[Histonet] Collagen 7
Caitlin Hesketh
caithesketh <@t> gmail.com
Thu Feb 20 11:00:14 CST 2014
Does anyone know of a lab that performs an IHC of this antibody, and will
do stains for other labs if we pay for it?
Thanks!
On Thursday, February 20, 2014, <histonet-request <@t> lists.utsouthwestern.edu>
wrote:
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> Today's Topics:
>
> 1. Re: aqueous mounting medium (Hans B Snyder)
> 2. Re: aqueous mounting medium (Caroline Miller)
> 3. Billing (Abbott, Tanya)
> 4. Re: aqueous mounting medium (Mehmet Fatih BOZKURT)
> 5. Used Equipment (McKenzie, Emily)
> 6. Job Opportunity at Georgetown University Histopathology and
> Tissue Shared Resource Lab (Anna Coffey)
> 7. Dried out liver bx's (SARAH GIBSON)
> 8. HIRING PT and per diem DELRAY BEACH FL
> (Delray Beach Pathology Kari Simeone)
> 9. Re: Dried out liver bx's (Mehmet Fatih BOZKURT)
> 10. Cytolyt Solution (Rolly Perez)
> 11. Mesothelioma Control (Coppin, Margaret)
> 12. RE: Cytolyt Solution (Walter Benton)
> 13. Validating New IHC antibodies (Abbott, Tanya)
> 14. affymetrix Anti-Mouse F4/80 antigen purified (Jennifer Leigh)
> 15. RE: Validating New IHC antibodies (joelle weaver)
> 16. RE: Dried out liver bx's (Tony Henwood (SCHN))
> 17. Xylene Resistant Labels (Schaundra Walton)
> 18. RE: Xylene Resistant Labels (Elizabeth Chlipala)
> 19. Re: Dried out liver bx's (Campbell, Tasha M.)
> 20. Re: aqueous mounting medium (Lee & Peggy Wenk)
> 21. Re: aqueous mounting medium (Jennifer MacDonald)
> 22. Re: Dried out liver bx's (Jennifer MacDonald)
> 23. RE: Corpora amylacea are destroyed after antigen retrieval?
> (Hoekert, W.E.J.)
> 24. Choosing a tissue processor (Md. Aminul Islam Khan)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 19 Feb 2014 13:11:41 -0500
> From: Hans B Snyder <hans <@t> histologistics.com>
> Subject: Re: [Histonet] aqueous mounting medium
> To: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <AFC32A5E-C9C7-49FC-AEA2-43E34823F99F <@t> histologistics.com>
> Content-Type: text/plain; charset=us-ascii
>
> Hello,
>
> I have one for ORO but not for IF. If someone has one for IF, I would be
> interested also.
>
> Thank you
>
> Histologistics
> Hans B Snyder
> 508.308.7800
> Hans <@t> histologistics.com
>
> > On Feb 19, 2014, at 12:27, Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> wrote:
> >
> > Does anyone have a good "recipe" for aqueous mounting medium?
> > Thanks,
> > Jennifer
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 19 Feb 2014 10:22:58 -0800
> From: Caroline Miller <cmiller <@t> gladstone.ucsf.edu>
> Subject: Re: [Histonet] aqueous mounting medium
> To: Hans B Snyder <hans <@t> histologistics.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>, Jennifer MacDonald
> <JMacDonald <@t> mtsac.edu>
> Message-ID: <BC346935-CEAB-42E1-ABB3-A5245808BA5B <@t> gladstone.ucsf.edu>
> Content-Type: text/plain; charset=us-ascii
>
> I use prolong gold from life tech (formerly molecular probes and now part
> of the world dominating thermofisher) without Dapi as I do that separately,
> for IF and aquaslip from American master tech for non IF aqueous mounting
>
> Good luck!
> Caroline
>
> Caroline Miller
> Gladstone Institutes
> www.gladstoneinstitutes.org
>
> Tel: 415 7342566
> Cell: 415 2187297
>
>
>
>
> > On Feb 19, 2014, at 10:11 AM, Hans B Snyder <hans <@t> histologistics.com>
> wrote:
> >
> > Hello,
> >
> > I have one for ORO but not for IF. If someone has one for IF, I would
> be interested also.
> >
> > Thank you
> >
> > Histologistics
> > Hans B Snyder
> > 508.308.7800
> > Hans <@t> histologistics.com
> >
> >> On Feb 19, 2014, at 12:27, Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> wrote:
> >>
> >> Does anyone have a good "recipe" for aqueous mounting medium?
> >> Thanks,
> >> Jennifer
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 19 Feb 2014 18:24:50 +0000
> From: "Abbott, Tanya" <TanyaAbbott <@t> catholichealth.net>
> Subject: [Histonet] Billing
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 852F7D2C14FB464D80E182B15DB138AF30637E73 <@t> CHIEX006.CHI.catholichealth.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone bill for a "technical component" for send outs? Meaning,
> billing for supplies, time it takes to process sendout/receive them back
> in, etc?
> Thanks!
>
> Tanya G. Abbott RT (CSMLS)
> Manager Technologist, Histology/Cytology
> St. Joseph Medical Center
> Reading, PA 19603-0316
> ph 610-378-2635
> fax 610-898-5871
> email: tanyaabbott <@t> catholichealth.net
>
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>
> ------------------------------
>
> Message: 4
> Date: Wed, 19 Feb 2014 20:38:17 +0200
> From: Mehmet Fatih BOZKURT <fbozkurt <@t> gmail.com>
> Subject: Re: [Histonet] aqueous mounting medium
> To: Caroline Miller <cmiller <@t> gladstone.ucsf.edu>,
> Histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CA+pSMGSBik29vtkaCg2AnZQ1u0js3=
> NktSU+hmsrxXW+_0auTg <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> I'm using modified protocol. I'm storing it at 4C.
>
>
> *Aqueous Mounting Medium (glycerol gelatin)*
>
>
> *Kisser's**Kaiser's**Glycerol jelly**Modified*Gelatine10 g40 g65 g5
> gDistilled
> water35 ml210 ml300 ml50 mlGlycerol (glycerin)30 ml250 ml100 ml50 mlPhenol
> (carbolic acid) 5 ml5 ml
>
>
>
>
>
> Mix gelatin and distilled water. Heat in 50 C oven until gelatin is
> dissolved (it takes about 30 minutes). Add glycerin and adjust pH to 7.0
> using 1N NaOH (to avoid fading of hematoxylin). Store at room temperature.
>
>
>
> Warm to 37-40 C before mounting. Note: Modified protocol works well.
>
>
>
> from ihcworld.com
>
>
>
>
>
> On Wed, Feb 19, 2014 at 8:22 PM, Caroline Miller <
> cmiller <@t> gladstone.ucsf.edu
> > wrote:
>
> > I use prolong gold from life tech (formerly molecular probes and now part
> > of the world dominating thermofisher) without Dapi as I do that
> separately,
> > for IF and aquaslip from American master tech for non IF aqueous mounting
> >
> > Good luck!
> > Caroline
> >
> > Caroline Miller
> > Gladstone Institutes
> > www.gladstoneinstitutes.org
> >
> > Tel: 415 7342566
> > Cell: 415 2187297
> >
> >
> >
> >
> > > On Feb 19, 2014, at 10:11 AM, Hans B Snyder <hans <@t> histologistics.com>
> > wrote:
> > >
> > > Hello,
> > >
> > > I have one for ORO but not for IF. If someone has one for IF, I would
> > be interested also.
> > >
> > > Thank you
> > >
> > > Histologistics
> > > Hans B Snyder
> > > 508.308.7800
> > > Hans <@t> histologistics.com
> > >
> > >> On Feb 19, 2014, at 12:27, Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
> > wrote:
> > >>
> > >> Does anyone have a good "recipe" for aqueous mounting medium?
> > >> Thanks,
> > >> Jennifer
> > >> _______________________________________________
> > >> Histonet mailing list
> > >> Histonet <@t> lists.utsouthwestern.edu
> > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --
> Asst. Prof. Dr. Mehmet Fatih BOZKURT
> Department of Pathology
> Faculty of Veterinary Medicine
> Afyon Kocatepe University
> 03030, ANS Campus
> Afyonkarahisar-TURKEY
> Tel: +902722281312-16173/16237
>
> Asst. Prof. Dr. Mehmet Fatih BOZKURT
> Manager of Veterinary Diagnostic Laboratory
> Afyon Kocatepe University
> 03030, ANS Campus
> Afyonkarahisar-TURKEY
> Tel: +902722281312-16300-16301
> vetanaliz <@t> aku.edu.tr
>
>
> ------------------------------
>
> Message: 5
> Date: Wed, 19 Feb 2014 12:46:17 -0600
> From: "McKenzie, Emily" <McKenzie.Emily <@t> mhsil.com>
> Subject: [Histonet] Used Equipment
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <FCDCEC5C9D3DEF479965D2EF67FEB2A4A933BCD4 <@t> mmc-mail.ad.mhsil.com>
> Content-Type: text/plain; charset="koi8-r"
>
> Hello Histoneters!!
> Our facility is upgrading our IHC process. We are currently in the market
> for a used linear stainer (for hydrate and dehydrate) and/or a used
> coverslipper (glass). These items need to be fairly small since we don't
> have a lot of room to put equipment. If there are any facilities out there
> that have either of these and are willing to part with them please let me
> know.
> If you are not interested in parting with used equipment can someone
> suggest small linear strainers and small coverslipper's that aren't too
> expensive.
> Thank you for your help!!!
>
> Emily K. McKenzie BS, HT(ASCP)
>
> Memorial Medical Center 701 North First Street Springfield, IL 62781
> Ph: 217-788-3991 email: McKenzie.Emily <@t> mhsil.com
>
>
>
>
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>
> ------------------------------
>
> Message: 6
> Date: Wed, 19 Feb 2014 14:34:17 -0500
> From: Anna Coffey <ahc53 <@t> georgetown.edu>
> Subject: [Histonet] Job Opportunity at Georgetown University
> Histopathology and Tissue Shared Resource Lab
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <
> CALVW9z6-+AEYqNUQdkiEdA6TmNKY569wNh_C3ZvrdpVaasUxdg <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> We have a new histology technician position that has just opened in the
> Histopathology and Tissue Shared Resource lab at Georgetown University's
> Lombardi Comprehensive Cancer Center. We're looking for someone with some
> lab experience (B.S. in biology, chemistry, or related field preferred) who
> is strongly motivated to gain more specialized histology experience,
> specifically with microtomy. This would be an ideal position for someone
> looking to fill their experience requirements for their HT/HTL
> certification. We are hoping to fill this position as quickly as possible.
> Please contact me with any questions.
>
> IMPORTANT: All interested applicants should submit applications directly
> through this website -
>
> http://www12.georgetown.edu/hr/employment_services/joblist/job_description.cfm?CategoryID=7&RequestNo=20140338
>
> --
> Anna Coffey
> Senior Histology Technician
> Department of Oncology
> Histopathology and Tissue Shared Resource
> LR-10 Pre-Clinical Sciences Building
> Lombardi Comprehensive Cancer Center
> Georgetown University
> 202-687-7890
> ahc53 <@t> georgetown.edu
>
>
> ------------------------------
>
> Message: 7
> Date: Wed, 19 Feb 2014 13:46:25 -0600
>
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