[Histonet] marking tiny specimens

Wanda.Smith <@t> HCAhealthcare.com Wanda.Smith <@t> HCAhealthcare.com
Thu Jan 30 15:07:58 CST 2014


We use a dropper of hematoxylin at the grossing table and it stays on the tissue even after processing.

WANDA G. SMITH, HTL(ASCP)HT 
Pathology Supervisor 
TRIDENT MEDICAL CENTER 
9330 Medical Plaza Drive 
Charleston, SC  29406 
843-847-4586 
843-847-4296 fax 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder
Sent: Thursday, January 30, 2014 3:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] marking tiny specimens

I am processing some extremely small specimens - pin tip size.  These are eggs with a protein covering.  I have tried using eosin to color the tissues before processing but the color came out before paraffin.  The coating on the eggs will not absorb the dye.  Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed.  Thanks in advance, Cheryl

Cheryl Crowder, BA, HTL(ASCP)

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