[Histonet] RE: Storage of HER2 neu control slides

[Sebree Linda A]

Thanks Joelle.

I'm surprised that Oracle is suggesting overnight baking as I noticed a huge difference when I dried some test tissue controls for 30" @ 60 degrees vs 10" @ 60 degrees.  I was testing some blocks that I had 3 cores  of varying degrees of intensity.  All showed much decreased reactivity at 30" vs 10".  In fact, the 2 weaker cores were virtually negative.  I can only assume you're using a different clone than we are and that there is different epitope stability between clones.

Thanks for your response,

Linda


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

From: Joelle Weaver [mailto:joelleweaver <@t> hotmail.com]
Sent: Monday, July 14, 2014 9:25 AM
To: Cartun, Richard; Sebree Linda A; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Storage of HER2 neu control slides

I do the complete drain & dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often.


Joelle Weaver MAOM, HTL (ASCP) QIHC





> From: Richard.Cartun <@t> hhchealth.org<mailto:Richard.Cartun <@t> hhchealth.org>
> To: LSebree <@t> uwhealth.org<mailto:LSebree <@t> uwhealth.org>; Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
> Date: Mon, 14 Jul 2014 14:22:43 +0000
> CC:
> Subject: [Histonet] RE: Storage of HER2 neu control slides
>
> We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 972-1596 Office
> (860) 545-2204 Fax
> richard.cartun <@t> hhchealth.org<mailto:richard.cartun <@t> hhchealth.org>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
> Sent: Monday, July 14, 2014 8:44 AM
> To: Histonet (Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>)
> Subject: [Histonet] Storage of HER2 neu control slides
>
> Good morning,
>
> I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides?
>
> Thanks for any and all replies,
>
> Linda
>
>
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
>
>
>
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