[Histonet] FISH technique and NBF as fixative

[Nicole Cosenza]

I need to do FISH with the universal bacterial probe EUB338. I am not 
too familiar with FISH. Can we fix the sample with NBF or is 4% 
paraformaldehyde better? The samples are acellular dermal matrix.  A 
quick lit search shows people using 4% paraformaldehyde, but the 
histologist that will be embedding the tissue said that NBF is gentler 
on tissue than 4% PFA...