you are not the intended recipient, > please > > contact the sender by reply e-mail and destroy all copies of this > original message. > > > > --------------------------------------------------------------------- > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Mon, 2 Jun 2014 22:44:04 -0400 > From: Sue > Subject: Re: [Histonet] Microtome > To: Sylvia Shockey > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Leica all the way 2025 > > Sent from my iPhone > > > On Jun 2, 2014, at 5:19 PM, Sylvia Shockey < > Sylvia.Shockey@clinicalpathologyassoc.com> wrote: > > > > > > Hello, > > My name is Sylvia Shockey I work in a histology lab. > One of our microtomes is not working properly and would appreciate some > advice on purchasing a new one or maybe refurbished. I have had some > quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo > Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo > fisher has new and used on both of these models. Help me please I don't > want to buy a clunker. Thanks for your time. > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Tue, 3 Jun 2014 02:25:34 -0500 > From: > Subject: RE: [Histonet] Microtome > To: , > > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > < > 4BF03F5404EBDE409AF9232DA74B9DED2FBAFC75DE@FWDCWPMSGCMS09.hca.corpad.net> > > Content-Type: text/plain; charset="us-ascii" > > Leica 2125 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > Sent: Monday, June 02, 2014 10:44 PM > To: Sylvia Shockey > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Microtome > > Leica all the way 2025 > > Sent from my iPhone > > > On Jun 2, 2014, at 5:19 PM, Sylvia Shockey < > Sylvia.Shockey@clinicalpathologyassoc.com> wrote: > > > > > > Hello, > > My name is Sylvia Shockey I work in a histology lab. > One of our microtomes is not working properly and would appreciate some > advice on purchasing a new one or maybe refurbished. I have had some > quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo > Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo > fisher has new and used on both of these models. Help me please I don't > want to buy a clunker. Thanks for your time. > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Tue, 3 Jun 2014 05:59:12 -0400 > From: "steven Mello" > Subject: RE: [Histonet] Microtome > To: "'Sue'" , "'Sylvia Shockey'" > > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <0b8201cf7f12$7982ae20$6c880a60$@gmail.com> > Content-Type: text/plain; charset="us-ascii" > > Leica is an outstanding product. Highly recommend there microtome. > > Steven Mello,HT(ASCP) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > Sent: Monday, June 2, 2014 10:44 PM > To: Sylvia Shockey > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Microtome > > Leica all the way 2025 > > Sent from my iPhone > > > On Jun 2, 2014, at 5:19 PM, Sylvia Shockey > wrote: > > > > > > Hello, > > My name is Sylvia Shockey I work in a histology lab. > One > of our microtomes is not working properly and would appreciate some advice > on purchasing a new one or maybe refurbished. I have had some quotes on a > Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon > Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has > new and used on both of these models. Help me please I don't want to buy a > clunker. Thanks for your time. > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 8 > Date: Tue, 3 Jun 2014 11:43:32 +0000 > From: joelle weaver > Subject: RE: [Histonet] Is antigen retrieval open on the Leica BOND RX > IHC autostainer? > To: "Wesley C. Minto" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > I don't have the research toggle, but my understanding while at Leica > training was that this provided a good amount of flexibility and > "open-ness". > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > Date: Mon, 2 Jun 2014 13:57:57 -0700 > > From: wesley.minto@gmail.com > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Is antigen retrieval open on the Leica BOND RX IHC > autostainer? > > > > Hello Everyone, > > > > As the title would imply, can anyone speak to the ability to use > > third-party (i.e. lab made, BD Pharm, etc.) antigen retrieval in the > Leica > > BOND RX automatic IHC stainer? Thanks in advance for the help. > > > > Best Regards, > > > > Wes > > > > -- > > Wesley C. Minto > > Research & Drug Discovery > > > > 530-300-3235 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 9 > Date: Tue, 3 Jun 2014 07:11:05 -0700 > From: "Anne Murvosh" > Subject: RE: [Histonet] Microtome > To: "Sylvia Shockey" , > > Message-ID: > <4AD6A4E531E8C943A730559B6B81DF07DA910B@dc.Advancederm.net> > Content-Type: text/plain; charset="us-ascii" > > Most companies will let you test run their newest microtomes. Let them > come to you and test for a week or so than pick the one you like best. > Anne > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvia > Shockey > Sent: Monday, June 02, 2014 2:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome > > > Hello, > My name is Sylvia Shockey I work in a histology lab. > One of our microtomes is not working properly and would appreciate some > advice on purchasing a new one or maybe refurbished. I have had some > quotes on a Tanner scientific manual rotary microtome, titan 5000 . > Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with > Thermo fisher has new and used on both of these models. Help me please I > don't want to buy a clunker. Thanks for your time. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 10 > Date: Tue, 3 Jun 2014 08:08:29 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Microtome > To: Anne Murvosh , Sylvia Shockey > , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1401808109.32583.YahooMailNeo@web120404.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > When a microtome starts to "act up" the best solution is to give it an > overhaul. If with the possibility of buying one, contact Leica or Sakura > first. Both manufacture very reliable and durable instruments. > Ren? J. > > > On Tuesday, June 3, 2014 10:12 AM, Anne Murvosh > wrote: > > > > Most companies will let you test run their newest microtomes. Let them > come to you and test for a week or so than pick the one you like best. > Anne > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sylvia > Shockey > Sent: Monday, June 02, 2014 2:20 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtome > > > Hello, > My name is Sylvia Shockey I work in a histology lab. > One of our microtomes is not working properly and would appreciate some > advice on purchasing a new one or maybe refurbished. I have had some > quotes on a Tanner scientific manual rotary microtome, titan 5000 . > Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with > Thermo fisher has new and used on both of these models. Help me please I > don't want to buy a clunker. Thanks for your time. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 11 > Date: Tue, 3 Jun 2014 16:24:13 +0000 > From: Bea DeBrosse-Serra > Subject: RE: [Histonet] Microtome > To: Sue , Sylvia Shockey > > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Leica 2125 > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > Sent: Monday, June 02, 2014 7:44 PM > To: Sylvia Shockey > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Microtome > > Leica all the way 2025 > > Sent from my iPhone > > > On Jun 2, 2014, at 5:19 PM, Sylvia Shockey < > Sylvia.Shockey@clinicalpathologyassoc.com> wrote: > > > > > > Hello, > > My name is Sylvia Shockey I work in a histology lab. > One of our microtomes is not working properly and would appreciate some > advice on purchasing a new one or maybe refurbished. I have had some > quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo > Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo > fisher has new and used on both of these models. Help me please I don't > want to buy a clunker. Thanks for your time. > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Tue, 3 Jun 2014 12:38:09 -0400 > From: Rhonda Ford > Subject: [Histonet] Cytopathology (non-gynecological) Exclusion List > To: histonet@lists.utsouthwestern.edu > Message-ID: > F5XK4C4i5YxHiA@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Please give me advice on what your lab is doing for this. Thanks. > > -- > Rhonda Ford (HT)ASCP, Histology Lab > Henry County Hospital > (765) 521-1148 > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 127, Issue 2 > **************************************** > From hlukey <@t> msn.com Tue Jun 3 15:31:13 2014 From: hlukey <@t> msn.com (Hugh Luk) Date: Tue Jun 3 15:31:17 2014 Subject: [Histonet] RE: Histonet Digest, Vol 127, Issue 2 In-Reply-To: References: Message-ID: Sylvia, I per diem at a lab that has four Microm HM355 S microtomes (previous generation), and they work well; easy to get used to and dependable when serviced properly. Your HM 325 appears to have a similar mechanism that served their needs for years. The Thermo Finesse is also a decent machine. Another lab had a ME (motorized) version. Also easy to get used to and dependable. My only concern was a short disposable knife holder without side guards, but I see that is not offered anymore. Tanner has been around for awhile. Have not experience their handiwork. Ask for references if they cannot let you try one first. Used? I can't really recommend them as I got super-burned when I let an administrator buy used equipment. Perhaps you can get someone dependable to vouch for the instrument plus a return policy.(?) Lastly, my lab has several Sakura Accu-cut SRM 200s and Leica RM2255s. Small labs with low volume and little $ will appreciate Sakura. Leica models are most histotechs gold-standard but are pricier (deservedly so). Service? Also find out who can service your unit. A local bio-technician is preferred. My $0.02. Hope something here is helpful. Hugh Hawaii > ------------------------------ > Date: Mon, 2 Jun 2014 21:19:38 +0000 > From: Sylvia Shockey > Subject: [Histonet] Microtome > To: "histonet@lists.utsouthwestern.edu" > > Hello, > My name is Sylvia Shockey I work in a histology lab. One of our microtomes is not working properly and would appreciate some advice on purchasing a new one or maybe refurbished. I have had some quotes on a Tanner scientific manual rotary microtome, titan 5000 . Thermo Shandon Finesse and a Thermo Shandon Microm HM325. Mr. Grimm with Thermo fisher has new and used on both of these models. Help me please I don't want to buy a clunker. Thanks for your time. _______________________________________________ > **************************************** From optimusprimehistotech <@t> hotmail.com Tue Jun 3 15:34:51 2014 From: optimusprimehistotech <@t> hotmail.com (Alpha Histotech) Date: Tue Jun 3 15:34:56 2014 Subject: [Histonet] Should I leave histology world Message-ID: Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) From flnails <@t> texaschildrens.org Tue Jun 3 16:00:02 2014 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Jun 3 16:00:07 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: References: Message-ID: <327E034F1892504289B7A17EC71DF9F30B5FDF@TCFMSG03.ad.texaschildrenshospital.org> I always tell my students if you can cut, you can get a job. It appears that you school did not properly prepare you for the demands of an average histology job. You need to take every opportunity to work on your craft and the major focus of histology is cutting. With 6 to 7 years of experience you are expected to know the basics and cutting is basic. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alpha Histotech Sent: Tuesday, June 03, 2014 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Should I leave histology world Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From nmhisto <@t> comcast.net Tue Jun 3 16:28:50 2014 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Tue Jun 3 16:29:17 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: <327E034F1892504289B7A17EC71DF9F30B5FDF@TCFMSG03.ad.texaschildrenshospital.org> References: <327E034F1892504289B7A17EC71DF9F30B5FDF@TCFMSG03.ad.texaschildrenshospital.org> Message-ID: <599903481.2425987.1401830930956.JavaMail.root@comcast.net> Felton, I disagree!? The training this tech underwent must obviously have?covered basic histology but you cannot guarantee?that a trained student will find a laboratory that will give him/her the opportunity to develop speed while not sacrificing quality. Having the training does not warrant that the new tech will be able to cut a certain number of blocks per hour to satisfy the demands of an employer's?spreadsheet mentality.? A good laboratory manager will make it possible for that "newbie" to have the time at the microtome (or the embedding station, etc.) to develop the speed that comes with an experienced eye.? Providing that newly-minted tech the time necessary just makes sense to the employer and the tech.??Realizing full well (after 40+ years as an HT) that this is not a perfect world and that other factors weigh heavily, encouragement pays off in quality, quantity and loyalty.? Perhaps what Alpha Histotech needs to do (if the lab manager is an open-minded, logical individual) is to discuss this issue with that manager and allow the manager to understand that this new tech has the enthusiasm but needs "time in grade".? This is too critical an issue to begin to lose techs (as few as there are coming into the field) by an employer's requirement to produce quantity without the absolute necessity of quality. Regards to all! From nmhisto <@t> comcast.net Tue Jun 3 16:37:10 2014 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Tue Jun 3 16:37:28 2014 Subject: [Histonet] Microtome In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FBAFC75DE@FWDCWPMSGCMS09.hca.corpad.net> References: <4BF03F5404EBDE409AF9232DA74B9DED2FBAFC75DE@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: <1244188434.2430425.1401831430875.JavaMail.root@comcast.net> If someone promised me a brand new FREE microtome and it wasn't a LEICA, I'd tell them, "no, thanks!".? I am not inclined to endorse any other brand and I cannot imagine changing my mind about that. From wdesalvo.cac <@t> outlook.com Tue Jun 3 16:07:54 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Jun 3 16:43:02 2014 Subject: =?utf-8?Q?Re:_[Histonet]_Should_I_leave_histology_world?= Message-ID: Do not give up and try not to blame a process or someone else for your lack of developed skill at microtomy, but dedicate yourself to personal improvement. You have 6+ years invested in a career, and if your reasons for embarking on that career remain, then bear down and find a way to improve your microtomy technique. Work w/ a mentor, ask for help, demand helpful criticism, cut extra blocks after your scheduled work period and find a way to improve. If consistent and quality microtomy was as easy as it sounds, we would not have the shortage of technicians that exist. I look at your problem from another perspective, lack of training and competency standards. From your description, I do not think this is about quantity over quality, but one of functionality and commitment to excellence. I often hear from newly graduated students, I teach in a Histotechnology program, that they consider themselves trained and ready Histotechnologists and often expect to be highly paid without always being highly skilled. Unfortunately, new graduates are only beginners, or apprentices if you will, and must, as Felton states, work on their craftsmanship. Histotechnology has two sides, educational and functional, and the individual technicians must take responsibility for the functional side. The lab/company your work at to develop your skill is responsible for training and support. It is counter productive to resist metrics, they are hear to stay and they can be very useful. When metrics are properly used, quality and quantity are married together to develop the acceptable productivity level. Always remember that the ultimate judge of the slide quality is the person creating the slide. A pathologist may have a wide scale of acceptance, but only through a skilled technician can patient care be directly affected and the quality improved. The best path to process improvement in the Histology lab is through a trained, competent and skilled technologist/technician. Graduating from a NAACLS program is a start not the finish. Sent from Windows Mail From: Nails, Felton Sent: ?Tuesday?, ?June? ?3?, ?2014 ?2?:?00? ?PM To: Alpha Histotech, histonet I always tell my students if you can cut, you can get a job. It appears that you school did not properly prepare you for the demands of an average histology job. You need to take every opportunity to work on your craft and the major focus of histology is cutting. With 6 to 7 years of experience you are expected to know the basics and cutting is basic. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alpha Histotech Sent: Tuesday, June 03, 2014 3:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Should I leave histology world Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dwaugh <@t> kent.edu Tue Jun 3 16:51:13 2014 From: dwaugh <@t> kent.edu (David Waugh) Date: Tue Jun 3 16:51:19 2014 Subject: [Histonet] Practice tissue? Message-ID: <03217A44-2EE4-418A-BF1F-220BB4D77D1B@kent.edu> Hello histonet, I was a member a number of years ago, but had gotten out of doing much histology (I was never a pro) for awhile?. but I seem to be back at it. I was wondering if people might have some ideas on a good ?practice tissue?, one that I could embed, section, and stain (H&E) to both practice my sectioning, and check my staining protocol, etc. I really need to be sectioning some decalcified teeth, and have had some luck, but I would really like to do some more practice on an ?easy to section tissue? (other than just a blank block of paraffin) that I can also test my staining on. I have accesses to a tissue processor. Is there anything I can just pick up at the grocery store? Thanks, David David A. Waugh, Ph.D. Research Assistant Department of Anatomy and Neurobiology NEOMED 4209 State Route 44 Rootstown, OH 44272 From liz <@t> premierlab.com Tue Jun 3 17:27:17 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jun 3 17:27:23 2014 Subject: [Histonet] Practice tissue? In-Reply-To: <03217A44-2EE4-418A-BF1F-220BB4D77D1B@kent.edu> References: <03217A44-2EE4-418A-BF1F-220BB4D77D1B@kent.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05C96@SBS2K8.premierlab.local> A Slim Jim would work or any meat or lunch meat purchased at the grocery store. You will need to start by fixing the samples appropriately and then process, embed and section. If you work in a research center that does animals studies then I would see if you could not get some animal tissue even a mouse or rat would work to collect the teeth, fix properly and then decal and process, etc. Fresh tissue is always better than other types of samples. If you are close to a University that has a vivarium or veterinary school give them a call to see if you can get any fresh tissue samples from them over the store bought samples. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Waugh Sent: Tuesday, June 03, 2014 3:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Practice tissue? Hello histonet, I was a member a number of years ago, but had gotten out of doing much histology (I was never a pro) for awhile.... but I seem to be back at it. I was wondering if people might have some ideas on a good "practice tissue", one that I could embed, section, and stain (H&E) to both practice my sectioning, and check my staining protocol, etc. I really need to be sectioning some decalcified teeth, and have had some luck, but I would really like to do some more practice on an "easy to section tissue" (other than just a blank block of paraffin) that I can also test my staining on. I have accesses to a tissue processor. Is there anything I can just pick up at the grocery store? Thanks, David David A. Waugh, Ph.D. Research Assistant Department of Anatomy and Neurobiology NEOMED 4209 State Route 44 Rootstown, OH 44272 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyer3322 <@t> aol.com Tue Jun 3 17:46:50 2014 From: kdwyer3322 <@t> aol.com (Kathy Dwyer) Date: Tue Jun 3 17:46:57 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: References: Message-ID: <12971966-9E54-46F0-9D2A-8C7835D3CF75@aol.com> Bill very well put and I agree with you. Sent from my iPhone > On Jun 3, 2014, at 4:07 PM, WILLIAM DESALVO wrote: > > Do not give up and try not to blame a process or someone else for your lack of developed skill at microtomy, but dedicate yourself to personal improvement. You have 6+ years invested in a career, and if your reasons for embarking on that career remain, then bear down and find a way to improve your microtomy technique. Work w/ a mentor, ask for help, demand helpful criticism, cut extra blocks after your scheduled work period and find a way to improve. If consistent and quality microtomy was as easy as it sounds, we would not have the shortage of technicians that exist. > > > I look at your problem from another perspective, lack of training and competency standards. From your description, I do not think this is about quantity over quality, but one of functionality and commitment to excellence. I often hear from newly graduated students, I teach in a Histotechnology program, that they consider themselves trained and ready Histotechnologists and often expect to be highly paid without always being highly skilled. Unfortunately, new graduates are only beginners, or apprentices if you will, and must, as Felton states, work on their craftsmanship. Histotechnology has two sides, educational and functional, and the individual technicians must take responsibility for the functional side. The lab/company your work at to develop your skill is responsible for training and support. > > > It is counter productive to resist metrics, they are hear to stay and they can be very useful. When metrics are properly used, quality and quantity are married together to develop the acceptable productivity level. Always remember that the ultimate judge of the slide quality is the person creating the slide. A pathologist may have a wide scale of acceptance, but only through a skilled technician can patient care be directly affected and the quality improved. The best path to process improvement in the Histology lab is through a trained, competent and skilled technologist/technician. Graduating from a NAACLS program is a start not the finish. > > > > > > > Sent from Windows Mail > > > > > > From: Nails, Felton > Sent: ?Tuesday?, ?June? ?3?, ?2014 ?2?:?00? ?PM > To: Alpha Histotech, histonet > > > > > > I always tell my students if you can cut, you can get a job. It appears that you school did not properly prepare you for the demands of an average histology job. > You need to take every opportunity to work on your craft and the major focus of histology is cutting. With 6 to 7 years of experience you are expected to know the basics and cutting is basic. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alpha Histotech > Sent: Tuesday, June 03, 2014 3:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Should I leave histology world > > Hi everyone, > > I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. > > I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. > > Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. > > Thank you > Alpha Histotech (ASCP HT) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > CONFIDENTIALITY NOTICE: > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination, or > copying of this e-mail and its attachments, if any, or > the information contained herein is prohibited. If you > have received this e-mail in error, please immediately > notify the sender by return e-mail and delete this e-mail > from your computer system. Thank you. > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Jun 3 17:51:31 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Jun 3 17:51:45 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF36785FDE@ex07.net.ucsf.edu> Alpha, it is clear to me, after 30+ years in the field, that some are born with the ability to cut fast AND do well at it. The rest of us just have to work harder at developing that skill. But it does take bench time to do it. A recent cache is that it takes 10,000 hours to become an absolute expert at something - that's about 5 years full time work. And that's just one skill. It sounds like you need some good teachers (ie, those who like to teach and like having their students do well). That would be the highest priority if you want to stay in the field as a bench tech. If the factory job isn't working out why not look for a smaller lab in which you can get more extensive experience? I really value the fact that spent my first 11 years in a 4- person lab in which we did everything from grossing to histo to immunos to EM. It may pay less initially but may add more value to your lifetime career. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alpha Histotech Sent: Tuesday, June 03, 2014 1:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Should I leave histology world Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Tue Jun 3 18:17:02 2014 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Jun 3 18:17:06 2014 Subject: [Histonet] Practice tissue? In-Reply-To: <03217A44-2EE4-418A-BF1F-220BB4D77D1B@kent.edu> References: <03217A44-2EE4-418A-BF1F-220BB4D77D1B@kent.edu> Message-ID: <538E576E.8050300@shaw.ca> Go to a butcher and ask what fresh offal they have. That should be much the same as autopsy tissue. In addition, heart, liver, kidney and steak from a supermarket should be OK. If you can get some, a fresh placenta is good for H&E staining with a good mixture of elements, human or animal makes no difference. Check with a local veterinarian or animal pound. Sad to say that many unwanted animals are euthanized and their tissue might be available. If you are close to a slaughterhouse, I always found them to be very accommodating for tissue requests from pigs and so on. Rabbit breeders will sell a live rabbit for a reasonable amount, if you can bring yourself to kill it. They may even kill it for you if they farm rabbits for meat. Bryan Llewellyn David Waugh wrote: > Hello histonet, I was a member a number of years ago, but had gotten out of doing much histology (I was never a pro) for awhile?. but I seem to be back at it. > I was wondering if people might have some ideas on a good ?practice tissue?, one that I could embed, section, and stain (H&E) to both practice my sectioning, and check my staining protocol, etc. I really need to be sectioning some decalcified teeth, and have had some luck, but I would really like to do some more practice on an ?easy to section tissue? (other than just a blank block of paraffin) that I can also test my staining on. I have accesses to a tissue processor. Is there anything I can just pick up at the grocery store? Thanks, David > David A. Waugh, Ph.D. > Research Assistant > Department of Anatomy and Neurobiology > NEOMED > 4209 State Route 44 > Rootstown, OH 44272 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Tue Jun 3 19:55:09 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Jun 3 19:55:14 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: <761E2B5697F795489C8710BCC72141FF36785FDE@ex07.net.ucsf.edu> References: , <761E2B5697F795489C8710BCC72141FF36785FDE@ex07.net.ucsf.edu> Message-ID: It would be a shame to get discouraged now after all the time you have already put into histology. If you still want to work in histology, I might suggest you try to have a conversation with a manager, supervisor or lead tech and see if they are willing to support you. Tell them you want to spend more time cuting to be able to section with high quality at the rate that works for their productivity standards. If you present it as a win-win proposition, see what resources, people and time they are willing to "chip in" to help get where they would like you to be. Make some metric or rate to achieve in microtomy your goal for the year, and put it into writing ( good for all goals:). Or if that is too uncomfortable , approach someone individually whose microtomy skills you admire , and see if they are willing to provide some tips and guidance off work time. I also went through a NAACLS program. Still at my first "real" histology job , the realization that this was the actual training became apparent very quickly. I had moments of exhaustion and feeling overwhelmed, but I now feel I was also fortunate to work initially at a pretty high volume place. It was a great "breaking in" for embedding and microtomy. Luckily there were also some experienced techs there who saw how much I wanted to learn, and were willing to help me get better. The "constructive" criticism stung sometimes, but they did me a huge service. But believe me, not everyone was helpful or supportive along the way. Try to ignore those kind of people as much as possible. And I still get criticized sometimes, make mistakes, and I still have more to learn. But here are a couple of other options for you to consider before you decide to leave, and what I did to get more experience when in your situation more quickly; Take on a second histology job that targets specific skills, tissue, or techniques you want more experience in. Believe me I have been criticized and misunderstood for this choice s well many times, but personally I do not regret any of those experiences now. I also feel that small labs are nice to build well rounded skills since you are usually more of a "jack of all trades" and have less tendency to do one task over your whole shift from day to day. Sometimes you just have to identify the environment that is the right fit for you. Best of luck to you- and let us know how things turn out! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: optimusprimehistotech@hotmail.com; histonet@lists.utsouthwestern.edu > Date: Tue, 3 Jun 2014 22:51:31 +0000 > Subject: RE: [Histonet] Should I leave histology world > CC: > > Alpha, it is clear to me, after 30+ years in the field, that some are born with the ability to cut fast AND do well at it. The rest of us just have to work harder at developing that skill. But it does take bench time to do it. A recent cache is that it takes 10,000 hours to become an absolute expert at something - that's about 5 years full time work. And that's just one skill. > > It sounds like you need some good teachers (ie, those who like to teach and like having their students do well). That would be the highest priority if you want to stay in the field as a bench tech. > > If the factory job isn't working out why not look for a smaller lab in which you can get more extensive experience? I really value the fact that spent my first 11 years in a 4- person lab in which we did everything from grossing to histo to immunos to EM. It may pay less initially but may add more value to your lifetime career. > > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alpha Histotech > Sent: Tuesday, June 03, 2014 1:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Should I leave histology world > > Hi everyone, > > I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. > > I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. > > Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. > > Thank you > Alpha Histotech (ASCP HT) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Tue Jun 3 20:32:11 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Jun 3 20:32:30 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF36785FDE@ex07.net.ucsf.edu> Message-ID: <1057490404.2862137.1401845531508.JavaMail.root@comcast.net> Alpha Histotech- I'll put in?my few words even though I'm not active anymore and possibly from different perspective.? But also?using a few assumptions and if my assumptions are wrong then the rest of what I say is probably meaningless.? Not ID?ng your e-mail address but if you've worked 3 jobs nightshift including a large reference lab, do you live near a big city?? And if so is it a city close to a college or university. Research histology should not be overlooked.? You will find many molecular or other such non-histo labs that actually do some or even a lot of histology by non-histology personnel or lab workers.? Sometimes it is OK, sometimes even great.? Sometimes, and I witnessed it, it is at an embarrassing histo level.? I can walk up or down university hallways and see a "genetics lab" or some other "molecular lab" and see a microtome or cryostat in there.? Sometimes those PI's will send histo work to a core lab.? Sometimes they don't want to pay per block so do it (and staining and IHC and FISH) themselves.? Someone with even minimal wide-ranging histo experience might be welcomed. No timed block cutting counts.? Learn some immunology, genetics, molecular techniques, comparative medicine, physiology, etc, etc along the way.? Many places even pay for college level courses while employed there. Just a thought if you are near that kind of area. Ray in Seattle ----- Original Message ----- From: "joelle weaver" To: "Timothy Morken" , "Alpha Histotech" , histonet@lists.utsouthwestern.edu Sent: Tuesday, June 3, 2014 5:55:09 PM Subject: RE: [Histonet] Should I leave histology world It would be a shame to get discouraged now after all the time you have already put into histology. If you still want to work in histology, I might suggest you try to have a conversation with a manager, supervisor or lead tech and see if they are willing to support you. Tell them you want to spend more time cuting to be able to section with high quality at the rate that works for their productivity standards. ?If you present it as a win-win proposition, see what resources, people and time they are willing to "chip in" ?to help get where they would like you to be. Make some metric or rate to achieve in microtomy your goal for the year, and put it into writing ( good for all goals:). Or if that is too uncomfortable , approach someone individually whose microtomy skills you admire , and see if they are willing to provide some tips and guidance off work time. ? I also went through a NAACLS program. ?Still at my first "real" histology job , the realization that this was the actual training became apparent very quickly. ?I had moments of exhaustion and feeling overwhelmed, but I now feel I was also fortunate to work initially at a pretty high volume place. It was a great "breaking in" for embedding and microtomy. ? Luckily there were also some experienced techs there who saw how much I wanted to learn, ?and were willing to help me get better. The "constructive" criticism stung sometimes, but they did me a huge service. But believe me, ?not everyone was helpful or supportive along the way. Try to ignore those kind of people as much as possible. And I still get criticized sometimes, make mistakes, and I still have more to learn. ? But here are a couple of other options for you to consider before you decide to leave, and what ?I did to get more experience ?when in your situation more quickly; ? Take on a second histology job that targets specific skills, tissue, or techniques you want more experience in. Believe me I have been criticized and misunderstood for this choice s well many times, but personally I do not regret any of those experiences now. ? I also feel that small labs are nice to build well rounded skills since you are usually more of a "jack of all trades" and have less tendency to do one task over your whole shift from day to day. Sometimes you just have to identify the environment that is the right fit for you. ? Best of luck to you- and let us know how things turn out! Joelle Weaver MAOM, HTL (ASCP) QIHC ? > From: Timothy.Morken@ucsfmedctr.org > To: optimusprimehistotech@hotmail.com; histonet@lists.utsouthwestern.edu > Date: Tue, 3 Jun 2014 22:51:31 +0000 > Subject: RE: [Histonet] Should I leave histology world > CC: > > Alpha, it is clear to me, after 30+ years in the field, that some are born with the ability to cut fast AND do well at it. The rest of us just have to work harder at developing that skill. But it does take bench time to do it. A recent cache is that it takes 10,000 hours to become an absolute expert at something - that's about 5 years full time work. And that's just one skill. > > It sounds like you need some good teachers (ie, those who like to teach and like having their students do well). That would be the highest priority if you want to stay in the field as a bench tech. > > If the factory job isn't working out why not look for a smaller lab in which you can get more extensive experience? I really value the fact that spent my first 11 years in a 4- person lab in which we did everything from grossing to histo to immunos to EM. It may pay less initially but may add more value to your lifetime career. > > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alpha Histotech > Sent: Tuesday, June 03, 2014 1:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Should I leave histology world > > Hi everyone, > > I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. > > I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. > > Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! ?So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. ?What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. ?I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. > > Thank you > Alpha Histotech (ASCP HT) > > > ????????????????? ???????? ? ???????????????? ?_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ????????????????? ???????? ? ???????????????? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmire <@t> cvpath.org Wed Jun 4 06:00:27 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Wed Jun 4 06:00:37 2014 Subject: [Histonet] Microtome In-Reply-To: <1244188434.2430425.1401831430875.JavaMail.root@comcast.net> References: <4BF03F5404EBDE409AF9232DA74B9DED2FBAFC75DE@FWDCWPMSGCMS09.hca.corpad.net> <1244188434.2430425.1401831430875.JavaMail.root@comcast.net> Message-ID: <9C750036-974B-4B98-9FB8-E5CDB0A37EA4@cvpath.org> Agree, Leica is the best hands down. On Jun 3, 2014, at 5:37 PM, nmhisto@comcast.net wrote: > If someone promised me a brand new FREE microtome and it wasn't a LEICA, I'd tell them, "no, thanks!". I am not inclined to endorse any other brand and I cannot imagine changing my mind about that. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Jun 4 06:45:55 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jun 4 06:46:01 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: <1057490404.2862137.1401845531508.JavaMail.root@comcast.net> References: <761E2B5697F795489C8710BCC72141FF36785FDE@ex07.net.ucsf.edu> , <1057490404.2862137.1401845531508.JavaMail.root@comcast.net> Message-ID: Yes thanks for the perspective. I have a bias towards my own experience, and this seems to be good advice. I work in a molecular based lab now and they are very unaware of what it typically is like in a clinical histopathology lab. Good to point other environments are out there that are clinical, and also that research in general can be very different than clinical settings. Some people are just more suited to certain environments over the other. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 4 Jun 2014 01:32:11 +0000 From: koellingr@comcast.net To: joelleweaver@hotmail.com CC: timothy.morken@ucsfmedctr.org; optimusprimehistotech@hotmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Should I leave histology world Alpha Histotech- I'll put in my few words even though I'm not active anymore and possibly from different perspective. But also using a few assumptions and if my assumptions are wrong then the rest of what I say is probably meaningless. Not ID?ng your e-mail address but if you've worked 3 jobs nightshift including a large reference lab, do you live near a big city? And if so is it a city close to a college or university. Research histology should not be overlooked. You will find many molecular or other such non-histo labs that actually do some or even a lot of histology by non-histology personnel or lab workers. Sometimes it is OK, sometimes even great. Sometimes, and I witnessed it, it is at an embarrassing histo level. I can walk up or down university hallways and see a "genetics lab" or some other "molecular lab" and see a microtome or cryostat in there. Sometimes those PI's will send histo work to a core lab. Sometimes they don't want to pay per block so do it (and staining and IHC and FISH) themselves. Someone with even minimal wide-ranging histo experience might be welcomed. No timed block cutting counts. Learn some immunology, genetics, molecular techniques, comparative medicine, physiology, etc, etc along the way. Many places even pay for college level courses while employed there. Just a thought if you are near that kind of area. Ray in Seattle From: "joelle weaver" To: "Timothy Morken" , "Alpha Histotech" , histonet@lists.utsouthwestern.edu Sent: Tuesday, June 3, 2014 5:55:09 PM Subject: RE: [Histonet] Should I leave histology world It would be a shame to get discouraged now after all the time you have already put into histology. If you still want to work in histology, I might suggest you try to have a conversation with a manager, supervisor or lead tech and see if they are willing to support you. Tell them you want to spend more time cuting to be able to section with high quality at the rate that works for their productivity standards. If you present it as a win-win proposition, see what resources, people and time they are willing to "chip in" to help get where they would like you to be. Make some metric or rate to achieve in microtomy your goal for the year, and put it into writing ( good for all goals:). Or if that is too uncomfortable , approach someone individually whose microtomy skills you admire , and see if they are willing to provide some tips and guidance off work time. I also went through a NAACLS program. Still at my first "real" histology job , the realization that this was the actual training became apparent very quickly. I had moments of exhaustion and feeling overwhelmed, but I now feel I was also fortunate to work initially at a pretty high volume place. It was a great "breaking in" for embedding and microtomy. Luckily there were also some experienced techs there who saw how much I wanted to learn, and were willing to help me get better. The "constructive" criticism stung sometimes, but they did me a huge service. But believe me, not everyone was helpful or supportive along the way. Try to ignore those kind of people as much as possible. And I still get criticized sometimes, make mistakes, and I still have more to learn. But here are a couple of other options for you to consider before you decide to leave, and what I did to get more experience when in your situation more quickly; Take on a second histology job that targets specific skills, tissue, or techniques you want more experience in. Believe me I have been criticized and misunderstood for this choice s well many times, but personally I do not regret any of those experiences now. I also feel that small labs are nice to build well rounded skills since you are usually more of a "jack of all trades" and have less tendency to do one task over your whole shift from day to day. Sometimes you just have to identify the environment that is the right fit for you. Best of luck to you- and let us know how things turn out! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: optimusprimehistotech@hotmail.com; histonet@lists.utsouthwestern.edu > Date: Tue, 3 Jun 2014 22:51:31 +0000 > Subject: RE: [Histonet] Should I leave histology world > CC: > > Alpha, it is clear to me, after 30+ years in the field, that some are born with the ability to cut fast AND do well at it. The rest of us just have to work harder at developing that skill. But it does take bench time to do it. A recent cache is that it takes 10,000 hours to become an absolute expert at something - that's about 5 years full time work. And that's just one skill. > > It sounds like you need some good teachers (ie, those who like to teach and like having their students do well). That would be the highest priority if you want to stay in the field as a bench tech. > > If the factory job isn't working out why not look for a smaller lab in which you can get more extensive experience? I really value the fact that spent my first 11 years in a 4- person lab in which we did everything from grossing to histo to immunos to EM. It may pay less initially but may add more value to your lifetime career. > > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alpha Histotech > Sent: Tuesday, June 03, 2014 1:35 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Should I leave histology world > > Hi everyone, > > I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. > > I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. > > Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. > > Thank you > Alpha Histotech (ASCP HT) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Albert.Santiago <@t> uphs.upenn.edu Wed Jun 4 07:41:44 2014 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Wed Jun 4 07:41:55 2014 Subject: [Histonet] fluorescent scope and slide scanner Message-ID: Hello my colleagues in histoland, We are in the market for a Slide Scanner and a Fluorescent Microscope, if anyone has any information on any of these products please share with me. Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago@uphs.upenn.edu The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From James.E.Nutter <@t> questdiagnostics.com Wed Jun 4 08:19:40 2014 From: James.E.Nutter <@t> questdiagnostics.com (Nutter, James E) Date: Wed Jun 4 08:20:06 2014 Subject: [Histonet] Practice tissue? Message-ID: Go to the local pet store and get a feeder rat (can buy frozen ones). Gross it and process. Great for practice tissues, can get the full range from brain to kidney. Good luck! James E. Nutter Jr. BS, HT & QIHC(ASCP) Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.| Chantilly Va. USA | phone 703.802.6900 x65782| fax 703.802.7191| | James.E.Nutter@QuestDiagnostics.com | www.NicholsInstitute.com ______________________________________________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From abilger <@t> wellspan.org Wed Jun 4 09:33:24 2014 From: abilger <@t> wellspan.org (Bilger, Andrea) Date: Wed Jun 4 09:33:58 2014 Subject: [Histonet] HTL program Message-ID: <0258e6074f274d4c81fd07cc80e8b020@BY2PR02MB300.namprd02.prod.outlook.com> I have several techs who used Indiana University's on line course and they are all great techs! Have not been impressed with techs from Harford Community's program. Andrea Bilger, HTL Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ From Nancy_Schmitt <@t> pa-ucl.com Wed Jun 4 09:42:16 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Jun 4 09:42:27 2014 Subject: [Histonet] RE: Histonet Digest, Vol 127, Issue 3 In-Reply-To: <20140603214134.4B98E2D0036@mail.pa-ucl.com> References: <20140603214134.4B98E2D0036@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36D9CAD2DC@PEITHA.wad.pa-ucl.com> Hi Denise- I would HIGHLY recommend the online program at IUPUI - we have had 4 people go through this program including myself. When finished you are ready to sit for the test! It is really well done and they are so helpful along the way. Contact info: Debra M. Wood, Director, Histotechnology Program Clinical Assistant Professor Indiana University School of Medicine Department of Pathology & Laboratory Medicine 350 W. 11th St. ROOM 6002A Indianapolis, IN 46202 Program Office Phone: 317-491-6410 Good Luck! Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA _____________________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise Sent: Tuesday, June 03, 2014 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HTL Online Courses? Hi all! I have been doing massive research on this for past several days but no solid leads. I am wondering about HT/HTL Histology online courses... I live in St. Louis Missouri and they don't offer any histology courses around here - only out of states. I cannot relocate or do 1-2 years program out of state due to my full-time job and family reason. Do anyone know any good Histology online courses that I can take without conflicting with my job? Greatly appericated! Thanks! Denise Smith smith_d@kids.wustl.edu yahoo.com Wed Jun 4 09:40:58 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 4 09:43:47 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: References: Message-ID: <1401892858.49189.YahooMailNeo@web120403.mail.ne1.yahoo.com> You describe a common situation in histology where the pressure to finish the work on time (the TAT) reigns but that does not necessarily mean that quality has to be sacrificed, although sometimes it does. On the other hand, not all histotechs are "created equal" and some have abilities others don't. Manual dexterity is a most and while some can section at the "normal" rate of 24 blocks/hour, other take twice the time, and it seems that you belong to the later group. For what you wrote it seems that your embedding productivity (about 60 blocks/hour) is the "norm" and, again, others are less or more productive. You also point out that you have worked in 3 places in 6-7 years and that is a quite high turnover never conducent to improve your work and that can be hold "against you" in your Resum? when looking for another position. A histology supervisor has to assure the slides are ready for the pathologists on a timely basis so one of the things that have to be done is to identify amongst the staff? "who does what best" meaning who can deliver quality in a timely basis. I think that you, already in your late 20's,?should start trying to answer several questions: 1- why did you in the first place?decided to become a histotech and if that was a "wise" selection? 2- if you are experiencing the same problems in the 3 places where you worked, it seems clear to me that the issue is with you and not with the trade. It will be the same at least in these types of high volume labs where higher productivity are required. If you really like histology and think that you need more training to achieve?sectioning speed, you have to switch to another type of lab. Try to solicit work in a research or university lab where you will have enough time to train properly and where finishing the work "yesterday"?is no usually a concern. Also think that not all histotechs have the same ability or speed. I had supervised along my career as supervisor scores of histotechs and some just cannot?section fast, it is not?within their abilities but can excel in some other tasks. My duty was to detect the task that they could complete best and, without totally frustrating their lives, assign them to those tasks. Also you could try to improve your speed on your own time, and demonstrate that you are also willing to try to improve. So I think that it is time for you to do one of the following: 1- change career 2- change type of lab 3- adapt?and adquire sectioning speed or 4- find amongst the many tasks?that histology provide, the one?where you can excel and at the same find satisfaction. What you cannot do is to keep doing the same and expect to find satisfaction or obtain a different result given your ability. Think hard and honestly what you want to do for the rest of your life that most likely will be long. Ren? J.? On Tuesday, June 3, 2014 4:35 PM, Alpha Histotech wrote: Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out!? So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with.? What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified.? I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jun 4 09:44:03 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 4 09:47:00 2014 Subject: [Histonet] fluorescent scope and slide scanner In-Reply-To: References: Message-ID: <1401893043.69148.YahooMailNeo@web120402.mail.ne1.yahoo.com> Contact a?Leica Microsystems?representative. Ren? J. On Wednesday, June 4, 2014 8:42 AM, "Santiago, Albert" wrote: Hello my colleagues in histoland, We are in the market for a? Slide Scanner and a Fluorescent Microscope, if anyone has any information on any of these products please share with me. Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago@uphs.upenn.edu The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TanyaAbbott <@t> catholichealth.net Wed Jun 4 10:09:25 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Jun 4 10:09:40 2014 Subject: [Histonet] Clearium? Message-ID: <852F7D2C14FB464D80E182B15DB138AF306943FD@CHIEX005.CHI.catholichealth.net> This is a bit more of a Cytology question, but I thought I would survey my Histo friends! We are starting to have bubbles with our PAP slides, and it actually seems to occur after drying, initially after coverslipping (by hand) there appears to be no bubbles. We use Clearium, so we can go directly from alcohol. I am a new manager, and this lab is very adversed to Xylene. Any ideas? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From gu.lang <@t> gmx.at Wed Jun 4 10:35:46 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jun 4 10:36:01 2014 Subject: [Histonet] Pathologists? Message-ID: <000001cf800a$a7ca8750$f75f95f0$@gmx.at> Dear histonetters! Maybe you can help me. We are looking for pathologists for our clinical histolab in Linz, Austria. Anyone (German speaking), who is interested in is welcome to inform him- or herself on this website. http://www.linz.at/akh/10374.asp We have a well equipted, modern histolab (from routine histo till FISH and molecularpathology). Best regards Gudrun From talulahgosh <@t> gmail.com Wed Jun 4 10:44:13 2014 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Wed Jun 4 10:44:18 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF36785FDE@ex07.net.ucsf.edu> <1057490404.2862137.1401845531508.JavaMail.root@comcast.net> Message-ID: As someone who has been in research (basically being a histologist), I can say that there are NO jobs out there for you. The market is saturated with PhDs. Do not leave your job for a research position unless they can guarantee your salary for years. This will be very unlikely, as getting a grant is super hard nowadays. I actually have to be ceritifed to work in a clinical lab, but I know that after 15 years in a lab, I definitely have the skills, just not the certification to be in a clinical lab. I am working in the office now, and in the lab one day a week after having an R01 for ten years and being the lab manager in a research lab. I'm going to get certification in case this office/lab thing doesn't work out in a few years. I wish there was more money in science but there isn't. So the main point is, either get some skills, or go a different path. Research is not where it's at right now. Although, I am assuming you're in the US, this might not be the case in other countries. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Jun 4, 2014 at 7:45 AM, joelle weaver wrote: > Yes thanks for the perspective. I have a bias towards my own experience, > and this seems to be good advice. I work in a molecular based lab now and > they are very unaware of what it typically is like in a clinical > histopathology lab. Good to point other environments are out there that are > clinical, and also that research in general can be very different than > clinical settings. Some people are just more suited to certain environments > over the other. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > Date: Wed, 4 Jun 2014 01:32:11 +0000 > From: koellingr@comcast.net > To: joelleweaver@hotmail.com > CC: timothy.morken@ucsfmedctr.org; optimusprimehistotech@hotmail.com; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Should I leave histology world > > Alpha Histotech- > > I'll put in my few words even though I'm not active anymore and possibly > from different perspective. But also using a few assumptions and if my > assumptions are wrong then the rest of what I say is probably meaningless. > Not ID?ng your e-mail address but if you've worked 3 jobs nightshift > including a large reference lab, do you live near a big city? And if so is > it a city close to a college or university. > > Research histology should not be overlooked. You will find many molecular > or other such non-histo labs that actually do some or even a lot of > histology by non-histology personnel or lab workers. Sometimes it is OK, > sometimes even great. Sometimes, and I witnessed it, it is at an > embarrassing histo level. I can walk up or down university hallways and > see a "genetics lab" or some other "molecular lab" and see a microtome or > cryostat in there. Sometimes those PI's will send histo work to a core > lab. Sometimes they don't want to pay per block so do it (and staining and > IHC and FISH) themselves. Someone with even minimal wide-ranging histo > experience might be welcomed. > > No timed block cutting counts. Learn some immunology, genetics, molecular > techniques, comparative medicine, physiology, etc, etc along the way. Many > places even pay for college level courses while employed there. > > Just a thought if you are near that kind of area. > > Ray in Seattle > > > > > > From: "joelle weaver" > To: "Timothy Morken" , "Alpha Histotech" < > optimusprimehistotech@hotmail.com>, histonet@lists.utsouthwestern.edu > Sent: Tuesday, June 3, 2014 5:55:09 PM > Subject: RE: [Histonet] Should I leave histology world > > > It would be a shame to get discouraged now after all the time you have > already put into histology. If you still want to work in histology, I might > suggest you try to have a conversation with a manager, supervisor or lead > tech and see if they are willing to support you. Tell them you want to > spend more time cuting to be able to section with high quality at the rate > that works for their productivity standards. If you present it as a > win-win proposition, see what resources, people and time they are willing > to "chip in" to help get where they would like you to be. Make some metric > or rate to achieve in microtomy your goal for the year, and put it into > writing ( good for all goals:). > Or if that is too uncomfortable , approach someone individually whose > microtomy skills you admire , and see if they are willing to provide some > tips and guidance off work time. > > I also went through a NAACLS program. Still at my first "real" histology > job , the realization that this was the actual training became apparent > very quickly. I had moments of exhaustion and feeling overwhelmed, but I > now feel I was also fortunate to work initially at a pretty high volume > place. It was a great "breaking in" for embedding and microtomy. Luckily > there were also some experienced techs there who saw how much I wanted to > learn, and were willing to help me get better. The "constructive" > criticism stung sometimes, but they did me a huge service. But believe me, > not everyone was helpful or supportive along the way. Try to ignore those > kind of people as much as possible. And I still get criticized sometimes, > make mistakes, and I still have more to learn. > > But here are a couple of other options for you to consider before you > decide to leave, and what I did to get more experience when in your > situation more quickly; > > Take on a second histology job that targets specific skills, tissue, or > techniques you want more experience in. Believe me I have been criticized > and misunderstood for this choice s well many times, but personally I do > not regret any of those experiences now. > > I also feel that small labs are nice to build well rounded skills since > you are usually more of a "jack of all trades" and have less tendency to do > one task over your whole shift from day to day. Sometimes you just have to > identify the environment that is the right fit for you. > > Best of luck to you- and let us know how things turn out! > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: Timothy.Morken@ucsfmedctr.org > > To: optimusprimehistotech@hotmail.com; histonet@lists.utsouthwestern.edu > > Date: Tue, 3 Jun 2014 22:51:31 +0000 > > Subject: RE: [Histonet] Should I leave histology world > > CC: > > > > Alpha, it is clear to me, after 30+ years in the field, that some are > born with the ability to cut fast AND do well at it. The rest of us just > have to work harder at developing that skill. But it does take bench time > to do it. A recent cache is that it takes 10,000 hours to become an > absolute expert at something - that's about 5 years full time work. And > that's just one skill. > > > > It sounds like you need some good teachers (ie, those who like to teach > and like having their students do well). That would be the highest priority > if you want to stay in the field as a bench tech. > > > > If the factory job isn't working out why not look for a smaller lab in > which you can get more extensive experience? I really value the fact that > spent my first 11 years in a 4- person lab in which we did everything from > grossing to histo to immunos to EM. It may pay less initially but may add > more value to your lifetime career. > > > > > > Tim Morken > > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies > > UC San Francisco Medical Center > > San Francisco, CA > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alpha Histotech > > Sent: Tuesday, June 03, 2014 1:35 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Should I leave histology world > > > > Hi everyone, > > > > I wouldn't give too much detail information as the histology world is > very small and everyone knows everyone. > > > > I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. > I went to a NAACLS school and have a Associate in Science in Histology. In > the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard > shifts. The first place I worked for was Quest Diagnostics and I did a good > 3 yrs. The other 2 places I won't mention and I currently still have a > histology job. My problem is all the places I worked were factory style lab > work and they all did derm work. In my career I really only embedded most > of the time. I did occasional other stuff like special stains both by hand > and using Dako Artisan and other things like cytology cytospin. But I never > got to develop in cutting. My first job in quest..I maybe cutted one time > for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job > put me to cut the last 2 months (full 8hrs) I was working there. My current > job I have been cutting since April 2014 ( but only 2-3hrs in the day and > then I embed, I have been here now 1 yr, I was embedding most of the time > before th cutting started). I was told by my director I need to speed up in > cutting because corporate is asking why I am not increasing in speed. And > if I don't speed up eventually then they will have to demote me to a lab > aid and give me a pay cut. (where I work and the state I work in they have > lab aids doing alot of stuff without being certified, it wasn't like that > in the other state I am original from as you have to be state licensed and > ascp) I sometimes laugh inside my head because before my director hired me > I told him I don't have alot experience in cutting. > > > > Now everywhere I have gone...speed is the name of the game. They say > they care about quality but in the end if you can't put up then you will be > put out! So I am just thinking I should just get out of histology world > all together. Every where I have worked unfortunately have management who > believe quantity over quality. OR Do you guys think I need more time > cutting to develop speed? Beforehand I did need a little learning curve to > cut and I have gotten through that now. It's just the speed that is killing > me. And I also see if anyone at my work detours me for any reason like for > example data entry person from front desk ask for missing gross dictation, > then that lost time is very hard to recover as I am not soooo fast to > recover. I feel I may have to become very rude(not help) with everyone I > work around in order to stay glued to my seat when I am cutting my blocks. > One thing I want to say also...until this day I never been written up for > quality issues and I never lost any tissue while embedding. Embedding I am > fast as most histotech (1 block a min or most times 30-45 secs 1 block) > with proper embedding techniques demonstrated (tissue on same plane, tissue > embedded with proper orientation and follow any other necessary embedding > instructions. ) I just feel I haven't done my time in cutting as I did in > embedding to become a fast cutter. I don't know if its because of working > in a derm lab that management won't wait too long for you to develop like > maybe a hospital lab may do. I was also thinking to find another histo job > but not mention any of my experience so expectation won't be so high and I > can get more time to develop. All of this also causes alot of stress and > anxiety as it gets hard to coop with. What do you guys think and how I > should go about with this. I am also not limited to histology. I have > expertise in 2 other major fields that I wont mention because I don't want > to be identified. I am also in my late 20's. Thanks for reading my post > and I await your opinions as some of you all are veterans in the field of > histology. > > > > Thank you > > Alpha Histotech (ASCP HT) > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken <@t> ucsfmedctr.org Wed Jun 4 10:46:57 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Jun 4 10:47:07 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: <1401892858.49189.YahooMailNeo@web120403.mail.ne1.yahoo.com> References: <1401892858.49189.YahooMailNeo@web120403.mail.ne1.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF367860D8@ex07.net.ucsf.edu> Rene is right that everyone needs to find what they are good at. We had a guy who was so-so in cutting speed and always getting flack for not doing enough. And had such a hard time coordinating specials and immunos that he just slowed things down. Then we started doing Mega blocks of whole mount eyes and prostate and it turns out this guy is a savant at cutting large blocks. The pathologists were raving about how good the sections were. He had a job as long as he wanted in that lab just for that! