[Histonet] RE: Mouse F4/80 antibody

[Elizabeth Chlipala]

I'll chime in here and state that we have used F4/80, iba-1, and CD68 for mouse macrophages, for CD68 we use the same clone as Ray is talking about we get that particular antibody from Serotec.  All work on FFPE tissues in our hands.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of koellingr <@t> comcast.net
Sent: Wednesday, February 12, 2014 2:51 PM
To: pruegg <@t> ihctech.net
Cc: histonet <@t> lists.utsouthwestern.edu; Anna Hughes
Subject: Re: [Histonet] RE: Mouse F4/80 antibody

Hello, I agree anti-human CD68 might not be best for mouse tissue but there are specific mouse CD68 (the mouse homolog to human CD68) antibodies out there.  FA-11 is one of my favorites.  Have used both F4/80 and (rat anti-mouse CD68) on flow, frozens and FFPE.  Anna, a literature search gives you published  protocols.  I'm looking right now at a photo of some CD68+ macs staining on mouse tissues I took from years back.  Shows them nicely.  anti-mouse CD68 on mouse tissue is in the literature. 
  
Ray
Seattle 

----- Original Message -----

From: pruegg <@t> ihctech.net
To: "Anna Hughes" <anna.c.hughes <@t> gsk.com>, histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, February 12, 2014 12:02:07 PM
Subject: [Histonet] RE: Mouse F4/80 antibody 

Rat anti ms F4/80 is used on mouse tissue ffpe for macrophages not anti human cd68. 

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anna Hughes
Sent: Wednesday, February 12, 2014 9:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mouse F4/80 antibody 

Hi Everyone! 
I was wondering if any of you have a favorite CD68 macrophage antibody that you have used on mouse tissue and is especially reliable.  This is a target that has been notorious in our lab and I was wondering if there was a really good one to use. 

Thanks in advance for your help! 

Anna Hughes
Anna C. Hughes, BBA, BS, HTLCM
anna.c.hughes <@t> gsk.com 


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