[Histonet] Frozen section protocol on rat tendon and/or muscle as well

[Peter Petro]

Dear all,


Happy New Year.


We are planning to work on rat tendon and frozen section of tendon will be
performed.  I'd like to ask for a better protocol to preserve, process and
section tendon as we found there is a lot of ice crystal (holes) on
sections of muscle that seriously affect our subsequent staining.  Any
better protocol to freeze muscle is also welcomed. We don't have much
experience in handling frozen tissues.


Best Regards,

Peter