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 04, 2014 7:41 AM To: Alpha Histotech; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Should I leave histology world You describe a common situation in histology where the pressure to finish the work on time (the TAT) reigns but that does not necessarily mean that quality has to be sacrificed, although sometimes it does. On the other hand, not all histotechs are "created equal" and some have abilities others don't. Manual dexterity is a most and while some can section at the "normal" rate of 24 blocks/hour, other take twice the time, and it seems that you belong to the later group. For what you wrote it seems that your embedding productivity (about 60 blocks/hour) is the "norm" and, again, others are less or more productive. You also point out that you have worked in 3 places in 6-7 years and that is a quite high turnover never conducent to improve your work and that can be hold "against you" in your Resum? when looking for another position. A histology supervisor has to assure the slides are ready for the pathologists on a timely basis so one of the things that have to be done is to identify amongst the staff? "who does what best" meaning who can deliver quality in a timely basis. I think that you, already in your late 20's,?should start trying to answer several questions: 1- why did you in the first place?decided to become a histotech and if that was a "wise" selection? 2- if you are experiencing the same problems in the 3 places where you worked, it seems clear to me that the issue is with you and not with the trade. It will be the same at least in these types of high volume labs where higher productivity are required. If you really like histology and think that you need more training to achieve?sectioning speed, you have to switch to another type of lab. Try to solicit work in a research or university lab where you will have enough time to train properly and where finishing the work "yesterday"?is no usually a concern. Also think that not all histotechs have the same ability or speed. I had supervised along my career as supervisor scores of histotechs and some just cannot?section fast, it is not?within their abilities but can excel in some other tasks. My duty was to detect the task that they could complete best and, without totally frustrating their lives, assign them to those tasks. Also you could try to improve your speed on your own time, and demonstrate that you are also willing to try to improve. So I think that it is time for you to do one of the following: 1- change career 2- change type of lab 3- adapt?and adquire sectioning speed or 4- find amongst the many tasks?that histology provide, the one?where you can excel and at the same find satisfaction. What you cannot do is to keep doing the same and expect to find satisfaction or obtain a different result given your ability. Think hard and honestly what you want to do for the rest of your life that most likely will be long. Ren? J.? On Tuesday, June 3, 2014 4:35 PM, Alpha Histotech wrote: Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out!? So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with.? What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified.? I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Jun 4 11:06:01 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 4 11:06:07 2014 Subject: [Histonet] RE: fluorescent scope and slide scanner In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05C9A@SBS2K8.premierlab.local> Albert It all depends on your through put and how many slides you need to scan daily. There are so many other things you need to consider prior to purchasing a scanner. If you go to the DPA website you can get a lot on information on digital pathology www.digitalpathologyassociation.org The larger vendors such as Leica/Aperio, Olympus, Philips, GE, Ventana will have different solutions and packages for what you may need to include storage and database, reporting, image analysis, etc. You need to think about how you want to use the scanner prior to purchasing one. I have contact information for most of the vendors so if you want me to provide that to you I can. Now I'm going to give a shameless plug - Jesus Elin, Bill DeSalvo and myself will be giving an all day workshop on this very topic at NSH in Austin it's on Saturday. If you can't make it to that meeting then the DPA has Pathology Visions in October you would be able to spend time with a lot of the vendors that are in this digital pathology space and in addition to that Bill DeSalvo and I will also presenting on this topic at the Region VII meeting in Phoenix in July. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santiago, Albert Sent: Wednesday, June 04, 2014 6:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fluorescent scope and slide scanner Hello my colleagues in histoland, We are in the market for a Slide Scanner and a Fluorescent Microscope, if anyone has any information on any of these products please share with me. Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago@uphs.upenn.edu The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi <@t> nrh-ok.com Wed Jun 4 11:20:32 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Wed Jun 4 11:20:38 2014 Subject: [Histonet] HER2 by IHC- Fixation Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82214D@MAIL.nrhnt.nrh-ok.com> Hi Histonetters, I hope you guys are doing great. Please I wanted to confirm whether it is true that the CAP has changed the HER2 Fixation time from 6 - 48 hours to 6 - 72 hours. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From Joyce.Weems <@t> emoryhealthcare.org Wed Jun 4 11:22:52 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Jun 4 11:23:03 2014 Subject: [Histonet] HER2 by IHC- Fixation In-Reply-To: <04EE4F75BB5FB246ADB68D69B74604438E3B82214D@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B74604438E3B82214D@MAIL.nrhnt.nrh-ok.com> Message-ID: That is true. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Wednesday, June 04, 2014 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 by IHC- Fixation Hi Histonetters, I hope you guys are doing great. Please I wanted to confirm whether it is true that the CAP has changed the HER2 Fixation time from 6 - 48 hours to 6 - 72 hours. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From koellingr <@t> comcast.net Wed Jun 4 11:50:05 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jun 4 11:50:24 2014 Subject: [Histonet] leaving histology question research is still an option In-Reply-To: <1914442437.3208506.1401899215775.JavaMail.root@comcast.net> Message-ID: <1831821306.3228072.1401900605181.JavaMail.root@comcast.net> Alpha Histotech, ? wanted to be sure that I did?NOT tell you to drop everything in life to look to research exclusively.? So I cut and pasted this from my original message "Research histology should not be overlooked I stand by that statement. ? I agree with Emily that funding in research is (stupidly for this nation) difficult.? But it is not zero.? PhD's do NOT saturate histotech jobs in research labs.? There might be a few, maybe some, maybe a lot in some places but not all.? No one, even a clinical lab, will guarantee that job for years and years.? I know many histotech/non-histotech "techs" who have been through 5-6 different more molecular labs in the same building for?over 30 years.? So have 30 years seniority in the system.? Grant runs out and you move to a different lab (and is way easier having had made connections in first lab).? I made 3x in research industry then I could ever have made in clinical histo lab.? Is not for everyone but also not something to dismiss as hopeless.? If you ever do research, you will find that "N of 1" is not reliable to base everything and every decision on.? Best of luck.? Search for those options several others have given but don't dismiss my suggestion outright. ? Ray in Seattle (histotech from 1960's who could only retire now because of Research histology) From TanyaAbbott <@t> catholichealth.net Wed Jun 4 12:01:06 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Jun 4 12:01:16 2014 Subject: [Histonet] Acid Cleaned glassware Message-ID: <852F7D2C14FB464D80E182B15DB138AF30694589@CHIEX005.CHI.catholichealth.net> We just acquired a brand new laboratory microwave to replace ours from the 1980s,..........so we are optimizing our GMS stain. Currently we would let the glassware sit in Chromic acid for 1 hr to chemically clean the glassware before staining. Now we are using the CAP required polypropylene vented microwave containers. Can we skip the chromic Acid step? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From rjbuesa <@t> yahoo.com Wed Jun 4 12:07:12 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 4 12:10:26 2014 Subject: [Histonet] Acid Cleaned glassware In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF30694589@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF30694589@CHIEX005.CHI.catholichealth.net> Message-ID: <1401901632.72652.YahooMailNeo@web120406.mail.ne1.yahoo.com> Venting the MW oven is just a safety issue and has nothing to do with the cleaning of the glassware and the?quality of the staining procedure. Ren? J.? On Wednesday, June 4, 2014 1:01 PM, "Abbott, Tanya" wrote: We just acquired a brand new laboratory microwave to replace ours from the 1980s,..........so we are optimizing our GMS stain. Currently we would let the glassware sit in Chromic acid for 1 hr to chemically clean the glassware before staining. Now we are using the CAP required polypropylene vented microwave containers. Can we skip the chromic Acid step? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph? 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Jun 4 12:19:00 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Jun 4 12:19:09 2014 Subject: [Histonet] RE: leaving histology---research still an option Message-ID: <9F3CFEE76E51B64991C7485270890B40498C8A38@EX5.lj.gnf.org> Thank you Ray for your perspective. I wholeheartedly agree with you. A few (maybe more than a few) years back I wrote an article for NSH on transitioning from Clinical to Research. You'll find it here: http://www.nsh.org/content/transitioning-clinical-research In most cases speed is not the goal. Instead a combination of efficiency, consistency and excellence is expected. If what I have written doesn't appeal to you, then please keep your other options open. Best wishes, Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From kim-lake <@t> uiowa.edu Wed Jun 4 12:30:33 2014 From: kim-lake <@t> uiowa.edu (Lake, Kim S) Date: Wed Jun 4 12:30:41 2014 Subject: [Histonet] slide drying/curing before filing Message-ID: According to the Histonet archives, this hasn't been discussed for almost a decade. How are labs drying/curing their slides before they are filed? We are a small oral pathology laboratory and we hand-coverslip using Richard Allen mounting medium and glass coverslips. After cases are signed out slides are placed in their cardboard folders in a 37C oven for at least a week, then are filed in metal slides drawers. This works perfectly well, but we would like to simplify this, if possible. A search of the archive showed a great deal of variation in slide drying protocol, I'm interested in hearing what everyone else is up to. Of course, I'm hoping to get lots of people saying that they file all of last week's room temp slides on Monday morning and it works like a charm! Thanks! Kim Lake, MLS (ASCP)CM Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 From tbraud <@t> holyredeemer.com Wed Jun 4 12:30:02 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Jun 4 12:31:38 2014 Subject: [Histonet] RE:Change in Her2 Neu Fixation In-Reply-To: <20140604161600.308EB1E807E@trendmess-svr.holyredeemer.local> References: <20140604161600.308EB1E807E@trendmess-svr.holyredeemer.local> Message-ID: As of the 4/2014 revision, CAP extended the fixation time to 72 hours. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 10. HER2 by IHC- Fixation (Adesupo, Adesuyi (Banjo)) 11. RE: HER2 by IHC- Fixation (Weems, Joyce K.) Message: 10 Date: Wed, 4 Jun 2014 11:20:32 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] HER2 by IHC- Fixation Hi Histonetters, I hope you guys are doing great. Please I wanted to confirm whether it is true that the CAP has changed the HER2 Fixation time from 6 - 48 hours to 6 - 72 hours Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 That is true. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From tony.auge <@t> gmail.com Wed Jun 4 12:58:48 2014 From: tony.auge <@t> gmail.com (Tony Auge) Date: Wed Jun 4 12:58:54 2014 Subject: [Histonet] Microtome In-Reply-To: References: Message-ID: Of the three microtomes that you listed I would highly recommend the Thermo Shandon Finesse. It's of superior quality than the Microms I have used. Cutting is excellent and the fly wheel action is light yet fluid. You do have to oil it manually once a month but it's easy and you can clean it out in the process and keep it in good operating condition. I haven't used the Titan 5000 but I have used slides from Tanner that were of poor quality and I ended up returning them. I'm cutting on a used Leica 2125 right now. It is functional but it does skip when facing and block orientation is terrible. Getting used lab equipment is always a gamble so make sure your supplier is pleasant to deal with and offers a warranty. Not sure what your budget is but a new Finesse isn't too expensive for a new microtome and I'm sure you will be very happy with it. Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com From Joyce.Weems <@t> emoryhealthcare.org Wed Jun 4 13:35:51 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Jun 4 13:36:25 2014 Subject: [Histonet] RE: slide drying/curing before filing In-Reply-To: References: Message-ID: We use quick drying Richard Allen Mounting Media - 22 050 102, purchased through Fisher Healthcare. We file the next day!! Glass coverslips... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S Sent: Wednesday, June 04, 2014 1:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide drying/curing before filing According to the Histonet archives, this hasn't been discussed for almost a decade. How are labs drying/curing their slides before they are filed? We are a small oral pathology laboratory and we hand-coverslip using Richard Allen mounting medium and glass coverslips. After cases are signed out slides are placed in their cardboard folders in a 37C oven for at least a week, then are filed in metal slides drawers. This works perfectly well, but we would like to simplify this, if possible. A search of the archive showed a great deal of variation in slide drying protocol, I'm interested in hearing what everyone else is up to. Of course, I'm hoping to get lots of people saying that they file all of last week's room temp slides on Monday morning and it works like a charm! Thanks! Kim Lake, MLS (ASCP)CM Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From aobrien88 <@t> comcast.net Wed Jun 4 13:39:22 2014 From: aobrien88 <@t> comcast.net (aobrien88@comcast.net) Date: Wed Jun 4 13:39:31 2014 Subject: [Histonet] IHC patient reports In-Reply-To: <225629961.3504669.1401907081943.JavaMail.root@comcast.net> Message-ID: <657610021.3505831.1401907162834.JavaMail.root@comcast.net> How long is everyone keeping the IHC reports that are printed off after the run is completed? ANP.22660 states that Batch control records must be retained to 2 years. Is that also the patient? Thanks From Timothy.Morken <@t> ucsfmedctr.org Wed Jun 4 13:45:20 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Jun 4 13:45:29 2014 Subject: [Histonet] RE: IHC patient reports In-Reply-To: <657610021.3505831.1401907162834.JavaMail.root@comcast.net> References: <225629961.3504669.1401907081943.JavaMail.root@comcast.net> <657610021.3505831.1401907162834.JavaMail.root@comcast.net> Message-ID: <761E2B5697F795489C8710BCC72141FF36786222@ex07.net.ucsf.edu> The patient results are in the final report. The 2 year requirement is for QC documents only. Tim Morken Supervisor,Hisotlogy, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of aobrien88@comcast.net Sent: Wednesday, June 04, 2014 11:39 AM To: histonet Subject: [Histonet] IHC patient reports How long is everyone keeping the IHC reports that are printed off after the run is completed? ANP.22660 states that Batch control records must be retained to 2 years. Is that also the patient? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jpiche <@t> wtbyhosp.org Wed Jun 4 13:48:00 2014 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Wed Jun 4 13:48:06 2014 Subject: [Histonet] CAP Question CYP.04300 Daily QC Message-ID: <631955447A364B45B9458D2905635110BA0B85D5@WIN08-MBX-02.wtbyhosp.org> Hi Everyone, I am asking a question for our Cytology department about CYP.04300 on the CAP checklist, in regards to daily QC. It states the technical quality of the preparations should be checked daily and includes checking all stains for predicted staining characteristics each day of use. This check should include preparations performed in the lab such as cytospins, cell blocks, and liquid based automated preparations. Is the question asking to document evidence of the technical quality of the stain or the technical quality in the preparation of the slide, ie smear, cytospin, etc? Or does it want both? Our cytology department uses several different protocols and with several different slide preparations so they are wondering if they are supposed to run a control every day for every single type of slide prep or just every stain that is used? We would be interested in knowing what everyone else does. Thank you and have a great day! Jessica Piche,HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From skiousis <@t> med.wayne.edu Wed Jun 4 13:53:21 2014 From: skiousis <@t> med.wayne.edu (Kiousis, Sam) Date: Wed Jun 4 13:53:30 2014 Subject: [Histonet] microglia antibodies Message-ID: <18E6F3EDA42AB04280E9C218D20832361C736749@MED-CORE07B.med.wayne.edu> I have been trying to get anitbodies for microglia (CD68, CD11b, IBA-1) to work using IHC and IF (regular, co-localization, even TSA amplification) on slides from frozen tissue that has been fixed in PFA and embedded in OCT with mixed crappy results. Does anyone have any suggestions or protocols? Thanks in advance Sam Kiousis Karmanos Cancer Institute skiousis@med.wayne.edu From JPitts <@t> dermatologyprofessionals.com Wed Jun 4 13:54:45 2014 From: JPitts <@t> dermatologyprofessionals.com (Jackie Pitts) Date: Wed Jun 4 13:54:52 2014 Subject: [Histonet] Great Job Opprotunity Message-ID: <85F6442EC5759B48900C8454E873373102280E4FF78B@SBS01.dermpro.local> Hello all! I am posting a great opportunity for a confident and experienced histotech. It is located in Baxter, MN. You would be working for Dr. Lundstrom at Dermatology Professionals. The job is to for the most part run the histology lab. Right now this is my job and I am leaving only because I found a job nearer to my family. You need to have experience in histology as you will be working alone and need to be able to handle the day to day situations. Grossing experience is also required as you will be grossing the skin shaves, punches and excisions. You will then be processing, embedding, cutting and staining the slides. Must also be ASCP certified. MOHS experience would be a plus too, but not required. Please contact me with a resume or any questions regarding this position. I am trying to help them get going on replacing me before I leave them at the end of the month. :) Jackie Heitz, HT(ASCP)CM 218-454-3520 Dermatology Professionals, PA Baxter, MN 56425 From amurvosh <@t> advancederm.net Wed Jun 4 13:57:56 2014 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Wed Jun 4 13:58:03 2014 Subject: [Histonet] Practice tissue? In-Reply-To: <03217A44-2EE4-418A-BF1F-220BB4D77D1B@kent.edu> References: <03217A44-2EE4-418A-BF1F-220BB4D77D1B@kent.edu> Message-ID: <4AD6A4E531E8C943A730559B6B81DF07DA9264@dc.Advancederm.net> Contact your local labs and Hospitals, you could just use some already embedded and cut tissue that is about to be disposed of as long as there is no patient info on the blocks it shouldn't be a problem. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Waugh Sent: Tuesday, June 03, 2014 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Practice tissue? Hello histonet, I was a member a number of years ago, but had gotten out of doing much histology (I was never a pro) for awhile.... but I seem to be back at it. I was wondering if people might have some ideas on a good "practice tissue", one that I could embed, section, and stain (H&E) to both practice my sectioning, and check my staining protocol, etc. I really need to be sectioning some decalcified teeth, and have had some luck, but I would really like to do some more practice on an "easy to section tissue" (other than just a blank block of paraffin) that I can also test my staining on. I have accesses to a tissue processor. Is there anything I can just pick up at the grocery store? Thanks, David David A. Waugh, Ph.D. Research Assistant Department of Anatomy and Neurobiology NEOMED 4209 State Route 44 Rootstown, OH 44272 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Jun 4 14:05:28 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 4 14:05:33 2014 Subject: [Histonet] RE: microglia antibodies In-Reply-To: <18E6F3EDA42AB04280E9C218D20832361C736749@MED-CORE07B.med.wayne.edu> References: <18E6F3EDA42AB04280E9C218D20832361C736749@MED-CORE07B.med.wayne.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05CAC@SBS2K8.premierlab.local> What species are you looking to stain? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kiousis, Sam Sent: Wednesday, June 04, 2014 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microglia antibodies I have been trying to get anitbodies for microglia (CD68, CD11b, IBA-1) to work using IHC and IF (regular, co-localization, even TSA amplification) on slides from frozen tissue that has been fixed in PFA and embedded in OCT with mixed crappy results. Does anyone have any suggestions or protocols? Thanks in advance Sam Kiousis Karmanos Cancer Institute skiousis@med.wayne.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carol.Epp <@t> saskatoonhealthregion.ca Wed Jun 4 14:47:06 2014 From: Carol.Epp <@t> saskatoonhealthregion.ca (Epp, Carol SktnHR) Date: Wed Jun 4 14:47:17 2014 Subject: [Histonet] How to ask a question on Histonet? In-Reply-To: <850b8c50-9181-4808-9ff4-fefb8d4ab62c@EX-EDG-V2.sktnhr.ca> References: <850b8c50-9181-4808-9ff4-fefb8d4ab62c@EX-EDG-V2.sktnhr.ca> Message-ID: <4408321938F9704A999D4837C92E4D94C4D6C841@EX-MBD-R1.sktnhr.ca> I would like to ask a question:Headline-MICROSCOPIC CHECKS- "What slides do you microscopically check before sending out to the pathologist?" carol.epp@saskatoonhealthregion.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, June 04, 2014 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. HTL program (Bilger, Andrea) 2. RE: Histonet Digest, Vol 127, Issue 3 (Nancy Schmitt) 3. Re: Should I leave histology world (Rene J Buesa) 4. Re: fluorescent scope and slide scanner (Rene J Buesa) 5. Clearium? (Abbott, Tanya) 6. Pathologists? (Gudrun Lang) 7. Re: Should I leave histology world (Emily Brown) 8. RE: Should I leave histology world (Morken, Timothy) 9. RE: fluorescent scope and slide scanner (Elizabeth Chlipala) 10. HER2 by IHC- Fixation (Adesupo, Adesuyi (Banjo)) 11. RE: HER2 by IHC- Fixation (Weems, Joyce K.) 12. leaving histology question research is still an option (koellingr@comcast.net) ---------------------------------------------------------------------- Message: 1 Date: Wed, 4 Jun 2014 14:33:24 +0000 From: "Bilger, Andrea" Subject: [Histonet] HTL program To: "histonet@lists.utsouthwestern.edu" Message-ID: <0258e6074f274d4c81fd07cc80e8b020@BY2PR02MB300.namprd02.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" I have several techs who used Indiana University's on line course and they are all great techs! Have not been impressed with techs from Harford Community's program. Andrea Bilger, HTL Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ ------------------------------ Message: 2 Date: Wed, 4 Jun 2014 14:42:16 +0000 From: Nancy Schmitt Subject: [Histonet] RE: Histonet Digest, Vol 127, Issue 3 To: "histonet@lists.utsouthwestern.edu" Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36D9CAD2DC@PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Hi Denise- I would HIGHLY recommend the online program at IUPUI - we have had 4 people go through this program including myself. When finished you are ready to sit for the test! It is really well done and they are so helpful along the way. Contact info: Debra M. Wood, Director, Histotechnology Program Clinical Assistant Professor Indiana University School of Medicine Department of Pathology & Laboratory Medicine 350 W. 11th St. ROOM 6002A Indianapolis, IN 46202 Program Office Phone: 317-491-6410 Good Luck! Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA _____________________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise Sent: Tuesday, June 03, 2014 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HTL Online Courses? Hi all! I have been doing massive research on this for past several days but no solid leads. I am wondering about HT/HTL Histology online courses... I live in St. Louis Missouri and they don't offer any histology courses around here - only out of states. I cannot relocate or do 1-2 years program out of state due to my full-time job and family reason. Do anyone know any good Histology online courses that I can take without conflicting with my job? Greatly appericated! Thanks! Denise Smith smith_d@kids.wustl.edu Subject: Re: [Histonet] Should I leave histology world To: Alpha Histotech , "histonet@lists.utsouthwestern.edu" Message-ID: <1401892858.49189.YahooMailNeo@web120403.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You describe a common situation in histology where the pressure to finish the work on time (the TAT) reigns but that does not necessarily mean that quality has to be sacrificed, although sometimes it does. On the other hand, not all histotechs are "created equal" and some have abilities others don't. Manual dexterity is a most and while some can section at the "normal" rate of 24 blocks/hour, other take twice the time, and it seems that you belong to the later group. For what you wrote it seems that your embedding productivity (about 60 blocks/hour) is the "norm" and, again, others are less or more productive. You also point out that you have worked in 3 places in 6-7 years and that is a quite high turnover never conducent to improve your work and that can be hold "against you" in your Resum? when looking for another position. A histology supervisor has to assure the slides are ready for the pathologists on a timely basis so one of the things that have to be done is to identify amongst the staff? "who does what best" meaning who can deliver quality in a timely basis. I think that you, already in your late 20's,?should start trying to answer several questions: 1- why did you in the first place?decided to become a histotech and if that was a "wise" selection? 2- if you are experiencing the same problems in the 3 places where you worked, it seems clear to me that the issue is with you and not with the trade. It will be the same at least in these types of high volume labs where higher productivity are required. If you really like histology and think that you need more training to achieve?sectioning speed, you have to switch to another type of lab. Try to solicit work in a research or university lab where you will have enough time to train properly and where finishing the work "yesterday"?is no usually a concern. Also think that not all histotechs have the same ability or speed. I had supervised along my career as supervisor scores of histotechs and some just cannot?section fast, it is not?within their abilities but can excel in some other tasks. My duty was to detect the task that they could complete best and, without totally frustrating their lives, assign them to those tasks. Also you could try to improve your speed on your own time, and demonstrate that you are also willing to try to improve. So I think that it is time for you to do one of the following: 1- change career 2- change type of lab 3- adapt?and adquire sectioning speed or 4- find amongst the many tasks?that histology provide, the one?where you can excel and at the same find satisfaction. What you cannot do is to keep doing the same and expect to find satisfaction or obtain a different result given your ability. Think hard and honestly what you want to do for the rest of your life that most likely will be long. Ren? J.? On Tuesday, June 3, 2014 4:35 PM, Alpha Histotech wrote: Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out!? So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with.? What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified.? I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 4 Jun 2014 07:44:03 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] fluorescent scope and slide scanner To: "Santiago, Albert" , "histonet@lists.utsouthwestern.edu" Message-ID: <1401893043.69148.YahooMailNeo@web120402.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Contact a?Leica Microsystems?representative. Ren? J. On Wednesday, June 4, 2014 8:42 AM, "Santiago, Albert" wrote: Hello my colleagues in histoland, We are in the market for a? Slide Scanner and a Fluorescent Microscope, if anyone has any information on any of these products please share with me. Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago@uphs.upenn.edu The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 4 Jun 2014 15:09:25 +0000 From: "Abbott, Tanya" Subject: [Histonet] Clearium? To: "histonet@lists.utsouthwestern.edu" Message-ID: <852F7D2C14FB464D80E182B15DB138AF306943FD@CHIEX005.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" This is a bit more of a Cytology question, but I thought I would survey my Histo friends! We are starting to have bubbles with our PAP slides, and it actually seems to occur after drying, initially after coverslipping (by hand) there appears to be no bubbles. We use Clearium, so we can go directly from alcohol. I am a new manager, and this lab is very adversed to Xylene. Any ideas? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 6 Date: Wed, 4 Jun 2014 17:35:46 +0200 From: "Gudrun Lang" Subject: [Histonet] Pathologists? To: Message-ID: <000001cf800a$a7ca8750$f75f95f0$@gmx.at> Content-Type: text/plain; charset="us-ascii" Dear histonetters! Maybe you can help me. We are looking for pathologists for our clinical histolab in Linz, Austria. Anyone (German speaking), who is interested in is welcome to inform him- or herself on this website. http://www.linz.at/akh/10374.asp We have a well equipted, modern histolab (from routine histo till FISH and molecularpathology). Best regards Gudrun ------------------------------ Message: 7 Date: Wed, 4 Jun 2014 11:44:13 -0400 From: Emily Brown Subject: Re: [Histonet] Should I leave histology world Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 As someone who has been in research (basically being a histologist), I can say that there are NO jobs out there for you. The market is saturated with PhDs. Do not leave your job for a research position unless they can guarantee your salary for years. This will be very unlikely, as getting a grant is super hard nowadays. I actually have to be ceritifed to work in a clinical lab, but I know that after 15 years in a lab, I definitely have the skills, just not the certification to be in a clinical lab. I am working in the office now, and in the lab one day a week after having an R01 for ten years and being the lab manager in a research lab. I'm going to get certification in case this office/lab thing doesn't work out in a few years. I wish there was more money in science but there isn't. So the main point is, either get some skills, or go a different path. Research is not where it's at right now. Although, I am assuming you're in the US, this might not be the case in other countries. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Jun 4, 2014 at 7:45 AM, joelle weaver wrote: > Yes thanks for the perspective. I have a bias towards my own > experience, and this seems to be good advice. I work in a molecular > based lab now and they are very unaware of what it typically is like > in a clinical histopathology lab. Good to point other environments are > out there that are clinical, and also that research in general can be > very different than clinical settings. Some people are just more > suited to certain environments over the other. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > Date: Wed, 4 Jun 2014 01:32:11 +0000 > From: koellingr@comcast.net > To: joelleweaver@hotmail.com > CC: timothy.morken@ucsfmedctr.org; optimusprimehistotech@hotmail.com; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Should I leave histology world > > Alpha Histotech- > > I'll put in my few words even though I'm not active anymore and > possibly from different perspective. But also using a few assumptions > and if my assumptions are wrong then the rest of what I say is probably meaningless. > Not ID??ng your e-mail address but if you've worked 3 jobs nightshift > including a large reference lab, do you live near a big city? And if > so is it a city close to a college or university. > > Research histology should not be overlooked. You will find many > molecular or other such non-histo labs that actually do some or even a > lot of histology by non-histology personnel or lab workers. Sometimes > it is OK, sometimes even great. Sometimes, and I witnessed it, it is > at an embarrassing histo level. I can walk up or down university > hallways and see a "genetics lab" or some other "molecular lab" and > see a microtome or cryostat in there. Sometimes those PI's will send > histo work to a core lab. Sometimes they don't want to pay per block > so do it (and staining and IHC and FISH) themselves. Someone with > even minimal wide-ranging histo experience might be welcomed. > > No timed block cutting counts. Learn some immunology, genetics, > molecular techniques, comparative medicine, physiology, etc, etc along > the way. Many places even pay for college level courses while employed there. > > Just a thought if you are near that kind of area. > > Ray in Seattle > > > > > > From: "joelle weaver" > To: "Timothy Morken" , "Alpha > Histotech" < optimusprimehistotech@hotmail.com>, > histonet@lists.utsouthwestern.edu > Sent: Tuesday, June 3, 2014 5:55:09 PM > Subject: RE: [Histonet] Should I leave histology world > > > It would be a shame to get discouraged now after all the time you have > already put into histology. If you still want to work in histology, I > might suggest you try to have a conversation with a manager, > supervisor or lead tech and see if they are willing to support you. > Tell them you want to spend more time cuting to be able to section > with high quality at the rate that works for their productivity > standards. If you present it as a win-win proposition, see what > resources, people and time they are willing to "chip in" to help get > where they would like you to be. Make some metric or rate to achieve > in microtomy your goal for the year, and put it into writing ( good for all goals:). > Or if that is too uncomfortable , approach someone individually whose > microtomy skills you admire , and see if they are willing to provide > some tips and guidance off work time. > > I also went through a NAACLS program. Still at my first "real" > histology job , the realization that this was the actual training > became apparent very quickly. I had moments of exhaustion and feeling > overwhelmed, but I now feel I was also fortunate to work initially at a pretty high volume > place. It was a great "breaking in" for embedding and microtomy. Luckily > there were also some experienced techs there who saw how much I wanted > to learn, and were willing to help me get better. The "constructive" > criticism stung sometimes, but they did me a huge service. But believe > me, not everyone was helpful or supportive along the way. Try to > ignore those kind of people as much as possible. And I still get > criticized sometimes, make mistakes, and I still have more to learn. > > But here are a couple of other options for you to consider before you > decide to leave, and what I did to get more experience when in your > situation more quickly; > > Take on a second histology job that targets specific skills, tissue, > or techniques you want more experience in. Believe me I have been > criticized and misunderstood for this choice s well many times, but > personally I do not regret any of those experiences now. > > I also feel that small labs are nice to build well rounded skills > since you are usually more of a "jack of all trades" and have less > tendency to do one task over your whole shift from day to day. > Sometimes you just have to identify the environment that is the right fit for you. > > Best of luck to you- and let us know how things turn out! > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: Timothy.Morken@ucsfmedctr.org > > To: optimusprimehistotech@hotmail.com; > > histonet@lists.utsouthwestern.edu > > Date: Tue, 3 Jun 2014 22:51:31 +0000 > > Subject: RE: [Histonet] Should I leave histology world > > CC: > > > > Alpha, it is clear to me, after 30+ years in the field, that some > > are > born with the ability to cut fast AND do well at it. The rest of us > just have to work harder at developing that skill. But it does take > bench time to do it. A recent cache is that it takes 10,000 hours to > become an absolute expert at something - that's about 5 years full > time work. And that's just one skill. > > > > It sounds like you need some good teachers (ie, those who like to > > teach > and like having their students do well). That would be the highest > priority if you want to stay in the field as a bench tech. > > > > If the factory job isn't working out why not look for a smaller lab > > in > which you can get more extensive experience? I really value the fact > that spent my first 11 years in a 4- person lab in which we did > everything from grossing to histo to immunos to EM. It may pay less > initially but may add more value to your lifetime career. > > > > > > Tim Morken > > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies > > UC San Francisco Medical Center > > San Francisco, CA > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alpha > Histotech > > Sent: Tuesday, June 03, 2014 1:35 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Should I leave histology world > > > > Hi everyone, > > > > I wouldn't give too much detail information as the histology world > > is > very small and everyone knows everyone. > > > > I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. > I went to a NAACLS school and have a Associate in Science in > Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs > were graveyard shifts. The first place I worked for was Quest > Diagnostics and I did a good > 3 yrs. The other 2 places I won't mention and I currently still have a > histology job. My problem is all the places I worked were factory > style lab work and they all did derm work. In my career I really only > embedded most of the time. I did occasional other stuff like special > stains both by hand and using Dako Artisan and other things like > cytology cytospin. But I never got to develop in cutting. My first job > in quest..I maybe cutted one time for 2 or 3 weeks before they yanked > me and put me back to embed. My 2nd job put me to cut the last 2 > months (full 8hrs) I was working there. My current job I have been > cutting since April 2014 ( but only 2-3hrs in the day and then I > embed, I have been here now 1 yr, I was embedding most of the time > before th cutting started). I was told by my director I need to speed > up in cutting because corporate is asking why I am not increasing in > speed. And if I don't speed up eventually then they will have to > demote me to a lab aid and give me a pay cut. (where I work and the > state I work in they have lab aids doing alot of stuff without being > certified, it wasn't like that in the other state I am original from > as you have to be state licensed and > ascp) I sometimes laugh inside my head because before my director > hired me I told him I don't have alot experience in cutting. > > > > Now everywhere I have gone...speed is the name of the game. They say > they care about quality but in the end if you can't put up then you > will be put out! So I am just thinking I should just get out of > histology world all together. Every where I have worked unfortunately > have management who believe quantity over quality. OR Do you guys > think I need more time cutting to develop speed? Beforehand I did need > a little learning curve to cut and I have gotten through that now. > It's just the speed that is killing me. And I also see if anyone at my > work detours me for any reason like for example data entry person from > front desk ask for missing gross dictation, then that lost time is > very hard to recover as I am not soooo fast to recover. I feel I may > have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. > One thing I want to say also...until this day I never been written up > for quality issues and I never lost any tissue while embedding. > Embedding I am fast as most histotech (1 block a min or most times > 30-45 secs 1 block) with proper embedding techniques demonstrated > (tissue on same plane, tissue embedded with proper orientation and > follow any other necessary embedding instructions. ) I just feel I > haven't done my time in cutting as I did in embedding to become a fast > cutter. I don't know if its because of working in a derm lab that > management won't wait too long for you to develop like maybe a > hospital lab may do. I was also thinking to find another histo job but > not mention any of my experience so expectation won't be so high and I > can get more time to develop. All of this also causes alot of stress > and anxiety as it gets hard to coop with. What do you guys think and > how I should go about with this. I am also not limited to histology. I > have expertise in 2 other major fields that I wont mention because I > don't want to be identified. I am also in my late 20's. Thanks for > reading my post and I await your opinions as some of you all are veterans in the field of histology. > > > > Thank you > > Alpha Histotech (ASCP HT) > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Wed, 4 Jun 2014 15:46:57 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] Should I leave histology world To: "'Rene J Buesa'" , "Alpha Histotech" , "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF367860D8@ex07.net.ucsf.edu> Content-Type: text/plain; charset=iso-8859-1 Rene is right that everyone needs to find what they are good at. We had a guy who was so-so in cutting speed and always getting flack for not doing enough. And had such a hard time coordinating specials and immunos that he just slowed things down. Then we started doing Mega blocks of whole mount eyes and prostate and it turns out this guy is a savant at cutting large blocks. The pathologists were raving about how good the sections were. He had a job as long as he wanted in that lab just for that! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, June 04, 2014 7:41 AM To: Alpha Histotech; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Should I leave histology world You describe a common situation in histology where the pressure to finish the work on time (the TAT) reigns but that does not necessarily mean that quality has to be sacrificed, although sometimes it does. On the other hand, not all histotechs are "created equal" and some have abilities others don't. Manual dexterity is a most and while some can section at the "normal" rate of 24 blocks/hour, other take twice the time, and it seems that you belong to the later group. For what you wrote it seems that your embedding productivity (about 60 blocks/hour) is the "norm" and, again, others are less or more productive. You also point out that you have worked in 3 places in 6-7 years and that is a quite high turnover never conducent to improve your work and that can be hold "against you" in your Resum? when looking for another position. A histology supervisor has to assure the slides are ready for the pathologists on a timely basis so one of the things that have to be done is to identify amongst the staff? "who does what best" meaning who can deliver quality in a timely basis. I think that you, already in your late 20's,?should start trying to answer several questions: 1- why did you in the first place?decided to become a histotech and if that was a "wise" selection? 2- if you are experiencing the same problems in the 3 places where you worked, it seems clear to me that the issue is with you and not with the trade. It will be the same at least in these types of high volume labs where higher productivity are required. If you really like histology and think that you need more training to achieve?sectioning speed, you have to switch to another type of lab. Try to solicit work in a research or university lab where you will have enough time to train properly and where finishing the work "yesterday"?is no usually a concern. Also think that not all histotechs have the same ability or speed. I had supervised along my career as supervisor scores of histotechs and some just cannot?section fast, it is not?within their abilities but can excel in some other tasks. My duty was to detect the task that they could complete best and, without totally frustrating their lives, assign them to those tasks. Also you could try to improve your speed on your own time, and demonstrate that you are also willing to try to improve. So I think that it is time for you to do one of the following: 1- change career 2- change type of lab 3- adapt?and adquire sectioning speed or 4- find amongst the many tasks?that histology provide, the one?where you can excel and at the same find satisfaction. What you cannot do is to keep doing the same and expect to find satisfaction or obtain a different result given your ability. Think hard and honestly what you want to do for the rest of your life that most likely will be long. Ren? J.? On Tuesday, June 3, 2014 4:35 PM, Alpha Histotech wrote: Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out!? So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with.? What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified.? I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 4 Jun 2014 10:06:01 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: fluorescent scope and slide scanner To: "Santiago, Albert" , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05C9A@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Albert It all depends on your through put and how many slides you need to scan daily. There are so many other things you need to consider prior to purchasing a scanner. If you go to the DPA website you can get a lot on information on digital pathology www.digitalpathologyassociation.org The larger vendors such as Leica/Aperio, Olympus, Philips, GE, Ventana will have different solutions and packages for what you may need to include storage and database, reporting, image analysis, etc. You need to think about how you want to use the scanner prior to purchasing one. I have contact information for most of the vendors so if you want me to provide that to you I can. Now I'm going to give a shameless plug - Jesus Elin, Bill DeSalvo and myself will be giving an all day workshop on this very topic at NSH in Austin it's on Saturday. If you can't make it to that meeting then the DPA has Pathology Visions in October you would be able to spend time with a lot of the vendors that are in this digital pathology space and in addition to that Bill DeSalvo and I will also presenting on this topic at the Region VII meeting in Phoenix in July. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santiago, Albert Sent: Wednesday, June 04, 2014 6:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fluorescent scope and slide scanner Hello my colleagues in histoland, We are in the market for a Slide Scanner and a Fluorescent Microscope, if anyone has any information on any of these products please share with me. Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago@uphs.upenn.edu The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 4 Jun 2014 11:20:32 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] HER2 by IHC- Fixation To: "histonet@lists.utsouthwestern.edu" Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82214D@MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters, I hope you guys are doing great. Please I wanted to confirm whether it is true that the CAP has changed the HER2 Fixation time from 6 - 48 hours to 6 - 72 hours. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 11 Date: Wed, 4 Jun 2014 16:22:52 +0000 From: "Weems, Joyce K." Subject: RE: [Histonet] HER2 by IHC- Fixation To: "'Adesupo, Adesuyi (Banjo)'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" That is true. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Wednesday, June 04, 2014 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 by IHC- Fixation Hi Histonetters, I hope you guys are doing great. Please I wanted to confirm whether it is true that the CAP has changed the HER2 Fixation time from 6 - 48 hours to 6 - 72 hours. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 12 Date: Wed, 4 Jun 2014 16:50:05 +0000 (UTC) From: koellingr@comcast.net Subject: [Histonet] leaving histology question research is still an option To: optimusprimehistotech@hotmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <1831821306.3228072.1401900605181.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 Alpha Histotech, ?? wanted to be sure that I did??NOT tell you to drop everything in life to look to research exclusively.?? So I cut and pasted this from my original message "Research histology should not be overlooked I stand by that statement. ?? I agree with Emily that funding in research is (stupidly for this nation) difficult.?? But it is not zero.?? PhD's do NOT saturate histotech jobs in research labs.?? There might be a few, maybe some, maybe a lot in some places but not all.?? No one, even a clinical lab, will guarantee that job for years and years.?? I know many histotech/non-histotech "techs" who have been through 5-6 different more molecular labs in the same building for??over 30 years.?? So have 30 years seniority in the system.?? Grant runs out and you move to a different lab (and is way easier having had made connections in first lab).?? I made 3x in research industry then I could ever have made in clinical histo lab.?? Is not for everyone but also not something to dismiss as hopeless.?? If you ever do research, you will find that "N of 1" is not reliable to base everything and every decision on.?? Best of luck.?? Search for those options several others have given but don't dismiss my suggestion outright. ?? Ray in Seattle (histotech from 1960's who could only retire now because of Research histology) ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 5 **************************************** From tbraud <@t> holyredeemer.com Wed Jun 4 14:57:58 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Jun 4 14:59:35 2014 Subject: [Histonet] RE: Acid Clean Glassware In-Reply-To: <20140604190246.71C7B1E808B@trendmess-svr.holyredeemer.local> References: <20140604190246.71C7B1E808B@trendmess-svr.holyredeemer.local> Message-ID: Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. Acid Cleaned glassware (Abbott, Tanya) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From bakevictoria <@t> gmail.com Wed Jun 4 15:06:03 2014 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Jun 4 15:06:06 2014 Subject: [Histonet] Alpha Histotech - leaving the world of histology Message-ID: Hi Alpha, I've been From SSCALISE <@t> beaumont.edu Wed Jun 4 17:48:38 2014 From: SSCALISE <@t> beaumont.edu (Sharon Scalise) Date: Wed Jun 4 17:48:44 2014 Subject: [Histonet] Validating slides Message-ID: <190D98228ADC1747BCE27019B78FD8F3011F7B0C@EXMAIL06.ms.beaumont.edu> I am looking for comments about validating slides in histology/immuno. We have always validated our charged slides if we went to a new brand/vendor in order to ensure that the tissue sections will adhere properly during immuno procedures and certain special stains. Once we found them to be acceptable, we continue to use them, lot to lot, and never validate as long as we do not change brand/vendor. We recently ran into a problem with slides that we received by accident (similar packaging, different slide) and they were used for some immuno cases. The sections on many of the slides fell off and the stains had to be repeated. We realized the problem and pulled the slides out of service. We contacted the vendor and they said that those slides should have been pulled from the shelf, that it was a bad batch and they should have never been sent to anyone, yet alone incorrectly sent to us. I started to think that we might want to consider validating new lots we receive to ensure that each lot is acceptable. As we began this discussion there were people who felt that in any lot you could have slides that are acceptable and maybe some boxes that give you problems. How do you know if all slides in one lot are treated exactly the same? Can you really use lot number to verify quality when it comes to slides? I am not exactly sure of the process used to "charge" the slides so I don't feel qualified to answer these questions. What is everyone else doing about slide validation? Is it really necessary? (I can say that in 30 years I have never seen it done with each new lot, but I have been at the same job for the past 18 years). Thanks for your input! Sharon Scalise, HTL(ASCP) Histology Supervisor-Anatomic Pathology Beaumont Health System 3601 W. 13 Mile Rd. Royal Oak, MI 48073 248 898-5981 sscalise@beaumont.edu From tony.henwood <@t> health.nsw.gov.au Wed Jun 4 18:20:00 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jun 4 18:20:21 2014 Subject: [Histonet] RE: Acid Clean Glassware In-Reply-To: References: <20140604190246.71C7B1E808B@trendmess-svr.holyredeemer.local> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00EB0EC@xmdb04.nch.kids> No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience is that they are GMS (using chromic acid) negative. It is possible that our pathologists aren't aware that the "GMS" that their lab does might be using Periodic acid instead of Chromic acid. Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of chromic acid. The literature often does not report the exact GMS used which makes clear understanding of the histochemical results difficult. Pneumocystis will not be easy to see unless chromic acid is used (the mucin stains strongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 5 June 2014 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acid Clean Glassware Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. Acid Cleaned glassware (Abbott, Tanya) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Wed Jun 4 18:24:31 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Jun 4 18:24:50 2014 Subject: [Histonet] GMS better than PAS for Fungi Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00EB10B@xmdb04.nch.kids> Thought that the subject title should be changed. (References available on request) No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience is that they are GMS (using chromic acid) negative. It is possible that our pathologists aren't aware that the "GMS" that their lab does might be using Periodic acid instead of Chromic acid. Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of chromic acid. The literature often does not report the exact GMS used which makes clear understanding of the histochemical results difficult. Pneumocystis will not be easy to see unless chromic acid is used (the mucin stains strongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 5 June 2014 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acid Clean Glassware Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. Acid Cleaned glassware (Abbott, Tanya) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Denice.Stiner <@t> nahealth.com Thu Jun 5 04:04:12 2014 From: Denice.Stiner <@t> nahealth.com (Denice Stiner) Date: Thu Jun 5 04:05:13 2014 Subject: [Histonet] RE: slide drying/curing before filing In-Reply-To: References: , Message-ID: Everywhere I've worked uses quick drying mounting media and files within the next day or so. Glass coverslips as well. Denice M. Stiner, HT (ASCP) Histology Supervisor Flagstaff Medical Center Northern Arizona Health Care denice.stiner@nahealth.com 928-773-2489 Pathology 928-773-2283 Fax ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. [Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, June 04, 2014 11:35 AM To: 'Lake, Kim S'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slide drying/curing before filing We use quick drying Richard Allen Mounting Media - 22 050 102, purchased through Fisher Healthcare. We file the next day!! Glass coverslips... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S Sent: Wednesday, June 04, 2014 1:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide drying/curing before filing According to the Histonet archives, this hasn't been discussed for almost a decade. How are labs drying/curing their slides before they are filed? We are a small oral pathology laboratory and we hand-coverslip using Richard Allen mounting medium and glass coverslips. After cases are signed out slides are placed in their cardboard folders in a 37C oven for at least a week, then are filed in metal slides drawers. This works perfectly well, but we would like to simplify this, if possible. A search of the archive showed a great deal of variation in slide drying protocol, I'm interested in hearing what everyone else is up to. Of course, I'm hoping to get lots of people saying that they file all of last week's room temp slides on Monday morning and it works like a charm! Thanks! Kim Lake, MLS (ASCP)CM Laboratory Manager Oral Pathology Laboratory University of Iowa College of Dentistry Phone (319) 384 4433 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Thu Jun 5 08:21:20 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Thu Jun 5 08:21:26 2014 Subject: [Histonet] Validating slides Message-ID: My experience with slide quality is that no matter who or where you get your slides from you can have variability even within the same pack. We found the slide we felt to be the most consistent high quality and we use that never checking lot to lot validity. An immuno technical specialist showed us how you can quickly check the quality of the charge on slides (you may already know this). He took a slide and dipped it into hematoxylin and then laid it on the counter. You can actually see the heme being repelled by the poor quality slide, whereas the heme on the higher quality slide uniformly coated the entire slide. We took 3 or 4 slides from the pack (from front, middle, and back) to test them for consistency. Try it, its amazing that you can see the poor slide repelling the heme and that's what it does to your immuno reagents. You may use this when determining which slides work best for your lab. Hope this helps a little. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/4/14 4:48 PM, "Sharon Scalise" wrote: >I am looking for comments about validating slides in histology/immuno. >We have always validated our charged slides if we went to a new >brand/vendor in order to ensure that the tissue sections will adhere >properly during immuno procedures and certain special stains. Once we >found them to be acceptable, we continue to use them, lot to lot, and >never validate as long as we do not change brand/vendor. We recently ran >into a problem with slides that we received by accident (similar >packaging, different slide) and they were used for some immuno cases. >The sections on many of the slides fell off and the stains had to be >repeated. We realized the problem and pulled the slides out of service. >We contacted the vendor and they said that those slides should have been >pulled from the shelf, that it was a bad batch and they should have never >been sent to anyone, yet alone incorrectly sent to us. >I started to think that we might want to consider validating new lots we >receive to ensure that each lot is acceptable. As we began this >discussion there were people who felt that in any lot you could have >slides that are acceptable and maybe some boxes that give you problems. >How do you know if all slides in one lot are treated exactly the same? >Can you really use lot number to verify quality when it comes to slides? >I am not exactly sure of the process used to "charge" the slides so I >don't feel qualified to answer these questions. >What is everyone else doing about slide validation? Is it really >necessary? (I can say that in 30 years I have never seen it done with >each new lot, but I have been at the same job for the past 18 years). > >Thanks for your input! > >Sharon Scalise, HTL(ASCP) >Histology Supervisor-Anatomic Pathology >Beaumont Health System >3601 W. 13 Mile Rd. >Royal Oak, MI 48073 >248 898-5981 >sscalise@beaumont.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Thu Jun 5 08:21:59 2014 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Thu Jun 5 08:22:10 2014 Subject: [Histonet] RE: Should I leave histology world Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881862D11F@D1PWPEXMBX05.mdanderson.edu> I agree with Tim. Take a part-time job doing what you need to work on if possible. When my embedding skills needed to be enhanced, I took a job embedding early mornings. One time I took a job cutting for about 2 years, and that helped my speed. I still am not the best cutter, but my speed increased and my errors decreased. In your current lab, ask for help from other techs as to setting goals and meeting deadlines. Make it a game, not to be taking risks and possibly injuring yourself, but a little friendly competition could help. My students compete with each other to see who can finish fastest, with the least amount of errors, and the winner doesn't do the dishes that next day. Remember, this is your livelihood, so you need to find out how to increase your speed and not sacrifice quality. You should set up a time daily to develop your technique and work on it. Even if you have to switch duties to get this done, your manager should support you to keep you. Find out what is slowing you down, is your station not set-up properly, do you not have all the tools needed, are the blocks embedded correctly, is the angle correct? This can slow you down without you realizing it. Create your own technique and make it great. I never could line up sections side-by-side on slide for biopsies, so I just began to cut my ribbons longer and picked sections up in pairs. It worked for me. Anything helps!! Good luck. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 ------------------------------ Message: 12 Date: Tue, 3 Jun 2014 16:34:51 -0400 From: Alpha Histotech Subject: [Histonet] Should I leave histology world To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) ------------------------------ Message: 13 Date: Tue, 3 Jun 2014 21:00:02 +0000 From: "Nails, Felton" Subject: RE: [Histonet] Should I leave histology world To: Alpha Histotech , "histonet@lists.utsouthwestern.edu" Message-ID: <327E034F1892504289B7A17EC71DF9F30B5FDF@TCFMSG03.ad.texaschildrenshospital.org> Content-Type: text/plain; charset="us-ascii" I always tell my students if you can cut, you can get a job. It appears that you school did not properly prepare you for the demands of an average histology job. You need to take every opportunity to work on your craft and the major focus of histology is cutting. With 6 to 7 years of experience you are expected to know the basics and cutting is basic. Date: Tue, 3 Jun 2014 21:28:50 +0000 (UTC) From: nmhisto@comcast.net Subject: Re: [Histonet] Should I leave histology world To: Felton Nails Cc: HISTONET Message-ID: <599903481.2425987.1401830930956.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 Felton, I disagree!?? The training this tech underwent must obviously have??covered basic histology but you cannot guarantee??that a trained student will find a laboratory that will give him/her the opportunity to develop speed while not sacrificing quality. Having the training does not warrant that the new tech will be able to cut a certain number of blocks per hour to satisfy the demands of an employer's??spreadsheet mentality.?? A good laboratory manager will make it possible for that "newbie" to have the time at the microtome (or the embedding station, etc.) to develop the speed that comes with an experienced eye.?? Providing that newly-minted tech the time necessary just makes sense to the employer and the tech.????Realizing full well (after 40+ years as an HT) that this is not a perfect world and that other factors weigh heavily, encouragement pays off in quality, quantity and loyalty.?? Perhaps what Alpha Histotech needs to do (if the lab manager is an open-minded, logical individual) is to discuss this issue with that manager and allow the manager to understand that this new tech has the enthusiasm but needs "time in grade".?? This is too critical an issue to begin to lose techs (as few as there are coming into the field) by an employer's requirement to produce quantity without the absolute necessity of quality. Regards to all! ------------------------------ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 3 **************************************** From mjones <@t> metropath.com Thu Jun 5 08:23:24 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Thu Jun 5 08:23:31 2014 Subject: [Histonet] Validating slides Message-ID: P.S. We use Platinum series from Mercedes Medical (so far we're okay) previously we used Fisher Superfrost +, good quality, however these were very expensive. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/4/14 4:48 PM, "Sharon Scalise" wrote: >I am looking for comments about validating slides in histology/immuno. >We have always validated our charged slides if we went to a new >brand/vendor in order to ensure that the tissue sections will adhere >properly during immuno procedures and certain special stains. Once we >found them to be acceptable, we continue to use them, lot to lot, and >never validate as long as we do not change brand/vendor. We recently ran >into a problem with slides that we received by accident (similar >packaging, different slide) and they were used for some immuno cases. >The sections on many of the slides fell off and the stains had to be >repeated. We realized the problem and pulled the slides out of service. >We contacted the vendor and they said that those slides should have been >pulled from the shelf, that it was a bad batch and they should have never >been sent to anyone, yet alone incorrectly sent to us. >I started to think that we might want to consider validating new lots we >receive to ensure that each lot is acceptable. As we began this >discussion there were people who felt that in any lot you could have >slides that are acceptable and maybe some boxes that give you problems. >How do you know if all slides in one lot are treated exactly the same? >Can you really use lot number to verify quality when it comes to slides? >I am not exactly sure of the process used to "charge" the slides so I >don't feel qualified to answer these questions. >What is everyone else doing about slide validation? Is it really >necessary? (I can say that in 30 years I have never seen it done with >each new lot, but I have been at the same job for the past 18 years). > >Thanks for your input! > >Sharon Scalise, HTL(ASCP) >Histology Supervisor-Anatomic Pathology >Beaumont Health System >3601 W. 13 Mile Rd. >Royal Oak, MI 48073 >248 898-5981 >sscalise@beaumont.edu > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Jun 5 09:20:12 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jun 5 09:20:27 2014 Subject: [Histonet] RELIA Histology Careers Bulletin 6-5-2014 Summer is Here!! Message-ID: <00b301cf80c9$43947ba0$cabd72e0$@earthlink.net> Hi Histonetters!! What tells you that Summer is here? That first bite of watermelon OR sip of ice cold lemonade on a hot day? That first dip in the shimmering turquoise waters of a pool, lake or ocean? That Cookout, Baseball Game, State Fair, Fresh Corn? PROM, GRADUATION. SUMMER VACATION!! I Love it all, except maybe the heat and bathing suit shopping (UGH!!) Another way I can tell that Summer is here is that my phone also starts ringing off the hook. Clients are calling from all over the country. It happens every year right after Memorial Day. I Have A Bunch Of New Opportunities To Tell You About And New Ones Coming In EVERY DAY!! So please take a look at my current openings. If something looks interesting let me know. If you know someone who might be interested please pass along the information. Remember if I place someone you refer to me you will earn a referral fee. If the opportunity for you is someplace else drop me an email to remind me where you want to be and what you want to do. I want YOU to be the person that pops in MY head when that client calls!!! Current Open Histology Opportunities: AP and Cytology Manager - Charlotte, NC IHC/QC Manager - San Francisco, CA IHC Supervisor - Dallas, TX Lead Histology Tech - East of St. Louis in Illinois Lead Histology Tech - Zanesville, OH Grossing Histology Tech - Roswell, NM Grossing Histology Tech - Las Cruces, NM Histotechnician - Nights - Nashville, TN Junior Histotech - Days - Boston, MA Electron Microscopy Tech - Uniondale, NY All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent & some you will only see here because they are RELIA Exclusives!!! If you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus. I will be waiting for your call at 407-353-5070 all day Friday June 6th so call or email me at relia1@earthlink.net with a time and the best number to call you!!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 From tbraud <@t> holyredeemer.com Thu Jun 5 08:29:29 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jun 5 10:41:19 2014 Subject: [Histonet] RE: Churukian Silver stain for Fungus In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157E00EB0EC@xmdb04.nch.kids> References: <20140604190246.71C7B1E808B@trendmess-svr.holyredeemer.local> <6D6BD1DE8A5571489398B392A38A7157E00EB0EC@xmdb04.nch.kids> Message-ID: I beg to differ. I think you are confusing stains. I am not talking about using a Periodic Acid Schiff's stain (PAS) to stain fungus. I am referring to the Churukian Microwave Ammoniacal Silver stain for fungus. It oxidizes with Periodic Acid and the Silver solution is similar to what is used for most Reticulum stains. It stains the exact same organisms as a Grocott's Methenamine Silver (GMS), just without staining the elastic fibers. Our pneumocystis stains using the Churukian Ammoniacal Silver stain are just beautiful. This method has been taught at NSH workshops and has been widely used in published literature. As soon as I can figure out how to post pictures, I will send some pictures of a a pneumocystis control and an aspergillus control stained with the Churukian method. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Wednesday, June 04, 2014 7:20 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: Acid Clean Glassware No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience is that they are GMS (using chromic acid) negative. It is possible that our pathologists aren't aware that the "GMS" that their lab does might be using Periodic acid instead of Chromic acid. Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of chromic acid. The literature often does not report the exact GMS used which makes clear understanding of the histochemical results difficult. Pneumocystis will not be easy to see unless chromic acid is used (the mucin stains strongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 5 June 2014 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acid Clean Glassware Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. Acid Cleaned glassware (Abbott, Tanya) ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ********* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ ********* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From christina.kreutzer01 <@t> gmail.com Thu Jun 5 11:04:18 2014 From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer) Date: Thu Jun 5 11:04:20 2014 Subject: [Histonet] PBS and PB Message-ID: Hu histoneters, we are currently standardizing our buffer protocols/recipes and I am wondering what recipes you are using for PBS and salt free PB. Would appreciate any kind of help for optimizing my database. All the best Christina From Nancy_Schmitt <@t> pa-ucl.com Thu Jun 5 12:41:13 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Jun 5 12:41:23 2014 Subject: [Histonet] GATA-3 Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36D9CADFD0@PEITHA.wad.pa-ucl.com> Hi All- Looking for recommendations on the use of the antibody GATA-3 on the Leica Bond Max. Are you satisfied with the results? Thank you in advance- Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 visit us at www.uclaccess.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From sheryl.stephenson <@t> ag.state.nj.us Thu Jun 5 13:12:01 2014 From: sheryl.stephenson <@t> ag.state.nj.us (Stephenson, Sheryl) Date: Thu Jun 5 13:12:04 2014 Subject: [Histonet] Rabies tissue Fixed in Formalin. Message-ID: Hi, Does anyone have any knowledge and literature on whether or not 10%NBF inactivates rabies in animal tissue during fixation? Thanks, Sheryl. From rsrichmond <@t> gmail.com Thu Jun 5 13:13:43 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Jun 5 13:13:46 2014 Subject: [Histonet] Re: GMS better than PAS for fungi Message-ID: Freida Carson wrote what was probably the definitive study of the subject about 15 years ago. What stain you need depends on what fungus you're trying to stain. PAS does fine for Candida. The most critical test of a fungal stain is its ability to stain old ("obsolete") Histoplasma. Here's the abstract of her article: Inconsistent Detection of Histoplasma capsulatum with Periodic Acid Oxidation in the Grocott Methenamine-Silver Nitrate (GMS) Fungus Stain Freida L. Carson, Jerry Fredenburgh, and John E. Maxwell 1. Department of Pathology, Baylor University Medical Center 2. Richard-Allan Scientific Kalamazoo MI 3. Glenwood Regional Medical Center, West Monroe LA J Histotechnol [June] 1999;22:119 Abstract Grocott's modification of the Gomori methenamine silver staining technique is widely recognized as the best method for the detection of fungal elements in tissues. Although chromic acid remains the preferred oxidizing agent in many laboratories, in recent years there has been a trend toward replacing it with periodic acid because of safety, stability, and environmental issues. Our experience indicated that 1% periodic acid oxidation for 5 to 30 min at room temperature is insufficient for consistent detection of H. capsulatum, although adequate staining of Aspergillus sp. is obtained. Consequently, false-negative results may be reported. We recommend that the period of oxidation with periodic acid should be extended to 1 hr at 56 to 60?C to ensure consistent demonstration of H. capsulatum. These observations also underscore the importance of the use of matched positive controls when analyzing fungal stains. (The J Histotechnol 22:119-122, 1999) Bob Richmond Samurai Pathologist Maryville TN From lori.garcia <@t> medtronic.com Thu Jun 5 13:20:07 2014 From: lori.garcia <@t> medtronic.com (Garcia, Lori, M.Sc.) Date: Thu Jun 5 13:20:26 2014 Subject: [Histonet] RE: Rabies tissue Fixed in Formalin. In-Reply-To: References: Message-ID: <8214022B32A9DE4985A37EA7AE814DA726CFF9@MSPM1BMSGM41.ent.core.medtronic.com> Hi Sheryl, Yes, 24 hour fixation will do it. The only thing that formalin does not inactivate is prions. Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Thursday, June 05, 2014 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rabies tissue Fixed in Formalin. Hi, Does anyone have any knowledge and literature on whether or not 10%NBF inactivates rabies in animal tissue during fixation? Thanks, Sheryl. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From shive003 <@t> umn.edu Thu Jun 5 13:29:58 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jun 5 13:30:06 2014 Subject: [Histonet] Rabies tissue Fixed in Formalin. In-Reply-To: References: Message-ID: *SUSCEPTIBILITY TO DISINFECTANTS*: Rabies virus is inactivated by exposure to 70% ethanol, phenol, formalin, ether, trypsin, ?-propiolactone, and some other detergents(3 ). (Found in the Public Health Agency of Canada article http://www.phac-aspc.gc.ca/lab-bio/res/psds-ftss/rab-eng.php) We often do IHC testing for Rabies on formalin-fixed, paraffin-embedded tissues here, with no extra precautions needed. Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu On Thu, Jun 5, 2014 at 1:12 PM, Stephenson, Sheryl < sheryl.stephenson@ag.state.nj.us> wrote: > Hi, > Does anyone have any knowledge and literature on whether or not 10%NBF > inactivates rabies in animal tissue during fixation? > Thanks, > Sheryl. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TGoins <@t> mt.gov Thu Jun 5 14:24:40 2014 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Jun 5 14:24:50 2014 Subject: [Histonet] RE: Rabies tissue Fixed in Formalin. In-Reply-To: References: Message-ID: Sheryl - NBF will inactivate the rabies virus but precautions should be taken. If a rabies case is suspected but infection can not be confirmed or ruled out due to the tissue being unsuitable for rabies testing by fresh tissue fluorescent antibody assay, the tissue is cut in for FFPE sections ONLY by personnel that are vaccinated against rabies. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Thursday, June 05, 2014 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rabies tissue Fixed in Formalin. Hi, Does anyone have any knowledge and literature on whether or not 10%NBF inactivates rabies in animal tissue during fixation? Thanks, Sheryl. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JLATREMO <@t> gru.edu Thu Jun 5 14:28:28 2014 From: JLATREMO <@t> gru.edu (Latremouille, John) Date: Thu Jun 5 14:28:40 2014 Subject: [Histonet] Request for Non-fluorescent Sealant Message-ID: <1401996522317.56374@gru.edu> Hello, I am wondering if anyone would know of a sealant that is non-fluorescent (or doesn't leak). I am using a biorad duo chamber slide which has two slots for each chamber, I need to measure the fluorescence of some sample but Rubber Cement and the nail polish I have tried have both had some interfering fluorescence. Any help is appreciated! -John From algranth <@t> email.arizona.edu Thu Jun 5 14:48:35 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Jun 5 14:48:52 2014 Subject: [Histonet] C-14 radioactive tissues Message-ID: Does anybody work with tissue with carbon 14? if so, can you share a protocol? Our radiation control people are more interested that I know atomic structure and how instruments are used to measure radioactivity work than how to actually work with the tissue. Are lab coats/aprons sufficient and nitrile gloves as protection? I understand you can breathe the stuff - face shield? Thanks! Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From shive003 <@t> umn.edu Thu Jun 5 14:50:22 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jun 5 14:50:30 2014 Subject: [Histonet] RE: Rabies tissue Fixed in Formalin. In-Reply-To: References: Message-ID: Yes, Tresa mentions an important caution. Personnel need to be vaccinated against rabies if they're cutting in tissue. Jan Shivers On Thu, Jun 5, 2014 at 2:24 PM, Goins, Tresa wrote: > Sheryl - > > NBF will inactivate the rabies virus but precautions should be taken. > If a rabies case is suspected but infection can not be confirmed or ruled > out due to the tissue being unsuitable for rabies testing by fresh tissue > fluorescent antibody assay, the tissue is cut in for FFPE sections ONLY by > personnel that are vaccinated against rabies. > > Tresa > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl > Sent: Thursday, June 05, 2014 12:12 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Rabies tissue Fixed in Formalin. > > Hi, > Does anyone have any knowledge and literature on whether or not 10%NBF > inactivates rabies in animal tissue during fixation? > Thanks, > Sheryl. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From amosbrooks <@t> gmail.com Thu Jun 5 15:51:46 2014 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Jun 5 15:51:54 2014 Subject: [Histonet] Re: Histonet Digest, Vol 127, Issue 3 In-Reply-To: <538e4027.66493c0a.2702.219dSMTPIN_ADDED_MISSING@mx.google.com> References: <538e4027.66493c0a.2702.219dSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi Alpha ;-), I usually tell new techs that you will not find and should not look for the perfect job right out of school. It is imperative that you get yourself a broad base of experience before settling down with something you really like. Eventually you will find your niche. You have started off with one type of lab, the factory. This is not for everyone, but I have known folks that do perfectly well there and wouldn't want anything else. After working there for a while and learning the speed and basic demands of such a facility you should try to locate a position in a small hospital. The pay is not usually as good there (so this may be a better place to start), but you will be expected to know a little of everything. The volume isn't as high so you are usually not tied to one task all day at warp speed. You are closer to the patients, often passing them in the hallways. There are faces to associate with the work you do. (Not directly associated of course, but I hope you get my drift.) There are different stresses and rewards here, and again it isn't for everyone, but for some it is perfect. Try research labs and pharmaceutical labs as well. You may find yourself working with animals. Certainly not for everyone, but you learn a lot. Perhaps an EM or hard tissue lab might be worth a try. Regardless of where you end up settling down and staying, the different environments will teach you the best and worst of each one. You will become a far better tech with a broad array of experience. I spent time in a tiny hospital lab, a large reference lab, a large university lab and ended up in a research lab. I wouldn't trade one of the labs I was in regardless of how well or poorly I fit into them since they all taught me something I can use now. Also remember *nothing* is perfect. There will always be things that will drive you nuts. Primadonna pathologists, perfectionist managers or insane coworkers. But you will also find pathologists that you can learn so much from and managers that will really help you build your career and really good friends to work with. You sometimes have to take the bad with the good or at least hope for more of the latter than the former. Keep trying, Amos PS: You are very right to try to not sling stones and keep a modicum of anonymity about this subject. There are stories about those that don't have that foresight, and that is proof of the point. It is indeed a small world. On Tue, Jun 3, 2014 at 5:37 PM, wrote: > Message: 12 > Date: Tue, 3 Jun 2014 16:34:51 -0400 > From: Alpha Histotech > Subject: [Histonet] Should I leave histology world > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi everyone, > > I wouldn't give too much detail information as the histology world is very > small and everyone knows everyone. > > I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I > went to a NAACLS school and have a Associate in Science in Histology. In > the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard > shifts. The first place I worked for was Quest Diagnostics and I did a good > 3 yrs. The other 2 places I won't mention and I currently still have a > histology job. > <<< SNIP >>> > I am also not limited to histology. I have expertise in 2 other major > fields that I wont mention because I don't want to be identified. I am > also in my late 20's. Thanks for reading my post and I await your opinions > as some of you all are veterans in the field of histology. > > Thank you > Alpha Histotech (ASCP HT) > From Richard.Cartun <@t> hhchealth.org Thu Jun 5 15:54:15 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Jun 5 15:54:21 2014 Subject: [Histonet] RE: GATA-3 In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36D9CADFD0@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36D9CADFD0@PEITHA.wad.pa-ucl.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E1C22F103@HHCEXCHMB03.hhcsystem.org> We use Cell Marque's mAb (clone L50-823) at a dilution of 1:500 (15' primary antibody incubation) following H1 (low-pH) retrieval. Very satisfied. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, June 05, 2014 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GATA-3 Hi All- Looking for recommendations on the use of the antibody GATA-3 on the Leica Bond Max. Are you satisfied with the results? Thank you in advance- Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 visit us at www.uclaccess.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tony.henwood <@t> health.nsw.gov.au Thu Jun 5 20:16:50 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jun 5 20:17:09 2014 Subject: [Histonet] RE: Churukian Silver stain for Fungus In-Reply-To: References: <20140604190246.71C7B1E808B@trendmess-svr.holyredeemer.local> <6D6BD1DE8A5571489398B392A38A7157E00EB0EC@xmdb04.nch.kids> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00EB5D6@xmdb04.nch.kids> Terri, I was referring to the incorrect use of periodic acid in the GMS stain. The Grocott's Methenamine silver stain uses chromic acid, not Periodic acid. There are many instances in the literature where using a classic GMS has been found superior for demonstrating fungus rather than PAS. I am concerned that Periodic acid - silver staining has not been validated using cases where classic GMS has been shown to be more sensitive than PAS (see below) or in cases of pseudofungi, which are PAS positive but overall GMS negative. An appreciation of the difficulties in relying on Periodic acid rather than chromic acid for GMS staining can be seen in these articles: Chandler, F. W., & Watts, J. C. (1995). Fungal diseases. Journal of Histotechnology, 18(3), 247-252 Carson, F. L., Fredenburgh, J., & Maxwell, J. E. (1999). Inconsistent detection of histoplasma capsulatum with periodic acid oxidation in the Grocott methenamine-silver nitrate (GMS) fungus stain. Journal of histotechnology, 22(2), 119-122. Carson, F. L., & Richmond, R. S. (2010). Periodic acid cannot replace chromic acid in Grocott's method for fungi. Biotechnic & histochemistry: official publication of the Biological Stain Commission, 85(4), 270. Henwood, A. F., Prasad, L., & Bourke, V. M. (2013). The application of heated detergent dewaxing and rehydration to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing. Journal of Histotechnology, 36(2), 45-50. Song, D. E., Kahn, A. G., Khang, S. K., & Ro, J. Y. (2005). Pseudofungi in pericolic lymph nodes. Archives of pathology & laboratory medicine, 129(4), e97-e100. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Terri Braud [mailto:tbraud@holyredeemer.com] Sent: Thursday, 5 June 2014 11:29 PM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: RE: Churukian Silver stain for Fungus I beg to differ. I think you are confusing stains. I am not talking about using a Periodic Acid Schiff's stain (PAS) to stain fungus. I am referring to the Churukian Microwave Ammoniacal Silver stain for fungus. It oxidizes with Periodic Acid and the Silver solution is similar to what is used for most Reticulum stains. It stains the exact same organisms as a Grocott's Methenamine Silver (GMS), just without staining the elastic fibers. Our pneumocystis stains using the Churukian Ammoniacal Silver stain are just beautiful. This method has been taught at NSH workshops and has been widely used in published literature. As soon as I can figure out how to post pictures, I will send some pictures of a a pneumocystis control and an aspergillus control stained with the Churukian method. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Wednesday, June 04, 2014 7:20 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: Acid Clean Glassware No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience is that they are GMS (using chromic acid) negative. It is possible that our pathologists aren't aware that the "GMS" that their lab does might be using Periodic acid instead of Chromic acid. Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of chromic acid. The literature often does not report the exact GMS used which makes clear understanding of the histochemical results difficult. Pneumocystis will not be easy to see unless chromic acid is used (the mucin stains strongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 5 June 2014 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acid Clean Glassware Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. Acid Cleaned glassware (Abbott, Tanya) ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ********* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ ********* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From mw <@t> personifysearch.com Fri Jun 6 07:15:58 2014 From: mw <@t> personifysearch.com (Matt Ward) Date: Fri Jun 6 07:16:05 2014 Subject: [Histonet] New Position Alert! IHC Field Specialist Message-ID: <135601cf8181$13e4f4d0$3baede70$@personifysearch.com> Good Morning Histonet! We have had a new opportunity open with a world leading manufacturer of histology products. Our client is growing and searching for a histology professional to join their team on the North East ideally based in Northern New Jersey or New York City. This role will work in the field with sales reps as the technical specialist. This opportunity is perfect for someone who is looking to break out of the laboratory and into the field. The opportunity offers a competitive base salary + bonus + great benefits. If this sounds like something you would be interested in learning more about, please contact me at mw@personifysearch.com , or call 800.875.6188 ext. 103. I look forward to connecting soon! Best Regards, Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com From tbraud <@t> holyredeemer.com Fri Jun 6 07:59:30 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Jun 6 07:59:35 2014 Subject: [Histonet] RE: Churukian Silver stain for pneumocystis and fungus In-Reply-To: <20140605161638.7B2151E80FC@trendmess-svr.holyredeemer.local> References: <20140605161638.7B2151E80FC@trendmess-svr.holyredeemer.local> Message-ID: For those who have written off list for the Churukian stain procedure, I've included it here. It is also sold in the USA in kit form by Polyscientific (no plug here, just stating) PRINCIPLE: Fungi and Pneumocystis organisms are oxidized using periodic acid and then impregnated with ammoniacal silver solution. The silver is then toned from brown to black with gold chloride. Any unreduced silver is removed with sodium thiosulfate, leaving organisms stained black against a green counterstained background. SPECIMENS: Formalin fixed, paraffin embedded tissue cut at 4-5 microns or 95% alcohol fixed smears or monolayer cell preparations. CONTROL: Any tissue known to contain a fungus or Pneumocystis organisms REAGENTS: 1. Periodic acid 1% Aqueous 2. Ammoniacal GMS solution (Silver) 3. Gold Chloride 0.5% Aqueous 4. Sodium Thiosulfate 5% Aqueous 5. Fast Green Working Solution All working solutions are purchased from PolyScientific Kit #K101. Individual components may be purchased separately as they are exhausted. PROCEDURE: 1. Deparaffinize and hydrate to water 2. Using a plastic Coplin jar with the lid resting upside down on top, microwave 50.ml of 1% Periodic Acid at for 30 seconds at power 5. 3. Place slides in the hot solution, covered, for 5 minutes. 4. Wash in 5 changes of DI Water. 5. Using a plastic Coplin jar with the lid resting upside down on top, microwave 40.ml of Ammoniacal Silver Solution at for 30 seconds on HIGH power. 6. Using a plastic disposable pipet, vigorously stir the solution for a 3 seconds. 7. Place the slides in the hot silver solution 8. Replace the lid upside down on top of the Coplin jar and microwave for an additional 20 seconds on power 5. 9. For fungus, let the slides stand for exactly 1 minute following the last microwave step. For pneumocystis, let the slides sit for exactly 2 minutes following the last microwave step/ 10. Wash in 5 changes of DI Water 11. With slides lying flat, dispense enough Gold Chloride to cover the slide for 1 minute. Rinse in DI Water. 12. With slides lying flat, dispense enough Sodium Thiosulfate solution to cover the slide for 2 minutes. Rinse in DI Water. 13. With slides lying flat, dispense enough Light Green to cover the slide for 1 minute. Rinse in DI Water. 14. Dehydrate through graded alcohols, clear in 2 changes of Xylene and mount with a compatible mounting media. NOTE: Microwaves differ in power output. This procedure is based on our current microwave. If the power changes or the microwave is changed, then the timing of this procedure may have to be adjusted and the stain revalidated. ? RESULTS Fungi ? Black Pneumocystis ? Black to blue black Mucin ? Pale Gray Background - Green REFERENCE: Kit Procedure Insert, Rapid GMS for Fungus and Pneumocystis Carinii, Cat# k101, Poly Scientific R&D Corporation, Bay Shore NY 11706 Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 ********************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From cmconway <@t> usgs.gov Fri Jun 6 09:15:03 2014 From: cmconway <@t> usgs.gov (Conway, Carla) Date: Fri Jun 6 09:15:27 2014 Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Message-ID: Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov From Ashley.Troutman <@t> Vanderbilt.Edu Fri Jun 6 09:41:00 2014 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Jun 6 09:41:07 2014 Subject: [Histonet] RE: Churukian Silver stain for Fungus Message-ID: In my experience, periodic acid will not help you demonstrate histoplasma. Chromic acid should be the preferred oxidizer if histoplasmosis is suspected. I have had good results with periodic acid for pneumocystis and aspergillus, but not histoplasma. Ashley Troutman BS, MBA, HT(ASCP)QIHC Supervisor-Translational Pathology Shared Resource Vanderbilt University Medical Center S-1310 Medical Center North 1161 21st Avenue South Nashville, TN 37232 Message: 11 Date: Thu, 5 Jun 2014 09:29:29 -0400 From: "Terri Braud" > Subject: [Histonet] RE: Churukian Silver stain for Fungus To: "Tony Henwood (SCHN)" >, > Message-ID: > Content-Type: text/plain; charset="us-ascii" I beg to differ. I think you are confusing stains. I am not talking about using a Periodic Acid Schiff's stain (PAS) to stain fungus. I am referring to the Churukian Microwave Ammoniacal Silver stain for fungus. It oxidizes with Periodic Acid and the Silver solution is similar to what is used for most Reticulum stains. It stains the exact same organisms as a Grocott's Methenamine Silver (GMS), just without staining the elastic fibers. Our pneumocystis stains using the Churukian Ammoniacal Silver stain are just beautiful. This method has been taught at NSH workshops and has been widely used in published literature. As soon as I can figure out how to post pictures, I will send some pictures of a a pneumocystis control and an aspergillus control stained with the Churukian method. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Wednesday, June 04, 2014 7:20 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: Acid Clean Glassware No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience is that they are GMS (using chromic acid) negative. It is possible that our pathologists aren't aware that the "GMS" that their lab does might be using Periodic acid instead of Chromic acid. Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of chromic acid. The literature often does not report the exact GMS used which makes clear understanding of the histochemical results difficult. Pneumocystis will not be easy to see unless chromic acid is used (the mucin stains strongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 5 June 2014 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acid Clean Glassware Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: From algranth <@t> email.arizona.edu Fri Jun 6 10:27:42 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Fri Jun 6 10:27:51 2014 Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours In-Reply-To: References: Message-ID: ________Carla, I don't think you can be a real histotech if something like this has never happened to you! Have you figured out the cause of the malfunction? There are recipes that you can find online and probably on HISTONET for solutions to soak your tissues in to rehydrate them. You might want to try a lesser strength alcohol and move up to 70% before processing. Good luck. Hopefully the tissues won't turn out to be too difficult to section. If they are soak them in a little water with glycerin to soften them before cutting. Andi 96 days and counting until that retirement day! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Conway, Carla [cmconway@usgs.gov] Sent: Friday, June 06, 2014 7:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tp2 <@t> medicine.wisc.edu Fri Jun 6 10:53:03 2014 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Fri Jun 6 10:53:21 2014 Subject: [Histonet] DAB away alternative Message-ID: <53919D8F020000DF0000E378@gwmail2.medicine.wisc.edu> Hello Histonet, We have been using Lab Vision's DAB away to clean our Autostainer 360. However it seems a little wasteful to buy something that we can't use up before it expires. Do any of you have any exerpience with lower cost alternatives? Thanks, Tom From MMargiotta <@t> bmhmc.org Fri Jun 6 11:04:55 2014 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Fri Jun 6 11:05:03 2014 Subject: [Histonet] paraffin dispenser Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC2A5E2C87@BMH-EXCHANGE-01.BMHMC.ORG> Hi All, Anyone have a good recommendation for a reliable paraffin dispenser? The spigot keeps clogging on the one we have now. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From rmire <@t> cvpath.org Fri Jun 6 11:22:00 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Fri Jun 6 11:22:05 2014 Subject: [Histonet] cassette tissue baskets for Sakura VIP6 Message-ID: Hello Histonetters, Does anyone in Histoland have an off market source for the metal tissue processing baskets used in the Sakura VIP processor? I need to purchase several of the 150 cassette capacity and several of the 75 cassette capacity. They are super expensive directly from Sakura. Ronda Mire HT (ASCP) Laboratory Manager CVPath Institute Gaithersburg, MD From billodonnell <@t> catholichealth.net Fri Jun 6 11:22:21 2014 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Jun 6 11:22:27 2014 Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours In-Reply-To: References: Message-ID: Not since 1978. (Sorry - couldn't resist - Happy Friday!) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Friday, June 06, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=%2BfCU3trcgbZTxcTVVwV9PBYwA4sdkdeS3%2B8QsbqJBIg%3D%0A&s=f70156bac2d9c01ccd25a40b1c3fd9f124a0fe93c1fba0c52d4e1ef48da7a09e This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From mtighe <@t> trudeauinstitute.org Fri Jun 6 12:52:27 2014 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Fri Jun 6 12:52:36 2014 Subject: [Histonet] Processor problem Message-ID: <1402077076740.7582@trudeauinstitute.org> I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning. I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself. Has anybody had this problem? Thanks for any help! Mike From POWELL_SA <@t> mercer.edu Fri Jun 6 12:53:07 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Jun 6 12:53:14 2014 Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF92F5E81@MERCERMAIL.MercerU.local> Oh where is the like button. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 06, 2014 12:22 PM To: Conway, Carla; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Not since 1978. (Sorry - couldn't resist - Happy Friday!) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Friday, June 06, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=%2BfCU3trcgbZTxcTVVwV9PBYwA4sdkdeS3%2B8QsbqJBIg%3D%0A&s=f70156bac2d9c01ccd25a40b1c3fd9f124a0fe93c1fba0c52d4e1ef48da7a09e This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From CDavis <@t> che-east.org Fri Jun 6 13:31:45 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Fri Jun 6 13:31:59 2014 Subject: [Histonet] paraffin dispenser In-Reply-To: <3991bc48-b150-49b3-8db2-08aaaa07dae7@CHESXH02.one.ads.che.org> References: <3991bc48-b150-49b3-8db2-08aaaa07dae7@CHESXH02.one.ads.che.org> Message-ID: I recommend a slide incubator and a metal cup or two. The paraffin dispenser we purchased seem to be a relicensed commercial coffee pot. The spigot keeps clogging because they did not design it in a way that it would stay warm so, the paraffin cools in it and clogs. Cassie D. Michele" Subject: [Histonet] paraffin dispenser Message: 1 Date: Fri, 6 Jun 2014 16:04:55 +0000 From: "Margiotta-Watz, Michele" Subject: [Histonet] paraffin dispenser To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC2A5E2C87@BMH-EXCHANGE-01.BMHMC.ORG> Content-Type: text/plain; charset="us-ascii" Hi All, Anyone have a good recommendation for a reliable paraffin dispenser? The spigot keeps clogging on the one we have now. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jsjurczak <@t> comcast.net Fri Jun 6 13:43:13 2014 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Fri Jun 6 13:43:33 2014 Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25BF92F5E81@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE25BF92F5E81@MERCERMAIL.MercerU.local> Message-ID: <2129120167.2182515.1402080193013.JavaMail.root@comcast.net> Yet another reason to go microwave. This can't happen. ----- Original Message ----- From: "Shirley A. Powell" To: "Bill O'Donnell" , "Carla Conway" , histonet@lists.utsouthwestern.edu Sent: Friday, June 6, 2014 12:53:07 PM Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Oh where is the like button. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 06, 2014 12:22 PM To: Conway, Carla; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Not since 1978. (Sorry - couldn't resist - Happy Friday!) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Friday, June 06, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=%2BfCU3trcgbZTxcTVVwV9PBYwA4sdkdeS3%2B8QsbqJBIg%3D%0A&s=f70156bac2d9c01ccd25a40b1c3fd9f124a0fe93c1fba0c52d4e1ef48da7a09e This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 6 15:56:15 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 6 15:59:01 2014 Subject: [Histonet] Processor problem In-Reply-To: <1402077076740.7582@trudeauinstitute.org> References: <1402077076740.7582@trudeauinstitute.org> Message-ID: <1402088175.66031.YahooMailNeo@web120405.mail.ne1.yahoo.com> My advise is to call Sakura and schedule a maintenance visit. The rotary valve may be "acting up". Ren? J. On Friday, June 6, 2014 1:53 PM, Mike Tighe wrote: I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning. I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself. Has anybody had this problem? Thanks for any help! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Denice.Stiner <@t> nahealth.com Fri Jun 6 16:19:53 2014 From: Denice.Stiner <@t> nahealth.com (Denice Stiner) Date: Fri Jun 6 16:26:13 2014 Subject: [Histonet] Processor problem In-Reply-To: <1402088175.66031.YahooMailNeo@web120405.mail.ne1.yahoo.com> References: <1402077076740.7582@trudeauinstitute.org> <1402088175.66031.YahooMailNeo@web120405.mail.ne1.yahoo.com> Message-ID: I do not have a Tissue Tek 3000 but it sounds like maybe there is a sensor that needs wiped clean. Clearly it's not ready the fluid level in station 3 and returning it back to 1 for safe keeping. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 06, 2014 1:56 PM To: Mike Tighe; histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Processor problem My advise is to call Sakura and schedule a maintenance visit. The rotary valve may be "acting up". Ren? J. On Friday, June 6, 2014 1:53 PM, Mike Tighe wrote: I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning. I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself. Has anybody had this problem? Thanks for any help! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mitchell <@t> wanpost.com Sat Jun 7 03:21:43 2014 From: mitchell <@t> wanpost.com (Mitchell Wan (Wanpost)) Date: Sat Jun 7 03:15:01 2014 Subject: [Histonet] VIP3000 reagent bottle Message-ID: Hi All, Does anyone know where I can purchase some spare vip3000 reagent bottles? Regards Mitchell Wan 0418 745 750 P.O. BOX 2200, Runcorn,? Brisbane, QLD 4113 From mitchell <@t> wanpost.com Sat Jun 7 15:08:04 2014 From: mitchell <@t> wanpost.com (Mitchell Wan (Wanpost)) Date: Sat Jun 7 15:01:32 2014 Subject: [Histonet] Processor problem Message-ID: Hi Mike, Edit the 'dummy' run and make station 3 with '0' time. It is time based and has a safety mechanism. That's why it pumps the previous station. Then test again. A couple things may be an issue and need to be tested. 1. A hole, leak in the line of station 3. 2. Volume in reservoir bottle is too high 3. Kink in tube 4. Valve 'sticking'. Do the verify 7,8 test to hear the valve click in and out. A drop of xylene will fix this. Do this under supervision.? 5. Check valve pressure and vacuum. View from behind and see the guage. 6. Clog in line 3. Do a hot water flush. 2-3 times. Regards Mitchell Wan 0418 745 750 P.O. BOX 2200, Runcorn,? Brisbane, QLD 4113 -------- Original message -------- From: histonet-request@lists.utsouthwestern.edu Date:08/06/2014 3:03 AM (GMT+10:00) To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ?? 1. Processor problem (Mike Tighe) ?? 2. RE: Processor malfunction - tissues not submerged ~ 9 hours ????? (Shirley A. Powell) ?? 3. paraffin dispenser (Davis, Cassie) ?? 4. Re: Processor malfunction - tissues not submerged ~ 9 hours ????? (jsjurczak@comcast.net) ?? 5. Re: Processor problem (Rene J Buesa) ?? 6. RE: Processor problem (Denice Stiner) ?? 7. VIP3000 reagent bottle (Mitchell Wan (Wanpost)) ---------------------------------------------------------------------- Message: 1 Date: Fri, 6 Jun 2014 17:52:27 +0000 From: Mike Tighe Subject: [Histonet] Processor problem To: "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Message-ID: <1402077076740.7582@trudeauinstitute.org> Content-Type: text/plain; charset="iso-8859-1" I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning. I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself. Has anybody had this problem? Thanks for any help! Mike ------------------------------ Message: 2 Date: Fri, 6 Jun 2014 13:53:07 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours To: "O'Donnell, Bill" , "Conway, Carla" , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF92F5E81@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="utf-8" Oh where is the like button.? :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 06, 2014 12:22 PM To: Conway, Carla; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Not since 1978. (Sorry - couldn't resist - Happy Friday!) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Friday, June 06, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=%2BfCU3trcgbZTxcTVVwV9PBYwA4sdkdeS3%2B8QsbqJBIg%3D%0A&s=f70156bac2d9c01ccd25a40b1c3fd9f124a0fe93c1fba0c52d4e1ef48da7a09e This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 3 Date: Fri, 6 Jun 2014 14:31:45 -0400 From: "Davis, Cassie" Subject: [Histonet] paraffin dispenser To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I recommend a slide incubator and a metal cup or two. The paraffin dispenser we purchased seem to be a relicensed commercial coffee pot. The spigot keeps clogging because they did not design it in a way that it would stay warm so, the paraffin cools in it and clogs. Cassie D. Michele" Subject: [Histonet] paraffin dispenser Message: 1 Date: Fri, 6 Jun 2014 16:04:55 +0000 From: "Margiotta-Watz, Michele" Subject: [Histonet] paraffin dispenser To: "'histonet@lists.utsouthwestern.edu'" ??????? Message-ID: ??????? <230D0B9EC57D7A45A7A186C6AB4C7ABC2A5E2C87@BMH-EXCHANGE-01.BMHMC.ORG> Content-Type: text/plain; charset="us-ascii" Hi All, Anyone have a good recommendation for a reliable paraffin dispenser? The spigot keeps clogging on the one we have now. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed.? This communication may contain material protected by the attorney-client privilege.? If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.? If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Fri, 6 Jun 2014 18:43:13 +0000 (UTC) From: jsjurczak@comcast.net Subject: Re: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours To: "Shirley A. Powell" Cc: histonet@lists.utsouthwestern.edu Message-ID: <2129120167.2182515.1402080193013.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 Yet another reason to go microwave. This can't happen. ----- Original Message ----- From: "Shirley A. Powell" To: "Bill O'Donnell" , "Carla Conway" , histonet@lists.utsouthwestern.edu Sent: Friday, June 6, 2014 12:53:07 PM Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Oh where is the like button. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 06, 2014 12:22 PM To: Conway, Carla; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Not since 1978. (Sorry - couldn't resist - Happy Friday!) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Friday, June 06, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=%2BfCU3trcgbZTxcTVVwV9PBYwA4sdkdeS3%2B8QsbqJBIg%3D%0A&s=f70156bac2d9c01ccd25a40b1c3fd9f124a0fe93c1fba0c52d4e1ef48da7a09e This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 6 Jun 2014 13:56:15 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Processor problem To: Mike Tighe , "histonet@lists.utsouthwestern.edu \(histonet@lists.utsouthwestern.edu\)" Message-ID: <1402088175.66031.YahooMailNeo@web120405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My advise is to call Sakura and schedule a maintenance visit. The rotary valve may be "acting up". Ren? J. On Friday, June 6, 2014 1:53 PM, Mike Tighe wrote: ? I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning. I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself. Has anybody had this problem? Thanks for any help! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 6 Jun 2014 14:19:53 -0700 From: Denice Stiner Subject: RE: [Histonet] Processor problem To: Rene J Buesa , Mike Tighe , "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I do not have a Tissue Tek 3000 but it sounds like maybe there is a sensor that needs wiped clean.? Clearly it's not ready the fluid level in station 3 and returning it back to 1 for safe keeping. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 06, 2014 1:56 PM To: Mike Tighe; histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Processor problem My advise is to call Sakura and schedule a maintenance visit. The rotary valve may be "acting up". Ren? J. On Friday, June 6, 2014 1:53 PM, Mike Tighe wrote: ? I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning. I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself. Has anybody had this problem? Thanks for any help! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Sat, 07 Jun 2014 18:21:43 +1000 From: "Mitchell Wan (Wanpost)" Subject: [Histonet] VIP3000 reagent bottle To: HistoNET Message-ID: Content-Type: text/plain; charset=utf-8 Hi All, Does anyone know where I can purchase some spare vip3000 reagent bottles? Regards Mitchell Wan 0418 745 750 P.O. BOX 2200, Runcorn,?? Brisbane, QLD 4113 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 10 ***************************************** From ricca.dario <@t> gmail.com Mon Jun 9 05:02:16 2014 From: ricca.dario <@t> gmail.com (Dario Ricca) Date: Mon Jun 9 05:02:22 2014 Subject: [Histonet] DAB away alternative In-Reply-To: <53919D8F020000DF0000E378@gwmail2.medicine.wisc.edu> References: <53919D8F020000DF0000E378@gwmail2.medicine.wisc.edu> Message-ID: Hi Thomas, I've see that Dako's DAB Away is composed of 1% Oxalic acid in water instead other DAB Aways (like the one you are using) are composed of sulfuric acid <10% in water. Dario 2014-06-06 17:53 GMT+02:00 Thomas Pier : > Hello Histonet, > We have been using Lab Vision's DAB away to clean our Autostainer 360. > However it seems a little wasteful to buy something that we can't use up > before it expires. Do any of you have any exerpience with lower cost > alternatives? > > Thanks, > Tom > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Kathy.Machado <@t> LPNT.net Mon Jun 9 08:14:42 2014 From: Kathy.Machado <@t> LPNT.net (Kathy.Machado@LPNT.net) Date: Mon Jun 9 08:14:49 2014 Subject: [Histonet] G.I. biopsies and special stains Message-ID: I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 From mjones <@t> metropath.com Mon Jun 9 08:37:42 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Jun 9 08:38:07 2014 Subject: [Histonet] G.I. biopsies and special stains Message-ID: We order HP by IHC automatically on all stomach and GE juction specimens. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/9/14 7:14 AM, "Kathy.Machado@LPNT.net" wrote: >I was wondering if other labs are "automatically" ordering special stains >and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait >until H&E is screened? Thanks! > >Danville Regional Medical Center >Danville, VA >Kathy.Machado@lpnt.net >434-799-3868 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Mon Jun 9 08:42:23 2014 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Jun 9 08:42:29 2014 Subject: [Histonet] RE: G.I. biopsies and special stains In-Reply-To: References: Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB793@isexstore03> We automatically order CD3 and ALC BL/PAS on small bowel, ALC/BL PAS on Barrett's esophagus. H.P. on stomach. Per our Pathologist's order. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Machado@LPNT.net Sent: Monday, June 09, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From suetp918 <@t> comcast.net Mon Jun 9 09:01:12 2014 From: suetp918 <@t> comcast.net (Sue) Date: Mon Jun 9 09:01:54 2014 Subject: [Histonet] G.I. biopsies and special stains In-Reply-To: References: Message-ID: <2450D699-E1C5-45D6-8B4F-BC8C7FA41877@comcast.net> We auto do hp on gastric. Anything else is ordered by path. u need to be careful with ins. if neg the may not re-emburse. Sent from my iPhone > On Jun 9, 2014, at 9:14 AM, wrote: > > I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! > > Danville Regional Medical Center > Danville, VA > Kathy.Machado@lpnt.net > 434-799-3868 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Mon Jun 9 09:05:01 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Jun 9 09:05:09 2014 Subject: [Histonet] RE: G.I. biopsies and special stains In-Reply-To: References: Message-ID: H. Pylori by IHC on all stomach biopsies and Alcian Blue on all esophageal biopsies done automatically. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Machado@LPNT.net Sent: Monday, June 09, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From DKBoyd <@t> chs.net Mon Jun 9 09:05:05 2014 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Mon Jun 9 09:06:05 2014 Subject: [Histonet] RE: G.I. biopsies and special stains In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9541875@TN001WEXMBX12.US.chs.net> We have just discontinued this practice after Jane Pine Wood (renowned Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E stain is not reasonable and necessary". According to the pathologist billing company ,there is in an article they have privy to with wording which threatens "additional action" taken for providers whose ordering of special stains exceeds the 20th percentile of Gastric Pathology cases. For this reason we are now retro ordering special stains on gastric biopsies. We are also looking at IHC necessity. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] Sent: Monday, June 09, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From mpence <@t> grhs.net Mon Jun 9 09:11:05 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jun 9 09:11:31 2014 Subject: [Histonet] RE: G.I. biopsies and special stains In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246A9541875@TN001WEXMBX12.US.chs.net> References: <7EAFE982E328304DA6CE2B677BB76246A9541875@TN001WEXMBX12.US.chs.net> Message-ID: You gotta love Obamacare! Where "Legal meets Medical". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Monday, June 09, 2014 9:05 AM To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: G.I. biopsies and special stains We have just discontinued this practice after Jane Pine Wood (renowned Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E stain is not reasonable and necessary". According to the pathologist billing company ,there is in an article they have privy to with wording which threatens "additional action" taken for providers whose ordering of special stains exceeds the 20th percentile of Gastric Pathology cases. For this reason we are now retro ordering special stains on gastric biopsies. We are also looking at IHC necessity. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] Sent: Monday, June 09, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Mon Jun 9 09:14:47 2014 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Jun 9 09:14:50 2014 Subject: [Histonet] RE: G.I. biopsies and special stains In-Reply-To: References: <7EAFE982E328304DA6CE2B677BB76246A9541875@TN001WEXMBX12.US.chs.net> Message-ID: AKA - managed care. Emphasis on managed. On Jun 9, 2014 10:11 AM, "Mike Pence" wrote: > You gotta love Obamacare! Where "Legal meets Medical". > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M > Sent: Monday, June 09, 2014 9:05 AM > To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: G.I. biopsies and special stains > > We have just discontinued this practice after Jane Pine Wood (renowned > Healthcare Attorney and Medical Director for Palmetto Medicare Part B > coving NC, SC, VA, and WV) stated "ordering special stains prior to review > of the H&E stain is not reasonable and necessary". According to the > pathologist billing company ,there is in an article they have privy to with > wording which threatens "additional action" taken for providers whose > ordering of special stains exceeds the 20th percentile of Gastric Pathology > cases. For this reason we are now retro ordering special stains on gastric > biopsies. We are also looking at IHC necessity. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical > Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH > 804-765-5050 l FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of > Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] > Sent: Monday, June 09, 2014 9:14 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] G.I. biopsies and special stains > > I was wondering if other labs are "automatically" ordering special stains > and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait > until H&E is screened? Thanks! > > Danville Regional Medical Center > Danville, VA > Kathy.Machado@lpnt.net > 434-799-3868 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -------------------------------------------------------------------------- > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mjones <@t> metropath.com Mon Jun 9 09:33:05 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Jun 9 09:33:17 2014 Subject: [Histonet] G.I. biopsies and special stains In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24E4CE97@VAP1013.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24E4CE97@VAP1013.win.rwjuh.edu> Message-ID: We bill all of the stomach HP, but we do not bill all of the GE specimens. Only those deemed necessary (if we even do them - some pathologists do not want them, some do) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/9/14 8:30 AM, "Rathborne, Toni" wrote: >We wait for H&E. >For the labs that order automatically, do you bill all of them, or just >the ones determined to be necessary? > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael >Ann Jones >Sent: Monday, June 09, 2014 9:38 AM >To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] G.I. biopsies and special stains > >We order HP by IHC automatically on all stomach and GE juction specimens. >Michael Ann Jones, HT (ASCP) >Histology Manager >Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 6/9/14 7:14 AM, "Kathy.Machado@LPNT.net" >wrote: > >>I was wondering if other labs are "automatically" ordering special >>stains and/or IHC on stomach biopsies and esophageal biopsies? Or do >>you wait until H&E is screened? Thanks! >> >>Danville Regional Medical Center >>Danville, VA >>Kathy.Machado@lpnt.net >>434-799-3868 >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histo <@t> pathlab.us Mon Jun 9 09:33:01 2014 From: histo <@t> pathlab.us (Histology) Date: Mon Jun 9 09:33:42 2014 Subject: [Histonet] Documenting special stain control blocks Message-ID: <566E7795BC94204D9A21539DE282AED709A370@DC01.dp.local> Hi All, Just wondering how everyone is handling the new checklist question ANP. 21450? How are you documenting results of verified special stain tissue blocks? Thanks for any help, From abadesuyi <@t> nrh-ok.com Mon Jun 9 10:03:32 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Mon Jun 9 10:03:43 2014 Subject: [Histonet] Special Stainers Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82215D@MAIL.nrhnt.nrh-ok.com> Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From abadesuyi <@t> nrh-ok.com Mon Jun 9 10:27:35 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Mon Jun 9 10:27:40 2014 Subject: [Histonet] FW:H & E Stainers Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82215E@MAIL.nrhnt.nrh-ok.com> ________________________________ From: Adesupo, Adesuyi (Banjo) Sent: Monday, June 09, 2014 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Special Stainers Hi Guys, Please we are in the process of buying a new H & E Stainers and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From rjbuesa <@t> yahoo.com Mon Jun 9 10:53:10 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 9 10:56:03 2014 Subject: [Histonet] Special Stainers In-Reply-To: <04EE4F75BB5FB246ADB68D69B74604438E3B82215D@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B74604438E3B82215D@MAIL.nrhnt.nrh-ok.com> Message-ID: <1402329190.688.YahooMailNeo@web120403.mail.ne1.yahoo.com> Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, ? ? ? ? ? ? Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1.? Symphony by ventana 1.? TissueTek? Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, ? Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS ? Histology Supervisor ? Norman Regional Health System, ? Norman, OK 73071. ? Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Mon Jun 9 11:00:56 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Mon Jun 9 11:01:15 2014 Subject: [Histonet] Special Stainers In-Reply-To: <1402329190.688.YahooMailNeo@web120403.mail.ne1.yahoo.com> References: <04EE4F75BB5FB246ADB68D69B74604438E3B82215D@MAIL.nrhnt.nrh-ok.com>, <1402329190.688.YahooMailNeo@web120403.mail.ne1.yahoo.com> Message-ID: <1402329636943.16774@accuratediagnosticlabs.com> You cannot go wrong with the Prisma. I used that in another lab and it was wonderful. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Monday, June 09, 2014 2:53 PM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special Stainers Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Mon Jun 9 11:15:53 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Mon Jun 9 11:16:04 2014 Subject: [Histonet] Special Stainers In-Reply-To: <1402329636943.16774@accuratediagnosticlabs.com> References: <04EE4F75BB5FB246ADB68D69B74604438E3B82215D@MAIL.nrhnt.nrh-ok.com>, <1402329190.688.YahooMailNeo@web120403.mail.ne1.yahoo.com> <1402329636943.16774@accuratediagnosticlabs.com> Message-ID: The Ventana Symphony is undoubtedly the easiest stainer to use and maintain. You will never have an employee put an incorrect reagent into a container, and direct to drain waste makes it an appealing option. The H&E's are absolutely beautiful! Symphony is finicky about certain things though--the type of alcohol you use, the humidity levels in your lab, and the unit just doesn't like charged slides. Bubbles in coverslips and slides sticking together when filing may or may not be an issue. That being said, I've used Prisma's predecessors (the DRS 2000) and, no matter what, those things NEVER failed. We had three of them that ran 50-70 runs per day for around 10 years, and I can count on one hand the number of times they weren't functional. These are open container stainers though, and do require diligent maintenance and reagent changes/rotations. If you can handle that, I'd say go with the Prisma. Thanks, Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Monday, June 09, 2014 9:01 AM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu; Rene J Buesa Subject: RE: [Histonet] Special Stainers You cannot go wrong with the Prisma. I used that in another lab and it was wonderful. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Monday, June 09, 2014 2:53 PM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special Stainers Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jun 9 12:10:45 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jun 9 12:11:10 2014 Subject: [Histonet] Prisma stainer In-Reply-To: <6678405c-8356-4d5f-9fbf-ca8ec12d6a42@hpech1.HealthPartners.int> References: <6678405c-8356-4d5f-9fbf-ca8ec12d6a42@hpech1.HealthPartners.int> Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302E45CCEAF64@HPEMX3.HealthPartners.int> We have had the Prisma stainer for 2 years and LOVE it. Have never had a breakdown and it is a workhorse. It is user friendly and we do not have to use proprietary reagents, our choice! Dorothy Webb Regions Hospital Histology Technical Specialist -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, June 09, 2014 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: DAB away alternative (Dario Ricca) 2. G.I. biopsies and special stains (Kathy.Machado@LPNT.net) 3. Re: G.I. biopsies and special stains (Michael Ann Jones) 4. RE: G.I. biopsies and special stains (Hannen, Valerie) 5. Re: G.I. biopsies and special stains (Sue) 6. RE: G.I. biopsies and special stains (Tom McNemar) 7. RE: G.I. biopsies and special stains (Boyd, Debbie M) 8. RE: G.I. biopsies and special stains (Mike Pence) 9. Re: RE: G.I. biopsies and special stains (Victoria Baker) 10. Re: G.I. biopsies and special stains (Michael Ann Jones) 11. Documenting special stain control blocks (Histology) 12. Special Stainers (Adesupo, Adesuyi (Banjo)) 13. FW:H & E Stainers (Adesupo, Adesuyi (Banjo)) 14. Re: Special Stainers (Rene J Buesa) 15. RE: Special Stainers (Barbara Tibbs) 16. RE: Special Stainers (Cooper, Brian) ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Jun 2014 12:02:16 +0200 From: Dario Ricca Subject: Re: [Histonet] DAB away alternative To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi Thomas, I've see that Dako's DAB Away is composed of 1% Oxalic acid in water instead other DAB Aways (like the one you are using) are composed of sulfuric acid <10% in water. Dario 2014-06-06 17:53 GMT+02:00 Thomas Pier : > Hello Histonet, > We have been using Lab Vision's DAB away to clean our Autostainer 360. > However it seems a little wasteful to buy something that we can't use > up before it expires. Do any of you have any exerpience with lower > cost alternatives? > > Thanks, > Tom > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 2 Date: Mon, 9 Jun 2014 08:14:42 -0500 From: Subject: [Histonet] G.I. biopsies and special stains To: Message-ID: Content-Type: text/plain; charset="us-ascii" I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 ------------------------------ Message: 3 Date: Mon, 9 Jun 2014 13:37:42 +0000 From: Michael Ann Jones Subject: Re: [Histonet] G.I. biopsies and special stains To: "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We order HP by IHC automatically on all stomach and GE juction specimens. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/9/14 7:14 AM, "Kathy.Machado@LPNT.net" wrote: >I was wondering if other labs are "automatically" ordering special >stains and/or IHC on stomach biopsies and esophageal biopsies? Or do >you wait until H&E is screened? Thanks! > >Danville Regional Medical Center >Danville, VA >Kathy.Machado@lpnt.net >434-799-3868 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 9 Jun 2014 09:42:23 -0400 From: "Hannen, Valerie" Subject: [Histonet] RE: G.I. biopsies and special stains To: "'Kathy.Machado@LPNT.net'" , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB793@isexstore03> Content-Type: text/plain; charset="us-ascii" We automatically order CD3 and ALC BL/PAS on small bowel, ALC/BL PAS on Barrett's esophagus. H.P. on stomach. Per our Pathologist's order. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Machado@LPNT.net Sent: Monday, June 09, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 5 Date: Mon, 9 Jun 2014 10:01:12 -0400 From: Sue Subject: Re: [Histonet] G.I. biopsies and special stains To: "" Cc: "" Message-ID: <2450D699-E1C5-45D6-8B4F-BC8C7FA41877@comcast.net> Content-Type: text/plain; charset=us-ascii We auto do hp on gastric. Anything else is ordered by path. u need to be careful with ins. if neg the may not re-emburse. Sent from my iPhone > On Jun 9, 2014, at 9:14 AM, wrote: > > I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! > > Danville Regional Medical Center > Danville, VA > Kathy.Machado@lpnt.net > 434-799-3868 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 9 Jun 2014 10:05:01 -0400 From: Tom McNemar Subject: [Histonet] RE: G.I. biopsies and special stains To: "'Kathy.Machado@LPNT.net'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" H. Pylori by IHC on all stomach biopsies and Alcian Blue on all esophageal biopsies done automatically. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Machado@LPNT.net Sent: Monday, June 09, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 7 Date: Mon, 9 Jun 2014 14:05:05 +0000 From: "Boyd, Debbie M" Subject: [Histonet] RE: G.I. biopsies and special stains To: "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9541875@TN001WEXMBX12.US.chs.net> Content-Type: text/plain; charset="us-ascii" We have just discontinued this practice after Jane Pine Wood (renowned Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E stain is not reasonable and necessary". According to the pathologist billing company ,there is in an article they have privy to with wording which threatens "additional action" taken for providers whose ordering of special stains exceeds the 20th percentile of Gastric Pathology cases. For this reason we are now retro ordering special stains on gastric biopsies. We are also looking at IHC necessity. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] Sent: Monday, June 09, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 8 Date: Mon, 9 Jun 2014 14:11:05 +0000 From: Mike Pence Subject: [Histonet] RE: G.I. biopsies and special stains To: "'Boyd, Debbie M'" , "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" You gotta love Obamacare! Where "Legal meets Medical". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Monday, June 09, 2014 9:05 AM To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: G.I. biopsies and special stains We have just discontinued this practice after Jane Pine Wood (renowned Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E stain is not reasonable and necessary". According to the pathologist billing company ,there is in an article they have privy to with wording which threatens "additional action" taken for providers whose ordering of special stains exceeds the 20th percentile of Gastric Pathology cases. For this reason we are now retro ordering special stains on gastric biopsies. We are also looking at IHC necessity. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] Sent: Monday, June 09, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 9 Jun 2014 10:14:47 -0400 From: Victoria Baker Subject: Re: [Histonet] RE: G.I. biopsies and special stains To: Mike Pence Cc: "Boyd, Debbie M" , histonet netserver , "Kathy.Machado@LPNT.net" Message-ID: Content-Type: text/plain; charset=UTF-8 AKA - managed care. Emphasis on managed. On Jun 9, 2014 10:11 AM, "Mike Pence" wrote: > You gotta love Obamacare! Where "Legal meets Medical". > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M > Sent: Monday, June 09, 2014 9:05 AM > To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: G.I. biopsies and special stains > > We have just discontinued this practice after Jane Pine Wood (renowned > Healthcare Attorney and Medical Director for Palmetto Medicare Part B > coving NC, SC, VA, and WV) stated "ordering special stains prior to review > of the H&E stain is not reasonable and necessary". According to the > pathologist billing company ,there is in an article they have privy to > with wording which threatens "additional action" taken for providers > whose ordering of special stains exceeds the 20th percentile of > Gastric Pathology cases. For this reason we are now retro ordering > special stains on gastric biopsies. We are also looking at IHC necessity. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional > Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l > PH > 804-765-5050 l FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of > Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] > Sent: Monday, June 09, 2014 9:14 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] G.I. biopsies and special stains > > I was wondering if other labs are "automatically" ordering special > stains and/or IHC on stomach biopsies and esophageal biopsies? Or do > you wait until H&E is screened? Thanks! > > Danville Regional Medical Center > Danville, VA > Kathy.Machado@lpnt.net > 434-799-3868 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > ---- > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose > its contents or take any action in reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Mon, 9 Jun 2014 14:33:05 +0000 From: Michael Ann Jones Subject: Re: [Histonet] G.I. biopsies and special stains To: "Rathborne, Toni" , "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We bill all of the stomach HP, but we do not bill all of the GE specimens. Only those deemed necessary (if we even do them - some pathologists do not want them, some do) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/9/14 8:30 AM, "Rathborne, Toni" wrote: >We wait for H&E. >For the labs that order automatically, do you bill all of them, or just >the ones determined to be necessary? > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael >Ann Jones >Sent: Monday, June 09, 2014 9:38 AM >To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] G.I. biopsies and special stains > >We order HP by IHC automatically on all stomach and GE juction specimens. >Michael Ann Jones, HT (ASCP) >Histology Manager >Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 6/9/14 7:14 AM, "Kathy.Machado@LPNT.net" >wrote: > >>I was wondering if other labs are "automatically" ordering special >>stains and/or IHC on stomach biopsies and esophageal biopsies? Or do >>you wait until H&E is screened? Thanks! >> >>Danville Regional Medical Center >>Danville, VA >>Kathy.Machado@lpnt.net >>434-799-3868 >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 11 Date: Mon, 9 Jun 2014 14:33:01 +0000 From: Histology Subject: [Histonet] Documenting special stain control blocks To: "histonet@lists.utsouthwestern.edu" Message-ID: <566E7795BC94204D9A21539DE282AED709A370@DC01.dp.local> Content-Type: text/plain; charset="us-ascii" Hi All, Just wondering how everyone is handling the new checklist question ANP. 21450? How are you documenting results of verified special stain tissue blocks? Thanks for any help, ------------------------------ Message: 12 Date: Mon, 9 Jun 2014 10:03:32 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] Special Stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82215D@MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 13 Date: Mon, 9 Jun 2014 10:27:35 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] FW:H & E Stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82215E@MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" ________________________________ From: Adesupo, Adesuyi (Banjo) Sent: Monday, June 09, 2014 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Special Stainers Hi Guys, Please we are in the process of buying a new H & E Stainers and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 14 Date: Mon, 9 Jun 2014 08:53:10 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Special Stainers To: "Adesupo, Adesuyi \(Banjo\)" , "histonet@lists.utsouthwestern.edu" Message-ID: <1402329190.688.YahooMailNeo@web120403.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, ? ? ? ? ? ? Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1.? Symphony by ventana 1.? TissueTek? Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, ? Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS ? Histology Supervisor ? Norman Regional Health System, ? Norman, OK 73071. ? Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Mon, 9 Jun 2014 16:00:56 +0000 From: Barbara Tibbs Subject: RE: [Histonet] Special Stainers To: "Adesupo, Adesuyi (Banjo)" , "histonet@lists.utsouthwestern.edu" , "Rene J Buesa" Message-ID: <1402329636943.16774@accuratediagnosticlabs.com> Content-Type: text/plain; charset="iso-8859-1" You cannot go wrong with the Prisma. I used that in another lab and it was wonderful. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Monday, June 09, 2014 2:53 PM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special Stainers Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 9 Jun 2014 16:15:53 +0000 From: "Cooper, Brian" Subject: RE: [Histonet] Special Stainers To: "Adesupo, Adesuyi (Banjo)" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 The Ventana Symphony is undoubtedly the easiest stainer to use and maintain. You will never have an employee put an incorrect reagent into a container, and direct to drain waste makes it an appealing option. The H&E's are absolutely beautiful! Symphony is finicky about certain things though--the type of alcohol you use, the humidity levels in your lab, and the unit just doesn't like charged slides. Bubbles in coverslips and slides sticking together when filing may or may not be an issue. That being said, I've used Prisma's predecessors (the DRS 2000) and, no matter what, those things NEVER failed. We had three of them that ran 50-70 runs per day for around 10 years, and I can count on one hand the number of times they weren't functional. These are open container stainers though, and do require diligent maintenance and reagent changes/rotations. If you can handle that, I'd say go with the Prisma. Thanks, Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Monday, June 09, 2014 9:01 AM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu; Rene J Buesa Subject: RE: [Histonet] Special Stainers You cannot go wrong with the Prisma. I used that in another lab and it was wonderful. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Monday, June 09, 2014 2:53 PM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special Stainers Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 12 ***************************************** This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Kathy.Machado <@t> LPNT.net Mon Jun 9 12:21:19 2014 From: Kathy.Machado <@t> LPNT.net (Kathy.Machado@LPNT.net) Date: Mon Jun 9 12:21:25 2014 Subject: [Histonet] re: Documenting special stain control blocks Message-ID: I made up a form for the special stain/IHC controls for this that lists antibody/special stain, type of tissue, Case #, and date put in use. The pathologist looks at one stained slide, which is kept with the controls, and signs off on form. I hope this works!!! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 From barbara.tibbs <@t> accuratediagnosticlabs.com Mon Jun 9 12:32:59 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Mon Jun 9 12:33:10 2014 Subject: [Histonet] RE: re: Documenting special stain control blocks In-Reply-To: References: Message-ID: <1402335159745.96679@accuratediagnosticlabs.com> I did the same thing. We've had no problem passing inspections. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kathy.Machado@LPNT.net Sent: Monday, June 09, 2014 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: Documenting special stain control blocks I made up a form for the special stain/IHC controls for this that lists antibody/special stain, type of tissue, Case #, and date put in use. The pathologist looks at one stained slide, which is kept with the controls, and signs off on form. I hope this works!!! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abilger <@t> wellspan.org Mon Jun 9 12:50:08 2014 From: abilger <@t> wellspan.org (Bilger, Andrea) Date: Mon Jun 9 12:50:22 2014 Subject: [Histonet] GI biopsies and special stains Message-ID: <6f8c97843f59471c846733a7c26539db@BY2PR02MB300.namprd02.prod.outlook.com> Tests need to be medically necessary, therefore, we do not auto order stains on all GI biopsies. We wait until the H&E is screened and then order H Pylori by IHC on only those biopsies that need it. Andrea Bilger, HTL Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ From barbara.tibbs <@t> accuratediagnosticlabs.com Mon Jun 9 13:20:30 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Mon Jun 9 13:20:40 2014 Subject: [Histonet] RE: GI biopsies and special stains In-Reply-To: <6f8c97843f59471c846733a7c26539db@BY2PR02MB300.namprd02.prod.outlook.com> References: <6f8c97843f59471c846733a7c26539db@BY2PR02MB300.namprd02.prod.outlook.com> Message-ID: <1402338010779.53699@accuratediagnosticlabs.com> I agree. However, if you only receive a few GI biopsies a day that might me feasible. 95% of my work is GI biopsies. I live in an area with a very large immigrant population which tends to have h.pylori infection (from what I've seen). On any given day over 50% of the gastric biopsies could be h.pylori positive. We see pre-ordering special stains as being efficient and saving the pathologist some time as well as increasing turnaround time. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bilger, Andrea Sent: Monday, June 09, 2014 4:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsies and special stains Tests need to be medically necessary, therefore, we do not auto order stains on all GI biopsies. We wait until the H&E is screened and then order H Pylori by IHC on only those biopsies that need it. Andrea Bilger, HTL Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SSCALISE <@t> beaumont.edu Mon Jun 9 13:27:20 2014 From: SSCALISE <@t> beaumont.edu (Sharon Scalise) Date: Mon Jun 9 13:27:29 2014 Subject: [Histonet] RE: Prisma stainer In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302E45CCEAF64@HPEMX3.HealthPartners.int> References: <6678405c-8356-4d5f-9fbf-ca8ec12d6a42@hpech1.HealthPartners.int> <65365F35C0F2EF4D846EC3CA73E49C4302E45CCEAF64@HPEMX3.HealthPartners.int> Message-ID: <190D98228ADC1747BCE27019B78FD8F3011F91F7@EXMAIL06.ms.beaumont.edu> I am wondering what program you are using for a routine H&E stain. When are stainer was set-up we were told that each station had to be at least 2 minutes; if less than that the arm would "babysit" at a station until it moved it to a station that was 2 minutes or longer. This added about 12 minutes to our stain protocol and when we are busy in the morning it really slows down the staining runs. We are having a real issue with this so we are going to test the stainer with shorter times; if others already have shorter protocols that work could you please share them. (We stain between 600 and 1,000 slides per day) Sharon Scalise -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Monday, June 09, 2014 1:11 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Prisma stainer We have had the Prisma stainer for 2 years and LOVE it. Have never had a breakdown and it is a workhorse. It is user friendly and we do not have to use proprietary reagents, our choice! Dorothy Webb Regions Hospital Histology Technical Specialist -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, June 09, 2014 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: DAB away alternative (Dario Ricca) 2. G.I. biopsies and special stains (Kathy.Machado@LPNT.net) 3. Re: G.I. biopsies and special stains (Michael Ann Jones) 4. RE: G.I. biopsies and special stains (Hannen, Valerie) 5. Re: G.I. biopsies and special stains (Sue) 6. RE: G.I. biopsies and special stains (Tom McNemar) 7. RE: G.I. biopsies and special stains (Boyd, Debbie M) 8. RE: G.I. biopsies and special stains (Mike Pence) 9. Re: RE: G.I. biopsies and special stains (Victoria Baker) 10. Re: G.I. biopsies and special stains (Michael Ann Jones) 11. Documenting special stain control blocks (Histology) 12. Special Stainers (Adesupo, Adesuyi (Banjo)) 13. FW:H & E Stainers (Adesupo, Adesuyi (Banjo)) 14. Re: Special Stainers (Rene J Buesa) 15. RE: Special Stainers (Barbara Tibbs) 16. RE: Special Stainers (Cooper, Brian) ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Jun 2014 12:02:16 +0200 From: Dario Ricca Subject: Re: [Histonet] DAB away alternative To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi Thomas, I've see that Dako's DAB Away is composed of 1% Oxalic acid in water instead other DAB Aways (like the one you are using) are composed of sulfuric acid <10% in water. Dario 2014-06-06 17:53 GMT+02:00 Thomas Pier : > Hello Histonet, > We have been using Lab Vision's DAB away to clean our Autostainer 360. > However it seems a little wasteful to buy something that we can't use > up before it expires. Do any of you have any exerpience with lower > cost alternatives? > > Thanks, > Tom > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 2 Date: Mon, 9 Jun 2014 08:14:42 -0500 From: Subject: [Histonet] G.I. biopsies and special stains To: Message-ID: Content-Type: text/plain; charset="us-ascii" I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 ------------------------------ Message: 3 Date: Mon, 9 Jun 2014 13:37:42 +0000 From: Michael Ann Jones Subject: Re: [Histonet] G.I. biopsies and special stains To: "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We order HP by IHC automatically on all stomach and GE juction specimens. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/9/14 7:14 AM, "Kathy.Machado@LPNT.net" wrote: >I was wondering if other labs are "automatically" ordering special >stains and/or IHC on stomach biopsies and esophageal biopsies? Or do >you wait until H&E is screened? Thanks! > >Danville Regional Medical Center >Danville, VA >Kathy.Machado@lpnt.net >434-799-3868 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 9 Jun 2014 09:42:23 -0400 From: "Hannen, Valerie" Subject: [Histonet] RE: G.I. biopsies and special stains To: "'Kathy.Machado@LPNT.net'" , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB793@isexstore03> Content-Type: text/plain; charset="us-ascii" We automatically order CD3 and ALC BL/PAS on small bowel, ALC/BL PAS on Barrett's esophagus. H.P. on stomach. Per our Pathologist's order. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Machado@LPNT.net Sent: Monday, June 09, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 5 Date: Mon, 9 Jun 2014 10:01:12 -0400 From: Sue Subject: Re: [Histonet] G.I. biopsies and special stains To: "" Cc: "" Message-ID: <2450D699-E1C5-45D6-8B4F-BC8C7FA41877@comcast.net> Content-Type: text/plain; charset=us-ascii We auto do hp on gastric. Anything else is ordered by path. u need to be careful with ins. if neg the may not re-emburse. Sent from my iPhone > On Jun 9, 2014, at 9:14 AM, wrote: > > I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! > > Danville Regional Medical Center > Danville, VA > Kathy.Machado@lpnt.net > 434-799-3868 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 9 Jun 2014 10:05:01 -0400 From: Tom McNemar Subject: [Histonet] RE: G.I. biopsies and special stains To: "'Kathy.Machado@LPNT.net'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" H. Pylori by IHC on all stomach biopsies and Alcian Blue on all esophageal biopsies done automatically. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Machado@LPNT.net Sent: Monday, June 09, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 7 Date: Mon, 9 Jun 2014 14:05:05 +0000 From: "Boyd, Debbie M" Subject: [Histonet] RE: G.I. biopsies and special stains To: "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9541875@TN001WEXMBX12.US.chs.net> Content-Type: text/plain; charset="us-ascii" We have just discontinued this practice after Jane Pine Wood (renowned Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E stain is not reasonable and necessary". According to the pathologist billing company ,there is in an article they have privy to with wording which threatens "additional action" taken for providers whose ordering of special stains exceeds the 20th percentile of Gastric Pathology cases. For this reason we are now retro ordering special stains on gastric biopsies. We are also looking at IHC necessity. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] Sent: Monday, June 09, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 8 Date: Mon, 9 Jun 2014 14:11:05 +0000 From: Mike Pence Subject: [Histonet] RE: G.I. biopsies and special stains To: "'Boyd, Debbie M'" , "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" You gotta love Obamacare! Where "Legal meets Medical". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Monday, June 09, 2014 9:05 AM To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: G.I. biopsies and special stains We have just discontinued this practice after Jane Pine Wood (renowned Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E stain is not reasonable and necessary". According to the pathologist billing company ,there is in an article they have privy to with wording which threatens "additional action" taken for providers whose ordering of special stains exceeds the 20th percentile of Gastric Pathology cases. For this reason we are now retro ordering special stains on gastric biopsies. We are also looking at IHC necessity. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] Sent: Monday, June 09, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 9 Jun 2014 10:14:47 -0400 From: Victoria Baker Subject: Re: [Histonet] RE: G.I. biopsies and special stains To: Mike Pence Cc: "Boyd, Debbie M" , histonet netserver , "Kathy.Machado@LPNT.net" Message-ID: Content-Type: text/plain; charset=UTF-8 AKA - managed care. Emphasis on managed. On Jun 9, 2014 10:11 AM, "Mike Pence" wrote: > You gotta love Obamacare! Where "Legal meets Medical". > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M > Sent: Monday, June 09, 2014 9:05 AM > To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: G.I. biopsies and special stains > > We have just discontinued this practice after Jane Pine Wood (renowned > Healthcare Attorney and Medical Director for Palmetto Medicare Part B > coving NC, SC, VA, and WV) stated "ordering special stains prior to review > of the H&E stain is not reasonable and necessary". According to the > pathologist billing company ,there is in an article they have privy to > with wording which threatens "additional action" taken for providers > whose ordering of special stains exceeds the 20th percentile of > Gastric Pathology cases. For this reason we are now retro ordering > special stains on gastric biopsies. We are also looking at IHC necessity. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional > Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l > PH > 804-765-5050 l FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of > Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] > Sent: Monday, June 09, 2014 9:14 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] G.I. biopsies and special stains > > I was wondering if other labs are "automatically" ordering special > stains and/or IHC on stomach biopsies and esophageal biopsies? Or do > you wait until H&E is screened? Thanks! > > Danville Regional Medical Center > Danville, VA > Kathy.Machado@lpnt.net > 434-799-3868 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > ---- > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose > its contents or take any action in reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Mon, 9 Jun 2014 14:33:05 +0000 From: Michael Ann Jones Subject: Re: [Histonet] G.I. biopsies and special stains To: "Rathborne, Toni" , "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We bill all of the stomach HP, but we do not bill all of the GE specimens. Only those deemed necessary (if we even do them - some pathologists do not want them, some do) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/9/14 8:30 AM, "Rathborne, Toni" wrote: >We wait for H&E. >For the labs that order automatically, do you bill all of them, or just >the ones determined to be necessary? > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael >Ann Jones >Sent: Monday, June 09, 2014 9:38 AM >To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] G.I. biopsies and special stains > >We order HP by IHC automatically on all stomach and GE juction specimens. >Michael Ann Jones, HT (ASCP) >Histology Manager >Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 6/9/14 7:14 AM, "Kathy.Machado@LPNT.net" >wrote: > >>I was wondering if other labs are "automatically" ordering special >>stains and/or IHC on stomach biopsies and esophageal biopsies? Or do >>you wait until H&E is screened? Thanks! >> >>Danville Regional Medical Center >>Danville, VA >>Kathy.Machado@lpnt.net >>434-799-3868 >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 11 Date: Mon, 9 Jun 2014 14:33:01 +0000 From: Histology Subject: [Histonet] Documenting special stain control blocks To: "histonet@lists.utsouthwestern.edu" Message-ID: <566E7795BC94204D9A21539DE282AED709A370@DC01.dp.local> Content-Type: text/plain; charset="us-ascii" Hi All, Just wondering how everyone is handling the new checklist question ANP. 21450? How are you documenting results of verified special stain tissue blocks? Thanks for any help, ------------------------------ Message: 12 Date: Mon, 9 Jun 2014 10:03:32 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] Special Stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82215D@MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 13 Date: Mon, 9 Jun 2014 10:27:35 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] FW:H & E Stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82215E@MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" ________________________________ From: Adesupo, Adesuyi (Banjo) Sent: Monday, June 09, 2014 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Special Stainers Hi Guys, Please we are in the process of buying a new H & E Stainers and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 14 Date: Mon, 9 Jun 2014 08:53:10 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Special Stainers To: "Adesupo, Adesuyi \(Banjo\)" , "histonet@lists.utsouthwestern.edu" Message-ID: <1402329190.688.YahooMailNeo@web120403.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, ? ? ? ? ? ? Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1.? Symphony by ventana 1.? TissueTek? Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, ? Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS ? Histology Supervisor ? Norman Regional Health System, ? Norman, OK 73071. ? Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Mon, 9 Jun 2014 16:00:56 +0000 From: Barbara Tibbs Subject: RE: [Histonet] Special Stainers To: "Adesupo, Adesuyi (Banjo)" , "histonet@lists.utsouthwestern.edu" , "Rene J Buesa" Message-ID: <1402329636943.16774@accuratediagnosticlabs.com> Content-Type: text/plain; charset="iso-8859-1" You cannot go wrong with the Prisma. I used that in another lab and it was wonderful. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Monday, June 09, 2014 2:53 PM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special Stainers Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 9 Jun 2014 16:15:53 +0000 From: "Cooper, Brian" Subject: RE: [Histonet] Special Stainers To: "Adesupo, Adesuyi (Banjo)" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 The Ventana Symphony is undoubtedly the easiest stainer to use and maintain. You will never have an employee put an incorrect reagent into a container, and direct to drain waste makes it an appealing option. The H&E's are absolutely beautiful! Symphony is finicky about certain things though--the type of alcohol you use, the humidity levels in your lab, and the unit just doesn't like charged slides. Bubbles in coverslips and slides sticking together when filing may or may not be an issue. That being said, I've used Prisma's predecessors (the DRS 2000) and, no matter what, those things NEVER failed. We had three of them that ran 50-70 runs per day for around 10 years, and I can count on one hand the number of times they weren't functional. These are open container stainers though, and do require diligent maintenance and reagent changes/rotations. If you can handle that, I'd say go with the Prisma. Thanks, Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Monday, June 09, 2014 9:01 AM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu; Rene J Buesa Subject: RE: [Histonet] Special Stainers You cannot go wrong with the Prisma. I used that in another lab and it was wonderful. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Monday, June 09, 2014 2:53 PM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special Stainers Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 12 ***************************************** This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Jun 9 13:27:46 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jun 9 13:27:52 2014 Subject: [Histonet] RE: GI biopsies and special stains In-Reply-To: <6f8c97843f59471c846733a7c26539db@BY2PR02MB300.namprd02.prod.outlook.com> References: <6f8c97843f59471c846733a7c26539db@BY2PR02MB300.namprd02.prod.outlook.com> Message-ID: <25A4DE08332B19499904459F00AAACB719DE6DEE4C@EVS1.archildrens.org> We do the same as Andrea. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bilger, Andrea Sent: Monday, June 09, 2014 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GI biopsies and special stains Tests need to be medically necessary, therefore, we do not auto order stains on all GI biopsies. We wait until the H&E is screened and then order H Pylori by IHC on only those biopsies that need it. Andrea Bilger, HTL Team Leader, Histology York Hospital 1001 S. George St. York, Pa. 17405 (717) 851-5040 ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From september.amspacher <@t> bassett.org Mon Jun 9 13:31:21 2014 From: september.amspacher <@t> bassett.org (Amspacher, September) Date: Mon Jun 9 13:31:42 2014 Subject: [Histonet] Equipment for Sale Message-ID: I have a Microm HM550vp cryostat for sale. Please contact me if interested - vendor contacts welcome Victoria Spoon Anatomic Pathology Manager Point Of Care Manager victoria.spoon@bassett.org Tel(607) 547-6357 Fax(607) 547-3203 September Amspacher HT(ASCP) From delsuec <@t> gmail.com Mon Jun 9 13:48:44 2014 From: delsuec <@t> gmail.com (Deloris Carter) Date: Mon Jun 9 13:48:48 2014 Subject: [Histonet] Leica special/h&e stainer Message-ID: Does anyone have any feedback on the Leica Multistainer? It stains H&E as well as special stains. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center From cls71877 <@t> gmail.com Mon Jun 9 13:59:29 2014 From: cls71877 <@t> gmail.com (Cristi) Date: Mon Jun 9 13:59:34 2014 Subject: [Histonet] Leica special/h&e stainer In-Reply-To: References: Message-ID: The ST 5020? We have that machine and do like it. We are however a rather small volume lab with limited stain needs. I think if you are looking for a variety of staining protocols you should ask what the estimated time is if they are all running at the same time. We only do a dewax, H&E and DQ stain and we have to place the racks strategically if we are running behind. You can "dip" yourself into lengthy runs and long holding times in stations if you aren't careful. Mechanically it has been running with no issues for five years now. Please let me know if you have other more specific questions. Have a great day, Cristi Sent from my iPhone > On Jun 9, 2014, at 11:48 AM, Deloris Carter wrote: > > Does anyone have any feedback on the Leica Multistainer? It stains H&E as > well as special stains. > > Thanks, > Deloris Carter, HT(ASCP) > Shawnee Mission Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Mon Jun 9 14:06:43 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Mon Jun 9 14:06:49 2014 Subject: [Histonet] Leica special/h&e stainer In-Reply-To: References: Message-ID: <1402340783698.503@accuratediagnosticlabs.com> I have the Leica Multistainer. It was purchased before I got here. The only thing I don't like is that the programs have to be "compatible" or else the machine alarms when you load a special stain onto the machine when it already has an H&E going. There's a rather complicated way to get the programs to be compatible which was explained to me when I went for training. You have to use an excel spreadsheet and figure out what rinse is where during all the programs. Yes, it's something Leica's engineers have to work on. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deloris Carter Sent: Monday, June 09, 2014 5:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica special/h&e stainer Does anyone have any feedback on the Leica Multistainer? It stains H&E as well as special stains. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrie.schray <@t> gmail.com Mon Jun 9 14:30:27 2014 From: carrie.schray <@t> gmail.com (Carrie Schray) Date: Mon Jun 9 14:30:30 2014 Subject: [Histonet] Alpha A Crystallin Immuno Message-ID: Hello One of my vets would like to run Alpha A Crystallin IHC on rabbit eyes. Anyone out there tried this? Any recommendations? Thank you, Carrie Schray Univ. of Michigan Medical School Ann Arbor, MI From rsrichmond <@t> gmail.com Mon Jun 9 15:56:41 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Jun 9 15:56:45 2014 Subject: [Histonet] Re: G.I. biopsies and special stains Message-ID: Kathy Machado at Danville, Virginia, Regional Medical Center asks: >>I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened?<< A number of fairly small labs I've worked in in recent years all do whatever studies they do at all on all biopsies. What they do varies greatly - and usually the pathologist isn't allowed to ask for anything not done on every biopsy. Specifically: Every lab I've seen does either a stain or IHC for Helicobacter on all gastric biopsies. A few do Alcian blue on all esophageal biopsies. I've never seen a lab that did any stain routinely on duodenal biopsies, where the pathologist is deliriously happy just to get consistently interpretable H & E slides. I've been asking around about routine CD3 on duodenal biopsies. A fair number of labs do it, but G.I. pathologists caution you not to, because there are no published standards for interpreting this stain. As you're probably aware, the regulatory agency for the Virginias and the Carolinas is planning to put a stop to all routine G.I. biopsy special stains, and limit Helicobacter staining to 20% of gastric biopsies. I think this is acceptable practice, provided that there is a serious quality assurance program for G.I. biopsy slides, something I've yet to see. Bob Richmond Samurai Pathologist Maryville TN From jqb7 <@t> cdc.gov Mon Jun 9 17:23:37 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon Jun 9 17:23:55 2014 Subject: [Histonet] Leica special/h&e stainer In-Reply-To: <1402340783698.503@accuratediagnosticlabs.com> References: <1402340783698.503@accuratediagnosticlabs.com> Message-ID: <3B2CD438E1628A41BD687E98B963B7811FD267E4@EMBX-CHAM2.cdc.gov> We have one as well but the special stains function for us was just something we thought we might use...we did purchase the bucket inserts just in case. But it was incompatible with simultaneously ding H&E's so we bought a Dako Artisan just for specials and love it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Monday, June 09, 2014 3:07 PM To: Deloris Carter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica special/h&e stainer I have the Leica Multistainer. It was purchased before I got here. The only thing I don't like is that the programs have to be "compatible" or else the machine alarms when you load a special stain onto the machine when it already has an H&E going. There's a rather complicated way to get the programs to be compatible which was explained to me when I went for training. You have to use an excel spreadsheet and figure out what rinse is where during all the programs. Yes, it's something Leica's engineers have to work on. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deloris Carter Sent: Monday, June 09, 2014 5:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica special/h&e stainer Does anyone have any feedback on the Leica Multistainer? It stains H&E as well as special stains. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mikeykkk_3 <@t> live.com Mon Jun 9 17:42:05 2014 From: mikeykkk_3 <@t> live.com (Michael Backhus) Date: Mon Jun 9 17:42:19 2014 Subject: [Histonet] Leica special/h&e stainer In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FD267E4@EMBX-CHAM2.cdc.gov> References: <1402340783698.503@accuratediagnosticlabs.com> <3B2CD438E1628A41BD687E98B963B7811FD267E4@EMBX-CHAM2.cdc.gov> Message-ID: On GI we always did as follows: Duodenum H&E Antrum HP/AB Greater/Lesser Curvature HP/AB Esophagus AB/PAS GE Junction HP/AB/PAS If there were multiply esophagus sites at different centimeters we did specials on everyone. The HP was not a IHC I was always curious if this was too many stains. Mike Sent from my iPhone > On Jun 9, 2014, at 6:23 PM, "Sanders, Jeanine (CDC/OID/NCEZID)" wrote: > > We have one as well but the special stains function for us was just something we thought we might use...we did purchase the bucket inserts just in case. But it was incompatible with simultaneously ding H&E's so we bought a Dako Artisan just for specials and love it. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs > Sent: Monday, June 09, 2014 3:07 PM > To: Deloris Carter; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Leica special/h&e stainer > > I have the Leica Multistainer. It was purchased before I got here. The only thing I don't like is that the programs have to be "compatible" or else the machine alarms when you load a special stain onto the machine when it already has an H&E going. There's a rather complicated way to get the programs to be compatible which was explained to me when I went for training. You have to use an excel spreadsheet and figure out what rinse is where during all the programs. Yes, it's something Leica's engineers have to work on. > > Barbara S. Tibbs > Histology Supervisor > Accurate Diagnostic Labs > South Plainfield, NJ > barbara.tibbs@accuratediagnosticlabs.com > 732-839-3374 > Cell: 610-809-6508 > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deloris Carter > Sent: Monday, June 09, 2014 5:48 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica special/h&e stainer > > Does anyone have any feedback on the Leica Multistainer? It stains H&E as well as special stains. > > Thanks, > Deloris Carter, HT(ASCP) > Shawnee Mission Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tony.henwood <@t> health.nsw.gov.au Mon Jun 9 19:40:27 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jun 9 19:40:51 2014 Subject: [Histonet] RE: Churukian Silver stain for Fungus In-Reply-To: References: <20140604190246.71C7B1E808B@trendmess-svr.holyredeemer.local> <6D6BD1DE8A5571489398B392A38A7157E00EB0EC@xmdb04.nch.kids> <6D6BD1DE8A5571489398B392A38A7157E00EB5D6@xmdb04.nch.kids> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00EBC9A@xmdb04.nch.kids> Hi Terri, I agree, Churukian's Ammoniacal silver method is very good. I assume you are referring to the method given in Churukian, C. J., & Schenk, E. A. (1988). Staining Pneumocystis carinii and fungi in unfixed specimens with ammoniacal silver using a microwave oven. Journal of Histotechnology, 11(1), 19-21. In their discussion they note: "Paraffin sections can also be stained with this method with only minor changes. In place of 5% periodic acid use 5% chromic acid" Though the reason given is that paraffin sections tend to detach from slides. Charles repeats this recommendation in his later article: Churukian, C. J. (1996). Consistent silver methods for demonstrating basement membranes, reticulum, and fungi. Journal of histotechnology, 19(3), 211-217. I follow Charles recommendations for paraffin sections (ie chromic acid). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Terri Braud [mailto:tbraud@holyredeemer.com] Sent: Friday, 6 June 2014 10:50 PM To: Tony Henwood (SCHN) Subject: RE: Churukian Silver stain for Fungus Tony - I totally agree with you (and your references) that Periodic Acid is not as good when used with Grocott's methenamine silver as in the GMS. However, it totally rocks when used with the Churukian Ammoniacal Silver Method. We are still talking about 2 different staining methods. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Thursday, June 05, 2014 9:17 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: Churukian Silver stain for Fungus Terri, I was referring to the incorrect use of periodic acid in the GMS stain. The Grocott's Methenamine silver stain uses chromic acid, not Periodic acid. There are many instances in the literature where using a classic GMS has been found superior for demonstrating fungus rather than PAS. I am concerned that Periodic acid - silver staining has not been validated using cases where classic GMS has been shown to be more sensitive than PAS (see below) or in cases of pseudofungi, which are PAS positive but overall GMS negative. An appreciation of the difficulties in relying on Periodic acid rather than chromic acid for GMS staining can be seen in these articles: Chandler, F. W., & Watts, J. C. (1995). Fungal diseases. Journal of Histotechnology, 18(3), 247-252 Carson, F. L., Fredenburgh, J., & Maxwell, J. E. (1999). Inconsistent detection of histoplasma capsulatum with periodic acid oxidation in the Grocott methenamine-silver nitrate (GMS) fungus stain. Journal of histotechnology, 22(2), 119-122. Carson, F. L., & Richmond, R. S. (2010). Periodic acid cannot replace chromic acid in Grocott's method for fungi. Biotechnic & histochemistry: official publication of the Biological Stain Commission, 85(4), 270. Henwood, A. F., Prasad, L., & Bourke, V. M. (2013). The application of heated detergent dewaxing and rehydration to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing. Journal of Histotechnology, 36(2), 45-50. Song, D. E., Kahn, A. G., Khang, S. K., & Ro, J. Y. (2005). Pseudofungi in pericolic lymph nodes. Archives of pathology & laboratory medicine, 129(4), e97-e100. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Terri Braud [mailto:tbraud@holyredeemer.com] Sent: Thursday, 5 June 2014 11:29 PM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: RE: Churukian Silver stain for Fungus I beg to differ. I think you are confusing stains. I am not talking about using a Periodic Acid Schiff's stain (PAS) to stain fungus. I am referring to the Churukian Microwave Ammoniacal Silver stain for fungus. It oxidizes with Periodic Acid and the Silver solution is similar to what is used for most Reticulum stains. It stains the exact same organisms as a Grocott's Methenamine Silver (GMS), just without staining the elastic fibers. Our pneumocystis stains using the Churukian Ammoniacal Silver stain are just beautiful. This method has been taught at NSH workshops and has been widely used in published literature. As soon as I can figure out how to post pictures, I will send some pictures of a a pneumocystis control and an aspergillus control stained with the Churukian method. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood@health.nsw.gov.au] Sent: Wednesday, June 04, 2014 7:20 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: Acid Clean Glassware No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience is that they are GMS (using chromic acid) negative. It is possible that our pathologists aren't aware that the "GMS" that their lab does might be using Periodic acid instead of Chromic acid. Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of chromic acid. The literature often does not report the exact GMS used which makes clear understanding of the histochemical results difficult. Pneumocystis will not be easy to see unless chromic acid is used (the mucin stains strongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 5 June 2014 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acid Clean Glassware Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. Acid Cleaned glassware (Abbott, Tanya) ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ********* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ ********* ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ************************************************************************ ********* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ ********* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood <@t> health.nsw.gov.au Mon Jun 9 19:41:35 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jun 9 19:41:45 2014 Subject: [Histonet] RE: Churukian Silver stain for pneumocystis and fungus In-Reply-To: References: <20140605161638.7B2151E80FC@trendmess-svr.holyredeemer.local> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00EBCA3@xmdb04.nch.kids> Hi Terri, I agree, Churukian's Ammoniacal silver method is very good. I assume you are referring to the method given in Churukian, C. J., & Schenk, E. A. (1988). Staining Pneumocystis carinii and fungi in unfixed specimens with ammoniacal silver using a microwave oven. Journal of Histotechnology, 11(1), 19-21. In their discussion they note: "Paraffin sections can also be stained with this method with only minor changes. In place of 5% periodic acid use 5% chromic acid" Though the reason given is that paraffin sections tend to detach from slides. Charles repeats this recommendation in his later article: Churukian, C. J. (1996). Consistent silver methods for demonstrating basement membranes, reticulum, and fungi. Journal of histotechnology, 19(3), 211-217. I follow Charles recommendations for paraffin sections (ie chromic acid). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Friday, 6 June 2014 11:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Churukian Silver stain for pneumocystis and fungus For those who have written off list for the Churukian stain procedure, I've included it here. It is also sold in the USA in kit form by Polyscientific (no plug here, just stating) PRINCIPLE: Fungi and Pneumocystis organisms are oxidized using periodic acid and then impregnated with ammoniacal silver solution. The silver is then toned from brown to black with gold chloride. Any unreduced silver is removed with sodium thiosulfate, leaving organisms stained black against a green counterstained background. SPECIMENS: Formalin fixed, paraffin embedded tissue cut at 4-5 microns or 95% alcohol fixed smears or monolayer cell preparations. CONTROL: Any tissue known to contain a fungus or Pneumocystis organisms REAGENTS: 1. Periodic acid 1% Aqueous 2. Ammoniacal GMS solution (Silver) 3. Gold Chloride 0.5% Aqueous 4. Sodium Thiosulfate 5% Aqueous 5. Fast Green Working Solution All working solutions are purchased from PolyScientific Kit #K101. Individual components may be purchased separately as they are exhausted. PROCEDURE: 1. Deparaffinize and hydrate to water 2. Using a plastic Coplin jar with the lid resting upside down on top, microwave 50.ml of 1% Periodic Acid at for 30 seconds at power 5. 3. Place slides in the hot solution, covered, for 5 minutes. 4. Wash in 5 changes of DI Water. 5. Using a plastic Coplin jar with the lid resting upside down on top, microwave 40.ml of Ammoniacal Silver Solution at for 30 seconds on HIGH power. 6. Using a plastic disposable pipet, vigorously stir the solution for a 3 seconds. 7. Place the slides in the hot silver solution 8. Replace the lid upside down on top of the Coplin jar and microwave for an additional 20 seconds on power 5. 9. For fungus, let the slides stand for exactly 1 minute following the last microwave step. For pneumocystis, let the slides sit for exactly 2 minutes following the last microwave step/ 10. Wash in 5 changes of DI Water 11. With slides lying flat, dispense enough Gold Chloride to cover the slide for 1 minute. Rinse in DI Water. 12. With slides lying flat, dispense enough Sodium Thiosulfate solution to cover the slide for 2 minutes. Rinse in DI Water. 13. With slides lying flat, dispense enough Light Green to cover the slide for 1 minute. Rinse in DI Water. 14. Dehydrate through graded alcohols, clear in 2 changes of Xylene and mount with a compatible mounting media. NOTE: Microwaves differ in power output. This procedure is based on our current microwave. If the power changes or the microwave is changed, then the timing of this procedure may have to be adjusted and the stain revalidated. ? RESULTS Fungi ? Black Pneumocystis ? Black to blue black Mucin ? Pale Gray Background - Green REFERENCE: Kit Procedure Insert, Rapid GMS for Fungus and Pneumocystis Carinii, Cat# k101, Poly Scientific R&D Corporation, Bay Shore NY 11706 Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 ********************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From lpwenk <@t> sbcglobal.net Mon Jun 9 20:07:04 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jun 9 20:07:09 2014 Subject: [Histonet] Should I leave histology world In-Reply-To: References: Message-ID: <30861882E8214F799D69632F87236E64@HP2010> You didn't mention what your current cutting speed is, nor what speed they want you to be at. Ask your supervisor to set up goals for you to meet, with time frames. Each week, work on getting faster, not fast today, just faster each week. At the School where I taught, we had goals set up during the time the students were learning, and also time when they were doing their histology lab rotation. When they were learning in the School, they would microtome 1-3 times a week for the first 2 months, and nearly daily the last 2 months, about 2 hours a day (for total of 120+ hours microtoming before their histology rotation). The first couple of months, emphasis was on quality, and then it shifted to quantity (speed) and quality. Every 4-6 weeks, they were reassessed as to whether they were meeting quality and quantity (speed) goals. When they did their 1 month rotation through histology lab, they were full time in the lab, and microtomed about 2-3 hours a day (total about an additional 100 hours microtoming). They were expected to keep the quality up, and still improve their quantity (speed). When they graduated, no, they were not as fast as experienced techs, but they were close. At any time (school or rotations), if they didn't measure up to the goals, they knew they had to repeat the evaluation and/or rotation. If they still could not meet the goals, they would be let go from the program. So they had to pass the academic part (tests for what they know) as well as psychomotor (DOING the sectioning, staining, embedding, etc.), and the affective (showing up on time, getting along with people, willing to volunteer, etc.). This is the same as any histotech job. There are goals (quality and quantity (speed)) that everyone must be able to meet. You have been microtoming at your second job for 2 months, 8 hours a day, and 2-3 hours a day since April (up to 2 months) at your current job. That's about 400 hours of microtoming while at jobs. At this point, you should have quality down pat, and should be able to concentrate on speed. So have your supervisor set specific speed goals and dates, and then you use those time intervals to keep improving your speed. Have your supervisor watch you, and see where you are being slowed down by unnecessary movements. And if you are the only histotech responding to the data entry person, then explain it to your supervisor, and get that responsibility on a rotating basis amongst all of you. Peggy Wenk HTL (ASCP) SLS -----Original Message----- From: Alpha Histotech Sent: Tuesday, June 03, 2014 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Should I leave histology world Hi everyone, I wouldn't give too much detail information as the histology world is very small and everyone knows everyone. I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The first place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 places I won't mention and I currently still have a histology job. My problem is all the places I worked were factory style lab work and they all did derm work. In my career I really only embedded most of the time. I did occasional other stuff like special stains both by hand and using Dako Artisan and other things like cytology cytospin. But I never got to develop in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me and put me back to embed. My 2nd job put me to cut the last 2 months (full 8hrs) I was working there. My current job I have been cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I have been here now 1 yr, I was embedding most of the time before th cutting started). I was told by my director I need to speed up in cutting because corporate is asking why I am not increasing in speed. And if I don't speed up eventually then they will have to demote me to a lab aid and give me a pay cut. (where I work and the state I work in they have lab aids doing alot of stuff without being certified, it wasn't like that in the other state I am original from as you have to be state licensed and ascp) I sometimes laugh inside my head because before my director hired me I told him I don't have alot experience in cutting. Now everywhere I have gone...speed is the name of the game. They say they care about quality but in the end if you can't put up then you will be put out! So I am just thinking I should just get out of histology world all together. Every where I have worked unfortunately have management who believe quantity over quality. OR Do you guys think I need more time cutting to develop speed? Beforehand I did need a little learning curve to cut and I have gotten through that now. It's just the speed that is killing me. And I also see if anyone at my work detours me for any reason like for example data entry person from front desk ask for missing gross dictation, then that lost time is very hard to recover as I am not soooo fast to recover. I feel I may have to become very rude(not help) with everyone I work around in order to stay glued to my seat when I am cutting my blocks. One thing I want to say also...until this day I never been written up for quality issues and I never lost any tissue while embedding. Embedding I am fast as most histotech (1 block a min or most times 30-45 secs 1 block) with proper embedding techniques demonstrated (tissue on same plane, tissue embedded with proper orientation and follow any other necessary embedding instructions. ) I just feel I haven't done my time in cutting as I did in embedding to become a fast cutter. I don't know if its because of working in a derm lab that management won't wait too long for you to develop like maybe a hospital lab may do. I was also thinking to find another histo job but not mention any of my experience so expectation won't be so high and I can get more time to develop. All of this also causes alot of stress and anxiety as it gets hard to coop with. What do you guys think and how I should go about with this. I am also not limited to histology. I have expertise in 2 other major fields that I wont mention because I don't want to be identified. I am also in my late 20's. Thanks for reading my post and I await your opinions as some of you all are veterans in the field of histology. Thank you Alpha Histotech (ASCP HT) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Jun 10 07:48:09 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jun 10 07:48:28 2014 Subject: [Histonet] Leica special/h&e stainer In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FD267E4@EMBX-CHAM2.cdc.gov> References: <1402340783698.503@accuratediagnosticlabs.com> <3B2CD438E1628A41BD687E98B963B7811FD267E4@EMBX-CHAM2.cdc.gov> Message-ID: Same here - we love them both.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Monday, June 09, 2014 6:24 PM To: 'Barbara Tibbs'; 'Deloris Carter'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Leica special/h&e stainer We have one as well but the special stains function for us was just something we thought we might use...we did purchase the bucket inserts just in case. But it was incompatible with simultaneously ding H&E's so we bought a Dako Artisan just for specials and love it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Monday, June 09, 2014 3:07 PM To: Deloris Carter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica special/h&e stainer I have the Leica Multistainer. It was purchased before I got here. The only thing I don't like is that the programs have to be "compatible" or else the machine alarms when you load a special stain onto the machine when it already has an H&E going. There's a rather complicated way to get the programs to be compatible which was explained to me when I went for training. You have to use an excel spreadsheet and figure out what rinse is where during all the programs. Yes, it's something Leica's engineers have to work on. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deloris Carter Sent: Monday, June 09, 2014 5:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica special/h&e stainer Does anyone have any feedback on the Leica Multistainer? It stains H&E as well as special stains. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From CDavis <@t> che-east.org Tue Jun 10 07:47:07 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Tue Jun 10 07:49:58 2014 Subject: [Histonet] H & E Stainers In-Reply-To: References: Message-ID: Hi Guys, Please we are in the process of buying a new H & E Stainers and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 We have the TissueTek Prisma minus the coverslipper connection and highly reccomend it. (the coverslipper connection is on our budget request) Cassandra Davis CDavis@che-east.org 302-575-8095 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Monday, June 09, 2014 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: DAB away alternative (Dario Ricca) 2. G.I. biopsies and special stains (Kathy.Machado@LPNT.net) 3. Re: G.I. biopsies and special stains (Michael Ann Jones) 4. RE: G.I. biopsies and special stains (Hannen, Valerie) 5. Re: G.I. biopsies and special stains (Sue) 6. RE: G.I. biopsies and special stains (Tom McNemar) 7. RE: G.I. biopsies and special stains (Boyd, Debbie M) 8. RE: G.I. biopsies and special stains (Mike Pence) 9. Re: RE: G.I. biopsies and special stains (Victoria Baker) 10. Re: G.I. biopsies and special stains (Michael Ann Jones) 11. Documenting special stain control blocks (Histology) 12. Special Stainers (Adesupo, Adesuyi (Banjo)) 13. FW:H & E Stainers (Adesupo, Adesuyi (Banjo)) 14. Re: Special Stainers (Rene J Buesa) 15. RE: Special Stainers (Barbara Tibbs) 16. RE: Special Stainers (Cooper, Brian) ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Jun 2014 12:02:16 +0200 From: Dario Ricca Subject: Re: [Histonet] DAB away alternative To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi Thomas, I've see that Dako's DAB Away is composed of 1% Oxalic acid in water instead other DAB Aways (like the one you are using) are composed of sulfuric acid <10% in water. Dario 2014-06-06 17:53 GMT+02:00 Thomas Pier : > Hello Histonet, > We have been using Lab Vision's DAB away to clean our Autostainer 360. > However it seems a little wasteful to buy something that we can't use up > before it expires. Do any of you have any exerpience with lower cost > alternatives? > > Thanks, > Tom > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 2 Date: Mon, 9 Jun 2014 08:14:42 -0500 From: Subject: [Histonet] G.I. biopsies and special stains To: Message-ID: Content-Type: text/plain; charset="us-ascii" I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 ------------------------------ Message: 3 Date: Mon, 9 Jun 2014 13:37:42 +0000 From: Michael Ann Jones Subject: Re: [Histonet] G.I. biopsies and special stains To: "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We order HP by IHC automatically on all stomach and GE juction specimens. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/9/14 7:14 AM, "Kathy.Machado@LPNT.net" wrote: >I was wondering if other labs are "automatically" ordering special stains >and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait >until H&E is screened? Thanks! > >Danville Regional Medical Center >Danville, VA >Kathy.Machado@lpnt.net >434-799-3868 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 9 Jun 2014 09:42:23 -0400 From: "Hannen, Valerie" Subject: [Histonet] RE: G.I. biopsies and special stains To: "'Kathy.Machado@LPNT.net'" , "histonet@lists.utsouthwestern.edu" Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB793@isexstore03> Content-Type: text/plain; charset="us-ascii" We automatically order CD3 and ALC BL/PAS on small bowel, ALC/BL PAS on Barrett's esophagus. H.P. on stomach. Per our Pathologist's order. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Machado@LPNT.net Sent: Monday, June 09, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 5 Date: Mon, 9 Jun 2014 10:01:12 -0400 From: Sue Subject: Re: [Histonet] G.I. biopsies and special stains To: "" Cc: "" Message-ID: <2450D699-E1C5-45D6-8B4F-BC8C7FA41877@comcast.net> Content-Type: text/plain; charset=us-ascii We auto do hp on gastric. Anything else is ordered by path. u need to be careful with ins. if neg the may not re-emburse. Sent from my iPhone > On Jun 9, 2014, at 9:14 AM, wrote: > > I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! > > Danville Regional Medical Center > Danville, VA > Kathy.Machado@lpnt.net > 434-799-3868 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 9 Jun 2014 10:05:01 -0400 From: Tom McNemar Subject: [Histonet] RE: G.I. biopsies and special stains To: "'Kathy.Machado@LPNT.net'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" H. Pylori by IHC on all stomach biopsies and Alcian Blue on all esophageal biopsies done automatically. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Machado@LPNT.net Sent: Monday, June 09, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 7 Date: Mon, 9 Jun 2014 14:05:05 +0000 From: "Boyd, Debbie M" Subject: [Histonet] RE: G.I. biopsies and special stains To: "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9541875@TN001WEXMBX12.US.chs.net> Content-Type: text/plain; charset="us-ascii" We have just discontinued this practice after Jane Pine Wood (renowned Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E stain is not reasonable and necessary". According to the pathologist billing company ,there is in an article they have privy to with wording which threatens "additional action" taken for providers whose ordering of special stains exceeds the 20th percentile of Gastric Pathology cases. For this reason we are now retro ordering special stains on gastric biopsies. We are also looking at IHC necessity. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] Sent: Monday, June 09, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 8 Date: Mon, 9 Jun 2014 14:11:05 +0000 From: Mike Pence Subject: [Histonet] RE: G.I. biopsies and special stains To: "'Boyd, Debbie M'" , "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" You gotta love Obamacare! Where "Legal meets Medical". -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Monday, June 09, 2014 9:05 AM To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: G.I. biopsies and special stains We have just discontinued this practice after Jane Pine Wood (renowned Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E stain is not reasonable and necessary". According to the pathologist billing company ,there is in an article they have privy to with wording which threatens "additional action" taken for providers whose ordering of special stains exceeds the 20th percentile of Gastric Pathology cases. For this reason we are now retro ordering special stains on gastric biopsies. We are also looking at IHC necessity. Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH 804-765-5050 l FAX 804-765-8852 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] Sent: Monday, June 09, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] G.I. biopsies and special stains I was wondering if other labs are "automatically" ordering special stains and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait until H&E is screened? Thanks! Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3868 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 9 Jun 2014 10:14:47 -0400 From: Victoria Baker Subject: Re: [Histonet] RE: G.I. biopsies and special stains To: Mike Pence Cc: "Boyd, Debbie M" , histonet netserver , "Kathy.Machado@LPNT.net" Message-ID: Content-Type: text/plain; charset=UTF-8 AKA - managed care. Emphasis on managed. On Jun 9, 2014 10:11 AM, "Mike Pence" wrote: > You gotta love Obamacare! Where "Legal meets Medical". > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M > Sent: Monday, June 09, 2014 9:05 AM > To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: G.I. biopsies and special stains > > We have just discontinued this practice after Jane Pine Wood (renowned > Healthcare Attorney and Medical Director for Palmetto Medicare Part B > coving NC, SC, VA, and WV) stated "ordering special stains prior to review > of the H&E stain is not reasonable and necessary". According to the > pathologist billing company ,there is in an article they have privy to with > wording which threatens "additional action" taken for providers whose > ordering of special stains exceeds the 20th percentile of Gastric Pathology > cases. For this reason we are now retro ordering special stains on gastric > biopsies. We are also looking at IHC necessity. > > Debbie M. Boyd HT (ASCP) l Chief Histologist l Southside Regional Medical > Center l 200 Medical Park Blvd. l Petersburg, Va. 23805 l PH > 804-765-5050 l FAX 804-765-8852 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of > Kathy.Machado@LPNT.net [Kathy.Machado@LPNT.net] > Sent: Monday, June 09, 2014 9:14 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] G.I. biopsies and special stains > > I was wondering if other labs are "automatically" ordering special stains > and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait > until H&E is screened? Thanks! > > Danville Regional Medical Center > Danville, VA > Kathy.Machado@lpnt.net > 434-799-3868 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -------------------------------------------------------------------------- > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Mon, 9 Jun 2014 14:33:05 +0000 From: Michael Ann Jones Subject: Re: [Histonet] G.I. biopsies and special stains To: "Rathborne, Toni" , "Kathy.Machado@LPNT.net" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We bill all of the stomach HP, but we do not bill all of the GE specimens. Only those deemed necessary (if we even do them - some pathologists do not want them, some do) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/9/14 8:30 AM, "Rathborne, Toni" wrote: >We wait for H&E. >For the labs that order automatically, do you bill all of them, or just >the ones determined to be necessary? > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael >Ann Jones >Sent: Monday, June 09, 2014 9:38 AM >To: Kathy.Machado@LPNT.net; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] G.I. biopsies and special stains > >We order HP by IHC automatically on all stomach and GE juction specimens. >Michael Ann Jones, HT (ASCP) >Histology Manager >Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 6/9/14 7:14 AM, "Kathy.Machado@LPNT.net" >wrote: > >>I was wondering if other labs are "automatically" ordering special >>stains and/or IHC on stomach biopsies and esophageal biopsies? Or do >>you wait until H&E is screened? Thanks! >> >>Danville Regional Medical Center >>Danville, VA >>Kathy.Machado@lpnt.net >>434-799-3868 >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 11 Date: Mon, 9 Jun 2014 14:33:01 +0000 From: Histology Subject: [Histonet] Documenting special stain control blocks To: "histonet@lists.utsouthwestern.edu" Message-ID: <566E7795BC94204D9A21539DE282AED709A370@DC01.dp.local> Content-Type: text/plain; charset="us-ascii" Hi All, Just wondering how everyone is handling the new checklist question ANP. 21450? How are you documenting results of verified special stain tissue blocks? Thanks for any help, ------------------------------ Message: 12 Date: Mon, 9 Jun 2014 10:03:32 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] Special Stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82215D@MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 13 Date: Mon, 9 Jun 2014 10:27:35 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] FW:H & E Stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B82215E@MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" ________________________________ From: Adesupo, Adesuyi (Banjo) Sent: Monday, June 09, 2014 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: Special Stainers Hi Guys, Please we are in the process of buying a new H & E Stainers and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 14 Date: Mon, 9 Jun 2014 08:53:10 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Special Stainers To: "Adesupo, Adesuyi \(Banjo\)" , "histonet@lists.utsouthwestern.edu" Message-ID: <1402329190.688.YahooMailNeo@web120403.