From lpwenk <@t> sbcglobal.net Wed Jan 1 06:50:37 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Jan 1 06:50:41 2014 Subject: [Histonet] Histology program/school? In-Reply-To: References: Message-ID: If anyone wants to find out where the HT or HTL NAACLS-accredited programs are in the US, go to www.naacls.org on the left, click on "Find a Program" Then you can click on Program Type and click on either HT or HTL. If you want just one state, like Georgia, you can click on that. Or don't click on a state, and you get all the programs under HT or HTL. HT in Georgia: Darton State College 2400 Gillionville Rd Albany, GA 31707 Ms. Nancy Beamon M.S., MT(ASCP) (229) 317-6846 nancy.beamon@darton.edu No HTL in Georgia. I believe Darton College has a distance learning program. But I believe you need to be working in a histology lab (lab assistant or beginning tech) that is willing to let you have time to use the microtome and do stains, as you have to mail in completed slide sets to demonstrate proficiency in sectioning and staining, and someone has to supervise your work (to prove you are doing it, not someone else). So contact Nancy Beamon to get more information. And then start calling all the labs in Atlanta, to see if anyone is willing to hire you, or take you on as a student/trainee. It's probably a long shot, but you can try. If you are interested in histotechnology, it might help to join the Georgia Society for Histotechnology, and start networking with some of the officers. Here's the society's webpage: http://www.histosearch.com/gsh/default.html Good luck! Peggy Wenk, HTL(ASCP)SLS -----Original Message----- From: Michelle Lamphere Sent: Tuesday, December 31, 2013 8:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology program/school? Are there any histology programs or school in the Atlanta, GA area? If not, what would be a good place to start (in Atlanta) for somebody who wants to become a histotech? Michelle Lamphere, HT(ASCP) Lead Tech, Histology Department of Anatomic Pathology 1935 Medical District Dr. Dallas, TX 75235 214.456.2318 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abeharry798 <@t> gmail.com Wed Jan 1 18:43:08 2014 From: abeharry798 <@t> gmail.com (Andrea) Date: Wed Jan 1 18:43:18 2014 Subject: [Histonet] FW: In-Reply-To: References: <8E6784497F13AC44A38383BCB11826AD55A228A2@TDCEXCHXM007.ihs.org> <8E6784497F13AC44A38383BCB11826AD55A228D6@TDCEXCHXM007.ihs.org> Message-ID: We just went live with the Lablion barcoding and tracking system. We have it interfaced with Meditech. It is a great system, the techs and PA's love it. Very intuitive, easy to use and they will customize the system to whatever your needs are. Andrea On 2013-12-31, at 10:14 AM, Tom McNemar wrote: > I am very happy with ours. Our system is from General Data. Specimen container labels are printed at accessioning, that label is scanned at grossing and cassettes are printed. Those cassettes are then scanned at the microtome and slide labels for that block only are printed from a small printer located at the microtome. > > We were unable to interface with our LIS (Meditech) so as a workaround, we have to print a report that sends the information over to the General Data system. > > There were some issues early on but it has proven to be a good system. I will be expanding it to include Cytology later this year. > > Tom McNemar, HT(ASCP) > Histology Supervisor > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burrage, Shannon L. > Sent: Tuesday, December 31, 2013 9:31 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FW: > > > > ________________________________ > From: Burrage, Shannon L. > Sent: Tuesday, December 31, 2013 8:10 AM > To: ,utsouthwestern.edu > Subject: > > > Hi: > > I'm new to this website, and anxious to learn from all. At this point, i'm wondering if anyone could give me any information on the general use of bar coding in histology. Such as available vendors, and necessary equipment, mostly your personal experiences with this system. > > > Thanks, > > Shannon > > shannon.burrage@unitypoint.org > > > > This message and accompanying documents are covered by the > Electronic Communications Privacy Act, 18 U.S.C. ?? 2510-2521, > and contain information intended for the specified individual(s) only. > This information is confidential. If you are not the intended recipient > or an agent responsible for delivering it to the intended recipient, you > are hereby notified that you have received this document in error and > that any review, dissemination, copying, or the taking of any action > based on the contents of this information is strictly prohibited. If you > have received this communication in error, please notify us immediately > by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From walkure2009 <@t> gmail.com Wed Jan 1 21:16:51 2014 From: walkure2009 <@t> gmail.com (Peter Petro) Date: Wed Jan 1 21:16:56 2014 Subject: [Histonet] Frozen section protocol on rat tendon and/or muscle as well Message-ID: Dear all, Happy New Year. We are planning to work on rat tendon and frozen section of tendon will be performed. I'd like to ask for a better protocol to preserve, process and section tendon as we found there is a lot of ice crystal (holes) on sections of muscle that seriously affect our subsequent staining. Any better protocol to freeze muscle is also welcomed. We don't have much experience in handling frozen tissues. Best Regards, Peter From mbmphoto <@t> gmail.com Thu Jan 2 00:04:37 2014 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Thu Jan 2 00:04:51 2014 Subject: [Histonet] Embedding paraffin for brain tissue? In-Reply-To: <52C2F3A5.9020102@mclean.harvard.edu> References: <52C2F3A5.9020102@mclean.harvard.edu> Message-ID: <60B22E77-E7BE-4CAF-BD11-770DA81F3147@gmail.com> Hello, I too have been experiencing the same difficulties cutting brain samples, I've tried 3 different types of paraffins - all the the same rolling of sections, some lines & tearing. It's not my microtome, because I've had it checked & it went through a recent general maintenance. I've tried several angle changes, used different brands of low profile coated blades & carefully watch the temperature of the tissue blocks. I'm also feeling very frustrated for not being able to cut good brain sections. Any assistance anyone can provide will be greatly appreciated. Maria Mejia UCSF Department of Neurology San Francisco, CA On Dec 31, 2013, at 8:41 AM, Tim Wheelock wrote: > Hi Everyone: > > In November, there was a discussion concerning different types of embedding paraffin. > Do people find that a certain kind of wax is preferable for embedding brain tissue, or doesn't it matter? > > I also use Surgipath EM-400, but have decided to try another brand. > I have been using this paraffin for about 25 years. > It used to cut like butter, with beautiful ribbons and no lines. > I would put the blocks on ice, wait 2 hours, and then cut 7-8 cases (about 190 slides) per day easily. > Now I am struggling to cut 4-5 cases. > I am experiencing a lot of lines in my sections and some rolling and tearing of parts of the sections. > This is affecting my lab's productivity, the quality of my sections, and is a source of constant frustration. > > I am cutting with Surgipath high profile Teflon coated blades. > When I tried Thermo-Fisher HP-35 Ultra blades, at first they helped, but soon I experienced rolling of the sections. > I have tried changing the angle of the blade, to no avail. > So, I thought that I would try a change in the embedding paraffin. > > Thanks for any suggestions that you may have, > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu Jan 2 02:50:28 2014 From: ree3 <@t> leicester.ac.uk (Edwards, Richard) Date: Thu Jan 2 02:50:40 2014 Subject: [Histonet] GMAfix. Message-ID: Hi All, a Happy New Year to all, my question is, is there any point in including iodoacetamide and phenylmethyl sulphonylchloride in the acetone( used at -20C) used for fixing biopsies prior to GMA processing/embedding?, many thanks. Richard Edwards University of Leicester U.K. From ignac324 <@t> gmail.com Thu Jan 2 06:27:44 2014 From: ignac324 <@t> gmail.com (Ignacio Ruz Caracuel) Date: Thu Jan 2 06:27:48 2014 Subject: [Histonet] Frozen section protocol on rat tendon and/or muscle as well In-Reply-To: References: Message-ID: Dear Peter: Our muscle freeze protocol is very easy. We employed a small piece of cork of about 1x1cm, we pour a drop of OCT embedding medium and we placed the muscle on it. It?s important not to pour a big drop of OCT, cause it creates holes in the muscle as it infiltrates. Then we cooled isopentane in liquid nitrogen (-80 degrees) for about 10 minutes, we disolved it in case it aggregates and then we freeze the muscle for about 20 second. If you don?t pour too much OCT and you achieve a fast freezing with the isopentane at the correct temperature you won?t have those disturbing holes. Best regards, Ignacio Ruz-Caracuel Histology Intern Student Faculty of Medicine, C?rdoba, SPAIN http://www.uco.es/regmus/ 2014/1/2 Peter Petro > Dear all, > > > Happy New Year. > > > We are planning to work on rat tendon and frozen section of tendon will be > performed. I'd like to ask for a better protocol to preserve, process and > section tendon as we found there is a lot of ice crystal (holes) on > sections of muscle that seriously affect our subsequent staining. Any > better protocol to freeze muscle is also welcomed. We don't have much > experience in handling frozen tissues. > > > Best Regards, > > Peter > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lpwenk <@t> sbcglobal.net Thu Jan 2 07:24:46 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jan 2 07:24:49 2014 Subject: [Histonet] Frozen section protocol on rat tendon and/or muscle aswell In-Reply-To: References: Message-ID: <0DCE366A3E2B4903B4BC945503F5B4A4@HP2010> Is the -80 degrees really a correct temp? We freeze our muscles around -150 to -160 Degrees C. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Ignacio Ruz Caracuel Sent: Thursday, January 02, 2014 7:27 AM To: Peter Petro ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen section protocol on rat tendon and/or muscle aswell Dear Peter: Our muscle freeze protocol is very easy. We employed a small piece of cork of about 1x1cm, we pour a drop of OCT embedding medium and we placed the muscle on it. It?s important not to pour a big drop of OCT, cause it creates holes in the muscle as it infiltrates. Then we cooled isopentane in liquid nitrogen (-80 degrees) for about 10 minutes, we disolved it in case it aggregates and then we freeze the muscle for about 20 second. If you don?t pour too much OCT and you achieve a fast freezing with the isopentane at the correct temperature you won?t have those disturbing holes. Best regards, Ignacio Ruz-Caracuel Histology Intern Student Faculty of Medicine, C?rdoba, SPAIN http://www.uco.es/regmus/ 2014/1/2 Peter Petro > Dear all, > > > Happy New Year. > > > We are planning to work on rat tendon and frozen section of tendon will be > performed. I'd like to ask for a better protocol to preserve, process and > section tendon as we found there is a lot of ice crystal (holes) on > sections of muscle that seriously affect our subsequent staining. Any > better protocol to freeze muscle is also welcomed. We don't have much > experience in handling frozen tissues. > > > Best Regards, > > Peter > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From argonautro <@t> yahoo.com Thu Jan 2 07:50:22 2014 From: argonautro <@t> yahoo.com (Pirici Daniel) Date: Thu Jan 2 07:50:30 2014 Subject: [Histonet] Embedding paraffin for brain tissue? In-Reply-To: <60B22E77-E7BE-4CAF-BD11-770DA81F3147@gmail.com> References: <52C2F3A5.9020102@mclean.harvard.edu> <60B22E77-E7BE-4CAF-BD11-770DA81F3147@gmail.com> Message-ID: <1388670622.70317.YahooMailNeo@web163404.mail.gq1.yahoo.com> Hi there, ? 1. Have you changed anything in the processing and embedding processes themselves?(dehydration, clearing agents and times, etc)? Friable sections could also come from extended times in?the clearing agent... ? 2. There is also a section transfer system with a waterfall. We have a microtome which has both systems to collect the ribbons (classical and with the waterfall). There is a huge difference between them, it is much easier to cut on the water-based system. We can cut even 100 seriate sections without losing even 1 section!!! And there are also Peltier-based cooling block clamps that help keeping your paraffin bloc cold during the cutting!!! There are many companies offering these options... ? But since you are experiencing a sudden change in the quality of the ribbons with the same paraffin initially, I would seriously consider first some change in the embedding protocol... ? I hope this might help! ? A HAPPY NEW YEAR TO ALL HISTONETT-ERS!!!!! ________________________________________________________________________________________________ Pirici Nicolae Daniel, MD, PhD Department of Histology University of Medicine and Pharmacy Craiova Petru Rares Street 2, 200349 Craiova, Dolj Romania ________________________________ From: Maria Mejia To: Tim Wheelock Cc: "histonet@lists.utsouthwestern.edu" Sent: Thursday, January 2, 2014 8:04 AM Subject: Re: [Histonet] Embedding paraffin for brain tissue? Hello, I too have been experiencing the same difficulties cutting brain samples, I've tried 3 different types of paraffins - all the the same rolling of sections, some lines & tearing.? It's not my microtome, because I've had it checked & it went through a recent general maintenance. I've tried several angle changes, used different brands of low profile coated blades & carefully watch the temperature of the tissue blocks. I'm also feeling very frustrated for not being able to cut good brain sections.? Any assistance anyone can provide will be greatly appreciated. Maria Mejia UCSF Department of Neurology San Francisco, CA On Dec 31, 2013, at 8:41 AM, Tim Wheelock wrote: > Hi Everyone: > > In November, there was a discussion concerning different types of embedding paraffin. > Do people find that a certain kind of wax is preferable for embedding brain tissue, or doesn't it matter? > > I also use Surgipath EM-400, but have decided to try another brand. > I have been using this paraffin for about 25 years. > It used to cut like butter, with beautiful ribbons and no lines. > I would put the blocks on ice, wait 2 hours, and then cut 7-8 cases (about 190 slides) per day easily. > Now I am struggling to cut 4-5 cases. > I am experiencing a lot of lines in my sections and some rolling and tearing of parts of the sections. > This is affecting my lab's productivity, the quality of my sections, and is a source of constant frustration. > > I am cutting with Surgipath high profile Teflon coated blades. > When I tried Thermo-Fisher HP-35 Ultra blades, at first they helped, but soon I experienced rolling of the sections. > I have tried changing the angle of the blade, to no avail. > So, I thought that I would try a change in the embedding paraffin. > > Thanks for any suggestions that you may have, > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jan 2 10:44:48 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Jan 2 10:47:23 2014 Subject: [Histonet] cryostat Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E724@HPEMX3.HealthPartners.int> Does anyone have any current information on the cryostats with UV for decontamination? Specifically, is it accepted as a means of decontamination by CAP or JS or any other regulatory agencies? Basically, is it worth the extra dollars to go that route? Much thanks and Happy 2014!! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Joyce.Weems <@t> emoryhealthcare.org Thu Jan 2 11:01:09 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Jan 2 11:01:23 2014 Subject: [Histonet] RE: Histology program/school? In-Reply-To: References: Message-ID: Darton College in Albany has an online associates program. I would start there. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere Sent: Tuesday, December 31, 2013 8:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology program/school? Are there any histology programs or school in the Atlanta, GA area? If not, what would be a good place to start (in Atlanta) for somebody who wants to become a histotech? Michelle Lamphere, HT(ASCP) Lead Tech, Histology Department of Anatomic Pathology 1935 Medical District Dr. Dallas, TX 75235 214.456.2318 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From wbenton <@t> cua.md Thu Jan 2 11:06:01 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Jan 2 11:07:10 2014 Subject: [Histonet] RE: cryostat In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E724@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E724@HPEMX3.HealthPartners.int> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF1E9@CUAEXH1.GCU-MD.local> ANP.23410 Cryostat Decontamination Phase II There is a documented procedure for the routine decontamination of the cryostat at defined intervals, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. This should be done at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. REFERENCES 1) Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers From Occupationally Acquired Infections; Approved Guideline?Third Edition. CLSI document M29-A3 (ISBN 1-56238-567-4). Clinical and Laboratory Standards Institute, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2005. 2) http://www.well.ox.ac.uk/_asset/file/leica-disinifection-2.pdf 3) http://www.epa.gov/oppad001/list_b_tuberculocide.pdf Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L [Dorothy.L.Webb@HealthPartners.Com] Sent: Thursday, January 02, 2014 11:44 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] cryostat Does anyone have any current information on the cryostats with UV for decontamination? Specifically, is it accepted as a means of decontamination by CAP or JS or any other regulatory agencies? Basically, is it worth the extra dollars to go that route? Much thanks and Happy 2014!! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From abilger <@t> wellspan.org Thu Jan 2 11:16:15 2014 From: abilger <@t> wellspan.org (Bilger, Andrea) Date: Thu Jan 2 11:16:46 2014 Subject: [Histonet] AFB control Message-ID: <6D7752544B308D44A902C0BD0EC7BF5C14EA0EF3DE@EXCH02.wellspan.org> Happy New Year everyone! Does anybody have an extra AFB control block they would be willing to give up? We aren't having any luck looking in past cases and tried to get help from our Micro dept. to make our own without much luck. Andrea Bilger York Hospital York, Pa. 17405 (717)851-5040 ________________________________ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information.. ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ From leila.etemadi <@t> med.lu.se Thu Jan 2 11:16:00 2014 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Thu Jan 2 11:52:55 2014 Subject: [Histonet] Histology program/school? In-Reply-To: References: Message-ID: <3B0B0349-4ADD-426D-BC1B-37C20A387491@med.lu.se> Is any one knows any school/Lab in Europe? On 02 Jan 2014, at 18:01, Weems, Joyce K. wrote: > Darton College in Albany has an online associates program. I would start there. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle Lamphere > Sent: Tuesday, December 31, 2013 8:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology program/school? > > Are there any histology programs or school in the Atlanta, GA area? If not, what would be a good place to start (in Atlanta) for somebody who wants to become a histotech? > > > > Michelle Lamphere, HT(ASCP) > Lead Tech, Histology > Department of Anatomic Pathology > 1935 Medical District Dr. > Dallas, TX 75235 > 214.456.2318 > > Please consider the environment before printing this e-mail > > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. > > > Please consider the environment before printing this e-mail > > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Jan 2 12:02:52 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jan 2 12:02:55 2014 Subject: [Histonet] Histology program/school? In-Reply-To: <3B0B0349-4ADD-426D-BC1B-37C20A387491@med.lu.se> References: <3B0B0349-4ADD-426D-BC1B-37C20A387491@med.lu.se> Message-ID: <73CDBB3884064FE1A2F567D1D56F3E24@HP2010> For Schools in the British Isles: http://www.nhshistopathology.net/ Click on Schools. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Leila Etemadi Sent: Thursday, January 02, 2014 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histology program/school? Is any one knows any school/Lab in Europe? On 02 Jan 2014, at 18:01, Weems, Joyce K. wrote: > Darton College in Albany has an online associates program. I would start > there. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems@emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's > Hospital and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. If > you are not the intended recipient, please delete this message, and reply > to the sender regarding the error in a separate email. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle > Lamphere > Sent: Tuesday, December 31, 2013 8:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology program/school? > > Are there any histology programs or school in the Atlanta, GA area? If > not, what would be a good place to start (in Atlanta) for somebody who > wants to become a histotech? > > > > Michelle Lamphere, HT(ASCP) > Lead Tech, Histology > Department of Anatomic Pathology > 1935 Medical District Dr. > Dallas, TX 75235 > 214.456.2318 > > Please consider the environment before printing this e-mail > > This e-mail, facsimile, or letter and any files or attachments transmitted > with it contains information that is confidential and privileged. This > information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the > intended recipient, further disclosures are prohibited without proper > authorization. If you are not the intended recipient, any disclosure, > copying, printing, or use of this information is strictly prohibited and > possibly a violation of federal or state law and regulations. If you have > received this information in error, please notify Children's Medical > Center Dallas immediately at 214-456-4444 or via e-mail at > privacy@childrens.com. Children's Medical Center Dallas and its affiliates > hereby claim all applicable privileges related to this information. > > > Please consider the environment before printing this e-mail > > This e-mail, facsimile, or letter and any files or attachments transmitted > with it contains information that is confidential and privileged. This > information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the > intended recipient, further disclosures are prohibited without proper > authorization. If you are not the intended recipient, any disclosure, > copying, printing, or use of this information is strictly prohibited and > possibly a violation of federal or state law and regulations. If you have > received this information in error, please notify Children's Medical > Center Dallas immediately at 214-456-4444 or via e-mail at > privacy@childrens.com. Children's Medical Center Dallas and its affiliates > hereby claim all applicable privileges related to this information. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Elizabeth.Cameron <@t> jax.org Thu Jan 2 14:06:43 2014 From: Elizabeth.Cameron <@t> jax.org (Elizabeth Cameron) Date: Thu Jan 2 14:06:48 2014 Subject: [Histonet] Betagalactosidase/LacZ Message-ID: I have a question Lac Z staining and BetaGalactosidase IHC. The Lac-Z stain is enzyme dependent, so it must be fresh/frozen for the reaction to work. Is this also true for the betagalactosidase IHC? The target of the antibody is the enzyme, but I am not sure if the enzyme need to be "active" or just present for the antibody to bind to it. If anyone has knowledge on the subject, please share! We are working with mouse kidneys, and although our controls are positive, the kidneys that should be are not. Thank you, Liz The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From cmconway <@t> usgs.gov Thu Jan 2 15:02:01 2014 From: cmconway <@t> usgs.gov (Conway, Carla) Date: Thu Jan 2 15:02:24 2014 Subject: [Histonet] Fixed tissue storage ratio in 70% ethanol Message-ID: Hello, When transferring formalin-fixed tissues to 70% ethanol for storage, is there a recommended ratio of ethanol volume to tissue volume? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov From LSebree <@t> uwhealth.org Thu Jan 2 15:10:35 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jan 2 15:10:41 2014 Subject: [Histonet] Beta-Amyloid antibody, clone 6E10 availability Message-ID: <77DD817201982748BC67D7960F2F76AF08C6D5@UWHC-MBX12.uwhis.hosp.wisc.edu> So Histonet land, our usual vendor for this antibody can no longer get this clone. My question is: does anyone know who might hold the patent on this clone or at least know any company selling the 6E10 clone? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From brett_connolly <@t> merck.com Thu Jan 2 15:17:27 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Jan 2 15:17:31 2014 Subject: [Histonet] RE: Beta-Amyloid antibody, clone 6E10 availability In-Reply-To: <77DD817201982748BC67D7960F2F76AF08C6D5@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF08C6D5@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Linda, We get ours from Covance. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, January 02, 2014 4:11 PM To: Histonet (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] Beta-Amyloid antibody, clone 6E10 availability So Histonet land, our usual vendor for this antibody can no longer get this clone. My question is: does anyone know who might hold the patent on this clone or at least know any company selling the 6E10 clone? Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Fawaz.Zouabi <@t> sswahs.nsw.gov.au Thu Jan 2 15:54:27 2014 From: Fawaz.Zouabi <@t> sswahs.nsw.gov.au (Fawaz Zouabi) Date: Thu Jan 2 15:56:08 2014 Subject: [Histonet] Embedding paraffin for brain tissue? Message-ID: <04F39AE5B71E6D4CAAB4E1F2CB5393F818E146@sswlivma02.intra.swsahs.nsw.gov.au> Hi All Before looking at the wax issue, have you tried changing/looking at the processing issue first? We cut our human brain tissues (routine and large ) daily and we don't have any problem . We found that brain specs are better processed on a 5 days cycle, making them easier to handle ,cut and stain. Fawaz Zouabi Histo-Technologist Department of Forensic Medicine Glebe NSW Forensic & Analytical Science Service - FASS P O Box 90 Glebe NSW 2037 | Tel +612 8584 7842 | Fax +612 95664573 _____________________________________________________________________ This email has been scanned for the Sydney & South Western Sydney Local Health Districts by the MessageLabs Email Security System. Sydney & South Western Sydney Local Health Districts regularly monitor email and attachments to ensure compliance with the NSW Ministry of Health's Electronic Messaging Policy. From madeathridge <@t> pastnashville.com Thu Jan 2 16:34:02 2014 From: madeathridge <@t> pastnashville.com (Mary Ann Deathridge) Date: Thu Jan 2 16:34:53 2014 Subject: [Histonet] Hot Topic Medicare Code Message-ID: <1f9d9021$2a9e41a2$6632cc8c$@com> HAPPY NEW YEAR HISTONETTERS! Ok, so here is a question for 2014 coders. How do you code ( or should I say get to code) a doublestain for Medicare and for non-medicare? G0461 and G0462 (Medicare) and 88342 and 88343 for non-medicare. Can you charge for both antibodies in the doublestain or do you only charge one code and eat the second antibody on the same slide. Thanks for replies in advance. Maryann Deathridge, BS, HT (ASCP) Lab Manager Pathology Assoc.. of St. Thomas 615-298-4100, Fax: 615-298-4141 From wbenton <@t> cua.md Thu Jan 2 17:17:48 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Jan 2 17:18:44 2014 Subject: [Histonet] Hot Topic Medicare Code In-Reply-To: <1f9d9021$2a9e41a2$6632cc8c$@com> References: <1f9d9021$2a9e41a2$6632cc8c$@com> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF1F4@CUAEXH1.GCU-MD.local> AMA prescribes codes 88342 and 88343 for qualitative IHC (Non-Medicare): The AMA's CPT-2014 codebook offers two codes for reporting with qualitative immunohistochemistry (IHC) testing. The codes and official parenthetical instructions for their use starting Jan. 1 are as follows: 88342 Immunohistochemistry or immunocytochemistry, each separately identifiable antibody per block, cytologic preparation, or hematologic smear; first separately identifiable antibody per slide (Do not report 88342 in conjunction with 88360 or 88361 for the same antibody) (For quantitative or semi-quantitative immunohistochemistry, see 88360, 88361) 88343 each additional separately identifiable antibody per slide (List separately in addition to code for primary procedure) (Use 88343 in conjunction with 88342) (When multiple antibodies are applied to the same slide, use one unit of 88342 for the first separately identifiable antibody and one unit of 88343 for each additional identifiable antibody) CMS prescribes codes G0461 and G0462 for qualitative IHC: CMS will not accept CPT code 88342 or 88343 on a claim effective with dates of service on and after Jan. 1, 2014: Those two codes are "not valid for Medicare purposes" and will be summarily denied if billed. To report a professional, technical or global charge for qualitative immunohistochemistry (IHC) testing for a Medicare beneficiary on and after Jan. 1, 2014, you must use the applicable HCPCS Level II code as follows: G0461 Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain G0462 each additional single or multiplex antibody stain (List separately in addition to code for primary procedure) Codes G0461 and G0462 have 26 and TC modifier lines in the 2014 physician fee schedule, so you'll bill them using the modifier (or no modifier, if entitled to bill the global service) that applies to your practice and any given Medicare beneficiary claim. CMS prescribes that you continue to bill for qualitative IHC testing 'per specimen' as you have since Jan. 1, 2012. Furthermore, you'll continue to bill for quantitative IHC testing 'per specimen' using CPT codes 88360 and 88361 just as you do today and have done since Jan. 1, 2012. Effective January 1, 2014, per CMS (Medicare) all prostate needle biopsies, any method will be reported with the appropriate HCPCS code. They are: G0416-Surgical pathology, gross and microscopic examination for prostate needle biopsies, any method: 10 to 20 specimens (This is the code that pertains to most of our Prostate Needle Biopsy Cases) G0417- Surgical pathology, gross and microscopic examination for prostate needle biopsies, any method: 21 to 40 specimens G0418- Surgical pathology, gross and microscopic examination for prostate needle biopsies, any method: 41 to 60 specimens G0419- Surgical pathology, gross and microscopic examination for prostate needle biopsies, any method: greater than 60 specimens Per CMS, the descriptor was changed to say "any method" and is no longer tied to the surgical approach. So now it will be the count of individual biopsies that will tell you how to code your case. If you have 12 vials, you will report G0416. Please note that for nine or less you will continue to report 88305 per the number of separately identified biopsies. This will apply for CMS patients nationwide. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Ann Deathridge [madeathridge@pastnashville.com] Sent: Thursday, January 02, 2014 5:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hot Topic Medicare Code HAPPY NEW YEAR HISTONETTERS! Ok, so here is a question for 2014 coders. How do you code ( or should I say get to code) a doublestain for Medicare and for non-medicare? G0461 and G0462 (Medicare) and 88342 and 88343 for non-medicare. Can you charge for both antibodies in the doublestain or do you only charge one code and eat the second antibody on the same slide. Thanks for replies in advance. Maryann Deathridge, BS, HT (ASCP) Lab Manager Pathology Assoc.. of St. Thomas 615-298-4100, Fax: 615-298-4141 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Dorothy.L.Webb <@t> HealthPartners.Com Fri Jan 3 09:00:18 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Jan 3 09:02:48 2014 Subject: [Histonet] Congo red stain Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E734@HPEMX3.HealthPartners.int> We have been experiencing major difficulty with our Congo Red stain. We used to do it on the Artisan, but they changed raw products which has altered the stain and is not acceptable by our hematopathologists. Now we are back to manual and cannot seem to get it consistently acceptable. I would appreciate any ideas from the experts out there and if you want to send me offline your procedure, would be appreciated. Also, does anyone use a negative control , such as a keloid, on the slide? Thanks, as always, for your help! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Joyce.Weems <@t> emoryhealthcare.org Fri Jan 3 09:08:43 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jan 3 09:08:53 2014 Subject: [Histonet] Hot Topic Medicare Code In-Reply-To: <1f9d9021$2a9e41a2$6632cc8c$@com> References: <1f9d9021$2a9e41a2$6632cc8c$@com> Message-ID: And is the issues with double/triple stains resolved? Are the double codes for these only or for multiple blocks on the same specimen? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Ann Deathridge Sent: Thursday, January 02, 2014 5:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hot Topic Medicare Code HAPPY NEW YEAR HISTONETTERS! Ok, so here is a question for 2014 coders. How do you code ( or should I say get to code) a doublestain for Medicare and for non-medicare? G0461 and G0462 (Medicare) and 88342 and 88343 for non-medicare. Can you charge for both antibodies in the doublestain or do you only charge one code and eat the second antibody on the same slide. Thanks for replies in advance. Maryann Deathridge, BS, HT (ASCP) Lab Manager Pathology Assoc.. of St. Thomas 615-298-4100, Fax: 615-298-4141 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From abestul092456 <@t> gmail.com Fri Jan 3 09:12:08 2014 From: abestul092456 <@t> gmail.com (Andrea Bestul) Date: Fri Jan 3 09:12:13 2014 Subject: [Histonet] job resources Message-ID: Hello all, I am a new ASCP(HT) with a state (FL) HTL license, and am interested in positions in the greater Philadelphia area (including Wilmington, DE and south NJ). Any suggestions about good employers for starting out? Thank you so much. Andrea Bestul MLA II - Histology Baptist Medical Center Jacksonville, FL From KSimeone <@t> leavittmgt.com Fri Jan 3 10:22:38 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Fri Jan 3 10:22:47 2014 Subject: [Histonet] IHC QC Message-ID: Hello and happy new year! I was wondering what other labs (NOT CAP certified) are doing for their daily QC sign off on each antibody run? I have about 5 docs and each order anywhere from 2-50 IHC stains daily. Currently I use the Leica Bond and I am pulling and printing case logs (brief slide history) from each patient for the docs to sign off. It is A LOT of paperwork. Was hoping to reduce this and streamline it. The Bond's specific printing capabilities are pretty limited. Do you just have a daily QC list they sign? Would you mind sharing that with me? Any suggestions? I really appreciate the input! Thanks again. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From joelleweaver <@t> hotmail.com Fri Jan 3 10:58:45 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Jan 3 10:58:49 2014 Subject: [Histonet] IHC QC In-Reply-To: References: Message-ID: I am CAP. But yes I have a daily/per slide QC sign off technical AND pathologist that has scoring criteria so I get a quantitative score. I would suggest just developing that from your personal QA records, previous technical issues, repeated cases. I use the bond paperwork and tracking primarily during validation. It is not too tedious if you have history. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 3 Jan 2014 11:22:38 -0500 > From: KSimeone@leavittmgt.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC QC > > Hello and happy new year! I was wondering what other labs (NOT CAP certified) are doing for their daily QC sign off on each antibody run? I have about 5 docs and each order anywhere from 2-50 IHC stains daily. Currently I use the Leica Bond and I am pulling and printing case logs (brief slide history) from each patient for the docs to sign off. It is A LOT of paperwork. Was hoping to reduce this and streamline it. The Bond's specific printing capabilities are pretty limited. Do you just have a daily QC list they sign? Would you mind sharing that with me? Any suggestions? I really appreciate the input! > Thanks again. > > Kari M Simeone > Histology/Immunohistochemistry Specialist Supervisor > Alternate Laboratory Supervisor > ksimeone@leavittmgt.com > > > The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis <@t> seattlechildrens.org Fri Jan 3 11:03:19 2014 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri Jan 3 11:03:26 2014 Subject: [Histonet] Replacment parts for a Stovall Belly dancer bench top shaker. Message-ID: <3903BE18914F4440834F0E620415FFCA38208147@PPWEXD01a.childrens.sea.kids> Hi everyone, Does anyone know where I can get replacement parts for a Stovall Belly Dancer bench top shaker? One of the 4 tubing legs has snapped near the base. I might be able to jury-rig it with wire and duct tape, but it does have screws at each end of the tubing so It looks like the tubing legs should be replaceable. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From anolan <@t> prometheushealthcare.com Fri Jan 3 12:05:03 2014 From: anolan <@t> prometheushealthcare.com (anolan@prometheushealthcare.com) Date: Fri Jan 3 12:03:04 2014 Subject: [Histonet] Grossing Opportunities- West Chester County, NY Message-ID: <000601cf08ae$55602610$00207230$@prometheushealthcare.com> Hi All, I'm recruiting for a few new grossing opportunities in West Chester County, NY. Please contact me directly for further information. Anna Nolan - Recruiter Prometheus Healthcare Direct Line 301-693-8908 Office 301-693-9057 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com From turkekul <@t> gmail.com Fri Jan 3 12:58:55 2014 From: turkekul <@t> gmail.com (Mesru T) Date: Fri Jan 3 12:59:00 2014 Subject: [Histonet] beta gal antibody Message-ID: Hi Elizabeth, I always read and benefit from the messages here. Now I so happy that I think I might be of some help to you or also others. When I do enzymehistochemistry to detect the beta galactosidase enzimatic activity using x-gal as a substrate, I mostly use fresh frozen tissues and fix the frozen sections in 4% PFA + 0.2% gluteraldehyde in PBS for 5min. When I do IHC I prefer to use paraffin sections (the morphology is superior and I find it easier to cut paraffin than frozne). Frozen sections wll work for IHC too. The enzyme does not need to be active for antibody to bind during IHC satining. I have tried many antibodies until I have found the one that works for me on FFPE mouse tissues: Beta-galactosidase from eBioscience cat#14-6773 lot#E05196-1631 I use it at 1ug/ml concentration. I hope this helps. Regards, Mesruh Turkekul mskcc.org From rsrichmond <@t> gmail.com Fri Jan 3 13:06:26 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Fri Jan 3 13:06:29 2014 Subject: [Histonet] Re: Fixed tissue storage ratio in 70% ethanol Message-ID: Carla Conway at the Western Fisheries Research Center, in Seattle, Washington state asks: < gmail.com Fri Jan 3 13:51:29 2014 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Jan 3 13:51:34 2014 Subject: [Histonet] ROS-1 Message-ID: Hi All, Does anyone have a case of lung tumor positive for ROS1 by either IHC or FISH? We are working on validating this by FISH and haven't seen a positive case. We are going to order a positive cell line but we'll be running this on tissue, so if anyone could spare a few slides or a block that would very helpful. thank you! Mark Tarango From joelleweaver <@t> hotmail.com Fri Jan 3 13:57:00 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Jan 3 13:57:04 2014 Subject: [Histonet] ROS-1 In-Reply-To: References: Message-ID: Boy I wish, I am in the same boat and unable to validate my FISH . If I find any cases I will share some slides. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 3 Jan 2014 11:51:29 -0800 > From: marktarango@gmail.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] ROS-1 > > Hi All, > > Does anyone have a case of lung tumor positive for ROS1 by either IHC or > FISH? We are working on validating this by FISH and haven't seen a > positive case. We are going to order a positive cell line but we'll be > running this on tissue, so if anyone could spare a few slides or a block > that would very helpful. > > thank you! > > Mark Tarango > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Fri Jan 3 13:58:36 2014 From: turkekul <@t> gmail.com (Mesru T) Date: Fri Jan 3 13:58:39 2014 Subject: [Histonet] beta gal antibody In-Reply-To: References: Message-ID: Hi James, For IHC: I fix the tissue after grossing in 4% PFA ( 10% NBF should work fine as well), then proecess for paraffin and embed. I have never tried this marker with gluteraldehyde fixation. Usually gluteraldehyde is not suggested for IHC. Mesru On Fri, Jan 3, 2014 at 2:26 PM, James Watson wrote: > Do you still fix with the 4%pfa + 0.2% glutaraldehyde or can the tissues > be fixed in formalin for paraffin sections? > > James Watson HT ASCP > GNF Genomics Institute of the Novartis Research Foundation > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mesru T > Sent: Friday, January 03, 2014 10:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] beta gal antibody > > Hi Elizabeth, > > I always read and benefit from the messages here. Now I so happy that I > think I might be of some help to you or also others. > > When I do enzymehistochemistry to detect the beta galactosidase enzimatic > activity using x-gal as a substrate, I mostly use fresh frozen tissues and > fix the frozen sections in 4% PFA + 0.2% gluteraldehyde in PBS for 5min. > When I do IHC I prefer to use paraffin sections (the morphology is > superior and I find it easier to cut paraffin than frozne). > Frozen sections wll work for IHC too. The enzyme does not need to be > active for antibody to bind during IHC satining. > > I have tried many antibodies until I have found the one that works for me > on FFPE mouse tissues: Beta-galactosidase from eBioscience cat#14-6773 > lot#E05196-1631 I use it at 1ug/ml concentration. > > I hope this helps. > > Regards, > Mesruh Turkekul > mskcc.org > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From vgrover <@t> GENERAL-DATA.com Fri Jan 3 16:18:42 2014 From: vgrover <@t> GENERAL-DATA.com (Grover, Valantou) Date: Fri Jan 3 16:18:55 2014 Subject: [Histonet] RE: Congo red stain In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E734@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E734@HPEMX3.HealthPartners.int> Message-ID: Dear Dorothy, In commercial preparations of Congo Red are pretty consistent but not pure, a yellow monoazo dye may be present as this impurity. Although this may not affect staining solutions of Congo Red stain are not stable in salt or alkali solutions, so these do need to be prepared fresh. You could always choose to under differentiate and use polarized light to distinguish non specific background by using polarized light. Try to view this birefringence in sections greater than that of 5 microns, usually Congo Red sections are cut thicker at 10 microns or above, depending on the protocol. Alcoholic fixatives, Bouin's, etc. Other have chosen to use Sirius Red in the protocol also known as Direct Red 80. You can always contact me offline if you would like. vgrover@general-data.com Best regards, Valantou -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, January 03, 2014 10:00 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Congo red stain We have been experiencing major difficulty with our Congo Red stain. We used to do it on the Artisan, but they changed raw products which has altered the stain and is not acceptable by our hematopathologists. Now we are back to manual and cannot seem to get it consistently acceptable. I would appreciate any ideas from the experts out there and if you want to send me offline your procedure, would be appreciated. Also, does anyone use a negative control , such as a keloid, on the slide? Thanks, as always, for your help! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email may contain confidential General Data Company, Inc. information: any unauthorized or improper disclosure, copying, distribution or use of the contents of this email and attached document(s) is prohibited and may be a criminal offense. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you received this email in error, please reply immediately to the sender & delete this message and the attached document(s) without disclosure. From cmconway <@t> usgs.gov Fri Jan 3 16:27:52 2014 From: cmconway <@t> usgs.gov (Conway, Carla) Date: Fri Jan 3 16:28:08 2014 Subject: [Histonet] Re: Fixed tissue storage ratio in 70% ethanol In-Reply-To: References: Message-ID: Thank you, Bob! Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmconway@usgs.gov On Fri, Jan 3, 2014 at 11:06 AM, Bob Richmond wrote: > Carla Conway at the Western Fisheries Research Center, in Seattle, > Washington state asks: > > < there a recommended ratio of ethanol volume to tissue volume?<< > > Once the tissue is entirely fixed, it can be stored in the smallest volume > of 70% ethanol that will cover it. In my residency autopsy wet tissue > samples in 70% ethanol were stored in sealed plastic bags in a small volume > of it, and were intended to last for decades. > > Bob Richmond > Samurai Pathologist > Maryville, Tennessee > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Sat Jan 4 14:57:10 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jan 4 14:57:14 2014 Subject: [Histonet] Re: Congo red stain Message-ID: Dorothy Webb asks about amyloid staining with Congo red. Anatech about twelve years announced a product they call Amyloid Red. I've never read about anybody trying it, though. At that time I posted on Histonet that Anatech offers "a product I'd certainly want to look at if I were trying to stain amyloid. What they have named 'Amyloid Red' they describe as a ureylene (rather than benzidine) based dye, which according to their MSDS is Direct red 72 which is C.I. 29200 and CAS#10114-26-6. It is supposed to replace Congo red in amyloid staining. Amyloid Red colors amyloid, with the same birefringence (polarization) properties as Congo red. Obsolete as a textile dye for many years, benzidine-based Congo red is now listed by OSHA as a carcinogen, and could go out of manufacture. http://www.anatechltdusa.com/ http://my.net-link.net/~anatech/www/anatech/MSDSfolder/AmyloidRedMSDS.html Another question I've asked repeatedly is whether amyloid produced in experimental animals is ever offered as a control material. Has anyone seen this? Bob Richmond Samurai Pathologist Maryville TN From mtitford <@t> aol.com Sat Jan 4 16:11:44 2014 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Sat Jan 4 16:11:49 2014 Subject: [Histonet] Histotechnology schools in the United KIngdom Message-ID: <8D0D7B11691CA87-1288-19850@webmail-m237.sysops.aol.com> A few days ago Peggy Wenk kindly listed a web site for histotechnology schools in the United Kingdom, but it was a little mis-leading. The site was for all job vacancies in the United Kindom hospitals, all part of the British " National Heath Service". Most of the jobs listed were for physicians who wished to train in histopathology, not histotechnology. There were a few positions for what we in the USA call Medical Laboratory Technologists, that in the UK, includes histotechnology. The system in the United Kingdom is quite different to that in the United States. It may have changed recently since I left there, but In don't think so. In the UK there are no "Histotechnology schools" as such. If you wish to persue a career in histotechnology and work with human tissues, as in a hospital, you have to be State Registered. To be State Registered you have to attend their degree level national certificate program run by their Institute of Biomedical Science. It includes micro, blood banking, chemistry, hematology, etc, including histology. It is hard! During that time, you rotate through the different sections of the hospital laboratory. When you finish, and pass the test, then you can apply for State Registration. They have advanced fellowships in histotechnology. On our own Histonet from time to time, technologists from England have complained about having to work so hard and being understaffed. Maybe it is too hard for normal people!. (Histology technicians not working in a hospital or not requiring State Registration can train "on the job" and do not have to be State Registered. Years ago there was a program run by the Institute of Science Technology for non medical histology technicians that included Histology in its last two years of a five year part time program, but I don't know if it still exists) Maybe some one from the United KIngdom can bring us all up to date. Regards Michael Titford Pathology USA Mobile AL USA From richard.wild <@t> wanadoo.fr Sun Jan 5 01:10:30 2014 From: richard.wild <@t> wanadoo.fr (richard wild) Date: Sun Jan 5 01:46:04 2014 Subject: [Histonet] software : edit listing on autostainer dako Message-ID: <52C90566.1040902@wanadoo.fr> Hi all, When you use Autostainer+ from Dako (but any autostainer does the same) you edit a log file (one is xxxxxxxx*.pgm* the other is xxxxxxxx*.pg*) It countains the informations that you put directly in the machine software. The procedure is rather long even if you use shortcuts. It would be great to create such a log file directly with your informations created with other software out of autostan soft. Minimal informations are the number of the block, slide number and name of antibody. All that would be in a small text format listing created with other tools. The actual log file is made with these informations and many visible and invisible ascii codes and a lot of logic. I could discover many but many problems remain. Moreover my software to create a new log file cannot manage all these invisible ascii codes. So I couldnt realise yet an easy soft that could transform a small organised list of numbers and name of reagents in a readable log for Autostainer. _Did someone succeed in that task ? _ These is for personnal use, not for commercial purpoise. Best Richard Here under an exemple of beginning of a xxxxxxxx*.pgm *file (it is in german and the hidden caracters are... still hidden) 31 ?Doppel |200 !B mit Puffer sp?len end.Enzym- blockierung mit Puffer sp?len Prim?r- antik?rper mit Puffer sp?len markiertes Polymer mit Puffer sp?len mit Puffer sp?len *Umsch Chromogen mit Puffer sp?len mit Puffer sp?len *Umsch ;;Prim?r- antik?rper mit Puffer sp?len Sekund?r- reagenz mit Puffer sp?len Terti?r- reagenz mit Puffer sp?len *Umsch Chromogen- ansatz Chromogen mit Puffer sp?len *Umsch Zusatzreagenzien mit Puffer sp?len  14242/10 Dr. zzz B1Y*2Y1Y 2YChemMate, Citrat, DAKO, S2031 CM Citrat { < | RT 1Y 3YMelanosom (HMB45), DAKO, N1545 HMB45 { < |  RT 1Y*2Y1Y*1Y*6Y2Y1Y*1Y*6Y8Y1Y 2YREAL LSAB, 2 AK, DAKO, K5005 2AK-K5005 { < |  RT 1Y*2YStrept-AP, DAKO, K5005 Strept-AP { < |  RT 1Y*6Y5Y2YRED, DAKO, K5005 RED { < | RT 1Y*6Y2YREAL-Hematoxylin, DAKO, S2020 Hema/4 { < |  RT 1Y* B1Y*2Y1Y 2YChemMate, Citrat, DAKO, S2031 CM Citrat { < | RT 1Y 3YMelanosom (HMB45), DAKO, N1545 HMB45 { < |  RT 1Y*2Y1Y*1Y*6Y2Y1Y*1Y*6Y8Y1Y 2YREAL LSAB, 2 AK, DAKO, K5005 2AK-K5005 { < |  RT 1Y*2YStrept-AP, DAKO, K5005 Strept-AP { < |  RT 1Y*6Y5Y2YRED, DAKO, K5005 RED { < | RT 1Y*6Y2YREAL-Hematoxylin, DAKO, S2020 Hema/4 { < |  RT 1Y* B1Y*2Y1Y 2YChemMate, Citrat, DAKO, S2031 CM Citrat { < | RT 1Y 3YMART-1 (mk), Zymed, 113-0534 MART-1/50 { < | RT 1Y*2Y1Y*1Y*6Y2Y1Y*1Y*6Y8Y1Y 2YREAL LSAB, 2 AK, DAKO, K5005 2AK-K5005 { < |  RT 1Y*2YStrept-AP, DAKO, K5005 Strept-AP { < |  RT 1Y*6Y5Y2YRED, DAKO, K5005 RED From dea.leslie <@t> gmail.com Sun Jan 5 15:42:49 2014 From: dea.leslie <@t> gmail.com (Deanna Leslie) Date: Sun Jan 5 15:42:52 2014 Subject: [Histonet] Soaking artifact Message-ID: Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP From hans <@t> histologistics.com Sun Jan 5 17:16:39 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Sun Jan 5 17:16:44 2014 Subject: [Histonet] Soaking artifact In-Reply-To: References: Message-ID: Hello Deanna, This sounds like BS to me, maybe just a idiosyncrasy with their management. They are probably set in their ways and refuse to change or believe in another way to do it. It might be that one person once had artifact and it caused an uproar for the pathologists who in turn implemented this rule. I know, that if some kinds of tissue is soaked too long, the tissue can bubble up out of the block and be rendered unusable. If I were you, I would just go along with their way of cutting and don't try to change them. Change is only possible if someone is open to it. Best Regards Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Sun, Jan 5, 2014 at 4:42 PM, Deanna Leslie wrote: > Has anybody in histoland ever heard of this? I have been cutting tissue > for 25 yrs and until recently I had never heard of this! > I am under contract to a facility and the supervisor there does not want > anybody to soak their tissue or use ice! Your are supposed to use the cold > plate, because as I have stated soaking them causing an artifact. I have > not disputed this because it is not my place or in my job discription as a > traveler. I am not even sure what it is supposed to look like or what type > of problems it causes. > > Thanks for listening! > Deanna Leslie HT ASCP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Sun Jan 5 20:40:48 2014 From: b427297 <@t> aol.com (b427297@aol.com) Date: Sun Jan 5 20:40:54 2014 Subject: [Histonet] Soaking artifact In-Reply-To: References: Message-ID: <8D0D89FD7785DEB-17E4-20491@webmail-m276.sysops.aol.com> Your supervisor is wrong, and inexperienced. What artifact? Some tissues MUST be soaked on wet ice, spleen, liver, eye lens, anything bloody - You just can't get quality sections without soaking some tissues. You can tell her I said so. I'd like to know what artifact she is 'seeing'. Jackie O'Connor -----Original Message----- From: Deanna Leslie To: histonet Sent: Sun, Jan 5, 2014 3:44 pm Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Mon Jan 6 02:28:55 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Mon Jan 6 02:29:28 2014 Subject: [Histonet] Soaking artifact In-Reply-To: <8D0D89FD7785DEB-17E4-20491@webmail-m276.sysops.aol.com> References: <8D0D89FD7785DEB-17E4-20491@webmail-m276.sysops.aol.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FAF348879@FWDCWPMSGCMS09.hca.corpad.net> Incredible! Lee Luna (who wrote the book on histology) always said" rehydrate! rehydrate, rehydrate" Where are these so called supervisors coming from??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of b427297@aol.com Sent: Sunday, January 05, 2014 9:41 PM To: dea.leslie@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Soaking artifact Your supervisor is wrong, and inexperienced. What artifact? Some tissues MUST be soaked on wet ice, spleen, liver, eye lens, anything bloody - You just can't get quality sections without soaking some tissues. You can tell her I said so. I'd like to know what artifact she is 'seeing'. Jackie O'Connor -----Original Message----- From: Deanna Leslie To: histonet Sent: Sun, Jan 5, 2014 3:44 pm Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Jan 6 06:54:20 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Jan 6 06:54:24 2014 Subject: [Histonet] Soaking artifact In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FAF348879@FWDCWPMSGCMS09.hca.corpad.net> References: , <8D0D89FD7785DEB-17E4-20491@webmail-m276.sysops.aol.com>, <4BF03F5404EBDE409AF9232DA74B9DED2FAF348879@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: Ha! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Susan.Walzer@HCAHealthcare.com > To: b427297@aol.com; dea.leslie@gmail.com; histonet@lists.utsouthwestern.edu > Date: Mon, 6 Jan 2014 02:28:55 -0600 > Subject: RE: [Histonet] Soaking artifact > CC: > > Incredible! Lee Luna (who wrote the book on histology) always said" rehydrate! rehydrate, rehydrate" Where are these so called supervisors coming from??? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of b427297@aol.com > Sent: Sunday, January 05, 2014 9:41 PM > To: dea.leslie@gmail.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Soaking artifact > > Your supervisor is wrong, and inexperienced. What artifact? Some tissues MUST be soaked on wet ice, spleen, liver, eye lens, anything bloody - You just can't get quality sections without soaking some tissues. You can tell her I said so. I'd like to know what artifact she is 'seeing'. > Jackie O'Connor > > > > -----Original Message----- > From: Deanna Leslie > To: histonet > Sent: Sun, Jan 5, 2014 3:44 pm > Subject: [Histonet] Soaking artifact > > > Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! > I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. > > Thanks for listening! > Deanna Leslie HT ASCP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From philip_manfre <@t> merck.com Mon Jan 6 06:58:28 2014 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Mon Jan 6 06:58:34 2014 Subject: [Histonet] Soaking artifact In-Reply-To: References: Message-ID: <558A4571351D0C42BD923F403F4198C4BA6B02F9A8@USCTMXP51014.merck.com> I have been sectioning 28 years and, like everyone, completely disagree, at least with respect to animal tissue. You can "oversoak" some tissues like brain and liver, but you can also cut through the over soaked region just by cranking the wheel a little under the puffy stuff is gone. Also, the oversoaking occurs usually through neglect - leaving the blocks on wet ice for a long time. My two cents. Phil Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deanna Leslie Sent: Sunday, January 05, 2014 4:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lpwenk <@t> sbcglobal.net Mon Jan 6 07:07:22 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jan 6 07:07:35 2014 Subject: [Histonet] Soaking artifact In-Reply-To: References: Message-ID: <0EF212543CDE4CF48DAA605555EF4C86@HP2010> The only "soaking" artifacts that I can think of would be caused by: - soaking too long in water (minutes instead of a few seconds) - soaking under-processed tissue in water In both cases, the tissue is supposed to be "protected" by the wax, and if it is not (under-processed), or if the faced block is in water too long, the tissue can start re-absorbing water. The tissue then turns white and swells out of the block. So all that "swelled out" tissue is cut away and lost when the tissue is put back on the microtomy for sectioning the ribbon. If you soak for just a few seconds, such as a gauze with water being held against the block on the microtome, after it has been faced, then you will get a little bit of water absorbed into just a few layers of cells. Just enough to cut 2-4 sections. And you won't see that swelling artifact. For those of you saying - "but I have to face all the blocks, put them back on ice and/or water while I cut a bunch more blocks, and then go back and cut each block" - that is an artifact also. You have over-dehydrated your tissue during processing, and you are putting back the water that you should not have taken out. Processing is supposed to remove the unbound water (not attached to proteins), and some of the bound water (attached to proteins), and leave some of the bound water (attached to proteins) in the tissue. If you HAVE to soak EVERY block for more than a couple of seconds, then you are wasting time rehydrating and wasting time while microtoming. Cut down the time in the alcohols on the tissue processor, to leave a little bound water in the tissues. And you can NOT processing little biopsies on the same long processing cycle as the larger pieces of tissue (uterus, breast, etc.). Those little biopsies will be over-dehydrated. They HAVE to be run on a separate cycle of much shorter time intervals (10-20 minutes in each solution (once fixed), instead of 45-60 minutes in each solution). You should be able (on nearly every tissue block) to rough trim the tissue, and immediately start cutting ribbons. Possibly, you will need to put an ice cube on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the paraffin hardness to match the hardness of the tissue. That being said, some tissues are naturally brittle or crumbly, and always need some water put back in the tissue, such as spleen or bloody tissue, but again, some wet gauze on the faced block for a few seconds should be enough time to get 2-4 sections. And that's all the tissue we usually need from those blocks. If you need more for IHC, put the wet gauze back on the faced block, and cut a few more sections. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Deanna Leslie Sent: Sunday, January 05, 2014 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From philip_manfre <@t> merck.com Mon Jan 6 07:18:43 2014 From: philip_manfre <@t> merck.com (Manfre, Philip) Date: Mon Jan 6 07:18:48 2014 Subject: [Histonet] Soaking artifact In-Reply-To: <0EF212543CDE4CF48DAA605555EF4C86@HP2010> References: <0EF212543CDE4CF48DAA605555EF4C86@HP2010> Message-ID: <558A4571351D0C42BD923F403F4198C4BA6B02F9CA@USCTMXP51014.merck.com> With respect, this may work in a non-industrial setting, but in Pharma, with high through-put, it is generally not possible to have individualized processing programs for a lot of different tissues. You have to find one or two that work for the majority and compensate with some soaking. Experience will help you know which tissues can't get left on too long. Again, you can usually get past areas that look over-soaked just by cutting through them as you also mentioned. Just be careful, everyone, with small lesions so you don't ace through them. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, January 06, 2014 8:07 AM To: Deanna Leslie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Soaking artifact The only "soaking" artifacts that I can think of would be caused by: - soaking too long in water (minutes instead of a few seconds) - soaking under-processed tissue in water In both cases, the tissue is supposed to be "protected" by the wax, and if it is not (under-processed), or if the faced block is in water too long, the tissue can start re-absorbing water. The tissue then turns white and swells out of the block. So all that "swelled out" tissue is cut away and lost when the tissue is put back on the microtomy for sectioning the ribbon. If you soak for just a few seconds, such as a gauze with water being held against the block on the microtome, after it has been faced, then you will get a little bit of water absorbed into just a few layers of cells. Just enough to cut 2-4 sections. And you won't see that swelling artifact. For those of you saying - "but I have to face all the blocks, put them back on ice and/or water while I cut a bunch more blocks, and then go back and cut each block" - that is an artifact also. You have over-dehydrated your tissue during processing, and you are putting back the water that you should not have taken out. Processing is supposed to remove the unbound water (not attached to proteins), and some of the bound water (attached to proteins), and leave some of the bound water (attached to proteins) in the tissue. If you HAVE to soak EVERY block for more than a couple of seconds, then you are wasting time rehydrating and wasting time while microtoming. Cut down the time in the alcohols on the tissue processor, to leave a little bound water in the tissues. And you can NOT processing little biopsies on the same long processing cycle as the larger pieces of tissue (uterus, breast, etc.). Those little biopsies will be over-dehydrated. They HAVE to be run on a separate cycle of much shorter time intervals (10-20 minutes in each solution (once fixed), instead of 45-60 minutes in each solution). You should be able (on nearly every tissue block) to rough trim the tissue, and immediately start cutting ribbons. Possibly, you will need to put an ice cube on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the paraffin hardness to match the hardness of the tissue. That being said, some tissues are naturally brittle or crumbly, and always need some water put back in the tissue, such as spleen or bloody tissue, but again, some wet gauze on the faced block for a few seconds should be enough time to get 2-4 sections. And that's all the tissue we usually need from those blocks. If you need more for IHC, put the wet gauze back on the faced block, and cut a few more sections. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Deanna Leslie Sent: Sunday, January 05, 2014 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lpwenk <@t> sbcglobal.net Mon Jan 6 07:34:29 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jan 6 07:34:34 2014 Subject: [Histonet] Soaking artifact In-Reply-To: <0EF212543CDE4CF48DAA605555EF4C86@HP2010> References: <0EF212543CDE4CF48DAA605555EF4C86@HP2010> Message-ID: <0EE7064708484B839113D36C85BB84A5@HP2010> One slight amendment - this applies to human tissue. Animal tissue has far less bound and unbound water to start with, so no matter how it's processed, it always ends up "dry". Therefore, longer soaking in water is needed. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Lee & Peggy Wenk Sent: Monday, January 06, 2014 8:07 AM To: Deanna Leslie ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Soaking artifact The only "soaking" artifacts that I can think of would be caused by: - soaking too long in water (minutes instead of a few seconds) - soaking under-processed tissue in water In both cases, the tissue is supposed to be "protected" by the wax, and if it is not (under-processed), or if the faced block is in water too long, the tissue can start re-absorbing water. The tissue then turns white and swells out of the block. So all that "swelled out" tissue is cut away and lost when the tissue is put back on the microtomy for sectioning the ribbon. If you soak for just a few seconds, such as a gauze with water being held against the block on the microtome, after it has been faced, then you will get a little bit of water absorbed into just a few layers of cells. Just enough to cut 2-4 sections. And you won't see that swelling artifact. For those of you saying - "but I have to face all the blocks, put them back on ice and/or water while I cut a bunch more blocks, and then go back and cut each block" - that is an artifact also. You have over-dehydrated your tissue during processing, and you are putting back the water that you should not have taken out. Processing is supposed to remove the unbound water (not attached to proteins), and some of the bound water (attached to proteins), and leave some of the bound water (attached to proteins) in the tissue. If you HAVE to soak EVERY block for more than a couple of seconds, then you are wasting time rehydrating and wasting time while microtoming. Cut down the time in the alcohols on the tissue processor, to leave a little bound water in the tissues. And you can NOT processing little biopsies on the same long processing cycle as the larger pieces of tissue (uterus, breast, etc.). Those little biopsies will be over-dehydrated. They HAVE to be run on a separate cycle of much shorter time intervals (10-20 minutes in each solution (once fixed), instead of 45-60 minutes in each solution). You should be able (on nearly every tissue block) to rough trim the tissue, and immediately start cutting ribbons. Possibly, you will need to put an ice cube on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the paraffin hardness to match the hardness of the tissue. That being said, some tissues are naturally brittle or crumbly, and always need some water put back in the tissue, such as spleen or bloody tissue, but again, some wet gauze on the faced block for a few seconds should be enough time to get 2-4 sections. And that's all the tissue we usually need from those blocks. If you need more for IHC, put the wet gauze back on the faced block, and cut a few more sections. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Deanna Leslie Sent: Sunday, January 05, 2014 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Mon Jan 6 08:54:06 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Jan 6 08:54:13 2014 Subject: [Histonet] Soaking artifact In-Reply-To: <0EE7064708484B839113D36C85BB84A5@HP2010> References: <0EF212543CDE4CF48DAA605555EF4C86@HP2010> <0EE7064708484B839113D36C85BB84A5@HP2010> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32D317D3AB@Mail2Node2.ad.uams.edu> Thanks Peggy and Amen! When you have people taking human tissue and facing 25 or 30 blocks at a time and then leaving them face down on ice with water it causes the issues you stated. Soaking is something that was far more common in the past as the tissue fixed on processors with less reagent (Technicons Monos, Duos and Ultras) and did not always come out well processed. The fact that we would throw everything, no matter the size from tiny biopsies to huge hunks of tissue in was normal practice. We follow the practice the person is complaining about and only soaking for tissue with special requirements, such as bloody. Everyone hated till they tried and realized it works better. The pathologists are happier as the tissue is not water logged and stains better. We have come a long way in the last 48 years I have been doing Histology and we need look at some old practices and update. Lee did say rehydrate however; he also said be careful and do not allow the tissue to swell out of the block. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, January 06, 2014 7:34 AM To: Deanna Leslie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Soaking artifact One slight amendment - this applies to human tissue. Animal tissue has far less bound and unbound water to start with, so no matter how it's processed, it always ends up "dry". Therefore, longer soaking in water is needed. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Lee & Peggy Wenk Sent: Monday, January 06, 2014 8:07 AM To: Deanna Leslie ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Soaking artifact The only "soaking" artifacts that I can think of would be caused by: - soaking too long in water (minutes instead of a few seconds) - soaking under-processed tissue in water In both cases, the tissue is supposed to be "protected" by the wax, and if it is not (under-processed), or if the faced block is in water too long, the tissue can start re-absorbing water. The tissue then turns white and swells out of the block. So all that "swelled out" tissue is cut away and lost when the tissue is put back on the microtomy for sectioning the ribbon. If you soak for just a few seconds, such as a gauze with water being held against the block on the microtome, after it has been faced, then you will get a little bit of water absorbed into just a few layers of cells. Just enough to cut 2-4 sections. And you won't see that swelling artifact. For those of you saying - "but I have to face all the blocks, put them back on ice and/or water while I cut a bunch more blocks, and then go back and cut each block" - that is an artifact also. You have over-dehydrated your tissue during processing, and you are putting back the water that you should not have taken out. Processing is supposed to remove the unbound water (not attached to proteins), and some of the bound water (attached to proteins), and leave some of the bound water (attached to proteins) in the tissue. If you HAVE to soak EVERY block for more than a couple of seconds, then you are wasting time rehydrating and wasting time while microtoming. Cut down the time in the alcohols on the tissue processor, to leave a little bound water in the tissues. And you can NOT processing little biopsies on the same long processing cycle as the larger pieces of tissue (uterus, breast, etc.). Those little biopsies will be over-dehydrated. They HAVE to be run on a separate cycle of much shorter time intervals (10-20 minutes in each solution (once fixed), instead of 45-60 minutes in each solution). You should be able (on nearly every tissue block) to rough trim the tissue, and immediately start cutting ribbons. Possibly, you will need to put an ice cube on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the paraffin hardness to match the hardness of the tissue. That being said, some tissues are naturally brittle or crumbly, and always need some water put back in the tissue, such as spleen or bloody tissue, but again, some wet gauze on the faced block for a few seconds should be enough time to get 2-4 sections. And that's all the tissue we usually need from those blocks. If you need more for IHC, put the wet gauze back on the faced block, and cut a few more sections. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Deanna Leslie Sent: Sunday, January 05, 2014 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jan 6 09:50:30 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jan 6 09:59:11 2014 Subject: [Histonet] Amyloid Red Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E744@HPEMX3.HealthPartners.int> Does anyone out there use the Amyloid Red stain from Anatech in place of the Congo Red dye? Due to the pricing, would like some critiques before I order it!!! Thanks, Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From tpodawiltz <@t> lrgh.org Mon Jan 6 11:04:49 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Jan 6 11:04:55 2014 Subject: [Histonet] Soaking artifact In-Reply-To: <0EF212543CDE4CF48DAA605555EF4C86@HP2010> References: <0EF212543CDE4CF48DAA605555EF4C86@HP2010> Message-ID: <38667E7FB77ECD4E91BFAEB8D9863863250130566D@LRGHEXVS1.practice.lrgh.org> I agree Peggy. Just one question. What is a small histology lab to do when they only have one processor and cannot run separate cycles and do not have staffing to run short cycles throughout the day? Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, January 06, 2014 8:07 AM To: Deanna Leslie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Soaking artifact The only "soaking" artifacts that I can think of would be caused by: - soaking too long in water (minutes instead of a few seconds) - soaking under-processed tissue in water In both cases, the tissue is supposed to be "protected" by the wax, and if it is not (under-processed), or if the faced block is in water too long, the tissue can start re-absorbing water. The tissue then turns white and swells out of the block. So all that "swelled out" tissue is cut away and lost when the tissue is put back on the microtomy for sectioning the ribbon. If you soak for just a few seconds, such as a gauze with water being held against the block on the microtome, after it has been faced, then you will get a little bit of water absorbed into just a few layers of cells. Just enough to cut 2-4 sections. And you won't see that swelling artifact. For those of you saying - "but I have to face all the blocks, put them back on ice and/or water while I cut a bunch more blocks, and then go back and cut each block" - that is an artifact also. You have over-dehydrated your tissue during processing, and you are putting back the water that you should not have taken out. Processing is supposed to remove the unbound water (not attached to proteins), and some of the bound water (attached to proteins), and leave some of the bound water (attached to proteins) in the tissue. If you HAVE to soak EVERY block for more than a couple of seconds, then you are wasting time rehydrating and wasting time while microtoming. Cut down the time in the alcohols on the tissue processor, to leave a little bound water in the tissues. And you can NOT processing little biopsies on the same long processing cycle as the larger pieces of tissue (uterus, breast, etc.). Those little biopsies will be over-dehydrated. They HAVE to be run on a separate cycle of much shorter time intervals (10-20 minutes in each solution (once fixed), instead of 45-60 minutes in each solution). You should be able (on nearly every tissue block) to rough trim the tissue, and immediately start cutting ribbons. Possibly, you will need to put an ice cube on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the paraffin hardness to match the hardness of the tissue. That being said, some tissues are naturally brittle or crumbly, and always need some water put back in the tissue, such as spleen or bloody tissue, but again, some wet gauze on the faced block for a few seconds should be enough time to get 2-4 sections. And that's all the tissue we usually need from those blocks. If you need more for IHC, put the wet gauze back on the faced block, and cut a few more sections. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Deanna Leslie Sent: Sunday, January 05, 2014 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From amurvosh <@t> advancederm.net Mon Jan 6 11:28:44 2014 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Mon Jan 6 11:28:50 2014 Subject: [Histonet] Paperwork Message-ID: <4AD6A4E531E8C943A730559B6B81DF07CB92C4@dc.Advancederm.net> OK, I have another CAP Vs CLIA question. Can we toss paperwork after 2 years with Clia, like you do CAP specifically Temperature and maintenance charts. Thanks Anne From lpwenk <@t> sbcglobal.net Mon Jan 6 11:31:09 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Jan 6 11:31:16 2014 Subject: [Histonet] Soaking artifact In-Reply-To: <38667E7FB77ECD4E91BFAEB8D9863863250130566D@LRGHEXVS1.practice.lrgh.org> References: <0EF212543CDE4CF48DAA605555EF4C86@HP2010> <38667E7FB77ECD4E91BFAEB8D9863863250130566D@LRGHEXVS1.practice.lrgh.org> Message-ID: <1F339F315B6F457395E890440E86CFB3@HP2010> In most labs, the processor runs all night long. Someone comes in very early in the morning, empties the processor, starts the purge cycle, and then starts embedding a lot of blocks. The tissue processor then sits, doing nothing, from after the purge in the early morning, until sometime in the late afternoon, when all the tissues are loaded in for the overnight run. That means the tissue processor is doing nothing for up to 12 hours during the daytime. How about, besides the overnight run, we can set up 1 or 2 other shorter runs during the day, with the small biopsies. How about - process all the large tissues overnight, but keep the little biopsies that you grossed all afternoon in formalin until the morning. Empty out the large tissues, purge the process, embed the large tissues and start microtoming them. After the purge is done, put the small biopsies from the afternoon on the tissue processor, and process them for 1.5-2 hours (and if your processor is able to process half a load, do that to save on reagents). Then embed them and start microtoming them. Purge the processor again. In the mean time, all morning, collect the small biopsies again. After lunch, short process all the small biopsies from the morning. Embed them in the afternoon, purge the processor again, and load up the overnight load. If you don't have time to microtome the morning small biopsies (that you embedded in the afternoon), someone can microtome them in the morning the next day. Either with the large overnight load, or have someone else come in early, and while the other people are embedding the large tissue overnight load, they can be microtoming the small biopsies that were embedded in previous afternoon. Yes, all of this will mean changes: - staggered hours that people will be coming in - processing, embedding and microtoming continuously throughout the day - someone might have to microtome more than someone else, or might have to embed more than someone else. But if you rotate jobs around, over the months, everyone ends up doing the same amount of work overall. This is a type of continuous work flow, and does lead to faster turn around time and efficiency. When our lab changed to this system (we are an 1100 bed hospital, with lots of tissues from our ORs, from outside hospitals and clinics and doctors offices, so lots and lots of blocks), it took getting everyone involved - people accessioning, grossing, the histotechs, and the pathologists (they were not going to get their slides in numerical order). We have short cycles, the overnight long cycles, some rush cycles, and long cycles for breast and autopsy brain. We actually have more than 3 runs, but then we are working almost 24/7. During the time we were switching to continuous work flow, we had a few histotechs off on maternity and/or medical leaves. And we got a couple more clients, so the work load increased. But because of the continuous work flow, we were able to handle the additional work without having to hire anyone. Whereas before, we would process ALL the blocks overnight, and then would have lots of people embedding lots of blocks first thing in the very early morning, and then having to put in the in order, and no one could start microtoming until all the blocks were embedding and in order (so some days the microtoming techs were sitting around with nothing to do for a time). Then, everyone had piles of blocks to cut, for blocks 100-150 were going to be cut hours and hours after everyone started microtoming blocks 1-50. Then, there were racks and racks of slide piling up to be stained with H&E (and at that point, we were still labeling after staining). So there were all these spots where tissue was being held up, unnecessarily: - waiting to be processed - waiting to be embedding - waiting to be microtomed - waiting to be stained - waiting to be labeled - then the pathologists had stacks of slides, waiting to be diagnosed - waiting for the reports to be typed With continuous flow, there is always some work coming through, but not large piles causing long waits. It just takes rethinking how you can use the processor more efficiently, which will make the rest of the work more efficient. And increase productivity and make turn around time faster. Peggy A. Wenk, HTL(ASCP) -----Original Message----- From: Podawiltz, Thomas Sent: Monday, January 06, 2014 12:04 PM To: Lee & Peggy Wenk ; Deanna Leslie ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Soaking artifact I agree Peggy. Just one question. What is a small histology lab to do when they only have one processor and cannot run separate cycles and do not have staffing to run short cycles throughout the day? Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, January 06, 2014 8:07 AM To: Deanna Leslie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Soaking artifact The only "soaking" artifacts that I can think of would be caused by: - soaking too long in water (minutes instead of a few seconds) - soaking under-processed tissue in water In both cases, the tissue is supposed to be "protected" by the wax, and if it is not (under-processed), or if the faced block is in water too long, the tissue can start re-absorbing water. The tissue then turns white and swells out of the block. So all that "swelled out" tissue is cut away and lost when the tissue is put back on the microtomy for sectioning the ribbon. If you soak for just a few seconds, such as a gauze with water being held against the block on the microtome, after it has been faced, then you will get a little bit of water absorbed into just a few layers of cells. Just enough to cut 2-4 sections. And you won't see that swelling artifact. For those of you saying - "but I have to face all the blocks, put them back on ice and/or water while I cut a bunch more blocks, and then go back and cut each block" - that is an artifact also. You have over-dehydrated your tissue during processing, and you are putting back the water that you should not have taken out. Processing is supposed to remove the unbound water (not attached to proteins), and some of the bound water (attached to proteins), and leave some of the bound water (attached to proteins) in the tissue. If you HAVE to soak EVERY block for more than a couple of seconds, then you are wasting time rehydrating and wasting time while microtoming. Cut down the time in the alcohols on the tissue processor, to leave a little bound water in the tissues. And you can NOT processing little biopsies on the same long processing cycle as the larger pieces of tissue (uterus, breast, etc.). Those little biopsies will be over-dehydrated. They HAVE to be run on a separate cycle of much shorter time intervals (10-20 minutes in each solution (once fixed), instead of 45-60 minutes in each solution). You should be able (on nearly every tissue block) to rough trim the tissue, and immediately start cutting ribbons. Possibly, you will need to put an ice cube on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the paraffin hardness to match the hardness of the tissue. That being said, some tissues are naturally brittle or crumbly, and always need some water put back in the tissue, such as spleen or bloody tissue, but again, some wet gauze on the faced block for a few seconds should be enough time to get 2-4 sections. And that's all the tissue we usually need from those blocks. If you need more for IHC, put the wet gauze back on the faced block, and cut a few more sections. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Deanna Leslie Sent: Sunday, January 05, 2014 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From kryan <@t> nfderm.com Mon Jan 6 13:08:13 2014 From: kryan <@t> nfderm.com (Kaye Ryan) Date: Mon Jan 6 13:08:36 2014 Subject: [Histonet] RE: Paperwork In-Reply-To: <4AD6A4E531E8C943A730559B6B81DF07CB92C4@dc.Advancederm.net> References: <4AD6A4E531E8C943A730559B6B81DF07CB92C4@dc.Advancederm.net> Message-ID: <3F7E492EAB8FF74BA683B709924FC5C0E1EBA5@nfda-exch-01.NFDA.com> Hi Anne, CLIA Sec. 493.1105 list the retention requirements for records, slides, blocks and tissue: (3) Analytic systems records. Retain quality control and patient test records (including instrument printouts, if applicable) and all analytic systems activities specified in Sec. Sec. 493.1252 through 493.1289 for at least 2 years. Hope this helps, Kaye Ryan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne Murvosh Sent: Monday, January 06, 2014 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperwork OK, I have another CAP Vs CLIA question. Can we toss paperwork after 2 years with Clia, like you do CAP specifically Temperature and maintenance charts. Thanks Anne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jjohns3 <@t> cpallab.com Mon Jan 6 14:35:35 2014 From: jjohns3 <@t> cpallab.com (Johns, Jill) Date: Mon Jan 6 14:36:06 2014 Subject: [Histonet] NCCI interpretation for ISH coding Message-ID: <6D7752544B308D44A902C0BD0EC7BF5C14EA03275D@EXCH02.wellspan.org> The following was taken from the NCCI manual, Chapter 10, effective 1/1/14: 9. The unit of service for in situ hybridization reported as CPT codes 88365, 88367, or 88368 is each probe staining procedure per specimen. If a single probe staining procedure for one or more probes is performed on multiple blocks from a surgical specimen, multiple slides from a cytologic specimen, or multiple slides from a hematologic specimen, only one unit of service may be reported for each separate specimen. Physicians should not report more than one unit of service for CPT codes 88365, 88367, or 88368 per specimen for a probe staining procedure even if it contains multiple separately interpretable probes. I'm wondering how other labs are interpreting this, because I'm not sure how we continue to offer FISH testing and not go "in the hole" financially, if we can only bill for ONE probe on ONE specimen, regardless of the actual number of interpretable probes (Example: currently, for a HER2 FISH test, we bill for 2 units of service--1 for the HER2 probe and 1 for the CEP17 probe--both of which have to be enumerated by a professional and a ratio generated). Any insight from others would be greatly appreciated....Thanks! Jill A. Johns, MT(ASCP)SH, QCym, CCy Manager of Molecular Pathology Central PA Alliance Laboratory (CPAL) 1803 Mt. Rose Ave., Suite C3/C4 York, PA 17403 phone: (717) 851-4320 fax: (717) 851-1450 email: jjohns3@cpallab.com ________________________________ CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information.. ______________________________________________________________________ This e-mail has been scanned by Verizon Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on Verizon's Managed Email Content Service, visit http://www.verizonbusiness.com. ______________________________________________________________________ From marktarango <@t> gmail.com Mon Jan 6 17:03:14 2014 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Jan 6 17:03:21 2014 Subject: [Histonet] NCCI interpretation for ISH coding In-Reply-To: <6D7752544B308D44A902C0BD0EC7BF5C14EA03275D@EXCH02.wellspan.org> References: <6D7752544B308D44A902C0BD0EC7BF5C14EA03275D@EXCH02.wellspan.org> Message-ID: My understanding was that this is just for Medicare patients... On Mon, Jan 6, 2014 at 12:35 PM, Johns, Jill wrote: > The following was taken from the NCCI manual, Chapter 10, effective 1/1/14: > > 9. The unit of service for in situ hybridization reported as CPT codes > 88365, 88367, or 88368 is each probe staining procedure per specimen. If a > single probe staining procedure for one or more probes is performed on > multiple blocks from a surgical specimen, multiple slides from a cytologic > specimen, or multiple slides from a hematologic specimen, only one unit of > service may be reported for each separate specimen. Physicians should not > report more than one unit of service for CPT codes 88365, 88367, or 88368 > per specimen for a probe staining procedure even if it contains multiple > separately interpretable probes. > > I'm wondering how other labs are interpreting this, because I'm not sure > how we continue to offer FISH testing and not go "in the hole" financially, > if we can only bill for ONE probe on ONE specimen, regardless of the actual > number of interpretable probes (Example: currently, for a HER2 FISH test, > we bill for 2 units of service--1 for the HER2 probe and 1 for the CEP17 > probe--both of which have to be enumerated by a professional and a ratio > generated). Any insight from others would be greatly appreciated....Thanks! > > > Jill A. Johns, MT(ASCP)SH, QCym, CCy > Manager of Molecular Pathology > Central PA Alliance Laboratory (CPAL) > 1803 Mt. Rose Ave., Suite C3/C4 > York, PA 17403 > phone: (717) 851-4320 > fax: (717) 851-1450 > email: jjohns3@cpallab.com > > > ________________________________ > CONFIDENTIALITY NOTICE: > > This email may contain confidential health information that is legally > privileged. This information is intended for the use of the named > recipient(s). The authorized recipient of this information is prohibited > from disclosing this information to any party unless required to do so by > law or regulation and is required to destroy the information after its > stated need has been fulfilled. If you are not the intended recipient, you > are hereby notified that any disclosure, copying, distribution, or action > taken in reliance on the contents of this email is strictly prohibited. If > you receive this e-mail message in error, please notify the sender > immediately to arrange disposition of the information.. > > ______________________________________________________________________ > This e-mail has been scanned by Verizon Managed Email Content Service, > using Skeptic(tm) technology powered by MessageLabs. For more information > on Verizon's Managed Email Content Service, visit > http://www.verizonbusiness.com. > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amber.mckenzie <@t> gastrodocs.net Tue Jan 7 08:35:13 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jan 7 08:35:17 2014 Subject: [Histonet] Safety items In-Reply-To: <9A73AD69250B473CADD861FF7FE89595@HP2010> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> Message-ID: <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com> What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! From trathborne <@t> somerset-healthcare.com Tue Jan 7 08:52:55 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 7 08:53:19 2014 Subject: [Histonet] RE: Safety items In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95AE796@SMCMAIL01.somerset-healthcare.com> Safety glasses, counter top shield, lab coats. A fire blanket is another safety measure you could look into. Be sure that sprinklers are tested periodically, and that all flammables are in a safety cabinet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LMurphy2 <@t> aultman.com Tue Jan 7 09:07:01 2014 From: LMurphy2 <@t> aultman.com (Leann M. Murphy) Date: Tue Jan 7 09:07:10 2014 Subject: [Histonet] IHC CPT codes Message-ID: Good morning, I know that this has been talked about on here before but I cannot find my email regarding this issue. I need your help. When billing for IHC is the cpt code 88343 pertaining to the dual or triple antibodies on one slide? 88342 for the initial antibody? 88434 for each additional antibody? Thx. LeAnn Murphy, HT (ASCP) Aultman Hospital Canton, Ohio From amurvosh <@t> advancederm.net Tue Jan 7 09:25:56 2014 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Tue Jan 7 09:26:12 2014 Subject: [Histonet] Safety items In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com> Message-ID: <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmendell <@t> goldbergmd.net Tue Jan 7 09:31:00 2014 From: kmendell <@t> goldbergmd.net (MaryK Mendell) Date: Tue Jan 7 09:31:09 2014 Subject: [Histonet] Safety items In-Reply-To: <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com>, <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> Message-ID: <8F7FA50134ED99419B6CD9A978CDD7A55619A1A8@EXMBX104A.mmeprod.cbeyond> also you should have posted the emergency evacuation map as well meeting place. Kate Mendell Histopathology/Lab Manager Anyone who stops learning is old, whether at twenty or eighty. ~Henry Ford HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmendell@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurvosh@advancederm.net] Sent: Tuesday, January 07, 2014 10:25 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert-Eytalis <@t> RiversideHealthCare.net Tue Jan 7 09:50:53 2014 From: Robert-Eytalis <@t> RiversideHealthCare.net (Eytalis, Robert A) Date: Tue Jan 7 09:51:02 2014 Subject: [Histonet] Safety items In-Reply-To: <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com>, <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> Message-ID: Newest CAP inspection stupid thing. They want you to periodically check your spill kit for clumping, since it does not have an expiration date. I challenged it, but don't know if will go through. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurvosh@advancederm.net] Sent: Tuesday, January 07, 2014 9:25 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Jan 7 10:08:51 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Jan 7 10:09:09 2014 Subject: [Histonet] Safety items In-Reply-To: References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com>, <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95AE7F4@SMCMAIL01.somerset-healthcare.com> Are they looking for documentation that it's been checked, or will they take your word for it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Tuesday, January 07, 2014 10:51 AM To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Newest CAP inspection stupid thing. They want you to periodically check your spill kit for clumping, since it does not have an expiration date. I challenged it, but don't know if will go through. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurvosh@advancederm.net] Sent: Tuesday, January 07, 2014 9:25 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Fawn.Bomar <@t> HalifaxRegional.com Tue Jan 7 10:23:51 2014 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Tue Jan 7 10:24:03 2014 Subject: [Histonet] Frozen section TAT Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B96D91F5@EXCH-2K10.hrhs.com> Hi Everyone, Hope you all had a wonderful Christmas and a Happy New Year!! I am working on my Quality Indicators/Assessment and was setting a goal for our frozen section TAT's and was wondering if there was an overall standard TAT goal that everyone was using as far as percentage of frozens reported within 20 minutes. (i.e., goal of less than 1% outside of 20 minutes or goal of 90% being completed within 20 minutes). Also, do you all record the time that you give the pathologist the slide as the end time or the time the pathologist reports the results as the end time? Thank you in advance Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From lblazek <@t> digestivespecialists.com Tue Jan 7 10:25:57 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jan 7 10:26:02 2014 Subject: [Histonet] Safety items In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95AE7F4@SMCMAIL01.somerset-healthcare.com> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com>, <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> <3AD061FE740D464FAC7BF6B5CFB75707A95AE7F4@SMCMAIL01.somerset-healthcare.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39167A00448F@IBMB7Exchange.digestivespecialists.com> If it isn't documented it isn't done! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 07, 2014 11:09 AM To: 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Are they looking for documentation that it's been checked, or will they take your word for it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Tuesday, January 07, 2014 10:51 AM To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Newest CAP inspection stupid thing. They want you to periodically check your spill kit for clumping, since it does not have an expiration date. I challenged it, but don't know if will go through. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurvosh@advancederm.net] Sent: Tuesday, January 07, 2014 9:25 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Jan 7 10:44:03 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 7 10:45:29 2014 Subject: [Histonet] AW:cont. workflow Message-ID: <000601cf0bc7$acbeda20$063c8e60$@gmx.at> Hi Peggy! You talk about a lab with many specimens, many blocks. What do you think is the number of blocks, where a change from "batch" to "flow" makes sense? We have about 300 blocks per day and work from 6.30 till 15.30. Usually we are ready with slide-distribution at noon. And we do also IHC, FISH, mutation analysis, IF , many fast frozens- so we are busy, when we are not dealing with routine-histology. And we are happy with our system. ;-) Fixation is all in a comparable range. TAT of biopsies is one day after entry (patients are called in usually one week afterwards from the clinicians...). And we have a very diversified workplace with comfortable workshifts. Advisers often hold up cont. workflow as best, but I think it must fit to the circumstances! Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Lee & Peggy Wenk Gesendet: Montag, 06. J?nner 2014 18:31 An: Podawiltz, Thomas; Deanna Leslie; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] Soaking artifact In most labs, the processor runs all night long. Someone comes in very early in the morning, empties the processor, starts the purge cycle, and then starts embedding a lot of blocks. The tissue processor then sits, doing nothing, from after the purge in the early morning, until sometime in the late afternoon, when all the tissues are loaded in for the overnight run. That means the tissue processor is doing nothing for up to 12 hours during the daytime. How about, besides the overnight run, we can set up 1 or 2 other shorter runs during the day, with the small biopsies. How about - process all the large tissues overnight, but keep the little biopsies that you grossed all afternoon in formalin until the morning. Empty out the large tissues, purge the process, embed the large tissues and start microtoming them. After the purge is done, put the small biopsies from the afternoon on the tissue processor, and process them for 1.5-2 hours (and if your processor is able to process half a load, do that to save on reagents). Then embed them and start microtoming them. Purge the processor again. In the mean time, all morning, collect the small biopsies again. After lunch, short process all the small biopsies from the morning. Embed them in the afternoon, purge the processor again, and load up the overnight load. If you don't have time to microtome the morning small biopsies (that you embedded in the afternoon), someone can microtome them in the morning the next day. Either with the large overnight load, or have someone else come in early, and while the other people are embedding the large tissue overnight load, they can be microtoming the small biopsies that were embedded in previous afternoon. Yes, all of this will mean changes: - staggered hours that people will be coming in - processing, embedding and microtoming continuously throughout the day - someone might have to microtome more than someone else, or might have to embed more than someone else. But if you rotate jobs around, over the months, everyone ends up doing the same amount of work overall. This is a type of continuous work flow, and does lead to faster turn around time and efficiency. When our lab changed to this system (we are an 1100 bed hospital, with lots of tissues from our ORs, from outside hospitals and clinics and doctors offices, so lots and lots of blocks), it took getting everyone involved - people accessioning, grossing, the histotechs, and the pathologists (they were not going to get their slides in numerical order). We have short cycles, the overnight long cycles, some rush cycles, and long cycles for breast and autopsy brain. We actually have more than 3 runs, but then we are working almost 24/7. During the time we were switching to continuous work flow, we had a few histotechs off on maternity and/or medical leaves. And we got a couple more clients, so the work load increased. But because of the continuous work flow, we were able to handle the additional work without having to hire anyone. Whereas before, we would process ALL the blocks overnight, and then would have lots of people embedding lots of blocks first thing in the very early morning, and then having to put in the in order, and no one could start microtoming until all the blocks were embedding and in order (so some days the microtoming techs were sitting around with nothing to do for a time). Then, everyone had piles of blocks to cut, for blocks 100-150 were going to be cut hours and hours after everyone started microtoming blocks 1-50. Then, there were racks and racks of slide piling up to be stained with H&E (and at that point, we were still labeling after staining). So there were all these spots where tissue was being held up, unnecessarily: - waiting to be processed - waiting to be embedding - waiting to be microtomed - waiting to be stained - waiting to be labeled - then the pathologists had stacks of slides, waiting to be diagnosed - waiting for the reports to be typed With continuous flow, there is always some work coming through, but not large piles causing long waits. It just takes rethinking how you can use the processor more efficiently, which will make the rest of the work more efficient. And increase productivity and make turn around time faster. Peggy A. Wenk, HTL(ASCP) -----Original Message----- From: Podawiltz, Thomas Sent: Monday, January 06, 2014 12:04 PM To: Lee & Peggy Wenk ; Deanna Leslie ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Soaking artifact I agree Peggy. Just one question. What is a small histology lab to do when they only have one processor and cannot run separate cycles and do not have staffing to run short cycles throughout the day? Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, January 06, 2014 8:07 AM To: Deanna Leslie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Soaking artifact The only "soaking" artifacts that I can think of would be caused by: - soaking too long in water (minutes instead of a few seconds) - soaking under-processed tissue in water In both cases, the tissue is supposed to be "protected" by the wax, and if it is not (under-processed), or if the faced block is in water too long, the tissue can start re-absorbing water. The tissue then turns white and swells out of the block. So all that "swelled out" tissue is cut away and lost when the tissue is put back on the microtomy for sectioning the ribbon. If you soak for just a few seconds, such as a gauze with water being held against the block on the microtome, after it has been faced, then you will get a little bit of water absorbed into just a few layers of cells. Just enough to cut 2-4 sections. And you won't see that swelling artifact. For those of you saying - "but I have to face all the blocks, put them back on ice and/or water while I cut a bunch more blocks, and then go back and cut each block" - that is an artifact also. You have over-dehydrated your tissue during processing, and you are putting back the water that you should not have taken out. Processing is supposed to remove the unbound water (not attached to proteins), and some of the bound water (attached to proteins), and leave some of the bound water (attached to proteins) in the tissue. If you HAVE to soak EVERY block for more than a couple of seconds, then you are wasting time rehydrating and wasting time while microtoming. Cut down the time in the alcohols on the tissue processor, to leave a little bound water in the tissues. And you can NOT processing little biopsies on the same long processing cycle as the larger pieces of tissue (uterus, breast, etc.). Those little biopsies will be over-dehydrated. They HAVE to be run on a separate cycle of much shorter time intervals (10-20 minutes in each solution (once fixed), instead of 45-60 minutes in each solution). You should be able (on nearly every tissue block) to rough trim the tissue, and immediately start cutting ribbons. Possibly, you will need to put an ice cube on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the paraffin hardness to match the hardness of the tissue. That being said, some tissues are naturally brittle or crumbly, and always need some water put back in the tissue, such as spleen or bloody tissue, but again, some wet gauze on the faced block for a few seconds should be enough time to get 2-4 sections. And that's all the tissue we usually need from those blocks. If you need more for IHC, put the wet gauze back on the faced block, and cut a few more sections. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Deanna Leslie Sent: Sunday, January 05, 2014 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Tue Jan 7 10:55:31 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Jan 7 10:55:40 2014 Subject: [Histonet] Safety items In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39167A00448F@IBMB7Exchange.digestivespecialists.com> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com>, <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> <3AD061FE740D464FAC7BF6B5CFB75707A95AE7F4@SMCMAIL01.somerset-healthcare.com> <5A2BD13465E061429D6455C8D6B40E39167A00448F@IBMB7Exchange.digestivespecialists.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F64FF18040@GHSEXMBX1W8K1V.geisinger.edu> That should be the slogan for NSH next year :D -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, January 07, 2014 11:26 AM To: Rathborne, Toni; 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items If it isn't documented it isn't done! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 07, 2014 11:09 AM To: 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Are they looking for documentation that it's been checked, or will they take your word for it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Tuesday, January 07, 2014 10:51 AM To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Newest CAP inspection stupid thing. They want you to periodically check your spill kit for clumping, since it does not have an expiration date. I challenged it, but don't know if will go through. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurvosh@advancederm.net] Sent: Tuesday, January 07, 2014 9:25 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From akbitting <@t> geisinger.edu Tue Jan 7 10:56:20 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Jan 7 10:56:25 2014 Subject: [Histonet] NCCI interpretation for ISH coding In-Reply-To: References: <6D7752544B308D44A902C0BD0EC7BF5C14EA03275D@EXCH02.wellspan.org> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F64FF18067@GHSEXMBX1W8K1V.geisinger.edu> That's my understanding as well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Tarango Sent: Monday, January 06, 2014 6:03 PM To: Johns, Jill Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NCCI interpretation for ISH coding My understanding was that this is just for Medicare patients... On Mon, Jan 6, 2014 at 12:35 PM, Johns, Jill wrote: > The following was taken from the NCCI manual, Chapter 10, effective 1/1/14: > > 9. The unit of service for in situ hybridization reported as CPT codes > 88365, 88367, or 88368 is each probe staining procedure per specimen. > If a single probe staining procedure for one or more probes is > performed on multiple blocks from a surgical specimen, multiple slides > from a cytologic specimen, or multiple slides from a hematologic > specimen, only one unit of service may be reported for each separate > specimen. Physicians should not report more than one unit of service > for CPT codes 88365, 88367, or 88368 per specimen for a probe staining > procedure even if it contains multiple separately interpretable probes. > > I'm wondering how other labs are interpreting this, because I'm not > sure how we continue to offer FISH testing and not go "in the hole" > financially, if we can only bill for ONE probe on ONE specimen, > regardless of the actual number of interpretable probes (Example: > currently, for a HER2 FISH test, we bill for 2 units of service--1 for > the HER2 probe and 1 for the CEP17 probe--both of which have to be > enumerated by a professional and a ratio generated). Any insight from others would be greatly appreciated....Thanks! > > > Jill A. Johns, MT(ASCP)SH, QCym, CCy > Manager of Molecular Pathology > Central PA Alliance Laboratory (CPAL) > 1803 Mt. Rose Ave., Suite C3/C4 > York, PA 17403 > phone: (717) 851-4320 > fax: (717) 851-1450 > email: jjohns3@cpallab.com > > > ________________________________ > CONFIDENTIALITY NOTICE: > > This email may contain confidential health information that is legally > privileged. This information is intended for the use of the named > recipient(s). The authorized recipient of this information is > prohibited from disclosing this information to any party unless > required to do so by law or regulation and is required to destroy the > information after its stated need has been fulfilled. If you are not > the intended recipient, you are hereby notified that any disclosure, > copying, distribution, or action taken in reliance on the contents of > this email is strictly prohibited. If you receive this e-mail message > in error, please notify the sender immediately to arrange disposition of the information.. > > ______________________________________________________________________ > This e-mail has been scanned by Verizon Managed Email Content Service, > using Skeptic(tm) technology powered by MessageLabs. For more > information on Verizon's Managed Email Content Service, visit > http://www.verizonbusiness.com. > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From c.tague <@t> Pathologyarts.com Tue Jan 7 12:33:26 2014 From: c.tague <@t> Pathologyarts.com (Curt) Date: Tue Jan 7 11:26:20 2014 Subject: [Histonet] slide labeling Message-ID: <9C8F910F72893643B3C3793C3D67132B013FEE89@PATHOLOGYSERVER.pathologyarts.local> So we have a facility we do some work for that requires both the hand written number and the slide label be viewable, so we place the slide label a little lower and write the number on the top of the frosted end. Does CAP recognize both numbers as a legal record? The reason I ask is because there was a case that the hand written number was wrong but the slide label was correct. That being said, there's still a QA issue with the slide not being accurately labeled. Slides are labeled at microtomy too, the hand written number and the slide label. I contend internally that the slide WAS accurately labeled because the sticker number and block number correspond accurately, the discrepancy was in the hand written number. Thoughts? Does CAP have any guidance on this? Thanks, Curt From vrivera <@t> westderm.com Tue Jan 7 11:30:30 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Tue Jan 7 11:30:37 2014 Subject: [Histonet] Safety items In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95AE7F4@SMCMAIL01.somerset-healthcare.com> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com>, <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> <3AD061FE740D464FAC7BF6B5CFB75707A95AE7F4@SMCMAIL01.somerset-healthcare.com> Message-ID: <3D4A471B82E7A44C87F6839732320D9F041621@VSPDMS-ITEXMB02.DMS.COM> Document, document, document! Believe me, you will get dinged on this if it is not documented, we found out first hand :( Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 07, 2014 8:09 AM To: 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Are they looking for documentation that it's been checked, or will they take your word for it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Tuesday, January 07, 2014 10:51 AM To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Newest CAP inspection stupid thing. They want you to periodically check your spill kit for clumping, since it does not have an expiration date. I challenged it, but don't know if will go through. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurvosh@advancederm.net] Sent: Tuesday, January 07, 2014 9:25 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Tue Jan 7 11:39:29 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jan 7 11:39:34 2014 Subject: [Histonet] Spill kit In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F041621@VSPDMS-ITEXMB02.DMS.COM> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com>, <4AD6A4E531E8C943A730559B6B81DF07CB939E@dc.Advancederm.net> <3AD061FE740D464FAC7BF6B5CFB75707A95AE7F4@SMCMAIL01.somerset-healthcare.com> <3D4A471B82E7A44C87F6839732320D9F041621@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0112440716@JERRY.Gia.com> Where do you get your spill kit? Do you have a company and part number? Thanks! -----Original Message----- From: Vincent Rivera [mailto:vrivera@westderm.com] Sent: Tuesday, January 07, 2014 11:31 AM To: 'Rathborne, Toni'; 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Document, document, document! Believe me, you will get dinged on this if it is not documented, we found out first hand :( Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 07, 2014 8:09 AM To: 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Are they looking for documentation that it's been checked, or will they take your word for it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Tuesday, January 07, 2014 10:51 AM To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Newest CAP inspection stupid thing. They want you to periodically check your spill kit for clumping, since it does not have an expiration date. I challenged it, but don't know if will go through. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurvosh@advancederm.net] Sent: Tuesday, January 07, 2014 9:25 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cathy.crumpton <@t> tuality.org Tue Jan 7 12:07:19 2014 From: cathy.crumpton <@t> tuality.org (Cathy Crumpton) Date: Tue Jan 7 12:07:26 2014 Subject: [Histonet] Part time histotech needed in OR Message-ID: We just posted a part time HT position at Tuality Community Hospital in Hillsboro, Oregon. Go to Tuality.org under careers to apply. Tuality has a great pathology lab that is only open M-F. Position is for 24 hours per week with benefits. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 From tbraud <@t> holyredeemer.com Tue Jan 7 12:11:58 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Jan 7 12:12:03 2014 Subject: [Histonet] Safety Items In-Reply-To: <20140107165548.80DD91E8068@trendmess-svr.holyredeemer.local> References: <20140107165548.80DD91E8068@trendmess-svr.holyredeemer.local> Message-ID: On Tue, 7 Jan 2014, Amber asked: What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! We also keep: 1. Full face splash shields 2. Masks 3. Full sleeve, fluid resistant lab coats (OSHA requirement) 4. Plastic/Rubber Aprons 5. Chemical proof safety goggles 6. Spill kits appropriate for bases, acids, and formalin (and mercury if still used) 7. Spill containment systems appropriate for 5 gal carboys of Formalin (in case of leak) 8. Cut proof/resistant gloves in all appropriate sizes 9. Regular Gloves, chemical resistant grade (latex is not appropriate for formalin, we use Chemical resistant nitrile) 10. Fire Extinguishers 11. Shoe covers and hair covers 12. Thermo gloves if handling liquid nitrogen or dry ice 13. Sharps containers 14. One handed scalpel removal/replacement system (OSHA Requirement) 15. Safety scalpels with retractable covers. 16. Disposable dissecting knives 10" 17. Flammable Cabinet. 18. Acid Proof Cabinet 19. Posted Evacuation Routes (2 per) 20. Fume Hood 21. Chemical Waste storage containers Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 4 Date: Tue, 7 Jan 2014 14:35:13 +0000 From: Amber McKenzie Subject: [Histonet] Safety items To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BC011244050B@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! **************************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. 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From Dorothy.L.Webb <@t> HealthPartners.Com Tue Jan 7 12:14:45 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Jan 7 12:17:22 2014 Subject: [Histonet] spill kits In-Reply-To: References: Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E75B@HPEMX3.HealthPartners.int> You can go through any company pretty much, but remember you need a formalin spill kit as well as a solvent spill kit (for xylene and other spills) Dorothy Webb -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, January 07, 2014 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 122, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Spill kit (Amber McKenzie) ---------------------------------------------------------------------- Message: 1 Date: Tue, 7 Jan 2014 17:39:29 +0000 From: Amber McKenzie Subject: [Histonet] Spill kit To: Vincent Rivera , "'Rathborne, Toni'" , "'Eytalis, Robert A'" , "Anne Murvosh" , "histonet@lists.utsouthwestern.edu" Message-ID: <5A33C952BB67F4468AF1F36D739212BC0112440716@JERRY.Gia.com> Content-Type: text/plain; charset="us-ascii" Where do you get your spill kit? Do you have a company and part number? Thanks! -----Original Message----- From: Vincent Rivera [mailto:vrivera@westderm.com] Sent: Tuesday, January 07, 2014 11:31 AM To: 'Rathborne, Toni'; 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Document, document, document! Believe me, you will get dinged on this if it is not documented, we found out first hand :( Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 07, 2014 8:09 AM To: 'Eytalis, Robert A'; Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Are they looking for documentation that it's been checked, or will they take your word for it? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Tuesday, January 07, 2014 10:51 AM To: Anne Murvosh; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Newest CAP inspection stupid thing. They want you to periodically check your spill kit for clumping, since it does not have an expiration date. I challenged it, but don't know if will go through. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurvosh@advancederm.net] Sent: Tuesday, January 07, 2014 9:25 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Safety items Don't forget to make sure you have a spill kit and its labeled well with easy access, Inspectors will ask employees if they know where it and your fire extinguisher and alarm are located. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety items What safety items do you keep in your lab besides the normal apron, gloves, eye wash stations and shower? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 122, Issue 8 **************************************** This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. 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Disclaimer R001.0 From c.tague <@t> Pathologyarts.com Tue Jan 7 13:27:35 2014 From: c.tague <@t> Pathologyarts.com (Curt) Date: Tue Jan 7 12:20:27 2014 Subject: [Histonet] RE: slide labeling In-Reply-To: <9C8F910F72893643B3C3793C3D67132B013FEE89@PATHOLOGYSERVER.pathologyarts.local> References: <9C8F910F72893643B3C3793C3D67132B013FEE89@PATHOLOGYSERVER.pathologyarts.local> Message-ID: <9C8F910F72893643B3C3793C3D67132B013FF0AB@PATHOLOGYSERVER.pathologyarts.local> To add clarity, there is a 2D barcode on the block, we scan at microtomy and then label the slide. The system is really idiot proof "ASSUMING" the techs cut and label one block at a time, which we strictly enforce. The error here occurred when writing the number on the slide, I think he looked at the wrong block here, so in effect he did label a number of slides at the same time but the slide label was already affixed to the slide. I guess the rule should be to write the number as it's seen on the slide. Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Sent: Tuesday, January 07, 2014 10:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide labeling So we have a facility we do some work for that requires both the hand written number and the slide label be viewable, so we place the slide label a little lower and write the number on the top of the frosted end. Does CAP recognize both numbers as a legal record? The reason I ask is because there was a case that the hand written number was wrong but the slide label was correct. That being said, there's still a QA issue with the slide not being accurately labeled. Slides are labeled at microtomy too, the hand written number and the slide label. I contend internally that the slide WAS accurately labeled because the sticker number and block number correspond accurately, the discrepancy was in the hand written number. Thoughts? Does CAP have any guidance on this? Thanks, Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Tue Jan 7 12:27:48 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Tue Jan 7 12:27:56 2014 Subject: [Histonet] AW:cont. workflow In-Reply-To: <000601cf0bc7$acbeda20$063c8e60$@gmx.at> References: <000601cf0bc7$acbeda20$063c8e60$@gmx.at> Message-ID: <496b19ea2153420cb742f2a00628c218@BL2PR04MB196.namprd04.prod.outlook.com> I'm curious. How many techs do you have working in your lab that you are able to complete 300 blocks including IHC, FISH, etc in 9 hours? Do your techs also gross? Do your techs put the slides together in folders with reports to be distributed to the pathologists? Thanks, Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gudrun Lang Sent: Tuesday, January 07, 2014 2:44 PM To: 'Lee & Peggy Wenk' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] AW:cont. workflow Hi Peggy! You talk about a lab with many specimens, many blocks. What do you think is the number of blocks, where a change from "batch" to "flow" makes sense? We have about 300 blocks per day and work from 6.30 till 15.30. Usually we are ready with slide-distribution at noon. And we do also IHC, FISH, mutation analysis, IF , many fast frozens- so we are busy, when we are not dealing with routine-histology. And we are happy with our system. ;-) Fixation is all in a comparable range. TAT of biopsies is one day after entry (patients are called in usually one week afterwards from the clinicians...). And we have a very diversified workplace with comfortable workshifts. Advisers often hold up cont. workflow as best, but I think it must fit to the circumstances! Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Lee & Peggy Wenk Gesendet: Montag, 06. J?nner 2014 18:31 An: Podawiltz, Thomas; Deanna Leslie; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] Soaking artifact In most labs, the processor runs all night long. Someone comes in very early in the morning, empties the processor, starts the purge cycle, and then starts embedding a lot of blocks. The tissue processor then sits, doing nothing, from after the purge in the early morning, until sometime in the late afternoon, when all the tissues are loaded in for the overnight run. That means the tissue processor is doing nothing for up to 12 hours during the daytime. How about, besides the overnight run, we can set up 1 or 2 other shorter runs during the day, with the small biopsies. How about - process all the large tissues overnight, but keep the little biopsies that you grossed all afternoon in formalin until the morning. Empty out the large tissues, purge the process, embed the large tissues and start microtoming them. After the purge is done, put the small biopsies from the afternoon on the tissue processor, and process them for 1.5-2 hours (and if your processor is able to process half a load, do that to save on reagents). Then embed them and start microtoming them. Purge the processor again. In the mean time, all morning, collect the small biopsies again. After lunch, short process all the small biopsies from the morning. Embed them in the afternoon, purge the processor again, and load up the overnight load. If you don't have time to microtome the morning small biopsies (that you embedded in the afternoon), someone can microtome them in the morning the next day. Either with the large overnight load, or have someone else come in early, and while the other people are embedding the large tissue overnight load, they can be microtoming the small biopsies that were embedded in previous afternoon. Yes, all of this will mean changes: - staggered hours that people will be coming in - processing, embedding and microtoming continuously throughout the day - someone might have to microtome more than someone else, or might have to embed more than someone else. But if you rotate jobs around, over the months, everyone ends up doing the same amount of work overall. This is a type of continuous work flow, and does lead to faster turn around time and efficiency. When our lab changed to this system (we are an 1100 bed hospital, with lots of tissues from our ORs, from outside hospitals and clinics and doctors offices, so lots and lots of blocks), it took getting everyone involved - people accessioning, grossing, the histotechs, and the pathologists (they were not going to get their slides in numerical order). We have short cycles, the overnight long cycles, some rush cycles, and long cycles for breast and autopsy brain. We actually have more than 3 runs, but then we are working almost 24/7. During the time we were switching to continuous work flow, we had a few histotechs off on maternity and/or medical leaves. And we got a couple more clients, so the work load increased. But because of the continuous work flow, we were able to handle the additional work without having to hire anyone. Whereas before, we would process ALL the blocks overnight, and then would have lots of people embedding lots of blocks first thing in the very early morning, and then having to put in the in order, and no one could start microtoming until all the blocks were embedding and in order (so some days the microtoming techs were sitting around with nothing to do for a time). Then, everyone had piles of blocks to cut, for blocks 100-150 were going to be cut hours and hours after everyone started microtoming blocks 1-50. Then, there were racks and racks of slide piling up to be stained with H&E (and at that point, we were still labeling after staining). So there were all these spots where tissue was being held up, unnecessarily: - waiting to be processed - waiting to be embedding - waiting to be microtomed - waiting to be stained - waiting to be labeled - then the pathologists had stacks of slides, waiting to be diagnosed - waiting for the reports to be typed With continuous flow, there is always some work coming through, but not large piles causing long waits. It just takes rethinking how you can use the processor more efficiently, which will make the rest of the work more efficient. And increase productivity and make turn around time faster. Peggy A. Wenk, HTL(ASCP) -----Original Message----- From: Podawiltz, Thomas Sent: Monday, January 06, 2014 12:04 PM To: Lee & Peggy Wenk ; Deanna Leslie ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Soaking artifact I agree Peggy. Just one question. What is a small histology lab to do when they only have one processor and cannot run separate cycles and do not have staffing to run short cycles throughout the day? Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, January 06, 2014 8:07 AM To: Deanna Leslie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Soaking artifact The only "soaking" artifacts that I can think of would be caused by: - soaking too long in water (minutes instead of a few seconds) - soaking under-processed tissue in water In both cases, the tissue is supposed to be "protected" by the wax, and if it is not (under-processed), or if the faced block is in water too long, the tissue can start re-absorbing water. The tissue then turns white and swells out of the block. So all that "swelled out" tissue is cut away and lost when the tissue is put back on the microtomy for sectioning the ribbon. If you soak for just a few seconds, such as a gauze with water being held against the block on the microtome, after it has been faced, then you will get a little bit of water absorbed into just a few layers of cells. Just enough to cut 2-4 sections. And you won't see that swelling artifact. For those of you saying - "but I have to face all the blocks, put them back on ice and/or water while I cut a bunch more blocks, and then go back and cut each block" - that is an artifact also. You have over-dehydrated your tissue during processing, and you are putting back the water that you should not have taken out. Processing is supposed to remove the unbound water (not attached to proteins), and some of the bound water (attached to proteins), and leave some of the bound water (attached to proteins) in the tissue. If you HAVE to soak EVERY block for more than a couple of seconds, then you are wasting time rehydrating and wasting time while microtoming. Cut down the time in the alcohols on the tissue processor, to leave a little bound water in the tissues. And you can NOT processing little biopsies on the same long processing cycle as the larger pieces of tissue (uterus, breast, etc.). Those little biopsies will be over-dehydrated. They HAVE to be run on a separate cycle of much shorter time intervals (10-20 minutes in each solution (once fixed), instead of 45-60 minutes in each solution). You should be able (on nearly every tissue block) to rough trim the tissue, and immediately start cutting ribbons. Possibly, you will need to put an ice cube on some of the harder tissue (cervix, uterus, bone, lens, etc.) just to get the paraffin hardness to match the hardness of the tissue. That being said, some tissues are naturally brittle or crumbly, and always need some water put back in the tissue, such as spleen or bloody tissue, but again, some wet gauze on the faced block for a few seconds should be enough time to get 2-4 sections. And that's all the tissue we usually need from those blocks. If you need more for IHC, put the wet gauze back on the faced block, and cut a few more sections. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Deanna Leslie Sent: Sunday, January 05, 2014 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmweber113 <@t> comcast.net Tue Jan 7 12:55:32 2014 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Tue Jan 7 12:55:50 2014 Subject: Fwd: [Histonet] New code 88343 In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC181756C7@RRMBX03.rrmc.local> Message-ID: <1600457600.194339.1389120932345.JavaMail.root@sz0187a.westchester.pa.mail.comcast.net> ----- Forwarded Message ----- From: "Joe W. Walker, Jr." To: "Paula Lucas" , histonet@lists.utsouthwestern.edu Sent: Wednesday, December 18, 2013 4:15:39 PM Subject: RE: [Histonet] New code 88343 Yes, this appears to be correct. ?Excerpted from Dennis Padget: AMA prescribes codes 88342 and 88343 for qualitative IHC: The AMA's CPT-2014 codebook offers two codes for reporting with qualitative immunohistochemistry (IHC) testing. The codes and official parenthetical instructions for their use starting Jan. 1 are as follows: 88342 ?Immunohistochemistry or immunocytochemistry, each separately identifiable antibody per block, cytologic preparation, or hematologic smear; first separately identifiable antibody per slide ?? ? ? ? ? ?(Do not report 88342 in conjunction with 88360 or 88361 for the same antibody) ?? ? ? ? ? ?(For quantitative or semi-quantitative immunohistochemistry, see 88360, 88361) 88343 each additional separately identifiable antibody per slide (List separately in addition to code for primary procedure) ?? ? ?(Use 88343 in conjunction with 88342) ?? ? ?(When multiple antibodies are applied to the same slide, use one unit of 88342 for the first separately identifiable antibody and one unit of 88343 for each additional identifiable antibody) CMS prescribes codes G0461 and G0462 for qualitative IHC: CMS will not accept CPT code 88342 or 88343 on a claim effective with dates of service on and after Jan. 1, 2014: Those two codes are "not valid for Medicare purposes" and will be summarily denied if billed. To report a professional, technical or global charge for qualitative immunohistochemistry (IHC) testing for a Medicare beneficiary on and after Jan. 1, 2014, you must use the applicable HCPCS Level II code as follows: G0461 Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain G0462 ? ? ? each additional single or multiplex antibody stain (List separately in addition to code for primary procedure) Codes G0461 and G0462 have 26 and TC modifier lines in the 2014 physician fee schedule, so you'll bill them using the modifier (or no modifier, if entitled to bill the global service) that applies to your practice and any given Medicare beneficiary claim. CMS prescribes that you continue to bill for qualitative IHC testing 'per specimen' as you have since Jan. 1, 2012. Furthermore, you'll continue to bill for quantitative IHC testing 'per specimen' using CPT codes 88360 and 88361 just as you do today and have done since Jan. 1, 2012. Hope this helps. ?We are still working through how we are going to accomplish this in our LIS, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 ?F: 802.747.6525 Email joewalker@rrmc.org ? ?www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Tuesday, December 17, 2013 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New code 88343 Hello, Can someone shed some light for me on this new code for IHC? ?I think the code refers to double stain antibodies? Thanks in advance : ) Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Jan 7 14:11:37 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jan 7 14:11:49 2014 Subject: [Histonet] New code 88343 In-Reply-To: <1600457600.194339.1389120932345.JavaMail.root@sz0187a.westchester.pa.mail.comcast.net> References: <3C2378778400AD448ADA6FD6BDB7CCCC181756C7@RRMBX03.rrmc.local> <1600457600.194339.1389120932345.JavaMail.root@sz0187a.westchester.pa.mail.comcast.net> Message-ID: Does everyone charge non-Medicare per block or per specimen? Thanks, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rmweber113@comcast.net Sent: Tuesday, January 07, 2014 1:56 PM To: histonet Subject: Fwd: [Histonet] New code 88343 ----- Forwarded Message ----- From: "Joe W. Walker, Jr." To: "Paula Lucas" , histonet@lists.utsouthwestern.edu Sent: Wednesday, December 18, 2013 4:15:39 PM Subject: RE: [Histonet] New code 88343 Yes, this appears to be correct. Excerpted from Dennis Padget: AMA prescribes codes 88342 and 88343 for qualitative IHC: The AMA's CPT-2014 codebook offers two codes for reporting with qualitative immunohistochemistry (IHC) testing. The codes and official parenthetical instructions for their use starting Jan. 1 are as follows: 88342 Immunohistochemistry or immunocytochemistry, each separately identifiable antibody per block, cytologic preparation, or hematologic smear; first separately identifiable antibody per slide (Do not report 88342 in conjunction with 88360 or 88361 for the same antibody) (For quantitative or semi-quantitative immunohistochemistry, see 88360, 88361) 88343 each additional separately identifiable antibody per slide (List separately in addition to code for primary procedure) (Use 88343 in conjunction with 88342) (When multiple antibodies are applied to the same slide, use one unit of 88342 for the first separately identifiable antibody and one unit of 88343 for each additional identifiable antibody) CMS prescribes codes G0461 and G0462 for qualitative IHC: CMS will not accept CPT code 88342 or 88343 on a claim effective with dates of service on and after Jan. 1, 2014: Those two codes are "not valid for Medicare purposes" and will be summarily denied if billed. To report a professional, technical or global charge for qualitative immunohistochemistry (IHC) testing for a Medicare beneficiary on and after Jan. 1, 2014, you must use the applicable HCPCS Level II code as follows: G0461 Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain G0462 each additional single or multiplex antibody stain (List separately in addition to code for primary procedure) Codes G0461 and G0462 have 26 and TC modifier lines in the 2014 physician fee schedule, so you'll bill them using the modifier (or no modifier, if entitled to bill the global service) that applies to your practice and any given Medicare beneficiary claim. CMS prescribes that you continue to bill for qualitative IHC testing 'per specimen' as you have since Jan. 1, 2012. Furthermore, you'll continue to bill for quantitative IHC testing 'per specimen' using CPT codes 88360 and 88361 just as you do today and have done since Jan. 1, 2012. Hope this helps. We are still working through how we are going to accomplish this in our LIS, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Tuesday, December 17, 2013 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New code 88343 Hello, Can someone shed some light for me on this new code for IHC? I think the code refers to double stain antibodies? Thanks in advance : ) Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From linda555m <@t> yahoo.com Tue Jan 7 14:25:14 2014 From: linda555m <@t> yahoo.com (Linda Musto) Date: Tue Jan 7 14:25:19 2014 Subject: [Histonet] HPV Probes Message-ID: <1389126314.58310.YahooMailNeo@web120405.mail.ne1.yahoo.com> Good Afternoon, Since we can no longer get ASR Probes from Ventana for HPV testing on tissue, we are wondering what other labs are doing to provide this test. ??????????????? Your help is greatly appreciated ! ???????????????????? Linda From Caroline.Pratt <@t> uphs.upenn.edu Tue Jan 7 14:35:23 2014 From: Caroline.Pratt <@t> uphs.upenn.edu (Pratt, Caroline) Date: Tue Jan 7 14:35:29 2014 Subject: [Histonet] New code 88343 In-Reply-To: References: <3C2378778400AD448ADA6FD6BDB7CCCC181756C7@RRMBX03.rrmc.local> <1600457600.194339.1389120932345.JavaMail.root@sz0187a.westchester.pa.mail.comcast.net> Message-ID: <14D6A469D20B4F4AACC47C3E973C3FA8A56E7F@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Per specimen, but CAP is fighting for approval from CMS for per block billing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 07, 2014 3:12 PM To: histonet Subject: RE: [Histonet] New code 88343 Does everyone charge non-Medicare per block or per specimen? Thanks, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rmweber113@comcast.net Sent: Tuesday, January 07, 2014 1:56 PM To: histonet Subject: Fwd: [Histonet] New code 88343 ----- Forwarded Message ----- From: "Joe W. Walker, Jr." To: "Paula Lucas" , histonet@lists.utsouthwestern.edu Sent: Wednesday, December 18, 2013 4:15:39 PM Subject: RE: [Histonet] New code 88343 Yes, this appears to be correct. Excerpted from Dennis Padget: AMA prescribes codes 88342 and 88343 for qualitative IHC: The AMA's CPT-2014 codebook offers two codes for reporting with qualitative immunohistochemistry (IHC) testing. The codes and official parenthetical instructions for their use starting Jan. 1 are as follows: 88342 Immunohistochemistry or immunocytochemistry, each separately identifiable antibody per block, cytologic preparation, or hematologic smear; first separately identifiable antibody per slide (Do not report 88342 in conjunction with 88360 or 88361 for the same antibody) (For quantitative or semi-quantitative immunohistochemistry, see 88360, 88361) 88343 each additional separately identifiable antibody per slide (List separately in addition to code for primary procedure) (Use 88343 in conjunction with 88342) (When multiple antibodies are applied to the same slide, use one unit of 88342 for the first separately identifiable antibody and one unit of 88343 for each additional identifiable antibody) CMS prescribes codes G0461 and G0462 for qualitative IHC: CMS will not accept CPT code 88342 or 88343 on a claim effective with dates of service on and after Jan. 1, 2014: Those two codes are "not valid for Medicare purposes" and will be summarily denied if billed. To report a professional, technical or global charge for qualitative immunohistochemistry (IHC) testing for a Medicare beneficiary on and after Jan. 1, 2014, you must use the applicable HCPCS Level II code as follows: G0461 Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain G0462 each additional single or multiplex antibody stain (List separately in addition to code for primary procedure) Codes G0461 and G0462 have 26 and TC modifier lines in the 2014 physician fee schedule, so you'll bill them using the modifier (or no modifier, if entitled to bill the global service) that applies to your practice and any given Medicare beneficiary claim. CMS prescribes that you continue to bill for qualitative IHC testing 'per specimen' as you have since Jan. 1, 2012. Furthermore, you'll continue to bill for quantitative IHC testing 'per specimen' using CPT codes 88360 and 88361 just as you do today and have done since Jan. 1, 2012. Hope this helps. We are still working through how we are going to accomplish this in our LIS, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Tuesday, December 17, 2013 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New code 88343 Hello, Can someone shed some light for me on this new code for IHC? I think the code refers to double stain antibodies? Thanks in advance : ) Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From amber.mckenzie <@t> gastrodocs.net Tue Jan 7 15:31:03 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jan 7 15:31:08 2014 Subject: [Histonet] Acccessioning area/Grossing area In-Reply-To: <9A73AD69250B473CADD861FF7FE89595@HP2010> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> Message-ID: <5A33C952BB67F4468AF1F36D739212BC01124409D0@JERRY.Gia.com> Do you any of you have your accessioning area right next to your grossing station so that the person accessioning can put cases on a cart and the person grossing can just grab each case and gross it in as they are being accessioned? Just curious about this setup. Thanks! From mpence <@t> grhs.net Tue Jan 7 15:34:42 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jan 7 15:35:03 2014 Subject: [Histonet] RE: Acccessioning area/Grossing area In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC01124409D0@JERRY.Gia.com> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC01124409D0@JERRY.Gia.com> Message-ID: Yes. We have ours that way. Our whole lab "flows" around the room. This is the LEAN way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acccessioning area/Grossing area Do you any of you have your accessioning area right next to your grossing station so that the person accessioning can put cases on a cart and the person grossing can just grab each case and gross it in as they are being accessioned? Just curious about this setup. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Tue Jan 7 15:45:06 2014 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Tue Jan 7 15:45:11 2014 Subject: [Histonet] RE: Acccessioning area/Grossing area In-Reply-To: References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC01124409D0@JERRY.Gia.com> Message-ID: <8A2A8E67E35BB24DAD9375F9E16310752A36BFEF@MSGPEXCHA01A.mfad.mfroot.org> We do it that way as well. It seems to flow quite nicely and it works well for us. Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 07, 2014 3:35 PM To: 'Amber McKenzie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acccessioning area/Grossing area Yes. We have ours that way. Our whole lab "flows" around the room. This is the LEAN way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acccessioning area/Grossing area Do you any of you have your accessioning area right next to your grossing station so that the person accessioning can put cases on a cart and the person grossing can just grab each case and gross it in as they are being accessioned? Just curious about this setup. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From s_musat <@t> yahoo.com Wed Jan 8 00:37:53 2014 From: s_musat <@t> yahoo.com (Sorin Musat) Date: Wed Jan 8 00:38:04 2014 Subject: [Histonet] The Biopsy Chip for high throughput pathology in prostate cancer (and other organs, the list is open..) Message-ID: <1389163073.95509.YahooMailNeo@web161702.mail.bf1.yahoo.com> Dear Histoneters, I am a new subscriber to Histonet, albeit I've been following this great website for at least 10 years. I would like to post some information about a new medical device I invented and patented (the Biopsy Chip). This device was intended for increasing the efficiency of processing and sectioning prostate biopsies, but can be used for other organs that require multiple biopsies (breast, thyroid, skin, etc.,) as well as for friable tissues (bone marrow). For a quick preview, I uploaded on youtube a little movie clip we presented at The Romanian Annual Congress of Urology in 2011 (summarizing the results of our first pilot study): "High Throughput Biopsy Chip for the detection of Prostate Cancer": http://www.youtube.com/watch?v=zP1iiE3CEn0 After we patented and registered the medical device under the authority of the Romanian Ministry of Health, this technique was employed for over 2000 patients so far in a number of Romanian hospitals. We had very good results (significant economies in time and consumables + higher yield of tissue on the slides + more tissue left within the block + increased accuracy of the diagnostic). We already presented a couple of?abstracts?at 3 Urology conferences and we will publish very soon a full-length paper discussing our results. We believe that the Biopsy Chip is the first application of tissue microarrays in clinical diagnostic. Presently we are trying to find collaborators in order to test the Biopsy Chip for other organs/pathologies besides prostate cancer. We welcome any comments and suggestions. If anyone wants more information about this device I will be very happy to answer your questions. If appropriate, I could also upload a presentation on Histonet server. Sincerely Sorin Musat, MD, PhD THEMIS Pathology (formerly HistoBest Inc, Edmonton, AB, Canada) Bucharest, Romania mobile: (4)0768 735 194 / 0725 653 551 email:?s_musat@yahoo.com skype: sorin.musat2 From santij1 <@t> bellsouth.net Wed Jan 8 07:03:25 2014 From: santij1 <@t> bellsouth.net (Jerry Santiago) Date: Wed Jan 8 07:03:33 2014 Subject: [Histonet] Call for Abstracts References: Message-ID: <9A438A0D-DCCE-4557-890E-E10296D7E653@bellsouth.net> The Florida Society for Histotechnology is calling for abstracts for their 2014 meeting in Orlando, Florida. The meeting will take place May 15-18, 2014 at the Buena Vista Palace in Downtown Disney. We are looking for management, routine histology, special stains and immunohistochemistry presentations. > > Abstract submission information can be found on our website at www.fshgroup.org/meeting > From Kristen.Mcclendon <@t> stdavids.com Wed Jan 8 07:32:40 2014 From: Kristen.Mcclendon <@t> stdavids.com (Kristen.Mcclendon@stdavids.com) Date: Wed Jan 8 07:32:57 2014 Subject: [Histonet] RE: Acccessioning area/Grossing area In-Reply-To: <8A2A8E67E35BB24DAD9375F9E16310752A36BFEF@MSGPEXCHA01A.mfad.mfroot.org> References: <9A73AD69250B473CADD861FF7FE89595@HP2010> <5A33C952BB67F4468AF1F36D739212BC01124409D0@JERRY.Gia.com> <8A2A8E67E35BB24DAD9375F9E16310752A36BFEF@MSGPEXCHA01A.mfad.mfroot.org> Message-ID: <37BA7F1EFD11F940980547524273BBE4EF36948CF2@FWDCWPMSGCMS08.hca.corpad.net> We also do it this way and have for 30 years. It works well. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Tuesday, January 07, 2014 3:45 PM To: 'Mike Pence'; 'Amber McKenzie'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Acccessioning area/Grossing area Importance: Low We do it that way as well. It seems to flow quite nicely and it works well for us. Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 07, 2014 3:35 PM To: 'Amber McKenzie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Acccessioning area/Grossing area Yes. We have ours that way. Our whole lab "flows" around the room. This is the LEAN way. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, January 07, 2014 3:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acccessioning area/Grossing area Do you any of you have your accessioning area right next to your grossing station so that the person accessioning can put cases on a cart and the person grossing can just grab each case and gross it in as they are being accessioned? Just curious about this setup. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Wed Jan 8 08:19:20 2014 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Wed Jan 8 08:19:24 2014 Subject: [Histonet] Temporary position Message-ID: Hello, We are in search of a temporary histo-tech or histologist to work in our OTS Core lab. Primarily we do research work to support the efforts of the Cancer Center Investigators. A majority of the work is on animal tissues. Gross, Process, Embed, section and do recuts, i.e., lining up blocks. The lab also makes TMA's and coring paraffin blocks for RNA and DNA extractions. Please contact me if you are interested, or know someone that may be interested in this opportunity. Thanks, Helen 410.614.1660 http://tmalab.jhmi.edu/ http://prostatebiorepository.org/ From joelleweaver <@t> hotmail.com Wed Jan 8 08:28:03 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 8 08:28:07 2014 Subject: [Histonet] Acccessioning area/Grossing area In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC01124409D0@JERRY.Gia.com> References: , <9A73AD69250B473CADD861FF7FE89595@HP2010>, <5A33C952BB67F4468AF1F36D739212BC01124409D0@JERRY.Gia.com> Message-ID: Also going for "lean", 5s type of set up. The direction of travel of specimens aligns with the workflow, single piece. Use cart to move those cases complete & ready for gross to adjacent to gross station . Grossing person does not move with gloved hands to computer terminal area to get specimens, brought to them as ready with the cart, fully assembled, with verified requisition, paperwork and specimen containers. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: amber.mckenzie@gastrodocs.net > To: histonet@lists.utsouthwestern.edu > Date: Tue, 7 Jan 2014 21:31:03 +0000 > Subject: [Histonet] Acccessioning area/Grossing area > > > Do you any of you have your accessioning area right next to your grossing station so that the person accessioning can put cases on a cart and the person grossing can just grab each case and gross it in as they are being accessioned? Just curious about this setup. Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> texasheart.org Wed Jan 8 09:08:46 2014 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Wed Jan 8 09:08:50 2014 Subject: [Histonet] Leica 2135 parts Message-ID: Hi, I am looking for a flex shaft cable for our Leica microtome 2135. I have been told by Leica they no longer service or carry parts for this machine any longer. Ours is in excellent condition and a new one is not in our budget. Is there a Leica ?junkyard?? Thanks. Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. From lpwenk <@t> sbcglobal.net Wed Jan 8 10:38:22 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Jan 8 10:38:27 2014 Subject: [Histonet] Leica 2135 parts In-Reply-To: References: Message-ID: <6B5CCB42B73447E5B70F0EE469FFF836@HP2010> The following companies sell refurbished used histology equipment. They might have parts that they sell - I don?t know as I never asked them if they sell parts, but it's a place to start. (I've worked with both companies, buying used equipment for the School.) IMEB http://www.imebinc.com/ Rankin Biomedical http://www.rankinbiomed.com/ Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Molinari, Betsy Sent: Wednesday, January 08, 2014 10:08 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica 2135 parts Hi, I am looking for a flex shaft cable for our Leica microtome 2135. I have been told by Leica they no longer service or carry parts for this machine any longer. Ours is in excellent condition and a new one is not in our budget. Is there a Leica ?junkyard?? Thanks. Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From krisvale <@t> uab.edu Wed Jan 8 12:32:07 2014 From: krisvale <@t> uab.edu (Kristine B Valenteros) Date: Wed Jan 8 12:32:14 2014 Subject: [Histonet] Manual for Reichert-Jung Cryocut 1800 Message-ID: <058DAF41B84EF841881F34741F328C3B19DFE229@UABEXMB4.ad.uab.edu> Hello, Our lab is in need of a copy of the service manual for the Reichert-Jung Cryocut 1800. It was purchased refurbished and did not come with one. If anyone could forward a copy, we would greatly appreciate it! Thank you! Kristine (205) 996-7796 From BMolinari <@t> texasheart.org Wed Jan 8 12:57:45 2014 From: BMolinari <@t> texasheart.org (Molinari, Betsy) Date: Wed Jan 8 12:57:50 2014 Subject: [Histonet] Leica part, thank you Message-ID: Thank you for the responses. You have given me lots of leads. Sincerely, Betsy Betsy Molinari Senior Histology Research Technician 832-355-6524 | BMolinari@texasheart.org | www.texasheart.org 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute][THI News] [THI on Facebook] [THI on Flicker] [THI on Google] [THI on Pinterest] [THI on Twitter] [THI on You Tube] Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. From c.tague <@t> Pathologyarts.com Wed Jan 8 14:36:23 2014 From: c.tague <@t> Pathologyarts.com (Curt) Date: Wed Jan 8 13:29:15 2014 Subject: [Histonet] The Biopsy Chip for high throughput pathology in prostate cancer (and other organs, the list is open..) In-Reply-To: <1389163073.95509.YahooMailNeo@web161702.mail.bf1.yahoo.com> References: <1389163073.95509.YahooMailNeo@web161702.mail.bf1.yahoo.com> Message-ID: <9C8F910F72893643B3C3793C3D67132B013FFEB5@PATHOLOGYSERVER.pathologyarts.local> Interesting, are you having much success convincing the clinician to take the time to us the device? Looks like they might be required to be extremely careful as opposed to just shooting a core into a specimen jar. Also, how about specimen identy/location, are the rows numbered or something along those lines. Something unique about each row to differentiate one from the other? Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sorin Musat Sent: Tuesday, January 07, 2014 10:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] The Biopsy Chip for high throughput pathology in prostate cancer (and other organs, the list is open..) Dear Histoneters, I am a new subscriber to Histonet, albeit I've been following this great website for at least 10 years. I would like to post some information about a new medical device I invented and patented (the Biopsy Chip). This device was intended for increasing the efficiency of processing and sectioning prostate biopsies, but can be used for other organs that require multiple biopsies (breast, thyroid, skin, etc.,) as well as for friable tissues (bone marrow). For a quick preview, I uploaded on youtube a little movie clip we presented at The Romanian Annual Congress of Urology in 2011 (summarizing the results of our first pilot study): "High Throughput Biopsy Chip for the detection of Prostate Cancer": http://www.youtube.com/watch?v=zP1iiE3CEn0 After we patented and registered the medical device under the authority of the Romanian Ministry of Health, this technique was employed for over 2000 patients so far in a number of Romanian hospitals. We had very good results (significant economies in time and consumables + higher yield of tissue on the slides + more tissue left within the block + increased accuracy of the diagnostic). We already presented a couple of?abstracts?at 3 Urology conferences and we will publish very soon a full-length paper discussing our results. We believe that the Biopsy Chip is the first application of tissue microarrays in clinical diagnostic. Presently we are trying to find collaborators in order to test the Biopsy Chip for other organs/pathologies besides prostate cancer. We welcome any comments and suggestions. If anyone wants more information about this device I will be very happy to answer your questions. If appropriate, I could also upload a presentation on Histonet server. Sincerely Sorin Musat, MD, PhD THEMIS Pathology (formerly HistoBest Inc, Edmonton, AB, Canada) Bucharest, Romania mobile: (4)0768 735 194 / 0725 653 551 email:?s_musat@yahoo.com skype: sorin.musat2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From s_musat <@t> yahoo.com Wed Jan 8 14:26:57 2014 From: s_musat <@t> yahoo.com (Sorin Musat) Date: Wed Jan 8 14:27:02 2014 Subject: Fw: [Histonet] The Biopsy Chip for high throughput pathology in prostate cancer (and other organs, the list is open..) In-Reply-To: <1389212376.8788.YahooMailNeo@web161705.mail.bf1.yahoo.com> References: <1389163073.95509.YahooMailNeo@web161702.mail.bf1.yahoo.com> <9C8F910F72893643B3C3793C3D67132B013FFEB5@PATHOLOGYSERVER.pathologyarts.local> <1389212376.8788.YahooMailNeo@web161705.mail.bf1.yahoo.com> Message-ID: <1389212817.93005.YahooMailNeo@web161703.mail.bf1.yahoo.com> On Wednesday, January 8, 2014 10:19 PM, Sorin Musat wrote: Actually, it was easier than expected to convince the urologists to place the biopsies in the grooves of the chip (it did not take longer that the "traditional method" - I timed the first few patients and than it was an easy sell). Particularly since they were provided with a 2-D map of the prostate for them to mark the location of the individual biopsies. Of course there is a system of labeling the rows. The first one is dyed in a different color and/or there is a notch at the top left corner. Some people prefer chips with only 6 grooves (so they use one chip for the left lobe, the other one for the right lobe). Others even employ chips for 12 biopsies (one chip=one patient). The nice thing is that the biopsies keep the right orientation with regards to the entry point of the needle and one can easily draw some sort of 3-D representation of the cancer. We do not have here whole slide scanners and the software to do 3D imaging/reconstruction but it should be very easy. Also, even when the biopsy is coming out fragmented, the individual pieces of the biopsy will stay in place and not migrate. If you want, I can send you a presentation with real pictures and some stats on the first 1000 patients who benefited of the Biopsy Chip. A photograph is worth a thousand words. Sorin On Wednesday, January 8, 2014 9:29 PM, Curt wrote: Interesting, are you having much success convincing the clinician to take the time to us the device? Looks like they might be required to be extremely careful as opposed to just shooting a core into a specimen jar. Also, how about specimen identy/location, are the rows numbered or something along those lines. Something unique about each row to differentiate one from the other? Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sorin Musat Sent: Tuesday, January 07, 2014 10:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] The Biopsy Chip for high throughput pathology in prostate cancer (and other organs, the list is open..) Dear Histoneters, I am a new subscriber to Histonet, albeit I've been following this great website for at least 10 years. I would like to post some information about a new medical device I invented and patented (the Biopsy Chip). This device was intended for increasing the efficiency of processing and sectioning prostate biopsies, but can be used for other organs that require multiple biopsies (breast, thyroid, skin, etc.,) as well as for friable tissues (bone marrow). For a quick preview, I uploaded on youtube a little movie clip we presented at The Romanian Annual Congress of Urology in 2011 (summarizing the results of our first pilot study): "High Throughput Biopsy Chip for the detection of Prostate Cancer": http://www.youtube.com/watch?v=zP1iiE3CEn0 After we patented and registered the medical device under the authority of the Romanian Ministry of Health, this technique was employed for over 2000 patients so far in a number of Romanian hospitals. We had very good results (significant economies in time and consumables + higher yield of tissue on the slides + more tissue left within the block + increased accuracy of the diagnostic). We already presented a couple of?abstracts?at 3 Urology conferences and we will publish very soon a full-length paper discussing our results. We believe that the Biopsy Chip is the first application of tissue microarrays in clinical diagnostic. Presently we are trying to find collaborators in order to test the Biopsy Chip for other organs/pathologies besides prostate cancer. We welcome any comments and suggestions. If anyone wants more information about this device I will be very happy to answer your questions. If appropriate, I could also upload a presentation on Histonet server. Sincerely Sorin Musat, MD, PhD THEMIS Pathology (formerly HistoBest Inc, Edmonton, AB, Canada) Bucharest, Romania mobile: (4)0768 735 194 / 0725 653 551 email:?s_musat@yahoo.com skype: sorin.musat2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Jan 9 08:27:13 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jan 9 08:27:17 2014 Subject: [Histonet] slide labeling In-Reply-To: <9C8F910F72893643B3C3793C3D67132B013FEE89@PATHOLOGYSERVER.pathologyarts.local> References: <9C8F910F72893643B3C3793C3D67132B013FEE89@PATHOLOGYSERVER.pathologyarts.local> Message-ID: CAP has requirement for 2 identifiers on slides for intra-operative, such as frozen sections only as far I know. That's for slides since the case may not yet have an accession #. The blocks, 2 identifiers. For specimen integrity though, ( CAP required all over the place) you really want two different, unique identifiers on everything that you track through the whole process. I like your idea with the labeling, but the intent is to have two identifiers and to cross check them. I know how harsh the response can be sometimes when a "human" error ( such as failing to catch the discrepancy ) is made with an entirely flawed, and "human dependant" system. There is a tendancy to cast blame. I don't like using patient names on work product much personally, but with at least a partial manual system ( I have that too) it becomes necessary. I just flip the slide over and read what is under the label, match the name & number with the log, or LIS, and match the block with the section on the slide. Still not perfect. For ease of follow up- I just define what constitutes an "error", and how many you are "permitted" ( yes I know, its really none), # before there are consequences. BTW - CAP & NSH are working on a standard labeling guidelines for AP. It is in the "works" and defines a lot of these issues and more, I found it helpful. Not sure when it will be published. A draft is probably on CAP and NSH sites. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: c.tague@Pathologyarts.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 7 Jan 2014 18:33:26 +0000 > Subject: [Histonet] slide labeling > > So we have a facility we do some work for that requires both the hand written number and the slide label be viewable, so we place the slide label a little lower and write the number on the top of the frosted end. Does CAP recognize both numbers as a legal record? The reason I ask is because there was a case that the hand written number was wrong but the slide label was correct. That being said, there's still a QA issue with the slide not being accurately labeled. Slides are labeled at microtomy too, the hand written number and the slide label. I contend internally that the slide WAS accurately labeled because the sticker number and block number correspond accurately, the discrepancy was in the hand written number. > > Thoughts? Does CAP have any guidance on this? > > Thanks, > > Curt > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Thu Jan 9 08:56:57 2014 From: suetp918 <@t> comcast.net (Sue) Date: Thu Jan 9 08:57:25 2014 Subject: [Histonet] slide labeling In-Reply-To: Message-ID: <159032156.495849.1389279417012.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Joelle Check CAP questions again, I am going to as well.? We got caught on the two identifiers for frozen section slides, made all kinds of changes, but when we checked our new questions the questions was gone.? We have not gone back to our old ways but just an FYI. Susan T. Paturzo HT (ASCP) TJUH From Timothy.Morken <@t> ucsfmedctr.org Thu Jan 9 10:29:21 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Jan 9 10:29:31 2014 Subject: [Histonet] RE: slide labeling In-Reply-To: <9C8F910F72893643B3C3793C3D67132B013FF0AB@PATHOLOGYSERVER.pathologyarts.local> References: <9C8F910F72893643B3C3793C3D67132B013FEE89@PATHOLOGYSERVER.pathologyarts.local> <9C8F910F72893643B3C3793C3D67132B013FF0AB@PATHOLOGYSERVER.pathologyarts.local> Message-ID: <761E2B5697F795489C8710BCC72141FF36736735@ex08.net.ucsf.edu> "" The error here occurred when writing the number on the slide,..."" >From experience I know that the hand-written label is the glaring weak link. There are so many ways to do that wrong! Mis-numbering happens all the time by transposing numbers, leaving a number off, being illegible, and just memory issues - I've seen slides that were labeled with the previous case number because the person labeling simply had that number stuck in their head. When a tech is writing hundreds of slides per day there is no way to prevent these kinds of mistakes. Expand that over a many techs every day...we catch them daily at the block/slide matching station. IF you enforce one block to one slide labeling using barcode scanning of the block and on-demand printing of the slide AT THE MICROTIOME, then the labeling errors should be nearly eliminated. One example I saw from a real life lab went from 1000 mis-labels per year to less than 10. And each of those "mistakes" were deliberate shortcuts of the system. So when the system was followed, there were no mis-labeled slides. We plan to forgo hand writing on slides and just apply a label at the microtome from a scanned block. That makes some nervous, but the key is to make everyone understand, and enforce one-block on-demand label (or direct slide) printing. As a check we will still compare blocks to slides after staining. These "engineered controls" are usually the best way to eliminate mistakes because they force a person to do it the right way. I think the key to enforcing that is to have everyone understand that if they follow the method they will not make mistakes. And I tell our techs that few pathologists will remember (or even know) how fast they are at cutting - they will, however, remember every mistake that gets though! As to the CAP, the CAP does not say anything about how your institution should label a slide, except to recommend (and sometimes require, in the case of (pre-accessioned samples) two separate identifiers. The labeling method should be in your SOP's and discrepancies documented and resolved from QA investigations. Whether one or each number is "recognized" is up to you. In practice that is not really a solution because, as you show, either number could be the wrong number, so "designating" one of the numbers as the "correct" number will lead to mistakes at some point. The solution is simply to resolve those cases that end up with two different numbers and document/resolve why that happened. But, as I outline above, using hand-written labels is always going to lead to a certain level of mistakes. So maybe you just live with that if the other institution is requiring that to be done. CAP is working on a universal slide labeling proposal. We'll see what gems they come up with! Tim Morken Supervisor,Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Sent: Tuesday, January 07, 2014 11:28 AM To: Curt; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: slide labeling To add clarity, there is a 2D barcode on the block, we scan at microtomy and then label the slide. The system is really idiot proof "ASSUMING" the techs cut and label one block at a time, which we strictly enforce. The error here occurred when writing the number on the slide, I think he looked at the wrong block here, so in effect he did label a number of slides at the same time but the slide label was already affixed to the slide. I guess the rule should be to write the number as it's seen on the slide. Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Curt Sent: Tuesday, January 07, 2014 10:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide labeling So we have a facility we do some work for that requires both the hand written number and the slide label be viewable, so we place the slide label a little lower and write the number on the top of the frosted end. Does CAP recognize both numbers as a legal record? The reason I ask is because there was a case that the hand written number was wrong but the slide label was correct. That being said, there's still a QA issue with the slide not being accurately labeled. Slides are labeled at microtomy too, the hand written number and the slide label. I contend internally that the slide WAS accurately labeled because the sticker number and block number correspond accurately, the discrepancy was in the hand written number. Thoughts? Does CAP have any guidance on this? Thanks, Curt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttroyer <@t> petersonlab.com Thu Jan 9 11:00:40 2014 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Thu Jan 9 11:00:47 2014 Subject: [Histonet] Logging in specimens Message-ID: <6F13544BA3FB40778CB86716B5414AB1@Peterson.local> How do other facilities determine which physician receives a copy of the final pathology report. Is it indicated on the requisition and if so what information are you requesting so the correct physician receives the report. Thanks Travis From jsjurczak <@t> comcast.net Thu Jan 9 11:10:07 2014 From: jsjurczak <@t> comcast.net (jsjurczak@comcast.net) Date: Thu Jan 9 11:10:35 2014 Subject: Fw: [Histonet] The Biopsy Chip for high throughput pathology in prostate cancer (and other organs, the list is open..) In-Reply-To: <1389212817.93005.YahooMailNeo@web161703.mail.bf1.yahoo.com> Message-ID: <378390708.14474539.1389287407863.JavaMail.root@comcast.net> On Wednesday, January 8, 2014 10:19 PM, Sorin Musat wrote: Actually, it was easier than expected to convince the urologists to place the biopsies in the grooves of the chip (it did not take longer that the "traditional method" - I timed the first few patients and than it was an easy sell). Particularly since they were provided with a 2-D map of the prostate for them to mark the location of the individual biopsies. Of course there is a system of labeling the rows. The first one is dyed in a different color and/or there is a notch at the top left corner. Some people prefer chips with only 6 grooves (so they use one chip for the left lobe, the other one for the right lobe). Others even employ chips for 12 biopsies (one chip=one patient). The nice thing is that the biopsies keep the right orientation with regards to the entry point of the needle and one can easily draw some sort of 3-D representation of the cancer. We do not have here whole slide scanners and the software to do 3D imaging/reconstruction but it should be very easy. Also, even when the biopsy is coming out fragmented, the individual pieces of the biopsy will stay in place and not migrate. If you want, I can send you a presentation with real pictures and some stats on the first 1000 patients who benefited of the Biopsy Chip. A photograph is worth a thousand words. Sorin On Wednesday, January 8, 2014 9:29 PM, Curt wrote: Interesting, are you having much success convincing the clinician to take the time to us the device? Looks like they might be required to be extremely careful as opposed to just shooting a core into a specimen jar. Also, how about specimen identy/location, are the rows numbered or something along those lines. Something unique about each row to differentiate one from the other? Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sorin Musat Sent: Tuesday, January 07, 2014 10:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] The Biopsy Chip for high throughput pathology in prostate cancer (and other organs, the list is open..) Dear Histoneters, I am a new subscriber to Histonet, albeit I've been following this great website for at least 10 years. I would like to post some information about a new medical device I invented and patented (the Biopsy Chip). This device was intended for increasing the efficiency of processing and sectioning prostate biopsies, but can be used for other organs that require multiple biopsies (breast, thyroid, skin, etc.,) as well as for friable tissues (bone marrow). For a quick preview, I uploaded on youtube a little movie clip we presented at The Romanian Annual Congress of Urology in 2011 (summarizing the results of our first pilot study): "High Throughput Biopsy Chip for the detection of Prostate Cancer": http://www.youtube.com/watch?v=zP1iiE3CEn0 After we patented and registered the medical device under the authority of the Romanian Ministry of Health, this technique was employed for over 2000 patients so far in a number of Romanian hospitals. We had very good results (significant economies in time and consumables + higher yield of tissue on the slides + more tissue left within the block + increased accuracy of the diagnostic). We already presented a couple of abstracts at 3 Urology conferences and we will publish very soon a full-length paper discussing our results. We believe that the Biopsy Chip is the first application of tissue microarrays in clinical diagnostic. Presently we are trying to find collaborators in order to test the Biopsy Chip for other organs/pathologies besides prostate cancer. We welcome any comments and suggestions. If anyone wants more information about this device I will be very happy to answer your questions. If appropriate, I could also upload a presentation on Histonet server. Sincerely Sorin Musat, MD, PhD THEMIS Pathology (formerly HistoBest Inc, Edmonton, AB, Canada) Bucharest, Romania mobile: (4)0768 735 194 / 0725 653 551 email: s_musat@yahoo.com skype: sorin.musat2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From toni <@t> vanguarddermatology.com Thu Jan 9 11:35:32 2014 From: toni <@t> vanguarddermatology.com (toni@vanguarddermatology.com) Date: Thu Jan 9 11:35:36 2014 Subject: [Histonet] Looking for VIP 2000-3000 Message-ID: <20140109103532.7e88f44be474d1487ceaf5c3dfa2c7c1.7bfce19d11.mailapi@email02.secureserver.net> I am looking for a VIP 2000 or 3000; preferrably benchtop but a floor model will not be turned away. Does anyone know of a reliable source to start looking for this machine I see some on sale at random sites but not sure of the site integrity. So if there are any goods sites that are know please let me know ...Desperate and about to throw this Leica ASP300 off the roof. Thank you From doug.porter <@t> caplab.org Thu Jan 9 12:00:45 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Thu Jan 9 11:56:54 2014 Subject: [Histonet] Looking for VIP 2000-3000 In-Reply-To: <20140109103532.7e88f44be474d1487ceaf5c3dfa2c7c1.7bfce19d11.mailapi@email02.secureserver.net> References: <20140109103532.7e88f44be474d1487ceaf5c3dfa2c7c1.7bfce19d11.mailapi@email02.secureserver.net> Message-ID: <009101cf0d64$b8f701c0$2ae50540$@caplab.org> http://www.rankinbiomed.com/ Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of toni@vanguarddermatology.com Sent: Thursday, January 09, 2014 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for VIP 2000-3000 I am looking for a VIP 2000 or 3000; preferrably benchtop but a floor model will not be turned away. Does anyone know of a reliable source to start looking for this machine I see some on sale at random sites but not sure of the site integrity. So if there are any goods sites that are know please let me know ...Desperate and about to throw this Leica ASP300 off the roof. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Jan 9 11:57:38 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jan 9 11:57:42 2014 Subject: [Histonet] Logging in specimens In-Reply-To: <6F13544BA3FB40778CB86716B5414AB1@Peterson.local> References: <6F13544BA3FB40778CB86716B5414AB1@Peterson.local> Message-ID: required field in data entry, legally authorizing MD default, and then additional or consulting clients/MD's wanting report can get copies, need NPI #, full address for MD or provider. How they get it depends on what is set up for that client ( EMR, email, fax) and part of client intake, that part handled by client services. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: ttroyer@petersonlab.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 9 Jan 2014 11:00:40 -0600 > Subject: [Histonet] Logging in specimens > > How do other facilities determine which physician receives a copy of the final pathology report. Is it indicated on the requisition and if so what information are you requesting so the correct physician receives the report. > > Thanks > Travis > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Thu Jan 9 12:13:48 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Jan 9 12:14:04 2014 Subject: [Histonet] Compressor for a Thermofisher 620E Cryostat Message-ID: We are trying to locate a new compressor for a Thermofisher 620 E cryostat that is around 7-8 years old. We are working through a third party service department and am getting conflicting information on whether there are still parts for this instrument. I have asked my Thermfisher rep but not heard back from her yet. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From fredandlaura914 <@t> sbcglobal.net Thu Jan 9 13:51:24 2014 From: fredandlaura914 <@t> sbcglobal.net (fredandlaura914@sbcglobal.net) Date: Thu Jan 9 14:20:38 2014 Subject: [Histonet] Histology and hip replacement Message-ID: <23F15CFE-B18A-4243-ACA3-DB6809BCC3A5@sbcglobal.net> Happy New Year out there in Histoland, Here in Dayton Ohio, we're coming out of the deep freezer back into the refrigerator. I just had hip replacement surgery. Beats some ball dropping on Times Square, right? I'm a one person lab, and am looking for some input on issues I'll be dealing with when I get back to work. Ergonomics, etc. Thanks in advance, Fred Sent from my iPad From Timothy.Morken <@t> ucsfmedctr.org Thu Jan 9 17:42:49 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Jan 9 17:43:05 2014 Subject: [Histonet] slide labeling In-Reply-To: References: <9C8F910F72893643B3C3793C3D67132B013FEE89@PATHOLOGYSERVER.pathologyarts.local> Message-ID: <761E2B5697F795489C8710BCC72141FF36740B81@ex07.net.ucsf.edu> 'BTW - CAP & NSH are working on a standard labeling guidelines for AP. I" I read this over (search "Uniform Labeling of slides and blocks" and the pdf from the NSH site will appear). What is notable is the number of comments from labs that are still hand-writing all their blocks and slides. The primary comment is that there is no room to write all that CAP suggests. Even some with older slide labelers commented that they could not fit the information on the cassette face. Modern labelers eliminate that problem with much better resolution and formatting of the information. We print a 2D code, Accession number and patient name on all blocks. There is plenty of room due to variable font sizes for various elements. Of course, it takes money to get newer equipment so it will continue to be a problem for some labs. Another discussion throughout is what constitutes a "unique" identifier. Many comment that a name is not unique and can be truncated. (however, a combination of number and name will be unique, though having several numbers with the same last name, but different patients, can be confusing. Hopefully at least part of the first name can be included). Having a first name or initial as well will greatly add to the probability of a name and number being unique. Some contended that a number alone was ok because I can be traced back to two identifiers. Unless the number is wrong.... Others thought that a barcode should be accepted as a second identifier. I don't' think it would work - the code usually has the identical information as the accession number so is only redundant and does not add to the uniqueness of the information. A second identifier must have different information that adds to the uniqueness. Also, you need a scanner to make use of it. Human-readable information is still going to be useful for troubleshooting. One interesting thought is that if blocks and slide labels are being printed directly from an LIS (no barcode), are two identifiers necessary to positively ID the item? Probably not. However, being human, a person could mis-read a number, and then a second identifier can confirm the ID or not. But what if you are printing barcodes on blocks and slides and then scanning barcodes (or 2D codes) to bring up a case in the LIS? Then technically a second identifier is not necessary. There could still be exceptions - if the barcode cannot be read, then the user would need to type in the info, and then a mistake could be made, and therefore a second ID is useful to confirm it. Overall it seems a second identifier greatly both increases the probability of uniqueness and increases the probability of catching typo mistakes when info is hand-written or hand-entered via keyboard. Personally I like the number/name combination because it is too easy to confuse things with two different numbers . Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, January 09, 2014 6:27 AM To: Curt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] slide labeling CAP has requirement for 2 identifiers on slides for intra-operative, such as frozen sections only as far I know. That's for slides since the case may not yet have an accession #. The blocks, 2 identifiers. For specimen integrity though, ( CAP required all over the place) you really want two different, unique identifiers on everything that you track through the whole process. I like your idea with the labeling, but the intent is to have two identifiers and to cross check them. I know how harsh the response can be sometimes when a "human" error ( such as failing to catch the discrepancy ) is made with an entirely flawed, and "human dependant" system. There is a tendancy to cast blame. I don't like using patient names on work product much personally, but with at least a partial manual system ( I have that too) it becomes necessary. I just flip the slide over and read what is under the label, match the name & number with the log, or LIS, and match the block with the section on the slide. Still not perfect. For ease of follow up- I just define what constitutes an "error", and how many you are "permitted" ( yes I know, its really none), # before there are consequences. BTW - CAP & NSH are working on a standard labeling guidelines for AP. It is in the "works" and defines a lot of these issues and more, I found it helpful. Not sure when it will be published. A draft is probably on CAP and NSH sites. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: c.tague@Pathologyarts.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 7 Jan 2014 18:33:26 +0000 > Subject: [Histonet] slide labeling > > So we have a facility we do some work for that requires both the hand written number and the slide label be viewable, so we place the slide label a little lower and write the number on the top of the frosted end. Does CAP recognize both numbers as a legal record? The reason I ask is because there was a case that the hand written number was wrong but the slide label was correct. That being said, there's still a QA issue with the slide not being accurately labeled. Slides are labeled at microtomy too, the hand written number and the slide label. I contend internally that the slide WAS accurately labeled because the sticker number and block number correspond accurately, the discrepancy was in the hand written number. > > Thoughts? Does CAP have any guidance on this? > > Thanks, > > Curt > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Jan 9 18:22:59 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jan 9 18:23:04 2014 Subject: [Histonet] slide labeling In-Reply-To: <761E2B5697F795489C8710BCC72141FF36740B81@ex07.net.ucsf.edu> References: <9C8F910F72893643B3C3793C3D67132B013FEE89@PATHOLOGYSERVER.pathologyarts.local>, , <761E2B5697F795489C8710BCC72141FF36740B81@ex07.net.ucsf.edu> Message-ID: Yes, I also contributed comments to the public review/survey and read through the comments of others as they were compiled, and found them interesting. Agree Tim, that is why I like two identifiers of some kind even when it is not required. I don't see the logic of doing this at some steps, but not others. I like it all the way through, beginning to end. There are problems with numbers, fatigue and people, agree. I still resist the name on the slide for space reasons mostly, and prefer one barcode or QR code and one "human readable". I add to that physical matching of the blocks with the slides. I also like to make cross checking steps formally part of the process for every slide, not just "suspicious" ones. Part being paranoid I suppose and partly because I have not been given any bar coding or other technology solution where I work now, and I got kind of used to having something. There is probably no perfection, but whatever you can think of and do in your particular system and situation for ID verification, has to be positive if it reduces errors and increase patient safety. It will be interesting to see the final version and how much impact it has on standardizing labeling practices....stay tuned I guess. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: joelleweaver@hotmail.com; c.tague@pathologyarts.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] slide labeling > Date: Thu, 9 Jan 2014 23:42:49 +0000 > > 'BTW - CAP & NSH are working on a standard labeling guidelines for AP. I" > > I read this over (search "Uniform Labeling of slides and blocks" and the pdf from the NSH site will appear). > > What is notable is the number of comments from labs that are still hand-writing all their blocks and slides. The primary comment is that there is no room to write all that CAP suggests. Even some with older slide labelers commented that they could not fit the information on the cassette face. Modern labelers eliminate that problem with much better resolution and formatting of the information. We print a 2D code, Accession number and patient name on all blocks. There is plenty of room due to variable font sizes for various elements. Of course, it takes money to get newer equipment so it will continue to be a problem for some labs. > > Another discussion throughout is what constitutes a "unique" identifier. Many comment that a name is not unique and can be truncated. (however, a combination of number and name will be unique, though having several numbers with the same last name, but different patients, can be confusing. Hopefully at least part of the first name can be included). Having a first name or initial as well will greatly add to the probability of a name and number being unique. > > Some contended that a number alone was ok because I can be traced back to two identifiers. Unless the number is wrong.... > > Others thought that a barcode should be accepted as a second identifier. I don't' think it would work - the code usually has the identical information as the accession number so is only redundant and does not add to the uniqueness of the information. A second identifier must have different information that adds to the uniqueness. Also, you need a scanner to make use of it. Human-readable information is still going to be useful for troubleshooting. > > One interesting thought is that if blocks and slide labels are being printed directly from an LIS (no barcode), are two identifiers necessary to positively ID the item? Probably not. However, being human, a person could mis-read a number, and then a second identifier can confirm the ID or not. > > But what if you are printing barcodes on blocks and slides and then scanning barcodes (or 2D codes) to bring up a case in the LIS? Then technically a second identifier is not necessary. There could still be exceptions - if the barcode cannot be read, then the user would need to type in the info, and then a mistake could be made, and therefore a second ID is useful to confirm it. > > Overall it seems a second identifier greatly both increases the probability of uniqueness and increases the probability of catching typo mistakes when info is hand-written or hand-entered via keyboard. > > Personally I like the number/name combination because it is too easy to confuse things with two different numbers . > > > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, January 09, 2014 6:27 AM > To: Curt; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] slide labeling > > CAP has requirement for 2 identifiers on slides for intra-operative, such as frozen sections only as far I know. That's for slides since the case may not yet have an accession #. The blocks, 2 identifiers. > > For specimen integrity though, ( CAP required all over the place) you really want two different, unique identifiers on everything that you track through the whole process. I like your idea with the labeling, but the intent is to have two identifiers and to cross check them. I know how harsh the response can be sometimes when a "human" error ( such as failing to catch the discrepancy ) is made with an entirely flawed, and "human dependant" system. There is a tendancy to cast blame. > I don't like using patient names on work product much personally, but with at least a partial manual system ( I have that too) it becomes necessary. I just flip the slide over and read what is under the label, match the name & number with the log, or LIS, and match the block with the section on the slide. Still not perfect. For ease of follow up- I just define what constitutes an "error", and how many you are "permitted" ( yes I know, its really none), # before there are consequences. > > BTW - CAP & NSH are working on a standard labeling guidelines for AP. It is in the "works" and defines a lot of these issues and more, I found it helpful. Not sure when it will be published. A draft is probably on CAP and NSH sites. > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: c.tague@Pathologyarts.com > > To: histonet@lists.utsouthwestern.edu > > Date: Tue, 7 Jan 2014 18:33:26 +0000 > > Subject: [Histonet] slide labeling > > > > So we have a facility we do some work for that requires both the hand written number and the slide label be viewable, so we place the slide label a little lower and write the number on the top of the frosted end. Does CAP recognize both numbers as a legal record? The reason I ask is because there was a case that the hand written number was wrong but the slide label was correct. That being said, there's still a QA issue with the slide not being accurately labeled. Slides are labeled at microtomy too, the hand written number and the slide label. I contend internally that the slide WAS accurately labeled because the sticker number and block number correspond accurately, the discrepancy was in the hand written number. > > > > Thoughts? Does CAP have any guidance on this? > > > > Thanks, > > > > Curt > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Thu Jan 9 18:31:54 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jan 9 18:31:58 2014 Subject: [Histonet] Histology and hip replacement In-Reply-To: <23F15CFE-B18A-4243-ACA3-DB6809BCC3A5@sbcglobal.net> References: <23F15CFE-B18A-4243-ACA3-DB6809BCC3A5@sbcglobal.net> Message-ID: Fred Don't know specifics about your medical limitations, however the current issue of Micro-Graf, the newsletter publication from the Michigan society, has a great piece about ergonomics and that goes specifically into histology tasks and ergonomic concerns. I am pretty sure this article would be posted on the web site if you don't get the mailing, or you might be able to contact them via the contacts on the web site for a copy. Check it out and see if this article might be helpful to you. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: fredandlaura914@sbcglobal.net > Date: Thu, 9 Jan 2014 14:51:24 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology and hip replacement > > Happy New Year out there in Histoland, > > Here in Dayton Ohio, we're coming out of the deep freezer back into the refrigerator. I just had hip replacement surgery. Beats some ball dropping on Times Square, right? I'm a one person lab, and am looking for some input on issues I'll be dealing with when I get back to work. Ergonomics, etc. > > Thanks in advance, > Fred > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Thu Jan 9 18:46:48 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Jan 9 18:46:53 2014 Subject: [Histonet] TAT for Transplant Biopsies Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing? Do any of you handle specimens at night, weekends, or holidays? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Tony.Reilly <@t> health.qld.gov.au Thu Jan 9 20:24:44 2014 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Thu Jan 9 20:25:09 2014 Subject: [Histonet] RE: TAT for Transplant Biopsies In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> Message-ID: Hi Richard This will depend on the working hours of your lab. The hospital I work in now performs renal and liver transplants. Clinicians know that they must have their biopsies in the lab by 1pm for a same day result including all of the special stains. In this lab the lab staff work until 6pm but most pathologists are heading home between 5-5.30pm. At a previous position the hospital performed heart and lung transplants. That lab finished at 5.30pm for both lab and pathologist staff. The cut off for same day result was 2pm. The heart biopsies only required H&Es and while the lung biopsies needed an Orcein/H&E and a Grocott. We had developed a rapid Grocott stain (20 minutes) which was completed before the H&E run on the stainer had finished. All slides were with the pathologist before 5pm. Genuine urgent cases were done out of hours but the clinician had to speak with the reporting pathologist to determine the degree of urgency. Some renals will be totally completed if required and some will be a H&E result only. Regards Tony Tony Reilly? B.App.Sc. ,?M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency |?Department of?Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA? Qld 4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony.reilly@health.qld.gov.au Web:? www.health.qld.gov.au/qhcss/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, 10 January 2014 10:47 AM To: Tony Reilly; Histonet Subject: [Histonet] TAT for Transplant Biopsies For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing? Do any of you handle specimens at night, weekends, or holidays? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From tony.henwood <@t> health.nsw.gov.au Thu Jan 9 23:24:41 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jan 9 23:24:57 2014 Subject: [Histonet] RE: TAT for Transplant Biopsies In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> Message-ID: <6D6BD1DE8A5571489398B392A38A7157D61B0B92@xmdb01.nch.kids> Hi Richard, Unfortunately we do not have a cut-off time for urgent transplant biopsies (its seems a kid's hospital is different). We microwave process kidney, lung and liver biopsies, about 30 minutes in total, though if we can get away with it we extend this by 30 minutes for more fixation, using 10%NBF and microwave. We use a Micromed TT. We can then do this out of hours and weekends - cost a bit in overtime though, but hopefully it is worth it. The morphology is nearly always better than routine processing (using ethanol and xylene) and my pathologists prefer this for routine renal biopsies as well. There seems to be less leaching out of cytoplasmic substances compared to the routinely processed biopsies. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, 10 January 2014 11:47 AM To: Histonet Subject: [Histonet] TAT for Transplant Biopsies For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing? Do any of you handle specimens at night, weekends, or holidays? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From leila.etemadi <@t> med.lu.se Fri Jan 10 07:24:05 2014 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Fri Jan 10 07:24:29 2014 Subject: [Histonet] melanin pigments Message-ID: <4019AD42-A0A3-44F6-BB61-88579692E5DB@med.lu.se> Hello every body, and Happy new year. First I would like to thank you all, for your professional guidances and valuable advices during last year. They were such great improvement in my work as a beginner in this fantastic field. Thank you! Then I wonder if any one has experience for extracting melanin pigments from animal eyes?, Any guidance is highly appreciated. I wish you all the best in new year, Leila :-) From Joyce.Weems <@t> emoryhealthcare.org Fri Jan 10 07:42:27 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jan 10 07:42:36 2014 Subject: [Histonet] RE: TAT for Transplant Biopsies In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> Message-ID: 11:30 for our hearts Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, January 09, 2014 7:47 PM To: Histonet Subject: [Histonet] TAT for Transplant Biopsies For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing? Do any of you handle specimens at night, weekends, or holidays? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Ronald.Houston <@t> nationwidechildrens.org Fri Jan 10 08:23:51 2014 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jan 10 08:23:57 2014 Subject: [Histonet] RE: TAT for Transplant Biopsies In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> Message-ID: Richard, being a peds hospital, we are somewhat similar to Tony in Australia. If the clinician needs the results that day, that's what he gets (they don't abuse this either!). If on a Friday (or preceding a holiday), and they do not need the result that same day, we will process overnight and someone will come in and cut and stain the bx the following day. To date, we have not had to come in on a Sunday, but someone is on-call for this (and other emergencies). We do not use microwaves, but have great success using the Leica Peloris on a 1 hour cycle, after a minimum of 30 minutes fixation. We will not rush the special stains or IHC (C3d and C4d), but our regular cut-off time for those are 3:00pm for same day TAT and 4:30 for overnight IHC Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, January 09, 2014 7:47 PM To: Histonet Subject: [Histonet] TAT for Transplant Biopsies For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing? Do any of you handle specimens at night, weekends, or holidays? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Jan 10 09:47:41 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Jan 10 09:47:55 2014 Subject: [Histonet] RE: TAT for Transplant Biopsies In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> Message-ID: <761E2B5697F795489C8710BCC72141FF36740C51@ex07.net.ucsf.edu> Hi Rich, We accept transplant bx until 10am for same day results H&E's out by 2pm. We offer same day on Saturdays and two days of a 4-day holiday weekends. We can do EM same day as well if necessary. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, January 09, 2014 4:47 PM To: Histonet Subject: [Histonet] TAT for Transplant Biopsies For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing? Do any of you handle specimens at night, weekends, or holidays? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Fri Jan 10 10:30:52 2014 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Jan 10 10:30:56 2014 Subject: [Histonet] Melanin pigment removal Message-ID: <8D0DC387658DCC4-2098-471AF@webmail-m222.sysops.aol.com> Leila asks about removing melanin pigment. We run slides down to water and place in aquous 0.25% potassium Permanganate. Wash well in tap water.Rinse in distilled water. Place in aquous 5% oxalic acid for 5 minutes. Rinse in distilled water.Wash in tap water. continue with method. We run three control slides. Two of a known melanin containing slide, and an extra test slide. Treat one each of the test slides and control slides with the permanganate. The method is a little harsh. You may want to use coated slides. There are different melanins that have slightly different characteristics, requiring different times. Ref: Manual of Histological Staining Methodsof the Armed Forces Institute of Pathology (1968) Lee Luna (Edit) There are other methods to remove melanin that use potassium chlorate, and hydrogen peroxide. Michael Titford Pathology USA Mobile AL From mtitford <@t> aol.com Fri Jan 10 10:40:02 2014 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Jan 10 10:40:06 2014 Subject: [Histonet] Melanin removal p.s. Message-ID: <8D0DC39BDB69664-2098-47289@webmail-m222.sysops.aol.com> Thats's 5 minutes in the potassium permanaganate. Some stubborn melanin may need a longer. Some authors recommend as long as 24 hours. (It's Friday - how can you tell?) Michael Titford Pathology USA Mobila AL From fredandlaura914 <@t> sbcglobal.net Fri Jan 10 10:52:55 2014 From: fredandlaura914 <@t> sbcglobal.net (fredandlaura914@sbcglobal.net) Date: Fri Jan 10 10:53:01 2014 Subject: [Histonet] Histology and hip replacement In-Reply-To: <16F356143B1CE2459BC129BF68AD0F0F14EC8F15@PHSX10MB25.partners.org> References: <23F15CFE-B18A-4243-ACA3-DB6809BCC3A5@sbcglobal.net> <16F356143B1CE2459BC129BF68AD0F0F14EC8F15@PHSX10MB25.partners.org> Message-ID: Thanks Peggy, I've heard many positive accounts and they help me maintain a positive attitude. My main restrictions, for a few months are; no crossing of legs, no turning of feet or knees inward, and no bending at the hip past 90 degrees. Also, items need to be located between my legs before lifting. One of my concerns is the ability to move between microtome and water bath, while sitting, without violating those restrictions. I may need to get creative in the layout of the lab. On a positive note, the Leica RM2255 we purchased earlier in the year, helped alleviate my repetitive motion problems. Thank you Histonetters for helping me with that selection. I apologize for being so long winded. I guess spending the days locked inside, interacting only with a couple snoring dachshunds, might be taking it's toll. Fred Sent from my iPad > On Jan 9, 2014, at 3:36 PM, "Sherwood, Margaret" wrote: > > I've had 2 hip replacements; facing having the cup replaced on the first one (20 years since replaced). I have had no issues, ergonomically or otherwise. It made a world of difference in the quality of my life. > > Good luck! > > > > > Peggy Sherwood > Research Specialist, Photopathology > Wellman Center for Photomedicine (EDR 214) > Massachusetts General Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839 (voice mail) > 617-726-6983 (lab) > 617-726-1206 (fax) > msherwood@partners.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of fredandlaura914@sbcglobal.net > Sent: Thursday, January 09, 2014 2:51 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology and hip replacement > > Happy New Year out there in Histoland, > > Here in Dayton Ohio, we're coming out of the deep freezer back into the refrigerator. I just had hip replacement surgery. Beats some ball dropping on Times Square, right? I'm a one person lab, and am looking for some input on issues I'll be dealing with when I get back to work. Ergonomics, etc. > > Thanks in advance, > Fred > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > From Joyce.Weems <@t> emoryhealthcare.org Fri Jan 10 10:56:49 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jan 10 10:57:09 2014 Subject: [Histonet] Histology and hip replacement In-Reply-To: References: <23F15CFE-B18A-4243-ACA3-DB6809BCC3A5@sbcglobal.net> <16F356143B1CE2459BC129BF68AD0F0F14EC8F15@PHSX10MB25.partners.org> Message-ID: I would suggest that if you have a table (or can open a drawer and put a tray or board on it), put your water bath beside you. Then you wouldn't need to "push off". Maybe that would be a solution? Best wishes for a speedy and complete recovery! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of fredandlaura914@sbcglobal.net Sent: Friday, January 10, 2014 11:53 AM To: Sherwood, Margaret Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histology and hip replacement Thanks Peggy, I've heard many positive accounts and they help me maintain a positive attitude. My main restrictions, for a few months are; no crossing of legs, no turning of feet or knees inward, and no bending at the hip past 90 degrees. Also, items need to be located between my legs before lifting. One of my concerns is the ability to move between microtome and water bath, while sitting, without violating those restrictions. I may need to get creative in the layout of the lab. On a positive note, the Leica RM2255 we purchased earlier in the year, helped alleviate my repetitive motion problems. Thank you Histonetters for helping me with that selection. I apologize for being so long winded. I guess spending the days locked inside, interacting only with a couple snoring dachshunds, might be taking it's toll. Fred Sent from my iPad > On Jan 9, 2014, at 3:36 PM, "Sherwood, Margaret" wrote: > > I've had 2 hip replacements; facing having the cup replaced on the first one (20 years since replaced). I have had no issues, ergonomically or otherwise. It made a world of difference in the quality of my life. > > Good luck! > > > > > Peggy Sherwood > Research Specialist, Photopathology > Wellman Center for Photomedicine (EDR 214) Massachusetts General > Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839 (voice mail) > 617-726-6983 (lab) > 617-726-1206 (fax) > msherwood@partners.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > fredandlaura914@sbcglobal.net > Sent: Thursday, January 09, 2014 2:51 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology and hip replacement > > Happy New Year out there in Histoland, > > Here in Dayton Ohio, we're coming out of the deep freezer back into the refrigerator. I just had hip replacement surgery. Beats some ball dropping on Times Square, right? I'm a one person lab, and am looking for some input on issues I'll be dealing with when I get back to work. Ergonomics, etc. > > Thanks in advance, > Fred > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom > it is addressed. If you believe this e-mail was sent to you in error > and the e-mail contains patient information, please contact the > Partners Compliance HelpLine at http://www.partners.org/complianceline > . If the e-mail was sent to you in error but does not contain patient > information, please contact the sender and properly dispose of the e-mail. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From lblazek <@t> digestivespecialists.com Fri Jan 10 11:11:49 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Jan 10 11:11:55 2014 Subject: [Histonet] Histology and hip replacement In-Reply-To: References: <23F15CFE-B18A-4243-ACA3-DB6809BCC3A5@sbcglobal.net> <16F356143B1CE2459BC129BF68AD0F0F14EC8F15@PHSX10MB25.partners.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E39167BF40502@IBMB7Exchange.digestivespecialists.com> That's what I was going to suggest. "open a drawer and put a tray or board on it" We do that any way as a place to set the slides and other paraphernalia that you may need. It keeps from having to reach over or around. Linda Linda Blazek HT (ASCP) GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Friday, January 10, 2014 11:57 AM To: 'fredandlaura914@sbcglobal.net'; Sherwood, Margaret Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology and hip replacement I would suggest that if you have a table (or can open a drawer and put a tray or board on it), put your water bath beside you. Then you wouldn't need to "push off". Maybe that would be a solution? Best wishes for a speedy and complete recovery! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of fredandlaura914@sbcglobal.net Sent: Friday, January 10, 2014 11:53 AM To: Sherwood, Margaret Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histology and hip replacement Thanks Peggy, I've heard many positive accounts and they help me maintain a positive attitude. My main restrictions, for a few months are; no crossing of legs, no turning of feet or knees inward, and no bending at the hip past 90 degrees. Also, items need to be located between my legs before lifting. One of my concerns is the ability to move between microtome and water bath, while sitting, without violating those restrictions. I may need to get creative in the layout of the lab. On a positive note, the Leica RM2255 we purchased earlier in the year, helped alleviate my repetitive motion problems. Thank you Histonetters for helping me with that selection. I apologize for being so long winded. I guess spending the days locked inside, interacting only with a couple snoring dachshunds, might be taking it's toll. Fred Sent from my iPad > On Jan 9, 2014, at 3:36 PM, "Sherwood, Margaret" wrote: > > I've had 2 hip replacements; facing having the cup replaced on the first one (20 years since replaced). I have had no issues, ergonomically or otherwise. It made a world of difference in the quality of my life. > > Good luck! > > > > > Peggy Sherwood > Research Specialist, Photopathology > Wellman Center for Photomedicine (EDR 214) Massachusetts General > Hospital > 50 Blossom Street > Boston, MA 02114-2696 > 617-724-4839 (voice mail) > 617-726-6983 (lab) > 617-726-1206 (fax) > msherwood@partners.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > fredandlaura914@sbcglobal.net > Sent: Thursday, January 09, 2014 2:51 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology and hip replacement > > Happy New Year out there in Histoland, > > Here in Dayton Ohio, we're coming out of the deep freezer back into the refrigerator. I just had hip replacement surgery. Beats some ball dropping on Times Square, right? I'm a one person lab, and am looking for some input on issues I'll be dealing with when I get back to work. Ergonomics, etc. > > Thanks in advance, > Fred > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The information in this e-mail is intended only for the person to whom > it is addressed. If you believe this e-mail was sent to you in error > and the e-mail contains patient information, please contact the > Partners Compliance HelpLine at http://www.partners.org/complianceline > . If the e-mail was sent to you in error but does not contain patient > information, please contact the sender and properly dispose of the e-mail. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Fri Jan 10 11:58:15 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Jan 10 12:01:28 2014 Subject: [Histonet] controls Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E787@HPEMX3.HealthPartners.int> Does anyone out in histoland have a Candid control for fungus that you could spare? We are very much in need and would appreciate the help and see what we could possibly trade for. Much thanks and have a great week-end all! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From thisisann <@t> aol.com Fri Jan 10 15:46:39 2014 From: thisisann <@t> aol.com (Ann Specian) Date: Fri Jan 10 15:46:42 2014 Subject: [Histonet] Radiation SOP for Histology Message-ID: <8D0DC6493F2A0E9-170C-47C78@webmail-d175.sysops.aol.com> I need a radiation sop specific to Histology. Does anyone have one they can share? thanks, Ann From rsrichmond <@t> gmail.com Sat Jan 11 12:44:24 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jan 11 12:44:28 2014 Subject: [Histonet] Re: SOP for histology Message-ID: Ann Specian asks: "I need a radiation sop specific to Histology. Does anyone have one they can share?" Not a lot to say. It probably should make clear that there's no problem with technetium 99m in breast and sentinel node specimens. Often forgotten are the radioactive "seeds" that occasionally appear in prostate specimens - you need a little help from the radiology department with these. Bob Richmond Samurai Pathologist Maryville TN From koellingr <@t> comcast.net Sat Jan 11 14:03:13 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Jan 11 14:03:41 2014 Subject: [Histonet] OT-Retirement In-Reply-To: <2083395201.8295006.1389364262937.JavaMail.root@comcast.net> Message-ID: <1773927268.8989321.1389470593285.JavaMail.root@comcast.net> Hello all out there, ? This is regarding: Ray Koelling; currently from just north of the Seattle, WA area.? If you and I have connected in some way over the last 47 years,?the following?is a message?concerning my (semi)-retirement that?I hope you will enjoy and anyone with little or no interest?in?such writing or that knows me not, can?easily use the delete button right now to save themselves a few minutes of OT reading. ? It is time in life to shift a bit of focus after this long and amazing anatomic pathology journey.??There was?the?high school summer job in the mid 60's at a St. Louis histopath lab changing a Lipshaw linear, open air?tissue processor (we used dioxane both miscible with aqueous solutions?and paraffin and NOT the dioxin of Agent Orange infamy) along with?folding A LOT of paper boats for embedding and making 10% formalin from 55 gallon drums of 37% formaldehyde solution.? Somehow my brain has survived relatively unscathed.? Some may dispute that last sentence.? Working at Jackson Memorial Hospital in South Florida with the?great Dr. Azorides Morales and Dr. Mehred Nadji (actually Nadji was a resident when I was there and I have a?treasured picture of him kicked back at a?BBQ at my house wearing his famous sandals).? To learning more?advanced immunology, histology?and a lot more of cellular/molecular techniques?along with?the ability to critically think?during the 5 years (of both unbelievably positive heaven and unrelenting, unforgiving hell)?of graduate school.? To the biotechnology?world and working on such drugs as?etanercept, panitumumab, denosumab?and (still in testing) TSLP, a compound?that I had actually worked on in graduate school but when it was the newly discovered murine form TSLP and then also 50 other discovery molecules that all saw their shelving at various stages of development as failed candidates to progress in a particular pathway.? To then being able to work with Dr. Allen Gown in his?fantastic lab in the Seattle area.? So if we have run into one another, in person, on-line, at a meeting or anywhere in the last nearly half century, hope you are doing well and are keeping safe and I wish you the best. ? A?part of my time now is going to be spent on some K-12 education projects.? Organizing and helping at 2 different school districts for district-wide science fairs and STEM (science, technology, engineering, math) career festivals.? I am helping at the annual biotechnology fair in the Puget Sound Region for 300-400 high schoolers.? And am on Board of the Washington State Science and Engineering Fair that is the entrance point for this state into the big International Intel Science Fair.? Why?? It is no new, great news flash at all that?the US?is sinking further behind many countries?of the world in math and science education.? And that is to the severe, possibly life-long detriment of kids now who will be less able to compete, as adults,?with a global economy, jobs?and society of the 21st century and which?will?frankly revolve a lot around STEM issues whether you like it or not.? The world is simply not now, nor will ever be again, as it was with me holding a 4-year degree in biology/chemistry in 1973 and at that time having virtually unlimited access to whatever I wanted to do. ? Then for those kids K-12 who don't like math and science at all?and don't want to be in STEM or any kind of STEM career I have offered up this message to them.??Not liking STEM as a career?is perfectly fine.? You need to do in your life what your talents and dreams allow you to do.? Yet remember this.? No matter what non-STEM thing you do in life, you as a voting, tax-paying, living, breathing adult will be surrounded by STEM issues all throughout life.? You will be voting for/against issues or policies and for/against politicians and some, even many?of those issues are?STEM issues.? Radioactive storage waste in salt domes in Nevada?? Fracking in the upper?Midwest?? Coal vs. nuclear vs. "green energy" anywhere?? 5 cent plastic bags to cure global warming?? Healthcare?? Genetically modified foods?? Embryonic vs. other stem cell research?? Aging populations?? Disease???Mars yes or Mars no?? End-of-life issues?? Steroids and other drugs in the environment and food stuffs?? Sonar testing in oceans??100 other politically driven STEM-related issues.? How do adults now, and then you eventually when older, get your "science" information?? Journalists (on both sides of the political divide) see themselves as having a higher-level?moral obligation to now fine-tune and manipulate the news, including science news, instead of just reporting it.? Politicians (on both sides of the political divide) spew out any so-called "science" if it gets them more votes than they loose.? Talk show hosts (on both sides of the political divide) spew out "science" if it gets them more ratings than they loose.? Self-promoting, mainly amateurish-science?bloggers or tweeters (on both sides of the political divide) spew out "science" news to appease their own narcissistic proclivities. ? So no matter what you do in life, you will be in a world of STEM and do you want to think and act rationally and knowledgeably about such issues for the positive benefit of a human society or do you want to advocate for/against or vote for/against something related to STEM issues based upon purely emotional, knee-jerk, thoughtless ideology (from both sides of the political divide)?? You can see what a?horribly, messy?state of affairs?that later path has brought us to in this society.? So that is my message to the K-12 to use your brain, the most powerful computer ever invented, to THINK.? And why I'm??trying to do some of the things now and that?I think I'll have?fun with using?my career acquired ?knowledge with?and?in addition?hopefully do a little good along the way for the next generation.? No one will ever convince me that learning, or at least being exposed to, fundamental scientific principles and fundamental scientific thought processes, won't actually help anyone in almost all facets of their life and in decision making. ? Of course there?are also yard-chores, making dinner, golf, hurling, the accordion, re-learning my German, writing 2 books, genealogy research in Southern Germany and the Alsace-Lorraine region during a future trip, weight-training, Senior Olympics, doing the top of Mt. Fuji hike before too long, trip to New Zealand to see where the Hobbit/LOTR trilogy was made, etc. and even a few other various things I'm sure I'll find?to keep me busy. ? Best of luck to all, even if you deleted this?message?long ago to gain a bit of time.? I'll be still monitoring the HistoNet and maybe even throwing in my 2-cents about technical/scientific issues only,?if I think I have something useful and appropriate to share.? Keep in touch. ? Ray (Koelling) Lake Forest Park, WA ? ? From zerfasp <@t> ors.od.nih.gov Sat Jan 11 15:24:52 2014 From: zerfasp <@t> ors.od.nih.gov (Zerfas, Patricia (NIH/OD/ORS) [E]) Date: Sat Jan 11 15:26:37 2014 Subject: [Histonet] error rate for sectioning Message-ID: <997D36BD8D0ACF418AF900F4E781DE4414BCB4D6@MLBXv03.nih.gov> Hi All, What is the error rate set by you or your employer for paraffin sectioning? These are slides that are found to be unacceptable due to wrinkling, folding etc either by a pathologist or another reviewer. I was found to have 6 slides out of approx 1500 that I have sectioned to date to be unacceptable. I only perform this duty when I have completed all of my other work so this is not one of my regular duties. At times I have gone several months without sectioning and utilize a microtome that is approx. 26 years old. Since this was brought to my attention I have been given more up to date equipment which I have yet to utilize. Thanks in advance for your feedback. Patricia Zerfas From pruegg <@t> ihctech.net Sun Jan 12 13:29:27 2014 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sun Jan 12 13:29:32 2014 Subject: [Histonet] OT-Retirement In-Reply-To: <1773927268.8989321.1389470593285.JavaMail.root@comcast.net> Message-ID: <20140112122927.f86bd30e73b823f57b516b5451216a98.42d04ed4cf.mailapi@email01.secureserver.net> Very interesting post and life Ray, you should be proud of your accomplishments and contributions. I agree and and share your concerns about our inadequate STEM training for our up coming generations. Carl Sagan once said "our politicians and many inadequately trained teachers have a vested interest in not wanting the masses to think critically", because if they do they start asking questions the politicians and unfortunately many of the teachers feel uncomfortable answering because they most likely do not know the answers. It is too bad they feel threatened by questions they don't know the answers to rather than inspired to discovery the answers with the questioners. We all know how politicians and too many teachers want us to think they are "all knowing", it is the bases of their power over us. I believe this to be true and it really disturbs me. I to am now retired since I sold my lab business a year and half ago. I do try to do my part locally by serving on some boards at high schools promoting science education, especially laboratory science. Maybe if we work together we can make a difference in our future generations, I want them to crave the STEM knowledge just like I did. Let me know how I can help the cause to instill the passion for STEM knowledge so needed in our future generations. Best regards, Patsy Ruegg --------- Original Message --------- Subject: [Histonet] OT-Retirement From: koellingr@comcast.net Date: 1/11/14 1:03 pm To: histonet@lists.utsouthwestern.edu Hello all out there, This is regarding: Ray Koelling; currently from just north of the Seattle, WA area. If you and I have connected in some way over the last 47 years, the following is a message concerning my (semi)-retirement that I hope you will enjoy and anyone with little or no interest in such writing or that knows me not, can easily use the delete button right now to save themselves a few minutes of OT reading. It is time in life to shift a bit of focus after this long and amazing anatomic pathology journey. There was the high school summer job in the mid 60's at a St. Louis histopath lab changing a Lipshaw linear, open air tissue processor (we used dioxane both miscible with aqueous solutions and paraffin and NOT the dioxin of Agent Orange infamy) along with folding A LOT of paper boats for embedding and making 10% formalin from 55 gallon drums of 37% formaldehyde solution. Somehow my brain has survived relatively unscathed. Some may dispute that last sentence. Working at Jackson Memorial Hospital in South Florida with the great Dr. Azorides Morales and Dr. Mehred Nadji (actually Nadji was a resident when I was there and I have a treasured picture of him kicked back at a BBQ at my house wearing his famous sandals). To learning more advanced immunology, histology and a lot more of cellular/molecular techniques along with the ability to critically think during the 5 years (of both unbelievably positive heaven and unrelenting, unforgiving hell) of graduate school. To the biotechnology world and working on such drugs as etanercept, panitumumab, denosumab and (still in testing) TSLP, a compound that I had actually worked on in graduate school but when it was the newly discovered murine form TSLP and then also 50 other discovery molecules that all saw their shelving at various stages of development as failed candidates to progress in a particular pathway. To then being able to work with Dr. Allen Gown in his fantastic lab in the Seattle area. So if we have run into one another, in person, on-line, at a meeting or anywhere in the last nearly half century, hope you are doing well and are keeping safe and I wish you the best. A part of my time now is going to be spent on some K-12 education projects. Organizing and helping at 2 different school districts for district-wide science fairs and STEM (science, technology, engineering, math) career festivals. I am helping at the annual biotechnology fair in the Puget Sound Region for 300-400 high schoolers. And am on Board of the Washington State Science and Engineering Fair that is the entrance point for this state into the big International Intel Science Fair. Why? It is no new, great news flash at all that the US is sinking further behind many countries of the world in math and science education. And that is to the severe, possibly life-long detriment of kids now who will be less able to compete, as adults, with a global economy, jobs and society of the 21st century and which will frankly revolve a lot around STEM issues whether you like it or not. The world is simply not now, nor will ever be again, as it was with me holding a 4-year degree in biology/chemistry in 1973 and at that time having virtually unlimited access to whatever I wanted to do. Then for those kids K-12 who don't like math and science at all and don't want to be in STEM or any kind of STEM career I have offered up this message to them. Not liking STEM as a career is perfectly fine. You need to do in your life what your talents and dreams allow you to do. Yet remember this. No matter what non-STEM thing you do in life, you as a voting, tax-paying, living, breathing adult will be surrounded by STEM issues all throughout life. You will be voting for/against issues or policies and for/against politicians and some, even many of those issues are STEM issues. Radioactive storage waste in salt domes in Nevada? Fracking in the upper Midwest? Coal vs. nuclear vs. "green energy" anywhere? 5 cent plastic bags to cure global warming? Healthcare? Genetically modified foods? Embryonic vs. other stem cell research? Aging populations? Disease? Mars yes or Mars no? End-of-life issues? Steroids and other drugs in the environment and food stuffs? Sonar testing in oceans? 100 other politically driven STEM-related issues. How do adults now, and then you eventually when older, get your "science" information? Journalists (on both sides of the political divide) see themselves as having a higher-level moral obligation to now fine-tune and manipulate the news, including science news, instead of just reporting it. Politicians (on both sides of the political divide) spew out any so-called "science" if it gets them more votes than they loose. Talk show hosts (on both sides of the political divide) spew out "science" if it gets them more ratings than they loose. Self-promoting, mainly amateurish-science bloggers or tweeters (on both sides of the political divide) spew out "science" news to appease their own narcissistic proclivities. So no matter what you do in life, you will be in a world of STEM and do you want to think and act rationally and knowledgeably about such issues for the positive benefit of a human society or do you want to advocate for/against or vote for/against something related to STEM issues based upon purely emotional, knee-jerk, thoughtless ideology (from both sides of the political divide)? You can see what a horribly, messy state of affairs that later path has brought us to in this society. So that is my message to the K-12 to use your brain, the most powerful computer ever invented, to THINK. And why I'm trying to do some of the things now and that I think I'll have fun with using my career acquired knowledge with and in addition hopefully do a little good along the way for the next generation. No one will ever convince me that learning, or at least being exposed to, fundamental scientific principles and fundamental scientific thought processes, won't actually help anyone in almost all facets of their life and in decision making. Of course there are also yard-chores, making dinner, golf, hurling, the accordion, re-learning my German, writing 2 books, genealogy research in Southern Germany and the Alsace-Lorraine region during a future trip, weight-training, Senior Olympics, doing the top of Mt. Fuji hike before too long, trip to New Zealand to see where the Hobbit/LOTR trilogy was made, etc. and even a few other various things I'm sure I'll find to keep me busy. Best of luck to all, even if you deleted this message long ago to gain a bit of time. I'll be still monitoring the HistoNet and maybe even throwing in my 2-cents about technical/scientific issues only, if I think I have something useful and appropriate to share. Keep in touch. Ray (Koelling) Lake Forest Park, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Jan 13 08:36:45 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jan 13 08:36:54 2014 Subject: [Histonet] RE: TAT for Transplant Biopsies In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E018A4B05@HHCEXCHMB05.hhcsystem.org> Message-ID: <25A4DE08332B19499904459F00AAACB719DE6DEB9F@EVS1.archildrens.org> We accept biopsies until 10:30 a.m. Monday through Friday. If it is an extreme emergency, we will accept a later cut off time and someone stays to take care of it. Yes, we will process transplant biopsies on weekends and holidays. We do not process the biopsies at night. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, January 09, 2014 6:47 PM To: Histonet Subject: [Histonet] TAT for Transplant Biopsies For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing? Do any of you handle specimens at night, weekends, or holidays? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From LMurphy2 <@t> aultman.com Mon Jan 13 09:34:48 2014 From: LMurphy2 <@t> aultman.com (Leann M. Murphy) Date: Mon Jan 13 09:34:55 2014 Subject: [Histonet] Frozen sections Message-ID: Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio From Richard.Cartun <@t> hhchealth.org Mon Jan 13 09:46:32 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Jan 13 09:49:02 2014 Subject: [Histonet] ROS-1 In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E018A4F51@HHCEXCHMB05.hhcsystem.org> You might try contacting "Cell Signaling Technology" in Danvers, MA. They carry a rabbit mAb to ROS1 and I believe they have control slides that can be used for validation purposes. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Mark Tarango [marktarango@gmail.com] Sent: Friday, January 03, 2014 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ROS-1 Hi All, Does anyone have a case of lung tumor positive for ROS1 by either IHC or FISH? We are working on validating this by FISH and haven't seen a positive case. We are going to order a positive cell line but we'll be running this on tissue, so if anyone could spare a few slides or a block that would very helpful. thank you! Mark Tarango _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From jaylundgren <@t> gmail.com Mon Jan 13 10:39:25 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Jan 13 10:39:30 2014 Subject: [Histonet] controls In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E787@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4302ACF5B8E787@HPEMX3.HealthPartners.int> Message-ID: Is a candid control one that's taken when the fungus doesn't know you're sampling it? Sincerely? Jay A Lundgren, M.S., (HTL) ASCP On Fri, Jan 10, 2014 at 11:58 AM, Webb, Dorothy L < Dorothy.L.Webb@healthpartners.com> wrote: > Does anyone out in histoland have a Candid control for fungus that you > could spare? We are very much in need and would appreciate the help and > see what we could possibly trade for. > > Much thanks and have a great week-end all! > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Specialist > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this communication in error, please return it to the > sender immediately and delete the original message and any copy of it from > your computer system. If you have any questions concerning this message, > please contact the sender. Disclaimer R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From craigak12 <@t> gmail.com Mon Jan 13 10:46:25 2014 From: craigak12 <@t> gmail.com (Jb) Date: Mon Jan 13 10:46:34 2014 Subject: [Histonet] Processing: Message-ID: I have one tech telling me that when the entire processor is changed the tissue is too dry. We run a lot of fatty tissues, breast, etc on this processor. (Our biopsies are run on a separate processor). Is this correct, or should we only rotate reagents? No other techs complain. I have a hard time believing this, my experience is the opposite. Any input is appreciated. Sent from my iPhone From chapcl <@t> yahoo.com Mon Jan 13 10:54:58 2014 From: chapcl <@t> yahoo.com (Will Chappell) Date: Mon Jan 13 10:55:03 2014 Subject: [Histonet] Processing: In-Reply-To: References: Message-ID: <86500083-BCEC-4217-A9D0-71DCFDCD7B60@yahoo.com> This depends on so many different factors, however, I prefer a frequent rotation over a complete change. Do what is best for your tissue! Sent from my iPhone > On Jan 13, 2014, at 9:46 AM, Jb wrote: > > I have one tech telling me that when the entire processor is changed the tissue is too dry. We run a lot of fatty tissues, breast, etc on this processor. (Our biopsies are run on a separate processor). Is this correct, or should we only rotate reagents? No other techs complain. I have a hard time believing this, my experience is the opposite. Any input is appreciated. > > > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague <@t> Pathologyarts.com Mon Jan 13 13:07:17 2014 From: c.tague <@t> Pathologyarts.com (Curt) Date: Mon Jan 13 12:00:09 2014 Subject: [Histonet] Processing: In-Reply-To: References: Message-ID: <9C8F910F72893643B3C3793C3D67132B014025F3@PATHOLOGYSERVER.pathologyarts.local> This gets me back to another recent topic, soaking the blocks. I've seen this a little in the past, just soak them on an ice block,tray for a couple minutes and you'll be fine. To me, another indicator would be that if you're getting dry tissue when changed but not later could there be some kind of variation in results??? How often do you change the processors, all the tissue???? A complete change every other day would probably get you consistent results, at least, even if they are a little dry. Just my experience. Curt -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Monday, January 13, 2014 8:46 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing: I have one tech telling me that when the entire processor is changed the tissue is too dry. We run a lot of fatty tissues, breast, etc on this processor. (Our biopsies are run on a separate processor). Is this correct, or should we only rotate reagents? No other techs complain. I have a hard time believing this, my experience is the opposite. Any input is appreciated. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From turkekul <@t> gmail.com Mon Jan 13 12:06:40 2014 From: turkekul <@t> gmail.com (Mesru T) Date: Mon Jan 13 12:06:46 2014 Subject: [Histonet] myeloperoxidase on FFPE or frozen sections Message-ID: Dear Histonetters, I wonder if anyone has done myeloperoxidase stain on FFPE or frozen lung sections? I would appreciate any help. Regards, Mesru From pathrm35 <@t> verizon.net Mon Jan 13 12:14:41 2014 From: pathrm35 <@t> verizon.net (pathrm35@verizon.net) Date: Mon Jan 13 12:14:44 2014 Subject: [Histonet] per diem tech in Boston area Message-ID: <30499803.38451.1389636881602.JavaMail.root@vznit170164> Fellow techs, I have a per diem histo tech position in the Boston area. We are a small uropath lab and the duties will include embedding, sectioning and IHC's. thanks, Ron Martin From gu.lang <@t> gmx.at Mon Jan 13 12:56:12 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Jan 13 12:56:19 2014 Subject: [Histonet] microwave processing Message-ID: <000001cf1091$21344610$639cd230$@gmx.at> Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang From trathborne <@t> somerset-healthcare.com Mon Jan 13 12:58:44 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Jan 13 12:59:08 2014 Subject: [Histonet] RE: Frozen sections In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95AFB5B@SMCMAIL01.somerset-healthcare.com> Our pathologists prefer to perform all aspects of frozen section preparation. We will stain for them, but they would rather gross and section themselves. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Monday, January 13, 2014 10:35 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen sections Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Mon Jan 13 13:03:10 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Mon Jan 13 13:04:04 2014 Subject: [Histonet] microwave processing In-Reply-To: <000001cf1091$21344610$639cd230$@gmx.at> References: <000001cf1091$21344610$639cd230$@gmx.at> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF266@CUAEXH1.GCU-MD.local> Perhaps you can get some literature from one of the vendors that sell that technology. Milestone Medical Sakura Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.lang@gmx.at] Sent: Monday, January 13, 2014 1:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From tbraud <@t> holyredeemer.com Mon Jan 13 13:20:41 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Jan 13 13:20:49 2014 Subject: [Histonet] RE: Techs grossing FS tissue In-Reply-To: <20140113172526.46F481E8060@trendmess-svr.holyredeemer.local> References: <20140113172526.46F481E8060@trendmess-svr.holyredeemer.local> Message-ID: This is the only way that a non-pathologist can gross tissue, whether for frozen section or otherwise. They must meet CLIA standards for high complexity testing and furthermore, CAP says that the exact nature of the tissue grossed must be spelled out, along with nature of the pathologists' supervision (direct, indirect), and have at least annual competencies on grossing on file. That's the basic rules. State licensing may possibly add, but never subtract to this. However, there is nothing that says a tech can't plop a small singular piece of tissue onto a chuck to freeze and get things started. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Message: 3 Date: Mon, 13 Jan 2014 15:34:48 +0000 From: "Leann M. Murphy" Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Timothy.Morken <@t> ucsfmedctr.org Mon Jan 13 13:26:32 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Jan 13 13:26:42 2014 Subject: [Histonet] microwave processing In-Reply-To: <000001cf1091$21344610$639cd230$@gmx.at> References: <000001cf1091$21344610$639cd230$@gmx.at> Message-ID: <761E2B5697F795489C8710BCC72141FF367420A2@ex07.net.ucsf.edu> Gudrun, A good overview is here: http://www.ebsciences.com/papers/mw_tech.htm A couple old books: "The Microwave Cookbook for Microscopists", Boon and Kok Microwave Applications in Pathology [Hardcover] Anthony S. -Y Leong This person also wrote a lot of articles back in the 1980's and '90s on the subject Also a book by Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Monday, January 13, 2014 10:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jan 13 14:55:39 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 13 14:55:45 2014 Subject: [Histonet] microwave processing In-Reply-To: <000001cf1091$21344610$639cd230$@gmx.at> References: <000001cf1091$21344610$639cd230$@gmx.at> Message-ID: <1389646539.84582.YahooMailNeo@web120405.mail.ne1.yahoo.com> Hi Gudrun: I recommend you to get "The Microwave tool book" by Login and Dvorak (1994) I am also sending you under separate cover an article I wrote on the subject. As to your questions, the practice of histology has concluded that: 1- the physical principle is that microwaves excite ("shake") all chemical molecules with electrical charge and, in consequence, that "shaking"?produces heat. That is why paraffin and any "non-polar" molecule cannot be heated in a MW oven per se. 2- infiltration is faster because the heat is generated within the tissues, not by external convection 3- proteins (and antigens as proteins themselves) are not adversely affected by MW radiation (or so the say). 4- everybody using MW tissue processing claims that IHC procedures are not affected by the procedure. Having said all of the above I personally do not like MW processing; there are many ways of having fast processing with conventional tissue processors. Ren? J. ________________________________ From: Gudrun Lang To: Histonet@lists.utsouthwestern.edu Sent: Monday, January 13, 2014 1:56 PM Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kalschev <@t> svm.vetmed.wisc.edu Mon Jan 13 16:05:27 2014 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Mon Jan 13 16:05:34 2014 Subject: [Histonet] Buehler Isomet 2000 Precision Saw or later model Message-ID: <2090328140564B968CEBD13ABADA6EDC@vetmed.wisc.edu> Actively looking to purchase - possibly two, in good condition. Thanks Vicki @ 608-262-8534 From tony.henwood <@t> health.nsw.gov.au Mon Jan 13 18:26:18 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jan 13 18:26:32 2014 Subject: [Histonet] myeloperoxidase on FFPE or frozen sections In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157D61B2453@xmdb01.nch.kids> Yep, We use a rtu from Leica on the Bond3 platform (PA0491) with EDTA (high pH) antigen retrieval Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mesru T Sent: Tuesday, 14 January 2014 5:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] myeloperoxidase on FFPE or frozen sections Dear Histonetters, I wonder if anyone has done myeloperoxidase stain on FFPE or frozen lung sections? I would appreciate any help. Regards, Mesru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From nguy0515 <@t> gmail.com Mon Jan 13 18:28:08 2014 From: nguy0515 <@t> gmail.com (Trini) Date: Mon Jan 13 18:28:13 2014 Subject: [Histonet] Recuts/deepers Message-ID: <1CA11511-352C-4788-8700-9BE2CB0751AF@gmail.com> Hello histonetters! Could I please get some help/advice/ideas on how to reduce recut/deeper requests? What does your lab do to reduce that? Would you say embedding is partially the problem? Such as small tissue embedded with bigger tissue? Etc? From Timothy.Morken <@t> ucsfmedctr.org Mon Jan 13 22:36:29 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Jan 13 22:36:41 2014 Subject: [Histonet] Recuts/deepers In-Reply-To: <1CA11511-352C-4788-8700-9BE2CB0751AF@gmail.com> References: <1CA11511-352C-4788-8700-9BE2CB0751AF@gmail.com> Message-ID: <761E2B5697F795489C8710BCC72141FF367432D5@ex07.net.ucsf.edu> What are the reasons they ask for recuts? The answer to that will give you some ideas. For instance, did they not get a full face with margins the first time? Was the section not readable? Was the stain inadequate? Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Trini Sent: Monday, January 13, 2014 4:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recuts/deepers Hello histonetters! Could I please get some help/advice/ideas on how to reduce recut/deeper requests? What does your lab do to reduce that? Would you say embedding is partially the problem? Such as small tissue embedded with bigger tissue? Etc? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From irena.kirbis <@t> hotmail.com Tue Jan 14 05:03:15 2014 From: irena.kirbis <@t> hotmail.com (IRENA) Date: Tue Jan 14 05:03:21 2014 Subject: [Histonet] IHC control slides Message-ID: Hi, there is disagreement at the our dept regarding the control slides for IHC, so far on the control slides there is only writen control, marker and date of staining (on automated stainer) but there is NOT indicated/writen the ID of control, however I believe or it's obvious to me that is necessary/mandatory to write and document properly also the ID of control to be able to follow the performance of each individual control over time at least, looking forward to hear any other argument to support (or not) my feeling, is there any reference stating how should be control slides documented/stored etc. I think that it's not enough just to have any positive control present but you should also be able to demonstrate at any time which control did you use, how many times and what was the performance of each control? many thanks in advance Irena From tmcampbell <@t> fmh.org Tue Jan 14 08:55:24 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Tue Jan 14 08:55:24 2014 Subject: [Histonet] Warthin Starry Help Message-ID: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining. I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampbell@fmh.org From rjr6 <@t> psu.edu Tue Jan 14 09:10:54 2014 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Tue Jan 14 09:11:11 2014 Subject: [Histonet] RE: Warthin Starry Help In-Reply-To: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> References: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> Message-ID: I am also in a small lab and this is how I do our Warthin Starry. I usually only have 1 or 2 slides to stain at a time and I stain them in a (brand new never before used for anything) plastic 5-slide mailer. I mix the reagents in disposable 20 ml beakers. I stain the slides using my flotation bath turned all the way up. I put the acidulated water in the water bath. Then I measure out the Silver Nitrate in the mailer and mix it with the acidulated water then I measure the hydroquonine, gelatin, and the silver nitrate for the developer in the 20 ml beakers. I add water to the gelatin only at this point an put in between the glass rods on the water bath so it will warm up and dissolve the gelatin. When the impregnation with the silver nitrate is finished then I mix the hydroquinone and the developer silver nitrate. I lay the slides on the rods over the water bath then flood the sections with the developer. Rinse in hot water and dehydrate etc. The only time I have had problems is in the winter. My lab is poorly insulated and the water bath sets by the window so I take slide folders and make a wind break. I hope this helps. I have tried the microwave method and could never get it to work. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Tuesday, January 14, 2014 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin Starry Help I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining. I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampbell@fmh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 14 09:11:15 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 14 09:11:20 2014 Subject: [Histonet] Warthin Starry Help In-Reply-To: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> References: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> Message-ID: <1389712275.25548.YahooMailNeo@web120406.mail.ne1.yahoo.com> Although many histotechs successfully use?the Warhin Starry stain (or so they say!) I find it the most unreliable and capricious of all stains, so much so that I stopped using it many years ago and started using the modified Steiner that stains H.pylory and all Treponema and related bacteria. The only alleged drawback of the Steiner stain is obtaining uranyl nitrate and its low radioactivity. To eliminate that problem I substituted it with phosphotungstic solution. Under separate cover I am sending you my modified Steiner method with phosphotungstic acid. Another thing, I commend you for preparing your own reagents. You belong to a "dying breed" of histotechs. Ren? J. ________________________________ From: "Campbell, Tasha M." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 14, 2014 9:55 AM Subject: [Histonet] Warthin Starry Help I need help with warthin starry stain please.? I am a very small lab trying to save money so I want to make the reagents myself.? I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started.? Then suddenly it quit working and I cannot get it to work again.? I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water.? The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining.? I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampbell@fmh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Jan 14 09:16:19 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jan 14 09:28:32 2014 Subject: [Histonet] Warthin Starry Help In-Reply-To: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> Message-ID: <7898356D-FB2C-4142-A0AA-A2081D97EB94@mtsac.edu> Hydroquinone has a short shelf life once opened. > On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." wrote: > > I need help with warthin starry stain please. I am a very small lab > trying to save money so I want to make the reagents myself. I have > sigma Aldrich dry reagents and have been making the reagents and > following the instructions correctly and I did get the stain to work > wonderfully when I first started. Then suddenly it quit working and I > cannot get it to work again. I have made fresh reagents day after day > and I have a pH of 3.8 for the acidulated water. The tissue will stain > normally with the yellow to brown background and I the developing > solution turns black when it is left on paper but the h.pylori organisms > are not staining. I am getting very frustrated and need some help > please. > > > > Thank you. > > > > Tasha Campbell, HTL > > tmcampbell@fmh.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbell <@t> fmh.org Tue Jan 14 09:36:29 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Tue Jan 14 09:36:35 2014 Subject: [Histonet] Warthin Starry Help In-Reply-To: <7898356D-FB2C-4142-A0AA-A2081D97EB94@mtsac.edu> References: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> <7898356D-FB2C-4142-A0AA-A2081D97EB94@mtsac.edu> Message-ID: <3566D9E34287BE4B95372179009446A019213E77@EXCHANGE.fmhnt.fmh.org> I was wondering about that. Even in its crystal form? I've been trying to test every possible problem, though, and I am using a kit right now so I have substituted the kit's hydroquinone in place of mine and used the rest of my reagents. It still didn't work. So this has to mean there is another problem. But with the entire kit's reagents it works. I have narrowed down by substituting my reagents in for the kit's one by one and the only combination I can get to work is using my 1% silver for impregnation and then the kit's reagents for developing so this has to mean that my silver is good. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Tuesday, January 14, 2014 10:16 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry Help Hydroquinone has a short shelf life once opened. > On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." wrote: > > I need help with warthin starry stain please. I am a very small lab > trying to save money so I want to make the reagents myself. I have > sigma Aldrich dry reagents and have been making the reagents and > following the instructions correctly and I did get the stain to work > wonderfully when I first started. Then suddenly it quit working and I > cannot get it to work again. I have made fresh reagents day after day > and I have a pH of 3.8 for the acidulated water. The tissue will stain > normally with the yellow to brown background and I the developing > solution turns black when it is left on paper but the h.pylori organisms > are not staining. I am getting very frustrated and need some help > please. > > > > Thank you. > > > > Tasha Campbell, HTL > > tmcampbell@fmh.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Tue Jan 14 09:52:41 2014 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Tue Jan 14 09:53:06 2014 Subject: [Histonet] Warthin Starry Help In-Reply-To: <7898356D-FB2C-4142-A0AA-A2081D97EB94@mtsac.edu> References: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> <7898356D-FB2C-4142-A0AA-A2081D97EB94@mtsac.edu> Message-ID: My hydroquonine was received in the lab in 1980 and it works. As long as my control is positive I am allowed to keep it but then I work in a veterinary diagnostic lab and am not under all the regulations that those of you in hospitals are under. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, January 14, 2014 10:16 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry Help Hydroquinone has a short shelf life once opened. > On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." wrote: > > I need help with warthin starry stain please. I am a very small lab > trying to save money so I want to make the reagents myself. I have > sigma Aldrich dry reagents and have been making the reagents and > following the instructions correctly and I did get the stain to work > wonderfully when I first started. Then suddenly it quit working and I > cannot get it to work again. I have made fresh reagents day after day > and I have a pH of 3.8 for the acidulated water. The tissue will > stain normally with the yellow to brown background and I the > developing solution turns black when it is left on paper but the > h.pylori organisms are not staining. I am getting very frustrated and > need some help please. > > > > Thank you. > > > > Tasha Campbell, HTL > > tmcampbell@fmh.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Margraf <@t> cookchildrens.org Tue Jan 14 10:12:24 2014 From: Linda.Margraf <@t> cookchildrens.org (Linda Margraf) Date: Tue Jan 14 10:12:33 2014 Subject: [Histonet] warthin starry problems Message-ID: <928719B9EBFA1C4686918B975FF84528EDE2A286@CCHCSMBX04.CCHCS.LDAP> Dear Histonetters: Here is a message I am posting from Tasha...she hasn't yet joined the list but is eager for answers to her question. I will forward answers to her or you can send them directly to her at tmcampbell@fmh.org "I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. I am getting very frustrated and need some help please." Thanks Linda M Histonet administrator From tmcampbell <@t> fmh.org Tue Jan 14 10:14:15 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Tue Jan 14 10:14:14 2014 Subject: [Histonet] RE: warthin starry problems In-Reply-To: <928719B9EBFA1C4686918B975FF84528EDE2A286@CCHCSMBX04.CCHCS.LDAP> References: <928719B9EBFA1C4686918B975FF84528EDE2A286@CCHCSMBX04.CCHCS.LDAP> Message-ID: <3566D9E34287BE4B95372179009446A019213F0F@EXCHANGE.fmhnt.fmh.org> Im sorry Linda! I got myselft added and posted it and I have already had feedback! Thank you though. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) ________________________________ From: Linda Margraf [mailto:Linda.Margraf@cookchildrens.org] Sent: Tuesday, January 14, 2014 11:12 AM To: 'histonet@lists.utsouthwestern.edu' Cc: Campbell, Tasha M. Subject: warthin starry problems Dear Histonetters: Here is a message I am posting from Tasha...she hasn't yet joined the list but is eager for answers to her question. I will forward answers to her or you can send them directly to her at tmcampbell@fmh.org "I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. I am getting very frustrated and need some help please." Thanks Linda M Histonet administrator From akbitting <@t> geisinger.edu Tue Jan 14 10:36:34 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Jan 14 10:36:44 2014 Subject: [Histonet] Recuts/deepers In-Reply-To: <761E2B5697F795489C8710BCC72141FF367432D5@ex07.net.ucsf.edu> References: <1CA11511-352C-4788-8700-9BE2CB0751AF@gmail.com> <761E2B5697F795489C8710BCC72141FF367432D5@ex07.net.ucsf.edu> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F65D3DAFBA@GHSEXMBX1W8K1V.geisinger.edu> We track "shallow/incomplete" sections and have found numerous reasons. Inadequate fixation. The way a tissue is bisected can cause it to not lay flat in the embedding mold. For anstance a grossing tech who does a "wrist turn" kind of slicing motion and causes an arch shaped edge. Embedding pieces that are varying sizes. Too many pieces in a cassette. Some Histotechs are trained to err on the side of caution and do not like to cut too deep the first time. Their though being you can always come back and recut it, but once it's cut away, it's gone. Our idea was that by tracking these we might find an opportunity for retraining an individual, but as it turns out, we all cut a shallow/incomplete section occasionally. Some steps we took - We had the grossing techs come and watch us embed derm cases. That gave them a perspective of why it's important for them to do things a certain way. One of our Dermatopathologists came in and gave the HTs and in-service on what they are looking to see on a slide and what the inked margins mean. This helped with orienting specimens at embedding. We have reduced our recut numbers by taking the time to identify the causes and offering additional training to the staff. Collecting the data is the first step, and though it's time-consuming, it is worth it in the end. Best wishes. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, January 13, 2014 11:36 PM To: Trini; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Recuts/deepers What are the reasons they ask for recuts? The answer to that will give you some ideas. For instance, did they not get a full face with margins the first time? Was the section not readable? Was the stain inadequate? Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Trini Sent: Monday, January 13, 2014 4:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recuts/deepers Hello histonetters! Could I please get some help/advice/ideas on how to reduce recut/deeper requests? What does your lab do to reduce that? Would you say embedding is partially the problem? Such as small tissue embedded with bigger tissue? Etc? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From workshopihc <@t> gmail.com Tue Jan 14 10:41:40 2014 From: workshopihc <@t> gmail.com (Workshop IHC) Date: Tue Jan 14 10:41:46 2014 Subject: [Histonet] IHC wet workshop anouncement Message-ID: Learn IHC staining, Hands - on IHC Lab course....CEU approved; February 27th & 28th, 2014 San Francisco Bay area. For more info contact Maria workshopihc@gmail.com From anolan <@t> prometheushealthcare.com Tue Jan 14 10:45:20 2014 From: anolan <@t> prometheushealthcare.com (anolan@prometheushealthcare.com) Date: Tue Jan 14 10:43:06 2014 Subject: [Histonet] IHC Tech San Francisco Area Message-ID: <024701cf1148$04ae19f0$0e0a4dd0$@prometheushealthcare.com> Hi All, I'm currently recruiting for a new IHC tech with a facility located in San Francisco, CA. Please find job description below: IHC Histotechnologist/Histotechnician Our Client is an exciting, high-growth molecular clinical laboratory located in the Bay Area (Northern CA), utilizing cutting-edge technology. The laboratory is CLIA-certified and CAP-accredited. Our Client has full-time, part-time and per diem openings for a Histotechnologist or Histotechnician, preferably with an emphasis in immunohistochemistry. Candidate is responsible for performing routine, special and immunohistochemistry stains, as well as embedding and sectioning of pathology specimens and mounting, labeling and distributing microscopy slides to the Molecular lab and the Pathologists. The ideal candidate will have: . Minimum two (2) years of experience as a Histotechnologist or Histotechnician, preferably in immunohistochemistry . In-depth knowledge and understanding of all technical aspects of the areas of Histology and Pathology, laboratory techniques, scientific principles and quality control . Ability to embed, cut, stain and mount human tissue specimens following established policies and standards . Ability to use microtome and mount micro-thin tissue sections onto microscope slides . Strong organizational and computer skills Licensure and Education requirement: . Histolotechnologist (HTL) (ASCP) or Histotechnician (HT) license strongly preferred, but not required . Bachelor's Degree in Science or related field . Must pass a Color/Vision Test Anna Nolan - Recruiter Prometheus Healthcare Direct Line 301-693-8908 Office 301-693-9057 Fax 301-368-2478 anolan @prometheushealthcare.com http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 www.prometheushealthcare.com From Lynn.Burton <@t> Illinois.gov Tue Jan 14 10:44:41 2014 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Jan 14 10:45:32 2014 Subject: [Histonet] Warthin Starry Help In-Reply-To: References: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> <7898356D-FB2C-4142-A0AA-A2081D97EB94@mtsac.edu> Message-ID: Same here. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Tuesday, January 14, 2014 9:53 AM To: Jennifer MacDonald; Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Warthin Starry Help My hydroquonine was received in the lab in 1980 and it works. As long as my control is positive I am allowed to keep it but then I work in a veterinary diagnostic lab and am not under all the regulations that those of you in hospitals are under. Roberta Horner HT/HTL Animal Diagnostic Lab Penn State University -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, January 14, 2014 10:16 AM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry Help Hydroquinone has a short shelf life once opened. > On Jan 14, 2014, at 6:59 AM, "Campbell, Tasha M." wrote: > > I need help with warthin starry stain please. I am a very small lab > trying to save money so I want to make the reagents myself. I have > sigma Aldrich dry reagents and have been making the reagents and > following the instructions correctly and I did get the stain to work > wonderfully when I first started. Then suddenly it quit working and I > cannot get it to work again. I have made fresh reagents day after day > and I have a pH of 3.8 for the acidulated water. The tissue will > stain normally with the yellow to brown background and I the > developing solution turns black when it is left on paper but the > h.pylori organisms are not staining. I am getting very frustrated and > need some help please. > > > > Thank you. > > > > Tasha Campbell, HTL > > tmcampbell@fmh.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Tue Jan 14 10:51:03 2014 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Jan 14 10:51:18 2014 Subject: [Histonet] RE: Techs grossing FS tissue In-Reply-To: References: <20140113172526.46F481E8060@trendmess-svr.holyredeemer.local> Message-ID: <5BB119AC-01E9-4239-A33E-C40075BF8D48@yahoo.com> Agreed. They can plop it on the block, cut it , stain it but they can't measure it, dissect it or ink it as those task are considered gross dissection which is high complexity and the person doing them has to meet CLIA guidelines for high complexity. Sent from my iPhone On Jan 13, 2014, at 2:20 PM, "Terri Braud" wrote: > This is the only way that a non-pathologist can gross tissue, whether > for frozen section or otherwise. They must meet CLIA standards for high > complexity testing and furthermore, CAP says that the exact nature of > the tissue grossed must be spelled out, along with nature of the > pathologists' supervision (direct, indirect), and have at least annual > competencies on grossing on file. That's the basic rules. State > licensing may possibly add, but never subtract to this. > However, there is nothing that says a tech can't plop a small singular > piece of tissue onto a chuck to freeze and get things started. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > > Message: 3 > Date: Mon, 13 Jan 2014 15:34:48 +0000 > From: "Leann M. Murphy" > Good morning, > I know all histotechs help cut and stain frozen sections. Is there any > organization out there that has the histotechnician gross the frozen > section tissue and place on the frozen section chuck to cut without the > Pathologist in the room. If anyone does this please tell me why? And > if your organization doesn't do this please tell me why. I am having a > debate with our Pathologists over this question. > > Thank You, > LeAnn Murphy > Aultman Hospital > Canton, Ohio > > > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it was sent. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Millard <@t> prlnet.com Tue Jan 14 11:01:28 2014 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Tue Jan 14 11:01:34 2014 Subject: Subject: [Histonet] Recuts/deepers Message-ID: <64D11CD058311340A1966C234C6B0B8927E67037@PRLEXCH02.prlnet.com> I have actually had some success in reducing recuts, and attribute to a couple of things. 1. Are pathologists are now measured on a case TAT without any exceptions. (before they could exclude cases for additional work/stains-(including recuts), held for fixation, decal...). They are ordering fewer recuts. 2. I do a monthly report on Recuts, and look at the number of recuts by part-type, sectioning tech and pathologist. I try to determine which recuts are due to tech error from the comment that populates with the order (i.e. the comment on the order says "need complete surface" vs "following atypical focus". I figure if it's levels over 2, the tech was not going to get it in one slide to begin with). Note there is a level of subjectivity here, and I probably err on the side of blaming the tech-but I do the best I can, and it does give information back. The techs get fed back this information and can see what the % of blocks they cut that require recuts, and what type of tissue. If a pathologist is consistently ordering an unusually high number of recuts (particularly compared to other pathologists doing similar work), that gets fed back to Chief Medical officer and Medical Director. We had a case where one pathologist was ordering 12% versus everyone else ordering about 3-4% or less and was responsible for 30-40% of all recuts-that has been dramatically reduced). And yes, I do think a fair number of recuts may actually be due to embedding error-we are trying to put processes in place to capture this, and get techs to re-embed and note this to be fed back as well. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From gu.lang <@t> gmx.at Tue Jan 14 11:53:37 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jan 14 11:53:43 2014 Subject: AW: [Histonet] microwave processing In-Reply-To: <000001cf1091$21344610$639cd230$@gmx.at> References: <000001cf1091$21344610$639cd230$@gmx.at> Message-ID: <001401cf1151$8d782c40$a86884c0$@gmx.at> Thank you for the kind responses. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Montag, 13. J?nner 2014 19:56 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.LaFriniere <@t> ccplab.com Tue Jan 14 12:45:49 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Tue Jan 14 12:46:01 2014 Subject: [Histonet] RE: Frozen sections In-Reply-To: References: Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2964BC51@AHCMSASEXCH03.my.ahc.local> Hi Leann, Dependant on the case and how much tissue comes in from the OR, In my many years...I have on many occasions grossed and placed tissue for FS on chuck, and performed the frozen section, had it under the scope looking at it prior to the Pathologist coming in for DX.(why) because we have experience and it's a comfort level the pathologists under their guidance and supervision, Many of us have the experience to do this especially if you are grandfathered or have the required education under the CLIA regs to gross. I don't know of any regulation other than what I have mentioned above that would not allow the experienced HT to perform this operation. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Monday, January 13, 2014 10:35 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen sections Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Tue Jan 14 13:26:06 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Jan 14 13:26:11 2014 Subject: Subject: [Histonet] Recuts/deepers In-Reply-To: <20140114172502.165261E8062@trendmess-svr.holyredeemer.local> References: <20140114172502.165261E8062@trendmess-svr.holyredeemer.local> Message-ID: We, too, had an excess of recuts and deepers. After careful study, it was found the majority were due to a preference for certain pathologists, but many were due to poor grossing / poor processing, and a few were due to tech error with tech error being the least number of the categories We tackled the reduction one group at a time. Previously, we cut GI's at 2 levels, but a slight majority of the pathologists preferred more. To keep the number of slides low, but give the extra levels desired, we now cut 3 levels of all small biopsies, mounted on one slide. For LEEPs, we upped the number of original levels, too. The pathologists also agreed to cut and fix any large specimen overnight, since there was no negative impact on patient care. Now, our larger specimens gross more easily, and consequently embed and cut better. To correct the grossing problem, the techs and PA had a an inservice during embedding so that the PA could see the effects of the grossing issues we were dealing with. Out techs and PA came up with a new inking system that not only identified skin margins as needed, but also indicated "tips up with a red dot" for the techs, eliminating all incorrectly embedded skins. The techs were also able to demonstrate to the PA, the importance of thickness consistency for serial sections embedded together. Our effort was a group effort for the pathologists, the PA, and the technical staff all cooperating and our recut/deepers orders were reduced by over 70%, much greater than our original target. This was 6 months ago, and our numbers are still holding. Best of Luck Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 *********************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Michael.LaFriniere <@t> ccplab.com Tue Jan 14 13:32:56 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Tue Jan 14 13:33:03 2014 Subject: [Histonet] RE: error rate for sectioning In-Reply-To: <997D36BD8D0ACF418AF900F4E781DE4414BCB4D6@MLBXv03.nih.gov> References: <997D36BD8D0ACF418AF900F4E781DE4414BCB4D6@MLBXv03.nih.gov> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2964BCE8@AHCMSASEXCH03.my.ahc.local> Hi Patricia, Error rates should not be set by employers. You should have an effective QA management program and committee even in small practices with the Medical Director as the Chair. The committee sets and establishes error rate thresholds with monthly monitors in place to seek opportunities of improvement should error rates exceed below the set threshold. Hope this helps! Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zerfas, Patricia (NIH/OD/ORS) [E] Sent: Saturday, January 11, 2014 4:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] error rate for sectioning Hi All, What is the error rate set by you or your employer for paraffin sectioning? These are slides that are found to be unacceptable due to wrinkling, folding etc either by a pathologist or another reviewer. I was found to have 6 slides out of approx 1500 that I have sectioned to date to be unacceptable. I only perform this duty when I have completed all of my other work so this is not one of my regular duties. At times I have gone several months without sectioning and utilize a microtome that is approx. 26 years old. Since this was brought to my attention I have been given more up to date equipment which I have yet to utilize. Thanks in advance for your feedback. Patricia Zerfas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carol.Freeman <@t> utoledo.edu Tue Jan 14 14:30:06 2014 From: Carol.Freeman <@t> utoledo.edu (Freeman, Carol) Date: Tue Jan 14 14:30:29 2014 Subject: [Histonet] RE: Histonet Digest, Vol 122, Issue 17 In-Reply-To: <4b1ef84e-b949-4cb2-9bae-81aa5fd577ef@MsgApp11.utad.utoledo.edu> References: <4b1ef84e-b949-4cb2-9bae-81aa5fd577ef@MsgApp11.utad.utoledo.edu> Message-ID: <798854C89D423345A4AC8F643E5D23771BF24ECA@msgdb20.utad.utoledo.edu> In response to message 4 what is considered the requirements for high complexity testing for grossing... is that a bachelor's degree with pathologist guidance or do they have to have a certificate of completion for being a P.A. (ASCP)..... _________________________________________________________________________________________________________________________________________________________________________ Message: 4 Date: Tue, 14 Jan 2014 11:51:03 -0500 From: Kim Donadio Subject: Re: [Histonet] RE: Techs grossing FS tissue To: Terri Braud Cc: "" Message-ID: <5BB119AC-01E9-4239-A33E-C40075BF8D48@yahoo.com> Content-Type: text/plain; charset=us-ascii Agreed. They can plop it on the block, cut it , stain it but they can't measure it, dissect it or ink it as those task are considered gross dissection which is high complexity and the person doing them has to meet CLIA guidelines for high complexity. Sent from my iPhone On Jan 13, 2014, at 2:20 PM, "Terri Braud" wrote: > This is the only way that a non-pathologist can gross tissue, whether > for frozen section or otherwise. They must meet CLIA standards for high > complexity testing and furthermore, CAP says that the exact nature of > the tissue grossed must be spelled out, along with nature of the > pathologists' supervision (direct, indirect), and have at least annual > competencies on grossing on file. That's the basic rules. State > licensing may possibly add, but never subtract to this. > However, there is nothing that says a tech can't plop a small singular > piece of tissue onto a chuck to freeze and get things started. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > > Message: 3 > Date: Mon, 13 Jan 2014 15:34:48 +0000 > From: "Leann M. Murphy" > Good morning, > I know all histotechs help cut and stain frozen sections. Is there any > organization out there that has the histotechnician gross the frozen > section tissue and place on the frozen section chuck to cut without the > Pathologist in the room. If anyone does this please tell me why? And > if your organization doesn't do this please tell me why. I am having a > debate with our Pathologists over this question. > > Thank You, > LeAnn Murphy > Aultman Hospital > Canton, Ohio From Bruce_Palmatier <@t> vwr.com Tue Jan 14 15:02:01 2014 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Tue Jan 14 15:02:23 2014 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 01/22/2014) Message-ID: I am out of the office until 01/22/2014. I will be out of the office from January 13th- January 22nd. I will be checking emails periodically and will attempt to reply to your message within 24 hours. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 122, Issue 15" sent on 1/14/2014 2:46:03 PM. This is the only notification you will receive while this person is away. From Tony.Reilly <@t> health.qld.gov.au Tue Jan 14 18:38:24 2014 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Tue Jan 14 18:38:40 2014 Subject: [Histonet] RE: Warthin Starry Help In-Reply-To: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> References: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> Message-ID: Hi Tasha I have been performing the Warthin-Starry successfully for nearly 40 years. Without seeing your method these are a few tips that should be followed. 1. Make sure all solutions are made fresh each time using an acetate buffer or acidulated water as diluent. I prefer an acetate buffer. 2. Because of the difficulty in controlling development with this method, more than one section and control should be stained, with shorter and longer development times. 3. Sections must not be allowed to dry before flooding with the developer solution, as this will cause precipitation of silver, making results difficult to interpret. 4. Do not wash slides between pouring off the silver solution and adding the developer. 5. Ensure that the developer is made up just before use and is up to temperature. Add silver nitrate to dissolved gelatin, stirring continuously. Ensure this is done slowly to prevent the formation of precipitate. Just before time of use, add hydroquinone. 6. Allow sections on the first pair of slides to develop until the background appears yellow to light golden brown. This will take approximately 5-7 minutes. Allow the second pair of slides to develop until the background appears brown to dark brown. This may take up to an additional 3-5 minutes. Regular microscopic examination is required to check for reaction endpoints. Flood slides with acetate buffer or acidulated water once endpoints are reached to prevent further silver development. 7. Fixatives containing chrome and mercuric salts should not be used as they can interfere with the reaction, giving false negative results. 8. Discard developer once it becomes too dark. If sections are underdeveloped they can be placed into a second batch of fresh developer solution for another 3-5 minutes. 9. The method will demonstrate a number of argyrophilic organisms. If a particular infectious agent is suspected, use a control section containing that organism. If the cause of infection is in doubt, use a control containing Spirochaetes. If this is successfully impregnated it can virtually be assumed that all other argyrophilic organisms would be demonstrated. 10. Melanin and other argentaffin or argyrophilic substances may also be demonstrated. 11. Some methods wash in hot tap water following development, I prefer to rinse in hot acetate buffer or acidulated water first to avoid any background precipitation of silver. I hope this is of some use. Regards Tony Tony Reilly? B.App.Sc. ,?M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency |?Department of?Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA? Qld 4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony.reilly@health.qld.gov.au Web:? www.health.qld.gov.au/qhcss/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tasha M. Campbell Sent: Wednesday, 15 January 2014 12:55 AM To: Tony Reilly; histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin Starry Help I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining. I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampbell@fmh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From ahc53 <@t> georgetown.edu Wed Jan 15 07:04:01 2014 From: ahc53 <@t> georgetown.edu (Anna Coffey) Date: Wed Jan 15 07:04:10 2014 Subject: [Histonet] 1. IHC wet workshop announcement (Workshop IHC) Message-ID: Does anyone know of any similar IHC courses on the east coast? I'd love to attend this one, but won't be able to swing the travel costs. Thanks! Message: 1 Date: Tue, 14 Jan 2014 08:41:40 -0800 From: Workshop IHC Subject: [Histonet] IHC wet workshop anouncement To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Learn IHC staining, Hands - on IHC Lab course....CEU approved; February 27th & 28th, 2014 San Francisco Bay area. For more info contact Maria workshopihc@gmail.com -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu From juan.l.bassett.ctr <@t> mail.mil Wed Jan 15 07:53:44 2014 From: juan.l.bassett.ctr <@t> mail.mil (Bassett, Juan L CTR (US)) Date: Wed Jan 15 07:53:04 2014 Subject: [Histonet] FW: Frozen sections (UNCLASSIFIED) Message-ID: <5BEFD2C58946394F8E2A6EC3E1F86A9B0161BE01@umechpha.easf.csd.disa.mil> Classification: UNCLASSIFIED Caveats: NONE Hi LeAnn, Please note my response below. -----Original Message----- From: Bassett, Juan L CTR (US) Sent: Wednesday, January 15, 2014 7:39 AM To: 'Michael LaFriniere' Cc: 'stonet-bounces@lists.utsouthwestern.edu' Subject: RE: Frozen sections (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Michael, Based on my experience the pathologist or pathologists asst. are qualified to submit the specimen to be sectioned during a Frozen section procedure. And the pathologist asst. is allowed with the pathologist's approval/acknowledgement. Qualified HT and HTL's can gross non complicated specimens within normal tissue grossing procedures. There could be legal issues involved with a technician's unilateral decision during a frozen section procedure! To get more accurate feedback on this topic , I suggest you contact the ASCP with your consideration. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael LaFriniere Sent: Tuesday, January 14, 2014 1:46 PM To: Leann M. Murphy; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Frozen sections Hi Leann, Dependant on the case and how much tissue comes in from the OR, In my many years...I have on many occasions grossed and placed tissue for FS on chuck, and performed the frozen section, had it under the scope looking at it prior to the Pathologist coming in for DX.(why) because we have experience and it's a comfort level the pathologists under their guidance and supervision, Many of us have the experience to do this especially if you are grandfathered or have the required education under the CLIA regs to gross. I don't know of any regulation other than what I have mentioned above that would not allow the experienced HT to perform this operation. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Monday, January 13, 2014 10:35 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen sections Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE Classification: UNCLASSIFIED Caveats: NONE From Michael.LaFriniere <@t> ccplab.com Wed Jan 15 08:11:01 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Wed Jan 15 08:11:13 2014 Subject: [Histonet] RE: Frozen sections (UNCLASSIFIED) In-Reply-To: <5BEFD2C58946394F8E2A6EC3E1F86A9B0161BE01@umechpha.easf.csd.disa.mil> References: <5BEFD2C58946394F8E2A6EC3E1F86A9B0161BE01@umechpha.easf.csd.disa.mil> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D2964BDEF@AHCMSASEXCH03.my.ahc.local> Hi Juan, I am not sure of any ASCP guidelines nor jurisdiction over CLIA requirements. It is my understanding all US laboratories have to follow the CLIA set guidelines for performing all laboratory testing. Qualified HT or HTLs can gross complicated (high Complexity) testing under specific CLIA guidelines. CLIA guidelines have a grandfather clause for high complexity testing of experienced techs prior to (I think the exact date is) Sept 1996 that can demonstrate experience of the high complexity testing. Following Sept 1996 the educational guidelines set in for any high complexity laboratory testing requirements. Michael -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bassett, Juan L CTR (US) Sent: Wednesday, January 15, 2014 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Frozen sections (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Hi LeAnn, Please note my response below. -----Original Message----- From: Bassett, Juan L CTR (US) Sent: Wednesday, January 15, 2014 7:39 AM To: 'Michael LaFriniere' Cc: 'stonet-bounces@lists.utsouthwestern.edu' Subject: RE: Frozen sections (UNCLASSIFIED) Classification: UNCLASSIFIED Caveats: NONE Michael, Based on my experience the pathologist or pathologists asst. are qualified to submit the specimen to be sectioned during a Frozen section procedure. And the pathologist asst. is allowed with the pathologist's approval/acknowledgement. Qualified HT and HTL's can gross non complicated specimens within normal tissue grossing procedures. There could be legal issues involved with a technician's unilateral decision during a frozen section procedure! To get more accurate feedback on this topic , I suggest you contact the ASCP with your consideration. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael LaFriniere Sent: Tuesday, January 14, 2014 1:46 PM To: Leann M. Murphy; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Frozen sections Hi Leann, Dependant on the case and how much tissue comes in from the OR, In my many years...I have on many occasions grossed and placed tissue for FS on chuck, and performed the frozen section, had it under the scope looking at it prior to the Pathologist coming in for DX.(why) because we have experience and it's a comfort level the pathologists under their guidance and supervision, Many of us have the experience to do this especially if you are grandfathered or have the required education under the CLIA regs to gross. I don't know of any regulation other than what I have mentioned above that would not allow the experienced HT to perform this operation. Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Monday, January 13, 2014 10:35 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen sections Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE Classification: UNCLASSIFIED Caveats: NONE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbell <@t> fmh.org Wed Jan 15 08:14:14 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Wed Jan 15 08:14:27 2014 Subject: [Histonet] RE: Warthin Starry Help In-Reply-To: References: <3566D9E34287BE4B95372179009446A0190698E8@EXCHANGE.fmhnt.fmh.org> Message-ID: <3566D9E34287BE4B95372179009446A019214627@EXCHANGE.fmhnt.fmh.org> Thank you so much. These tips seem very helpful. Could you give me your protocol? Times in temps in the impregnation step. And your measurements for each reagent in the developing solution. I use acidulated water and I usually get the pH at about 3.8. I am staining for h.Pylori. Like I said, when I first started making my own reagents, I actually made up enough of each for a week and the stain was working beautifully. But after a couple months, it has just mysteriously stopped working. I just don't get it. I can use a kit and the sections will stain wonderfully but just cant get my own reagents to work and I am trying to save money since I am such a small lab. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: Tony Reilly [mailto:Tony.Reilly@health.qld.gov.au] Sent: Tuesday, January 14, 2014 7:38 PM To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu Subject: RE: Warthin Starry Help Hi Tasha I have been performing the Warthin-Starry successfully for nearly 40 years. Without seeing your method these are a few tips that should be followed. 1. Make sure all solutions are made fresh each time using an acetate buffer or acidulated water as diluent. I prefer an acetate buffer. 2. Because of the difficulty in controlling development with this method, more than one section and control should be stained, with shorter and longer development times. 3. Sections must not be allowed to dry before flooding with the developer solution, as this will cause precipitation of silver, making results difficult to interpret. 4. Do not wash slides between pouring off the silver solution and adding the developer. 5. Ensure that the developer is made up just before use and is up to temperature. Add silver nitrate to dissolved gelatin, stirring continuously. Ensure this is done slowly to prevent the formation of precipitate. Just before time of use, add hydroquinone. 6. Allow sections on the first pair of slides to develop until the background appears yellow to light golden brown. This will take approximately 5-7 minutes. Allow the second pair of slides to develop until the background appears brown to dark brown. This may take up to an additional 3-5 minutes. Regular microscopic examination is required to check for reaction endpoints. Flood slides with acetate buffer or acidulated water once endpoints are reached to prevent further silver development. 7. Fixatives containing chrome and mercuric salts should not be used as they can interfere with the reaction, giving false negative results. 8. Discard developer once it becomes too dark. If sections are underdeveloped they can be placed into a second batch of fresh developer solution for another 3-5 minutes. 9. The method will demonstrate a number of argyrophilic organisms. If a particular infectious agent is suspected, use a control section containing that organism. If the cause of infection is in doubt, use a control containing Spirochaetes. If this is successfully impregnated it can virtually be assumed that all other argyrophilic organisms would be demonstrated. 10. Melanin and other argentaffin or argyrophilic substances may also be demonstrated. 11. Some methods wash in hot tap water following development, I prefer to rinse in hot acetate buffer or acidulated water first to avoid any background precipitation of silver. I hope this is of some use. Regards Tony Tony Reilly? B.App.Sc. ,?M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory ________________________________________________ Health Services Support Agency |?Department of?Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA? Qld 4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony.reilly@health.qld.gov.au Web:? www.health.qld.gov.au/qhcss/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tasha M. Campbell Sent: Wednesday, 15 January 2014 12:55 AM To: Tony Reilly; histonet@lists.utsouthwestern.edu Subject: [Histonet] Warthin Starry Help I need help with warthin starry stain please. I am a very small lab trying to save money so I want to make the reagents myself. I have sigma Aldrich dry reagents and have been making the reagents and following the instructions correctly and I did get the stain to work wonderfully when I first started. Then suddenly it quit working and I cannot get it to work again. I have made fresh reagents day after day and I have a pH of 3.8 for the acidulated water. The tissue will stain normally with the yellow to brown background and I the developing solution turns black when it is left on paper but the h.pylori organisms are not staining. I am getting very frustrated and need some help please. Thank you. Tasha Campbell, HTL tmcampbell@fmh.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From ncosenza <@t> siumed.edu Wed Jan 15 08:56:53 2014 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Wed Jan 15 08:57:02 2014 Subject: [Histonet] FISH technique and NBF as fixative Message-ID: <52D6A1B5.6060702@siumed.edu> I need to do FISH with the universal bacterial probe EUB338. I am not too familiar with FISH. Can we fix the sample with NBF or is 4% paraformaldehyde better? The samples are acellular dermal matrix. A quick lit search shows people using 4% paraformaldehyde, but the histologist that will be embedding the tissue said that NBF is gentler on tissue than 4% PFA... From DKnutson <@t> primecare.org Wed Jan 15 10:46:01 2014 From: DKnutson <@t> primecare.org (Knutson, Deanne) Date: Wed Jan 15 10:46:13 2014 Subject: [Histonet] HER2/NEU Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A35E0EDD37@EXCHANGE2K7.staprimecare.org> I just wanted to see what others in the histology world might be experiencing. We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. The clone we are using is CB11 from Cell Marque. We did a HER2 study last year where we sent out our cases for a second opinion, and the study showed others interpreted our Indeterminates as that also. Has anyone else also noted this increase in their annual comparison, and what clone are you using? It is so nice to be able to reach out to our peers with these types of questions. Thank you for responding back! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From mward <@t> wakehealth.edu Wed Jan 15 11:04:10 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Wed Jan 15 11:04:19 2014 Subject: [Histonet] RE: HER2/NEU In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A35E0EDD37@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A35E0EDD37@EXCHANGE2K7.staprimecare.org> Message-ID: That is an interesting observation, as we have seen something of the same thing and we use the Dako Herceptest. I would be interested in hearing others responses as well. Thank you for asking the question. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne Sent: Wednesday, January 15, 2014 11:46 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] HER2/NEU I just wanted to see what others in the histology world might be experiencing. We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. The clone we are using is CB11 from Cell Marque. We did a HER2 study last year where we sent out our cases for a second opinion, and the study showed others interpreted our Indeterminates as that also. Has anyone else also noted this increase in their annual comparison, and what clone are you using? It is so nice to be able to reach out to our peers with these types of questions. Thank you for responding back! Deanne Knutson Anatomic Pathology Supervisor St. Alexius Medical Center 701-530-6730 dknutson@primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Jan 15 12:04:10 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 15 12:04:14 2014 Subject: [Histonet] RE: HER2/NEU In-Reply-To: References: <1E0E2B14C709174B8AC2BE0AE7F76833A35E0EDD37@EXCHANGE2K7.staprimecare.org>, Message-ID: Consider the ASCO-CAP guideline and scoring changes for Her2/ FISH, not sure of the timeframe for your trend in increased positives, but my pathologist has indicated that I should expect more positive and equivocal cases, so perhaps related to categorizing of DX, rather performance or clone related.? see if that correlates Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mward@wakehealth.edu > To: DKnutson@primecare.org; histonet@lists.utsouthwestern.edu > Date: Wed, 15 Jan 2014 17:04:10 +0000 > CC: > Subject: [Histonet] RE: HER2/NEU > > That is an interesting observation, as we have seen something of the same thing and we use the Dako Herceptest. I would be interested in hearing others responses as well. Thank you for asking the question. > > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 > p 336.716.2109 \ f 336.716.5890 > mward@wakehealth.edu > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Knutson, Deanne > Sent: Wednesday, January 15, 2014 11:46 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] HER2/NEU > > I just wanted to see what others in the histology world might be experiencing. > > We have seen a marked increase in the percentage of HER2 cases resulting as Indeterminate, when we compare our last year patient predictive marker results to this year's results. > The clone we are using is CB11 from Cell Marque. > > We did a HER2 study last year where we sent out our cases for a second opinion, and the study showed others interpreted our Indeterminates as that also. > Has anyone else also noted this increase in their annual comparison, and what clone are you using? > > It is so nice to be able to reach out to our peers with these types of questions. > Thank you for responding back! > > Deanne Knutson > Anatomic Pathology Supervisor > St. Alexius Medical Center > 701-530-6730 > dknutson@primecare.org > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nguy0515 <@t> gmail.com Wed Jan 15 12:23:38 2014 From: nguy0515 <@t> gmail.com (Trini) Date: Wed Jan 15 12:23:45 2014 Subject: [Histonet] RE: Recuts/deepers Message-ID: This is my first time working in a GLP/GMP lab, Ive only been here for 6 months. We cut a variety of tissue. At trimming is where they decide what tissue to go in what cassette and so one cassete could have 4-6 different types of tissue. And on the other hand we do osteoinduction studies, and when we get recuts for the OI it means that our first section given to the pathologist did not pass the requirement. The pathologist sometimes list what they are looking for and I have noticed that the problem is the tissue was not embedded at the same plane in the block. I do admit I have cut shallow before because I did not want to go too deep to get the other section and lose the tissue before it (example: adrenals). My plan after I get opinions/advice on histonet is to go talk to the pathologists and study directors. Thank you for your help!!! Trini From TanyaAbbott <@t> catholichealth.net Wed Jan 15 12:35:50 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Jan 15 12:35:54 2014 Subject: [Histonet] Cryostat Decontamination Message-ID: <852F7D2C14FB464D80E182B15DB138AF1FE72D5B@CHIEX005.CHI.catholichealth.net> I am looking for a procedure and product to use for decontamination after a potential TB case, help please! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From SHargrove <@t> unitedregional.org Wed Jan 15 13:02:46 2014 From: SHargrove <@t> unitedregional.org (Susie Hargrove) Date: Wed Jan 15 13:02:51 2014 Subject: [Histonet] 2014 CPT Changes Message-ID: Hello, I know this subject had been looked at many times. I just want to see if I am correct before I start doing anything different. 1. 88342 , not much change . We can still just bill once per site, per case. This is per single antibody per Specimen. If I have a case with two parts and each part has 3 immuno's ordered I will bill them all 88342, Even if I do it on multiple blocks of the same specimen, I will still charge once. If one of these antibodies is a cocktail, I will bill 88342 for the first and 88343 for subsequent parts of that cocktail, and I can do this per specimen. So the only change I see here is the breakdown of the cocktail charges into 88343. 2. Medicare codes, GO461 is ordered for the first antibody , per specimen site per case, all additional antibodies, including cocktails , if any, are billed GO462. As before only 1 antibody can be billed by site/source. Now just to work out how the billing will flip over once the charges go across. Is anybody doing it differently? ________________________________ Susie Hargrove HT (ASCP) Histology Technical Specialist United Regional Health Care Wichita Falls, Texas 76301 Ph 940-764-3881 Fax-940-764-3129 ________________________________ CONFIDENTIALITY NOTICE: This e-mail, and any attachment, may contain identifiable health information that is subject to protection under state and federal law. This information is intended only for the person or entity to which it is addressed. If you are not the intended recipient, be aware that any review, re-transmission, copying, dissemination or other use of this information by persons or entities other than the intended recipient is prohibited and may be punishable by law. If you received this in error, please return it to the sender by electronic mail (reply) and delete the material from any computer or server on which it resides due to your receipt. From TanyaAbbott <@t> catholichealth.net Wed Jan 15 13:51:13 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Jan 15 13:51:18 2014 Subject: [Histonet] Cryostat Message-ID: <852F7D2C14FB464D80E182B15DB138AF1FE72E13@CHIEX005.CHI.catholichealth.net> Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From abtdhu <@t> gmail.com Wed Jan 15 22:02:18 2014 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Wed Jan 15 22:02:22 2014 Subject: [Histonet] Do I have to destabilize MMA? Message-ID: <275D41A2-62A7-4B73-A627-A2A9DE530BF4@gmail.com> I am new to MMA plastic bone technique. Some one gave me his protocol, in which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. But others told me I don't need to do the destabilization step. Could any expert in this area to tell me if this step is necessary? And why have to do? Sent from my iPad From ratliffjack <@t> hotmail.com Thu Jan 16 04:50:21 2014 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Thu Jan 16 04:54:15 2014 Subject: [Histonet] Do I have to destabilize MMA? In-Reply-To: <275D41A2-62A7-4B73-A627-A2A9DE530BF4@gmail.com> References: <275D41A2-62A7-4B73-A627-A2A9DE530BF4@gmail.com> Message-ID: Dorothy, There are some that completely believe that it is necessary to destabilize MMA prior to use and they are not necessarily wrong as the protocol has worked for them without issue......so I assume. I personally have NEVER had to or tried this destabilization method, quite frankly because I have NEVER had a problem creating an acrylic resin embedded block, regardless of the tissue or size of the tissue, when simply combining monomer (MMA) with softener (DBP) and catalyst (Perkadox 16) in my almost 15 years of exclusively working with this resin for demonstrating bone, biomaterials and medical device implants. Again, I am not saying that the protocol given to you does not work, but rather it is my personal experience that this destabilizing method is an unnecessary step and a waste of time and expense. Hope this helps and please feel free to contact me if you have any additional questions or concerns. Best Regards, Jack Ratliff > On Jan 15, 2014, at 10:02 PM, abtdhu@gmail.com wrote: > > I am new to MMA plastic bone technique. Some one gave me his protocol, in which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. But others told me I don't need to do the destabilization step. Could any expert in this area to tell me if this step is necessary? And why have to do? > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Thu Jan 16 07:32:39 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jan 16 07:32:46 2014 Subject: [Histonet] RE: error rate for sectioning In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D2964BCE8@AHCMSASEXCH03.my.ahc.local> References: <997D36BD8D0ACF418AF900F4E781DE4414BCB4D6@MLBXv03.nih.gov>, <4A2A16B9707CE04E9CB6C82DC18C1D2964BCE8@AHCMSASEXCH03.my.ahc.local> Message-ID: My current QA monitors Not more than 0.17% slides mislabeledNot more than 1.0% of tissue blocks-reoriented ( embedding errors)Not more than 1.0% of tissue blocks reprocessed ( gross problem)Not more than 1% of specials and IHC stain slides repeated ( technical issues-not DX)Not more than 0.1% of slides, with documented evidence of cross contamination, requires RCA Based mostly on CAP- HQUIP, but some literature. Use LIS and HC1 to track in addition to manual methods for reporting, repeats etc. The medical director (pathologist) supervises , provides oversight for the quality issues and resulting corrective actions. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Michael.LaFriniere@ccplab.com > To: zerfasp@ors.od.nih.gov; histonet@lists.utsouthwestern.edu > Date: Tue, 14 Jan 2014 19:32:56 +0000 > CC: > Subject: [Histonet] RE: error rate for sectioning > > Hi Patricia, > > Error rates should not be set by employers. You should have an effective QA management program and committee even in small practices with the Medical Director as the Chair. The committee sets and establishes error rate thresholds with monthly monitors in place to seek opportunities of improvement should error rates exceed below the set threshold. > > Hope this helps! > > Michael > > Michael R. LaFriniere, HT (ASCP) > Executive Director > > > Capital Choice Pathology Laboratory > > 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 > > P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 > > michael.lafriniere@CCPLab.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zerfas, Patricia (NIH/OD/ORS) [E] > Sent: Saturday, January 11, 2014 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] error rate for sectioning > > > > Hi All, > What is the error rate set by you or your employer for paraffin sectioning? > > These are slides that are found to be unacceptable due to wrinkling, folding etc either by a pathologist or another reviewer. > > I was found to have 6 slides out of approx 1500 that I have sectioned to date to be unacceptable. I only perform this duty when I have completed all of my other work so this is not one of my regular duties. At times I have gone several months without sectioning and utilize a microtome that is approx. 26 years old. > > Since this was brought to my attention I have been given more up to date equipment which I have yet to utilize. > > Thanks in advance for your feedback. > > Patricia Zerfas > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abtdhu <@t> gmail.com Thu Jan 16 08:01:47 2014 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Thu Jan 16 08:01:57 2014 Subject: [Histonet] Re: Do I have to destabilize MMA? In-Reply-To: <275D41A2-62A7-4B73-A627-A2A9DE530BF4@gmail.com> References: <275D41A2-62A7-4B73-A627-A2A9DE530BF4@gmail.com> Message-ID: Thanks Jack's answer. I though I shouldn't to destabilization step as well. But my boss wants to do it. Is there a chemical theory behind this? Why previous protocol has this step (it must be reason)? And why the step is skipped now? I wish I know before approaching my boss. Additionally, there is a problem always bothering me in plastic sectioning. I often get cortical bone shattering ( crumbly) in the midshaft of long bone. Cortical bone and marrow are operate in the midshaft. I don't know why is that. It's not happen in the two ends of tibia and femur bone. And doesn't happen on ankle bones. Is that because the bone was stored in 70% EtOH too long (~one year)? I think infiltration is good since I did three times, 2 days each infiltration under vacuum condition in 4oC. If I use 40% EtOH to fix and store the bones will help? One more question. What is advantage if I use perkadox 16 to replace BP as catalyst? Do I have to dry MMA//DBP/perkadox 16 mixture through CaCl2? Thanks in advance for your help. Dorothy MGH endocrine histocore On Wed, Jan 15, 2014 at 11:02 PM, wrote: > I am new to MMA plastic bone technique. Some one gave me his protocol, in > which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. > But others told me I don't need to do the destabilization step. Could any > expert in this area to tell me if this step is necessary? And why have to > do? > > Sent from my iPad From jkiernan <@t> uwo.ca Thu Jan 16 12:37:59 2014 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Jan 16 12:38:04 2014 Subject: [Histonet] Re: Do I have to destabilize MMA? In-Reply-To: <7440e58b44139.52d826f8@uwo.ca> References: <275D41A2-62A7-4B73-A627-A2A9DE530BF4@gmail.com> <7330ef0a45261.52d81ed0@uwo.ca> <7340d95a44ef4.52d81f0d@uwo.ca> <73f0fe6e46713.52d81f4b@uwo.ca> <73a09438420e7.52d81f89@uwo.ca> <73a0883647467.52d81fc8@uwo.ca> <73f0b93646521.52d82006@uwo.ca> <73b0887a40207.52d82135@uwo.ca> <73e0ab1646ea9.52d82173@uwo.ca> <72f0bfd9472c5.52d821b1@uwo.ca> <73f0bff243634.52d821f0@uwo.ca> <73f0e1ed40886.52d82396@uwo.ca> <73b0c24b47d34.52d823d4@uwo.ca> <73e0bcca47450.52d82413@uwo.ca> <73a0a0464207c.52d82451@uwo.ca> <73d0bd2940016.52d8248f@uwo.ca> <72f08f1547cd6.52d824ce@uwo.ca> <740091684733c.52d8250c@uwo.ca> <73a0a0e645f39.52d8254c@uwo.ca> <73f0b478425f4.52d8267b@uwo.ca> <7350a070473b8.52d826b9@uwo.ca> <7440e58b44139.52d826f8@uwo.ca> Message-ID: <7340d0e843f89.52d7e0b7@uwo.ca> Methacrylate monomers are sold with added hydroquinone to inhibit spontaneous polymerization. The NaOH treatment to remove hydroquinone dates from the earliest years of plastic embedding (Newman et al. 1949 Science 110: 66-68). The NaOH treatment is not necessary, however, because the stabilizing action of hydroquinone can be overcome by simply using a larger amount of the polymerization catalyst. About 2% of either benzoyl peroxide or 1,2-dichlorobenzoyl peroxide will catalyze the polymerization of stabilized methacrylate monomer. See Hayat, MA 1981 Principles and Techniques of Electron Microscopy Vol 1. Baltimore: University Park Press, pp. 167-168. See Baker, JR 1960 Cytological Technique, 4th ed. London: Methuen, pp.77-84 for a thorough but simple explanation of the chemistry of methacrylate embedding. The whole book (which is a classic) is available, free, at http://archive.org. John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = On 16/01/14, Dorothy Hu wrote: > Thanks Jack's answer. I though I shouldn't to destabilization step as well. > But my boss wants to do it. Is there a chemical theory behind this? Why > previous protocol has this step (it must be reason)? And why the step is > skipped now? I wish I know before approaching my boss. > > Additionally, there is a problem always bothering me in plastic sectioning. > I often get cortical bone shattering ( crumbly) in the midshaft of long > bone. Cortical bone and marrow are operate in the midshaft. I don't know > why is that. It's not happen in the two ends of tibia and femur bone. And > doesn't happen on ankle bones. Is that because the bone was stored in 70% > EtOH too long (~one year)? I think infiltration is good since I did three > times, 2 days each infiltration under vacuum condition in 4oC. If I use 40% > EtOH to fix and store the bones will help? > > One more question. What is advantage if I use perkadox 16 to replace BP as > catalyst? Do I have to dry MMA//DBP/perkadox 16 mixture through CaCl2? > > Thanks in advance for your help. > > Dorothy > MGH endocrine histocore > > > On Wed, Jan 15, 2014 at 11:02 PM, wrote: > > > I am new to MMA plastic bone technique. Some one gave me his protocol, in > > which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. > > But others told me I don't need to do the destabilization step. Could any > > expert in this area to tell me if this step is necessary? And why have to > > do? > > > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From madelinegi <@t> yahoo.com Thu Jan 16 12:43:09 2014 From: madelinegi <@t> yahoo.com (Madeline Gi) Date: Thu Jan 16 12:43:17 2014 Subject: [Histonet] Cryostat In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF1FE72E13@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF1FE72E13@CHIEX005.CHI.catholichealth.net> Message-ID: <1389897789.16602.YahooMailNeo@web121504.mail.ne1.yahoo.com> I use to decon my cryostat with 100% Alco this keeps it from freezing up and it clean it.? best of luck.? ? Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com On Wednesday, January 15, 2014 2:52 PM, "Abbott, Tanya" wrote: Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph? 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From egray <@t> hsc.wvu.edu Thu Jan 16 14:11:01 2014 From: egray <@t> hsc.wvu.edu (Gray, Ed) Date: Thu Jan 16 14:11:16 2014 Subject: [Histonet] 2014 CPT Changes Message-ID: <2acca10d7e7d40458bd972de9c2491e4@BN1PR05MB406.namprd05.prod.outlook.com> So elegant in its simplicity, I think I'll borrow it. Looks right to me. Ed Gray | Clinical Application Coordinator | Phone: 304-293-2945 | Fax: 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Message: 4 Date: Wed, 15 Jan 2014 19:02:46 +0000 From: Susie Hargrove Subject: [Histonet] 2014 CPT Changes To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, I know this subject had been looked at many times. I just want to see if I am correct before I start doing anything different. 1. 88342 , not much change . We can still just bill once per site, per case. This is per single antibody per Specimen. If I have a case with two parts and each part has 3 immuno's ordered I will bill them all 88342, Even if I do it on multiple blocks of the same specimen, I will still charge once. If one of these antibodies is a cocktail, I will bill 88342 for the first and 88343 for subsequent parts of that cocktail, and I can do this per specimen. So the only change I see here is the breakdown of the cocktail charges into 88343. 2. Medicare codes, GO461 is ordered for the first antibody , per specimen site per case, all additional antibodies, including cocktails , if any, are billed GO462. As before only 1 antibody can be billed by site/source. Now just to work out how the billing will flip over once the charges go across. Is anybody doing it differently? ________________________________ Susie Hargrove HT (ASCP) Histology Technical Specialist United Regional Health Care Wichita Falls, Texas 76301 Ph 940-764-3881 Fax-940-764-3129 ________________________________ CONFIDENTIALITY NOTICE: This e-mail, and any attachment, may contain identifiable health information that is subject to protection under state and federal law. This information is intended only for the person or entity to which it is addressed. If you are not the intended recipient, be aware that any review, re-transmission, copying, dissemination or other use of this information by persons or entities other than the intended recipient is prohibited and may be punishable by law. If you received this in error, please return it to the sender by electronic mail (reply) and delete the material from any computer or server on which it resides due to your receipt. ------------------------------ Message: 5 Date: Wed, 15 Jan 2014 19:51:13 +0000 From: "Abbott, Tanya" Subject: [Histonet] Cryostat To: "Histonet@lists.utsouthwestern.edu" Message-ID: <852F7D2C14FB464D80E182B15DB138AF1FE72E13@CHIEX005.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 6 Date: Wed, 15 Jan 2014 23:02:18 -0500 From: abtdhu@gmail.com Subject: [Histonet] Do I have to destabilize MMA? To: "histonet@lists.utsouthwestern.edu" Message-ID: <275D41A2-62A7-4B73-A627-A2A9DE530BF4@gmail.com> Content-Type: text/plain; charset=us-ascii I am new to MMA plastic bone technique. Some one gave me his protocol, in which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. But others told me I don't need to do the destabilization step. Could any expert in this area to tell me if this step is necessary? And why have to do? Sent from my iPad ------------------------------ Message: 7 Date: Thu, 16 Jan 2014 04:50:21 -0600 From: Jack Ratliff Subject: Re: [Histonet] Do I have to destabilize MMA? To: "abtdhu@gmail.com" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Dorothy, There are some that completely believe that it is necessary to destabilize MMA prior to use and they are not necessarily wrong as the protocol has worked for them without issue......so I assume. I personally have NEVER had to or tried this destabilization method, quite frankly because I have NEVER had a problem creating an acrylic resin embedded block, regardless of the tissue or size of the tissue, when simply combining monomer (MMA) with softener (DBP) and catalyst (Perkadox 16) in my almost 15 years of exclusively working with this resin for demonstrating bone, biomaterials and medical device implants. Again, I am not saying that the protocol given to you does not work, but rather it is my personal experience that this destabilizing method is an unnecessary step and a waste of time and expense. Hope this helps and please feel free to contact me if you have any additional questions or concerns. Best Regards, Jack Ratliff > On Jan 15, 2014, at 10:02 PM, abtdhu@gmail.com wrote: > > I am new to MMA plastic bone technique. Some one gave me his protocol, in which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. But others told me I don't need to do the destabilization step. Could any expert in this area to tell me if this step is necessary? And why have to do? > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Thu, 16 Jan 2014 13:32:39 +0000 From: joelle weaver Subject: RE: [Histonet] RE: error rate for sectioning To: Michael LaFriniere , "Zerfas, Patricia (NIHODORS) [E]" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" My current QA monitors Not more than 0.17% slides mislabeledNot more than 1.0% of tissue blocks-reoriented ( embedding errors)Not more than 1.0% of tissue blocks reprocessed ( gross problem)Not more than 1% of specials and IHC stain slides repeated ( technical issues-not DX)Not more than 0.1% of slides, with documented evidence of cross contamination, requires RCA Based mostly on CAP- HQUIP, but some literature. Use LIS and HC1 to track in addition to manual methods for reporting, repeats etc. The medical director (pathologist) supervises , provides oversight for the quality issues and resulting corrective actions. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Michael.LaFriniere@ccplab.com > To: zerfasp@ors.od.nih.gov; histonet@lists.utsouthwestern.edu > Date: Tue, 14 Jan 2014 19:32:56 +0000 > CC: > Subject: [Histonet] RE: error rate for sectioning > > Hi Patricia, > > Error rates should not be set by employers. You should have an effective QA management program and committee even in small practices with the Medical Director as the Chair. The committee sets and establishes error rate thresholds with monthly monitors in place to seek opportunities of improvement should error rates exceed below the set threshold. > > Hope this helps! > > Michael > > Michael R. LaFriniere, HT (ASCP) > Executive Director > > > Capital Choice Pathology Laboratory > > 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 > > P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 > > michael.lafriniere@CCPLab.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Zerfas, Patricia (NIH/OD/ORS) [E] > Sent: Saturday, January 11, 2014 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] error rate for sectioning > > > > Hi All, > What is the error rate set by you or your employer for paraffin sectioning? > > These are slides that are found to be unacceptable due to wrinkling, folding etc either by a pathologist or another reviewer. > > I was found to have 6 slides out of approx 1500 that I have sectioned to date to be unacceptable. I only perform this duty when I have completed all of my other work so this is not one of my regular duties. At times I have gone several months without sectioning and utilize a microtome that is approx. 26 years old. > > Since this was brought to my attention I have been given more up to date equipment which I have yet to utilize. > > Thanks in advance for your feedback. > > Patricia Zerfas > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 16 Jan 2014 09:01:47 -0500 From: Dorothy Hu Subject: [Histonet] Re: Do I have to destabilize MMA? To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thanks Jack's answer. I though I shouldn't to destabilization step as well. But my boss wants to do it. Is there a chemical theory behind this? Why previous protocol has this step (it must be reason)? And why the step is skipped now? I wish I know before approaching my boss. Additionally, there is a problem always bothering me in plastic sectioning. I often get cortical bone shattering ( crumbly) in the midshaft of long bone. Cortical bone and marrow are operate in the midshaft. I don't know why is that. It's not happen in the two ends of tibia and femur bone. And doesn't happen on ankle bones. Is that because the bone was stored in 70% EtOH too long (~one year)? I think infiltration is good since I did three times, 2 days each infiltration under vacuum condition in 4oC. If I use 40% EtOH to fix and store the bones will help? One more question. What is advantage if I use perkadox 16 to replace BP as catalyst? Do I have to dry MMA//DBP/perkadox 16 mixture through CaCl2? Thanks in advance for your help. Dorothy MGH endocrine histocore On Wed, Jan 15, 2014 at 11:02 PM, wrote: > I am new to MMA plastic bone technique. Some one gave me his protocol, > in which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. > But others told me I don't need to do the destabilization step. Could > any expert in this area to tell me if this step is necessary? And why > have to do? > > Sent from my iPad ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 122, Issue 19 ***************************************** From mucram11 <@t> comcast.net Thu Jan 16 15:40:53 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Jan 16 15:41:15 2014 Subject: [Histonet] Arkansas Society for Histotechnology Spring Meeting in Hot Springs AR Message-ID: <927752425.163543.1389908453034.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> The Arkansas Society for Histotechnolgy is holding it's 40th Anniversay Meeting in Hot Springs, AR ? The program is listed and if you wish a registration packet with full program/abstracts and information please send an email and we will?be happy to send it and update you for your trip to Hot Springs.? The e-mail can be replied to through HistoNet or to ashnews@comcast.net ?? ? Please note we are having a full HT Registry Course on Saturday March 1st with Shane Jones!! February 28th FRIDAY MORNING WORKSHOPS 8:00AM to 11:30AM IHC from the Beginning ??????????????? Bonnie Whitaker, Anatomic Pathology Operations Director Speaker: To Be Announced. ? Speaker withdrew We will update ASAP!? 90 Minute Talk Integrating Mobile Technology With Anatomic Pathology?????????? 90 Minute Talk Dr Shree Sharma Pathology Department of UAMS ?? February 28th AFTERNOON WORKSHOPS 1:00PM to 4:30PM ? I Have No Idea What I Am Looking At!! ? (Tissue Identification) Shane Jones BS, HT (ASCP) School of Histotechnology Program Director ? What?s New in HER2 Treatment, Testing and Regulation? Lynn Charpentier, Marketing Product Manager ? March 1 st - SATURDAY MORNING 8:00AM TO 11:30AM ? Hazard Communication Standard/GHS Awareness and Formaldehyde Training Part One: ? 90 MINUTES These are new regulations for waste handling today in Part 1 and Part 2 Part Two: 90 MINUTES ? Basic Microtomy Mari Ann Mailhiot, Technical Specialist ? MARCH 1 - SATURDAY AFTERNOON 1:00PM TO 4:30PM ? Basic IHC and Troubleshooting Workshop Matthew Pardilla, Technical Consultant ? Unraveling the Mysteries of Histotechnology ? Debbie Siena Histology Product Manger ??????????????? ? MARCH 1ST SATURDAY ? CLASS BEGINS AT 8:00AM AND ENDS AT 4:30PM SPECIAL CLASS ALL FOR HISTOLOGY REGISTRY COURSE I HAVE TO KNOW ALL OF THAT FOR THE EXAM??? ?????????? Shane Jones BS, HT (ASCP) From tbraud <@t> holyredeemer.com Thu Jan 16 15:51:00 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 16 15:51:06 2014 Subject: [Histonet] 2014 billing changes In-Reply-To: <20140116172652.9948F1E8068@trendmess-svr.holyredeemer.local> References: <20140116172652.9948F1E8068@trendmess-svr.holyredeemer.local> Message-ID: Hi Susie and fellow Histonetters : Susie - I am hoping to bill as you describe. To clarify - I will only use the 88343 when billing additional antibodies in a cocktails that can be separately visualized on one slide, identified with different chromogens, such as PIN-4. I will not use it for antibody cocktails, such as AE1/AE3, both stained with DAB. How I'm going to have our LIS translate the billing for CMS patients is another store. I'm having a similar issue with the changes in prostate biopsy billing for CMS patients. Arrrrrrggggghhhhhh! Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 4. 2014 CPT Changes (Susie Hargrove) Message: 4 Date: Wed, 15 Jan 2014 19:02:46 +0000 From: Susie Hargrove Subject: [Histonet] 2014 CPT Changes Hello, I know this subject had been looked at many times. I just want to see if I am correct before I start doing anything different. 1. 88342 , not much change . We can still just bill once per site, per case. This is per single antibody per Specimen. If I have a case with two parts and each part has 3 immuno's ordered I will bill them all 88342, Even if I do it on multiple blocks of the same specimen, I will still charge once. If one of these antibodies is a cocktail, I will bill 88342 for the first and 88343 for subsequent parts of that cocktail, and I can do this per specimen. So the only change I see here is the breakdown of the cocktail charges into 88343. 2. Medicare codes, GO461 is ordered for the first antibody , per specimen site per case, all additional antibodies, including cocktails , if any, are billed GO462. As before only 1 antibody can be billed by site/source. Now just to work out how the billing will flip over once the charges go across. Is anybody doing it differently? Susie Hargrove HT (ASCP) Histology Technical Specialist United Regional Health Care Wichita Falls, Texas 76301 Ph 940-764-3881 Fax-940-764-3129 ************************************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From cmconway <@t> usgs.gov Thu Jan 16 15:51:56 2014 From: cmconway <@t> usgs.gov (Conway, Carla) Date: Thu Jan 16 15:52:12 2014 Subject: [Histonet] Leica TP1020 tissue processor comments? Message-ID: Hello, Our lab is considering purchasing a new tissue processor. We have an IMS LX-120 benchtop processor and would like another benchtop model, if possible. I'd appreciate any comments you may have regarding the Leica TP1020 processor. Thanks very much! Carla **Please note new phone number** Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov From modz9636 <@t> gmail.com Thu Jan 16 19:30:20 2014 From: modz9636 <@t> gmail.com (M.O.) Date: Thu Jan 16 19:30:25 2014 Subject: [Histonet] Human bone and soft tissue processing issues Message-ID: Hello Histonet! I am hoping that someone could shed some light on some issues I am having with some human knee tissues embedded in paraffin. About one year ago, we started having some issues with the established protocol and decided to increase the processing times. Issues we are seeing: Once processed, the stained ligaments (MCL, LCL, ACL, etc) appear to have brittle tissue (sort of wavy looking) toward the middle of the sample in stained sections. The block itself has white brittle areas that flake off. Regarding bone slabs, we find that the junction between the cartilage and bone is where the tissues are the hardest after decal and the color is different in this area. We think that the fixation may be a problem because of penetration. If you have any suggestions for ensuring complete fixation, that would be very helpful! The processor run does not include a fixative station. Soft Tissues and Ligaments: All tissues are trimmed down to about 4mm in thickness and put into Anatech?s Z-fix for 5 days with one change of Z-fix. Washed and put into 70% EtOH. Bone: Slabs are 6-9mm thick (we ideally aim for 7mm) across the tibial plateau and along the medial and lateral femoral condyles ? with cartilage and about 1cm of subchondral bone. The slabs are individually fixed with Z-fix for 1 week, with one change of Z-fix and excess bone trimmed after the first 3 days. Then the samples are put into TBD-2 (formic acid decal) for up to 2 weeks depending on the end point, with a change of decal. Once decalcified, the samples are cut into 20mm X 4mm X 7mm pieces and washed and put into 70% EtOH. The processor completes all steps under vacuum. Older Protocol with another processor: 70%, 80%, 2X95%, 3X100%, 3XPropar, 3XParaffin All reagents are 2 hours. Old Protocol with Thermo Scientific Excelsior Processor: 70%, 80%, 2X95%, 3X100%, 3XPropar, 3XParaffin All reagents are 4 hours. Recent Protocol (past year) Thermo Scientific Excelsior Processor: 70% 1hr 85% - 4hrs 95% - 4hrs 2X100% - 4hrs 100% - 5hrs 2XPropar - 4hrs Propar - 5hrs 2XParaffin - 4hrs Paraffin - 5hrs The main questions are: 1) How can we ensure complete fixation? 2) What is causing these brittle/hard areas in our tissues, even for soft tissues? 3) What does over-processing look like in samples? 4) Is our recent protocol okay or does it need adjustments? Thank you in advance for any help or suggestions! Sincerely, Merissa From abtdhu <@t> gmail.com Thu Jan 16 19:47:36 2014 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Thu Jan 16 19:47:39 2014 Subject: [Histonet] Re: Do I have to destabilize MMA? In-Reply-To: <7340d0e843f89.52d7e0b7@uwo.ca> References: <275D41A2-62A7-4B73-A627-A2A9DE530BF4@gmail.com> <7330ef0a45261.52d81ed0@uwo.ca> <7340d95a44ef4.52d81f0d@uwo.ca> <73f0fe6e46713.52d81f4b@uwo.ca> <73a09438420e7.52d81f89@uwo.ca> <73a0883647467.52d81fc8@uwo.ca> <73f0b93646521.52d82006@uwo.ca> <73b0887a40207.52d82135@uwo.ca> <73e0ab1646ea9.52d82173@uwo.ca> <72f0bfd9472c5.52d821b1@uwo.ca> <73f0bff243634.52d821f0@uwo.ca> <73f0e1ed40886.52d82396@uwo.ca> <73b0c24b47d34.52d823d4@uwo.ca> <73e0bcca47450.52d82413@uwo.ca> <73a0a0464207c.52d82451@uwo.ca> <73d0bd2940016.52d8248f@uwo.ca> <72f08f1547cd6.52d824ce@uwo.ca> <740091684733c.52d8250c@uwo.ca> <73a0a0e645f39.52d8254c@uwo.ca> <73f0b478425f4.52d8267b@uwo.ca> <7350a070473b8.52d826b9@uwo.ca> <7440e58b44139.52d826f8@uwo.ca> <7340d0e843f89.52d7e0b7@uwo.ca> Message-ID: Thank John very much. I will check out your references. Hope we are not only be able to follow the protocol, but fully understand it. Dorothy Sent from my iPad > On Jan 16, 2014, at 1:37 PM, John Kiernan wrote: > > Methacrylate monomers are sold with added hydroquinone to inhibit spontaneous polymerization. The NaOH treatment to remove hydroquinone dates from the earliest years of plastic embedding (Newman et al. 1949 Science 110: 66-68). The NaOH treatment is not necessary, however, because the stabilizing action of hydroquinone can be overcome by simply using a larger amount of the polymerization catalyst. About 2% of either benzoyl peroxide or 1,2-dichlorobenzoyl peroxide will catalyze the polymerization of stabilized methacrylate monomer. See Hayat, MA 1981 Principles and Techniques of Electron Microscopy Vol 1. Baltimore: University Park Press, pp. 167-168. > > See Baker, JR 1960 Cytological Technique, 4th ed. London: Methuen, pp.77-84 for a thorough but simple explanation of the chemistry of methacrylate embedding. The whole book (which is a classic) is available, free, at http://archive.org. > > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = >> On 16/01/14, Dorothy Hu wrote: >> Thanks Jack's answer. I though I shouldn't to destabilization step as well. >> But my boss wants to do it. Is there a chemical theory behind this? Why >> previous protocol has this step (it must be reason)? And why the step is >> skipped now? I wish I know before approaching my boss. >> >> Additionally, there is a problem always bothering me in plastic sectioning. >> I often get cortical bone shattering ( crumbly) in the midshaft of long >> bone. Cortical bone and marrow are operate in the midshaft. I don't know >> why is that. It's not happen in the two ends of tibia and femur bone. And >> doesn't happen on ankle bones. Is that because the bone was stored in 70% >> EtOH too long (~one year)? I think infiltration is good since I did three >> times, 2 days each infiltration under vacuum condition in 4oC. If I use 40% >> EtOH to fix and store the bones will help? >> >> One more question. What is advantage if I use perkadox 16 to replace BP as >> catalyst? Do I have to dry MMA//DBP/perkadox 16 mixture through CaCl2? >> >> Thanks in advance for your help. >> >> Dorothy >> MGH endocrine histocore >> >> >> On Wed, Jan 15, 2014 at 11:02 PM, wrote: >> >> > I am new to MMA plastic bone technique. Some one gave me his protocol, in >> > which has NaOH and d-water to wash MMA mixture before drying it in CaCl2. >> > But others told me I don't need to do the destabilization step. Could any >> > expert in this area to tell me if this step is necessary? And why have to >> > do? >> > >> > Sent from my iPad >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From a.prior <@t> tissueregenix.com Fri Jan 17 05:10:52 2014 From: a.prior <@t> tissueregenix.com (Andrew Prior) Date: Fri Jan 17 05:11:43 2014 Subject: [Histonet] Slide interpretation training Message-ID: Hi, I am looking to improve my slide interpretation skills, particularly in assessing tissue quality and structure in meniscus, skin and arteries (Picrosirius Red and Movat's Pentachrome stained sections). I work for a small company, with no pathologist on the pay roll, so the task has fallen to me. While I have some experience in slide interpretation, I am not as familiar with these tissues as I would like. Does anyone know of a suitable course or a pathologist willing to work as a mentor, preferably based in the UK that could provide training in these areas? Many thanks Andrew Andrew Prior Histologist Tissue Regenix Group Heslington, York, UK YO10 5NY E-mail: a.prior@tissueregenix.com Website: www.tissueregenix.com The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Although we routinely screen for viruses, addressees should check this e-mail and any attachment for viruses. We make no warranty as to absence of viruses in this e-mail or any attachments. Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 5NY Registered No. 05969271 From tmcampbell <@t> fmh.org Fri Jan 17 07:43:04 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Fri Jan 17 07:43:14 2014 Subject: [Histonet] Microwave processing Message-ID: <3566D9E34287BE4B95372179009446A0193565EC@EXCHANGE.fmhnt.fmh.org> Since I have been microwave processing GI biopsies, I have noticed that there is a blurry, fuzzy artifact on most of my slides in random spots on the tissue when looking under the scope. I asked the pathologist and he said he notices it too but he doesn't know what it is and it doesn't interfere with interpretation but it still bothers me. Does anyone else have this problem? I am almost certain it is from processing. I have stained some slides of tissue that were processed on a normal processor and I do not see that phenomenon. Is this due to microwave processing and is there a fix for it? Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From jstaruk <@t> masshistology.com Fri Jan 17 07:54:56 2014 From: jstaruk <@t> masshistology.com (Mass Histology) Date: Fri Jan 17 07:54:56 2014 Subject: [Histonet] Slide interpretation training In-Reply-To: References: Message-ID: <004901cf138b$b5113760$1f33a620$@masshistology.com> Check out "WashingtonDeceit" on YouTube. This pathologist has over 500 excellent lessons on microscopic histopathology. http://www.youtube.com/user/WashingtonDeceit _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Prior Sent: Friday, January 17, 2014 6:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide interpretation training Hi, I am looking to improve my slide interpretation skills, particularly in assessing tissue quality and structure in meniscus, skin and arteries (Picrosirius Red and Movat's Pentachrome stained sections). I work for a small company, with no pathologist on the pay roll, so the task has fallen to me. While I have some experience in slide interpretation, I am not as familiar with these tissues as I would like. Does anyone know of a suitable course or a pathologist willing to work as a mentor, preferably based in the UK that could provide training in these areas? Many thanks Andrew Andrew Prior Histologist Tissue Regenix Group Heslington, York, UK YO10 5NY E-mail: a.prior@tissueregenix.com Website: www.tissueregenix.com The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Although we routinely screen for viruses, addressees should check this e-mail and any attachment for viruses. We make no warranty as to absence of viruses in this e-mail or any attachments. Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 5NY Registered No. 05969271 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4259 / Virus Database: 3681/7009 - Release Date: 01/16/14 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4259 / Virus Database: 3681/7007 - Release Date: 01/16/14 From HornHV <@t> archildrens.org Fri Jan 17 08:10:10 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jan 17 08:10:15 2014 Subject: [Histonet] air exchanges Message-ID: <25A4DE08332B19499904459F00AAACB719DE6DEBC7@EVS1.archildrens.org> Are there a set number of air exchanges for a histology lab? References? Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From abtdhu <@t> gmail.com Fri Jan 17 08:56:38 2014 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Fri Jan 17 08:56:43 2014 Subject: [Histonet] Help on get PDF file paper from J. Histotechnology Message-ID: Hi Histonetters, Would you please let me know why I can not get PDF file from J Histotechnology any more? I renewed my membership after new year and changed my password since i don't remember my old one. I can get in Maney Online for only Abstract, but not whole article. The paper I tried to get is: Parallel experience of two different lab with initiator perkadox 16 for polymerization of MMA. Vol 17, No 4, pp.343-348. Thanks in advance. Dorothy From joelleweaver <@t> hotmail.com Fri Jan 17 09:38:20 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Jan 17 09:38:25 2014 Subject: [Histonet] Slide interpretation training In-Reply-To: <004901cf138b$b5113760$1f33a620$@masshistology.com> References: , <004901cf138b$b5113760$1f33a620$@masshistology.com> Message-ID: Yes these are excellent for learning gross and microscopic tissue id- I also highly recommend Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jstaruk@masshistology.com > To: a.prior@tissueregenix.com; histonet@lists.utsouthwestern.edu > Date: Fri, 17 Jan 2014 08:54:56 -0500 > Subject: RE: [Histonet] Slide interpretation training > CC: > > Check out "WashingtonDeceit" on YouTube. This pathologist has over 500 > excellent lessons on microscopic histopathology. > > http://www.youtube.com/user/WashingtonDeceit > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrew Prior > Sent: Friday, January 17, 2014 6:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Slide interpretation training > > Hi, > I am looking to improve my slide interpretation skills, particularly in > assessing tissue quality and structure in meniscus, skin and arteries > (Picrosirius Red and Movat's Pentachrome stained sections). I work for a > small company, with no pathologist on the pay roll, so the task has fallen > to me. While I have some experience in slide interpretation, I am not as > familiar with these tissues as I would like. > Does anyone know of a suitable course or a pathologist willing to work as a > mentor, preferably based in the UK that could provide training in these > areas? > > Many thanks > > Andrew > > Andrew Prior > Histologist > Tissue Regenix Group > Heslington, York, UK > YO10 5NY > E-mail: a.prior@tissueregenix.com > Website: www.tissueregenix.com > > The information transmitted is intended only for the person or entity to > which it is addressed and may contain confidential and/or privileged > material. Any review, retransmission, dissemination or other use of, or > taking of any action in reliance upon, this information by persons or > entities other than the intended recipient is prohibited. If you received > this in error, please contact the sender and delete the material from any > computer. Although we routinely screen for viruses, addressees should check > this e-mail and any attachment for viruses. We make no warranty as to > absence of viruses in this e-mail or any attachments. > > Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 5NY > Registered No. 05969271 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2014.0.4259 / Virus Database: 3681/7009 - Release Date: 01/16/14 > > > ----- > No virus found in this message. > Checked by AVG - www.avg.com > Version: 2014.0.4259 / Virus Database: 3681/7007 - Release Date: 01/16/14 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cindy38017 <@t> yahoo.com Fri Jan 17 12:44:07 2014 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Fri Jan 17 12:44:13 2014 Subject: [Histonet] Greetings Histonet Message-ID: http://dimension-x.net/foxnews.php?hfywcdrbz1195fqy Fri, 17 Jan 2014 19:44:07 cindy dewar The only stupid question is the one that is never asked, except maybe "Don't you think it is about time you audited my return?" or "Isn't it morally wrong to give me a warning when, in fact, I was speeding?" From abtdhu <@t> gmail.com Fri Jan 17 14:45:40 2014 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Fri Jan 17 14:45:45 2014 Subject: [Histonet] Help on get PDF file paper from J. Histotechnology In-Reply-To: <16F90B93CA23D446980B3D591FD02DAD0CC01C@UWHC-MBX14.uwhis.hosp.wisc.edu> References: <16F90B93CA23D446980B3D591FD02DAD0CC01C@UWHC-MBX14.uwhis.hosp.wisc.edu> Message-ID: Thanks very much Jean. I will call NSH office. Dorothy On Fri, Jan 17, 2014 at 2:53 PM, Mitchell Jean A wrote: > Dorothy: I know that there have been some issues with Maney Online > lately. I suggest your contact the NSH office and they should be able to > assist you. I had the same problem last week and it was handled very > quickly. > > > Jean Mitchell, BS HT (ASCP) > University of Wisconsin Hospital & Clinics > Neuromuscular Laboratory Manager > 600 Highland Avenue > Madison, WI 53792-5132 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dorothy Hu > Sent: Friday, January 17, 2014 8:57 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Help on get PDF file paper from J. Histotechnology > > Hi Histonetters, > > Would you please let me know why I can not get PDF file from J > Histotechnology any more? > > I renewed my membership after new year and changed my password since i > don't remember my old one. I can get in Maney Online for only Abstract, > but not whole article. > > The paper I tried to get is: > Parallel experience of two different lab with initiator perkadox 16 for > polymerization of MMA. > Vol 17, No 4, pp.343-348. > > Thanks in advance. > > Dorothy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hlukey <@t> msn.com Fri Jan 17 16:07:00 2014 From: hlukey <@t> msn.com (Hugh Luk) Date: Fri Jan 17 16:07:04 2014 Subject: [Histonet] Cryostat Message-ID: Tanya and Madeline, Jan Minshew of Leica sent a nicely detailed response to "Decontaminating" a cryostat in January of 2010. She apparently wrote it to put in a Leica "How To" manual, although I did not notice it in this form. Everything she states is well documented, although I also incorporate a known virucide and tuberculocidal product (following it's directions, naturally). http://lists.utsouthwestern.edu/pipermail/histonet/2010-January/048750.html If you have a UV cryostat, use Leica's Cryofect product or follow Tim Morken's previous (2000 histosearch)formaldehyde method, they should work for killing most bugs too(with the exception of eradicating some prions like CJD/BSE). If I am misunderstanding what you mean by "Decon" I apologize, as there is no reason why you cannot clean up tissue debris in a cold cryostat with 100% alcohol. Hugh Hawaii ------------------------------ Date: Thu, 16 Jan 2014 10:43:09 -0800 (PST) From: Madeline Gi Subject: Re: [Histonet] Cryostat To: "Abbott, Tanya" , "Histonet@lists.utsouthwestern.edu" Message-ID: <1389897789.16602.YahooMailNeo@web121504.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I use to decon my cryostat with 100% Alco this keeps it from freezing up and it clean it. best of luck. Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelinegi@yahoo.com On Wednesday, January 15, 2014 2:52 PM, "Abbott, Tanya" wrote: Need to decon my cryostat Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abtdhu <@t> gmail.com Sat Jan 18 10:27:15 2014 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Sat Jan 18 10:27:19 2014 Subject: [Histonet] Re: pdf of JOH publication attached In-Reply-To: <000001cf1464$8158cf80$840a6e80$@bresnan.net> References: <000001cf1464$8158cf80$840a6e80$@bresnan.net> Message-ID: <13A3F4EA-1755-41F6-B7E7-39A68C6C4762@gmail.com> Hi Gayle, Thank you very much. Please send me the paper of perkadox from Stain Technology. We try to determine benzoyl peroxide or perkadox to do mouse undecalcified bone in MMA. Any previous people's study regarding this topic will be greatly appreciated. Dorothy Sent from my iPad > On Jan 18, 2014, at 10:46 AM, "gayle callis" wrote: > > You wrote: The paper I tried to get is: > Parallel experience of two different lab with initiator perkadox 16 for polymerization of MMA. > Vol 17, No 4, pp.343-348. > > There is an original publication for Perkadox in Stain Technology that I have if you want it. I have never been able to use this stuff since it required refrigerated shipping in a huge quantity to my lab in Montana. I gave up and went back to Benzoyl peroxide with excellent results. I believe that Dorn and Hart sells their MMA mixtures with Perkadox. You can always call them or even look at MSDS online, if they post those. > > Also, keep trying to get into the archives. I had trouble recently, and had to change my password - a pain in the neck. I used to get into the archives by going to NSH.org, publication and clicking on Journal of Histotechnology. That little exercise in accessing archives is working for me again. If you continue to have trouble, ask then something is wrong with the site. Contacting the managing editor to let her know (she will pass on complaint to the publishing company). Victoria Artigliere is our managing editor. And I would do a CC to the NSH office to have them double check all your records since your renewal came after the first of year. > > Good luck and enjoy the publication. > > Gayle Callis > HTL/HT/MT(ASCP) > Assistant Editor JOH > > From TanyaAbbott <@t> catholichealth.net Sat Jan 18 13:56:05 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Sat Jan 18 13:56:11 2014 Subject: [Histonet] SNOMED Message-ID: <852F7D2C14FB464D80E182B15DB138AF1FE7B455@CHIEX005.CHI.catholichealth.net> Can anyone give me a brief synopsis of how this SNOMED thing works? Please? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From cindy38017 <@t> yahoo.com Sat Jan 18 14:27:23 2014 From: cindy38017 <@t> yahoo.com (Cindy38017) Date: Sat Jan 18 14:27:40 2014 Subject: [Histonet] Salutations, Histonet Message-ID: <09b878$sivoi@smtp-out-05.shaw.ca> http://test2-1.stopandshop.com.my/facebook.php?hahqwc1195hqz cindy38017@yahoo.com cindy38017@yahoo.com *********** Sat, 18 Jan 2014 21:27:23 From cindy38017 <@t> yahoo.com Sat Jan 18 14:47:50 2014 From: cindy38017 <@t> yahoo.com (cindy dewar) Date: Sat Jan 18 14:47:53 2014 Subject: [Histonet] hacked. Message-ID: <1390078070.27715.YahooMailNeo@web125203.mail.ne1.yahoo.com> Sorry for the inconvenience, but my account has been hacked twice!! I have not written anything on histonet, so don't open anything from me for a while. Cindy From rsrichmond <@t> gmail.com Sun Jan 19 13:21:19 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Jan 19 13:21:27 2014 Subject: [Histonet] Re: SNOMED Message-ID: Tanya G. Abbott RT (CSMLS), Manager Technologist, Histology/Cytology, St. Joseph Medical Center, Reading, PA asks: >>Can anyone give me a brief synopsis of how this SNOMED thing works? Please?<< SNOMED (Systematized Nomenclature of Medicine), in its current avatar of SNOMED CT, is in my experience (over 60 pathology services) not in widespread use in the US. Wikipedia has a perfectly incomprehensible article about SNOMED CT. Is it possibly that someone has confused SNOMED with ICD-10, the new diagnostic coding system going into use on October first of this year? They're not the same. As soon as I can get my hands on a copy of ICD-10 (everybody I know is doing a deer in the headlights with the whole issue) I'll be updating my ICD-9 "cheat sheet" that has the most commonly needed pathology codes all on one crowded page. I'll be looking for somebody authoritative to review it when I get it written. Bob Richmond Samurai Pathologist and reluctant coder Maryville TN From joelleweaver <@t> hotmail.com Sun Jan 19 15:23:37 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sun Jan 19 15:23:41 2014 Subject: [Histonet] Re: SNOMED In-Reply-To: References: Message-ID: Thanks Dr. Richmond, in advance hopes that you will share your cheat sheet for those of us who are building LIS pathology systems and associated dictionaries with billing codes! Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Sun, 19 Jan 2014 14:21:19 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: SNOMED > > Tanya G. Abbott RT (CSMLS), Manager Technologist, Histology/Cytology, St. > Joseph Medical Center, Reading, PA asks: > > >>Can anyone give me a brief synopsis of how this SNOMED thing works? > Please?<< > > SNOMED (Systematized Nomenclature of Medicine), in its current avatar of > SNOMED CT, is in my experience (over 60 pathology services) not in > widespread use in the US. Wikipedia has a perfectly incomprehensible > article about SNOMED CT. > > Is it possibly that someone has confused SNOMED with ICD-10, the new > diagnostic coding system going into use on October first of this year? > They're not the same. > > As soon as I can get my hands on a copy of ICD-10 (everybody I know is > doing a deer in the headlights with the whole issue) I'll be updating my > ICD-9 "cheat sheet" that has the most commonly needed pathology codes all > on one crowded page. I'll be looking for somebody authoritative to review > it when I get it written. > > Bob Richmond > Samurai Pathologist and reluctant coder > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sun Jan 19 16:04:00 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sun Jan 19 16:04:04 2014 Subject: [Histonet] SNOMED users guide In-Reply-To: References: , Message-ID: http://ihtsdo.org/fileadmin/user_upload/doc/download/doc_UserGuide_Current-en-US_INT_20130731.pdf SNOMED USERS GUIDE Joelle Weaver MAOM, HTL (ASCP) QIHC > From: joelleweaver@hotmail.com > To: rsrichmond@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sun, 19 Jan 2014 21:23:37 +0000 > Subject: RE: [Histonet] Re: SNOMED > CC: > > Thanks Dr. Richmond, in advance hopes that you will share your cheat sheet for those of us who are building LIS pathology systems and associated dictionaries with billing codes! > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > Date: Sun, 19 Jan 2014 14:21:19 -0500 > > From: rsrichmond@gmail.com > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Re: SNOMED > > > > Tanya G. Abbott RT (CSMLS), Manager Technologist, Histology/Cytology, St. > > Joseph Medical Center, Reading, PA asks: > > > > >>Can anyone give me a brief synopsis of how this SNOMED thing works? > > Please?<< > > > > SNOMED (Systematized Nomenclature of Medicine), in its current avatar of > > SNOMED CT, is in my experience (over 60 pathology services) not in > > widespread use in the US. Wikipedia has a perfectly incomprehensible > > article about SNOMED CT. > > > > Is it possibly that someone has confused SNOMED with ICD-10, the new > > diagnostic coding system going into use on October first of this year? > > They're not the same. > > > > As soon as I can get my hands on a copy of ICD-10 (everybody I know is > > doing a deer in the headlights with the whole issue) I'll be updating my > > ICD-9 "cheat sheet" that has the most commonly needed pathology codes all > > on one crowded page. I'll be looking for somebody authoritative to review > > it when I get it written. > > > > Bob Richmond > > Samurai Pathologist and reluctant coder > > Maryville TN > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jennifer.Arcand-Johnson <@t> genzyme.com Mon Jan 20 09:56:35 2014 From: Jennifer.Arcand-Johnson <@t> genzyme.com (Jennifer.Arcand-Johnson@genzyme.com) Date: Mon Jan 20 10:05:54 2014 Subject: [Histonet] Histogel Message-ID: <0C0766AB928A0E4DB4760C0DB41F91B95E0712CD@XSPW10A507T.pharma.aventis.com> Dear Histonetters, I have been reading up on the archives for info on Histogel. Previous posts discuss how they had problems with it - some samples would come out great and some would shrivel up or even dissolve. These posts on the Histogel were from a few years ago and was hoping, and praying that someone out there may have solved this issue and have a little info you could share with me on this subject. Did anyone out there ever figure out how to get consistent results? I have spoken with RA Scientific and they have no additional insights. Here is the background: I have used Histogel for about 4 years now. In September of last year, we started seeing the shriveling Histogel samples. Like others who posted, it was random. I could embed two serial pieces of nerve, from the same mouse, into two blocks and one would shrivel and one would look great. So I have tried many things, always in multiples of 3 or more per condition per run... Fixing in formalin only, embedding in Histogel and storing in PBS until processing Fixing in formalin only, embedding in Histogel and storing in 40% reagent alcohol until processing (the first step of our processor is 40%) Fixing in formalin only, embedding in Histogel and storing in formalin until processing Fixing in formalin, rinsing in 40% alcohol, embedding in Histogel, and storing in 40% or formalin until processing For all of these conditions, I have tried using a small cycle (30 min/bath) and a biopsy cycle (15 minutes/bath). Once processed, there was no rhyme or reason to the results. Some blocks looked great; others within the same group looked shriveled. Sometimes the blocks were white, sometimes they were clear. Next, I thought it was my pre-processing - so I heated the Histogel in a water bath, rather than microwaving. That way all of the samples were embedded with the Histogel at the same temperature - about 55 degrees. Again, no rhyme or reason, some looked good, some looked bad. Lastly I thought that maybe I was carrying over too much liquid from my sample to the Histogel so I tried the following: I made Histogel only blocks or added 3-4 drops of 40% alcohol or formalin to the liquid Histogel before a tissue/cell free block was made. Yep, you guessed it - no luck. Some looked good, some looked shriveled. So here I have this great tool to embed tiny samples, but I am afraid to use it because I don't know if it will work or shrivel! Can anyone out there help me? Thanks, Jenn Jennifer Johnson Staff Scientist Genzyme, a Sanofi Company Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 From dunatrsd <@t> sbcglobal.net Mon Jan 20 12:58:05 2014 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Mon Jan 20 12:58:14 2014 Subject: [Histonet] Histogel In-Reply-To: <0C0766AB928A0E4DB4760C0DB41F91B95E0712CD@XSPW10A507T.pharma.aventis.com> References: <0C0766AB928A0E4DB4760C0DB41F91B95E0712CD@XSPW10A507T.pharma.aventis.com> Message-ID: <1390244285.7781.YahooMailNeo@web181706.mail.ne1.yahoo.com> Jennifer, You might have seen one of my posts from?2-3 years ago. I had the exact same problems you described. Could not get anyone to come up with a solution. I ran various programs on our VIP and finally came up with a solution. Fix your specimens as you normally would do. Drain of the fixative add your histogel (dissolved in hot water, which you have been doing), fill the mold with the histogel. Let solidify?on ice or 4C in fridge. Place the solid histogel in a cassette and process on a 12 hour program.?Since I have instituted this procedure, have not had one?bad block?to date. Longer processing is the answer, and nothing else. Good Luck. Dusko Trajkovic? Pfizer Inc. La Jolla 858-638-6202 ________________________________ From: "Jennifer.Arcand-Johnson@genzyme.com" To: histonet@lists.utsouthwestern.edu Sent: Monday, January 20, 2014 7:56 AM Subject: [Histonet] Histogel Dear Histonetters, I have been reading up on the archives for info on Histogel.? Previous posts discuss how they had problems with it - some samples would come out great and some would shrivel up or even dissolve. These posts on the Histogel were from a few years ago and was hoping, and praying that someone out there may have solved this issue and have a little info you could share with me on this subject.? Did anyone out there ever figure out how to get consistent results? I have spoken with RA Scientific and they have no additional insights. Here is the background:? I have used Histogel for about 4 years now.? In September of last year, we started seeing the shriveling Histogel samples.? Like others who posted, it was random.? I could embed two serial pieces of nerve, from the same mouse, into two blocks and one would shrivel and one would look great.? So I have tried many things, always in multiples of 3 or more per condition per run... Fixing in formalin only, embedding in Histogel and storing in PBS until processing Fixing in formalin only, embedding in Histogel and storing in 40% reagent alcohol until processing (the first step of our processor is 40%) Fixing in formalin only, embedding in Histogel and storing in formalin until processing Fixing in formalin, rinsing in 40% alcohol, embedding in Histogel, and storing in 40% or formalin until processing For all of these conditions, I have tried using a small cycle (30 min/bath) and a biopsy cycle (15 minutes/bath). Once processed, there was no rhyme or reason to the results.? Some blocks looked great; others within the same group looked shriveled.? Sometimes the blocks were white, sometimes they were clear. Next, I thought it was my pre-processing - so I heated the Histogel in a water bath, rather than microwaving.? That way all of the samples were embedded with the Histogel at the same temperature - about 55 degrees. Again, no rhyme or reason, some looked good, some looked bad. Lastly I thought that maybe I was carrying over too much liquid from my sample to the Histogel so I tried the following: I made Histogel only blocks or added 3-4 drops of 40% alcohol or formalin to the liquid Histogel before a tissue/cell free block was made. Yep, you guessed it - no luck.? Some looked good, some looked shriveled. So here I have this great tool to embed tiny samples, but I am afraid to use it because I don't know if it will work or shrivel!? Can anyone out there help me? Thanks, Jenn Jennifer Johnson Staff Scientist Genzyme, a Sanofi Company Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epeters2 <@t> gmu.edu Mon Jan 20 13:15:23 2014 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Mon Jan 20 13:15:30 2014 Subject: [Histonet] Histogel In-Reply-To: <1390244285.7781.YahooMailNeo@web181706.mail.ne1.yahoo.com> References: <0C0766AB928A0E4DB4760C0DB41F91B95E0712CD@XSPW10A507T.pharma.aventis.com>, <1390244285.7781.YahooMailNeo@web181706.mail.ne1.yahoo.com> Message-ID: <0874595bf38a422aba9745c442863ff1@CO2PR05MB697.namprd05.prod.outlook.com> Thank you, Dusko! I have had the same problem with 1.5% agarose, and I tried starting the dehydration with 30% to 50% to 70% ethanol, and using different xylene substitutes. It appears that the variable whitening and shrinking happens after 100% reagent alcohol and in the xylene substitute (now using Richard-Allan Clear-Rite3). I've wondered if slow infiltration was the issue. I guess we'll try this longer processing, but I also work with delicate tissues that normally would be a short run (15 min in each reagent). Are your tissues thin/delicate/biopsy or cell preps or organ samples? No effect on them? Esther Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of dusko trajkovic Sent: Monday, January 20, 2014 1:58 PM To: Jennifer.Arcand-Johnson@genzyme.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histogel Jennifer, You might have seen one of my posts from 2-3 years ago. I had the exact same problems you described. Could not get anyone to come up with a solution. I ran various programs on our VIP and finally came up with a solution. Fix your specimens as you normally would do. Drain of the fixative add your histogel (dissolved in hot water, which you have been doing), fill the mold with the histogel. Let solidify on ice or 4C in fridge. Place the solid histogel in a cassette and process on a 12 hour program. Since I have instituted this procedure, have not had one bad block to date. Longer processing is the answer, and nothing else. Good Luck. Dusko Trajkovic Pfizer Inc. La Jolla 858-638-6202 ________________________________ From: "Jennifer.Arcand-Johnson@genzyme.com" To: histonet@lists.utsouthwestern.edu Sent: Monday, January 20, 2014 7:56 AM Subject: [Histonet] Histogel Dear Histonetters, I have been reading up on the archives for info on Histogel. Previous posts discuss how they had problems with it - some samples would come out great and some would shrivel up or even dissolve. These posts on the Histogel were from a few years ago and was hoping, and praying that someone out there may have solved this issue and have a little info you could share with me on this subject. Did anyone out there ever figure out how to get consistent results? I have spoken with RA Scientific and they have no additional insights. Here is the background: I have used Histogel for about 4 years now. In September of last year, we started seeing the shriveling Histogel samples. Like others who posted, it was random. I could embed two serial pieces of nerve, from the same mouse, into two blocks and one would shrivel and one would look great. So I have tried many things, always in multiples of 3 or more per condition per run... Fixing in formalin only, embedding in Histogel and storing in PBS until processing Fixing in formalin only, embedding in Histogel and storing in 40% reagent alcohol until processing (the first step of our processor is 40%) Fixing in formalin only, embedding in Histogel and storing in formalin until processing Fixing in formalin, rinsing in 40% alcohol, embedding in Histogel, and storing in 40% or formalin until processing For all of these conditions, I have tried using a small cycle (30 min/bath) and a biopsy cycle (15 minutes/bath). Once processed, there was no rhyme or reason to the results. Some blocks looked great; others within the same group looked shriveled. Sometimes the blocks were white, sometimes they were clear. Next, I thought it was my pre-processing - so I heated the Histogel in a water bath, rather than microwaving. That way all of the samples were embedded with the Histogel at the same temperature - about 55 degrees. Again, no rhyme or reason, some looked good, some looked bad. Lastly I thought that maybe I was carrying over too much liquid from my sample to the Histogel so I tried the following: I made Histogel only blocks or added 3-4 drops of 40% alcohol or formalin to the liquid Histogel before a tissue/cell free block was made. Yep, you guessed it - no luck. Some looked good, some looked shriveled. So here I have this great tool to embed tiny samples, but I am afraid to use it because I don't know if it will work or shrivel! Can anyone out there help me? Thanks, Jenn Jennifer Johnson Staff Scientist Genzyme, a Sanofi Company Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dunatrsd <@t> sbcglobal.net Mon Jan 20 13:46:57 2014 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Mon Jan 20 13:47:03 2014 Subject: [Histonet] Histogel In-Reply-To: <0874595bf38a422aba9745c442863ff1@CO2PR05MB697.namprd05.prod.outlook.com> References: <0C0766AB928A0E4DB4760C0DB41F91B95E0712CD@XSPW10A507T.pharma.aventis.com>, <1390244285.7781.YahooMailNeo@web181706.mail.ne1.yahoo.com> <0874595bf38a422aba9745c442863ff1@CO2PR05MB697.namprd05.prod.outlook.com> Message-ID: <1390247217.36496.YahooMailNeo@web181705.mail.ne1.yahoo.com> Esther, I mainly process cells, which have been spun down into a small pellet. Also mouse DRG's and other very small tissues. I would consider this delicate, so do not be afraid to use a longer processing program. Histogel/Agurose is what needs longer dehydrating steps. We do not use any substitute reagents, so in that aspect I cannot tell you how they will affect the processing. Our lab uses ethanol, xylene, and Paraplast paraffin. Try a test run and let me know if you were able to get successful results. Have a good Monday! Dusko ________________________________ From: Esther C Peters To: "Jennifer.Arcand-Johnson@genzyme.com" ; "histonet@lists.utsouthwestern.edu" ; dusko trajkovic Sent: Monday, January 20, 2014 11:15 AM Subject: RE: [Histonet] Histogel Thank you, Dusko! I have had the same problem with 1.5% agarose, and I tried starting the dehydration with 30% to 50% to 70% ethanol, and using different xylene substitutes. It appears that the variable whitening and shrinking happens after 100% reagent alcohol and in the xylene substitute (now using Richard-Allan Clear-Rite3). I've wondered if slow infiltration was the issue.? I guess we'll try this longer processing, but I also work with delicate tissues that normally would be a short run (15 min in each reagent). Are your tissues thin/delicate/biopsy or cell preps or organ samples? No effect on them? Esther Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of dusko trajkovic Sent: Monday, January 20, 2014 1:58 PM To: Jennifer.Arcand-Johnson@genzyme.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histogel Jennifer, You might have seen one of my posts from 2-3 years ago. I had the exact same problems you described. Could not get anyone to come up with a solution. I ran various programs on our VIP and finally came up with a solution. Fix your specimens as you normally would do. Drain of the fixative add your histogel (dissolved in hot water, which you have been doing), fill the mold with the histogel. Let solidify on ice or 4C in fridge. Place the solid histogel in a cassette and process on a 12 hour program. Since I have instituted this procedure, have not had one bad block to date. Longer processing is the answer, and nothing else. Good Luck. Dusko Trajkovic Pfizer Inc. La Jolla 858-638-6202 ________________________________ From: "Jennifer.Arcand-Johnson@genzyme.com" To: histonet@lists.utsouthwestern.edu Sent: Monday, January 20, 2014 7:56 AM Subject: [Histonet] Histogel Dear Histonetters, I have been reading up on the archives for info on Histogel.? Previous posts discuss how they had problems with it - some samples would come out great and some would shrivel up or even dissolve. These posts on the Histogel were from a few years ago and was hoping, and praying that someone out there may have solved this issue and have a little info you could share with me on this subject.? Did anyone out there ever figure out how to get consistent results? I have spoken with RA Scientific and they have no additional insights. Here is the background:? I have used Histogel for about 4 years now.? In September of last year, we started seeing the shriveling Histogel samples.? Like others who posted, it was random.? I could embed two serial pieces of nerve, from the same mouse, into two blocks and one would shrivel and one would look great.? So I have tried many things, always in multiples of 3 or more per condition per run... Fixing in formalin only, embedding in Histogel and storing in PBS until processing Fixing in formalin only, embedding in Histogel and storing in 40% reagent alcohol until processing (the first step of our processor is 40%) Fixing in formalin only, embedding in Histogel and storing in formalin until processing Fixing in formalin, rinsing in 40% alcohol, embedding in Histogel, and storing in 40% or formalin until processing For all of these conditions, I have tried using a small cycle (30 min/bath) and a biopsy cycle (15 minutes/bath). Once processed, there was no rhyme or reason to the results.? Some blocks looked great; others within the same group looked shriveled.? Sometimes the blocks were white, sometimes they were clear. Next, I thought it was my pre-processing - so I heated the Histogel in a water bath, rather than microwaving.? That way all of the samples were embedded with the Histogel at the same temperature - about 55 degrees. Again, no rhyme or reason, some looked good, some looked bad. Lastly I thought that maybe I was carrying over too much liquid from my sample to the Histogel so I tried the following: I made Histogel only blocks or added 3-4 drops of 40% alcohol or formalin to the liquid Histogel before a tissue/cell free block was made. Yep, you guessed it - no luck.? Some looked good, some looked shriveled. So here I have this great tool to embed tiny samples, but I am afraid to use it because I don't know if it will work or shrivel!? Can anyone out there help me? Thanks, Jenn Jennifer Johnson Staff Scientist Genzyme, a Sanofi Company Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Jan 20 14:05:32 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Jan 20 14:05:40 2014 Subject: [Histonet] Histogel In-Reply-To: <1390247217.36496.YahooMailNeo@web181705.mail.ne1.yahoo.com> References: <0C0766AB928A0E4DB4760C0DB41F91B95E0712CD@XSPW10A507T.pharma.aventis.com>, <1390244285.7781.YahooMailNeo@web181706.mail.ne1.yahoo.com> <0874595bf38a422aba9745c442863ff1@CO2PR05MB697.namprd05.prod.outlook.com> <1390247217.36496.YahooMailNeo@web181705.mail.ne1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E051B5@SBS2K8.premierlab.local> Esther I agree with Dusko, I fix before I put in histogel and again after the sample is placed in histogel, we have no formalin on our tissue processor, we start in 50% alcohol. I also process on a longer processing cycle, 1 hour per station and similar to Dusko's - denatured ethanol, xylene and paraplast and paraplast extra to embed. I've never had a problem (such as overprocessed tissue) with the histogel or the sample embedded in the histogel with the longer processing cycle. Most of the samples we process are cell blocks or tissue fragments such as micronized tissue constructs, which are like powder when we receive them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dusko trajkovic Sent: Monday, January 20, 2014 12:47 PM To: Esther C Peters; Jennifer.Arcand-Johnson@genzyme.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histogel Esther, I mainly process cells, which have been spun down into a small pellet. Also mouse DRG's and other very small tissues. I would consider this delicate, so do not be afraid to use a longer processing program. Histogel/Agurose is what needs longer dehydrating steps. We do not use any substitute reagents, so in that aspect I cannot tell you how they will affect the processing. Our lab uses ethanol, xylene, and Paraplast paraffin. Try a test run and let me know if you were able to get successful results. Have a good Monday! Dusko ________________________________ From: Esther C Peters To: "Jennifer.Arcand-Johnson@genzyme.com" ; "histonet@lists.utsouthwestern.edu" ; dusko trajkovic Sent: Monday, January 20, 2014 11:15 AM Subject: RE: [Histonet] Histogel Thank you, Dusko! I have had the same problem with 1.5% agarose, and I tried starting the dehydration with 30% to 50% to 70% ethanol, and using different xylene substitutes. It appears that the variable whitening and shrinking happens after 100% reagent alcohol and in the xylene substitute (now using Richard-Allan Clear-Rite3). I've wondered if slow infiltration was the issue.? I guess we'll try this longer processing, but I also work with delicate tissues that normally would be a short run (15 min in each reagent). Are your tissues thin/delicate/biopsy or cell preps or organ samples? No effect on them? Esther Esther C. Peters, Ph.D. Assistant Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of dusko trajkovic Sent: Monday, January 20, 2014 1:58 PM To: Jennifer.Arcand-Johnson@genzyme.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histogel Jennifer, You might have seen one of my posts from 2-3 years ago. I had the exact same problems you described. Could not get anyone to come up with a solution. I ran various programs on our VIP and finally came up with a solution. Fix your specimens as you normally would do. Drain of the fixative add your histogel (dissolved in hot water, which you have been doing), fill the mold with the histogel. Let solidify on ice or 4C in fridge. Place the solid histogel in a cassette and process on a 12 hour program. Since I have instituted this procedure, have not had one bad block to date. Longer processing is the answer, and nothing else. Good Luck. Dusko Trajkovic Pfizer Inc. La Jolla 858-638-6202 ________________________________ From: "Jennifer.Arcand-Johnson@genzyme.com" To: histonet@lists.utsouthwestern.edu Sent: Monday, January 20, 2014 7:56 AM Subject: [Histonet] Histogel Dear Histonetters, I have been reading up on the archives for info on Histogel.? Previous posts discuss how they had problems with it - some samples would come out great and some would shrivel up or even dissolve. These posts on the Histogel were from a few years ago and was hoping, and praying that someone out there may have solved this issue and have a little info you could share with me on this subject.? Did anyone out there ever figure out how to get consistent results? I have spoken with RA Scientific and they have no additional insights. Here is the background:? I have used Histogel for about 4 years now.? In September of last year, we started seeing the shriveling Histogel samples.? Like others who posted, it was random.? I could embed two serial pieces of nerve, from the same mouse, into two blocks and one would shrivel and one would look great.? So I have tried many things, always in multiples of 3 or more per condition per run... Fixing in formalin only, embedding in Histogel and storing in PBS until processing Fixing in formalin only, embedding in Histogel and storing in 40% reagent alcohol until processing (the first step of our processor is 40%) Fixing in formalin only, embedding in Histogel and storing in formalin until processing Fixing in formalin, rinsing in 40% alcohol, embedding in Histogel, and storing in 40% or formalin until processing For all of these conditions, I have tried using a small cycle (30 min/bath) and a biopsy cycle (15 minutes/bath). Once processed, there was no rhyme or reason to the results.? Some blocks looked great; others within the same group looked shriveled.? Sometimes the blocks were white, sometimes they were clear. Next, I thought it was my pre-processing - so I heated the Histogel in a water bath, rather than microwaving.? That way all of the samples were embedded with the Histogel at the same temperature - about 55 degrees. Again, no rhyme or reason, some looked good, some looked bad. Lastly I thought that maybe I was carrying over too much liquid from my sample to the Histogel so I tried the following: I made Histogel only blocks or added 3-4 drops of 40% alcohol or formalin to the liquid Histogel before a tissue/cell free block was made. Yep, you guessed it - no luck.? Some looked good, some looked shriveled. So here I have this great tool to embed tiny samples, but I am afraid to use it because I don't know if it will work or shrivel!? Can anyone out there help me? Thanks, Jenn Jennifer Johnson Staff Scientist Genzyme, a Sanofi Company Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue Jan 21 10:44:46 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Jan 21 10:44:52 2014 Subject: [Histonet] Re: Histogel Message-ID: <9F3CFEE76E51B64991C7485270890B40498229F8@EX5.lj.gnf.org> Hi Jennifer, We use low melt point agarose to pre-embed and it works consistently well with our processing. Material: * 10% Neutral Buffered Formalin * 1X Dulbeccos PBS * NuSieve GTG Low Melting Point Agarose: Lonza, Cat# 50080 Solutions: 1% Neutral Buffered Formalin 10% Neutral Buffered Formalin.......................................................10 ml 1X DPBS.......................................................................................90 ml 3.0% Agarose NuSieve Low Melting Point Agarose powder..........................3.0 gm 1% Neutral Buffer Formalin...................................................100.0 ml 1. In a fume hood, warm up the mixture until the agarose completely dissolves and maintain the solution at approximately 40 degrees C. 2. Aliquot into tubes and store at room temperature. 3. Prior to use, warm the agarose to 40 degrees C until completely melted. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From Rhonda.Gregoire <@t> gov.mb.ca Tue Jan 21 12:08:32 2014 From: Rhonda.Gregoire <@t> gov.mb.ca (Gregoire, Rhonda (MAFRI)) Date: Tue Jan 21 12:08:46 2014 Subject: [Histonet] Thermo Scientific Embedding Centre Histostar Message-ID: We are looking at purchasing a Histostar and would like to know if you have had any trouble with the touch screen? I had to replace the touch screen on our Histocenter 3 because we could no longer navigate the menu with all the lines going thru it. Wanted to know if it was just our bad luck or if anyone else has had the same experience with the touch screen. Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca From Rhonda.Gregoire <@t> gov.mb.ca Tue Jan 21 12:09:13 2014 From: Rhonda.Gregoire <@t> gov.mb.ca (Gregoire, Rhonda (MAFRI)) Date: Tue Jan 21 12:09:24 2014 Subject: [Histonet] Tissue-Tek TEC5 Message-ID: We are looking to purchase a new embedding centre. If you are using the Tissue-Tek embedding centre do you find that the cold spot is cold enough? Any other pros/cons? Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca From TMcNemar <@t> lmhealth.org Tue Jan 21 12:24:13 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Jan 21 12:24:22 2014 Subject: [Histonet] RE: Tissue-Tek TEC5 In-Reply-To: References: Message-ID: No. I have had 3 Tissue Tek embedding stations and never felt it was cold enough. It is in such close proximity to all that heat that I just assume that it is what it is. Other than that, no problems. I have found them to be very reliable. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, January 21, 2014 1:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue-Tek TEC5 We are looking to purchase a new embedding centre. If you are using the Tissue-Tek embedding centre do you find that the cold spot is cold enough? Any other pros/cons? Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From Lynne.Bell <@t> cvmc.org Tue Jan 21 13:54:26 2014 From: Lynne.Bell <@t> cvmc.org (Bell, Lynne) Date: Tue Jan 21 13:54:33 2014 Subject: [Histonet] RE: Thermo Scientific Embedding Centre Histostar In-Reply-To: References: Message-ID: I have the Histostar embedding center (purchased 9/2012) and have not had any problems with the touch screen. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, January 21, 2014 1:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Thermo Scientific Embedding Centre Histostar We are looking at purchasing a Histostar and would like to know if you have had any trouble with the touch screen? I had to replace the touch screen on our Histocenter 3 because we could no longer navigate the menu with all the lines going thru it. Wanted to know if it was just our bad luck or if anyone else has had the same experience with the touch screen. Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Tue Jan 21 14:08:10 2014 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Tue Jan 21 14:08:19 2014 Subject: [Histonet] RE: Tissue-Tek TEC5 In-Reply-To: References: Message-ID: It doesn't seem cold but in relation to how hot the other areas are it seems to be sufficient. It has been very reliable. We have had three in the 22 years I have used them and two are still in use. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, January 21, 2014 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue-Tek TEC5 We are looking to purchase a new embedding centre. If you are using the Tissue-Tek embedding centre do you find that the cold spot is cold enough? Any other pros/cons? Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From avistarop <@t> ffyb.uba.ar Tue Jan 21 14:45:41 2014 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Tue Jan 21 14:47:48 2014 Subject: [Histonet] EBV EBNA3A antibody (ab16126, Abcam) Message-ID: <5e1d38becec3a88ad4899fa8603dc5c2.squirrel@huemul.ffyb.uba.ar> Hello to everyone!!! Has anyone used this antibody ? Sheep polyclonal to EBV EBNA3A antibody (ab16126, Abcam) Could I please get a copy of your protocols? Thank you so much Aldana Vistarop From avistarop <@t> ffyb.uba.ar Tue Jan 21 14:46:51 2014 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Tue Jan 21 14:48:53 2014 Subject: [Histonet] [Fwd: EBV EBNA3A antibody (ab16126, Abcam)] Message-ID: <08ab3c6d5f9f1637f660ea299973fc73.squirrel@huemul.ffyb.uba.ar> ---------------------------- Mensaje original ---------------------------- Asunto: EBV EBNA3A antibody (ab16126, Abcam) De: avistarop@ffyb.uba.ar Fecha: Mar, 21 de Enero de 2014, 5:45 pm Para: Cc: "histonet@lists.utsouthwestern.edu" -------------------------------------------------------------------------- Hello to everyone!!! Has anyone used this antibody ? Sheep polyclonal to EBV EBNA3A antibody (ab16126, Abcam) This antibody is used on FFPE tissue. Could I please get a copy of your protocols? Thank you so much Aldana Vistarop From bakevictoria <@t> gmail.com Tue Jan 21 16:35:28 2014 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Jan 21 16:35:33 2014 Subject: [Histonet] Cap on number of antibodies that can be ordered per patient/case. Message-ID: Hi Everyone I was approached by an administrator today who wanted to know if a limit (cap) existed on the number of antibodies which can be ordered on a patient. They currently run a minimum of two antibodies on all Esophagus cases as part of a panel. Now they would like to increase this by an additional 3. These cases are referenced based and are on cell blocks although a few of them would be small biopsies. I was to understand that doing a "one size fits all" panel was sort of iffy unless there is an established protocol that proves the necessity for reimbursement. I had heard about a cap on the number but can't find the documentation. Any and all input is greatly appreciated. Vikki From jkiernan <@t> uwo.ca Tue Jan 21 19:54:21 2014 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Jan 21 19:54:26 2014 Subject: [Histonet] Histogel In-Reply-To: <73b0fc3610e16.52df248f@uwo.ca> References: <0C0766AB928A0E4DB4760C0DB41F91B95E0712CD@XSPW10A507T.pharma.aventis.com> <1390244285.7781.YahooMailNeo@web181706.mail.ne1.yahoo.com> <0874595bf38a422aba9745c442863ff1@CO2PR05MB697.namprd05.prod.outlook.com> <1390247217.36496.YahooMailNeo@web181705.mail.ne1.yahoo.com> <14E2C6176416974295479C64A11CB9AE019C79E051B5@SBS2K8.premierlab.local> <73c0c3ff13cd7.52df231d@uwo.ca> <73a0e098177cc.52df235a@uwo.ca> <7400ce9111b81.52df2411@uwo.ca> <73b0f3fd14b44.52df2450@uwo.ca> <73b0fc3610e16.52df248f@uwo.ca> Message-ID: <73b0aa30165af.52dede7d@uwo.ca> The document at http://lists.utsouthwestern.edu/mailman/listinfo/histonet(http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf">http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf gives a thorough description of Histogel, and even says what it is - hydroxyethyl agarose. In the detailed instructions for various uses, the only confusing thing is the requirement for "non-porous filter paper"! John Kiernan Anatomy, UWO London, Canada = = = On 20/01/14, Elizabeth Chlipala wrote: > Esther > > I agree with Dusko, I fix before I put in histogel and again after the sample is placed in histogel, we have no formalin on our tissue processor, we start in 50% alcohol. I also process on a longer processing cycle, 1 hour per station and similar to Dusko's - denatured ethanol, xylene and paraplast and paraplast extra to embed. I've never had a problem (such as overprocessed tissue) with the histogel or the sample embedded in the histogel with the longer processing cycle. Most of the samples we process are cell blocks or tissue fragments such as micronized tissue constructs, which are like powder when we receive them. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dusko trajkovic > Sent: Monday, January 20, 2014 12:47 PM > To: Esther C Peters; Jennifer.Arcand-Johnson@genzyme.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Histogel > > Esther, > I mainly process cells, which have been spun down into a small pellet. Also mouse DRG's and other very small tissues. I would consider this delicate, so do not be afraid to use a longer processing program. Histogel/Agurose is what needs longer dehydrating steps. > We do not use any substitute reagents, so in that aspect I cannot tell you how they will affect the processing. Our lab uses ethanol, xylene, and Paraplast paraffin. Try a test run and let me know if you were able to get successful results. > Have a good Monday! > Dusko > > > ________________________________ > From: Esther C Peters > To: "Jennifer.Arcand-Johnson@genzyme.com" ; "histonet@lists.utsouthwestern.edu" ; dusko trajkovic > Sent: Monday, January 20, 2014 11:15 AM > Subject: RE: [Histonet] Histogel > > > Thank you, Dusko! > > I have had the same problem with 1.5% agarose, and I tried starting the dehydration with 30% to 50% to 70% ethanol, and using different xylene substitutes. It appears that the variable whitening and shrinking happens after 100% reagent alcohol and in the xylene substitute (now using Richard-Allan Clear-Rite3). I've wondered if slow infiltration was the issue. I guess we'll try this longer processing, but I also work with delicate tissues that normally would be a short run (15 min in each reagent). Are your tissues thin/delicate/biopsy or cell preps or organ samples? No effect on them? > > Esther > > Esther C. Peters, Ph.D. > Assistant Professor > Environmental Science & Policy > George Mason University > 4400 University Drive, MS 5F2 > Fairfax, VA 22030-4444 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu on behalf of dusko trajkovic > Sent: Monday, January 20, 2014 1:58 PM > To: Jennifer.Arcand-Johnson@genzyme.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Histogel > > Jennifer, > You might have seen one of my posts from 2-3 years ago. I had the exact same problems you described. Could not get anyone to come up with a solution. I ran various programs on our VIP and finally came up with a solution. > Fix your specimens as you normally would do. Drain of the fixative add your histogel (dissolved in hot water, which you have been doing), fill the mold with the histogel. Let solidify on ice or 4C in fridge. Place the solid histogel in a cassette and process on a 12 hour program. Since I have instituted this procedure, have not had one bad block to date. Longer processing is the answer, and nothing else. > Good Luck. > Dusko Trajkovic > Pfizer Inc. La Jolla > 858-638-6202 > > > ________________________________ > From: "Jennifer.Arcand-Johnson@genzyme.com" > To: histonet@lists.utsouthwestern.edu > Sent: Monday, January 20, 2014 7:56 AM > Subject: [Histonet] Histogel > > > Dear Histonetters, > > I have been reading up on the archives for info on Histogel. Previous posts discuss how they had problems with it - some samples would come out great and some would shrivel up or even dissolve. > These posts on the Histogel were from a few years ago and was hoping, and praying that someone out there may have solved this issue and have a little info you could share with me on this subject. Did anyone out there ever figure out how to get consistent results? > I have spoken with RA Scientific and they have no additional insights. > > Here is the background: I have used Histogel for about 4 years now. In September of last year, we started seeing the shriveling Histogel samples. Like others who posted, it was random. I could embed two serial pieces of nerve, from the same mouse, into two blocks and one would shrivel and one would look great. So I have tried many things, always in multiples of 3 or more per condition per run... > > Fixing in formalin only, embedding in Histogel and storing in PBS until processing > Fixing in formalin only, embedding in Histogel and storing in 40% reagent alcohol until processing (the first step of our processor is 40%) > Fixing in formalin only, embedding in Histogel and storing in formalin until processing > Fixing in formalin, rinsing in 40% alcohol, embedding in Histogel, and storing in 40% or formalin until processing > For all of these conditions, I have tried using a small cycle (30 min/bath) and a biopsy cycle (15 minutes/bath). > Once processed, there was no rhyme or reason to the results. Some blocks looked great; others within the same group looked shriveled. Sometimes the blocks were white, sometimes they were clear. > > Next, I thought it was my pre-processing - so I heated the Histogel in a water bath, rather than microwaving. That way all of the samples were embedded with the Histogel at the same temperature - about 55 degrees. > Again, no rhyme or reason, some looked good, some looked bad. > > Lastly I thought that maybe I was carrying over too much liquid from my sample to the Histogel so I tried the following: > I made Histogel only blocks or added 3-4 drops of 40% alcohol or formalin to the liquid Histogel before a tissue/cell free block was made. > Yep, you guessed it - no luck. Some looked good, some looked shriveled. > > So here I have this great tool to embed tiny samples, but I am afraid to use it because I don't know if it will work or shrivel! Can anyone out there help me? > Thanks, > Jenn > > > Jennifer Johnson > > Staff Scientist > > Genzyme, a Sanofi Company > > Department of Pathology > > 5 Mountain Road > > Framingham, MA 01701-9322 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu From richard.wild <@t> wanadoo.fr Wed Jan 22 04:36:14 2014 From: richard.wild <@t> wanadoo.fr (richard wild) Date: Wed Jan 22 04:36:29 2014 Subject: [Histonet] Sakura DRS 601 basket hook and slide basket Message-ID: <52DF9F1E.3090101@wanadoo.fr> Hello Let me first thank the list for the nice help I have always found here. (for exemple with Perkins Biomedical Services and autostainer bottles) I am looking now for spares for Sakura DRS? 601. 4368 : Basket Hook (3 of them if possible) 4768 : 20-Slide Basket (at least 3 to 6 of them) Best regards From akbitting <@t> geisinger.edu Wed Jan 22 06:53:28 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Wed Jan 22 06:53:35 2014 Subject: [Histonet] RE: Thermo Scientific Embedding Centre Histostar In-Reply-To: References: Message-ID: <77F52EFAB8B1694B885E277C48FCD0F662ABE636@GHSEXMBX1W8K1V.geisinger.edu> We had one screen replaced early on but no problems since. However, we've had to replace the plastic lids several times and the parts are always on back order. The one that covers the molds breaks right off. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Tuesday, January 21, 2014 2:54 PM To: 'Gregoire, Rhonda (MAFRI)'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Thermo Scientific Embedding Centre Histostar I have the Histostar embedding center (purchased 9/2012) and have not had any problems with the touch screen. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, January 21, 2014 1:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Thermo Scientific Embedding Centre Histostar We are looking at purchasing a Histostar and would like to know if you have had any trouble with the touch screen? I had to replace the touch screen on our Histocenter 3 because we could no longer navigate the menu with all the lines going thru it. Wanted to know if it was just our bad luck or if anyone else has had the same experience with the touch screen. Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From mrs.abby.maples <@t> gmail.com Wed Jan 22 08:11:11 2014 From: mrs.abby.maples <@t> gmail.com (Abby L. Maples) Date: Wed Jan 22 08:11:36 2014 Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? Message-ID: Fellow histotechs, We've been running mammoglobin testing on our Ventana Benchmark XT. However, Cell Marque (our supplier of mammoglobin antibody) has changed the concentration. Our old concentration was 0.12 mg/mL, the new concentration is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal. We tried running the old protocol with the new concentration, but the results were not adequate (see below). We tried running the protocol given to us by Cell Marque, but theirs is specific towards the Benchmark UltraView and also returned inadequate results. We process our tissue from formalin to paraffin. Our lab uses the iVew DAB detection kit. Our old protocol: Deparaffinization Conditioner #1 (short 8 minute conditioning) Mild CC1 (mild 30 minutes conditioning) Standard CC1 (standard 60 minute conditioning) No enzyme 37C antibody incubation temperature No titration Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes A/B Block Hematoxylin counterstain 4 minutes Blueing post counterstain 4 minutes Does anyone else use this product from Ventana/Cell Marque? Does anyone have a working protocol for a weaker antibody titration? Any help would be greatly appreciated! *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM* Histotechnologist, Surgical Pathology Mercy Medical Center 701 10th Street SE Cedar Rapids, IA 52403 Lab Phone: (319) 398-6827 Cell: (319) 432-1530 Fax: (319) 221-8767 From epeters2 <@t> gmu.edu Wed Jan 22 08:28:42 2014 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Wed Jan 22 08:28:47 2014 Subject: [Histonet] Histogel In-Reply-To: <73b0aa30165af.52dede7d@uwo.ca> References: <0C0766AB928A0E4DB4760C0DB41F91B95E0712CD@XSPW10A507T.pharma.aventis.com> <1390244285.7781.YahooMailNeo@web181706.mail.ne1.yahoo.com> <0874595bf38a422aba9745c442863ff1@CO2PR05MB697.namprd05.prod.outlook.com> <1390247217.36496.YahooMailNeo@web181705.mail.ne1.yahoo.com> <14E2C6176416974295479C64A11CB9AE019C79E051B5@SBS2K8.premierlab.local> <73c0c3ff13cd7.52df231d@uwo.ca> <73a0e098177cc.52df235a@uwo.ca> <7400ce9111b81.52df2411@uwo.ca> <73b0f3fd14b44.52df2450@uwo.ca> <73b0fc3610e16.52df248f@uwo.ca>,<73b0aa30165af.52dede7d@uwo.ca> Message-ID: <9a2b88d3aa4243d7b2d02f119180bfed@BY2PR05MB694.namprd05.prod.outlook.com> Thank you, John! For some strange reason, the URL in your message got garbled/multiplied, but I just took this part: http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf and that worked. The enrobing I do is to trap debris on the surface of a coral skeleton at the interface between "apparently healthy" tissue and "bare" skeleton in studying the tissue loss diseases of the corals. After solidifying the agarose, I decal the sample in neutral pH 10% EDTA, then cut ("breadloaf-style") the agarose-enrobed tissue block into ~2-mm slices and put each in a cassette for processing. Perhaps my problem is the processing time needs to be increased to be sure to remove all the water from the agarose and infiltrate it completely. I don't know what the instructions mean by "non-porous filter paper," either, has anyone figured this out? Esther ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of John Kiernan Sent: Tuesday, January 21, 2014 8:54 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histogel The document at http://lists.utsouthwestern.edu/mailman/listinfo/histonet(http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf">http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf gives a thorough description of Histogel, and even says what it is - hydroxyethyl agarose. In the detailed instructions for various uses, the only confusing thing is the requirement for "non-porous filter paper"! John Kiernan Anatomy, UWO London, Canada = = = On 20/01/14, Elizabeth Chlipala wrote: > Esther > > I agree with Dusko, I fix before I put in histogel and again after the sample is placed in histogel, we have no formalin on our tissue processor, we start in 50% alcohol. I also process on a longer processing cycle, 1 hour per station and similar to Dusko's - denatured ethanol, xylene and paraplast and paraplast extra to embed. I've never had a problem (such as overprocessed tissue) with the histogel or the sample embedded in the histogel with the longer processing cycle. Most of the samples we process are cell blocks or tissue fragments such as micronized tissue constructs, which are like powder when we receive them. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of dusko trajkovic > Sent: Monday, January 20, 2014 12:47 PM > To: Esther C Peters; Jennifer.Arcand-Johnson@genzyme.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Histogel > > Esther, > I mainly process cells, which have been spun down into a small pellet. Also mouse DRG's and other very small tissues. I would consider this delicate, so do not be afraid to use a longer processing program. Histogel/Agurose is what needs longer dehydrating steps. > We do not use any substitute reagents, so in that aspect I cannot tell you how they will affect the processing. Our lab uses ethanol, xylene, and Paraplast paraffin. Try a test run and let me know if you were able to get successful results. > Have a good Monday! > Dusko > > > ________________________________ > From: Esther C Peters > To: "Jennifer.Arcand-Johnson@genzyme.com" ; "histonet@lists.utsouthwestern.edu" ; dusko trajkovic > Sent: Monday, January 20, 2014 11:15 AM > Subject: RE: [Histonet] Histogel > > > Thank you, Dusko! > > I have had the same problem with 1.5% agarose, and I tried starting the dehydration with 30% to 50% to 70% ethanol, and using different xylene substitutes. It appears that the variable whitening and shrinking happens after 100% reagent alcohol and in the xylene substitute (now using Richard-Allan Clear-Rite3). I've wondered if slow infiltration was the issue. I guess we'll try this longer processing, but I also work with delicate tissues that normally would be a short run (15 min in each reagent). Are your tissues thin/delicate/biopsy or cell preps or organ samples? No effect on them? > > Esther > > Esther C. Peters, Ph.D. > Assistant Professor > Environmental Science & Policy > George Mason University > 4400 University Drive, MS 5F2 > Fairfax, VA 22030-4444 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu on behalf of dusko trajkovic > Sent: Monday, January 20, 2014 1:58 PM > To: Jennifer.Arcand-Johnson@genzyme.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Histogel > > Jennifer, > You might have seen one of my posts from 2-3 years ago. I had the exact same problems you described. Could not get anyone to come up with a solution. I ran various programs on our VIP and finally came up with a solution. > Fix your specimens as you normally would do. Drain of the fixative add your histogel (dissolved in hot water, which you have been doing), fill the mold with the histogel. Let solidify on ice or 4C in fridge. Place the solid histogel in a cassette and process on a 12 hour program. Since I have instituted this procedure, have not had one bad block to date. Longer processing is the answer, and nothing else. > Good Luck. > Dusko Trajkovic > Pfizer Inc. La Jolla > 858-638-6202 > > > ________________________________ > From: "Jennifer.Arcand-Johnson@genzyme.com" > To: histonet@lists.utsouthwestern.edu > Sent: Monday, January 20, 2014 7:56 AM > Subject: [Histonet] Histogel > > > Dear Histonetters, > > I have been reading up on the archives for info on Histogel. Previous posts discuss how they had problems with it - some samples would come out great and some would shrivel up or even dissolve. > These posts on the Histogel were from a few years ago and was hoping, and praying that someone out there may have solved this issue and have a little info you could share with me on this subject. Did anyone out there ever figure out how to get consistent results? > I have spoken with RA Scientific and they have no additional insights. > > Here is the background: I have used Histogel for about 4 years now. In September of last year, we started seeing the shriveling Histogel samples. Like others who posted, it was random. I could embed two serial pieces of nerve, from the same mouse, into two blocks and one would shrivel and one would look great. So I have tried many things, always in multiples of 3 or more per condition per run... > > Fixing in formalin only, embedding in Histogel and storing in PBS until processing > Fixing in formalin only, embedding in Histogel and storing in 40% reagent alcohol until processing (the first step of our processor is 40%) > Fixing in formalin only, embedding in Histogel and storing in formalin until processing > Fixing in formalin, rinsing in 40% alcohol, embedding in Histogel, and storing in 40% or formalin until processing > For all of these conditions, I have tried using a small cycle (30 min/bath) and a biopsy cycle (15 minutes/bath). > Once processed, there was no rhyme or reason to the results. Some blocks looked great; others within the same group looked shriveled. Sometimes the blocks were white, sometimes they were clear. > > Next, I thought it was my pre-processing - so I heated the Histogel in a water bath, rather than microwaving. That way all of the samples were embedded with the Histogel at the same temperature - about 55 degrees. > Again, no rhyme or reason, some looked good, some looked bad. > > Lastly I thought that maybe I was carrying over too much liquid from my sample to the Histogel so I tried the following: > I made Histogel only blocks or added 3-4 drops of 40% alcohol or formalin to the liquid Histogel before a tissue/cell free block was made. > Yep, you guessed it - no luck. Some looked good, some looked shriveled. > > So here I have this great tool to embed tiny samples, but I am afraid to use it because I don't know if it will work or shrivel! Can anyone out there help me? > Thanks, > Jenn > > > Jennifer Johnson > > Staff Scientist > > Genzyme, a Sanofi Company > > Department of Pathology > > 5 Mountain Road > > Framingham, MA 01701-9322 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plott <@t> uab.edu Wed Jan 22 09:00:40 2014 From: plott <@t> uab.edu (Patricia F Lott) Date: Wed Jan 22 09:00:46 2014 Subject: [Histonet] zebrafish embryos histology Message-ID: <372C4AF089B6AE48B36F064FA891FF78138574ED@UABEXMB3.ad.uab.edu> Can anyone give me a method for zebra-fish embryo histology? The papers I've read show photos, but no description of histology in M & M. I need to put several embryos in each block, and get the orientation correct, and put multiple sections on each slide, in hopes of getting one or two that are perfect. Any suggestions would be greatly appreciated. Thanks, Patty Lott UAB CMBD Core Lab 205-934-2007 From joelleweaver <@t> hotmail.com Wed Jan 22 09:02:07 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 22 09:02:14 2014 Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? In-Reply-To: References: Message-ID: don't use Ventana, but I use the Abcam concentrate at 1:100, citrate buffer- HIER and it works great. Usually cell marque AB are good, but had good luck with this one, maybe give it a try? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mrs.abby.maples@gmail.com > Date: Wed, 22 Jan 2014 08:11:11 -0600 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? > > Fellow histotechs, > > We've been running mammoglobin testing on our Ventana Benchmark XT. > However, Cell Marque (our supplier of mammoglobin antibody) has changed the > concentration. Our old concentration was 0.12 mg/mL, the new concentration > is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal. We tried > running the old protocol with the new concentration, but the results were > not adequate (see below). We tried running the protocol given to us by Cell > Marque, but theirs is specific towards the Benchmark UltraView and also > returned inadequate results. > > We process our tissue from formalin to paraffin. Our lab uses the iVew DAB > detection kit. > > Our old protocol: > Deparaffinization > Conditioner #1 (short 8 minute conditioning) > Mild CC1 (mild 30 minutes conditioning) > Standard CC1 (standard 60 minute conditioning) > No enzyme > 37C antibody incubation temperature > No titration > Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes > A/B Block > Hematoxylin counterstain 4 minutes > Blueing post counterstain 4 minutes > > Does anyone else use this product from Ventana/Cell Marque? Does anyone > have a working protocol for a weaker antibody titration? > > Any help would be greatly appreciated! > > *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM* > Histotechnologist, Surgical Pathology > Mercy Medical Center > 701 10th Street SE > Cedar Rapids, IA 52403 > Lab Phone: (319) 398-6827 > Cell: (319) 432-1530 > Fax: (319) 221-8767 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Jan 22 09:04:46 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Jan 22 09:04:51 2014 Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? In-Reply-To: References: Message-ID: also have IVD mammoglobin, clone 31A5 rabbit monoclonal, works really well for me at 1:75- if you need/prefer IVD from cancer diagnostics, the other I posted is ASR. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mrs.abby.maples@gmail.com > Date: Wed, 22 Jan 2014 08:11:11 -0600 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? > > Fellow histotechs, > > We've been running mammoglobin testing on our Ventana Benchmark XT. > However, Cell Marque (our supplier of mammoglobin antibody) has changed the > concentration. Our old concentration was 0.12 mg/mL, the new concentration > is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal. We tried > running the old protocol with the new concentration, but the results were > not adequate (see below). We tried running the protocol given to us by Cell > Marque, but theirs is specific towards the Benchmark UltraView and also > returned inadequate results. > > We process our tissue from formalin to paraffin. Our lab uses the iVew DAB > detection kit. > > Our old protocol: > Deparaffinization > Conditioner #1 (short 8 minute conditioning) > Mild CC1 (mild 30 minutes conditioning) > Standard CC1 (standard 60 minute conditioning) > No enzyme > 37C antibody incubation temperature > No titration > Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes > A/B Block > Hematoxylin counterstain 4 minutes > Blueing post counterstain 4 minutes > > Does anyone else use this product from Ventana/Cell Marque? Does anyone > have a working protocol for a weaker antibody titration? > > Any help would be greatly appreciated! > > *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM* > Histotechnologist, Surgical Pathology > Mercy Medical Center > 701 10th Street SE > Cedar Rapids, IA 52403 > Lab Phone: (319) 398-6827 > Cell: (319) 432-1530 > Fax: (319) 221-8767 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> dbiosys.com Wed Jan 22 09:24:40 2014 From: mike <@t> dbiosys.com (Mike Thompson) Date: Wed Jan 22 09:25:07 2014 Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? Message-ID: Diagnostic Biosystems has Rabbit Monos Website below Mike Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 "IHC Made Affordable" www.dbiosys.com joelle weaver wrote: >also have IVD mammoglobin, clone 31A5 rabbit monoclonal, works really well for me at 1:75- if you need/prefer IVD from cancer diagnostics, the other I posted is ASR. > > > > >Joelle Weaver MAOM, HTL (ASCP) QIHC > >> From: mrs.abby.maples@gmail.com >> Date: Wed, 22 Jan 2014 08:11:11 -0600 >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? >> >> Fellow histotechs, >> >> We've been running mammoglobin testing on our Ventana Benchmark XT. >> However, Cell Marque (our supplier of mammoglobin antibody) has changed the >> concentration. Our old concentration was 0.12 mg/mL, the new concentration >> is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal. We tried >> running the old protocol with the new concentration, but the results were >> not adequate (see below). We tried running the protocol given to us by Cell >> Marque, but theirs is specific towards the Benchmark UltraView and also >> returned inadequate results. >> >> We process our tissue from formalin to paraffin. Our lab uses the iVew DAB >> detection kit. >> >> Our old protocol: >> Deparaffinization >> Conditioner #1 (short 8 minute conditioning) >> Mild CC1 (mild 30 minutes conditioning) >> Standard CC1 (standard 60 minute conditioning) >> No enzyme >> 37C antibody incubation temperature >> No titration >> Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes >> A/B Block >> Hematoxylin counterstain 4 minutes >> Blueing post counterstain 4 minutes >> >> Does anyone else use this product from Ventana/Cell Marque? Does anyone >> have a working protocol for a weaker antibody titration? >> >> Any help would be greatly appreciated! >> >> *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM* >> Histotechnologist, Surgical Pathology >> Mercy Medical Center >> 701 10th Street SE >> Cedar Rapids, IA 52403 >> Lab Phone: (319) 398-6827 >> Cell: (319) 432-1530 >> Fax: (319) 221-8767 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Wed Jan 22 09:27:13 2014 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Wed Jan 22 09:27:17 2014 Subject: [Histonet] zebrafish embryos histology In-Reply-To: <372C4AF089B6AE48B36F064FA891FF78138574ED@UABEXMB3.ad.uab.edu> References: <372C4AF089B6AE48B36F064FA891FF78138574ED@UABEXMB3.ad.uab.edu> Message-ID: Patty, I have personally never performed histology on zebra fish embryo's, but if I was to test it out on my own I might try using the JB4 Plus GMA kit. It just seems to me that paraffin might cause too much shrinkage and maybe using an MMA protocol might be too harsh on the tissue. Besides, given the hydrophilic nature of GMA it just might be the best overall solution. Best, Jack > From: plott@uab.edu > To: histonet@lists.utsouthwestern.edu > Date: Wed, 22 Jan 2014 15:00:40 +0000 > Subject: [Histonet] zebrafish embryos histology > > Can anyone give me a method for zebra-fish embryo histology? The papers I've read show photos, but no description of histology in M & M. I need to put several embryos in each block, and get the orientation correct, and put multiple sections on each slide, in hopes of getting one or two that are perfect. Any suggestions would be greatly appreciated. > Thanks, > Patty Lott > UAB CMBD Core Lab > 205-934-2007 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Wed Jan 22 10:01:14 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Jan 22 10:01:24 2014 Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? In-Reply-To: References: Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276B16BEAF@CVM76.vetmed.wsu.edu> Hi Abby, Since the concentration is about a third of the original, I would try programming the XT to run the primary for 2 hrs instead of 32 min. You may also need to up the secondary time if that isn't sufficient. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abby L. Maples Sent: Wednesday, January 22, 2014 6:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? Fellow histotechs, We've been running mammoglobin testing on our Ventana Benchmark XT. However, Cell Marque (our supplier of mammoglobin antibody) has changed the concentration. Our old concentration was 0.12 mg/mL, the new concentration is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal. We tried running the old protocol with the new concentration, but the results were not adequate (see below). We tried running the protocol given to us by Cell Marque, but theirs is specific towards the Benchmark UltraView and also returned inadequate results. We process our tissue from formalin to paraffin. Our lab uses the iVew DAB detection kit. Our old protocol: Deparaffinization Conditioner #1 (short 8 minute conditioning) Mild CC1 (mild 30 minutes conditioning) Standard CC1 (standard 60 minute conditioning) No enzyme 37C antibody incubation temperature No titration Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes A/B Block Hematoxylin counterstain 4 minutes Blueing post counterstain 4 minutes Does anyone else use this product from Ventana/Cell Marque? Does anyone have a working protocol for a weaker antibody titration? Any help would be greatly appreciated! *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM* Histotechnologist, Surgical Pathology Mercy Medical Center 701 10th Street SE Cedar Rapids, IA 52403 Lab Phone: (319) 398-6827 Cell: (319) 432-1530 Fax: (319) 221-8767 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Jan 22 10:02:24 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jan 22 10:02:39 2014 Subject: [Histonet] zebrafish embryos histology In-Reply-To: <372C4AF089B6AE48B36F064FA891FF78138574ED@UABEXMB3.ad.uab.edu> References: <372C4AF089B6AE48B36F064FA891FF78138574ED@UABEXMB3.ad.uab.edu> Message-ID: <1249144087.13085563.1390406544881.JavaMail.root@comcast.net> Patty, did some zebrafish work years ago, either pure histology or sections after we did whole mount ISH on the embryo's.? As per Jack Ratliff's post, paraffin I found was really tough for orientation and for getting enough sections of such small embryo's.? But would use, as Jack?suggested, JB-4 Plus and would get beautiful sections, many embryo's in a block and multiple sections per slide.? What I would do is under a dissecting scope would, with a VERY fine tool, push the multiple embryo's into an ordered row with similar orientation in the unpolymerized GMA block.? Polymerize.? If I wanted longitudinal sections, cut the block as is.? If we wanted cross-sections, just gross cut the polymerized block and put it in a second GMA block of unpolymerized GMA?and stand it up so the embryo's were on end and polymerize .? Was every individual embryo?correct?? No!? But enough were so got great H&E sections or also seeing the ISH probe revealed at the cellular level. ? Ray, retired in Seattle ----- Original Message ----- From: "Patricia F Lott" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, January 22, 2014 7:00:40 AM Subject: [Histonet] zebrafish embryos histology Can anyone give me a method for zebra-fish embryo histology? ?The papers I've read show photos, but no description of histology in M & M. ?I need to put several embryos in each block, and get the orientation correct, and put multiple sections on each slide, in hopes of getting one or two that are perfect. ?Any suggestions would be greatly appreciated. Thanks, Patty Lott UAB CMBD Core Lab 205-934-2007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Jan 22 10:24:53 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Jan 22 10:25:00 2014 Subject: [Histonet] Re: zebrafish embryos histology Message-ID: <9F3CFEE76E51B64991C7485270890B404982336E@EX5.lj.gnf.org> Hi Patty, We did some of these in paraffin in my other life in Kansas City. Essentially for these we processed them in paraffin, and would embed them in Ls, letting gravity do what it does best. We would line them up side by side, as perpendicular to the edge as we could. We had a stereomicroscope attached to an arm so we could see what we were doing with the small samples, and would use either a toothpick, wooden stick, or very fine brush to orient them in the embedding L. If we needed transverse sections, we would just mount the block as such in a standard specimen clamp and section. Honestly for us it was much quicker than using plastics, and it gave us the ability to do regular specimen and IHC stains on them if needed. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From mrs.abby.maples <@t> gmail.com Wed Jan 22 11:02:25 2014 From: mrs.abby.maples <@t> gmail.com (Abby L. Maples) Date: Wed Jan 22 11:02:53 2014 Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? In-Reply-To: References: Message-ID: Thank you everyone! You've all been so helpful regarding the mammaglobin. Ventana has called me and I think we got things figured out (at least tenatively) and the wheels are in motion. If it still doesn't work, our rep will come out and see us. Thanks again for your input! Great ideas abound!! On Wed, Jan 22, 2014 at 10:44 AM, Thomas Jasper wrote: > Hi Abby, > > I have a couple of suggestions - > > 1) Try to extend your incubation times. You may need to do a couple-three > of these to get a decent result. > > 2) Contact Ventana and have a field specialist come out and help you with > working out this problem on the Benchmark. My experiences with Ventana > have been positive in this regard and I've always found them willing to > help in any way possible. > > You may have already thought of, and done these things, I don't know. > Just thought I'd mention them. > > Good luck to you, > Tom Jasper > > > Thomas Jasper HT (ASCP) BAS > AP/CP Supervisor > Deaconess Hospital > 600 Mary Street > Evansville, IN 47747 > Thomas.jasper@deaconess.com > 812-450-2485 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abby L. Maples > Sent: Wednesday, January 22, 2014 8:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Marque Mammoglobin (31A5) New Protocol? > > Fellow histotechs, > > We've been running mammoglobin testing on our Ventana Benchmark XT. > However, Cell Marque (our supplier of mammoglobin antibody) has changed > the concentration. Our old concentration was 0.12 mg/mL, the new > concentration is weaker at 0.04 mg/mL. The antibody is a rabbit monoclonal. > We tried running the old protocol with the new concentration, but the > results were not adequate (see below). We tried running the protocol given > to us by Cell Marque, but theirs is specific towards the Benchmark > UltraView and also returned inadequate results. > > We process our tissue from formalin to paraffin. Our lab uses the iVew DAB > detection kit. > > Our old protocol: > Deparaffinization > Conditioner #1 (short 8 minute conditioning) Mild CC1 (mild 30 minutes > conditioning) Standard CC1 (standard 60 minute conditioning) No enzyme 37C > antibody incubation temperature No titration > Antibody: Mammoglobin 31A5 at 0.12 mg/mL concentration for 32 minutes A/B > Block Hematoxylin counterstain 4 minutes Blueing post counterstain 4 minutes > > Does anyone else use this product from Ventana/Cell Marque? Does anyone > have a working protocol for a weaker antibody titration? > > Any help would be greatly appreciated! > > *Abby L. H. Maples, BS, MPH, HTL (ASCP)CM* Histotechnologist, Surgical > Pathology Mercy Medical Center > 701 10th Street SE > Cedar Rapids, IA 52403 > Lab Phone: (319) 398-6827 > Cell: (319) 432-1530 > Fax: (319) 221-8767 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From koellingr <@t> comcast.net Wed Jan 22 12:14:00 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jan 22 12:14:09 2014 Subject: [Histonet] zebrafish embryos histology In-Reply-To: <1249144087.13085563.1390406544881.JavaMail.root@comcast.net> References: <372C4AF089B6AE48B36F064FA891FF78138574ED@UABEXMB3.ad.uab.edu> <1249144087.13085563.1390406544881.JavaMail.root@comcast.net> Message-ID: <556310965.13156342.1390414440386.JavaMail.root@comcast.net> Hi Patty, You sort of piqued my curiosity of what is going on in the zebra fish world and found this youtube, 6 minute film? http://www.youtube.com/watch?v=kZDwo20hl1E&feature=youtu.be About what is going on at Welcome Trust Research on zebra fish but maybe you know all this already.? Anyway, I think the film is pretty neat with some incredible (not histology) but CONFOCAL slices through the fish.? We had success doing this by confocal?on whole-mount but again when studying gene manipulation and cell signal distribution and wanting histology in embryo's, had better luck with GMA.? Paraffin as mentioned can certainly be done and ?is certainly easier but we could never get the resolution or number of sections?we desired using paraffin.? Maybe the people in lab from this film can give you some suggestions. ? Ray, retired in Seattle ----- Original Message ----- From: koellingr@comcast.net To: "Patricia F Lott" Cc: histonet@lists.utsouthwestern.edu Sent: Wednesday, January 22, 2014 8:02:24 AM Subject: Re: [Histonet] zebrafish embryos histology Patty, did some zebrafish work years ago, either pure histology or sections after we did whole mount ISH on the embryo's.? As per Jack Ratliff's post, paraffin I found was really tough for orientation and for getting enough sections of such small embryo's.? But would use, as Jack?suggested, JB-4 Plus and would get beautiful sections, many embryo's in a block and multiple sections per slide.? What I would do is under a dissecting scope would, with a VERY fine tool, push the multiple embryo's into an ordered row with similar orientation in the unpolymerized GMA block.? Polymerize.? If I wanted longitudinal sections, cut the block as is.? If we wanted cross-sections, just gross cut the polymerized block and put it in a second GMA block of unpolymerized GMA?and stand it up so the embryo's were on end and polymerize .? Was every individual embryo?correct?? No!? But enough were so got great H&E sections or also seeing the ISH probe revealed at the cellular level. ? Ray, retired in Seattle ----- Original Message ----- From: "Patricia F Lott" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, January 22, 2014 7:00:40 AM Subject: [Histonet] zebrafish embryos histology Can anyone give me a method for zebra-fish embryo histology? ?The papers I've read show photos, but no description of histology in M & M. ?I need to put several embryos in each block, and get the orientation correct, and put multiple sections on each slide, in hopes of getting one or two that are perfect. ?Any suggestions would be greatly appreciated. Thanks, Patty Lott UAB CMBD Core Lab 205-934-2007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bgambic1 <@t> jhmi.edu Wed Jan 22 12:26:03 2014 From: Bgambic1 <@t> jhmi.edu (Bonnie Gambichler) Date: Wed Jan 22 12:26:11 2014 Subject: [Histonet] RE: Zebra-fish embryo histology Message-ID: <5099B930031AAA4588AB898A54692211448AD6CF@ONCEXNODE4.win.ad.jhu.edu> Patty, Our lab has processed zebra fish embryos for paraffin sectioning. The embryos were fixed overnight in 4% paraformaldehyde and processed on a biopsy program without any heat. The key is to pop the swim bladders of the fish so that they will lay flat during embedding. We put multiple pieces into each biopsy sized mold and were able to get a good section with intestine on at least one of the fish in each block. It is imperative that you soak the blocks, on a paper towel moistened with diluted ammonium hydroxide, on ice for at least an hour to get decent sections because the tissue is very dry. Best Regards, Bonnie Gambichler, HT ( ASCP ) JHU Oncology Tissue Services Lab From rhworkman <@t> uro.com Wed Jan 22 12:53:51 2014 From: rhworkman <@t> uro.com (Renee H. Workman) Date: Wed Jan 22 12:53:57 2014 Subject: [Histonet] Retaining unstained glass slides Message-ID: <0137e3eac0d8430aabb2c777998b6c6b@CO1PR05MB316.namprd05.prod.outlook.com> How long due most labs retain glass slides? Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. From wbenton <@t> cua.md Wed Jan 22 13:05:41 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Jan 22 13:10:31 2014 Subject: [Histonet] RE: Retaining unstained glass slides In-Reply-To: <0137e3eac0d8430aabb2c777998b6c6b@CO1PR05MB316.namprd05.prod.outlook.com> References: <0137e3eac0d8430aabb2c777998b6c6b@CO1PR05MB316.namprd05.prod.outlook.com> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF2CF@CUAEXH1.GCU-MD.local> Follow the guidelines of your accrediting agency. CAP = 10 years minimum ANP.12500 Record Retention Phase II Surgical pathology records and materials are retained for an appropriate period. NOTE 1: Minimum requirements for surgical pathology, providing these are not less stringent than local, state and national regulations, are: 1. Accession log records - 2 years 2. Wet tissue (stock bottle) - 2 weeks after final report 3. Paraffin blocks - 10 years (subject to Note 2, below) 4. Glass slides (including control slides) and reports - 10 years (slides must remain readable for this period) 5. Surgical pathology reports - 10 years (see Notes 3 and 4, below) 6. Fluorochrome-stained slides - at the discretion of the laboratory director 7. Fine needle aspiration slides - 10 years 8. Images of FISH studies - 10 years (see Note 5, below) Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman [rhworkman@uro.com] Sent: Wednesday, January 22, 2014 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retaining unstained glass slides How long due most labs retain glass slides? Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From ncosenza <@t> siumed.edu Wed Jan 22 13:43:32 2014 From: ncosenza <@t> siumed.edu (Nicole Cosenza) Date: Wed Jan 22 13:43:36 2014 Subject: [Histonet] lyophilizing company Message-ID: <52E01F64.20801@siumed.edu> Histonetters: My lab is needing to lyophilize some skin tissue. Is there a company that offers such services? From lcolbert <@t> pathmdlabs.com Wed Jan 22 15:21:03 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Jan 22 15:23:04 2014 Subject: [Histonet] Buffer pH for IHC Message-ID: <12ECD7346266D74691EC2BFC75285E452F3F869F@BFL323E10.pathmdlabs.local> What does everyone use to test the pH of their buffers for IHC? Are pH strips adequate or do you have to have an actual pH meter? If you use a meter, any suggestions on a make and model? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From shive003 <@t> umn.edu Wed Jan 22 16:07:36 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Jan 22 16:07:44 2014 Subject: [Histonet] Buffer pH for IHC In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F3F869F@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F3F869F@BFL323E10.pathmdlabs.local> Message-ID: pH meter; Corning. On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert wrote: > What does everyone use to test the pH of their buffers for IHC? Are pH > strips adequate or do you have to have an actual pH meter? If you use a > meter, any suggestions on a make and model? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and > confidential information, including patient information protected by > federal and state privacy laws. It is intended only for the use of the > person(s) named above. If you are not the intended recipient, you are > hereby notified that any review, dissemination, distribution, or > duplication of this communication is strictly prohibited. If you are not > the intended recipient, please contact the sender by reply email and > destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From sidsjoshi07 <@t> gmail.com Wed Jan 22 22:48:40 2014 From: sidsjoshi07 <@t> gmail.com (sid sharma) Date: Wed Jan 22 22:48:46 2014 Subject: [Histonet] How to do validation of PLA2R antibody on serum Message-ID: From ree3 <@t> leicester.ac.uk Thu Jan 23 02:52:04 2014 From: ree3 <@t> leicester.ac.uk (Edwards, Richard) Date: Thu Jan 23 02:52:33 2014 Subject: [Histonet] Which] Buffer for IHC Message-ID: And what is the buffer of choice, PBS, TBS, with or without protein/detergents or whatever, thanks. Richard Edwards Leicester University U.K. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: 22 January 2014 22:08 To: Laurie Colbert Cc: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Buffer pH for IHC pH meter; Corning. On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert wrote: > What does everyone use to test the pH of their buffers for IHC? Are > pH strips adequate or do you have to have an actual pH meter? If you > use a meter, any suggestions on a make and model? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged > and confidential information, including patient information protected > by federal and state privacy laws. It is intended only for the use of > the > person(s) named above. If you are not the intended recipient, you are > hereby notified that any review, dissemination, distribution, or > duplication of this communication is strictly prohibited. If you are > not the intended recipient, please contact the sender by reply email > and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Thu Jan 23 10:09:11 2014 From: azdudley <@t> hotmail.com (anita) Date: Thu Jan 23 10:09:16 2014 Subject: [Histonet] Lysol I.C. Message-ID: We are using Lysol I.C. for disinfecting the ventana benchmark. I thought I purchased it from Cardinal but I'm not sure now where we got it. Are others using this for their machine and where are you purchasing it from? Thanks so much. Anita Dudley Providence Hosp. Mobile, Al. 36695 From wbenton <@t> cua.md Thu Jan 23 10:20:08 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Jan 23 10:20:30 2014 Subject: [Histonet] Lysol I.C. In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF2DE@CUAEXH1.GCU-MD.local> Lab Safety Supply sells it as well. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita [azdudley@hotmail.com] Sent: Thursday, January 23, 2014 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lysol I.C. We are using Lysol I.C. for disinfecting the ventana benchmark. I thought I purchased it from Cardinal but I'm not sure now where we got it. Are others using this for their machine and where are you purchasing it from? Thanks so much. Anita Dudley Providence Hosp. Mobile, Al. 36695 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Nancy_Schmitt <@t> pa-ucl.com Thu Jan 23 10:35:55 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Jan 23 10:36:00 2014 Subject: [Histonet] p16 Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36815578C5@PEITHA.wad.pa-ucl.com> Good Morning! Where are you currently obtaining p16 from? Thanks a bunch! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Please visit our website: http://www.uclaccess.com/ NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From LSebree <@t> uwhealth.org Thu Jan 23 10:37:23 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Jan 23 10:37:28 2014 Subject: [Histonet] Lysol I.C. In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF095ED9@UWHC-MBX12.uwhis.hosp.wisc.edu> That's what we use Anita and we purchase it from Cardinal as well. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita Sent: Thursday, January 23, 2014 10:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lysol I.C. We are using Lysol I.C. for disinfecting the ventana benchmark. I thought I purchased it from Cardinal but I'm not sure now where we got it. Are others using this for their machine and where are you purchasing it from? Thanks so much. Anita Dudley Providence Hosp. Mobile, Al. 36695 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Thu Jan 23 10:40:51 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Jan 23 10:41:39 2014 Subject: [Histonet] RE: p16 In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36815578C5@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36815578C5@PEITHA.wad.pa-ucl.com> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF2E0@CUAEXH1.GCU-MD.local> I believe Ventana owns the rights to that antibody now. It was previously owned by MTM Labs. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt [Nancy_Schmitt@pa-ucl.com] Sent: Thursday, January 23, 2014 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Good Morning! Where are you currently obtaining p16 from? Thanks a bunch! Nancy Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Please visit our website: http://www.uclaccess.com/ NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From shive003 <@t> umn.edu Thu Jan 23 10:45:26 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jan 23 10:45:31 2014 Subject: [Histonet] Re: Which] Buffer for IHC In-Reply-To: References: Message-ID: To calibrate a pH meter, we use purchased pH buffers at pH 4.0, 7.0, and 10.0. To perform the IHC method, we use TBS (pH 7.6) with Tween 20 as our rinse buffer. Jan Shivers On Thu, Jan 23, 2014 at 2:52 AM, Edwards, Richard wrote: > And what is the buffer of choice, PBS, TBS, with or without > protein/detergents or whatever, thanks. > > > Richard > Edwards > > > Leicester University > > > > U.K. > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > Sent: 22 January 2014 22:08 > To: Laurie Colbert > Cc: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: Re: [Histonet] Buffer pH for IHC > > pH meter; Corning. > > > On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert >wrote: > > > What does everyone use to test the pH of their buffers for IHC? Are > > pH strips adequate or do you have to have an actual pH meter? If you > > use a meter, any suggestions on a make and model? > > > > Laurie Colbert, HT (ASCP) > > Histology Supervisor > > PATH MD > > 8158 Beverly Blvd. > > Los Angeles, CA 90048 > > (323) 648-3214 direct > > (424) 245-7284 main lab > > > > The information contained in this transmission may contain privileged > > and confidential information, including patient information protected > > by federal and state privacy laws. It is intended only for the use of > > the > > person(s) named above. If you are not the intended recipient, you are > > hereby notified that any review, dissemination, distribution, or > > duplication of this communication is strictly prohibited. If you are > > not the intended recipient, please contact the sender by reply email > > and destroy all copies of the original message. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu From esarricks <@t> gmail.com Thu Jan 23 10:49:15 2014 From: esarricks <@t> gmail.com (Erin Sarricks) Date: Thu Jan 23 10:49:26 2014 Subject: [Histonet] IHC-start-up Message-ID: <0BBCDDC9-0282-43A9-BA62-BFC32968D283@gmail.com> Hi all- Our histopathology lab is looking to set up an IHC component to service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! Regards, Erin Sarricks, HT (ASCP) From chapcl <@t> yahoo.com Thu Jan 23 11:44:58 2014 From: chapcl <@t> yahoo.com (Will Chappell) Date: Thu Jan 23 11:45:07 2014 Subject: [Histonet] IHC-start-up In-Reply-To: <0BBCDDC9-0282-43A9-BA62-BFC32968D283@gmail.com> References: <0BBCDDC9-0282-43A9-BA62-BFC32968D283@gmail.com> Message-ID: <172FEFCD-C0E4-4894-A26B-FFD15F6D6E9E@yahoo.com> Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. I would steer clear of any platforms that do not let you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. William Chappell Sent from my iPhone > On Jan 23, 2014, at 11:49 AM, Erin Sarricks wrote: > > Hi all- > > Our histopathology lab is looking to > set up an IHC component to service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! > > Regards, > > Erin Sarricks, HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Thu Jan 23 12:03:13 2014 From: cforster <@t> umn.edu (Colleen Forster) Date: Thu Jan 23 12:03:27 2014 Subject: [Histonet] IHC-start-up In-Reply-To: <172FEFCD-C0E4-4894-A26B-FFD15F6D6E9E@yahoo.com> References: <0BBCDDC9-0282-43A9-BA62-BFC32968D283@gmail.com> <172FEFCD-C0E4-4894-A26B-FFD15F6D6E9E@yahoo.com> Message-ID: <52E15961.5070709@umn.edu> I totally agree with William. I do animal IHC and I am, using the Biocare Nemesis, which be the same as the Dako platform. Completely open so you can do what you need to at a cost you can afford Colleen Forster HT(ASCP)QIHC u of MN On 1/23/2014 11:44 AM, Will Chappell wrote: > Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. > > I would steer clear of any platforms that do not let you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. > > William Chappell > > Sent from my iPhone > >> On Jan 23, 2014, at 11:49 AM, Erin Sarricks wrote: >> >> Hi all- >> >> Our histopathology lab is looking to >> set up an IHC component to service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! >> >> Regards, >> >> Erin Sarricks, HT (ASCP) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> dbiosys.com Thu Jan 23 12:09:59 2014 From: mike <@t> dbiosys.com (Mike Thompson) Date: Thu Jan 23 12:10:28 2014 Subject: [Histonet] IHC-start-up Message-ID: Diagnostic Biosystems has the same platform with tech support that is outstanding! Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 "IHC Made Affordable" www.dbiosys.com Colleen Forster wrote: >I totally agree with William. I do animal IHC and I am, using the >Biocare Nemesis, which be the same as the Dako platform. Completely open >so you can do what you need to at a cost you can afford > >Colleen Forster HT(ASCP)QIHC >u of MN > > > >On 1/23/2014 11:44 AM, Will Chappell wrote: >> Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. >> >> I would steer clear of any platforms that do not let you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. >> >> William Chappell >> >> Sent from my iPhone >> >>> On Jan 23, 2014, at 11:49 AM, Erin Sarricks wrote: >>> >>> Hi all- >>> >>> Our histopathology lab is looking to >>> set up an IHC component to service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! >>> >>> Regards, >>> >>> Erin Sarricks, HT (ASCP) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Thu Jan 23 12:12:28 2014 From: JWatson <@t> gnf.org (James Watson) Date: Thu Jan 23 12:12:38 2014 Subject: [Histonet] IHC-start-up In-Reply-To: <172FEFCD-C0E4-4894-A26B-FFD15F6D6E9E@yahoo.com> References: <0BBCDDC9-0282-43A9-BA62-BFC32968D283@gmail.com> <172FEFCD-C0E4-4894-A26B-FFD15F6D6E9E@yahoo.com> Message-ID: Erin, We run the Ventana Discovery XT at our lab and average 15,000 antibodies stained a year using 5 stainers on animal tissue. If you are working on volume I would also look at the Leica Bond system. Leica and Ventana both have released or are releasing more flexible systems as far as programing staining protocols. With the Ventanas you are tied into their chromogen labeling systems and LCS, but most other reagents can be substituted. Other systems are more open, but do not offer on line deparaffinization or HEIR. These would be great if not working with large volume. The Ventanas have worked great for us, but like all stainers they do have their little idiosyncrasies. Look at what fits your needs and look closely at the cost of running a system vs. efficiency. Jamie James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will Chappell Sent: Thursday, January 23, 2014 9:45 AM To: Erin Sarricks Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC-start-up Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. I would steer clear of any platforms that do not let you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. William Chappell Sent from my iPhone > On Jan 23, 2014, at 11:49 AM, Erin Sarricks wrote: > > Hi all- > > Our histopathology lab is looking to > set up an IHC component to service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! > > Regards, > > Erin Sarricks, HT (ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thoward <@t> unm.edu Thu Jan 23 12:26:29 2014 From: thoward <@t> unm.edu (Tamara Howard) Date: Thu Jan 23 12:26:36 2014 Subject: [Histonet] RE: Buffer pH for IHC Message-ID: <64fc6809d9f64277bf28f996ccbf4656@DM2PR07MB382.namprd07.prod.outlook.com> Keep in mind that not all pH electrodes are Tris-sensitive; check the specs on the electrode you plan to buy if you use Tris buffers. ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM From catherinesimonson <@t> gmail.com Thu Jan 23 12:45:23 2014 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Thu Jan 23 12:45:26 2014 Subject: [Histonet] How to process tissues submitted in acrylamide Message-ID: Hello Helpful Histonetters, I have some samples that were fixed in PFA, placed in acrylamide gel and then treated with iodine for microCT at another facility. The investigator now wants to follow up and get H&E's on these samples. I know how to deal with the iodine, but my question is this: what is the best procedure to process these samples to slide? Do I run on a long processing schedule on the processor, or do I do sucrose cryoprotection and get frozen. Morphometry seems to be an important factor. Thanks in advance, Catherine Simonson, B.S., HT(ASCP) From shive003 <@t> umn.edu Thu Jan 23 12:56:45 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jan 23 12:56:56 2014 Subject: [Histonet] IHC-start-up In-Reply-To: References: <0BBCDDC9-0282-43A9-BA62-BFC32968D283@gmail.com> <172FEFCD-C0E4-4894-A26B-FFD15F6D6E9E@yahoo.com> Message-ID: I work in animal tissue only. I also agree that an open autostainer system works best, since you may have to run multiple dilutions and incubation times for the same antibody because of the different species' reactivities. You may also need multiple types of reagents to cover detection in different species, so you'll need a system which will allow you to use multiple vendor products. An aside... The CD4 and CD8 Abs that I've done on various animals are usually only done on frozen sections, so I tend to perform that IHC manually, in order to be able to rinse slides more gently than an autostainer does (and do overnight incubation with primary Ab, if necessary). Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu On Thu, Jan 23, 2014 at 12:12 PM, James Watson wrote: > Erin, > > We run the Ventana Discovery XT at our lab and average 15,000 antibodies > stained a year using 5 stainers on animal tissue. If you are working on > volume I would also look at the Leica Bond system. Leica and Ventana > both have released or are releasing more flexible systems as far as > programing staining protocols. With the Ventanas you are tied into their > chromogen labeling systems and LCS, but most other reagents can be > substituted. > > Other systems are more open, but do not offer on line deparaffinization > or HEIR. These would be great if not working with large volume. > > The Ventanas have worked great for us, but like all stainers they do have > their little idiosyncrasies. > > Look at what fits your needs and look closely at the cost of running a > system vs. efficiency. > > Jamie > > James Watson HT ASCP > GNF Genomics Institute of the Novartis Research Foundation > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Will Chappell > Sent: Thursday, January 23, 2014 9:45 AM > To: Erin Sarricks > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC-start-up > > Doing ihc on animal tissue is very tricky, if for no other reason than you > are trying to standardize an antibody across multiple species. You will > need to use every trick in the book and invent some of your own to get > reproducible results. > > I would steer clear of any platforms that do not let you customize > everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have > their quirks but they have the openness you require. > > William Chappell > > Sent from my iPhone > > > On Jan 23, 2014, at 11:49 AM, Erin Sarricks wrote: > > > > Hi all- > > > > Our histopathology lab is looking to > > set up an IHC component to service the request of our clients. All work > is on animal tissue. We will probably run about 1,000 IHC slides the first > year and hope to increase the workload each year. Some stains we would > run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana > Discovery XT and appeared to be a good fit for our needs. Does anyone have > any other advice on other machines we should look into? Any information or > advice would be greatly appreciated. Thank you! > > > > Regards, > > > > Erin Sarricks, HT (ASCP) > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From akbitting <@t> geisinger.edu Thu Jan 23 13:21:10 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Jan 23 13:21:22 2014 Subject: [Histonet] IHC-start-up In-Reply-To: References: Message-ID: <77F52EFAB8B1694B885E277C48FCD0F662AC0C39@GHSEXMBX1W8K1V.geisinger.edu> So Mike, It appears you are a sales director for the company, which makes your opinion null and void. Sorry. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Thompson Sent: Thursday, January 23, 2014 1:10 PM To: Colleen Forster Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC-start-up Diagnostic Biosystems has the same platform with tech support that is outstanding! Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 "IHC Made Affordable" www.dbiosys.com Colleen Forster wrote: >I totally agree with William. I do animal IHC and I am, using the >Biocare Nemesis, which be the same as the Dako platform. Completely >open so you can do what you need to at a cost you can afford > >Colleen Forster HT(ASCP)QIHC >u of MN > > > >On 1/23/2014 11:44 AM, Will Chappell wrote: >> Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. >> >> I would steer clear of any platforms that do not let you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. >> >> William Chappell >> >> Sent from my iPhone >> >>> On Jan 23, 2014, at 11:49 AM, Erin Sarricks wrote: >>> >>> Hi all- >>> >>> Our histopathology lab is looking to set up an IHC component to >>> service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! >>> >>> Regards, >>> >>> Erin Sarricks, HT (ASCP) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From tbraud <@t> holyredeemer.com Thu Jan 23 14:01:57 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 23 14:02:00 2014 Subject: [Histonet] RE: IHC In-Reply-To: <20140123172409.9D43E1E8040@trendmess-svr.holyredeemer.local> References: <20140123172409.9D43E1E8040@trendmess-svr.holyredeemer.local> Message-ID: I've used many different platforms and have liked most. Currently we are using BioCare. We LOVE our BioCare Intellipath FLX for our IHC platform. It is very cost effective, completely open, and has very user friendly software. BioCare has been a super company to work with. Fabulous customer support with a very knowledgeable technical staff. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 16 Date: Thu, 23 Jan 2014 11:49:15 -0500 From: Erin Sarricks Subject: [Histonet] IHC-start-up Hi all- Our histopathology lab is looking to set up an IHC component to service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! Regards, Erin Sarricks, HT (ASCP) ***************************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From BergerR1 <@t> email.chop.edu Thu Jan 23 14:18:33 2014 From: BergerR1 <@t> email.chop.edu (Berger, Rebecca) Date: Thu Jan 23 14:18:39 2014 Subject: [Histonet] Wright Stain on paraffin sections Message-ID: <5A590EB108038B4FA01F8CDB39C6541B01308DCD@EXCMBXPW7.chop.edu> Hi all, I am trying to look at eosinophils and neutrophils in FFPE muscle sections. Does anyone have experience with the Wright stain or a Wright staining kit on these types of sections? Or possibly a suggestion for a different protocol to visualize these cells? Thanks! Becky From rhworkman <@t> uro.com Thu Jan 23 14:30:29 2014 From: rhworkman <@t> uro.com (Renee H. Workman) Date: Thu Jan 23 14:30:39 2014 Subject: [Histonet] (no subject) Message-ID: <9540be5ddf054e8b879d45db01cfed52@CO1PR05MB316.namprd05.prod.outlook.com> Sorry everyone, how long due most labs retain unstained glass slides? I have the CAP requirement but due they have requirements for unstained glass slides. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. From lblazek <@t> digestivespecialists.com Thu Jan 23 14:55:03 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jan 23 14:55:07 2014 Subject: [Histonet] RE: IHC In-Reply-To: References: <20140123172409.9D43E1E8040@trendmess-svr.holyredeemer.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E39167F9400D9@IBMB7Exchange.digestivespecialists.com> I second this! Since I've not worked with animal tissue I didn't know quite how to respond to the question but in regards to flexibility, ease of use and unquestionably remarkable support I would not hesitate to check into the Intellipath FLX. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 23, 2014 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC I've used many different platforms and have liked most. Currently we are using BioCare. We LOVE our BioCare Intellipath FLX for our IHC platform. It is very cost effective, completely open, and has very user friendly software. BioCare has been a super company to work with. Fabulous customer support with a very knowledgeable technical staff. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 16 Date: Thu, 23 Jan 2014 11:49:15 -0500 From: Erin Sarricks Subject: [Histonet] IHC-start-up Hi all- Our histopathology lab is looking to set up an IHC component to service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! Regards, Erin Sarricks, HT (ASCP) ***************************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jerrysedgewick <@t> gmail.com Thu Jan 23 15:51:40 2014 From: jerrysedgewick <@t> gmail.com (jerry sedgewick) Date: Thu Jan 23 15:51:53 2014 Subject: [Histonet] Silver in Nucleolus In-Reply-To: <64fc6809d9f64277bf28f996ccbf4656@DM2PR07MB382.namprd07.prod.outlook.com> References: <64fc6809d9f64277bf28f996ccbf4656@DM2PR07MB382.namprd07.prod.outlook.com> Message-ID: <52E18EEC.4060500@gmail.com> I'm working with a lab that stained cat gonads with Warthin Starry. We're looking for Bartonella bacteria. In the first batch that was stained, some nucleii filled with the silver stain, something I've see in published images. In the second batch it appears that nucleolus filled with silver. I haven't seen that in google searches, etc. I wondered if others have seen the nucleolus fill with silver (or is this bartonella?). I'm also wondering how to prevent this sort of thing with proper bench procedures. Thanks! Jerry Sedgewick From rjbuesa <@t> yahoo.com Thu Jan 23 15:55:49 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 23 15:55:53 2014 Subject: [Histonet] Wright Stain on paraffin sections In-Reply-To: <5A590EB108038B4FA01F8CDB39C6541B01308DCD@EXCMBXPW7.chop.edu> References: <5A590EB108038B4FA01F8CDB39C6541B01308DCD@EXCMBXPW7.chop.edu> Message-ID: <1390514149.65884.YahooMailNeo@web120405.mail.ne1.yahoo.com> Becky: You can use Giemsa for tissue sections. under separate cover I am sending my article on the subject. Ren? J. ________________________________ From: "Berger, Rebecca" To: "histonet@lists.utsouthwestern.edu" Sent: Thursday, January 23, 2014 3:18 PM Subject: [Histonet] Wright Stain on paraffin sections Hi all, I am trying to look at eosinophils and neutrophils in FFPE muscle sections. Does anyone have experience with the Wright stain or a Wright staining kit on these types of sections? Or possibly a suggestion for a different protocol to visualize these cells? Thanks! Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Jan 23 18:05:28 2014 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Thu Jan 23 18:05:32 2014 Subject: [Histonet] IHC-start-up In-Reply-To: References: Message-ID: <00b601cf1897$fde49ca0$f9add5e0$@ihctech.net> We use the Leica platforms and just got a Leica RX which is for animal research and ISH, we love the openness of it. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Thompson Sent: Thursday, January 23, 2014 11:10 AM To: Colleen Forster Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC-start-up Diagnostic Biosystems has the same platform with tech support that is outstanding! Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 "IHC Made Affordable" www.dbiosys.com Colleen Forster wrote: >I totally agree with William. I do animal IHC and I am, using the >Biocare Nemesis, which be the same as the Dako platform. Completely >open so you can do what you need to at a cost you can afford > >Colleen Forster HT(ASCP)QIHC >u of MN > > > >On 1/23/2014 11:44 AM, Will Chappell wrote: >> Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. >> >> I would steer clear of any platforms that do not let you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. >> >> William Chappell >> >> Sent from my iPhone >> >>> On Jan 23, 2014, at 11:49 AM, Erin Sarricks wrote: >>> >>> Hi all- >>> >>> Our histopathology lab is looking to set up an IHC component to >>> service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! >>> >>> Regards, >>> >>> Erin Sarricks, HT (ASCP) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Jan 23 18:21:41 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Jan 23 18:22:08 2014 Subject: [Histonet] Silver in Nucleolus In-Reply-To: <52E18EEC.4060500@gmail.com> References: <64fc6809d9f64277bf28f996ccbf4656@DM2PR07MB382.namprd07.prod.outlook.com> <52E18EEC.4060500@gmail.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00A9F6A@xmdb04.nch.kids> Hi Jerry, Are you sure they are not AgNOR's: Lindner, L. E. (1993). Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR). Journal of Histochemistry & Cytochemistry, 41(3), 439-445. Thiebaut, F., Rigaut, J. P., & Reith, A. (1984). Improvement in the Specificity of the Silver Staining Technique for Ag NOR-ASSOCIATED Acidic Proteins in Paraffin Sections. Biotechnic & Histochemistry, 59(3), 181-185. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jerry sedgewick Sent: Friday, 24 January 2014 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Silver in Nucleolus I'm working with a lab that stained cat gonads with Warthin Starry. We're looking for Bartonella bacteria. In the first batch that was stained, some nucleii filled with the silver stain, something I've see in published images. In the second batch it appears that nucleolus filled with silver. I haven't seen that in google searches, etc. I wondered if others have seen the nucleolus fill with silver (or is this bartonella?). I'm also wondering how to prevent this sort of thing with proper bench procedures. Thanks! Jerry Sedgewick _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From ree3 <@t> leicester.ac.uk Fri Jan 24 02:41:56 2014 From: ree3 <@t> leicester.ac.uk (Edwards, Richard) Date: Fri Jan 24 02:43:02 2014 Subject: [Histonet] IHC-start-up In-Reply-To: <0BBCDDC9-0282-43A9-BA62-BFC32968D283@gmail.com> References: <0BBCDDC9-0282-43A9-BA62-BFC32968D283@gmail.com> Message-ID: Your biggest problem is not your choice of machine but which antibodies will work/cross react on animal tissues, many if not most, do not. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erin Sarricks Sent: 23 January 2014 16:49 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC-start-up Hi all- Our histopathology lab is looking to set up an IHC component to service the request of our clients. All work is on animal tissue. We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year. Some stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs. Does anyone have any other advice on other machines we should look into? Any information or advice would be greatly appreciated. Thank you! Regards, Erin Sarricks, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.auge <@t> gmail.com Fri Jan 24 05:58:13 2014 From: tony.auge <@t> gmail.com (Tony Auge) Date: Fri Jan 24 05:58:19 2014 Subject: [Histonet] p16 In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36815578C5@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C36815578C5@PEITHA.wad.pa-ucl.com> Message-ID: I have had excellent results with P16 (JC8) from Santa Cruz Biotech. http://www.scbt.com/datasheet-56330-p16-jc8-antibody.html Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com From mtighe <@t> trudeauinstitute.org Fri Jan 24 09:51:00 2014 From: mtighe <@t> trudeauinstitute.org (Mike Tighe) Date: Fri Jan 24 09:51:07 2014 Subject: [Histonet] Activated carbon Message-ID: <1a898d2554d443edaa3c2a9e271fa6c2@CO2PR07MB476.namprd07.prod.outlook.com> I am looking for a low cost source for activated carbon. So far I am looking at $50 per pound for 6-14 mesh. This is for a VIP 300. Anybody use a different mesh size? Thanks! Mike From akbitting <@t> geisinger.edu Fri Jan 24 10:09:14 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Fri Jan 24 10:09:20 2014 Subject: [Histonet] RE: Activated carbon In-Reply-To: <1a898d2554d443edaa3c2a9e271fa6c2@CO2PR07MB476.namprd07.prod.outlook.com> References: <1a898d2554d443edaa3c2a9e271fa6c2@CO2PR07MB476.namprd07.prod.outlook.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F662AC0F5D@GHSEXMBX1W8K1V.geisinger.edu> www.drsfostersmith.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Friday, January 24, 2014 10:51 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Activated carbon I am looking for a low cost source for activated carbon. So far I am looking at $50 per pound for 6-14 mesh. This is for a VIP 300. Anybody use a different mesh size? Thanks! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From sforeman <@t> labpath.com Fri Jan 24 10:17:18 2014 From: sforeman <@t> labpath.com (Susan Foreman) Date: Fri Jan 24 10:19:45 2014 Subject: [Histonet] HPV-ISH Probes for Ventana Benchmark Ultra Message-ID: <000c01cf191f$c0bf3230$423d9690$@com> Has anyone worked up HPV-ISH testing on the Ventana Benchmark Ultra using probes other than those previously available through Ventana? It's been several months since I checked on this topic. What labs still have Family 6 and Family 16 Probe left and still offering the testing? Many Thanks, Susan 865.584.1933 From amosbrooks <@t> gmail.com Fri Jan 24 11:09:09 2014 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Jan 24 11:09:15 2014 Subject: [Histonet] Mouse GranzymeB Message-ID: Ok Musketeers, I am trying to detect cytotoxic T-Cells in formalin fixed paraffin embedded mouse liver. GranzymeB should do the trick. I have a rabbit anti mouse (and human and rat) GranzymeB from abCam (ab53097). The spleen that I ran as a control with it works fine. Nice T-cells and no signifigant background staining. The liver on the others hand has some lymphocyte staining, presumably cytotoxic T-cells as expected, but it looks like every hepatocyte nuclei are also picking it up. Grrr, right? If this antibody had been raised in mouse, I wouldn't be surprised. Also, if the spleen looked similar, I wouldn't be surprised. Do any of you have any ideas ab pi ut what this might be? Alternatively, does anyone have any ideas about another antibody that would work here? CD4 or CD8 would be great here, but its FFPE mouse, so sadly that's out (more grumbling). For the completionists out there, I used EDTA pH9, peroxide block and detected the antibody (diluted 1:800) with Envision (rabbit) and DAB from Dako. Any suggestions would be vastly appreciated, Happy Friday, Amos From marielachertoff <@t> gmail.com Fri Jan 24 13:44:15 2014 From: marielachertoff <@t> gmail.com (Mariela Chertoff) Date: Fri Jan 24 13:44:20 2014 Subject: [Histonet] Golgi staining Message-ID: Hi!!! I would like to ask you if you have a reliable protocol for Golgi staining of spines (without kit). Thanks in advance for your help, Best wishes, Mariela Chertoff, PhD Laboratorio de Biolog?a Molecular -QB75 Departamento de Qu?mica Biol?gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell?n II Piso 4 Ciudad de Buenos Aires - Argentina Tel: 54 11 4576-3300/09 - Int. 221 email: marielachertoff@gmail.com From jkiernan <@t> uwo.ca Fri Jan 24 14:02:32 2014 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Jan 24 14:02:37 2014 Subject: [Histonet] How to process tissues submitted in acrylamide In-Reply-To: <73508dbb147fb.52e2c6cd@uwo.ca> References: <7460825511ced.52e2c1d7@uwo.ca> <73d0a78a17cbd.52e2c213@uwo.ca> <73d0d5b914ae1.52e2c251@uwo.ca> <7460f77614af4.52e2c290@uwo.ca> <7460af7b17825.52e2c2ce@uwo.ca> <73d0c3f217dbc.52e2c386@uwo.ca> <7300eb8a15e13.52e2c3c4@uwo.ca> <7310dd2e105b4.52e2c3f5@uwo.ca> <7410896110a6f.52e2c651@uwo.ca> <73508a7316c0f.52e2c68f@uwo.ca> <73508dbb147fb.52e2c6cd@uwo.ca> Message-ID: <73c0ae3717aa7.52e28088@uwo.ca> If it's a cross-linked polyacrylamide gel (as used, for example, for electrophoresis), the block will suddenly shrink to a tiny stone-like object when the water content reaches a critical low level (15-20% water, 80-85% alcohol). I may have the % wrong; remembering an experiment in the early 1980s. You will need to do frozen sections. For technical details see Hausen,P & Dreyer,C (1981) Stain Technol. 56: 287-293. They didn't cryoprotect. Evidently its important not to let the sections dry out on the slides (or coverslips) before staining. John Kiernan Anatomy, UWO London, Canada = = = On 23/01/14, Catherine Simonson wrote: > Hello Helpful Histonetters, > > I have some samples that were fixed in PFA, placed in acrylamide gel and > then treated with iodine for microCT at another facility. The investigator > now wants to follow up and get H&E's on these samples. I know how to deal > with the iodine, but my question is this: what is the best procedure to > process these samples to slide? Do I run on a long processing schedule on > the processor, or do I do sucrose cryoprotection and get frozen. > Morphometry seems to be an important factor. > > Thanks in advance, > > Catherine Simonson, B.S., HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jkiernan <@t> uwo.ca Fri Jan 24 14:12:15 2014 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Jan 24 14:12:18 2014 Subject: [Histonet] Wright Stain on paraffin sections In-Reply-To: <73c0bea513421.52e2c8e3@uwo.ca> References: <5A590EB108038B4FA01F8CDB39C6541B01308DCD@EXCMBXPW7.chop.edu> <7380f04b14833.52e2c7ec@uwo.ca> <7350929f131e1.52e2c829@uwo.ca> <744085621729b.52e2c867@uwo.ca> <7440ff6014dfd.52e2c8a5@uwo.ca> <73c0bea513421.52e2c8e3@uwo.ca> Message-ID: <7410ff54178ed.52e282cf@uwo.ca> For formaldehyde-fixed material the pH has to be lower than for methanol-fixed blood or marrow smears (for which pH6.8 is best). For your sections try pH 5.0 first. Go down in steps of 0.5 if too blue; up in steps of 0.5 if too red. The colour scheme is not a varied as with a blood film, but it's prettier by far than H&E. John Kiernan Anatomy, UWO London, Canada = = = On 23/01/14, "Berger, Rebecca" wrote: > Hi all, > I am trying to look at eosinophils and neutrophils in FFPE muscle sections. Does anyone have experience with the Wright stain or a Wright staining kit on these types of sections? Or possibly a suggestion for a different protocol to visualize these cells? > Thanks! > Becky > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From kmilne <@t> bccancer.bc.ca Fri Jan 24 15:39:07 2014 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Fri Jan 24 15:39:13 2014 Subject: [Histonet] Mouse GranzymeB In-Reply-To: <8b0ea805-f52d-4e30-9d2b-4af65d6cca2f@SRVEXHT01.phsabc.ehcnet.ca> Message-ID: <3FEFF18FF4E1914A9AB7D8498591BE86031AA36E3A87@VEXCCR02.phsabc.ehcnet.ca> Hi Amos, We use cat # ab4059 from Abcam for our mouse (and human) granzyme B. It does have some nuclear background too but when we use it in conjunction with Biocare's Renaissance Background Reducing Diluent it cleans it up nicely. As you already have the Ab in, you could always try ordering some of the diluent (it's pretty cheap), see if that cleans it up. Good luck! Let me know if you have any other questions! :) Katy ------------------------------ Message: 21 Date: Fri, 24 Jan 2014 12:09:09 -0500 From: Amos Brooks Subject: [Histonet] Mouse GranzymeB To: histonet@lists.utsouthwestern.edu, "ihcrg Group, (E-mail)" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Ok Musketeers, I am trying to detect cytotoxic T-Cells in formalin fixed paraffin embedded mouse liver. GranzymeB should do the trick. I have a rabbit anti mouse (and human and rat) GranzymeB from abCam (ab53097). The spleen that I ran as a control with it works fine. Nice T-cells and no signifigant background staining. The liver on the others hand has some lymphocyte staining, presumably cytotoxic T-cells as expected, but it looks like every hepatocyte nuclei are also picking it up. Grrr, right? If this antibody had been raised in mouse, I wouldn't be surprised. Also, if the spleen looked similar, I wouldn't be surprised. Do any of you have any ideas ab pi ut what this might be? Alternatively, does anyone have any ideas about another antibody that would work here? CD4 or CD8 would be great here, but its FFPE mouse, so sadly that's out (more grumbling). For the completionists out there, I used EDTA pH9, peroxide block and detected the antibody (diluted 1:800) with Envision (rabbit) and DAB from Dako. Any suggestions would be vastly appreciated, Happy Friday, Amos ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 122, Issue 28 ***************************************** From azdudley <@t> hotmail.com Fri Jan 24 16:07:52 2014 From: azdudley <@t> hotmail.com (anita) Date: Fri Jan 24 16:07:56 2014 Subject: [Histonet] lysol Message-ID: Thanks so much to everyone for the info about lysol, I really appreciate it. Hope all have a great weekend. Anita From gayle.callis <@t> bresnan.net Fri Jan 24 16:49:00 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Jan 24 16:49:11 2014 Subject: [Histonet] Re: Activated charcoal for VIP 3000? Message-ID: <000901cf1956$7aa10ad0$6fe32070$@bresnan.net> Dear Mike, Our VIP K series had plastic containers to hold the charcoal and was refilled from bulk, and hopefully your VIP 3000 (you said 300?) has the same containers. I used charcoal for fish aquarium filters from a local pet store. It was cheaper than activated charcoal from other laboratory charcoal suppliers and although the charcoal pieces were a bit larger, the fume filtration still worked. One reason for trying this was charcoal from Sakura for the K series processors was being discontinued for older models, and a bit of ingenuity with lack of funding drove me to try it. Newer aquarium charcoal can be smaller as I refill my kitchen over the stove top filter at home since I can't find the replacement filters anymore. It may take some price comparison at PetSmart and Petco versus what you have found already. Buying in bulk would be ideal. Try Amazon and/or do a Google search. Gayle Callis HTL/HT/MT(ASCP) From tgenade <@t> gmail.com Sun Jan 26 09:46:30 2014 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Sun Jan 26 09:46:35 2014 Subject: [Histonet] Re: Golgi staining (Histonet Digest, Vol 122, Issue 29) Message-ID: Hello Mariela, > From: Mariela Chertoff > Subject: [Histonet] Golgi staining > I would like to ask you if you have a reliable protocol for Golgi staining > of spines (without kit). You can try the one at http://www.ncbi.nlm.nih.gov/pubmed/21228908 . I tried it and it did stain my fish but the 37 oC trick wasn't useful for me 25 oC fish... My staining was never as intense as shown in the article. The authors were also very helpful in trouble shooting. But, to be honest, it might be cheaper and less frustrating to talk with Ronald F. Mervis (http://www.neurostructural.org/) and find out how much his help will cost. He has Golgi staining down to a fine art. Tyrone Genade From cbrya <@t> lexclin.com Mon Jan 27 10:44:09 2014 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Mon Jan 27 10:44:16 2014 Subject: [Histonet] artifact caused by freezing of tissues during transport Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF4BF843B462@EXCHANGESB> Has anyone experienced artifact caused by freezing of tissues during transport with these frigid days? If so, how long does it take specimens submitted in 10% NBF to freeze while being transported to a laboratory? Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From mucram11 <@t> comcast.net Mon Jan 27 13:04:11 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon Jan 27 13:04:31 2014 Subject: [Histonet] Arkansas Society of Histotechnology Spring Meeting in Hot Springs AR Message-ID: <2109461375.120975.1390849451184.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Please join us for the meeting and if you need a registration form of further information please contact me by e-mail today!!? Pam Marcum ? ? WELCOME TO THE 40 TH ANNUAL MEETING OF THE ARKANSAS SOCIETY FOR HISTOTECHNOLOGY ? February 28 th ? FRIDAY MORNING WORKSHOPS ? 8:00AM to 11:30AM ? ALL MORNING AND AFTERNOON BREAKS AT 9:30AM AND 2:30PM IN THE DESOTO BALLROOM LUNCH FRIDAY AND SATURDAY WILL BE AT 11:30AM IN THE VENDOR AREA OF THE DESOTO BALLROOM A SHORT BUSINESS MEETING WILL BE HELD ON SATURDAY FROM 12:30PM TO 1:00PM ? Workshop 1 - Mountain Tower Room ?????????? 8:00AM TO 11:30PM ? IHC from the Beginning ? Sponsored by Lab Storage Systems ???????? Bonnie Whitaker, Anatomic Pathology Operations Director Ohio State Medical Center Columbus, Ohio ? In today?s clinical laboratories, it is a common practice to hire inexperienced staff for the IHC laboratory. ? Since instrumentation is used extensively, it is less imperative for staff to have experience with IHC techniques, or to understand the principles, than it was at one time. ? It is extremely critical, for those who wish to excel in the laboratory, and to be able to troubleshoot IHC techniques, to understand what actually happens to the slides and why. ? In this workshop, the basic concepts of IHC will be presented in a way that will help the novice IHC tech understand exactly what has to happen, and why, in order to get a top quality IHC slide. ? Workshop 2 ? Pageant Room ??????????????????????? 8:00AM TO 9:30PM ? 90Minutes Speaker To Be Announced. Workshop 3 ? Pageant Room ??????????????????????? 10:00AM to 11:30AM ? 90 minutes Mobile Histology Dr Shree Sharma UAMS Department of Anatomic Pathology ? Mobile technology is bringing change in the way we live, see and perceive the world. In this talk we will explore how we can integrate fast evolving technology in our working place. How we can have apps which can help in monitoring and maintaining our labs and improving our skills. ? February 28th AFTERNOON WORKSHOPS 1:00PM to 4:30PM ? WORKSHOP 3 - Mountain Tower Room ??????? 1:00PM TO 4:30PM ? I HAVE NO IDEA WHAT I AM LOOKING AT!! Shane Jones BS, HT (ASCP) School of Histotechnology Program Director Baptist Health Schools Little Rock AR ? Have you ever looked at an H & E or a special stain in the microscope and you wish you could identify the tissue you were looking at, or maybe remember from school how to identify it? Good News! This workshop will take specific histologic keys of the 4 major tissue types and 18 different organs in order to give you the ability to identify them microscopically. ? WORKSHOP 4 ? PAGEANT ROOM ??????????????? 1:00PM TO 4:30PM ? What?s New in HER2 Treatment, Testing, and Regulation? Lynn Charpentier, Marketing Product Manager DAKO an Agilent Company Carpinteria CA ? An overview of companion diagnostics and targeted therapies ? Information on the two newest HER2 targeted treatments A review of the new CAP/ASCO HER2 Guidelines released in September 2013 with a focus on what has changed for the laboratory and pathologist An introduction to the new FISH methodology utilizing Ethylene Carbonate and its application in HER2 testing ? FRIDAY NIGHT MEET AND GREET AT 6:00PM IN THE DESOTO BALLROOM WITH VENDORS. ?? ROARING TWENTIES THEME SO BE READY TO do the CHARLESTON AND ENJOY THE COMPANY. ? CASH BAR WILL BE AVAILABLE IN THE BALLROOM ? ? MARCH 1 - SATURDAY MORNING 8:00AM TO 11:30AM ? WORKSHOP 5 - MOUNTAIN TOWER ROOM ?????????????????? 8:00AM TO 11:30PM ? Hazard Communication Standard/GHS Awareness and Formaldehyde Training Richard Best, Technical Director and Corporate Director of OSHA Compliance Stericycle, Inc. Benton AR ? Part One: ? 90 MINUTES These are new regulations for waste handling today in Part 1 and Part 2 This is an important class to cover the new Globally Harmonized System (GHS) adopted by OSHA in 2012 and the compliance changes to the Hazard Communication Standard. Employees must have been trained regarding the new label elements and Safety Data Sheet format by December 1, 2013. ? Part Two: 90 MINUTES Formaldehyde Awareness Training ? The requirements of OSHA's Formaldehyde Standard 1910.1048 ?? Regulated Waste Streams Understanding how to properly segregate specialty waste streams and handle the most common regulated wastes. Requirements for preparing for transport are covered. Medical Waste and Resource Conservation and Recovery Act Hazardous Waste are both covered in this presentation. ? WORKSHOP 6 ? PAGEANT ROOM ??????????????????????????????????? 8:00AM TO 11:30PM ? Basic Microtomy Mari Ann Mailhiot, Technical Specialist Leica Biosystems Richmond IL ? Microtomy is the basis of our daily lives in Histology and the need to understand how to best use the microtomes as they have evolved over the past 30+ years. ? The motorized microtomes of today add life to our ability to section better and in combination with the sharper knives have improved our work. ? Improvements in basic ergonomics combined with other advances allow fewer repetitive work injuries. ? MARCH 1 - SATURDAY AFTERNOON 1:00PM TO 4:30PM ? WORKSHOP 7 ? MOUNTAIN TOWER ROOM ????????????????? 1:00PM TO 4:30PM ? Basic IHC and Troubleshooting Workshop Matthew Pardilla, Technical Consultant Cell Marque Corporation Rocklin, CA ? This workshop is designed to improve basic IHC knowledge and uncover what goes on ?behind the scenes? inside your IHC automated platform. It will touch on different types of detection, explaining the advantages and disadvantages of each along with how to determine the best one for your assay. We will then address the most common causes of suboptimal staining and how to troubleshoot different scenarios. Additionally, the session will open up to participants? troubleshooting questions and we will solve them as a group. ? WORKSHOP 8 ? PAGEANT ROOM ??????????????????? ??????????????? 1:00PM TO 4:30PM ? Unraveling the Mysteries of Histotechnology ?????????????????????????????? ? Debbie Sienna Statlab Medical Products McKinney TX ? Have you ever wondered?about some of the products that we use in the laboratory, for instance the slides that we use every day? ? Why some slides are green and some white and what does that mean in everyday laboratory life? ? Have you heard of hydrophilic slides and hydrophobic slides and wondered what the differences are? ? There are some products that we in histology use on a daily basis and yet we don?t always know why they are the way that they are or what that could mean to us and so we are not always open to making changes in the products that we use, even if we may be able to save some serious cash. ? However, in these times of reimbursement cuts, keeping the budget under control may be of the utmost importance in the survival of the laboratory. ? In this workshop we will uncover some of the facts and fiction behind the most essential products in the lab, slides, paraffin, microtome blades and even alcohol, xylene and xylene substitutes. ? Participants should come away from the workshop with a better understanding of how the products have been developed as well as knowledge of what makes a difference and what doesn?t. ?? ? WORKSHOP 9 TRI LAKES ROOM ? MARCH 1 ST SATURDAY ? CLASS BEGINS AT 8:00AM AND ENDS AT 4:30PM SPECIAL CLASS ALL FOR HISTOLOGY REGISTRY COURSE ? I HAVE TO KNOW ALL OF THAT FOR THE EXAM??? ?????? ? Shane Jones BS, HT (ASCP) Baptist Health Schools Little Rock School of Histotechnology Program Director Little Rock AR ? Studying for the ASCP Board of Registry Exam is a huge undertaking. That difficulty is increased even more when you are doing it on your own. The amount of time and discipline it takes can be overwhelming. Saying that 6 hours is nowhere near adequate to cover what you need to study for the Board of Registry Exam. In this workshop, we will do our best to cover the same subject matter that I use for my students each year. We will cover the information with the 5 subcategories of the Board of Registry Exam in mind. These subject matter within these subcategories will include: instrumentation and its maintenance used in the histology lab, safety issues and guidelines that are set up by various government agencies, laboratory math and chemistry basic level, the four major tissue types and eighteen different organs will be microscopically identified, chemicals that are used in tissue processing (fixatives, dehydrating agents, clearing agents, and infiltrating medias) will be identified and their advantages and disadvantages will be discussed, embedding and microtomy methodologies and troubleshooting will be addressed, the H & E stain, ? the 4 methods of decalcification and methods for testing endpoint, and finally we will address special stains (their mode of action, components and their functions, what they are recommended for, and results of the stain. From tony.henwood <@t> health.nsw.gov.au Mon Jan 27 23:40:53 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Jan 27 23:41:23 2014 Subject: [Histonet] RE: artifact caused by freezing of tissues during transport In-Reply-To: <50DA0C6B72976B4AB3A0FCA04CC73DBF4BF843B462@EXCHANGESB> References: <50DA0C6B72976B4AB3A0FCA04CC73DBF4BF843B462@EXCHANGESB> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00AB65B@xmdb04.nch.kids> Hi Carol, Freezing of tissue whilst in fixative is bad news. The artefacts can be horrendous. eg: Rosen, Y., & Ahuja, S. C. (1977). Ice crystal distortion of formalin-fixed tissues following freezing. The American Journal of Surgical Pathology, 1(2), 179-182. Westenend, P. J. (2004). Incidental freezing artefacts in sentinel lymph node biopsies masquerading as lymphangiography artefacts. Journal of clinical pathology, 57(6), 671. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Tuesday, 28 January 2014 3:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] artifact caused by freezing of tissues during transport Has anyone experienced artifact caused by freezing of tissues during transport with these frigid days? If so, how long does it take specimens submitted in 10% NBF to freeze while being transported to a laboratory? Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From pruegg <@t> ihctech.net Tue Jan 28 13:10:31 2014 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Tue Jan 28 13:10:38 2014 Subject: [Histonet] Mouse GranzymeB In-Reply-To: References: Message-ID: <002e01cf1c5c$9df03d90$d9d0b8b0$@ihctech.net> Amos u might have to get an anti rabbit link that has been mouse absorbed. Dako's anti rab envision is made in a goat but it is not mouse absorbed. Southern Biotec or Jackson Labs are really good places to get absorbed links. Still no reliable cd4 and cd8 for ffpe mouse tissue as far as I know, I have tried many that claim they work but have not consistently worked in my hands, we still do that with frozen mouse tissue not aldehyde fixed. Are u doing HIER with the EDTA ph9? In my experience especially with the higher ph u can over retrieve and cause undesirable nuclear staining. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com ? This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, January 24, 2014 10:09 AM To: histonet@lists.utsouthwestern.edu; ihcrg Group, (E-mail) Subject: [Histonet] Mouse GranzymeB Ok Musketeers, I am trying to detect cytotoxic T-Cells in formalin fixed paraffin embedded mouse liver. GranzymeB should do the trick. I have a rabbit anti mouse (and human and rat) GranzymeB from abCam (ab53097). The spleen that I ran as a control with it works fine. Nice T-cells and no signifigant background staining. The liver on the others hand has some lymphocyte staining, presumably cytotoxic T-cells as expected, but it looks like every hepatocyte nuclei are also picking it up. Grrr, right? If this antibody had been raised in mouse, I wouldn't be surprised. Also, if the spleen looked similar, I wouldn't be surprised. Do any of you have any ideas ab pi ut what this might be? Alternatively, does anyone have any ideas about another antibody that would work here? CD4 or CD8 would be great here, but its FFPE mouse, so sadly that's out (more grumbling). For the completionists out there, I used EDTA pH9, peroxide block and detected the antibody (diluted 1:800) with Envision (rabbit) and DAB from Dako. Any suggestions would be vastly appreciated, Happy Friday, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Jan 28 13:26:25 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Jan 28 13:26:32 2014 Subject: [IHCRG] RE: [Histonet] Mouse GranzymeB In-Reply-To: <002e01cf1c5c$9df03d90$d9d0b8b0$@ihctech.net> References: <002e01cf1c5c$9df03d90$d9d0b8b0$@ihctech.net> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05249@SBS2K8.premierlab.local> Patsy and Amos I can appreciate your comment but we have been using Dako's Envision+ Rabbit on mouse tissue for years now and have never seen any cross reactivity to mouse tissue, it's pretty much our go to detection system for rabbit antibodies (both monoclonal and polyclonal) on mouse tissue. We have however seen cross reactivity to porcine with Dako's envision +mouse and other companies anti-mouse polymer reagents. My suggestion if you have not already done this is to try to use some other retrieval methods such as pH6 or enzyme (proteinase K or pepsin or even no retrieval). Tissue fixation is important along with time an temp of retrieval - under fixation or over retrieval or a combination of both can cause problems. If we find we are getting some background we will run some more intense blocking - such as the following: 1. commercially available superblock - these can be competitive and may potentially cause decreased sensitivity so you will need to watch out on these 2. Normal serum - we normally use a commercial serum free protein block but on occasions we will use up to a 20% normal serum block for 30 to 60 minutes, if you want to continue to use the envision+ rabbit your choice would be 20% normal goat serum 3. Saturated Casein - we'll make this up in house All are worth a try, good luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of pruegg@ihctech.net Sent: Tuesday, January 28, 2014 12:11 PM To: 'Amos Brooks'; histonet@lists.utsouthwestern.edu; 'ihcrg Group, (E-mail)' Subject: [IHCRG] RE: [Histonet] Mouse GranzymeB Amos u might have to get an anti rabbit link that has been mouse absorbed. Dako's anti rab envision is made in a goat but it is not mouse absorbed. Southern Biotec or Jackson Labs are really good places to get absorbed links. Still no reliable cd4 and cd8 for ffpe mouse tissue as far as I know, I have tried many that claim they work but have not consistently worked in my hands, we still do that with frozen mouse tissue not aldehyde fixed. Are u doing HIER with the EDTA ph9? In my experience especially with the higher ph u can over retrieve and cause undesirable nuclear staining. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com ? This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, January 24, 2014 10:09 AM To: histonet@lists.utsouthwestern.edu; ihcrg Group, (E-mail) Subject: [Histonet] Mouse GranzymeB Ok Musketeers, I am trying to detect cytotoxic T-Cells in formalin fixed paraffin embedded mouse liver. GranzymeB should do the trick. I have a rabbit anti mouse (and human and rat) GranzymeB from abCam (ab53097). The spleen that I ran as a control with it works fine. Nice T-cells and no signifigant background staining. The liver on the others hand has some lymphocyte staining, presumably cytotoxic T-cells as expected, but it looks like every hepatocyte nuclei are also picking it up. Grrr, right? If this antibody had been raised in mouse, I wouldn't be surprised. Also, if the spleen looked similar, I wouldn't be surprised. Do any of you have any ideas ab pi ut what this might be? Alternatively, does anyone have any ideas about another antibody that would work here? CD4 or CD8 would be great here, but its FFPE mouse, so sadly that's out (more grumbling). For the completionists out there, I used EDTA pH9, peroxide block and detected the antibody (diluted 1:800) with Envision (rabbit) and DAB from Dako. Any suggestions would be vastly appreciated, Happy Friday, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups "ihcrg" group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscribe@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. From pruegg <@t> ihctech.net Tue Jan 28 14:14:01 2014 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Tue Jan 28 14:14:07 2014 Subject: [IHCRG] RE: [Histonet] Mouse GranzymeB In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E05249@SBS2K8.premierlab.local> References: <002e01cf1c5c$9df03d90$d9d0b8b0$@ihctech.net> <14E2C6176416974295479C64A11CB9AE019C79E05249@SBS2K8.premierlab.local> Message-ID: <003b01cf1c65$7cc391e0$764ab5a0$@ihctech.net> I agree with what Liz has said. Usually when I encounter undesirable bg staining especially if it is nuclear it most often points to over retrieval of tissues not adequately fixed. Besides using less harsh retrieval such as low ph HIER, EIER, or no IER Loui Harkin is a big believer of prefixing questionably fixed tissue sections after depar in formalin before HIER and that has worked for me in the past as well. I almost routinely use serum free protein block (casein) and in difficult cases replace that with 20% serum from the species the secondary is made in. Dako anti rab Envision+ LP and Leica's anti rab Power Vision LP are my go too reagents for using rab antibodies on ffpe mouse tissue. Leica Bonds detection system can be used for this as well if u skip the post primary which is rabbit anti mouse and just go direction to the goat anti rab labeled polymer detection. Good luck, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com ? -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Elizabeth Chlipala Sent: Tuesday, January 28, 2014 12:26 PM To: pruegg@ihctech.net; 'Amos Brooks'; histonet@lists.utsouthwestern.edu; 'ihcrg Group, (E-mail)' Subject: RE: [IHCRG] RE: [Histonet] Mouse GranzymeB Patsy and Amos I can appreciate your comment but we have been using Dako's Envision+ Rabbit on mouse tissue for years now and have never seen any cross reactivity to mouse tissue, it's pretty much our go to detection system for rabbit antibodies (both monoclonal and polyclonal) on mouse tissue. We have however seen cross reactivity to porcine with Dako's envision +mouse and other companies anti-mouse polymer reagents. My suggestion if you have not already done this is to try to use some other retrieval methods such as pH6 or enzyme (proteinase K or pepsin or even no retrieval). Tissue fixation is important along with time an temp of retrieval - under fixation or over retrieval or a combination of both can cause problems. If we find we are getting some background we will run some more intense blocking - such as the following: 1. commercially available superblock - these can be competitive and may potentially cause decreased sensitivity so you will need to watch out on these 2. Normal serum - we normally use a commercial serum free protein block but on occasions we will use up to a 20% normal serum block for 30 to 60 minutes, if you want to continue to use the envision+ rabbit your choice would be 20% normal goat serum 3. Saturated Casein - we'll make this up in house All are worth a try, good luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of pruegg@ihctech.net Sent: Tuesday, January 28, 2014 12:11 PM To: 'Amos Brooks'; histonet@lists.utsouthwestern.edu; 'ihcrg Group, (E-mail)' Subject: [IHCRG] RE: [Histonet] Mouse GranzymeB Amos u might have to get an anti rabbit link that has been mouse absorbed. Dako's anti rab envision is made in a goat but it is not mouse absorbed. Southern Biotec or Jackson Labs are really good places to get absorbed links. Still no reliable cd4 and cd8 for ffpe mouse tissue as far as I know, I have tried many that claim they work but have not consistently worked in my hands, we still do that with frozen mouse tissue not aldehyde fixed. Are u doing HIER with the EDTA ph9? In my experience especially with the higher ph u can over retrieve and cause undesirable nuclear staining. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com rueggihcconsultingpr@outlook.com ? This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, January 24, 2014 10:09 AM To: histonet@lists.utsouthwestern.edu; ihcrg Group, (E-mail) Subject: [Histonet] Mouse GranzymeB Ok Musketeers, I am trying to detect cytotoxic T-Cells in formalin fixed paraffin embedded mouse liver. GranzymeB should do the trick. I have a rabbit anti mouse (and human and rat) GranzymeB from abCam (ab53097). The spleen that I ran as a control with it works fine. Nice T-cells and no signifigant background staining. The liver on the others hand has some lymphocyte staining, presumably cytotoxic T-cells as expected, but it looks like every hepatocyte nuclei are also picking it up. Grrr, right? If this antibody had been raised in mouse, I wouldn't be surprised. Also, if the spleen looked similar, I wouldn't be surprised. Do any of you have any ideas ab pi ut what this might be? Alternatively, does anyone have any ideas about another antibody that would work here? CD4 or CD8 would be great here, but its FFPE mouse, so sadly that's out (more grumbling). For the completionists out there, I used EDTA pH9, peroxide block and detected the antibody (diluted 1:800) with Envision (rabbit) and DAB from Dako. Any suggestions would be vastly appreciated, Happy Friday, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups "ihcrg" group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscribe@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups "ihcrg" group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscribe@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. From TNMayer <@t> mdanderson.org Wed Jan 29 07:58:30 2014 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Jan 29 08:01:53 2014 Subject: [Histonet] RE: artifact caused by freezing of tissues during transport Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8802C9317A@D1PWPEXMBX05.mdanderson.edu> Check with your local LabCorp, or Quest Diagnostics. They get tissues frozen in 10% NBF periodically due to inexperienced couriers putting them on dry ice. They may have worked something out. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 1 Date: Mon, 27 Jan 2014 11:44:09 -0500 From: Carol Bryant Subject: [Histonet] artifact caused by freezing of tissues during transport To: "histonet@lists.utsouthwestern.edu" Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF4BF843B462@EXCHANGESB> Content-Type: text/plain; charset="us-ascii" Has anyone experienced artifact caused by freezing of tissues during transport with these frigid days? If so, how long does it take specimens submitted in 10% NBF to freeze while being transported to a laboratory? Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com From HornHV <@t> archildrens.org Wed Jan 29 11:34:12 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jan 29 11:34:21 2014 Subject: [Histonet] oil red o on cytology Message-ID: <25A4DE08332B19499904459F00AAACB719DE6DEC24@EVS1.archildrens.org> What kind of control do you use when looking for fat in a BAL specimen? This stain is done on a cytospin slide. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Vickroy.Jim <@t> mhsil.com Wed Jan 29 14:59:33 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Jan 29 14:59:39 2014 Subject: [Histonet] CoPath Plus IHC orders Message-ID: We are in the process of exploring the interface that Cerner has for sending information from CoPath Plus (ABT) to our Ventana Special stainer and Benchmark Ultras. I am told that large users have found the interface to be a time-saving tool. I would be interested in hearing from someone that has the interface between CoPath Plus and the Ventana Instruments to find out how well it actually works. We have had CoPath plus for several years and have problems when our pathologists order special stains, recuts, and IHC stains. We finally got it to work (without losing ordered cases) by running a log on the hour every hour. I believe the log is called Stain/Process Log Verify/Print. The problems we encounter with this log is that: 1. It only prints on the hour. (We tried setting it up for a shorter time but experienced duplicates and other problems.) So if our pathologist orders a stain it may be 59 minutes before we get the order in the lab. 2. Sometimes if the pathologist orders a stain close to the printing time a case would end up not showing up on the log. 3. If we tried to run additional logs it sometimes affected if a case was on the printed log. As you can see if the order interface would transfer the information to the Ventana computers we would first cut down on clerical time and mistakes. We would also be able to get the orders in a more efficient time instead of waiting for an hourly log to print. We have been hesitant to play with the current system since we have had occasions when the cases get left of logs. We have also experimented with other logs built in Copath but have not come up with a viable fix. Frankly CoPath has been less than helpful. My worry is that if we purchase the interfaces then we will have similar problems and perhaps additional problems. Would anyone be willing to share their experiences with these systems and any suggestions they would have to approach the interface or the problems with the logs? Thank you Jim Vickroy James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From gayle.callis <@t> bresnan.net Wed Jan 29 18:40:04 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Jan 29 18:40:25 2014 Subject: [Histonet] Need help with a publication reference Message-ID: <000101cf1d53$d3432dc0$79c98940$@bresnan.net> Dear Histonetters, If anyone has an original copy of Woods and Ellis, Laboratory Histopathology: A Complete Reference, 1994 Churchill Livingstone, I would like to know the chapter and page numbers for Anthony S-Y Leong, " Fixation and Fixatives" chapter. Title of book, publisher, date and ISBN # is fine. Unfortunately, the book is out of print. What I found on the internet for Roy Ellis Histology Page doesn't not have the page numbers for the Leong Fixation "excerpt". And the email address for Roy Ellis is no longer valid or up to date. Unfortunately, publishers need chapters and pages in references. If anyone has the book and has the time, a pdf copy of the table of contents would very helpful. Thank you for your help Gayle M. Callis HTL/HT/MT(ASCP) From powderhound34 <@t> hotmail.com Wed Jan 29 19:17:16 2014 From: powderhound34 <@t> hotmail.com (joe joe) Date: Wed Jan 29 19:17:20 2014 Subject: [Histonet] Dako's Omnis In-Reply-To: References: Message-ID: Can anyone share their experiences with me about Dako's new IHC / FISH instrument? Thanks in advance! From tmcampbell <@t> fmh.org Thu Jan 30 06:09:54 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Thu Jan 30 06:09:42 2014 Subject: [Histonet] Dako's Omnis In-Reply-To: References: Message-ID: <3566D9E34287BE4B95372179009446A019AD8A3B@EXCHANGE.fmhnt.fmh.org> Are you talking about there IHC stainer, the Link? And there FISH kits or is there a new instrument that stains FISH? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joe joe Sent: Wednesday, January 29, 2014 8:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako's Omnis Can anyone share their experiences with me about Dako's new IHC / FISH instrument? Thanks in advance! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Jan 30 08:40:47 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jan 30 08:41:19 2014 Subject: [Histonet] RELIA Histology Careers Weekly Update 1-30-2014 Are you Ready for Super Bowl Sunday? Message-ID: <007c01cf1dc9$4ff17550$efd45ff0$@earthlink.net> Hi Histonetters!! I hope your week is going great. Are you Gearing up for the Super Bowl? Or the Commercials?? How About the 10th Annual Puppy Bowl? Or the Inaugural Kitten Bowl? It's almost like a National Holiday! And it's this Sunday!! Looks like these days there is something for everybody on Super Bowl Sunday!! Before I gear up for the big weekend and whip up a batch of my killer nachos, I thought I would drop you a line with my latest most current positions. Of course all of these positions are permanent and full time. All of my clients offer excellent compensation, benefits and in most cases relocation is negotiable. These clients are currently interviewing and ready to hire! Here is a list of my current openings: HISTOLOGY MANAGEMENT: Pathology/Histology Supervisor - Cape Cod, MA Histology Supervisor - Atlanta, GA Histology Supervisor - Zanesville, OH HISTOLOGY TECHNICIAN/TECHNOLOGIST 3rd Shift Grossing Histotechnologist - Boston, MA 2nd shift Histology Tech - Boston, MA Dermpath Histotech - Atlanta, GA Grossing Tech - Austin, TX Cytogenetics Tech - Louisville, KY FISH Tech - Louisville, KY Cytotech - Lafayette, LA More positions are coming in all of the time so if you are looking let me know where and what you are looking for and I will keep you posted. ****REMEMBER. It never hurts to look!***** You can reach me toll free at 866-607-3542 until 6 EST and anytime on my cell phone at 407-353-5070, or shoot me an email at relia1@earthlink.net to schedule a time to talk. I really appreciate you taking the time to read this e-mail. It means a lot to me when you take the time to refer your friends and coworkers. And to show my appreciation, I offer you a 500.00 referral fee for anyone you refer to me that I place. Have a Great time on Super Bowl Sunday Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From avistarop <@t> ffyb.uba.ar Thu Jan 30 09:04:07 2014 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Thu Jan 30 09:06:40 2014 Subject: [Histonet] EBV EBNA3A antibody (ab16126, Abcam) Message-ID: <0c6b54e490703dd2712cf11dd37d094d.squirrel@huemul.ffyb.uba.ar> Hello to everyone!!! Has anyone used this antibody ? Sheep polyclonal to EBV EBNA3A antibody (ab16126, Abcam) This antibody is used on FFPE tissue. Could I please get a copy of your protocols? Thank you so much Aldana Vistarop From Vickroy.Jim <@t> mhsil.com Thu Jan 30 09:32:19 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Jan 30 09:32:25 2014 Subject: [Histonet] Cryostat Disinfection Message-ID: In the CAP checklist it states that The cryostat must be4 defrosted and decontaminated by wiping all exposed surfaces with a tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. The newest cryostat by Thermofisher uses cold disinfection with a chemical called Sanosil. Am I right thinking that on that cryostat we do not have to disinfect at room temperature? Your thoughts? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From relia1 <@t> earthlink.net Thu Jan 30 09:33:07 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jan 30 09:33:27 2014 Subject: [Histonet] RELIA Histology Careers Weekly Update 1-30-2014 Are you Ready for Super Bowl Sunday? Message-ID: <00c301cf1dd0$93552e70$b9ff8b50$@earthlink.net> Hi Histonetters!! I hope your week is going great. Are you Gearing up for the Super Bowl? Or the Commercials?? How About the 10th Annual Puppy Bowl? Or the Inaugural Kitten Bowl? It's almost like a National Holiday! And it's this Sunday!! Looks like these days there is something for everybody on Super Bowl Sunday!! Before I gear up for the big weekend and whip up a batch of my killer nachos, I thought I would drop you a line with my latest most current positions. Of course all of these positions are permanent and full time. All of my clients offer excellent compensation, benefits and in most cases relocation is negotiable. These clients are currently interviewing and ready to hire! Here is a list of my current openings: HISTOLOGY MANAGEMENT: Pathology/Histology Supervisor - Cape Cod, MA Histology Supervisor - Atlanta, GA Histology Supervisor - Zanesville, OH HISTOLOGY TECHNICIAN/TECHNOLOGIST 3rd Shift Grossing Histotechnologist - Boston, MA 2nd shift Histology Tech - Boston, MA Dermpath Histotech - Atlanta, GA Grossing Tech - Austin, TX Cytogenetics Tech - Louisville, KY FISH Tech - Louisville, KY Cytotech - Lafayette, LA More positions are coming in all of the time so if you are looking let me know where and what you are looking for and I will keep you posted. ****REMEMBER. It never hurts to look!***** You can reach me toll free at 866-607-3542 until 6 EST and anytime on my cell phone at 407-353-5070, or shoot me an email at relia1@earthlink.net to schedule a time to talk. I really appreciate you taking the time to read this e-mail. It means a lot to me when you take the time to refer your friends and coworkers. And to show my appreciation, I offer you a 500.00 referral fee for anyone you refer to me that I place. Have a Great time on Super Bowl Sunday Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From ARodriguez <@t> emc.org Thu Jan 30 13:21:14 2014 From: ARodriguez <@t> emc.org (Rodriguez, Arnold) Date: Thu Jan 30 13:21:21 2014 Subject: [Histonet] Digital dictation system Message-ID: <2EF32B926156C643973C40BCF72682AF04193C84@NT269.info.sys> Which are the best digital dictation systems currently available for grossing? Our LIS is CoPathPlus but they don't have a dictation module. Arnold Rodriguez, HT, ASCP Histology Supervisor Eisenhower Medical Center 39000 Bob Hope Drive Rancho Mirage, Ca 92270 Phone (760) 773-2013 Fax (760) 773-1587 email: Arodriguez@emc.org From ccrowder <@t> vetmed.lsu.edu Thu Jan 30 13:59:32 2014 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Jan 30 14:00:11 2014 Subject: [Histonet] marking tiny specimens Message-ID: I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Jan 30 14:10:09 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Jan 30 14:10:45 2014 Subject: [Histonet] marking tiny specimens In-Reply-To: References: Message-ID: <2d61c62e716545ff9d623ea4194c7b5d@BL2PR04MB196.namprd04.prod.outlook.com> Are you mixing Eosin in the last two alcohols of your processor? That should work. If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing. Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink. I would do this under a dissecting microscope. Maybe you could do that with the eggs? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cheryl Crowder Sent: Thursday, January 30, 2014 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking tiny specimens I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Thu Jan 30 14:12:09 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Jan 30 14:12:13 2014 Subject: [Histonet] Ventana LEAN team In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC011245D90D@JERRY.Gia.com> Has anyone had experience with Ventana's Lean team? I am correctly designing a new lab and they are coming out next tues to look at my workflow. Just wondering what to expect and how much they may change my current layout of my future lab :) Thanks! From liz <@t> premierlab.com Thu Jan 30 14:14:39 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jan 30 14:14:44 2014 Subject: [Histonet] marking tiny specimens In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0527C@SBS2K8.premierlab.local> Cheryl So the eggs don't absorb the eosin at all or does the dye come out during processing? We use eosin all of the time for samples like that, but I leave them in eosin for about 10 minutes. Here is what we do, the fixed samples are normally submitted in Eppendorf tubes, we remove the formalin and replace with eosin (I use Anatechs), let sit for about 10 minutes and then very gently we use a plastic disposable pipette (the ones with bulbs) we place the samples in a tea bag and process on a very short cycle on the tissue processor (5 -10 minutes per station) we do this during the day so it's a continuous process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Thursday, January 30, 2014 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking tiny specimens I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Thu Jan 30 14:39:01 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Jan 30 14:39:07 2014 Subject: [Histonet] marking tiny specimens In-Reply-To: References: Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276B17EBAA@CVM76.vetmed.wsu.edu> Hi Cheryl, If you can, use more eggs. Then process them into a pellet with histogel. Upon sectioning, many different cross-sections will be visible in the same section. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Thursday, January 30, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking tiny specimens I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Thu Jan 30 14:29:43 2014 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Thu Jan 30 14:44:59 2014 Subject: [Histonet] RE: Ventana LEAN team In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011245D90D@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC011245D90D@JERRY.Gia.com> Message-ID: Hi Amber, As part of our Vantage implementation process, a workflow consultant initially came out to look at our processes. He was very nice and had a lot of great suggestions. He was never pushy and I had control of what I wanted to change or not change. He basically was there to see how the implementation would go according to what we wanted and how things were set up. Having his overall assessment of our workflow and how we could improve steps was very helpful with our final implementation of the Vantage system. Even if you are not getting the Vantage Specimen Tracking System, the workflow assessment will help you in your overall lab design. Use his/her suggestions as you want... It's your lab... We are all unique. :) Good luck, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Thursday, January 30, 2014 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LEAN team Has anyone had experience with Ventana's Lean team? I am correctly designing a new lab and they are coming out next tues to look at my workflow. Just wondering what to expect and how much they may change my current layout of my future lab :) Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Thu Jan 30 15:03:29 2014 From: suetp918 <@t> comcast.net (Sue) Date: Thu Jan 30 15:05:42 2014 Subject: [Histonet] Digital dictation system In-Reply-To: <2EF32B926156C643973C40BCF72682AF04193C84@NT269.info.sys> References: <2EF32B926156C643973C40BCF72682AF04193C84@NT269.info.sys> Message-ID: <04F4E176-1649-4FDF-A372-4868ABFDB856@comcast.net> Dragon use it with copath no issues Sent from my iPhone > On Jan 30, 2014, at 2:21 PM, "Rodriguez, Arnold" wrote: > > Which are the best digital dictation systems currently available for grossing? Our LIS is CoPathPlus but they don't have a dictation module. > > Arnold Rodriguez, HT, ASCP > Histology Supervisor > Eisenhower Medical Center > 39000 Bob Hope Drive > Rancho Mirage, Ca 92270 > Phone (760) 773-2013 > Fax (760) 773-1587 > email: Arodriguez@emc.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Wanda.Smith <@t> HCAhealthcare.com Thu Jan 30 15:07:58 2014 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Jan 30 15:08:40 2014 Subject: [Histonet] marking tiny specimens In-Reply-To: References: Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27EB9149AB@NADCWPMSGCMS03.hca.corpad.net> We use a dropper of hematoxylin at the grossing table and it stays on the tissue even after processing. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Thursday, January 30, 2014 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking tiny specimens I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Fri Jan 31 00:21:42 2014 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Jan 31 00:21:59 2014 Subject: [Histonet] marking tiny specimens In-Reply-To: <7400e1fc16eb2.52eb408d@uwo.ca> References: <2d61c62e716545ff9d623ea4194c7b5d@BL2PR04MB196.namprd04.prod.outlook.com> <7390834c14a59.52eb3f1d@uwo.ca> <73a0c4f116ca7.52eb3f5a@uwo.ca> <7400ee8d12ef6.52eb3f98@uwo.ca> <731083d91792c.52eb404e@uwo.ca> <7400e1fc16eb2.52eb408d@uwo.ca> Message-ID: <73a0afa0133b2.52eafaa6@uwo.ca> Dedication to duty! Ouch! Did you ever run out of eyelashes? Why not a short eyebrow or forearm hair? JK = = = On 30/01/14, Barbara Tibbs wrote: > Are you mixing Eosin in the last two alcohols of your processor? That should work. If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing. > > Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink. I would do this under a dissecting microscope. Maybe you could do that with the eggs? > > Barbara S. Tibbs > Histology Supervisor > Accurate Diagnostic Labs > South Plainfield, NJ > barbara.tibbs@accuratediagnosticlabs.com > 732-839-3374 > Cell: 610-809-6508 > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cheryl Crowder > Sent: Thursday, January 30, 2014 5:59 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] marking tiny specimens > > I am processing some extremely small specimens - pin tip size. These are > eggs with a protein covering. I have tried using eosin to color the tissues > before processing but the color came out before paraffin. The coating on > the eggs will not absorb the dye. Does anyone have a suggestion for dyeing > or marking these tissues so I can see them better to embed. Thanks in > advance, > Cheryl > > Cheryl Crowder, BA, HTL(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From leila.etemadi <@t> med.lu.se Fri Jan 31 03:29:51 2014 From: leila.etemadi <@t> med.lu.se (Leila Etemadi) Date: Fri Jan 31 03:30:02 2014 Subject: [Histonet] Replacing VIP? Message-ID: <23B0E2C1-64E0-45DD-B0F7-AC6219045D91@med.lu.se> Hello there, I currently working with VIP-antirabbit marker to trace pain in PNS and CNS ( Alexa 488 secondary) in rat. Since it is expressing many areas in spinal cord and brain, I am running to huge difficulties to detect its up regulation with ordinary immune fluorescence techniques . I couldn?t find any colourful pictures in literatures also, that can help me to compare my results with others! Does any one has a suggestion for replacing my trouble maker with another antibody which is easier to detect? Cheers, Leila From MICHELLE.LAMPHERE <@t> childrens.com Fri Jan 31 07:35:21 2014 From: MICHELLE.LAMPHERE <@t> childrens.com (Michelle Lamphere) Date: Fri Jan 31 07:35:30 2014 Subject: [Histonet] RE: Dako's Omnis Message-ID: We are in the process of having the Omnis installed at our facility. We did a fairly extensive (side by side, as much as was possible) comparison of the Ventana Ultra, the Leica Bond, and the Dako Omnis instruments before deciding on the Omnis. I will be happy to share our experiences about the Omnis with you as we are discovering them. Michelle Lamphere, HT(ASCP) Lead Tech, Histology Department of Anatomic Pathology 1935 Medical District Dr. Dallas, TX 75235 214.456.2318 Message: 3 Date: Wed, 29 Jan 2014 17:17:16 -0800 From: joe joe Subject: [Histonet] Dako's Omnis To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Can anyone share their experiences with me about Dako's new IHC / FISH instrument? Thanks in advance! Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at privacy@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From abtdhu <@t> gmail.com Fri Jan 31 11:42:47 2014 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Fri Jan 31 11:42:53 2014 Subject: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone? Message-ID: which company do you ordered MMA? Sigma has back order, so I try to find reliable resource here. Thanks, Dorothy abtdhu@gmail.com From barbara.tibbs <@t> accuratediagnosticlabs.com Fri Jan 31 11:53:02 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Fri Jan 31 11:53:09 2014 Subject: [Histonet] marking tiny specimens In-Reply-To: <73a0afa0133b2.52eafaa6@uwo.ca> References: <2d61c62e716545ff9d623ea4194c7b5d@BL2PR04MB196.namprd04.prod.outlook.com> <7390834c14a59.52eb3f1d@uwo.ca> <73a0c4f116ca7.52eb3f5a@uwo.ca> <7400ee8d12ef6.52eb3f98@uwo.ca> <731083d91792c.52eb404e@uwo.ca> <7400e1fc16eb2.52eb408d@uwo.ca>,<73a0afa0133b2.52eafaa6@uwo.ca> Message-ID: <0fb82e590a0542fe881c91f431d3d505@BL2PR04MB196.namprd04.prod.outlook.com> Eyelashes are pointed at the end. Other body hair is not. I would re-use my tiny "paintbrush" over and over. No need to pull out an eyelash every day! Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________ From: John Kiernan Sent: Friday, January 31, 2014 4:21 AM To: Barbara Tibbs; Cheryl Crowder; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] marking tiny specimens Dedication to duty! Ouch! Did you ever run out of eyelashes? Why not a short eyebrow or forearm hair? JK = = = On 30/01/14, Barbara Tibbs wrote: Are you mixing Eosin in the last two alcohols of your processor? That should work. If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing. Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink. I would do this under a dissecting microscope. Maybe you could do that with the eggs? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cheryl Crowder Sent: Thursday, January 30, 2014 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking tiny specimens I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmlaud <@t> gmail.com Fri Jan 31 11:56:55 2014 From: dmlaud <@t> gmail.com (Damien Laudier) Date: Fri Jan 31 11:57:01 2014 Subject: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone? In-Reply-To: References: Message-ID: <5666506A-218C-453A-B159-457C603D4B6B@gmail.com> Hi Dorothy, I buy Methyl Methacrylate from Fisher Scientific. -Damien L. Sent from my iPhone > On Jan 31, 2014, at 12:42 PM, Dorothy Hu wrote: > > which company do you ordered MMA? > Sigma has back order, so I try to find reliable resource here. > > Thanks, > > Dorothy > abtdhu@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Fri Jan 31 12:46:08 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Jan 31 12:46:20 2014 Subject: [Histonet] Gross lab seniors Message-ID: We have several gross lab senior grossing stations that are vented outside. Our engineering asked today whether the airflow should be checked yearly like other exhaust hoods. Problem is there is not a door like other hoods of course and how would you measure the airflow? Recommended airflow is 500cfm however clearly the airflow at the working surface is not anything close to that. I wondered how anybody else monitors the gross lab seniors or do they at all. CAP used to ask about documentation for checking hoods however I can't recall them ever checking on grossing stations. We change filters annually only since they are vented outside. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From wdesalvo.cac <@t> outlook.com Fri Jan 31 13:12:34 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Fri Jan 31 13:12:40 2014 Subject: [Histonet] Gross lab seniors In-Reply-To: References: Message-ID: We use a company called C-Scan Technologies, Phoenix, AZ. The way they test all our gross dissection stations is by testing for directional or smoke containment and face velocity. We also check th they external pathway is clear and if the unit has a filtering system, the filters are changed regularly. The air flow measurement is Feet per minute (FPM) for face velocity and includes width, height, depth and total square ft for the working area. They exhaust flow in CFM. Face velocity minimum requirement is 100 fpm, exhaust flow requirement is >500 cfm. Face velocity fluctuates depending on the room and the air exchange rate for the area. I have always felt the face velocity is most important to gross dissection personnel. There needs to be adequate draw away from the employee, no matter the physical conditions of the room. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: Vickroy.Jim@mhsil.com > To: histonet@lists.utsouthwestern.edu > Date: Fri, 31 Jan 2014 12:46:08 -0600 > Subject: [Histonet] Gross lab seniors > > > We have several gross lab senior grossing stations that are vented outside. Our engineering asked today whether the airflow should be checked yearly like other exhaust hoods. Problem is there is not a door like other hoods of course and how would you measure the airflow? Recommended airflow is 500cfm however clearly the airflow at the working surface is not anything close to that. I wondered how anybody else monitors the gross lab seniors or do they at all. CAP used to ask about documentation for checking hoods however I can't recall them ever checking on grossing stations. We change filters annually only since they are vented outside. > > Jim > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ratliffjack <@t> hotmail.com Fri Jan 31 13:15:44 2014 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Fri Jan 31 13:15:49 2014 Subject: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone? In-Reply-To: <5666506A-218C-453A-B159-457C603D4B6B@gmail.com> References: , <5666506A-218C-453A-B159-457C603D4B6B@gmail.com> Message-ID: Dorothy, I personally order from Sigma and I have to tell you that in all my years of working with methyl methacrylate, they have been the most reliable source. I must admit that I did once use Fisher for a brief period of time due to pricing concerns from the purchasing department as there was something of an issue with the hazardous shipping from Sigma being almost as much as the MMA price, but not the case with Fisher. Honestly though I only used Fisher for about a year to a year and a half and switched back because of an issue with a particular lot that I encountered. During that particular issue my MMA blocks had polymerized with a sort of amber tint and with a pleasant orange fruity smell. It was alarming to me because I was completely used to having made crystal clear resin blocks and now I was starting to really love this orange smell during block preparation at the grinder that used to be a pungent MMA smell thats not really good for you under prolonged or excessive exposure. As time went by I then started to notice a change in polymerization rates and even sometimes an incomplete polymerization with a rubbery surface. While I did not recall any serious issue with staining, I did notice that cutting thin sections became a bit inconsistent and troublesome at times. It was then that I needed to discover if it was something that I was now doing differently than in the several years past of consistency or the chemicals that I was using. I ordered a new bottle off MMA from Sigma, changing only one variable, and ran a test. Turned out that I was back instantly to the clear blocks that I was accustomed to creating and that the issue had to be the Fisher bottle/lot of MMA. I want to point out that I am NOT saying that the Fisher MMA is an unreliable source of MMA. It was in my opinion clearly an issue of a bad lot/batch of product. However, given my OCD tendencies within the laboratory, I quickly reminded myself that "if it's not broke, don't fix it!" Basically, I shouldn't let pricing sacrifice the quality and consistency that I was accustomed to experiencing as I only switched suppliers due to a request for a cheaper alternative by the purchasing department. Oh and one more thing, I found out later that the orange smell was due to the presence of either solution or residue from the orange cleaning solution that the glass bottles are subjected to prior to filling with chemicals so like I said, could have been a one in a million experience but my mind told me I could not take any more chances! :) Best Regards, Jack > From: dmlaud@gmail.com > Date: Fri, 31 Jan 2014 12:56:55 -0500 > To: abtdhu@gmail.com > Subject: Re: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone? > CC: histonet@lists.utsouthwestern.edu > > Hi Dorothy, > > I buy Methyl Methacrylate from Fisher Scientific. > > -Damien L. > > Sent from my iPhone > > > On Jan 31, 2014, at 12:42 PM, Dorothy Hu wrote: > > > > which company do you ordered MMA? > > Sigma has back order, so I try to find reliable resource here. > > > > Thanks, > > > > Dorothy > > abtdhu@gmail.com > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert-Eytalis <@t> RiversideHealthCare.net Fri Jan 31 14:15:46 2014 From: Robert-Eytalis <@t> RiversideHealthCare.net (Eytalis, Robert A) Date: Fri Jan 31 14:15:53 2014 Subject: [Histonet] RE: Ventana LEAN team In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011245D90D@JERRY.Gia.com> References: , <5A33C952BB67F4468AF1F36D739212BC011245D90D@JERRY.Gia.com> Message-ID: Don't expect much. It is more of a way to market their products. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mckenzie@gastrodocs.net] Sent: Thursday, January 30, 2014 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LEAN team Has anyone had experience with Ventana's Lean team? I am correctly designing a new lab and they are coming out next tues to look at my workflow. Just wondering what to expect and how much they may change my current layout of my future lab :) Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis <@t> baylorhealth.edu Fri Jan 31 14:24:04 2014 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Fri Jan 31 14:24:10 2014 Subject: [Histonet] RE: Ventana LEAN team In-Reply-To: References: , <5A33C952BB67F4468AF1F36D739212BC011245D90D@JERRY.Gia.com> Message-ID: <2572B4D63B62E64A8078D8BBE34D407801A8EBE6@BHDASVEXML2.bhcs.pvt> I had a different experience with the workflow design team. A lot of good ideas. Good presentation provided to our upper management team. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Friday, January 31, 2014 2:16 PM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana LEAN team Don't expect much. It is more of a way to market their products. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 https://urldefense.proofpoint.com/v1/url?u=http://mail.riversidehealthcare.net/owa/redir.aspx?C%3DqIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.%26URL%3Dhttp%253A%252F%252Fwww.riversidemc.net%252F&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0A&s=ea1b568633a08afeb5425493a27a6db54a0d23bb8cb8243d5b8a243a2cf24fb4 | https://urldefense.proofpoint.com/v1/url?u=http://mail.riversidehealthcare.net/owa/redir.aspx?C%3DqIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.%26URL%3Dhttp%253A%252F%252Fwww.facebook.com%252FRiversideMC&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0A&s=d9259289ca3bda8d72d66f8c847c386ff6120657a75ddeb820dc3127051e2d8f ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mckenzie@gastrodocs.net] Sent: Thursday, January 30, 2014 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LEAN team Has anyone had experience with Ventana's Lean team? I am correctly designing a new lab and they are coming out next tues to look at my workflow. Just wondering what to expect and how much they may change my current layout of my future lab :) Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0A&s=0e52ddbc65dba9078e38143111a399ffecbd263d55ae80f465e8f5b4d87abfbc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0A&s=0e52ddbc65dba9078e38143111a399ffecbd263d55ae80f465e8f5b4d87abfbc ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From BZIMMERM <@t> gru.edu Fri Jan 31 14:25:26 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Jan 31 14:25:32 2014 Subject: [Histonet] HISTOPALOOZA April 25 - April 27 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF80CBE6@EX-MLB-03.ad.georgiahealth.edu> It's that time of the year again! The Georgia Society for Histotechnology will be having its annual symposium at The Lodge and Spa at Callaway Gardens located in Pine Mountain, Georgia. This is a Marriott property so you can expect the accommodations to be excellent. Please go to the GSH website and click on the link for the Lodge. www.histosearch.com/gsh In the coming weeks I'll be offering little tidbits of information regarding the list of speakers and topics. Of course, I plan to give you my spin on things, too. Shhh hehe Billie Zimmerman MT(ASCP)QIHC GSH Secretary From Robert-Eytalis <@t> RiversideHealthCare.net Fri Jan 31 14:36:19 2014 From: Robert-Eytalis <@t> RiversideHealthCare.net (Eytalis, Robert A) Date: Fri Jan 31 14:36:31 2014 Subject: [Histonet] RE: Ventana LEAN team In-Reply-To: <2572B4D63B62E64A8078D8BBE34D407801A8EBE6@BHDASVEXML2.bhcs.pvt> References: , <5A33C952BB67F4468AF1F36D739212BC011245D90D@JERRY.Gia.com> , <2572B4D63B62E64A8078D8BBE34D407801A8EBE6@BHDASVEXML2.bhcs.pvt> Message-ID: Whenever fresh eyes look at a process there can be new idea. I would have to agree. It's free. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: Willis, Donna G. [Donna.Willis@baylorhealth.edu] Sent: Friday, January 31, 2014 2:24 PM To: Eytalis, Robert A; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: Ventana LEAN team I had a different experience with the workflow design team. A lot of good ideas. Good presentation provided to our upper management team. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.willis@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Friday, January 31, 2014 2:16 PM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana LEAN team Don't expect much. It is more of a way to market their products. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 https://urldefense.proofpoint.com/v1/url?u=http://mail.riversidehealthcare.net/owa/redir.aspx?C%3DqIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.%26URL%3Dhttp%253A%252F%252Fwww.riversidemc.net%252F&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0A&s=ea1b568633a08afeb5425493a27a6db54a0d23bb8cb8243d5b8a243a2cf24fb4 | https://urldefense.proofpoint.com/v1/url?u=http://mail.riversidehealthcare.net/owa/redir.aspx?C%3DqIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.%26URL%3Dhttp%253A%252F%252Fwww.facebook.com%252FRiversideMC&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0A&s=d9259289ca3bda8d72d66f8c847c386ff6120657a75ddeb820dc3127051e2d8f ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mckenzie@gastrodocs.net] Sent: Thursday, January 30, 2014 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LEAN team Has anyone had experience with Ventana's Lean team? I am correctly designing a new lab and they are coming out next tues to look at my workflow. Just wondering what to expect and how much they may change my current layout of my future lab :) Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0A&s=0e52ddbc65dba9078e38143111a399ffecbd263d55ae80f465e8f5b4d87abfbc _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0A&r=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0A&m=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0A&s=0e52ddbc65dba9078e38143111a399ffecbd263d55ae80f465e8f5b4d87abfbc ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From dmlaud <@t> gmail.com Fri Jan 31 14:44:43 2014 From: dmlaud <@t> gmail.com (Damien Laudier) Date: Fri Jan 31 14:44:50 2014 Subject: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone? In-Reply-To: References: <5666506A-218C-453A-B159-457C603D4B6B@gmail.com> Message-ID: <1F08355D-1449-4B45-B6AC-B811D6D1DEAC@gmail.com> Oh,that's awful! Fortunately,I've never had a problem with product from either company;comparable results. -Damien Sent from my iPhone > On Jan 31, 2014, at 2:15 PM, Jack Ratliff wrote: > > Dorothy, > > I personally order from Sigma and I have to tell you that in all my years of working with methyl methacrylate, they have been the most reliable source. > > I must admit that I did once use Fisher for a brief period of time due to pricing concerns from the purchasing department as there was something of an issue with the hazardous shipping from Sigma being almost as much as the MMA price, but not the case with Fisher. Honestly though I only used Fisher for about a year to a year and a half and switched back because of an issue with a particular lot that I encountered. During that particular issue my MMA blocks had polymerized with a sort of amber tint and with a pleasant orange fruity smell. It was alarming to me because I was completely used to having made crystal clear resin blocks and now I was starting to really love this orange smell during block preparation at the grinder that used to be a pungent MMA smell thats not really good for you under prolonged or excessive exposure. As time went by I then started to notice a change in polymerization rates and even sometimes an incomplete polymerization with a rubbery surface. While I did not recall any serious issue with staining, I did notice that cutting thin sections became a bit inconsistent and troublesome at times. It was then that I needed to discover if it was something that I was now doing differently than in the several years past of consistency or the chemicals that I was using. I ordered a new bottle off MMA from Sigma, changing only one variable, and ran a test. Turned out that I was back instantly to the clear blocks that I was accustomed to creating and that the issue had to be the Fisher bottle/lot of MMA. > > I want to point out that I am NOT saying that the Fisher MMA is an unreliable source of MMA. It was in my opinion clearly an issue of a bad lot/batch of product. However, given my OCD tendencies within the laboratory, I quickly reminded myself that "if it's not broke, don't fix it!" Basically, I shouldn't let pricing sacrifice the quality and consistency that I was accustomed to experiencing as I only switched suppliers due to a request for a cheaper alternative by the purchasing department. Oh and one more thing, I found out later that the orange smell was due to the presence of either solution or residue from the orange cleaning solution that the glass bottles are subjected to prior to filling with chemicals so like I said, could have been a one in a million experience but my mind told me I could not take any more chances! :) > > Best Regards, > > Jack > > > > From: dmlaud@gmail.com > > Date: Fri, 31 Jan 2014 12:56:55 -0500 > > To: abtdhu@gmail.com > > Subject: Re: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone? > > CC: histonet@lists.utsouthwestern.edu > > > > Hi Dorothy, > > > > I buy Methyl Methacrylate from Fisher Scientific. > > > > -Damien L. > > > > Sent from my iPhone > > > > > On Jan 31, 2014, at 12:42 PM, Dorothy Hu wrote: > > > > > > which company do you ordered MMA? > > > Sigma has back order, so I try to find reliable resource here. > > > > > > Thanks, > > > > > > Dorothy > > > abtdhu@gmail.com > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet