[Histonet] IHC-start-up

[Will Chappell]

Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. 

I would steer clear of any platforms that do not let you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. 

William Chappell 

Sent from my iPhone

> On Jan 23, 2014, at 11:49 AM, Erin Sarricks <esarricks <@t> gmail.com> wrote:
> 
> Hi all-
> 
> Our histopathology lab is looking to
> set up an IHC component to service the request of our clients.  All work is on animal tissue.  We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year.  Some  stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs.  Does anyone have any other advice on other machines we should look into?  Any information or advice would be greatly appreciated. Thank you!
> 
> Regards,
> 
> Erin Sarricks, HT (ASCP)
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