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, ? ? ? ? ? ? Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1.? Symphony by ventana 1.? TissueTek? Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, ? Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS ? Histology Supervisor ? Norman Regional Health System, ? Norman, OK 73071. ? Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Mon, 9 Jun 2014 16:00:56 +0000 From: Barbara Tibbs Subject: RE: [Histonet] Special Stainers To: "Adesupo, Adesuyi (Banjo)" , "histonet@lists.utsouthwestern.edu" , "Rene J Buesa" Message-ID: <1402329636943.16774@accuratediagnosticlabs.com> Content-Type: text/plain; charset="iso-8859-1" You cannot go wrong with the Prisma. I used that in another lab and it was wonderful. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Monday, June 09, 2014 2:53 PM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special Stainers Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 9 Jun 2014 16:15:53 +0000 From: "Cooper, Brian" Subject: RE: [Histonet] Special Stainers To: "Adesupo, Adesuyi (Banjo)" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=iso-8859-1 The Ventana Symphony is undoubtedly the easiest stainer to use and maintain. You will never have an employee put an incorrect reagent into a container, and direct to drain waste makes it an appealing option. The H&E's are absolutely beautiful! Symphony is finicky about certain things though--the type of alcohol you use, the humidity levels in your lab, and the unit just doesn't like charged slides. Bubbles in coverslips and slides sticking together when filing may or may not be an issue. That being said, I've used Prisma's predecessors (the DRS 2000) and, no matter what, those things NEVER failed. We had three of them that ran 50-70 runs per day for around 10 years, and I can count on one hand the number of times they weren't functional. These are open container stainers though, and do require diligent maintenance and reagent changes/rotations. If you can handle that, I'd say go with the Prisma. Thanks, Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Monday, June 09, 2014 9:01 AM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu; Rene J Buesa Subject: RE: [Histonet] Special Stainers You cannot go wrong with the Prisma. I used that in another lab and it was wonderful. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Monday, June 09, 2014 2:53 PM To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Special Stainers Prisma every day Ren? J. On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" wrote: Hi Guys, Please we are in the process of buying a new Special Stainer and will appreciate it, if you guys could share your experience(s) with me. The two stainers we are still looking at are stated below; 1. Symphony by ventana 1. TissueTek Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper Please let me know whether you guys have had any experience with either of the equipments or both. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 12 ***************************************** Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From JBabus <@t> som.umaryland.edu Tue Jun 10 07:53:56 2014 From: JBabus <@t> som.umaryland.edu (Babus, Janice) Date: Tue Jun 10 07:54:01 2014 Subject: [Histonet] please remove me Message-ID: <657B8ABE3D532640BBA2384E115CEF0C7BB3CA@ISSOMEXCHMB04.som.umaryland.edu> From Joyce.Weems <@t> emoryhealthcare.org Tue Jun 10 08:00:09 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jun 10 08:00:18 2014 Subject: [Histonet] RE: please remove me In-Reply-To: <657B8ABE3D532640BBA2384E115CEF0C7BB3CA@ISSOMEXCHMB04.som.umaryland.edu> References: <657B8ABE3D532640BBA2384E115CEF0C7BB3CA@ISSOMEXCHMB04.som.umaryland.edu> Message-ID: Hi Janice, You must do that yourself at Histonet@lists.utsouthwestern.edu Thanks! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Babus, Janice Sent: Tuesday, June 10, 2014 8:54 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] please remove me _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From histomngr <@t> yahoo.com Tue Jun 10 09:17:57 2014 From: histomngr <@t> yahoo.com (George) Date: Tue Jun 10 09:18:02 2014 Subject: [Histonet] Remove from histonet Message-ID: <51E53E47-45B7-4C2D-B62D-A869850F1A0E@yahoo.com> Sent from my iPhone From Joyce.Weems <@t> emoryhealthcare.org Tue Jun 10 09:51:24 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jun 10 09:52:04 2014 Subject: [Histonet] Remove from histonet In-Reply-To: <51E53E47-45B7-4C2D-B62D-A869850F1A0E@yahoo.com> References: <51E53E47-45B7-4C2D-B62D-A869850F1A0E@yahoo.com> Message-ID: Hi George, You must do that yourself at Histonet@lists.utsouthwestern.edu Thanks! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of George Sent: Tuesday, June 10, 2014 10:18 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Remove from histonet Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From cllamas <@t> tulane.edu Tue Jun 10 11:30:55 2014 From: cllamas <@t> tulane.edu (Llamas, Claire B) Date: Tue Jun 10 11:31:19 2014 Subject: [Histonet] remove from listserv Message-ID: hi, please remove me from the listserv. thank you, claire. llamas Claire Llamas Tulane University - Center for Stem Cell Research and Regenerative Medicine Health Sciences Center J. Bennett Johnston Building, Rm. 671 1324 Tulane Avenue, SL-99 New Orleans, LA 70112-2699 Phone: 504-988-7071 FAX: 504-988-7710 From Michael.LaFriniere <@t> ccplab.com Tue Jun 10 11:41:07 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Tue Jun 10 11:41:23 2014 Subject: [Histonet] RE: GMS better than PAS for Fungi In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157E00EB10B@xmdb04.nch.kids> References: <6D6BD1DE8A5571489398B392A38A7157E00EB10B@xmdb04.nch.kids> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29673F4E@AHCMSASEXCH02.my.ahc.local> I must disagree Tony, with my experience,using Periodic acid instead of chromic acid in the GMS stain I have no problem demonstrating Pseudo fungi nor strong results demonstrating Pneumocystis, think you may be confusing stains, this is not a (PSAM)that I am aware of. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Wednesday, June 04, 2014 7:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS better than PAS for Fungi Thought that the subject title should be changed. (References available on request) No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience is that they are GMS (using chromic acid) negative. It is possible that our pathologists aren't aware that the "GMS" that their lab does might be using Periodic acid instead of Chromic acid. Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of chromic acid. The literature often does not report the exact GMS used which makes clear understanding of the histochemical results difficult. Pneumocystis will not be easy to see unless chromic acid is used (the mucin stains strongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 5 June 2014 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acid Clean Glassware Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. Acid Cleaned glassware (Abbott, Tanya) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Jun 10 11:43:47 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jun 10 11:43:53 2014 Subject: [Histonet] RE: remove from listserv In-Reply-To: References: Message-ID: Hi Claire, You must do that yourself at Histonet@lists.utsouthwestern.edu Thanks! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Llamas, Claire B Sent: Tuesday, June 10, 2014 12:31 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] remove from listserv hi, please remove me from the listserv. thank you, claire. llamas Claire Llamas Tulane University - Center for Stem Cell Research and Regenerative Medicine Health Sciences Center J. Bennett Johnston Building, Rm. 671 1324 Tulane Avenue, SL-99 New Orleans, LA 70112-2699 Phone: 504-988-7071 FAX: 504-988-7710 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From GaleL <@t> unionhospital.org Tue Jun 10 11:58:58 2014 From: GaleL <@t> unionhospital.org (Gale Limron) Date: Tue Jun 10 11:59:03 2014 Subject: [Histonet] stains Message-ID: Hello, Well, our ancient incubator finally died. We didn't use it often except for a couple of special stains that we do manually. Does anyone have a microwave procedure for PAS with Diastase or Fontana-Masson that they would share? Thanks, Gale Gale Limron HT, CT (ASCP) Histology Supervisor Union Hospital 659 Boulevard Dover, Ohio 44622 330-343-3311 ext 2562 This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From pscott <@t> scigene.com Tue Jun 10 12:42:37 2014 From: pscott <@t> scigene.com (Paul Scott) Date: Tue Jun 10 12:42:42 2014 Subject: [Histonet] Re: Request for Non-fluorescent Sealant Message-ID: <00ca01cf84d3$5ef4a150$1cdde3f0$@com> Hi John, My company SciGene has a product called CytoBond Removable Coverslip Sealant that was designed for use in FISH as replacement to rubber cement for temporarily sealing of the coverslips during probe hybridization. Customers tell us how much easier it is to remove as opposed to rubber cement. If you send me your details I can get a free sample out to you. It is usually completely removed (with no remainder left behind) during imaging so I cannot provide feedback as to its autoflouresence over a spectral range. Paul Scott Technical Sales Representative SciGene 470 Lakeside Drive, Ste F, Sunnyvale, CA 94085-4720 408-733-7337 x 305 pscott@scigene.com Automating FISH and CMA Workflows www.SciGene.com Hello, I am wondering if anyone would know of a sealant that is non-fluorescent (or doesn't leak). I am using a biorad duo chamber slide which has two slots for each chamber, I need to measure the fluorescence of some sample but Rubber Cement and the nail polish I have tried have both had some interfering fluorescence. Any help is appreciated! -John From bfoster <@t> mme1.com Tue Jun 10 14:16:19 2014 From: bfoster <@t> mme1.com (Barbara Foster) Date: Tue Jun 10 14:16:32 2014 Subject: [Histonet] =?iso-8859-1?q?WEBINAR_Reminder=3A_How_Image_Integrit?= =?iso-8859-1?q?y_Impacts_Your_Ability_to=0D=0A__Publish=85_or_Perish=2E?= Message-ID: <201406101916.s5AJGNWr018592@atl4mhob06.myregisteredsite.com> Dear Listers, Just a reminder about tomorrow's webinar on image integrity. It will discuss some interesting stats re: reported cases of scientific misconduct based on published images. http://scientific.datacolor.com/free-new-chromacal-webinar/ Wednesday, June 11, 2014, 1:00PM EDT Retractions of papers from publication have increased tenfold between 2005 and 2011. Each incident affects the reputation of scientists, academic institutions, and public perception, which can lead to reduced funding from NIH and NSF. The role of images in publication has largely been a subtopic in Publication Ethics, yet reported cases of scientific misconduct in which images have been involved ballooned to 80% from 1989 to 2008. Images require a series of specific steps to be taken in order to protect, calibrate and create publication-ready images in research and reports. Guest presenter, Jerry Sedgewick, of Imaging and Analysis, LLC will discuss these issues in detail. For details and registration, visit http://scientific.datacolor.com/free-new-chromacal-webinar/ Best regards, Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education* www.MicroscopyEducation.com *A subsidiary of The Microscopy & Imaging Place, Inc. 7101 Royal Glen Trail, Suite A McKinney, TX 75070 P: 972-924-5310 F: 214-592-0277 MME is currently scheduling courses for now and through Fall 2014. Call us today for a free training evaluation. From tony.henwood <@t> health.nsw.gov.au Tue Jun 10 19:49:42 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Jun 10 19:50:06 2014 Subject: [Histonet] RE: GMS better than PAS for Fungi In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D29673F4E@AHCMSASEXCH02.my.ahc.local> References: <6D6BD1DE8A5571489398B392A38A7157E00EB10B@xmdb04.nch.kids> <4A2A16B9707CE04E9CB6C82DC18C1D29673F4E@AHCMSASEXCH02.my.ahc.local> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00EC233@xmdb04.nch.kids> Hi Michael, The PASM uses periodic acid, followed by the methenamine silver reaction. This is usually for basement membranes, though some methods, including mine, uses a thiosemicarbazide step before the silver. GMS uses Chromic acid followed by the methenamine silver reaction. The idea of a GMS is not to demonstrate pseudo-fungi. These are usually PAS positive. We would rather not have patients treated with anti-fungals if they do not actually have a fungus infection. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Michael LaFriniere [mailto:Michael.LaFriniere@ccplab.com] Sent: Wednesday, 11 June 2014 2:41 AM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: RE: GMS better than PAS for Fungi I must disagree Tony, with my experience,using Periodic acid instead of chromic acid in the GMS stain I have no problem demonstrating Pseudo fungi nor strong results demonstrating Pneumocystis, think you may be confusing stains, this is not a (PSAM)that I am aware of. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Wednesday, June 04, 2014 7:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS better than PAS for Fungi Thought that the subject title should be changed. (References available on request) No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience is that they are GMS (using chromic acid) negative. It is possible that our pathologists aren't aware that the "GMS" that their lab does might be using Periodic acid instead of Chromic acid. Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of chromic acid. The literature often does not report the exact GMS used which makes clear understanding of the histochemical results difficult. Pneumocystis will not be easy to see unless chromic acid is used (the mucin stains strongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 5 June 2014 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acid Clean Glassware Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. Acid Cleaned glassware (Abbott, Tanya) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Carolyn.Barnes2 <@t> va.gov Wed Jun 11 06:42:47 2014 From: Carolyn.Barnes2 <@t> va.gov (Barnes, Carolyn K. RICVAMC) Date: Wed Jun 11 06:43:37 2014 Subject: [Histonet] Storage of 10% NB formalin Message-ID: My lab is having a discussion about the storage location of our prefilled NB 10% formalin in a stock room. These containers haven't been used or opened. Does anyone know what the current recommendations are for the safe storage of NB 10% formalin? I seem to recall something about an allowed total volume of formalin in relationship with the total area of a given space. Carolyn K. Barnes Carolyn.Barnes2@va.gov From LBUSTAMANTE <@t> cvm.tamu.edu Wed Jun 11 08:56:21 2014 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Wed Jun 11 08:56:28 2014 Subject: [Histonet] Microwave Processor Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9012EF74933@CVMMB02.cvm.tamu.edu> I am looking to buy a NEW Microwave tissue processor. Could you please give me your pro and con for instruments available in your lab? Price is a big issue, if anyone knows about a cheap but very reliable I would like to hear about it PLEASE! Thank you very much. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) CTDDA Histology Lab Supervisor From Bruce_Palmatier <@t> vwr.com Wed Jun 11 09:01:03 2014 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Wed Jun 11 09:01:28 2014 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 06/13/2014) Message-ID: I am out of the office until 06/13/2014. I will be out of the office from June 10 through June 13. I will have limited access to email but will be responding as possible in the evening. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 127, Issue 13" sent on 6/11/2014 7:00:09 AM. This is the only notification you will receive while this person is away. From rjbuesa <@t> yahoo.com Wed Jun 11 09:02:46 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 11 09:05:52 2014 Subject: [Histonet] Microwave Processor In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC9012EF74933@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9012EF74933@CVMMB02.cvm.tamu.edu> Message-ID: <1402495366.80390.YahooMailNeo@web120405.mail.ne1.yahoo.com> Look at the options presented by Milestone Ren? J.? On Wednesday, June 11, 2014 9:56 AM, "Bustamante, Lin" wrote: I am looking to buy a NEW Microwave tissue processor. Could you please give me your pro and con for instruments available in your lab? Price is a big issue, if anyone knows about a cheap but very reliable I would like to hear about it PLEASE! Thank you very much. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) CTDDA Histology Lab Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mkent <@t> dermpathlab.com Wed Jun 11 10:30:13 2014 From: mkent <@t> dermpathlab.com (Michael Kent) Date: Wed Jun 11 10:30:22 2014 Subject: [Histonet] Re: Request for Non-fluorescent Sealant In-Reply-To: <00ca01cf84d3$5ef4a150$1cdde3f0$@com> References: <00ca01cf84d3$5ef4a150$1cdde3f0$@com> Message-ID: <0d21e0f7a85b2c62d67d1524b95c95d1@mail.gmail.com> John, I was recommended and have used black nail polish for FISH applications without background. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Scott Sent: Tuesday, June 10, 2014 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Request for Non-fluorescent Sealant Hi John, My company SciGene has a product called CytoBond Removable Coverslip Sealant that was designed for use in FISH as replacement to rubber cement for temporarily sealing of the coverslips during probe hybridization. Customers tell us how much easier it is to remove as opposed to rubber cement. If you send me your details I can get a free sample out to you. It is usually completely removed (with no remainder left behind) during imaging so I cannot provide feedback as to its autoflouresence over a spectral range. Paul Scott Technical Sales Representative SciGene 470 Lakeside Drive, Ste F, Sunnyvale, CA 94085-4720 408-733-7337 x 305 pscott@scigene.com Automating FISH and CMA Workflows www.SciGene.com Hello, I am wondering if anyone would know of a sealant that is non-fluorescent (or doesn't leak). I am using a biorad duo chamber slide which has two slots for each chamber, I need to measure the fluorescence of some sample but Rubber Cement and the nail polish I have tried have both had some interfering fluorescence. Any help is appreciated! -John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Wed Jun 11 11:24:53 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jun 11 11:24:58 2014 Subject: [Histonet] Aperio slide scanner Message-ID: My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive003@umn.edu From liz <@t> premierlab.com Wed Jun 11 11:46:23 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 11 11:46:29 2014 Subject: [Histonet] Aperio slide scanner In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05D29@SBS2K8.premierlab.local> Jan They are not that difficult to operate but you do need person with some histology training scanning and QC'ing the slides and images. The need to understand initially if the section is good enough to scan, we QC our slides prior to placing them on the scanner, ones that have sectioning artifacts may not scan that well. Section placement on the slide is important, you can't have anything to close to the edge of a slide it will not scan well. The person responsible for scanning makes sure that the slide is clean and does not have any excess mounting media prior to placing on the scanner, excess mounting media and dirt may cause the scan to be out of focus, and then once the slides are scanned the scans need to be QC'd to make sure that they are good enough for the pathologist or whatever you are utilizing them for. Quality and consistency in histology preparation is key for good scanning results. These scanners scan in primarily one focal plane, meaning the pathologist loses the ability to fine focus as they would on a microscope. They are able to fine focus through poorer quality sections or uneven sections, scanned images do not have a fine focus unless you scan them at multiple focal planes or z-stack. I have seen some cytology images that have been scanned in a way that you have a fine focus slider but its not common for routine histology preps. We take some time upfront to adjust area of interest and check focal points prior to scanning of the slides. We find this works better than just going with the load and go method. We review the snapshots prior to scanning. I'm not sure what scanner you are getting or what version of the image capture software you will be using we have an Aperio ScanScope XT it has a 120 slide capacity. On occasion a particular slide may not scan well, that?s why we like to review the snapshots. For instance we were working on some amniotic membrane constructs these are a single cell layer thick so they are very thin and sometimes the computer does not pick the sample up as tissue because it so thin and can be lightly stained, so we need to place all of the focal points on the slides. You may not have samples like this but you might. Good Luck - feel free to contact me if you need any help once you get the scanner in house. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, June 11, 2014 10:25 AM To: histonet Subject: [Histonet] Aperio slide scanner My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Wed Jun 11 12:31:31 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Jun 11 12:31:45 2014 Subject: [Histonet] Aperio slide scanner In-Reply-To: References: Message-ID: Jan, We like ours a lot. And I agree with all that Liz mentioned. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, June 11, 2014 9:25 AM To: histonet Subject: [Histonet] Aperio slide scanner My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Wed Jun 11 12:38:36 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Jun 11 12:38:41 2014 Subject: [Histonet] Aperio slide scanner In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E05D29@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79E05D29@SBS2K8.premierlab.local> Message-ID: I will echo Liz's comments. I occasionally scan slides with an older Scanscope XT. It is worth the effort to clean and QC slides for artifacts as Liz points out as well as placing the section in the middle of the slide. Actual scanning is quite easy, each initial 'snapshot' is reviewed, AOI is resized to boundaries of the tissue and focus points are checked and verified to be overlaying tissue (not white space). I find that adding more focus points across the section greatly increased the quality of the image and rarely have I found any areas out of focus. Once the snapshot reviews are completed simply hit the 'One Touch' icon and batch scanning begins. I really like the ImageScope viewing program which lets one view the section and multiple magnifications, capture images, etc.. I have another system for IHC quantification, but other users are using the Aperio software to do that if that is part of your plan Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, June 11, 2014 12:46 PM To: Jan Shivers; histonet Subject: RE: [Histonet] Aperio slide scanner Jan They are not that difficult to operate but you do need person with some histology training scanning and QC'ing the slides and images. The need to understand initially if the section is good enough to scan, we QC our slides prior to placing them on the scanner, ones that have sectioning artifacts may not scan that well. Section placement on the slide is important, you can't have anything to close to the edge of a slide it will not scan well. The person responsible for scanning makes sure that the slide is clean and does not have any excess mounting media prior to placing on the scanner, excess mounting media and dirt may cause the scan to be out of focus, and then once the slides are scanned the scans need to be QC'd to make sure that they are good enough for the pathologist or whatever you are utilizing them for. Quality and consistency in histology preparation is key for good scanning results. These scanners scan in primarily one focal plane, meaning the pathologist loses the ability to fine focus as they would on a microscope. They are able to fine focus through poorer quality sections or uneven sections, scanned images do not have a fine focus unless you scan them at multiple focal planes or z-stack. I have seen some cytology images that have been scanned in a way that you have a fine focus slider but its not common for routine histology preps. We take some time upfront to adjust area of interest and check focal points prior to scanning of the slides. We find this works better than just going with the load and go method. We review the snapshots prior to scanning. I'm not sure what scanner you are getting or what version of the image capture software you will be using we have an Aperio ScanScope XT it has a 120 slide capacity. On occasion a particular slide may not scan well, that?s why we like to review the snapshots. For instance we were working on some amniotic membrane constructs these are a single cell layer thick so they are very thin and sometimes the computer does not pick the sample up as tissue because it so thin and can be lightly stained, so we need to place all of the focal points on the slides. You may not have samples like this but you might. Good Luck - feel free to contact me if you need any help once you get the scanner in house. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, June 11, 2014 10:25 AM To: histonet Subject: [Histonet] Aperio slide scanner My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From liz <@t> premierlab.com Wed Jun 11 12:45:07 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 11 12:45:13 2014 Subject: [Histonet] Aperio slide scanner In-Reply-To: References: <14E2C6176416974295479C64A11CB9AE019C79E05D29@SBS2K8.premierlab.local> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05D2D@SBS2K8.premierlab.local> We load and scan the same way Brett does, it works quite well and we rarely have to rescan anything. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Connolly, Brett M [mailto:brett_connolly@merck.com] Sent: Wednesday, June 11, 2014 11:39 AM To: Elizabeth Chlipala; Jan Shivers; histonet Subject: RE: [Histonet] Aperio slide scanner I will echo Liz's comments. I occasionally scan slides with an older Scanscope XT. It is worth the effort to clean and QC slides for artifacts as Liz points out as well as placing the section in the middle of the slide. Actual scanning is quite easy, each initial 'snapshot' is reviewed, AOI is resized to boundaries of the tissue and focus points are checked and verified to be overlaying tissue (not white space). I find that adding more focus points across the section greatly increased the quality of the image and rarely have I found any areas out of focus. Once the snapshot reviews are completed simply hit the 'One Touch' icon and batch scanning begins. I really like the ImageScope viewing program which lets one view the section and multiple magnifications, capture images, etc.. I have another system for IHC quantification, but other users are using the Aperio software to do that if that is part of your plan Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, June 11, 2014 12:46 PM To: Jan Shivers; histonet Subject: RE: [Histonet] Aperio slide scanner Jan They are not that difficult to operate but you do need person with some histology training scanning and QC'ing the slides and images. The need to understand initially if the section is good enough to scan, we QC our slides prior to placing them on the scanner, ones that have sectioning artifacts may not scan that well. Section placement on the slide is important, you can't have anything to close to the edge of a slide it will not scan well. The person responsible for scanning makes sure that the slide is clean and does not have any excess mounting media prior to placing on the scanner, excess mounting media and dirt may cause the scan to be out of focus, and then once the slides are scanned the scans need to be QC'd to make sure that they are good enough for the pathologist or whatever you are utilizing them for. Quality and consistency in histology preparation is key for good scanning results. These scanners scan in primarily one focal plane, meaning the pathologist loses the ability to fine focus as they would on a microscope. They are able to fine focus through poorer quality sections or uneven sections, scanned images do not have a fine focus unless you scan them at multiple focal planes or z-stack. I have seen some cytology images that have been scanned in a way that you have a fine focus slider but its not common for routine histology preps. We take some time upfront to adjust area of interest and check focal points prior to scanning of the slides. We find this works better than just going with the load and go method. We review the snapshots prior to scanning. I'm not sure what scanner you are getting or what version of the image capture software you will be using we have an Aperio ScanScope XT it has a 120 slide capacity. On occasion a particular slide may not scan well, that?s why we like to review the snapshots. For instance we were working on some amniotic membrane constructs these are a single cell layer thick so they are very thin and sometimes the computer does not pick the sample up as tissue because it so thin and can be lightly stained, so we need to place all of the focal points on the slides. You may not have samples like this but you might. Good Luck - feel free to contact me if you need any help once you get the scanner in house. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, June 11, 2014 10:25 AM To: histonet Subject: [Histonet] Aperio slide scanner My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From mgg6 <@t> mit.edu Wed Jun 11 13:57:18 2014 From: mgg6 <@t> mit.edu (Michael Giacomelli) Date: Wed Jun 11 13:57:26 2014 Subject: [Histonet] non-fluorescent tissue marking dyes Message-ID: I am working on a pathology study involving two photon microscopy of pathology samples. For our study we need to be able to mark the surface aspects of our tissue with dye prior to histology processing and multiphoton imaging. Unfortunately, most of the standard tissue marking dyes we use have extraordinarily strong fluorescence in the blue/green when excited in the near UV (2 photon wavelength). This strong fluorescence then triggers the overcurrent protection on our detectors if we stray onto the surface aspect. Are there any tissue marking dyes that are known to survive histology processing and also generate little or no blue/green fluorescence? I'm not concerned about yellow/red fluorescence (its filtered out), or attenuation without fluorescence as we don't generally image the ink directly, it just sometimes gets in the way. I've ordered a few dyes for testing based on their composition, but I'm essentially just guessing because I haven't been able to find much information. I suppose not many people need to fluorescent image inked specimens. Thanks, Mike From delsuec <@t> gmail.com Wed Jun 11 13:59:12 2014 From: delsuec <@t> gmail.com (Deloris Carter) Date: Wed Jun 11 13:59:15 2014 Subject: [Histonet] Dako Message-ID: Any feedback out there on the Dako Omnis? Deloris Carter HT(ASCP) From rjr6 <@t> psu.edu Thu Jun 12 09:42:07 2014 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Thu Jun 12 09:42:11 2014 Subject: [Histonet] rolling sections Message-ID: I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University From hfedor <@t> jhmi.edu Thu Jun 12 09:54:45 2014 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Thu Jun 12 09:54:55 2014 Subject: [Histonet] RE: rolling sections In-Reply-To: References: Message-ID: Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Thu Jun 12 09:58:03 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Jun 12 09:58:10 2014 Subject: [Histonet] RE: rolling sections In-Reply-To: References: Message-ID: We do the same thing on our lab. It isn't necessary for them to roll....we just catch them and fold them up and put them in the tube. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, June 12, 2014 10:55 AM To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Jun 12 09:58:46 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jun 12 09:58:51 2014 Subject: [Histonet] RE: rolling sections In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0D02D6@evcspmbx2.ads.northwestern.edu> True. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, June 12, 2014 9:58 AM To: Helen Fedor; 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections We do the same thing on our lab. It isn't necessary for them to roll....we just catch them and fold them up and put them in the tube. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, June 12, 2014 10:55 AM To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Thu Jun 12 09:59:37 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Jun 12 09:59:44 2014 Subject: [Histonet] RE: rolling sections In-Reply-To: Message-ID: <321366197.150096.1402585177602.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> That is how we do it also.? Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll.? If they are doing DNA or RNAase ou may need to wear glove to prevent any contamination of the blocks.? Pam Marcum UAMS ----- Original Message ----- From: "Helen Fedor" To: "Roberta Horner" , "Histonet (histonet@lists.utsouthwestern.edu)" Sent: Thursday, June 12, 2014 9:54:45 AM Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. ?They want 10 - 10u sections in a micro-centrifuge tube. ?The only way to get the sections in the tube is for the sections to roll. ?How do you get sections to roll when you want them to roll? ?I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. ?When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Thu Jun 12 10:08:22 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Jun 12 10:08:27 2014 Subject: [Histonet] RE: rolling sections In-Reply-To: <321366197.150096.1402585177602.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <321366197.150096.1402585177602.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00278ED10667@CVM76.vetmed.wsu.edu> Hi Roberta, Check with those requesting to see if you can cut the equilivant but with thicker sections that will roll- like 5 20's or 2 50's. The rolls are lots easier to get into the tubes. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Thursday, June 12, 2014 8:00 AM To: Helen Fedor Cc: Histonet (histonet@lists.utsouthwestern.edu); Roberta Horner Subject: Re: [Histonet] RE: rolling sections That is how we do it also.? Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll.? If they are doing DNA or RNAase ou may need to wear glove to prevent any contamination of the blocks.? Pam Marcum UAMS ----- Original Message ----- From: "Helen Fedor" To: "Roberta Horner" , "Histonet (histonet@lists.utsouthwestern.edu)" Sent: Thursday, June 12, 2014 9:54:45 AM Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. ?They want 10 - 10u sections in a micro-centrifuge tube. ?The only way to get the sections in the tube is for the sections to roll. ?How do you get sections to roll when you want them to roll? ?I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. ?When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Thu Jun 12 11:08:10 2014 From: azdudley <@t> hotmail.com (anita) Date: Thu Jun 12 11:08:13 2014 Subject: [Histonet] Processors again Message-ID: Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all. Thanks a bunch!!! Have a great day!!! Anita Dudley Providence Hospital Mobile, Al. From dphillips <@t> vetmed.lsu.edu Thu Jun 12 11:08:07 2014 From: dphillips <@t> vetmed.lsu.edu (Del Phillips) Date: Thu Jun 12 11:08:37 2014 Subject: [Histonet] Please remove me Message-ID: <003801cf8658$7f91a850$7eb4f8f0$@vetmed.lsu.edu> Please remove me From rmire <@t> cvpath.org Thu Jun 12 11:13:20 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Thu Jun 12 11:13:26 2014 Subject: [Histonet] Processors again In-Reply-To: References: Message-ID: <8E38A568-1242-4696-8658-FEED69688AD5@cvpath.org> I do not like the Excelsior, not very user friendly. The VIP is good but my favorite is the Leica Peloris On Jun 12, 2014, at 12:08 PM, anita wrote: > Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all. > > > > Thanks a bunch!!! > > > > Have a great day!!! > > > > Anita Dudley > > Providence Hospital > > Mobile, Al. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Thu Jun 12 11:20:06 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Jun 12 11:20:20 2014 Subject: [Histonet] Processors again In-Reply-To: <8E38A568-1242-4696-8658-FEED69688AD5@cvpath.org> References: <8E38A568-1242-4696-8658-FEED69688AD5@cvpath.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012403B041@Mail2Node2.ad.uams.edu> We have 4 Excelsiors and love them. We also have one Leica ASP300 and it is a great workhorse for us. I think it just depends on your lab and what you like or what serves you best. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire Sent: Thursday, June 12, 2014 11:13 AM To: anita Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Processors again I do not like the Excelsior, not very user friendly. The VIP is good but my favorite is the Leica Peloris On Jun 12, 2014, at 12:08 PM, anita wrote: > Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all. > > > > Thanks a bunch!!! > > > > Have a great day!!! > > > > Anita Dudley > > Providence Hospital > > Mobile, Al. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Joyce.Weems <@t> emoryhealthcare.org Thu Jun 12 11:20:32 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jun 12 11:20:43 2014 Subject: [Histonet] Please remove me In-Reply-To: <003801cf8658$7f91a850$7eb4f8f0$@vetmed.lsu.edu> References: <003801cf8658$7f91a850$7eb4f8f0$@vetmed.lsu.edu> Message-ID: Hi Don, You must do that yourself at http://lists.utsouthwestern.edu/mailman/listinfo/histonet Thanks and best wishes! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Del Phillips Sent: Thursday, June 12, 2014 12:08 PM To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu; J Hawke Subject: [Histonet] Please remove me Please remove me _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From lpwenk <@t> sbcglobal.net Thu Jun 12 11:21:42 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jun 12 11:21:48 2014 Subject: [Histonet] rolling sections In-Reply-To: References: Message-ID: Try changing the angle of the knife blade, so that the clearance angle (angle between the knife blade and the block face) is larger. In other words, tip the top of the blade towards the block more. If there are numbers on the side of the knife holder, you want to move it to a larger number (like from 5 to 10). Before sectioning, remember to move the block holder towards you, since the blade will now be closer to the block, and you don't want to ker-chunk the block. And remember, when done, to return the clearance angle back to it's usual location, so you aren't curling all your ribbons. So look at the number it is usually set at, or, if there is no number, make some marks on the side of the knife holder, that you can line up again. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Thu Jun 12 11:45:11 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Jun 12 11:45:16 2014 Subject: [Histonet] Vantange/ block printer In-Reply-To: <817715931.1523521.1398806900934.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> References: <761E2B5697F795489C8710BCC72141FF3677D39A@ex07.net.ucsf.edu> <817715931.1523521.1398806900934.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: <5A33C952BB67F4468AF1F36D739212BC01124EC5DE@JERRY.Gia.com> For those of you who have Vantage, which block printer do you use? I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions? Thanks! From mucram11 <@t> comcast.net Thu Jun 12 11:48:44 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Jun 12 11:48:51 2014 Subject: [Histonet] Vantange/ block printer In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC01124EC5DE@JERRY.Gia.com> Message-ID: <2090207285.151194.1402591724456.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Thermo also has a cassette printer that works well.? We have t he cassette printer (6 hoppers per unit) and slide writers that work for us.? Pam Marcum ----- Original Message ----- From: "Amber McKenzie" Cc: "Histonet" Sent: Thursday, June 12, 2014 11:45:11 AM Subject: [Histonet] Vantange/ block printer For those of you who have Vantage, which block printer do you use? ?I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. ?I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Jun 12 11:59:21 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Jun 12 11:59:36 2014 Subject: [Histonet] RE: Vantange/ block printer In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC01124EC5DE@JERRY.Gia.com> References: <761E2B5697F795489C8710BCC72141FF3677D39A@ex07.net.ucsf.edu> <817715931.1523521.1398806900934.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> <5A33C952BB67F4468AF1F36D739212BC01124EC5DE@JERRY.Gia.com> Message-ID: <761E2B5697F795489C8710BCC72141FF36787853@ex07.net.ucsf.edu> Amber we got the Leica inkjet printer (6 actually). The 2D code reads very well. It prints at 5-7 seconds per cassette (depends on how much text you cram on there!). It is about the fastest printer out there. Data General is about the same speed, if you print with a reverse image ( black background- it uses the resin-coated cassette and laser ablation to form the image), slower with positive image (10-12 seconds - has to remove more of the black resin) . The TBS is slower because it uses a stylus to etch the plastic. Thermo printmate is also much slower (20 seconds per cassette in our tests). A surprise to me was that the cured ink from the Leica is also the most durable of all we tested. Very difficult to scratch off. The only drawback is that it is HUGE. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, June 12, 2014 9:45 AM Cc: Histonet Subject: [Histonet] Vantange/ block printer For those of you who have Vantage, which block printer do you use? I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions? Thanks! From suetp918 <@t> comcast.net Thu Jun 12 12:31:22 2014 From: suetp918 <@t> comcast.net (Sue) Date: Thu Jun 12 12:31:39 2014 Subject: [Histonet] Processors again In-Reply-To: Message-ID: <1658131920.284077.1402594282934.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> I used the Logs quick processor and it was really nice for small biopsies, no experience with their routine processor.? Have 6 Excelsior's and love them. STP TJUH From Cwagner <@t> samaritanhospital.org Thu Jun 12 12:50:28 2014 From: Cwagner <@t> samaritanhospital.org (Candace J. Wagner) Date: Thu Jun 12 12:50:37 2014 Subject: [Histonet] Formalin in operating (surgery) rooms Message-ID: <1005CFAD0A252C4894B839AECE87A3F279757279@SAM340.samaritanhospital.org> Hello all out in Histoland, I had a surgery tech ask me if there was a specific amount of formalin allowed in the surgery rooms. I could not find anywhere any documentation on a specific amount. We supply our surgery dept. with the formalin they need, usually about 2 gallons in each room now, but just wondering if anyone has any idea if there is such a "specific" amount?? Thanks -CJ- ---------------------------------------------------------------------------------------------------------------- E-MAIL CONFIDENTIALITY NOTICE: The contents of this e-mail message and any attachments are intended solely for the addressee(s) and may contain confidential and/or legally privileged information. If you are not the intended recipient of this message or if this message has been addressed to you in error, please immediately alert the sender by reply e-mail and then delete this message and any attachments. If you are not the intended recipient, you are notified that any use, dissemination, distribution, copying, or storage of this message or any attachment is strictly prohibited. From awill.imdpathlab <@t> yahoo.com Thu Jun 12 12:54:58 2014 From: awill.imdpathlab <@t> yahoo.com (awill.imdpathlab@yahoo.com) Date: Thu Jun 12 12:57:59 2014 Subject: [Histonet] (no subject) Message-ID: <1402595698.40198.YahooMailAndroidMobile@web126104.mail.ne1.yahoo.com> Please remove me From tpodawiltz <@t> lrgh.org Thu Jun 12 13:00:58 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Jun 12 13:01:03 2014 Subject: [Histonet] RE: Formalin in operating (surgery) rooms In-Reply-To: <1005CFAD0A252C4894B839AECE87A3F279757279@SAM340.samaritanhospital.org> References: <1005CFAD0A252C4894B839AECE87A3F279757279@SAM340.samaritanhospital.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D9863863261531750A@LRGHEXVS1.practice.lrgh.org> We do not allow any formalin in the surgery rooms. They come to the Histology department to pre-fill their containers since they do not a fume hood in the OR. Those containers go into a utility room and a scrub tech will leave the surgical suite to place the collected specimen in the appropriate sized container. They had to start doing this several years ago after a spill in one the surgical suites. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Candace J. Wagner Sent: Thursday, June 12, 2014 1:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Formalin in operating (surgery) rooms Hello all out in Histoland, I had a surgery tech ask me if there was a specific amount of formalin allowed in the surgery rooms. I could not find anywhere any documentation on a specific amount. We supply our surgery dept. with the formalin they need, usually about 2 gallons in each room now, but just wondering if anyone has any idea if there is such a "specific" amount?? Thanks -CJ- ---------------------------------------------------------------------------------------------------------------- E-MAIL CONFIDENTIALITY NOTICE: The contents of this e-mail message and any attachments are intended solely for the addressee(s) and may contain confidential and/or legally privileged information. If you are not the intended recipient of this message or if this message has been addressed to you in error, please immediately alert the sender by reply e-mail and then delete this message and any attachments. If you are not the intended recipient, you are notified that any use, dissemination, distribution, copying, or storage of this message or any attachment is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From suetp918 <@t> comcast.net Thu Jun 12 13:26:43 2014 From: suetp918 <@t> comcast.net (Sue) Date: Thu Jun 12 13:27:00 2014 Subject: [Histonet] RE: Formalin in operating (surgery) rooms In-Reply-To: <38667E7FB77ECD4E91BFAEB8D9863863261531750A@LRGHEXVS1.practice.lrgh.org> Message-ID: <1686298406.285086.1402597603563.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> We do not allow formalin in the actualy OR, yet it is in the Ultrasound Suites.? There is a holding area outside the OR with the formalin, S Paturzo TJUH From Joyce.Weems <@t> emoryhealthcare.org Thu Jun 12 13:46:56 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jun 12 13:47:09 2014 Subject: [Histonet] (no subject) In-Reply-To: <1402595698.40198.YahooMailAndroidMobile@web126104.mail.ne1.yahoo.com> References: <1402595698.40198.YahooMailAndroidMobile@web126104.mail.ne1.yahoo.com> Message-ID: Hello, You will need to do that yourself at http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best wishes! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of awill.imdpathlab@yahoo.com Sent: Thursday, June 12, 2014 1:55 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Please remove me _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From vperez <@t> pathreflab.com Thu Jun 12 15:04:19 2014 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Thu Jun 12 15:04:31 2014 Subject: [Histonet] RE: Vantange/ block printer In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC01124EC5DE@JERRY.Gia.com> References: <761E2B5697F795489C8710BCC72141FF3677D39A@ex07.net.ucsf.edu> <817715931.1523521.1398806900934.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> <5A33C952BB67F4468AF1F36D739212BC01124EC5DE@JERRY.Gia.com> Message-ID: We use one from general data. Seems to work well with us, the only thing is you have to change the hopper when you want to change the cassette color. We have learned to just batch all the specimens together that have same color cassette. I believe you can get another model that has the rotating one that will change the hopper with the color you want (not sure though) Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, June 12, 2014 11:45 AM Cc: Histonet Subject: [Histonet] Vantange/ block printer For those of you who have Vantage, which block printer do you use? I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions? Thanks! From hlukey <@t> msn.com Thu Jun 12 17:02:42 2014 From: hlukey <@t> msn.com (Hugh Luk) Date: Thu Jun 12 17:02:46 2014 Subject: [Histonet] RE: Histonet Digest, Vol 127, Issue 9 In-Reply-To: References: Message-ID: Hi Ronda Mire, Your best bet is to find someone who discarded an old VIP and has kept their baskets when they purchased new machines, to sell you theirs. Sorry I don't fit this criteria anymore. We have thought about replacement parts too, but have not found baskets that work as well as the original manufacturer's items that take advantage of the limited retort space. You can buy metal autoclave baskets (ie. https://us.vwr.com/store/catalog/product.jsp?catalog_number=89259-814&_requestid=343525) (not affiliated with VWR) or even items meant for stationary for $20-$40 (not affiliated with being stationary either). However, caution with coatings and metal issues are warranted. Good luck from Hawaii, Hugh ---------------------------------------------------------------------- > Date: Fri, 6 Jun 2014 12:22:00 -0400 > From: Ronda Mire > Subject: [Histonet] cassette tissue baskets for Sakura VIP6 > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > > Hello Histonetters, > > Does anyone in Histoland have an off market source for the metal tissue processing baskets used in the Sakura VIP processor? I need to purchase several of the 150 cassette capacity and several of the 75 cassette capacity. They are super expensive directly from Sakura. > > Ronda Mire HT (ASCP) > Laboratory Manager > CVPath Institute > Gaithersburg, MD ------------------------------ From Pat.Patterson <@t> propath.com Thu Jun 12 20:11:17 2014 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Thu Jun 12 20:11:21 2014 Subject: [Histonet] IHC Supervisor - Dallas Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D1690F89C@Mail.propathlab.com> We have a current opening for an experienced IHC Supervisor to oversee the overnight operations of our high volume, high quality immunohistochemistry laboratory. ProPath is a progressive, CAP accredited, high-volume pathology practice located in Dallas, Texas. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, In Situ Hybridization procedures, antibody titer preparation, workflow management, equipment maintenance, QC/QA recording, employee assignments. Our desired candidate should have strong management abilities; organizational and training skills; a minimum of 5 years of experience in histology, 3 years of IHC experience and with at least one year of supervisory experience; as well as excellent written and oral communication skills. In addition, experience with Microsoft Office and database management is required. We also require (ASCP) HT or HTL certification, QIHC certification is encouraged. Minimum of Associates degree required. ProPath utilizes leading technology and is a quality oriented pathology practice. Competitive salary with a sign-on bonus and relocation assistance offered. Our benefits include vacation, group medical/dental, and disability insurance, as well as a matched 401(k) plan and more. To apply, please visit www.propath.com or contact me with any questions! Pat Patterson, HTL(ASCP) Manager, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From jmoreira <@t> sidra.org Fri Jun 13 00:57:55 2014 From: jmoreira <@t> sidra.org (Joana Moreira) Date: Fri Jun 13 00:58:17 2014 Subject: [Histonet] RE: rolling sections Message-ID: I agree - the sections don't need to roll. You can scrunch them and put them in the tube. I used to cut paraffin rolls / sections and manually extract DNA from them. You'll find that some roll very well some others not at all. My advice is to when you scrunch them, leave them more towards the bottom of the tube - often times when there's smaller pieces of wax in top part (and due to statics) upon opening the microtubes to start dewaxing there might be some pieces of wax coming out; same applies to smaller rolls. Also agree with the comment about increasing the thickness; however this might mean an extra step of xylene during the dewaxing process. And yes, do use and change gloves, blade and disinfect microtome sectioning area if the rolls are being used for nucleic acid extraction. Joana Joana Moreira Supervisor ? Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org ------------------------------ Message: 7 Date: Thu, 12 Jun 2014 14:54:45 +0000 From: Helen Fedor Subject: [Histonet] RE: rolling sections To: 'Roberta Horner' , "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 12 Jun 2014 14:58:03 +0000 From: Martha Ward-Pathology Subject: [Histonet] RE: rolling sections To: Helen Fedor , 'Roberta Horner' , "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We do the same thing on our lab. It isn't necessary for them to roll....we just catch them and fold them up and put them in the tube. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, June 12, 2014 10:55 AM To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 12 Jun 2014 14:58:46 +0000 From: Bernice Frederick Subject: [Histonet] RE: rolling sections To: Martha Ward-Pathology , Helen Fedor , "'Roberta Horner'" , "Histonet (histonet@lists.utsouthwestern.edu)" Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0D02D6@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="iso-8859-1" True. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, June 12, 2014 9:58 AM To: Helen Fedor; 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections We do the same thing on our lab. It isn't necessary for them to roll....we just catch them and fold them up and put them in the tube. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Thursday, June 12, 2014 10:55 AM To: 'Roberta Horner'; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 12 Jun 2014 14:59:37 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] RE: rolling sections To: Helen Fedor Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" , Roberta Horner Message-ID: <321366197.150096.1402585177602.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 That is how we do it also.?? Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll.?? If they are doing DNA or RNAase ou may need to wear glove to prevent any contamination of the blocks.?? Pam Marcum UAMS ----- Original Message ----- From: "Helen Fedor" To: "Roberta Horner" , "Histonet (histonet@lists.utsouthwestern.edu)" Sent: Thursday, June 12, 2014 9:54:45 AM Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. ??They want 10 - 10u sections in a micro-centrifuge tube. ??The only way to get the sections in the tube is for the sections to roll. ??How do you get sections to roll when you want them to roll? ??I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. ??When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 12 Jun 2014 15:08:22 +0000 From: "Truscott, Tom" Subject: RE: [Histonet] RE: rolling sections To: Pam Marcum , Helen Fedor Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" , Roberta Horner Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00278ED10667@CVM76.vetmed.wsu.edu> Content-Type: text/plain; charset="utf-8" Hi Roberta, Check with those requesting to see if you can cut the equilivant but with thicker sections that will roll- like 5 20's or 2 50's. The rolls are lots easier to get into the tubes. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Thursday, June 12, 2014 8:00 AM To: Helen Fedor Cc: Histonet (histonet@lists.utsouthwestern.edu); Roberta Horner Subject: Re: [Histonet] RE: rolling sections That is how we do it also.?? Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll.?? If they are doing DNA or RNAase ou may need to wear glove to prevent any contamination of the blocks.?? Pam Marcum UAMS ----- Original Message ----- From: "Helen Fedor" To: "Roberta Horner" , "Histonet (histonet@lists.utsouthwestern.edu)" Sent: Thursday, June 12, 2014 9:54:45 AM Subject: [Histonet] RE: rolling sections Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. Helen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. ??They want 10 - 10u sections in a micro-centrifuge tube. ??The only way to get the sections in the tube is for the sections to roll. ??How do you get sections to roll when you want them to roll? ??I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. ??When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 12 Jun 2014 11:08:10 -0500 From: anita Subject: [Histonet] Processors again To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all. Thanks a bunch!!! Have a great day!!! Anita Dudley Providence Hospital Mobile, Al. ------------------------------ Message: 13 Date: Thu, 12 Jun 2014 11:08:07 -0500 From: "Del Phillips" Subject: [Histonet] Please remove me To: , , "J Hawke" Message-ID: <003801cf8658$7f91a850$7eb4f8f0$@vetmed.lsu.edu> Content-Type: text/plain; charset="us-ascii" Please remove me ------------------------------ Message: 14 Date: Thu, 12 Jun 2014 12:13:20 -0400 From: Ronda Mire Subject: Re: [Histonet] Processors again To: anita Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: <8E38A568-1242-4696-8658-FEED69688AD5@cvpath.org> Content-Type: text/plain; charset=iso-8859-1 I do not like the Excelsior, not very user friendly. The VIP is good but my favorite is the Leica Peloris On Jun 12, 2014, at 12:08 PM, anita wrote: > Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all. > > > > Thanks a bunch!!! > > > > Have a great day!!! > > > > Anita Dudley > > Providence Hospital > > Mobile, Al. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 12 Jun 2014 16:20:06 +0000 From: "Marcum, Pamela A" Subject: RE: [Histonet] Processors again To: Ronda Mire , anita Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012403B041@Mail2Node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We have 4 Excelsiors and love them. We also have one Leica ASP300 and it is a great workhorse for us. I think it just depends on your lab and what you like or what serves you best. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire Sent: Thursday, June 12, 2014 11:13 AM To: anita Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Processors again I do not like the Excelsior, not very user friendly. The VIP is good but my favorite is the Leica Peloris On Jun 12, 2014, at 12:08 PM, anita wrote: > Sorry to bring this up again but wondering if anyone has used the Milestone Logos One? I have info on the Excelsior, and we curantly have a VIP. Just wanting to get ingo and thoughts on them all. > > > > Thanks a bunch!!! > > > > Have a great day!!! > > > > Anita Dudley > > Providence Hospital > > Mobile, Al. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 16 Date: Thu, 12 Jun 2014 16:20:32 +0000 From: "Weems, Joyce K." Subject: RE: [Histonet] Please remove me To: "'Del Phillips'" , "histonet@lists.utsouthwestern.edu" , "histonet-request@lists.utsouthwestern.edu" , J Hawke Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Don, You must do that yourself at http://lists.utsouthwestern.edu/mailman/listinfo/histonet Thanks and best wishes! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Del Phillips Sent: Thursday, June 12, 2014 12:08 PM To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu; J Hawke Subject: [Histonet] Please remove me Please remove me _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 17 Date: Thu, 12 Jun 2014 12:21:42 -0400 From: "Lee & Peggy Wenk" Subject: Re: [Histonet] rolling sections To: "Roberta Horner" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Try changing the angle of the knife blade, so that the clearance angle (angle between the knife blade and the block face) is larger. In other words, tip the top of the blade towards the block more. If there are numbers on the side of the knife holder, you want to move it to a larger number (like from 5 to 10). Before sectioning, remember to move the block holder towards you, since the blade will now be closer to the block, and you don't want to ker-chunk the block. And remember, when done, to return the clearance angle back to it's usual location, so you aren't curling all your ribbons. So look at the number it is usually set at, or, if there is no number, make some marks on the side of the knife holder, that you can line up again. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Roberta Horner Sent: Thursday, June 12, 2014 10:42 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] rolling sections I have some researchers that want to do PCR. They want 10 - 10u sections in a micro-centrifuge tube. The only way to get the sections in the tube is for the sections to roll. How do you get sections to roll when you want them to roll? I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons. When I want a thick ribbon it will roll, darn that Murphy and his laws. Roberta Horner Animal Diagnostic Lab Penn State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 12 Jun 2014 16:45:11 +0000 From: Amber McKenzie Subject: [Histonet] Vantange/ block printer Cc: Histonet Message-ID: <5A33C952BB67F4468AF1F36D739212BC01124EC5DE@JERRY.Gia.com> Content-Type: text/plain; charset="utf-8" For those of you who have Vantage, which block printer do you use? I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions? Thanks! ------------------------------ Message: 19 Date: Thu, 12 Jun 2014 16:48:44 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] Vantange/ block printer To: Amber McKenzie Cc: Histonet Message-ID: <2090207285.151194.1402591724456.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Content-Type: text/plain; charset=utf-8 Thermo also has a cassette printer that works well.?? We have t he cassette printer (6 hoppers per unit) and slide writers that work for us.?? Pam Marcum ----- Original Message ----- From: "Amber McKenzie" Cc: "Histonet" Sent: Thursday, June 12, 2014 11:45:11 AM Subject: [Histonet] Vantange/ block printer For those of you who have Vantage, which block printer do you use? ??I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. ??I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 12 Jun 2014 16:59:21 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: Vantange/ block printer To: "'Amber McKenzie'" Cc: Histonet Message-ID: <761E2B5697F795489C8710BCC72141FF36787853@ex07.net.ucsf.edu> Content-Type: text/plain; charset=utf-8 Amber we got the Leica inkjet printer (6 actually). The 2D code reads very well. It prints at 5-7 seconds per cassette (depends on how much text you cram on there!). It is about the fastest printer out there. Data General is about the same speed, if you print with a reverse image ( black background- it uses the resin-coated cassette and laser ablation to form the image), slower with positive image (10-12 seconds - has to remove more of the black resin) . The TBS is slower because it uses a stylus to etch the plastic. Thermo printmate is also much slower (20 seconds per cassette in our tests). A surprise to me was that the cured ink from the Leica is also the most durable of all we tested. Very difficult to scratch off. The only drawback is that it is HUGE. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, June 12, 2014 9:45 AM Cc: Histonet Subject: [Histonet] Vantange/ block printer For those of you who have Vantage, which block printer do you use? I've heard Leica has one with 3-6 hoppers that can print the 2D barcode on it pretty fast. I currently have the TBS block printer, but I'm told it can print the bar code, but very slow...Any suggestions? Thanks! ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 17 ***************************************** Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From Michael.LaFriniere <@t> ccplab.com Fri Jun 13 04:43:30 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Fri Jun 13 04:43:46 2014 Subject: [Histonet] RE: GMS better than PAS for Fungi In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157E00EC233@xmdb04.nch.kids> References: <6D6BD1DE8A5571489398B392A38A7157E00EB10B@xmdb04.nch.kids> <4A2A16B9707CE04E9CB6C82DC18C1D29673F4E@AHCMSASEXCH02.my.ahc.local> <6D6BD1DE8A5571489398B392A38A7157E00EC233@xmdb04.nch.kids> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29674819@AHCMSASEXCH02.my.ahc.local> Tony, Thank you for your explanation, however, I think the original question was from a histonetter seeking to eliminate Chromic Acid from their standard GMS stain due to it's abusive reaction problems noted in the microwave, and was inquiring ideas if anybody has experience with another solution less abusive and works well. A few of us responded that the periodic acid used as the "mordant" in place of the chromic acid has worked fine in micro wave staining and does not react in such a way chromic acid does. I think our goal is to help with demonstrating fungus that the pathologist may feel is demonstrated on the HE with the most efficient, and reliable way. Yes one may feel the PAS will do such however dependant on the type of fungus I agree the GMS would be a better choice, the comment that a few of us made was that periodic acid has demonstrated a replacement that works fine and doesn't seem to minimize fungal demonstration on the slide for the GMS stain and elimination of the Chromic acid, and yes this would most like change the abbreviation of the stain... Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Tuesday, June 10, 2014 8:50 PM To: Michael LaFriniere; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: GMS better than PAS for Fungi Hi Michael, The PASM uses periodic acid, followed by the methenamine silver reaction. This is usually for basement membranes, though some methods, including mine, uses a thiosemicarbazide step before the silver. GMS uses Chromic acid followed by the methenamine silver reaction. The idea of a GMS is not to demonstrate pseudo-fungi. These are usually PAS positive. We would rather not have patients treated with anti-fungals if they do not actually have a fungus infection. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Michael LaFriniere [mailto:Michael.LaFriniere@ccplab.com] Sent: Wednesday, 11 June 2014 2:41 AM To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu Subject: RE: GMS better than PAS for Fungi I must disagree Tony, with my experience,using Periodic acid instead of chromic acid in the GMS stain I have no problem demonstrating Pseudo fungi nor strong results demonstrating Pneumocystis, think you may be confusing stains, this is not a (PSAM)that I am aware of. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Wednesday, June 04, 2014 7:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS better than PAS for Fungi Thought that the subject title should be changed. (References available on request) No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that they are GMS positive whereas other claim they are GMS negative. My own experience is that they are GMS (using chromic acid) negative. It is possible that our pathologists aren't aware that the "GMS" that their lab does might be using Periodic acid instead of Chromic acid. Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of chromic acid. The literature often does not report the exact GMS used which makes clear understanding of the histochemical results difficult. Pneumocystis will not be easy to see unless chromic acid is used (the mucin stains strongly PAS (and hence PASM) positive obscuring the small microorganisms). Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas they stain quite well with GMS. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, 5 June 2014 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acid Clean Glassware Chromic acid does a nifty job of removing metal deposits on glassware, but so do many commercial lab detergents. Chromic acid is the oxidizer for the fungus in the GMS stain. Go one better and get rid of Chromic Acid out of your lab. It is probably one of the more toxic / nasty chemicals in your department. Instead, try Churukian's Ammoniacal Silver for Fungus in the microwave. It is a much simpler, faster, prettier stain. It uses Periodic Acid as the oxidizer and does not stain the elastic fibers like a regular GMS. Both you and your pathologists will love it, I promise. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. Acid Cleaned glassware (Abbott, Tanya) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Jun 13 06:44:53 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Jun 13 06:44:58 2014 Subject: [Histonet] Formalin in operating (surgery) rooms In-Reply-To: <1005CFAD0A252C4894B839AECE87A3F279757279@SAM340.samaritanhospital.org> References: <1005CFAD0A252C4894B839AECE87A3F279757279@SAM340.samaritanhospital.org> Message-ID: <34E02CE4482E41058EABB0C4B9656605@HP2010> I think this is mostly a safety issue, and suggest NOT allowing any amount of formalin in OR/surgery rooms. 1. Training: Doesn't matter how much or how little formalin is in the room. If it is being used in a room, then everyone using it MUST receive yearly training on formaldehyde and on spill kits, according to OSHA. So anyone who picks up the tissue and puts it in a container with formalin must be trained yearly - every tech, every nurse, etc. That can be a LOT of people. Who is going to do the training and the documentation? 2. Spill Kits: If there is formalin in the OR rooms, there must be formaldehyde spill kits in the room, or very, very close by the room. And everyone one working in the OR must know where the kits are and how to use them (training). This not practical inside each OR room (no space, sterilization, etc.), so there are usually kits very near by each OR. That would usually mean having one kit for every X number of OR's, with wall signs marking their locations. Are there enough "nursing stations", cleaning rooms, spaces in hall, etc. to position spill kits, to have enough kits available close by all the rooms? 3. Spill: If there is a formalin spill in the OR - I don't even want to think about evacuating everyone from the OR, including the patient who is opened up on the table. The better idea is to have one or a couple of locations (separate rooms) where the formalin is stored, and then bring the tissue to those locations, and place the tissue in the formalin at those locations. Then you have to train just those people pouring the formalin on the tissues in those locations, and it would be easier to store spill kits and contain the spills. Some hospitals don't allow formalin on the OR floor. They have refrigerators in rooms near the OR, where the tissue is stored fresh after removed from surgery. Then every hour or two, all the tissue is taken to the lab (either the OR has runners, or the lab has runners). There is a documentation issue - have to write down what tissue is dropped off in the refrigerators and when, by whom, and then what tissue was picked up, when and by whom. Tissue can easily be overlooked, and left in the refrigerator for a long period of time. Peggy A. Wenk, HTL(ASCP) -----Original Message----- From: Candace J. Wagner Sent: Thursday, June 12, 2014 1:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Formalin in operating (surgery) rooms Hello all out in Histoland, I had a surgery tech ask me if there was a specific amount of formalin allowed in the surgery rooms. I could not find anywhere any documentation on a specific amount. We supply our surgery dept. with the formalin they need, usually about 2 gallons in each room now, but just wondering if anyone has any idea if there is such a "specific" amount?? Thanks -CJ- ---------------------------------------------------------------------------------------------------------------- E-MAIL CONFIDENTIALITY NOTICE: The contents of this e-mail message and any attachments are intended solely for the addressee(s) and may contain confidential and/or legally privileged information. If you are not the intended recipient of this message or if this message has been addressed to you in error, please immediately alert the sender by reply e-mail and then delete this message and any attachments. If you are not the intended recipient, you are notified that any use, dissemination, distribution, copying, or storage of this message or any attachment is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dphillips <@t> vetmed.lsu.edu Fri Jun 13 07:17:16 2014 From: dphillips <@t> vetmed.lsu.edu (Del Phillips) Date: Fri Jun 13 07:18:05 2014 Subject: [Histonet] remove name Message-ID: <008101cf8701$75450b90$5fcf22b0$@vetmed.lsu.edu> Please remove my name for the histonet list. Del Phillips dphillips@vetmed.lsu.edu From cheastys <@t> svm.vetmed.wisc.edu Fri Jun 13 07:25:32 2014 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Fri Jun 13 07:25:37 2014 Subject: [Histonet] Temp Histologist Needed! Madison Wisconsin Message-ID: Hello all, My lab needs a temp Histologist from approximately June 25 - August 15th. (One of my techs had a bicycle accident and broke his collarbone among other things, and there are several vacations coming up for other staff as well.) I would appreciate suggestion of reputable temp agencies; I haven't needed to use one since I worked in Anchorage, and that was last century! Thanks for your help! Sandy Sandra Cheasty Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From Joyce.Weems <@t> emoryhealthcare.org Fri Jun 13 07:37:04 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jun 13 07:37:14 2014 Subject: [Histonet] remove name In-Reply-To: <008101cf8701$75450b90$5fcf22b0$@vetmed.lsu.edu> References: <008101cf8701$75450b90$5fcf22b0$@vetmed.lsu.edu> Message-ID: Hi Del, You must do that yourself at http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best wishes! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Del Phillips Sent: Friday, June 13, 2014 8:17 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] remove name Please remove my name for the histonet list. Del Phillips dphillips@vetmed.lsu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rmire <@t> cvpath.org Fri Jun 13 08:13:45 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Fri Jun 13 08:13:54 2014 Subject: [Histonet] Re: Histonet Digest, Vol 127, Issue 9 In-Reply-To: References: Message-ID: <19CD34D1-EF73-4551-A150-848F0A4E8046@cvpath.org> Thanks Hugh. On Jun 12, 2014, at 6:02 PM, Hugh Luk wrote: > Hi Ronda Mire, > > Your best bet is to find someone who discarded an old VIP and has kept their baskets when they purchased new machines, to sell you theirs. Sorry I don't fit this criteria anymore. > > We have thought about replacement parts too, but have not found baskets that work as well as the original manufacturer's items that take advantage of the limited retort space. > > You can buy metal autoclave baskets (ie. https://us.vwr.com/store/catalog/product.jsp?catalog_number=89259-814&_requestid=343525) (not affiliated with VWR) or even items meant for stationary for $20-$40 (not affiliated with being stationary either). However, caution with coatings and metal issues are warranted. > > Good luck from Hawaii, > Hugh > > ---------------------------------------------------------------------- > > Date: Fri, 6 Jun 2014 12:22:00 -0400 > > From: Ronda Mire > > Subject: [Histonet] cassette tissue baskets for Sakura VIP6 > > To: histonet@lists.utsouthwestern.edu > > Message-ID: > > Content-Type: text/plain; charset=us-ascii > > > > > > Hello Histonetters, > > > > Does anyone in Histoland have an off market source for the metal tissue processing baskets used in the Sakura VIP processor? I need to purchase several of the 150 cassette capacity and several of the 75 cassette capacity. They are super expensive directly from Sakura. > > > > Ronda Mire HT (ASCP) > > Laboratory Manager > > CVPath Institute > > Gaithersburg, MD > ------------------------------ > From lpwenk <@t> sbcglobal.net Fri Jun 13 08:55:55 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Jun 13 08:56:06 2014 Subject: [Histonet] Formalin in operating (surgery) rooms In-Reply-To: <34E02CE4482E41058EABB0C4B9656605@HP2010> References: <1005CFAD0A252C4894B839AECE87A3F279757279@SAM340.samaritanhospital.org> <34E02CE4482E41058EABB0C4B9656605@HP2010> Message-ID: <91670CAE3D1144679F64C7C9240952D7@HP2010> Forgot to add: 4. Formaldehyde Monitoring: Each OR room would have to have formaldehyde monitoring of all positions involved in the handling of the formalin. This can be time consuming and expensive, to have to do this for each OR (who says the ventilation flow is the same in each OR?). It would be easier to monitor if the formalin was in one (or a few) room(s) outside of the OR, with only a few people involved in pouring the formalin into the containers with the tissue. Or, if only fresh tissue was stored in a refrigerator outside the OR, then no monitoring of formaldehyde would need to be done in the OR area. So in other words, there are not "rules" as to how much formalin can be in an OR, just like there are not "rules" as to how much formalin can be stored in a lab. There is the OSHA Formaldehyde Standard that must be followed (29CFR1910.1480). And there there are all kinds of complicated storage regs as to amount of formalin, size of room, ventilation rate through room, etc. It would just be so much easier if NO formalin was allowed in the OR rooms. Peggy A. Wenk, HTL(ASCP) -----Original Message----- From: Lee & Peggy Wenk Sent: Friday, June 13, 2014 7:44 AM To: Candace J. Wagner ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin in operating (surgery) rooms I think this is mostly a safety issue, and suggest NOT allowing any amount of formalin in OR/surgery rooms. 1. Training: Doesn't matter how much or how little formalin is in the room. If it is being used in a room, then everyone using it MUST receive yearly training on formaldehyde and on spill kits, according to OSHA. So anyone who picks up the tissue and puts it in a container with formalin must be trained yearly - every tech, every nurse, etc. That can be a LOT of people. Who is going to do the training and the documentation? 2. Spill Kits: If there is formalin in the OR rooms, there must be formaldehyde spill kits in the room, or very, very close by the room. And everyone one working in the OR must know where the kits are and how to use them (training). This not practical inside each OR room (no space, sterilization, etc.), so there are usually kits very near by each OR. That would usually mean having one kit for every X number of OR's, with wall signs marking their locations. Are there enough "nursing stations", cleaning rooms, spaces in hall, etc. to position spill kits, to have enough kits available close by all the rooms? 3. Spill: If there is a formalin spill in the OR - I don't even want to think about evacuating everyone from the OR, including the patient who is opened up on the table. The better idea is to have one or a couple of locations (separate rooms) where the formalin is stored, and then bring the tissue to those locations, and place the tissue in the formalin at those locations. Then you have to train just those people pouring the formalin on the tissues in those locations, and it would be easier to store spill kits and contain the spills. Some hospitals don't allow formalin on the OR floor. They have refrigerators in rooms near the OR, where the tissue is stored fresh after removed from surgery. Then every hour or two, all the tissue is taken to the lab (either the OR has runners, or the lab has runners). There is a documentation issue - have to write down what tissue is dropped off in the refrigerators and when, by whom, and then what tissue was picked up, when and by whom. Tissue can easily be overlooked, and left in the refrigerator for a long period of time. Peggy A. Wenk, HTL(ASCP) -----Original Message----- From: Candace J. Wagner Sent: Thursday, June 12, 2014 1:50 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Formalin in operating (surgery) rooms Hello all out in Histoland, I had a surgery tech ask me if there was a specific amount of formalin allowed in the surgery rooms. I could not find anywhere any documentation on a specific amount. We supply our surgery dept. with the formalin they need, usually about 2 gallons in each room now, but just wondering if anyone has any idea if there is such a "specific" amount?? Thanks -CJ- ---------------------------------------------------------------------------------------------------------------- E-MAIL CONFIDENTIALITY NOTICE: The contents of this e-mail message and any attachments are intended solely for the addressee(s) and may contain confidential and/or legally privileged information. If you are not the intended recipient of this message or if this message has been addressed to you in error, please immediately alert the sender by reply e-mail and then delete this message and any attachments. If you are not the intended recipient, you are notified that any use, dissemination, distribution, copying, or storage of this message or any attachment is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Fri Jun 13 12:52:43 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Jun 13 12:52:44 2014 Subject: [Histonet] RE: Formalin in the OR In-Reply-To: <20140613161434.5EAE21E806F@trendmess-svr.holyredeemer.local> References: <20140613161434.5EAE21E806F@trendmess-svr.holyredeemer.local> Message-ID: Wow, this is such a safety issue with an accident waiting to happen. I totally agree with Peggy that Formalin should not be allowed in an OR room. Even a gallon spill would be cause to evacuate and can you imagine the consequences of that? We have a small room off of the OR suites stocked with a 5 gallon carboy over a 5 gal spill container Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. Re: Formalin in operating (surgery) rooms (Lee & Peggy Wenk) -----Original Message----- From: Lee & Peggy Wenk Sent: Friday, June 13, 2014 7:44 AM To: Candace J. Wagner ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin in operating (surgery) rooms I think this is mostly a safety issue, and suggest NOT allowing any amount of formalin in OR/surgery rooms. --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Lesley.Bechtold <@t> jax.org Fri Jun 13 13:11:37 2014 From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold) Date: Fri Jun 13 13:11:42 2014 Subject: [Histonet] Slide printers and Cassette Printers Message-ID: <08997064E2075247A4DD5A035DB51CDF64F9DD9C@jaxbhexms02.jax.org> Good afternoon, We are in the market for new slide and cassette printers. I'd be interested in hearing about what people like or don't like about their printers. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From koellingr <@t> comcast.net Fri Jun 13 15:31:10 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Jun 13 15:31:31 2014 Subject: [Histonet] RE: Formalin in the OR In-Reply-To: References: <20140613161434.5EAE21E806F@trendmess-svr.holyredeemer.local> Message-ID: <1422913479.3008217.1402691470272.JavaMail.root@comcast.net> Heartbreakingly sad, ? I do not know where the current regulations are but safety, as Terri rightly pointed out, is an accident that did happen.? Not an anecdote, you can look up March 1985, Jackson Memorial Hospital in Miami (years after I left). Patient went to surgery, had some cerebrospinal fluid (CSF) removed during operation?but an UNMARKED?container of gluteraldehyde (aldehyde) fixative got marked as CSF with all the comings and goings over many hours. When the CSF was set to be reinjected as replacement, the fixative got reinjected as replacement instead of his CSF.? Patient obviously died.? Can't believe that is the only actual safety issue that has ever cropped up?with surgery and formalin. ? So maybe a warning for both; ?no unlabeled bottles and no fixative right in the actual surgery suite. ? Ray Seattle WA ----- Original Message ----- From: "Terri Braud" To: histonet@lists.utsouthwestern.edu Sent: Friday, June 13, 2014 10:52:43 AM Subject: [Histonet] RE: Formalin in the OR Wow, this is such a safety issue with an accident waiting to happen. ?I totally agree with Peggy that Formalin should not be allowed in an OR room. ?Even a gallon spill would be cause to evacuate and can you imagine the consequences of that? We have a small room off of the OR suites stocked with a 5 gallon carboy over a 5 gal spill container Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. Re: Formalin in operating (surgery) rooms (Lee & Peggy Wenk) -----Original Message----- From: Lee & Peggy Wenk Sent: Friday, June 13, 2014 7:44 AM To: Candace J. Wagner ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin in operating (surgery) rooms I think this is mostly a safety issue, and suggest NOT allowing any amount of formalin in OR/surgery rooms. --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Jun 14 09:03:22 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Jun 14 09:03:28 2014 Subject: [Histonet] RE: Formalin in the OR In-Reply-To: <1422913479.3008217.1402691470272.JavaMail.root@comcast.net> References: <20140613161434.5EAE21E806F@trendmess-svr.holyredeemer.local>, , <1422913479.3008217.1402691470272.JavaMail.root@comcast.net> Message-ID: Thank you for your story about this patient event with formalin in the OR. I am sometimes confronted with the response that I am overly detailed about things and particularly with regulations and safety. If you have never experienced something like this, it is easy to get lax and expect that it will never occur.This is a good reminder, that while mistakes like this one may be infrequent, when they do happen it can be terribly tragic. No one ever wants to be involved with anything remotely similar to the circumstance you describe. In my opinion, just best to do everything you can think of to not even invite the possibility. Keep the formalin where you can limit the handlers and potential mix ups as much as possible! Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 13 Jun 2014 20:31:10 +0000 > From: koellingr@comcast.net > To: tbraud@holyredeemer.com > Subject: Re: [Histonet] RE: Formalin in the OR > CC: histonet@lists.utsouthwestern.edu > > Heartbreakingly sad, > > I do not know where the current regulations are but safety, as Terri rightly pointed out, is an accident that did happen. Not an anecdote, you can look up March 1985, Jackson Memorial Hospital in Miami (years after I left). > Patient went to surgery, had some cerebrospinal fluid (CSF) removed during operation but an UNMARKED container of gluteraldehyde (aldehyde) fixative got marked as CSF with all the comings and goings over many hours. When the CSF was set to be reinjected as replacement, the fixative got reinjected as replacement instead of his CSF. Patient obviously died. Can't believe that is the only actual safety issue that has ever cropped up with surgery and formalin. > > So maybe a warning for both; no unlabeled bottles and no fixative right in the actual surgery suite. > > Ray > Seattle WA > > ----- Original Message ----- > > From: "Terri Braud" > To: histonet@lists.utsouthwestern.edu > Sent: Friday, June 13, 2014 10:52:43 AM > Subject: [Histonet] RE: Formalin in the OR > > Wow, this is such a safety issue with an accident waiting to happen. I > totally agree with Peggy that Formalin should not be allowed in an OR > room. Even a gallon spill would be cause to evacuate and can you > imagine the consequences of that? > We have a small room off of the OR suites stocked with a 5 gallon carboy > over a 5 gal spill container > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > 2. Re: Formalin in operating (surgery) rooms (Lee & Peggy Wenk) > > -----Original Message----- > From: Lee & Peggy Wenk > Sent: Friday, June 13, 2014 7:44 AM > To: Candace J. Wagner ; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Formalin in operating (surgery) rooms > > I think this is mostly a safety issue, and suggest NOT allowing any > amount of formalin in OR/surgery rooms. > > > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it was sent. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BJohnson <@t> pbmlabs.com Sat Jun 14 12:43:57 2014 From: BJohnson <@t> pbmlabs.com (Brian Johnson) Date: Sat Jun 14 12:47:08 2014 Subject: [Histonet] Re: Slide printers and Cassette Printers In-Reply-To: <20140614165921.B127D6803B@TXLDCLAPPP01.MEDFUSIONSVS.NET> References: <20140614165921.B127D6803B@TXLDCLAPPP01.MEDFUSIONSVS.NET> Message-ID: We use both the 12 hopper and single hopper General Data cassette engravers. They are fast, and have been rock-solid in terms of reliability. The cost of consumables is high (you must use their cassettes), but due to the technology used, the engraved blocks read 100% of the time for our specimen tracking and real time labeling applications. Brian Johnson Director of Business Operations and IT PBM Labs Bjohnson@pbmlabs.com > PBM Labs PRIVACY AND CONFIDENTIALITY NOTICE: The information transmitted in this message and any attachments is intended for the person or entity to which it is addressed and may contain proprietary, confidential, and/or privileged material, which may include individually identifiable health information that requires safeguarding in compliance with the Health Insurance Portability and Accountability Act of 1996 as well as other federal and state laws. Any review, duplication, retransmission, distribution, or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you have received this message in error, please notify the sender immediately by return e-mail and delete all of the information from all computers or systems.? From sbreckenridge <@t> caperegional.com Mon Jun 16 07:27:18 2014 From: sbreckenridge <@t> caperegional.com (Breckenridge, Sue) Date: Mon Jun 16 07:27:30 2014 Subject: [Histonet] Full Time Histologist (HT or HTL ASCP) Message-ID: Hi all, There is a full time histologist position available in the beautiful southern shore area of New Jersey! The hospital, Cape Regional Medical Center, is close to beaches and provides a great work environment. The listing is as follows: FT position available, Monday through Friday, flexible hours, HT or HTL to perform routine histology, frozen sectioning, immunohistochemistry procedures, special stains, cytology preparation, grossing and cutting. Candidate must possess proven experience in all areas of histopathology and full developed organizational skills and communication skills are a must. Position requires rotating call coverage. Requirements: Certified/Registered or registry eligible through ASCP, or eligible. Must have good communication skills and ability to work with a multidisciplinary team to include physicians, management and supervisors, etc. Ability to move heavy objects; stand and walk for most of the working day. May be exposed to dangerous toxins, waste material, odors or fumes. An on-line application process is available. If you have any further questions about particulars, please contact: Human Resources Cape Regional Medical Center Phone: 609-463-2170 Thanks, Sue Breckenridge, BS, HT/HTL (ASCP) Histology Supervisor Cape Regional Medical Center Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED.The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. From christina.wolfe <@t> bms.com Mon Jun 16 15:35:53 2014 From: christina.wolfe <@t> bms.com (Wolfe, Christina) Date: Mon Jun 16 15:35:59 2014 Subject: [Histonet] Lendrum's stain for Paneth Cells Message-ID: Hi, Does anyone have a PMID for Lendrum's original paper " LENDRUM, A.C. 1947. The Phloxine-tartrazine method as a general histological stain and for the demonstration of inclusion bodies. Journal of Pathology and Bacteriology."? I have searched PubMed and I am not getting any results. We are having trouble getting this stain to work in our lab. The Paneth cells are not staining and there is no yellow stain at all with the tartrazine. We are trying to stain rat ileum. Anyone with experience using this stain - please help. :) Thanks! Kristie Christina Wolfe, BS, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From Betty.Gast <@t> hshs.org Mon Jun 16 16:34:10 2014 From: Betty.Gast <@t> hshs.org (Gast, Betty L) Date: Mon Jun 16 16:34:17 2014 Subject: [Histonet] HT/HTL job in Green Bay WI Message-ID: <468705ED810A34468A8650CFC201A800B45F08CC@EWJMB002.EW.HSHSAD.ORG> We have a full time job opening at St. Mary's Medical Center in Green Bay. Please check out at stmgb.org or hshs.org. Thanks! LEGAL DISCLAIMER: This message and all attachments may be confidential or protected by privilege. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the information contained in or attached to this message is strictly prohibited. Please notify the sender of the delivery error by replying to this message, and then delete it from your system. Thank you. From algranth <@t> email.arizona.edu Mon Jun 16 16:41:27 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Jun 16 16:41:31 2014 Subject: [Histonet] Lendrum's stain for Paneth Cells In-Reply-To: References: Message-ID: <62779D94-C3AF-46CB-8E65-BEA5A9E5726F@email.arizona.edu> I just did this stain again and it was beautiful. I used Lendrum's protocol from Stains File web site. http://stainsfile.info/StainsFile/stain/micro/phloxtartlend.htm When I got to step 5 I proceeded one slide at a time and checked under the microscope to get the differentiation to where I wanted it. The time varies for each slide. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From lpwenk <@t> sbcglobal.net Mon Jun 16 16:54:44 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jun 16 16:54:51 2014 Subject: [Histonet] Lendrum's stain for Paneth Cells In-Reply-To: References: Message-ID: <40150C36A98944248641576346719651@HP2010> This is from the "Theory and Practice of HIstotechnology", 2nd edition, 190. Dezna C. Sheehan and Barbara B. Hrapchak. The reference for their procedure is the article you mentioned. FIXATIVE: Mercuric formalin preferred, but 10% NBF can be used SOLUTIONS: Mayer Hemaxylin Phloxine stain - 1 g Phloxine - 200 m of 70% Ethanol - 1 g Calcium chloride Tartrazine solution - 2.5 g Tartrazine - 100 mL cellosolve (ethylene glycol monoethyl ether) PROCEDURE: 1. Deparaffinize and hydrate slides to d. water 2. Stain for 5-10 minutes in Mayer hematoxylin 3. Blue sections in running tap water for 15 minutes 4. Stain with phloxine solution for 30 minutes 5. Rinse briefly in distilled water, and drain on filter paper 6. Differentiate with tartrazine solution until the inclusion bodies stand out bright red and the background is yellow. 7. Dehydrate; clear in xylene; coverslip using a synthetic mounting media RESULTS: Inclusion bodies = red Nuclei = blue Background - yellow NOTES FROM PEGGY: 1. Seems like you should be able to use Gill regressive or Harris regressive, too. At the time we did this stain 30 years ago, our lab was using Mayer hematoxylin. 2. It's very similar to doing a Brown and Hopps, or a Brown and Brenn. Not enough time in the tartrazine differentiator, and the background will be reddish, making is hard to find the inclusion bodies. Too much time in the tartrazine differentiator, and the background is yellow but so are the inclusion bodies. I remember doing this for something decades ago, and remember how easy it was to over and under differentiate. 3. I seem to remember reusing the staining solutions multiple times. 4. Yes, we used cellosolve to make the tartrazine. It wasn't the only staining solution that used it, so we had it around. I'm wondering if you could use something like acetone instead, but do a much faster differentiation. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Wolfe, Christina Sent: Monday, June 16, 2014 4:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lendrum's stain for Paneth Cells Hi, Does anyone have a PMID for Lendrum's original paper " LENDRUM, A.C. 1947. The Phloxine-tartrazine method as a general histological stain and for the demonstration of inclusion bodies. Journal of Pathology and Bacteriology."? I have searched PubMed and I am not getting any results. We are having trouble getting this stain to work in our lab. The Paneth cells are not staining and there is no yellow stain at all with the tartrazine. We are trying to stain rat ileum. Anyone with experience using this stain - please help. :) Thanks! Kristie Christina Wolfe, BS, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From loveyadams40 <@t> yahoo.com Tue Jun 17 07:00:47 2014 From: loveyadams40 <@t> yahoo.com (loveyadams40@yahoo.com) Date: Tue Jun 17 07:00:54 2014 Subject: [Histonet] Finding a good histology supplies company Message-ID: <1403006447.46005.YahooMailAndroidMobile@web142601.mail.bf1.yahoo.com> Good morning Histonetters, I need help finding a reasonable price cytology processor and reagents for the machine as well . Who's the best in our field? Thank you in advance Marie Adams Sent from Yahoo Mail on Android From wbenton <@t> cua.md Tue Jun 17 07:05:46 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Jun 17 07:10:32 2014 Subject: [Histonet] Finding a good histology supplies company In-Reply-To: <1403006447.46005.YahooMailAndroidMobile@web142601.mail.bf1.yahoo.com> References: <1403006447.46005.YahooMailAndroidMobile@web142601.mail.bf1.yahoo.com> Message-ID: <0B8979A204680A42B93A52B486088CD93938636859@CUAEXH1.GCU-MD.local> Cytospin via Fisher http://www.thermoscientific.com/content/tfs/en/product/cytospin-4-cytocentrifuge.html Thinprep by Hologic http://www.hologic.com/en/cytology/ Fisher has a similar instrument to the Thinprep (SureThin) that they are selling as well. http://www.fishersci.com/ecomm/servlet/fsproductdetail_10652_16861456__-1_0 See which one you may be interested in and then check with the vendors that sell refurb equipment. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of loveyadams40@yahoo.com [loveyadams40@yahoo.com] Sent: Tuesday, June 17, 2014 8:00 AM To: Histonet Subject: [Histonet] Finding a good histology supplies company Good morning Histonetters, I need help finding a reasonable price cytology processor and reagents for the machine as well . Who's the best in our field? Thank you in advance Marie Adams Sent from Yahoo Mail on Android _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Joyce.Weems <@t> emoryhealthcare.org Tue Jun 17 07:32:50 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jun 17 07:34:37 2014 Subject: [Histonet] Finding a good histology supplies company In-Reply-To: <1403006447.46005.YahooMailAndroidMobile@web142601.mail.bf1.yahoo.com> References: <1403006447.46005.YahooMailAndroidMobile@web142601.mail.bf1.yahoo.com> Message-ID: Hologic will work with you to provide good pricing if you are talking about ThinPrep methodology. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of loveyadams40@yahoo.com Sent: Tuesday, June 17, 2014 8:01 AM To: Histonet Subject: [Histonet] Finding a good histology supplies company Good morning Histonetters, I need help finding a reasonable price cytology processor and reagents for the machine as well . Who's the best in our field? Thank you in advance Marie Adams Sent from Yahoo Mail on Android _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From klaus.dern44 <@t> gmail.com Tue Jun 17 07:26:54 2014 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Tue Jun 17 07:34:44 2014 Subject: [Histonet] THICK AND THIN SECTIONS ? Message-ID: If you are using one of the following Microtomes and the advance mechanism is worn out ( too much play ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome, because these Models are no longer supported by the Manufacturer ( no parts availability ) Rather than replacing these excellent instruments, I have a PERMANENT solution to this problem. For Information contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com From srhthacker <@t> hotmail.com Tue Jun 17 07:44:07 2014 From: srhthacker <@t> hotmail.com (srhthacker@hotmail.com) Date: Tue Jun 17 07:44:17 2014 Subject: [Histonet] Peloris Message-ID: Is anyone using isopropanal on the Peloris? ? If so could you share your experience?? Thank you! Renee Sent on a Sprint Samsung Galaxy S? III From loveyadams40 <@t> yahoo.com Tue Jun 17 09:17:26 2014 From: loveyadams40 <@t> yahoo.com (loveyadams40@yahoo.com) Date: Tue Jun 17 09:17:31 2014 Subject: [Histonet] Cytology products Message-ID: <1403014646.15190.YahooMailAndroidMobile@web142605.mail.bf1.yahoo.com> Hi Histonetters, Has anyone used the non-gyn liquid - based cytology product QC science's NuMatrix thin-layer solution and their NuPrep enhancement solution. I need a procedure on using it and how to stain for PAP and AP. Is this a good product to use or is there something better out in our field. Thank you in advance Marie Adams Sent from Yahoo Mail on Android From loveyadams40 <@t> yahoo.com Tue Jun 17 09:18:42 2014 From: loveyadams40 <@t> yahoo.com (Lovetta Adams) Date: Tue Jun 17 09:18:51 2014 Subject: [Histonet] Finding a good histology supplies company In-Reply-To: Message-ID: <1403014722.96657.YahooMailAndroidMobile@web142604.mail.bf1.yahoo.com> Thank you for your input and help. Sincerely Lovetta Adams Sent from Yahoo Mail on Android From TanyaAbbott <@t> catholichealth.net Wed Jun 18 09:37:43 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Jun 18 09:37:49 2014 Subject: [Histonet] Back up Tissue processor Message-ID: <852F7D2C14FB464D80E182B15DB138AF30697EA3@CHIEX005.CHI.catholichealth.net> Does anyone keep a back up tissue processor in your lab? And if so, what sort of requirements are there for CAP for insuring it is working properly, etc? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From barbara.tibbs <@t> accuratediagnosticlabs.com Wed Jun 18 10:40:32 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Wed Jun 18 10:40:50 2014 Subject: [Histonet] RE: Back up Tissue processor In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF30697EA3@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF30697EA3@CHIEX005.CHI.catholichealth.net> Message-ID: <1403106004026.92938@accuratediagnosticlabs.com> You will need to validate whatever programs you have on the back-up processor. Run cassettes of tissue representative of what you usually process on a day to day basis. Embed, cut and stain with H&E and have your pathologist review the slides for satisfactory results. Document the programs, tissues and results. I create my own form for this. I would suggest if you're not going to use the processor on a daily basis that you run a "dummy" process (no cassettes) at least once a week to make sure the solutions are pumping into the retort. You don't want to be surprised with a broken back-up machine if your main processor is down! Hope this helps. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Abbott, Tanya Sent: Wednesday, June 18, 2014 1:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Back up Tissue processor Does anyone keep a back up tissue processor in your lab? And if so, what sort of requirements are there for CAP for insuring it is working properly, etc? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TanyaAbbott <@t> catholichealth.net Wed Jun 18 10:57:12 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Jun 18 10:57:18 2014 Subject: [Histonet] VIP 1000 Message-ID: <852F7D2C14FB464D80E182B15DB138AF306980C1@CHIEX005.CHI.catholichealth.net> To go along with my last question, does anyone know where I could get charcoal filters for the VIP 1000 tissue processor? I have a letter from 2002 from Sakura saying parts for this will probably only be available for 2 more years, so that's only 10 years ago,...........!! Assuming if I can't get filters, I can't run it anyway?! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From juan.l.bassett.ctr <@t> mail.mil Wed Jun 18 11:24:17 2014 From: juan.l.bassett.ctr <@t> mail.mil (Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US)) Date: Wed Jun 18 11:25:12 2014 Subject: [Histonet] control slides Message-ID: <5BEFD2C58946394F8E2A6EC3E1F86A9B15A31644@umechpanz.easf.csd.disa.mil> I am in need of control slides (already stained) of different type special stains for teaching purposes. H & E slides also needed. If you are in the Maryland area I may be able to pick up from your area. If out of state I'll pick up shipping . Juan l. Bassett HT Histotechnician Anatomical Div. National Museum of Health and Medicine 2500 Linden Lane Silver Spring, MD 20910 Office 301-319-3359 Lab 301-319-3328 Mobile 240-505-7247 Juan.l.bassett.ctr@mail.mil From jpiche <@t> wtbyhosp.org Wed Jun 18 11:36:33 2014 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Wed Jun 18 11:36:46 2014 Subject: [Histonet] Balance and Scale Calibration Message-ID: <631955447A364B45B9458D2905635110BADC7A63@WIN08-MBX-02.wtbyhosp.org> Hi Everyone, I was wondering what other anatomic pathology labs are doing to calibrate their scales and balances? I also would like to know what the basic tolerances for balances or scales is? I can only seem to find information in laboratories that deal with soil. I have a procedure and a calibration record sheet in place but I'm not sure what the range is for basic tolerances of the scale or balance so I'm not sure what to do if a scale or balance is not within the right limits. Thank you for any insight, procedures, information etc. Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From liz <@t> premierlab.com Wed Jun 18 12:02:52 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 18 12:02:59 2014 Subject: [Histonet] RE: Balance and Scale Calibration In-Reply-To: <631955447A364B45B9458D2905635110BADC7A63@WIN08-MBX-02.wtbyhosp.org> References: <631955447A364B45B9458D2905635110BADC7A63@WIN08-MBX-02.wtbyhosp.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD156@SBS2K8.premierlab.local> Jessica Your operating manual for your balance should list your level for tolerance and any good calibration company will also know what is acceptable, this tolerance level can also be governed by your SOP too. We have a Mettler Toledo Model AB204-S that measures out to 0.0000 the tolerance on our calibration report is +/- 0.0005. We are not a clinical lab but we have our balance calibrated once a year and we also use traceable weight sets prior to and after each time we use the balance. The traceable weight sets are also calibrated as required. Once you order them they come with a recalibration date and then its yearly after that. If the scale or balance is not within limits and the variation cannot be corrected then you would need to retire the instrument and purchase a new one. If the instrument can be adjusted then the calibration report would list that the instrument "as found" did not pass calibration but was adjusted and then was calibrated "as left". For us in a GLP environment if a particular piece of equipment is found to be out of tolerance we need to document that and also determine if it had any effect on any GLP study that that piece of equipment was used on. If you use your balance or scale a lot then you may consider a twice a year calibration. For us we calibrate the majority of our instruments yearly except for our pipettors which we calibrate quarterly. I hope this helps Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Wednesday, June 18, 2014 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Balance and Scale Calibration Hi Everyone, I was wondering what other anatomic pathology labs are doing to calibrate their scales and balances? I also would like to know what the basic tolerances for balances or scales is? I can only seem to find information in laboratories that deal with soil. I have a procedure and a calibration record sheet in place but I'm not sure what the range is for basic tolerances of the scale or balance so I'm not sure what to do if a scale or balance is not within the right limits. Thank you for any insight, procedures, information etc. Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Matthew.Lauterbach <@t> avera.org Wed Jun 18 12:54:37 2014 From: Matthew.Lauterbach <@t> avera.org (Matthew Lauterbach) Date: Wed Jun 18 12:54:46 2014 Subject: [Histonet] Full Time Day Shift HT/HTL Position in Sioux Falls, South Dakota Message-ID: <0FFD9B96579AA64D9A452478AFB414E2020A751B@AHDC374ML4.averamail.net> http://www.avera.org/mckennan/jobs/ Histology Technician -Req Num: 1402696 Category: Technical/Professional (Clinical) Facility: Avera McKennan Hospital & University Health Center Department: Lab Histology Schedule: Full Time Shift: Day Shift Hours: 5:00am-1:30pm, 5:30am-2:00pm, 7:30am-4:00pm, 8:30am-5:00pm Requisition: 1402696 Job Details: SUMMARY: The Histology Technician performs standardized histotechnology procedures under supervision by the Histology Section Supervisor and Section Pathologist. EDUCATION and/or EXPERIENCE: * An Associate's degree in Histology is preferred. CERTIFICATION, LICENSURE, and/or REGISTRATIONS: * Certification as a Histology Technician (HT) from the American Society Clinical Pathology (ASCP) certification is required or must be received within one year of employment or the completion of a National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) accredited Histotechnician program, whichever occurs last. Matthew J. Lauterbach, MBA, MLS (ASCP)CM Laboratory Services Manager Phlebotomy/Campus Services/Histology/POC Avera McKennan Region Phone: (605) 322-7126 Cell: (605) 496-1272 Fax: (605) 322-7083 From TNMayer <@t> mdanderson.org Wed Jun 18 14:20:30 2014 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Jun 18 14:21:35 2014 Subject: [Histonet] RE: control slides Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B3B4854@D1PWPEXMBX05.mdanderson.edu> Juan, If you don't need them soon, I might be able to help you. We are going to be clearing out some of our slides and taking pictures of them. After I check with my PD I will see if we can send you some. You can contact me offline at: tnmayer@mdanderson.org I will get back with you next week sometime and let you know. Sincerely, Toysha N. Mayer, MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 4 Date: Wed, 18 Jun 2014 16:24:17 +0000 From: "Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US)" Subject: [Histonet] control slides To: "Histonet@lists.utsouthwestern.edu" Message-ID: <5BEFD2C58946394F8E2A6EC3E1F86A9B15A31644@umechpanz.easf.csd.disa.mil> Content-Type: text/plain; charset="us-ascii" I am in need of control slides (already stained) of different type special stains for teaching purposes. H & E slides also needed. If you are in the Maryland area I may be able to pick up from your area. If out of state I'll pick up shipping . Juan l. Bassett HT Histotechnician Anatomical Div. National Museum of Health and Medicine 2500 Linden Lane Silver Spring, MD 20910 Office 301-319-3359 Lab 301-319-3328 Mobile 240-505-7247 Juan.l.bassett.ctr@mail.mil CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 24 ***************************************** From acoscetti <@t> gmail.com Wed Jun 18 22:02:55 2014 From: acoscetti <@t> gmail.com (Amanda Coscetti) Date: Wed Jun 18 22:03:07 2014 Subject: [Histonet] Liver bxs References: <24FC8F04-D3B6-4CDE-AC2C-E15DE35F9399@gmail.com> Message-ID: <29095B14-B76E-43C2-842E-A0C83324D11D@gmail.com> > > Hi HistoWorld! > Wondering if anyone has seen/heard of this as it has happened to us a couple of times in the past year and just happened again today! > We had a liver bx come from Radiology and we processed it as usual but under the microscope our pathologist said it was unreadable, the edges had artifact, and it had inconsistent staining (on the H&E, iron, and trichrome slides). The radiologist claims he put the specimen directly into formalin. All of our other bxs and tissue were fine. This was the case before when it happened and we have only had this problem with liver bxs! > Any ideas why this may occur? > > Thanks y'all! > Amanda > Florence SC > From Tony.Reilly <@t> health.qld.gov.au Wed Jun 18 23:10:21 2014 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Wed Jun 18 23:10:49 2014 Subject: [Histonet] RE: VIP 1000 In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF306980C1@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF306980C1@CHIEX005.CHI.catholichealth.net> Message-ID: Hi Tanya A number of years ago I had a fume hood which had very expensive disposable filters. I approached the company that serviced and calibrated our fume extraction equipment and they were able to manufacture permanent filter cartridges to suit our needs. From then on at a much lower price we purchased activated carbon to replace and refill the filters as required. Not quite the same problem but could be an alternative fix. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _________________________________________________ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Thursday, 19 June 2014 1:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VIP 1000 To go along with my last question, does anyone know where I could get charcoal filters for the VIP 1000 tissue processor? I have a letter from 2002 from Sakura saying parts for this will probably only be available for 2 more years, so that's only 10 years ago,..........!! Assuming if I can't get filters, I can't run it anyway?! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From mpence <@t> grhs.net Thu Jun 19 08:03:01 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jun 19 08:03:23 2014 Subject: [Histonet] Liver bxs In-Reply-To: <29095B14-B76E-43C2-842E-A0C83324D11D@gmail.com> References: <24FC8F04-D3B6-4CDE-AC2C-E15DE35F9399@gmail.com> <29095B14-B76E-43C2-842E-A0C83324D11D@gmail.com> Message-ID: Ask to be present at the next liver bx time and see for yourself their handling of the specimen. Sounds like it set out for some time. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Coscetti Sent: Wednesday, June 18, 2014 10:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Liver bxs > > Hi HistoWorld! > Wondering if anyone has seen/heard of this as it has happened to us a couple of times in the past year and just happened again today! > We had a liver bx come from Radiology and we processed it as usual but under the microscope our pathologist said it was unreadable, the edges had artifact, and it had inconsistent staining (on the H&E, iron, and trichrome slides). The radiologist claims he put the specimen directly into formalin. All of our other bxs and tissue were fine. This was the case before when it happened and we have only had this problem with liver bxs! > Any ideas why this may occur? > > Thanks y'all! > Amanda > Florence SC > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Breckenridge <@t> uth.tmc.edu Thu Jun 19 08:22:16 2014 From: Richard.Breckenridge <@t> uth.tmc.edu (Breckenridge, Richard A) Date: Thu Jun 19 08:22:39 2014 Subject: [Histonet] Anti-PLA2r Message-ID: <226B9BF4FE89CB4D96DEC62F59DF97F1030B79@UTHMAIL1.uthouston.edu> Hello all, We are revisiting the Anti-PLA2r fluorescence staining for renal biopsies. I am in need of a working protocol. I need to know of a good antibody to use. We have unsuccessfully tried using a couple of antibodies now and it is getting expensive. Any help would be appreciated. Thanks. Best regards, Richard A. Breckenridge, HT(ASCP) Special Tissues Laboratory Manager University of Texas Health Science Center at Houston Medical School Building 2.002 ph. 713-500-6792 fax 713-500-0733 richard.breckenridge@uth.tmc.edu From gayle.callis <@t> bresnan.net Thu Jun 19 10:01:36 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Jun 19 10:01:55 2014 Subject: [Histonet] Re: VIP 1000 charcoal filters Message-ID: <000001cf8bcf$5fae66f0$1f0b34d0$@bresnan.net> Years ago, we went to a pet store (Petco, Petsmart) or pet section in Walmart, etc. for bags of bulk charcoal used in fish tank filters. I am sure Amazon will have this too. The charcoal pieces were very similar to what was in prefilled VIP filters. We emptied the VIP charcoal containers under a hood to avoid xylene fumes captured by charcoal then refilled with the fish tank filter charcoal. If you try this, note the level of charcoal in the boxes for quantity needed. It was actually cheaper than buying prefilled boxes from the vendor's, and no shipping costs either. Unfortunately, Sakura discontinued charcoal "milk carton" packaging, probably to avoid the fume problem during filter change - a safety factor. This is somewhat of a stop gap measure but certainly worked for us. The more recent charcoal boxes are cardboard and sealed, if I remember correctly, but careful opening and then resealing with duct tape might work. However, first VIP charcoal filter containers were made of the same plastic as the solvent containers and not easily damaged during a change. Good luck Gayle Callis HTL/HT/MT(ASCP) From renafail2 <@t> gmail.com Thu Jun 19 11:20:49 2014 From: renafail2 <@t> gmail.com (Rena Fail) Date: Thu Jun 19 11:21:01 2014 Subject: [Histonet] Liver bxs In-Reply-To: References: <24FC8F04-D3B6-4CDE-AC2C-E15DE35F9399@gmail.com> <29095B14-B76E-43C2-842E-A0C83324D11D@gmail.com> Message-ID: Your description is consistent with the tissue having dried out. So observing their procedure may be helpful. Is it placed on gauze then placed in formalin ? That would dry it quickly. are they tending to the pt then placing the specimen in fprmalin, Rena Fail On Thu, Jun 19, 2014 at 9:03 AM, Mike Pence wrote: > Ask to be present at the next liver bx time and see for yourself their > handling of the specimen. Sounds like it set out for some time. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Coscetti > Sent: Wednesday, June 18, 2014 10:03 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Liver bxs > > > > > > Hi HistoWorld! > > Wondering if anyone has seen/heard of this as it has happened to us a > couple of times in the past year and just happened again today! > > We had a liver bx come from Radiology and we processed it as usual but > under the microscope our pathologist said it was unreadable, the edges had > artifact, and it had inconsistent staining (on the H&E, iron, and trichrome > slides). The radiologist claims he put the specimen directly into > formalin. All of our other bxs and tissue were fine. This was the case > before when it happened and we have only had this problem with liver bxs! > > Any ideas why this may occur? > > > > Thanks y'all! > > Amanda > > Florence SC > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tissuetech <@t> juno.com Thu Jun 19 16:17:36 2014 From: tissuetech <@t> juno.com (tissuetech@juno.com) Date: Thu Jun 19 16:19:14 2014 Subject: [Histonet] Billing Programs Message-ID: <20140619.161736.17854.0@webmail03.vgs.untd.com> Hi All I have a question that I hope someone maybe able to help with: We presently use a billing software program known as EZ Billing and find it to be very complicated. I would like to hear from others about what you are using and why, we are looking for options to our present system. EZ Billing is coupled with AP Easy (our base system) which works well, and would like to find another Billing program that will interface with AP Easy which we have customized over several years to fit our operation. I am hoping that there is someone out in the Histo world that can provide assistance. Please reply to me off line; my office number is 972.241-6277, e-mail: fsiena@tissuetechpathology.com I thank you in advance for your time and assistance in this matter. Respectfully; Fred Siena Tissue Techniques Pathology Labs ____________________________________________________________ 1 Odd trick Kills diabetes 100% scientifically-proven way to control blood sugar in 3 short weeks http://thirdpartyoffers.juno.com/TGL3141/53a353941576053935483st01vuc From rennie1108 <@t> yahoo.com Thu Jun 19 16:24:24 2014 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Thu Jun 19 16:24:34 2014 Subject: [Histonet] Eosin Leaching Message-ID: Hello all, We?re having a problem with our eosin bleeding out of the sections. I?ve read that this can be caused by not dehydrating adequately after eosin, but we?re still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. From jqb7 <@t> cdc.gov Thu Jun 19 16:26:03 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu Jun 19 16:26:19 2014 Subject: [Histonet] Eosin Leaching In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B7811FD2B3EE@EMBX-CHAM2.cdc.gov> Have you changed your coverslipping mountant? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina.kreutzer01 <@t> gmail.com Fri Jun 20 07:08:24 2014 From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer) Date: Fri Jun 20 07:08:33 2014 Subject: [Histonet] open source NIS ELEMENTS Message-ID: Hello, we have a Nikon Eclipse E600 with a DS 2Mv camera, it was a present from another lab and the dongle for the Software is missing. So before I am contacting Nikon if it is possible to get a free/cheap additional dongle, I wanted to ask you if somebody knows a capable open source equivalent to the NIS ELEMENTS softare. Regards Christina From Toni.Rathborne <@t> rwjuh.edu Fri Jun 20 07:11:02 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Fri Jun 20 07:11:08 2014 Subject: [Histonet] Eosin Leaching In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FD2B3EE@EMBX-CHAM2.cdc.gov> References: <3B2CD438E1628A41BD687E98B963B7811FD2B3EE@EMBX-CHAM2.cdc.gov> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24E5EF85@VAP1013.win.rwjuh.edu> Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rennie1108 <@t> yahoo.com Fri Jun 20 07:15:02 2014 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Fri Jun 20 07:15:08 2014 Subject: [Histonet] Eosin Leaching In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24E5EF85@VAP1013.win.rwjuh.edu> References: <3B2CD438E1628A41BD687E98B963B7811FD2B3EE@EMBX-CHAM2.cdc.gov> <59E09A4EFBD3F349BD75FDAE8AFB0F24E5EF85@VAP1013.win.rwjuh.edu> Message-ID: We haven?t changed our mounting medium, but I?ll have to check on the alcohol levels. When I stain (we hand stain only - very small lab), I always make sure the alcohol is higher than the eosin, but I have a lab assistant now, and it?s very possible I didn?t make that a point. I?ll try that. Any more tips are welcome still - thank you all! Adrienne On Jun 20, 2014, at 8:11 AM, Rathborne, Toni wrote: > Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) > Sent: Thursday, June 19, 2014 5:26 PM > To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' > Subject: RE: [Histonet] Eosin Leaching > > Have you changed your coverslipping mountant? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson > Sent: Thursday, June 19, 2014 5:24 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Eosin Leaching > > Hello all, > > We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? > > Thanks in advance for your help! > > Adrienne > > > > Adrienne Anderson, BS, HTL(ASCP) > Histotechnologist > Phylogeny, Inc. > 1476 Manning Pkwy, Powell, Ohio 43605 > Phone: (614) 846-6161 > Fax: (877) 591-1815 > > This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. > Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. > If you are not the intended recipient, please notify the sender and delete this message from your system. > Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From abuchiane <@t> bmhvt.org Fri Jun 20 08:40:18 2014 From: abuchiane <@t> bmhvt.org (Anita Buchiane) Date: Fri Jun 20 08:40:39 2014 Subject: [Histonet] Eosin Leaching In-Reply-To: References: <3B2CD438E1628A41BD687E98B963B7811FD2B3EE@EMBX-CHAM2.cdc.gov> <59E09A4EFBD3F349BD75FDAE8AFB0F24E5EF85@VAP1013.win.rwjuh.edu> Message-ID: <4034E71604330C4B8E10D1538DFB2455EC6A1316@BMHEXCH02.bmhvt.org> I'd advise checking your dehydration alcohols with a hydrometer. This "bleeding" happened to me once and it turned out the alcohols after the Eosin were not graded. My lab aide accidently used 100% only instead of 70%, 95% and then 100%. Anita Anita -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Friday, June 20, 2014 8:15 AM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Eosin Leaching We haven't changed our mounting medium, but I'll have to check on the alcohol levels. When I stain (we hand stain only - very small lab), I always make sure the alcohol is higher than the eosin, but I have a lab assistant now, and it's very possible I didn't make that a point. I'll try that. Any more tips are welcome still - thank you all! Adrienne On Jun 20, 2014, at 8:11 AM, Rathborne, Toni wrote: > Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) > Sent: Thursday, June 19, 2014 5:26 PM > To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' > Subject: RE: [Histonet] Eosin Leaching > > Have you changed your coverslipping mountant? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson > Sent: Thursday, June 19, 2014 5:24 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Eosin Leaching > > Hello all, > > We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? > > Thanks in advance for your help! > > Adrienne > > > > Adrienne Anderson, BS, HTL(ASCP) > Histotechnologist > Phylogeny, Inc. > 1476 Manning Pkwy, Powell, Ohio 43605 > Phone: (614) 846-6161 > Fax: (877) 591-1815 > > This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. > Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. > If you are not the intended recipient, please notify the sender and delete this message from your system. > Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. 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The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ From Richard.Cartun <@t> hhchealth.org Fri Jun 20 08:43:52 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Jun 20 08:44:58 2014 Subject: [Histonet] EM question Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E1C2319DC@HHCEXCHMB03.hhcsystem.org> I have a question for those labs still performing Electron Microscopy for diagnosis. What do you do if you have a specimen that turns out to be "unsatisfactory" for evaluation after you have done all the prep work? Can you bill an 88348 (technical charge) without billing the professional fee (88348-26)? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From vavalos <@t> allergydermatology.com Fri Jun 20 09:37:36 2014 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Fri Jun 20 09:37:45 2014 Subject: [Histonet] Eosin Leaching In-Reply-To: References: Message-ID: <2b14610f041b4b88812538860fb81d30@BY2PR07MB217.namprd07.prod.outlook.com> We had the same problem. Increase your Alcohol/Reagents (following Eosin) times as well as using fresh ones. I use 80% for about 20 sec and (2)100% for a minute each and (3) Xylene Subs for 2 min each. I also use a fresh 80% and rotate the 100% each day. If using a Xylene Sub it could also be a possibility that your mounting medium is not compatible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bruce_Palmatier <@t> vwr.com Fri Jun 20 14:06:00 2014 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Fri Jun 20 14:06:24 2014 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 06/23/2014) Message-ID: I am out of the office until 06/23/2014. I will be traveling on June 20th and will have limited access to email. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 127, Issue 25" sent on 6/20/2014 10:54:03 AM. This is the only notification you will receive while this person is away. From bfoster <@t> mme1.com Fri Jun 20 15:06:48 2014 From: bfoster <@t> mme1.com (Barbara Foster) Date: Fri Jun 20 15:07:12 2014 Subject: [Histonet] Fwd: Histonet Digest, Vol 127, Issue 25 - Control slides Message-ID: <201406202007.s5KK72Qw006247@atl4mhob01.myregisteredsite.com> There is new technology available now for standardizing color images so, especially if you are taking images of control slides of stained sections, consider using it. On the acquisition side, it standardizes the system and camera response using a calibration slide with a matrix of known colors. The software also auto adjusts brightness and white balance, resulting in highly consistent, reproducible results. On the display side, the kit has an easy to use colorimeter which provides empirical calibration of the monitor (not just an electronic look up table). Called ChromaCal (chromacal.com), it will be on display this next week at Soc. for Tox Path (STP, Washington DC). Hope this is helpful, Barbara Foster Caveat: No commerical interest >Delivered-To: bfoster@1535597.1652944 >Date: Thu, 19 Jun 2014 13:02:40 -0400 >From: histonet-request@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 127, Issue 25 >To: histonet@lists.utsouthwestern.edu >Reply-To: histonet@lists.utsouthwestern.edu >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: b81d4fd83ad9df6380da27b3c47251c0 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false >X-SpamScore: 1.5 >X-MailHub-Apparently-To: bfoster@mme1.com > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. RE: Balance and Scale Calibration (Elizabeth Chlipala) > 2. Full Time Day Shift HT/HTL Position in Sioux Falls, South > Dakota (Matthew Lauterbach) > 3. RE: control slides (Mayer,Toysha N) > 4. Liver bxs (Amanda Coscetti) > 5. RE: VIP 1000 (Tony Reilly) > 6. RE: Liver bxs (Mike Pence) > 7. Anti-PLA2r (Breckenridge, Richard A) > 8. Re: VIP 1000 charcoal filters (gayle callis) > 9. Re: Liver bxs (Rena Fail) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Wed, 18 Jun 2014 11:02:52 -0600 >From: Elizabeth Chlipala >Subject: [Histonet] RE: Balance and Scale Calibration >To: "Piche, Jessica" , > "histonet@lists.utsouthwestern.edu" > >Message-ID: > ><14E2C6176416974295479C64A11CB9AE019C79ECD156@SBS2K8.premierlab.local> >Content-Type: text/plain; charset="us-ascii" > >Jessica > >Your operating manual for your balance should list your level for >tolerance and any good calibration company will also know what is >acceptable, this tolerance level can also be governed by your SOP >too. We have a Mettler Toledo Model AB204-S that measures out to >0.0000 the tolerance on our calibration report is +/- 0.0005. We >are not a clinical lab but we have our balance calibrated once a >year and we also use traceable weight sets prior to and after each >time we use the balance. The traceable weight sets are also >calibrated as required. Once you order them they come with a >recalibration date and then its yearly after that. If the scale or >balance is not within limits and the variation cannot be corrected >then you would need to retire the instrument and purchase a new >one. If the instrument can be adjusted then the calibration report >would list that the instrument "as found" did not pass calibration >but was adjusted and then was calibrated "as left". For us in a GLP >environment if a particular piece of equipment is found to be out of >tolerance we need to document that and also determine if it had any >effect on any GLP study that that piece of equipment was used >on. If you use your balance or scale a lot then you may consider a >twice a year calibration. For us we calibrate the majority of our >instruments yearly except for our pipettors which we calibrate quarterly. > >I hope this helps > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Premier Laboratory, LLC >PO Box 18592 >Boulder, CO 80308 >(303) 682-3949 office >(303) 682-9060 fax >(303) 881-0763 cell >liz@premierlab.com >www.premierlab.com > >March 10, 2014 is Histotechnology Professionals Day > >Ship to Address: > >Premier Laboratory, LLC >1567 Skyway Drive, Unit E >Longmont, CO 80504 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica >Sent: Wednesday, June 18, 2014 10:37 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Balance and Scale Calibration > >Hi Everyone, > >I was wondering what other anatomic pathology labs are doing to >calibrate their scales and balances? I also would like to know what >the basic tolerances for balances or scales is? I can only seem to >find information in laboratories that deal with soil. I have a >procedure and a calibration record sheet in place but I'm not sure >what the range is for basic tolerances of the scale or balance so >I'm not sure what to do if a scale or balance is not within the right limits. > >Thank you for any insight, procedures, information etc. > >Jessica Piche, HT(ASCP) >Waterbury Hospital > > > > >CONFIDENTIALITY NOTICE: This email and any attachments contain >confidential information that is legally privileged. This >information is intended only for the use of the individual or entity >named above. The authorized recipient of this information is >prohibited from disclosing this information to any other party >unless required to do so by law or regulation. If you are not the >intended recipient, you are hereby notified that any disclosure, >copying, distribution or action taken in reliance on the contents of >these documents is strictly prohibited. If you have received this >information in error, please notify the sender immediately and >delete these documents. Copyright (c) Waterbury Hospital >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 2 >Date: Wed, 18 Jun 2014 17:54:37 +0000 >From: Matthew Lauterbach >Subject: [Histonet] Full Time Day Shift HT/HTL Position in Sioux > Falls, South Dakota >To: "'Histonet@lists.utsouthwestern.edu'" > >Message-ID: > <0FFD9B96579AA64D9A452478AFB414E2020A751B@AHDC374ML4.averamail.net> >Content-Type: text/plain; charset="us-ascii" > >http://www.avera.org/mckennan/jobs/ > >Histology Technician -Req Num: 1402696 >Category: > Technical/Professional (Clinical) >Facility: > Avera McKennan Hospital & University Health Center >Department: > Lab Histology >Schedule: > Full Time >Shift: > Day Shift >Hours: > 5:00am-1:30pm, 5:30am-2:00pm, 7:30am-4:00pm, 8:30am-5:00pm >Requisition: > 1402696 >Job Details: > SUMMARY: The Histology Technician performs standardized > histotechnology procedures under supervision by the Histology > Section Supervisor and Section Pathologist. > >EDUCATION and/or EXPERIENCE: > * An Associate's degree in Histology is preferred. > >CERTIFICATION, LICENSURE, and/or REGISTRATIONS: > * Certification as a Histology Technician (HT) from the > American Society Clinical Pathology (ASCP) certification is > required or must be received within one year of employment or > the completion of a National Accrediting Agency for Clinical > Laboratory Sciences (NAACLS) accredited Histotechnician program, > whichever occurs last. > > >Matthew J. Lauterbach, MBA, MLS (ASCP)CM >Laboratory Services Manager >Phlebotomy/Campus Services/Histology/POC >Avera McKennan Region >Phone: (605) 322-7126 >Cell: (605) 496-1272 >Fax: (605) 322-7083 > > > > >------------------------------ > >Message: 3 >Date: Wed, 18 Jun 2014 19:20:30 +0000 >From: "Mayer,Toysha N" >Subject: [Histonet] RE: control slides >To: "'histonet@lists.utsouthwestern.edu'" > >Message-ID: > ><47E9B2C01DDDD94881EACD2DC44EBC881B3B4854@D1PWPEXMBX05.mdanderson.edu> >Content-Type: text/plain; charset="us-ascii" > >Juan, > >If you don't need them soon, I might be able to help you. We are >going to be clearing out some of our slides and taking pictures of them. >After I check with my PD I will see if we can send you some. >You can contact me offline at: tnmayer@mdanderson.org >I will get back with you next week sometime and let you know. > >Sincerely, > >Toysha N. Mayer, MBA, HT (ASCP) >Instructor/Education Coordinator >Program in Histotechnology >School of Health Professions >UT M.D. Anderson Cancer Center >713.563-3481 > >This electronic mail and any attached documents are intended solely >for the named addressee(s) and contain confidential information. If >you are not an addressee, or responsible for delivering this email >to an addressee, you have received this email in error and are >notified that reading, copying, or disclosing this email is >prohibited. If you received this email in error, immediately reply >to the sender and delete the message completely from your computer system. > > >------------------------------ > >Message: 4 >Date: Wed, 18 Jun 2014 16:24:17 +0000 >From: "Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US)" > >Subject: [Histonet] control slides >To: "Histonet@lists.utsouthwestern.edu" > >Message-ID: > ><5BEFD2C58946394F8E2A6EC3E1F86A9B15A31644@umechpanz.easf.csd.disa.mil> >Content-Type: text/plain; charset="us-ascii" > >I am in need of control slides (already stained) of different type >special stains for teaching purposes. H & E slides also needed. If >you are in the Maryland area I may be able to pick up from your >area. If out of state I'll pick up shipping . > >Juan l. Bassett HT >Histotechnician Anatomical Div. >National Museum of Health and Medicine >2500 Linden Lane >Silver Spring, MD 20910 >Office 301-319-3359 >Lab 301-319-3328 >Mobile 240-505-7247 > >Juan.l.bassett.ctr@mail.mil > > > > > > > >CONFIDENTIALITY NOTICE: This email and any attachments contain >confidential information that is legally privileged. This >information is intended only for the use of the individual or entity >named above. The authorized recipient of this information is >prohibited from disclosing this information to any other party >unless required to do so by law or regulation. If you are not the >intended recipient, you are hereby notified that any disclosure, >copying, distribution or action taken in reliance on the contents of >these documents is strictly prohibited. If you have received this >information in error, please notify the sender immediately and >delete these documents. Copyright (c) Waterbury Hospital > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 127, Issue 24 >***************************************** > > > >------------------------------ > >Message: 4 >Date: Wed, 18 Jun 2014 23:02:55 -0400 >From: Amanda Coscetti >Subject: [Histonet] Liver bxs >To: "histonet@lists.utsouthwestern.edu" > >Message-ID: <29095B14-B76E-43C2-842E-A0C83324D11D@gmail.com> >Content-Type: text/plain; charset=us-ascii > > > > > > Hi HistoWorld! > > Wondering if anyone has seen/heard of this as it has happened to > us a couple of times in the past year and just happened again today! > > We had a liver bx come from Radiology and we processed it as > usual but under the microscope our pathologist said it was > unreadable, the edges had artifact, and it had inconsistent > staining (on the H&E, iron, and trichrome slides). The > radiologist claims he put the specimen directly into formalin. All > of our other bxs and tissue were fine. This was the case before > when it happened and we have only had this problem with liver bxs! > > Any ideas why this may occur? > > > > Thanks y'all! > > Amanda > > Florence SC > > > > > > > > > > >------------------------------ > >Message: 5 >Date: Thu, 19 Jun 2014 14:10:21 +1000 >From: Tony Reilly >Subject: [Histonet] RE: VIP 1000 >To: "Abbott, Tanya" , > "histonet@lists.utsouthwestern.edu" > >Message-ID: > > > >Content-Type: text/plain; charset="iso-8859-1" > >Hi Tanya > >A number of years ago I had a fume hood which had very expensive >disposable filters. I approached the company that serviced and >calibrated our fume extraction equipment and they were able to >manufacture permanent filter cartridges to suit our needs. From >then on at a much lower price we purchased activated carbon to >replace and refill the filters as required. Not quite the same >problem but could be an alternative fix. > >Regards >Tony > > >Tony Reilly B.App.Sc, M.Sc >Chief Scientist >Anatomical Pathology >Pathology Queensland PAH >_________________________________________________ >Health Services Support Agency| Department of Health > >Building 15, Level 1, >199 Ipswich Road >WOOLLOONGABBA Queensland 4102 >Ph: 07 3176 2412 >Mob: 0402139411 >Fax: 07 3176 2930 >Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au > > > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya >Sent: Thursday, 19 June 2014 1:57 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] VIP 1000 > >To go along with my last question, does anyone know where I could >get charcoal filters for the VIP 1000 tissue processor? I have a >letter from 2002 from Sakura saying parts for this will probably >only be available for 2 more years, so that's only 10 years >ago,..........!! Assuming if I can't get filters, I can't run it anyway?! > >Tanya G. Abbott RT (CSMLS) >Manager Technologist, Histology/Cytology St. Joseph Medical Center >Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 >email: tanyaabbott@catholichealth.net > >This electronic mail and any attached documents are intended solely >for the named addressee(s) and contain confidential information. If >you are not an addressee, or responsible for delivering this email >to an addressee, you have received this email in error and are >notified that reading, copying, or disclosing this email is >prohibited. If you received this email in error, immediately reply >to the sender and delete the message completely from your computer system. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >******************************************************************************** >This email, including any attachments sent with it, is confidential >and for the sole use of the intended recipient(s). This >confidentiality is not waived or lost, if you receive it and you are >not the intended recipient(s), or if it is transmitted/received in error. >Any unauthorised use, alteration, disclosure, distribution or review >of this email is strictly prohibited. The information contained in >this email, including any attachment sent with it, may be subject to >a statutory duty of confidentiality if it relates to health service matters. >If you are not the intended recipient(s), or if you have received >this email in error, you are asked to immediately notify the sender >by telephone collect on Australia +61 1800 198 175 or by return >email. You should also delete this email, and any copies, from your >computer system network and destroy any hard copies produced. >If not an intended recipient of this email, you must not copy, >distribute or take any action(s) that relies on it; any form of >disclosure, modification, distribution and/or publication of this >email is also prohibited. >Although Queensland Health takes all reasonable steps to ensure this >email does not contain malicious software, Queensland Health does >not accept responsibility for the consequences if any person's >computer inadvertently suffers any disruption to services, loss of >information, harm or is infected with a virus, other malicious >computer programme or code that may occur as a consequence of >receiving this email. >Unless stated otherwise, this email represents only the views of the >sender and not the views of the Queensland Government. >********************************************************************************** > > > > >------------------------------ > >Message: 6 >Date: Thu, 19 Jun 2014 13:03:01 +0000 >From: Mike Pence >Subject: RE: [Histonet] Liver bxs >To: 'Amanda Coscetti' , > "histonet@lists.utsouthwestern.edu" > >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >Ask to be present at the next liver bx time and see for yourself >their handling of the specimen. Sounds like it set out for some time. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Coscetti >Sent: Wednesday, June 18, 2014 10:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Liver bxs > > > > > > Hi HistoWorld! > > Wondering if anyone has seen/heard of this as it has happened to > us a couple of times in the past year and just happened again today! > > We had a liver bx come from Radiology and we processed it as > usual but under the microscope our pathologist said it was > unreadable, the edges had artifact, and it had inconsistent > staining (on the H&E, iron, and trichrome slides). The > radiologist claims he put the specimen directly into formalin. All > of our other bxs and tissue were fine. This was the case before > when it happened and we have only had this problem with liver bxs! > > Any ideas why this may occur? > > > > Thanks y'all! > > Amanda > > Florence SC > > > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >------------------------------ > >Message: 7 >Date: Thu, 19 Jun 2014 13:22:16 +0000 >From: "Breckenridge, Richard A" >Subject: [Histonet] Anti-PLA2r >To: "histonet@lists.utsouthwestern.edu" > >Message-ID: > <226B9BF4FE89CB4D96DEC62F59DF97F1030B79@UTHMAIL1.uthouston.edu> >Content-Type: text/plain; charset="us-ascii" > >Hello all, >We are revisiting the Anti-PLA2r fluorescence staining for renal >biopsies. I am in need of a working protocol. I need to know of a >good antibody to use. We have unsuccessfully tried using a couple of >antibodies now and it is getting expensive. Any help would be >appreciated. Thanks. > >Best regards, > >Richard A. Breckenridge, HT(ASCP) >Special Tissues Laboratory Manager >University of Texas Health Science Center at Houston >Medical School Building 2.002 >ph. 713-500-6792 fax 713-500-0733 >richard.breckenridge@uth.tmc.edu > > > >------------------------------ > >Message: 8 >Date: Thu, 19 Jun 2014 09:01:36 -0600 >From: "gayle callis" >Subject: [Histonet] Re: VIP 1000 charcoal filters >To: "'Histonet '" >Message-ID: <000001cf8bcf$5fae66f0$1f0b34d0$@bresnan.net> >Content-Type: text/plain; charset="us-ascii" > >Years ago, we went to a pet store (Petco, Petsmart) or pet section in >Walmart, etc. for bags of bulk charcoal used in fish tank filters. I am sure >Amazon will have this too. The charcoal pieces were very similar to what >was in prefilled VIP filters. We emptied the VIP charcoal containers >under a hood to avoid xylene fumes captured by charcoal then refilled with >the fish tank filter charcoal. If you try this, note the level of charcoal >in the boxes for quantity needed. It was actually cheaper than buying >prefilled boxes from the vendor's, and no shipping costs either. >Unfortunately, Sakura discontinued charcoal "milk carton" packaging, >probably to avoid the fume problem during filter change - a safety factor. > >This is somewhat of a stop gap measure but certainly worked for us. The >more recent charcoal boxes are cardboard and sealed, if I remember >correctly, but careful opening and then resealing with duct tape might work. >However, first VIP charcoal filter containers were made of the same plastic >as the solvent containers and not easily damaged during a change. > > > >Good luck > > > >Gayle Callis > >HTL/HT/MT(ASCP) > > > > > > > > > >------------------------------ > >Message: 9 >Date: Thu, 19 Jun 2014 12:20:49 -0400 >From: Rena Fail >Subject: Re: [Histonet] Liver bxs >To: Mike Pence >Cc: "histonet@lists.utsouthwestern.edu" > >Message-ID: > >Content-Type: text/plain; charset=UTF-8 > > Your description is consistent with the tissue having dried out. So >observing their procedure may be helpful. Is it placed on gauze then placed >in formalin ? That would dry it quickly. are they tending to the pt then >placing the specimen in fprmalin, >Rena Fail > > >On Thu, Jun 19, 2014 at 9:03 AM, Mike Pence wrote: > > > Ask to be present at the next liver bx time and see for yourself their > > handling of the specimen. Sounds like it set out for some time. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Coscetti > > Sent: Wednesday, June 18, 2014 10:03 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Liver bxs > > > > > > > > > > Hi HistoWorld! > > > Wondering if anyone has seen/heard of this as it has happened to us a > > couple of times in the past year and just happened again today! > > > We had a liver bx come from Radiology and we processed it as usual but > > under the microscope our pathologist said it was unreadable, the edges had > > artifact, and it had inconsistent staining (on the H&E, iron, and trichrome > > slides). The radiologist claims he put the specimen directly into > > formalin. All of our other bxs and tissue were fine. This was the case > > before when it happened and we have only had this problem with liver bxs! > > > Any ideas why this may occur? > > > > > > Thanks y'all! > > > Amanda > > > Florence SC > > > > > > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 127, Issue 25 >***************************************** From Tony.Reilly <@t> health.qld.gov.au Sun Jun 22 18:22:28 2014 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Sun Jun 22 18:23:29 2014 Subject: [Histonet] Eosin Leaching In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24E5EF85@VAP1013.win.rwjuh.edu> References: <3B2CD438E1628A41BD687E98B963B7811FD2B3EE@EMBX-CHAM2.cdc.gov> <59E09A4EFBD3F349BD75FDAE8AFB0F24E5EF85@VAP1013.win.rwjuh.edu> Message-ID: Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _________________________________________________ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histonet@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From Lesley.Bechtold <@t> jax.org Mon Jun 23 10:25:27 2014 From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold) Date: Mon Jun 23 10:25:38 2014 Subject: [Histonet] Histology Benchmarking Survey Message-ID: <08997064E2075247A4DD5A035DB51CDF64FA0094@jaxbhexms02.jax.org> Dear Histonetters, We are seeking information from our peers in the Histology world. To better understand what other Histology Labs offer, we have created a short survey that we hope you will fill out. The link to the survey is below. Information is a useful tool when preparing budgets, hiring staff, buying equipment or making the decision to add new processes to your lab. Anyone who fills out the survey will receive a copy of the de-identified results. We thank you in advance for participating. If surveys annoy you, please delete this email. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) https://www.surveymonkey.com/s/HistoSurvey2014 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From Lori.Amos <@t> vdh.virginia.gov Mon Jun 23 12:29:24 2014 From: Lori.Amos <@t> vdh.virginia.gov (Amos, Lori (VDH)) Date: Mon Jun 23 12:30:12 2014 Subject: [Histonet] RE: Eosin Leaching In-Reply-To: <20d4df50-c314-43a1-a433-c162a4a13394@COVMSGCES-HUB02.cov.virginia.gov> References: <20d4df50-c314-43a1-a433-c162a4a13394@COVMSGCES-HUB02.cov.virginia.gov> Message-ID: <34AAD3B59AC6474EA3406AEC60C1F89B73C8A0@COVMSGCES-MBX12> I too have been experiencing Eosin leaching. I have tried all different options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols and xylenes that follow, even added a running water wash step after the Eosin ( to remove excess). Before this, I have had no problems and had made no changes to my daily activities. I rotate solutions daily. Aside from changing the company I buy my Eosin from, I am at a loss. Thanks in advance for your help. Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, June 23, 2014 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Eosin Leaching (Tony Reilly) 2. Histology Benchmarking Survey (Lesley Bechtold) ---------------------------------------------------------------------- Message: 1 Date: Mon, 23 Jun 2014 09:22:28 +1000 From: Tony Reilly Subject: RE: [Histonet] Eosin Leaching To: "Rathborne, Toni" , "Sanders, Jeanine (CDC/OID/NCEZID)" , "'Adrienne Anderson'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _________________________________________________ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histonet@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** ------------------------------ Message: 2 Date: Mon, 23 Jun 2014 15:25:27 +0000 From: Lesley Bechtold Subject: [Histonet] Histology Benchmarking Survey To: "histonet@lists.utsouthwestern.edu" Message-ID: <08997064E2075247A4DD5A035DB51CDF64FA0094@jaxbhexms02.jax.org> Content-Type: text/plain; charset="us-ascii" Dear Histonetters, We are seeking information from our peers in the Histology world. To better understand what other Histology Labs offer, we have created a short survey that we hope you will fill out. The link to the survey is below. Information is a useful tool when preparing budgets, hiring staff, buying equipment or making the decision to add new processes to your lab. Anyone who fills out the survey will receive a copy of the de-identified results. We thank you in advance for participating. If surveys annoy you, please delete this email. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) https://www.surveymonkey.com/s/HistoSurvey2014 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 28 ***************************************** From PAMarcum <@t> uams.edu Mon Jun 23 13:03:22 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Jun 23 13:03:32 2014 Subject: [Histonet] RE: Eosin Leaching In-Reply-To: <34AAD3B59AC6474EA3406AEC60C1F89B73C8A0@COVMSGCES-MBX12> References: <20d4df50-c314-43a1-a433-c162a4a13394@COVMSGCES-HUB02.cov.virginia.gov> <34AAD3B59AC6474EA3406AEC60C1F89B73C8A0@COVMSGCES-MBX12> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012403D1F2@Mail2Node2.ad.uams.edu> Have you changed your reagent alcohol or has the company you buy from changed where it purchases the reagent alcohol? Reagent alcohols with higher volumes of isopropanol than 5% can cause the eosin to leach out. It will leave a green powder in the bottom of you staining dish. Methanol and Isopropanol should be 5% each with 90% ethanol for the safest blend and what most of us old folks always thought reagent alcohol would stay. We were wrong and different companied can change vendors or sources and get something they were not expecting too. If you look up reagent alcohol or SDA reagent alcohol you find it has many formulas and the only real requirement by the ATF is to make the alcohol "non-drinkable or poison to humans". I have seen formulas with 5 to 8% methanol and isopropanol with as low as 87% ethanol. The percentage will depend on price as they have no idea at the manufacturing level what we use ti for or why. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos, Lori (VDH) Sent: Monday, June 23, 2014 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Eosin Leaching I too have been experiencing Eosin leaching. I have tried all different options: Adding acetic acid to the pre-made Eosin, adding time to the alcohols and xylenes that follow, even added a running water wash step after the Eosin ( to remove excess). Before this, I have had no problems and had made no changes to my daily activities. I rotate solutions daily. Aside from changing the company I buy my Eosin from, I am at a loss. Thanks in advance for your help. Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, June 23, 2014 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Eosin Leaching (Tony Reilly) 2. Histology Benchmarking Survey (Lesley Bechtold) ---------------------------------------------------------------------- Message: 1 Date: Mon, 23 Jun 2014 09:22:28 +1000 From: Tony Reilly Subject: RE: [Histonet] Eosin Leaching To: "Rathborne, Toni" , "Sanders, Jeanine (CDC/OID/NCEZID)" , "'Adrienne Anderson'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _________________________________________________ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histonet@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** ------------------------------ Message: 2 Date: Mon, 23 Jun 2014 15:25:27 +0000 From: Lesley Bechtold Subject: [Histonet] Histology Benchmarking Survey To: "histonet@lists.utsouthwestern.edu" Message-ID: <08997064E2075247A4DD5A035DB51CDF64FA0094@jaxbhexms02.jax.org> Content-Type: text/plain; charset="us-ascii" Dear Histonetters, We are seeking information from our peers in the Histology world. To better understand what other Histology Labs offer, we have created a short survey that we hope you will fill out. The link to the survey is below. Information is a useful tool when preparing budgets, hiring staff, buying equipment or making the decision to add new processes to your lab. Anyone who fills out the survey will receive a copy of the de-identified results. We thank you in advance for participating. If surveys annoy you, please delete this email. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) https://www.surveymonkey.com/s/HistoSurvey2014 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 28 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Jennifer.Johnson <@t> genzyme.com Mon Jun 23 13:06:55 2014 From: Jennifer.Johnson <@t> genzyme.com (Jennifer.Johnson@genzyme.com) Date: Mon Jun 23 13:07:08 2014 Subject: [Histonet] ISH preparation Message-ID: <0C0766AB928A0E4DB4760C0DB41F91B984A254CC@XSPW10A507T.pharma.aventis.com> Hi Folks! I was hoping to get a little input about preparing samples for ISH. We currently RNAse-away our tools at the time of collection, toss the tissues into formalin, process normally and then cut them using RNAse away cleaned microtomes/forceps on a bath of DEPC treated water (in a cleaned water bath). How do other labs prepare paraffin embedded samples? Do you use special reagents - formalin? Do you clean the processor and use treated reagents there too? Do you wash the slides in DEPC treated water? I am trying to figure out how far we need to go here. We are a high throughput lab and we deal with all kinds of tissues and I don't want to have to take down a processor and keep it RNAse free if I don't have to! I will run the experiments and test different methods, but I was hoping some of you could share your wisdom and give me a place to start. Thanks, JJ Jennifer Johnson Staff Scientist Genzyme Corp. Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 Phn - 508-271-3610 Fax - 508-872-9080 From Rhonda.Gregoire <@t> gov.mb.ca Mon Jun 23 13:41:52 2014 From: Rhonda.Gregoire <@t> gov.mb.ca (Gregoire, Rhonda (MAFRD)) Date: Mon Jun 23 13:42:25 2014 Subject: [Histonet] RE: Eosin Leaching In-Reply-To: <201406231702.s5NH2NOB016849@wpgmlir3.imr.gov.mb.ca> References: <201406231702.s5NH2NOB016849@wpgmlir3.imr.gov.mb.ca> Message-ID: Hi Adrienne, We see eosin leaching in our summer months when it is very humid in our lab. The humidity is >70% for most of the summer. We change out all of our alcohols daily to try and prevent it but we sometimes see it even with doing that. Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 ? phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: June-23-14 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Eosin Leaching (Tony Reilly) 2. Histology Benchmarking Survey (Lesley Bechtold) ---------------------------------------------------------------------- Message: 1 Date: Mon, 23 Jun 2014 09:22:28 +1000 From: Tony Reilly Subject: RE: [Histonet] Eosin Leaching To: "Rathborne, Toni" , "Sanders, Jeanine (CDC/OID/NCEZID)" , "'Adrienne Anderson'" , "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Adrienne Also make sure the wash after your bluing agent is higher than the blueing solution or alkalinity of the solution will leach out your eosin. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _________________________________________________ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histonet@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** ------------------------------ Message: 2 Date: Mon, 23 Jun 2014 15:25:27 +0000 From: Lesley Bechtold Subject: [Histonet] Histology Benchmarking Survey To: "histonet@lists.utsouthwestern.edu" Message-ID: <08997064E2075247A4DD5A035DB51CDF64FA0094@jaxbhexms02.jax.org> Content-Type: text/plain; charset="us-ascii" Dear Histonetters, We are seeking information from our peers in the Histology world. To better understand what other Histology Labs offer, we have created a short survey that we hope you will fill out. The link to the survey is below. Information is a useful tool when preparing budgets, hiring staff, buying equipment or making the decision to add new processes to your lab. Anyone who fills out the survey will receive a copy of the de-identified results. We thank you in advance for participating. If surveys annoy you, please delete this email. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) https://www.surveymonkey.com/s/HistoSurvey2014 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 28 ***************************************** From DSiena <@t> statlab.com Mon Jun 23 13:47:52 2014 From: DSiena <@t> statlab.com (Debra Siena) Date: Mon Jun 23 13:47:58 2014 Subject: [Histonet] Eosin leaching Message-ID: Hi Histonet, Eosin bleaching is usually due to the alcohols that are used after the eosin, if you are in a humid area, the alcohols can actually absorb water from the humidity in the air. This may mean a change of how often you rotate or change the alcohols, possibly more often than you did during the winter months when usually less humid. Also if you use a graded alcohol after the eosin, maybe worth looking into going straight to 100%, I usually always recommend at least 3 changes 100% alcohol for 1 minute and 3 changes of xylene for 1 minute. If the last alcohol in your staining line is pink, it means that there is eosin and that means that there is water. Eosin loves water and loves to come out of solution into the one containing the water. I have even seen pink xylene in some staining lines due to water in xylene, however, not enough that it has turned cloudy yet but still have a bit of water. Xylene will tolerate about .5% water before becoming too milky or cloudy to use. Also when you rotate your solutions make sure that you dry the containers well before refilling and by graduating the levels of the alcohols and xylenes to make sure that your last container completely covers the slides. Lastly, adding a 95% alcohol before your eosin, if you don't have one may help to keep excess water out of your eosin which will cause the eosin to break down faster, again eosin loves water. Just spouting off the top of my head but hope something helps out. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com From dscoope <@t> emory.edu Mon Jun 23 14:12:42 2014 From: dscoope <@t> emory.edu (Cooper, Deborah S) Date: Mon Jun 23 14:12:50 2014 Subject: [Histonet] Part time/on call position available - Atlanta, GA Message-ID: <0A373A594DC74F4481701FE3D554F79FA00A28DC@e14mbx12n.Enterprise.emory.net> Hi, We are looking for a person who can come in part-time/on call to cut paraffin sections only. The person does not need to be a certified histotech, just someone who either has cutting experience or is willing to learn and has excellent attention to detail. We would need the person to come in a day or two after being called (on a sporadic basis) and would average about 4 to 8 hours a week. Position is well suited for a retiree. Thank you, Deborah Deborah Cooper Research Specialist Center for Neurodegenerative Disease Emory University 615 Michael St WRB Rm 515 Atlanta, GA 30322 404-712-8353 ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From KSimeone <@t> leavittmgt.com Mon Jun 23 14:24:48 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon Jun 23 14:24:54 2014 Subject: [Histonet] IHC proficiency testing Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C2FD9F0C5@vm-email.leavittmgt.com> Can anyone share their CLIA proficiency protocols/programs for automated IHC staining? Other than the obvious antibody validation, positive and negative controls, QA and QC performed, should we enroll in an actual program for our IHC? We currently use two Leica Bonds and about 70 antibodies. No Her2 or ER/PR or FISH. Thanks in advance! K. Simeone ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From lcolbert <@t> pathmdlabs.com Mon Jun 23 14:37:13 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Mon Jun 23 14:37:19 2014 Subject: [Histonet] Slides for Thermo Slidemate Message-ID: <12ECD7346266D74691EC2BFC75285E452F49AD4F@BFL323E10.pathmdlabs.local> What slides are others using (other than Thermo's Superfrost Plus slides) that won't jam in the Slidemate and that will produce print that does not smear? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From deganh <@t> upstate.edu Mon Jun 23 15:10:48 2014 From: deganh <@t> upstate.edu (Helene Degan) Date: Mon Jun 23 15:11:00 2014 Subject: [Histonet] SV 40 Message-ID: <53A851880200007A0002E892@gatedom1.upstate.edu> Hi Wondering where other laboratories performing the SV40 anitbody for IHC are obtaining control tissue from, is it purchased from a company if so which one? We are currently performing the SV 40 IHC on Ventana Ultra/ XT and we are not getting the appropriate staining, any help would be greatly appreciated. Thanks Helene Assistant Supervisor of Histology Upstate Medical University From wbenton <@t> cua.md Mon Jun 23 16:27:20 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Mon Jun 23 16:28:11 2014 Subject: [Histonet] RE: Slides for Thermo Slidemate In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F49AD4F@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F49AD4F@BFL323E10.pathmdlabs.local> Message-ID: <0B8979A204680A42B93A52B486088CD93938636891@CUAEXH1.GCU-MD.local> Lecia makes a Thermal charged slide that we just tried and it works great. Best print I've seen to date and no smearing. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert [lcolbert@pathmdlabs.com] Sent: Monday, June 23, 2014 3:37 PM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Slides for Thermo Slidemate What slides are others using (other than Thermo's Superfrost Plus slides) that won't jam in the Slidemate and that will produce print that does not smear? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From LSebree <@t> uwhealth.org Mon Jun 23 18:18:26 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Jun 23 18:18:31 2014 Subject: [Histonet] RE: IHC proficiency testing In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C2FD9F0C5@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C2FD9F0C5@vm-email.leavittmgt.com> Message-ID: <77DD817201982748BC67D7960F2F76AF0BA921@UWHC-MBX12.uwhis.hosp.wisc.edu> Hi Kari, I'd suggest taking advantage of CAP proficiency testing surveys so as to compare your lab's results to others. Linda A. Sebree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Delray Beach Pathology Kari Simeone [KSimeone@leavittmgt.com] Sent: Monday, June 23, 2014 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC proficiency testing Can anyone share their CLIA proficiency protocols/programs for automated IHC staining? Other than the obvious antibody validation, positive and negative controls, QA and QC performed, should we enroll in an actual program for our IHC? We currently use two Leica Bonds and about 70 antibodies. No Her2 or ER/PR or FISH. Thanks in advance! K. Simeone ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Mon Jun 23 23:14:51 2014 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Mon Jun 23 23:15:04 2014 Subject: [Histonet] need recommendations on working alpha synulcein IHC for human tissue Message-ID: <96ED45A1-A42D-44EB-80D8-1C29BC4FD970@gmail.com> I'm looking for a working alpha synuclein IHC antibody to be used on human tissue. I work on human brain stem free-floating 60um sections. I would greatly appreciate any suggestions & recommendations from anyone. Regards Maria Mejia Histology Supervisor UCSF Department of Neurology SF, CA From brett_connolly <@t> merck.com Tue Jun 24 07:52:59 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Jun 24 07:53:14 2014 Subject: [Histonet] need recommendations on working alpha synulcein IHC for human tissue In-Reply-To: <96ED45A1-A42D-44EB-80D8-1C29BC4FD970@gmail.com> References: <96ED45A1-A42D-44EB-80D8-1C29BC4FD970@gmail.com> Message-ID: I would also be interested in what Alpha synuclein Abs people are using for IHC Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maria Mejia Sent: Tuesday, June 24, 2014 12:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need recommendations on working alpha synulcein IHC for human tissue I'm looking for a working alpha synuclein IHC antibody to be used on human tissue. I work on human brain stem free-floating 60um sections. I would greatly appreciate any suggestions & recommendations from anyone. Regards Maria Mejia Histology Supervisor UCSF Department of Neurology SF, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From cmconway <@t> usgs.gov Tue Jun 24 09:14:06 2014 From: cmconway <@t> usgs.gov (Conway, Carla) Date: Tue Jun 24 09:14:12 2014 Subject: [Histonet] Can acetic acid in fixative affect fungal staining by PAS? Message-ID: Hello, I fixed skin/muscle and gill tissues for 48 h in Dietrich's fixative (30 mL 95 % ethanol, 10 mL formalin, 2 mL glacial acetic acid and 58 mL deionized water) and used the Hotchkiss-McManus PAS protocol to stain *Saprolegnia *fungi. The fungi are light green from the counterstain and not PAS-positive. I used fresh staining reagents and am wondering if the acetic acid in the Dietrich's fixative may have affected the cell walls. I'd appreciate your comments and suggestions. Thanks very much! Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov From Luis.Chiriboga <@t> nyumc.org Tue Jun 24 10:12:01 2014 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Tue Jun 24 10:12:14 2014 Subject: [Histonet] BrDU Message-ID: <3E6798F00C9F494399E96B720ECD14292B2982@MSGWCDCPMB22.nyumc.org> Hi Histonetters Can anyone recommend a good anti-BrDU antibody and protocol Thanks & Happy Summer!! ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From Michael.LaFriniere <@t> ccplab.com Tue Jun 24 10:13:12 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Tue Jun 24 10:13:29 2014 Subject: [Histonet] Eosin Leaching In-Reply-To: References: Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29689FBD@AHCMSASEXCH02.my.ahc.local> I have seen this happen with high levels of Isopropanol in alcohols after the eosins, I switch to regular reagent alcohols and seemed to eliminate the bleeding of the eosin. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> nyumc.org Tue Jun 24 10:55:06 2014 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Tue Jun 24 10:55:42 2014 Subject: [Histonet] RE: BrDU In-Reply-To: <3E6798F00C9F494399E96B720ECD14292B2982@MSGWCDCPMB22.nyumc.org> References: <3E6798F00C9F494399E96B720ECD14292B2982@MSGWCDCPMB22.nyumc.org> Message-ID: <3E6798F00C9F494399E96B720ECD14292B2A54@MSGWCDCPMB22.nyumc.org> Apologies, I meant to add for FFPE muse/rat tissue TX's -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Tuesday, June 24, 2014 11:12 AM To: (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] BrDU Hi Histonetters Can anyone recommend a good anti-BrDU antibody and protocol Thanks & Happy Summer!! ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From foreightl <@t> gmail.com Tue Jun 24 11:14:55 2014 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Jun 24 11:15:02 2014 Subject: [Histonet] Slides for Thermo Slidemate In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F49AD4F@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F49AD4F@BFL323E10.pathmdlabs.local> Message-ID: Cancer Diagnostics has an Autofrost clipped corner that completely eliminated our slide jamming issues and doesn't smear. We have 7 of these printers and have had no issue. They have multiple colors,and also have a good silanized Autofrost IHC slides (we get the 90 degree corner for our machines) that does has behaved very well in our Slidemates.. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Mon, Jun 23, 2014 at 3:37 PM, Laurie Colbert wrote: > What slides are others using (other than Thermo's Superfrost Plus slides) > that won't jam in the Slidemate and that will produce print that does not > smear? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and > confidential information, including patient information protected by > federal and state privacy laws. It is intended only for the use of the > person(s) named above. If you are not the intended recipient, you are > hereby notified that any review, dissemination, distribution, or > duplication of this communication is strictly prohibited. If you are not > the intended recipient, please contact the sender by reply email and > destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TJohnson <@t> gnf.org Tue Jun 24 11:26:53 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Jun 24 11:27:04 2014 Subject: [Histonet] Re: Slides for thermo Slidemate Message-ID: <9F3CFEE76E51B64991C7485270890B40498DC2AB@EX5.lj.gnf.org> Those of you having trouble with slides in the Slidemate, please respond to me privately and let me know which ones are causing trouble for you. Thanks! Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From BDeBrosse-Serra <@t> isisph.com Tue Jun 24 12:32:47 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Jun 24 12:33:12 2014 Subject: [Histonet] RE: BrDU In-Reply-To: <3E6798F00C9F494399E96B720ECD14292B2A54@MSGWCDCPMB22.nyumc.org> References: <3E6798F00C9F494399E96B720ECD14292B2982@MSGWCDCPMB22.nyumc.org> <3E6798F00C9F494399E96B720ECD14292B2A54@MSGWCDCPMB22.nyumc.org> Message-ID: Hi Luis, We are using the BrDU In-Situ Detection Kit #551321 from BD Biosciences with good luck. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Tuesday, June 24, 2014 8:55 AM To: (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: BrDU Apologies, I meant to add for FFPE muse/rat tissue TX's -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Tuesday, June 24, 2014 11:12 AM To: (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] BrDU Hi Histonetters Can anyone recommend a good anti-BrDU antibody and protocol Thanks & Happy Summer!! ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sandy.Cope-Yokoyama <@t> childrens.com Tue Jun 24 14:05:11 2014 From: Sandy.Cope-Yokoyama <@t> childrens.com (Sandy Cope-yokoyama) Date: Tue Jun 24 14:05:15 2014 Subject: [Histonet] Posting for a HIstonet member- Position available Message-ID: <6F81318C8F5F984D972778B918A7D769204BE9BB@CMCPBEXMAIL12.Childrens.med> If you're looking for a friendly environment, a new state-of-the-art facility and an employer of choice, SPL is the place for you!! We currently have several openings: * Histotechnicians/Histotechnologists Histo candidates should be an HTL or HT (ASCP) or equivalent. Primary responsibilities include on-site frozen sections including mobile laboratory units. * Lab Aide * Vehicle Service Coordinator All candidates must have a valid drivers license and proof of insurability. SPL is CAP accredited, offers competitive pay, a comprehensive benefits package. SPL pays 100% of employee premiums for Medical, Dental, LTD and Life. We also offer Retirement Plan & Supplemental Insurance. Please forward resumes to tammy@surgicalpathlabs.com 8455 66th Street N Pinellas Park, Fl 33781 Fax - 727-545-1644 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From Jonathan.Arzt <@t> ARS.USDA.GOV Tue Jun 24 09:34:29 2014 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Tue Jun 24 16:04:29 2014 Subject: [Histonet] Histotechnologist Opening Immediately - Plum Island, USDA Message-ID: <7213AC15544DE945843D821E1769E38A067F2F29@001FSN2MPN1-053.001f.mgd2.msft.net> A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old Saybrook, CT. Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400 Salary $44,175.00 to $84,990.00 / Per year commensurate with experience. Federal benefits package. This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. From amosbrooks <@t> gmail.com Tue Jun 24 16:51:35 2014 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Jun 24 16:51:39 2014 Subject: [Histonet] BrDU Message-ID: Hi, I use Sigma's B2531 with 1 hr pretreatment in 1N HCl at 40 deg (in a coplin jar in my waterbath) then 15 min Trypsin (don't forget the CaCl2) at 40 deg. I detect it witb Envision+ but any mouse IgG would work fine. Amos Message: 15 Date: Tue, 24 Jun 2014 15:12:01 +0000 From: "Chiriboga, Luis" Subject: [Histonet] BrDU To: " (Histonet@lists.utsouthwestern.edu)" Message-ID: <3E6798F00C9F494399E96B720ECD14292B2982@MSGWCDCPMB22.nyumc.org> Content-Type: text/plain; charset="us-ascii" Hi Histonetters Can anyone recommend a good anti-BrDU antibody and protocol Thanks & Happy Summer!! From Jonathan.Arzt <@t> ARS.USDA.GOV Tue Jun 24 18:47:41 2014 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Tue Jun 24 18:47:51 2014 Subject: [Histonet] Histotechnologist Job Opening Immediately - Plum Island, USDA In-Reply-To: <7213AC15544DE945843D821E1769E38A067F2F29@001FSN2MPN1-053.001f.mgd2.msft.net> References: <7213AC15544DE945843D821E1769E38A067F2F29@001FSN2MPN1-053.001f.mgd2.msft.net> Message-ID: <7213AC15544DE945843D821E1769E38A067F5178@001FSN2MPN1-053.001f.mgd2.msft.net> A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old Saybrook, CT. Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400 Salary $44,175.00 to $84,990.00 / Per year commensurate with experience. Federal benefits package. This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. From talulahgosh <@t> gmail.com Wed Jun 25 07:38:29 2014 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Wed Jun 25 07:38:37 2014 Subject: [Histonet] ISH preparation In-Reply-To: <0C0766AB928A0E4DB4760C0DB41F91B984A254CC@XSPW10A507T.pharma.aventis.com> References: <0C0766AB928A0E4DB4760C0DB41F91B984A254CC@XSPW10A507T.pharma.aventis.com> Message-ID: That may be what you have to do, as the first day of ISH needs to be RNase free! When I do them, it is 10 slides at a time and we have dishes and a lab bench set aside for RNase free procedures only. Obviously this is not feasible in all workplaces! I would just think about it logically. Use gloves when you can, always. Can you keep the hybridizing part RNase free? I don't know what machines you use for this--we do process the slides manually in glass dishes and then hybridize in a slide container and oven kept for RNase free purposes. After hybridization, you don't really need to be too careful. Now I'm wondering if there's some sort of magical machine that does ISH! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Jun 23, 2014 at 2:06 PM, wrote: > Hi Folks! > I was hoping to get a little input about preparing samples for ISH. > We currently RNAse-away our tools at the time of collection, toss the > tissues into formalin, process normally and then cut them using RNAse away > cleaned microtomes/forceps on a bath of DEPC treated water (in a cleaned > water bath). > > How do other labs prepare paraffin embedded samples? > > Do you use special reagents - formalin? Do you clean the processor and > use treated reagents there too? Do you wash the slides in DEPC treated > water? > > I am trying to figure out how far we need to go here. We are a high > throughput lab and we deal with all kinds of tissues and I don't want to > have to take down a processor and keep it RNAse free if I don't have to! > I will run the experiments and test different methods, but I was hoping > some of you could share your wisdom and give me a place to start. > Thanks, > JJ > > Jennifer Johnson > Staff Scientist > Genzyme Corp. > Department of Pathology > 5 Mountain Road > Framingham, MA 01701-9322 > Phn - 508-271-3610 > Fax - 508-872-9080 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From galinadeyneko <@t> yahoo.com Wed Jun 25 09:32:17 2014 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Wed Jun 25 09:35:21 2014 Subject: [Histonet] BRDu IHC protocol Message-ID: <1403706737.61662.YahooMailNeo@web160205.mail.bf1.yahoo.com> ?Hi Luis I attach my protocol for BRDu Best regards BRDU protocol 1.??????????? De-wax and hydrate through the changes of Xylenes and graded Ethanols (100%, 95%, 70%) the paraffin slides till DI water. 2.??????????? HIER :Performed in Biocare?s Decloaking Chamber with manufacture settings ( set point 1-125?C, 30 seconds, set point 2- 90?C , 10 seconds) in plastic container with 1X Rodent Decloaker solution which is stable modified Citrate Buffer pH 6.00, (Biocare # RD913). Optional: ???? Place the slides in preheated till 90- 100 ?C in 1X Rodent Decloaker solution which is stable modified Citrate Buffer pH 6.00, (Biocare # RD913) and steam 45 minutes in steamer .Remove the slides from steamer and allow them to cool till RT? 3.??????????? Rinse in DI water and Wash in Tris buffer three times x 5 minutes 4.??????????? Circle the tissue on the slides with hydrophobic barrier using PAP pen 5.??????????? Incubate with pepsin enzyme digestion solution ? 2 minutes. 6.??????????? Prepare fresh before using: 0.25%? solution of pepsin (250 mg of powder in 100 ml of Tris) (powder, Biomedicals, # 195367 ) in? 1X Tris buffer( 10X Fisher Scientific, BP2471), titrated to pH 2.00 with concentrated hydrochloric acid. Optional: Preparation of 0.05M Tris(which correspond to 1X Tris) from 1M Tris (Teknova. ,cat.# T1068,pH6.8)- 10ml of 1 M Tris + 190 ml of DI water. (53 mg of powdered pepsin in 22.200 ml of Tris, pH2.5) 7.??????????? Neutralize the slides 2 times x 5 minutes each in the 0,1 M Borate Buffer , pH 8.5 (0.5M sodium Borate Buffer, Boston Bioproducts, # BB-66, 1 part of 0.5M buffer + 4 parts of DI water). 8.??????????? Wash in Tris buffer three times x 5 minutes. 9.??????????? Quench the endogenous peroxidase with PEROXIDAZED 1 (Biocare # PX 968)? for 15 minutes at RT 10.????????? Rinse in DI water and Wash in Tris buffer two times x 5 minutes 11.????????? Block endogenous Protein? with Background Sniper for 30 minutes at RT ( Biocare , #BS966) 12.????????? Tap off the excess of protein block , do not rinse 13.????????? Incubate the slides with the primary antibody (mouse monoclonal anti- bromodeoxyuridine, Roche, # 11 170 376001) 80 minutes. Diluent: ready-to-use antibody diluent, (Dako, # S0809). Dilutions:? 1:700 Wash in Tris buffer three? times x 5 minutes 14.????????? Incubate the slides with mouse ?on-mouse HRP Polymer (Biocare, #MM620) for 30 minutes at RT.-for mouse tissues or with mouse on rat HRP polymer (Biocare, # MRT 621) for rat tissues. 15.????????? Wash in Tris buffer two times x 5 minutes 16.????????? Stain the slides with freshly prepared DAB chromogen (Dako, #K3468) and developed end-colored product in the targeted cells under microscope. Staining is complete when the protein positive areas turn to dark brown and protein negative areas (background) remain colorless. Stop the chromogen reaction by placing the slides in DI water with following rinse in fresh DI water to remove the residual DAB and clear the slides.- 5 minutes. Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w From mw <@t> personifysearch.com Wed Jun 25 13:54:47 2014 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Jun 25 13:54:58 2014 Subject: [Histonet] New Field Based Histology Opportunities Message-ID: <088501cf90a6$f0b3a670$d21af350$@personifysearch.com> Good Afternoon! We have had a number of new field based histology openings throughout the US. We are targeting Histology professionals who are looking for an exciting career outside of the laboratory and in the field. The company is a world leader in histology and offers a strong career track. We currently have openings in: NYC/NJ - IHC NC/SC/GA - Core Histology Midwest - Based near a major airport - Core Histology Northern California - Core Histology If you or someone you may know is interested, please send us an e-mail with your updated resume and availability to catch up and talk in more detail. Please send e-mails to mw@personifysearch.com . Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com From ibernard <@t> uab.edu Wed Jun 25 14:33:53 2014 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Wed Jun 25 14:34:06 2014 Subject: [Histonet] Template Special Stain validation Form or Sheet Message-ID: Any suggestions or examples (from CAP, ASCP or NSH) on a template validation sheet that we could use for documentation and for CAP inspection purposes? Is there a recommended test or sample size. We have positive controls for all our test menu stains. Need a reference for all above. Ian Bernard From algranth <@t> email.arizona.edu Wed Jun 25 14:55:31 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Jun 25 14:55:41 2014 Subject: [Histonet] looking for tips on cutting frozen fly heads Message-ID: <767401CC-F5E6-43EF-8CDD-F21889D47A5B@email.arizona.edu> I have another of those unusual projects and I'm looking for some advice of handling the drosophila flies before freezing (to fix or not to fix) and then freezing tips. Anybody have any experience with these flies? They brought me some this morning but the results were not good. They killed the flies in 95% ETOH and strung them on a "fly collar" and put them in OCT and froze with a dry ice-alcohol slurry. Looks like ice crystals and a lot of shrinking and shattering under the scope. Andi G Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From akemiat3377 <@t> gmail.com Wed Jun 25 21:07:50 2014 From: akemiat3377 <@t> gmail.com (Eileen Akemi Allison) Date: Wed Jun 25 21:07:55 2014 Subject: [Histonet] Project DNA-Hereditary Cancers Message-ID: <793129D2-5A32-483C-8468-A93D5C83A98B@gmail.com> FYI to my Histonet peeps. New developments in Hereditary Cancer. I am so proud to be associated with such a progressive GI doctor who has a passion towards genetic cancer solutions! Check out what the CEO of our GI practice/pathology laboratory, Dr. Daniel Luba's passion is on this website. He is the Chairman of the Board for project DNA. http://theprojectdna.com/about/ With warmest regards, Akemi Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aallison@montereygi.com Tele: (831) 375-3577 X117 From jmoreira <@t> sidra.org Wed Jun 25 23:33:14 2014 From: jmoreira <@t> sidra.org (Joana Moreira) Date: Wed Jun 25 23:33:24 2014 Subject: [Histonet] RE: ISH preparation Message-ID: Leica's Thermobrite Elite? http://www.leicabiosystems.com/pathology-imaging/cytogenetics/details/product/leica-thermobrite-elite/ Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Message: 6 Date: Wed, 25 Jun 2014 08:38:29 -0400 From: Emily Brown Subject: Re: [Histonet] ISH preparation To: Jennifer.Johnson@genzyme.com Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 That may be what you have to do, as the first day of ISH needs to be RNase free! When I do them, it is 10 slides at a time and we have dishes and a lab bench set aside for RNase free procedures only. Obviously this is not feasible in all workplaces! I would just think about it logically. Use gloves when you can, always. Can you keep the hybridizing part RNase free? I don't know what machines you use for this--we do process the slides manually in glass dishes and then hybridize in a slide container and oven kept for RNase free purposes. After hybridization, you don't really need to be too careful. Now I'm wondering if there's some sort of magical machine that does ISH! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Jun 23, 2014 at 2:06 PM, wrote: > Hi Folks! > I was hoping to get a little input about preparing samples for ISH. > We currently RNAse-away our tools at the time of collection, toss the > tissues into formalin, process normally and then cut them using RNAse > away cleaned microtomes/forceps on a bath of DEPC treated water (in a > cleaned water bath). > > How do other labs prepare paraffin embedded samples? > > Do you use special reagents - formalin? Do you clean the processor > and use treated reagents there too? Do you wash the slides in DEPC > treated water? > > I am trying to figure out how far we need to go here. We are a high > throughput lab and we deal with all kinds of tissues and I don't want > to have to take down a processor and keep it RNAse free if I don't have to! > I will run the experiments and test different methods, but I was > hoping some of you could share your wisdom and give me a place to start. > Thanks, > JJ > > Jennifer Johnson > Staff Scientist > Genzyme Corp. > Department of Pathology > 5 Mountain Road > Framingham, MA 01701-9322 > Phn - 508-271-3610 > Fax - 508-872-9080 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From jqb7 <@t> cdc.gov Thu Jun 26 05:45:30 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu Jun 26 05:46:00 2014 Subject: [Histonet] Recycled or not? Message-ID: <3B2CD438E1628A41BD687E98B963B7811FDB94DD@EMBX-CHAM2.cdc.gov> Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov From sforeman <@t> labpath.com Thu Jun 26 07:18:08 2014 From: sforeman <@t> labpath.com (Susan Foreman) Date: Thu Jun 26 07:23:12 2014 Subject: [Histonet] Ventana iScan Coreo Slide Scanner Message-ID: <001701cf9138$b0ce4d20$126ae760$@com> Does anyone in the United States provide service for the Ventana Coreo Slide Scanner other than Ventana? Many Thanks, Susan From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Jun 26 08:05:36 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Jun 26 08:05:47 2014 Subject: [Histonet] RE: Recycled or not? In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FDB94DD@EMBX-CHAM2.cdc.gov> References: <3B2CD438E1628A41BD687E98B963B7811FDB94DD@EMBX-CHAM2.cdc.gov> Message-ID: <1403787932089.4136@accuratediagnosticlabs.com> While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Thu Jun 26 09:33:45 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Jun 26 09:33:58 2014 Subject: [Histonet] RE: Recycled or not? NO PHI In-Reply-To: <1403787932089.4136@accuratediagnosticlabs.com> References: <3B2CD438E1628A41BD687E98B963B7811FDB94DD@EMBX-CHAM2.cdc.gov> <1403787932089.4136@accuratediagnosticlabs.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632615317B5C@LRGHEXVS1.practice.lrgh.org> We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Jun 26 09:36:41 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Jun 26 09:36:52 2014 Subject: [Histonet] RE: Recycled or not? NO PHI In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632615317B5C@LRGHEXVS1.practice.lrgh.org> References: <3B2CD438E1628A41BD687E98B963B7811FDB94DD@EMBX-CHAM2.cdc.gov> <1403787932089.4136@accuratediagnosticlabs.com>, <38667E7FB77ECD4E91BFAEB8D98638632615317B5C@LRGHEXVS1.practice.lrgh.org> Message-ID: <1403793396187.61113@accuratediagnosticlabs.com> Maybe your pathologists aren't as fussy as the pathologists I worked with at the time. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: Podawiltz, Thomas Sent: Thursday, June 26, 2014 1:33 PM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From lblazek <@t> digestivespecialists.com Thu Jun 26 09:43:25 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jun 26 09:43:35 2014 Subject: [Histonet] RE: Recycled or not? NO PHI In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632615317B5C@LRGHEXVS1.practice.lrgh.org> References: <3B2CD438E1628A41BD687E98B963B7811FDB94DD@EMBX-CHAM2.cdc.gov> <1403787932089.4136@accuratediagnosticlabs.com> <38667E7FB77ECD4E91BFAEB8D98638632615317B5C@LRGHEXVS1.practice.lrgh.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E39168FD14CE9@IBMB7Exchange.digestivespecialists.com> I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Thu Jun 26 10:22:35 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Jun 26 10:22:46 2014 Subject: [Histonet] RE: Recycled or not? NO PHI In-Reply-To: <1403793396187.61113@accuratediagnosticlabs.com> References: <3B2CD438E1628A41BD687E98B963B7811FDB94DD@EMBX-CHAM2.cdc.gov> <1403787932089.4136@accuratediagnosticlabs.com>, <38667E7FB77ECD4E91BFAEB8D98638632615317B5C@LRGHEXVS1.practice.lrgh.org> <1403793396187.61113@accuratediagnosticlabs.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632615317B6C@LRGHEXVS1.practice.lrgh.org> They are very fussy as you put it. However, I am even more anal than they are about our work. We do QA checks both before and after we recycle. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: Barbara Tibbs [mailto:barbara.tibbs@accuratediagnosticlabs.com] Sent: Thursday, June 26, 2014 10:37 AM To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI Maybe your pathologists aren't as fussy as the pathologists I worked with at the time. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: Podawiltz, Thomas Sent: Thursday, June 26, 2014 1:33 PM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Sherrian.McAnn <@t> va.gov Thu Jun 26 10:20:24 2014 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Thu Jun 26 10:24:11 2014 Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI Message-ID: <61E2B58CECEF384094A363989D47C090479D1B@VHAV17MSGA2.v17.med.va.gov> We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Jun 26 10:33:04 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jun 26 10:33:23 2014 Subject: [Histonet] RE: Recycled or not? NO PHI In-Reply-To: <61E2B58CECEF384094A363989D47C090479D1B@VHAV17MSGA2.v17.med.va.gov> References: <61E2B58CECEF384094A363989D47C090479D1B@VHAV17MSGA2.v17.med.va.gov> Message-ID: I have used recycled xylene since the mid-80s and the only problem is that it is purer than new xylene and can make biopsies crispy. (The isomers get distilled out.) We use new xylene on the biopsy processor. The recycler is in our lab and there are no fumes at all. Surely does save money. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Thursday, June 26, 2014 11:20 AM To: Blazek, Linda; Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Jun 26 10:48:48 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Jun 26 10:49:24 2014 Subject: [Histonet] RE: Recycled or not? NO PHI In-Reply-To: References: <61E2B58CECEF384094A363989D47C090479D1B@VHAV17MSGA2.v17.med.va.gov>, Message-ID: <1403797723648.35302@accuratediagnosticlabs.com> Hmm. Maybe the company who manufactured our recycler went out of business or we got a lemon. As soon as we abandoned recycling and went back to new reagents, the stains and processing were perfect. Go figure. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce K. Sent: Thursday, June 26, 2014 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI I have used recycled xylene since the mid-80s and the only problem is that it is purer than new xylene and can make biopsies crispy. (The isomers get distilled out.) We use new xylene on the biopsy processor. The recycler is in our lab and there are no fumes at all. Surely does save money. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Thursday, June 26, 2014 11:20 AM To: Blazek, Linda; Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Thu Jun 26 10:55:25 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Jun 26 10:55:44 2014 Subject: [Histonet] RE: Recycled or not? NO PHI In-Reply-To: References: <61E2B58CECEF384094A363989D47C090479D1B@VHAV17MSGA2.v17.med.va.gov> Message-ID: <1400452838.9645459.1403798125323.JavaMail.root@comcast.net> Hi Joyce, Absolutely agree with recycling concept, value, money saved and no fumes in lab (if using newer models) and if used properly.? I've always been curious about the concept of a lab recycler making xylene "purer" by distilling out isomers.? Which unit do you have?? meta-xylene is in great demand as a feedstock for plastic production.? Since xylene(s) are a mixture of ortho-, meta- and para all of which differ in boiling points by just very few degrees, they are (near) impossible to separate out from one another by ordinary distillation and need multi-fractional set-ups?with crystallization and absorption and catalytic beds.? Manufacturers spend vast sums to do this and are always looking for a better way.? What unit do you have?? Have you had chromatography done on your (new) input and then output xylene.? I've done it extensively for alcohol but never xylene. Thanks, ? Ray Seattle, WA ----- Original Message ----- From: "Joyce K. Weems" To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 26, 2014 8:33:04 AM Subject: [Histonet] RE: Recycled or not? ?NO PHI I have used recycled xylene since the mid-80s and the only problem is that it is purer than new xylene and can make biopsies crispy. (The isomers get distilled out.) We use new xylene on the biopsy processor. The recycler is in our lab and there are no fumes at all. Surely does save money. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). ?It may contain information that is privileged and confidential. ?Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Thursday, June 26, 2014 11:20 AM To: Blazek, Linda; Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. ?P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. ?Those were not the good ole days. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. ?With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. ?We check each batch as it is recycled. We also don't have a problem with fumes. ?(And our pathologists are fussy) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. ?There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. ?If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ahorvath <@t> cogipath.com Thu Jun 26 11:04:00 2014 From: ahorvath <@t> cogipath.com (Andrew Horvath) Date: Thu Jun 26 11:03:50 2014 Subject: [Histonet] RE: Recycled or not? NO PHI In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632615317B6C@LRGHEXVS1.practice.lrgh.org> References: <3B2CD438E1628A41BD687E98B963B7811FDB94DD@EMBX-CHAM2.cdc.gov> <1403787932089.4136@accuratediagnosticlabs.com>, <38667E7FB77ECD4E91BFAEB8D98638632615317B5C@LRGHEXVS1.practice.lrgh.org> <1403793396187.61113@accuratediagnosticlabs.com> <38667E7FB77ECD4E91BFAEB8D98638632615317B6C@LRGHEXVS1.practice.lrgh.org> Message-ID: <004101cf9158$3e202f80$ba608e80$@cogipath.com> We only recycle xylene which we've tested purity and was close to 98-99% and is used in both H&E staining and processing with no issue. I've done 8 hour xylene vapor monitoring in our recycling room, while recycling was occurring, and the results were 6.0ppm where the OSHA exposure limit is 100.0ppm. Andrew Horvath, MBA, MA Operations Manager 303-770-4848 (O) 303-770-6641 (fax) ? Colorado GI Pathology 7346 S. Alton Way Suite 10E Centennial, CO 80112 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 9:23 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI They are very fussy as you put it. However, I am even more anal than they are about our work. We do QA checks both before and after we recycle. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: Barbara Tibbs [mailto:barbara.tibbs@accuratediagnosticlabs.com] Sent: Thursday, June 26, 2014 10:37 AM To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI Maybe your pathologists aren't as fussy as the pathologists I worked with at the time. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: Podawiltz, Thomas Sent: Thursday, June 26, 2014 1:33 PM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Arzt <@t> ARS.USDA.GOV Thu Jun 26 11:20:11 2014 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Thu Jun 26 11:20:50 2014 Subject: [Histonet] Histotechnologist Opening Immediately - Plum Island, USDA Message-ID: <7213AC15544DE945843D821E1769E38A067F9F93@001FSN2MPN1-053.001f.mgd2.msft.net> A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old Saybrook, CT. Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400 Salary $44,175.00 to $84,990.00 / per year commensurate with experience. Federal benefits package. This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. From jstaruk <@t> masshistology.com Thu Jun 26 11:29:01 2014 From: jstaruk <@t> masshistology.com (Mass Histology) Date: Thu Jun 26 11:29:16 2014 Subject: [Histonet] RE: Recycle or not? Message-ID: <1e6901cf915b$c4a93a30$4dfbae90$@masshistology.com> This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com From tbraud <@t> holyredeemer.com Thu Jun 26 11:33:01 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jun 26 11:33:15 2014 Subject: [Histonet] RE: ISH Automation In-Reply-To: <20140626151133.A96391E8060@trendmess-svr.holyredeemer.local> References: <20140626151133.A96391E8060@trendmess-svr.holyredeemer.local> Message-ID: Hi - there are a couple of instruments that automate ISH and RISH. Our lab was a beta site for BioCare's Oncore and we loved it. It has an open platform and is very user friendly. The Oncore is a very compact instrument and the maintenance was easy and minimal. All of the techs here liked it. Check it out. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 6 Date: Wed, 25 Jun 2014 08:38:29 -0400 From: Emily Brown Subject: Re: [Histonet] ISH preparation To: Jennifer.Johnson@genzyme.com That may be what you have to do, as the first day of ISH needs to be RNase free! When I do them, it is 10 slides at a time and we have dishes and a lab bench set aside for RNase free procedures only. Obviously this is not feasible in all workplaces! I would just think about it logically. Use gloves when you can, always. Can you keep the hybridizing part RNase free? I don't know what machines you use for this--we do process the slides manually in glass dishes and then hybridize in a slide container and oven kept for RNase free purposes. After hybridization, you don't really need to be too careful. Now I'm wondering if there's some sort of magical machine that does ISH! Emily ************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Joyce.Weems <@t> emoryhealthcare.org Thu Jun 26 11:46:48 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jun 26 11:47:01 2014 Subject: [Histonet] RE: Recycle or not? In-Reply-To: <1e6901cf915b$c4a93a30$4dfbae90$@masshistology.com> References: <1e6901cf915b$c4a93a30$4dfbae90$@masshistology.com> Message-ID: Haven't heard this one till now! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mass Histology Sent: Thursday, June 26, 2014 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycle or not? This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Valerie.Hannen <@t> parrishmed.com Thu Jun 26 11:51:50 2014 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Thu Jun 26 11:52:07 2014 Subject: [Histonet] RE: Recycle or not? In-Reply-To: References: <1e6901cf915b$c4a93a30$4dfbae90$@masshistology.com> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB7AB@isexstore03> Well..that's a new one!!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, June 26, 2014 12:47 PM To: 'Mass Histology'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Recycle or not? Haven't heard this one till now! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mass Histology Sent: Thursday, June 26, 2014 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycle or not? This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim _______________________ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From Joyce.Weems <@t> emoryhealthcare.org Thu Jun 26 12:25:37 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jun 26 12:25:53 2014 Subject: [Histonet] RE: Recycled or not? NO PHI In-Reply-To: <1403797723648.35302@accuratediagnosticlabs.com> References: <61E2B58CECEF384094A363989D47C090479D1B@VHAV17MSGA2.v17.med.va.gov>, <1403797723648.35302@accuratediagnosticlabs.com> Message-ID: Gotta go with what works!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Barbara Tibbs [mailto:barbara.tibbs@accuratediagnosticlabs.com] Sent: Thursday, June 26, 2014 11:49 AM To: Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI Hmm. Maybe the company who manufactured our recycler went out of business or we got a lemon. As soon as we abandoned recycling and went back to new reagents, the stains and processing were perfect. Go figure. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Weems, Joyce K. Sent: Thursday, June 26, 2014 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI I have used recycled xylene since the mid-80s and the only problem is that it is purer than new xylene and can make biopsies crispy. (The isomers get distilled out.) We use new xylene on the biopsy processor. The recycler is in our lab and there are no fumes at all. Surely does save money. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Thursday, June 26, 2014 11:20 AM To: Blazek, Linda; Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Thu Jun 26 13:18:59 2014 From: JWatson <@t> gnf.org (James Watson) Date: Thu Jun 26 13:19:08 2014 Subject: [Histonet] RE: Recycle or not? In-Reply-To: <1e6901cf915b$c4a93a30$4dfbae90$@masshistology.com> References: <1e6901cf915b$c4a93a30$4dfbae90$@masshistology.com> Message-ID: This topic was discussed at a meeting somewhere that I attended and here with our safety staff. The work around that we do here to conform to waste disposal regulations here in Ca. is that xylene for recycling is not classified as waste, so it does not enter the waste stream. Since you are not recycling waste you are not practicing "waste management". James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mass Histology Sent: Thursday, June 26, 2014 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycle or not? This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Thu Jun 26 14:01:30 2014 From: jstaruk <@t> masshistology.com (Mass Histology) Date: Thu Jun 26 14:01:31 2014 Subject: [Histonet] RE: Recycle or not? In-Reply-To: References: <1e6901cf915b$c4a93a30$4dfbae90$@masshistology.com> Message-ID: <1e8b01cf9171$0b90ba80$22b22f80$@masshistology.com> I used this exact argument in my defense (as suggested by the manufacturer of my recycler) and the EPA responded with the following: "You should consider discussing your recycling activity with MassDEP (the state agency). Every state has unique interpretations on when a recycling license or permit is required--and the manufacturer of a device is not the best contact to make that decision". Jim _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com -----Original Message----- From: James Watson [mailto:JWatson@gnf.org] Sent: Thursday, June 26, 2014 2:19 PM To: 'Mass Histology'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Recycle or not? This topic was discussed at a meeting somewhere that I attended and here with our safety staff. The work around that we do here to conform to waste disposal regulations here in Ca. is that xylene for recycling is not classified as waste, so it does not enter the waste stream. Since you are not recycling waste you are not practicing "waste management". James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mass Histology Sent: Thursday, June 26, 2014 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycle or not? This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com From TJohnson <@t> gnf.org Thu Jun 26 14:23:44 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Jun 26 14:23:58 2014 Subject: [Histonet] Re: Recycle or not? Message-ID: <9F3CFEE76E51B64991C7485270890B40498DE825@EX5.lj.gnf.org> My condolences, Jim. Yes, that happened to me in my former place of employment. It was not fun. To the companies who provide recycling equipment, it would behoove you to be aware of federal guidelines and how it might not only affect your ability to sell new units, but also how it affects current customers who may have to deal with issues from regulatory agencies. A little bit of guidance in these matters would be quite helpful to us. Best wishes, Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From Nancy_Schmitt <@t> pa-ucl.com Thu Jun 26 16:55:05 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Jun 26 16:55:12 2014 Subject: [Histonet] from saline to formalin Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB265844@PEITHA.wad.pa-ucl.com> Do you know where there might be a reference for?.?How long can a specimen be in saline until it should be placed in formalin or other fixative?? Thank you Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Richard.Cartun <@t> hhchealth.org Thu Jun 26 17:06:09 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Jun 26 17:06:15 2014 Subject: [Histonet] RE: from saline to formalin In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB265844@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB265844@PEITHA.wad.pa-ucl.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E301ED41C@HHCEXCHMB05.hhcsystem.org> The answer to that question will depend on what you are going to do with the tissue (H&E, IHC, or molecular testing)? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, June 26, 2014 5:55 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] from saline to formalin Do you know where there might be a reference for...."How long can a specimen be in saline until it should be placed in formalin or other fixative?" Thank you Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From cecystan76 <@t> gmail.com Thu Jun 26 18:02:48 2014 From: cecystan76 <@t> gmail.com (Cecilia Austin) Date: Thu Jun 26 18:02:57 2014 Subject: [Histonet] from saline to formalin In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB265844@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB265844@PEITHA.wad.pa-ucl.com> Message-ID: Hi Nancy, I found this paper (unfortunately I don't have access to the full article) that may be of help; they looked at various time points for holding the tissue in saline before being fixed in formalin. http://www.ncbi.nlm.nih.gov/pubmed/24822006 J Maxillofac Oral Surg. 2014 Jun;13(2):148-51. doi: 10.1007/s12663-013-0473-z. Epub 2013 Jan 26. Artefacts produced by normal saline when used as a holding solution for biopsy tissues in transit. Sengupta S1, Prabhat K2, Gupta V2, Vij H2, Vij R2, Sharma V2. Cheers, Cecilia On Jun 26, 2014, at 4:55 PM, Nancy Schmitt wrote: > Do you know where there might be a reference for?.?How long can a specimen be in saline until it should be placed in formalin or other fixative?? > > Thank you > > Nancy Schmitt MLT, HT(ASCP) > United Clinical Laboratories > Dubuque, IA 52001 > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From njoydobro <@t> aol.com Fri Jun 27 05:07:29 2014 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Fri Jun 27 05:07:35 2014 Subject: [Histonet] New Field Based Histology Opportunities In-Reply-To: <088501cf90a6$f0b3a670$d21af350$@personifysearch.com> References: <088501cf90a6$f0b3a670$d21af350$@personifysearch.com> Message-ID: <8D16006BC6D173C-21E8-1B861@webmail-vd007.sysops.aol.com> I am attaching my resume for your consideration. Thanks in advance, Roxanne -----Original Message----- From: Matt Ward To: Histonet Sent: Wed, Jun 25, 2014 11:56 am Subject: [Histonet] New Field Based Histology Opportunities Good Afternoon! We have had a number of new field based histology openings throughout the US. We are targeting Histology professionals who are looking for an exciting career outside of the laboratory and in the field. The company is a world leader in histology and offers a strong career track. We currently have openings in: NYC/NJ - IHC NC/SC/GA - Core Histology Midwest - Based near a major airport - Core Histology Northern California - Core Histology If you or someone you may know is interested, please send us an e-mail with your updated resume and availability to catch up and talk in more detail. Please send e-mails to mw@personifysearch.com . Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Fri Jun 27 07:11:20 2014 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Jun 27 07:11:25 2014 Subject: [Histonet] Friday histology trivia Message-ID: <8D160180A61F745-533C-1B6A2@webmail-vm019.sysops.aol.com> Any one who has seen the CBS evening news with Scott Pelley lately (at least in my area) will have seen an ad for a product named "Salonpas". In the background is a microscope. It is interesting that when you see a microscope in an ad on TV, it implies that real, dedicated research has been performed in bringing the product to market. In this case, the microscope looks like a 30 year old plus American Optical microscope. I don't think AO are in busincess any more. Maybe Leica took them over. Looks good though! I have never seen a microtome in an ad on TV, but plenty of microscopes! Michael Titford USA Pathology Mobile AL USA From talulahgosh <@t> gmail.com Fri Jun 27 10:57:38 2014 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Fri Jun 27 10:57:42 2014 Subject: [Histonet] Histology as art! Message-ID: Hello Histonetters, I'm really looking forward to going to the brand new Morbid Anatomy Museum in NYC, but imagine my surprise when I found some histology in their online gift shop!! http://morbidanatomy.bigcartel.com/category/gifts Histology is beautiful, but it is odd to look at those images on clothing. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" From barbara.tibbs <@t> accuratediagnosticlabs.com Fri Jun 27 11:14:06 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Fri Jun 27 11:14:12 2014 Subject: [Histonet] Histology as art! In-Reply-To: References: Message-ID: <1403885640968.4401@accuratediagnosticlabs.com> They're actually very pretty!! I'm going to check out this museum. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Emily Brown Sent: Friday, June 27, 2014 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology as art! Hello Histonetters, I'm really looking forward to going to the brand new Morbid Anatomy Museum in NYC, but imagine my surprise when I found some histology in their online gift shop!! http://morbidanatomy.bigcartel.com/category/gifts Histology is beautiful, but it is odd to look at those images on clothing. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> luriechildrens.org Fri Jun 27 11:51:12 2014 From: NMargaryan <@t> luriechildrens.org (Margaryan, Naira) Date: Fri Jun 27 11:51:31 2014 Subject: [Histonet] Histology as art! In-Reply-To: References: Message-ID: <34B2EDA118548A4EB35D6E650345BA643216AA82@SV-EX11.childrensmemorial.org> ?Hi Emily, I don't mind if it is healthy tissue! It shows that even small cell or huge artery =all from the mother nature, don?t you think? Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Friday, June 27, 2014 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology as art! Hello Histonetters, I'm really looking forward to going to the brand new Morbid Anatomy Museum in NYC, but imagine my surprise when I found some histology in their online gift shop!! http://morbidanatomy.bigcartel.com/category/gifts Histology is beautiful, but it is odd to look at those images on clothing. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Fri Jun 27 12:14:40 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Fri Jun 27 12:14:48 2014 Subject: [Histonet] Re: Friday histology trivia Message-ID: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. It's become a game for me to spot it. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From Timothy.Morken <@t> ucsfmedctr.org Fri Jun 27 12:25:16 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jun 27 12:25:35 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> Message-ID: <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." Wow, good eyesight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, June 27, 2014 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Friday histology trivia Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. It's become a game for me to spot it. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bfoster <@t> mme1.com Fri Jun 27 12:27:05 2014 From: bfoster <@t> mme1.com (Barbara Foster) Date: Fri Jun 27 12:27:26 2014 Subject: [Histonet] Friday histology trivia Message-ID: <201406271727.s5RHRIs0026451@atl4mhob19.myregisteredsite.com> You are so right. Microscopes equal credibility. Maybe because we can actually see an image of the specimen rather than a squiggly line... or maybe because people actually sit at microscopes rather than at an impersonal control panel. Re: AO - They were part of the American Optical/Reichert-Jung Family. In 1986, they were acquired by Cambridge Instruments, which then merged with Leitz in 1991. The LEI in Leic comes from Leitz; the CA, from Cambridge. I had the privilege of being the Technical Marketing Manager for the research microscopy group, then, for a short period, product development manager for a suite that included the AO clinical line. Best regards, Barbara Foster From jqb7 <@t> cdc.gov Fri Jun 27 12:30:13 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Fri Jun 27 12:30:31 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> Message-ID: <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> Remember the episode of House where the physicians assisting House dropped some red liquid on a slide and had an immuno? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, June 27, 2014 1:25 PM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: Friday histology trivia Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." Wow, good eyesight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, June 27, 2014 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Friday histology trivia Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. It's become a game for me to spot it. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Fri Jun 27 12:53:33 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Jun 27 12:53:39 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632615738F74@LRGHEXVS1.practice.lrgh.org> The last medical show I watched was ER. Sent NBC an irritated e-mail after the episode where Dr. Wylie gave a resident a tube of blood and told her to take to the lab and wait there for the results since the lab loses everything. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Friday, June 27, 2014 1:30 PM To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia Remember the episode of House where the physicians assisting House dropped some red liquid on a slide and had an immuno? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, June 27, 2014 1:25 PM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: Friday histology trivia Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." Wow, good eyesight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, June 27, 2014 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Friday histology trivia Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. It's become a game for me to spot it. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From POWELL_SA <@t> mercer.edu Fri Jun 27 13:22:00 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Jun 27 13:22:06 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632615738F74@LRGHEXVS1.practice.lrgh.org> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> <38667E7FB77ECD4E91BFAEB8D98638632615738F74@LRGHEXVS1.practice.lrgh.org> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF97248DD@MERCERMAIL.MercerU.local> Most of you guys are too young to remember Quincy, who told his lab assistant that if he did not come up with an answer he would be demoted to the histology lab to count specimens. Never watched that show again. Good thing the writers were on the other side of the country at that time. But hey I have mellowed since then. All will agree that medical shows take license with truth and reality in view of the almighty $$$$$$$. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, June 27, 2014 1:54 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia The last medical show I watched was ER. Sent NBC an irritated e-mail after the episode where Dr. Wylie gave a resident a tube of blood and told her to take to the lab and wait there for the results since the lab loses everything. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Friday, June 27, 2014 1:30 PM To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia Remember the episode of House where the physicians assisting House dropped some red liquid on a slide and had an immuno? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, June 27, 2014 1:25 PM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: Friday histology trivia Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." Wow, good eyesight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, June 27, 2014 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Friday histology trivia Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. It's become a game for me to spot it. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Jun 27 13:24:16 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jun 27 13:24:29 2014 Subject: [Histonet] Friday histology trivia In-Reply-To: <201406271727.s5RHRIs0026451@atl4mhob19.myregisteredsite.com> References: <201406271727.s5RHRIs0026451@atl4mhob19.myregisteredsite.com> Message-ID: <761E2B5697F795489C8710BCC72141FF3678ADD0@ex07.net.ucsf.edu> " The LEI in Leic comes from Leitz; the CA, from Cambridge. " Barbara, You get the award for best trivia fact of the year! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Foster Sent: Friday, June 27, 2014 10:27 AM To: Histonet Subject: [Histonet] Friday histology trivia You are so right. Microscopes equal credibility. Maybe because we can actually see an image of the specimen rather than a squiggly line... or maybe because people actually sit at microscopes rather than at an impersonal control panel. Re: AO - They were part of the American Optical/Reichert-Jung Family. In 1986, they were acquired by Cambridge Instruments, which then merged with Leitz in 1991. The LEI in Leic comes from Leitz; the CA, from Cambridge. I had the privilege of being the Technical Marketing Manager for the research microscopy group, then, for a short period, product development manager for a suite that included the AO clinical line. Best regards, Barbara Foster _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Jun 27 13:28:31 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jun 27 13:28:37 2014 Subject: [Histonet] Histology as art! In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0D7919@evcspmbx2.ads.northwestern.edu> Love it! There is a tech that makes jewelry that sells at NSH. Made in a variety of special stains... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Friday, June 27, 2014 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology as art! Hello Histonetters, I'm really looking forward to going to the brand new Morbid Anatomy Museum in NYC, but imagine my surprise when I found some histology in their online gift shop!! http://morbidanatomy.bigcartel.com/category/gifts Histology is beautiful, but it is odd to look at those images on clothing. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Fri Jun 27 13:34:04 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Jun 27 13:34:09 2014 Subject: [Histonet] Histology as art! In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF2F0D7919@evcspmbx2.ads.northwestern.edu> References: <62C639732D3F274DACED033EBDF6ADAF2F0D7919@evcspmbx2.ads.northwestern.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF97248EB@MERCERMAIL.MercerU.local> That would be Mequita Praet, does a beautiful job. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Friday, June 27, 2014 2:29 PM To: Emily Brown; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology as art! Love it! There is a tech that makes jewelry that sells at NSH. Made in a variety of special stains... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Friday, June 27, 2014 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology as art! Hello Histonetters, I'm really looking forward to going to the brand new Morbid Anatomy Museum in NYC, but imagine my surprise when I found some histology in their online gift shop!! http://morbidanatomy.bigcartel.com/category/gifts Histology is beautiful, but it is odd to look at those images on clothing. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From udsd007 <@t> gmail.com Fri Jun 27 13:35:14 2014 From: udsd007 <@t> gmail.com (Mike Andrews) Date: Fri Jun 27 13:35:22 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> Message-ID: One of my current is the (Salonpas?) ad I which there is a very obvious AO Series 10 just behind the presenter. Good old brass'n'glass, but hardly current -- even if I do own a few. Mike Andrews, W5EGO WWME Oklahoma area executive team > On Jun 27, 2014, at 12:25 PM, "Morken, Timothy" wrote: > > Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." > > Wow, good eyesight! > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson > Sent: Friday, June 27, 2014 10:15 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Friday histology trivia > > Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. > > Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. > It's become a game for me to spot it. > > Teri Johnson > Manager, Histology > Genomics Institute for > Novartis Research > Foundation > San Diego, CA > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wsimons <@t> athensgastro.com Fri Jun 27 13:38:25 2014 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Fri Jun 27 13:38:30 2014 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUkU6IFJlOiBGcmlkYXkgaGlzdG9sb2d5IHRyaXZpYQ==?= In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25BF97248DD@MERCERMAIL.MercerU.local> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> <38667E7FB77ECD4E91BFAEB8D98638632615738F74@LRGHEXVS1.practice.lrgh.org> <9BF995BC0E47744E9673A41486E24EE25BF97248DD@MERCERMAIL.MercerU.local> Message-ID: <20140627183825.7666.qmail@server276.com> Quincy made derogatory remarks that anyone could do what Sam was doing. Then a few of us complained and the next few episodes promoted us. I think I'll talk to my walking ATL connections and see if we can get histotechs back in the limelight. Maybe on "walking dead"? Wanda > -------Original Message------- > From: Shirley A. Powell > To: Podawiltz, Thomas , Sanders, Jeanine (CDC/OID/NCEZID) , 'Morken, Timothy' , 'Teri Johnson' , 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Re: Friday histology trivia > Sent: Jun 27 '14 14:22 > > Most of you guys are too young to remember Quincy, who told his lab assistant that if he did not come up with an answer he would be demoted to the histology lab to count specimens.??Never watched that show again.??Good thing the writers were on the other side of the country at that time.??But hey I have mellowed since then.??All will agree that medical shows take license with truth and reality in view of the almighty $$$$$$$.?? > > Shirley?? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas > Sent: Friday, June 27, 2014 1:54 PM > To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Re: Friday histology trivia > > The last medical show I watched was ER. Sent NBC an irritated e-mail after the episode where Dr. Wylie gave a resident a tube of blood and told her to take to the lab and wait there for the results since the lab loses everything. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) > Sent: Friday, June 27, 2014 1:30 PM > To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Re: Friday histology trivia > > Remember the episode of House where the physicians assisting House dropped some red liquid on a slide and had an immuno? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Friday, June 27, 2014 1:25 PM > To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Re: Friday histology trivia > > Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." > > Wow, good eyesight! > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson > Sent: Friday, June 27, 2014 10:15 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Friday histology trivia > > Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. > > Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. > It's become a game for me to spot it. > > Teri Johnson > Manager, Histology > Genomics Institute for > Novartis Research > Foundation > San Diego, CA > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL.?? > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.??If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mjones <@t> metropath.com Fri Jun 27 13:39:13 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Fri Jun 27 13:39:19 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> Message-ID: Ha! I can beat that~ we have a cryostat that is at least 50+ years old, old AO microtome cryostat. Still works, barely. . .looks just like the old fashion ice-cream holders. (P.S. Prob not true to life, but I liked Quincy-did I just date myself?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/27/14, 12:35 PM, "Mike Andrews" wrote: >One of my current is the (Salonpas?) ad I which there is a very obvious >AO Series 10 just behind the presenter. Good old brass'n'glass, but >hardly current -- even if I do own a few. > >Mike Andrews, W5EGO >WWME Oklahoma area executive team > >> On Jun 27, 2014, at 12:25 PM, "Morken, Timothy" >> wrote: >> >> Are all news stories are as faked as the ones showing something in a >>lab? One had two doctors(?) in lab coats peering at a microscope slide >>they are holding to the light above their head and the reporter is >>saying "they are examining samples from a cancer patient." >> >> Wow, good eyesight! >> >> Tim Morken >> Supervisor, Electron Microscopy and Neuromuscular Special Studies >> UC San Francisco Medical Center >> San Francisco, CA >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri >>Johnson >> Sent: Friday, June 27, 2014 10:15 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Re: Friday histology trivia >> >> Carefully placed scientific equipment, as well as spokespeople dressed >>in lab coats, are great marketing tools for anything touted to cure us >>or make us healthier. >> >> Next time you watch an actual medical or research-based news story, >>check out how they ALWAYS show someone pipetting. Always. >> It's become a game for me to spot it. >> >> Teri Johnson >> Manager, Histology >> Genomics Institute for >> Novartis Research >> Foundation >> San Diego, CA >> 858-332-4752 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Fri Jun 27 13:40:45 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Jun 27 13:40:49 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF97248F7@MERCERMAIL.MercerU.local> Yep you may be old as me. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Friday, June 27, 2014 2:39 PM To: Mike Andrews; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Re: Friday histology trivia Ha! I can beat that~ we have a cryostat that is at least 50+ years old, old AO microtome cryostat. Still works, barely. . .looks just like the old fashion ice-cream holders. (P.S. Prob not true to life, but I liked Quincy-did I just date myself?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/27/14, 12:35 PM, "Mike Andrews" wrote: >One of my current is the (Salonpas?) ad I which there is a very obvious >AO Series 10 just behind the presenter. Good old brass'n'glass, but >hardly current -- even if I do own a few. > >Mike Andrews, W5EGO >WWME Oklahoma area executive team > >> On Jun 27, 2014, at 12:25 PM, "Morken, Timothy" >> wrote: >> >> Are all news stories are as faked as the ones showing something in a >>lab? One had two doctors(?) in lab coats peering at a microscope slide >>they are holding to the light above their head and the reporter is >>saying "they are examining samples from a cancer patient." >> >> Wow, good eyesight! >> >> Tim Morken >> Supervisor, Electron Microscopy and Neuromuscular Special Studies UC >> San Francisco Medical Center San Francisco, CA >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri >>Johnson >> Sent: Friday, June 27, 2014 10:15 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Re: Friday histology trivia >> >> Carefully placed scientific equipment, as well as spokespeople >>dressed in lab coats, are great marketing tools for anything touted to >>cure us or make us healthier. >> >> Next time you watch an actual medical or research-based news story, >>check out how they ALWAYS show someone pipetting. Always. >> It's become a game for me to spot it. >> >> Teri Johnson >> Manager, Histology >> Genomics Institute for >> Novartis Research >> Foundation >> San Diego, CA >> 858-332-4752 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Fri Jun 27 13:41:28 2014 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Jun 27 13:41:51 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: <20140627183825.7666.qmail@server276.com> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> <38667E7FB77ECD4E91BFAEB8D98638632615738F74@LRGHEXVS1.practice.lrgh.org> <9BF995BC0E47744E9673A41486E24EE25BF97248DD@MERCERMAIL.MercerU.local> <20140627183825.7666.qmail@server276.com> Message-ID: I'm a histotech on Friday... I AM the walking dead :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wsimons@athensgastro.com Sent: Friday, June 27, 2014 1:38 PM To: Shirley A. Powell; Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] RE: Re: Friday histology trivia Quincy made derogatory remarks that anyone could do what Sam was doing. Then a few of us complained and the next few episodes promoted us. I think I'll talk to my walking ATL connections and see if we can get histotechs back in the limelight. Maybe on "walking dead"? Wanda > -------Original Message------- > From: Shirley A. Powell > To: Podawiltz, Thomas , Sanders, Jeanine > (CDC/OID/NCEZID) , 'Morken, Timothy' > , 'Teri Johnson' , > 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] RE: Re: Friday histology trivia > Sent: Jun 27 '14 14:22 > > Most of you guys are too young to remember Quincy, who told his lab > assistant that if he did not come up with an answer he would be demoted to the histology lab to count specimens.??Never watched that show again.??Good thing the writers were on the other side of the country at that time.??But hey I have mellowed since then.??All will agree that medical shows take license with truth and reality in view of the almighty $$$$$$$. > > Shirley > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bou > nces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E% > 2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6 > N2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=9b5abfcb235a2a3dbd25b16e0c27819a05 > 2d4fe65d3d8814c4fde009dcfb9674] On Behalf Of Podawiltz, Thomas > Sent: Friday, June 27, 2014 1:54 PM > To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Re: Friday histology trivia > > The last medical show I watched was ER. Sent NBC an irritated e-mail after the episode where Dr. Wylie gave a resident a tube of blood and told her to take to the lab and wait there for the results since the lab loses everything. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bou > nces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E% > 2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6 > N2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=9b5abfcb235a2a3dbd25b16e0c27819a05 > 2d4fe65d3d8814c4fde009dcfb9674] On Behalf Of Sanders, Jeanine > (CDC/OID/NCEZID) > Sent: Friday, June 27, 2014 1:30 PM > To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Re: Friday histology trivia > > Remember the episode of House where the physicians assisting House dropped some red liquid on a slide and had an immuno? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bou > nces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E% > 2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6 > N2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=9b5abfcb235a2a3dbd25b16e0c27819a05 > 2d4fe65d3d8814c4fde009dcfb9674] On Behalf Of Morken, Timothy > Sent: Friday, June 27, 2014 1:25 PM > To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Re: Friday histology trivia > > Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." > > Wow, good eyesight! > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies UC > San Francisco Medical Center San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bou > nces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E% > 2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6 > N2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=9b5abfcb235a2a3dbd25b16e0c27819a05 > 2d4fe65d3d8814c4fde009dcfb9674] On Behalf Of Teri Johnson > Sent: Friday, June 27, 2014 10:15 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Friday histology trivia > > Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. > > Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. > It's become a game for me to spot it. > > Teri Johnson > Manager, Histology > Genomics Institute for > Novartis Research > Foundation > San Diego, CA > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern > .edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2 > BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6N > 2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=99a6a55bcb53e1d6da9165183ddd7711293 > b27e3f07c9e1bb36b1a2d9f932f81 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern > .edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2 > BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6N > 2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=99a6a55bcb53e1d6da9165183ddd7711293 > b27e3f07c9e1bb36b1a2d9f932f81 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern > .edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2 > BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6N > 2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=99a6a55bcb53e1d6da9165183ddd7711293 > b27e3f07c9e1bb36b1a2d9f932f81 > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.??If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern > .edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2 > BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6N > 2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=99a6a55bcb53e1d6da9165183ddd7711293 > b27e3f07c9e1bb36b1a2d9f932f81 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern > .edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2 > BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6N > 2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=99a6a55bcb53e1d6da9165183ddd7711293 > b27e3f07c9e1bb36b1a2d9f932f81 > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=vtu0VH89zYQtSQC6N2EZqfbxEo0zQtgbnMuGLc7e35k%3D%0A&s=99a6a55bcb53e1d6da9165183ddd7711293b27e3f07c9e1bb36b1a2d9f932f81 This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From billodonnell <@t> catholichealth.net Fri Jun 27 13:43:15 2014 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Jun 27 13:43:21 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> Message-ID: HA! I can beat that... I still have to cut ice from the river in the winter and store it in an ice house so we can do frozen! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Friday, June 27, 2014 1:39 PM To: Mike Andrews; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Re: Friday histology trivia Ha! I can beat that~ we have a cryostat that is at least 50+ years old, old AO microtome cryostat. Still works, barely. . .looks just like the old fashion ice-cream holders. (P.S. Prob not true to life, but I liked Quincy-did I just date myself?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 6/27/14, 12:35 PM, "Mike Andrews" wrote: >One of my current is the (Salonpas?) ad I which there is a very obvious >AO Series 10 just behind the presenter. Good old brass'n'glass, but >hardly current -- even if I do own a few. > >Mike Andrews, W5EGO >WWME Oklahoma area executive team > >> On Jun 27, 2014, at 12:25 PM, "Morken, Timothy" >> wrote: >> >> Are all news stories are as faked as the ones showing something in a >>lab? One had two doctors(?) in lab coats peering at a microscope slide >>they are holding to the light above their head and the reporter is >>saying "they are examining samples from a cancer patient." >> >> Wow, good eyesight! >> >> Tim Morken >> Supervisor, Electron Microscopy and Neuromuscular Special Studies UC >> San Francisco Medical Center San Francisco, CA >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >>[https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bou >>nces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E% >>2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=eXbUzrOr%2FQWi5a >>QG405csuQNWg5kvOQTM4Bj96gYc1E%3D%0A&s=1bebcb7c118636c237f047b15590050d >>01822bed37cb5a83cbd76d3fd0d1b6e7] On Behalf Of Teri Johnson >> Sent: Friday, June 27, 2014 10:15 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Re: Friday histology trivia >> >> Carefully placed scientific equipment, as well as spokespeople >>dressed in lab coats, are great marketing tools for anything touted to >>cure us or make us healthier. >> >> Next time you watch an actual medical or research-based news story, >>check out how they ALWAYS show someone pipetting. Always. >> It's become a game for me to spot it. >> >> Teri Johnson >> Manager, Histology >> Genomics Institute for >> Novartis Research >> Foundation >> San Diego, CA >> 858-332-4752 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwester >> n.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E >> %2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=eXbUzrOr%2FQWi >> 5aQG405csuQNWg5kvOQTM4Bj96gYc1E%3D%0A&s=f4881f0a77bcc67596c4e8d0209ba >> 4b9bdd2eeec549a4b9b7e4914d6528b4860 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwester >> n.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E >> %2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=eXbUzrOr%2FQWi >> 5aQG405csuQNWg5kvOQTM4Bj96gYc1E%3D%0A&s=f4881f0a77bcc67596c4e8d0209ba >> 4b9bdd2eeec549a4b9b7e4914d6528b4860 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern. >edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BU >K3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=eXbUzrOr%2FQWi5aQG40 >5csuQNWg5kvOQTM4Bj96gYc1E%3D%0A&s=f4881f0a77bcc67596c4e8d0209ba4b9bdd2e >eec549a4b9b7e4914d6528b4860 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=eXbUzrOr%2FQWi5aQG405csuQNWg5kvOQTM4Bj96gYc1E%3D%0A&s=f4881f0a77bcc67596c4e8d0209ba4b9bdd2eeec549a4b9b7e4914d6528b4860 This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From mturner <@t> CSILaboratories.com Fri Jun 27 13:44:50 2014 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Fri Jun 27 13:45:16 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: <20140627183825.7666.qmail@server276.com> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> <38667E7FB77ECD4E91BFAEB8D98638632615738F74@LRGHEXVS1.practice.lrgh.org> <9BF995BC0E47744E9673A41486E24EE25BF97248DD@MERCERMAIL.MercerU.local> <20140627183825.7666.qmail@server276.com> Message-ID: <643626B74DE2814D8537057F40E1A10B0A35B384@CSI-MX-NODEA.CSI-LABS.local> Count me in, Wanda! After some of the long hours I work, I probably wouldn't need makeup! Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wsimons@athensgastro.com Sent: Friday, June 27, 2014 2:38 PM To: Shirley A. Powell; Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] RE: Re: Friday histology trivia Quincy made derogatory remarks that anyone could do what Sam was doing. Then a few of us complained and the next few episodes promoted us. I think I'll talk to my walking ATL connections and see if we can get histotechs back in the limelight. Maybe on "walking dead"? Wanda > -------Original Message------- > From: Shirley A. Powell > To: Podawiltz, Thomas , Sanders, Jeanine > (CDC/OID/NCEZID) , 'Morken, Timothy' > , 'Teri Johnson' , > 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] RE: Re: Friday histology trivia > Sent: Jun 27 '14 14:22 > > Most of you guys are too young to remember Quincy, who told his lab > assistant that if he did not come up with an answer he would be demoted to the histology lab to count specimens.??Never watched that show again.??Good thing the writers were on the other side of the country at that time.??But hey I have mellowed since then.??All will agree that medical shows take license with truth and reality in view of the almighty $$$$$$$. > > Shirley > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Podawiltz, Thomas > Sent: Friday, June 27, 2014 1:54 PM > To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Re: Friday histology trivia > > The last medical show I watched was ER. Sent NBC an irritated e-mail after the episode where Dr. Wylie gave a resident a tube of blood and told her to take to the lab and wait there for the results since the lab loses everything. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Sanders, Jeanine (CDC/OID/NCEZID) > Sent: Friday, June 27, 2014 1:30 PM > To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Re: Friday histology trivia > > Remember the episode of House where the physicians assisting House dropped some red liquid on a slide and had an immuno? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Morken, Timothy > Sent: Friday, June 27, 2014 1:25 PM > To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Re: Friday histology trivia > > Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." > > Wow, good eyesight! > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies UC > San Francisco Medical Center San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri > Johnson > Sent: Friday, June 27, 2014 10:15 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Friday histology trivia > > Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. > > Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. > It's become a game for me to spot it. > > Teri Johnson > Manager, Histology > Genomics Institute for > Novartis Research > Foundation > San Diego, CA > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.??If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Fri Jun 27 14:11:21 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Jun 27 14:11:27 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25BF97248DD@MERCERMAIL.MercerU.local> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> <38667E7FB77ECD4E91BFAEB8D98638632615738F74@LRGHEXVS1.practice.lrgh.org> <9BF995BC0E47744E9673A41486E24EE25BF97248DD@MERCERMAIL.MercerU.local> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632615738F7B@LRGHEXVS1.practice.lrgh.org> The navy lab I was stationed at in VA, we had a picture of Quincy on our dart board. Yeah, he was our favorite target. Tom -----Original Message----- From: Shirley A. Powell [mailto:POWELL_SA@mercer.edu] Sent: Friday, June 27, 2014 2:22 PM To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: Re: Friday histology trivia Most of you guys are too young to remember Quincy, who told his lab assistant that if he did not come up with an answer he would be demoted to the histology lab to count specimens. Never watched that show again. Good thing the writers were on the other side of the country at that time. But hey I have mellowed since then. All will agree that medical shows take license with truth and reality in view of the almighty $$$$$$$. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, June 27, 2014 1:54 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia The last medical show I watched was ER. Sent NBC an irritated e-mail after the episode where Dr. Wylie gave a resident a tube of blood and told her to take to the lab and wait there for the results since the lab loses everything. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Friday, June 27, 2014 1:30 PM To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia Remember the episode of House where the physicians assisting House dropped some red liquid on a slide and had an immuno? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, June 27, 2014 1:25 PM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: Friday histology trivia Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." Wow, good eyesight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Friday, June 27, 2014 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Friday histology trivia Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. It's become a game for me to spot it. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From shive003 <@t> umn.edu Fri Jun 27 14:28:24 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Jun 27 14:28:35 2014 Subject: [Histonet] Friday histology trivia Message-ID: The Ki in Ki-67 is from the city of origin (Kiel, Germany), and is really pronounced KEY-67 (not K. I. 67). The 67 in Ki-67 is from the original clone in a 96-well plate. Happy Friday! -- Jan Shivers Senior Scientist University of Minnesota On Fri, Jun 27, 2014 at 1:24 PM, Morken, Timothy < Timothy.Morken@ucsfmedctr.org> wrote: > " The LEI in Leic comes from Leitz; the CA, from Cambridge. " > > Barbara, You get the award for best trivia fact of the year! > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Foster > Sent: Friday, June 27, 2014 10:27 AM > To: Histonet > Subject: [Histonet] Friday histology trivia > > You are so right. Microscopes equal credibility. Maybe because we can > actually see an image of the specimen rather than a squiggly line... or > maybe because people actually sit at microscopes rather than at an > impersonal control panel. > > Re: AO - They were part of the American Optical/Reichert-Jung Family. In > 1986, they were acquired by Cambridge Instruments, which then merged with > Leitz in 1991. The LEI in Leic comes from Leitz; the CA, from Cambridge. > I had the privilege of being the Technical Marketing Manager for the > research microscopy group, then, for a short period, product development > manager for a suite that included the AO clinical line. > > Best regards, > Barbara Foster > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jonathan.Arzt <@t> ARS.USDA.GOV Fri Jun 27 15:00:14 2014 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Fri Jun 27 15:00:46 2014 Subject: [Histonet] Plum Island Veterinary Histotechnologist Job Message-ID: <7213AC15544DE945843D821E1769E38A06800C38@001FSN2MPN1-053.001f.mgd2.msft.net> A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old Saybrook, CT. Workload is centered on veterinary viral pathogenesis research; emphasis is foot-and-mouth disease and African swine fever. Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400 Salary $44,175.00 to $84,990.00 / per year commensurate with experience. Federal benefits package. This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. From billodonnell <@t> catholichealth.net Fri Jun 27 15:09:56 2014 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Jun 27 15:10:14 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632615738F7B@LRGHEXVS1.practice.lrgh.org> References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> <38667E7FB77ECD4E91BFAEB8D98638632615738F74@LRGHEXVS1.practice.lrgh.org> <9BF995BC0E47744E9673A41486E24EE25BF97248DD@MERCERMAIL.MercerU.local> <38667E7FB77ECD4E91BFAEB8D98638632615738F7B@LRGHEXVS1.practice.lrgh.org> Message-ID: Second favorite - don't forget Roger! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, June 27, 2014 2:11 PM To: Shirley A. Powell; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia The navy lab I was stationed at in VA, we had a picture of Quincy on our dart board. Yeah, he was our favorite target. Tom -----Original Message----- From: Shirley A. Powell [https://urldefense.proofpoint.com/v1/url?u=http://mailto:POWELL_SA%40mercer.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=760f9835534c28a8d5a039ae8d8ca02cad445502b7e1ceb7dfe700a3d4a471e4] Sent: Friday, June 27, 2014 2:22 PM To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: Re: Friday histology trivia Most of you guys are too young to remember Quincy, who told his lab assistant that if he did not come up with an answer he would be demoted to the histology lab to count specimens. Never watched that show again. Good thing the writers were on the other side of the country at that time. But hey I have mellowed since then. All will agree that medical shows take license with truth and reality in view of the almighty $$$$$$$. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192] On Behalf Of Podawiltz, Thomas Sent: Friday, June 27, 2014 1:54 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia The last medical show I watched was ER. Sent NBC an irritated e-mail after the episode where Dr. Wylie gave a resident a tube of blood and told her to take to the lab and wait there for the results since the lab loses everything. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Friday, June 27, 2014 1:30 PM To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia Remember the episode of House where the physicians assisting House dropped some red liquid on a slide and had an immuno? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192] On Behalf Of Morken, Timothy Sent: Friday, June 27, 2014 1:25 PM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: Friday histology trivia Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." Wow, good eyesight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192] On Behalf Of Teri Johnson Sent: Friday, June 27, 2014 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Friday histology trivia Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. It's become a game for me to spot it. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 THIS MESSAGE IS CONFIDENTIAL. 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Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From tpodawiltz <@t> lrgh.org Fri Jun 27 15:11:25 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Jun 27 15:11:33 2014 Subject: [Histonet] RE: Re: Friday histology trivia In-Reply-To: References: <9F3CFEE76E51B64991C7485270890B40498DF8F1@EX5.lj.gnf.org> <761E2B5697F795489C8710BCC72141FF3678AD91@ex07.net.ucsf.edu> <3B2CD438E1628A41BD687E98B963B7811FDB9FD5@EMBX-CHAM2.cdc.gov> <38667E7FB77ECD4E91BFAEB8D98638632615738F74@LRGHEXVS1.practice.lrgh.org> <9BF995BC0E47744E9673A41486E24EE25BF97248DD@MERCERMAIL.MercerU.local> <38667E7FB77ECD4E91BFAEB8D98638632615738F7B@LRGHEXVS1.practice.lrgh.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632615738F7F@LRGHEXVS1.practice.lrgh.org> Oh man, how can I forget. Microtome grease and his cover's head band. Tom -----Original Message----- From: O'Donnell, Bill [mailto:billodonnell@catholichealth.net] Sent: Friday, June 27, 2014 4:10 PM To: Podawiltz, Thomas; Shirley A. Powell; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: Re: Friday histology trivia Second favorite - don't forget Roger! - Bill -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, June 27, 2014 2:11 PM To: Shirley A. Powell; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia The navy lab I was stationed at in VA, we had a picture of Quincy on our dart board. Yeah, he was our favorite target. Tom -----Original Message----- From: Shirley A. Powell [https://urldefense.proofpoint.com/v1/url?u=http://mailto:POWELL_SA%40mercer.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=760f9835534c28a8d5a039ae8d8ca02cad445502b7e1ceb7dfe700a3d4a471e4] Sent: Friday, June 27, 2014 2:22 PM To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: Re: Friday histology trivia Most of you guys are too young to remember Quincy, who told his lab assistant that if he did not come up with an answer he would be demoted to the histology lab to count specimens. Never watched that show again. Good thing the writers were on the other side of the country at that time. But hey I have mellowed since then. All will agree that medical shows take license with truth and reality in view of the almighty $$$$$$$. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192] On Behalf Of Podawiltz, Thomas Sent: Friday, June 27, 2014 1:54 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia The last medical show I watched was ER. Sent NBC an irritated e-mail after the episode where Dr. Wylie gave a resident a tube of blood and told her to take to the lab and wait there for the results since the lab loses everything. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Friday, June 27, 2014 1:30 PM To: 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Re: Friday histology trivia Remember the episode of House where the physicians assisting House dropped some red liquid on a slide and had an immuno? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192] On Behalf Of Morken, Timothy Sent: Friday, June 27, 2014 1:25 PM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re: Friday histology trivia Are all news stories are as faked as the ones showing something in a lab? One had two doctors(?) in lab coats peering at a microscope slide they are holding to the light above their head and the reporter is saying "they are examining samples from a cancer patient." Wow, good eyesight! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=e0366327209d09783810b3f56489aa120062fa2a8c34700228309c97db308192] On Behalf Of Teri Johnson Sent: Friday, June 27, 2014 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Friday histology trivia Carefully placed scientific equipment, as well as spokespeople dressed in lab coats, are great marketing tools for anything touted to cure us or make us healthier. Next time you watch an actual medical or research-based news story, check out how they ALWAYS show someone pipetting. Always. It's become a game for me to spot it. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 THIS MESSAGE IS CONFIDENTIAL. 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Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=biEQ%2FFHsVzR%2FBHuSEm3zf4GZDI9tsuPiOG2wNEw%2FJuo%3D%0A&s=23d40a43d89f7f3a1896a0d6403a56d9c8347253680af6e73cac81fafb165bd4 This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From akemiat3377 <@t> gmail.com Fri Jun 27 15:14:14 2014 From: akemiat3377 <@t> gmail.com (Eileen Akemi Allison) Date: Fri Jun 27 15:14:20 2014 Subject: [Histonet] Friday histology trivia In-Reply-To: References: Message-ID: <8A6194CC-00AE-48C8-8162-726EE4AC6E72@gmail.com> Glad to know I was correct all these years! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aallison@montereygi.com Tele: (831) 375-3577 X117 On Jun 27, 2014, at 12:28 PM, Jan Shivers wrote: > The Ki in Ki-67 is from the city of origin (Kiel, Germany), and is really > pronounced KEY-67 (not K. I. 67). > > The 67 in Ki-67 is from the original clone in a 96-well plate. > > Happy Friday! > -- > Jan Shivers > Senior Scientist > University of Minnesota > > > On Fri, Jun 27, 2014 at 1:24 PM, Morken, Timothy < > Timothy.Morken@ucsfmedctr.org> wrote: > >> " The LEI in Leic comes from Leitz; the CA, from Cambridge. " >> >> Barbara, You get the award for best trivia fact of the year! >> >> >> >> Tim Morken >> Supervisor, Electron Microscopy and Neuromuscular Special Studies >> UC San Francisco Medical Center >> San Francisco, CA >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Foster >> Sent: Friday, June 27, 2014 10:27 AM >> To: Histonet >> Subject: [Histonet] Friday histology trivia >> >> You are so right. Microscopes equal credibility. Maybe because we can >> actually see an image of the specimen rather than a squiggly line... or >> maybe because people actually sit at microscopes rather than at an >> impersonal control panel. >> >> Re: AO - They were part of the American Optical/Reichert-Jung Family. In >> 1986, they were acquired by Cambridge Instruments, which then merged with >> Leitz in 1991. The LEI in Leic comes from Leitz; the CA, from Cambridge. >> I had the privilege of being the Technical Marketing Manager for the >> research microscopy group, then, for a short period, product development >> manager for a suite that included the AO clinical line. >> >> Best regards, >> Barbara Foster >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Luis.Chiriboga <@t> nyumc.org Fri Jun 27 15:29:56 2014 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Fri Jun 27 15:30:02 2014 Subject: [Histonet] RE: BrDU In-Reply-To: <3E6798F00C9F494399E96B720ECD14292B2A54@MSGWCDCPMB22.nyumc.org> References: <3E6798F00C9F494399E96B720ECD14292B2982@MSGWCDCPMB22.nyumc.org> <3E6798F00C9F494399E96B720ECD14292B2A54@MSGWCDCPMB22.nyumc.org> Message-ID: <3E6798F00C9F494399E96B720ECD14292B82B2@MSGWCDCPMB22.nyumc.org> Thanks for all the quick responses everyone Good weekend!! -----Original Message----- From: Chiriboga, Luis Sent: Tuesday, June 24, 2014 11:55 AM To: (Histonet@lists.utsouthwestern.edu) Subject: RE: BrDU Apologies, I meant to add for FFPE muse/rat tissue TX's -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Tuesday, June 24, 2014 11:12 AM To: (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] BrDU Hi Histonetters Can anyone recommend a good anti-BrDU antibody and protocol Thanks & Happy Summer!! ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From TNMayer <@t> mdanderson.org Mon Jun 30 07:32:08 2014 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Jun 30 07:33:33 2014 Subject: [Histonet] RE: Histology trivia Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B3BA97D@D1PWPEXMBX05.mdanderson.edu> I remember an episode of CSI where the ME had a bowl of ice next to the body where he was doing an autopsy and cut the tissue right there. A year after that the star did a series of promos for Lab week. I guess the techs got on them about the scene. They did however, show the sections being cut on an old AO (black of course), and the ME did the staining. As a kid I loved Quincy, and it got me interested in science. When I talk to the baby boomers about my work, I do refer to Sam in the show. House had his interns cut and stain the tissue slides (go figure) with an AFB once. Ridiculous!! Sincerely, Toysha N. Mayer, MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 1 Date: Fri, 27 Jun 2014 18:44:50 +0000 From: Mark Turner Subject: RE: [Histonet] RE: Re: Friday histology trivia To: "wsimons@athensgastro.com" , "Shirley A. Powell" , "Podawiltz, Thomas" , "Sanders, Jeanine (CDC/OID/NCEZID)" , "'Morken, Timothy'" , 'Teri Johnson' , "'histonet@lists.utsouthwestern.edu'" Message-ID: <643626B74DE2814D8537057F40E1A10B0A35B384@CSI-MX-NODEA.CSI-LABS.local> Content-Type: text/plain; charset="utf-8" Count me in, Wanda! After some of the long hours I work, I probably wouldn't need makeup! Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wsimons@athensgastro.com Sent: Friday, June 27, 2014 2:38 PM To: Shirley A. Powell; Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); 'Morken, Timothy'; 'Teri Johnson'; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] RE: Re: Friday histology trivia Quincy made derogatory remarks that anyone could do what Sam was doing. Then a few of us complained and the next few episodes promoted us. I think I'll talk to my walking ATL connections and see if we can get histotechs back in the limelight. Maybe on "walking dead"? Wanda > -------Original Message------- > From: Shirley A. Powell > To: Podawiltz, Thomas , Sanders, Jeanine > (CDC/OID/NCEZID) , 'Morken, Timothy' > , 'Teri Johnson' , > 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] RE: Re: Friday histology trivia > Sent: Jun 27 '14 14:22 > > Most of you guys are too young to remember Quincy, who told his lab > assistant that if he did not come up with an answer he would be demoted to the histology lab to count specimens.??Never watched that show again.??Good thing the writers were on the other side of the country at that time.??But hey I have mellowed since then.??All will agree that medical shows take license with truth and reality in view of the almighty $$$$$$$. > > Shirley > From Jonathan.Arzt <@t> ARS.USDA.GOV Mon Jun 30 22:32:35 2014 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Mon Jun 30 22:32:56 2014 Subject: [Histonet] Veterinary Histotechnologist Job at Plum Island USDA Message-ID: <7213AC15544DE945843D821E1769E38A068090D4@001FSN2MPN1-052.001f.mgd2.msft.net> A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old Saybrook, CT. Workload is centered on veterinary viral pathogenesis research; emphasis is foot-and-mouth disease and African swine fever. Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400 Salary $44,175.00 to $84,990.00 / per year commensurate with experience. Federal benefits package. This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately.