From relia1 <@t> earthlink.net Tue Jul 1 09:38:22 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jul 1 09:38:32 2014 Subject: [Histonet] RELIA Hot Histology Job Alert and A Special Treat for your 4th of July parties and cookouts!! 7-1-2014 Message-ID: <00d801cf953a$1cfcb900$56f62b00$@earthlink.net> Hi Histonetters!! I hope everybody is having a great week and looking forward to a Fun-filled, Safe and Happy 4th of July Weekend. In addition to some exciting new Job Opportunities I have a Fun Idea to Share for Pool Parties And Cookouts. Sprinkle Red Pop Rocks And Blue Sprinkles On Iced Sugar Cookies To Make Edible Firecrackers!! AND.. Here are the exciting new opportunities that have come in this week that I want to pass along as well. Maybe they will look good to you or someone you know. Brand New Hot Histology Opportunities!! Lead Mohs Histotech - Brand New Lab Waco, TX Lead Dermpath Histotech - Dallas, TX Lead Dermpath Histotech - Chicago, IL Histotechnician - Chicago, IL $3000.00 Sign-on bonus!!!! Histotechnician - Madison, WI RELIA's Other Exciting Opportunities Include: IHC/QC Manager - San Francisco Bay Area AP Manager - Richmond, VA Histotechnologist - Modesto, CA Histotechnologist - Belleville, IL Histotechnician - Nashville, TN IHC Supervisor - Dallas, TX All of these jobs are full time permanent positions with some of the finest facilities in the country. Most of these are RELIA exclusives that you won't see anywhere else!! My clients offer excellent compensation and benefits and most offer relocation assistance or sign- on bonuses. For more information for you or a friend please call me at the office at 866-607-3542 or on my cell at 407-353-5070 or shoot me at email at relia1@earthlink.net Have a Safe and Happy 4th of July!! Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From TanyaAbbott <@t> catholichealth.net Tue Jul 1 09:46:19 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Tue Jul 1 09:47:38 2014 Subject: [Histonet] Gram stain Message-ID: <852F7D2C14FB464D80E182B15DB138AF306AA0DE@CHIEX005.CHI.catholichealth.net> We only perform a few Gram stains a year, hardly seems worth It to keep a kit and expire reagents! Does anyone have an idea for other options? Ie, doing the stains in Micro? Checking with their BD rep to see if we can do their Gram stain kit on formalin fixed tissue,....... Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From algranth <@t> email.arizona.edu Tue Jul 1 10:10:52 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Jul 1 10:10:56 2014 Subject: [Histonet] Gram stain In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF306AA0DE@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF306AA0DE@CHIEX005.CHI.catholichealth.net> Message-ID: <12E7FAC3-2B74-4B7C-BD20-836268FAE590@email.arizona.edu> I do a gram stain but also not very often. I have used a PolyScientific kit and it has a pretty good shelf life but before that I was just making up the stains and solutions in small quantities. They aren't hard to make and I inherited a wealth of stains and chemicals here that it seems a shame not to take advantage of. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From HornHV <@t> archildrens.org Tue Jul 1 10:14:06 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Jul 1 10:14:11 2014 Subject: [Histonet] dissecting microscope Message-ID: <25A4DE08332B19499904459F00AAACB719DE6DEECE@EVS1.archildrens.org> We are looking for a dissecting microscope. Nothing real fancy and it does not have to be new. Thanks. Please email me and I will call you. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From tmcampbell <@t> fmh.org Tue Jul 1 10:19:41 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Tue Jul 1 10:19:40 2014 Subject: [Histonet] silver nitrate Message-ID: <3566D9E34287BE4B95372179009446A01F499CE7@EXCHANGE.fmhnt.fmh.org> So does anyone out there have a secret formula to get silver stain off counter tops????? Thanks for you the help!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From suetp918 <@t> comcast.net Tue Jul 1 10:23:17 2014 From: suetp918 <@t> comcast.net (Sue) Date: Tue Jul 1 10:23:35 2014 Subject: [Histonet] silver nitrate In-Reply-To: <3566D9E34287BE4B95372179009446A01F499CE7@EXCHANGE.fmhnt.fmh.org> Message-ID: <538091669.523064.1404228197290.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> You can rub salt on it and it should come up, I our salt on counter and make a thick scrub You can use Iodine and than wash with Sodum Thiosulfate Both work on hands the second one is a little more difficult to work with but does work From tmcampbell <@t> fmh.org Tue Jul 1 10:24:10 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Tue Jul 1 10:24:10 2014 Subject: [Histonet] silver nitrate In-Reply-To: <538091669.523064.1404228197290.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> References: <3566D9E34287BE4B95372179009446A01F499CE7@EXCHANGE.fmhnt.fmh.org> <538091669.523064.1404228197290.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: <3566D9E34287BE4B95372179009446A01F499CFE@EXCHANGE.fmhnt.fmh.org> Ill give it a try! Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) ________________________________ From: Sue [mailto:suetp918@comcast.net] Sent: Tuesday, July 01, 2014 11:23 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] silver nitrate You can rub salt on it and it should come up, I our salt on counter and make a thick scrub You can use Iodine and than wash with Sodum Thiosulfate Both work on hands the second one is a little more difficult to work with but does work From Lori.Disher <@t> HCAhealthcare.com Tue Jul 1 10:25:21 2014 From: Lori.Disher <@t> HCAhealthcare.com (Lori.Disher@HCAhealthcare.com) Date: Tue Jul 1 10:25:30 2014 Subject: [Histonet] Ideas for a Retiring Pathologist Message-ID: <778DD853CF606049A37FC2059C8BA07A8C7BF5C5AD@FWDCWPMSGCMS04.hca.corpad.net> I'm looking for creative ideas to celebrate a retiring pathologist sometime this year. What have you all done in the past? Lori Disher From jqb7 <@t> cdc.gov Tue Jul 1 10:30:10 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Jul 1 10:30:28 2014 Subject: [Histonet] RE: Ideas for a Retiring Pathologist In-Reply-To: <778DD853CF606049A37FC2059C8BA07A8C7BF5C5AD@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A8C7BF5C5AD@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: <3B2CD438E1628A41BD687E98B963B7811FDBF133@EMBX-CHAM2.cdc.gov> Depends on the pathologist. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori.Disher@HCAhealthcare.com Sent: Tuesday, July 01, 2014 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ideas for a Retiring Pathologist I'm looking for creative ideas to celebrate a retiring pathologist sometime this year. What have you all done in the past? Lori Disher _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jul 1 10:45:23 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 1 10:45:27 2014 Subject: [Histonet] dissecting microscope In-Reply-To: <25A4DE08332B19499904459F00AAACB719DE6DEECE@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719DE6DEECE@EVS1.archildrens.org> Message-ID: <1404229523.24712.YahooMailNeo@web120405.mail.ne1.yahoo.com> Go to eBay and?bid or buy the one you like. There are many options. Ren? J.? On Tuesday, July 1, 2014 11:14 AM, "Horn, Hazel V" wrote: We are looking for a dissecting microscope.? Nothing real fancy and it does not have to be new. Thanks.? Please email me and I will call you. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Jul 1 10:47:58 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jul 1 10:48:09 2014 Subject: [Histonet] RE: Ideas for a Retiring Pathologist In-Reply-To: <778DD853CF606049A37FC2059C8BA07A8C7BF5C5AD@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A8C7BF5C5AD@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: Create a photo album, including drawings, of all the folks he's worked with and all the funny things that have happened through the years - and make fun of him/her unmercifully... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori.Disher@HCAhealthcare.com Sent: Tuesday, July 01, 2014 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ideas for a Retiring Pathologist I'm looking for creative ideas to celebrate a retiring pathologist sometime this year. What have you all done in the past? Lori Disher _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From gu.lang <@t> gmx.at Tue Jul 1 12:18:38 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jul 1 12:18:59 2014 Subject: AW: [Histonet] Ideas for a Retiring Pathologist In-Reply-To: <778DD853CF606049A37FC2059C8BA07A8C7BF5C5AD@FWDCWPMSGCMS04.hca.corpad.net> References: <778DD853CF606049A37FC2059C8BA07A8C7BF5C5AD@FWDCWPMSGCMS04.hca.corpad.net> Message-ID: <000301cf9550$8055e970$8101bc50$@gmx.at> Buy him a ticket to Austria and let him work here with us. ; ) ..... still looking for pathologists.... Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Lori.Disher@HCAhealthcare.com Gesendet: Dienstag, 01. Juli 2014 17:25 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ideas for a Retiring Pathologist I'm looking for creative ideas to celebrate a retiring pathologist sometime this year. What have you all done in the past? Lori Disher _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Tue Jul 1 14:02:58 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Jul 1 14:03:07 2014 Subject: [Histonet] Re: Gram stain Message-ID: Tanya G. Abbott RT (CSMLS), Manager Technologist, Histology/Cytology, St. Joseph Medical Center, Reading PA asks: >>We only perform a few Gram stains a year, hardly seems worth It to keep a kit and expire reagents! Does anyone have an idea for other options? Ie, doing the stains in Micro? Checking with their BD rep to see if we can do their Gram stain kit on formalin fixed tissue.<< No, you cannot do a bacteriological type Gram stain on formalin fixed paraffin embedded tissue sections. What's needed here is some pathologist education. You're not trying to identify bacteria, you're trying to find them and maybe count them. For that you need a very simple blue stain such as Diff-Quik II or "Giemsa" or toluidine blue. Tissue Gram stains are not worth doing, and should be abandoned. Bob Richmond Samurai Pathologist Maryville TN From Maxim_71 <@t> mail.ru Tue Jul 1 14:18:29 2014 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Tue Jul 1 14:18:39 2014 Subject: [Histonet] silver nitrate Message-ID: <1167178141.20140701231829@mail.ru> Tasha, 1. Try 10% Potassium Iodide (KJ) for 5-10 minutes, spot will turn to yellow as lemon, then apply 10% Sodum Thiosulfate, wipe it and then talc powder and dry. 2. 15% sodium chloride with some drops hydrochloric acid, then apply 10% Sodum Thiosulfate, wash and dry. Maxim Peshkov, Russia, Taganrog. You wrote: So does anyone out there have a secret formula to get silver stain off counter tops????? Thanks for you the help!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) mailto:Maxim_71@mail.ru From s.murdoch <@t> dermpathnw.com Tue Jul 1 17:41:29 2014 From: s.murdoch <@t> dermpathnw.com (Stacey Murdoch) Date: Tue Jul 1 17:49:26 2014 Subject: [Histonet] Job opening - Bellevue, WA Message-ID: <53b33af0.856c440a.62f8.fffff896@mx.google.com> We are a small, busy dermatopathology lab in Bellevue, WA (Seattle Metro) looking for full time or part time histologist. As a full time employee, we provide a competitive salary and excellent benefits including medical and dental insurance, paid holidays, PTO, profit sharing, and 401K options. REQUIREMENTS * ASCP certification (HT/HTL) preferred, or registry eligible * 2 years laboratory experience preferred * Ability to cut and embed small biopsy specimens efficiently * Good interpersonal skills * Self direction in a fast paced environment * Ability to work Tuesday-Saturday 4am - 12 pm with occasional overtime Interested parties may contact me directly for additional information at s.murdoch@dermpathnw.com Thanks, Stacey From Susan.Walzer <@t> HCAHealthcare.com Wed Jul 2 02:10:06 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Wed Jul 2 02:10:13 2014 Subject: [Histonet] RE: Ideas for a Retiring Pathologist In-Reply-To: <3B2CD438E1628A41BD687E98B963B7811FDBF133@EMBX-CHAM2.cdc.gov> References: <778DD853CF606049A37FC2059C8BA07A8C7BF5C5AD@FWDCWPMSGCMS04.hca.corpad.net> <3B2CD438E1628A41BD687E98B963B7811FDBF133@EMBX-CHAM2.cdc.gov> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FBD46798C@FWDCWPMSGCMS09.hca.corpad.net> Over the years we saved interesting gallstones, all shapes and sizes...when it came time for the head Doc to retire we glued them on a black background board in an interesting swirl.. had it framed on a really nice shadowbox.. It looked beautiful.. Weird but beautiful! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, July 01, 2014 11:30 AM To: Disher Lori; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ideas for a Retiring Pathologist Depends on the pathologist. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori.Disher@HCAhealthcare.com Sent: Tuesday, July 01, 2014 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ideas for a Retiring Pathologist I'm looking for creative ideas to celebrate a retiring pathologist sometime this year. What have you all done in the past? Lori Disher _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson1 <@t> nhs.net Wed Jul 2 04:53:30 2014 From: kemlo.rogerson1 <@t> nhs.net (Rogerson Kemlo (THE WALTON CENTRE NHS FOUNDATION TRUST - RET)) Date: Wed Jul 2 04:53:39 2014 Subject: [Histonet] Best fixative for whole brains Message-ID: <20140702095331.BD4FB449216@nhs-pd1e-esg102.ad1.nhs.net> Dear All, Which is the best fixative for whole brains? Obviously must be able to carry out tinctural stains and immunoperoxidase procedures. Kemlo Rogerson Chartered Scientist Liverpool UK ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** From christina.kreutzer01 <@t> gmail.com Wed Jul 2 06:35:35 2014 From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer) Date: Wed Jul 2 06:35:39 2014 Subject: [Histonet] PET Membrane Slides Message-ID: Hello histonetters, does anybody of you have experience with PET Membrane Slices (Leica) for laser capture-dissection and how to coat them so that your slices won't swim off the slide? There is a Master Student in my group who would highly appreciate any help! Greetings Christina From fpearsa <@t> clemson.edu Wed Jul 2 07:14:12 2014 From: fpearsa <@t> clemson.edu (Fran Pearsall) Date: Wed Jul 2 07:14:21 2014 Subject: [Histonet] Refrigerator Temps Message-ID: <53B3F794.2040103@clemson.edu> I am wanting to know what the acceptable range is for a refrigerator which has Histo special stains and kits kept in it? We have gone from a mercury to a digital thermometer. Any input would be appreciated. Thanks. From wbenton <@t> cua.md Wed Jul 2 07:35:56 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Jul 2 07:37:43 2014 Subject: [Histonet] Refrigerator Temps In-Reply-To: <53B3F794.2040103@clemson.edu> References: <53B3F794.2040103@clemson.edu> Message-ID: <0B8979A204680A42B93A52B486088CD93938636905@CUAEXH1.GCU-MD.local> Most reagents have an acceptable range on the label that goes from 2-8 degrees Celsius provided they have been commercially prepared. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fran Pearsall [fpearsa@clemson.edu] Sent: Wednesday, July 02, 2014 8:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerator Temps I am wanting to know what the acceptable range is for a refrigerator which has Histo special stains and kits kept in it? We have gone from a mercury to a digital thermometer. Any input would be appreciated. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Lyndsey.Buechel <@t> covance.com Wed Jul 2 09:22:40 2014 From: Lyndsey.Buechel <@t> covance.com (Buechel, Lyndsey) Date: Wed Jul 2 09:22:49 2014 Subject: [Histonet] Job Opportunities with Covance! Message-ID: Hi everyone, Due to growth, Covance has multiple Histology positions available in Madison, WI - we are offering a sign on bonus and relocation assistance to qualified candidates. Positions are available at different experience levels and offer the opportunity to be cross trained in necropsy. If you'd be interested in learning more you can view the full description here or reach out to me directly: http://jobsearch.covance.com/jobs/1008004-Research-Assistant-Histotechnnician-Pathology-Madison-WI.aspx We offer: - Competitive wages - Full benefit package starting on your first day - 21 days of paid time off - Advancement opportunities and so much more! Happy Wednesday! Lyndsey Buechel Recruiter Recruitment and Talent Services Covance Inc. Minneapolis, MN Tel: +1 218 297 4125 E-mail: lyndsey.buechel@covance.com Apply online at: www.covancecareers.com ________________________________ Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From cbrya <@t> lexclin.com Wed Jul 2 10:36:20 2014 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Wed Jul 2 10:36:24 2014 Subject: [Histonet] cases grossed per day Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF4C0B7BE6A0@EXCHANGESB> Hello all, Does anyone have guidelines on how many cases and specimens a good, competent Pathologist Assistant should be able to gross per 8 hour day? Thanks for any input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From rjbuesa <@t> yahoo.com Wed Jul 2 10:53:11 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 2 10:53:17 2014 Subject: [Histonet] cases grossed per day In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF4C0B7BE6A0@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF4C0B7BE6A0@EXCHANGESB> Message-ID: <1404316391.2473.YahooMailNeo@web120404.mail.ne1.yahoo.com> The average is 48 cases/8 hours (? 6 cases/hour ? 10 min/case) For additional information log on ?http://www.histosearch.com/rene/html?and read the article on histology benchmarks Ren? J. On Wednesday, July 2, 2014 11:36 AM, Carol Bryant wrote: Hello all, Does anyone have guidelines on how many cases and specimens a good, competent Pathologist Assistant should be able to gross per 8 hour day? Thanks for any input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations.? If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited.? Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Wed Jul 2 11:07:06 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Wed Jul 2 11:04:19 2014 Subject: [Histonet] cases grossed per day In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF4C0B7BE6A0@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF4C0B7BE6A0@EXCHANGESB> Message-ID: <001c01cf960f$acafec40$060fc4c0$@caplab.org> That depends on what type of lab you are. Hospital setting, Derm Lab, GI Lab, etc. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Wednesday, July 02, 2014 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cases grossed per day Hello all, Does anyone have guidelines on how many cases and specimens a good, competent Pathologist Assistant should be able to gross per 8 hour day? Thanks for any input. Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4592 / Virus Database: 3986/7778 - Release Date: 07/01/14 From JCBRITTON <@t> Cheshire-Med.COM Wed Jul 2 11:17:59 2014 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Wed Jul 2 11:23:06 2014 Subject: [Histonet] RE: Ideas for a Retiring Pathologist In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FBD46798C@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: For our retiring Pathologist, we typed up a results report with his name on it. Everyone in the department got to make a statement in it about him. It was quite humorous and he got a real kick out of it! Josie Britton -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan.Walzer@HCAHealthcare.com Sent: Wednesday, July 02, 2014 3:10 AM To: jqb7@cdc.gov; Lori.Disher@HCAhealthcare.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ideas for a Retiring Pathologist Over the years we saved interesting gallstones, all shapes and sizes...when it came time for the head Doc to retire we glued them on a black background board in an interesting swirl.. had it framed on a really nice shadowbox.. It looked beautiful.. Weird but beautiful! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, July 01, 2014 11:30 AM To: Disher Lori; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ideas for a Retiring Pathologist Depends on the pathologist. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori.Disher@HCAhealthcare.com Sent: Tuesday, July 01, 2014 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ideas for a Retiring Pathologist I'm looking for creative ideas to celebrate a retiring pathologist sometime this year. What have you all done in the past? Lori Disher _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Michael.Baker <@t> cchmc.org Wed Jul 2 12:27:28 2014 From: Michael.Baker <@t> cchmc.org (Baker, Michael) Date: Wed Jul 2 12:27:38 2014 Subject: [Histonet] Re: Gram stain In-Reply-To: <80.C2.05540.32E34B35@mx8.cchmc.org> References: <80.C2.05540.32E34B35@mx8.cchmc.org> Message-ID: <0556BFF5-37EA-49F7-9AA6-049D4C8F681D@cchmc.org> Seconding Dr. Richmond's advice; a Wright-Giemsa stain is excellent for highlighting bacteria. ---- Michael Baker, M.D. CCHMC Pathology Pager 736-1404 From barbara.tibbs <@t> accuratediagnosticlabs.com Wed Jul 2 13:46:22 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Wed Jul 2 13:46:30 2014 Subject: [Histonet] RE: Best fixative for whole brains In-Reply-To: <20140702095331.BD4FB449216@nhs-pd1e-esg102.ad1.nhs.net> References: <20140702095331.BD4FB449216@nhs-pd1e-esg102.ad1.nhs.net> Message-ID: <1404326772245.34135@accuratediagnosticlabs.com> What species of brain are we talking about? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rogerson Kemlo (THE WALTON CENTRE NHS FOUNDATION TRUST - RET) Sent: Wednesday, July 02, 2014 8:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best fixative for whole brains Dear All, Which is the best fixative for whole brains? Obviously must be able to carry out tinctural stains and immunoperoxidase procedures. Kemlo Rogerson Chartered Scientist Liverpool UK ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Arzt <@t> ARS.USDA.GOV Wed Jul 2 20:31:48 2014 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Wed Jul 2 20:31:59 2014 Subject: [Histonet] Veterinary Histotechnologist Job at Plum Island USDA Message-ID: <7213AC15544DE945843D821E1769E38A0680A30C@001FSN2MPN1-052.001f.mgd2.msft.net> [CLOSING FOR APPLICATION JULY 8 2014] A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old Saybrook, CT. Workload is centered on veterinary viral pathogenesis research; emphasis is foot-and-mouth disease and African swine fever. Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400 Salary $44,175.00 to $84,990.00 / per year commensurate with experience. Federal benefits package. ******************************************************** Jonathan Arzt DVM, MPVM, PhD, DACVP Veterinary Medical Officer (Pathology) Foreign Animal Disease Research Unit USDA/ARS Plum Island Animal Disease Center P.O. Box 848, Greenport NY 11944 631-323-3336(ph) 631-323-3006(fax) Jonathan.Arzt@ARS.USDA.GOV ******************************************************** This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. From traczyk7 <@t> aol.com Fri Jul 4 04:03:04 2014 From: traczyk7 <@t> aol.com (traczyk7@aol.com) Date: Fri Jul 4 04:03:31 2014 Subject: [Histonet] Her2Neu by PCR Message-ID: <8D1657DE66750C5-23C4-1AC@webmail-m275.sysops.aol.com> Looking for a reference lab that does Her2 Neu by PCR. Our regular go to places don't offer it. Any suggestions would be appreciated. Thanks, Dorothy Dorothy Traczyk BS, HT (ASCP) Histology Laboratory Monmouth Medical Center Long Branch, NJ From rmire <@t> cvpath.org Mon Jul 7 08:56:17 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Mon Jul 7 08:56:25 2014 Subject: [Histonet] Thermo Shandon Excelsior tissue processor Message-ID: <2A1E6E7A-0CE2-4A6D-BB69-743DC639EC38@cvpath.org> Hello Netters, I need some suggestions. I recently started a new job in a research facility. They do mostly cardiovascular work and work with stents and valves. I came from clinical and all paraffin. The research lab does paraffin and plastic . Before I started, they attempted to convert a processor that had been used for paraffin processing to a plastic processor. Not much difference in processing, still uses same reagents but of course, no paraffin. The problem is this - no one here seems to know this processor other than pressing start, i have tried unsuccessfully to reprogram the instrument, I have transferred data from the other plastic processor, which is the same model but has always been used for plastics. I can process but I constantly have error codes and alarms. I have never worked with this model of processor before. The suggestion from one of the other techs has been to purchase a new processor. That may be necessary but they paid over $1000 for a company to clean and "convert" this processor and I think that I should try to get it to work instead of just buying new. Is there anyone out there who can help me with this. We do not have a service contract with Thermo. I am hoping someone works on this type of instrument and can help me get it running properly. Best, Ronda Mire CVPath Institute Gaithersburg, MD From algranth <@t> email.arizona.edu Mon Jul 7 09:59:01 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Jul 7 09:59:15 2014 Subject: [Histonet] Thermo Shandon Excelsior tissue processor In-Reply-To: <2A1E6E7A-0CE2-4A6D-BB69-743DC639EC38@cvpath.org> References: <2A1E6E7A-0CE2-4A6D-BB69-743DC639EC38@cvpath.org> Message-ID: <91E3A4E9-4AF6-4EA2-A7C4-91FBD2BFD687@email.arizona.edu> Probably wouldn't be difficult if we knew what processor it was. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From rmire <@t> cvpath.org Mon Jul 7 10:17:44 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Mon Jul 7 10:17:53 2014 Subject: [Histonet] Thermo Shandon Excelsior tissue processor In-Reply-To: <91E3A4E9-4AF6-4EA2-A7C4-91FBD2BFD687@email.arizona.edu> References: <2A1E6E7A-0CE2-4A6D-BB69-743DC639EC38@cvpath.org> <91E3A4E9-4AF6-4EA2-A7C4-91FBD2BFD687@email.arizona.edu> Message-ID: <800E4AEB-AC50-452F-887D-467EED02ADE7@cvpath.org> The processor is a Thermo Shandon Excelsior On Jul 7, 2014, at 10:59 AM, "Grantham, Andrea L - (algranth)" wrote: > Probably wouldn't be difficult if we knew what processor it was. > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > From Betty.Gast <@t> hshs.org Mon Jul 7 13:52:06 2014 From: Betty.Gast <@t> hshs.org (Gast, Betty L) Date: Mon Jul 7 13:52:14 2014 Subject: [Histonet] protocol for c-myc VMS platform. Message-ID: <468705ED810A34468A8650CFC201A800B45FD23E@EWJMB001.EW.HSHSAD.ORG> Would anyone share their protocols for c-myc/myc using the Ventana platform? Both IHC and ISH. Thank you! ~ ~ ~ ~ Betty Gast HT (ASCP) St. Mary's Hospital Medical Center 1726 Shawano Ave Green Bay, WI 54303 Phone: 920.884.4777 Fax: 920.884.3566 Betty.Gast@hshs.org Respect, Care, Competence, Joy LEGAL DISCLAIMER: This message and all attachments may be confidential or protected by privilege. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the information contained in or attached to this message is strictly prohibited. Please notify the sender of the delivery error by replying to this message, and then delete it from your system. Thank you. From ahorvath <@t> cogipath.com Mon Jul 7 14:17:09 2014 From: ahorvath <@t> cogipath.com (Andrew Horvath) Date: Mon Jul 7 14:16:29 2014 Subject: [Histonet] Thermo Shandon Excelsior tissue processor In-Reply-To: <800E4AEB-AC50-452F-887D-467EED02ADE7@cvpath.org> References: <2A1E6E7A-0CE2-4A6D-BB69-743DC639EC38@cvpath.org> <91E3A4E9-4AF6-4EA2-A7C4-91FBD2BFD687@email.arizona.edu> <800E4AEB-AC50-452F-887D-467EED02ADE7@cvpath.org> Message-ID: <005501cf9a18$0c3de7f0$24b9b7d0$@cogipath.com> We have the same type of processor (using paraffin) but I listed the person's information who provides service to ours and perhaps he can help. He worked at Thermo a handful of years specializing on processors so he knows them very well. Also, he does an excellent job providing PMs and general support. Chris Morris Jester Biomedical Services, LLC Phone: (269) 365-5960 Andrew Horvath, MBA, MA Operations Manager 303-770-4848 (O) 303-770-6641 (fax) ? Colorado GI Pathology 7346 S. Alton Way Suite 10E Centennial, CO 80112 Confidentiality Notice: This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, or confidential.? If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited.? If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire Sent: Monday, July 07, 2014 9:18 AM To: Grantham, Andrea L - (algranth) Cc: Subject: Re: [Histonet] Thermo Shandon Excelsior tissue processor The processor is a Thermo Shandon Excelsior On Jul 7, 2014, at 10:59 AM, "Grantham, Andrea L - (algranth)" wrote: > Probably wouldn't be difficult if we knew what processor it was. > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson1 <@t> nhs.net Tue Jul 8 02:49:24 2014 From: kemlo.rogerson1 <@t> nhs.net (Rogerson Kemlo (THE WALTON CENTRE NHS FOUNDATION TRUST - RET)) Date: Tue Jul 8 02:54:28 2014 Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 5 In-Reply-To: <20140707170312.74E70449F46@nhs-pd1e-esg004.ad1.nhs.net> References: <20140707170312.74E70449F46@nhs-pd1e-esg004.ad1.nhs.net> Message-ID: <20140708075349.919FA4490DB@nhs-pd1e-esg106.ad1.nhs.net> Dear Ronda Obviously you've tried the company that cleaned and converted the machine for assistance? I think the issue you may have is that Thermo may not want to get involved with a machine converted by a third party (you can understand why) and I'd have thought that the third party retained some responsibilities too. Regards Kemlo Rogerson Pathology Systems Manager The Walton Centre Liverpool ---------------------------------------------------------------------- Message: 1 Date: Mon, 7 Jul 2014 09:56:17 -0400 From: Ronda Mire Subject: [Histonet] Thermo Shandon Excelsior tissue processor To: histonet@lists.utsouthwestern.edu Message-ID: <2A1E6E7A-0CE2-4A6D-BB69-743DC639EC38@cvpath.org> Content-Type: text/plain; charset=us-ascii Hello Netters, I need some suggestions. I recently started a new job in a research facility. They do mostly cardiovascular work and work with stents and valves. I came from clinical and all paraffin. The research lab does paraffin and plastic . Before I started, they attempted to convert a processor that had been used for paraffin processing to a plastic processor. Not much difference in processing, still uses same reagents but of course, no paraffin. The problem is this - no one here seems to know this processor other than pressing start, i have tried unsuccessfully to reprogram the instrument, I have transferred data from the other plastic processor, which is the same model but has always been used for plastics. I can process but I constantly have error codes and alarms. I have never worked with this model of processor before. The suggestion from one of the other techs has been to purchase a new processor. That may be necessary but they paid over $1000 for a company to clean and " convert" this processor and I think that I should try to get it to work instead of just buying new. Is there anyone out there who can help me with this. We do not have a service contract with Thermo. I am hoping someone works on this type of instrument and can help me get it running properly. Best, Ronda Mire CVPath Institute Gaithersburg, MD ------------------------------ Message: 2 Date: Mon, 7 Jul 2014 14:59:01 +0000 From: "Grantham, Andrea L - (algranth)" Subject: Re: [Histonet] Thermo Shandon Excelsior tissue processor To: Ronda Mire Cc: "" Message-ID: <91E3A4E9-4AF6-4EA2-A7C4-91FBD2BFD687@email.arizona.edu> Content-Type: text/plain; charset="us-ascii" Probably wouldn't be difficult if we knew what processor it was. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ------------------------------ Message: 3 Date: Mon, 7 Jul 2014 11:17:44 -0400 From: Ronda Mire Subject: Re: [Histonet] Thermo Shandon Excelsior tissue processor To: "Grantham, Andrea L - (algranth)" Cc: "" Message-ID: <800E4AEB-AC50-452F-887D-467EED02ADE7@cvpath.org> Content-Type: text/plain; charset=us-ascii The processor is a Thermo Shandon Excelsior On Jul 7, 2014, at 10:59 AM, "Grantham, Andrea L - (algranth)" wrote: > Probably wouldn't be difficult if we knew what processor it was. > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 128, Issue 5 **************************************** ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** From massimo.tosi_m <@t> libero.it Tue Jul 8 02:44:19 2014 From: massimo.tosi_m <@t> libero.it (massimo.tosi_m) Date: Tue Jul 8 03:08:25 2014 Subject: [Histonet] massimo.tosi_m@libero.it Message-ID: <79AB56D903ABC2CB4952A82B9761814B@bondiphotos.com.au> http://kresypiekneznatury.pl/uiiy/zudzmrrk.dwfvkrlkqlqq From Jonathan.Arzt <@t> ARS.USDA.GOV Tue Jul 8 08:10:20 2014 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Tue Jul 8 08:11:25 2014 Subject: [Histonet] Last Day: Veterinary Histotechnologist Job at Plum Island USDA Message-ID: <7213AC15544DE945843D821E1769E38A0680AA76@001FSN2MPN1-052.001f.mgd2.msft.net> [CLOSING FOR APPLICATION JULY 8 2014] A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old Saybrook, CT. Workload is centered on veterinary viral pathogenesis research; emphasis is foot-and-mouth disease and African swine fever. Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400 Salary $44,175.00 to $84,990.00 / per year commensurate with experience. Federal benefits package. This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. From smah2 <@t> msn.com Tue Jul 8 11:45:16 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Tue Jul 8 11:45:21 2014 Subject: [Histonet] Pathology Transcription Message-ID: I have a couple of questions... One, what do you all use for resources for finding a good pathology transcriptionist? We've done the typical things but have been unable to fill our open position with a strong candidate. Second, I would like to cut down on the amount of paper shuffling our transcription department does. It's unbelievable! The transcriptionist handles the paperwork about 4-5 times before it is stacked and prepared by another area for scanning. Any suggestions? We cannot go completely paperless at this time due to the upfront expense but I am interested in trimming down the handling that is done in transcription so the transcriptionist can focus more on typing rather than hunting requisitions. Part of the issue is the pathologists desire to hold each requisition in their hands so I am fighting that battle as well. Thank you in advance for your input! I'd appreciate any suggestions. Sheila Haas MicroPath Laboratories, Inc. From christina.wolfe <@t> bms.com Tue Jul 8 11:58:15 2014 From: christina.wolfe <@t> bms.com (Wolfe, Christina) Date: Tue Jul 8 11:58:23 2014 Subject: [Histonet] Help Please - Need a Leica Rep to contact me Message-ID: I am in Indiana - I need a Leica sales rep to contact me please or if someone can provide me with a regional sales rep name - that would be awesome! Thanks! Kristie Ph 812-307-2093 christina.wolfe@bms.com ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From rjbuesa <@t> yahoo.com Tue Jul 8 12:05:37 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 8 12:05:46 2014 Subject: [Histonet] Pathology Transcription In-Reply-To: References: Message-ID: <1404839137.24971.YahooMailNeo@web120405.mail.ne1.yahoo.com> Yours is the typical situation all pathology departments confronted way back in the 1970's before starting to use computers. To start I think you should do a cost study of how much you are spending in all this paperwork and compare it to the "up-front" cost to go paperless. Perhaps this comparison will convince your pathologists that it is worth coming out of the "ancient world" to the present.Meanwhile you should study all the forms you use and delete those not completely "vital" for?record keeping. As to your transcriptionist I advise?you to contact a training facility, ask for the "best trainee" and try it. Perhaps you will get a "diamond in brute" you can "cut to perfection". Ren? J.? On Tuesday, July 8, 2014 12:45 PM, Sheila Haas wrote: I have a couple of questions... One, what do you all use for resources for finding a good pathology transcriptionist? We've done the typical things but have been unable to fill our open position with a strong candidate. Second, I would like to cut down on the amount of paper shuffling our transcription department does. It's unbelievable! The transcriptionist handles the paperwork about 4-5 times before it is stacked and prepared by another area for scanning. Any suggestions? We cannot go completely paperless at this time due to the upfront expense but I am interested in trimming down the handling that is done in transcription so the transcriptionist can focus more on typing rather than hunting requisitions. Part of the issue is the pathologists desire to hold each requisition in their hands so I am fighting that battle as well. Thank you in advance for your input! I'd appreciate any suggestions. Sheila Haas MicroPath Laboratories, Inc. ??? ??? ??? ? ??? ??? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NIngrao <@t> KaleidaHealth.Org Tue Jul 8 12:23:19 2014 From: NIngrao <@t> KaleidaHealth.Org (Ingrao, Nicholas) Date: Tue Jul 8 12:23:26 2014 Subject: [Histonet] Sekura Tissue Tek Block Storage Message-ID: Hello Histonet, First time poster. My institution has a 25 year retention on paraffin blocks. The 1989 batch was just destroyed and the storage containers were returned to me. They appear to be the same size as the current sekura tissue block storage units we use. Does anyone know of a commercially available label overlay for the drawers? I could cut down adhesive labels, but wanted to see what was out there on the market. Thanks, Nick Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health’s Technology Assistance Center at (716) 859-7777. From smah2 <@t> msn.com Tue Jul 8 12:26:58 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Tue Jul 8 12:27:02 2014 Subject: [Histonet] Sekura Tissue Tek Block Storage In-Reply-To: References: Message-ID: As we reuse block file drawers, we run a piece of bold colored tape (as wide as the front of the drawer) across the front. We use a different color for each year which helps quickly distinguish one year from the next when pulling or filing blocks. Works very well here and is inexpensive. Good luck. Sheila Haas MicroPath Laboratories, Inc. > From: NIngrao@KaleidaHealth.Org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 8 Jul 2014 17:23:19 +0000 > Subject: [Histonet] Sekura Tissue Tek Block Storage > > Hello Histonet, > > First time poster. My institution has a 25 year retention on paraffin blocks. The 1989 batch was just destroyed and the storage containers were returned to me. They appear to be the same size as the current sekura tissue block storage units we use. Does anyone know of a commercially available label overlay for the drawers? I could cut down adhesive labels, but wanted to see what was out there on the market. > > Thanks, Nick > > > Nick Ingrao, MBA, MT(ASCP) > Manager, Anatomic Pathology > Kaleida Health: Buffalo General Medical Center > 100 High St > Buffalo, NY 14203 > > Office:(716) 859-2028 > Pager: (716) 642-0232 > Fax: (716) 859-2393 > > > > The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health??s Technology Assistance Center at (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Tue Jul 8 12:38:19 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Jul 8 12:38:29 2014 Subject: [Histonet] Sekura Tissue Tek Block Storage In-Reply-To: References: Message-ID: We re-use block file drawers as well. We write on the front with red sharpie marker and take off writing with alcohol. Works pretty well. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 7/8/14, 11:26 AM, "Sheila Haas" wrote: >As we reuse block file drawers, we run a piece of bold colored tape (as >wide as the front of the drawer) across >the front. We use a different color for each year which helps quickly >distinguish one year from the next when pulling or filing blocks. Works >very well here and is inexpensive. > >Good luck. >Sheila Haas >MicroPath Laboratories, Inc. > > > >> From: NIngrao@KaleidaHealth.Org >> To: histonet@lists.utsouthwestern.edu >> Date: Tue, 8 Jul 2014 17:23:19 +0000 >> Subject: [Histonet] Sekura Tissue Tek Block Storage >> >> Hello Histonet, >> >> First time poster. My institution has a 25 year retention on paraffin >>blocks. The 1989 batch was just destroyed and the storage containers >>were returned to me. They appear to be the same size as the current >>sekura tissue block storage units we use. Does anyone know of a >>commercially available label overlay for the drawers? I could cut down >>adhesive labels, but wanted to see what was out there on the market. >> >> Thanks, Nick >> >> >> Nick Ingrao, MBA, MT(ASCP) >> Manager, Anatomic Pathology >> Kaleida Health: Buffalo General Medical Center >> 100 High St >> Buffalo, NY 14203 >> >> Office:(716) 859-2028 >> Pager: (716) 642-0232 >> Fax: (716) 859-2393 >> >> >> >> The Keeping You Informed section of Kaleida Health`s website features a >>wealth of information, stories and pictures about our valued workforce >>and the tremendous momentum our organization is experiencing. Check us >>out at: www.kaleidahealth.org/kyi >> >> CONFIDENTIALITY NOTICE: This email transmission and any documents, >>files, or previous e-mail messages attached to it are confidential and >>intended solely for the use of the individual or entity to whom they are >>addressed. If you are not the intended recipient, or a person >>responsible for delivering it to the intended recipient, you are hereby >>notified that any further review, disclosure, copying, dissemination, >>distribution, or use of any of the information contained in or attached >>to this e-mail transmission is strictly prohibited. If you have received >>this message in error, please notify the sender immediately by e-mail, >>discard any paper copies, and delete all electronic files of the >>message. If you are unable to contact the sender or you are not sure as >>to whether you are the intended recipient, please call Kaleida Health??s >>Technology Assistance Center at (716) 859-7777. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lesley.Bechtold <@t> jax.org Tue Jul 8 12:44:06 2014 From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold) Date: Tue Jul 8 12:44:10 2014 Subject: [Histonet] Prioritizing Histology work/projects Message-ID: <08997064E2075247A4DD5A035DB51CDF64FA5B9B@jaxbhexms02.jax.org> Good Afternoon, This may apply more to the research-type labs than clinical labs, but I am wondering: how do you prioritize the work that your Histology Lab or Service receives? Do you batch like things together? Do it strictly by date? Are there other ways to do it? I'd be interested in how it's done in other places. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From christina.wolfe <@t> bms.com Tue Jul 8 12:49:39 2014 From: christina.wolfe <@t> bms.com (Wolfe, Christina) Date: Tue Jul 8 12:49:45 2014 Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 6 - Leica Rep Question In-Reply-To: References: Message-ID: Hello all, Thanks for the prompt response to my inquiry to find an equipment sales rep! Leica has very quickly been attentive to help me out. Great job Mike & Dave - thanks again! Kristie Message: 7 Date: Tue, 8 Jul 2014 12:58:15 -0400 From: "Wolfe, Christina" Subject: [Histonet] Help Please - Need a Leica Rep to contact me I am in Indiana - I need a Leica sales rep to contact me please or if someone can provide me with a regional sales rep name - that would be awesome! Thanks! Kristie Ph 812-307-2093 christina.wolfe@bms.com ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 128, Issue 6 **************************************** This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From BSullivan <@t> virtua.org Tue Jul 8 13:02:46 2014 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Tue Jul 8 13:02:52 2014 Subject: [Histonet] pre-ordering special stains Message-ID: <6932520047F7EE46B512E9801344F16080026370@ExchangeMB-1.Virtua.org> I know this was discussed just recently but I need feedback on the current trending to not pre-order specials based on tissue type. Example: Antral bx receives a diff quick, silver stain or IHC stain before looking at the H and E stain. I think the last I remember reading on this site was that certain insurances were not paying if not needed. Thanking you in advance. Beatrice L. Sullivan Corporate Histology Supervisor Virtua, Voorhees 856-247-3144 Courage is what it takes to stand up and speak. Courage is also what it takes to sit down and listen. This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From rjr6 <@t> psu.edu Tue Jul 8 13:06:50 2014 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Tue Jul 8 13:06:56 2014 Subject: [Histonet] RE: Prioritizing Histology work/projects In-Reply-To: <08997064E2075247A4DD5A035DB51CDF64FA5B9B@jaxbhexms02.jax.org> References: <08997064E2075247A4DD5A035DB51CDF64FA5B9B@jaxbhexms02.jax.org> Message-ID: I work for Penn State's Animal Diagnostic lab and I get diagnostic samples and research samples. My priorities are diagnostic first then the research is done in the order it comes to the lab Roberta Horner Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Tuesday, July 08, 2014 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prioritizing Histology work/projects Good Afternoon, This may apply more to the research-type labs than clinical labs, but I am wondering: how do you prioritize the work that your Histology Lab or Service receives? Do you batch like things together? Do it strictly by date? Are there other ways to do it? I'd be interested in how it's done in other places. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jbro22 <@t> lsuhsc.edu Tue Jul 8 13:20:36 2014 From: jbro22 <@t> lsuhsc.edu (Browning, Jeffrey A.) Date: Tue Jul 8 13:20:47 2014 Subject: [Histonet] Autopsy, Histology, & Transcription positions in Shreveport, LA Message-ID: <2E3D5719BC42374E982EF0AD611F2362B8572FA9@SH-ExchMB1.master.lsuhsc.edu> The Department of Pathology at University Health Shreveport is currently advertising full-time positions for: histotechnologist; histology supervisor; autopsy diener ("AP Technician"); medical transcriptionist. Interested individuals may view the listings and apply at https://careers.uhsystem.com/application.aspx. Jeff Browning, HTL(ASCP) Technical Director, Anatomic Pathology Department of Pathology LSU Health Science Center - Shreveport 1501 Kings Highway Shreveport, LA 71103 (318) 675-5872 jbro22@lsuhsc.edu From rennie1108 <@t> yahoo.com Tue Jul 8 14:18:00 2014 From: rennie1108 <@t> yahoo.com (Adrienne Anderson) Date: Tue Jul 8 14:18:15 2014 Subject: [Histonet] HBV Control Block Message-ID: <5E02AF03-4E58-40E3-A984-705D8EA372AB@yahoo.com> Hello all, We are in need of a HepB control block. Does anyone have any recommendations for procuring this? I actually only need a few slides, so I?m just feeling things out. Any advice would be appreciated. Thanks! Best regards, Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. From Betty.Gast <@t> hshs.org Tue Jul 8 15:37:36 2014 From: Betty.Gast <@t> hshs.org (Gast, Betty L) Date: Tue Jul 8 15:37:49 2014 Subject: [Histonet] HTL wage range Message-ID: <468705ED810A34468A8650CFC201A800B45FD328@EWJMB001.EW.HSHSAD.ORG> Hi, I was wondering if anyone would share your salary range for a new HTL grad. I have the ASCP 2013 survey and wanted to compare. Thanks! Betty Gast HT(ASCP) betty.gast@hshs.org Histology Facilitator St. Mary's PAthology Department Green Bay, WI 54303 LEGAL DISCLAIMER: This message and all attachments may be confidential or protected by privilege. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the information contained in or attached to this message is strictly prohibited. Please notify the sender of the delivery error by replying to this message, and then delete it from your system. Thank you. From ehanson <@t> carisls.com Wed Jul 9 08:51:19 2014 From: ehanson <@t> carisls.com (Hanson, Eric) Date: Wed Jul 9 08:51:26 2014 Subject: [Histonet] Bulk Tissue Procurement Message-ID: <03D535A75AB84D44AFC75161BE5BE6CF032A26A42A@S-AZ-MLBX02.CLSNET.intranet> Good morning histonetters! We are looking for USA vendors for bulk tissue. Mostly we are short on HER2+ breast and cervical epidermal tissue (which is punchable, where skin is not). We make our own tissue microarray and also use gallbladder, prostate, uterus/myometrium, and kidney. Surely we are not the only lab that needs these tissue types, and I hope someone out there can point me in the direction of some new vendors. Thanks, Eric Hanson CMI Histotech I Caris Life Sciences 4610 South 44th Place Phoenix, AZ 85040 ehanson@carisls.com From rjbuesa <@t> yahoo.com Wed Jul 9 09:30:12 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 9 09:30:18 2014 Subject: [Histonet] Bulk Tissue Procurement In-Reply-To: <03D535A75AB84D44AFC75161BE5BE6CF032A26A42A@S-AZ-MLBX02.CLSNET.intranet> References: <03D535A75AB84D44AFC75161BE5BE6CF032A26A42A@S-AZ-MLBX02.CLSNET.intranet> Message-ID: <1404916212.21333.YahooMailNeo@web120405.mail.ne1.yahoo.com> Tissue vendors are very scarce and highly regulated to the point that such a "trade" is almost non-existent. I would advise you to get in contact with the Medical Examiner in your county and try to obtain tissue from them. Ren? J.? On Wednesday, July 9, 2014 9:51 AM, "Hanson, Eric" wrote: Good morning histonetters! We are looking for USA vendors for bulk tissue. Mostly we are short on HER2+ breast and cervical epidermal tissue (which is punchable, where skin is not). We make our own tissue microarray and also use gallbladder, prostate, uterus/myometrium, and kidney. Surely we are not the only lab that needs these tissue types, and I hope someone out there can point me in the direction of some new vendors. Thanks, Eric Hanson CMI Histotech I Caris Life Sciences 4610 South 44th Place Phoenix, AZ 85040 ehanson@carisls.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jul 9 09:35:48 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 9 09:35:52 2014 Subject: [Histonet] HTL wage range In-Reply-To: <468705ED810A34468A8650CFC201A800B45FD328@EWJMB001.EW.HSHSAD.ORG> References: <468705ED810A34468A8650CFC201A800B45FD328@EWJMB001.EW.HSHSAD.ORG> Message-ID: <1404916548.5205.YahooMailNeo@web120404.mail.ne1.yahoo.com> Salaries vary wildly and depend on the area you are working. Having said that my advise is always that you should decide first?how much would you need?for your living expenses and make that?amount?your bottom salary and then apply for a position and?try to obtain that amount REGARDLESS of what they are offering and the market place is. You have to be "aggressive" in your pursue of a salary that will allow you to live as you desire. Remember that working for an amount of money that will let you "just get by" is one of the most frustrating experiences there are. Having said that?under separate cover I am forwarding you an article I wrote on the subject. Ren? J.?? On Tuesday, July 8, 2014 4:38 PM, "Gast, Betty L" wrote: Hi, I was wondering if anyone would share your salary range for a new HTL grad. I have the ASCP 2013 survey and wanted to compare. Thanks! Betty Gast HT(ASCP) betty.gast@hshs.org Histology Facilitator St. Mary's PAthology Department Green Bay, WI 54303 LEGAL DISCLAIMER: This message and all attachments may be confidential or protected by privilege. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the information contained in or attached to this message is strictly prohibited. Please notify the sender of the delivery error by replying to this message, and then delete it from your system. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jul 9 09:47:38 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 9 09:47:44 2014 Subject: [Histonet] RE: Prioritizing Histology work/projects In-Reply-To: References: <08997064E2075247A4DD5A035DB51CDF64FA5B9B@jaxbhexms02.jax.org> Message-ID: <1404917258.12569.YahooMailNeo@web120405.mail.ne1.yahoo.com> Regardless of what you are going to do with the samples later on or who is going to use them?for, you should always batch the samples of any given date and process them. Ren? J.? On Tuesday, July 8, 2014 2:07 PM, Roberta Horner wrote: I work for Penn State's Animal Diagnostic lab and I get diagnostic samples and research samples.? My priorities are diagnostic first then the research is done in the order it comes to the lab Roberta Horner Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Tuesday, July 08, 2014 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prioritizing Histology work/projects Good Afternoon, This may apply more to the research-type labs than clinical labs, but I am wondering: how do you prioritize the work that your Histology Lab or Service receives?? Do you batch like things together? Do it strictly by date?? Are there other ways to do it? I'd be interested in how it's done in other places. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Wed Jul 9 09:53:37 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Jul 9 09:53:49 2014 Subject: [Histonet] RE: Bulk Tissue Procurement In-Reply-To: <03D535A75AB84D44AFC75161BE5BE6CF032A26A42A@S-AZ-MLBX02.CLSNET.intranet> References: <03D535A75AB84D44AFC75161BE5BE6CF032A26A42A@S-AZ-MLBX02.CLSNET.intranet> Message-ID: Eric - here a few to check out if you haven't already http://www.asterand.com/Asterand/ https://www.absbio.com/ http://www.capitalbiosciences.com/ http://www.chtn.nci.nih.gov/ http://ndriresource.org/Human-Tissue-Services/Online-Biospecimen-Catalogue/135/ http://www.tissue-solutions.com/prod_fresh.html http://www.amsbio.com/home.aspx Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hanson, Eric Sent: Wednesday, July 09, 2014 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bulk Tissue Procurement Good morning histonetters! We are looking for USA vendors for bulk tissue. Mostly we are short on HER2+ breast and cervical epidermal tissue (which is punchable, where skin is not). We make our own tissue microarray and also use gallbladder, prostate, uterus/myometrium, and kidney. Surely we are not the only lab that needs these tissue types, and I hope someone out there can point me in the direction of some new vendors. Thanks, Eric Hanson CMI Histotech I Caris Life Sciences 4610 South 44th Place Phoenix, AZ 85040 ehanson@carisls.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From bbrinegarhtl <@t> gmail.com Wed Jul 9 12:16:55 2014 From: bbrinegarhtl <@t> gmail.com (Beth Brinegar) Date: Wed Jul 9 12:17:00 2014 Subject: [Histonet] Ventana ultraview users Message-ID: Hello all, Our lab is getting ready to switch from using the iview DAB to the ultraview - any tips for validation? How many cases did you run for each antibody? Thanks! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 From Nancy_Schmitt <@t> pa-ucl.com Wed Jul 9 12:28:58 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Jul 9 12:29:04 2014 Subject: [Histonet] safety shower Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB26A748@PEITHA.wad.pa-ucl.com> How often are you testing your safety shower ? Are you always checking for water volume, temperature and clarity? I appreciate your thoughts., Nancy Schmitt MLT, HT(ASCP) Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From jshelley <@t> sanfordburnham.org Wed Jul 9 12:38:56 2014 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Wed Jul 9 12:39:05 2014 Subject: [Histonet] RE: safety shower In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB26A748@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB26A748@PEITHA.wad.pa-ucl.com> Message-ID: Our safety group here had built a neat little contraption using flexible PVC piping and making it circular, slightly larger than the shower head and placed a shower curtain and rings on it. When they pull the lever and use their system the water falls into a garbage can for collection. They come around once a month to check it out. Kind Regards! ? John J Shelley Research Specialist, Histology Core Facility -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, July 09, 2014 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] safety shower How often are you testing your safety shower ? Are you always checking for water volume, temperature and clarity? I appreciate your thoughts., Nancy Schmitt MLT, HT(ASCP) Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Jul 9 12:48:23 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jul 9 12:48:29 2014 Subject: [Histonet] RE: safety shower In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB26A748@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB26A748@PEITHA.wad.pa-ucl.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD2A7@SBS2K8.premierlab.local> We test ours weekly for flow along with our eye wash station. We purchased a device similar to what John built from Speakman Company (800) 537-2107 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, July 09, 2014 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] safety shower How often are you testing your safety shower ? Are you always checking for water volume, temperature and clarity? I appreciate your thoughts., Nancy Schmitt MLT, HT(ASCP) Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terra.Wineman <@t> novusint.com Wed Jul 9 13:35:58 2014 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Wed Jul 9 13:36:06 2014 Subject: [Histonet] RE: safety shower In-Reply-To: References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB26A748@PEITHA.wad.pa-ucl.com> Message-ID: <1EB8F245A303564EADF12AC7022FA74DB990917C@novus-ex01> Our facilities department checks the safety showers once a month as well. Hope this helps!! Terra Wineman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, July 09, 2014 12:39 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: safety shower Our safety group here had built a neat little contraption using flexible PVC piping and making it circular, slightly larger than the shower head and placed a shower curtain and rings on it. When they pull the lever and use their system the water falls into a garbage can for collection. They come around once a month to check it out. Kind Regards! ? John J Shelley Research Specialist, Histology Core Facility -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, July 09, 2014 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] safety shower How often are you testing your safety shower ? Are you always checking for water volume, temperature and clarity? I appreciate your thoughts., Nancy Schmitt MLT, HT(ASCP) Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ahc53 <@t> georgetown.edu Wed Jul 9 13:54:29 2014 From: ahc53 <@t> georgetown.edu (Anna Coffey) Date: Wed Jul 9 13:54:34 2014 Subject: [Histonet] Prioritizing Histology work/projects Message-ID: Hi Lesley, We are a research lab and we generally prioritize our work flow by date of submission. We occasionally batch things that have similar needs, such as decal for bone specimens or multiple cases in need of the same special stain. We also allow investigators to RUSH cases for a small fee if they have a pressing deadline like a manuscript resubmission or grant application. Hope this helps! Anna -- Anna Coffey, MS, HTL(ASCP) Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu Message: 5 Date: Tue, 8 Jul 2014 17:44:06 +0000 From: Lesley Bechtold Subject: [Histonet] Prioritizing Histology work/projects To: "histonet@lists.utsouthwestern.edu" Message-ID: <08997064E2075247A4DD5A035DB51CDF64FA5B9B@jaxbhexms02.jax.org> Content-Type: text/plain; charset="us-ascii" Good Afternoon, This may apply more to the research-type labs than clinical labs, but I am wondering: how do you prioritize the work that your Histology Lab or Service receives? Do you batch like things together? Do it strictly by date? Are there other ways to do it? I'd be interested in how it's done in other places. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From ehanson <@t> carisls.com Wed Jul 9 15:04:26 2014 From: ehanson <@t> carisls.com (Hanson, Eric) Date: Wed Jul 9 15:04:39 2014 Subject: [Histonet] RE: Prioritizing Histology work/projects In-Reply-To: <1404917258.12569.YahooMailNeo@web120405.mail.ne1.yahoo.com> References: <08997064E2075247A4DD5A035DB51CDF64FA5B9B@jaxbhexms02.jax.org> <1404917258.12569.YahooMailNeo@web120405.mail.ne1.yahoo.com> Message-ID: <03D535A75AB84D44AFC75161BE5BE6CF032A26A657@S-AZ-MLBX02.CLSNET.intranet> Thanks everyone for all the responses! I'm currently researching the options presented. Let me know if anybody else has any information that could help . Thanks, Eric Hanson CMI Histotech I Caris Life Sciences 4610 South 44th Place Phoenix, AZ 85040 ehanson@carisls.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, July 09, 2014 7:48 AM To: Roberta Horner; Lesley Bechtold; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Prioritizing Histology work/projects Regardless of what you are going to do with the samples later on or who is going to use them?for, you should always batch the samples of any given date and process them. Ren? J.? On Tuesday, July 8, 2014 2:07 PM, Roberta Horner wrote: I work for Penn State's Animal Diagnostic lab and I get diagnostic samples and research samples.? My priorities are diagnostic first then the research is done in the order it comes to the lab Roberta Horner Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Tuesday, July 08, 2014 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prioritizing Histology work/projects Good Afternoon, This may apply more to the research-type labs than clinical labs, but I am wondering: how do you prioritize the work that your Histology Lab or Service receives?? Do you batch like things together? Do it strictly by date?? Are there other ways to do it? I'd be interested in how it's done in other places. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ehanson <@t> carisls.com Wed Jul 9 15:32:05 2014 From: ehanson <@t> carisls.com (Hanson, Eric) Date: Wed Jul 9 15:32:24 2014 Subject: [Histonet] Bulk Tissue Procurement In-Reply-To: <1404916212.21333.YahooMailNeo@web120405.mail.ne1.yahoo.com> References: <03D535A75AB84D44AFC75161BE5BE6CF032A26A42A@S-AZ-MLBX02.CLSNET.intranet> <1404916212.21333.YahooMailNeo@web120405.mail.ne1.yahoo.com> Message-ID: <03D535A75AB84D44AFC75161BE5BE6CF032A26A689@S-AZ-MLBX02.CLSNET.intranet> I'm so sorry, I replied to the wrong email chain... Thanks everyone for all the responses! I'm currently researching the options presented. Let me know if anybody else has any information that could help . Thanks, Eric Hanson CMI Histotech I Caris Life Sciences 4610 South 44th Place Phoenix, AZ 85040 ehanson@carisls.com From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, July 09, 2014 7:30 AM To: Hanson, Eric; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Bulk Tissue Procurement Tissue vendors are very scarce and highly regulated to the point that such a "trade" is almost non-existent. I would advise you to get in contact with the Medical Examiner in your county and try to obtain tissue from them. Ren? J. On Wednesday, July 9, 2014 9:51 AM, "Hanson, Eric" > wrote: Good morning histonetters! We are looking for USA vendors for bulk tissue. Mostly we are short on HER2+ breast and cervical epidermal tissue (which is punchable, where skin is not). We make our own tissue microarray and also use gallbladder, prostate, uterus/myometrium, and kidney. Surely we are not the only lab that needs these tissue types, and I hope someone out there can point me in the direction of some new vendors. Thanks, Eric Hanson CMI Histotech I Caris Life Sciences 4610 South 44th Place Phoenix, AZ 85040 ehanson@carisls.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Wed Jul 9 18:35:12 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Wed Jul 9 18:35:19 2014 Subject: [Histonet] HTL wage range In-Reply-To: <468705ED810A34468A8650CFC201A800B45FD328@EWJMB001.EW.HSHSAD.ORG> References: <468705ED810A34468A8650CFC201A800B45FD328@EWJMB001.EW.HSHSAD.ORG> Message-ID: It's not so much about the money. It's more about the speedboats and supermodels. http://www.ascp.org/PDF/Advocacy/2012-Vacancy-Survey.pdf Sincerely, Jay A. Lundgren, M.S., HTL, (ASCP) On Tue, Jul 8, 2014 at 3:37 PM, Gast, Betty L wrote: > Hi, I was wondering if anyone would share your salary range for a new HTL > grad. I have the ASCP 2013 survey and wanted to compare. Thanks! > > > > Betty Gast HT(ASCP) > > betty.gast@hshs.org > > Histology Facilitator > > St. Mary's PAthology Department > > Green Bay, WI 54303 > > > > LEGAL DISCLAIMER: This message and all attachments may be confidential or > protected by privilege. If you are not the intended recipient, you are > hereby notified that any disclosure, copying, distribution or use of the > information contained in or attached to this message is strictly > prohibited. Please notify the sender of the delivery error by replying to > this message, and then delete it from your system. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bburnett <@t> CapeCodHealth.org Thu Jul 10 11:08:09 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Thu Jul 10 11:08:32 2014 Subject: [Histonet] Cytokeratin AE1/AE3 Message-ID: Hi Histonetters, I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. I was wondering if anyone else has come across this, and if they have fixed it? Thank you, Brandy Burnett, HTL Cape Cod Hospital ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From cward <@t> cbgbiotech.com Thu Jul 10 12:01:36 2014 From: cward <@t> cbgbiotech.com (Cary Ward, Technical Support Representative) Date: Thu Jul 10 12:02:34 2014 Subject: [Histonet] out of office reply Message-ID: I will be out of the office on Thursday 7/10. If you need assistance, please contact Sue Bauer at sbauer@cbgbiotech.com or x203. Regards, Cary Ward Technical Support Respresentative 800-941-9484, ext 220 cward@cbgbiotech.com From KSimeone <@t> leavittmgt.com Thu Jul 10 12:26:52 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Thu Jul 10 12:26:57 2014 Subject: [Histonet] FT DERM POSITION DELRAY BEACH, FLORIDA Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C2FD9FC68@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Derm Lab. This is a permanent full time position with benefits (medical/401k/vacation). ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann@leavittmgt.com if interested. *full time position Mon-Fri 11a-7:30p (hours NON NEGOTIABLE) *MUST be licensed as a FL histotechnologist or technician *MUST have at least 2 years experience (dermatology preferred) *VERY proficient in embedding and microtomy *experience in grossing and immunohistochemistry (BOND) a plus *must be self motivated and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From bethcoxx <@t> gmail.com Thu Jul 10 12:56:42 2014 From: bethcoxx <@t> gmail.com (Beth Cox) Date: Thu Jul 10 12:56:43 2014 Subject: [Histonet] Re: Ventana Ultraview users Message-ID: <53BED3DA.5070300@gmail.com> Beth, We are doing exactly that same thing right now. This is changing a complete detection system, so we need a full validation (10 pos + 10 negs for all non-prognostic markers). To avoid running 20 slides on every antibody on our menu ($$$$), we have chosen to use a 3-prong method: 1. we bought appropriate TMAs from reputable vendors (such as Pantomics, Inc) which will provide more than 10 Pos +10 neg for most cases. 2. we made a universal "negative" control with 12 different tissues from our collection (provides tissues processed by us) 3. we run 1(or 2) of our control slides (bonus: also validates the control at the same time) This way we only run three slides for each validation to get the required number of tissues - big difference in workload and cost! Beth Cox, HTL/SCT(ASCP)QIHC Pathology Solutions, Inc __________________________________________________________________________ Message: 1 Date: Wed, 9 Jul 2014 12:16:55 -0500 From: Beth Brinegar Subject: [Histonet] Ventana ultraview users To:"histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Hello all, Our lab is getting ready to switch from using the iview DAB to the ultraview - any tips for validation? How many cases did you run for each antibody? Thanks! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 From jfray80 <@t> hotmail.com Thu Jul 10 14:12:31 2014 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Thu Jul 10 14:12:36 2014 Subject: [Histonet] Paraffin blocks Message-ID: How is everyone disposing of paraffin blocks regular trash or biohazard containers From mroark <@t> sfmc.net Thu Jul 10 14:16:02 2014 From: mroark <@t> sfmc.net (Matthew D. Roark) Date: Thu Jul 10 14:16:12 2014 Subject: [Histonet] Paraffin blocks In-Reply-To: References: Message-ID: <6db7b87e8a7e463dbc09b07eee04f377@BLUPR04MB199.namprd04.prod.outlook.com> We are currently using biohazard containers. Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE Sent: Thursday, July 10, 2014 2:13 PM To: histonet server Subject: [Histonet] Paraffin blocks How is everyone disposing of paraffin blocks regular trash or biohazard containers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Thu Jul 10 14:23:11 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Jul 10 14:23:21 2014 Subject: [Histonet] Paraffin blocks In-Reply-To: References: Message-ID: In every institution I've ever worked, paraffin tissue blocks were handled as regulated medical waste, and therefore discarded into red biohazard bags. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE Sent: Thursday, July 10, 2014 12:13 PM To: histonet server Subject: [Histonet] Paraffin blocks How is everyone disposing of paraffin blocks regular trash or biohazard containers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Jul 10 14:54:34 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Jul 10 14:54:40 2014 Subject: [Histonet] RE: Cytokeratin AE1/AE3 In-Reply-To: References: Message-ID: <1405022056378.36433@accuratediagnosticlabs.com> What are you using for HIER? Citrate buffer? What pH? I use a low pH citrate buffer at 95C for 20 minutes and get good results. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Burnett, Brandy Sent: Thursday, July 10, 2014 3:08 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cytokeratin AE1/AE3 Hi Histonetters, I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. I was wondering if anyone else has come across this, and if they have fixed it? Thank you, Brandy Burnett, HTL Cape Cod Hospital ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis <@t> baylorhealth.edu Thu Jul 10 14:58:19 2014 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Thu Jul 10 14:58:34 2014 Subject: [Histonet] Baylor University Medical Center Job Openings Message-ID: <2572B4D63B62E64A8078D8BBE34D407801B660B4@BHDASVEXML2.bhcs.pvt> Good Afternoon. I just wanted to let everyone know that Baylor University Medical Center Dallas has 2 job opening for Histotechnician Sr. One of my current technicians is moving into our new Research position leaving his position open. We also have been allowed to change 2 part time positions that were open into 1 full time position. If you are interested in apply for either of our position you can do so via internet at www.baylorhealth.com/Careers/Career_Fields/Pages/Laboratory.aspx You must enter the job requisition numbers when applying. Requisition numbers are 184620 and 184554. Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Richard.Cartun <@t> hhchealth.org Thu Jul 10 15:07:42 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Jul 10 15:07:51 2014 Subject: [Histonet] RE: Cytokeratin AE1/AE3 In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355B571B@HHCEXCHMB05.hhcsystem.org> "Extra-follicular reticulm cells" contain cytokeratin and will label with antibodies to low molecular weight cytokeratin. Could that be what you are seeing? Reference: Linden MD and Zarbo RJ: Cytokeratin immunostaining patterns of benign, reactive lymph nodes: applications for the evaluation of sentinel lymph node specimen. Appl Immunohistochem Mol Morphol 2001;9(4):297-301. On another note, the recommendation now is to not do CK IHC on sentinel lymph nodes (except for rare cases). Lymph node involvement should be based on H&E; not IHC. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, July 10, 2014 12:08 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cytokeratin AE1/AE3 Hi Histonetters, I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. I was wondering if anyone else has come across this, and if they have fixed it? Thank you, Brandy Burnett, HTL Cape Cod Hospital ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From vrivera <@t> westderm.com Thu Jul 10 15:12:03 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Thu Jul 10 15:12:17 2014 Subject: [Histonet] Paraffin blocks In-Reply-To: References: Message-ID: <3D4A471B82E7A44C87F6839732320D9F013F32C206@VSPDMS-ITEXMB02.DMS.COM> I know this is slightly off topic but still along the same lines. How is everybody handling their paraffin shaving waste? The waste that is produced when trimming/facing tissue blocks? Is this also considered regulated medical waste? Thanks, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Thursday, July 10, 2014 12:23 PM To: JOSEPH FRAZEE; histonet server Subject: RE: [Histonet] Paraffin blocks In every institution I've ever worked, paraffin tissue blocks were handled as regulated medical waste, and therefore discarded into red biohazard bags. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE Sent: Thursday, July 10, 2014 12:13 PM To: histonet server Subject: [Histonet] Paraffin blocks How is everyone disposing of paraffin blocks regular trash or biohazard containers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Thu Jul 10 15:29:31 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Jul 10 15:29:42 2014 Subject: [Histonet] Paraffin blocks In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F013F32C206@VSPDMS-ITEXMB02.DMS.COM> References: <3D4A471B82E7A44C87F6839732320D9F013F32C206@VSPDMS-ITEXMB02.DMS.COM> Message-ID: Good question. So the linear path would dictate that they too should be discarded into red bags, right? You could also pose the same question about the kimwipes used to skim your flotation baths. We've always just thrown both the shavings and kimwipes into the regular trash though. Guess we're not so linear . . . Thanks, Brian -----Original Message----- From: Vincent Rivera [mailto:vrivera@westderm.com] Sent: Thursday, July 10, 2014 1:12 PM To: Cooper, Brian; JOSEPH FRAZEE; histonet server Subject: RE: [Histonet] Paraffin blocks I know this is slightly off topic but still along the same lines. How is everybody handling their paraffin shaving waste? The waste that is produced when trimming/facing tissue blocks? Is this also considered regulated medical waste? Thanks, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Thursday, July 10, 2014 12:23 PM To: JOSEPH FRAZEE; histonet server Subject: RE: [Histonet] Paraffin blocks In every institution I've ever worked, paraffin tissue blocks were handled as regulated medical waste, and therefore discarded into red biohazard bags. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE Sent: Thursday, July 10, 2014 12:13 PM To: histonet server Subject: [Histonet] Paraffin blocks How is everyone disposing of paraffin blocks regular trash or biohazard containers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From vrivera <@t> westderm.com Thu Jul 10 15:37:39 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Thu Jul 10 15:37:44 2014 Subject: [Histonet] Paraffin blocks In-Reply-To: References: <3D4A471B82E7A44C87F6839732320D9F013F32C206@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <3D4A471B82E7A44C87F6839732320D9F013F32C253@VSPDMS-ITEXMB02.DMS.COM> You would think, especially when you see the amount of waste a tech can produce on "high volume" days, but we also discard ours into regular trash too. Just curious how other facilities handle this type of waste. Regards, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: Cooper, Brian [mailto:bcooper@chla.usc.edu] Sent: Thursday, July 10, 2014 1:30 PM To: Vincent Rivera; JOSEPH FRAZEE; histonet server Subject: RE: [Histonet] Paraffin blocks Good question. So the linear path would dictate that they too should be discarded into red bags, right? You could also pose the same question about the kimwipes used to skim your flotation baths. We've always just thrown both the shavings and kimwipes into the regular trash though. Guess we're not so linear . . . Thanks, Brian -----Original Message----- From: Vincent Rivera [mailto:vrivera@westderm.com] Sent: Thursday, July 10, 2014 1:12 PM To: Cooper, Brian; JOSEPH FRAZEE; histonet server Subject: RE: [Histonet] Paraffin blocks I know this is slightly off topic but still along the same lines. How is everybody handling their paraffin shaving waste? The waste that is produced when trimming/facing tissue blocks? Is this also considered regulated medical waste? Thanks, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Thursday, July 10, 2014 12:23 PM To: JOSEPH FRAZEE; histonet server Subject: RE: [Histonet] Paraffin blocks In every institution I've ever worked, paraffin tissue blocks were handled as regulated medical waste, and therefore discarded into red biohazard bags. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE Sent: Thursday, July 10, 2014 12:13 PM To: histonet server Subject: [Histonet] Paraffin blocks How is everyone disposing of paraffin blocks regular trash or biohazard containers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Toni.Rathborne <@t> rwjuh.edu Thu Jul 10 15:49:13 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Thu Jul 10 15:49:23 2014 Subject: [Histonet] RE: Cytokeratin AE1/AE3 In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355B571B@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E355B571B@HHCEXCHMB05.hhcsystem.org> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24EAAC89@vap1013.win.rwjuh.edu> Would you share the source for the sentinel lymph node recommendation mentioned? Although I just did a quick search, I didn't see anything on the CAP website. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, July 10, 2014 4:08 PM To: Burnett, Brandy; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Cytokeratin AE1/AE3 "Extra-follicular reticulm cells" contain cytokeratin and will label with antibodies to low molecular weight cytokeratin. Could that be what you are seeing? Reference: Linden MD and Zarbo RJ: Cytokeratin immunostaining patterns of benign, reactive lymph nodes: applications for the evaluation of sentinel lymph node specimen. Appl Immunohistochem Mol Morphol 2001;9(4):297-301. On another note, the recommendation now is to not do CK IHC on sentinel lymph nodes (except for rare cases). Lymph node involvement should be based on H&E; not IHC. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, July 10, 2014 12:08 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cytokeratin AE1/AE3 Hi Histonetters, I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. I was wondering if anyone else has come across this, and if they have fixed it? Thank you, Brandy Burnett, HTL Cape Cod Hospital ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Thu Jul 10 15:56:02 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu Jul 10 15:56:07 2014 Subject: [Histonet] Paraffin blocks In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F013F32C253@VSPDMS-ITEXMB02.DMS.COM> References: , , <3D4A471B82E7A44C87F6839732320D9F013F32C206@VSPDMS-ITEXMB02.DMS.COM>, , <3D4A471B82E7A44C87F6839732320D9F013F32C253@VSPDMS-ITEXMB02.DMS.COM> Message-ID: I have been following this stream of conversation and thought I might jump in. Paraffin blocks and microtomy waste are NOT biohazard waste. The process of fixation and tissue processing makes the tissue acceptable for general waste disposal. You do have to address the issue of PHI on the blocks, if you are using bar code information and especially if patient information is included in the bar code. All bar codes used in the lab can be read in any standard word or spreadsheet software. I do not see the need to pay for expensive removal of biohazard waste, use general waste disposal for your blocks and cutting waste. William DeSalvo, BS HTL(ASCP) > From: vrivera@westderm.com > To: bcooper@chla.usc.edu; jfray80@hotmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 10 Jul 2014 20:37:39 +0000 > Subject: RE: [Histonet] Paraffin blocks > CC: > > You would think, especially when you see the amount of waste a tech can produce on "high volume" days, but we also discard ours into regular trash too. Just curious how other facilities handle this type of waste. > > Regards, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: Cooper, Brian [mailto:bcooper@chla.usc.edu] > Sent: Thursday, July 10, 2014 1:30 PM > To: Vincent Rivera; JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > Good question. So the linear path would dictate that they too should be discarded into red bags, right? You could also pose the same question about the kimwipes used to skim your flotation baths. We've always just thrown both the shavings and kimwipes into the regular trash though. Guess we're not so linear . . . > > Thanks, > > Brian > > > -----Original Message----- > From: Vincent Rivera [mailto:vrivera@westderm.com] > Sent: Thursday, July 10, 2014 1:12 PM > To: Cooper, Brian; JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > I know this is slightly off topic but still along the same lines. How is everybody handling their paraffin shaving waste? The waste that is produced when trimming/facing tissue blocks? Is this also considered regulated medical waste? > > Thanks, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian > Sent: Thursday, July 10, 2014 12:23 PM > To: JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > In every institution I've ever worked, paraffin tissue blocks were handled as regulated medical waste, and therefore discarded into red biohazard bags. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE > Sent: Thursday, July 10, 2014 12:13 PM > To: histonet server > Subject: [Histonet] Paraffin blocks > > How is everyone disposing of paraffin blocks regular trash or biohazard containers > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet locks and cutting waste From Toni.Rathborne <@t> rwjuh.edu Thu Jul 10 16:05:12 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Thu Jul 10 16:05:20 2014 Subject: [Histonet] Paraffin blocks In-Reply-To: References: , , <3D4A471B82E7A44C87F6839732320D9F013F32C206@VSPDMS-ITEXMB02.DMS.COM>, , <3D4A471B82E7A44C87F6839732320D9F013F32C253@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24EABCA4@vap1013.win.rwjuh.edu> Ours are incinerated because of the PHI. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, July 10, 2014 4:56 PM To: Vincent Rivera; 'Cooper, Brian'; JOSEPH FRAZEE; histonet Subject: RE: [Histonet] Paraffin blocks I have been following this stream of conversation and thought I might jump in. Paraffin blocks and microtomy waste are NOT biohazard waste. The process of fixation and tissue processing makes the tissue acceptable for general waste disposal. You do have to address the issue of PHI on the blocks, if you are using bar code information and especially if patient information is included in the bar code. All bar codes used in the lab can be read in any standard word or spreadsheet software. I do not see the need to pay for expensive removal of biohazard waste, use general waste disposal for your blocks and cutting waste. William DeSalvo, BS HTL(ASCP) > From: vrivera@westderm.com > To: bcooper@chla.usc.edu; jfray80@hotmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 10 Jul 2014 20:37:39 +0000 > Subject: RE: [Histonet] Paraffin blocks > CC: > > You would think, especially when you see the amount of waste a tech can produce on "high volume" days, but we also discard ours into regular trash too. Just curious how other facilities handle this type of waste. > > Regards, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: Cooper, Brian [mailto:bcooper@chla.usc.edu] > Sent: Thursday, July 10, 2014 1:30 PM > To: Vincent Rivera; JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > Good question. So the linear path would dictate that they too should be discarded into red bags, right? You could also pose the same question about the kimwipes used to skim your flotation baths. We've always just thrown both the shavings and kimwipes into the regular trash though. Guess we're not so linear . . . > > Thanks, > > Brian > > > -----Original Message----- > From: Vincent Rivera [mailto:vrivera@westderm.com] > Sent: Thursday, July 10, 2014 1:12 PM > To: Cooper, Brian; JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > I know this is slightly off topic but still along the same lines. How is everybody handling their paraffin shaving waste? The waste that is produced when trimming/facing tissue blocks? Is this also considered regulated medical waste? > > Thanks, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian > Sent: Thursday, July 10, 2014 12:23 PM > To: JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > In every institution I've ever worked, paraffin tissue blocks were handled as regulated medical waste, and therefore discarded into red biohazard bags. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE > Sent: Thursday, July 10, 2014 12:13 PM > To: histonet server > Subject: [Histonet] Paraffin blocks > > How is everyone disposing of paraffin blocks regular trash or biohazard containers > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet locks and cutting waste _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vrivera <@t> westderm.com Thu Jul 10 16:07:00 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Thu Jul 10 16:07:10 2014 Subject: [Histonet] Paraffin blocks In-Reply-To: References: , , <3D4A471B82E7A44C87F6839732320D9F013F32C206@VSPDMS-ITEXMB02.DMS.COM>, , <3D4A471B82E7A44C87F6839732320D9F013F32C253@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <3D4A471B82E7A44C87F6839732320D9F013F32C2D8@VSPDMS-ITEXMB02.DMS.COM> Interesting point. Thank you for chiming in on this Mr. Desalvo Best, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) From: WILLIAM DESALVO [mailto:wdesalvo.cac@outlook.com] Sent: Thursday, July 10, 2014 1:56 PM To: Vincent Rivera; 'Cooper, Brian'; JOSEPH FRAZEE; histonet Subject: RE: [Histonet] Paraffin blocks I have been following this stream of conversation and thought I might jump in. Paraffin blocks and microtomy waste are NOT biohazard waste. The process of fixation and tissue processing makes the tissue acceptable for general waste disposal. You do have to address the issue of PHI on the blocks, if you are using bar code information and especially if patient information is included in the bar code. All bar codes used in the lab can be read in any standard word or spreadsheet software. I do not see the need to pay for expensive removal of biohazard waste, use general waste disposal for your blocks and cutting waste. William DeSalvo, BS HTL(ASCP) > From: vrivera@westderm.com > To: bcooper@chla.usc.edu; jfray80@hotmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 10 Jul 2014 20:37:39 +0000 > Subject: RE: [Histonet] Paraffin blocks > CC: > > You would think, especially when you see the amount of waste a tech can produce on "high volume" days, but we also discard ours into regular trash too. Just curious how other facilities handle this type of waste. > > Regards, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: Cooper, Brian [mailto:bcooper@chla.usc.edu] > Sent: Thursday, July 10, 2014 1:30 PM > To: Vincent Rivera; JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > Good question. So the linear path would dictate that they too should be discarded into red bags, right? You could also pose the same question about the kimwipes used to skim your flotation baths. We've always just thrown both the shavings and kimwipes into the regular trash though. Guess we're not so linear . . . > > Thanks, > > Brian > > > -----Original Message----- > From: Vincent Rivera [mailto:vrivera@westderm.com] > Sent: Thursday, July 10, 2014 1:12 PM > To: Cooper, Brian; JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > I know this is slightly off topic but still along the same lines. How is everybody handling their paraffin shaving waste? The waste that is produced when trimming/facing tissue blocks? Is this also considered regulated medical waste? > > Thanks, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian > Sent: Thursday, July 10, 2014 12:23 PM > To: JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > In every institution I've ever worked, paraffin tissue blocks were handled as regulated medical waste, and therefore discarded into red biohazard bags. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE > Sent: Thursday, July 10, 2014 12:13 PM > To: histonet server > Subject: [Histonet] Paraffin blocks > > How is everyone disposing of paraffin blocks regular trash or biohazard containers > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet locks and cutting waste From akbitting <@t> geisinger.edu Thu Jul 10 16:09:12 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Jul 10 16:09:19 2014 Subject: [Histonet] Paraffin blocks In-Reply-To: References: , , <3D4A471B82E7A44C87F6839732320D9F013F32C206@VSPDMS-ITEXMB02.DMS.COM>, , <3D4A471B82E7A44C87F6839732320D9F013F32C253@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F679E508AB@GHSEXMBX1W8K1V.geisinger.edu> Our facility handles paraffin shavings and blocks as Biological Waste which is different from Biohazardous waste. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, July 10, 2014 4:56 PM To: Vincent Rivera; 'Cooper, Brian'; JOSEPH FRAZEE; histonet Subject: RE: [Histonet] Paraffin blocks I have been following this stream of conversation and thought I might jump in. Paraffin blocks and microtomy waste are NOT biohazard waste. The process of fixation and tissue processing makes the tissue acceptable for general waste disposal. You do have to address the issue of PHI on the blocks, if you are using bar code information and especially if patient information is included in the bar code. All bar codes used in the lab can be read in any standard word or spreadsheet software. I do not see the need to pay for expensive removal of biohazard waste, use general waste disposal for your blocks and cutting waste. William DeSalvo, BS HTL(ASCP) > From: vrivera@westderm.com > To: bcooper@chla.usc.edu; jfray80@hotmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 10 Jul 2014 20:37:39 +0000 > Subject: RE: [Histonet] Paraffin blocks > CC: > > You would think, especially when you see the amount of waste a tech can produce on "high volume" days, but we also discard ours into regular trash too. Just curious how other facilities handle this type of waste. > > Regards, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: Cooper, Brian [mailto:bcooper@chla.usc.edu] > Sent: Thursday, July 10, 2014 1:30 PM > To: Vincent Rivera; JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > Good question. So the linear path would dictate that they too should be discarded into red bags, right? You could also pose the same question about the kimwipes used to skim your flotation baths. We've always just thrown both the shavings and kimwipes into the regular trash though. Guess we're not so linear . . . > > Thanks, > > Brian > > > -----Original Message----- > From: Vincent Rivera [mailto:vrivera@westderm.com] > Sent: Thursday, July 10, 2014 1:12 PM > To: Cooper, Brian; JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > I know this is slightly off topic but still along the same lines. How is everybody handling their paraffin shaving waste? The waste that is produced when trimming/facing tissue blocks? Is this also considered regulated medical waste? > > Thanks, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian > Sent: Thursday, July 10, 2014 12:23 PM > To: JOSEPH FRAZEE; histonet server > Subject: RE: [Histonet] Paraffin blocks > > In every institution I've ever worked, paraffin tissue blocks were handled as regulated medical waste, and therefore discarded into red biohazard bags. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles bcooper@chla.usc.edu > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JOSEPH FRAZEE > Sent: Thursday, July 10, 2014 12:13 PM > To: histonet server > Subject: [Histonet] Paraffin blocks > > How is everyone disposing of paraffin blocks regular trash or biohazard containers > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet locks and cutting waste _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From amber.mckenzie <@t> gastrodocs.net Fri Jul 11 09:36:14 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Jul 11 09:36:19 2014 Subject: [Histonet] RE: safety shower In-Reply-To: References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36FB26A748@PEITHA.wad.pa-ucl.com> Message-ID: <5A33C952BB67F4468AF1F36D739212BC011250E32A@JERRY.Gia.com> What should the water volume be? Temperature and clarity? How do you test these things? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley Sent: Wednesday, July 09, 2014 12:39 PM To: Nancy Schmitt; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: safety shower Our safety group here had built a neat little contraption using flexible PVC piping and making it circular, slightly larger than the shower head and placed a shower curtain and rings on it. When they pull the lever and use their system the water falls into a garbage can for collection. They come around once a month to check it out. Kind Regards! ? John J Shelley Research Specialist, Histology Core Facility -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, July 09, 2014 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] safety shower How often are you testing your safety shower ? Are you always checking for water volume, temperature and clarity? I appreciate your thoughts., Nancy Schmitt MLT, HT(ASCP) Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbaldwin <@t> mhhcc.org Fri Jul 11 09:49:35 2014 From: sbaldwin <@t> mhhcc.org (Baldwin, Kathy) Date: Fri Jul 11 09:49:42 2014 Subject: [Histonet] Job Opportunity Message-ID: <9e3d7cb10c43496c8e91f4e30af8a1bf@exch02.mhhcc.org> Histology Tech position: Full time Monday thru Friday. Jasper, Indiana 47546Jasper is a regional center in southwestern Indiana, noted for its German Catholic ancestral roots Jasper has often been called the "Wood Capital of the World", boasting a large number of furniture companies, including Kimball International and Masterbrand Cabinets. Jasper is also home to the Southern Indiana Education Service Center (SIEC), Jasper Engines & Transmissions (largest remanufacturer in the market), and to a satellite campus of Vincennes University. JOB SUMMARY: Responsible for embedding, sectioning and cover slipping paraffin blocks and staining tissues to provide the pathologist with accurately prepared specimens for review and diagnosis. The employee works with specimens from patients of all ages. The employee must reflect mission statement and philosophy of Memorial Hospital and Health Care Center in daily work habits and contacts. QUALIFICATIONS: Education: High school diploma or equivalent is required. Associate degree preferred. HT (ASCP) certification is preferred. Three years' experience as histology assist is acceptable. Training: Six months on the job training required. Experience: Previous histology experience is preferred. New graduates of histology school will be considered. S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbaldwin@mhhcc.org "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" From Richard.Cartun <@t> hhchealth.org Fri Jul 11 11:08:56 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Jul 11 11:09:03 2014 Subject: [Histonet] RE: Cytokeratin AE1/AE3 Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355B5979@HHCEXCHMB05.hhcsystem.org> Reference: Weaver DL, et al.: Effect of occult metastases on survival in node-negative breast cancer. NEJM 2011;364:412-421. A study funded by the National Cancer Institute (Clinical Trials). Conclusion - "These data do not indicate a clinical benefit of additional evaluation, including immunohistochemical analysis, of initially negative sentinel nodes in patients with breast cancer. Also, from an NSABP study: Role of IHC in invasive lobular carcinoma - Although routine IHC staining with cytokeratin is not indicated for most breast cancers, it can be helpful for examination of the sentinel nodes in patients with invasive lobular carcinoma since the morphology of lobular cancer can be difficult to detect on H&E of axillary lymph nodes [91,92]. In general, IHC should be used to definitively diagnose an area that is suspicious for, but not diagnostic of, lymph node metastases on H&E rather than as a routine method of evaluating nodes in cases of invasive lobular cancer. I believe most major cancer centers have stopped doing cytokeratin IHC on sentinel nodes for most breast cancer specimens. I know that we have. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: Rathborne, Toni [mailto:Toni.Rathborne@rwjuh.edu] Sent: Thursday, July 10, 2014 4:49 PM To: Cartun, Richard; Burnett, Brandy; 'histonet@lists.utsouthwestern.edu' Subject: RE: Cytokeratin AE1/AE3 Would you share the source for the sentinel lymph node recommendation mentioned? Although I just did a quick search, I didn't see anything on the CAP website. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, July 10, 2014 4:08 PM To: Burnett, Brandy; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Cytokeratin AE1/AE3 "Extra-follicular reticulm cells" contain cytokeratin and will label with antibodies to low molecular weight cytokeratin. Could that be what you are seeing? Reference: Linden MD and Zarbo RJ: Cytokeratin immunostaining patterns of benign, reactive lymph nodes: applications for the evaluation of sentinel lymph node specimen. Appl Immunohistochem Mol Morphol 2001;9(4):297-301. On another note, the recommendation now is to not do CK IHC on sentinel lymph nodes (except for rare cases). Lymph node involvement should be based on H&E; not IHC. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, July 10, 2014 12:08 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cytokeratin AE1/AE3 Hi Histonetters, I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. I was wondering if anyone else has come across this, and if they have fixed it? Thank you, Brandy Burnett, HTL Cape Cod Hospital ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmire <@t> cvpath.org Fri Jul 11 11:41:04 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Fri Jul 11 11:41:12 2014 Subject: [Histonet] formalin/xylene monitoring Message-ID: <8ECF026F-6B02-4483-8D66-8CEF31E5A323@cvpath.org> Hello Netters I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. Ronda Mire CVPath Institute Gaithersburg, MD From vrivera <@t> westderm.com Fri Jul 11 11:49:10 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Fri Jul 11 11:49:20 2014 Subject: [Histonet] formalin/xylene monitoring In-Reply-To: <8ECF026F-6B02-4483-8D66-8CEF31E5A323@cvpath.org> References: <8ECF026F-6B02-4483-8D66-8CEF31E5A323@cvpath.org> Message-ID: <3D4A471B82E7A44C87F6839732320D9F013F32C582@VSPDMS-ITEXMB02.DMS.COM> Hi Ronda, It is located in the Anatomic Pathology Checklist safety section, I believe. Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire Sent: Friday, July 11, 2014 9:41 AM To: Subject: [Histonet] formalin/xylene monitoring Hello Netters I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. Ronda Mire CVPath Institute Gaithersburg, MD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmire <@t> cvpath.org Fri Jul 11 11:57:09 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Fri Jul 11 11:57:19 2014 Subject: [Histonet] formalin/xylene monitoring In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F013F32C582@VSPDMS-ITEXMB02.DMS.COM> References: <8ECF026F-6B02-4483-8D66-8CEF31E5A323@cvpath.org> <3D4A471B82E7A44C87F6839732320D9F013F32C582@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <87801E1B-1FAD-46CB-908C-F97C257E18A1@cvpath.org> Found it, Thanks !!! On Jul 11, 2014, at 12:49 PM, Vincent Rivera wrote: > Hi Ronda, > > It is located in the Anatomic Pathology Checklist safety section, I believe. > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire > Sent: Friday, July 11, 2014 9:41 AM > To: > Subject: [Histonet] formalin/xylene monitoring > > > Hello Netters > > I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. > > Ronda Mire > CVPath Institute > Gaithersburg, MD > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rmire <@t> cvpath.org Fri Jul 11 12:01:42 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Fri Jul 11 12:01:55 2014 Subject: Fwd: [Histonet] formalin/xylene monitoring References: <8ECF026F-6B02-4483-8D66-8CEF31E5A323@cvpath.org> Message-ID: <8909E44B-99CC-4161-8021-301438E0D99A@cvpath.org> Found it !! Thanks Begin forwarded message: > From: Ronda Mire > Subject: [Histonet] formalin/xylene monitoring > Date: July 11, 2014 12:41:04 PM EDT > To: "" > > > Hello Netters > > I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. > > Ronda Mire > CVPath Institute > Gaithersburg, MD > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aliciaroselange <@t> gmail.com Fri Jul 11 12:30:19 2014 From: aliciaroselange <@t> gmail.com (Alicia Lange) Date: Fri Jul 11 12:30:45 2014 Subject: [Histonet] Safety shower In-Reply-To: <53c01826.e762b60a.6173.3745SMTPIN_ADDED_MISSING@mx.google.com> References: <53c01826.e762b60a.6173.3745SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: I test our shower and eye wash weekly. We had a consulting company prepare us for an OSHA inspection and we were told then the testing needed to be done weekly for both. The temperate, flow, and clarity aren't necessarily tested just function. I use a 35 gallon trash bin to collect the water from the shower. I only run the shower enough to flush about 5 gallons out. Hope this helps! Alicia Lange HTL (ASCP) > On Jul 11, 2014, at 10:00 AM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: safety shower (Amber McKenzie) > 2. Job Opportunity (Baldwin, Kathy) > 3. RE: Cytokeratin AE1/AE3 (Cartun, Richard) > 4. formalin/xylene monitoring (Ronda Mire) > 5. RE: formalin/xylene monitoring (Vincent Rivera) > 6. Re: formalin/xylene monitoring (Ronda Mire) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 11 Jul 2014 14:36:14 +0000 > From: Amber McKenzie > Subject: [Histonet] RE: safety shower > To: John Shelley , Nancy Schmitt > , "histonet@lists.utsouthwestern.edu" > > Message-ID: <5A33C952BB67F4468AF1F36D739212BC011250E32A@JERRY.Gia.com> > Content-Type: text/plain; charset="iso-8859-1" > > What should the water volume be? Temperature and clarity? How do you test these things? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Shelley > Sent: Wednesday, July 09, 2014 12:39 PM > To: Nancy Schmitt; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: safety shower > > Our safety group here had built a neat little contraption using flexible PVC piping and making it circular, slightly larger than the shower head and placed a shower curtain and rings on it. When they pull the lever and use their system the water falls into a garbage can for collection. They come around once a month to check it out. > > Kind Regards! > > John J Shelley > Research Specialist, Histology Core Facility > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt > Sent: Wednesday, July 09, 2014 1:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] safety shower > > How often are you testing your safety shower ? Are you always checking for water volume, temperature and clarity? > > I appreciate your thoughts., > > Nancy Schmitt MLT, HT(ASCP) > Dubuque, IA > > > > NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Fri, 11 Jul 2014 14:49:35 +0000 > From: "Baldwin, Kathy" > Subject: [Histonet] Job Opportunity > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: <9e3d7cb10c43496c8e91f4e30af8a1bf@exch02.mhhcc.org> > Content-Type: text/plain; charset="us-ascii" > > Histology Tech position: Full time Monday thru Friday. Jasper, Indiana 47546Jasper is a regional center in southwestern Indiana, noted for its German Catholic ancestral roots Jasper has often been called the "Wood Capital of the World", boasting a large number of furniture companies, including Kimball International and Masterbrand Cabinets. Jasper is also home to the Southern Indiana Education Service Center (SIEC), Jasper Engines & Transmissions (largest remanufacturer in the market), and to a satellite campus of Vincennes University. > > JOB SUMMARY: > > Responsible for embedding, sectioning and cover slipping paraffin blocks and staining tissues to provide the pathologist with accurately prepared specimens for review and diagnosis. The employee works with specimens from patients of all ages. The employee must reflect mission statement and philosophy of Memorial Hospital and Health Care Center in daily work habits and contacts. > QUALIFICATIONS: > > Education: High school diploma or equivalent is required. Associate degree preferred. HT (ASCP) certification is preferred. Three years' experience as histology assist is acceptable. > > Training: Six months on the job training required. > > Experience: Previous histology experience is preferred. New graduates of histology school will be considered. > > S Kathy Baldwin > Histology/Cytology Supervisor > PH. 812-996-0210, Fax 812-996-0232 > sbaldwin@mhhcc.org > > "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" > > > > ------------------------------ > > Message: 3 > Date: Fri, 11 Jul 2014 16:08:56 +0000 > From: "Cartun, Richard" > Subject: [Histonet] RE: Cytokeratin AE1/AE3 > To: "Rathborne, Toni" , "Burnett, Brandy" > , "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2E355B5979@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="us-ascii" > > Reference: Weaver DL, et al.: Effect of occult metastases on survival in node-negative breast cancer. NEJM 2011;364:412-421. A study funded by the National Cancer Institute (Clinical Trials). > > Conclusion - "These data do not indicate a clinical benefit of additional evaluation, including immunohistochemical analysis, of initially negative sentinel nodes in patients with breast cancer. > > Also, from an NSABP study: Role of IHC in invasive lobular carcinoma - Although routine IHC staining with cytokeratin is not indicated for most breast cancers, it can be helpful for examination of the sentinel nodes in patients with invasive lobular carcinoma since the morphology of lobular cancer can be difficult to detect on H&E of axillary lymph nodes [91,92]. In general, IHC should be used to definitively diagnose an area that is suspicious for, but not diagnostic of, lymph node metastases on H&E rather than as a routine method of evaluating nodes in cases of invasive lobular cancer. > > I believe most major cancer centers have stopped doing cytokeratin IHC on sentinel nodes for most breast cancer specimens. I know that we have. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: Rathborne, Toni [mailto:Toni.Rathborne@rwjuh.edu] > Sent: Thursday, July 10, 2014 4:49 PM > To: Cartun, Richard; Burnett, Brandy; 'histonet@lists.utsouthwestern.edu' > Subject: RE: Cytokeratin AE1/AE3 > > Would you share the source for the sentinel lymph node recommendation mentioned? Although I just did a quick search, I didn't see anything on the CAP website. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard > Sent: Thursday, July 10, 2014 4:08 PM > To: Burnett, Brandy; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Cytokeratin AE1/AE3 > > "Extra-follicular reticulm cells" contain cytokeratin and will label with antibodies to low molecular weight cytokeratin. Could that be what you are seeing? > > Reference: Linden MD and Zarbo RJ: Cytokeratin immunostaining patterns of benign, reactive lymph nodes: applications for the evaluation of sentinel lymph node specimen. Appl Immunohistochem Mol Morphol 2001;9(4):297-301. > > On another note, the recommendation now is to not do CK IHC on sentinel lymph nodes (except for rare cases). Lymph node involvement should be based on H&E; not IHC. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy > Sent: Thursday, July 10, 2014 12:08 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Cytokeratin AE1/AE3 > > Hi Histonetters, > > I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". > We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. > I was wondering if anyone else has come across this, and if they have fixed it? > > Thank you, > > Brandy Burnett, HTL > Cape Cod Hospital > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk@CapeCodHealth.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Fri, 11 Jul 2014 12:41:04 -0400 > From: Ronda Mire > Subject: [Histonet] formalin/xylene monitoring > To: "" > > Message-ID: <8ECF026F-6B02-4483-8D66-8CEF31E5A323@cvpath.org> > Content-Type: text/plain; charset=us-ascii > > > Hello Netters > > I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. > > Ronda Mire > CVPath Institute > Gaithersburg, MD > > > ------------------------------ > > Message: 5 > Date: Fri, 11 Jul 2014 16:49:10 +0000 > From: Vincent Rivera > Subject: RE: [Histonet] formalin/xylene monitoring > To: 'Ronda Mire' , > "" > > Message-ID: > <3D4A471B82E7A44C87F6839732320D9F013F32C582@VSPDMS-ITEXMB02.DMS.COM> > Content-Type: text/plain; charset="us-ascii" > > Hi Ronda, > > It is located in the Anatomic Pathology Checklist safety section, I believe. > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire > Sent: Friday, July 11, 2014 9:41 AM > To: > Subject: [Histonet] formalin/xylene monitoring > > > Hello Netters > > I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. > > Ronda Mire > CVPath Institute > Gaithersburg, MD > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Fri, 11 Jul 2014 12:57:09 -0400 > From: Ronda Mire > Subject: Re: [Histonet] formalin/xylene monitoring > To: Vincent Rivera > Cc: "" > > Message-ID: <87801E1B-1FAD-46CB-908C-F97C257E18A1@cvpath.org> > Content-Type: text/plain; charset=us-ascii > > Found it, Thanks !!! >> On Jul 11, 2014, at 12:49 PM, Vincent Rivera wrote: >> >> Hi Ronda, >> >> It is located in the Anatomic Pathology Checklist safety section, I believe. >> >> Vincent Rivera, HT (ASCP), QIHC, QLS >> Histopathology Supervisor >> West Dermatology Pathology Laboratory >> vrivera@westderm.com >> 714-924-7240 (Lab) >> 714-390-0906 (Cell) >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire >> Sent: Friday, July 11, 2014 9:41 AM >> To: >> Subject: [Histonet] formalin/xylene monitoring >> >> >> Hello Netters >> >> I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. >> >> Ronda Mire >> CVPath Institute >> Gaithersburg, MD >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 128, Issue 10 > ***************************************** From HornHV <@t> archildrens.org Fri Jul 11 12:51:36 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jul 11 12:51:44 2014 Subject: [Histonet] Safety shower In-Reply-To: References: <53c01826.e762b60a.6173.3745SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <25A4DE08332B19499904459F00AAACB719DE6DEF0B@EVS1.archildrens.org> Our Maintenance department tests our showers each month. We test our eyewash stations each week. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alicia Lange Sent: Friday, July 11, 2014 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety shower I test our shower and eye wash weekly. We had a consulting company prepare us for an OSHA inspection and we were told then the testing needed to be done weekly for both. The temperate, flow, and clarity aren't necessarily tested just function. I use a 35 gallon trash bin to collect the water from the shower. I only run the shower enough to flush about 5 gallons out. Hope this helps! Alicia Lange HTL (ASCP) > On Jul 11, 2014, at 10:00 AM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: safety shower (Amber McKenzie) > 2. Job Opportunity (Baldwin, Kathy) > 3. RE: Cytokeratin AE1/AE3 (Cartun, Richard) > 4. formalin/xylene monitoring (Ronda Mire) > 5. RE: formalin/xylene monitoring (Vincent Rivera) > 6. Re: formalin/xylene monitoring (Ronda Mire) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 11 Jul 2014 14:36:14 +0000 > From: Amber McKenzie > Subject: [Histonet] RE: safety shower > To: John Shelley , Nancy Schmitt > , "histonet@lists.utsouthwestern.edu" > > Message-ID: <5A33C952BB67F4468AF1F36D739212BC011250E32A@JERRY.Gia.com> > Content-Type: text/plain; charset="iso-8859-1" > > What should the water volume be? Temperature and clarity? How do you test these things? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John > Shelley > Sent: Wednesday, July 09, 2014 12:39 PM > To: Nancy Schmitt; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: safety shower > > Our safety group here had built a neat little contraption using flexible PVC piping and making it circular, slightly larger than the shower head and placed a shower curtain and rings on it. When they pull the lever and use their system the water falls into a garbage can for collection. They come around once a month to check it out. > > Kind Regards! > > John J Shelley > Research Specialist, Histology Core Facility > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy > Schmitt > Sent: Wednesday, July 09, 2014 1:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] safety shower > > How often are you testing your safety shower ? Are you always checking for water volume, temperature and clarity? > > I appreciate your thoughts., > > Nancy Schmitt MLT, HT(ASCP) > Dubuque, IA > > > > NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Fri, 11 Jul 2014 14:49:35 +0000 > From: "Baldwin, Kathy" > Subject: [Histonet] Job Opportunity > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: <9e3d7cb10c43496c8e91f4e30af8a1bf@exch02.mhhcc.org> > Content-Type: text/plain; charset="us-ascii" > > Histology Tech position: Full time Monday thru Friday. Jasper, Indiana 47546Jasper is a regional center in southwestern Indiana, noted for its German Catholic ancestral roots Jasper has often been called the "Wood Capital of the World", boasting a large number of furniture companies, including Kimball International and Masterbrand Cabinets. Jasper is also home to the Southern Indiana Education Service Center (SIEC), Jasper Engines & Transmissions (largest remanufacturer in the market), and to a satellite campus of Vincennes University. > > JOB SUMMARY: > > Responsible for embedding, sectioning and cover slipping paraffin blocks and staining tissues to provide the pathologist with accurately prepared specimens for review and diagnosis. The employee works with specimens from patients of all ages. The employee must reflect mission statement and philosophy of Memorial Hospital and Health Care Center in daily work habits and contacts. > QUALIFICATIONS: > > Education: High school diploma or equivalent is required. Associate degree preferred. HT (ASCP) certification is preferred. Three years' experience as histology assist is acceptable. > > Training: Six months on the job training required. > > Experience: Previous histology experience is preferred. New graduates of histology school will be considered. > > S Kathy Baldwin > Histology/Cytology Supervisor > PH. 812-996-0210, Fax 812-996-0232 > sbaldwin@mhhcc.org > > "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" > > > > ------------------------------ > > Message: 3 > Date: Fri, 11 Jul 2014 16:08:56 +0000 > From: "Cartun, Richard" > Subject: [Histonet] RE: Cytokeratin AE1/AE3 > To: "Rathborne, Toni" , "Burnett, Brandy" > , "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > <9215BD4B0BA1B44D962A71C758B68D2E355B5979@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="us-ascii" > > Reference: Weaver DL, et al.: Effect of occult metastases on survival in node-negative breast cancer. NEJM 2011;364:412-421. A study funded by the National Cancer Institute (Clinical Trials). > > Conclusion - "These data do not indicate a clinical benefit of additional evaluation, including immunohistochemical analysis, of initially negative sentinel nodes in patients with breast cancer. > > Also, from an NSABP study: Role of IHC in invasive lobular carcinoma - Although routine IHC staining with cytokeratin is not indicated for most breast cancers, it can be helpful for examination of the sentinel nodes in patients with invasive lobular carcinoma since the morphology of lobular cancer can be difficult to detect on H&E of axillary lymph nodes [91,92]. In general, IHC should be used to definitively diagnose an area that is suspicious for, but not diagnostic of, lymph node metastases on H&E rather than as a routine method of evaluating nodes in cases of invasive lobular cancer. > > I believe most major cancer centers have stopped doing cytokeratin IHC on sentinel nodes for most breast cancer specimens. I know that we have. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: Rathborne, Toni [mailto:Toni.Rathborne@rwjuh.edu] > Sent: Thursday, July 10, 2014 4:49 PM > To: Cartun, Richard; Burnett, Brandy; 'histonet@lists.utsouthwestern.edu' > Subject: RE: Cytokeratin AE1/AE3 > > Would you share the source for the sentinel lymph node recommendation mentioned? Although I just did a quick search, I didn't see anything on the CAP website. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Cartun, Richard > Sent: Thursday, July 10, 2014 4:08 PM > To: Burnett, Brandy; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Cytokeratin AE1/AE3 > > "Extra-follicular reticulm cells" contain cytokeratin and will label with antibodies to low molecular weight cytokeratin. Could that be what you are seeing? > > Reference: Linden MD and Zarbo RJ: Cytokeratin immunostaining patterns of benign, reactive lymph nodes: applications for the evaluation of sentinel lymph node specimen. Appl Immunohistochem Mol Morphol 2001;9(4):297-301. > > On another note, the recommendation now is to not do CK IHC on sentinel lymph nodes (except for rare cases). Lymph node involvement should be based on H&E; not IHC. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Burnett, Brandy > Sent: Thursday, July 10, 2014 12:08 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Cytokeratin AE1/AE3 > > Hi Histonetters, > > I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". > We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. > I was wondering if anyone else has come across this, and if they have fixed it? > > Thank you, > > Brandy Burnett, HTL > Cape Cod Hospital > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk@CapeCodHealth.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Fri, 11 Jul 2014 12:41:04 -0400 > From: Ronda Mire > Subject: [Histonet] formalin/xylene monitoring > To: "" > > Message-ID: <8ECF026F-6B02-4483-8D66-8CEF31E5A323@cvpath.org> > Content-Type: text/plain; charset=us-ascii > > > Hello Netters > > I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. > > Ronda Mire > CVPath Institute > Gaithersburg, MD > > > ------------------------------ > > Message: 5 > Date: Fri, 11 Jul 2014 16:49:10 +0000 > From: Vincent Rivera > Subject: RE: [Histonet] formalin/xylene monitoring > To: 'Ronda Mire' , > "" > > Message-ID: > > <3D4A471B82E7A44C87F6839732320D9F013F32C582@VSPDMS-ITEXMB02.DMS.COM> > Content-Type: text/plain; charset="us-ascii" > > Hi Ronda, > > It is located in the Anatomic Pathology Checklist safety section, I believe. > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda > Mire > Sent: Friday, July 11, 2014 9:41 AM > To: > Subject: [Histonet] formalin/xylene monitoring > > > Hello Netters > > I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. > > Ronda Mire > CVPath Institute > Gaithersburg, MD > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Fri, 11 Jul 2014 12:57:09 -0400 > From: Ronda Mire > Subject: Re: [Histonet] formalin/xylene monitoring > To: Vincent Rivera > Cc: "" > > Message-ID: <87801E1B-1FAD-46CB-908C-F97C257E18A1@cvpath.org> > Content-Type: text/plain; charset=us-ascii > > Found it, Thanks !!! >> On Jul 11, 2014, at 12:49 PM, Vincent Rivera wrote: >> >> Hi Ronda, >> >> It is located in the Anatomic Pathology Checklist safety section, I believe. >> >> Vincent Rivera, HT (ASCP), QIHC, QLS >> Histopathology Supervisor >> West Dermatology Pathology Laboratory vrivera@westderm.com >> 714-924-7240 (Lab) >> 714-390-0906 (Cell) >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda >> Mire >> Sent: Friday, July 11, 2014 9:41 AM >> To: >> Subject: [Histonet] formalin/xylene monitoring >> >> >> Hello Netters >> >> I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. >> >> Ronda Mire >> CVPath Institute >> Gaithersburg, MD >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 128, Issue 10 > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Rebecca.Riesen <@t> hma.com Fri Jul 11 13:08:27 2014 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Fri Jul 11 13:09:06 2014 Subject: [Histonet] PA's Message-ID: I would like to get an idea of how many hospitals that contract in the Pathologist group to read slides produced by hospital employed Histotechs, have that Pathologist group paying the salary of their PA's. I have heard that many facilities are no longer employing the PA's that actually are performing the Pathologist's job of gross dissection (hence the name Pathologist Assistant, huh). In these tough financial times we all are looking for ways to lessen our costs. You can reply to me directly rather than clog up the histonet. THANKS!! From Joyce.Weems <@t> emoryhealthcare.org Fri Jul 11 13:19:13 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jul 11 13:19:34 2014 Subject: [Histonet] Safety shower In-Reply-To: <25A4DE08332B19499904459F00AAACB719DE6DEF0B@EVS1.archildrens.org> References: <53c01826.e762b60a.6173.3745SMTPIN_ADDED_MISSING@mx.google.com> <25A4DE08332B19499904459F00AAACB719DE6DEF0B@EVS1.archildrens.org> Message-ID: Same here Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, July 11, 2014 1:52 PM To: 'Alicia Lange'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety shower Our Maintenance department tests our showers each month. We test our eyewash stations each week. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alicia Lange Sent: Friday, July 11, 2014 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety shower I test our shower and eye wash weekly. We had a consulting company prepare us for an OSHA inspection and we were told then the testing needed to be done weekly for both. The temperate, flow, and clarity aren't necessarily tested just function. I use a 35 gallon trash bin to collect the water from the shower. I only run the shower enough to flush about 5 gallons out. Hope this helps! Alicia Lange HTL (ASCP) > On Jul 11, 2014, at 10:00 AM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: safety shower (Amber McKenzie) > 2. Job Opportunity (Baldwin, Kathy) > 3. RE: Cytokeratin AE1/AE3 (Cartun, Richard) > 4. formalin/xylene monitoring (Ronda Mire) > 5. RE: formalin/xylene monitoring (Vincent Rivera) > 6. Re: formalin/xylene monitoring (Ronda Mire) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 11 Jul 2014 14:36:14 +0000 > From: Amber McKenzie > Subject: [Histonet] RE: safety shower > To: John Shelley , Nancy Schmitt > , "histonet@lists.utsouthwestern.edu" > > Message-ID: <5A33C952BB67F4468AF1F36D739212BC011250E32A@JERRY.Gia.com> > Content-Type: text/plain; charset="iso-8859-1" > > What should the water volume be? Temperature and clarity? How do you test these things? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John > Shelley > Sent: Wednesday, July 09, 2014 12:39 PM > To: Nancy Schmitt; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: safety shower > > Our safety group here had built a neat little contraption using flexible PVC piping and making it circular, slightly larger than the shower head and placed a shower curtain and rings on it. When they pull the lever and use their system the water falls into a garbage can for collection. They come around once a month to check it out. > > Kind Regards! > > John J Shelley > Research Specialist, Histology Core Facility > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy > Schmitt > Sent: Wednesday, July 09, 2014 1:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] safety shower > > How often are you testing your safety shower ? Are you always checking for water volume, temperature and clarity? > > I appreciate your thoughts., > > Nancy Schmitt MLT, HT(ASCP) > Dubuque, IA > > > > NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Fri, 11 Jul 2014 14:49:35 +0000 > From: "Baldwin, Kathy" > Subject: [Histonet] Job Opportunity > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: <9e3d7cb10c43496c8e91f4e30af8a1bf@exch02.mhhcc.org> > Content-Type: text/plain; charset="us-ascii" > > Histology Tech position: Full time Monday thru Friday. Jasper, Indiana 47546Jasper is a regional center in southwestern Indiana, noted for its German Catholic ancestral roots Jasper has often been called the "Wood Capital of the World", boasting a large number of furniture companies, including Kimball International and Masterbrand Cabinets. Jasper is also home to the Southern Indiana Education Service Center (SIEC), Jasper Engines & Transmissions (largest remanufacturer in the market), and to a satellite campus of Vincennes University. > > JOB SUMMARY: > > Responsible for embedding, sectioning and cover slipping paraffin blocks and staining tissues to provide the pathologist with accurately prepared specimens for review and diagnosis. The employee works with specimens from patients of all ages. The employee must reflect mission statement and philosophy of Memorial Hospital and Health Care Center in daily work habits and contacts. > QUALIFICATIONS: > > Education: High school diploma or equivalent is required. Associate degree preferred. HT (ASCP) certification is preferred. Three years' experience as histology assist is acceptable. > > Training: Six months on the job training required. > > Experience: Previous histology experience is preferred. New graduates of histology school will be considered. > > S Kathy Baldwin > Histology/Cytology Supervisor > PH. 812-996-0210, Fax 812-996-0232 > sbaldwin@mhhcc.org > > "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" > > > > ------------------------------ > > Message: 3 > Date: Fri, 11 Jul 2014 16:08:56 +0000 > From: "Cartun, Richard" > Subject: [Histonet] RE: Cytokeratin AE1/AE3 > To: "Rathborne, Toni" , "Burnett, Brandy" > , "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > <9215BD4B0BA1B44D962A71C758B68D2E355B5979@HHCEXCHMB05.hhcsystem.org> > Content-Type: text/plain; charset="us-ascii" > > Reference: Weaver DL, et al.: Effect of occult metastases on survival in node-negative breast cancer. NEJM 2011;364:412-421. A study funded by the National Cancer Institute (Clinical Trials). > > Conclusion - "These data do not indicate a clinical benefit of additional evaluation, including immunohistochemical analysis, of initially negative sentinel nodes in patients with breast cancer. > > Also, from an NSABP study: Role of IHC in invasive lobular carcinoma - Although routine IHC staining with cytokeratin is not indicated for most breast cancers, it can be helpful for examination of the sentinel nodes in patients with invasive lobular carcinoma since the morphology of lobular cancer can be difficult to detect on H&E of axillary lymph nodes [91,92]. In general, IHC should be used to definitively diagnose an area that is suspicious for, but not diagnostic of, lymph node metastases on H&E rather than as a routine method of evaluating nodes in cases of invasive lobular cancer. > > I believe most major cancer centers have stopped doing cytokeratin IHC on sentinel nodes for most breast cancer specimens. I know that we have. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: Rathborne, Toni [mailto:Toni.Rathborne@rwjuh.edu] > Sent: Thursday, July 10, 2014 4:49 PM > To: Cartun, Richard; Burnett, Brandy; 'histonet@lists.utsouthwestern.edu' > Subject: RE: Cytokeratin AE1/AE3 > > Would you share the source for the sentinel lymph node recommendation mentioned? Although I just did a quick search, I didn't see anything on the CAP website. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Cartun, Richard > Sent: Thursday, July 10, 2014 4:08 PM > To: Burnett, Brandy; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Cytokeratin AE1/AE3 > > "Extra-follicular reticulm cells" contain cytokeratin and will label with antibodies to low molecular weight cytokeratin. Could that be what you are seeing? > > Reference: Linden MD and Zarbo RJ: Cytokeratin immunostaining patterns of benign, reactive lymph nodes: applications for the evaluation of sentinel lymph node specimen. Appl Immunohistochem Mol Morphol 2001;9(4):297-301. > > On another note, the recommendation now is to not do CK IHC on sentinel lymph nodes (except for rare cases). Lymph node involvement should be based on H&E; not IHC. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Burnett, Brandy > Sent: Thursday, July 10, 2014 12:08 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Cytokeratin AE1/AE3 > > Hi Histonetters, > > I just came across a thread regarding issues with Cytokeratin AE1/AE3 from DAKO. I am having the same issues with our New protocol using HIER on the Link-48 in which sentinel lymph nodes from breast cases are staining non-specifically "in-between the cells". > We never had this issue before on our old Autostainer plus, however, we were using only enzyme pre-treatment on it before staining. > I was wondering if anyone else has come across this, and if they have fixed it? > > Thank you, > > Brandy Burnett, HTL > Cape Cod Hospital > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk@CapeCodHealth.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Fri, 11 Jul 2014 12:41:04 -0400 > From: Ronda Mire > Subject: [Histonet] formalin/xylene monitoring > To: "" > > Message-ID: <8ECF026F-6B02-4483-8D66-8CEF31E5A323@cvpath.org> > Content-Type: text/plain; charset=us-ascii > > > Hello Netters > > I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. > > Ronda Mire > CVPath Institute > Gaithersburg, MD > > > ------------------------------ > > Message: 5 > Date: Fri, 11 Jul 2014 16:49:10 +0000 > From: Vincent Rivera > Subject: RE: [Histonet] formalin/xylene monitoring > To: 'Ronda Mire' , > "" > > Message-ID: > > <3D4A471B82E7A44C87F6839732320D9F013F32C582@VSPDMS-ITEXMB02.DMS.COM> > Content-Type: text/plain; charset="us-ascii" > > Hi Ronda, > > It is located in the Anatomic Pathology Checklist safety section, I believe. > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda > Mire > Sent: Friday, July 11, 2014 9:41 AM > To: > Subject: [Histonet] formalin/xylene monitoring > > > Hello Netters > > I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. > > Ronda Mire > CVPath Institute > Gaithersburg, MD > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Fri, 11 Jul 2014 12:57:09 -0400 > From: Ronda Mire > Subject: Re: [Histonet] formalin/xylene monitoring > To: Vincent Rivera > Cc: "" > > Message-ID: <87801E1B-1FAD-46CB-908C-F97C257E18A1@cvpath.org> > Content-Type: text/plain; charset=us-ascii > > Found it, Thanks !!! >> On Jul 11, 2014, at 12:49 PM, Vincent Rivera wrote: >> >> Hi Ronda, >> >> It is located in the Anatomic Pathology Checklist safety section, I believe. >> >> Vincent Rivera, HT (ASCP), QIHC, QLS >> Histopathology Supervisor >> West Dermatology Pathology Laboratory vrivera@westderm.com >> 714-924-7240 (Lab) >> 714-390-0906 (Cell) >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda >> Mire >> Sent: Friday, July 11, 2014 9:41 AM >> To: >> Subject: [Histonet] formalin/xylene monitoring >> >> >> Hello Netters >> >> I just received my CAP self inspection checklist and I do not see the question regarding Xylene/Formaldehyde monitoring. Has this checklist item been removed? Is monitoring no longer necessary? What gives? I have checked the CAP website but haven't found anything. >> >> Ronda Mire >> CVPath Institute >> Gaithersburg, MD >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 128, Issue 10 > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Jul 11 13:19:01 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jul 11 13:19:48 2014 Subject: [Histonet] RE: PA's In-Reply-To: References: Message-ID: Our group hired their PA. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Friday, July 11, 2014 2:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PA's I would like to get an idea of how many hospitals that contract in the Pathologist group to read slides produced by hospital employed Histotechs, have that Pathologist group paying the salary of their PA's. I have heard that many facilities are no longer employing the PA's that actually are performing the Pathologist's job of gross dissection (hence the name Pathologist Assistant, huh). In these tough financial times we all are looking for ways to lessen our costs. You can reply to me directly rather than clog up the histonet. THANKS!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From tbraud <@t> holyredeemer.com Fri Jul 11 13:35:51 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Jul 11 13:36:03 2014 Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 10 In-Reply-To: <20140711161134.F09ED1E807C@trendmess-svr.holyredeemer.local> References: <20140711161134.F09ED1E807C@trendmess-svr.holyredeemer.local> Message-ID: Yep, we're with you, Richard. We've stopped doing cytokeratins on sentinel node in breast ca cases, except lobular ca cases. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 3. RE: Cytokeratin AE1/AE3 (Cartun, Richard) Message: 3 Date: Fri, 11 Jul 2014 16:08:56 +0000 From: "Cartun, Richard" Subject: [Histonet] RE: Cytokeratin AE1/AE3 Reference: Weaver DL, et al.: Effect of occult metastases on survival in node-negative breast cancer. NEJM 2011;364:412-421. A study funded by the National Cancer Institute (Clinical Trials). Conclusion - "These data do not indicate a clinical benefit of additional evaluation, including immunohistochemical analysis, of initially negative sentinel nodes in patients with breast cancer. Also, from an NSABP study: Role of IHC in invasive lobular carcinoma - Although routine IHC staining with cytokeratin is not indicated for most breast cancers, it can be helpful for examination of the sentinel nodes in patients with invasive lobular carcinoma since the morphology of lobular cancer can be difficult to detect on H&E of axillary lymph nodes [91,92]. In general, IHC should be used to definitively diagnose an area that is suspicious for, but not diagnostic of, lymph node metastases on H&E rather than as a routine method of evaluating nodes in cases of invasive lobular cancer. I believe most major cancer centers have stopped doing cytokeratin IHC on sentinel nodes for most breast cancer specimens. I know that we have. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From jerrysedgewick <@t> gmail.com Fri Jul 11 14:26:21 2014 From: jerrysedgewick <@t> gmail.com (jerry sedgewick) Date: Fri Jul 11 14:26:34 2014 Subject: [Histonet] Wright-Geimsa Stain Message-ID: <53C03A5D.1030600@gmail.com> I'm doing a project where I'm measuring color differences from histology session to histology session. Not being a histologist, I'm not certain whether RBCs can be used as a feature to measure for color change with Wright-Giemsa. I suppose it depends on the Eosin Y concentration, but, even at low concentrations, should I see RBCs stained? Thanks! Jerry Sedgewick From rperez <@t> medsurgpath.com Fri Jul 11 19:24:36 2014 From: rperez <@t> medsurgpath.com (Rolly Perez) Date: Fri Jul 11 19:25:27 2014 Subject: [Histonet] Tissue Tek Prisma Model 6130 Message-ID: <6A95511DF5508E44AED8151EF588B4AF011750@W14MBX-CH2-A4.w14a.comcast.net> Any experience-based resolution on the problem of the touch screen pad/monitor of the Tissue Tek Prisma Stainer 6130 wherein the screen turns black while a program is running. The program continues to run even though the screen is not illuminated. I thought I'd throw this out there in case someone knows a way to resolve it before I call for repair. I'm still able to use the stainer by placing sticker labels on the screen so I know where to press :-) Rolly Perez Histology Supervisor | MedSurg Pathology 8192 SW Durham Road, Tigard OR 97224 Tel: 503.443.2157 | Fax: 503.670.9754 From rjbuesa <@t> yahoo.com Sat Jul 12 09:16:24 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 12 09:16:31 2014 Subject: [Histonet] Wright-Geimsa Stain In-Reply-To: <53C03A5D.1030600@gmail.com> References: <53C03A5D.1030600@gmail.com> Message-ID: <1405174584.25193.YahooMailNeo@web120401.mail.ne1.yahoo.com> Are you using smears or tissue sections? Imagining you are using the former; eosin color in RBC should vary according with: 1- "age" of the RBC (after losing their nucleus); 2- staining technique (time, fixation and "age" of the solution); 3- type and concentration of eosin. So I think that you first should create a standard technique (including the one to measure "color intensity") and latter try your idea. For me it is very interesting why do you want to do this? Ren? J. On Friday, July 11, 2014 3:26 PM, jerry sedgewick wrote: I'm doing a project where I'm measuring color differences from histology session to histology session.? Not being a histologist, I'm not certain whether RBCs can be used as a feature to measure for color change with Wright-Giemsa.? I suppose it depends on the Eosin Y concentration, but, even at low concentrations, should I see RBCs stained? Thanks! Jerry Sedgewick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Jul 12 09:19:05 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 12 09:19:12 2014 Subject: [Histonet] PA's In-Reply-To: References: Message-ID: <1405174745.34677.YahooMailNeo@web120406.mail.ne1.yahoo.com> Being a teaching hospital,?the pathology residents (during their rotation) take care of gross descriptions and autopsies. No need for PAs. Ren? J.? On Friday, July 11, 2014 2:09 PM, "Riesen, Rebecca" wrote: I would like to get an idea of how many hospitals that contract in the Pathologist group to read slides produced by hospital employed Histotechs, have that Pathologist group paying the salary of their PA's.? I have heard that many facilities are no longer employing the PA's that actually are performing the Pathologist's job of gross dissection (hence the name Pathologist Assistant, huh).? In these tough financial times we all are looking for ways to lessen our costs.? You can reply to me directly rather than clog up the histonet.? THANKS!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Jul 12 14:17:48 2014 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Sat Jul 12 14:18:01 2014 Subject: [Histonet] Image uploading Message-ID: <1405192652599.19189@kcl.ac.uk> I make mistakes when I upload images.... So, I'd be most grateful for an edit function to be included. Thanks for the opportunity to share images. Sure, the excellent Immunoportal was The Best...I hope that Histonet can equal that website quality? Carlos Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From kennthom1 <@t> centurylink.net Sat Jul 12 16:14:18 2014 From: kennthom1 <@t> centurylink.net (Carolyn Thompson) Date: Sat Jul 12 16:14:25 2014 Subject: [Histonet] please remove me Message-ID: <000001cf9e16$3f1a96d0$bd4fc470$@centurylink.net> Carol Thompson From Joyce.Weems <@t> emoryhealthcare.org Mon Jul 14 07:42:54 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Jul 14 07:43:08 2014 Subject: [Histonet] please remove me In-Reply-To: <000001cf9e16$3f1a96d0$bd4fc470$@centurylink.net> References: <000001cf9e16$3f1a96d0$bd4fc470$@centurylink.net> Message-ID: Hi Carolyn, You must go to this website http://lists.utsouthwestern.edu/mailman/listinfo/histonet and do this yourself. Best, Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carolyn Thompson Sent: Saturday, July 12, 2014 5:14 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please remove me Carol Thompson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From LSebree <@t> uwhealth.org Mon Jul 14 07:44:17 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Jul 14 07:44:24 2014 Subject: [Histonet] Storage of HER2 neu control slides Message-ID: <77DD817201982748BC67D7960F2F76AF0BC322@UWHC-MBX12.uwhis.hosp.wisc.edu> Good morning, I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? Thanks for any and all replies, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From KSimeone <@t> leavittmgt.com Mon Jul 14 08:30:56 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon Jul 14 08:31:22 2014 Subject: [Histonet] FT DERM POSITION DELRAY BEACH, FLORIDA In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C2FD9FC68@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C2FD9FC68@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C2FDA00FF@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Derm Lab. This is a permanent full time position with benefits (medical/401k/vacation). ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann@leavittmgt.com if interested. *full time position Mon-Fri 11a-7:30p (hours NON NEGOTIABLE) *MUST be licensed as a FL histotechnologist or technician *MUST have at least 2 years experience (dermatology preferred) *VERY proficient in embedding and microtomy *experience in grossing and immunohistochemistry (BOND) a plus *must be self motivated and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Richard.Cartun <@t> hhchealth.org Mon Jul 14 09:22:43 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Jul 14 09:22:50 2014 Subject: [Histonet] RE: Storage of HER2 neu control slides In-Reply-To: <77DD817201982748BC67D7960F2F76AF0BC322@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF0BC322@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355B8BF6@HHCEXCHMB03.hhcsystem.org> We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, July 14, 2014 8:44 AM To: Histonet (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] Storage of HER2 neu control slides Good morning, I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? Thanks for any and all replies, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From joelleweaver <@t> hotmail.com Mon Jul 14 09:25:13 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Mon Jul 14 09:25:22 2014 Subject: [Histonet] RE: Storage of HER2 neu control slides In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355B8BF6@HHCEXCHMB03.hhcsystem.org> References: <77DD817201982748BC67D7960F2F76AF0BC322@UWHC-MBX12.uwhis.hosp.wisc.edu>, <9215BD4B0BA1B44D962A71C758B68D2E355B8BF6@HHCEXCHMB03.hhcsystem.org> Message-ID: I do the complete drain & dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Richard.Cartun@hhchealth.org > To: LSebree@uwhealth.org; Histonet@lists.utsouthwestern.edu > Date: Mon, 14 Jul 2014 14:22:43 +0000 > CC: > Subject: [Histonet] RE: Storage of HER2 neu control slides > > We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A > Sent: Monday, July 14, 2014 8:44 AM > To: Histonet (Histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Storage of HER2 neu control slides > > Good morning, > > I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? > > Thanks for any and all replies, > > Linda > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From huimin.wang <@t> sickkids.ca Mon Jul 14 09:39:18 2014 From: huimin.wang <@t> sickkids.ca (Huimin Wang) Date: Mon Jul 14 09:39:39 2014 Subject: [Histonet] please remove me Message-ID: <3406E4136F0871428A3491FE6642ED88790A57B5@SKMBXX02.sickkids.ca> please remove me, thanks ________________________________ This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. From Lisa.Lavigne <@t> sphp.com Mon Jul 14 09:46:52 2014 From: Lisa.Lavigne <@t> sphp.com (Lavigne, Lisa) Date: Mon Jul 14 09:47:26 2014 Subject: [Histonet] RE: please remove me In-Reply-To: <3406E4136F0871428A3491FE6642ED88790A57B5@SKMBXX02.sickkids.ca> References: <3406E4136F0871428A3491FE6642ED88790A57B5@SKMBXX02.sickkids.ca> Message-ID: <58B7B6DE8E5609439675A7C9FE521A9AFA3C24@CHEXCMS13.one.ads.che.org> Please remove me, thanks Lisa LaVigne CT, MB(ASCP) Pathology Manager, St. Peter's Health Partners 315 S. Manning Blvd. Albany, NY 12208 518-525-5274 Lisa.Lavigne@sphp.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Huimin Wang Sent: Monday, July 14, 2014 10:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please remove me please remove me, thanks ________________________________ This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From LSebree <@t> uwhealth.org Mon Jul 14 09:59:41 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Mon Jul 14 09:59:59 2014 Subject: [Histonet] RE: Storage of HER2 neu control slides In-Reply-To: References: <77DD817201982748BC67D7960F2F76AF0BC322@UWHC-MBX12.uwhis.hosp.wisc.edu>, <9215BD4B0BA1B44D962A71C758B68D2E355B8BF6@HHCEXCHMB03.hhcsystem.org> Message-ID: <77DD817201982748BC67D7960F2F76AF0BC3F2@UWHC-MBX12.uwhis.hosp.wisc.edu> Thanks Joelle. I'm surprised that Oracle is suggesting overnight baking as I noticed a huge difference when I dried some test tissue controls for 30" @ 60 degrees vs 10" @ 60 degrees. I was testing some blocks that I had 3 cores of varying degrees of intensity. All showed much decreased reactivity at 30" vs 10". In fact, the 2 weaker cores were virtually negative. I can only assume you're using a different clone than we are and that there is different epitope stability between clones. Thanks for your response, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From: Joelle Weaver [mailto:joelleweaver@hotmail.com] Sent: Monday, July 14, 2014 9:25 AM To: Cartun, Richard; Sebree Linda A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Storage of HER2 neu control slides I do the complete drain & dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Richard.Cartun@hhchealth.org > To: LSebree@uwhealth.org; Histonet@lists.utsouthwestern.edu > Date: Mon, 14 Jul 2014 14:22:43 +0000 > CC: > Subject: [Histonet] RE: Storage of HER2 neu control slides > > We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A > Sent: Monday, July 14, 2014 8:44 AM > To: Histonet (Histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Storage of HER2 neu control slides > > Good morning, > > I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? > > Thanks for any and all replies, > > Linda > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mw <@t> personifysearch.com Mon Jul 14 11:03:02 2014 From: mw <@t> personifysearch.com (Matt Ward) Date: Mon Jul 14 11:03:09 2014 Subject: [Histonet] New Histology Opportunity - Chicago Message-ID: <01ed01cf9f7d$182e0030$488a0090$@personifysearch.com> Good morning! We have had a top company open a new Field Histology Specialist to cover the Chicago and Midwest Region. The company is a world leading histology manufacturer and this opportunity is perfect for a histology professional who is looking to break out of the laboratory and into the field. The position offers a competitive salary + bonus, company car, paid expenses, and full benefits. If you or someone you may know would be interested in learning more, please contact me directly at mw@personifysearch.com. Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com From joelleweaver <@t> hotmail.com Mon Jul 14 12:27:19 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Mon Jul 14 12:27:27 2014 Subject: [Histonet] RE: Storage of HER2 neu control slides In-Reply-To: <77DD817201982748BC67D7960F2F76AF0BC3F2@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF0BC322@UWHC-MBX12.uwhis.hosp.wisc.edu>, <9215BD4B0BA1B44D962A71C758B68D2E355B8BF6@HHCEXCHMB03.hhcsystem.org>, , <77DD817201982748BC67D7960F2F76AF0BC3F2@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Just started using it ( currently doing validation & correlation), so maybe will be issues at some later time. I just noticed the "overnight" in the IFU file from their website when I was putting the SOP together. I have to be honest, that I did my standard preparation so far and it worked great, and had no issues with fall off or staining. Since it is IVD, was considering in my mind, if shorter baking would be a "modification"? The kit I have uses CB11. Joelle Weaver MAOM, HTL (ASCP) QIHC From: LSebree@uwhealth.org To: joelleweaver@hotmail.com CC: histonet@lists.utsouthwestern.edu; Richard.Cartun@hhchealth.org Subject: RE: [Histonet] RE: Storage of HER2 neu control slides Date: Mon, 14 Jul 2014 14:59:41 +0000 Thanks Joelle. I?m surprised that Oracle is suggesting overnight baking as I noticed a huge difference when I dried some test tissue controls for 30? @ 60 degrees vs 10? @ 60 degrees. I was testing some blocks that I had 3 cores of varying degrees of intensity. All showed much decreased reactivity at 30? vs 10?. In fact, the 2 weaker cores were virtually negative. I can only assume you?re using a different clone than we are and that there is different epitope stability between clones. Thanks for your response, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From: Joelle Weaver [mailto:joelleweaver@hotmail.com] Sent: Monday, July 14, 2014 9:25 AM To: Cartun, Richard; Sebree Linda A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Storage of HER2 neu control slides I do the complete drain & dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Richard.Cartun@hhchealth.org > To: LSebree@uwhealth.org; Histonet@lists.utsouthwestern.edu > Date: Mon, 14 Jul 2014 14:22:43 +0000 > CC: > Subject: [Histonet] RE: Storage of HER2 neu control slides > > We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A > Sent: Monday, July 14, 2014 8:44 AM > To: Histonet (Histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Storage of HER2 neu control slides > > Good morning, > > I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? > > Thanks for any and all replies, > > Linda > > > Linda A. Sebree > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lsgraves70 <@t> gmail.com Mon Jul 14 12:55:55 2014 From: lsgraves70 <@t> gmail.com (Leslie Graves) Date: Mon Jul 14 12:55:58 2014 Subject: [Histonet] IHC Validation Message-ID: Does anyone have a sample IHC Validation worksheet that they used in their lab for review. I know that the requirements have changed since our last inspection. I want to make sure that I am including all of the criteria, and would like to see how other labs are documenting this process. Leslie Graves Technical Specialist Cape Fear Valley Medical Center 1638 Owen Drive Fayetteville, NC 28341 910-615-6142 lgrav@capefearvalley.com From Vickroy.Jim <@t> mhsil.com Mon Jul 14 13:37:53 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Jul 14 13:38:05 2014 Subject: [Histonet] Bone Marrow Trephine Cases Message-ID: Over the past several years we have had some questions by our hematopathologists regarding the quality of the trephine blocks. The biggest concern has been that the sections seem to have a fragmented appearance which we have determined in the past to be areas where the lipid cell borders break. We have found that the artifact is diminished when we pick the sections up as quickly as we can which led us to believe that the cell borders break when they are left on the warm water in the waterbath. Some of the pathologists still believe the artifact is caused by the decalcification process. I have reviewed protocols before and obviously found that labs use different decalcifying agents from a harsh HCL solution to a weaker formic acid solution. We currently use Immunocal which I believe is a formic acid solution. I have also been introduced to some new instrumentation available such as the Milestone Bone Station which uses a EDTA solution with some added heat and agitation. I realize that EDTA yields more DNA and RNA which is obviously an advantage however the length of time for the decal process with EDTA is much longer. I'm not sure that using EDTA for decalcification will however reduce the artifact that the pathologists see (fragmentation and breaking up of the lipid cells in the sections. I would appreciate any information that others might be able to share from their experiences and if they have dealt with a similar problem. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 217-415-7505 Cell ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Richard.Cartun <@t> hhchealth.org Mon Jul 14 14:50:08 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Jul 14 14:50:13 2014 Subject: [Histonet] IHC for Adrenal hormones? Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355BB0A3@HHCEXCHMB03.hhcsystem.org> Is anyone doing IHC for adrenal corticosteroid and androgen hormones? We have an adrenal tumor in a 10 y.o. patient and the clinicians want to see what the tumor is producing. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From ttroyer <@t> petersonlab.com Mon Jul 14 15:35:58 2014 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Mon Jul 14 15:36:03 2014 Subject: [Histonet] Used Benchmark Message-ID: <001c01cf9fa3$38977790$a9c666b0$@petersonlab.com> I have a used Ventana Benchmark that is collecting dust in our storeroom. Would anyone be interested in this piece of equipment? I have pics and would be willing to answer any questions. When we put it in the store room it was in working order. Thanks, Travis From klaus.dern44 <@t> gmail.com Tue Jul 15 07:23:34 2014 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Tue Jul 15 07:23:38 2014 Subject: [Histonet] THICK AND THIN SECTIONS ? Message-ID: If you are using one of the following Microtomes and the advance mechanism is worn out. (too much play) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome, because these Models are no longer supported by the Manufacturer. ( no parts availability ) Rather than replacing these excellent instruments, I have a PERMANENT solution to this problem. For Information contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com From cjohnson <@t> nmda.nmsu.edu Tue Jul 15 07:31:43 2014 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Tue Jul 15 07:31:55 2014 Subject: [Histonet] IHC for plague and tularemia Message-ID: Hi everyone, I would love to hear from anyone who is running plague and/or tularemia IHC. If you have a protocol you are willing to share and/or vendor information it would be greatly appreciated. Thank you in advance! Carole Johnson, HT(ASCP)cm Histopathology Section Supervisor New Mexico Department of Agriculture Veterinary Diagnostic Services Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From abtdhu <@t> gmail.com Tue Jul 15 07:40:15 2014 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Tue Jul 15 07:40:44 2014 Subject: [Histonet] Can I use no water benzoyl peroxide from PolySciences in my MMA mixture? Message-ID: <720FB235-8DE1-4DAD-9D5A-432C3A2DEF81@gmail.com> I try to avoid CaCl drying step in making MMA infiltration and embedding solutions. Please help me MMA experts. Many lab use benzoyl peroxide which has 30% water in it. And go through CaCl afterwards. Another question, why some lab use Butanol and toluene before coverslip? Why use Cellosolve to do deplastify? I think xylene and ethanol are cheaper and less toxic, is it true? Thanks for you reply. Dorothy MGH endocrine histocore Sent from my iPad From lsgraves70 <@t> gmail.com Tue Jul 15 08:51:03 2014 From: lsgraves70 <@t> gmail.com (Leslie Graves) Date: Tue Jul 15 08:51:08 2014 Subject: [Histonet] Re: IHC Validation In-Reply-To: References: Message-ID: Thank you to everyone who sent a template. This is very helpful. I do need clarfication on the 10 cases that are required for validation. Do you include the 10 cases on this same form, or do you have something separate for that? These look ideal for optimization. From what I have read online, 10 cases need to be run after optimization has been completed. When validating these 10 cases, accuracy, sensitivity, specificity and reproducibility need to be monitored. Are all of you using separate form to capture this data and approval from the Pathologists? Maybe I am going overboard, but I want to make sure that I have everything covered. We are bringing a new platform into our lab in 2 weeks, and I want to have everything ready to go. Leslie Graves Technical Specialist Cape Fear Valley Medical Center Fayetteville, NC 28341 910-615-6142 lgrav@capefearvalley.com On Mon, Jul 14, 2014 at 1:55 PM, Leslie Graves wrote: > Does anyone have a sample IHC Validation worksheet that they used in their > lab for review. I know that the requirements have changed since our last > inspection. I want to make sure that I am including all of the criteria, > and would like to see how other labs are documenting this process. > > Leslie Graves > Technical Specialist > Cape Fear Valley Medical Center > 1638 Owen Drive > Fayetteville, NC 28341 > 910-615-6142 > lgrav@capefearvalley.com > From amber.mckenzie <@t> gastrodocs.net Tue Jul 15 09:05:53 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jul 15 09:06:08 2014 Subject: [Histonet] IHC Validation In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC011250F160@JERRY.Gia.com> Can you forward any templates to me as well? Thanks! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leslie Graves Sent: Tuesday, July 15, 2014 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: IHC Validation Thank you to everyone who sent a template. This is very helpful. I do need clarfication on the 10 cases that are required for validation. Do you include the 10 cases on this same form, or do you have something separate for that? These look ideal for optimization. From what I have read online, 10 cases need to be run after optimization has been completed. When validating these 10 cases, accuracy, sensitivity, specificity and reproducibility need to be monitored. Are all of you using separate form to capture this data and approval from the Pathologists? Maybe I am going overboard, but I want to make sure that I have everything covered. We are bringing a new platform into our lab in 2 weeks, and I want to have everything ready to go. Leslie Graves Technical Specialist Cape Fear Valley Medical Center Fayetteville, NC 28341 910-615-6142 lgrav@capefearvalley.com On Mon, Jul 14, 2014 at 1:55 PM, Leslie Graves wrote: > Does anyone have a sample IHC Validation worksheet that they used in > their lab for review. I know that the requirements have changed since > our last inspection. I want to make sure that I am including all of > the criteria, and would like to see how other labs are documenting this process. > > Leslie Graves > Technical Specialist > Cape Fear Valley Medical Center > 1638 Owen Drive > Fayetteville, NC 28341 > 910-615-6142 > lgrav@capefearvalley.com > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Tue Jul 15 10:37:39 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Jul 15 10:38:04 2014 Subject: [Histonet] Re: IHC Validation In-Reply-To: References: Message-ID: I document all of the worksheet process? and then I create a ?Validation Report? where my technical director both reviews all slides and signs this report. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 7/15/14, 7:51 AM, "Leslie Graves" wrote: >Thank you to everyone who sent a template. This is very helpful. I do >need clarfication on the 10 cases that are required for validation. Do >you >include the 10 cases on this same form, or do you have something separate >for that? These look ideal for optimization. From what I have read >online, 10 cases need to be run after optimization has been completed. >When validating these 10 cases, accuracy, sensitivity, specificity and >reproducibility need to be monitored. Are all of you using separate form >to capture this data and approval from the Pathologists? Maybe I am going >overboard, but I want to make sure that I have everything covered. We are >bringing a new platform into our lab in 2 weeks, and I want to have >everything ready to go. > >Leslie Graves >Technical Specialist >Cape Fear Valley Medical Center >Fayetteville, NC 28341 >910-615-6142 >lgrav@capefearvalley.com > >On Mon, Jul 14, 2014 at 1:55 PM, Leslie Graves >wrote: > >> Does anyone have a sample IHC Validation worksheet that they used in >>their >> lab for review. I know that the requirements have changed since our >>last >> inspection. I want to make sure that I am including all of the >>criteria, >> and would like to see how other labs are documenting this process. >> >> Leslie Graves >> Technical Specialist >> Cape Fear Valley Medical Center >> 1638 Owen Drive >> Fayetteville, NC 28341 >> 910-615-6142 >> lgrav@capefearvalley.com >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cecystan76 <@t> gmail.com Tue Jul 15 12:26:17 2014 From: cecystan76 <@t> gmail.com (Cecilia A.) Date: Tue Jul 15 12:26:28 2014 Subject: [Histonet] doing histology in an academic research setting Message-ID: Hi everyone, I have been doing histology professionally for the past 5 years in an academic research lab setting (and an additional 5 years for my own grad studies). I take pride in the samples I have processed, cut, stained, and I love being able to troubleshoot any challenges or questions that the lab encounters. While I have this feeling for the actual work churned out, I can?t help but feel left out in lab meetings when actual data are presented and discussed. It makes me feel I don?t do enough. I guess what I?ve struggled with is that I do want to be a part of doing data procurement and analysis, but with the amount of work routine histology requires there?s not enough time in the day to also do microscope work and count for data. For those who are in a similar setting, do you share the same feeling? My apologies in advance if this is a silly thing to share/ask, but it has been bugging me for some time now. Thanks, Cecilia From mhanna <@t> histosearch.com Tue Jul 15 12:52:44 2014 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Tue Jul 15 12:52:51 2014 Subject: [Histonet] Image uploading In-Reply-To: <1405192652599.19189@kcl.ac.uk> References: <1405192652599.19189@kcl.ac.uk> Message-ID: <53C56A6C.2080806@histosearch.com> Hi Carl, I have sent you a username and password to be able to edit your image posts. Let me know if you have any problems or suggestions. You have definitely posted some great looking images. Best Regards, Marvin Hanna On 07/12/2014 03:17 PM, Hobbs, Carl wrote: > I make mistakes when I upload images.... > So, I'd be most grateful for an edit function to be included. > > Thanks for the opportunity to share images. > Sure, the excellent Immunoportal was The Best...I hope that Histonet can equal that website quality? > > Carlos > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Richard.Cartun <@t> hhchealth.org Tue Jul 15 13:48:40 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Tue Jul 15 13:48:51 2014 Subject: [Histonet] Antibodies to fungus Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355BC84F@HHCEXCHMB03.hhcsystem.org> I think I asked this question a few years ago, but I'm going to ask again ........ DAKO used to sell monoclonal antibodies to Aspergillus (clone Mab-WF-AF-1) and Rhizomucor (clone Mab-WSSA-RA-1). Unfortunately, they discontinued these reagents several years ago. Does anyone know if these antibodies are still available through another vendor? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From denise.long <@t> uconn.edu Tue Jul 15 14:02:10 2014 From: denise.long <@t> uconn.edu (Long, Denise) Date: Tue Jul 15 14:02:16 2014 Subject: [Histonet] Microtome calibration Message-ID: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 From denise.long <@t> uconn.edu Tue Jul 15 14:05:16 2014 From: denise.long <@t> uconn.edu (Long, Denise) Date: Tue Jul 15 14:05:22 2014 Subject: [Histonet] Control block validation requirements Message-ID: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F4122@MAILD.grove.ad.uconn.edu> Same inspectors advised our special stain and IHC controls should have at least two if not three different validation means (PCR, micro, serology, etc.) Anyone out there actually doing this? Just so you know, I've spoken with a few suppliers on control slides and they do not do this type of validation on their control material (surprise, surprise!) Thanks, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 From bburnett <@t> CapeCodHealth.org Tue Jul 15 14:26:55 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Tue Jul 15 14:27:00 2014 Subject: [Histonet] ER+/PR- in elderly patients Message-ID: I was wondering if anyone is seeing a trend in ER+/PR- tumors more frequent in older patients? If so, do you have any information regarding this? Thanks~ Brandy Burnett, HTL ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From rmire <@t> cvpath.org Tue Jul 15 14:55:18 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Tue Jul 15 14:55:44 2014 Subject: [Histonet] Microtome calibration In-Reply-To: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> Message-ID: Hi Denise, Check with the company that does your PM service. This may be something that is already done during preventative maintenance. On Jul 15, 2014, at 3:02 PM, "Long, Denise" wrote: > Hi Everyone, > During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. > Thank, > > Denise M. Long, MS, HTL (ASCP), QIHC > University of Connecticut > Dept. of Pathobiology and Veterinary Sciences > Connecticut Veterinary Medical Diagnostic Laboratory > 61 N. Eagleville Road, Unit 3089 > Storrs, Connecticut 06269-3089 > (860) 486-0851 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Tue Jul 15 15:14:20 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Jul 15 15:14:30 2014 Subject: [Histonet] Microtome calibration In-Reply-To: References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> Message-ID: We do ours by hand? You mean line them up or PM? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 7/15/14, 1:55 PM, "Ronda Mire" wrote: >Hi Denise, > >Check with the company that does your PM service. This may be something >that is already done during preventative maintenance. > >On Jul 15, 2014, at 3:02 PM, "Long, Denise" wrote: > >> Hi Everyone, >> During a recent inspection we were asked by the inspection team to >>start calibrating our microtomes. Anyone know of a service vendor that >>will do this? Referrals, including self-referrals would be greatly >>appreciated. >> Thank, >> >> Denise M. Long, MS, HTL (ASCP), QIHC >> University of Connecticut >> Dept. of Pathobiology and Veterinary Sciences >> Connecticut Veterinary Medical Diagnostic Laboratory >> 61 N. Eagleville Road, Unit 3089 >> Storrs, Connecticut 06269-3089 >> (860) 486-0851 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Tue Jul 15 15:29:47 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Jul 15 15:30:02 2014 Subject: [Histonet] Control block validation requirements In-Reply-To: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F4122@MAILD.grove.ad.uconn.edu> References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F4122@MAILD.grove.ad.uconn.edu> Message-ID: Denise, When selecting infectious agent positive controls, I also select cases which have been shown to be positive by other testing means (EM, Bacteriology, Virology, PCR). In addition, I run a cross-reactivity validation with my antibody on other infectious agent positive control slides to make sure my antibody is specific for the infectious agent and doesn't cross-react with other viruses/bacteria, etc. Results go into a table that is stored along with the initial antibody validation/optimization logsheet(s). When validating tissue antigens/tumor markers, I use the obvious normal anatomic cell/tissue control for that antigen. My anatomic pathologist advisor signs off on the final optimization for all antibodies before the IHC test can be put into public use. Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu On Tue, Jul 15, 2014 at 2:05 PM, Long, Denise wrote: > Same inspectors advised our special stain and IHC controls should have at > least two if not three different validation means (PCR, micro, serology, > etc.) > Anyone out there actually doing this? > Just so you know, I've spoken with a few suppliers on control slides and > they do not do this type of validation on their control material (surprise, > surprise!) > Thanks, > > Denise M. Long, MS, HTL (ASCP), QIHC > University of Connecticut > Dept. of Pathobiology and Veterinary Sciences > Connecticut Veterinary Medical Diagnostic Laboratory > 61 N. Eagleville Road, Unit 3089 > Storrs, Connecticut 06269-3089 > (860) 486-0851 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- From Joyce.Weems <@t> emoryhealthcare.org Tue Jul 15 15:34:59 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jul 15 15:35:08 2014 Subject: [Histonet] Microtome calibration In-Reply-To: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> Message-ID: Could they have meant an aligner - something like this? http://www.newcomersupply.com/products/microtome-cryostat-aligners?page=M#1130 Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Long, Denise Sent: Tuesday, July 15, 2014 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome calibration Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From smartin <@t> lcpath.com Tue Jul 15 15:46:44 2014 From: smartin <@t> lcpath.com (Suzanne Martin) Date: Tue Jul 15 15:46:48 2014 Subject: [Histonet] Temp full time job in SW WA Message-ID: <13bca1638c42034fa0840aead4ac6574@mail2.lcpath.com> We have a temporary full time position to fill. We are looking for a ASCP certified Histotech that can work from the beginning of September to the end of December/beginning of January. This would be full time coverage for a maternity leave. Hours 6-2:30. Qualifications: ASCP certified Histotech Some Histology work experience (preferred) Responsibilities: Embed Microtomy Manual Special Stains Immunohistochemistry Other duties as assigned Thank you! Suzanne Please respond to aamos@lcpath.com From histo <@t> pathlab.us Wed Jul 16 07:57:53 2014 From: histo <@t> pathlab.us (Histology) Date: Wed Jul 16 07:58:29 2014 Subject: [Histonet] slide dryer Message-ID: <566E7795BC94204D9A21539DE282AED70D5277@DC01.dp.local> Hi Histonetters, Will anyone share what type and brand of slide dryer they are using for routine H&Es? I would also like to know about how long it takes to melt the paraffin and how many racks you can do at a time. Thanks in advance, Mehndi Helgren Dominion Pathology Laboratories From mjones <@t> metropath.com Wed Jul 16 08:07:37 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Wed Jul 16 08:07:46 2014 Subject: [Histonet] Microtome calibration In-Reply-To: References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> Message-ID: We do ours by hand? You mean line them up or PM? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 7/15/14, 1:55 PM, "Ronda Mire" wrote: >Hi Denise, > >Check with the company that does your PM service. This may be something >that is already done during preventative maintenance. > >On Jul 15, 2014, at 3:02 PM, "Long, Denise" wrote: > >> Hi Everyone, >> During a recent inspection we were asked by the inspection team to >>start calibrating our microtomes. Anyone know of a service vendor that >>will do this? Referrals, including self-referrals would be greatly >>appreciated. >> Thank, >> >> Denise M. Long, MS, HTL (ASCP), QIHC >> University of Connecticut >> Dept. of Pathobiology and Veterinary Sciences >> Connecticut Veterinary Medical Diagnostic Laboratory >> 61 N. Eagleville Road, Unit 3089 >> Storrs, Connecticut 06269-3089 >> (860) 486-0851 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmoreira <@t> sidra.org Wed Jul 16 08:22:08 2014 From: jmoreira <@t> sidra.org (Joana Moreira) Date: Wed Jul 16 08:22:20 2014 Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 14 In-Reply-To: <09.6E.16637.08F55C35@smtp2.sidra.org> References: <09.6E.16637.08F55C35@smtp2.sidra.org> Message-ID: James, Hi! I used to use EDTA decalcification back in the UK and didn't deal with complaints from hematopathologists regarding that particular problem. The hospital where I worked didn't have the Milestone Bonestation but did use a temperature controlled oscillator performing the decalcification at 37C with oscillation for 48h. It's true it takes a lot longer to decalcify but the quality of IHC, ISH/FISH as well as DNA/RNA yield is far better. The decalcification process is published in a paper: Fluorescence in situ hybridisation analysis of bone marrow trephine biopsy specimens; an additional tool in the diagnostic armoury; that you can find at http://www.ncbi.nlm.nih.gov/pubmed/23038690. Your institution much probably has access to it but if you're interested and can't get the full version email me and I shall get that to you. Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Message: 3 Date: Mon, 14 Jul 2014 13:37:53 -0500 From: "Vickroy, Jim" Subject: [Histonet] Bone Marrow Trephine Cases To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Over the past several years we have had some questions by our hematopathologists regarding the quality of the trephine blocks. The biggest concern has been that the sections seem to have a fragmented appearance which we have determined in the past to be areas where the lipid cell borders break. We have found that the artifact is diminished when we pick the sections up as quickly as we can which led us to believe that the cell borders break when they are left on the warm water in the waterbath. Some of the pathologists still believe the artifact is caused by the decalcification process. I have reviewed protocols before and obviously found that labs use different decalcifying agents from a harsh HCL solution to a weaker formic acid solution. We currently use Immunocal which I believe is a formic acid solution. I have also been introduced to some new instrumentation available such as the Milestone Bone Station which uses a EDTA solution with some added heat and agitation. I realize that EDTA yields more DNA and RNA which is obviously an advantage however the length of time for the decal process with EDTA is much longer. I'm not sure that using EDTA for decalcification will however reduce the artifact that the pathologists see (fragmentation and breaking up of the lipid cells in the sections. I would appreciate any information that others might be able to share from their experiences and if they have dealt with a similar problem. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 217-415-7505 Cell ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From mucram11 <@t> comcast.net Wed Jul 16 09:13:12 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Jul 16 09:13:29 2014 Subject: [Histonet] Microtome calibration In-Reply-To: Message-ID: <1464440650.782.1405519992987.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We have a service contract with an annual PM.? The aligments and calibration are done then. Pam ----- Original Message ----- From: "Michael Ann Jones" To: "Ronda Mire" , "Denise Long" Cc: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 16, 2014 8:07:37 AM Subject: Re: [Histonet] Microtome calibration We do ours by hand? You mean line them up or PM? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 7/15/14, 1:55 PM, "Ronda Mire" wrote: >Hi Denise, > >Check with the company that does your PM service. ?This may be something >that is already done during preventative maintenance. > >On Jul 15, 2014, at 3:02 PM, "Long, Denise" wrote: > >> Hi Everyone, >> During a recent inspection we were asked by the inspection team to >>start calibrating our microtomes. ?Anyone know of a service vendor that >>will do this? Referrals, including self-referrals would be greatly >>appreciated. >> Thank, >> >> Denise M. Long, MS, HTL (ASCP), QIHC >> University of Connecticut >> Dept. of Pathobiology and Veterinary Sciences >> Connecticut Veterinary Medical Diagnostic Laboratory >> 61 N. Eagleville Road, Unit 3089 >> Storrs, Connecticut 06269-3089 >> (860) 486-0851 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BJohnson <@t> pbmlabs.com Wed Jul 16 11:17:32 2014 From: BJohnson <@t> pbmlabs.com (Brian Johnson) Date: Wed Jul 16 11:19:16 2014 Subject: [Histonet] Lewisville, TX Accessioning Supervisor Position Message-ID: Accessioning Supervisor We are currently seeking a supervisor for our Accessioning department. This is a working supervisor position and therefore the job incumbent will not only lead and direct a team of 5-7 employees, but will also be required to open/sort specimens, enter patient information into the Laboratory Information System (LIS), and perform other routine Accessioning Technician and problem resolution tasks. The ideal candidate will possess at least 3+ years of specimen accessioning experience, working with a full range of anatomic pathology tissue specimens, as well as 2+ years of previous supervisor experience. Knowledge of ATLAS and SunQuest CoPath a plus. Position is typically M-F, working mid-day shift, ie 11a-8p. Please contact wscoggins@pbmlabs.com PBM Labs PRIVACY AND CONFIDENTIALITY NOTICE: The information transmitted in this message and any attachments is intended for the person or entity to which it is addressed and may contain proprietary, confidential, and/or privileged material, which may include individually identifiable health information that requires safeguarding in compliance with the Health Insurance Portability and Accountability Act of 1996 as well as other federal and state laws. Any review, duplication, retransmission, distribution, or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you have received this message in error, please notify the sender immediately by return e-mail and delete all of the information from all computers or systems.? From tasega <@t> live.com Thu Jul 17 06:53:26 2014 From: tasega <@t> live.com (Marie) Date: Thu Jul 17 06:53:30 2014 Subject: [Histonet] looking for equipment Message-ID: Hi Histonet family, I'm looking for operational histology equipment or ones that might need minor repairs. If anyone wants to get rid of any, can you kindly inform me. I will take care of any shipping expenses. You may contact me Marie (HT)at 410-371-6051 or email me at tasega@live.com Thank you From joelleweaver <@t> hotmail.com Thu Jul 17 08:35:46 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Thu Jul 17 08:35:50 2014 Subject: [Histonet] looking for equipment In-Reply-To: References: Message-ID: Marie Get back in touch with me in about a month if you have not found what you need. I may have most of the basic items ( microtomes, H & E stainer, tissue processors, oven, hot plates, other stuff) up for grabs soon. Thank you Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tasega@live.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 17 Jul 2014 07:53:26 -0400 > Subject: [Histonet] looking for equipment > > Hi Histonet family, I'm looking for operational histology equipment or ones that might need minor repairs. If anyone wants to get rid of any, can you kindly inform me. I will take care of any shipping expenses. You may contact me Marie (HT)at 410-371-6051 or email me at tasega@live.com Thank you _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Jul 17 10:13:48 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jul 17 10:13:59 2014 Subject: [Histonet] RELIA Histology Careers Bulletin 7-15-2014 It's A Sizzlin Hot Summer!!! Exciting Opportunities for Certified and Uncertified Techs!! Message-ID: <02cb01cfa1d1$b6608790$232196b0$@earthlink.net> Hi Histonetters!! I hope you are having a great day. Are you keeping cool on these hot summer days? It's Summertime and it's Sizzlin' Hot and it's not just the temps!! I have some great new opportunities to tell you about: I have full time positions with the best employers in the country. Competitive salaries, great benefits & relocation assistance. Positions for Experienced Certified and Uncertified Techs!! My clients are ready to interview and hire today!! They are waiting to hear from me about candidates like you and your friends!! Here is a list of the Sizzlin Summer Stuff! Histotech - Modesto, CA Histotech - IL/IN border -near Chicago Lead Histology Tech - St. Louis, MO Histology Tech days - Madison, WI - temp to perm Histology Tech nights - Nashville, TN Histology/Necropsy Tech - Chicago, IL IHC Supervisor Night Supervisor - Dallas, TX Molecular Pathologists Analyst - Charlottesville, VA If you have any questions or know someone who might be interested please contact me. I would love to tell you about these opportunities or write another referral check like I did today if you have a friend who might be interested. I hope to hear from you soon. In the meantime grab some shade and a cool drink! I can be reached toll free at 866-607-3542 or on my cell at 407-353-5070 or via email at relia1@earthlink.net Have a great day!! Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mw <@t> personifysearch.com Thu Jul 17 13:23:08 2014 From: mw <@t> personifysearch.com (Matt Ward) Date: Thu Jul 17 13:23:14 2014 Subject: [Histonet] New Position Alert - Antibody Sales - NY/NJ/Eastern PA Message-ID: <0e6b01cfa1ec$299f1900$7cdd4b00$@personifysearch.com> Good Afternoon, We are currently searching for an Antibody Sales Specialist with a world leader in Cancer Diagnostics. Our client is looking for a histology professional to join the team to drive IHC Antibody Sales in the NY, NJ, and Eastern PA market. The ideal candidate will have IHC/Antibody experience - Sales experience is a plus. The position offers a competitive base salary, a very lucrative commission structure, company car, and full benefits. If this sounds like something you or someone you may know would be interested in, please contact me at mw@personifysearch.com . Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com From akbitting <@t> geisinger.edu Thu Jul 17 15:17:34 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Jul 17 15:17:42 2014 Subject: [Histonet] LOGOS One Message-ID: <77F52EFAB8B1694B885E277C48FCD0F679E68702@GHSEXMBX1W8K1V.geisinger.edu> If anyone is currently using this processor I'd love to hear some feedback on this specific model. Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From rmire <@t> cvpath.org Fri Jul 18 06:39:35 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Fri Jul 18 06:39:48 2014 Subject: [Histonet] looking for equipment In-Reply-To: References: Message-ID: <0FE00A77-FEC6-4C76-AC02-4A02FEC68C46@cvpath.org> Hi Marie, What are you looking for. I have some items. Ronda Mire CVPath Institute On Jul 17, 2014, at 9:35 AM, Joelle Weaver wrote: > Marie > Get back in touch with me in about a month if you have not found what you need. I may have most of the basic items ( microtomes, H & E stainer, tissue processors, oven, hot plates, other stuff) up for grabs soon. > > Thank you > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > >> From: tasega@live.com >> To: histonet@lists.utsouthwestern.edu >> Date: Thu, 17 Jul 2014 07:53:26 -0400 >> Subject: [Histonet] looking for equipment >> >> Hi Histonet family, I'm looking for operational histology equipment or ones that might need minor repairs. If anyone wants to get rid of any, can you kindly inform me. I will take care of any shipping expenses. You may contact me Marie (HT)at 410-371-6051 or email me at tasega@live.com Thank you _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From murphyv <@t> karmanos.org Fri Jul 18 09:44:13 2014 From: murphyv <@t> karmanos.org (Murphy, Valerie) Date: Fri Jul 18 09:44:24 2014 Subject: [Histonet] Brain tissue coming off slide Message-ID: We are staining human FFPE brain sections for Her 2 Neu and we're having a problem with tissue coming off the slide during the HIER step. We are currently using Vectabond coated plus slides and using a decloaking chamber for HIER. Are there any other steps we can take to prevent tissue coming off the slide? Thank you, Valerie Ratliff Karmanos Cancer Institute ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. From liz <@t> premierlab.com Fri Jul 18 10:16:04 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Jul 18 10:16:16 2014 Subject: [Histonet] RE: Brain tissue coming off slide In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD373@SBS2K8.premierlab.local> Valerie There are a few things that you can try. 1. This may or may not work with the Her2 IHC, since for that antibody I believe it requires retrieval at least at 98C but time and temperature are related with respects to heat retrieval. You could try 70C for 2 hours this is gentler on the tissue and works for some antibodies but not all of them. For example it does not work for Ki-67 in our hands. We use this retrieval method on bone and other types of friable tissue if necessary. 2. We have found that some samples after HIER may require manual staining methods in order to optimize tissue adherence, not sure if that is an option for you too. 3. Post fixation in 10% NBF after deparaffinization for about 10% minutes. This may help tissue adherence but we have also found that this may decrease the sensitivity of the protocol and you may have to adjust your primary antibody concentration. 4. Tissue adherence to the slide especially with brain (we have seen this on rodent brains) requires proper fixation and adequate processing, poorly fixed or processed brain samples will have issues with tissue adherence. 5. Allow the cut slides to sit for about a week prior to staining we find this may help with tissue adherence. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Murphy, Valerie Sent: Friday, July 18, 2014 8:44 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Brain tissue coming off slide We are staining human FFPE brain sections for Her 2 Neu and we're having a problem with tissue coming off the slide during the HIER step. We are currently using Vectabond coated plus slides and using a decloaking chamber for HIER. Are there any other steps we can take to prevent tissue coming off the slide? Thank you, Valerie Ratliff Karmanos Cancer Institute ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From murphyv <@t> karmanos.org Fri Jul 18 11:00:26 2014 From: murphyv <@t> karmanos.org (Murphy, Valerie) Date: Fri Jul 18 11:00:34 2014 Subject: [Histonet] RE: Brain tissue coming off slides Message-ID: Thank you all for the great tips! I will try your suggestions. Valerie Ratliff Karmanos Cancer Institute ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. From Karen.Heckford <@t> DignityHealth.org Fri Jul 18 13:04:13 2014 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Fri Jul 18 13:04:21 2014 Subject: [Histonet] Pathnet program Message-ID: Hi, Any of you using the Pathnet program. We just went live and now they are telling me that our labels will not work with the program. We are currently use 7/8"x7/8" square labels 4 across Stainershield labels. I don't understand why they will not work? We did use the Powerpath 9.2 program before. Can't we just go into "properties" and change it to work? Any input would be greatly appreciated. Thanks, Karen Heckford HT ASCP From patpxs <@t> gmail.com Fri Jul 18 15:11:22 2014 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Fri Jul 18 15:11:26 2014 Subject: [Histonet] Clinical EM Labs? Message-ID: Hello Netters and Listers, I was wondering how many clinical EM labs there are in the US? I get the feeling that we are becoming a small group and just wanted to see who else is out there. My lab has two services: surgical pathology and diagnostic virology. Please contact me if you are clinical EM as well. I figured, if we are a small group, we should get to know each other. Thanks and have a nice weekend. Sincerely, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From j.rowaihi <@t> alborglaboratories.com Sat Jul 19 05:42:08 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Sat Jul 19 05:39:49 2014 Subject: [Histonet] Validation of IHC Message-ID: <013901cfa33e$17ff7070$47fe5150$@rowaihi@alborglaboratories.com> Dear colleagues We purchased Ventana GX from Rosh for IHC I reviewed the resent CAP check list 2013 for validation of IHC but I got confused. I am using ready to use prediluted antibodies from Ventana and I want to validate the antibodies before use according to CAP requirements. Can I have the validation plane and procedure for IHC antibodies. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From rjbuesa <@t> yahoo.com Sat Jul 19 09:31:30 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 19 09:31:35 2014 Subject: [Histonet] Brain tissue coming off slide In-Reply-To: References: Message-ID: <1405780290.86815.YahooMailNeo@web120404.mail.ne1.yahoo.com> Generally speaking, brain tissue is likely to fell off if: 1- the section is too thick or "wavy", or if 2- the tissue has some infiltration problems and brain is one tissue difficult to process/infiltrate correctly. Besides any problems with temp. during HIER or the type of adhesive or type of slide you use, please take a look at your protocol especially if the sections fall off during HIER and not during routine staining. Ren? J.? On Friday, July 18, 2014 10:44 AM, "Murphy, Valerie" wrote: We are staining human FFPE brain sections for Her 2 Neu and we're having a problem with tissue coming off the slide during the HIER step. We are currently using Vectabond coated plus slides and using a decloaking chamber for HIER. Are there any other steps we can take to prevent? tissue coming off the slide? Thank you, Valerie Ratliff Karmanos Cancer Institute ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Jul 19 09:36:34 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 19 09:36:49 2014 Subject: [Histonet] Clinical EM Labs? In-Reply-To: References: Message-ID: <1405780594.28405.YahooMailNeo@web120406.mail.ne1.yahoo.com> I have some experiences in asking such type of general questions to Histonet members and frequently the answers are few and far apart. I think that you should prepare a very short questionnaire and give it out at the next NSH meeting. Perhaps the?NSH could have some idea or the National Health Institute or the National Academy of Sciences. You are right when writing that EM labs are a "dying breed". We used to be and no longer are. Ren? J.? On Friday, July 18, 2014 4:11 PM, Paula Sicurello wrote: Hello Netters and Listers, I was wondering how many clinical EM labs there are in the US?? I get the feeling that we are becoming a small group and just wanted to see who else is out there. My lab has two services:? surgical pathology and diagnostic virology. Please contact me if you are clinical EM as well. I figured, if we are a small group, we should get to know each other. Thanks and have a nice weekend. Sincerely, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P:? 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rebecca.Riesen <@t> hma.com Sat Jul 19 10:41:04 2014 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Sat Jul 19 10:41:14 2014 Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 17 In-Reply-To: <19740415104537.382285048BA7ABDB@mx1.hma.com> References: <19740415104537.382285048BA7ABDB@mx1.hma.com> Message-ID: Liz had some great suggestions! The one that I have found to be the MOST important is #4. Standing my slides up at a slight angle and allowing the moisture to drain out from under the section AND then letting the drained slides sit and dry for a good long time is extremely important for adherance to be successful (I've never gone as far as a week, although it couldn't hurt, but at least overnight seems necessary). I would focus on that, so you first get good adherance and then the other tips following the proper drying will then help keep them on. Good luck! ------------------------------ Message: 4 Date: Fri, 18 Jul 2014 14:44:13 +0000 From: "Murphy, Valerie" Subject: [Histonet] Brain tissue coming off slide To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" We are staining human FFPE brain sections for Her 2 Neu and we're having a problem with tissue coming off the slide during the HIER step. We are currently using Vectabond coated plus slides and using a decloaking chamber for HIER. Are there any other steps we can take to prevent tissue coming off the slide? Thank you, Valerie Ratliff Karmanos Cancer Institute ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. ------------------------------ Message: 5 Date: Fri, 18 Jul 2014 09:16:04 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: Brain tissue coming off slide To: "Murphy, Valerie" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD373@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Valerie There are a few things that you can try. 1. This may or may not work with the Her2 IHC, since for that antibody I believe it requires retrieval at least at 98C but time and temperature are related with respects to heat retrieval. You could try 70C for 2 hours this is gentler on the tissue and works for some antibodies but not all of them. For example it does not work for Ki-67 in our hands. We use this retrieval method on bone and other types of friable tissue if necessary. 2. We have found that some samples after HIER may require manual staining methods in order to optimize tissue adherence, not sure if that is an option for you too. 3. Post fixation in 10% NBF after deparaffinization for about 10% minutes. This may help tissue adherence but we have also found that this may decrease the sensitivity of the protocol and you may have to adjust your primary antibody concentration. 4. Tissue adherence to the slide especially with brain (we have seen this on rodent brains) requires proper fixation and adequate processing, poorly fixed or processed brain samples will have issues with tissue adherence. 5. Allow the cut slides to sit for about a week prior to staining we find this may help with tissue adherence. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com From gayle.callis <@t> bresnan.net Sat Jul 19 11:06:06 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Sat Jul 19 11:06:50 2014 Subject: [Histonet] Request for copy of old Technicon operation manual Message-ID: <000001cfa36b$5a8a6080$0f9f2180$@bresnan.net> Dear All, A friend is donating her old Technicon to be used in another country, and needs a copy of the operation manual. If anyone still has this, could they send a scanned pdf copy to me asap. I will forward it to person in need Thank you in advance Gayle Callis HTL/HT/MT(ASCP) From j.rowaihi <@t> alborglaboratories.com Sun Jul 20 03:40:44 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Sun Jul 20 03:38:19 2014 Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 17 In-Reply-To: References: <19740415104537.382285048BA7ABDB@mx1.hma.com> Message-ID: <019a01cfa3f6$4d6264d0$e8272e70$@rowaihi@alborglaboratories.com> I totally agree with you in step 4, and I think the tissue was not fixed probably or not processed in good solutions, my suggestion for the time now problem is to stain the slides manual. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Riesen, Rebecca Sent: Saturday, July 19, 2014 6:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 17 Liz had some great suggestions! The one that I have found to be the MOST important is #4. Standing my slides up at a slight angle and allowing the moisture to drain out from under the section AND then letting the drained slides sit and dry for a good long time is extremely important for adherance to be successful (I've never gone as far as a week, although it couldn't hurt, but at least overnight seems necessary). I would focus on that, so you first get good adherance and then the other tips following the proper drying will then help keep them on. Good luck! ------------------------------ Message: 4 Date: Fri, 18 Jul 2014 14:44:13 +0000 From: "Murphy, Valerie" Subject: [Histonet] Brain tissue coming off slide To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" We are staining human FFPE brain sections for Her 2 Neu and we're having a problem with tissue coming off the slide during the HIER step. We are currently using Vectabond coated plus slides and using a decloaking chamber for HIER. Are there any other steps we can take to prevent tissue coming off the slide? Thank you, Valerie Ratliff Karmanos Cancer Institute ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. ------------------------------ Message: 5 Date: Fri, 18 Jul 2014 09:16:04 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: Brain tissue coming off slide To: "Murphy, Valerie" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD373@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Valerie There are a few things that you can try. 1. This may or may not work with the Her2 IHC, since for that antibody I believe it requires retrieval at least at 98C but time and temperature are related with respects to heat retrieval. You could try 70C for 2 hours this is gentler on the tissue and works for some antibodies but not all of them. For example it does not work for Ki-67 in our hands. We use this retrieval method on bone and other types of friable tissue if necessary. 2. We have found that some samples after HIER may require manual staining methods in order to optimize tissue adherence, not sure if that is an option for you too. 3. Post fixation in 10% NBF after deparaffinization for about 10% minutes. This may help tissue adherence but we have also found that this may decrease the sensitivity of the protocol and you may have to adjust your primary antibody concentration. 4. Tissue adherence to the slide especially with brain (we have seen this on rodent brains) requires proper fixation and adequate processing, poorly fixed or processed brain samples will have issues with tissue adherence. 5. Allow the cut slides to sit for about a week prior to staining we find this may help with tissue adherence. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> skm.org.pk Mon Jul 21 05:47:37 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Mon Jul 21 05:48:15 2014 Subject: [Histonet] Validation of IHC In-Reply-To: <013901cfa33e$17ff7070$47fe5150$@rowaihi@alborglaboratories.com> References: <013901cfa33e$17ff7070$47fe5150$@rowaihi@alborglaboratories.com> Message-ID: Dear Jamal, Please see Principles of Analytic Validation of Immunohistochemistry Assays Published : Archives of Pathology and Laboratory Medicine March 19, 2014 cap.org Later on I will send you template of IHC Validation, which we are using in SKMCH&RC. Regards Muhammad Tahseen Senior Supervisor Histopathology SKMCH&RC Lahore, Pakistan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jamal Sent: Saturday, July 19, 2014 3:42 PM To: Histonet edu Subject: [Histonet] Validation of IHC Dear colleagues We purchased Ventana GX from Rosh for IHC I reviewed the resent CAP check list 2013 for validation of IHC but I got confused. I am using ready to use prediluted antibodies from Ventana and I want to validate the antibodies before use according to CAP requirements. Can I have the validation plane and procedure for IHC antibodies. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LTurner1 <@t> seton.org Mon Jul 21 06:11:17 2014 From: LTurner1 <@t> seton.org (Turner, Leandra) Date: Mon Jul 21 06:11:42 2014 Subject: [Histonet] Clinical EM Labs? In-Reply-To: <1405780594.28405.YahooMailNeo@web120406.mail.ne1.yahoo.com> References: <1405780594.28405.YahooMailNeo@web120406.mail.ne1.yahoo.com> Message-ID: I definitely have to agree about the "dying breed". Our hospital network closed down our whole specialty lab which included EM. It was very sad day when I had to turn off the scope for the last time. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, July 19, 2014 9:37 AM To: Paula Sicurello; HistoNet; Microscopy@microscopy.com Subject: Re: [Histonet] Clinical EM Labs? I have some experiences in asking such type of general questions to Histonet members and frequently the answers are few and far apart. I think that you should prepare a very short questionnaire and give it out at the next NSH meeting. Perhaps the?NSH could have some idea or the National Health Institute or the National Academy of Sciences. You are right when writing that EM labs are a "dying breed". We used to be and no longer are. Ren? J.? On Friday, July 18, 2014 4:11 PM, Paula Sicurello wrote: Hello Netters and Listers, I was wondering how many clinical EM labs there are in the US?? I get the feeling that we are becoming a small group and just wanted to see who else is out there. My lab has two services:? surgical pathology and diagnostic virology. Please contact me if you are clinical EM as well. I figured, if we are a small group, we should get to know each other. Thanks and have a nice weekend. Sincerely, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P:? 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From KSimeone <@t> leavittmgt.com Mon Jul 21 07:13:35 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon Jul 21 07:13:46 2014 Subject: [Histonet] FT DERM POSITION DELRAY BEACH, FLORIDA In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C2FDA00FF@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C2FD9FC68@vm-email.leavittmgt.com>, <43944B1DBAAC2846B7B9D626B5F1233C2FDA00FF@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C2FDA02BE@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Derm Lab. This is a permanent full time position with benefits (medical/401k/vacation). DRUG FREE WORKPLACE. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann@leavittmgt.com if interested. *full time position Mon-Fri 11a-7:30p (hours NON NEGOTIABLE) *MUST be licensed as a FL histotechnologist or technician *MUST have at least 2 years experience (dermatology preferred) *VERY proficient in embedding and microtomy *experience in grossing and immunohistochemistry (BOND) a plus *must be self motivated and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Richard.Cartun <@t> hhchealth.org Mon Jul 21 07:31:36 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Jul 21 07:31:42 2014 Subject: [Histonet] Clinical EM Labs? In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355BF64B@HHCEXCHMB03.hhcsystem.org> We closed our EM laboratory many years ago after our Director passed away unexpectedly and we could not find anyone to take his place. We now send all of our medical and transplant kidney biopsies to Columbia in NYC for diagnosis including EM. Other specimens (e.g., ciliary biopsies, heart biopsies for amyloid, an occasional liver biopsy, etc.) are sent to Yale. Although we run a large Anatomic Pathology operation there are very few specimens that require EM. We no longer use EM for tumors and most infectious disease cases are resolved with IHC. It's unfortunate (given our size) that we can no longer provide these services on-site; however, I'm a firm believer of "Centers of Excellence" and I think sending these specimens out to centers that are performing EM on a daily basis is best for the patients that we serve. Richard Cartun ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Paula Sicurello [patpxs@gmail.com] Sent: Friday, July 18, 2014 4:11 PM To: HistoNet; Microscopy@microscopy.com Subject: [Histonet] Clinical EM Labs? Hello Netters and Listers, I was wondering how many clinical EM labs there are in the US? I get the feeling that we are becoming a small group and just wanted to see who else is out there. My lab has two services: surgical pathology and diagnostic virology. Please contact me if you are clinical EM as well. I figured, if we are a small group, we should get to know each other. Thanks and have a nice weekend. Sincerely, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From pruegg <@t> ihctech.net Mon Jul 21 13:06:42 2014 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Jul 21 13:06:45 2014 Subject: [Histonet] HS IHC project Message-ID: Friends, I am going to help a local HS class (a Biotech class) do a simple IHC project. This class already processes tissue, embeds, sections and does an H&E stain. My husband fixed up an old black AO microtome for them to use and they actually cut sections. They cannot use human tissue there so what they have available is mouse spleen already ffpe. I was thinking of using a rab antibody that works on ffpe ms spleen. Ki67 comes to mind? It has been a while since I was in the lab so I am consulting with you all to make sure a rab poly or rab mono Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this can you tell me which ab was used successfully. If you can think of a better rab ab to use on the ffpe ms spleen for this project let me know. It would help if we could keep it simple for antigen retrieval. One of our kind vendors will be donating IHC reagents for this HS class project. I hope the IHC meeting is going well in Vegas, wish I was there. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From JMacDonald <@t> mtsac.edu Mon Jul 21 13:19:21 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jul 21 13:19:28 2014 Subject: [Histonet] HS IHC project In-Reply-To: References: Message-ID: T cell and B cell markers work well with human spleen. From: Patsy Ruegg To: "Histonet@Lists. Edu" Date: 07/21/2014 11:08 AM Subject: [Histonet] HS IHC project Sent by: histonet-bounces@lists.utsouthwestern.edu Friends, I am going to help a local HS class (a Biotech class) do a simple IHC project. This class already processes tissue, embeds, sections and does an H&E stain. My husband fixed up an old black AO microtome for them to use and they actually cut sections. They cannot use human tissue there so what they have available is mouse spleen already ffpe. I was thinking of using a rab antibody that works on ffpe ms spleen. Ki67 comes to mind? It has been a while since I was in the lab so I am consulting with you all to make sure a rab poly or rab mono Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this can you tell me which ab was used successfully. If you can think of a better rab ab to use on the ffpe ms spleen for this project let me know. It would help if we could keep it simple for antigen retrieval. One of our kind vendors will be donating IHC reagents for this HS class project. I hope the IHC meeting is going well in Vegas, wish I was there. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Mon Jul 21 17:55:35 2014 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Mon Jul 21 17:55:38 2014 Subject: [Histonet] HS IHC project In-Reply-To: References: Message-ID: I am so jealous of these kids. I didn't get near a microscope until college, let alone a microtome. I hope they have fun! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Jul 21, 2014 at 2:19 PM, Jennifer MacDonald wrote: > T cell and B cell markers work well with human spleen. > > > > From: Patsy Ruegg > To: "Histonet@Lists. Edu" > Date: 07/21/2014 11:08 AM > Subject: [Histonet] HS IHC project > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > Friends, > > I am going to help a local HS class (a Biotech class) do > a simple IHC project. This class already processes tissue, embeds, > sections and does an H&E stain. My husband fixed up an old black AO > microtome for them to use and they actually cut sections. They cannot > use human tissue there so what they have available is mouse spleen > already ffpe. I was thinking of using a rab antibody that works on ffpe > ms spleen. Ki67 comes to mind? It has been a while since I was in the > lab so I am consulting with you all to make sure a rab poly or rab mono > Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this > can you tell me which ab was used successfully. If you can think of a > better rab ab to use on the ffpe ms spleen for this project let me > know. It would help if we could keep it simple for antigen retrieval. > > One of our kind vendors will be donating IHC reagents for this HS class > project. > > I hope the IHC meeting is going well in Vegas, wish I was there. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > pruegg@ihctech.net > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From durynski <@t> vet.upenn.edu Tue Jul 22 11:14:12 2014 From: durynski <@t> vet.upenn.edu (Karie Durynski) Date: Tue Jul 22 11:14:30 2014 Subject: [Histonet] Procedure on waxing a tibia for presentation Message-ID: <808213354.68140.1406045652054.JavaMail.root@zimbra.upenn.edu> Hello All, I am looking for a procedure to wax/coat with acrylic for a tibia to use for display, presentation and teaching. Thank you in advance, Karie -- Karie L Durynski New Bolton Center University of Pennsylvania School of Veterinary Medicine Comparative Orthopaedic Research Laboratory Histology Section Supervisor 382 W Street Road Kennett Square, PA. 19348 Phone:610-925-6278 Fax:610-925-6820 Email:durynski@vet.upenn.edu From joewalker <@t> rrmc.org Tue Jul 22 11:29:44 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Tue Jul 22 11:29:50 2014 Subject: [Histonet] RE: Pathnet program In-Reply-To: References: Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC1857ED5F@RRMBX01.rrmc.local> HI Karen, We use pathnet for our label printing. Cerner issues a "standard" label program that will only print the information on the label when the labels do not contain a small horizontal gap between the four labels going across the roll. We too had to switch to a different label in order to meet Cerner's standard. The funny thing is that the label standard is to have a small gap between the labels. To have no gap is a customization or at least that is the case with our label vendors. Due to the cost of the custom label rolls, we have request a "custom" label program from Cerner so that it will accept the standard label with a small gap. The custom request is a 1 time expense vs. the monthly expense on the label. You can find the CCL customization information on Cerner's UCERN website. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Friday, July 18, 2014 2:04 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Pathnet program Hi, Any of you using the Pathnet program. We just went live and now they are telling me that our labels will not work with the program. We are currently use 7/8"x7/8" square labels 4 across Stainershield labels. I don't understand why they will not work? We did use the Powerpath 9.2 program before. Can't we just go into "properties" and change it to work? Any input would be greatly appreciated. Thanks, Karen Heckford HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From shive003 <@t> umn.edu Tue Jul 22 11:58:49 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Jul 22 11:58:57 2014 Subject: [Histonet] Clinical EM Labs? In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355BF64B@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E355BF64B@HHCEXCHMB03.hhcsystem.org> Message-ID: There is a listserver which is similar to Histonet for EM providers/investigators: http://www.microscopy.com/ This may provide some networking possibilities for those who are looking for EM lab information. Jan Shivers Veterinary Diagnostic Laboratory University of Minnesota On Mon, Jul 21, 2014 at 7:31 AM, Cartun, Richard < Richard.Cartun@hhchealth.org> wrote: > We closed our EM laboratory many years ago after our Director passed away > unexpectedly and we could not find anyone to take his place. We now send > all of our medical and transplant kidney biopsies to Columbia in NYC for > diagnosis including EM. Other specimens (e.g., ciliary biopsies, heart > biopsies for amyloid, an occasional liver biopsy, etc.) are sent to Yale. > Although we run a large Anatomic Pathology operation there are very few > specimens that require EM. We no longer use EM for tumors and most > infectious disease cases are resolved with IHC. It's unfortunate (given > our size) that we can no longer provide these services on-site; however, > I'm a firm believer of "Centers of Excellence" and I think sending these > specimens out to centers that are performing EM on a daily basis is best > for the patients that we serve. > > Richard Cartun > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of Paula Sicurello [ > patpxs@gmail.com] > Sent: Friday, July 18, 2014 4:11 PM > To: HistoNet; Microscopy@microscopy.com > Subject: [Histonet] Clinical EM Labs? > > Hello Netters and Listers, > > I was wondering how many clinical EM labs there are in the US? I get the > feeling that we are becoming a small group and just wanted to see who else > is out there. > > My lab has two services: surgical pathology and diagnostic virology. > > Please contact me if you are clinical EM as well. > > I figured, if we are a small group, we should get to know each other. > > Thanks and have a nice weekend. > > Sincerely, > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mtighe <@t> trudeauinstitute.org Tue Jul 22 12:17:04 2014 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Tue Jul 22 12:17:14 2014 Subject: [Histonet] Tissue clearing with scaleA2 reagent Message-ID: <1406049655015.27493@trudeauinstitute.org> Anyone using ScaleA2 reagent to clear tissues for imaging? I am wondering why the method requires Sucrose gradient followed by OCT embedding and freezing just to turn around and thaw and clear? From spapa <@t> wellspan.org Wed Jul 23 11:06:42 2014 From: spapa <@t> wellspan.org (Papa, Sally) Date: Wed Jul 23 11:07:17 2014 Subject: [Histonet] Fungus Control Tissue Message-ID: <1406131602337.16091@wellspan.org> I am looking for tissue to use for our GMS and PAS/Fungus stains, this tissue should be possitve for hyphae-form organisms. Thank you, Sally Papa BS,HTL(ASCP) Histology York Hospital York, PA 17404 ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ From cecystan76 <@t> gmail.com Wed Jul 23 16:46:02 2014 From: cecystan76 <@t> gmail.com (Cecilia A.) Date: Wed Jul 23 16:46:08 2014 Subject: [Histonet] Laser Microdissection on FFPE Message-ID: Hello everyone, I was wondering if anyone has ever tried to do laser microdissection on mouse FFPE tissue sections. The cell number yield we expect to have will be around 100 cells for RNA extraction (challenge #1). The next challenge is that our specimen would have been in fixation for more than 24 hours (10% NBF) whereby the starting material should have been fixed no more than 24 hours. Thank you for your time. Sincerely, Cecilia From jshelley <@t> sanfordburnham.org Thu Jul 24 08:11:25 2014 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Thu Jul 24 08:11:30 2014 Subject: [Histonet] Mouse on Mouse Staining Message-ID: Good Morning, I have avoided for the last few years doing IHC with mouse on mouse antibodies, but have been requested to do so at this time. I have two antibodies, HDAC1 and Nitrotyrosine from Santa Cruz that I am working up, however the staining pattern in my tissue does not seem to be revealing the accurate staining pattern. I was hoping someone in the research community has worked with these antibodies, even if from other vendors and would be willing to discuss further with me. Thanks!!! Kind Regards! John J Shelley Research Specialist, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona From jbro22 <@t> lsuhsc.edu Thu Jul 24 10:11:52 2014 From: jbro22 <@t> lsuhsc.edu (Browning, Jeffrey A.) Date: Thu Jul 24 10:11:56 2014 Subject: [Histonet] FT Day-Shift Histotech Openings - Certification not Required! Message-ID: <2E3D5719BC42374E982EF0AD611F2362B857E44F@SH-ExchMB1.master.lsuhsc.edu> Histonetters, University Health in Shreveport, LA is currently offering two full-time histotechnologist positions. Openings are for day shift Monday-Friday with opportunity for weekend/holiday call pay. Pay and benefits are regionally competitive. Applicants have the opportunity to acquire specialized skills in immunohistochemistry, cryotomy, immunofluorescence, and enzyme histochemistry. Certification is not required! Applications may be submitted here. Thanks! Jeff Browning, HTL(ASCP) Technical Director, Anatomic Pathology Department of Pathology LSU Health Science Center - Shreveport 1501 Kings Highway Shreveport, LA 71103 (318) 675-5872 jbro22@lsuhsc.edu From dawud_hilliard <@t> med.unc.edu Thu Jul 24 11:49:30 2014 From: dawud_hilliard <@t> med.unc.edu (Hilliard, Dawud Deshawn) Date: Thu Jul 24 12:00:03 2014 Subject: [Histonet] Xylene Substitute Protocols Message-ID: Hello all, Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success. I greatly appreciate the insight! Dawud Hilliard MPM, HTL (ASCP) QIHC Facility Director Animal Histopathology Core Lab Lineberger Comprehensive Cancer Center University of North Carolina - Chapel Hill Chapel Hill, North Carolina 27599 (919) 966-3653 From Terra.Wineman <@t> novusint.com Thu Jul 24 12:06:53 2014 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Thu Jul 24 12:07:02 2014 Subject: [Histonet] RE: Xylene Substitute Protocols In-Reply-To: References: Message-ID: <1EB8F245A303564EADF12AC7022FA74DCA953F08@novus-ex01> We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type Terra Wineman, HTL(ASCP)cm Novus International -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshawn Sent: Thursday, July 24, 2014 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute Protocols Hello all, Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success. I greatly appreciate the insight! Dawud Hilliard MPM, HTL (ASCP) QIHC Facility Director Animal Histopathology Core Lab Lineberger Comprehensive Cancer Center University of North Carolina - Chapel Hill Chapel Hill, North Carolina 27599 (919) 966-3653 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shklei <@t> bellin.org Thu Jul 24 12:59:13 2014 From: shklei <@t> bellin.org (Shannon Kleiner) Date: Thu Jul 24 12:59:28 2014 Subject: [Histonet] RE: Xylene Substitute Protocols In-Reply-To: <1EB8F245A303564EADF12AC7022FA74DCA953F08@novus-ex01> References: <1EB8F245A303564EADF12AC7022FA74DCA953F08@novus-ex01> Message-ID: <53D103210200008100008DD9@mail.bellin.org> Formula 83 in first three stations and last four stations on the Leica XL stainer. >>> "Wineman, Terra" 7/24/2014 12:06 PM >>> We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type Terra Wineman, HTL(ASCP)cm Novus International -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshawn Sent: Thursday, July 24, 2014 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute Protocols Hello all, Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success. I greatly appreciate the insight! Dawud Hilliard MPM, HTL (ASCP) QIHC Facility Director Animal Histopathology Core Lab Lineberger Comprehensive Cancer Center University of North Carolina - Chapel Hill Chapel Hill, North Carolina 27599 (919) 966-3653 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shklei <@t> bellin.org Thu Jul 24 13:00:34 2014 From: shklei <@t> bellin.org (Shannon Kleiner) Date: Thu Jul 24 13:00:45 2014 Subject: [Histonet] RE: Xylene Substitute Protocols References: <1EB8F245A303564EADF12AC7022FA74DCA953F08@novus-ex01> Message-ID: <53D103720200008100008DE3@mail.bellin.org> Richard Allen mounting media REF 4111. >>> Shannon Kleiner 7/24/2014 12:59 PM >>> Formula 83 in first three stations and last four stations on the Leica XL stainer. >>> "Wineman, Terra" 7/24/2014 12:06 PM >>> We use Richard Allan Clear-Rite 5min * 3 stations with any xylene based mounting type Terra Wineman, HTL(ASCP)cm Novus International -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshawn Sent: Thursday, July 24, 2014 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute Protocols Hello all, Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success. I greatly appreciate the insight! Dawud Hilliard MPM, HTL (ASCP) QIHC Facility Director Animal Histopathology Core Lab Lineberger Comprehensive Cancer Center University of North Carolina - Chapel Hill Chapel Hill, North Carolina 27599 (919) 966-3653 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Pawlowski <@t> jefferson.edu Thu Jul 24 13:06:26 2014 From: Mark.Pawlowski <@t> jefferson.edu (Mark Pawlowski) Date: Thu Jul 24 13:06:41 2014 Subject: [Histonet] Midwest Lab Equipment Message-ID: <1406225185926.90726@jefferson.edu> Hello, Has anyone had an experience with this company buying or selling used lab equipment? Mark The information contained in this transmission contains privileged and confidential information. It is intended only for the use of the person named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. CAUTION: Intended recipients should NOT use email communication for emergent or urgent health care matters. From gu.lang <@t> gmx.at Thu Jul 24 13:07:18 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jul 24 13:07:30 2014 Subject: [Histonet] problems Sllde Mate print Message-ID: <001501cfa76a$1b680e90$52382bb0$@gmx.at> Dear histonetters, we have problems with the stability of the print of our slide mates (Thermo). We use regular slides with frosted end. The print usually looks good and stable at the time of printing. But after staining the print comes off like peeled skin. Very easy to remove. We take care, that the print doesn't come in contact with organic solvents (deparaffination reagens = Histo SAV, butyl acetate), but without any logical reason the first line of the print peels off and the second and third line is rather ok. Has anyone seen similar problems and solved them? Gudrun Lang From wbenton <@t> cua.md Thu Jul 24 13:26:53 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Jul 24 13:27:03 2014 Subject: [Histonet] problems Sllde Mate print In-Reply-To: <001501cfa76a$1b680e90$52382bb0$@gmx.at> References: <001501cfa76a$1b680e90$52382bb0$@gmx.at> Message-ID: <0B8979A204680A42B93A52B486088CD93938636A02@CUAEXH1.GCU-MD.local> Gudrun, We have 2 slide mates and we run our slides through all reagents and do not have the issue you describe. The only issue we experienced was poor print quality due to the type of paint on the frosted end of the slides. I recently switched to Leica's charged slide that is designed for Thermal Printers like the slide mate and the print quality is top notch and does not fade, peel, or smear when touched and ran through xylene, alcohol, retrieval solutions etc.... Part # 3800090 SLIDE WHITE Xtra Thermal Are your ribbons old? Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.lang@gmx.at] Sent: Thursday, July 24, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problems Sllde Mate print Dear histonetters, we have problems with the stability of the print of our slide mates (Thermo). We use regular slides with frosted end. The print usually looks good and stable at the time of printing. But after staining the print comes off like peeled skin. Very easy to remove. We take care, that the print doesn't come in contact with organic solvents (deparaffination reagens = Histo SAV, butyl acetate), but without any logical reason the first line of the print peels off and the second and third line is rather ok. Has anyone seen similar problems and solved them? Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From wsimons <@t> athensgastro.com Thu Jul 24 13:33:05 2014 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Thu Jul 24 13:33:10 2014 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gTWlkd2VzdCBMYWIgRXF1aXBtZW50?= Message-ID: <20140724183305.1132.qmail@server276.com> Haven't heard of them and no experience with them.?? But I have dealt with IMEB out of San Diego when setting up labs. Excellent customer service in both buying and selling.??I usually call on Margaret or Charlie. Wanda >??-------Original Message------- >??From: Mark Pawlowski >??To: histonet@lists.utsouthwestern.edu >??Subject: [Histonet] Midwest Lab Equipment >??Sent: Jul 24 '14 14:06 >?? >??Hello, >?? >?? >?? >??Has anyone had an experience with this company buying or selling used lab equipment? >?? >?? >?? >??Mark >?? >??The information contained in this transmission contains privileged and confidential information. It is intended only for the use of the person named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. >?? >??CAUTION: Intended recipients should NOT use email communication for emergent or urgent health care matters. >?? >??_______________________________________________ >??Histonet mailing list >??Histonet@lists.utsouthwestern.edu >??http://lists.utsouthwestern.edu/mailman/listinfo/histonet >?? From PAMarcum <@t> uams.edu Thu Jul 24 13:38:09 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Jul 24 13:38:22 2014 Subject: [Histonet] problems Sllde Mate print In-Reply-To: <0B8979A204680A42B93A52B486088CD93938636A02@CUAEXH1.GCU-MD.local> References: <001501cfa76a$1b680e90$52382bb0$@gmx.at> <0B8979A204680A42B93A52B486088CD93938636A02@CUAEXH1.GCU-MD.local> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA320124041C10@Mail2Node2.ad.uams.edu> Same here for printing staying on. We use Thermo ColorFrost, slides have no problems. It may depend on the finish the manufacturer uses for the paint on the slides. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Thursday, July 24, 2014 1:27 PM To: Gudrun Lang; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] problems Sllde Mate print Gudrun, We have 2 slide mates and we run our slides through all reagents and do not have the issue you describe. The only issue we experienced was poor print quality due to the type of paint on the frosted end of the slides. I recently switched to Leica's charged slide that is designed for Thermal Printers like the slide mate and the print quality is top notch and does not fade, peel, or smear when touched and ran through xylene, alcohol, retrieval solutions etc.... Part # 3800090 SLIDE WHITE Xtra Thermal Are your ribbons old? Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.lang@gmx.at] Sent: Thursday, July 24, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problems Sllde Mate print Dear histonetters, we have problems with the stability of the print of our slide mates (Thermo). We use regular slides with frosted end. The print usually looks good and stable at the time of printing. But after staining the print comes off like peeled skin. Very easy to remove. We take care, that the print doesn't come in contact with organic solvents (deparaffination reagens = Histo SAV, butyl acetate), but without any logical reason the first line of the print peels off and the second and third line is rather ok. Has anyone seen similar problems and solved them? Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Lynn.O'Donnell <@t> wchn.org Thu Jul 24 13:43:32 2014 From: Lynn.O'Donnell <@t> wchn.org (O'Donnell, Lynn M.) Date: Thu Jul 24 13:43:42 2014 Subject: [Histonet] problems Sllde Mate print In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA320124041C10@Mail2Node2.ad.uams.edu> References: <001501cfa76a$1b680e90$52382bb0$@gmx.at><0B8979A204680A42B93A52B486088CD93938636A02@CUAEXH1.GCU-MD.local> <41D3A1AF6FEF0643BDC89E0516A6EA320124041C10@Mail2Node2.ad.uams.edu> Message-ID: We use Thin Prep slides for our cytology and have had trouble with the slides smearing. Very frustrating. ______________________________ Lynn M. O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-6704 Email: lynn.o'donnell@wchn.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Thursday, July 24, 2014 14:38 To: Walter Benton; Gudrun Lang; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] problems Sllde Mate print Same here for printing staying on. We use Thermo ColorFrost, slides have no problems. It may depend on the finish the manufacturer uses for the paint on the slides. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Thursday, July 24, 2014 1:27 PM To: Gudrun Lang; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] problems Sllde Mate print Gudrun, We have 2 slide mates and we run our slides through all reagents and do not have the issue you describe. The only issue we experienced was poor print quality due to the type of paint on the frosted end of the slides. I recently switched to Leica's charged slide that is designed for Thermal Printers like the slide mate and the print quality is top notch and does not fade, peel, or smear when touched and ran through xylene, alcohol, retrieval solutions etc.... Part # 3800090 SLIDE WHITE Xtra Thermal Are your ribbons old? Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.lang@gmx.at] Sent: Thursday, July 24, 2014 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] problems Sllde Mate print Dear histonetters, we have problems with the stability of the print of our slide mates (Thermo). We use regular slides with frosted end. The print usually looks good and stable at the time of printing. But after staining the print comes off like peeled skin. Very easy to remove. We take care, that the print doesn't come in contact with organic solvents (deparaffination reagens = Histo SAV, butyl acetate), but without any logical reason the first line of the print peels off and the second and third line is rather ok. Has anyone seen similar problems and solved them? Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brandihisto <@t> hotmail.com Thu Jul 24 14:20:39 2014 From: brandihisto <@t> hotmail.com (Brandi) Date: Thu Jul 24 14:20:43 2014 Subject: [Histonet] Automated stainer for H&E and Pap Message-ID: I am shopping for a new automated stainer and would prefer one that I can do my H&E stain as well as my PAP (GY AND NON-GY) stains on. I know that I'm asking for a lot and will have to purchase extra containers to switch back and forth. I'm most interested in the Leica 5020 & Gemini. I would greatly appreciate any feedback on these two stainers and if anyone else is utilizing either stainer for both stains. Thanks, Brandi Farris From suetp918 <@t> comcast.net Thu Jul 24 14:43:16 2014 From: suetp918 <@t> comcast.net (Sue) Date: Thu Jul 24 14:43:50 2014 Subject: [Histonet] Automated stainer for H&E and Pap In-Reply-To: References: Message-ID: <1EB67D77-EDE9-46AC-8F88-31A759D3AC57@comcast.net> Actually u would have too have 3 set ups since none can share anything. Sent from my iPhone > On Jul 24, 2014, at 3:20 PM, Brandi wrote: > > I am shopping for a new automated stainer and would prefer one that I can do my H&E stain as well as my PAP (GY AND NON-GY) stains on. I know that I'm asking for a lot and will have to purchase extra containers to switch back and forth. I'm most interested in the Leica 5020 & Gemini. I would greatly appreciate any feedback on these two stainers and if anyone else is utilizing either stainer for both stains. > > Thanks, > Brandi Farris > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lori.garcia <@t> medtronic.com Thu Jul 24 15:49:57 2014 From: lori.garcia <@t> medtronic.com (Garcia, Lori, M.Sc.) Date: Thu Jul 24 15:50:07 2014 Subject: [Histonet] Automated stainer for H&E and Pap In-Reply-To: References: Message-ID: <8214022B32A9DE4985A37EA7AE814DA72D3CDA@MSPM1BMSGM41.ent.core.medtronic.com> Hi Brandi, We don't do the PAP stain, but do have multiple stains programmed into our Leica ST5020. It can easily handle both stain programs with switching out containers. Regards, Lori -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Sent: Thursday, July 24, 2014 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated stainer for H&E and Pap I am shopping for a new automated stainer and would prefer one that I can do my H&E stain as well as my PAP (GY AND NON-GY) stains on. I know that I'm asking for a lot and will have to purchase extra containers to switch back and forth. I'm most interested in the Leica 5020 & Gemini. I would greatly appreciate any feedback on these two stainers and if anyone else is utilizing either stainer for both stains. Thanks, Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From JMacDonald <@t> mtsac.edu Fri Jul 25 01:01:39 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jul 25 01:01:49 2014 Subject: [Histonet] Xylene Substitute Protocols In-Reply-To: Message-ID: We have found that Permount works best with the aliphatic hydrocarbons. Sent from my iPad > On Jul 24, 2014, at 10:01 AM, "Hilliard, Dawud Deshawn" wrote: > > Hello all, > > Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success. > > I greatly appreciate the insight! > > Dawud Hilliard > MPM, HTL (ASCP) QIHC > Facility Director > Animal Histopathology Core Lab > Lineberger Comprehensive Cancer Center > University of North Carolina - Chapel Hill > Chapel Hill, North Carolina 27599 > (919) 966-3653 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.O'Donnell <@t> wchn.org Fri Jul 25 05:53:35 2014 From: Lynn.O'Donnell <@t> wchn.org (O'Donnell, Lynn M.) Date: Fri Jul 25 05:53:45 2014 Subject: [Histonet] Automated stainer for H&E and Pap In-Reply-To: References: Message-ID: We use the Leica ST5020 here in our cytology lab. We have 3 staining setups. One Pap, one Nons/FNA, one rush Nons/FNA. And the Nons/FNA and rush Nons/FNA share some dishes. It would not be possible to add an H&E to this machine. And as previously mentioned, the GYN and Nons/FNA have to be separate. A lot of work to change out dishes and increased risk of error. ______________________________ Lynn M. O'Donnell, CT (ASCP), MHA Technical Specialist, Cytology Danbury Hospital 203-739-6704 Email: lynn.o'donnell@wchn.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Sent: Thursday, July 24, 2014 15:21 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated stainer for H&E and Pap I am shopping for a new automated stainer and would prefer one that I can do my H&E stain as well as my PAP (GY AND NON-GY) stains on. I know that I'm asking for a lot and will have to purchase extra containers to switch back and forth. I'm most interested in the Leica 5020 & Gemini. I would greatly appreciate any feedback on these two stainers and if anyone else is utilizing either stainer for both stains. Thanks, Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmai <@t> phenopath.com Fri Jul 25 08:47:04 2014 From: tmai <@t> phenopath.com (Tommy Mai) Date: Fri Jul 25 08:47:14 2014 Subject: [Histonet] Coverslip Message-ID: <53048254-2665-4BD6-9F6A-667E7419D8AA@phenopath.com> Hey everyone, I was wondering if anyone else has seen this occur in their labs and how to resolve this matter. It has to do with the coverslip using the resin tape. However more recently, I have been noticing that there is a drying artifact that happens right as the xylene drys. It looks almost like when water freezes and gives that frosted look. Thanks, Tommy This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mjones <@t> metropath.com Fri Jul 25 09:03:11 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Fri Jul 25 09:03:21 2014 Subject: [Histonet] Coverslip In-Reply-To: <53048254-2665-4BD6-9F6A-667E7419D8AA@phenopath.com> References: <53048254-2665-4BD6-9F6A-667E7419D8AA@phenopath.com> Message-ID: We have found this to be a culmination of a couple of things: Bad lot of tape from vendor Drying slides before cover slipping Too much xylene drip during coverslip (dilutes the glue stuff on the tape allowing for weak adhesive) We use Sakura cover slipping tape for more consistent quality and adjust xylene drip accordingly and make sure slides do not sit out very long at all as xylene from the stainer will evaporate (drying slides) rather quickly. Hope this helps. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 7/25/14, 7:47 AM, "Tommy Mai" wrote: >Hey everyone, > >I was wondering if anyone else has seen this occur in their labs and how >to resolve this matter. It has to do with the coverslip using the resin >tape. However more recently, I have been noticing that there is a drying >artifact that happens right as the xylene drys. It looks almost like >when water freezes and gives that frosted look. > >Thanks, >Tommy > >This e-mail message, including any attachments, is for the sole use of >the >intended recipients and may contain privileged information. Any >unauthorized >review, use, disclosure or distribution is prohibited. If you are not the >intended >recipient, please contact the sender by e-mail and destroy all copies of >the >original message, or you may call PhenoPath Laboratories, Seattle, WA >U.S.A. >at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos <@t> allergydermatology.com Fri Jul 25 09:32:38 2014 From: vavalos <@t> allergydermatology.com (Vanessa Avalos) Date: Fri Jul 25 09:33:01 2014 Subject: [Histonet] RE: Xylene Substitute Protocols In-Reply-To: References: Message-ID: <1dd838aa6c294f90b2b44212ac389a66@BY2PR07MB217.namprd07.prod.outlook.com> Statlab XS-3 & Acrymount. I do H & E staining only. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hilliard, Dawud Deshawn Sent: Thursday, July 24, 2014 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitute Protocols Hello all, Would anyone mind sharing their xylene substitute protocols for their automatic staining, to include the mountant type for coverslipping? We have tried a few, but without much success. We are currently trying Leica's Sub-X, also without much success. I greatly appreciate the insight! Dawud Hilliard MPM, HTL (ASCP) QIHC Facility Director Animal Histopathology Core Lab Lineberger Comprehensive Cancer Center University of North Carolina - Chapel Hill Chapel Hill, North Carolina 27599 (919) 966-3653 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bburnett <@t> CapeCodHealth.org Fri Jul 25 09:35:45 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Fri Jul 25 09:35:52 2014 Subject: [Histonet] Calponin Message-ID: Hi All, I was wondering what stain protocols are being used for Calponin to minimize cross-reactivity with myofibroblasts? We are using a Dako concentrate antibody. I recently re-optimized the stain using a 1:100 dilution and changed the pre-treatment from low pH HIER, to Protease only. This helped a lot with cross-reactivity with myofibroblasts that we were seeing, however we are still seeing some non-specific staining from time to time. Any information would be greatly appreciated! Thanks, Brandy Burnett Cape Cod Hospital ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From Fawn.Bomar <@t> HalifaxRegional.com Fri Jul 25 11:22:59 2014 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Fri Jul 25 11:23:04 2014 Subject: [Histonet] possible TB case Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B974512F@EXCH-2K10.hrhs.com> Hey histonetters!! We just performed a frozen section on a lymph node that looks like it may have TB- I know we will have to decontaminate the cryostat but what else do I have to decontaminate? Will I have to do anything special to the processor or microtome? Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From rjbuesa <@t> yahoo.com Fri Jul 25 11:37:03 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 25 11:37:12 2014 Subject: [Histonet] Automated stainer for H&E and Pap In-Reply-To: References: Message-ID: <1406306223.34728.YahooMailNeo@web120406.mail.ne1.yahoo.com> Contact Sakura Ren? J.? On Thursday, July 24, 2014 3:21 PM, Brandi wrote: I am shopping for a new automated stainer and would prefer one that I can do my H&E stain as well as my PAP (GY AND NON-GY) stains on. I know that I'm asking for a lot and will have to purchase extra containers to switch back and forth. I'm most interested in the Leica 5020 & Gemini. I would greatly appreciate any feedback on these two stainers and if anyone else is utilizing either stainer for both stains. Thanks, Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jul 25 11:40:32 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 25 11:40:43 2014 Subject: [Histonet] Coverslip In-Reply-To: <53048254-2665-4BD6-9F6A-667E7419D8AA@phenopath.com> References: <53048254-2665-4BD6-9F6A-667E7419D8AA@phenopath.com> Message-ID: <1406306432.53728.YahooMailNeo@web120404.mail.ne1.yahoo.com> Usually these odd results have 2 origins that you should look into: 1- using a different (cheaper and harder) plastic than the?originally recommended; and 2- a regulation problem in the amount of xylene dropped into the film Ren? J.? On Friday, July 25, 2014 9:47 AM, Tommy Mai wrote: Hey everyone, I was wondering if anyone else has seen this occur in their labs and how to resolve this matter.? It has to do with the coverslip using the resin tape.? However more recently, I have been noticing that there is a drying artifact that happens right as the xylene drys.? It looks almost like when water freezes and gives that frosted look.? Thanks, Tommy This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Debra.Ortiz <@t> advocatehealth.com Fri Jul 25 11:56:03 2014 From: Debra.Ortiz <@t> advocatehealth.com (Ortiz, Debra) Date: Fri Jul 25 11:56:18 2014 Subject: [Histonet] multitissue control block Message-ID: <82c1870530af42c5ab35d61166352181@BLUPR07MB083.namprd07.prod.outlook.com> Hello, Could someone provide some information on the multi-tissue control block used for IHC? Does anyone have one that is also used for ER/PR too. Thank you Debra Ortiz Technical Director, AP ACL Laboratories, Il Debra.ortiz@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. From craigak12 <@t> gmail.com Fri Jul 25 12:06:42 2014 From: craigak12 <@t> gmail.com (Jb) Date: Fri Jul 25 12:06:48 2014 Subject: [Histonet] IHC Validation: Message-ID: <4FA563B0-5E93-45A8-879F-037B1E1037E4@gmail.com> Does anyone know the preferred method for IHC validation? Is making a tissue microarray of different known cases the preferred method? Does anyone have experience validating like this? Please let me know if anyone has information/tips to doing so. Thank you. Sent from my iPhone From oneilb <@t> wvuhealthcare.com Fri Jul 25 12:32:11 2014 From: oneilb <@t> wvuhealthcare.com (O'neil, Beth) Date: Fri Jul 25 12:32:19 2014 Subject: [Histonet] Slide Mate Print Quality Message-ID: <3CEB8EBCF9C7A648B9694B5696462A714018D582@NT-EXMB1.wvuh.wvuhs.com> In answer to the question about the Slide Mate printing coming off of the slides, we had the same issue about a year ago. At first Thermo though it was the ribbon but it turned out the problem was with the slides. We use the Shandon Superfrost Plus (Cat# 6776214). We contacted Thermo/Shandon and they sent us a different lot. We had to go through about three different lots before the problem was resolved. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC oneilb@wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 From barbara.tibbs <@t> accuratediagnosticlabs.com Fri Jul 25 13:37:17 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Fri Jul 25 13:37:27 2014 Subject: [Histonet] RE: multitissue control block In-Reply-To: <82c1870530af42c5ab35d61166352181@BLUPR07MB083.namprd07.prod.outlook.com> References: <82c1870530af42c5ab35d61166352181@BLUPR07MB083.namprd07.prod.outlook.com> Message-ID: <1406313405883.85175@accuratediagnosticlabs.com> What is it that you want to know? I used to make frozen multiple tissue control blocks for ER/PR many years ago before these antibodies could be used for FFPE tissues. You could do the same thing with paraffin embedded tissue. I've made multi-tissue blocks using a skin biopsy punch tool to obtain small amounts of paraffin-embedded tissues to be used in the control block. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ortiz, Debra Sent: Friday, July 25, 2014 3:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] multitissue control block Hello, Could someone provide some information on the multi-tissue control block used for IHC? Does anyone have one that is also used for ER/PR too. Thank you Debra Ortiz Technical Director, AP ACL Laboratories, Il Debra.ortiz@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Fri Jul 25 14:45:00 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Fri Jul 25 14:45:08 2014 Subject: [Histonet] RE: multitissue control block In-Reply-To: <1406313405883.85175@accuratediagnosticlabs.com> References: <82c1870530af42c5ab35d61166352181@BLUPR07MB083.namprd07.prod.outlook.com> <1406313405883.85175@accuratediagnosticlabs.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F679E7828D@GHSEXMBX1W8K1V.geisinger.edu> We used to use a pc. Of proliferative endometrium, a pc of secretory emdometrium and a pc of cervix in a multitissue block for our ER/PgR control. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Friday, July 25, 2014 2:37 PM To: Ortiz, Debra; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: multitissue control block What is it that you want to know? I used to make frozen multiple tissue control blocks for ER/PR many years ago before these antibodies could be used for FFPE tissues. You could do the same thing with paraffin embedded tissue. I've made multi-tissue blocks using a skin biopsy punch tool to obtain small amounts of paraffin-embedded tissues to be used in the control block. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ortiz, Debra Sent: Friday, July 25, 2014 3:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] multitissue control block Hello, Could someone provide some information on the multi-tissue control block used for IHC? Does anyone have one that is also used for ER/PR too. Thank you Debra Ortiz Technical Director, AP ACL Laboratories, Il Debra.ortiz@advocatehealth.com This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From aneeshdhiman <@t> gmail.com Fri Jul 25 15:21:22 2014 From: aneeshdhiman <@t> gmail.com (Aneesh Dhiman) Date: Fri Jul 25 15:21:26 2014 Subject: [Histonet] (no subject) Message-ID: Hi guys, I need to settle on a coverslip machine, I wonder which is most reliable need 5 peoples at minimum or 10 opinions for the recommendations please Aneesh Dhiman University of Alberta hospital From rjbuesa <@t> yahoo.com Sat Jul 26 10:31:49 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 26 10:34:53 2014 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <1406388709.87809.YahooMailNeo@web120405.mail.ne1.yahoo.com> Your request is quite odd. Are you looking for 5 "guys" to recommend or are you looking for a good coverslipper? This is not an issue of obtaining 5 "guys" with opinions; what if the 5 "guys" advise you to buy the worst coverslipper? I think you need to know the quality of the instrument first. You should contact manufacturers and read specifications. Later you may select 2 and submit those 2 to find out which of the two, according with the histonet "guys" is preferable. I prefer Sakura. There you have it: the opinion of one "guy" Ren? J. On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman wrote: Hi guys,? I need to settle on a coverslip machine, I wonder which is most reliable need 5 peoples at minimum or 10 opinions for the recommendations please Aneesh Dhiman University of Alberta hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Sat Jul 26 11:59:03 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Sat Jul 26 11:59:17 2014 Subject: [Histonet] (no subject) In-Reply-To: <1406388709.87809.YahooMailNeo@web120405.mail.ne1.yahoo.com> References: , <1406388709.87809.YahooMailNeo@web120405.mail.ne1.yahoo.com> Message-ID: I agree. The Sakura tape coverslippers are work horses and will not disappoint. Stick with Sakura's brand of tape as well. There are other brands of tape available, but we found them to be of terrible quality. Brian Cooper CHLA Histology Supervisor -----Original Message----- From: Rene J Buesa [rjbuesa@yahoo.com] Received: Saturday, 26 Jul 2014, 8:38AM To: Aneesh Dhiman [aneeshdhiman@gmail.com]; histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu] Subject: Re: [Histonet] (no subject) Your request is quite odd. Are you looking for 5 "guys" to recommend or are you looking for a good coverslipper? This is not an issue of obtaining 5 "guys" with opinions; what if the 5 "guys" advise you to buy the worst coverslipper? I think you need to know the quality of the instrument first. You should contact manufacturers and read specifications. Later you may select 2 and submit those 2 to find out which of the two, according with the histonet "guys" is preferable. I prefer Sakura. There you have it: the opinion of one "guy" Ren? J. On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman wrote: Hi guys, I need to settle on a coverslip machine, I wonder which is most reliable need 5 peoples at minimum or 10 opinions for the recommendations please Aneesh Dhiman University of Alberta hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From mikeykkk_3 <@t> live.com Sat Jul 26 14:56:27 2014 From: mikeykkk_3 <@t> live.com (Michael Backhus) Date: Sat Jul 26 14:56:33 2014 Subject: [Histonet] Coverslipper Message-ID: The Sakura coverslipper is amazing for any lab or a high volume lab. It coverslips one rack of slides in about 15 seconds. If the coverslip needs to be removed it is very easy. The Leica Biovision coverslipper takes about 5 minutes to coverslip one rack and 5 minutes for the rack to dry. The mounting medium is extremely strong. It can dispense too much on the slide. It's nearly impossible to take the coverslip off. The slides break a lot and it jams often. However I know the Sakura works with Xylene and the Leica Biovision works with Xylene substitute. I'm not sure if they are compatible with either or. Thanks. Mike Sent from my iPhone From jqb7 <@t> cdc.gov Sun Jul 27 19:03:09 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Sun Jul 27 19:03:27 2014 Subject: [Histonet] (no subject) In-Reply-To: <1406388709.87809.YahooMailNeo@web120405.mail.ne1.yahoo.com> References: <1406388709.87809.YahooMailNeo@web120405.mail.ne1.yahoo.com> Message-ID: <3B2CD438E1628A41BD687E98B963B7811FDD7351@EMBX-CHAM2.cdc.gov> not odd at all.........does it matter if it is a glass coverslipper or film? We have a Sakura glass coverslipper and a Leica glass coverslipper. Both have been great. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, July 26, 2014 11:32 AM To: Aneesh Dhiman; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Your request is quite odd. Are you looking for 5 "guys" to recommend or are you looking for a good coverslipper? This is not an issue of obtaining 5 "guys" with opinions; what if the 5 "guys" advise you to buy the worst coverslipper? I think you need to know the quality of the instrument first. You should contact manufacturers and read specifications. Later you may select 2 and submit those 2 to find out which of the two, according with the histonet "guys" is preferable. I prefer Sakura. There you have it: the opinion of one "guy" Ren? J. On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman wrote: Hi guys,? I need to settle on a coverslip machine, I wonder which is most reliable need 5 peoples at minimum or 10 opinions for the recommendations please Aneesh Dhiman University of Alberta hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From miriam.kravitz <@t> gmail.com Sun Jul 27 20:22:23 2014 From: miriam.kravitz <@t> gmail.com (Miriam Kravitz) Date: Sun Jul 27 20:23:08 2014 Subject: [Histonet] Mohs histotech wanted Message-ID: Small private dermatology practice in Cape Cod, Massachusetts, is seeking an experienced Mohs histotech. Salary commensurate with experience. Contact Eric @ 508-2371312 or eric.roth1@comcast.net *Miriam Kravitz, DNP, FNP-BC* Orleans Dermatology & Laser Therapies 3 Namskaket Road Orleans, MA 02653 P: 508-255-4050 F: 888-448-6765 From rmire <@t> cvpath.org Mon Jul 28 06:23:50 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Mon Jul 28 06:24:01 2014 Subject: [Histonet] Request for copy of old Technicon operation manual In-Reply-To: <000001cfa36b$5a8a6080$0f9f2180$@bresnan.net> References: <000001cfa36b$5a8a6080$0f9f2180$@bresnan.net> Message-ID: You could try Docstoc if you can't find a copy anywhere else On Jul 19, 2014, at 12:06 PM, "gayle callis" wrote: > Dear All, > > > > A friend is donating her old Technicon to be used in another country, and > needs a copy of the operation manual. If anyone still has this, could they > send a scanned pdf copy to me asap. I will forward it to person in need > > > > Thank you in advance > > > > Gayle Callis > > HTL/HT/MT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.LaFriniere <@t> ccplab.com Mon Jul 28 07:12:10 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Mon Jul 28 07:12:28 2014 Subject: [Histonet] (no subject) In-Reply-To: <1406388709.87809.YahooMailNeo@web120405.mail.ne1.yahoo.com> References: <1406388709.87809.YahooMailNeo@web120405.mail.ne1.yahoo.com> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2969B351@AHCMSASEXCH00.my.ahc.local> My favorite coverslipper at this time is Leica due to needing glass cover slipping for imaging. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A . Silver Spring, MD 20904 P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, July 26, 2014 11:32 AM To: Aneesh Dhiman; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Your request is quite odd. Are you looking for 5 "guys" to recommend or are you looking for a good coverslipper? This is not an issue of obtaining 5 "guys" with opinions; what if the 5 "guys" advise you to buy the worst coverslipper? I think you need to know the quality of the instrument first. You should contact manufacturers and read specifications. Later you may select 2 and submit those 2 to find out which of the two, according with the histonet "guys" is preferable. I prefer Sakura. There you have it: the opinion of one "guy" Ren? J. On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman wrote: Hi guys,? I need to settle on a coverslip machine, I wonder which is most reliable need 5 peoples at minimum or 10 opinions for the recommendations please Aneesh Dhiman University of Alberta hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Mon Jul 28 08:47:38 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jul 28 08:47:47 2014 Subject: [Histonet] Peggy Wenk's passing Message-ID: It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary?s-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) From PAMarcum <@t> uams.edu Mon Jul 28 09:34:01 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Jul 28 09:34:06 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA3201240422D7@Mail2Node2.ad.uams.edu> I am sorry to hear of our loss in the Histology world and more importantly of a truly good person in our lives. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, July 28, 2014 8:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenk's passing It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary's-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tina.vanmeter <@t> gmail.com Mon Jul 28 09:47:07 2014 From: tina.vanmeter <@t> gmail.com (Tina Van Meter) Date: Mon Jul 28 09:47:16 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA3201240422D7@Mail2Node2.ad.uams.edu> References: <41D3A1AF6FEF0643BDC89E0516A6EA3201240422D7@Mail2Node2.ad.uams.edu> Message-ID: I totally agree with Pam. I can only hope to leave a fraction of an impact when I depart this earth on my colleagues, friends and family as Peggy has left with us. She earned her wings a long time ago and is now at peace. Tina Van Meter On Mon, Jul 28, 2014 at 10:34 AM, Marcum, Pamela A wrote: > I am sorry to hear of our loss in the Histology world and more importantly of a truly good person in our lives. > > Pam Marcum > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > Sent: Monday, July 28, 2014 8:48 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Peggy Wenk's passing > > It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. > > As you know Peggy had a large influence in the world of Histology. > > She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. > She sang enthusiastically in the choir and played in the newly formed bell choir. > > Because of our love of books, we both volunteered at the local library. > We helped collect books, sort them and then helped to sell them; raising funds for the library's use. > > There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. > > St. Mary's-in-the-Hills Episcopal Church (Peggy's church) > > Shades of Pink Foundation (financial aid to local breast cancer patients) > > Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University > > Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). > > Thanks > Lee Wenk (Mr. Peggy Wenk;) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Mon Jul 28 09:57:18 2014 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Jul 28 09:57:30 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA3201240422D7@Mail2Node2.ad.uams.edu> Message-ID: So very sorry to hear of Peggy's passing. She was such as inspiration to us all. She will be greatly missed. May God grant her family the peace and grace to pass into the following days with strength. She will now be soaring with the eagles watching over everyone. Rest in peace Peggy, Colleen Forster On Mon, Jul 28, 2014 at 9:47 AM, Tina Van Meter wrote: > I totally agree with Pam. I can only hope to leave a fraction of an > impact when I depart this earth on my colleagues, friends and family > as Peggy has left with us. She earned her wings a long time ago and is > now at peace. > > Tina Van Meter > > On Mon, Jul 28, 2014 at 10:34 AM, Marcum, Pamela A > wrote: > > I am sorry to hear of our loss in the Histology world and more > importantly of a truly good person in our lives. > > > > Pam Marcum > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > > Sent: Monday, July 28, 2014 8:48 AM > > To: Histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Peggy Wenk's passing > > > > It is my sad duty to tell you that Peggy Wenk passed away peacefully > Saturday morning. Her husband and companion of 37 years and her sister > (from Bend,OR) were there. > > > > As you know Peggy had a large influence in the world of Histology. > > > > She also put herself into her church serving over the years as a nursery > school director, Lay Eucharistic Minister and on the vestry. > > She sang enthusiastically in the choir and played in the newly formed > bell choir. > > > > Because of our love of books, we both volunteered at the local library. > > We helped collect books, sort them and then helped to sell them; raising > funds for the library's use. > > > > There will be two memorial services: one here in Michigan for all her > local family and coworkers, the second in southern California (a burial at > sea). We are asking that no flowers be sent; instead Peggy has specified > (she and her sister planned the funeral several months ago) three different > charitable organizations in lieu of flowers. > > > > St. Mary's-in-the-Hills Episcopal Church (Peggy's church) > > > > Shades of Pink Foundation (financial aid to local breast cancer patients) > > > > Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland > University > > > > Please respond to this email if you'd like more information on the > services or on giving (or to lee@lpwenk.net). > > > > Thanks > > Lee Wenk (Mr. Peggy Wenk;) > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ---------------------------------------------------------------------- > > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential and privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all copies > of the original message. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dmlaud <@t> laudierhistology.com Mon Jul 28 10:10:30 2014 From: dmlaud <@t> laudierhistology.com (Damien) Date: Mon Jul 28 10:10:35 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA3201240422D7@Mail2Node2.ad.uams.edu> Message-ID: Very sorry to read this. Peggy was truly a wonderful person and will be missed. -Damien L. On Mon, Jul 28, 2014 at 10:57 AM, Colleen Forster wrote: > So very sorry to hear of Peggy's passing. She was such as inspiration to us > all. She will be greatly missed. May God grant her family the peace and > grace to pass into the following days with strength. > > She will now be soaring with the eagles watching over everyone. > > Rest in peace Peggy, > > Colleen Forster > > > On Mon, Jul 28, 2014 at 9:47 AM, Tina Van Meter > wrote: > > > I totally agree with Pam. I can only hope to leave a fraction of an > > impact when I depart this earth on my colleagues, friends and family > > as Peggy has left with us. She earned her wings a long time ago and is > > now at peace. > > > > Tina Van Meter > > > > On Mon, Jul 28, 2014 at 10:34 AM, Marcum, Pamela A > > wrote: > > > I am sorry to hear of our loss in the Histology world and more > > importantly of a truly good person in our lives. > > > > > > Pam Marcum > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk > > > Sent: Monday, July 28, 2014 8:48 AM > > > To: Histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Peggy Wenk's passing > > > > > > It is my sad duty to tell you that Peggy Wenk passed away peacefully > > Saturday morning. Her husband and companion of 37 years and her sister > > (from Bend,OR) were there. > > > > > > As you know Peggy had a large influence in the world of Histology. > > > > > > She also put herself into her church serving over the years as a > nursery > > school director, Lay Eucharistic Minister and on the vestry. > > > She sang enthusiastically in the choir and played in the newly formed > > bell choir. > > > > > > Because of our love of books, we both volunteered at the local library. > > > We helped collect books, sort them and then helped to sell them; > raising > > funds for the library's use. > > > > > > There will be two memorial services: one here in Michigan for all her > > local family and coworkers, the second in southern California (a burial > at > > sea). We are asking that no flowers be sent; instead Peggy has specified > > (she and her sister planned the funeral several months ago) three > different > > charitable organizations in lieu of flowers. > > > > > > St. Mary's-in-the-Hills Episcopal Church (Peggy's church) > > > > > > Shades of Pink Foundation (financial aid to local breast cancer > patients) > > > > > > Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland > > University > > > > > > Please respond to this email if you'd like more information on the > > services or on giving (or to lee@lpwenk.net). > > > > > > Thanks > > > Lee Wenk (Mr. Peggy Wenk;) > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ---------------------------------------------------------------------- > > > Confidentiality Notice: This e-mail message, including any attachments, > > is for the sole use of the intended recipient(s) and may contain > > confidential and privileged information. Any unauthorized review, use, > > disclosure or distribution is prohibited. If you are not the intended > > recipient, please contact the sender by reply e-mail and destroy all > copies > > of the original message. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Damien Laudier Laudier Histology www.LaudierHistology.com From akelley <@t> path.wustl.edu Mon Jul 28 10:19:59 2014 From: akelley <@t> path.wustl.edu (Kelley, Amanda) Date: Mon Jul 28 10:20:07 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA3201240422D7@Mail2Node2.ad.uams.edu> Message-ID: Very sorry to hear about Peggy Wenk, she was a wonderful person, always willing to help anybody. She was a great teacher, mentor and friend. She will be missed. She will definitely hear " Well done my good and faithful servant". Because that?s who she was, she was there to help. I can't tell you the number of times I reached out and she was there with an extended hand. She told me last year when she came to visit my booth that she thought this was going to be her last NSH. I told her you don't know that, but I guess she knew better. RIP my dear friend. Amanda Kelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Damien Sent: Monday, July 28, 2014 10:11 AM To: Colleen Forster Cc: Histonet@lists.utsouthwestern.edu; Marcum, Pamela A Subject: Re: [Histonet] Peggy Wenk's passing Very sorry to read this. Peggy was truly a wonderful person and will be missed. -Damien L. On Mon, Jul 28, 2014 at 10:57 AM, Colleen Forster wrote: > So very sorry to hear of Peggy's passing. She was such as inspiration > to us all. She will be greatly missed. May God grant her family the > peace and grace to pass into the following days with strength. > > She will now be soaring with the eagles watching over everyone. > > Rest in peace Peggy, > > Colleen Forster > > > On Mon, Jul 28, 2014 at 9:47 AM, Tina Van Meter > > wrote: > > > I totally agree with Pam. I can only hope to leave a fraction of an > > impact when I depart this earth on my colleagues, friends and family > > as Peggy has left with us. She earned her wings a long time ago and > > is now at peace. > > > > Tina Van Meter > > > > On Mon, Jul 28, 2014 at 10:34 AM, Marcum, Pamela A > > > > wrote: > > > I am sorry to hear of our loss in the Histology world and more > > importantly of a truly good person in our lives. > > > > > > Pam Marcum > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy > > Wenk > > > Sent: Monday, July 28, 2014 8:48 AM > > > To: Histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Peggy Wenk's passing > > > > > > It is my sad duty to tell you that Peggy Wenk passed away > > > peacefully > > Saturday morning. Her husband and companion of 37 years and her > > sister (from Bend,OR) were there. > > > > > > As you know Peggy had a large influence in the world of Histology. > > > > > > She also put herself into her church serving over the years as a > nursery > > school director, Lay Eucharistic Minister and on the vestry. > > > She sang enthusiastically in the choir and played in the newly > > > formed > > bell choir. > > > > > > Because of our love of books, we both volunteered at the local library. > > > We helped collect books, sort them and then helped to sell them; > raising > > funds for the library's use. > > > > > > There will be two memorial services: one here in Michigan for all > > > her > > local family and coworkers, the second in southern California (a > > burial > at > > sea). We are asking that no flowers be sent; instead Peggy has > > specified (she and her sister planned the funeral several months > > ago) three > different > > charitable organizations in lieu of flowers. > > > > > > St. Mary's-in-the-Hills Episcopal Church (Peggy's church) > > > > > > Shades of Pink Foundation (financial aid to local breast cancer > patients) > > > > > > Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland > > University > > > > > > Please respond to this email if you'd like more information on the > > services or on giving (or to lee@lpwenk.net). > > > > > > Thanks > > > Lee Wenk (Mr. Peggy Wenk;) > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------------------------------------------ > > > ---- Confidentiality Notice: This e-mail message, including any > > > attachments, > > is for the sole use of the intended recipient(s) and may contain > > confidential and privileged information. Any unauthorized review, > > use, disclosure or distribution is prohibited. If you are not the > > intended recipient, please contact the sender by reply e-mail and > > destroy all > copies > > of the original message. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Damien Laudier Laudier Histology www.LaudierHistology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From mjones <@t> metropath.com Mon Jul 28 10:21:41 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Jul 28 10:22:02 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: <41D3A1AF6FEF0643BDC89E0516A6EA3201240422D7@Mail2Node2.ad.uams.edu> Message-ID: So sorry to hear of the loss of a wonderful woman. Michael Ann On 7/28/14, 9:10 AM, "Damien" wrote: >Very sorry to read this. Peggy was truly a wonderful person and will be >missed. > >-Damien L. > > >On Mon, Jul 28, 2014 at 10:57 AM, Colleen Forster >wrote: > >> So very sorry to hear of Peggy's passing. She was such as inspiration >>to us >> all. She will be greatly missed. May God grant her family the peace and >> grace to pass into the following days with strength. >> >> She will now be soaring with the eagles watching over everyone. >> >> Rest in peace Peggy, >> >> Colleen Forster >> >> >> On Mon, Jul 28, 2014 at 9:47 AM, Tina Van Meter >> >> wrote: >> >> > I totally agree with Pam. I can only hope to leave a fraction of an >> > impact when I depart this earth on my colleagues, friends and family >> > as Peggy has left with us. She earned her wings a long time ago and is >> > now at peace. >> > >> > Tina Van Meter >> > >> > On Mon, Jul 28, 2014 at 10:34 AM, Marcum, Pamela A >> > wrote: >> > > I am sorry to hear of our loss in the Histology world and more >> > importantly of a truly good person in our lives. >> > > >> > > Pam Marcum >> > > >> > > -----Original Message----- >> > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: >> > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy >>Wenk >> > > Sent: Monday, July 28, 2014 8:48 AM >> > > To: Histonet@lists.utsouthwestern.edu >> > > Subject: [Histonet] Peggy Wenk's passing >> > > >> > > It is my sad duty to tell you that Peggy Wenk passed away peacefully >> > Saturday morning. Her husband and companion of 37 years and her >>sister >> > (from Bend,OR) were there. >> > > >> > > As you know Peggy had a large influence in the world of Histology. >> > > >> > > She also put herself into her church serving over the years as a >> nursery >> > school director, Lay Eucharistic Minister and on the vestry. >> > > She sang enthusiastically in the choir and played in the newly >>formed >> > bell choir. >> > > >> > > Because of our love of books, we both volunteered at the local >>library. >> > > We helped collect books, sort them and then helped to sell them; >> raising >> > funds for the library's use. >> > > >> > > There will be two memorial services: one here in Michigan for all >>her >> > local family and coworkers, the second in southern California (a >>burial >> at >> > sea). We are asking that no flowers be sent; instead Peggy has >>specified >> > (she and her sister planned the funeral several months ago) three >> different >> > charitable organizations in lieu of flowers. >> > > >> > > St. Mary's-in-the-Hills Episcopal Church (Peggy's church) >> > > >> > > Shades of Pink Foundation (financial aid to local breast cancer >> patients) >> > > >> > > Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland >> > University >> > > >> > > Please respond to this email if you'd like more information on the >> > services or on giving (or to lee@lpwenk.net). >> > > >> > > Thanks >> > > Lee Wenk (Mr. Peggy Wenk;) >> > > _______________________________________________ >> > > Histonet mailing list >> > > Histonet@lists.utsouthwestern.edu >> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > >> > > >>---------------------------------------------------------------------- >> > > Confidentiality Notice: This e-mail message, including any >>attachments, >> > is for the sole use of the intended recipient(s) and may contain >> > confidential and privileged information. Any unauthorized review, use, >> > disclosure or distribution is prohibited. If you are not the intended >> > recipient, please contact the sender by reply e-mail and destroy all >> copies >> > of the original message. >> > > >> > > _______________________________________________ >> > > Histonet mailing list >> > > Histonet@lists.utsouthwestern.edu >> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > >-- >Damien Laudier >Laudier Histology >www.LaudierHistology.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Mon Jul 28 10:54:48 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Jul 28 10:54:56 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA3201240422D7@Mail2Node2.ad.uams.edu> References: <41D3A1AF6FEF0643BDC89E0516A6EA3201240422D7@Mail2Node2.ad.uams.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF9D33769@MERCERMAIL.MercerU.local> I agree, she was such a great example of professionalism for the histology community. She will be greatly missed. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Monday, July 28, 2014 10:34 AM To: Lee & Peggy Wenk; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Peggy Wenk's passing I am sorry to hear of our loss in the Histology world and more importantly of a truly good person in our lives. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, July 28, 2014 8:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenk's passing It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary's-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Mon Jul 28 11:37:23 2014 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Mon Jul 28 11:37:35 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: Message-ID: <2E99EE0A-7C30-4303-9CD1-D19226236EC6@gmail.com> I am so so sorry to hear this news. I knew of Peggy's dedication to the field of histology & her knowledge of it. She was a lovely lovely person. My sincere sympathies to you Lee and the rest of Peggy's family and friends. Maria Mejia San Francisco, California On Jul 28, 2014, at 6:47 AM, Lee & Peggy Wenk wrote: > It is my sad duty to tell you that Peggy Wenk passed away peacefully > Saturday morning. Her husband and companion of 37 years and her > sister (from Bend,OR) were there. > > As you know Peggy had a large influence in the world of Histology. > > She also put herself into her church serving over the years as > a nursery school director, Lay Eucharistic Minister and on the vestry. > She sang enthusiastically in the choir and played in the newly formed > bell choir. > > Because of our love of books, we both volunteered at the local library. > We helped collect books, sort them and then helped to sell them; raising > funds for the library's use. > > There will be two memorial services: one here in Michigan for all her > local family and coworkers, the second in southern California (a burial > at sea). We are asking that no flowers be sent; instead Peggy has > specified (she and her sister planned the funeral several months ago) > three different charitable organizations in lieu of flowers. > > St. Mary?s-in-the-Hills Episcopal Church (Peggy's church) > > Shades of Pink Foundation (financial aid to local breast cancer patients) > > Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University > > Please respond to this email if you'd like more information on the > services or on giving (or to lee@lpwenk.net). > > Thanks > Lee Wenk (Mr. Peggy Wenk;) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Mon Jul 28 11:52:06 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Jul 28 11:52:17 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: Message-ID: Lee, I am so sorry to hear about your loss, and a huge loss and void to the world of histology. My sympathies go out to you and your family. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, July 28, 2014 6:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenk's passing It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary's-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Mon Jul 28 12:32:54 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Mon Jul 28 12:32:58 2014 Subject: [Histonet] Re: Peggy Wenk's passing Message-ID: <9F3CFEE76E51B64991C7485270890B40498F692B@EX5.lj.gnf.org> Dear Mr. Peggy, Thank you for letting us know. It had to be a difficult letter to write, but I am grateful for it. I will address the remainder directly to Peggy. Dear Peggy, Thank you for all you have done to shape the course and future of Histotechnology. Trust that your contributions will not end here, but will continue forever. I can't tell you how many times, when explaining histology to someone, I start with "Peggy Wenk says.." or "look it up on the Histonet, Peggy Wenk has a great explanation for this." We will take up the baton in your honor and keep moving forward. My sincerest condolences to your family, but I am relieved you are at peace. ~Teri From tbraud <@t> holyredeemer.com Mon Jul 28 13:14:10 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Jul 28 13:14:15 2014 Subject: [Histonet] Peggy Wenk's passing Message-ID: I am deeply saddened to hear of this loss. My condolences to her friends and family. Our profession and our world is a lesser place without her. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From renafail2 <@t> gmail.com Mon Jul 28 13:42:01 2014 From: renafail2 <@t> gmail.com (Rena Fail) Date: Mon Jul 28 13:42:09 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: Message-ID: So sorry to hear of Peggy passing may she have peace. At last On Monday, July 28, 2014, Lee & Peggy Wenk wrote: > It is my sad duty to tell you that Peggy Wenk passed away peacefully > Saturday morning. Her husband and companion of 37 years and her > sister (from Bend,OR) were there. > > As you know Peggy had a large influence in the world of Histology. > > She also put herself into her church serving over the years as > a nursery school director, Lay Eucharistic Minister and on the vestry. > She sang enthusiastically in the choir and played in the newly formed > bell choir. > > Because of our love of books, we both volunteered at the local library. > We helped collect books, sort them and then helped to sell them; raising > funds for the library's use. > > There will be two memorial services: one here in Michigan for all her > local family and coworkers, the second in southern California (a burial > at sea). We are asking that no flowers be sent; instead Peggy has > specified (she and her sister planned the funeral several months ago) > three different charitable organizations in lieu of flowers. > > St. Mary?s-in-the-Hills Episcopal Church (Peggy's church) > > Shades of Pink Foundation (financial aid to local breast cancer patients) > > Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University > > Please respond to this email if you'd like more information on the > services or on giving (or to lee@lpwenk.net ). > > Thanks > Lee Wenk (Mr. Peggy Wenk;) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kalschev <@t> svm.vetmed.wisc.edu Mon Jul 28 13:49:03 2014 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Mon Jul 28 13:49:08 2014 Subject: [Histonet] Resend: Peggy Message-ID: <53D69B1F.8030802@svm.vetmed.wisc.edu> Lee and Family, You are not alone in your grief. Peggy enriched our lives. I applaud her example, dedication and humor. Thank you for letting us know. ~ Circle of Life~. Peace, Vicki K -- Vicki L. Kalscheur From gagnone <@t> KGH.KARI.NET Mon Jul 28 14:55:34 2014 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Mon Jul 28 14:55:45 2014 Subject: [Histonet] Peggy Wenk's passing Message-ID: <5F06C3AD0B27264CA20CFA986C87882EC70CC7AD@EXCHANGEPV1.KGH.ON.CA> Peggy's reach in our field went beyond borders. Here and I'm sure in other Canadian sites, we were fortunate to benefit from her enthusiasm and expertise online in forums such as this, as well as through teleconferences and blogging. Such knowledge and years of experience are not easily or quickly replaced. Condolences, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From ltougas <@t> dawsoncollege.qc.ca Mon Jul 28 15:09:49 2014 From: ltougas <@t> dawsoncollege.qc.ca (Liette Tougas) Date: Mon Jul 28 15:10:13 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: Message-ID: <455897B94CF3A44F81F727160F23FB112CE9E628@DC229.ad.dawsoncollege.qc.ca> My sincere condolences to you, your family and Peggy's friends. Our discipline has truly lost an important contributor and a great person. At least she lived to receive the J.B. McCormick Award last year, which was well deserved. Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal 514-931-8731, ext 1519 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Lee & Peggy Wenk [lpwenk@sbcglobal.net] Sent: July 28, 2014 9:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenk's passing It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary?s-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ibernard <@t> uab.edu Mon Jul 28 15:31:10 2014 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Mon Jul 28 15:31:17 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: Message-ID: On behalf of all military histologist who knew Peggy, please accept our condolences. I had the pleasure of attending her NSH seminars and seeking out questions from her as recently as a few months ago. Sorry to hear of her passing. She was a true champion in the field of Histology and now will be a legend. God Bless you and family Ian Bernard USAF Academy, Colorado. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, July 28, 2014 7:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenk's passing It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary's-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Jul 28 18:48:18 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jul 28 18:48:39 2014 Subject: [Histonet] Peggy Wenk's passing In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00F6584@xmdb04.nch.kids> This is so very sad. A great lady who will be missed by the international histotechnology community. A knowledgeable and patient educator. I hope we can live up to her memory and mirror her professionalism in our own careers. Let's make her proud of us. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, 28 July 2014 11:48 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenk's passing It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary's-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From histotalk <@t> yahoo.com Mon Jul 28 20:35:20 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Mon Jul 28 20:38:25 2014 Subject: [Histonet] Peggy Wenk Message-ID: <1406597720.10329.YahooMailNeo@web141106.mail.bf1.yahoo.com> Peggy's last interview on HistoTalk www.histotalk.com?was taped last year at the NSH. During her interview, I stopped taping several times, because the tears and my choking up were too much for me. Each time I stopped, Peggy smiled at me and said 'It's Ok, Dave, it's OK". It's a shame HistoTalk never got the recognition it truly deserved. Interviews like Peggy's have meaning for everyone in the profession. Over the years, HistoTalk has?had a small group of champions - Peggy was one of them! God bless. To "Mr. Peggy Wenk", May the Universal Power be within you and your loved ones, now and always. Dave From christina.kreutzer01 <@t> gmail.com Tue Jul 29 06:26:14 2014 From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer) Date: Tue Jul 29 06:26:24 2014 Subject: [Histonet] Quartzy Message-ID: Hello everybody, we are currently thinking about a system to organize our lab-inventory and orders. Somebody recommended Quartzy to us (https://www.google.at/#q=quartzy+). Now I was wondering if anybody of you is using this system already, and what experiences you were having with it so far. Please share your thaughts. All the best Christina From histotalk <@t> yahoo.com Tue Jul 29 07:08:12 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Jul 29 07:11:14 2014 Subject: [Histonet] Peggy Wenk In-Reply-To: <1406597720.10329.YahooMailNeo@web141106.mail.bf1.yahoo.com> Message-ID: <1406635692.31100.YahooMailBasic@web141106.mail.bf1.yahoo.com> Peggy's last interview on HistoTalk www.histotalk.com?was taped last year at the NSH. During her interview, I stopped taping several times, because the tears and my choking up were too much for me. Each time I stopped, Peggy smiled at me and said 'It's Ok, Dave, it's OK". It's a shame HistoTalk never got the recognition it truly deserved. Interviews like Peggy's have meaning for everyone in the profession. Over the years, HistoTalk has?had a small group of champions - Peggy was one of them! God bless. To "Mr. Peggy Wenk", May the Universal Power be within you and your loved ones, now and always. Dave ? From j.rowaihi <@t> alborglaboratories.com Tue Jul 29 08:24:01 2014 From: j.rowaihi <@t> alborglaboratories.com (j.rowaihi) Date: Tue Jul 29 08:24:19 2014 Subject: =?UTF-8?Q?=D8=B1=D8=AF:_[Histonet]_Quartzy?= Message-ID: I have no experience on it, But it looks very helpful system.? Regards Jamal Rowaihi Sent from my cell phone -------- ??????? ??????? -------- ??: Christina Kreutzer ???????: ????/??/?? ?:?? ? (GMT+03:00) ???: "" ???????: [Histonet] Quartzy Hello everybody, we are currently thinking about a system to organize our lab-inventory and orders. Somebody recommended Quartzy to us (https://www.google.at/#q=quartzy+). Now I was wondering if anybody of you is using this system already, and what experiences you were having with it so far. Please share your thaughts. All the best Christina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Tue Jul 29 08:38:24 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Jul 29 08:38:28 2014 Subject: [Histonet] Peggy Wenk Interview Link Message-ID: <1406641104.99062.YahooMailNeo@web141105.mail.bf1.yahoo.com> The direct link is www.HistoTalk.net Yours, Dave From POWELL_SA <@t> mercer.edu Tue Jul 29 08:53:14 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Tue Jul 29 08:53:36 2014 Subject: [Histonet] Peggy Wenk In-Reply-To: <1406597720.10329.YahooMailNeo@web141106.mail.bf1.yahoo.com> References: <1406597720.10329.YahooMailNeo@web141106.mail.bf1.yahoo.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF9D33C24@MERCERMAIL.MercerU.local> Thanks Dave. Yes she was so brave. Also HistoTalk is a great venue for histotechs all over the world to get to hear great educators and histology professional champions interviewed by you. Younger techs will see their names many times but may never be able to relate to or get to know their good examples set for our profession. But thanks to Histotalk they can go back and listen to what these former ones have accomplished and how they did it. Such an inspiration. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Kemler Sent: Monday, July 28, 2014 9:35 PM To: Histonet Subject: [Histonet] Peggy Wenk Peggy's last interview on HistoTalk www.histotalk.com?was taped last year at the NSH. During her interview, I stopped taping several times, because the tears and my choking up were too much for me. Each time I stopped, Peggy smiled at me and said 'It's Ok, Dave, it's OK". It's a shame HistoTalk never got the recognition it truly deserved. Interviews like Peggy's have meaning for everyone in the profession. Over the years, HistoTalk has?had a small group of champions - Peggy was one of them! God bless. To "Mr. Peggy Wenk", May the Universal Power be within you and your loved ones, now and always. Dave _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Tue Jul 29 09:19:31 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jul 29 09:19:38 2014 Subject: [Histonet] Peggy Wenk In-Reply-To: <1406635692.31100.YahooMailBasic@web141106.mail.bf1.yahoo.com> References: <1406597720.10329.YahooMailNeo@web141106.mail.bf1.yahoo.com> <1406635692.31100.YahooMailBasic@web141106.mail.bf1.yahoo.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F1C55@evcspmbx2.ads.northwestern.edu> This is the announcement on the NSH page: http://nsh.org/content/peggy-wenk-true-mentor She will be missed! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Kemler Sent: Tuesday, July 29, 2014 7:08 AM To: Fellow HistoNetters Subject: [Histonet] Peggy Wenk Peggy's last interview on HistoTalk www.histotalk.com?was taped last year at the NSH. During her interview, I stopped taping several times, because the tears and my choking up were too much for me. Each time I stopped, Peggy smiled at me and said 'It's Ok, Dave, it's OK". It's a shame HistoTalk never got the recognition it truly deserved. Interviews like Peggy's have meaning for everyone in the profession. Over the years, HistoTalk has?had a small group of champions - Peggy was one of them! God bless. To "Mr. Peggy Wenk", May the Universal Power be within you and your loved ones, now and always. Dave ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wsimons <@t> athensgastro.com Tue Jul 29 09:20:09 2014 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Tue Jul 29 09:20:14 2014 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUGVnZ3kgV2Vuaw==?= In-Reply-To: <1406635692.31100.YahooMailBasic@web141106.mail.bf1.yahoo.com> References: <1406635692.31100.YahooMailBasic@web141106.mail.bf1.yahoo.com> Message-ID: <20140729142009.8074.qmail@server276.com> Thank you Dave for your selfless contribution of preserving the legends of Histotechnology. The Georgia Society for Histotechnology supports you and we applaud you for this wonderful gift. Thank you for sharing this precious soul~ Heartfelt love and condolences Wanda K Simons GSH President > -------Original Message------- > From: David Kemler > To: Fellow HistoNetters > Subject: [Histonet] Peggy Wenk > Sent: Jul 29 '14 08:08 > > > Peggy's last interview > on HistoTalk www.histotalk.com?was taped last > year at the NSH. During her interview, I stopped taping > several times, because the tears and my choking up were too > much for me. Each time I stopped, Peggy smiled at me and > said 'It's Ok, Dave, it's OK". > It's > a shame HistoTalk never got the recognition it truly > deserved. Interviews like Peggy's have meaning for > ??everyone in the profession. Over the years, HistoTalk > has?had a > small group of champions - Peggy was one of them! God bless. > To "Mr. Peggy Wenk", May the Universal Power be > within you and your loved ones, now and > always. > Dave > ? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cls71877 <@t> gmail.com Tue Jul 29 13:33:35 2014 From: cls71877 <@t> gmail.com (Cristi Stephenson) Date: Tue Jul 29 13:33:41 2014 Subject: [Histonet] Processor Alarms Message-ID: Hello Histoland, We have an ASP300S processor that is not currently monitored by Leica. I was wondering if anyone had an alarm system suggestion such that, in the event it does malfunction, it will notify a designated person. We are a private practice so there is no one in the building after "normal" working hours. Thanks in advance for any recommendations! Cristi From cls71877 <@t> gmail.com Tue Jul 29 14:56:52 2014 From: cls71877 <@t> gmail.com (Cristi) Date: Tue Jul 29 14:57:12 2014 Subject: [Histonet] Thank you! Message-ID: Thank you all for the responses on alarm systems for the processors. I knew they were out there but couldn't come up with the right combination for Google to understand what I was looking for ? Sent from my iPhone From hfabian <@t> peralta.edu Wed Jul 30 12:32:19 2014 From: hfabian <@t> peralta.edu (Hank Fabian) Date: Wed Jul 30 12:32:29 2014 Subject: [Histonet] Histonet requests Message-ID: I am starting a new Histotech program in Oakland California. I am seeking certified Histotechnicians for instructors. Is there anyway I can post this? Thanks, Hank From llang <@t> aipathology.com Wed Jul 30 13:55:26 2014 From: llang <@t> aipathology.com (LeAnn Lang) Date: Wed Jul 30 13:55:36 2014 Subject: [Histonet] Noise reduction Message-ID: We are trying to figure out an inexpensive way to try to block off our grossing area to help with noise reduction when our Pathologist Assistant does her dictation. Has anyone done anything in their labs that they would be willing to share with me? We don't want something permanent like building a wall, but rather something that can be opened up for parts of the day. We have thought about having a curtain (like a privacy curtain found in doctors' offices), but we are not sure that would be enough of a reduction. Anyone have other ideas? Thanks much! LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com From cls71877 <@t> gmail.com Wed Jul 30 13:57:27 2014 From: cls71877 <@t> gmail.com (Cristi Stephenson) Date: Wed Jul 30 13:57:35 2014 Subject: [Histonet] Some Responses for Tissue Processor Alarms Message-ID: I have had numerous requests to share the responses for the tissue alarm processors. Please see below for some of the answers. Please feel freeto contact if I can be of more help :) Happy Hump Day! Christi We use SensaPhone2000 alarm system for our3 VIP processors. It can accommodate up to 8 instruments. However, it only tells you if there is an error or power failure, not what the error is. You can set up phone call, pager, email and web interface. You do need a dedicated phone line for it, or accept that it will interrupt and grab the existing line if needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA Cristi, I previously used an auto dialer like this one on a VIP. When it alarms it calls three numbers until it receives an answer. It will deliver a pre-recorded message the you will record e.g. tissue processor is broken come fix me. http://www.amazon.com/dp/B005WE9AD2?tag=shoppingstoresco-20 I'm sure there are other auto dialers out there that would work as well. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Hi Cristi, I'm currently in a small lab and my VIP is on a surge protector, but also has an alarm connected to the "after hours" on call folks. I got the system thru my thermo shandon rep. Hope this helps. And of course my IT guy here at the facility did all the connections. Wanda From amber.mckenzie <@t> gastrodocs.net Wed Jul 30 15:13:27 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Jul 30 15:13:33 2014 Subject: [Histonet] Schiff : room temp or not In-Reply-To: References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> Message-ID: <5A33C952BB67F4468AF1F36D739212BC011251D957@JERRY.Gia.com> When staining PAS's, does the Schiff need to be room temp or does it matter? Just curious about when to add it to our Sakura stainer. Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! From Tony.Reilly <@t> health.qld.gov.au Wed Jul 30 17:25:29 2014 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Wed Jul 30 17:26:24 2014 Subject: [Histonet] RE: Noise reduction In-Reply-To: References: Message-ID: Hi LeAnn At a previous laboratory we had a half wall with glass from bench height to the ceiling and a glass door. The door could be open or closed and the glass while blocking out noise did not make the area feel confined from the rest of the lab. The other advantages were that the air conditioning to that room was independent and not recycled to the rest of the lab reducing formalin fumes and if there was a formalin spill in that area it was easily contained. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Princess Alexandra Laboratory | Pathology Queensland | Health Support Queensland Department of Health | Queensland Government Building 15, Level 1,? 199 Ipswich Road? Woolloongabba Qld 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang Sent: Thursday, 31 July 2014 4:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Noise reduction We are trying to figure out an inexpensive way to try to block off our grossing area to help with noise reduction when our Pathologist Assistant does her dictation. Has anyone done anything in their labs that they would be willing to share with me? We don't want something permanent like building a wall, but rather something that can be opened up for parts of the day. We have thought about having a curtain (like a privacy curtain found in doctors' offices), but we are not sure that would be enough of a reduction. Anyone have other ideas? Thanks much! LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From SteveM <@t> mcclainlab.com Wed Jul 30 17:26:01 2014 From: SteveM <@t> mcclainlab.com (Steve McClain) Date: Wed Jul 30 17:26:43 2014 Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 29 In-Reply-To: <72b37e7a-58a0-4ce6-b3cc-8dc46816c4e3@ML1.McClainLabs.local> References: <72b37e7a-58a0-4ce6-b3cc-8dc46816c4e3@ML1.McClainLabs.local> Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DF83BCA4C9@ML1.McClainLabs.local> I requested 15 levels at 200 microns, that gives us 3mm of detail 5 slices per mm. Steve 631 361 4000 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, July 29, 2014 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 128, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Peggy Wenk's passing (Teri Johnson) 2. Peggy Wenk's passing (Terri Braud) 3. Re: Peggy Wenk's passing (Rena Fail) 4. Resend: Peggy (Vicki Kalscheur) 5. Peggy Wenk's passing (Gagnon, Eric) 6. RE: Peggy Wenk's passing (Liette Tougas) 7. RE: Peggy Wenk's passing (Ian R Bernard) 8. RE: Peggy Wenk's passing (Tony Henwood (SCHN)) 9. Peggy Wenk (David Kemler) 10. Quartzy (Christina Kreutzer) 11. Peggy Wenk (David Kemler) 12. ??: [Histonet] Quartzy (j.rowaihi) 13. Peggy Wenk Interview Link (David Kemler) 14. RE: Peggy Wenk (Shirley A. Powell) 15. RE: Peggy Wenk (Bernice Frederick) 16. Re: Peggy Wenk (wsimons@athensgastro.com) ---------------------------------------------------------------------- Message: 1 Date: Mon, 28 Jul 2014 17:32:54 +0000 From: Teri Johnson Subject: [Histonet] Re: Peggy Wenk's passing To: "histonet@lists.utsouthwestern.edu" Message-ID: <9F3CFEE76E51B64991C7485270890B40498F692B@EX5.lj.gnf.org> Content-Type: text/plain; charset="us-ascii" Dear Mr. Peggy, Thank you for letting us know. It had to be a difficult letter to write, but I am grateful for it. I will address the remainder directly to Peggy. Dear Peggy, Thank you for all you have done to shape the course and future of Histotechnology. Trust that your contributions will not end here, but will continue forever. I can't tell you how many times, when explaining histology to someone, I start with "Peggy Wenk says.." or "look it up on the Histonet, Peggy Wenk has a great explanation for this." We will take up the baton in your honor and keep moving forward. My sincerest condolences to your family, but I am relieved you are at peace. ~Teri ------------------------------ Message: 2 Date: Mon, 28 Jul 2014 14:14:10 -0400 From: "Terri Braud" Subject: [Histonet] Peggy Wenk's passing To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am deeply saddened to hear of this loss. My condolences to her friends and family. Our profession and our world is a lesser place without her. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ------------------------------ Message: 3 Date: Mon, 28 Jul 2014 14:42:01 -0400 From: Rena Fail Subject: Re: [Histonet] Peggy Wenk's passing To: "Lee & Peggy Wenk" Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 So sorry to hear of Peggy passing may she have peace. At last On Monday, July 28, 2014, Lee & Peggy Wenk wrote: > It is my sad duty to tell you that Peggy Wenk passed away peacefully > Saturday morning. Her husband and companion of 37 years and her > sister (from Bend,OR) were there. > > As you know Peggy had a large influence in the world of Histology. > > She also put herself into her church serving over the years as a > nursery school director, Lay Eucharistic Minister and on the vestry. > She sang enthusiastically in the choir and played in the newly formed > bell choir. > > Because of our love of books, we both volunteered at the local library. > We helped collect books, sort them and then helped to sell them; > raising funds for the library's use. > > There will be two memorial services: one here in Michigan for all her > local family and coworkers, the second in southern California (a > burial at sea). We are asking that no flowers be sent; instead Peggy > has specified (she and her sister planned the funeral several months > ago) three different charitable organizations in lieu of flowers. > > St. Mary???s-in-the-Hills Episcopal Church (Peggy's church) > > Shades of Pink Foundation (financial aid to local breast cancer > patients) > > Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland > University > > Please respond to this email if you'd like more information on the > services or on giving (or to lee@lpwenk.net ). > > Thanks > Lee Wenk (Mr. Peggy Wenk;) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Mon, 28 Jul 2014 13:49:03 -0500 From: Vicki Kalscheur Subject: [Histonet] Resend: Peggy To: "histonet@lists.utsouthwestern.edu" Message-ID: <53D69B1F.8030802@svm.vetmed.wisc.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Lee and Family, You are not alone in your grief. Peggy enriched our lives. I applaud her example, dedication and humor. Thank you for letting us know. ~ Circle of Life~. Peace, Vicki K -- Vicki L. Kalscheur ------------------------------ Message: 5 Date: Mon, 28 Jul 2014 19:55:34 +0000 From: "Gagnon, Eric" Subject: [Histonet] Peggy Wenk's passing To: "histonet@lists.utsouthwestern.edu" Message-ID: <5F06C3AD0B27264CA20CFA986C87882EC70CC7AD@EXCHANGEPV1.KGH.ON.CA> Content-Type: text/plain; charset="iso-8859-1" Peggy's reach in our field went beyond borders. Here and I'm sure in other Canadian sites, we were fortunate to benefit from her enthusiasm and expertise online in forums such as this, as well as through teleconferences and blogging. Such knowledge and years of experience are not easily or quickly replaced. Condolences, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ------------------------------ Message: 6 Date: Mon, 28 Jul 2014 20:09:49 +0000 From: Liette Tougas Subject: RE: [Histonet] Peggy Wenk's passing To: "Histonet@lists.utsouthwestern.edu" Message-ID: <455897B94CF3A44F81F727160F23FB112CE9E628@DC229.ad.dawsoncollege.qc.ca> Content-Type: text/plain; charset="Windows-1252" My sincere condolences to you, your family and Peggy's friends. Our discipline has truly lost an important contributor and a great person. At least she lived to receive the J.B. McCormick Award last year, which was well deserved. Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal 514-931-8731, ext 1519 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Lee & Peggy Wenk [lpwenk@sbcglobal.net] Sent: July 28, 2014 9:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenk's passing It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary?s-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 28 Jul 2014 20:31:10 +0000 From: Ian R Bernard Subject: RE: [Histonet] Peggy Wenk's passing To: Lee & Peggy Wenk , "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" On behalf of all military histologist who knew Peggy, please accept our condolences. I had the pleasure of attending her NSH seminars and seeking out questions from her as recently as a few months ago. Sorry to hear of her passing. She was a true champion in the field of Histology and now will be a legend. God Bless you and family Ian Bernard USAF Academy, Colorado. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, July 28, 2014 7:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenk's passing It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary's-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 28 Jul 2014 23:48:18 +0000 From: "Tony Henwood (SCHN)" Subject: RE: [Histonet] Peggy Wenk's passing To: "'Lee & Peggy Wenk'" , "Histonet@lists.utsouthwestern.edu" Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00F6584@xmdb04.nch.kids> Content-Type: text/plain; charset="us-ascii" This is so very sad. A great lady who will be missed by the international histotechnology community. A knowledgeable and patient educator. I hope we can live up to her memory and mirror her professionalism in our own careers. Let's make her proud of us. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, 28 July 2014 11:48 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Peggy Wenk's passing It is my sad duty to tell you that Peggy Wenk passed away peacefully Saturday morning. Her husband and companion of 37 years and her sister (from Bend,OR) were there. As you know Peggy had a large influence in the world of Histology. She also put herself into her church serving over the years as a nursery school director, Lay Eucharistic Minister and on the vestry. She sang enthusiastically in the choir and played in the newly formed bell choir. Because of our love of books, we both volunteered at the local library. We helped collect books, sort them and then helped to sell them; raising funds for the library's use. There will be two memorial services: one here in Michigan for all her local family and coworkers, the second in southern California (a burial at sea). We are asking that no flowers be sent; instead Peggy has specified (she and her sister planned the funeral several months ago) three different charitable organizations in lieu of flowers. St. Mary's-in-the-Hills Episcopal Church (Peggy's church) Shades of Pink Foundation (financial aid to local breast cancer patients) Peggy A. Wenk Endowed Scholarship for Histotechnology at Oakland University Please respond to this email if you'd like more information on the services or on giving (or to lee@lpwenk.net). Thanks Lee Wenk (Mr. Peggy Wenk;) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 9 Date: Mon, 28 Jul 2014 18:35:20 -0700 From: David Kemler Subject: [Histonet] Peggy Wenk To: Histonet Message-ID: <1406597720.10329.YahooMailNeo@web141106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Peggy's last interview on HistoTalk www.histotalk.com?was taped last year at the NSH. During her interview, I stopped taping several times, because the tears and my choking up were too much for me. Each time I stopped, Peggy smiled at me and said 'It's Ok, Dave, it's OK". It's a shame HistoTalk never got the recognition it truly deserved. Interviews like Peggy's have meaning for everyone in the profession. Over the years, HistoTalk has?had a small group of champions - Peggy was one of them! God bless. To "Mr. Peggy Wenk", May the Universal Power be within you and your loved ones, now and always. Dave ------------------------------ Message: 10 Date: Tue, 29 Jul 2014 13:26:14 +0200 From: Christina Kreutzer Subject: [Histonet] Quartzy To: "" Message-ID: Content-Type: text/plain; charset=UTF-8 Hello everybody, we are currently thinking about a system to organize our lab-inventory and orders. Somebody recommended Quartzy to us (https://www.google.at/#q=quartzy+). Now I was wondering if anybody of you is using this system already, and what experiences you were having with it so far. Please share your thaughts. All the best Christina ------------------------------ Message: 11 Date: Tue, 29 Jul 2014 05:08:12 -0700 From: David Kemler Subject: [Histonet] Peggy Wenk To: Fellow HistoNetters Message-ID: <1406635692.31100.YahooMailBasic@web141106.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Peggy's last interview on HistoTalk www.histotalk.com?was taped last year at the NSH. During her interview, I stopped taping several times, because the tears and my choking up were too much for me. Each time I stopped, Peggy smiled at me and said 'It's Ok, Dave, it's OK". It's a shame HistoTalk never got the recognition it truly deserved. Interviews like Peggy's have meaning for everyone in the profession. Over the years, HistoTalk has?had a small group of champions - Peggy was one of them! God bless. To "Mr. Peggy Wenk", May the Universal Power be within you and your loved ones, now and always. Dave ? ------------------------------ Message: 12 Date: Tue, 29 Jul 2014 16:24:01 +0300 From: "j.rowaihi" Subject: ??: [Histonet] Quartzy To: Christina Kreutzer , "" Message-ID: Content-Type: text/plain; charset=utf-8 I have no experience on it, But it looks very helpful system.?? Regards Jamal Rowaihi Sent from my cell phone -------- ?????????????? ?????????????? -------- ????: Christina Kreutzer ??????????????: ????????/????/???? ??:???? ?? (GMT+03:00) ??????: "" ??????????????: [Histonet] Quartzy Hello everybody, we are currently thinking about a system to organize our lab-inventory and orders. Somebody recommended Quartzy to us (https://www.google.at/#q=quartzy+). Now I was wondering if anybody of you is using this system already, and what experiences you were having with it so far. Please share your thaughts. All the best Christina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 29 Jul 2014 06:38:24 -0700 From: David Kemler Subject: [Histonet] Peggy Wenk Interview Link To: Histonet Message-ID: <1406641104.99062.YahooMailNeo@web141105.mail.bf1.yahoo.com> Content-Type: text/plain; charset=us-ascii The direct link is www.HistoTalk.net Yours, Dave ------------------------------ Message: 14 Date: Tue, 29 Jul 2014 09:53:14 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] Peggy Wenk To: David Kemler , Histonet Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF9D33C24@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="iso-8859-1" Thanks Dave. Yes she was so brave. Also HistoTalk is a great venue for histotechs all over the world to get to hear great educators and histology professional champions interviewed by you. Younger techs will see their names many times but may never be able to relate to or get to know their good examples set for our profession. But thanks to Histotalk they can go back and listen to what these former ones have accomplished and how they did it. Such an inspiration. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Kemler Sent: Monday, July 28, 2014 9:35 PM To: Histonet Subject: [Histonet] Peggy Wenk Peggy's last interview on HistoTalk www.histotalk.com?was taped last year at the NSH. During her interview, I stopped taping several times, because the tears and my choking up were too much for me. Each time I stopped, Peggy smiled at me and said 'It's Ok, Dave, it's OK". It's a shame HistoTalk never got the recognition it truly deserved. Interviews like Peggy's have meaning for everyone in the profession. Over the years, HistoTalk has?had a small group of champions - Peggy was one of them! God bless. To "Mr. Peggy Wenk", May the Universal Power be within you and your loved ones, now and always. Dave _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 29 Jul 2014 14:19:31 +0000 From: Bernice Frederick Subject: RE: [Histonet] Peggy Wenk To: David Kemler , Fellow HistoNetters Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F1C55@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="iso-8859-1" This is the announcement on the NSH page: http://nsh.org/content/peggy-wenk-true-mentor She will be missed! Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Kemler Sent: Tuesday, July 29, 2014 7:08 AM To: Fellow HistoNetters Subject: [Histonet] Peggy Wenk Peggy's last interview on HistoTalk www.histotalk.com?was taped last year at the NSH. During her interview, I stopped taping several times, because the tears and my choking up were too much for me. Each time I stopped, Peggy smiled at me and said 'It's Ok, Dave, it's OK". It's a shame HistoTalk never got the recognition it truly deserved. Interviews like Peggy's have meaning for everyone in the profession. Over the years, HistoTalk has?had a small group of champions - Peggy was one of them! God bless. To "Mr. Peggy Wenk", May the Universal Power be within you and your loved ones, now and always. Dave ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 29 Jul 2014 10:20:09 -0400 From: Subject: Re: [Histonet] Peggy Wenk To: David Kemler , Fellow HistoNetters Message-ID: <20140729142009.8074.qmail@server276.com> Content-Type: text/plain; charset="utf-8" Thank you Dave for your selfless contribution of preserving the legends of Histotechnology. The Georgia Society for Histotechnology supports you and we applaud you for this wonderful gift. Thank you for sharing this precious soul~ Heartfelt love and condolences Wanda K Simons GSH President > -------Original Message------- > From: David Kemler > To: Fellow HistoNetters > Subject: [Histonet] Peggy Wenk > Sent: Jul 29 '14 08:08 > > > Peggy's last interview > on HistoTalk www.histotalk.com??was taped last > year at the NSH. During her interview, I stopped taping > several times, because the tears and my choking up were too > much for me. Each time I stopped, Peggy smiled at me and > said 'It's Ok, Dave, it's OK". > It's > a shame HistoTalk never got the recognition it truly > deserved. Interviews like Peggy's have meaning for > ????everyone in the profession. Over the years, HistoTalk > has??had a > small group of champions - Peggy was one of them! God bless. > To "Mr. Peggy Wenk", May the Universal Power be > within you and your loved ones, now and > always. > Dave > ?? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 128, Issue 29 ***************************************** From JJennings <@t> thedermlab.com Wed Jul 30 17:58:16 2014 From: JJennings <@t> thedermlab.com (Jonathan Jennings) Date: Wed Jul 30 17:58:47 2014 Subject: [Histonet] Histology Tech Needed in Birmingham, Al Message-ID: <000f4250.0ce70be02a2e85e3@thedermlab.com> Full Time histology tech is needed for a dermatopath lab in Birmingham, Al. Relocation bonus available if applicable. Flexible hours. Great Benifits! On the job training offered and encouraged! If interested please email me at jjennings@thedermlab.com Jonathan Jennings DermLab Pathology Manager This email was sent from my mobile device. Please excuse any typing errors From hans <@t> histologistics.com Wed Jul 30 19:14:13 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Wed Jul 30 19:14:19 2014 Subject: [Histonet] Schiff : room temp or not In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011251D957@JERRY.Gia.com> References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> <5A33C952BB67F4468AF1F36D739212BC011251D957@JERRY.Gia.com> Message-ID: Hello Amber, That depends on your protocol. Which protocol are you using and what does your protocol say to use the schiffs at? Consider that schiffs is usually stored at 4C in brown or opaque bottles. This suggests it is light sensitive. Our protocol say to use the schiffs at 37C for 15 minutes but I have used it at 22C for 30 minutes and at 60C for 10 minutes with consistent results. We have found that using it at 60C reduces it's shelf life considerably and do not suggest it unless you are familiar with doing so. Good luck Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie wrote: > > When staining PAS's, does the Schiff need to be room temp or does it matter? Just curious about when to add it to our Sakura stainer. Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mikeykkk_3 <@t> live.com Wed Jul 30 19:32:18 2014 From: mikeykkk_3 <@t> live.com (Michael Backhus) Date: Wed Jul 30 19:32:24 2014 Subject: [Histonet] Schiff : room temp or not In-Reply-To: References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> <5A33C952BB67F4468AF1F36D739212BC011251D957@JERRY.Gia.com> Message-ID: Joint Commission told us we could not keep it in the refrigerator because the bottle said to store it at room temperature. We called the manufacturer and they faxed us a paper stating we could keep it in the fridge. However It wasn't easy to get the paper because the manufacturer prefers the shelf live to be shorter so we buy more. Mike Sent from my iPhone > On Jul 30, 2014, at 8:14 PM, Hans B Snyder wrote: > > Hello Amber, > > That depends on your protocol. Which protocol are you using and what > does your protocol say to use the schiffs at? Consider that schiffs > is usually stored at 4C in brown or opaque bottles. This suggests it > is light sensitive. Our protocol say to use the schiffs at 37C for 15 > minutes but I have used it at 22C for 30 minutes and at 60C for 10 > minutes with consistent results. We have found that using it at 60C > reduces it's shelf life considerably and do not suggest it unless you > are familiar with doing so. > > Good luck > Hans B Snyder > Histologistics > 60 Prescott Street > Worcester, MA 01605 > 508-308-7800 > hans@histologistics.com > > > On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie > wrote: >> >> When staining PAS's, does the Schiff need to be room temp or does it matter? Just curious about when to add it to our Sakura stainer. Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From asanjeet <@t> yahoo.com Wed Jul 30 20:44:15 2014 From: asanjeet <@t> yahoo.com (Sanjeet) Date: Wed Jul 30 20:44:24 2014 Subject: [Histonet] Fwd: SOP on embedding tissues of different sizes in same mold References: Message-ID: Sent from my iPhone Begin forwarded message: > From: Sanjeet > Date: July 30, 2014 at 9:15:25 PM EDT > To: "histonet-request@lists.utsouthwestern.edu" > Subject: SOP on embedding tissues of different sizes in same mold > > Hi > Does anyone in the Histo world have an SOP on how to embed tissues of different sizes in the same mold. Do you embed in different levels depending upon the size, the larger embedded first and the smallest at the end. Or do you embed all in the same plane regardless of the tissue size. Are you able to justify and salvage all tissue on the slide. > Thank you all in advance > Sanjeet > > > Sent from my iPhone From asanjeet <@t> yahoo.com Wed Jul 30 20:45:45 2014 From: asanjeet <@t> yahoo.com (Sanjeet) Date: Wed Jul 30 20:45:46 2014 Subject: [Histonet] Fwd: Embedding skin References: Message-ID: Sent from my iPhone Begin forwarded message: > From: Sanjeet > Date: July 30, 2014 at 9:27:46 PM EDT > To: "histonet-request@lists.utsouthwestern.edu" > Subject: Embedding skin > > Hi Histo techs > > Need some info on the correct orientation of skin tissue. Punch/ ellipse. Does anyone have a literature on this topic. I am used to embed large skin in an angle, the skin being on top. > Currently the place where I work have different protocol, the skin is embedded straight, the epidermis being right angled to the mold. > I find the section difficult to cut when the skin is embedded straight , the section are compressed and more chances scoring along the section. > > Thanks > Sanjeet > > Sent from my iPhone From hans <@t> histologistics.com Wed Jul 30 22:07:20 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Wed Jul 30 22:07:30 2014 Subject: [Histonet] Schiff : room temp or not In-Reply-To: References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> <5A33C952BB67F4468AF1F36D739212BC011251D957@JERRY.Gia.com> Message-ID: There is no doubt, manufactures prefer shorter life. This case is exempt when one is producing solutions for themselves. Histologistics Hans B Snyder 508.308.7800 hans@histologistics.com > On Jul 30, 2014, at 8:32 PM, Michael Backhus wrote: > > > Joint Commission told us we could not keep it in the refrigerator because the bottle said to store it at room temperature. We called the manufacturer and they faxed us a paper stating we could keep it in the fridge. However It wasn't easy to get the paper because the manufacturer prefers the shelf live to be shorter so we buy more. > Mike > Sent from my iPhone > >> On Jul 30, 2014, at 8:14 PM, Hans B Snyder wrote: >> >> Hello Amber, >> >> That depends on your protocol. Which protocol are you using and what >> does your protocol say to use the schiffs at? Consider that schiffs >> is usually stored at 4C in brown or opaque bottles. This suggests it >> is light sensitive. Our protocol say to use the schiffs at 37C for 15 >> minutes but I have used it at 22C for 30 minutes and at 60C for 10 >> minutes with consistent results. We have found that using it at 60C >> reduces it's shelf life considerably and do not suggest it unless you >> are familiar with doing so. >> >> Good luck >> Hans B Snyder >> Histologistics >> 60 Prescott Street >> Worcester, MA 01605 >> 508-308-7800 >> hans@histologistics.com >> >> >> On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie >> wrote: >>> >>> When staining PAS's, does the Schiff need to be room temp or does it matter? Just curious about when to add it to our Sakura stainer. Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> From gu.lang <@t> gmx.at Thu Jul 31 05:12:56 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jul 31 05:18:24 2014 Subject: AW: [Histonet] Fwd: SOP on embedding tissues of different sizes in same mold In-Reply-To: References: Message-ID: <000601cfaca8$000737f0$0015a7d0$@gmx.at> We avoid to put specimens of different size into one cassette in general (especially with biopsies). If I have too pieces of different size with a clear cut side, I put them both on the ground of the mold. I think one could never precisly put a specimen in a certain level into the mold, if it doesn't lie on the bottom. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sanjeet Gesendet: Donnerstag, 31. Juli 2014 03:44 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fwd: SOP on embedding tissues of different sizes in same mold Sent from my iPhone Begin forwarded message: > From: Sanjeet > Date: July 30, 2014 at 9:15:25 PM EDT > To: "histonet-request@lists.utsouthwestern.edu" > Subject: SOP on embedding tissues of different sizes in same mold > > Hi > Does anyone in the Histo world have an SOP on how to embed tissues of different sizes in the same mold. Do you embed in different levels depending upon the size, the larger embedded first and the smallest at the end. Or do you embed all in the same plane regardless of the tissue size. Are you able to justify and salvage all tissue on the slide. > Thank you all in advance > Sanjeet > > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Jul 31 05:24:59 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jul 31 05:25:13 2014 Subject: AW: [Histonet] Fwd: Embedding skin In-Reply-To: References: Message-ID: <000a01cfaca9$af3a7c90$0daf75b0$@gmx.at> As a first information, we use sliding microtomes with the knife set in an angel (called declination). We embed straight, because areas of same concistancy should be positioned in one line and in the cutting direction. Areas of more rigidity compress the smoother ones, if they are shifted in an angle. If you have problems with the orientation of the tissue in the block, just turn the blockholder. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sanjeet Gesendet: Donnerstag, 31. Juli 2014 03:46 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Fwd: Embedding skin Sent from my iPhone Begin forwarded message: > From: Sanjeet > Date: July 30, 2014 at 9:27:46 PM EDT > To: "histonet-request@lists.utsouthwestern.edu" > Subject: Embedding skin > > Hi Histo techs > > Need some info on the correct orientation of skin tissue. Punch/ ellipse. Does anyone have a literature on this topic. I am used to embed large skin in an angle, the skin being on top. > Currently the place where I work have different protocol, the skin is embedded straight, the epidermis being right angled to the mold. > I find the section difficult to cut when the skin is embedded straight , the section are compressed and more chances scoring along the section. > > Thanks > Sanjeet > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jul 31 09:36:46 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 31 09:36:53 2014 Subject: [Histonet] Schiff : room temp or not In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011251D957@JERRY.Gia.com> References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> <5A33C952BB67F4468AF1F36D739212BC011251D957@JERRY.Gia.com> Message-ID: <1406817406.51959.YahooMailNeo@web120402.mail.ne1.yahoo.com> All chemical reactions (= all histochemistry procedures) are governed by the Van't Hoff law that states that every 10?C the speed of the reaction doubles (if it is +10?C) or is reduced in half (if it is -10?C) so, if you use the Schiff reagent (that has to be stored at 6-8 ?C) directly, the reaction will take longer than if the reagent is let to "warm up" to room temperature. You can use it as you wish but if used cool the reaction will take longer and you will have to give it more time because otherwise the staining will be weaker. Ren? J.? On Wednesday, July 30, 2014 4:14 PM, Amber McKenzie wrote: When staining PAS's, does the Schiff need to be room temp or does it matter?? Just curious about when to add it to our Sakura stainer.? Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jul 31 09:41:53 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 31 09:41:59 2014 Subject: [Histonet] Schiff : room temp or not In-Reply-To: References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> <5A33C952BB67F4468AF1F36D739212BC011251D957@JERRY.Gia.com> Message-ID: <1406817713.21505.YahooMailNeo@web120403.mail.ne1.yahoo.com> ?This is another example of the ignorance level of some Joint Commission "experts" that really do not know what they are talking about. The Schiff reagent (the "hay yellow" solution of Basic Fucsin) is temperature sensitive and HAS to be stored refrigerated or it will lose its reactivity in just a few days. Ren? J. On Wednesday, July 30, 2014 8:34 PM, Michael Backhus wrote: ? ? ? ? Joint Commission told us we could not keep it in the refrigerator because the bottle said to store it at room temperature. We called the manufacturer and they faxed us a paper stating we could keep it in the fridge. However It wasn't easy to get the paper because the manufacturer prefers the shelf live to be shorter so we buy more. Mike Sent from my iPhone > On Jul 30, 2014, at 8:14 PM, Hans B Snyder wrote: > > Hello Amber, > > That depends on your protocol. Which protocol are you using and what > does your protocol say to use the schiffs at?? Consider that schiffs > is usually stored at 4C in brown or opaque bottles.? This suggests it > is light sensitive.? Our protocol say to use the schiffs at 37C for 15 > minutes but I have used it at 22C for 30 minutes and at 60C for 10 > minutes with consistent results.? We have found that using it at 60C > reduces it's shelf life considerably and do not suggest it unless you > are familiar with doing so. > > Good luck > Hans B Snyder > Histologistics > 60 Prescott Street > Worcester, MA 01605 > 508-308-7800 > hans@histologistics.com > > > On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie > wrote: >> >> When staining PAS's, does the Schiff need to be room temp or does it matter?? Just curious about when to add it to our Sakura stainer.? Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Thu Jul 31 11:13:17 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Jul 31 11:13:22 2014 Subject: [Histonet] Schiff : room temp or not In-Reply-To: <1406817713.21505.YahooMailNeo@web120403.mail.ne1.yahoo.com> References: <98AE5DDFDD7D204E9CE6C7AE28C0B27B0F8F410F@MAILD.grove.ad.uconn.edu> <5A33C952BB67F4468AF1F36D739212BC011251D957@JERRY.Gia.com> <1406817713.21505.YahooMailNeo@web120403.mail.ne1.yahoo.com> Message-ID: Not ALL Schiff's NEED to be stored in the refrigerator. I use a Schiff's from Newcomer and it says to store at 15-30C (59-86F). It comes with a long shelf life and stains beautifully and can be reused a couple of times all at room temp. So why would I want to put this in the refrigerator? Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From mjones <@t> metropath.com Thu Jul 31 12:07:15 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Thu Jul 31 12:07:48 2014 Subject: [Histonet] Cassette Printer Message-ID: Has anyone out there used the Primera automated cassette printer? Pros? Cons? We are close to the General Data model, however, this Primera looks kinda cool. Thank you for input, Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com From j.rowaihi <@t> alborglaboratories.com Thu Jul 31 12:18:54 2014 From: j.rowaihi <@t> alborglaboratories.com (j.rowaihi) Date: Thu Jul 31 12:20:13 2014 Subject: =?UTF-8?Q?=D8=B1=D8=AF:_Re:_[Histonet]_Schiff_:_room_temp_or_not?= Message-ID: I am totally agree with you Rene? I also experienced the same problem with CAP regarding Schiff's reagent storing. Regards Jamal Rowaihi Laboratory supervisor Al Borg Medical Laboratories *Sent from my cell phoner* -------- ??????? ??????? -------- ??: Rene J Buesa ???????: ????/??/?? ?:?? ? (GMT+03:00) ???: Michael Backhus ,Hans B Snyder ,histonet@lists.utsouthwestern.edu ???????: Re: [Histonet] Schiff : room temp or not ?This is another example of the ignorance level of some Joint Commission "experts" that really do not know what they are talking about. The Schiff reagent (the "hay yellow" solution of Basic Fucsin) is temperature sensitive and HAS to be stored refrigerated or it will lose its reactivity in just a few days. Ren? J. On Wednesday, July 30, 2014 8:34 PM, Michael Backhus wrote: ? ? ? ? ?? Joint Commission told us we could not keep it in the refrigerator because the bottle said to store it at room temperature. We called the manufacturer and they faxed us a paper stating we could keep it in the fridge. However It wasn't easy to get the paper because the manufacturer prefers the shelf live to be shorter so we buy more. Mike Sent from my iPhone > On Jul 30, 2014, at 8:14 PM, Hans B Snyder wrote: > > Hello Amber, > > That depends on your protocol. Which protocol are you using and what > does your protocol say to use the schiffs at?? Consider that schiffs > is usually stored at 4C in brown or opaque bottles.? This suggests it > is light sensitive.? Our protocol say to use the schiffs at 37C for 15 > minutes but I have used it at 22C for 30 minutes and at 60C for 10 > minutes with consistent results.? We have found that using it at 60C > reduces it's shelf life considerably and do not suggest it unless you > are familiar with doing so. > > Good luck > Hans B Snyder > Histologistics > 60 Prescott Street > Worcester, MA 01605 > 508-308-7800 > hans@histologistics.com > > > On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie > wrote: >> >> When staining PAS's, does the Schiff need to be room temp or does it matter?? Just curious about when to add it to our Sakura stainer.? Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Thu Jul 31 12:40:20 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Jul 31 12:40:29 2014 Subject: [Histonet] RE: Cassette Printer In-Reply-To: References: Message-ID: Yes, we have one. Our first one was a lemon, but was replaced at no cost. Ever since then we had no problems whatsoever with it. We like it, because it has a small foot print and the printing is very clear, even with bar codes. The cons might be, that you have to unload the slides after a while (maybe after 15 - 20 slides). But this is not even a negative for us. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Thursday, July 31, 2014 10:07 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Printer Has anyone out there used the Primera automated cassette printer? Pros? Cons? We are close to the General Data model, however, this Primera looks kinda cool. Thank you for input, Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Jul 31 13:34:25 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu Jul 31 13:36:03 2014 Subject: [Histonet] Schiff : room temp or not In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B7811FDDBF21@EMBX-CHAM2.cdc.gov> We purchase ours from Newcomer and it is stable at room temp. I check it periodically and have never had it go weak before the expiration date. That is one of the reasons I began using it was it was always ready to use. Newcomer does note that it is sensitive to humidity and to keep tightly closed. We wrap lab tape around the lid to aid with that. Jeanine H. Sanders -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of j.rowaihi Sent: Thursday, July 31, 2014 1:19 PM To: Rene J Buesa; Michael Backhus; Hans B Snyder; histonet@lists.utsouthwestern.edu Subject: ??: Re: [Histonet] Schiff : room temp or not I am totally agree with you Rene? I also experienced the same problem with CAP regarding Schiff's reagent storing. Regards Jamal Rowaihi Laboratory supervisor Al Borg Medical Laboratories *Sent from my cell phoner* -------- ??????? ??????? -------- ??: Rene J Buesa ???????: ????/??/?? ?:?? ? (GMT+03:00) ???: Michael Backhus ,Hans B Snyder ,histonet@lists.utsouthwestern.edu ???????: Re: [Histonet] Schiff : room temp or not ?This is another example of the ignorance level of some Joint Commission "experts" that really do not know what they are talking about. The Schiff reagent (the "hay yellow" solution of Basic Fucsin) is temperature sensitive and HAS to be stored refrigerated or it will lose its reactivity in just a few days. Ren? J. On Wednesday, July 30, 2014 8:34 PM, Michael Backhus wrote: ? ? ? ? ?? Joint Commission told us we could not keep it in the refrigerator because the bottle said to store it at room temperature. We called the manufacturer and they faxed us a paper stating we could keep it in the fridge. However It wasn't easy to get the paper because the manufacturer prefers the shelf live to be shorter so we buy more. Mike Sent from my iPhone > On Jul 30, 2014, at 8:14 PM, Hans B Snyder wrote: > > Hello Amber, > > That depends on your protocol. Which protocol are you using and what > does your protocol say to use the schiffs at?? Consider that schiffs > is usually stored at 4C in brown or opaque bottles.? This suggests it > is light sensitive.? Our protocol say to use the schiffs at 37C for 15 > minutes but I have used it at 22C for 30 minutes and at 60C for 10 > minutes with consistent results.? We have found that using it at 60C > reduces it's shelf life considerably and do not suggest it unless you > are familiar with doing so. > > Good luck > Hans B Snyder > Histologistics > 60 Prescott Street > Worcester, MA 01605 > 508-308-7800 > hans@histologistics.com > > > On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie > wrote: >> >> When staining PAS's, does the Schiff need to be room temp or does it matter?? Just curious about when to add it to our Sakura stainer.? Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PREISZNE <@t> mail.etsu.edu Thu Jul 31 13:53:07 2014 From: PREISZNE <@t> mail.etsu.edu (Preiszner, Johanna) Date: Thu Jul 31 13:53:14 2014 Subject: [Histonet] Helicobacter control tissue request Message-ID: Hi, could anyone send us some Helicobacter pylori control tissue? The commercial slides are disappointing for us...they cost a lot and not every slide has the bacteria. We are a small lab and just started doing GI..I just need a small piece of tissue until we have a positive specimen to use for control. Thank you, Hanna Preiszner East Tennessee State University/Quillen College of Medicine From jaylundgren <@t> gmail.com Thu Jul 31 15:07:04 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Jul 31 15:07:11 2014 Subject: [Histonet] RE: Noise reduction In-Reply-To: References: Message-ID: I don't know if it's your PA not being able to concentrate, or your transcriptionists complaining about noise on the recording, but have you considered solving the problem on the other end? Noise canceling headphones and/or a throat microphone for your PA. A real throat mic, (not a video game accessory) works by picking up vibrations of the throat with transducers, so you don't get background noise. Voila! No noise in your PA's ears, or on transcriptionist's recordings. And your histotechs can still party! Sincerely, Jay A. Lundgren, M.S., HTL, (ASCP) On Wed, Jul 30, 2014 at 5:25 PM, Tony Reilly wrote: > Hi LeAnn > > At a previous laboratory we had a half wall with glass from bench height > to the ceiling and a glass door. The door could be open or closed and the > glass while blocking out noise did not make the area feel confined from the > rest of the lab. The other advantages were that the air conditioning to > that room was independent and not recycled to the rest of the lab reducing > formalin fumes and if there was a formalin spill in that area it was easily > contained. > > Regards > Tony > > > Tony Reilly B.App.Sc, M.Sc > Chief Scientist Anatomical Pathology > Princess Alexandra Laboratory | Pathology Queensland | Health Support > Queensland > Department of Health | Queensland Government > Building 15, Level 1, > 199 Ipswich Road > Woolloongabba Qld 4102 > Ph: 07 3176 2412 > Mob: 0402139411 > Fax: 07 3176 2930 > Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang > Sent: Thursday, 31 July 2014 4:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Noise reduction > > We are trying to figure out an inexpensive way to try to block off our > grossing area to help with noise reduction when our Pathologist Assistant > does her dictation. Has anyone done anything in their labs that they would > be willing to share with me? We don't want something permanent like > building a wall, but rather something that can be opened up for parts of > the day. We have thought about having a curtain (like a privacy curtain > found in doctors' offices), but we are not sure that would be enough of a > reduction. Anyone have other ideas? > > Thanks much! > LeAnn > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > LeAnn Lang > Associates in Pathology > Practice Administrator > Phone: 715-847-0075 (ext 50259) > llang@aipathology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ******************************************************************************** > This email, including any attachments sent with it, is confidential and > for the sole use of the intended recipient(s). This confidentiality is not > waived or lost, if you receive it and you are not the intended > recipient(s), or if it is transmitted/received in error. > Any unauthorised use, alteration, disclosure, distribution or review of > this email is strictly prohibited. The information contained in this > email, including any attachment sent with it, may be subject to a statutory > duty of confidentiality if it relates to health service matters. > If you are not the intended recipient(s), or if you have received this > email in error, you are asked to immediately notify the sender by telephone > collect on Australia +61 1800 198 175 or by return email. You should > also delete this email, and any copies, from your computer system network > and destroy any hard copies produced. > If not an intended recipient of this email, you must not copy, distribute > or take any action(s) that relies on it; any form of disclosure, > modification, distribution and/or publication of this email is also > prohibited. > Although Queensland Health takes all reasonable steps to ensure this email > does not contain malicious software, Queensland Health does not accept > responsibility for the consequences if any person's computer inadvertently > suffers any disruption to services, loss of information, harm or is > infected with a virus, other malicious computer programme or code that may > occur as a consequence of receiving this email. > Unless stated otherwise, this email represents only the views of the > sender and not the views of the Queensland Government. > > ********************************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From RHayden <@t> stormontvail.org Thu Jul 31 15:13:05 2014 From: RHayden <@t> stormontvail.org (Hayden, Rebecca) Date: Thu Jul 31 15:13:11 2014 Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 30 In-Reply-To: <20140730170303.8D4A820149@imsva01.stormontvail.org> References: <20140730170303.8D4A820149@imsva01.stormontvail.org> Message-ID: I have recently started to take over the control blocks for our lab department. I want to use/make a form that I can show how many blocks I have approved for each stain and which blocks are in use. I have looked a little online to see if there is a form out there that already exists, but can not seem to find one. I know that I can make it if needed, but seeing if anyone knows of one that is already made. Thanks. Rebecca Hayden, HTL (ASCP) Stormont-Vail Healthcare ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Wednesday, July 30, 2014 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 128, Issue 30 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Processor Alarms (Cristi Stephenson) 2. Thank you! (Cristi) ---------------------------------------------------------------------- Message: 1 Date: Tue, 29 Jul 2014 11:33:35 -0700 From: Cristi Stephenson Subject: [Histonet] Processor Alarms To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hello Histoland, We have an ASP300S processor that is not currently monitored by Leica. I was wondering if anyone had an alarm system suggestion such that, in the event it does malfunction, it will notify a designated person. We are a private practice so there is no one in the building after "normal" working hours. Thanks in advance for any recommendations! Cristi ------------------------------ Message: 2 Date: Tue, 29 Jul 2014 12:56:52 -0700 From: Cristi Subject: [Histonet] Thank you! To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=utf-8 Thank you all for the responses on alarm systems for the processors. I knew they were out there but couldn't come up with the right combination for Google to understand what I was looking for ???? Sent from my iPhone ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 128, Issue 30 ***************************************** NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a doctor accepting new patients. Call (785) 354-5225. ****************************************************************************************************************** The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. ****************************************************************************************************************** From mburns <@t> atlanticurologyclinics.com Thu Jul 31 15:16:48 2014 From: mburns <@t> atlanticurologyclinics.com (Melissa Burns) Date: Thu Jul 31 15:16:53 2014 Subject: [Histonet] Gyn requisition Message-ID: <9AD5CF7F-7291-4DF2-99D0-E8E119AAB197@atlanticurologyclinics.com> I was wondering if anyone is willing to share a copy of your gyn or gyn oncology based requisitions? Trying to create one and was hoping for some helping getting started. Email or fax to 8433998932 if you're willing to help. Thanks in advance! Melissa Sent from my iPhone From mjones <@t> metropath.com Thu Jul 31 15:26:05 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Thu Jul 31 15:26:17 2014 Subject: [Histonet] RE: Histonet Digest, Vol 128, Issue 30 Message-ID: Rebecca, We use slide tray boxes with large index cards. We have first control slide - to prove block works. Then pre-cut control slides then one stained last control slide - to make sure entity is still there. And the block that is in use is stored in box with the slides. Index card notes control block, who cut controls, how many, date etc. We keep our slide boxes alphabetized and stored on shelving for easy use. Slide box is pulled anytime the controls get to 5 slides or less - Anytime a block is tested we keep a record (in our procedure manual) as to whether it is appropriate for that stain or not - it is numbered. We take block log sheet into pathologist to review controls - log sheet is a table with date, tissue type, block #, stain employed, acceptable for use, pathologist or technician initials. Does that help? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 7/31/14, 2:13 PM, "Hayden, Rebecca" wrote: >I have recently started to take over the control blocks for our lab >department. I want to use/make a form that I can show how many blocks I >have approved for each stain and which blocks are in use. I have looked >a little online to see if there is a form out there that already exists, >but can not seem to find one. I know that I can make it if needed, but >seeing if anyone knows of one that is already made. Thanks. > > >Rebecca Hayden, HTL (ASCP) >Stormont-Vail Healthcare > >________________________________________ >From: histonet-bounces@lists.utsouthwestern.edu >[histonet-bounces@lists.utsouthwestern.edu] on behalf of >histonet-request@lists.utsouthwestern.edu >[histonet-request@lists.utsouthwestern.edu] >Sent: Wednesday, July 30, 2014 12:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 128, Issue 30 > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. Processor Alarms (Cristi Stephenson) > 2. Thank you! (Cristi) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Tue, 29 Jul 2014 11:33:35 -0700 >From: Cristi Stephenson >Subject: [Histonet] Processor Alarms >To: histonet@lists.utsouthwestern.edu >Message-ID: > > >Content-Type: text/plain; charset=UTF-8 > >Hello Histoland, > >We have an ASP300S processor that is not currently monitored by Leica. I >was wondering if anyone had an alarm system suggestion such that, in the >event it does malfunction, it will notify a designated person. We are a >private practice so there is no one in the building after "normal" working >hours. > >Thanks in advance for any recommendations! > >Cristi > > >------------------------------ > >Message: 2 >Date: Tue, 29 Jul 2014 12:56:52 -0700 >From: Cristi >Subject: [Histonet] Thank you! >To: histonet@lists.utsouthwestern.edu >Message-ID: >Content-Type: text/plain; charset=utf-8 > >Thank you all for the responses on alarm systems for the processors. I >knew they were out there but couldn't come up with the right combination >for Google to understand what I was looking for ???? > >Sent from my iPhone > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 128, Issue 30 >***************************************** > >NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a >doctor accepting new patients. Call (785) 354-5225. > >************************************************************************** >**************************************** > >The information transmitted in this e-mail and in any replies and >forwards are for the sole use of the above individual(s) or entities and >may contain proprietary, privileged and/or highly confidential >information. Any unauthorized dissemination, review, distribution or >copying of these communications is strictly prohibited. If this e-mail >has been transmitted to you in error, please notify and return the >original message to the sender immediately at the above listed address. >Thank you for your cooperation. > >************************************************************************** >**************************************** > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet