[Histonet] Silver in Nucleolus

[jerry sedgewick]

I'm working with a lab that stained cat gonads with Warthin Starry. 
We're looking for Bartonella bacteria.  In the first batch that was 
stained, some nucleii filled with the silver stain, something I've see 
in published images.  In the second batch it appears that nucleolus 
filled with silver.  I haven't seen that in google searches, etc.  I 
wondered if others have seen the nucleolus fill with silver (or is this 
bartonella?).  I'm also wondering how to prevent this sort of thing with 
proper bench procedures.

Thanks!

Jerry Sedgewick