From ewj <@t> pigsqq.org Sat Feb 1 16:12:35 2014 From: ewj <@t> pigsqq.org (=?UTF-8?B?IkUuIFdheW5lIEpvaG5zb24g5pyx56iz5qOuIg==?=) Date: Sat Feb 1 16:12:51 2014 Subject: [Histonet] Gross lab seniors In-Reply-To: References: Message-ID: <52ED7153.4030605@pigsqq.org> Face velocity is simply the airflow rate in CFM divided by the area of the hood opening in square feet. A smaller opening at the same flow rate gives a higher face velocity. Titanium tetrachloride in a small plastic squeeze bottle can be used to generate "smoke". On 3:59 AM, WILLIAM DESALVO wrote: > We use a company called C-Scan Technologies, Phoenix, AZ. The way they test all our gross dissection stations is by testing for directional or smoke containment and face velocity. We also check th they external pathway is clear and if the unit has a filtering system, the filters are changed regularly. The air flow measurement is Feet per minute (FPM) for face velocity and includes width, height, depth and total square ft for the working area. They exhaust flow in CFM. Face velocity minimum requirement is 100 fpm, exhaust flow requirement is>500 cfm. Face velocity fluctuates depending on the room and the air exchange rate for the area. I have always felt the face velocity is most important to gross dissection personnel. There needs to be adequate draw away from the employee, no matter the physical conditions of the room. > > William DeSalvo, BS HTL(ASCP) > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > Owner/Consultant, Collaborative Advantage Consulting > > > >> From: Vickroy.Jim@mhsil.com >> To: histonet@lists.utsouthwestern.edu >> Date: Fri, 31 Jan 2014 12:46:08 -0600 >> Subject: [Histonet] Gross lab seniors >> >> >> We have several gross lab senior grossing stations that are vented outside. Our engineering asked today whether the airflow should be checked yearly like other exhaust hoods. Problem is there is not a door like other hoods of course and how would you measure the airflow? Recommended airflow is 500cfm however clearly the airflow at the working surface is not anything close to that. I wondered how anybody else monitors the gross lab seniors or do they at all. CAP used to ask about documentation for checking hoods however I can't recall them ever checking on grossing stations. We change filters annually only since they are vented outside. >> >> Jim >> >> James Vickroy BS, HT(ASCP) >> >> Surgical and Autopsy Pathology Technical Supervisor >> Memorial Medical Center >> 217-788-4046 >> >> >> ________________________________ >> This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From blayjorge <@t> gmail.com Sat Feb 1 17:00:04 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Sat Feb 1 17:00:09 2014 Subject: [Histonet] protocols to fix insect wings that are quite hirsute Message-ID: Hello: Can someone point me on the direction of protocols to fix insect wings that are quite hirsute. I would like to increase the likelihood of the preservative actually going through the carpet of setae to actually fix the interior of the wing. Thanks for any help. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From ratliffjack <@t> hotmail.com Sun Feb 2 09:45:19 2014 From: ratliffjack <@t> hotmail.com (Jack Ratliff) Date: Sun Feb 2 09:45:29 2014 Subject: [Histonet] protocols to fix insect wings that are quite hirsute In-Reply-To: References: Message-ID: This sounds like a question for the insect histology expert, Damien Laudier! He monitors this site so I am sure he will respond to you privately. :) Jack > On Feb 1, 2014, at 5:00 PM, "Jorge A. Santiago-Blay" wrote: > > Hello: > > Can someone point me on the direction of protocols to fix insect wings that > are quite hirsute. I would like to increase the likelihood of the > preservative actually going through the carpet of setae to actually fix the > interior of the wing. Thanks for any help. > > Sincerely, > > Jorge > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > http://blayjorge.wordpress.com/ > http://paleobiology.si.edu/staff/individuals/santiagoblay.html > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wdesalvo.cac <@t> outlook.com Sun Feb 2 12:34:15 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Sun Feb 2 12:37:42 2014 Subject: =?utf-8?Q?Re:_[Histonet]_Gross_lab_seniors?= Message-ID: Absolutely the Face velocity changes with the work area set-up. This is why I like to make sure the air flow away is maintained at a minimum. Most grossing stations have large working area and often the flow away from the grosser is not checked, just the vent opening draw. Most important is to set up a process and then regularly check. Sent from Windows Mail From: E. Wayne Johnson ??? Sent: ?Saturday?, ?February? ?1?, ?2014 ?3?:?12? ?PM To: WILLIAM DESALVO Cc: Vickroy, Jim, histonet Face velocity is simply the airflow rate in CFM divided by the area of the hood opening in square feet. A smaller opening at the same flow rate gives a higher face velocity. Titanium tetrachloride in a small plastic squeeze bottle can be used to generate "smoke". On 3:59 AM, WILLIAM DESALVO wrote: > We use a company called C-Scan Technologies, Phoenix, AZ. The way they test all our gross dissection stations is by testing for directional or smoke containment and face velocity. We also check th they external pathway is clear and if the unit has a filtering system, the filters are changed regularly. The air flow measurement is Feet per minute (FPM) for face velocity and includes width, height, depth and total square ft for the working area. They exhaust flow in CFM. Face velocity minimum requirement is 100 fpm, exhaust flow requirement is>500 cfm. Face velocity fluctuates depending on the room and the air exchange rate for the area. I have always felt the face velocity is most important to gross dissection personnel. There needs to be adequate draw away from the employee, no matter the physical conditions of the room. > > William DeSalvo, BS HTL(ASCP) > Production Manager-Anatomic Pathology > Chair, NSH Quality Management Committee > Owner/Consultant, Collaborative Advantage Consulting > > > >> From: Vickroy.Jim@mhsil.com >> To: histonet@lists.utsouthwestern.edu >> Date: Fri, 31 Jan 2014 12:46:08 -0600 >> Subject: [Histonet] Gross lab seniors >> >> >> We have several gross lab senior grossing stations that are vented outside. Our engineering asked today whether the airflow should be checked yearly like other exhaust hoods. Problem is there is not a door like other hoods of course and how would you measure the airflow? Recommended airflow is 500cfm however clearly the airflow at the working surface is not anything close to that. I wondered how anybody else monitors the gross lab seniors or do they at all. CAP used to ask about documentation for checking hoods however I can't recall them ever checking on grossing stations. We change filters annually only since they are vented outside. >> >> Jim >> >> James Vickroy BS, HT(ASCP) >> >> Surgical and Autopsy Pathology Technical Supervisor >> Memorial Medical Center >> 217-788-4046 >> >> >> ________________________________ >> This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From dmlaud <@t> laudierhistology.com Mon Feb 3 04:40:33 2014 From: dmlaud <@t> laudierhistology.com (Damien) Date: Mon Feb 3 04:40:37 2014 Subject: [Histonet] protocols to fix insect wings that are quite hirsute In-Reply-To: References: Message-ID: Hi Jorge, I'd be happy to help you if I can. Please feel free to message me. Best, Damien L On Sun, Feb 2, 2014 at 10:45 AM, Jack Ratliff wrote: > This sounds like a question for the insect histology expert, Damien > Laudier! He monitors this site so I am sure he will respond to you > privately. :) > > Jack > > > On Feb 1, 2014, at 5:00 PM, "Jorge A. Santiago-Blay" < > blayjorge@gmail.com> wrote: > > > > Hello: > > > > Can someone point me on the direction of protocols to fix insect wings > that > > are quite hirsute. I would like to increase the likelihood of the > > preservative actually going through the carpet of setae to actually fix > the > > interior of the wing. Thanks for any help. > > > > Sincerely, > > > > Jorge > > > > Jorge A. Santiago-Blay, PhD > > blaypublishers.com > > http://blayjorge.wordpress.com/ > > http://paleobiology.si.edu/staff/individuals/santiagoblay.html > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Damien Laudier Laudier Histology www.LaudierHistology.com From thigginsht <@t> msn.com Mon Feb 3 07:58:51 2014 From: thigginsht <@t> msn.com (Tim Higgins) Date: Mon Feb 3 07:58:55 2014 Subject: [Histonet] RE: Ventana LEAN In-Reply-To: References: Message-ID: They come in, give their opinions on how to make your workflow better. I didn't get much from it, we didn't make any changes, more of a selling tool in my opion. I guess if your workflow was terrible, they might be able to offer good advice. On a positive, they were all very nice. Thank, Tim H. From trathborne <@t> somerset-healthcare.com Mon Feb 3 08:32:59 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Mon Feb 3 08:33:23 2014 Subject: [Histonet] RE: Ventana LEAN In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95B53C7@SMCMAIL01.somerset-healthcare.com> Another benefit of someone coming in from the outside (Leica did the same for us), is that since this is what these people do every day, management is more likely to listen to their suggestions. If it's the same as yours then that would reinforce your current plans. If it's different than what you had in mind, they will generally try to help with what you want to do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Monday, February 03, 2014 8:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana LEAN They come in, give their opinions on how to make your workflow better. I didn't get much from it, we didn't make any changes, more of a selling tool in my opion. I guess if your workflow was terrible, they might be able to offer good advice. On a positive, they were all very nice. Thank, Tim H. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans <@t> histologistics.com Mon Feb 3 08:53:45 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Mon Feb 3 08:53:50 2014 Subject: [Histonet] How to De-stain trichrome Message-ID: Does anyone know how to destain Massons trichrome? Is it possible? Thank you Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com From nicole <@t> dlcjax.com Mon Feb 3 09:19:07 2014 From: nicole <@t> dlcjax.com (nicole@dlcjax.com) Date: Mon Feb 3 09:19:13 2014 Subject: [Histonet] Histotech position FT West Palm Beach Message-ID: <1940731504.67335.1391440747343.JavaMail.mail@webmail17> Posting for I/MD Path labs West Palm Beach, Florida Looking for full time Histotech. Dermpath lab. Please contact Dr. Morales. 1(561)653-8005 From c.tague <@t> Pathologyarts.com Mon Feb 3 11:06:56 2014 From: c.tague <@t> Pathologyarts.com (Curt) Date: Mon Feb 3 09:59:46 2014 Subject: [Histonet] anyone need a glass cover slipper Message-ID: <9C8F910F72893643B3C3793C3D67132B01440853@PATHOLOGYSERVER.pathologyarts.local> We have on old Hacker glass cover slipper, I think it's a Hacker.... The name plate on the front says Meisei but I'm told it's a Hacker. Anyway, the model number: RCM-3660 if that's helpful. I'm not looking to make a bunch of money, so many people here have been helpful over the years from knowledge and advice to helping me out with control tissue. If someone needs one, I'd be willing to help out a bit, just need to cover the expense of shipping and packing. Let me know, I can snap a picture if desired. We recently had is serviced and it's said to be working fine by the technician. Curt From jcox90 <@t> yahoo.com Mon Feb 3 10:58:56 2014 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Mon Feb 3 10:59:10 2014 Subject: [Histonet] On-call position for Histology Assistant in Phoenix AZ Message-ID: <1391446736.89252.YahooMailBasic@web160701.mail.bf1.yahoo.com> Hi Netters, We are looking for an on-call histology assistant to cover for vacations and sick leave, already have a few days in March/April. We are a small Dermatology lab in NW Peoria AZ, great working conditions and people. Very nice place to work. Hours are from 7:30-4:00PM when needed. Email me for more details. Thank you, Jill Arrowhead Dermatology From Robert-Eytalis <@t> RiversideHealthCare.net Mon Feb 3 11:00:28 2014 From: Robert-Eytalis <@t> RiversideHealthCare.net (Eytalis, Robert A) Date: Mon Feb 3 11:00:32 2014 Subject: [Histonet] RE: Ventana LEAN In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95B53C7@SMCMAIL01.somerset-healthcare.com> References: , <3AD061FE740D464FAC7BF6B5CFB75707A95B53C7@SMCMAIL01.somerset-healthcare.com> Message-ID: Depends on if your management is skeptical of vendors. Mine automatically assume that they are marketing to us. Robert A. Eytalis Laboratory Manager robert-eytalis@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Rathborne, Toni [trathborne@somerset-healthcare.com] Sent: Monday, February 03, 2014 8:32 AM To: 'Tim Higgins'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Ventana LEAN Another benefit of someone coming in from the outside (Leica did the same for us), is that since this is what these people do every day, management is more likely to listen to their suggestions. If it's the same as yours then that would reinforce your current plans. If it's different than what you had in mind, they will generally try to help with what you want to do. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins Sent: Monday, February 03, 2014 8:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana LEAN They come in, give their opinions on how to make your workflow better. I didn't get much from it, we didn't make any changes, more of a selling tool in my opion. I guess if your workflow was terrible, they might be able to offer good advice. On a positive, they were all very nice. Thank, Tim H. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drbugge <@t> gmail.com Mon Feb 3 11:43:06 2014 From: drbugge <@t> gmail.com (Dawn Bugge) Date: Mon Feb 3 11:43:11 2014 Subject: [Histonet] Turn Around Time Message-ID: Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology From TMcNemar <@t> lmhealth.org Mon Feb 3 11:49:14 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Feb 3 11:49:21 2014 Subject: [Histonet] Turn Around Time In-Reply-To: References: Message-ID: Our benchmark is 24 hour TAT for 80% of cases. We provide same-day service for recuts and most in-house special stains. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From joelleweaver <@t> hotmail.com Mon Feb 3 11:56:47 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Feb 3 11:56:52 2014 Subject: [Histonet] Turn Around Time In-Reply-To: References: , Message-ID: ditto. Only FISH is allowed to take longer Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TMcNemar@lmhealth.org > To: drbugge@gmail.com; histonet@lists.utsouthwestern.edu > Date: Mon, 3 Feb 2014 12:49:14 -0500 > Subject: RE: [Histonet] Turn Around Time > CC: > > Our benchmark is 24 hour TAT for 80% of cases. We provide same-day service for recuts and most in-house special stains. > > Tom McNemar, HT(ASCP) > Histology Supervisor > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge > Sent: Monday, February 03, 2014 12:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Turn Around Time > > Hello Histonet > > I am just curious what the standard for Turn Around Time is for most labs. > I think a two day turn around time from the time the biopsy gets to the lab > to the time the pathologist signs out a case is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Mon Feb 3 12:03:28 2014 From: thisisann <@t> aol.com (Ann Specian) Date: Mon Feb 3 12:03:32 2014 Subject: [Histonet] Price for preparing IHC slides Message-ID: <8D0EF215FBFEBF2-1E68-1A6E4@webmail-m233.sysops.aol.com> Can anyone tell me the average cost for preparing an IHC slide? thanks, Ann From chapcl <@t> yahoo.com Mon Feb 3 12:08:28 2014 From: chapcl <@t> yahoo.com (Will Chappell) Date: Mon Feb 3 12:08:39 2014 Subject: [Histonet] Price for preparing IHC slides In-Reply-To: <8D0EF215FBFEBF2-1E68-1A6E4@webmail-m233.sysops.aol.com> References: <8D0EF215FBFEBF2-1E68-1A6E4@webmail-m233.sysops.aol.com> Message-ID: <77A77586-CF08-42B0-BABA-B972F803EA17@yahoo.com> A lab I used in Southern California charged $35 per stain for most antibodies. Sent from my iPhone > On Feb 3, 2014, at 1:03 PM, Ann Specian wrote: > > > Can anyone tell me the average cost for preparing an IHC slide? > thanks, Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From VRimkunas <@t> merrimackpharma.com Mon Feb 3 12:29:18 2014 From: VRimkunas <@t> merrimackpharma.com (Victoria Rimkunas) Date: Mon Feb 3 12:29:17 2014 Subject: [Histonet] IHC cartilage Message-ID: <2661A06F49EDCF41ABFEE80C2727C2237204B5A6@MEP-MBX2.mack.local> Does anyone have experience or a protocol for IHC staining on cartilage? From jaylundgren <@t> gmail.com Mon Feb 3 12:31:44 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Mon Feb 3 12:31:49 2014 Subject: [Histonet] Turn Around Time In-Reply-To: References: Message-ID: Yesterday. Sincerely? Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Feb 3, 2014 at 11:56 AM, joelle weaver wrote: > ditto. Only FISH is allowed to take longer > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: TMcNemar@lmhealth.org > > To: drbugge@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Mon, 3 Feb 2014 12:49:14 -0500 > > Subject: RE: [Histonet] Turn Around Time > > CC: > > > > Our benchmark is 24 hour TAT for 80% of cases. We provide same-day > service for recuts and most in-house special stains. > > > > Tom McNemar, HT(ASCP) > > Histology Supervisor > > Licking Memorial Health Systems > > (740) 348-4163 > > (740) 348-4166 > > tmcnemar@lmhealth.org > > www.LMHealth.org > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge > > Sent: Monday, February 03, 2014 12:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Turn Around Time > > > > Hello Histonet > > > > I am just curious what the standard for Turn Around Time is for most > labs. > > I think a two day turn around time from the time the biopsy gets to the > lab > > to the time the pathologist signs out a case is pretty fast. > > > > Thanks for your input. > > -- > > Dawn R Bugge > > Seattle Histology > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brett_connolly <@t> merck.com Mon Feb 3 12:35:22 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon Feb 3 12:35:27 2014 Subject: [Histonet] dust-free coverslips - do they really exist? Message-ID: What's your favorite? It gets frustrating when I need to capture low power images and there coverslips are dirty right out of the box. Fisherfinest Premium isn't bad, but I am wondering if there is something better??? Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rjbuesa <@t> yahoo.com Mon Feb 3 12:38:14 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 3 12:38:19 2014 Subject: [Histonet] Turn Around Time In-Reply-To: References: Message-ID: <1391452694.39779.YahooMailNeo@web120405.mail.ne1.yahoo.com> In the US the "standard" (or most frequent) TAT is 24 hours. In other countries the TAT varies greatly, in extreme cases up to 1 week for surgicals! Ren? J. ________________________________ From: Tom McNemar To: 'Dawn Bugge' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 3, 2014 12:49 PM Subject: RE: [Histonet] Turn Around Time Our benchmark is 24 hour TAT for 80% of cases.? We provide same-day service for recuts and most in-house special stains. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joewalker <@t> rrmc.org Mon Feb 3 12:43:49 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Mon Feb 3 12:43:56 2014 Subject: [Histonet] Price for preparing IHC slides In-Reply-To: <8D0EF215FBFEBF2-1E68-1A6E4@webmail-m233.sysops.aol.com> References: <8D0EF215FBFEBF2-1E68-1A6E4@webmail-m233.sysops.aol.com> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC181D2DCB@RRMBX03.rrmc.local> I believe that this would be highly dependent on the antibodies used, how often the antibodies are used, slide costs, tech costs, overhead, etc. What would be average for 1 institution might be above or below average for another. What are you trying to determine, Ann? Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Monday, February 03, 2014 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Price for preparing IHC slides Can anyone tell me the average cost for preparing an IHC slide? thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From michael <@t> mcevoyandfarmer.com Mon Feb 3 12:59:32 2014 From: michael <@t> mcevoyandfarmer.com (Michael Farmer) Date: Mon Feb 3 12:59:43 2014 Subject: [Histonet] Price for preparing IHC slides In-Reply-To: <8D0EF215FBFEBF2-1E68-1A6E4@webmail-m233.sysops.aol.com> References: <8D0EF215FBFEBF2-1E68-1A6E4@webmail-m233.sysops.aol.com> Message-ID: <35E9AD8D-1846-430D-BD59-71BB57DC7194@mcevoyandfarmer.com> This is a fascinating question, Ann - I've been studying this topic for the last couple of years in five countries. While I do not yet understand this complex phenomenon as well as I would wish to, these are my impressions about life here in the US. It is a ridiculous tale, but I will tell it to you... The smartest shoppers who have the biggest contracts (you can guess who those might be) are paying $5-10 for their highest-volume slides - ER, PR, HER-2 and a few others - but more like $10-15 for most of their menus. The smallest IHC customers think they are paying 20-something per slide, but they are actually paying $30-$40 per slide - and more in many cases. How this discrepancy? Two reasons: first, because the suppliers (I can't quite remember their names right now, please pardon my senior moment) are highly-skilled at making their price lists and service contracts as eye-glazingly complicated as possible. And second, because immutable human nature compels many mere mortals to underestimate their costs, particularly while they are still trying to rationalize a bad investment they made some time ago I'm pretty sure that American labs (excluding Canada, mind you) spent $700-750m with the IHC companies in 2013. I think that between 28m and 32 million IHC slides were run last year in the US. If you want to slice those estimates down the middle you'll come up with maybe $24/slide, once every penny of waste, service, and sub-optimal operating procedures are truly accounted for. There you have one of the most useless averages you'll ever hear. There are plenty of contracts out there in vast middle America at every price point between $7 and $30 per slide. You pay what your volume earns you, unless of course you happen to be in the market for a tissue processor or primary stainer at the time you're haggling with the IHC companies. I'll remember their names if you give me another a minute. That's the way it was last year. WIth these new codes, lots of small labs will be priced out of the IHC business by summertime, and the big labs that remain will have negotiated prices within a narrower range. That'll be a fast-moving target. Since I didn't see you in the crowd at the funeral of 88342 last month, I am attaching below our chronicle of the event. I'm always happy to banter about this with anyone who thinks the topic is interesting. Sincerely, Michael Farmer McEvoy & Farmer Pathology www.mcevoyandfarmer-pathology.com 415-994-8852 "Those who seek the truth doubt those who find it" - Andr? Gide -------------- next part -------------- On Feb 3, 2014, at 1:03 PM, Ann Specian wrote: > > Can anyone tell me the average cost for preparing an IHC slide? > thanks, Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Mon Feb 3 13:05:47 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Mon Feb 3 13:05:57 2014 Subject: [Histonet] Turn Around Time In-Reply-To: References: Message-ID: While I recognize the need for a quick result in some cases, I also subscribe to the theory that "speed kills". I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience. However that is another discussion altogether. That said, our institution shoots for the 80% in 24 hours as well. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Mon Feb 3 13:50:45 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Feb 3 13:50:48 2014 Subject: [Histonet] Re: Turn Around Time Message-ID: Dawn R. Bugge at Seattle WA Histology asks: >>I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast.<< Normally I'd receive the specimen on Monday and gross it, have it processed overnight, get the slides some time Tuesday morning, and sign the case out well before close of business on Tuesday. Exceptions are when the specimen needs to fix overnight before I gross it (bowel resection) or after I gross it (fatty breast tissue, amputation specimens, autopsies), or when a specimen of more than minimal size (or received after 1400) requires decalcification. What I say goes. Bob Richmond Samurai Pathologist Maryville TN From joewalker <@t> rrmc.org Mon Feb 3 13:54:21 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Mon Feb 3 13:54:28 2014 Subject: [Histonet] Turn Around Time In-Reply-To: References: Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC181D4260@RRMBX03.rrmc.local> I agree with you, Martha. That is why we look at it from an average of all cases for the month. We definitely encounter cases that are difficult and require more time with our pathologists. We track the TAT for statistical purposes only. The CAP (for those who are CAP accredited) has dropped this from their checklist for surgical cases. It seems to only really apply to autopsy cases now but these has a much different TAT target. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Monday, February 03, 2014 2:06 PM To: Dawn Bugge; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Turn Around Time While I recognize the need for a quick result in some cases, I also subscribe to the theory that "speed kills". I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience. However that is another discussion altogether. That said, our institution shoots for the 80% in 24 hours as well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From rjbuesa <@t> yahoo.com Mon Feb 3 15:37:49 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 3 15:37:53 2014 Subject: [Histonet] Turn Around Time In-Reply-To: References: Message-ID: <1391463469.21418.YahooMailNeo@web120406.mail.ne1.yahoo.com> Martha: So, do you mean that you are not satisfied with the quality of your slides? If you are, I just do not understand your concerns about a 24 h TAT! The fundamental issue is to?develop adequate protocols assuring quality as well as a?convenient (24 h) TAT. Just my 3 cents (after inflation!) Ren? J. ________________________________ From: Martha Ward-Pathology To: Dawn Bugge ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 3, 2014 2:05 PM Subject: RE: [Histonet] Turn Around Time While I recognize the need for a quick result in some cases, I also subscribe to the theory that "speed kills".? I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience.? ? However that is another discussion altogether.? That said, our institution shoots for the 80% in 24 hours as well. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Feb 3 15:58:51 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Feb 3 15:59:09 2014 Subject: [Histonet] How to De-stain trichrome In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00AD9EE@xmdb04.nch.kids> Hi Hans, It is possible, though it will depend on what you want to do to the slides afterword. The problem arises from the use of phosphotungstic or phosphmolybdic acid which is used for differentiation and mordanting in the trichrome stains like Masson's. These are very difficult to reverse (anyone trying to restain PAP smears might know the problem). This may cause problems. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: Tuesday, 4 February 2014 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to De-stain trichrome Does anyone know how to destain Massons trichrome? Is it possible? Thank you Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From madeleinehuey <@t> gmail.com Mon Feb 3 16:26:00 2014 From: madeleinehuey <@t> gmail.com (Madeleine Huey) Date: Mon Feb 3 16:26:07 2014 Subject: [Histonet] Re: Histonet Digest, Vol 123, Issue 3 In-Reply-To: <52efd959.262c3c0a.0306.ffffb20bSMTPIN_ADDED_MISSING@mx.google.com> References: <52efd959.262c3c0a.0306.ffffb20bSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Dawn, Our TAT is 94% with 24 hours & 98% within 48 hours (~ 6% complicated cases need more IPOX/Molecular Study/FACS & etc.). Madeleine Superviser - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org From: histonet-bounces@lists.utsouthwestern.edu [mailto: histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge > Sent: Monday, February 03, 2014 12:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Turn Around Time > > Hello Histonet > > I am just curious what the standard for Turn Around Time is for most labs. > I think a two day turn around time from the time the biopsy gets to the lab > to the time the pathologist signs out a case is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology On Mon, Feb 3, 2014 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Gross lab seniors (WILLIAM DESALVO) > 2. Re: protocols to fix insect wings that are quite hirsute (Damien) > 3. RE: Ventana LEAN (Tim Higgins) > 4. RE: RE: Ventana LEAN (Rathborne, Toni) > 5. How to De-stain trichrome (Hans B Snyder) > 6. Histotech position FT West Palm Beach (nicole@dlcjax.com) > 7. anyone need a glass cover slipper (Curt) > 8. On-call position for Histology Assistant in Phoenix AZ (Jill Cox) > 9. RE: RE: Ventana LEAN (Eytalis, Robert A) > 10. Turn Around Time (Dawn Bugge) > 11. RE: Turn Around Time (Tom McNemar) > 12. RE: Turn Around Time (joelle weaver) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 2 Feb 2014 18:34:15 +0000 > From: WILLIAM DESALVO > Subject: Re: [Histonet] Gross lab seniors > To: E. Wayne Johnson ??? > Cc: histonet , " Vickroy, Jim " > > Message-ID: > Content-Type: text/plain; charset="utf-8" > > Absolutely the Face velocity changes with the work area set-up. This is > why I like to make sure the air flow away is maintained at a minimum. Most > grossing stations have large working area and often the flow away from the > grosser is not checked, just the vent opening draw. Most important is to > set up a process and then regularly check. > > > > > > > Sent from Windows Mail > > > > > > From: E. Wayne Johnson ??? > Sent: ?Saturday?, ?February? ?1?, ?2014 ?3?:?12? ?PM > To: WILLIAM DESALVO > Cc: Vickroy, Jim, histonet > > > > > > Face velocity is simply the airflow rate in CFM divided by the area of > the hood opening in square feet. > > A smaller opening at the same flow rate gives a higher face velocity. > > Titanium tetrachloride in a small plastic squeeze bottle can be used to > generate "smoke". > > > On 3:59 AM, WILLIAM DESALVO wrote: > > We use a company called C-Scan Technologies, Phoenix, AZ. The way they > test all our gross dissection stations is by testing for directional or > smoke containment and face velocity. We also check th they external pathway > is clear and if the unit has a filtering system, the filters are changed > regularly. The air flow measurement is Feet per minute (FPM) for face > velocity and includes width, height, depth and total square ft for the > working area. They exhaust flow in CFM. Face velocity minimum requirement > is 100 fpm, exhaust flow requirement is>500 cfm. Face velocity fluctuates > depending on the room and the air exchange rate for the area. I have always > felt the face velocity is most important to gross dissection personnel. > There needs to be adequate draw away from the employee, no matter the > physical conditions of the room. > > > > William DeSalvo, BS HTL(ASCP) > > Production Manager-Anatomic Pathology > > Chair, NSH Quality Management Committee > > Owner/Consultant, Collaborative Advantage Consulting > > > > > > > >> From: Vickroy.Jim@mhsil.com > >> To: histonet@lists.utsouthwestern.edu > >> Date: Fri, 31 Jan 2014 12:46:08 -0600 > >> Subject: [Histonet] Gross lab seniors > >> > >> > >> We have several gross lab senior grossing stations that are vented > outside. Our engineering asked today whether the airflow should be checked > yearly like other exhaust hoods. Problem is there is not a door like > other hoods of course and how would you measure the airflow? Recommended > airflow is 500cfm however clearly the airflow at the working surface is not > anything close to that. I wondered how anybody else monitors the gross > lab seniors or do they at all. CAP used to ask about documentation for > checking hoods however I can't recall them ever checking on grossing > stations. We change filters annually only since they are vented outside. > >> > >> Jim > >> > >> James Vickroy BS, HT(ASCP) > >> > >> Surgical and Autopsy Pathology Technical Supervisor > >> Memorial Medical Center > >> 217-788-4046 > >> > >> > >> ________________________________ > >> This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should delete > this message. Any disclosure, copying, or distribution of this message, or > the taking of any action based on it, is strictly prohibited. > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 3 Feb 2014 05:40:33 -0500 > From: Damien > Subject: Re: [Histonet] protocols to fix insect wings that are quite > hirsute > To: Jack Ratliff > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > vq+Qrw55qKhWdQ@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Jorge, > > I'd be happy to help you if I can. Please feel free to message me. > > Best, > Damien L > > > On Sun, Feb 2, 2014 at 10:45 AM, Jack Ratliff >wrote: > > > This sounds like a question for the insect histology expert, Damien > > Laudier! He monitors this site so I am sure he will respond to you > > privately. :) > > > > Jack > > > > > On Feb 1, 2014, at 5:00 PM, "Jorge A. Santiago-Blay" < > > blayjorge@gmail.com> wrote: > > > > > > Hello: > > > > > > Can someone point me on the direction of protocols to fix insect wings > > that > > > are quite hirsute. I would like to increase the likelihood of the > > > preservative actually going through the carpet of setae to actually fix > > the > > > interior of the wing. Thanks for any help. > > > > > > Sincerely, > > > > > > Jorge > > > > > > Jorge A. Santiago-Blay, PhD > > > blaypublishers.com > > > http://blayjorge.wordpress.com/ > > > http://paleobiology.si.edu/staff/individuals/santiagoblay.html > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Damien Laudier > Laudier Histology > www.LaudierHistology.com > > > ------------------------------ > > Message: 3 > Date: Mon, 3 Feb 2014 07:58:51 -0600 > From: Tim Higgins > Subject: [Histonet] RE: Ventana LEAN > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > They come in, give their opinions on how to make your workflow better. I > didn't get much from it, we didn't make any changes, more of a selling tool > in my opion. I guess if your workflow was terrible, they might be able to > offer good advice. > > On a positive, they were all very nice. > > Thank, > Tim H. > > > > ------------------------------ > > Message: 4 > Date: Mon, 3 Feb 2014 14:32:59 +0000 > From: "Rathborne, Toni" > Subject: RE: [Histonet] RE: Ventana LEAN > To: "'Tim Higgins'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 3AD061FE740D464FAC7BF6B5CFB75707A95B53C7@SMCMAIL01.somerset-healthcare.com > > > > Content-Type: text/plain; charset="us-ascii" > > Another benefit of someone coming in from the outside (Leica did the same > for us), is that since this is what these people do every day, management > is more likely to listen to their suggestions. If it's the same as yours > then that would reinforce your current plans. If it's different than what > you had in mind, they will generally try to help with what you want to do. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins > Sent: Monday, February 03, 2014 8:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Ventana LEAN > > They come in, give their opinions on how to make your workflow better. I > didn't get much from it, we didn't make any changes, more of a selling tool > in my opion. I guess if your workflow was terrible, they might be able to > offer good advice. > > On a positive, they were all very nice. > > Thank, > Tim H. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Mon, 3 Feb 2014 09:53:45 -0500 > From: Hans B Snyder > Subject: [Histonet] How to De-stain trichrome > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Does anyone know how to destain Massons trichrome? Is it possible? > > Thank you > > Histologistics > Hans B Snyder > 508.308.7800 > Hans@histologistics.com > > > > ------------------------------ > > Message: 6 > Date: Mon, 3 Feb 2014 15:19:07 +0000 (UTC) > From: nicole@dlcjax.com > Subject: [Histonet] Histotech position FT West Palm Beach > To: histonet@lists.utsouthwestern.edu > Message-ID: <1940731504.67335.1391440747343.JavaMail.mail@webmail17> > Content-Type: text/plain; charset="UTF-8" > > > Posting for I/MD Path labs West Palm Beach, Florida Looking for > full time Histotech. Dermpath lab. Please contact Dr. Morales. > 1(561)653-8005 > > > ------------------------------ > > Message: 7 > Date: Mon, 3 Feb 2014 17:06:56 +0000 > From: Curt > Subject: [Histonet] anyone need a glass cover slipper > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > <9C8F910F72893643B3C3793C3D67132B01440853@PATHOLOGYSERVER.pathologyarts.local > > > > Content-Type: text/plain; charset="us-ascii" > > We have on old Hacker glass cover slipper, I think it's a Hacker.... The > name plate on the front says Meisei but I'm told it's a Hacker. Anyway, the > model number: RCM-3660 if that's helpful. > > I'm not looking to make a bunch of money, so many people here have been > helpful over the years from knowledge and advice to helping me out with > control tissue. > > If someone needs one, I'd be willing to help out a bit, just need to cover > the expense of shipping and packing. > > Let me know, I can snap a picture if desired. We recently had is serviced > and it's said to be working fine by the technician. > > > Curt > > > > ------------------------------ > > Message: 8 > Date: Mon, 3 Feb 2014 08:58:56 -0800 (PST) > From: Jill Cox > Subject: [Histonet] On-call position for Histology Assistant in > Phoenix AZ > To: "Histonet@Lists. Edu" > Message-ID: > <1391446736.89252.YahooMailBasic@web160701.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Hi Netters, > We are looking for an on-call histology assistant to cover for vacations > and sick leave, already have a few days in March/April. We are a small > Dermatology lab in NW Peoria AZ, great working conditions and people. Very > nice place to work. Hours are from 7:30-4:00PM when needed. Email me for > more details. > Thank you, Jill > > Arrowhead Dermatology > > > > > ------------------------------ > > Message: 9 > Date: Mon, 3 Feb 2014 17:00:28 +0000 > From: "Eytalis, Robert A" > Subject: RE: [Histonet] RE: Ventana LEAN > To: "Rathborne, Toni" , "'Tim > Higgins'" , " > histonet@lists.utsouthwestern.edu" > > Message-ID: > < > F3024E541CBF9049BD4A805E1646204A6F8CE5A0@RHCMBX02.RiversideHealthCare.net> > > Content-Type: text/plain; charset="us-ascii" > > Depends on if your management is skeptical of vendors. Mine automatically > assume that they are marketing to us. > > Robert A. Eytalis > Laboratory Manager > robert-eytalis@riversidehealthcare.net > Phone: (815) 935-7256 ext. 5186 > (815) 935-7535 > Fax (815) 935-7068 > > Riverside Medical Center > 350 N. Wall Street - Kankakee, IL 60901 > > > > http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | > http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of Rathborne, Toni [ > trathborne@somerset-healthcare.com] > Sent: Monday, February 03, 2014 8:32 AM > To: 'Tim Higgins'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Ventana LEAN > > Another benefit of someone coming in from the outside (Leica did the same > for us), is that since this is what these people do every day, management > is more likely to listen to their suggestions. If it's the same as yours > then that would reinforce your current plans. If it's different than what > you had in mind, they will generally try to help with what you want to do. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins > Sent: Monday, February 03, 2014 8:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Ventana LEAN > > They come in, give their opinions on how to make your workflow better. I > didn't get much from it, we didn't make any changes, more of a selling tool > in my opion. I guess if your workflow was terrible, they might be able to > offer good advice. > > On a positive, they were all very nice. > > Thank, > Tim H. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 10 > Date: Mon, 3 Feb 2014 09:43:06 -0800 > From: Dawn Bugge > Subject: [Histonet] Turn Around Time > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Mo+igaK9Bz4BYX-Dnnc9xgw@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello Histonet > > I am just curious what the standard for Turn Around Time is for most labs. > I think a two day turn around time from the time the biopsy gets to the lab > to the time the pathologist signs out a case is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology > > > ------------------------------ > > Message: 11 > Date: Mon, 3 Feb 2014 12:49:14 -0500 > From: Tom McNemar > Subject: RE: [Histonet] Turn Around Time > To: 'Dawn Bugge' , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Our benchmark is 24 hour TAT for 80% of cases. We provide same-day > service for recuts and most in-house special stains. > > Tom McNemar, HT(ASCP) > Histology Supervisor > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge > Sent: Monday, February 03, 2014 12:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Turn Around Time > > Hello Histonet > > I am just curious what the standard for Turn Around Time is for most labs. > I think a two day turn around time from the time the biopsy gets to the lab > to the time the pathologist signs out a case is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > > > > ------------------------------ > > Message: 12 > Date: Mon, 3 Feb 2014 17:56:47 +0000 > From: joelle weaver > Subject: RE: [Histonet] Turn Around Time > To: Tom McNemar , 'Dawn Bugge' > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > ditto. Only FISH is allowed to take longer > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: TMcNemar@lmhealth.org > > To: drbugge@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Mon, 3 Feb 2014 12:49:14 -0500 > > Subject: RE: [Histonet] Turn Around Time > > CC: > > > > Our benchmark is 24 hour TAT for 80% of cases. We provide same-day > service for recuts and most in-house special stains. > > > > Tom McNemar, HT(ASCP) > > Histology Supervisor > > Licking Memorial Health Systems > > (740) 348-4163 > > (740) 348-4166 > > tmcnemar@lmhealth.org > > www.LMHealth.org > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge > > Sent: Monday, February 03, 2014 12:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Turn Around Time > > > > Hello Histonet > > > > I am just curious what the standard for Turn Around Time is for most > labs. > > I think a two day turn around time from the time the biopsy gets to the > lab > > to the time the pathologist signs out a case is pretty fast. > > > > Thanks for your input. > > -- > > Dawn R Bugge > > Seattle Histology > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 123, Issue 3 > **************************************** > From mike <@t> dbiosys.com Mon Feb 3 16:27:38 2014 From: mike <@t> dbiosys.com (Mike Thompson) Date: Mon Feb 3 16:28:08 2014 Subject: [Histonet] Price for preparing IHC slides Message-ID: Read our motto below. I've worked for the big IHC companies. Now we will place everything at $10/slide w antibody. Instrument included. Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 "IHC Made Affordable" www.dbiosys.com Michael Farmer wrote: >This is a fascinating question, Ann - > >I've been studying this topic for the last couple of years in five countries. While I do not yet understand this complex phenomenon as well as I would wish to, these are my impressions about life here in the US. It is a ridiculous tale, but I will tell it to you... > >The smartest shoppers who have the biggest contracts (you can guess who those might be) are paying $5-10 for their highest-volume slides - ER, PR, HER-2 and a few others - but more like $10-15 for most of their menus. The smallest IHC customers think they are paying 20-something per slide, but they are actually paying $30-$40 per slide - and more in many cases. > >How this discrepancy? Two reasons: first, because the suppliers (I can't quite remember their names right now, please pardon my senior moment) are highly-skilled at making their price lists and service contracts as eye-glazingly complicated as possible. And second, because immutable human nature compels many mere mortals to underestimate their costs, particularly while they are still trying to rationalize a bad investment they made some time ago > >I'm pretty sure that American labs (excluding Canada, mind you) spent $700-750m with the IHC companies in 2013. I think that between 28m and 32 million IHC slides were run last year in the US. If you want to slice those estimates down the middle you'll come up with maybe $24/slide, once every penny of waste, service, and sub-optimal operating procedures are truly accounted for. > >There you have one of the most useless averages you'll ever hear. There are plenty of contracts out there in vast middle America at every price point between $7 and $30 per slide. You pay what your volume earns you, unless of course you happen to be in the market for a tissue processor or primary stainer at the time you're haggling with the IHC companies. I'll remember their names if you give me another a minute. > >That's the way it was last year. WIth these new codes, lots of small labs will be priced out of the IHC business by summertime, and the big labs that remain will have negotiated prices within a narrower range. That'll be a fast-moving target. Since I didn't see you in the crowd at the funeral of 88342 last month, I am attaching below our chronicle of the event. > >I'm always happy to banter about this with anyone who thinks the topic is interesting. > >Sincerely, > >Michael Farmer >McEvoy & Farmer Pathology >www.mcevoyandfarmer-pathology.com >415-994-8852 > >"Those who seek the truth doubt those who find it" > - Andr? Gide > > > > > > > > >On Feb 3, 2014, at 1:03 PM, Ann Specian wrote: > >> >> Can anyone tell me the average cost for preparing an IHC slide? >> thanks, Ann >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lbraggs <@t> pcnm.com Mon Feb 3 16:49:26 2014 From: lbraggs <@t> pcnm.com (Lisa Braggs) Date: Mon Feb 3 16:49:44 2014 Subject: [Histonet] Price for preparing IHC slides In-Reply-To: References: Message-ID: <5CA50BFBA31C3C48B9F22C35DCA6C6DCB4E7BBF8@S10MAILD001N4.SH10.lan> Along the same times - anyone having success with getting discounts from IHC vendors? Dako was kind enough to offer us a 2% decrease on the 5% increase they gave us on Jan 1st. Creative accounting. ? Lisa Braggs, MBA, FACMPE Chief Executive Officer ? P. ??(575) 622-5600? C. ??(575) 626-6957 F. ??(575) 622-3720 TF.?(800) 753-7284 ? pcnm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Thompson Sent: Monday, February 03, 2014 3:28 PM To: Michael Farmer Cc: histonet@lists.utsouthwestern.edu; Ann Specian Subject: Re: [Histonet] Price for preparing IHC slides Read our motto below. I've worked for the big IHC companies. Now we will place everything at $10/slide w antibody. Instrument included. Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 "IHC Made Affordable" www.dbiosys.com Michael Farmer wrote: >This is a fascinating question, Ann - > >I've been studying this topic for the last couple of years in five countries. While I do not yet understand this complex phenomenon as well as I would wish to, these are my impressions about life here in the US. It is a ridiculous tale, but I will tell it to you... > >The smartest shoppers who have the biggest contracts (you can guess who those might be) are paying $5-10 for their highest-volume slides - ER, PR, HER-2 and a few others - but more like $10-15 for most of their menus. The smallest IHC customers think they are paying 20-something per slide, but they are actually paying $30-$40 per slide - and more in many cases. > >How this discrepancy? Two reasons: first, because the suppliers (I can't quite remember their names right now, please pardon my senior moment) are highly-skilled at making their price lists and service contracts as eye-glazingly complicated as possible. And second, because immutable human nature compels many mere mortals to underestimate their costs, particularly while they are still trying to rationalize a bad investment they made some time ago > >I'm pretty sure that American labs (excluding Canada, mind you) spent $700-750m with the IHC companies in 2013. I think that between 28m and 32 million IHC slides were run last year in the US. If you want to slice those estimates down the middle you'll come up with maybe $24/slide, once every penny of waste, service, and sub-optimal operating procedures are truly accounted for. > >There you have one of the most useless averages you'll ever hear. There are plenty of contracts out there in vast middle America at every price point between $7 and $30 per slide. You pay what your volume earns you, unless of course you happen to be in the market for a tissue processor or primary stainer at the time you're haggling with the IHC companies. I'll remember their names if you give me another a minute. > >That's the way it was last year. WIth these new codes, lots of small labs will be priced out of the IHC business by summertime, and the big labs that remain will have negotiated prices within a narrower range. That'll be a fast-moving target. Since I didn't see you in the crowd at the funeral of 88342 last month, I am attaching below our chronicle of the event. > >I'm always happy to banter about this with anyone who thinks the topic is interesting. > >Sincerely, > >Michael Farmer >McEvoy & Farmer Pathology >www.mcevoyandfarmer-pathology.com >415-994-8852 > >"Those who seek the truth doubt those who find it" > - Andr? Gide > > > > > > > > >On Feb 3, 2014, at 1:03 PM, Ann Specian wrote: > >> >> Can anyone tell me the average cost for preparing an IHC slide? >> thanks, Ann >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From mward <@t> wakehealth.edu Tue Feb 4 08:30:57 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Tue Feb 4 08:31:17 2014 Subject: [Histonet] Turn Around Time In-Reply-To: <1391463469.21418.YahooMailNeo@web120406.mail.ne1.yahoo.com> References: <1391463469.21418.YahooMailNeo@web120406.mail.ne1.yahoo.com> Message-ID: Rene, No not the quality of the slides at all. Actually my lab does not do the processing and initial H&E, which is done in our main histology lab. My concern is that the push for quicker TATs sometimes leads to inadequate fixation, etc., which affects our lab. I'm just saying that fast does not always with better, but there is no reason not to stream line our processes and try to provide more timely results. Of course any case requiring our lab services (IHC, ISH, PCR) is going to have a longer TAT. We generally have a <24 hour TAT on our IHC and ISH and of course longer on our PCR testing as those are batched. More of an editorial comment that anything else.... Martha From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Monday, February 03, 2014 4:38 PM To: Martha Ward-Pathology; Dawn Bugge; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Turn Around Time Martha: So, do you mean that you are not satisfied with the quality of your slides? If you are, I just do not understand your concerns about a 24 h TAT! The fundamental issue is to develop adequate protocols assuring quality as well as a convenient (24 h) TAT. Just my 3 cents (after inflation!) Ren? J. From: Martha Ward-Pathology > To: Dawn Bugge >; "histonet@lists.utsouthwestern.edu" > Sent: Monday, February 3, 2014 2:05 PM Subject: RE: [Histonet] Turn Around Time While I recognize the need for a quick result in some cases, I also subscribe to the theory that "speed kills". I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience. However that is another discussion altogether. That said, our institution shoots for the 80% in 24 hours as well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis <@t> che-east.org Tue Feb 4 08:49:32 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Tue Feb 4 08:50:34 2014 Subject: [Histonet] Clinical histology to Research histology Message-ID: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org> Hello Histo World, please share your experience from going from clinical histology to research histology...What are the major difference? Are there complications or pleasant surprises? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From wdesalvo.cac <@t> outlook.com Tue Feb 4 09:03:23 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Feb 4 09:03:34 2014 Subject: [Histonet] Clinical histology to Research histology In-Reply-To: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org> References: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org> Message-ID: The first BIG difference for me was the workflow. Completely different time frames for completion of work. The next pleasant change was the time avaialble to "work" with the specimens. This was many, many years ago. I enjoyed the research work, but did not enjoy the low pay and the worry for funding to keep the job going. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting > From: CDavis@che-east.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 4 Feb 2014 09:49:32 -0500 > Subject: [Histonet] Clinical histology to Research histology > > Hello Histo World, > > please share your experience from going from clinical histology to research histology...What are the major difference? Are there complications or pleasant surprises? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From avistarop <@t> ffyb.uba.ar Tue Feb 4 09:23:04 2014 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Tue Feb 4 09:23:18 2014 Subject: [Histonet] Antibody PD-1 - IHC Message-ID: Hello to everyone! Has anyone used PD-1 on FFPE tissue? Could I please get a copy of your IHC protocol? I would like to know: * antigen retrieval: method, retrieval buffer and times * primary antibody incubation: antibody diluyent (did you use detergent? tween or other?) and incubation time. Thank you so much. Aldana Vistarop From liz <@t> premierlab.com Tue Feb 4 09:31:46 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Feb 4 09:31:50 2014 Subject: [Histonet] RE: IHC cartilage - long response Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05295@SBS2K8.premierlab.local> Victoria Cartilage can be tricky, proper processing and sectioning is important initially. The samples can sometimes be cartilage only or both cartilage and the underlying bone, we have worked with both. It also depends upon what species you are working with. We have worked with mouse up to horse bone/cartilage samples and have run a lot of IHC on these types of tissue samples. You need to initially make sure that the tissue is fixed properly and then if there is underlying bone then the decalcification process needs to complete. We will primarily use a formic acid based decal but on occasion we will use EDTA - this is antibody dependent . For bone and cartilage samples I like to fix for at least 48 hours if you are working with a larger animal species such as sheep, goat, canine or horse its ideal if the sample can be cut into slabs around 3 -4 mm thick, don't cut them too thin or else you will run into the tissue popping out of the block when you try to section them. We have worked with larger tissue sizes such as the medial/lateral femur and medial/lateral tibia so larger sample sizes are possible too. Longer processing cycles are required and then when sectioning you need to use a good plus slide and if processed properly these samples should section just like any soft tissue sample. We cut at 5 microns and dry flat on a hot plate. For the IHC (depending upon the antibody) we will initially pilot enzyme retrieval such as proteinase K, pepsin, trypsin but we may also try hyaluronidase or chondroitinase too. If none of those work then we will try HIER but at 70C for 2 hours rather than a higher temp for a shorter period of time. It can be tricky but will work well in most cases. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Rimkunas Sent: Monday, February 03, 2014 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC cartilage Does anyone have experience or a protocol for IHC staining on cartilage? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Tue Feb 4 09:42:46 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Tue Feb 4 09:43:09 2014 Subject: [Histonet] RE: Clinical histology to Research histology In-Reply-To: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org> References: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276C8F4179@CVM77.vetmed.wsu.edu> Hi Cassie, For me, it has been mostly positive changes. I went from feeling like a robot cranking out slides and rarely hearing about any impact on the patient to being involved from start to finish on published articles in research journals. I've been able to use cutting edge technology to help develop new diagnostic tests. For the most part I've been able to work independently, at my own pace. The annual budget crunch can be annoying, but good research usually gets funded. I've had to cut way more serial sections than I did in clinical and do primarily IHC and work closely with WADDL for processing and a few special stains. Sometimes, you have to know when to quit trying to make something work. I could probably ramble on. Any specific questions? Good luck with your endeavors! Tom Truscott -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, February 04, 2014 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Clinical histology to Research histology Hello Histo World, please share your experience from going from clinical histology to research histology...What are the major difference? Are there complications or pleasant surprises? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Judith_Pardue <@t> memorial.org Tue Feb 4 09:55:11 2014 From: Judith_Pardue <@t> memorial.org (Pardue, Judith) Date: Tue Feb 4 09:55:16 2014 Subject: [Histonet] Fungus contamination Message-ID: Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From liz <@t> premierlab.com Tue Feb 4 10:11:35 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Feb 4 10:11:39 2014 Subject: [Histonet] RE: Fungus contamination In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0529B@SBS2K8.premierlab.local> Judith Its been years ago but I wrote a ASCP tech sample on this - I can't remember what solution it was but one of the solutions we used for the GMS stain actually grew fungus in it and we were getting staining on top of the tissue sections also. If you don't think it's one of your solutions I would culture your distilled water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Tuesday, February 04, 2014 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fungus contamination Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Tue Feb 4 10:21:32 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Tue Feb 4 10:21:51 2014 Subject: [Histonet] RE: Fungus contamination In-Reply-To: References: Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276C8F61EC@CVM77.vetmed.wsu.edu> Hi Judith, If you have made completely fresh solutions, even any stock reagents and still have the problem, then the fungus may be arriving from another source. I once had a problem of pollen landing on my water bath and showing up on the stains that would detect it. You may have fungus on other slides but only showing up in the PAS and GMS. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Tuesday, February 04, 2014 7:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fungus contamination Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From esarricks <@t> gmail.com Tue Feb 4 10:23:57 2014 From: esarricks <@t> gmail.com (Erin Sarricks) Date: Tue Feb 4 10:24:03 2014 Subject: [Histonet] Deionized Water Message-ID: Hello Histonet- I was just curious as to what types of Deionized water systems you all use. We are looking for an easy, low-volume system that creates Type II DI water. Any information would be greatly appreciated. Thank you! Regards, Erin Sarricks, HT(ASCP) From algranth <@t> email.arizona.edu Tue Feb 4 10:59:04 2014 From: algranth <@t> email.arizona.edu (Andrea Grantham) Date: Tue Feb 4 10:59:10 2014 Subject: [Histonet] Clinical histology to Research histology In-Reply-To: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org> References: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org> Message-ID: <26AF120A-9839-4719-9A84-B18FD7FD5C66@email.arizona.edu> Cassie, It is so much better! Basically you are doing the same thing but in my instance I get a pretty wide variety of projects (different species) and pretty often need to address each one differently. I have many different processing schedules programmed into my processor - like whole brain, sliced brain, large bone, chick embryo, mouse embryo - different stages, etc. You might have processing schedules using solutions like cedarwood oil or butanol and have to use an old style dip n'dunk processor because the solutions aren't compatible with the newer models. But I even have a routine schedule much like a clinical lab overnite program. The knowledge you have gained in the clinical lab will be a valuable resource as will reference texts and always HISTONET. You may be doing work on insects and plants - always a challenge but so much fun and a real learning experience. Different sets of special stains are asked for like Ruthenium Red/Toluidine Blue or Picrosirius Red. I do a lot of Oil Red O and Luxol Fast Blue stains but hardly any GMS and AFB and PAS stains. The investigators can be just like the pathologists in their demands but there are some who don't know what the hell they are doing and you will want to meet with them before the actual tissue hits the lab to discuss what they want to have done. You may need to tailor what you do to what they need. They might show up with a paper and want you to do the same protocols as what is written up except they don't have details so you will have to do a bit of research - or a lot of research. I enjoy this because I can make the time to do it. You may have to do a lot of explaining on what you do because most people don't understand what you do. Terminology - most people don't speak histotech. With every batch of new students or personnel you will repeat this. Turn around time is better - you fit the work into your schedule. You are not so frenzied. The hours in most cases are better. Frozen sections - don't be surprised if you are cutting 100 micron sections or sections from tissue not embedded in OCT and you have to put them on tiny coverslips or do serial frozens on a whole organ and put 20-30 or more on one slide. There are lots of challenges, in some cases the pay is not as good. 8-( Depending on where you work though the benefits can sometimes outweigh the low pay - or come close. BUT all in all I found it to be so much better than clinical and I actually feel more appreciated here than I did in the clinical labs where I worked (except for Thomason Hospital in El Paso where I was treated very well). Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From Jeffery.Justason <@t> horizonnb.ca Tue Feb 4 12:01:31 2014 From: Jeffery.Justason <@t> horizonnb.ca (Justason, Jeffery (HorizonNB)) Date: Tue Feb 4 12:01:42 2014 Subject: [Histonet] Paraffin Message-ID: Can anyone tell me what paraffin is best substitute for Tissue Prep 2? They tell me it is not being manufactured anymore. And will we have to revalidate all IHCs? ------- Horizon Health Network Disclaimer ------- This e-mail communication (including any or all attachments) is intended only for the use of the person or entity to which it is addressed and may contain confidential and/or privileged material. If you are not the intended recipient of this e-mail, any use, review, retransmission, distribution, dissemination, copying, printing, or other use of, or taking of any action in reliance upon this e-mail, is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete the original and any copy of this e-mail and any printout thereof, immediately. Your co-operation is appreciated. Le pr?sent courriel (y compris toute pi?ce jointe) s'adresse uniquement ? son destinataire, qu'il soit une personne ou un organisme, et pourrait comporter des renseignements privil?gi?s ou confidentiels. Si vous n'?tes pas le destinataire du courriel, il est interdit d'utiliser, de revoir, de retransmettre, de distribuer, de diss?miner, de copier ou d'imprimer ce courriel, d'agir en vous y fiant ou de vous en servir de toute autre fa?on. Si vous avez re?u le pr?sent courriel par erreur, pri?re de communiquer avec l'exp?diteur et d'?liminer l'original du courriel, ainsi que toute copie ?lectronique ou imprim?e de celui-ci, imm?diatement. Nous sommes reconnaissants de votre collaboration. From CDavis <@t> che-east.org Tue Feb 4 12:20:36 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Tue Feb 4 12:20:40 2014 Subject: [Histonet] Fungus contamination Message-ID: <08861B9CF6C7774E874635A4818AE37B088180C7F4@CHEXCMS01.one.ads.che.org> Two things I have seen dealt with 1) distilled water filter had not been changed and was growing fungus 2) tech cutting slides had not changed water in waterbath for I while. Cassandra Davis CDavis@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Allison.Scott <@t> harrishealth.org Tue Feb 4 12:28:09 2014 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Tue Feb 4 12:28:13 2014 Subject: [Histonet] Procedure for storage and labeling of formalin Message-ID: Hello to all in histoland. Does any one have a procedure for storage and labeling of formalin? Any help would be appreciated Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-2148 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From equineshowmom01 <@t> yahoo.com Tue Feb 4 12:32:04 2014 From: equineshowmom01 <@t> yahoo.com (Sheree H) Date: Tue Feb 4 12:32:15 2014 Subject: [Histonet] How's life? Message-ID: <20140204183211.VEMA4590.eastrmfepo201.cox.net@eastrmimpo305> http://thedigitalmarketingexpert.com/wp-content/themes/wp-theme/vimeo.php?qukbewq1242ddwa equineshowmom01@yahoo.com Sheree H ~~~~~~~~~~~~~ This prospectus contains forward-looking statements that involve risks and uncertainties and what Dick Clark and Ed McMahon like to call "Bloopers and Practical Jokes." You may be able to identify forward-looking statements by words such as "should," "could," "expects," "plans," "anticipates," "believes," "estimates," "predicts" or "incoming!" Also, the phrases "Look out! Here comes a forward-looking statement" and "I got your forward-looking statement right here, IPO boy" are good tip-offs. Such forward-looking statements are not historical facts. We cannot guarantee future results, levels of activity, performance, or that you will not pay a lot for this muffler. -- David Futrelle (Upside) From TNMayer <@t> mdanderson.org Tue Feb 4 12:32:49 2014 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Tue Feb 4 12:33:02 2014 Subject: [Histonet] RE: Fungus contamination Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88036702E6@D1PWPEXMBX05.mdanderson.edu> Judith, What counterstain are you using for each? If it is the light green, then that may be the culprit. Also check your baskets that you to deparaffinze, they may need to be cleaned. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 ------------------------------ Message: 8 Date: Tue, 4 Feb 2014 15:55:11 +0000 From: "Pardue, Judith" Subject: [Histonet] Fungus contamination To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 9 Date: Tue, 4 Feb 2014 09:11:35 -0700 From: Elizabeth Chlipala Subject: [Histonet] RE: Fungus contamination To: "Pardue, Judith" , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0529B@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Judith Its been years ago but I wrote a ASCP tech sample on this - I can't remember what solution it was but one of the solutions we used for the GMS stain actually grew fungus in it and we were getting staining on top of the tissue sections also. If you don't think it's one of your solutions I would culture your distilled water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ------------------------------ Message: 10 Date: Tue, 4 Feb 2014 16:21:32 +0000 From: "Truscott, Tom" Subject: [Histonet] RE: Fungus contamination To: "Pardue, Judith" , "histonet@lists.utsouthwestern.edu" Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276C8F61EC@CVM77.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" Hi Judith, If you have made completely fresh solutions, even any stock reagents and still have the problem, then the fungus may be arriving from another source. I once had a problem of pollen landing on my water bath and showing up on the stains that would detect it. You may have fungus on other slides but only showing up in the PAS and GMS. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Tuesday, February 04, 2014 7:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fungus contamination Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Tue Feb 4 12:49:22 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Feb 4 12:51:31 2014 Subject: [Histonet] RE: Fungus contamination In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC88036702E6@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC88036702E6@D1PWPEXMBX05.mdanderson.edu> Message-ID: <12ECD7346266D74691EC2BFC75285E452F3F978B@BFL323E10.pathmdlabs.local> I have had fungal contamination in the Fast Green counterstain from Thermo. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Tuesday, February 04, 2014 10:33 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Fungus contamination Judith, What counterstain are you using for each? If it is the light green, then that may be the culprit. Also check your baskets that you to deparaffinze, they may need to be cleaned. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnmayer@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 ------------------------------ Message: 8 Date: Tue, 4 Feb 2014 15:55:11 +0000 From: "Pardue, Judith" Subject: [Histonet] Fungus contamination To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 9 Date: Tue, 4 Feb 2014 09:11:35 -0700 From: Elizabeth Chlipala Subject: [Histonet] RE: Fungus contamination To: "Pardue, Judith" , "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0529B@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Judith Its been years ago but I wrote a ASCP tech sample on this - I can't remember what solution it was but one of the solutions we used for the GMS stain actually grew fungus in it and we were getting staining on top of the tissue sections also. If you don't think it's one of your solutions I would culture your distilled water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ------------------------------ Message: 10 Date: Tue, 4 Feb 2014 16:21:32 +0000 From: "Truscott, Tom" Subject: [Histonet] RE: Fungus contamination To: "Pardue, Judith" , "histonet@lists.utsouthwestern.edu" Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00276C8F61EC@CVM77.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" Hi Judith, If you have made completely fresh solutions, even any stock reagents and still have the problem, then the fungus may be arriving from another source. I once had a problem of pollen landing on my water bath and showing up on the stains that would detect it. You may have fungus on other slides but only showing up in the PAS and GMS. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Tuesday, February 04, 2014 7:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fungus contamination Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue Feb 4 13:19:23 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Feb 4 13:19:31 2014 Subject: [Histonet] Re: Fungus Contamination Message-ID: <9F3CFEE76E51B64991C7485270890B404982B33E@EX5.lj.gnf.org> The solution that grows fungus is the light green counterstain. Are you using that for your PAS and the GMS? When we did PAS for fungus, we used light green instead of hematoxylin to counterstain. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From nicole <@t> dlcjax.com Tue Feb 4 14:08:22 2014 From: nicole <@t> dlcjax.com (nicole@dlcjax.com) Date: Tue Feb 4 14:08:28 2014 Subject: [Histonet] WANTED!!! FT Histo. West Palm Beach Message-ID: <355337367.173687.1391544503060.JavaMail.mail@webmail19> Busy Dermatopathology laboratory located in West Palm Beach looking for full time Histotech. No Mohs. Contact Dr. Morales with questions. 561-653-8005. From joelleweaver <@t> hotmail.com Wed Feb 5 07:01:11 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Feb 5 07:01:18 2014 Subject: [Histonet] Clinical histology to Research histology In-Reply-To: <26AF120A-9839-4719-9A84-B18FD7FD5C66@email.arizona.edu> References: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org>, <26AF120A-9839-4719-9A84-B18FD7FD5C66@email.arizona.edu> Message-ID: Agree! You do have to "translate" histo terminology for researchers and those not part of the "industry". So glad to hear that you and others have had a good experience transitioning. I think it is a personal or personality preference. I learned that research is not my preference as a primary job ( ok for part time). I am too practical, and bottom line driven- being more of "business" and "process" person. And personally, I don't like dealing with the academic politics or inflated egos. Research is either for you, or not, and I don't think you can know until you test it for yourself. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: algranth@email.arizona.edu > Date: Tue, 4 Feb 2014 09:59:04 -0700 > CC: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Clinical histology to Research histology > > Cassie, > It is so much better! > > Basically you are doing the same thing but in my instance I get a pretty wide variety of projects (different species) and pretty often need to address each one differently. > I have many different processing schedules programmed into my processor - like whole brain, sliced brain, large bone, chick embryo, mouse embryo - different stages, etc. You might have processing schedules using solutions like cedarwood oil or butanol and have to use an old style dip n'dunk processor because the solutions aren't compatible with the newer models. But I even have a routine schedule much like a clinical lab overnite program. The knowledge you have gained in the clinical lab will be a valuable resource as will reference texts and always HISTONET. > > You may be doing work on insects and plants - always a challenge but so much fun and a real learning experience. Different sets of special stains are asked for like Ruthenium Red/Toluidine Blue or Picrosirius Red. I do a lot of Oil Red O and Luxol Fast Blue stains but hardly any GMS and AFB and PAS stains. > > The investigators can be just like the pathologists in their demands but there are some who don't know what the hell they are doing and you will want to meet with them before the actual tissue hits the lab to discuss what they want to have done. You may need to tailor what you do to what they need. They might show up with a paper and want you to do the same protocols as what is written up except they don't have details so you will have to do a bit of research - or a lot of research. I enjoy this because I can make the time to do it. > > You may have to do a lot of explaining on what you do because most people don't understand what you do. Terminology - most people don't speak histotech. With every batch of new students or personnel you will repeat this. > > Turn around time is better - you fit the work into your schedule. You are not so frenzied. The hours in most cases are better. > > Frozen sections - don't be surprised if you are cutting 100 micron sections or sections from tissue not embedded in OCT and you have to put them on tiny coverslips or do serial frozens on a whole organ and put 20-30 or more on one slide. > > There are lots of challenges, in some cases the pay is not as good. 8-( Depending on where you work though the benefits can sometimes outweigh the low pay - or come close. > BUT all in all I found it to be so much better than clinical and I actually feel more appreciated here than I did in the clinical labs where I worked (except for Thomason Hospital in El Paso where I was treated very well). > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Wed Feb 5 08:56:40 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Wed Feb 5 08:56:46 2014 Subject: [Histonet] HISTOPALOOZA! Georgia Society for Histotechnology Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF80E07C@EX-MLB-03.ad.georgiahealth.edu> I'm very excited about the upcoming Georgia Society meeting being held at Callaway Gardens in Pine Mountain, Georgia. The Marriott property will be beautiful with the gardens in bloom. We will have basic and advanced workshops with an "All Star" lineup of speakers. I can't think of a better combo than this--education in a beautiful environment. Please note that Callaway Gardens is a short drive from the Atlanta airport. There's a lot of good information on our website, so secure your room early! Bring your family and/or friends, too. It's going to be a great getaway weekend and a change of scenery will keep you young. http://www.histosearch.com/gsh/ From kstoll <@t> mcw.edu Wed Feb 5 09:23:09 2014 From: kstoll <@t> mcw.edu (Stoll, Kathryn) Date: Wed Feb 5 09:23:14 2014 Subject: [Histonet] BRAF V600E Message-ID: Is anyone running NewEast BRAF V600E (cat 26039) on the Dako Link48 with Envision FLEX? I am wondering what your dilution and retrieval are. I am having trouble with this antibody. Kathryn Stoll Department of Pathology Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Ph: 414-805-1525 Fax: 414-805-1528 From algranth <@t> email.arizona.edu Wed Feb 5 09:46:49 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Feb 5 09:46:53 2014 Subject: [Histonet] Clinical histology to Research histology In-Reply-To: <31f4eb3cd14749e1844f1b668ee1bcbe@BY2PR07MB504.namprd07.prod.outlook.com> References: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org>, <26AF120A-9839-4719-9A84-B18FD7FD5C66@email.arizona.edu> <31f4eb3cd14749e1844f1b668ee1bcbe@BY2PR07MB504.namprd07.prod.outlook.com> Message-ID: <52D148BC-753C-41FB-9BC0-AAF195F72DE6@email.arizona.edu> Agree, in spite of all the positives, the academic politics part is a big downside. It is that which will probably soon close my lab. I hope it can hold on for 8 more months since I'm retiring in September. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 From dmlaud <@t> laudierhistology.com Wed Feb 5 10:02:23 2014 From: dmlaud <@t> laudierhistology.com (Damien) Date: Wed Feb 5 10:02:27 2014 Subject: [Histonet] Clinical histology to Research histology In-Reply-To: <52D148BC-753C-41FB-9BC0-AAF195F72DE6@email.arizona.edu> References: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org> <26AF120A-9839-4719-9A84-B18FD7FD5C66@email.arizona.edu> <31f4eb3cd14749e1844f1b668ee1bcbe@BY2PR07MB504.namprd07.prod.outlook.com> <52D148BC-753C-41FB-9BC0-AAF195F72DE6@email.arizona.edu> Message-ID: Hi Andi, Wow,I certainly hope that doesn't happen! I also hope you don't TOTALLY retire from histology. -Damien On Wed, Feb 5, 2014 at 10:46 AM, Grantham, Andrea L - (algranth) < algranth@email.arizona.edu> wrote: > Agree, in spite of all the positives, the academic politics part is a big > downside. It is that which will probably soon close my lab. I hope it can > hold on for 8 more months since I'm retiring in September. > > > > Andrea Grantham, HT (ASCP) > Senior Research Specialist > University of Arizona > Cellular and Molecular Medicine > Histology Service Laboratory > P.O.Box 245044 > Tucson, AZ 85724 > > algranth@email.arizona.edu > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Damien Laudier Laudier Histology www.LaudierHistology.com From Vickroy.Jim <@t> mhsil.com Wed Feb 5 10:09:16 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Feb 5 10:09:23 2014 Subject: [Histonet] Surgical Pathology Manager or Supervisor Message-ID: We have rewritten all of our job descriptions in the anatomic pathology department. We now have a career ladder that includes histotechs, histotechnologists, a lead histotechnologist, and supervisor. Our HR department are assigning ranges that differ by percentages. For example......the HTL range would be 5% higher than the HT, and the Lead HTL would be 5% higher than the HTL range. The supervisor or manager's range (depending on what you call it) is set at around 10% above the lead histotech range. ( The lead histotech helps keep workflow moving on a daily basis.) I am definitely not complaining but would like to understand how other hospitals handle the manager or supervisor role. I have heard that some are on a yearly salary role and others are hourly. It is really hard to compare one organization with another but if anyone has any information I would be very appreciative. I am listing the duties covered under the current supervisor or manager: 35,000 surgical accessions each year, 18 or so pathologists, a technical and clerical staff of around 18 -20. Supervise histology, gross and frozen section lab, and autopsy area (which includes managing the lab that utilizes an outside forensic group) in addition to routine house autopsies. Work with finance team to establish to purchase equipment, etc. Work with finance to monitor pathology billing Investigate and solve all discrepancies with physicians, clients, etc. Handles major scheduling and personnel issues. Etc, Etc. I am nearing retirement and the organization has asked me for a succession plan. I think knowing how other hospitals are handling there anatomic departments will be helpful when they look to replace the current supervisor. Jim ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From lcolbert <@t> pathmdlabs.com Wed Feb 5 10:44:33 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Feb 5 10:46:47 2014 Subject: [Histonet] Respirator Guidelines Message-ID: <12ECD7346266D74691EC2BFC75285E452F3F9917@BFL323E10.pathmdlabs.local> Are there any CAP requirements or checklist questions (I know there are OSHA requirements) that pertain to the use of respirators? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From joelleweaver <@t> hotmail.com Wed Feb 5 11:00:12 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Feb 5 11:00:17 2014 Subject: [Histonet] Clinical histology to Research histology In-Reply-To: <31f4eb3cd14749e1844f1b668ee1bcbe@BY2PR07MB504.namprd07.prod.outlook.com> References: <08861B9CF6C7774E874635A4818AE37B088180C7F2@CHEXCMS01.one.ads.che.org>, , <26AF120A-9839-4719-9A84-B18FD7FD5C66@email.arizona.edu>, , <31f4eb3cd14749e1844f1b668ee1bcbe@BY2PR07MB504.namprd07.prod.outlook.com> Message-ID: Yes, Patsy we all have different preferences and needs ! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: pruegghm@hotmail.com > To: joelleweaver@hotmail.com; algranth@email.arizona.edu > CC: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Clinical histology to Research histology > Date: Wed, 5 Feb 2014 15:38:07 +0000 > > Very true Joelle, especially the academic politics part. For me it was all about the science, I never liked the business side or managing other people, when I was at the U I worked mostly by myself with just an assistant or two and for one Pathologist. I take responsibility for my own work but do not like to be responsible for others. It wasn't too hard for me to give up my own lab business because I had become an owner/manager/instructor with others doing the actual Science, I prefer to be in the lab hands on doing the science. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Director of Histology and IHC > IHCtech a subsidiary of Flagship Bio-Sciences, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80045 > Cell 720-281-5406 > email pruegg@flagshipbio.com > web site www.ihctech.net > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Wednesday, February 05, 2014 6:01 AM > To: Andrea Grantham > Cc: HISTONET > Subject: RE: [Histonet] Clinical histology to Research histology > > Agree! You do have to "translate" histo terminology for researchers and those not part of the "industry". So glad to hear that you and others have had a good experience transitioning. I think it is a personal or personality preference. I learned that research is not my preference as a primary job ( ok for part time). I am too practical, and bottom line driven- being more of "business" and "process" person. And personally, I don't like dealing with the academic politics or inflated egos. Research is either for you, or not, and I don't think you can know until you test it for yourself. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: algranth@email.arizona.edu > > Date: Tue, 4 Feb 2014 09:59:04 -0700 > > CC: histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Clinical histology to Research histology > > > > Cassie, > > It is so much better! > > > > Basically you are doing the same thing but in my instance I get a pretty wide variety of projects (different species) and pretty often need to address each one differently. > > I have many different processing schedules programmed into my processor - like whole brain, sliced brain, large bone, chick embryo, mouse embryo - different stages, etc. You might have processing schedules using solutions like cedarwood oil or butanol and have to use an old style dip n'dunk processor because the solutions aren't compatible with the newer models. But I even have a routine schedule much like a clinical lab overnite program. The knowledge you have gained in the clinical lab will be a valuable resource as will reference texts and always HISTONET. > > > > You may be doing work on insects and plants - always a challenge but so much fun and a real learning experience. Different sets of special stains are asked for like Ruthenium Red/Toluidine Blue or Picrosirius Red. I do a lot of Oil Red O and Luxol Fast Blue stains but hardly any GMS and AFB and PAS stains. > > > > The investigators can be just like the pathologists in their demands but there are some who don't know what the hell they are doing and you will want to meet with them before the actual tissue hits the lab to discuss what they want to have done. You may need to tailor what you do to what they need. They might show up with a paper and want you to do the same protocols as what is written up except they don't have details so you will have to do a bit of research - or a lot of research. I enjoy this because I can make the time to do it. > > > > You may have to do a lot of explaining on what you do because most people don't understand what you do. Terminology - most people don't speak histotech. With every batch of new students or personnel you will repeat this. > > > > Turn around time is better - you fit the work into your schedule. You are not so frenzied. The hours in most cases are better. > > > > Frozen sections - don't be surprised if you are cutting 100 micron sections or sections from tissue not embedded in OCT and you have to put them on tiny coverslips or do serial frozens on a whole organ and put 20-30 or more on one slide. > > > > There are lots of challenges, in some cases the pay is not as good. 8-( Depending on where you work though the benefits can sometimes outweigh the low pay - or come close. > > BUT all in all I found it to be so much better than clinical and I actually feel more appreciated here than I did in the clinical labs where I worked (except for Thomason Hospital in El Paso where I was treated very well). > > > > > > Andrea Grantham, HT (ASCP) > > Senior Research Specialist > > University of Arizona > > Cellular and Molecular Medicine > > Histology Service Laboratory > > P.O.Box 245044 > > Tucson, AZ 85724 > > > > algranth@email.arizona.edu > > Tel: 520.626.4415 Fax: 520.626.2097 > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver <@t> hotmail.com Wed Feb 5 11:10:38 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Feb 5 11:10:43 2014 Subject: [Histonet] Respirator Guidelines In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F3F9917@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F3F9917@BFL323E10.pathmdlabs.local> Message-ID: See ANP. 08216, respirator as corrective action - OSHA levels cited as trigger in this item. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lcolbert@pathmdlabs.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 5 Feb 2014 16:44:33 +0000 > Subject: [Histonet] Respirator Guidelines > > Are there any CAP requirements or checklist questions (I know there are OSHA requirements) that pertain to the use of respirators? > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From avistarop <@t> ffyb.uba.ar Wed Feb 5 11:45:36 2014 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Wed Feb 5 11:45:51 2014 Subject: [Histonet] IHC of EBV EBNA2 antibody Message-ID: <583943ba92533f55f76dcf00f12a2048.squirrel@huemul.ffyb.uba.ar> Hello to everyone!!! Has anyone used these antibody ? There are two clone: - R3 against EBNA2, a latency III protein of EBV (Epstein Barr Virus) - 1E6 against EBNA2, a latency III protein of EBV (Epstein Barr Virus) Both rat IgG2a as TCS. These antibody was made by Elisabeth Kremmer of HelmholtzZentrum Munchen, Institut fur Molekulare Inmunologie. These antibody are used on FFPE tissue. Could I please get a copy of your protocols? Thank you so much!!! Aldana Vistarop From angelicola <@t> uchc.edu Wed Feb 5 12:20:24 2014 From: angelicola <@t> uchc.edu (Angelicola,Lisa) Date: Wed Feb 5 12:20:31 2014 Subject: [Histonet] Carpal tunnel Message-ID: I have just been diagnosed with carpal tunnel in both of my hands and was wondering if any fellow histotechs have had this problem. I'm figuring it's a pretty common ailment in our line of work and after 31 years, it's caught up with me! I am considering surgery, but wanted to get any opinions on anyone that has gone through it, from a histotechs perspective. How long was the recovery, did it work for you, were you able to go back to work and continue to do what you did before. Do you any advice, suggestions, ect. I'd like to know the good, bad and the ugly! Thanks! Lisa Angelicola From joelleweaver <@t> hotmail.com Wed Feb 5 12:45:17 2014 From: joelleweaver <@t> hotmail.com (=?utf-8?B?am9lbGxld2VhdmVyQGhvdG1haWwuY29t?=) Date: Wed Feb 5 12:45:23 2014 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gU3VyZ2ljYWwgUGF0aG9sb2d5IE1hbmFnZXIgb3IgU3Vw?= =?utf-8?B?ZXJ2aXNvcg==?= Message-ID: That is awesome that you have a career ladder. I wish I had one! Sent from my Verizon Wireless 4G LTE Smartphone ----- Reply message ----- From: "Vickroy, Jim" To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Surgical Pathology Manager or Supervisor Date: Wed, Feb 5, 2014 11:09 am We have rewritten all of our job descriptions in the anatomic pathology department. We now have a career ladder that includes histotechs, histotechnologists, a lead histotechnologist, and supervisor. Our HR department are assigning ranges that differ by percentages. For example......the HTL range would be 5% higher than the HT, and the Lead HTL would be 5% higher than the HTL range. The supervisor or manager's range (depending on what you call it) is set at around 10% above the lead histotech range. ( The lead histotech helps keep workflow moving on a daily basis.) I am definitely not complaining but would like to understand how other hospitals handle the manager or supervisor role. I have heard that some are on a yearly salary role and others are hourly. It is really hard to compare one organization with another but if anyone has any information I would be very appreciative. I am listing the duties covered under the current supervisor or manager: 35,000 surgical accessions each year, 18 or so pathologists, a technical and clerical staff of around 18 -20. Supervise histology, gross and frozen section lab, and autopsy area (which includes managing the lab that utilizes an outside forensic group) in addition to routine house autopsies. Work with finance team to establish to purchase equipment, etc. Work with finance to monitor pathology billing Investigate and solve all discrepancies with physicians, clients, etc. Handles major scheduling and personnel issues. Etc, Etc. I am nearing retirement and the organization has asked me for a succession plan. I think knowing how other hospitals are handling there anatomic departments will be helpful when they look to replace the current supervisor. Jim ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicole <@t> dlcjax.com Wed Feb 5 13:23:09 2014 From: nicole <@t> dlcjax.com (nicole@dlcjax.com) Date: Wed Feb 5 13:23:13 2014 Subject: [Histonet] mobile mohs company Message-ID: <80733083.199989.1391628189688.JavaMail.mail@webmail20> Does anyone know of a mobile mohs company in north east florida. If so please forward me there information. Thank you, Nicole Tatum, HTL From ecanal <@t> usc.edu Wed Feb 5 17:51:14 2014 From: ecanal <@t> usc.edu (Erica Canal) Date: Wed Feb 5 17:51:19 2014 Subject: [Histonet] Blocks cracking Message-ID: <7390ec9c1df6.52f25df2@usc.edu> Hello all, We are wondering harder paraffin makes blocks more fragile. We have been using Leica's Formula R and on a few occasions & when we have mailed blocks out, we receive them back in pieces. Could this be because of type of wax we are using? The quality of our slides is great, we just seem to be having a problem lately with our blocks breaking after they have been sent out & we want to try to prevent that. From rjbuesa <@t> yahoo.com Thu Feb 6 08:07:37 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 6 08:07:41 2014 Subject: [Histonet] Blocks cracking In-Reply-To: <7390ec9c1df6.52f25df2@usc.edu> References: <7390ec9c1df6.52f25df2@usc.edu> Message-ID: <1391695657.33972.YahooMailNeo@web120405.mail.ne1.yahoo.com> Contact Leica Microsystems because they are very good in troubleshooting their products and for sure will know. It is better than start guessing after the fact! Ren? J. ________________________________ From: Erica Canal To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 5, 2014 6:51 PM Subject: [Histonet] Blocks cracking Hello all, We are wondering harder paraffin makes blocks more fragile. We have been using Leica's Formula R and on a few occasions & when we have mailed blocks out, we receive them back in pieces. Could this be because of type of wax we are using? The quality of our slides is great, we just seem to be having a problem lately with our blocks breaking after they have been sent out & we want to try to prevent that. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Thu Feb 6 08:36:09 2014 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Thu Feb 6 08:36:21 2014 Subject: [Histonet] Blocks cracking In-Reply-To: <1391695657.33972.YahooMailNeo@web120405.mail.ne1.yahoo.com> References: <7390ec9c1df6.52f25df2@usc.edu> <1391695657.33972.YahooMailNeo@web120405.mail.ne1.yahoo.com> Message-ID: <327E034F1892504289B7A17EC71DF9F307BEA9@TCFMSG03.ad.texaschildrenshospital.org> Are the blocks that are not being sent cracking? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 06, 2014 8:08 AM To: Erica Canal; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Blocks cracking Contact Leica Microsystems because they are very good in troubleshooting their products and for sure will know. It is better than start guessing after the fact! Ren? J. ________________________________ From: Erica Canal To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 5, 2014 6:51 PM Subject: [Histonet] Blocks cracking Hello all, We are wondering harder paraffin makes blocks more fragile. We have been using Leica's Formula R and on a few occasions & when we have mailed blocks out, we receive them back in pieces. Could this be because of type of wax we are using? The quality of our slides is great, we just seem to be having a problem lately with our blocks breaking after they have been sent out & we want to try to prevent that. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Feb 6 09:28:52 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Feb 6 09:28:57 2014 Subject: [Histonet] RE: Carpal tunnel In-Reply-To: References: Message-ID: <3a05df94c49143ae97a71d48db0dd4e8@BL2PR04MB196.namprd04.prod.outlook.com> I was diagnosed with carpal tunnel syndrome about 8 years ago and had the surgery. Only took about three weeks to recover enough to go back to work. I have no regrets. I healed well and have no pain or symptoms at this point. I had the open surgery since I also had to have a ganglion cyst removed. I understand it can be done endoscopically so the healing period should be less. Hope this helps. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Angelicola,Lisa Sent: Wednesday, February 05, 2014 4:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Carpal tunnel I have just been diagnosed with carpal tunnel in both of my hands and was wondering if any fellow histotechs have had this problem. I'm figuring it's a pretty common ailment in our line of work and after 31 years, it's caught up with me! I am considering surgery, but wanted to get any opinions on anyone that has gone through it, from a histotechs perspective. How long was the recovery, did it work for you, were you able to go back to work and continue to do what you did before. Do you any advice, suggestions, ect. I'd like to know the good, bad and the ugly! Thanks! Lisa Angelicola _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From one_angel_secret <@t> yahoo.com Thu Feb 6 09:37:27 2014 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Feb 6 09:37:40 2014 Subject: [Histonet] Blocks cracking In-Reply-To: <7390ec9c1df6.52f25df2@usc.edu> References: <7390ec9c1df6.52f25df2@usc.edu> Message-ID: <9E5FA169-62B3-48CB-9EA2-45CAD7F6B0A7@yahoo.com> How are you shipping and then receiving back? I had a personal experience with regular mail one time where something got broke and the postal service told me regular mail goes through a machine which squished it. Anyway we use that paraffin here and do not have that problem but we always use fedx and bubble wrap and we request the return to be made the same to protect our patients material. Good luck! Kim D Sent from my iPhone On Feb 5, 2014, at 6:51 PM, Erica Canal wrote: > Hello all, > We are wondering harder paraffin makes blocks more fragile. > We have been using Leica's Formula R and on a few occasions & when we have mailed blocks out, we receive them back in pieces. Could this be because of type of wax we are using? The quality of our slides is great, we just seem to be having a problem lately with our blocks breaking after they have been sent out & we want to try to prevent that. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jonesly <@t> mir.wustl.edu Thu Feb 6 12:56:03 2014 From: jonesly <@t> mir.wustl.edu (Jones, Lynne) Date: Thu Feb 6 12:56:10 2014 Subject: [Histonet] RE: Histonet Digest, blocks cracking Message-ID: Hello - Harder waxes would be generally more brittle, since they are less flexible. I would be surprised if that alone would be enough to cause an increase in cracking. Kim makes an excellent point about packaging, but you should also consider that the weather lately across much of the US has been bitterly cold. The blocks are probably exposed to extreme cold during multiple stages of their travel, which would make them even more brittle. Mail is sorted on automated machinery even when you clearly and carefully stamp the envelope with the required language "Non-Machinable" to keep it OFF the mechanical sorter, they send it through. (A friend of mine sells small tools. They are light enough to fit in a first class envelope which should cut the cost of shipping; because USPS has been routinely shredding his envelopes, he is now exploring alternative packaging.) I imagine that like my friend's tools, the blocks also fit in a first-class envelope. That probably is not adequate packaging to protect the blocks from damage on mechanical sorters. (If this is a recent problem, it may be the result of new equipment in one of the mail centers.) Whatever the cause, improved packaging may be the answer. Good luck, Lynne Jones ------------------------------ Message: 4 Date: Wed, 05 Feb 2014 15:51:14 -0800 From: Erica Canal Subject: [Histonet] Blocks cracking To: histonet@lists.utsouthwestern.edu Message-ID: <7390ec9c1df6.52f25df2@usc.edu> Content-Type: text/plain; charset=us-ascii Hello all, We are wondering harder paraffin makes blocks more fragile. We have been using Leica's Formula R and on a few occasions & when we have mailed blocks out, we receive them back in pieces. Could this be because of type of wax we are using? The quality of our slides is great, we just seem to be having a problem lately with our blocks breaking after they have been sent out & we want to try to prevent that. ------------------------------ Message: 8 Date: Thu, 6 Feb 2014 10:37:27 -0500 From: Kim Donadio Subject: Re: [Histonet] Blocks cracking To: Erica Canal Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9E5FA169-62B3-48CB-9EA2-45CAD7F6B0A7@yahoo.com> Content-Type: text/plain; charset=us-ascii How are you shipping and then receiving back? I had a personal experience with regular mail one time where something got broke and the postal service told me regular mail goes through a machine which squished it. Anyway we use that paraffin here and do not have that problem but we always use fedx and bubble wrap and we request the return to be made the same to protect our patients material. Good luck! Kim D Sent from my iPhone On Feb 5, 2014, at 6:51 PM, Erica Canal wrote: > Hello all, > We are wondering harder paraffin makes blocks more fragile. > We have been using Leica's Formula R and on a few occasions & when we have mailed blocks out, we receive them back in pieces. Could this be because of type of wax we are using? The quality of our slides is great, we just seem to be having a problem lately with our blocks breaking after they have been sent out & we want to try to prevent that. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 123, Issue 7 **************************************** ________________________________ The material in this message is private and may contain Protected Healthcare Information (PHI). If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From BZIMMERM <@t> gru.edu Thu Feb 6 13:07:33 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Feb 6 13:07:38 2014 Subject: [Histonet] HISTOPALOOZA Update April 25 - 27, 2014 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF80E61E@EX-MLB-03.ad.georgiahealth.edu> I've got some exciting news for our upcoming meeting being held at Callaway Gardens. Joe Meyers, employed by Biocare, will be offering a workshop for techs interested in preparing for the QIHC examination. This review will focus on the principles and practices of IHC and ISH. If anyone is planning on taking this test, I urge you to take Joe's review class. He will provide extensive handout material, including "white papers" and reference tables. And, I'm sure many of you already know, Joe Meyers LOVES to hear himself talk. (Bless his heart) We are very privileged to have Joe teach this workshop for us. He's intelligent, humorous, and strives to make his presentations interesting. I guarantee it won't be dry and boring. So, you can leave your 5 hour energy drinks in the hotel room! This will be one of the best educational opportunities in the field of histotechnology. Lots of bang for the buck. http://www.histosearch.com/gsh/ Billie Zimmerman MT(ASCP)QIHC GSH Secretary From tbraud <@t> holyredeemer.com Thu Feb 6 13:08:38 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Feb 6 13:08:41 2014 Subject: [Histonet] RE: Carpal Tunnel In-Reply-To: <20140206172432.793E11E8066@trendmess-svr.holyredeemer.local> References: <20140206172432.793E11E8066@trendmess-svr.holyredeemer.local> Message-ID: I had bilateral carpal tunnel over 30 years ago, long before it was recognized as a problem associated with Histotechnology. I delayed seeking help, not recognizing the problem for what it was. When I finally had surgery, I had sustained permanent nerve damage. So, don't put off treatment! Both of my hands were done as open (there was no laparoscopic available way back then) and my recovery took 6wks before I could write my name, or turn a microtome wheel. It took a full 6 months before I was completely back to normal flexibility and grip strength for all activities including playing guitar and piano, grooming my own dog, using a weedeater. Holding items that vibrate (clippers, hairdryer) seemed to be the worse and they caution you to avoid them until completely healed. The good news is I've never had a problem since, however, I can't make the #3 sign with my hand as I lost the ability to touch thumb to little finger. My only regret is that I didn't do it sooner! Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- 1. Carpal tunnel (Angelicola,Lisa) Message: 1 Date: Wed, 5 Feb 2014 13:20:24 -0500 From: "Angelicola,Lisa" Subject: [Histonet] Carpal tunnel I have just been diagnosed with carpal tunnel in both of my hands and was wondering if any fellow histotechs have had this problem. I'm figuring it's a pretty common ailment in our line of work and after 31 years, it's caught up with me! I am considering surgery, but wanted to get any opinions on anyone that has gone through it, from a histotechs perspective. How long was the recovery, did it work for you, were you able to go back to work and continue to do what you did before. Do you any advice, suggestions, ect. I'd like to know the good, bad and the ugly! Thanks! Lisa Angelicola ************************* --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From 11z <@t> comcast.net Thu Feb 6 14:02:23 2014 From: 11z <@t> comcast.net (LeRoy Brown) Date: Thu Feb 6 14:02:41 2014 Subject: [Histonet] need protocol for Schiffs-Mallary trichrome Stain Message-ID: <001701cf2376$5a5581a0$0f0084e0$@comcast.net> Does anyone here do this Schiffs-Mallary trichrome technique and if so do you have a procedure you could share thanks, LeRoy Brown HT(ASCP) HTL 11z@comcast.net From dunatrsd <@t> sbcglobal.net Thu Feb 6 14:39:04 2014 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Thu Feb 6 14:39:20 2014 Subject: [Histonet] Chlamydia infected tissue In-Reply-To: <001701cf2376$5a5581a0$0f0084e0$@comcast.net> References: <001701cf2376$5a5581a0$0f0084e0$@comcast.net> Message-ID: <1391719144.23784.YahooMailNeo@web181704.mail.ne1.yahoo.com> Hi, Does anyone have chlamydia infected tissue, or info on where I might possibly get some? I need to optimize this antibody, but do not have positive control tissue.? Any info would be greatly appreciated. thank you Dusko Trajkovic From rheyna <@t> lumc.edu Thu Feb 6 15:23:49 2014 From: rheyna <@t> lumc.edu (Roger Heyna) Date: Thu Feb 6 15:24:07 2014 Subject: [Histonet] Accessioning Cut-off Times Message-ID: <52F3A905020000230008DB7E@gwgwia1.luhs.org> Hi Everyone, I'm curious about what time everyone stops accessioning specimens for the day. We receive specimens pretty late in the day, and we're evaluating our accessioning cut-off times to improve the TAT of these late specimens. We're focused more on biopsies, but we're also curious about large specimen types. Any feedback would be appreciated. Thanks, Roger Maywood, IL From vrivera <@t> westderm.com Thu Feb 6 16:01:15 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Thu Feb 6 16:01:21 2014 Subject: [Histonet] Internal audit Message-ID: <3D4A471B82E7A44C87F6839732320D9F046383@VSPDMS-ITEXMB02.DMS.COM> Hello once again fellow histonetters: I am looking to update our internal laboratory auditing procedures. Does anybody out there have a checklist they would like to share with me? Any help would be greatly appreciated. Thank you in advance, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) From liz <@t> premierlab.com Thu Feb 6 16:08:11 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Feb 6 16:08:15 2014 Subject: [Histonet] RE: Internal audit In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F046383@VSPDMS-ITEXMB02.DMS.COM> References: <3D4A471B82E7A44C87F6839732320D9F046383@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E052F4@SBS2K8.premierlab.local> Vincent Why don't you just use the CAP Checklist. I think it's available on the CAP website if you are a member. I may be wrong so I'm sure someone out there will correct me if I am. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vincent Rivera Sent: Thursday, February 06, 2014 3:01 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Internal audit Hello once again fellow histonetters: I am looking to update our internal laboratory auditing procedures. Does anybody out there have a checklist they would like to share with me? Any help would be greatly appreciated. Thank you in advance, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nowakc <@t> comcast.net Thu Feb 6 16:09:34 2014 From: nowakc <@t> comcast.net (nowakc@comcast.net) Date: Thu Feb 6 16:09:53 2014 Subject: [Histonet] Part time histology position available In-Reply-To: <1568247012.359720.1391724555997.JavaMail.root@comcast.net> Message-ID: <186753660.359833.1391724574242.JavaMail.root@comcast.net> Part time Histology Technician position available at Valley Gastroenterology in Spokane Valley, WA. Approximately 15-20 hours a week Monday through Friday and be available to work additional hours per week for coverage of personal time off for the full time histology technician. Day shift. No weekends. Closed on all major holidays. Needs to be proficient in accessioning, grossing, processing, embedding, cutting, staining and all other activities required for running a safe and efficient GI Histology Lab. Minimum Requirements/Education: HT or HTL (ASCP) certified, AAS Degree in Histotechnology, 2 years of Histotech experience, and be acquainted with CLIA and CAP regulations. Salary DOE; No relocation offered. Please submit CV or Resume with Cover letter to Corrine@valley-gi.com From vrivera <@t> westderm.com Thu Feb 6 16:12:48 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Thu Feb 6 16:12:57 2014 Subject: [Histonet] RE: Internal audit In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E052F4@SBS2K8.premierlab.local> References: <3D4A471B82E7A44C87F6839732320D9F046383@VSPDMS-ITEXMB02.DMS.COM> <14E2C6176416974295479C64A11CB9AE019C79E052F4@SBS2K8.premierlab.local> Message-ID: <3D4A471B82E7A44C87F6839732320D9F0463B3@VSPDMS-ITEXMB02.DMS.COM> Hi Liz, We have a copy of the CAP checklist at our lab. I was just curious to know how everyone else is going about their audits. Regards, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: Elizabeth Chlipala [mailto:liz@premierlab.com] Sent: Thursday, February 06, 2014 2:08 PM To: Vincent Rivera; 'histonet@lists.utsouthwestern.edu' Subject: RE: Internal audit Vincent Why don't you just use the CAP Checklist. I think it's available on the CAP website if you are a member. I may be wrong so I'm sure someone out there will correct me if I am. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vincent Rivera Sent: Thursday, February 06, 2014 3:01 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Internal audit Hello once again fellow histonetters: I am looking to update our internal laboratory auditing procedures. Does anybody out there have a checklist they would like to share with me? Any help would be greatly appreciated. Thank you in advance, Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Feb 6 16:51:27 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Feb 6 16:51:34 2014 Subject: [Histonet] RE: Internal audit In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F0463B3@VSPDMS-ITEXMB02.DMS.COM> References: <3D4A471B82E7A44C87F6839732320D9F046383@VSPDMS-ITEXMB02.DMS.COM>, <14E2C6176416974295479C64A11CB9AE019C79E052F4@SBS2K8.premierlab.local>, <3D4A471B82E7A44C87F6839732320D9F0463B3@VSPDMS-ITEXMB02.DMS.COM> Message-ID: I made my own based on ISO documents, but also the lab common, general, molecular, cyotgenetics, and AP checklists, and OSHA- since my services here span all those. I think it needs to be tailored to your lab. The important part to me are the corrective actions. There are general (non-customized) checklists on the website, I believe Elizabeth is correct. I think older ones are available to everyone. I have a schedule of every non-inspection year. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: vrivera@westderm.com > To: liz@premierlab.com; histonet@lists.utsouthwestern.edu > Date: Thu, 6 Feb 2014 22:12:48 +0000 > CC: > Subject: [Histonet] RE: Internal audit > > Hi Liz, > > We have a copy of the CAP checklist at our lab. I was just curious to know how everyone else is going about their audits. > > Regards, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: Elizabeth Chlipala [mailto:liz@premierlab.com] > Sent: Thursday, February 06, 2014 2:08 PM > To: Vincent Rivera; 'histonet@lists.utsouthwestern.edu' > Subject: RE: Internal audit > > Vincent > > Why don't you just use the CAP Checklist. I think it's available on the CAP website if you are a member. I may be wrong so I'm sure someone out there will correct me if I am. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vincent Rivera > Sent: Thursday, February 06, 2014 3:01 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Internal audit > > Hello once again fellow histonetters: > > I am looking to update our internal laboratory auditing procedures. Does anybody out there have a checklist they would like to share with me? Any help would be greatly appreciated. > > Thank you in advance, > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From roeeg <@t> migal.org.il Fri Feb 7 12:15:23 2014 From: roeeg <@t> migal.org.il (roee gutman) Date: Fri Feb 7 12:15:44 2014 Subject: [Histonet] fdssadfhgrw Message-ID: <8rjg7ffm1pb8rvq51nni164q.1391796919639@email.android.com> Best, Roee Gutman, PhD roeeg@migal.org.il, roeegu@telhai.ac.il 972-52-8422601 Sent from my Samsung From roeeg <@t> migal.org.il Fri Feb 7 12:15:27 2014 From: roeeg <@t> migal.org.il (roee gutman) Date: Fri Feb 7 12:15:45 2014 Subject: [Histonet] (no subject) Message-ID: Best, Roee Gutman, PhD roeeg@migal.org.il, roeegu@telhai.ac.il 972-52-8422601 Sent from my Samsung From BZIMMERM <@t> gru.edu Fri Feb 7 12:35:27 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Feb 7 12:35:36 2014 Subject: [Histonet] HISTOPALOOZA APRIL 25 - 27, 2014 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF80EB2A@EX-MLB-03.ad.georgiahealth.edu> The "Godfather" of Histology will be a featured speaker at our GSH meeting this year. Vinnie Della Speranza will be presenting two workshops on Saturday, April 26th. The first one is titled: Because Strangling Isn't an Option; Keeping Your Sanity While Dealing with the Problem Employee. This is very fitting for the Godfather, don't you think? ( I just hope I don't find a horse head in my hotel room that weekend). The second one is titled Communicating with Physicians and Administrators: Strategies and Tips for a Successful Outcome. I was privileged to hear the second presentation while attending NSH this past year. It was very informative and Vinnie offered wonderful tidbits that I took back to work with me. The Godfather has instructed me to tell everyone about the GSH website. Please secure your room reservation and don't miss an opportunity to network and have fun. Find a roomie to share costs. Tip: This should be someone you can tolerate in close proximity for about 72 hours. Stay tuned for more information about speakers in the upcoming weeks. From lcolbert <@t> pathmdlabs.com Fri Feb 7 13:03:05 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Fri Feb 7 13:05:26 2014 Subject: [Histonet] Billing for ASR's and RUO's Message-ID: <12ECD7346266D74691EC2BFC75285E452F3F9E30@BFL323E10.pathmdlabs.local> Do hospitals/labs get reimbursed for staining with ASR and RUO antibodies since they are not FDA-approved? What if they are validated in your own lab? Laurie Colbert, HT (ASCP) From vrivera <@t> westderm.com Fri Feb 7 17:06:10 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Fri Feb 7 17:06:22 2014 Subject: [Histonet] RE: Internal audit In-Reply-To: References: <3D4A471B82E7A44C87F6839732320D9F046383@VSPDMS-ITEXMB02.DMS.COM>, <14E2C6176416974295479C64A11CB9AE019C79E052F4@SBS2K8.premierlab.local>, <3D4A471B82E7A44C87F6839732320D9F0463B3@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <3D4A471B82E7A44C87F6839732320D9F046636@VSPDMS-ITEXMB02.DMS.COM> Thanks for everyone's input, I really appreciate the help. Have a great weekend! Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) From: joelle weaver [mailto:joelleweaver@hotmail.com] Sent: Thursday, February 06, 2014 2:51 PM To: Vincent Rivera; 'Elizabeth Chlipala'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] RE: Internal audit I made my own based on ISO documents, but also the lab common, general, molecular, cyotgenetics, and AP checklists, and OSHA- since my services here span all those. I think it needs to be tailored to your lab. The important part to me are the corrective actions. There are general (non-customized) checklists on the website, I believe Elizabeth is correct. I think older ones are available to everyone. I have a schedule of every non-inspection year. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: vrivera@westderm.com > To: liz@premierlab.com; histonet@lists.utsouthwestern.edu > Date: Thu, 6 Feb 2014 22:12:48 +0000 > CC: > Subject: [Histonet] RE: Internal audit > > Hi Liz, > > We have a copy of the CAP checklist at our lab. I was just curious to know how everyone else is going about their audits. > > Regards, > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > > -----Original Message----- > From: Elizabeth Chlipala [mailto:liz@premierlab.com] > Sent: Thursday, February 06, 2014 2:08 PM > To: Vincent Rivera; 'histonet@lists.utsouthwestern.edu' > Subject: RE: Internal audit > > Vincent > > Why don't you just use the CAP Checklist. I think it's available on the CAP website if you are a member. I may be wrong so I'm sure someone out there will correct me if I am. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vincent Rivera > Sent: Thursday, February 06, 2014 3:01 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Internal audit > > Hello once again fellow histonetters: > > I am looking to update our internal laboratory auditing procedures. Does anybody out there have a checklist they would like to share with me? Any help would be greatly appreciated. > > Thank you in advance, > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com> > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From halvo198 <@t> d.umn.edu Sat Feb 8 08:39:57 2014 From: halvo198 <@t> d.umn.edu (Leif Halvorson) Date: Sat Feb 8 08:40:04 2014 Subject: [Histonet] High Fat Tissue Processing Protocol? Message-ID: We are using histological analysis to characterize the distribution of fat cells and gonad development. We have attempted cryostat with no luck as there is too much unsaturated fatty acids and the fat never freezes. We are now attempting paraffin histo with some success, but we are having trouble with our tissue processing program. Can anyone share with us a tissue processing protocol for tissues that have high fat? Thank you so much. From Janine.Simms-Colon <@t> va.gov Mon Feb 10 09:38:20 2014 From: Janine.Simms-Colon <@t> va.gov (Simms-Colon, Janine) Date: Mon Feb 10 09:39:17 2014 Subject: [Histonet] VA LIS Message-ID: <66B3208B38DB8F4C9924077FE4E68ACAB6E8CCF47E@R04BYNMSGA1.r04.med.va.gov> Good morning all, To those working in the VA system, what LIS are you using? We are currently accessioning through Vista which if you work in the VA is used for many other processes such as timekeeping and ordering supplies but it is extremely archaic and not ideal for anatomic pathology, (in my humble opinion). Our barcoding is not interfaced with Vista and I am trying to convince management to upgrade to COPATH, WINDOWPATH, or POWERPATH, really anything other than what we have now. I have been told all VA Histology labs only use VISTA and I hope to God this is not true. So if anyone works at a VA, used to work there or know someone who works there, please contact me to let me know what you are using and how your process works. Thank you in advance. From Lauren.Richey <@t> tufts.edu Mon Feb 10 12:06:53 2014 From: Lauren.Richey <@t> tufts.edu (Richey, Lauren) Date: Mon Feb 10 12:07:02 2014 Subject: [Histonet] human research samples Message-ID: <7D36FC459047524E90E5EC68BE2A4208129D55D8@SSVMEXDAG01MB03.tufts.ad.tufts.edu> Hello Histonet, We have a small research animal histology core lab. We do not undergo CAP accreditation. We have only accepted animal tissues with the exception of xenografts. Recently we have received a few requests to process and section deidentified human tissue classified by the IRB as tissue that "does not constitute human subject research." Our histologists are HT and HTL certified, have prior experience in a human clinical lab, and are skilled at sectioning the types of human tissues that are being requested. I am a veterinary pathologist. My questions never having worked with human tissue before, and out of concern for following rules and proper safety practices: -Is there a reason our lab should not accept human tissue for research after review by the IRB? -Does having human tissue in the lab for research purposes have any additional regulatory issues (besides anything that might be covered by IRB or IBC review). -We have always accepted animal tissues that contained xenografts from humans, whether from a cell line or from a primary tumor. Now, reconsidering a general policy on human tissue in the lab, is there any documentation or special handling/clean up that you recommend after receipt and sectioning of human tissues in a research lab? Should we ask to retain a copy of the IRB approval if a primary tumor xenograft is used in an animal and comes to us? And, although we treat all tissues as potentially biohazardous, are there any particular extra precautions to take, for example, after cryosectioning human tissue? Thank you, Lauren Lauren Richey, DVM, PhD, Diplomate ACVP Director, Tufts Comparative Pathology Services Assistant Director, DLAM Tufts University Boston, MA 02111 617-636-6488 http://sites.tufts.edu/histopath/ From BZIMMERM <@t> gru.edu Mon Feb 10 15:34:18 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Mon Feb 10 15:38:14 2014 Subject: [Histonet] HISTOPALOOZA April 25 - 27 The Lodge and Spa at Callaway Gardens Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF80F836@EX-MLB-03.ad.georgiahealth.edu> I'm back with another tidbit about our upcoming meeting at Callaway Gardens. Robert Lott, NSH 2010 Histotech of the Year, will be conducting a workshop on Certification Readiness. He will be giving a thorough overview/explanation of the Computer Adaptive Testing (CAT) process and a great review of the subject matter. A study outline will be provided which was published by the Board of Certification. There will be extensive handout materials for folks enrolled in the workshop. This is a great review for the self-study or OJT student. I could say this is like a histonet infomercial, but I can't promise you flat abs in 30 days or that this class will cure baldness. However, if you are enrolled in this class, there's a significant chance you will pass the HT/HTL exam with or without your muffin top. From Haley.Huggins <@t> DignityHealth.org Mon Feb 10 18:07:06 2014 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Mon Feb 10 18:07:15 2014 Subject: [Histonet] Purple haze on H&E slides Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA22@PHX-MSG-007-N2.chw.edu> I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world. Haley Huggins From tmcampbell <@t> fmh.org Mon Feb 10 19:37:37 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Mon Feb 10 19:37:51 2014 Subject: [Histonet] Purple haze on H&E slides In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA22@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA22@PHX-MSG-007-N2.chw.edu> Message-ID: What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath? Tasha Sent from my iPhone > On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" wrote: > > I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world. > > Haley Huggins > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis <@t> che-east.org Tue Feb 11 09:00:52 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Tue Feb 11 09:02:08 2014 Subject: [Histonet] RE: Histonet Digest, Vol 123, Issue 10 In-Reply-To: <25071f2f-f2a5-4029-8de8-d661916a0b72@CHESXH02.one.ads.che.org> References: <25071f2f-f2a5-4029-8de8-d661916a0b72@CHESXH02.one.ads.che.org> Message-ID: <08861B9CF6C7774E874635A4818AE37B088180C7F9@CHEXCMS01.one.ads.che.org> That's what was used at the Wilmington VA and (I think) Philadelphia VA too. Cassandra Davis CDavis@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu [histonet-request@lists.utsouthwestern.edu] Sent: Monday, February 10, 2014 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 123, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. VA LIS (Simms-Colon, Janine) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Feb 2014 10:38:20 -0500 From: "Simms-Colon, Janine" Subject: [Histonet] VA LIS To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <66B3208B38DB8F4C9924077FE4E68ACAB6E8CCF47E@R04BYNMSGA1.r04.med.va.gov> Content-Type: text/plain; charset="us-ascii" Good morning all, To those working in the VA system, what LIS are you using? We are currently accessioning through Vista which if you work in the VA is used for many other processes such as timekeeping and ordering supplies but it is extremely archaic and not ideal for anatomic pathology, (in my humble opinion). Our barcoding is not interfaced with Vista and I am trying to convince management to upgrade to COPATH, WINDOWPATH, or POWERPATH, really anything other than what we have now. I have been told all VA Histology labs only use VISTA and I hope to God this is not true. So if anyone works at a VA, used to work there or know someone who works there, please contact me to let me know what you are using and how your process works. Thank you in advance. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 123, Issue 10 ***************************************** Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Shannon.Burrage <@t> unitypoint.org Tue Feb 11 09:14:36 2014 From: Shannon.Burrage <@t> unitypoint.org (Burrage, Shannon L.) Date: Tue Feb 11 09:14:44 2014 Subject: [Histonet] Purple haze on H&E slides In-Reply-To: References: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA22@PHX-MSG-007-N2.chw.edu>, Message-ID: <8E6784497F13AC44A38383BCB11826AD7B472634@TDCEXCHXM007.ihs.org> Are you using acid alcohol, and Harris Hematoxylin? Are you filtering the heamatatoxylin every day? We still use a very small amount of gelatin in the waerbath. And get no background stain. So many veriables here. Good luck, Shannon Burrage shannon.burrage@unitypoint.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Campbell, Tasha M. [tmcampbell@fmh.org] Sent: Monday, February 10, 2014 7:37 PM To: Huggins, Haley - MRMC Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple haze on H&E slides What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath? Tasha Sent from my iPhone > On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" wrote: > > I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world. > > Haley Huggins > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Shannon.Burrage <@t> unitypoint.org Tue Feb 11 09:50:57 2014 From: Shannon.Burrage <@t> unitypoint.org (Burrage, Shannon L.) Date: Tue Feb 11 09:51:04 2014 Subject: [Histonet] RE: Part time histology position available In-Reply-To: <186753660.359833.1391724574242.JavaMail.root@comcast.net> References: <1568247012.359720.1391724555997.JavaMail.root@comcast.net>, <186753660.359833.1391724574242.JavaMail.root@comcast.net> Message-ID: <8E6784497F13AC44A38383BCB11826AD7B473695@TDCEXCHXM007.ihs.org> Good morning all: While we are talking about job opportunities, we at Allen Hosptial in Waterloo, Iowa are losing a bright, inventive and enjoyable co-worker to family in Colorado Springs. Our co-worker is not a registered histotech. She is well educated and a great histotech never the less. Anyone know of any opportunities for our lose? Thanks; Shannon Burrage HTL(ASCP) histology supervisor Allen Hospital 1825 Logan Avenue, Waterloo, Iowa 50703 319-235-5057 shannon.burrage@unitypoint.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of nowakc@comcast.net [nowakc@comcast.net] Sent: Thursday, February 06, 2014 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Part time histology position available Part time Histology Technician position available at Valley Gastroenterology in Spokane Valley, WA. Approximately 15-20 hours a week Monday through Friday and be available to work additional hours per week for coverage of personal time off for the full time histology technician. Day shift. No weekends. Closed on all major holidays. Needs to be proficient in accessioning, grossing, processing, embedding, cutting, staining and all other activities required for running a safe and efficient GI Histology Lab. Minimum Requirements/Education: HT or HTL (ASCP) certified, AAS Degree in Histotechnology, 2 years of Histotech experience, and be acquainted with CLIA and CAP regulations. Salary DOE; No relocation offered. Please submit CV or Resume with Cover letter to Corrine@valley-gi.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From imhyper13 <@t> aol.com Tue Feb 11 09:58:39 2014 From: imhyper13 <@t> aol.com (imhyper13@aol.com) Date: Tue Feb 11 09:58:42 2014 Subject: [Histonet] Does anyone have experience?? Message-ID: <8D0F559435A1172-FA8-1F317@webmail-d269.sysops.aol.com> Hi, I was wondering if anyone has experience with or is presently using Soft Path Dx? If so, what are your thoughts on the application. Thank you From relia1 <@t> earthlink.net Tue Feb 11 10:07:07 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Feb 11 10:07:14 2014 Subject: [Histonet] RELIA Histology Careers Bulletin 2-11-2014 Happy Valentines' Day - Sweets for the Sweet and some exciting new opportunities Message-ID: <001701cf2743$504142a0$f0c3c7e0$@earthlink.net> Hi Histonetters!! I hope this is the start to an especially sweet week since Friday is Valentines? Day. Happy Valentines? Day!!! I have some great histology opportunities to tell you about. Please feel free to take a second and peruse the list kind of the way one peruses an Assortment of chocolates in a heart shaped box! All of these are permanent full time positions and our clients offer excellent compensation, benefits and in some cases relocation and or sign on bonuses. My personal favorites are Chocolate Covered Cherries and French Macarons!! How about you? Here is a list of my current openings!! Let me know if anything looks good!! Management: Pathology Supervisor ? Cape Cod, MA Supervise histos and PAs Histology Supervisor ? Atlanta, GA- Busy Hospital Lab HT/HTL Opportunities: Histotech with Grossing exper. Boston, MA ?day and night shifts Histotechnologist w/FISH ? Louisville, KY-join a leading edge lab! Dermpath Histotech ? Atlanta, GA private lab evening shift Other Opportunities Grossing Tech ? Austin, TX Cytotech w/histo experience? Lafayette, LA If you are interested in any of these positions please call me on my cell at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1@earthlink.net **If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. **If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. **I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open EVEN IF you are happy in your present job. Valentines? Day Special: If you refer someone that I place I will pay you a referral fee & during the month of February I will throw in a box of Godiva Chocolates!!! Have a wonderful Valentine?s Day!! Thanks-Pam Thank you, Pam ? 866-607-3542 (866-60RELIA) 407-353-5070-cell Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From NGUPTA1 <@t> hfhs.org Tue Feb 11 10:23:22 2014 From: NGUPTA1 <@t> hfhs.org (Gupta, Nilesh) Date: Tue Feb 11 10:23:27 2014 Subject: [Histonet] Buffer pH monitoring Message-ID: I just want to get an idea of how often labs monitor the pH of the buffers used in IHC labs. Do you pH for every bottleof a lot received prior to use, single bottle of a lot only or do you have to pH every time you reconstitute the buffer. It is a CAP checklist question for AP & here is how the checklist question is worded. It says when a new "batch" is prepared or received. Any input is greatly appreciated. Thanks Nilesh Gupta, M.D, Henry Ford Hospital, Detroit, MI The pH of the buffers used in immunohistochemistry is routinely monitored. NOTE: pH must be tested when a new batch is prepared or received. Evidence of Compliance: ? Written procedure defining pH range for each buffer in use AND ? Records of initial and subsequent QC on each buffer ________________________________ CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy & Security page on www.henryford.com for more detailed information as well as information concerning MyChart, our new patient portal. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. From blayjorge <@t> gmail.com Tue Feb 11 10:24:49 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Tue Feb 11 10:24:53 2014 Subject: [Histonet] Py/GC/MS Message-ID: Hello: Can someone recommend me laboratories equipped with Py/GC/MS? I have many samples of cellular products needing to have analyzed. If you have one or more recommendations, please feel to email them directly to me. Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From billodonnell <@t> catholichealth.net Tue Feb 11 10:28:07 2014 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Feb 11 10:28:22 2014 Subject: [Histonet] Bio banking ? Message-ID: OK, our institution has embraced the idea of bio banking, but we do not have one on site. We have a person who consents the patient, delivers the specimen to the lab, puts the tissue in cassettes that she provides. We in histology will embed, cut one slide per block, stain and hold the slides and blocks for this person to pick up. There has been some miscommunication. I thought we were getting paid by the bio bank firm to do this, the bio bank person also assumed we were being paid - but we have not been. The bio bank firm agrees that a fee should be paid us - but we have no idea what to charge. We want it to be a reasonable transaction between us. Is anyone else charging for this service? If so, what do you charge per block or per case? Any help would be appreciated! William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From Haley.Huggins <@t> DignityHealth.org Tue Feb 11 11:06:51 2014 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Tue Feb 11 11:06:58 2014 Subject: [Histonet] Purple haze on H&E slides In-Reply-To: References: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA22@PHX-MSG-007-N2.chw.edu> Message-ID: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA89@PHX-MSG-007-N2.chw.edu> We are using the Leica Selectech brand. No additives to the waterbath. We are using xtra or superfrost slides. The protocol is as follows: 3 min xylene 3 min xylene 1 min 100% ETOH 2 min 100% ETOH 1 min 95% ETOH 1 min water rinse 4 min hematoxylin 2 min water rinse 15 sec acid alcohol 1 min water rinse 1.5 min bluing 1 min water rinse 1 min 95% ETOH 5 min eosin 30 sec 100% ETOH 1 min 100% ETOH 1 min 100% ETOH 1 min xylene 2 min xylene Haley -----Original Message----- From: Campbell, Tasha M. [mailto:tmcampbell@fmh.org] Sent: Monday, February 10, 2014 5:38 PM To: Huggins, Haley - MRMC Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple haze on H&E slides What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath? Tasha Sent from my iPhone > On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" wrote: > > I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world. > > Haley Huggins > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abuchiane <@t> bmhvt.org Tue Feb 11 11:25:38 2014 From: abuchiane <@t> bmhvt.org (Anita Buchiane) Date: Tue Feb 11 11:25:43 2014 Subject: [Histonet] Purple haze on H&E slides In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA89@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA22@PHX-MSG-007-N2.chw.edu> <4F36EC93A5737D4F8A2974E8FB8E260616028FDA89@PHX-MSG-007-N2.chw.edu> Message-ID: <4034E71604330C4B8E10D1538DFB2455C28B6ED8@BMHEXCH02.bmhvt.org> It looks to me like the problem might be caused by inadequate dehydration after the eosin. Add a 95 % after the eosin then 3 100 %'s then the Xylenes. 2 min. in all of these should be adequate. Hope this helps Anita -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Huggins, Haley - MRMC Sent: Tuesday, February 11, 2014 12:07 PM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple haze on H&E slides We are using the Leica Selectech brand. No additives to the waterbath. We are using xtra or superfrost slides. The protocol is as follows: 3 min xylene 3 min xylene 1 min 100% ETOH 2 min 100% ETOH 1 min 95% ETOH 1 min water rinse 4 min hematoxylin 2 min water rinse 15 sec acid alcohol 1 min water rinse 1.5 min bluing 1 min water rinse 1 min 95% ETOH 5 min eosin 30 sec 100% ETOH 1 min 100% ETOH 1 min 100% ETOH 1 min xylene 2 min xylene Haley -----Original Message----- From: Campbell, Tasha M. [mailto:tmcampbell@fmh.org] Sent: Monday, February 10, 2014 5:38 PM To: Huggins, Haley - MRMC Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple haze on H&E slides What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath? Tasha Sent from my iPhone > On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" wrote: > > I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world. > > Haley Huggins > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ From lblazek <@t> digestivespecialists.com Tue Feb 11 12:13:03 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Feb 11 12:13:08 2014 Subject: [Histonet] Purple haze on H&E slides In-Reply-To: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA89@PHX-MSG-007-N2.chw.edu> References: <4F36EC93A5737D4F8A2974E8FB8E260616028FDA22@PHX-MSG-007-N2.chw.edu> <4F36EC93A5737D4F8A2974E8FB8E260616028FDA89@PHX-MSG-007-N2.chw.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E39167FE4470E@IBMB7Exchange.digestivespecialists.com> Haley, We use Leica's Selectech also and occasionally have the same issue. I don't think your ETOH after the eosin is a problem. If you add a 95% you will leach out the eosin a bit. I think you should add another 15 - 30 seconds in the acid alcohol. One of the things we find here though is that there can be a great variation to the pH of the water. That affects the results of the stain. Specifically the "purple haze" Go Jimi! Purple haze all in my brain Lately things just don't seem the same Actin' funny, but I don't know why 'Scuse me while I kiss the sky Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Huggins, Haley - MRMC Sent: Tuesday, February 11, 2014 12:07 PM To: Campbell, Tasha M. Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Purple haze on H&E slides We are using the Leica Selectech brand. No additives to the waterbath. We are using xtra or superfrost slides. The protocol is as follows: 3 min xylene 3 min xylene 1 min 100% ETOH 2 min 100% ETOH 1 min 95% ETOH 1 min water rinse 4 min hematoxylin 2 min water rinse 15 sec acid alcohol 1 min water rinse 1.5 min bluing 1 min water rinse 1 min 95% ETOH 5 min eosin 30 sec 100% ETOH 1 min 100% ETOH 1 min 100% ETOH 1 min xylene 2 min xylene Haley -----Original Message----- From: Campbell, Tasha M. [mailto:tmcampbell@fmh.org] Sent: Monday, February 10, 2014 5:38 PM To: Huggins, Haley - MRMC Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple haze on H&E slides What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath? Tasha Sent from my iPhone > On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" wrote: > > I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world. > > Haley Huggins > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sherrian.McAnn <@t> va.gov Tue Feb 11 12:33:25 2014 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Tue Feb 11 12:34:02 2014 Subject: [EXTERNAL] [Histonet] VA LIS Message-ID: <61E2B58CECEF384094A363989D47C0900AA6C925@VHAV17MSGA2.v17.med.va.gov> It's true and I do know that we are currently in process of upgrading to barcode reading ability. I don't know if this applies to all areas of our hospital but have heard rumors that it is happening. It's just seems to be a slow process. LOL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Simms-Colon, Janine Sent: Monday, February 10, 2014 9:38 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [EXTERNAL] [Histonet] VA LIS Good morning all, To those working in the VA system, what LIS are you using? We are currently accessioning through Vista which if you work in the VA is used for many other processes such as timekeeping and ordering supplies but it is extremely archaic and not ideal for anatomic pathology, (in my humble opinion). Our barcoding is not interfaced with Vista and I am trying to convince management to upgrade to COPATH, WINDOWPATH, or POWERPATH, really anything other than what we have now. I have been told all VA Histology labs only use VISTA and I hope to God this is not true. So if anyone works at a VA, used to work there or know someone who works there, please contact me to let me know what you are using and how your process works. Thank you in advance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sherrian.McAnn <@t> va.gov Tue Feb 11 12:36:30 2014 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Tue Feb 11 12:36:57 2014 Subject: [EXTERNAL] RE: [Histonet] Purple haze on H&E slides Message-ID: <61E2B58CECEF384094A363989D47C0900AA6C92B@VHAV17MSGA2.v17.med.va.gov> We once had this problem..We routinely filter our hematoxylin every morning at startup. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burrage, Shannon L. Sent: Tuesday, February 11, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: [Histonet] Purple haze on H&E slides Are you using acid alcohol, and Harris Hematoxylin? Are you filtering the heamatatoxylin every day? We still use a very small amount of gelatin in the waerbath. And get no background stain. So many veriables here. Good luck, Shannon Burrage shannon.burrage@unitypoint.org ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Campbell, Tasha M. [tmcampbell@fmh.org] Sent: Monday, February 10, 2014 7:37 PM To: Huggins, Haley - MRMC Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Purple haze on H&E slides What brand of reagents are u using and what is your protocol? What slides r u using and r u using gelatin in ur waterbath? Tasha Sent from my iPhone > On Feb 10, 2014, at 7:10 PM, "Huggins, Haley - MRMC" wrote: > > I am trying to get the histology lab up and running and I am having difficulties getting the H&E Stain just right. I am nearly there, but I keep getting a purple bleed or haze around the tissue. Any suggestions on getting rid of this? I actually got rid of it at one point, but the medical director wasn't happy with the stain. I appreciate any suggestions from the histology world. > > Haley Huggins > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sherrian.McAnn <@t> va.gov Tue Feb 11 12:45:34 2014 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Tue Feb 11 12:46:07 2014 Subject: [EXTERNAL] [Histonet] Blocks cracking In-Reply-To: <7390ec9c1df6.52f25df2@usc.edu> References: <7390ec9c1df6.52f25df2@usc.edu> Message-ID: <61E2B58CECEF384094A363989D47C0900AA6C939@VHAV17MSGA2.v17.med.va.gov> We too are having that problem. I had heard that the paraffin had been changed and now we get cracks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Erica Canal Sent: Wednesday, February 05, 2014 5:51 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Blocks cracking Hello all, We are wondering harder paraffin makes blocks more fragile. We have been using Leica's Formula R and on a few occasions & when we have mailed blocks out, we receive them back in pieces. Could this be because of type of wax we are using? The quality of our slides is great, we just seem to be having a problem lately with our blocks breaking after they have been sent out & we want to try to prevent that. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TanyaAbbott <@t> catholichealth.net Tue Feb 11 12:53:32 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Tue Feb 11 12:53:36 2014 Subject: [Histonet] Negative Controls for IHC Message-ID: <852F7D2C14FB464D80E182B15DB138AF2D7FC772@CHIEX005.CHI.catholichealth.net> I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From thigginsht <@t> msn.com Tue Feb 11 12:54:01 2014 From: thigginsht <@t> msn.com (Tim Higgins) Date: Tue Feb 11 12:54:06 2014 Subject: [Histonet] RE: Buffer pH monitoring In-Reply-To: References: Message-ID: I check pH every time I make a new batch(bottle) and then periodically there after. Timothy N. Higgins, HT (ASCP), QIHC > Message: 10 > Date: Tue, 11 Feb 2014 16:23:22 +0000 > From: "Gupta, Nilesh" > Subject: [Histonet] Buffer pH monitoring > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > Content-Type: text/plain; charset="utf-8" > > I just want to get an idea of how often labs monitor the pH of the buffers used in IHC labs. Do you pH for every bottleof a lot received prior to use, single bottle of a lot only or do you have to pH every time you reconstitute the buffer. > > It is a CAP checklist question for AP & here is how the checklist question is worded. It says when a new "batch" is prepared or received. Any input is greatly appreciated. Thanks Nilesh Gupta, M.D, Henry Ford Hospital, Detroit, MI > > The pH of the buffers used in immunohistochemistry is routinely monitored. > > NOTE: pH must be tested when a new batch is prepared or received. > > Evidence of Compliance: > > ??? Written procedure defining pH range for each buffer in use AND > > ??? Records of initial and subsequent QC on each buffer > > > > ________________________________ > > CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. > > Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy & Security page on www.henryford.com for more detailed information as well as information concerning MyChart, our new patient portal. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. From ahc53 <@t> georgetown.edu Tue Feb 11 13:44:05 2014 From: ahc53 <@t> georgetown.edu (Anna Coffey) Date: Tue Feb 11 13:44:09 2014 Subject: [Histonet] Troubleshooting Masson Trichrome Stain Message-ID: Hello Histonetters, We regularly perform the Masson Trichrome stain in our lab and recently have been noticing some inconsistent results. The main issue seems to be that the Biebrich Scarlet is not being adequately removed by the PTA/PMA, as many of the areas containing collagen are showing up as purple instead of bright blue. We use fresh reagents every time we run the stain and our protocols are as follows: Rehydrate (xylene --> 100%EtOH --> 95% --> 80% --> 70%) Bouin's in microwave (1 min) Stand at RT in Bouin's (15 min) Running tap (5 min) Weigert's Hx (10 min) Running Tap (5 min) Biebrich Scarlet (1.25 min) Rinse dH2O Phosphotungstic acid/Phosphomolybdic acid (10 min) Aniline Blue (10 min) Rinse dH2O 1% Acetic acid (1 min) Rinse dH2O Air dry for dehydration Has anyone else experienced this same problem and, if so, what corrective actions have worked for you? I'd greatly appreciate your advice! Thanks, Anna -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu From LSebree <@t> uwhealth.org Tue Feb 11 13:44:37 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Feb 11 13:44:46 2014 Subject: [Histonet] RE: Negative Controls for IHC In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF2D7FC772@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF2D7FC772@CHIEX005.CHI.catholichealth.net> Message-ID: <77DD817201982748BC67D7960F2F76AF099575@UWHC-MBX12.uwhis.hosp.wisc.edu> Hi Tanya, We have made the decision to continue running negative reagent control slides. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, February 11, 2014 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls for IHC I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Feb 11 13:53:23 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Feb 11 13:53:29 2014 Subject: [Histonet] RE: Buffer pH monitoring In-Reply-To: References: , Message-ID: Same here. Each new bottle ( whether new lot or not, interpret as "batch"). Record pH on a QC sheet. It is nearly always in range being proprietary, unless user/maker error. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: thigginsht@msn.com > To: histonet@lists.utsouthwestern.edu; ngupta1@hfhs.org > Date: Tue, 11 Feb 2014 12:54:01 -0600 > CC: > Subject: [Histonet] RE: Buffer pH monitoring > > I check pH every time I make a new batch(bottle) and then periodically there after. > > Timothy N. Higgins, HT (ASCP), QIHC > > > > Message: 10 > > Date: Tue, 11 Feb 2014 16:23:22 +0000 > > From: "Gupta, Nilesh" > > Subject: [Histonet] Buffer pH monitoring > > To: "'histonet@lists.utsouthwestern.edu'" > > > > Message-ID: > > > > Content-Type: text/plain; charset="utf-8" > > > > I just want to get an idea of how often labs monitor the pH of the buffers used in IHC labs. Do you pH for every bottleof a lot received prior to use, single bottle of a lot only or do you have to pH every time you reconstitute the buffer. > > > > It is a CAP checklist question for AP & here is how the checklist question is worded. It says when a new "batch" is prepared or received. Any input is greatly appreciated. Thanks Nilesh Gupta, M.D, Henry Ford Hospital, Detroit, MI > > > > The pH of the buffers used in immunohistochemistry is routinely monitored. > > > > NOTE: pH must be tested when a new batch is prepared or received. > > > > Evidence of Compliance: > > > > ??? Written procedure defining pH range for each buffer in use AND > > > > ??? Records of initial and subsequent QC on each buffer > > > > > > > > ________________________________ > > > > CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. > > > > Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy & Security page on www.henryford.com for more detailed information as well as information concerning MyChart, our new patient portal. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Feb 11 13:54:57 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Feb 11 13:55:00 2014 Subject: [Histonet] RE: Negative Controls for IHC In-Reply-To: <77DD817201982748BC67D7960F2F76AF099575@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <852F7D2C14FB464D80E182B15DB138AF2D7FC772@CHIEX005.CHI.catholichealth.net>, <77DD817201982748BC67D7960F2F76AF099575@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Not too long ago, there was a posting about labs that had discontinued. You technically don't need with a polymer, non-biotin system. I still do them- for now. The pathologist never uses them, internal controls only. But I do, but this may be a good cost cutting idea for the future. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: LSebree@uwhealth.org > To: TanyaAbbott@catholichealth.net; histonet@lists.utsouthwestern.edu > Date: Tue, 11 Feb 2014 19:44:37 +0000 > CC: > Subject: [Histonet] RE: Negative Controls for IHC > > Hi Tanya, > > We have made the decision to continue running negative reagent control slides. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya > Sent: Tuesday, February 11, 2014 12:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Negative Controls for IHC > > I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Feb 11 14:02:00 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Feb 11 14:02:13 2014 Subject: FW: [Histonet] Troubleshooting Masson Trichrome Stain References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E053C3@SBS2K8.premierlab.local> Sorry forgot to reply all. Anna Do you make up your solutions fresh especially the PTA/PMA and aniline blue. We don't run the microwave method we heat in an oven in bouins for 1 hour, but we make sure that the bouins is at 60C prior to us placing the slides in the solution. We stain longer in the Biebrich scarlet (20 minutes) we only use the PTA/PMA once and then about 2 minutes in the Aniline blue. We found that it's important to use fresh reagents, we get the best consistency of staining with all over fresh reagents. Since you are moving from the PTA/PMA right into the aniline blue, if you use the aniline blue over and over again the pH will change because you are carrying some PTA/PMA over on the slides, this will cause problems with the stain. We are not like a clinical lab so we could staining up to 100 or so trichrome slides at a time so making sure we have fresh reagents is key for consistency for our samples. Good Luck, one other thing the trichrome stain is one of the more difficult histology stains to run correctly. A good way to tell if the stain has worked properly is to look at smaller vessels. The smooth muscle of the smaller vessels should be red, and the connective tissue blue, frequently you will see the smooth muscle of the vessels stain a bit grey that's when you know that that stain has not worked. The stain needs to be balanced with neither component overpowering the other. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Coffey Sent: Tuesday, February 11, 2014 12:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Troubleshooting Masson Trichrome Stain Hello Histonetters, We regularly perform the Masson Trichrome stain in our lab and recently have been noticing some inconsistent results. The main issue seems to be that the Biebrich Scarlet is not being adequately removed by the PTA/PMA, as many of the areas containing collagen are showing up as purple instead of bright blue. We use fresh reagents every time we run the stain and our protocols are as follows: Rehydrate (xylene --> 100%EtOH --> 95% --> 80% --> 70%) Bouin's in microwave (1 min) Stand at RT in Bouin's (15 min) Running tap (5 min) Weigert's Hx (10 min) Running Tap (5 min) Biebrich Scarlet (1.25 min) Rinse dH2O Phosphotungstic acid/Phosphomolybdic acid (10 min) Aniline Blue (10 min) Rinse dH2O 1% Acetic acid (1 min) Rinse dH2O Air dry for dehydration Has anyone else experienced this same problem and, if so, what corrective actions have worked for you? I'd greatly appreciate your advice! Thanks, Anna -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michele.french <@t> bms.com Tue Feb 11 15:41:03 2014 From: michele.french <@t> bms.com (French, Michele) Date: Tue Feb 11 15:41:13 2014 Subject: [Histonet] NJ Histology Meeting Message-ID: <1A7E70D2A397674BA82830AAB9C5020D4FCC59272D@ushpwbmsmmp006.one.ads.bms.com> The New Jersey Society for Histotechnology would like to invite anyone within driving distance of Mount Laurel, NJ to our upcoming spring educational event on Saturday, April 5th. The agenda includes four 90 minute seminars: Respiratory Histopathology, Pathology of the Urinary Tract, Animal Model Development to Aid in the Understanding of Human Atherosclerosis, and Predictive Testing in Non-small Cell Lung Cancer: Testing Protocols, Specimen Requirements and Near Future Techniques, Notably Next Generation Sequencing. You can get more details and register by visiting our website http://njsh.org/njsh/ ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From amosbrooks <@t> gmail.com Tue Feb 11 16:11:48 2014 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Feb 11 16:11:56 2014 Subject: [Histonet] Buffer pH Message-ID: Hi, I'm worried that my opinion on this matter may be in a minority. I truly hope to be proved wrong. Every bottle of every lot should be tested for pH. This is especially true if you are doing any reconstituting of the buffers. I do not know if CAP actually looks for this, but they should considering all the other seemingly insignificant things they do look for. (Float bath temps... really?) If you reconstitute buffers with distilled water, the water pH varies and hence the buffer pH will vary some with it. Even if you don't and are willing to spend God knows how much to have ready to use buffer shipped to you, how sure are you that there is never any fluctuations in the pH from the factory or due to freezing on the loading docks. It is just a good practice to be 100% sure about this and it really doesn't take that long to check. Since checking the pH should be incorporated all the time,why not just make up the buffers ourselves anyway and save the 400% markup per bottle. (Yes I actually did the math on that the numbers are right!) So save money and increase accuracy while ensuring test quality by doing things the good old fashioned way. Alright will someone help this crotchety old git off his soapbox, Amos From joelleweaver <@t> hotmail.com Tue Feb 11 18:05:55 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Feb 11 18:06:01 2014 Subject: [Histonet] Buffer pH In-Reply-To: References: Message-ID: can't do that, because I think you are right. I do use a vendor's buffer that I reconstitute, and I check the pH every time. I keep a log of the reading and lot. Maybe CAP will or won't look for it, but I figure if they made a checklist item for it, it's expected. They focus on all the variables affecting testing reproducibility, so this just fits in with that approach. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Tue, 11 Feb 2014 17:11:48 -0500 From: amosbrooks@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Buffer pH Hi, I'm worried that my opinion on this matter may be in a minority. I truly hope to be proved wrong. Every bottle of every lot should be tested for pH. This is especially true if you are doing any reconstituting of the buffers. I do not know if CAP actually looks for this, but they should considering all the other seemingly insignificant things they do look for. (Float bath temps... really?) If you reconstitute buffers with distilled water, the water pH varies and hence the buffer pH will vary some with it. Even if you don't and are willing to spend God knows how much to have ready to use buffer shipped to you, how sure are you that there is never any fluctuations in the pH from the factory or due to freezing on the loading docks. It is just a good practice to be 100% sure about this and it really doesn't take that long to check. Since checking the pH should be incorporated all the time,why not just make up the buffers ourselves anyway and save the 400% markup per bottle. (Yes I actually did the math on that the numbers are right!) So save money and increase accuracy while ensuring test quality by doing things the good old fashioned way. Alright will someone help this crotchety old git off his soapbox, Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Wed Feb 12 08:04:43 2014 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Feb 12 08:07:38 2014 Subject: [Histonet] RE: Negative Controls for IHC In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF2D7FC772@CHIEX005.CHI.catholichealth.net>, <77DD817201982748BC67D7960F2F76AF099575@UWHC-MBX12.uwhis.hosp.wisc.edu>, Message-ID: I just stopped using the reagent controls on all our IHC due to the drastic cuts in reimbursement. It will be a significant immediate savings for our tiny lab. Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver [joelleweaver@hotmail.com] Sent: Tuesday, February 11, 2014 1:54 PM To: Sebree Linda A; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Negative Controls for IHC Not too long ago, there was a posting about labs that had discontinued. You technically don't need with a polymer, non-biotin system. I still do them- for now. The pathologist never uses them, internal controls only. But I do, but this may be a good cost cutting idea for the future. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: LSebree@uwhealth.org > To: TanyaAbbott@catholichealth.net; histonet@lists.utsouthwestern.edu > Date: Tue, 11 Feb 2014 19:44:37 +0000 > CC: > Subject: [Histonet] RE: Negative Controls for IHC > > Hi Tanya, > > We have made the decision to continue running negative reagent control slides. > > Linda A. Sebree > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya > Sent: Tuesday, February 11, 2014 12:54 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Negative Controls for IHC > > I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From joelleweaver <@t> hotmail.com Wed Feb 12 10:04:29 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Feb 12 10:04:32 2014 Subject: [Histonet] RE: Negative Controls for IHC In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF2D7FC772@CHIEX005.CHI.catholichealth.net>, , <77DD817201982748BC67D7960F2F76AF099575@UWHC-MBX12.uwhis.hosp.wisc.edu>, , Message-ID: Cheryl Yes, I understand. It is highly likely this will be the way things go here also. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: cmiller@physlab.com > To: joelleweaver@hotmail.com > CC: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu > Date: Wed, 12 Feb 2014 08:04:43 -0600 > Subject: RE: [Histonet] RE: Negative Controls for IHC > > I just stopped using the reagent controls on all our IHC due to the drastic cuts in reimbursement. It will be a significant immediate savings for our tiny lab. > Cheryl A. Miller HT ASCP cm > Histology Supervisor > Hygiene Officer > Physicians Laboratory, P.C. > 4840 F St. > Omaha , NE. 68117 > 402 731 4145 ext. 532 > Cell 402 493 0403 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver [joelleweaver@hotmail.com] > Sent: Tuesday, February 11, 2014 1:54 PM > To: Sebree Linda A; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Negative Controls for IHC > > Not too long ago, there was a posting about labs that had discontinued. You technically don't need with a polymer, non-biotin system. I still do them- for now. The pathologist never uses them, internal controls only. But I do, but this may be a good cost cutting idea for the future. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: LSebree@uwhealth.org > > To: TanyaAbbott@catholichealth.net; histonet@lists.utsouthwestern.edu > > Date: Tue, 11 Feb 2014 19:44:37 +0000 > > CC: > > Subject: [Histonet] RE: Negative Controls for IHC > > > > Hi Tanya, > > > > We have made the decision to continue running negative reagent control slides. > > > > Linda A. Sebree > > University of Wisconsin Hospital & Clinics > > IHC/ISH Laboratory > > 600 Highland Ave. > > Madison, WI 53792 > > (608)265-6596 > > FAX: (608)262-7174 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya > > Sent: Tuesday, February 11, 2014 12:54 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Negative Controls for IHC > > > > I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > > email: tanyaabbott@catholichealth.net > > > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > From anna.c.hughes <@t> gsk.com Wed Feb 12 10:14:20 2014 From: anna.c.hughes <@t> gsk.com (Anna Hughes) Date: Wed Feb 12 10:14:33 2014 Subject: [Histonet] Mouse F4/80 antibody Message-ID: <56B8D7633148A9419B559EBE8E9CF5900D8F9BB8@019-SN1MPN1-032.019D.MGD.MSFT.NET> Hi Everyone! I was wondering if any of you have a favorite CD68 macrophage antibody that you have used on mouse tissue and is especially reliable. This is a target that has been notorious in our lab and I was wondering if there was a really good one to use. Thanks in advance for your help! Anna Hughes Anna C. Hughes, BBA, BS, HTLCM anna.c.hughes@gsk.com From mward <@t> wakehealth.edu Wed Feb 12 10:23:01 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Wed Feb 12 10:23:09 2014 Subject: [Histonet] RE: Negative Controls for IHC In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF2D7FC772@CHIEX005.CHI.catholichealth.net>, , <77DD817201982748BC67D7960F2F76AF099575@UWHC-MBX12.uwhis.hosp.wisc.edu>, , Message-ID: We discontinued them over a year ago. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, February 12, 2014 11:04 AM To: Cheri Miller Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Negative Controls for IHC Cheryl Yes, I understand. It is highly likely this will be the way things go here also. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: cmiller@physlab.com > To: joelleweaver@hotmail.com > CC: histonet-bounces@lists.utsouthwestern.edu; > histonet@lists.utsouthwestern.edu > Date: Wed, 12 Feb 2014 08:04:43 -0600 > Subject: RE: [Histonet] RE: Negative Controls for IHC > > I just stopped using the reagent controls on all our IHC due to the drastic cuts in reimbursement. It will be a significant immediate savings for our tiny lab. > Cheryl A. Miller HT ASCP cm > Histology Supervisor > Hygiene Officer > Physicians Laboratory, P.C. > 4840 F St. > Omaha , NE. 68117 > 402 731 4145 ext. 532 > Cell 402 493 0403 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > [joelleweaver@hotmail.com] > Sent: Tuesday, February 11, 2014 1:54 PM > To: Sebree Linda A; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Negative Controls for IHC > > Not too long ago, there was a posting about labs that had discontinued. You technically don't need with a polymer, non-biotin system. I still do them- for now. The pathologist never uses them, internal controls only. But I do, but this may be a good cost cutting idea for the future. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: LSebree@uwhealth.org > > To: TanyaAbbott@catholichealth.net; > > histonet@lists.utsouthwestern.edu > > Date: Tue, 11 Feb 2014 19:44:37 +0000 > > CC: > > Subject: [Histonet] RE: Negative Controls for IHC > > > > Hi Tanya, > > > > We have made the decision to continue running negative reagent control slides. > > > > Linda A. Sebree > > University of Wisconsin Hospital & Clinics IHC/ISH Laboratory > > 600 Highland Ave. > > Madison, WI 53792 > > (608)265-6596 > > FAX: (608)262-7174 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > Abbott, Tanya > > Sent: Tuesday, February 11, 2014 12:54 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Negative Controls for IHC > > > > I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology St. Joseph Medical Center > > Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > > email: tanyaabbott@catholichealth.net > > > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi <@t> nrh-ok.com Wed Feb 12 11:06:38 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Wed Feb 12 11:06:41 2014 Subject: [Histonet] Leica Cryostat CM 1860 UV Decontamination Procedure Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B822006@MAIL.nrhnt.nrh-ok.com> Hi, How are you guys doing? Please I am wondering if some of you guys are using this type of Leica cryostat and would be interested in the decontamination procedure that you have in place. Thanks, Adesupo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From turkekul <@t> gmail.com Wed Feb 12 12:10:28 2014 From: turkekul <@t> gmail.com (Mesru T) Date: Wed Feb 12 12:10:37 2014 Subject: [Histonet] CD68 or F4/80 Message-ID: Hi Anna, Your subject line is F4/80 and you are asking for CD68. I thought they are different antibodies, but I may be wrong. We have never been able to find CD68 ab that works well of FFPE mouse tissues. Recently we had success with F4/80 Rat anti-mouse Clone: BM8 from BioLegend. It only worked with citric buffer pH6 100C 10min. We also use Iba-1 as macrophage marker on mouse FFPE tissues. If anyone has good CD68 on FFPE mouse tissues please could you share your experiences? It will be of great help. Hope this helps. Mesru From Nancy_Schmitt <@t> pa-ucl.com Wed Feb 12 12:19:27 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Feb 12 12:19:34 2014 Subject: [Histonet] negative controls for IHC In-Reply-To: <20140212180540.E95F41AA05D@mail.pa-ucl.com> References: <20140212180540.E95F41AA05D@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3681561B9F@PEITHA.wad.pa-ucl.com> We also stopped running negative controls more than a year ago. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA ================================================ From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, February 11, 2014 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls for IHC I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.ne NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From tbraud <@t> holyredeemer.com Wed Feb 12 12:40:08 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Feb 12 12:40:12 2014 Subject: [Histonet] Neg Ctls, Cryostat Decon, Tricrome stain and more In-Reply-To: <20140212172546.7BB421E805A@trendmess-svr.holyredeemer.local> References: <20140212172546.7BB421E805A@trendmess-svr.holyredeemer.local> Message-ID: Wow! Three things! Negative IHC Controls - Since we use a polymer detection system, we long ago quit using negative controls as a cost savings measure. I can't imagine doing anything less considering the cost. It has saved us thousands. UV cryostat decontamination - Unfortunately, if you are a CAP accredited lab, they do not recognize this as appropriate decontamination, but insist on a room temperature decontamination, (ANP.12087 "all exposed surfaces must be wiped with an EPA approved tuberculocidal disinfectant") We use these really handy pop up disinfectant wipes that are alcohol based, taking down one instrument for 48 hours to completely dry at room temp before wiping and restarting. I was told that CAP is looking into altering this based on differing disinfection systems, such as UV, but until then, there is no alternative if you are CAP Trichrome stain - Just switch to the Gomori's one step with Aniline Blue. Much easier, fewer steps, almost goof proof. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 ************************************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From amber.mckenzie <@t> gastrodocs.net Wed Feb 12 12:50:15 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Feb 12 12:50:33 2014 Subject: [Histonet] Ventana Vantage and H&E stainer In-Reply-To: References: <20140212172546.7BB421E805A@trendmess-svr.holyredeemer.local> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0112463868@JERRY.Gia.com> So, Ventana came out to my lab to do a LEAN consult for us since we are building a new lab...they want to sell us their vantage tracking system and H&E stainer. Our Rep is coming on Fri to talk to me, our lab director and CEO about these products. Anyone using these? I'd like some feedback so I can be prepared with questions on Fri for our meeting. Thanks! From BDeBrosse-Serra <@t> isisph.com Wed Feb 12 13:02:42 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Wed Feb 12 13:02:55 2014 Subject: [Histonet] CD68 or F4/80 In-Reply-To: References: Message-ID: We had success with CD68 on mouse tissue from Boster cat# PA1518. For F4/80 we use the rat anti-mouse antibody from eBioscience cat# 14-4801. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mesru T Sent: Wednesday, February 12, 2014 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD68 or F4/80 Hi Anna, Your subject line is F4/80 and you are asking for CD68. I thought they are different antibodies, but I may be wrong. We have never been able to find CD68 ab that works well of FFPE mouse tissues. Recently we had success with F4/80 Rat anti-mouse Clone: BM8 from BioLegend. It only worked with citric buffer pH6 100C 10min. We also use Iba-1 as macrophage marker on mouse FFPE tissues. If anyone has good CD68 on FFPE mouse tissues please could you share your experiences? It will be of great help. Hope this helps. Mesru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rebecca.Riesen <@t> hma.com Wed Feb 12 13:25:23 2014 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Wed Feb 12 13:25:20 2014 Subject: [Histonet] RE: Histonet Digest, Vol 123, Issue 12 In-Reply-To: <19740412024625.6F0C173184112E37@mx2.hma.com> References: <19740412024625.6F0C173184112E37@mx2.hma.com> Message-ID: I agree with most of what others have said concerning your question Anna, but I have another BIG issue that no one has mentioned: "Heating Bouin's in the Microwave". This practice is very unsafe! Bouin's (picric acid) is highly explosive and should NEVER be microwaved! Granted this is my opinion, but I was really frightened upon reading about this practice. Just want everyone to be safe. Message: 7 Date: Tue, 11 Feb 2014 14:44:05 -0500 From: Anna Coffey Subject: [Histonet] Troubleshooting Masson Trichrome Stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello Histonetters, We regularly perform the Masson Trichrome stain in our lab and recently have been noticing some inconsistent results. The main issue seems to be that the Biebrich Scarlet is not being adequately removed by the PTA/PMA, as many of the areas containing collagen are showing up as purple instead of bright blue. We use fresh reagents every time we run the stain and our protocols are as follows: Rehydrate (xylene --> 100%EtOH --> 95% --> 80% --> 70%) Bouin's in microwave (1 min) Stand at RT in Bouin's (15 min) Running tap (5 min) Weigert's Hx (10 min) Running Tap (5 min) Biebrich Scarlet (1.25 min) Rinse dH2O Phosphotungstic acid/Phosphomolybdic acid (10 min) Aniline Blue (10 min) Rinse dH2O 1% Acetic acid (1 min) Rinse dH2O Air dry for dehydration Has anyone else experienced this same problem and, if so, what corrective actions have worked for you? I'd greatly appreciate your advice! Thanks, Anna -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu From mucram11 <@t> comcast.net Wed Feb 12 13:37:41 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Feb 12 13:37:57 2014 Subject: [Histonet] Arkansas Society of Histotechnology Spring Meeting in Hot Springs In-Reply-To: <245096819.55284.1392233701322.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <500762639.55354.1392233861778.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We are only 2 weeks from the ASH Annual Spring Meeting in Hot Springs AR.? We are excited and ready to welcome everyone to our 2 day meeting for Histology CEUs and Roaring Twenties Meet and Greet.? ? Registration will open through both days on-site and anyone wishing to pre-register please contact me at this e-mail or ashnews@comcast.net for all of the programming and abstracts as well as registration forms and menus. ? We have the opportunity to allow you to choose which topics would interest you most or that you feel you would like to learn more about.? Please let me know if you need anything to help you decide to join us on February 28th and March 1st.? The choice of speakers and topics are exciting and we hope well rounded; as well as all being CEU approved by NSH for those who need credits.? The full offering if you choose to attend both days is 12 CEUs or 6 CEUs for one day.? We know those CEUs are needed and hope this helps everyone meet their goals for keeping update with ASCP and NSH. ? Shane Jones will be doing his "Do I Really Have To Know All of That For The Registry?" preparation course for taking the ASCP registry.? This is a full day class.? If you are getting ready to take the test and need a good preparation course in the area within your driving or other transportation mode joint us for the day.? We are providing lunch and breaks for all attendees.? ? Hope to see you all on February 28th and March 1st in Hot Springs, AR at the Austin Hotel and Spa for the meeting and fun!! ? Pamela Marcum ? ? From pruegg <@t> ihctech.net Wed Feb 12 14:02:07 2014 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Wed Feb 12 14:02:11 2014 Subject: [Histonet] RE: Mouse F4/80 antibody In-Reply-To: <56B8D7633148A9419B559EBE8E9CF5900D8F9BB8@019-SN1MPN1-032.019D.MGD.MSFT.NET> References: <56B8D7633148A9419B559EBE8E9CF5900D8F9BB8@019-SN1MPN1-032.019D.MGD.MSFT.NET> Message-ID: <003e01cf282d$4f4efe10$edecfa30$@ihctech.net> Rat anti ms F4/80 is used on mouse tissue ffpe for macrophages not anti human cd68. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Hughes Sent: Wednesday, February 12, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse F4/80 antibody Hi Everyone! I was wondering if any of you have a favorite CD68 macrophage antibody that you have used on mouse tissue and is especially reliable. This is a target that has been notorious in our lab and I was wondering if there was a really good one to use. Thanks in advance for your help! Anna Hughes Anna C. Hughes, BBA, BS, HTLCM anna.c.hughes@gsk.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kristopher.Kalleberg <@t> unilever.com Wed Feb 12 14:11:47 2014 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Wed Feb 12 14:12:28 2014 Subject: [Histonet] procollagen 1 Message-ID: Hello All, I have been using a procollagen antibody from Millipore and the latest lots now do not work. I am in the middle of running a clinical study which has a deadline that is closing in and would greatly appreciate if anyone could recommend a good procollagen antibody for skin. This would greatly aid in my validating and optimizing processes. Thank you in advance. Kris From GKeyser <@t> uwhealth.org Wed Feb 12 14:16:36 2014 From: GKeyser <@t> uwhealth.org (Keyser Gerald T) Date: Wed Feb 12 14:16:41 2014 Subject: [Histonet] RE: Leica Cryostat CM 1860 UV Decontamination Procedure In-Reply-To: <04EE4F75BB5FB246ADB68D69B74604438E3B822006@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B74604438E3B822006@MAIL.nrhnt.nrh-ok.com> Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE025380@UWHC-MBX12.uwhis.hosp.wisc.edu> We use a Leica CM1850 currently. May differer a bit. On a daily basis, we clean the loose trimmings and wipe the inside with 95% ethanol. The exterior surfaces are cleaned daily with caviwipes. Once a month we turn off the machine, remove the microtome (I don't know if this is proper for the CM1860), let it completely defrost. Then clean the entire chamber with 95% ethanol. The microtome is dyed completely and the cryostat reassembled. This procedure was in place long before I started working where I do. I'm not sure if this procedure is something that is leica approved. I believe the CM1860 has a different microtome construction than the CM1850. I'm also hoping that the next cryostats we get have UVC. I really like the idea of UVC. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Adesupo, Adesuyi (Banjo) [abadesuyi@nrh-ok.com] Sent: Wednesday, February 12, 2014 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Cryostat CM 1860 UV Decontamination Procedure Hi, How are you guys doing? Please I am wondering if some of you guys are using this type of Leica cryostat and would be interested in the decontamination procedure that you have in place. Thanks, Adesupo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amurvosh <@t> advancederm.net Wed Feb 12 14:18:29 2014 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Wed Feb 12 14:18:41 2014 Subject: [Histonet] Ventana Vantage and H&E stainer In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC0112463868@JERRY.Gia.com> References: <20140212172546.7BB421E805A@trendmess-svr.holyredeemer.local> <5A33C952BB67F4468AF1F36D739212BC0112463868@JERRY.Gia.com> Message-ID: <4AD6A4E531E8C943A730559B6B81DF07CE9EBE@dc.Advancederm.net> For the most part I like this system. A few things though. Their H&E racks constantly break so make sure in your contract you get free ones to replace them. Also if you have to remove a coverslip it is a pain. You have to heat the slide for a while so see if they will include a heater and you will need it. Every once and a while the coverslips get a bad lot and you get bubbles. Also the color of your cassettes can make a big difference on if the barcode reader can pick up the code so ask them about it. I don't work there any more so maybe they have fixed things Sacred Heart Hospital in Spokane Wa Has the complete system so you could give them a call. 509-474-4108 Thanks Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, February 12, 2014 10:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Vantage and H&E stainer So, Ventana came out to my lab to do a LEAN consult for us since we are building a new lab...they want to sell us their vantage tracking system and H&E stainer. Our Rep is coming on Fri to talk to me, our lab director and CEO about these products. Anyone using these? I'd like some feedback so I can be prepared with questions on Fri for our meeting. Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Feb 12 14:20:45 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Feb 12 14:20:52 2014 Subject: [Histonet] RE: procollagen 1 In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05409@SBS2K8.premierlab.local> Kris We have had good success with Procollagen Type I from Abcam ab64409 on human skin samples. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Wednesday, February 12, 2014 1:12 PM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] procollagen 1 Hello All, I have been using a procollagen antibody from Millipore and the latest lots now do not work. I am in the middle of running a clinical study which has a deadline that is closing in and would greatly appreciate if anyone could recommend a good procollagen antibody for skin. This would greatly aid in my validating and optimizing processes. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rebecca.Riesen <@t> hma.com Wed Feb 12 14:53:33 2014 From: Rebecca.Riesen <@t> hma.com (Riesen, Rebecca) Date: Wed Feb 12 14:53:27 2014 Subject: [Histonet] Bouin's in microwave Message-ID: Seems I've mis-spoken on this one. I realize that Bouin's is only explosive when dried, but drying could occur during the process of microwaving. Also, we use Saturated picric acid for our mordant rather than Bouin's to also not have the exposure to the formalin fumes associated with another Bouin's Solution ingredient. Yet another reason not to microwave "Bouin's". Hopefully you have Great ventilation for your microwave if you choose to engage in this practice. Rebecca S. Riesen, HT, ASCP Histology Supervisor PRHS Naples, FL 239-348-4327 rebecca.riesen@hma.com From koellingr <@t> comcast.net Wed Feb 12 15:50:52 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Feb 12 15:51:08 2014 Subject: [Histonet] RE: Mouse F4/80 antibody In-Reply-To: <003e01cf282d$4f4efe10$edecfa30$@ihctech.net> References: <56B8D7633148A9419B559EBE8E9CF5900D8F9BB8@019-SN1MPN1-032.019D.MGD.MSFT.NET> <003e01cf282d$4f4efe10$edecfa30$@ihctech.net> Message-ID: <320744416.2860714.1392241852041.JavaMail.root@comcast.net> Hello, I agree anti-human CD68 might not be best for mouse tissue but there are specific mouse CD68 (the?mouse homolog to human CD68) antibodies?out there.? FA-11 is one of my favorites.? Have used both F4/80 and (rat anti-mouse CD68) on flow, frozens and FFPE.? Anna, a literature search gives you published ?protocols.? I'm looking right now at a photo of some CD68+ macs staining?on mouse tissues I took from years back.? Shows them nicely.? anti-mouse CD68 on mouse tissue is in the literature. ? Ray Seattle ----- Original Message ----- From: pruegg@ihctech.net To: "Anna Hughes" , histonet@lists.utsouthwestern.edu Sent: Wednesday, February 12, 2014 12:02:07 PM Subject: [Histonet] RE: Mouse F4/80 antibody Rat anti ms F4/80 is used on mouse tissue ffpe for macrophages not anti human cd68. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Hughes Sent: Wednesday, February 12, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse F4/80 antibody Hi Everyone! I was wondering if any of you have a favorite CD68 macrophage antibody that you have used on mouse tissue and is especially reliable. ?This is a target that has been notorious in our lab and I was wondering if there was a really good one to use. Thanks in advance for your help! Anna Hughes Anna C. Hughes, BBA, BS, HTLCM anna.c.hughes@gsk.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Feb 12 15:58:50 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Feb 12 15:58:54 2014 Subject: [Histonet] RE: Mouse F4/80 antibody In-Reply-To: <320744416.2860714.1392241852041.JavaMail.root@comcast.net> References: <56B8D7633148A9419B559EBE8E9CF5900D8F9BB8@019-SN1MPN1-032.019D.MGD.MSFT.NET> <003e01cf282d$4f4efe10$edecfa30$@ihctech.net> <320744416.2860714.1392241852041.JavaMail.root@comcast.net> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05414@SBS2K8.premierlab.local> I'll chime in here and state that we have used F4/80, iba-1, and CD68 for mouse macrophages, for CD68 we use the same clone as Ray is talking about we get that particular antibody from Serotec. All work on FFPE tissues in our hands. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Wednesday, February 12, 2014 2:51 PM To: pruegg@ihctech.net Cc: histonet@lists.utsouthwestern.edu; Anna Hughes Subject: Re: [Histonet] RE: Mouse F4/80 antibody Hello, I agree anti-human CD68 might not be best for mouse tissue but there are specific mouse CD68 (the?mouse homolog to human CD68) antibodies?out there.? FA-11 is one of my favorites.? Have used both F4/80 and (rat anti-mouse CD68) on flow, frozens and FFPE.? Anna, a literature search gives you published ?protocols.? I'm looking right now at a photo of some CD68+ macs staining?on mouse tissues I took from years back.? Shows them nicely.? anti-mouse CD68 on mouse tissue is in the literature. ? Ray Seattle ----- Original Message ----- From: pruegg@ihctech.net To: "Anna Hughes" , histonet@lists.utsouthwestern.edu Sent: Wednesday, February 12, 2014 12:02:07 PM Subject: [Histonet] RE: Mouse F4/80 antibody Rat anti ms F4/80 is used on mouse tissue ffpe for macrophages not anti human cd68. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anna Hughes Sent: Wednesday, February 12, 2014 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse F4/80 antibody Hi Everyone! I was wondering if any of you have a favorite CD68 macrophage antibody that you have used on mouse tissue and is especially reliable. ?This is a target that has been notorious in our lab and I was wondering if there was a really good one to use. Thanks in advance for your help! Anna Hughes Anna C. Hughes, BBA, BS, HTLCM anna.c.hughes@gsk.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sarahholt_ht <@t> yahoo.com Wed Feb 12 19:12:43 2014 From: sarahholt_ht <@t> yahoo.com (Sarah Holt) Date: Wed Feb 12 19:13:16 2014 Subject: [Histonet] Good evening Histonet Message-ID: <20140213011314.DLYH7687.fed1rmfepo201.cox.net@fed1rmimpo110> http://izmirpeyzaj.gen.tr/res/twit.php?bktkkae1280eeku sarahholt_ht@yahoo.com Thu, 13 Feb 2014 02:12:43 From barryrittman <@t> gmail.com Thu Feb 13 07:20:00 2014 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Thu Feb 13 07:20:04 2014 Subject: [Histonet] Bouin's in microwave In-Reply-To: References: Message-ID: Rebecca I want to thank you for pointing out some of the dangers of picric acid and also of formalin fumes. One of the major problems we have in labs today is that although we have product sheets for chemicals most people do not read them and assume that if a material is in common use it is probably safe. I think that many technicians are not instructed on potential dangers of the chemicals they use. I was trained to regard all chemicals as potentially dangerous and to find out about the chemicals I would use before I started to use them. This may seem neurotic to some people but it stood me in good stead. A point to remember is that the chemical or process that is stated as being safe today may be shown in the future to be hazardous. For many chemicals the period of time it has been in use is small and long term effects unknown. I remember in Iowa when our house was insulated with expanded foam in the walls. At that time the process was said to be safe, however later the use of formaldehyde to set the plastic was deemed to be hazardous. One good unexpected consequence for us was that it euthanized in place a large nest of carpenter ants that had resisted all normal attempts at eradication. Rebecca thank you once again, keep safe. Barry On Wed, Feb 12, 2014 at 2:53 PM, Riesen, Rebecca wrote: > Seems I've mis-spoken on this one. I realize that Bouin's is only > explosive when dried, but drying could occur during the process of > microwaving. Also, we use Saturated picric acid for our mordant rather > than Bouin's to also not have the exposure to the formalin fumes associated > with another Bouin's Solution ingredient. Yet another reason not to > microwave "Bouin's". Hopefully you have Great ventilation for your > microwave if you choose to engage in this practice. > > Rebecca S. Riesen, HT, ASCP > Histology Supervisor > PRHS Naples, FL > 239-348-4327 > rebecca.riesen@hma.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From blayjorge <@t> gmail.com Thu Feb 13 10:31:29 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Thu Feb 13 10:31:33 2014 Subject: [Histonet] Microwave in histological/histochemical protocols Message-ID: Hello Histo-Listers: Could I have a sense of how common is the use of microwaving in histological/histochemical protocols? Are there standard protocols using microwave? Please, feel free to email me directly. Thank you. Jorge (blayjorge@gmail.com) Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From jcox90 <@t> yahoo.com Thu Feb 13 11:23:20 2014 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Thu Feb 13 11:23:23 2014 Subject: [Histonet] Histologist needed for Derm Lab Monday's and on-call for vacations/SL in Peoria AZ Message-ID: <1392312200.55973.YahooMailBasic@web160703.mail.bf1.yahoo.com> Hi Netters, We are looking for a Part-time Histologist for Monday coverage at a new Dermatology Lab in Peoria AZ. Will consist of embedding, cutting and grossing. Also looking for vacation and sick leave coverage. Wonderful practice to work for! Hour's can be flexible as we don't have Dr reading that day. Please email with questions then we can go from there. Thank you, Jill Arrowhead Dermatology From mroark <@t> sfmc.net Thu Feb 13 12:02:29 2014 From: mroark <@t> sfmc.net (Matthew D. Roark) Date: Thu Feb 13 12:03:02 2014 Subject: [Histonet] Glassware Cleaning Message-ID: <18f0eeb309bd471d927940a18cae5387@BLUPR04MB199.namprd04.prod.outlook.com> What is everyone using to test their glassware for detergent residue? Is there a ready to use solution or not? CAP Checklist... Glassware Cleaning Phase II There are appropriate documented procedures for handling and cleaning glassware, including methods for testing for detergent removal. NOTE: Special instructions for micropipettes, cuvets, acid washing, etc. must be included. A simple procedure to check for detergent residue uses bromcresol purple (0.1 g bromcresol purple in 50 mL ethyl alcohol). Pipette approximately 5 cm (2 inches) distilled water into a representative, washed, glassware item. Add two to three drops bromcresol solution. A purple color reveals residual detergent. A yellow color indicates satisfactory rinsing. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net From gayle.callis <@t> bresnan.net Thu Feb 13 12:03:36 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Feb 13 12:03:52 2014 Subject: [Histonet] Caveats Re: Bouins in microwave for Massons trichrome Message-ID: <000001cf28e5$ec98c350$c5ca49f0$@bresnan.net> Dear Histonetters, Microwaving Bouins can be an absolute disaster as experienced by me. It went all over the interior of microwave (borrowed one at that!) and created an unbelievable mess of picric acid that then dried out, and formaldehyde going into every nook and cranny of the microwave interior along with terrible fumes. I spent a good deal of time All I can say is DON"T MICROWAVE BOUINS!! Other caveats: #1 is already mentioned #2 Microwaving sections in Bouins can lead to spurious staining results. Too short a time can create poor staining with this protocol. As stated by Liz Chlipala, use a heated oven or waterbath at 56?C - 60?C for one hour to ensure the section is properly exposed Bouins acid components (picric and acetic acids) is a must for staining the connective tissue fibers. This is the classic way of doing Massons Trichrome and still works the best. A microwave must be properly vented in a hood, or it is a toxic mess of fumes, let alone having to clean up nasty picric acid that will dry out in crevices/corners of MW. You can use a one zip lock baggie, left open at one end of top to vent fumes, and collect any boil over, but staining problems have to considered due to short exposure to Bouins. This is something I learned from Jerry Fredenburgh many years ago, and after bulk staining several hundred decalcified bone sections, the classic method, correct time in Bouins gave the best and most consistent results. #3 If you are doing Mass Tri the next day, deparaffinize the sections, rehydrate and immerse in Room Temperature Bouins and let sections sit overnight. No heating means: no toxic formalin fumes from either MW or heated oven, problem and decalcified bone sections stay on slide as these can dislodge due to mechanical/physical forces from MW heating and sometimes with heating in an oven, and is safer for user. This became our favorite method, and no more spillage/toxic fume problems. If anyone wants an excellent Massons Trichrome method, the classic one I have from AFIP lab is superb. It also gives hints on achieving best results with proper differentiation of connective tissue fibers - something we often do not think about when doing this stain. Take care Gayle M. Callis HTL/HT/MT(ASCP) From lcolbert <@t> pathmdlabs.com Thu Feb 13 12:14:25 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Thu Feb 13 12:16:27 2014 Subject: [Histonet] Herpes I and II Message-ID: <12ECD7346266D74691EC2BFC75285E452F3FA597@BFL323E10.pathmdlabs.local> Can anyone tell me where I can purchase the Herpes I and II antibody combined and also IHC control slides for both type I and II - on one slide. Vendors welcome to respond. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From Erin.Martin <@t> ucsf.edu Thu Feb 13 12:20:59 2014 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Feb 13 12:21:15 2014 Subject: [Histonet] Negative Controls Message-ID: <24B7B291CC88D04AB663958E77A1F59D199BCD@ex09.net.ucsf.edu> Hi All, We are a Joint Commission inspected lab but after CAP changed their requirements for negative controls we were hoping to drop them too. My manager contacted TJC and asked what their position was regarding negative controls. They responded that they have not changed their requirements and therefore still want to see negative controls. Has anyone by inspected recently by TJC and had this issue come up? Thank you! Erin Martin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From wbenton <@t> cua.md Thu Feb 13 12:40:29 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Thu Feb 13 12:41:47 2014 Subject: [Histonet] RE: Glassware Cleaning In-Reply-To: <18f0eeb309bd471d927940a18cae5387@BLUPR04MB199.namprd04.prod.outlook.com> References: <18f0eeb309bd471d927940a18cae5387@BLUPR04MB199.namprd04.prod.outlook.com> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF40F@CUAEXH1.GCU-MD.local> Fisher sells a RTU solution of the bromcresol purple. We use that for our glassware. It works very well. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew D. Roark [mroark@sfmc.net] Sent: Thursday, February 13, 2014 1:02 PM To: Histonet@Lists. Edu Subject: [Histonet] Glassware Cleaning What is everyone using to test their glassware for detergent residue? Is there a ready to use solution or not? CAP Checklist... Glassware Cleaning Phase II There are appropriate documented procedures for handling and cleaning glassware, including methods for testing for detergent removal. NOTE: Special instructions for micropipettes, cuvets, acid washing, etc. must be included. A simple procedure to check for detergent residue uses bromcresol purple (0.1 g bromcresol purple in 50 mL ethyl alcohol). Pipette approximately 5 cm (2 inches) distilled water into a representative, washed, glassware item. Add two to three drops bromcresol solution. A purple color reveals residual detergent. A yellow color indicates satisfactory rinsing. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From TJohnson <@t> gnf.org Thu Feb 13 12:43:49 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Thu Feb 13 12:43:54 2014 Subject: [Histonet] Re: Bouins in microwave Message-ID: <9F3CFEE76E51B64991C7485270890B4049830A60@EX5.lj.gnf.org> Thank you Barry for your post. And good for you Rebecca in reflecting on your post and coming back with formalin fumes as being probably the leading danger of heating Bouins. Before there were microwaves used in the lab, we used to mordant at 60 degrees C for an hour in the oven. Opening the regular oven to remove the slides sometimes gave us a good dose of formalin fumes because the ovens were never vented nor placed in the fume hood! Several ways around this: -Picric acid mordant as Rebecca does -Room temperature Bouins overnight in the fume hood -Put your coplin jar/slide holder in a plastic ziplock bag prior to heating. After heat mordant, take the bag to the fume hood and open it. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From avistarop <@t> ffyb.uba.ar Thu Feb 13 12:45:50 2014 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Thu Feb 13 12:46:09 2014 Subject: [Histonet] Microwave in histological/histochemical protocols In-Reply-To: References: Message-ID: <0786801120bd179bff77575f0cec05b9.squirrel@huemul.ffyb.uba.ar> Hi Jorge, I use controled microwave or autoclave. I really used autoclave, is same, here attached the protocol that I use. I hope that this help you. Aldana > Hello Histo-Listers: > > Could I have a sense of how common is the use of microwaving in > histological/histochemical protocols? Are there standard protocols using > microwave? Please, feel free to email me directly. Thank you. > > Jorge (blayjorge@gmail.com) > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > http://blayjorge.wordpress.com/ > http://paleobiology.si.edu/staff/individuals/santiagoblay.html > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From avistarop <@t> ffyb.uba.ar Thu Feb 13 13:14:54 2014 From: avistarop <@t> ffyb.uba.ar (avistarop@ffyb.uba.ar) Date: Thu Feb 13 13:15:03 2014 Subject: [Histonet] IHC by low concentration nuclear protein Message-ID: Hello everyone!!!! One question: I?m doing a IHC (inmunehistochemical) by detection a nuclear protein that was founding in low concentration, but I don?t see nothing, I probed 7 protocols but I continue without see marque. Do you have any technical by IHC that can be useful by detection low concentration protein? Thak you Aldana Vistarop Laboratorio Biologi?a Molecular, Servicio de Anatomia Patologica, Hospital de Ni?os Ricardo Gutierrez. Ciudad de Buenos Aires Argentina From greyhoundallie <@t> yahoo.com Thu Feb 13 14:38:20 2014 From: greyhoundallie <@t> yahoo.com (Alison Romano) Date: Thu Feb 13 14:38:19 2014 Subject: [Histonet] IHC tutorial in California Message-ID: <000001cf28fb$890bada0$9b2308e0$@yahoo.com> Hello! A couple of months ago I received a flyer for an upcoming IHC tutorial for beginners in IHC. It is being held in San Francisco, in either March or April, 2014.(I think) I didn't save it because I didn't think I could go. Now, I can go, but don't know who to contact. Can anyone help? At the end of the flyer it said something to the effect of" You will be able to perform IHC in your lab after this class." Any help would be appreciated. Thank you, Alison From grantdebrar <@t> hotmail.com Thu Feb 13 14:42:09 2014 From: grantdebrar <@t> hotmail.com (Debra Grant) Date: Thu Feb 13 14:42:13 2014 Subject: [Histonet] Zinc Formalin Fixed H&E Stain Protocol for the Leica ST5020 Message-ID: Hi All, I am looking for a good H&E staining protocol to run on the Leica ST5020 for Zinc Formalin fixed tissues. Thank you in advanced! Deb Grant HT (QIHC) From BDeBrosse-Serra <@t> isisph.com Thu Feb 13 14:52:21 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Thu Feb 13 14:52:33 2014 Subject: [Histonet] IHC tutorial in California In-Reply-To: <000001cf28fb$890bada0$9b2308e0$@yahoo.com> References: <000001cf28fb$890bada0$9b2308e0$@yahoo.com> Message-ID: http://innvx.com/workshop.html Here you go! Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alison Romano Sent: Thursday, February 13, 2014 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC tutorial in California Hello! A couple of months ago I received a flyer for an upcoming IHC tutorial for beginners in IHC. It is being held in San Francisco, in either March or April, 2014.(I think) I didn't save it because I didn't think I could go. Now, I can go, but don't know who to contact. Can anyone help? At the end of the flyer it said something to the effect of" You will be able to perform IHC in your lab after this class." Any help would be appreciated. Thank you, Alison _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Thu Feb 13 16:38:06 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu Feb 13 16:38:27 2014 Subject: [Histonet] Microwave in histological/histochemical protocols In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00AFF60@xmdb04.nch.kids> Yep We use microwave for rapid processing of renal, cardiac and liver biopsies and Accelerated formalin fixation of tissues and Copper stain using rhodanine, Luxol fast blue for myelin Sulphated Alcian blue for amyloid Orcein for elastic fibres Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jorge A. Santiago-Blay Sent: Friday, 14 February 2014 3:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave in histological/histochemical protocols Hello Histo-Listers: Could I have a sense of how common is the use of microwaving in histological/histochemical protocols? Are there standard protocols using microwave? Please, feel free to email me directly. Thank you. Jorge (blayjorge@gmail.com) Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From BergerR1 <@t> email.chop.edu Fri Feb 14 10:07:20 2014 From: BergerR1 <@t> email.chop.edu (Berger, Rebecca) Date: Fri Feb 14 10:07:25 2014 Subject: [Histonet] Mouse Joints Message-ID: <5A590EB108038B4FA01F8CDB39C6541B01313552@EXCMBXPW7.chop.edu> Hey Histonet! I have to troubleshoot some mouse knee joints that were not completely decalcified before processing. I received them already embedded and they're almost impossible to cut. Any tips to get them decalcified? Thanks! Becky From galinadeyneko <@t> yahoo.com Fri Feb 14 10:06:13 2014 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Fri Feb 14 10:09:11 2014 Subject: [Histonet] Treatment of calcified human arteries Message-ID: <1392393973.74315.YahooMailNeo@web160201.mail.bf1.yahoo.com> Dear Colleagues ?Please share your expertise on?treating heavily calcified human arteries. It is impossible to cut them without deminiralization in my opinion. Should I use decalcification solution before processing? If yes, what reagent do you recommend taking in account that I will do IHC?for macrophages and other targets on these samples, as well as Sirius Red and Masson Trichrome staining. ?Thank you in advance Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w From galinadeyneko <@t> yahoo.com Fri Feb 14 10:06:13 2014 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Fri Feb 14 10:09:17 2014 Subject: [Histonet] Treatment of calcified human arteries Message-ID: <1392393973.74315.YahooMailNeo@web160201.mail.bf1.yahoo.com> Dear Colleagues ?Please share your expertise on?treating heavily calcified human arteries. It is impossible to cut them without deminiralization in my opinion. Should I use decalcification solution before processing? If yes, what reagent do you recommend taking in account that I will do IHC?for macrophages and other targets on these samples, as well as Sirius Red and Masson Trichrome staining. ?Thank you in advance Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w From liz <@t> premierlab.com Fri Feb 14 10:24:39 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Feb 14 10:24:47 2014 Subject: [Histonet] Treatment of calcified human arteries In-Reply-To: <1392393973.74315.YahooMailNeo@web160201.mail.bf1.yahoo.com> References: <1392393973.74315.YahooMailNeo@web160201.mail.bf1.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05425@SBS2K8.premierlab.local> Galina I would decal in 5-10% formic acid that should work just fine for IHC staining. You would fix, decal and then process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galina Deyneko Sent: Friday, February 14, 2014 9:06 AM To: histology; histonet@lists.utsouthwestern.edu Subject: [Histonet] Treatment of calcified human arteries Dear Colleagues ?Please share your expertise on?treating heavily calcified human arteries. It is impossible to cut them without deminiralization in my opinion. Should I use decalcification solution before processing? If yes, what reagent do you recommend taking in account that I will do IHC?for macrophages and other targets on these samples, as well as Sirius Red and Masson Trichrome staining. ?Thank you in advance Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Feb 14 10:27:36 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Feb 14 10:27:41 2014 Subject: [Histonet] RE: Mouse Joints In-Reply-To: <5A590EB108038B4FA01F8CDB39C6541B01313552@EXCMBXPW7.chop.edu> References: <5A590EB108038B4FA01F8CDB39C6541B01313552@EXCMBXPW7.chop.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05426@SBS2K8.premierlab.local> Becky We will do one of two things, we place the blocks in decal prior to placing on ice or we place some decal on top of the ice and then placed the trimmed block on the ice with the decal solution. If they are not decaled well at all it may be best just to reprocess the samples. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Berger, Rebecca Sent: Friday, February 14, 2014 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse Joints Hey Histonet! I have to troubleshoot some mouse knee joints that were not completely decalcified before processing. I received them already embedded and they're almost impossible to cut. Any tips to get them decalcified? Thanks! Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Fri Feb 14 10:41:06 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Feb 14 10:41:13 2014 Subject: [Histonet] RE: Negative Controls for IHC In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF2D7FC772@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF2D7FC772@CHIEX005.CHI.catholichealth.net> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0226A088@HHCEXCHMB03.hhcsystem.org> I apologize for the delay in responding to your e-mail; I just returned from the 2014 Applied IHC and Molecular Morphology meeting in Miami where this subject was discussed (as you might expect). The answer is, "Yes". The bottom line is that every laboratory running non-avidin-biotin-based diagnostic IHC tests must evaluate their own experience with "Negative Reagent Controls" (NRCs). If they prove useful to the pathologists reading the IHC slides, continue to run them. If not, discontinue them and conserve precious patient tissue/cells that can be used for additional testing, and, at the same time, save you laboratory thousands of dollars. I have an Editorial on this subject coming out in a major journal next month. I've been told that there is an article coming as well. I added "NRC" to our antibody dictionary years ago so that pathologists can order a NRC whenever they need it "after" reviewing their IHC slides. During a 5 year period, only "28" NRCs were ordered and I ordered "22" of them (primarily for education and teaching purposes). Keep in mind that my laboratory runs 40-50,000 IHC tests per year. Make you own decision .... Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, February 11, 2014 1:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls for IHC I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From gayle.callis <@t> bresnan.net Fri Feb 14 10:44:31 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Feb 14 10:44:38 2014 Subject: [Histonet] Treatment of calcified human arteries In-Reply-To: <1392393973.74315.YahooMailNeo@web160201.mail.bf1.yahoo.com> References: <1392393973.74315.YahooMailNeo@web160201.mail.bf1.yahoo.com> Message-ID: <000c01cf29a4$0a654ca0$1f2fe5e0$@bresnan.net> Dear Galina, Formic acid, buffered that contain either sodium citrate or sodium formate that have an approximate 4.5% formic acid concentration (yes, I did the calculation one time) and as made from 90% stock formic acid. You can buy these decalcifying solutions from a vendor, but look at the MSDS so you know what is in the solution. These are both well known e.g. "classic" formic acid solutions. If you are into making up your own solutions, what Liz Chliplala suggested is excellent although not buffered. Make sure your arteries are totally fixed in order to protect the antigens from effects of acid decalcification. I replied yesterday about Massons Trichrome and have a protocol with a modified, more concentrated Weigerts Iron Hematoxylin that proved to be far superior for decalcified tissues/bones. The nuclei were never differentiated out to the point of weak staining. I will be happy to send if you want it. Good luck Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galina Deyneko Sent: Friday, February 14, 2014 9:06 AM To: histology; histonet@lists.utsouthwestern.edu Subject: [Histonet] Treatment of calcified human arteries Dear Colleagues ?Please share your expertise on?treating heavily calcified human arteries. It is impossible to cut them without deminiralization in my opinion. Should I use decalcification solution before processing? If yes, what reagent do you recommend taking in account that I will do IHC?for macrophages and other targets on these samples, as well as Sirius Red and Masson Trichrome staining. ?Thank you in advance Galina Deyneko Novartis, Cambridge, MA ? 617-871-7613 w _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Feb 14 10:54:41 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Feb 14 10:54:48 2014 Subject: [Histonet] Mouse Joints In-Reply-To: <5A590EB108038B4FA01F8CDB39C6541B01313552@EXCMBXPW7.chop.edu> References: <5A590EB108038B4FA01F8CDB39C6541B01313552@EXCMBXPW7.chop.edu> Message-ID: <000d01cf29a5$76127ee0$62377ca0$@bresnan.net> Dear Becky, You didn't say what decalcifier you were using? It is a good idea to test the decalcification endpoint on these bones before processing . I have a very easy weight loss/weight gain method to make sure the calcium is removed from these joints or any bone for that matter. We used this for murine knees, all long bones and paws and never had problems with residual calcium. Two things: 1. Now that the bones are embedded and contain calcium, you can surface decalcify by immersing the trimmed block face into any acid decalcifying solution, particularly one from a vendor, for a few minutes. This will remove the calcium from only a few um of the sample, so be careful to NOT trim this minimally decalcified portion away when you resection the block. Be sure to rinse the acid off the block or metal parts on the microtome will corrode. You can re-cool the block after surface decalcification. This will take time for each block as you would have to repeat this each time you want to cut further into the sample. 2. If you have a tungsten carbide knife, you could try sectioning with the TC knife, and not surface decalcify. Good luck Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Berger, Rebecca Sent: Friday, February 14, 2014 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mouse Joints Hey Histonet! I have to troubleshoot some mouse knee joints that were not completely decalcified before processing. I received them already embedded and they're almost impossible to cut. Any tips to get them decalcified? Thanks! Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Fri Feb 14 12:31:01 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Feb 14 12:31:13 2014 Subject: [Histonet] RE: TJC on IHC Neg Controls In-Reply-To: <20140214172356.CB24C1E8056@trendmess-svr.holyredeemer.local> References: <20140214172356.CB24C1E8056@trendmess-svr.holyredeemer.local> Message-ID: I don't know who your manager talked to, but he/she has been misinformed. There is no such specific Joint Commission Standard. Here is what they state, copied from their standards: QCP.2.1.1 When immunohistochemistry is performed, the laboratory has appropriate quality control processes. Also, TJC defers to CAP regulations Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 4 Date: Thu, 13 Feb 2014 18:20:59 +0000 From: "Martin, Erin" Subject: [Histonet] Negative Controls Hi All, We are a Joint Commission inspected lab but after CAP changed their requirements for negative controls we were hoping to drop them too. My manager contacted TJC and asked what their position was regarding negative controls. They responded that they have not changed their requirements and therefore still want to see negative controls. Has anyone by inspected recently by TJC and had this issue come up? Thank you! Erin Martin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From BZIMMERM <@t> gru.edu Fri Feb 14 12:31:59 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Feb 14 12:32:05 2014 Subject: [Histonet] HISTOPALOOZA April 25 - 27, 2014 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF810636@EX-MLB-03.ad.georgiahealth.edu> I hope all of you wondered where I've been. I have survived the winter ice storm in Augusta, Georgia. Let's just say if Jim Cantore, from the Weather Channel, arrives in your city, you know it's the kiss of death! When I heard he had arrived, I knew we were doomed. But, I'm baaaack! Today's feature is a tag team, Jim and Theresa Burchette. Jim will be conducting a workshop titled "Immunohistochemical Detection of Infectious Diseases." The brochure says audience participation is encouraged. I have no idea what that could mean. It's either sharing of proper staining patterns or how to hand tie a tootie fruitie fly for fishing. Theresa's presentation is titled "Immunoreactivity in Normal Tissue". This is an extremely helpful workshop for selecting and maintaining IHC tissue controls. Both of these workshops will serve as great contact hours if you are wanting to maintain your QIHC or are interested in learning about IHC. Jim and Theresa are both wonderful speakers and I promise you won't have to play candy crush under the table to stay awake. From nbrabson <@t> MPLNet.com Fri Feb 14 13:21:33 2014 From: nbrabson <@t> MPLNet.com (Nani Brabson) Date: Fri Feb 14 13:22:40 2014 Subject: [Histonet] In need of Amyloid Control Tissue Message-ID: <908827E7E3D5094BA8495A3D40ADEFE00137DC387E7C@MAIL01.mplnet.com> Does anyone have any Amyloid tissue they are willing to share? We are a small lab and Amyloid is very hard for us to come by. Thanks, Nani Maryville, TN From Glenn.Hauck <@t> albertahealthservices.ca Fri Feb 14 14:18:18 2014 From: Glenn.Hauck <@t> albertahealthservices.ca (Glenn Hauck) Date: Fri Feb 14 14:18:29 2014 Subject: [Histonet] End time for fixation of breast tissue Message-ID: <38FA8FCA474F834CB9B90590D7C72390015A681C5C4B@EXMBX4C.crha.bewell.ca> Is there someone out there that can help me find out if there is any value in recording the end time for fixation of breast tissue in 10% formalin? We at present record the devitalization time, the time tissue is put into fixative and the time the tissue is cut into and exposed to 10% formalin as well as the time the processor is started Thanks Glenn Hauck Charge Tecnologist Pathology QueenElizabeth II Hospital Grande Prairie, AB T8V 2E8 780-538-7429 Work Main 780-538-7184 Work Office glenn.hauck@albertahealthservices.ca ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 14 14:40:14 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Feb 14 15:42:01 2014 Subject: [Histonet] RE: End time for fixation of breast tissue In-Reply-To: <38FA8FCA474F834CB9B90590D7C72390015A681C5C4B@EXMBX4C.crha.bewell.ca> References: <38FA8FCA474F834CB9B90590D7C72390015A681C5C4B@EXMBX4C.crha.bewell.ca> Message-ID: <761E2B5697F795489C8710BCC72141FF36758028@ex07.net.ucsf.edu> Glenn, You do need to show it was in formalin for at least 6 hours. we work backwards from the time on the processor that the tissue leaves the formalin. For us 3pm is the cutoff for breast tissue to get 6 hours fixation. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glenn Hauck Sent: Friday, February 14, 2014 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] End time for fixation of breast tissue Is there someone out there that can help me find out if there is any value in recording the end time for fixation of breast tissue in 10% formalin? We at present record the devitalization time, the time tissue is put into fixative and the time the tissue is cut into and exposed to 10% formalin as well as the time the processor is started Thanks Glenn Hauck Charge Tecnologist Pathology QueenElizabeth II Hospital Grande Prairie, AB T8V 2E8 780-538-7429 Work Main 780-538-7184 Work Office glenn.hauck@albertahealthservices.ca ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Fri Feb 14 16:33:22 2014 From: azdudley <@t> hotmail.com (anita) Date: Fri Feb 14 16:33:28 2014 Subject: [Histonet] ANP. 23045 performance of all instuments Message-ID: What are others saying in your procedures for this? Not sure just to put they should all be checked to see if running the way they should be? Thanks for the help, everyone have a great weekend!!! Anita Dudley Mobile, AL. From Yves.Heremans <@t> vub.ac.be Sat Feb 15 09:26:49 2014 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Sat Feb 15 09:26:57 2014 Subject: [Histonet] adverse effect of prolonged paraffin embedding on staining ? Message-ID: <28E38E81-985F-4357-AE65-F0E0369F73F1@vub.ac.be> Dear all, Is there any (published ?) negative effect when tissues stay in hot paraffin over the weekend on antibody staining (diminished staining intenstity ?) ? Yves From akemiat3377 <@t> yahoo.com Sat Feb 15 19:01:21 2014 From: akemiat3377 <@t> yahoo.com (Eileen Akemi Allison-Tacha) Date: Sat Feb 15 19:01:23 2014 Subject: [Histonet] Trade H-Pylori for Fungus Control Message-ID: <0AEE0F9A-6FCA-4C24-8FCC-6AA249DE5BCD@yahoo.com> Hi everyone in histo land! I hope you all had a wonderful Valentines Day. I am hoping I might get a Valentines gift of some fungus control tissue. I am getting down to the tail end of mine. I would be more than happy to trade some (+) H Pylori blocks in exchange. I would be more than happy to pay for the shipping charges. Thank you in advance, Akemi Allison, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory Email: aallison@montereygi.com Tele: (831) 375-3577 X117 From k.leigh.adams.865 <@t> gmail.com Sun Feb 16 12:36:25 2014 From: k.leigh.adams.865 <@t> gmail.com (Karen) Date: Sun Feb 16 12:36:33 2014 Subject: [Histonet] Re: Histonet Digest, Vol 123, Issue 15 In-Reply-To: <52ffab48.e72b3c0a.6830.1ba8SMTPIN_ADDED_MISSING@mx.google.com> References: <52ffab48.e72b3c0a.6830.1ba8SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <2D8C4855-D5A2-4410-A0CC-674D7C13D51B@gmail.com> Yves, You did not mention the melting point of your paraffin...any temperature above 60 degrees or prolonged exposure of heat may destroy protein in the tissue causing structurally damaged antigenic sites. This would invalidate your IHC results as staining would not be representative of what was originally present. Leigh Sent from k. Leigh's iPad. > On Feb 15, 2014, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: TJC on IHC Neg Controls (Terri Braud) > 2. HISTOPALOOZA April 25 - 27, 2014 (Zimmerman, Billie) > 3. In need of Amyloid Control Tissue (Nani Brabson) > 4. End time for fixation of breast tissue (Glenn Hauck) > 5. RE: End time for fixation of breast tissue (Morken, Timothy) > 6. ANP. 23045 performance of all instuments (anita) > 7. adverse effect of prolonged paraffin embedding on staining ? > (Yves Heremans) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 14 Feb 2014 13:31:01 -0500 > From: "Terri Braud" > Subject: [Histonet] RE: TJC on IHC Neg Controls > To: > Cc: Erin.Martin@ucsf.edu > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I don't know who your manager talked to, but he/she has been > misinformed. There is no such specific Joint Commission Standard. Here > is what they state, copied from their standards: > QCP.2.1.1 When immunohistochemistry is performed, the laboratory has > appropriate > quality control processes. > Also, TJC defers to CAP regulations > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > > Message: 4 > Date: Thu, 13 Feb 2014 18:20:59 +0000 > From: "Martin, Erin" > Subject: [Histonet] Negative Controls > > > Hi All, > We are a Joint Commission inspected lab but after CAP changed their > requirements for negative controls we were hoping to drop them too. My > manager contacted TJC and asked what their position was regarding > negative controls. They responded that they have not changed their > requirements and therefore still want to see negative controls. Has > anyone by inspected recently by TJC and had this issue come up? > Thank you! > Erin Martin > Erin Martin, Histology Supervisor > UCSF Dermatopathology Service > 415-353-7248 > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it was sent. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > > > ------------------------------ > > Message: 2 > Date: Fri, 14 Feb 2014 18:31:59 +0000 > From: "Zimmerman, Billie" > Subject: [Histonet] HISTOPALOOZA April 25 - 27, 2014 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <7B3DEB32E69C034EACB479059C5DE3FF810636@EX-MLB-03.ad.georgiahealth.edu> > > Content-Type: text/plain; charset="us-ascii" > > I hope all of you wondered where I've been. I have survived the winter ice storm in Augusta, Georgia. Let's just say if Jim Cantore, from the Weather Channel, arrives in your city, you know it's the kiss of death! When I heard he had arrived, I knew we were doomed. > But, I'm baaaack! Today's feature is a tag team, Jim and Theresa Burchette. Jim will be conducting a workshop titled "Immunohistochemical Detection of Infectious Diseases." The brochure says audience participation is encouraged. I have no idea what that could mean. It's either sharing of proper staining patterns or how to hand tie a tootie fruitie fly for fishing. Theresa's presentation is titled "Immunoreactivity in Normal Tissue". This is an extremely helpful workshop for selecting and maintaining IHC tissue controls. > Both of these workshops will serve as great contact hours if you are wanting to maintain your QIHC or are interested in learning about IHC. Jim and Theresa are both wonderful speakers and I promise you won't have to play candy crush under the table to stay awake. > > > > ------------------------------ > > Message: 3 > Date: Fri, 14 Feb 2014 14:21:33 -0500 > From: Nani Brabson > Subject: [Histonet] In need of Amyloid Control Tissue > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <908827E7E3D5094BA8495A3D40ADEFE00137DC387E7C@MAIL01.mplnet.com> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have any Amyloid tissue they are willing to share? We are a small lab and Amyloid is very hard for us to come by. > Thanks, > Nani > Maryville, TN > > > > > ------------------------------ > > Message: 4 > Date: Fri, 14 Feb 2014 13:18:18 -0700 > From: Glenn Hauck > Subject: [Histonet] End time for fixation of breast tissue > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <38FA8FCA474F834CB9B90590D7C72390015A681C5C4B@EXMBX4C.crha.bewell.ca> > Content-Type: text/plain; charset="us-ascii" > > Is there someone out there that can help me find out if there is any value in recording the end time for fixation of breast tissue in 10% formalin? > > We at present record the devitalization time, the time tissue is put into fixative and the time the tissue is cut into and exposed to 10% formalin as well as the time the processor is started > > Thanks > > > Glenn Hauck > Charge Tecnologist > Pathology > QueenElizabeth II Hospital > Grande Prairie, AB T8V 2E8 > > 780-538-7429 Work Main > 780-538-7184 Work Office > glenn.hauck@albertahealthservices.ca > > > > ________________________________ > This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. > > > ------------------------------ > > Message: 5 > Date: Fri, 14 Feb 2014 20:40:14 +0000 > From: "Morken, Timothy" > Subject: [Histonet] RE: End time for fixation of breast tissue > To: "'Glenn Hauck'" > Cc: Histonet > Message-ID: > <761E2B5697F795489C8710BCC72141FF36758028@ex07.net.ucsf.edu> > Content-Type: text/plain; charset=us-ascii > > Glenn, You do need to show it was in formalin for at least 6 hours. we work backwards from the time on the processor that the tissue leaves the formalin. For us 3pm is the cutoff for breast tissue to get 6 hours fixation. > > Tim Morken > Supervisor, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glenn Hauck > Sent: Friday, February 14, 2014 12:18 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] End time for fixation of breast tissue > > Is there someone out there that can help me find out if there is any value in recording the end time for fixation of breast tissue in 10% formalin? > > We at present record the devitalization time, the time tissue is put into fixative and the time the tissue is cut into and exposed to 10% formalin as well as the time the processor is started > > Thanks > > > Glenn Hauck > Charge Tecnologist > Pathology > QueenElizabeth II Hospital > Grande Prairie, AB T8V 2E8 > > 780-538-7429 Work Main > 780-538-7184 Work Office > glenn.hauck@albertahealthservices.ca > > > > ________________________________ > This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Fri, 14 Feb 2014 16:33:22 -0600 > From: anita > Subject: [Histonet] ANP. 23045 performance of all instuments > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > What are others saying in your procedures for this? Not sure just to put they should all be checked to see if running the way they should be? Thanks for the help, everyone have a great weekend!!! > > > > Anita Dudley > > Mobile, AL. > > > ------------------------------ > > Message: 7 > Date: Sat, 15 Feb 2014 16:26:49 +0100 > From: Yves Heremans > Subject: [Histonet] adverse effect of prolonged paraffin embedding on > staining ? > To: histonet@lists.utsouthwestern.edu > Message-ID: <28E38E81-985F-4357-AE65-F0E0369F73F1@vub.ac.be> > Content-Type: text/plain; charset=us-ascii > > Dear all, > > Is there any (published ?) negative effect when tissues stay in hot paraffin over the weekend on antibody staining (diminished staining intenstity ?) ? > > Yves > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 123, Issue 15 > ***************************************** From chesarato <@t> hotmail.com Sun Feb 16 20:44:45 2014 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Sun Feb 16 20:44:52 2014 Subject: [Histonet] Mineral Oil in Microwave Processing. Message-ID: Dear People from Histonet Do you still use Mineral Oil in your Microwave Processing Protocol ? Or you only use ethyl alcohol ~ Isopropyl alcohol and Paraffin . I will apreciate any advice. Thank you all Cesar Romero Buenos Aires Argentina From akemiat3377 <@t> yahoo.com Sun Feb 16 20:58:28 2014 From: akemiat3377 <@t> yahoo.com (Eileen Akemi Allison-Tacha) Date: Sun Feb 16 20:58:35 2014 Subject: [Histonet] Mineral Oil in Microwave Processing. In-Reply-To: References: Message-ID: I have a Milestone Microwave Tissue Processor and use Prowave from Milestone. It?s proprietary, but it has alcohol in it and does a great job on our tissue.I haven?t used mineral oil for years! Akemi Allison HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aallison@montereygi.com (831) 375-3577 X117 On Feb 16, 2014, at 6:44 PM, Cesar Francisco Romero wrote: > > > > Dear People from > Histonet > > > Do you still use > Mineral Oil in your Microwave Processing Protocol ? > > > Or you only use > ethyl alcohol ~ Isopropyl alcohol and Paraffin . > > > I will apreciate > any advice. > > > Thank you all > > > Cesar Romero > > > Buenos Aires > > > Argentina > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbell <@t> fmh.org Mon Feb 17 06:09:48 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Mon Feb 17 06:10:01 2014 Subject: [Histonet] Mineral Oil in Microwave Processing. In-Reply-To: References: Message-ID: <3566D9E34287BE4B95372179009446A01A47D4F6@EXCHANGE.fmhnt.fmh.org> I have never heard of using mineral oil in microwave processing. I only use ethyl and isopropyl and paraffin. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cesar Francisco Romero Sent: Sunday, February 16, 2014 9:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mineral Oil in Microwave Processing. Dear People from Histonet Do you still use Mineral Oil in your Microwave Processing Protocol ? Or you only use ethyl alcohol ~ Isopropyl alcohol and Paraffin . I will apreciate any advice. Thank you all Cesar Romero Buenos Aires Argentina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Feb 17 07:05:52 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Feb 17 07:05:56 2014 Subject: [Histonet] Mineral Oil in Microwave Processing. In-Reply-To: References: Message-ID: mineral oil was the worst to work with, and only partially counteracted the acetone. I as far as I am aware, it has been replaced in the later models. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: chesarato@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Sun, 16 Feb 2014 23:44:45 -0300 > Subject: [Histonet] Mineral Oil in Microwave Processing. > > > > > Dear People from > Histonet > > > Do you still use > Mineral Oil in your Microwave Processing Protocol ? > > > Or you only use > ethyl alcohol ~ Isopropyl alcohol and Paraffin . > > > I will apreciate > any advice. > > > Thank you all > > > Cesar Romero > > > Buenos Aires > > > Argentina > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Mon Feb 17 07:53:40 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Mon Feb 17 07:53:51 2014 Subject: [Histonet] RE: End time for fixation of breast tissue In-Reply-To: <761E2B5697F795489C8710BCC72141FF36758028@ex07.net.ucsf.edu> References: <38FA8FCA474F834CB9B90590D7C72390015A681C5C4B@EXMBX4C.crha.bewell.ca> <761E2B5697F795489C8710BCC72141FF36758028@ex07.net.ucsf.edu> Message-ID: We have the nurses put the time the breast is placed in formalin on the requisition as well as the time it is removed from the body. Cold ischemic time must be documented as well. This is important when the tissue goes for xray and is not immediately placed in formalin. Also, the limit for time in formalin is 72 hours, so you need to document - even if more than 72 hours. Happy Monday!! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 14, 2014 3:40 PM To: 'Glenn Hauck' Cc: Histonet Subject: [Histonet] RE: End time for fixation of breast tissue Glenn, You do need to show it was in formalin for at least 6 hours. we work backwards from the time on the processor that the tissue leaves the formalin. For us 3pm is the cutoff for breast tissue to get 6 hours fixation. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glenn Hauck Sent: Friday, February 14, 2014 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] End time for fixation of breast tissue Is there someone out there that can help me find out if there is any value in recording the end time for fixation of breast tissue in 10% formalin? We at present record the devitalization time, the time tissue is put into fixative and the time the tissue is cut into and exposed to 10% formalin as well as the time the processor is started Thanks Glenn Hauck Charge Tecnologist Pathology QueenElizabeth II Hospital Grande Prairie, AB T8V 2E8 780-538-7429 Work Main 780-538-7184 Work Office glenn.hauck@albertahealthservices.ca ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From thomasebrooks <@t> aol.com Mon Feb 17 08:30:10 2014 From: thomasebrooks <@t> aol.com (thomasebrooks) Date: Mon Feb 17 08:32:17 2014 Subject: [Histonet] Mineral Oil in Microwave Processing. Message-ID: There are at least two excellent papers that come to mind utilizing Mineral Oil: "Histology without Xylene" by Rene J. Buesa "Experience with an Automated Microwave-Assisted Rapid Tissue Processing Method" by Morales et Al Thomas Brooks ? Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: "Campbell, Tasha M." Date:02/17/2014 6:09 AM (GMT-06:00) To: Cesar Francisco Romero ,histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mineral Oil in Microwave Processing. I have never heard of using mineral oil in microwave processing.? I only use ethyl and isopropyl and paraffin. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cesar Francisco Romero Sent: Sunday, February 16, 2014 9:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mineral Oil in Microwave Processing. Dear People from Histonet Do you still use Mineral Oil in your Microwave Processing Protocol ? Or you only use ethyl alcohol ~ Isopropyl alcohol and Paraffin . I will apreciate any advice. Thank you all Cesar Romero Buenos Aires Argentina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marielachertoff <@t> gmail.com Mon Feb 17 08:47:26 2014 From: marielachertoff <@t> gmail.com (Mariela Chertoff) Date: Mon Feb 17 08:47:31 2014 Subject: [Histonet] waves in the surface of vibratome cuttings Message-ID: Hi averyone, We cut some mouse brains (postfixed 24hrs in PFA 4%), we cut at 40um and when we made Nissl staining we obseved waves of staining (more and less staining frames). We cut at 70Hz, amplitud 0.8mm and spead 0.025mm/sec. I believe that is a problem at the moment of cutting, but we don?t know how to solve this problem? Any advice? Thak you very much for your help!!! Mariela Chertoff, PhD Laboratorio de Biolog?a Molecular - QB75 Departamento de Qu?mica Biol?gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell?n II Piso 4 Ciudad de Buenos Aires - Argentina Tel: 54 11 4576-3300/09 - Int. 221 email: marielachertoff@qb.fcen.uba.ar marielachertoff@gmail.com From tbraud <@t> holyredeemer.com Mon Feb 17 08:59:03 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Feb 17 08:59:08 2014 Subject: [Histonet] Breast formalin time In-Reply-To: <20140215172213.5CE301E8060@trendmess-svr.holyredeemer.local> References: <20140215172213.5CE301E8060@trendmess-svr.holyredeemer.local> Message-ID: CAP and the American Society of Clinical Oncology (ASCO) provide guidelines for HER2 testing, and ER, PgR testing. The American College of Surgeons also require following CAP requirements for breast reporting for NAPBC (National Accreditation Program for Breast Centers) accreditation. As of Oct., 2013, those standards were: The time between breast tissue removal and the initiation of fixation should be documented to be less than or equal to one hour. Additionally, breast tissue resection specimens that are not immediately examined, such as those acquired in a location remote from the pathology laboratory, should be bisected through the tumor upon removal to improve fixation. Fixation times have been defined as no less than 6 hours, and no greater than 72 hours for all three markers. (previously, it was a max of 48 hours for Her2) If you are not recording the end time, then how can you report the Max fixation time? I hope this helps - Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu Sent: Saturday, February 15, 2014 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 123, Issue 15 Message: 4 Date: Fri, 14 Feb 2014 13:18:18 -0700 From: Glenn Hauck Subject: [Histonet] End time for fixation of breast tissue Is there someone out there that can help me find out if there is any value in recording the end time for fixation of breast tissue in 10% formalin? We at present record the devitalization time, the time tissue is put into fixative and the time the tissue is cut into and exposed to 10% formalin as well as the time the processor is started Thanks Glenn Hauck Charge Tecnologist Pathology QueenElizabeth II Hospital Grande Prairie, AB T8V 2E8 **** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Mon Feb 17 09:05:38 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 17 09:05:58 2014 Subject: [Histonet] Mineral Oil in Microwave Processing. In-Reply-To: <3566D9E34287BE4B95372179009446A01A47D4F6@EXCHANGE.fmhnt.fmh.org> Message-ID: <1392649538.90767.YahooMailBasic@web120406.mail.ne1.yahoo.com> Mineral oil per se CANNOT be used in a MW oven because mineral oil = paraffin of low mollecular weight and as such is "microwaves transparent" = it is a non polar substance and therefore cannot be "excited" (heated) by the microwaves. That is why the Sakura Excel has 2 chambers: one to dehydrate using MW and the other "conventional" to clear and infiltrate using mineral oil and paraffin. Ren? J. -------------------------------------------- On Mon, 2/17/14, Campbell, Tasha M. wrote: Subject: RE: [Histonet] Mineral Oil in Microwave Processing. To: "Cesar Francisco Romero" , histonet@lists.utsouthwestern.edu Date: Monday, February 17, 2014, 7:09 AM I have never heard of using mineral oil in microwave processing.? I only use ethyl and isopropyl and paraffin. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cesar Francisco Romero Sent: Sunday, February 16, 2014 9:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mineral Oil in Microwave Processing. Dear People from Histonet Do you still use Mineral Oil in your Microwave Processing Protocol ? Or you only use ethyl alcohol ~ Isopropyl alcohol and Paraffin . I will apreciate any advice. Thank you all Cesar Romero Buenos Aires Argentina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Feb 17 09:08:26 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Feb 17 09:08:30 2014 Subject: [Histonet] Mineral Oil in Microwave Processing. In-Reply-To: <1392649538.90767.YahooMailBasic@web120406.mail.ne1.yahoo.com> References: <3566D9E34287BE4B95372179009446A01A47D4F6@EXCHANGE.fmhnt.fmh.org>, <1392649538.90767.YahooMailBasic@web120406.mail.ne1.yahoo.com> Message-ID: It was part of the paraffin Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 17 Feb 2014 07:05:38 -0800 > From: rjbuesa@yahoo.com > To: chesarato@hotmail.com; histonet@lists.utsouthwestern.edu; tmcampbell@fmh.org > Subject: RE: [Histonet] Mineral Oil in Microwave Processing. > CC: > > Mineral oil per se CANNOT be used in a MW oven because mineral oil = paraffin of low mollecular weight and as such is "microwaves transparent" = it is a non polar substance and therefore cannot be "excited" (heated) by the microwaves. > That is why the Sakura Excel has 2 chambers: one to dehydrate using MW and the other "conventional" to clear and infiltrate using mineral oil and paraffin. > Ren? J. > -------------------------------------------- > On Mon, 2/17/14, Campbell, Tasha M. wrote: > > Subject: RE: [Histonet] Mineral Oil in Microwave Processing. > To: "Cesar Francisco Romero" , histonet@lists.utsouthwestern.edu > Date: Monday, February 17, 2014, 7:09 AM > > I have never heard of using mineral > oil in microwave processing. I only > use ethyl and isopropyl and paraffin. > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 (w) > 304-685-9307 (c) > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Cesar > Francisco Romero > Sent: Sunday, February 16, 2014 9:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Mineral Oil in Microwave Processing. > > > > > Dear People from > Histonet > > > Do you still use > Mineral Oil in your Microwave Processing Protocol ? > > > Or you only use > ethyl alcohol ~ Isopropyl alcohol and Paraffin . > > > I will apreciate > any advice. > > > Thank you all > > > Cesar Romero > > > Buenos Aires > > > Argentina > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Mon Feb 17 12:07:22 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Feb 17 12:07:28 2014 Subject: [Histonet] MUC IHC Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0226C060@HHCEXCHMB03.hhcsystem.org> For those of you doing IHC for epithelial mucin gene proteins (MUC) on FFPE tissue, whose antibodies are you using? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Richard.Cartun <@t> hhchealth.org Mon Feb 17 16:40:22 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Feb 17 16:40:29 2014 Subject: [Histonet] Immunocal Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0226C13A@HHCEXCHMB03.hhcsystem.org> I'm looking for information (good or bad) on the use of "Immunocal" for bone marrow biopsy specimens. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From liz <@t> premierlab.com Mon Feb 17 16:57:36 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Feb 17 16:57:42 2014 Subject: [Histonet] RE: Immunocal In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E0226C13A@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E0226C13A@HHCEXCHMB03.hhcsystem.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05463@SBS2K8.premierlab.local> Richard We have used this in the past for research samples and it works just fine, I think its expensive. Its formic acid based, probably a buffered formic acid solution. We just use unbuffered 10% formic acid that we make in house and that seems to work well for us. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Monday, February 17, 2014 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunocal I'm looking for information (good or bad) on the use of "Immunocal" for bone marrow biopsy specimens. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcman2010 <@t> hotmail.com Tue Feb 18 00:14:59 2014 From: ihcman2010 <@t> hotmail.com (Glen) Date: Tue Feb 18 00:15:14 2014 Subject: [Histonet] How do you do Histonet? Message-ID: <52EABE1501C5CE77@smtp201.alice.it> (added by postmaster@alice.it) http://asociacionriocavia.com/rd/hotnews.php?gptephugb1297xcyywmv .............................. ihcman2010@hotmail.com Does it make you kind of mad that you have to reason with me while I'm wearing this enormous balloon hat? -- Zug From Sarah_Mack <@t> urmc.rochester.edu Tue Feb 18 08:06:24 2014 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Tue Feb 18 08:07:38 2014 Subject: [Histonet] New York State Histotechnological Society Educational Scholarship Message-ID: Good morning! All members of New York State Histotechnological Society have the opportunity to apply for corporate educational scholarships, currently there are several available. These scholarships can be used to defray educational expenses, attend professional meetings or buy educational material. I have attached the link to our website, please take a look at the scholarships that are available and apply! Please note the application deadline is March 31, 2014. http://www.nyhisto.org/awards/2013-educational-scholarships/ Kind Regards, Sarah Mack NYSHS Membership Secretary University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 From imhyper13 <@t> aol.com Tue Feb 18 08:51:55 2014 From: imhyper13 <@t> aol.com (imhyper13@aol.com) Date: Tue Feb 18 08:51:59 2014 Subject: [Histonet] Does anyone use grossing canned messages? Message-ID: <8D0FAD019A1E3A2-EC0-15A8@webmail-m232.sysops.aol.com> Hello All, We are currently exploring changing our LIS, and one we are looking at relies heavily on the use of canned messages for grossing. We have a few canned messages that we currently use, but nothing like what we are seeing with this new system. Does anyone out there use these, and if so, how do you create them? For example, do you just have a generic colon, or do you have one for cancer, diverticulitis, etc? Any information would be greatly appreciated. Thank you From Glenn.Hauck <@t> albertahealthservices.ca Tue Feb 18 10:01:00 2014 From: Glenn.Hauck <@t> albertahealthservices.ca (Glenn Hauck) Date: Tue Feb 18 10:01:10 2014 Subject: [Histonet] RE: End time for fixation of breast tissue In-Reply-To: References: <38FA8FCA474F834CB9B90590D7C72390015A681C5C4B@EXMBX4C.crha.bewell.ca> <761E2B5697F795489C8710BCC72141FF36758028@ex07.net.ucsf.edu> Message-ID: <38FA8FCA474F834CB9B90590D7C72390015A681C5C4D@EXMBX4C.crha.bewell.ca> Thanks all Glenn -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems@emoryhealthcare.org] Sent: Monday, February 17, 2014 6:54 AM To: 'Morken, Timothy'; Glenn Hauck Cc: Histonet Subject: RE: End time for fixation of breast tissue We have the nurses put the time the breast is placed in formalin on the requisition as well as the time it is removed from the body. Cold ischemic time must be documented as well. This is important when the tissue goes for xray and is not immediately placed in formalin. Also, the limit for time in formalin is 72 hours, so you need to document - even if more than 72 hours. Happy Monday!! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, February 14, 2014 3:40 PM To: 'Glenn Hauck' Cc: Histonet Subject: [Histonet] RE: End time for fixation of breast tissue Glenn, You do need to show it was in formalin for at least 6 hours. we work backwards from the time on the processor that the tissue leaves the formalin. For us 3pm is the cutoff for breast tissue to get 6 hours fixation. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Glenn Hauck Sent: Friday, February 14, 2014 12:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] End time for fixation of breast tissue Is there someone out there that can help me find out if there is any value in recording the end time for fixation of breast tissue in 10% formalin? We at present record the devitalization time, the time tissue is put into fixative and the time the tissue is cut into and exposed to 10% formalin as well as the time the processor is started Thanks Glenn Hauck Charge Tecnologist Pathology QueenElizabeth II Hospital Grande Prairie, AB T8V 2E8 780-538-7429 Work Main 780-538-7184 Work Office glenn.hauck@albertahealthservices.ca ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From hsmith <@t> wakehealth.edu Tue Feb 18 10:18:01 2014 From: hsmith <@t> wakehealth.edu (Hilary Smith) Date: Tue Feb 18 10:18:17 2014 Subject: [Histonet] BBB permeability marker Message-ID: Hello - does anyone have a recommendation for a marker of blood brain barrier disruption in fresh frozen cryostat sectioned tissue? Can I postfix and immunostain for albumin extravasation, or does albumin easily diffuse away from the vessels post-mortem? The tissue was flash frozen in isopentane, however it was thaw-mounted onto slides before overnight dessication and refreezing. Thanks, Hilary From JMitchell <@t> uwhealth.org Tue Feb 18 10:27:25 2014 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Tue Feb 18 10:27:29 2014 Subject: [Histonet] 2014 Tri-State Histology Symposium Message-ID: <16F90B93CA23D446980B3D591FD02DAD0DA99A@UWHC-MBX14.uwhis.hosp.wisc.edu> Registration is open for the Minnesota, Wisconsin and Iowa 2014 Tri-State Spring Histology Symposium that will be held at the DoubleTree Hotel Rochester, Minnesota April 30-May 2. There is an outstanding program of seminars and workshops that begin Wednesday afternoon April 30th and conclude at noon on Friday May 2nd. Join us for education, vendor displays, socializing and histotech camaraderie as we take a journey on a "Passport to Histology Success" in 2014. For program, registration and vendor/exhibit information contact the following representatives: Minnesota: Sheri Blair (sheriblair1@netzero.net) Wisconsin: Jean Mitchell (jmitchell@uwhealth.org) Iowa: Judi Stasko (judith.stasko@ars.usda.gov) Vendor/Exhibit information: Dawn Schneider (dawn.schneider@ministryhealth.org) From BDeBrosse-Serra <@t> isisph.com Tue Feb 18 10:46:22 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Feb 18 10:46:35 2014 Subject: [Histonet] RE: Immunocal In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E0226C13A@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E0226C13A@HHCEXCHMB03.hhcsystem.org> Message-ID: I had good luck with Chelator Cal from BBC Biochemical. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Monday, February 17, 2014 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunocal I'm looking for information (good or bad) on the use of "Immunocal" for bone marrow biopsy specimens. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Tue Feb 18 13:19:07 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Feb 18 13:19:13 2014 Subject: [Histonet] Neutralization of AZF Message-ID: It's been awhile but we have a procedure listed for neutralization of AZF fixative. One of the ingredients is listed as simply sodium carbonate. Does anyone know if this reagent is correct or should it be sodium bicarbonate? We are thinking it should be sodium anhydrous carbonate but can't pull up the original article on the internet. Could some share this information or send me their method of neutralization? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From BZIMMERM <@t> gru.edu Tue Feb 18 13:51:51 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Feb 18 13:51:58 2014 Subject: [Histonet] HISTOPALOOZA APRIL 25 - 27, 2014 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8115C4@EX-MLB-03.ad.georgiahealth.edu> Here's another teaser for the upcoming Histopalooza being held at The Lodge and Spa at Callaway Gardens. We're excited to have Wanda Jones, 2013 NSH HT of the year, conducting a workshop. This workshop is titled " Becoming a Better Person in the Workplace and in Life". This workshop is for everyone who wants to have a better understanding of what motivates us-why employee involvement and recognition is so important in today's workforce and everyday life. Why do hard times happen to good people. This workshop is on Friday, April 25 from 1:30 to 3:00. I find it interesting that this workshop is followed on Saturday at 8:30 am by a workshop titled "Because Strangling Isn't An Option; Keeping your Sanity While Dealing with the Problem Employee. I suggest attending the workshop on Friday so that on Saturday morning, you will feel even better about not strangling anyone. Shhhh, the Godfather is teaching the strangling workshop. Don't let him take you on a fishing boat in the middle of the lake. Just sayin... don't strangle me Wanda! Do you know Wanda?? From thomas.huynh <@t> mdanderson.org Tue Feb 18 13:52:31 2014 From: thomas.huynh <@t> mdanderson.org (Huynh,Thomas) Date: Tue Feb 18 13:52:41 2014 Subject: [Histonet] Moh's Job Message-ID: Hello everyone in the Histoland I am posting a job for a friend; they are looking for a fulltime Mohs' tech at a local dermatology practice in the Houston Texas Medical Center. Please contact Ms. Stephanie Huff at 281-799-0521. Thomas Thomas Huynh HT(ASCP) Dept. of Pathology Histology/ Bone Lab Tel. 713-745-4759 Fax. 713-792 2046 From asanjeet <@t> yahoo.com Tue Feb 18 14:48:10 2014 From: asanjeet <@t> yahoo.com (Sanjeet Dhirubhai) Date: Tue Feb 18 14:51:03 2014 Subject: [Histonet] Re: Lens vs sponges Message-ID: <1392756490.23876.YahooMailBasic@web161005.mail.bf1.yahoo.com> Hi Histo Folks, We are debating on what we should use during grossing either lens papers or sponges. We are currently using sponges. And in the near future we will be moving to another Lab, basically a merger of 2 Histo depts. The other Lab uses lens papers. I need to know the pros and cons of Sponges verses lens paper. with sponges there is carrier over of reagents. Is there any other or any major disadvantages of using sponges. Our reagent maintenance is done everyday and is standard. We do not go by the reagent management alerts from the Tissue Processor. Help Regards, ? Sanjeet Dhirubhai? ? ? ? ? -------------------------------------------- On Tue, 2/18/14, histonet-request@lists.utsouthwestern.edu wrote: Subject: Histonet Digest, Vol 123, Issue 4 To: histonet@lists.utsouthwestern.edu Received: Tuesday, February 18, 2014, 12:39 PM Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ???1. Price for preparing IHC slides (Ann Specian) ???2. Re: Price for preparing IHC slides (Will Chappell) ???3. IHC cartilage (Victoria Rimkunas) ???4. Re: Turn Around Time (Jay Lundgren) ???5. dust-free coverslips - do they really exist? (Connolly, Brett M) ???6. Re: Turn Around Time (Rene J Buesa) ???7. RE: Price for preparing IHC slides (Joe W. Walker, Jr.) ???8. Re: Price for preparing IHC slides (Michael Farmer) ???9. RE: Turn Around Time (Martha Ward-Pathology) ? 10. Re: Turn Around Time (Bob Richmond) ? 11. RE: Turn Around Time (Joe W. Walker, Jr.) ? 12. Re: Turn Around Time (Rene J Buesa) ? 13. RE: How to De-stain trichrome (Tony Henwood (SCHN)) ? 14. Re: Histonet Digest, Vol 123, Issue 3 (Madeleine Huey) ? 15. Re: Price for preparing IHC slides (Mike Thompson) ---------------------------------------------------------------------- Message: 1 Date: Mon, 3 Feb 2014 13:03:28 -0500 (EST) From: Ann Specian Subject: [Histonet] Price for preparing IHC slides To: histonet@lists.utsouthwestern.edu Message-ID: <8D0EF215FBFEBF2-1E68-1A6E4@webmail-m233.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Can anyone tell me the average cost for preparing an IHC slide? thanks, Ann ------------------------------ Message: 2 Date: Mon, 3 Feb 2014 13:08:28 -0500 From: Will Chappell Subject: Re: [Histonet] Price for preparing IHC slides To: Ann Specian Cc: "histonet@lists.utsouthwestern.edu" ??? Message-ID: <77A77586-CF08-42B0-BABA-B972F803EA17@yahoo.com> Content-Type: text/plain;??? charset=us-ascii A lab I used in Southern California charged $35 per stain? for most antibodies. Sent from my iPhone > On Feb 3, 2014, at 1:03 PM, Ann Specian wrote: > > > Can anyone tell me the average cost for preparing an IHC slide? > thanks, Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 3 Feb 2014 18:29:18 +0000 From: Victoria Rimkunas Subject: [Histonet] IHC cartilage To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <2661A06F49EDCF41ABFEE80C2727C2237204B5A6@MEP-MBX2.mack.local> Content-Type: text/plain; charset="us-ascii" Does anyone have experience or a protocol for IHC staining on cartilage? ------------------------------ Message: 4 Date: Mon, 3 Feb 2014 12:31:44 -0600 From: Jay Lundgren Subject: Re: [Histonet] Turn Around Time To: joelle weaver Cc: "histonet@lists.utsouthwestern.edu" ??? ,??? Tom McNemar ??? Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Yesterday. ? ???Sincerely? ? ? ? ? ? Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Feb 3, 2014 at 11:56 AM, joelle weaver wrote: > ditto. Only FISH is allowed to take longer > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: TMcNemar@lmhealth.org > > To: drbugge@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Mon, 3 Feb 2014 12:49:14 -0500 > > Subject: RE: [Histonet] Turn Around Time > > CC: > > > > Our benchmark is 24 hour TAT for 80% of cases.? We provide same-day > service for recuts and most in-house special stains. > > > > Tom McNemar, HT(ASCP) > > Histology Supervisor > > Licking Memorial Health Systems > > (740) 348-4163 > > (740) 348-4166 > > tmcnemar@lmhealth.org > > www.LMHealth.org > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge > > Sent: Monday, February 03, 2014 12:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Turn Around Time > > > > Hello Histonet > > > > I am just curious what the standard for Turn Around Time is for most > labs. > > I think a two day turn around time from the time the biopsy gets to the > lab > > to the time the pathologist signs out a case is pretty fast. > > > > Thanks for your input. > > -- > > Dawn R Bugge > > Seattle Histology > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Mon, 3 Feb 2014 13:35:22 -0500 From: "Connolly, Brett M" Subject: [Histonet] dust-free coverslips - do they really exist? To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset="us-ascii" What's your favorite?? It gets frustrating when I need to capture low power images and there coverslips are dirty right out of the box. Fisherfinest Premium isn't bad, but I am wondering if there is something better??? Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Mon, 3 Feb 2014 10:38:14 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Turn Around Time To: Tom McNemar , 'Dawn Bugge' ??? ,??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <1391452694.39779.YahooMailNeo@web120405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 In the US the "standard" (or most frequent) TAT is 24 hours. In other countries the TAT varies greatly, in extreme cases up to 1 week for surgicals! Ren? J. ________________________________ From: Tom McNemar To: 'Dawn Bugge' ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 3, 2014 12:49 PM Subject: RE: [Histonet] Turn Around Time Our benchmark is 24 hour TAT for 80% of cases.? We provide same-day service for recuts and most in-house special stains. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 3 Feb 2014 18:43:49 +0000 From: "Joe W. Walker, Jr." Subject: RE: [Histonet] Price for preparing IHC slides To: Ann Specian , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <3C2378778400AD448ADA6FD6BDB7CCCC181D2DCB@RRMBX03.rrmc.local> Content-Type: text/plain; charset="iso-8859-1" I believe that this would be highly dependent on the antibodies used, how often the antibodies are used, slide costs, tech costs, overhead, etc.? What would be average for 1 institution might be above or below average for another. What are you trying to determine, Ann? Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790? F: 802.747.6525 Email joewalker@rrmc.org? ? www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Monday, February 03, 2014 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Price for preparing IHC slides Can anyone tell me the average cost for preparing an IHC slide? thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ------------------------------ Message: 8 Date: Mon, 3 Feb 2014 13:59:32 -0500 From: Michael Farmer Subject: Re: [Histonet] Price for preparing IHC slides To: Ann Specian Cc: histonet@lists.utsouthwestern.edu Message-ID: <35E9AD8D-1846-430D-BD59-71BB57DC7194@mcevoyandfarmer.com> Content-Type: text/plain; charset="iso-8859-1" This is a fascinating question, Ann - I've been studying this topic for the last couple of years in five countries. While I do not yet understand this complex phenomenon as well as I would wish to, these are my impressions about life here in the US. It is a ridiculous tale, but I will tell it to you... The smartest shoppers who have the biggest contracts (you can guess who those might be) are paying $5-10 for their highest-volume slides? - ER, PR, HER-2 and a few others - but more like? $10-15 for most of their menus. The smallest IHC customers think they are paying 20-something per slide, but they are actually paying $30-$40 per slide - and more in many cases. How this discrepancy? Two reasons: first, because the suppliers (I can't quite remember their names right now, please pardon my senior moment) are highly-skilled at making their price lists and service contracts as eye-glazingly complicated as possible. And second, because immutable human nature compels many mere mortals to underestimate their costs, particularly while they are still trying to rationalize a bad investment they made some time ago I'm pretty sure that American labs (excluding Canada, mind you) spent? $700-750m with the IHC companies in 2013. I think that between 28m and 32 million IHC slides were run last year in the US. If you want to slice those estimates down the middle you'll come up with maybe $24/slide, once every penny of waste, service, and sub-optimal operating procedures are truly accounted for. There you have one of the most useless averages you'll ever hear. There are plenty of contracts out there in vast middle America at every price point between $7 and $30 per slide. You pay what your volume earns you, unless of course you happen to be in the market for a tissue processor or primary stainer at the time you're haggling with the IHC companies. I'll remember their names if you give me another a minute. That's the way it was last year. WIth these new codes, lots of small labs will be priced out of the IHC business by summertime, and the big labs that remain will have negotiated prices within a narrower range. That'll be a fast-moving target. Since I didn't see you in the crowd at the funeral of 88342 last month, I am attaching below our chronicle of the event. I'm always happy to banter about this with anyone who thinks the topic is interesting. Sincerely, Michael Farmer McEvoy & Farmer Pathology www.mcevoyandfarmer-pathology.com 415-994-8852 "Those who seek the truth doubt those who find it" - Andr? Gide -------------- next part -------------- On Feb 3, 2014, at 1:03 PM, Ann Specian wrote: > > Can anyone tell me the average cost for preparing an IHC slide? > thanks, Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 3 Feb 2014 19:05:47 +0000 From: Martha Ward-Pathology Subject: RE: [Histonet] Turn Around Time To: Dawn Bugge , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? ??? Content-Type: text/plain; charset="iso-8859-1" While I recognize the need for a quick result in some cases, I also subscribe to the theory that "speed kills".???I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience.? ? However that is another discussion altogether.? That said, our institution shoots for the 80% in 24 hours as well. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 3 Feb 2014 14:50:45 -0500 From: Bob Richmond Subject: [Histonet] Re: Turn Around Time To: "Histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Dawn R. Bugge at Seattle WA Histology asks: >>I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast.<< Normally I'd receive the specimen on Monday and gross it, have it processed overnight, get the slides some time Tuesday morning, and sign the case out well before close of business on Tuesday. Exceptions are when the specimen needs to fix overnight before I gross it (bowel resection) or after I gross it (fatty breast tissue, amputation specimens, autopsies), or when a specimen of more than minimal size (or received after 1400) requires decalcification. What I say goes. Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 11 Date: Mon, 3 Feb 2014 19:54:21 +0000 From: "Joe W. Walker, Jr." Subject: RE: [Histonet] Turn Around Time To: Martha Ward-Pathology , Dawn Bugge ??? , "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <3C2378778400AD448ADA6FD6BDB7CCCC181D4260@RRMBX03.rrmc.local> Content-Type: text/plain; charset="iso-8859-1" I agree with you, Martha.? That is why we look at it from an average of all cases for the month.? We definitely encounter cases that are difficult and require more time with our pathologists.? We track the TAT for statistical purposes only.? The CAP (for those who are CAP accredited) has dropped this from their checklist for surgical cases.? It seems to only really apply to autopsy cases now but these has a much different TAT target. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790? F: 802.747.6525 Email joewalker@rrmc.org? ? www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Monday, February 03, 2014 2:06 PM To: Dawn Bugge; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Turn Around Time While I recognize the need for a quick result in some cases, I also subscribe to the theory that "speed kills".???I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience.? ? However that is another discussion altogether.? That said, our institution shoots for the 80% in 24 hours as well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard? \? Winston-Salem, NC 27157 p 336.716.2109? \? f 336.716.5890 mward@wakehealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ------------------------------ Message: 12 Date: Mon, 3 Feb 2014 13:37:49 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Turn Around Time To: Martha Ward-Pathology ,??? Dawn Bugge ??? ,??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <1391463469.21418.YahooMailNeo@web120406.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Martha: So, do you mean that you are not satisfied with the quality of your slides? If you are, I just do not understand your concerns about a 24 h TAT! The fundamental issue is to?develop adequate protocols assuring quality as well as a?convenient (24 h) TAT. Just my 3 cents (after inflation!) Ren? J. ________________________________ From: Martha Ward-Pathology To: Dawn Bugge ; "histonet@lists.utsouthwestern.edu" Sent: Monday, February 3, 2014 2:05 PM Subject: RE: [Histonet] Turn Around Time While I recognize the need for a quick result in some cases, I also subscribe to the theory that "speed kills".? I'm not sure that these quick TATs are always medically necessary, but rather more of a convenience.? ? However that is another discussion altogether.? That said, our institution shoots for the 80% in 24 hours as well. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Monday, February 03, 2014 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Turn Around Time Hello Histonet I am just curious what the standard for Turn Around Time is for most labs. I think a two day turn around time from the time the biopsy gets to the lab to the time the pathologist signs out a case is pretty fast. Thanks for your input. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 3 Feb 2014 21:58:51 +0000 From: "Tony Henwood (SCHN)" Subject: RE: [Histonet] How to De-stain trichrome To: "'Hans B Snyder'" , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00AD9EE@xmdb04.nch.kids> Content-Type: text/plain; charset="us-ascii" Hi Hans, It is possible, though it will depend on what you want to do to the slides afterword. The problem arises from the use of phosphotungstic or phosphmolybdic acid which is used for differentiation and mordanting in the trichrome stains like Masson's. These are very difficult to reverse (anyone trying to restain PAP smears might know the problem). This may cause problems. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hans B Snyder Sent: Tuesday, 4 February 2014 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to De-stain trichrome Does anyone know how to destain Massons trichrome? Is it possible? Thank you Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 14 Date: Mon, 3 Feb 2014 14:26:00 -0800 From: Madeleine Huey Subject: [Histonet] Re: Histonet Digest, Vol 123, Issue 3 To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Dawn, Our TAT is 94% with 24 hours & 98% within 48 hours (~ 6% complicated cases need more IPOX/Molecular Study/FACS & etc.). Madeleine Superviser - Pathology (IPOX & Histology) madeleine_h@elcaminohospital.org From: histonet-bounces@lists.utsouthwestern.edu [mailto: histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge > Sent: Monday, February 03, 2014 12:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Turn Around Time > > Hello Histonet > > I am just curious what the standard for Turn Around Time is for most labs. > I think a two day turn around time from the time the biopsy gets to the lab > to the time the pathologist signs out a case is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology On Mon, Feb 3, 2014 at 10:00 AM, wrote: > Send Histonet mailing list submissions to >? ? ? ???histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >? ? ? ???http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to >? ? ? ???histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at >? ? ? ???histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >? ? 1. Re: Gross lab seniors (WILLIAM DESALVO) >? ? 2. Re: protocols to fix insect wings that are quite hirsute (Damien) >? ? 3. RE: Ventana LEAN (Tim Higgins) >? ? 4. RE: RE: Ventana LEAN (Rathborne, Toni) >? ? 5. How to De-stain trichrome (Hans B Snyder) >? ? 6. Histotech position FT West Palm Beach (nicole@dlcjax.com) >? ? 7. anyone need a glass cover slipper (Curt) >? ? 8. On-call position for Histology Assistant in Phoenix AZ (Jill Cox) >? ? 9. RE: RE: Ventana LEAN (Eytalis, Robert A) >???10. Turn Around Time (Dawn Bugge) >???11. RE: Turn Around Time (Tom McNemar) >???12. RE: Turn Around Time (joelle weaver) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 2 Feb 2014 18:34:15 +0000 > From: WILLIAM DESALVO > Subject: Re: [Histonet] Gross lab seniors > To: E. Wayne Johnson ??? > Cc: histonet , " Vickroy,? Jim " >? ? ? ??? > Message-ID: > Content-Type: text/plain; charset="utf-8" > > Absolutely the Face velocity changes with the work area set-up. This is > why I like to make sure? the air flow away is maintained at a minimum. Most > grossing stations have large working area and often the flow away from the > grosser is not checked, just the vent opening draw. Most important is to > set up a process and then regularly check. > > > > > > > Sent from Windows Mail > > > > > > From: E. Wayne Johnson ????????? > Sent: ???Saturday???, ???February??? ???1???, ???2014 ???3???:???12??? ???PM > To: WILLIAM DESALVO > Cc: Vickroy, Jim, histonet > > > > > > Face velocity is simply the airflow rate in CFM divided by the area of > the hood opening in square feet. > > A smaller opening at the same flow rate gives a higher face velocity. > > Titanium tetrachloride in a small plastic squeeze bottle can be used to > generate "smoke". > > > On 3:59 AM, WILLIAM DESALVO wrote: > > We use a company called C-Scan Technologies, Phoenix, AZ. The way they > test all our gross dissection stations is by testing for directional or > smoke containment and face velocity. We also check th they external pathway > is clear and if the unit has a filtering system, the filters are changed > regularly. The air flow measurement is Feet per minute (FPM) for face > velocity and includes width, height, depth and total square ft for the > working area. They exhaust flow in CFM. Face velocity minimum requirement > is 100 fpm, exhaust flow requirement is>500 cfm. Face velocity fluctuates > depending on the room and the air exchange rate for the area. I have always > felt the face velocity is most important to gross dissection personnel. > There needs to be adequate draw away from the employee, no matter the > physical conditions of the room. > > > > William DeSalvo, BS HTL(ASCP) > > Production Manager-Anatomic Pathology > > Chair, NSH Quality Management Committee > > Owner/Consultant, Collaborative Advantage Consulting > > > > > > > >> From: Vickroy.Jim@mhsil.com > >> To: histonet@lists.utsouthwestern.edu > >> Date: Fri, 31 Jan 2014 12:46:08 -0600 > >> Subject: [Histonet] Gross lab seniors > >> > >> > >> We have several gross lab senior grossing stations that are vented > outside.? Our engineering asked today whether the airflow should be checked > yearly like other exhaust hoods.???Problem is there is not a door like > other hoods of course and how would you measure the airflow????Recommended > airflow is 500cfm however clearly the airflow at the working surface is not > anything close to that.???I wondered how anybody else monitors the gross > lab seniors or do they at all.???CAP used to ask about documentation for > checking hoods however I can't recall them ever checking on grossing > stations.? We change filters annually? only since they are vented outside. > >> > >> Jim > >> > >> James Vickroy BS, HT(ASCP) > >> > >> Surgical? and Autopsy Pathology Technical Supervisor > >> Memorial Medical Center > >> 217-788-4046 > >> > >> > >> ________________________________ > >> This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should delete > this message. Any disclosure, copying, or distribution of this message, or > the taking of any action based on it, is strictly prohibited. > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 3 Feb 2014 05:40:33 -0500 > From: Damien > Subject: Re: [Histonet] protocols to fix insect wings that are quite >? ? ? ???hirsute > To: Jack Ratliff > Cc: "histonet@lists.utsouthwestern.edu" >? ? ? ??? > Message-ID: >? ? ? ??? vq+Qrw55qKhWdQ@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Jorge, > > I'd be happy to help you if I can. Please feel free to message me. > > Best, > Damien L > > > On Sun, Feb 2, 2014 at 10:45 AM, Jack Ratliff >wrote: > > > This sounds like a question for the insect histology expert, Damien > > Laudier! He monitors this site so I am sure he will respond to you > > privately. :) > > > > Jack > > > > > On Feb 1, 2014, at 5:00 PM, "Jorge A. Santiago-Blay" < > > blayjorge@gmail.com> wrote: > > > > > > Hello: > > > > > > Can someone point me on the direction of protocols to fix insect wings > > that > > > are quite hirsute. I would like to increase the likelihood of the > > > preservative actually going through the carpet of setae to actually fix > > the > > > interior of the wing. Thanks for any help. > > > > > > Sincerely, > > > > > > Jorge > > > > > > Jorge A. Santiago-Blay, PhD > > > blaypublishers.com > > > http://blayjorge.wordpress.com/ > > > http://paleobiology.si.edu/staff/individuals/santiagoblay.html > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Damien Laudier > Laudier Histology > www.LaudierHistology.com > > > ------------------------------ > > Message: 3 > Date: Mon, 3 Feb 2014 07:58:51 -0600 > From: Tim Higgins > Subject: [Histonet] RE: Ventana LEAN > To: "histonet@lists.utsouthwestern.edu" >? ? ? ??? > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > They come in, give their opinions on how to make your workflow better.? I > didn't get much from it, we didn't make any changes, more of a selling tool > in my opion.? I guess if your workflow was terrible, they might be able to > offer good advice. > > On a positive, they were all very nice. > > Thank, > Tim H. > > > > ------------------------------ > > Message: 4 > Date: Mon, 3 Feb 2014 14:32:59 +0000 > From: "Rathborne, Toni" > Subject: RE: [Histonet] RE: Ventana LEAN > To: "'Tim Higgins'" , >? ? ? ???"histonet@lists.utsouthwestern.edu" >? ? ? ??? > Message-ID: >? ? ? ???< > 3AD061FE740D464FAC7BF6B5CFB75707A95B53C7@SMCMAIL01.somerset-healthcare.com > > > > Content-Type: text/plain; charset="us-ascii" > > Another benefit of someone coming in from the outside (Leica did the same > for us), is that since this is what these people do every day, management > is more likely to listen to their suggestions. If it's the same as yours > then that would reinforce your current plans. If it's different than what > you had in mind, they will generally try to help with what you want to do. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins > Sent: Monday, February 03, 2014 8:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Ventana LEAN > > They come in, give their opinions on how to make your workflow better.? I > didn't get much from it, we didn't make any changes, more of a selling tool > in my opion.? I guess if your workflow was terrible, they might be able to > offer good advice. > > On a positive, they were all very nice. > > Thank, > Tim H. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Mon, 3 Feb 2014 09:53:45 -0500 > From: Hans B Snyder > Subject: [Histonet] How to De-stain trichrome > To: "histonet@lists.utsouthwestern.edu" >? ? ? ??? > Message-ID: > Content-Type: text/plain;? ? ???charset=us-ascii > > Does anyone know how to destain Massons trichrome? Is it possible? > > Thank you > > Histologistics > Hans B Snyder > 508.308.7800 > Hans@histologistics.com > > > > ------------------------------ > > Message: 6 > Date: Mon, 3 Feb 2014 15:19:07 +0000 (UTC) > From: nicole@dlcjax.com > Subject: [Histonet] Histotech position FT West Palm Beach > To: histonet@lists.utsouthwestern.edu > Message-ID: <1940731504.67335.1391440747343.JavaMail.mail@webmail17> > Content-Type: text/plain; charset="UTF-8" > > >? ???Posting? for???I/MD? Path labs? West Palm Beach, Florida? Looking for >? ? full? time? Histotech.? Dermpath? lab.? Please? contact? Dr.? Morales. >? ? 1(561)653-8005 > > > ------------------------------ > > Message: 7 > Date: Mon, 3 Feb 2014 17:06:56 +0000 > From: Curt > Subject: [Histonet] anyone need a glass cover slipper > To: "Histonet@lists.utsouthwestern.edu" >? ? ? ??? > Message-ID: > > <9C8F910F72893643B3C3793C3D67132B01440853@PATHOLOGYSERVER.pathologyarts.local > > > > Content-Type: text/plain; charset="us-ascii" > > We have on old Hacker glass cover slipper, I think it's? a Hacker.... The > name plate on the front says Meisei but I'm told it's a Hacker. Anyway, the > model number: RCM-3660 if that's helpful. > > I'm not looking to make a bunch of money, so many people here have been > helpful over the years from knowledge and advice to helping me out with > control tissue. > > If someone needs one, I'd be willing to help out a bit, just need to cover > the expense of shipping and packing. > > Let me know, I can snap a picture if desired. We recently had is serviced > and it's said to be working fine by the technician. > > > Curt > > > > ------------------------------ > > Message: 8 > Date: Mon, 3 Feb 2014 08:58:56 -0800 (PST) > From: Jill Cox > Subject: [Histonet] On-call position for Histology Assistant in >? ? ? ???Phoenix AZ > To: "Histonet@Lists. Edu" > Message-ID: >? ? ? ???<1391446736.89252.YahooMailBasic@web160701.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Hi Netters, > We are looking for an on-call histology assistant to cover for vacations > and sick leave, already have a few days in March/April. We are a small > Dermatology lab in NW Peoria AZ, great working conditions and people. Very > nice place to work. Hours are from 7:30-4:00PM when needed.? Email me for > more details. > Thank you, Jill > > Arrowhead Dermatology > > > > > ------------------------------ > > Message: 9 > Date: Mon, 3 Feb 2014 17:00:28 +0000 > From: "Eytalis, Robert A" > Subject: RE: [Histonet] RE: Ventana LEAN > To: "Rathborne, Toni" , "'Tim >? ? ? ???Higgins'"? ? ???, " > histonet@lists.utsouthwestern.edu" >? ? ? ??? > Message-ID: >? ? ? ???< > F3024E541CBF9049BD4A805E1646204A6F8CE5A0@RHCMBX02.RiversideHealthCare.net> > > Content-Type: text/plain; charset="us-ascii" > > Depends on if your management is skeptical of vendors. Mine automatically > assume that they are marketing to us. > > Robert A. Eytalis > Laboratory Manager > robert-eytalis@riversidehealthcare.net > Phone: (815) 935-7256 ext. 5186 >? ? ? ? ? ? ? ???(815) 935-7535 > Fax? ? ? ???(815) 935-7068 > > Riverside Medical Center > 350 N. Wall Street - Kankakee, IL 60901 > > > > http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f | > http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of Rathborne, Toni [ > trathborne@somerset-healthcare.com] > Sent: Monday, February 03, 2014 8:32 AM > To: 'Tim Higgins'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Ventana LEAN > > Another benefit of someone coming in from the outside (Leica did the same > for us), is that since this is what these people do every day, management > is more likely to listen to their suggestions. If it's the same as yours > then that would reinforce your current plans. If it's different than what > you had in mind, they will generally try to help with what you want to do. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Higgins > Sent: Monday, February 03, 2014 8:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Ventana LEAN > > They come in, give their opinions on how to make your workflow better.? I > didn't get much from it, we didn't make any changes, more of a selling tool > in my opion.? I guess if your workflow was terrible, they might be able to > offer good advice. > > On a positive, they were all very nice. > > Thank, > Tim H. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 10 > Date: Mon, 3 Feb 2014 09:43:06 -0800 > From: Dawn Bugge > Subject: [Histonet] Turn Around Time > To: "histonet@lists.utsouthwestern.edu" >? ? ? ??? > Message-ID: >? ? ? ??? Mo+igaK9Bz4BYX-Dnnc9xgw@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello Histonet > > I am just curious what the standard for Turn Around Time is for most labs. > I think a two day turn around time from the time the biopsy gets to the lab > to the time the pathologist signs out a case is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology > > > ------------------------------ > > Message: 11 > Date: Mon, 3 Feb 2014 12:49:14 -0500 > From: Tom McNemar > Subject: RE: [Histonet] Turn Around Time > To: 'Dawn Bugge' , >? ? ? ???"histonet@lists.utsouthwestern.edu" >? ? ? ??? > Message-ID: >? ? ? ??? > Content-Type: text/plain; charset="us-ascii" > > Our benchmark is 24 hour TAT for 80% of cases.? We provide same-day > service for recuts and most in-house special stains. > > Tom McNemar, HT(ASCP) > Histology Supervisor > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge > Sent: Monday, February 03, 2014 12:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Turn Around Time > > Hello Histonet > > I am just curious what the standard for Turn Around Time is for most labs. > I think a two day turn around time from the time the biopsy gets to the lab > to the time the pathologist signs out a case is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > > > > ------------------------------ > > Message: 12 > Date: Mon, 3 Feb 2014 17:56:47 +0000 > From: joelle weaver > Subject: RE: [Histonet] Turn Around Time > To: Tom McNemar , 'Dawn Bugge' >? ? ? ???,? ? "histonet@lists.utsouthwestern.edu" >? ? ? ??? > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > ditto. Only FISH is allowed to take longer > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: TMcNemar@lmhealth.org > > To: drbugge@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Mon, 3 Feb 2014 12:49:14 -0500 > > Subject: RE: [Histonet] Turn Around Time > > CC: > > > > Our benchmark is 24 hour TAT for 80% of cases.? We provide same-day > service for recuts and most in-house special stains. > > > > Tom McNemar, HT(ASCP) > > Histology Supervisor > > Licking Memorial Health Systems > > (740) 348-4163 > > (740) 348-4166 > > tmcnemar@lmhealth.org > > www.LMHealth.org > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge > > Sent: Monday, February 03, 2014 12:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Turn Around Time > > > > Hello Histonet > > > > I am just curious what the standard for Turn Around Time is for most > labs. > > I think a two day turn around time from the time the biopsy gets to the > lab > > to the time the pathologist signs out a case is pretty fast. > > > > Thanks for your input. > > -- > > Dawn R Bugge > > Seattle Histology > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 740-348-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 123, Issue 3 > **************************************** > ------------------------------ Message: 15 Date: Mon, 03 Feb 2014 17:27:38 -0500 From: Mike Thompson Subject: Re: [Histonet] Price for preparing IHC slides To: Michael Farmer Cc: histonet@lists.utsouthwestern.edu, Ann Specian Message-ID: Content-Type: text/plain; charset=utf-8 Read our motto below.? I've worked for the big IHC companies.? Now we will place everything at $10/slide w antibody. Instrument included. Michael O. Thompson Director of Sales Diagnostic BioSystems Phone: 1-888-896-3350 Mobile: 412-860-1288 Office Fax: 412-727-6080 "IHC Made Affordable" www.dbiosys.com Michael Farmer wrote: >This is a fascinating question, Ann - > >I've been studying this topic for the last couple of years in five countries. While I do not yet understand this complex phenomenon as well as I would wish to, these are my impressions about life here in the US. It is a ridiculous tale, but I will tell it to you... > >The smartest shoppers who have the biggest contracts (you can guess who those might be) are paying $5-10 for their highest-volume slides? - ER, PR, HER-2 and a few others - but more like? $10-15 for most of their menus. The smallest IHC customers think they are paying 20-something per slide, but they are actually paying $30-$40 per slide - and more in many cases. > >How this discrepancy? Two reasons: first, because the suppliers (I can't quite remember their names right now, please pardon my senior moment) are highly-skilled at making their price lists and service contracts as eye-glazingly complicated as possible. And second, because immutable human nature compels many mere mortals to underestimate their costs, particularly while they are still trying to rationalize a bad investment they made some time ago > >I'm pretty sure that American labs (excluding Canada, mind you) spent? $700-750m with the IHC companies in 2013. I think that between 28m and 32 million IHC slides were run last year in the US. If you want to slice those estimates down the middle you'll come up with maybe $24/slide, once every penny of waste, service, and sub-optimal operating procedures are truly accounted for. > >There you have one of the most useless averages you'll ever hear. There are plenty of contracts out there in vast middle America at every price point between $7 and $30 per slide. You pay what your volume earns you, unless of course you happen to be in the market for a tissue processor or primary stainer at the time you're haggling with the IHC companies. I'll remember their names if you give me another a minute. > >That's the way it was last year. WIth these new codes, lots of small labs will be priced out of the IHC business by summertime, and the big labs that remain will have negotiated prices within a narrower range. That'll be a fast-moving target. Since I didn't see you in the crowd at the funeral of 88342 last month, I am attaching below our chronicle of the event. > >I'm always happy to banter about this with anyone who thinks the topic is interesting. > >Sincerely, > >Michael Farmer >McEvoy & Farmer Pathology >www.mcevoyandfarmer-pathology.com >415-994-8852 > >"Those who seek the truth doubt those who find it" > - Andr?? Gide > > > > > > > > >On Feb 3, 2014, at 1:03 PM, Ann Specian wrote: > >> >> Can anyone tell me the average cost for preparing an IHC slide? >> thanks, Ann >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 123, Issue 4 **************************************** From suetp918 <@t> comcast.net Tue Feb 18 15:30:10 2014 From: suetp918 <@t> comcast.net (Sue) Date: Tue Feb 18 15:32:56 2014 Subject: [Histonet] Re: Lens vs sponges In-Reply-To: <1392756490.23876.YahooMailBasic@web161005.mail.bf1.yahoo.com> References: <1392756490.23876.YahooMailBasic@web161005.mail.bf1.yahoo.com> Message-ID: <27BECA68-7BA8-44E1-9608-B6C65C4481A2@comcast.net> We do not use sponges too much of a chance of floaters. Also aggregates of tissues end up in sponge and then is embedded Just my thoughts S Paturzo TJUH Sent from my iPhone > On Feb 18, 2014, at 3:48 PM, Sanjeet Dhirubhai wrote: > > > Hi Histo Folks, > > We are debating on what we should use during grossing either lens papers or sponges. > We are currently using sponges. And in the near future we will be moving to another Lab, basically a merger of 2 Histo depts. The other Lab uses lens papers. I need to know the pros and cons of Sponges verses lens paper. > with sponges there is carrier over of reagents. Is there any other or any major disadvantages of using sponges. Our reagent maintenance is done everyday and is standard. We do not go by the reagent management alerts from the Tissue Processor. > Help > > > Regards, > > > > Sanjeet Dhirubhai > > > > > > > > > > -------------------------------------------- > On Tue, 2/18/14, histonet-request@lists.utsouthwestern.edu wrote: > > Subject: Histonet Digest, Vol 123, Issue 4 > To: histonet@lists.utsouthwestern.edu > Received: Tuesday, February 18, 2014, 12:39 PM > > Send Histonet mailing list > submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' > to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more > specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Price for preparing IHC slides (Ann > Specian) > 2. Re: Price for preparing IHC slides > (Will Chappell) > 3. IHC cartilage (Victoria Rimkunas) > 4. Re: Turn Around Time (Jay Lundgren) > 5. dust-free coverslips - do they really > exist? (Connolly, Brett M) > 6. Re: Turn Around Time (Rene J Buesa) > 7. RE: Price for preparing IHC slides (Joe > W. Walker, Jr.) > 8. Re: Price for preparing IHC slides > (Michael Farmer) > 9. RE: Turn Around Time (Martha > Ward-Pathology) > 10. Re: Turn Around Time (Bob Richmond) > 11. RE: Turn Around Time (Joe W. Walker, Jr.) > 12. Re: Turn Around Time (Rene J Buesa) > 13. RE: How to De-stain trichrome (Tony Henwood > (SCHN)) > 14. Re: Histonet Digest, Vol 123, Issue 3 (Madeleine > Huey) > 15. Re: Price for preparing IHC slides (Mike > Thompson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 3 Feb 2014 13:03:28 -0500 (EST) > From: Ann Specian > Subject: [Histonet] Price for preparing IHC slides > To: histonet@lists.utsouthwestern.edu > Message-ID: <8D0EF215FBFEBF2-1E68-1A6E4@webmail-m233.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > > Can anyone tell me the average cost for preparing an IHC > slide? > thanks, Ann > > > ------------------------------ > > Message: 2 > Date: Mon, 3 Feb 2014 13:08:28 -0500 > From: Will Chappell > Subject: Re: [Histonet] Price for preparing IHC slides > To: Ann Specian > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: <77A77586-CF08-42B0-BABA-B972F803EA17@yahoo.com> > Content-Type: text/plain; > charset=us-ascii > > A lab I used in Southern California charged $35 per > stain for most antibodies. > > Sent from my iPhone > >> On Feb 3, 2014, at 1:03 PM, Ann Specian > wrote: >> >> >> Can anyone tell me the average cost for preparing an > IHC slide? >> thanks, Ann >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Mon, 3 Feb 2014 18:29:18 +0000 > From: Victoria Rimkunas > Subject: [Histonet] IHC cartilage > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <2661A06F49EDCF41ABFEE80C2727C2237204B5A6@MEP-MBX2.mack.local> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have experience or a protocol for IHC staining > on cartilage? > > > > ------------------------------ > > Message: 4 > Date: Mon, 3 Feb 2014 12:31:44 -0600 > From: Jay Lundgren > Subject: Re: [Histonet] Turn Around Time > To: joelle weaver > Cc: "histonet@lists.utsouthwestern.edu" > , > Tom McNemar > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Yesterday. > > Sincerely? > > Jay A. Lundgren, M.S., > HTL (ASCP) > > > On Mon, Feb 3, 2014 at 11:56 AM, joelle weaver wrote: > >> ditto. Only FISH is allowed to take longer >> >> >> >> >> Joelle Weaver MAOM, HTL (ASCP) QIHC >> >>> From: TMcNemar@lmhealth.org >>> To: drbugge@gmail.com; > histonet@lists.utsouthwestern.edu >>> Date: Mon, 3 Feb 2014 12:49:14 -0500 >>> Subject: RE: [Histonet] Turn Around Time >>> CC: >>> >>> Our benchmark is 24 hour TAT for 80% of > cases. We provide same-day >> service for recuts and most in-house special stains. >>> >>> Tom McNemar, HT(ASCP) >>> Histology Supervisor >>> Licking Memorial Health Systems >>> (740) 348-4163 >>> (740) 348-4166 >>> tmcnemar@lmhealth.org >>> www.LMHealth.org >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu > [mailto: >> histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dawn Bugge >>> Sent: Monday, February 03, 2014 12:43 PM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Turn Around Time >>> >>> Hello Histonet >>> >>> I am just curious what the standard for Turn > Around Time is for most >> labs. >>> I think a two day turn around time from the time > the biopsy gets to the >> lab >>> to the time the pathologist signs out a case is > pretty fast. >>> >>> Thanks for your input. >>> -- >>> Dawn R Bugge >>> Seattle Histology >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> This e-mail, including attachments, is intended > for the sole use of the >> individual and/or entity to whom it is addressed, and > contains information >> from Licking Memorial Health Systems which is > confidential or privileged. >> If you are not the intended recipient, nor authorized > to receive for the >> intended recipient, be aware that any disclosure, > copying, distribution or >> use of the contents of this e-mail and attachments is > prohibited. If you >> have received this in error, please advise the sender > by reply e-mail and >> delete the message immediately. You may also contact > the LMH Process >> Improvement Center at 740-348-4641. E-mail > transmissions cannot be >> guaranteed to be secure or error-free as information > could be intercepted, >> corrupted, lost, destroyed, arrive late or incomplete, > or contain viruses. >> The sender therefore does not accept liability for any > errors or omissions >> in the contents of this message, which arise as a > result of e-mail >> transmission. Thank you. >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ > > Message: 5 > Date: Mon, 3 Feb 2014 13:35:22 -0500 > From: "Connolly, Brett M" > Subject: [Histonet] dust-free coverslips - do they really > exist? > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > What's your favorite? It gets frustrating when I need > to capture low power images and there coverslips are dirty > right out of the box. > > Fisherfinest Premium isn't bad, but I am wondering if there > is something better??? > > Brett > > Brett M. Connolly, Ph.D. > Principal Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > > > > Notice: This e-mail message, together with any > attachments, contains > information of Merck & Co., Inc. (One Merck Drive, > Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact > information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be > confidential, > proprietary copyrighted and/or legally privileged. It is > intended solely > for the use of the individual or entity named on this > message. If you are > not the intended recipient, and have received this message > in error, > please notify us immediately by reply e-mail and then delete > it from > your system. > > > ------------------------------ > > Message: 6 > Date: Mon, 3 Feb 2014 10:38:14 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Turn Around Time > To: Tom McNemar , > 'Dawn Bugge' > , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1391452694.39779.YahooMailNeo@web120405.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > In the US the "standard" (or most frequent) TAT is 24 hours. > In other countries the TAT varies greatly, in extreme cases > up to 1 week for surgicals! > Ren? J. > > > ________________________________ > From: Tom McNemar > To: 'Dawn Bugge' ; > "histonet@lists.utsouthwestern.edu" > > > Sent: Monday, February 3, 2014 12:49 PM > Subject: RE: [Histonet] Turn Around Time > > > Our benchmark is 24 hour TAT for 80% of cases. We provide > same-day service for recuts and most in-house special > stains. > > Tom McNemar, HT(ASCP) > Histology Supervisor > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcnemar@lmhealth.org > www.LMHealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dawn Bugge > Sent: Monday, February 03, 2014 12:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Turn Around Time > > Hello Histonet > > I am just curious what the standard for Turn Around Time is > for most labs. > I think a two day turn around time from the time the biopsy > gets to the lab > to the time the pathologist signs out a case is pretty > fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail, including attachments, is intended for the sole > use of the individual and/or entity to whom it is addressed, > and contains information from Licking Memorial Health > Systems which is confidential or privileged. If you are not > the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, > distribution or use of the contents of this e-mail and > attachments is prohibited. If you have received this in > error, please advise the sender by reply e-mail and delete > the message immediately. You may also contact the LMH > Process Improvement Center at 740-348-4641. E-mail > transmissions cannot be guaranteed to be secure or > error-free as information could be intercepted, corrupted, > lost, destroyed, arrive late or incomplete, or contain > viruses. The sender therefore does not accept liability for > any errors or omissions in the contents of this message, > which arise as a result of e-mail transmission. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 7 > Date: Mon, 3 Feb 2014 18:43:49 +0000 > From: "Joe W. Walker, Jr." > Subject: RE: [Histonet] Price for preparing IHC slides > To: Ann Specian , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <3C2378778400AD448ADA6FD6BDB7CCCC181D2DCB@RRMBX03.rrmc.local> > Content-Type: text/plain; charset="iso-8859-1" > > I believe that this would be highly dependent on the > antibodies used, how often the antibodies are used, slide > costs, tech costs, overhead, etc. What would be > average for 1 institution might be above or below average > for another. > > What are you trying to determine, Ann? > > Joe W. Walker, Jr. MS, SCT(ASCP)CM > Manager of Anatomical Pathology, Microbiology and Reference > Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > P: 802.747.1790 F: 802.747.6525 > Email joewalker@rrmc.org > www.rrmc.org > > Our Vision: > To be the Best Community Healthcare System in New England > > Rutland Regional...Vermont's 1st Hospital to Achieve Both > ANCC Magnet Recognition? and the Governor's Award for > Performance Excellence > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Ann Specian > Sent: Monday, February 03, 2014 1:03 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Price for preparing IHC slides > > > Can anyone tell me the average cost for preparing an IHC > slide? > thanks, Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message (and any included attachments) is from Rutland > Regional Health Services and is intended only for the > addressee(s). The information contained herein may include > privileged or otherwise confidential information. > Unauthorized review, forwarding, printing, copying, > distributing, or using such information is strictly > prohibited and may be unlawful. If you received this message > in error, or have reason to believe you are not authorized > to receive it, please promptly delete this message and > notify the sender by e-mail. > > Thank You > > > > > ------------------------------ > > Message: 8 > Date: Mon, 3 Feb 2014 13:59:32 -0500 > From: Michael Farmer > Subject: Re: [Histonet] Price for preparing IHC slides > To: Ann Specian > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <35E9AD8D-1846-430D-BD59-71BB57DC7194@mcevoyandfarmer.com> > Content-Type: text/plain; charset="iso-8859-1" > > This is a fascinating question, Ann - > > I've been studying this topic for the last couple of years > in five countries. While I do not yet understand this > complex phenomenon as well as I would wish to, these are my > impressions about life here in the US. It is a ridiculous > tale, but I will tell it to you... > > The smartest shoppers who have the biggest contracts (you > can guess who those might be) are paying $5-10 for their > highest-volume slides - ER, PR, HER-2 and a few others > - but more like $10-15 for most of their menus. The > smallest IHC customers think they are paying 20-something > per slide, but they are actually paying $30-$40 per slide - > and more in many cases. > > How this discrepancy? Two reasons: first, because the > suppliers (I can't quite remember their names right now, > please pardon my senior moment) are highly-skilled at making > their price lists and service contracts as eye-glazingly > complicated as possible. And second, because immutable human > nature compels many mere mortals to underestimate their > costs, particularly while they are still trying to > rationalize a bad investment they made some time ago > > I'm pretty sure that American labs (excluding Canada, mind > you) spent $700-750m with the IHC companies in 2013. I > think that between 28m and 32 million IHC slides were run > last year in the US. If you want to slice those estimates > down the middle you'll come up with maybe $24/slide, once > every penny of waste, service, and sub-optimal operating > procedures are truly accounted for. > > There you have one of the most useless averages you'll ever > hear. There are plenty of contracts out there in vast middle > America at every price point between $7 and $30 per slide. > You pay what your volume earns you, unless of course you > happen to be in the market for a tissue processor or primary > stainer at the time you're haggling with the IHC companies. > I'll remember their names if you give me another a minute. > > That's the way it was last year. WIth these new codes, lots > of small labs will be priced out of the IHC business by > summertime, and the big labs that remain will have > negotiated prices within a narrower range. That'll be a > fast-moving target. Since I didn't see you in the crowd at > the funeral of 88342 last month, I am attaching below our > chronicle of the event. > > I'm always happy to banter about this with anyone who thinks > the topic is interesting. > > Sincerely, > > Michael Farmer > McEvoy & Farmer Pathology > www.mcevoyandfarmer-pathology.com > 415-994-8852 > > "Those who seek the truth doubt those who find it" > - Andr? Gide > > -------------- next part -------------- > > > > > > >> On Feb 3, 2014, at 1:03 PM, Ann Specian wrote: >> >> >> Can anyone tell me the average cost for preparing an > IHC slide? >> thanks, Ann >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 9 > Date: Mon, 3 Feb 2014 19:05:47 +0000 > From: Martha Ward-Pathology > Subject: RE: [Histonet] Turn Around Time > To: Dawn Bugge , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > While I recognize the need for a quick result in some cases, > I also subscribe to the theory that "speed > kills". I'm not sure that these quick TATs > are always medically necessary, but rather more of a > convenience. However that is another discussion > altogether. That said, our institution shoots for the > 80% in 24 hours as well. > > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 > p 336.716.2109 \ f 336.716.5890 > mward@wakehealth.edu > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dawn Bugge > Sent: Monday, February 03, 2014 12:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Turn Around Time > > Hello Histonet > > I am just curious what the standard for Turn Around Time is > for most labs. > I think a two day turn around time from the time the biopsy > gets to the lab to the time the pathologist signs out a case > is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Mon, 3 Feb 2014 14:50:45 -0500 > From: Bob Richmond > Subject: [Histonet] Re: Turn Around Time > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Dawn R. Bugge at Seattle WA Histology asks: > >>> I am just curious what the standard for Turn Around > Time is for most > labs. I think a two day turn around time from the time the > biopsy gets to > the lab to the time the pathologist signs out a case is > pretty fast.<< > > Normally I'd receive the specimen on Monday and gross it, > have it processed > overnight, get the slides some time Tuesday morning, and > sign the case out > well before close of business on Tuesday. > > Exceptions are when the specimen needs to fix overnight > before I gross it > (bowel resection) or after I gross it (fatty breast tissue, > amputation > specimens, autopsies), or when a specimen of more than > minimal size (or > received after 1400) requires decalcification. What I say > goes. > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Message: 11 > Date: Mon, 3 Feb 2014 19:54:21 +0000 > From: "Joe W. Walker, Jr." > Subject: RE: [Histonet] Turn Around Time > To: Martha Ward-Pathology , > Dawn Bugge > , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <3C2378778400AD448ADA6FD6BDB7CCCC181D4260@RRMBX03.rrmc.local> > Content-Type: text/plain; charset="iso-8859-1" > > I agree with you, Martha. That is why we look at it > from an average of all cases for the month. We > definitely encounter cases that are difficult and require > more time with our pathologists. We track the TAT for > statistical purposes only. The CAP (for those who are > CAP accredited) has dropped this from their checklist for > surgical cases. It seems to only really apply to > autopsy cases now but these has a much different TAT > target. > > Joe W. Walker, Jr. MS, SCT(ASCP)CM > Manager of Anatomical Pathology, Microbiology and Reference > Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > P: 802.747.1790 F: 802.747.6525 > Email joewalker@rrmc.org > www.rrmc.org > > Our Vision: > To be the Best Community Healthcare System in New England > > Rutland Regional...Vermont's 1st Hospital to Achieve Both > ANCC Magnet Recognition? and the Governor's Award for > Performance Excellence > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Martha Ward-Pathology > Sent: Monday, February 03, 2014 2:06 PM > To: Dawn Bugge; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Turn Around Time > > While I recognize the need for a quick result in some cases, > I also subscribe to the theory that "speed > kills". I'm not sure that these quick TATs > are always medically necessary, but rather more of a > convenience. However that is another discussion > altogether. That said, our institution shoots for the > 80% in 24 hours as well. > > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC > 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dawn Bugge > Sent: Monday, February 03, 2014 12:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Turn Around Time > > Hello Histonet > > I am just curious what the standard for Turn Around Time is > for most labs. > I think a two day turn around time from the time the biopsy > gets to the lab to the time the pathologist signs out a case > is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message (and any included attachments) is from Rutland > Regional Health Services and is intended only for the > addressee(s). The information contained herein may include > privileged or otherwise confidential information. > Unauthorized review, forwarding, printing, copying, > distributing, or using such information is strictly > prohibited and may be unlawful. If you received this message > in error, or have reason to believe you are not authorized > to receive it, please promptly delete this message and > notify the sender by e-mail. > > Thank You > > > > > ------------------------------ > > Message: 12 > Date: Mon, 3 Feb 2014 13:37:49 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Turn Around Time > To: Martha Ward-Pathology , > Dawn Bugge > , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1391463469.21418.YahooMailNeo@web120406.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Martha: > So, do you mean that you are not satisfied with the quality > of your slides? > If you are, I just do not understand your concerns about a > 24 h TAT! > The fundamental issue is to develop adequate protocols > assuring quality as well as a convenient (24 h) TAT. > Just my 3 cents (after inflation!) > Ren? J. > > > ________________________________ > From: Martha Ward-Pathology > To: Dawn Bugge ; > "histonet@lists.utsouthwestern.edu" > > > Sent: Monday, February 3, 2014 2:05 PM > Subject: RE: [Histonet] Turn Around Time > > > While I recognize the need for a quick result in some cases, > I also subscribe to the theory that "speed kills". I'm not > sure that these quick TATs are always medically necessary, > but rather more of a convenience. However that is > another discussion altogether. That said, our institution > shoots for the 80% in 24 hours as well. > > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 > p 336.716.2109 \ f 336.716.5890 > mward@wakehealth.edu > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dawn Bugge > Sent: Monday, February 03, 2014 12:43 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Turn Around Time > > Hello Histonet > > I am just curious what the standard for Turn Around Time is > for most labs. > I think a two day turn around time from the time the biopsy > gets to the lab to the time the pathologist signs out a case > is pretty fast. > > Thanks for your input. > -- > Dawn R Bugge > Seattle Histology > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 13 > Date: Mon, 3 Feb 2014 21:58:51 +0000 > From: "Tony Henwood (SCHN)" > Subject: RE: [Histonet] How to De-stain trichrome > To: "'Hans B Snyder'" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00AD9EE@xmdb04.nch.kids> > Content-Type: text/plain; charset="us-ascii" > > Hi Hans, > > It is possible, though it will depend on what you want to do > to the slides afterword. > The problem arises from the use of phosphotungstic or > phosphmolybdic acid which is used for differentiation and > mordanting in the trichrome stains like Masson's. > These are very difficult to reverse (anyone trying to > restain PAP smears might know the problem). This may cause > problems. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), > FFSc(RCPA) > Laboratory Manager & Senior Scientist, the Children's > Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western > Sydney > > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Hans B Snyder > Sent: Tuesday, 4 February 2014 1:54 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How to De-stain trichrome > > Does anyone know how to destain Massons trichrome? Is it > possible? > > Thank you > > Histologistics > Hans B Snyder > 508.308.7800 > Hans@histologistics.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are > confidential and intended solely for the use of the > individual or entity to whom they are addressed. If you are > not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are > those of the individual sender, and are not necessarily the > views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been > virus scanned and although no computer viruses were > detected, The Sydney Childrens Hospital's Network accepts no > liability for any consequential damage resulting from email > containing computer viruses. > ********************************************************************************* > > > > ------------------------------ > > Message: 14 > Date: Mon, 3 Feb 2014 14:26:00 -0800 > From: Madeleine Huey > Subject: [Histonet] Re: Histonet Digest, Vol 123, Issue 3 > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Dawn, > Our TAT is 94% with 24 hours & 98% within 48 hours (~ 6% > complicated cases > need more IPOX/Molecular Study/FACS & etc.). > Madeleine > Superviser - Pathology (IPOX & Histology) > madeleine_h@elcaminohospital.org > > > From: histonet-bounces@lists.utsouthwestern.edu > [mailto: > histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dawn Bugge >> Sent: Monday, February 03, 2014 12:43 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Turn Around Time >> >> Hello Histonet >> >> I am just curious what the standard for Turn Around > Time is for most labs. >> I think a two day turn around time from the time the > biopsy gets to the > lab >> to the time the pathologist signs out a case is pretty > fast. >> >> Thanks for your input. >> -- >> Dawn R Bugge >> Seattle Histology > > > On Mon, Feb 3, 2014 at 10:00 AM, > wrote: > >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, > visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body > 'help' to >> histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is > more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. Re: Gross lab seniors (WILLIAM > DESALVO) >> 2. Re: protocols to fix insect wings that > are quite hirsute (Damien) >> 3. RE: Ventana LEAN (Tim Higgins) >> 4. RE: RE: Ventana LEAN (Rathborne, Toni) >> 5. How to De-stain trichrome (Hans B > Snyder) >> 6. Histotech position FT West Palm Beach > (nicole@dlcjax.com) >> 7. anyone need a glass cover slipper > (Curt) >> 8. On-call position for Histology > Assistant in Phoenix AZ (Jill Cox) >> 9. RE: RE: Ventana LEAN (Eytalis, Robert > A) >> 10. Turn Around Time (Dawn Bugge) >> 11. RE: Turn Around Time (Tom > McNemar) >> 12. RE: Turn Around Time (joelle > weaver) >> >> >> > ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Sun, 2 Feb 2014 18:34:15 +0000 >> From: WILLIAM DESALVO >> Subject: Re: [Histonet] Gross lab seniors >> To: E. Wayne Johnson ??? >> Cc: histonet , > " Vickroy, Jim " >> >> Message-ID: >> Content-Type: text/plain; charset="utf-8" >> >> Absolutely the Face velocity changes with the work area > set-up. This is >> why I like to make sure the air flow away is > maintained at a minimum. Most >> grossing stations have large working area and often the > flow away from the >> grosser is not checked, just the vent opening draw. > Most important is to >> set up a process and then regularly check. >> >> >> >> >> >> >> Sent from Windows Mail >> >> >> >> >> >> From: E. Wayne Johnson ????????? >> Sent: ???Saturday???, ???February??? > ???1???, ???2014 > ???3???:???12??? ???PM >> To: WILLIAM DESALVO >> Cc: Vickroy, Jim, histonet >> >> >> >> >> >> Face velocity is simply the airflow rate in CFM divided > by the area of >> the hood opening in square feet. >> >> A smaller opening at the same flow rate gives a higher > face velocity. >> >> Titanium tetrachloride in a small plastic squeeze > bottle can be used to >> generate "smoke". >> >> >>> On 3:59 AM, WILLIAM DESALVO wrote: >>> We use a company called C-Scan Technologies, > Phoenix, AZ. The way they >> test all our gross dissection stations is by testing > for directional or >> smoke containment and face velocity. We also check th > they external pathway >> is clear and if the unit has a filtering system, the > filters are changed >> regularly. The air flow measurement is Feet per minute > (FPM) for face >> velocity and includes width, height, depth and total > square ft for the >> working area. They exhaust flow in CFM. Face velocity > minimum requirement >> is 100 fpm, exhaust flow requirement is>500 cfm. > Face velocity fluctuates >> depending on the room and the air exchange rate for the > area. I have always >> felt the face velocity is most important to gross > dissection personnel. >> There needs to be adequate draw away from the employee, > no matter the >> physical conditions of the room. >>> >>> William DeSalvo, BS HTL(ASCP) >>> Production Manager-Anatomic Pathology >>> Chair, NSH Quality Management Committee >>> Owner/Consultant, Collaborative Advantage > Consulting >>> >>> >>> >>>> From: Vickroy.Jim@mhsil.com >>>> To: histonet@lists.utsouthwestern.edu >>>> Date: Fri, 31 Jan 2014 12:46:08 -0600 >>>> Subject: [Histonet] Gross lab seniors >>>> >>>> >>>> We have several gross lab senior grossing > stations that are vented >> outside. Our engineering asked today whether the > airflow should be checked >> yearly like other exhaust > hoods. Problem is there is not a door like >> other hoods of course and how would you measure the > airflow? Recommended >> airflow is 500cfm however clearly the airflow at the > working surface is not >> anything close to that. I wondered how > anybody else monitors the gross >> lab seniors or do they at all. CAP > used to ask about documentation for >> checking hoods however I can't recall them ever > checking on grossing >> stations. We change filters annually only > since they are vented outside. >>>> >>>> Jim >>>> >>>> James Vickroy BS, HT(ASCP) >>>> >>>> Surgical and Autopsy Pathology Technical > Supervisor >>>> Memorial Medical Center >>>> 217-788-4046 >>>> >>>> >>>> ________________________________ >>>> This message (including any attachments) > contains confidential >> information intended for a specific individual and > purpose, and is >> protected by law. If you are not the intended > recipient, you should delete >> this message. Any disclosure, copying, or distribution > of this message, or >> the taking of any action based on it, is strictly > prohibited. >>>> > _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> >> >> ------------------------------ >> >> Message: 2 >> Date: Mon, 3 Feb 2014 05:40:33 -0500 >> From: Damien >> Subject: Re: [Histonet] protocols to fix insect wings > that are quite >> hirsute >> To: Jack Ratliff >> Cc: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> > > vq+Qrw55qKhWdQ@mail.gmail.com> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hi Jorge, >> >> I'd be happy to help you if I can. Please feel free to > message me. >> >> Best, >> Damien L >> >> >> On Sun, Feb 2, 2014 at 10:45 AM, Jack Ratliff >> wrote: >> >>> This sounds like a question for the insect > histology expert, Damien >>> Laudier! He monitors this site so I am sure he > will respond to you >>> privately. :) >>> >>> Jack >>> >>>> On Feb 1, 2014, at 5:00 PM, "Jorge A. > Santiago-Blay" < >>> blayjorge@gmail.com> > wrote: >>>> >>>> Hello: >>>> >>>> Can someone point me on the direction of > protocols to fix insect wings >>> that >>>> are quite hirsute. I would like to increase > the likelihood of the >>>> preservative actually going through the > carpet of setae to actually fix >>> the >>>> interior of the wing. Thanks for any help. >>>> >>>> Sincerely, >>>> >>>> Jorge >>>> >>>> Jorge A. Santiago-Blay, PhD >>>> blaypublishers.com >>>> http://blayjorge.wordpress.com/ >>>> http://paleobiology.si.edu/staff/individuals/santiagoblay.html >>>> > _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> -- >> Damien Laudier >> Laudier Histology >> www.LaudierHistology.com >> >> >> ------------------------------ >> >> Message: 3 >> Date: Mon, 3 Feb 2014 07:58:51 -0600 >> From: Tim Higgins >> Subject: [Histonet] RE: Ventana LEAN >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> They come in, give their opinions on how to make your > workflow better. I >> didn't get much from it, we didn't make any changes, > more of a selling tool >> in my opion. I guess if your workflow was > terrible, they might be able to >> offer good advice. >> >> On a positive, they were all very nice. >> >> Thank, >> Tim H. >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Mon, 3 Feb 2014 14:32:59 +0000 >> From: "Rathborne, Toni" >> Subject: RE: [Histonet] RE: Ventana LEAN >> To: "'Tim Higgins'" , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> 3AD061FE740D464FAC7BF6B5CFB75707A95B53C7@SMCMAIL01.somerset-healthcare.com >>> >> >> Content-Type: text/plain; charset="us-ascii" >> >> Another benefit of someone coming in from the outside > (Leica did the same >> for us), is that since this is what these people do > every day, management >> is more likely to listen to their suggestions. If it's > the same as yours >> then that would reinforce your current plans. If it's > different than what >> you had in mind, they will generally try to help with > what you want to do. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu > [mailto: >> histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Tim Higgins >> Sent: Monday, February 03, 2014 8:59 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: Ventana LEAN >> >> They come in, give their opinions on how to make your > workflow better. I >> didn't get much from it, we didn't make any changes, > more of a selling tool >> in my opion. I guess if your workflow was > terrible, they might be able to >> offer good advice. >> >> On a positive, they were all very nice. >> >> Thank, >> Tim H. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Mon, 3 Feb 2014 09:53:45 -0500 >> From: Hans B Snyder >> Subject: [Histonet] How to De-stain trichrome >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> Content-Type: text/plain; > charset=us-ascii >> >> Does anyone know how to destain Massons trichrome? Is > it possible? >> >> Thank you >> >> Histologistics >> Hans B Snyder >> 508.308.7800 >> Hans@histologistics.com >> >> >> >> ------------------------------ >> >> Message: 6 >> Date: Mon, 3 Feb 2014 15:19:07 +0000 (UTC) >> From: nicole@dlcjax.com >> Subject: [Histonet] Histotech position FT West Palm > Beach >> To: histonet@lists.utsouthwestern.edu >> Message-ID: > <1940731504.67335.1391440747343.JavaMail.mail@webmail17> >> Content-Type: text/plain; charset="UTF-8" >> >> >> Posting > for I/MD Path labs West Palm > Beach, Florida Looking for >> full time Histotech. > Dermpath lab. Please contact > Dr. Morales. >> 1(561)653-8005 >> >> >> ------------------------------ >> >> Message: 7 >> Date: Mon, 3 Feb 2014 17:06:56 +0000 >> From: Curt >> Subject: [Histonet] anyone need a glass cover slipper >> To: "Histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> >> <9C8F910F72893643B3C3793C3D67132B01440853@PATHOLOGYSERVER.pathologyarts.local >>> >> >> Content-Type: text/plain; charset="us-ascii" >> >> We have on old Hacker glass cover slipper, I think > it's a Hacker.... The >> name plate on the front says Meisei but I'm told it's a > Hacker. Anyway, the >> model number: RCM-3660 if that's helpful. >> >> I'm not looking to make a bunch of money, so many > people here have been >> helpful over the years from knowledge and advice to > helping me out with >> control tissue. >> >> If someone needs one, I'd be willing to help out a bit, > just need to cover >> the expense of shipping and packing. >> >> Let me know, I can snap a picture if desired. We > recently had is serviced >> and it's said to be working fine by the technician. >> >> >> Curt >> >> >> >> ------------------------------ >> >> Message: 8 >> Date: Mon, 3 Feb 2014 08:58:56 -0800 (PST) >> From: Jill Cox >> Subject: [Histonet] On-call position for Histology > Assistant in >> Phoenix AZ >> To: "Histonet@Lists. Edu" >> Message-ID: >> <1391446736.89252.YahooMailBasic@web160701.mail.bf1.yahoo.com> >> Content-Type: text/plain; charset=us-ascii >> >> Hi Netters, >> We are looking for an on-call histology assistant to > cover for vacations >> and sick leave, already have a few days in March/April. > We are a small >> Dermatology lab in NW Peoria AZ, great working > conditions and people. Very >> nice place to work. Hours are from 7:30-4:00PM when > needed. Email me for >> more details. >> Thank you, Jill >> >> Arrowhead Dermatology >> >> >> >> >> ------------------------------ >> >> Message: 9 >> Date: Mon, 3 Feb 2014 17:00:28 +0000 >> From: "Eytalis, Robert A" >> Subject: RE: [Histonet] RE: Ventana LEAN >> To: "Rathborne, Toni" , > "'Tim >> Higgins'" > , > " >> histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> < >> F3024E541CBF9049BD4A805E1646204A6F8CE5A0@RHCMBX02.RiversideHealthCare.net> >> >> Content-Type: text/plain; charset="us-ascii" >> >> Depends on if your management is skeptical of vendors. > Mine automatically >> assume that they are marketing to us. >> >> Robert A. Eytalis >> Laboratory Manager >> robert-eytalis@riversidehealthcare.net >> Phone: (815) 935-7256 ext. 5186 >> > (815) 935-7535 >> Fax (815) > 935-7068 >> >> Riverside Medical Center >> 350 N. Wall Street - Kankakee, IL 60901 >> >> >> >> http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.riversidemc.net%2f > | >> http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.&URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC >> >> >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu > [ >> histonet-bounces@lists.utsouthwestern.edu] > on behalf of Rathborne, Toni [ >> trathborne@somerset-healthcare.com] >> Sent: Monday, February 03, 2014 8:32 AM >> To: 'Tim Higgins'; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] RE: Ventana LEAN >> >> Another benefit of someone coming in from the outside > (Leica did the same >> for us), is that since this is what these people do > every day, management >> is more likely to listen to their suggestions. If it's > the same as yours >> then that would reinforce your current plans. If it's > different than what >> you had in mind, they will generally try to help with > what you want to do. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu > [mailto: >> histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Tim Higgins >> Sent: Monday, February 03, 2014 8:59 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] RE: Ventana LEAN >> >> They come in, give their opinions on how to make your > workflow better. I >> didn't get much from it, we didn't make any changes, > more of a selling tool >> in my opion. I guess if your workflow was > terrible, they might be able to >> offer good advice. >> >> On a positive, they were all very nice. >> >> Thank, >> Tim H. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Message: 10 >> Date: Mon, 3 Feb 2014 09:43:06 -0800 >> From: Dawn Bugge >> Subject: [Histonet] Turn Around Time >> To: "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> > > Mo+igaK9Bz4BYX-Dnnc9xgw@mail.gmail.com> >> Content-Type: text/plain; charset=ISO-8859-1 >> >> Hello Histonet >> >> I am just curious what the standard for Turn Around > Time is for most labs. >> I think a two day turn around time from the time the > biopsy gets to the lab >> to the time the pathologist signs out a case is pretty > fast. >> >> Thanks for your input. >> -- >> Dawn R Bugge >> Seattle Histology >> >> >> ------------------------------ >> >> Message: 11 >> Date: Mon, 3 Feb 2014 12:49:14 -0500 >> From: Tom McNemar >> Subject: RE: [Histonet] Turn Around Time >> To: 'Dawn Bugge' , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> > >> Content-Type: text/plain; charset="us-ascii" >> >> Our benchmark is 24 hour TAT for 80% of cases. We > provide same-day >> service for recuts and most in-house special stains. >> >> Tom McNemar, HT(ASCP) >> Histology Supervisor >> Licking Memorial Health Systems >> (740) 348-4163 >> (740) 348-4166 >> tmcnemar@lmhealth.org >> www.LMHealth.org >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu > [mailto: >> histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dawn Bugge >> Sent: Monday, February 03, 2014 12:43 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Turn Around Time >> >> Hello Histonet >> >> I am just curious what the standard for Turn Around > Time is for most labs. >> I think a two day turn around time from the time the > biopsy gets to the lab >> to the time the pathologist signs out a case is pretty > fast. >> >> Thanks for your input. >> -- >> Dawn R Bugge >> Seattle Histology >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This e-mail, including attachments, is intended for the > sole use of the >> individual and/or entity to whom it is addressed, and > contains information >> from Licking Memorial Health Systems which is > confidential or privileged. >> If you are not the intended recipient, nor authorized > to receive for the >> intended recipient, be aware that any disclosure, > copying, distribution or >> use of the contents of this e-mail and attachments is > prohibited. If you >> have received this in error, please advise the sender > by reply e-mail and >> delete the message immediately. You may also contact > the LMH Process >> Improvement Center at 740-348-4641. E-mail > transmissions cannot be >> guaranteed to be secure or error-free as information > could be intercepted, >> corrupted, lost, destroyed, arrive late or incomplete, > or contain viruses. >> The sender therefore does not accept liability for any > errors or omissions >> in the contents of this message, which arise as a > result of e-mail >> transmission. Thank you. >> >> >> >> ------------------------------ >> >> Message: 12 >> Date: Mon, 3 Feb 2014 17:56:47 +0000 >> From: joelle weaver >> Subject: RE: [Histonet] Turn Around Time >> To: Tom McNemar , > 'Dawn Bugge' >> , > "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> ditto. Only FISH is allowed to take longer >> >> >> >> >> Joelle Weaver MAOM, HTL (ASCP) QIHC >> >>> From: TMcNemar@lmhealth.org >>> To: drbugge@gmail.com; > histonet@lists.utsouthwestern.edu >>> Date: Mon, 3 Feb 2014 12:49:14 -0500 >>> Subject: RE: [Histonet] Turn Around Time >>> CC: >>> >>> Our benchmark is 24 hour TAT for 80% of > cases. We provide same-day >> service for recuts and most in-house special stains. >>> >>> Tom McNemar, HT(ASCP) >>> Histology Supervisor >>> Licking Memorial Health Systems >>> (740) 348-4163 >>> (740) 348-4166 >>> tmcnemar@lmhealth.org >>> www.LMHealth.org >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu > [mailto: >> histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dawn Bugge >>> Sent: Monday, February 03, 2014 12:43 PM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Turn Around Time >>> >>> Hello Histonet >>> >>> I am just curious what the standard for Turn > Around Time is for most >> labs. >>> I think a two day turn around time from the time > the biopsy gets to the >> lab >>> to the time the pathologist signs out a case is > pretty fast. >>> >>> Thanks for your input. >>> -- >>> Dawn R Bugge >>> Seattle Histology >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> This e-mail, including attachments, is intended > for the sole use of the >> individual and/or entity to whom it is addressed, and > contains information >> from Licking Memorial Health Systems which is > confidential or privileged. >> If you are not the intended recipient, nor authorized > to receive for the >> intended recipient, be aware that any disclosure, > copying, distribution or >> use of the contents of this e-mail and attachments is > prohibited. If you >> have received this in error, please advise the sender > by reply e-mail and >> delete the message immediately. You may also contact > the LMH Process >> Improvement Center at 740-348-4641. E-mail > transmissions cannot be >> guaranteed to be secure or error-free as information > could be intercepted, >> corrupted, lost, destroyed, arrive late or incomplete, > or contain viruses. >> The sender therefore does not accept liability for any > errors or omissions >> in the contents of this message, which arise as a > result of e-mail >> transmission. Thank you. >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 123, Issue 3 >> **************************************** >> > > > ------------------------------ > > Message: 15 > Date: Mon, 03 Feb 2014 17:27:38 -0500 > From: Mike Thompson > Subject: Re: [Histonet] Price for preparing IHC slides > To: Michael Farmer > Cc: histonet@lists.utsouthwestern.edu, > Ann Specian > Message-ID: > Content-Type: text/plain; charset=utf-8 > > Read our motto below. I've worked for the big IHC > companies. Now we will place everything at $10/slide w > antibody. Instrument included. > > > Michael O. Thompson > Director of Sales > Diagnostic BioSystems > Phone: 1-888-896-3350 > Mobile: 412-860-1288 > Office Fax: 412-727-6080 > > "IHC Made Affordable" > www.dbiosys.com > > Michael Farmer > wrote: > >> This is a fascinating question, Ann - >> >> I've been studying this topic for the last couple of > years in five countries. While I do not yet understand this > complex phenomenon as well as I would wish to, these are my > impressions about life here in the US. It is a ridiculous > tale, but I will tell it to you... >> >> The smartest shoppers who have the biggest contracts > (you can guess who those might be) are paying $5-10 for > their highest-volume slides - ER, PR, HER-2 and a few > others - but more like $10-15 for most of their menus. > The smallest IHC customers think they are paying > 20-something per slide, but they are actually paying $30-$40 > per slide - and more in many cases. >> >> How this discrepancy? Two reasons: first, because the > suppliers (I can't quite remember their names right now, > please pardon my senior moment) are highly-skilled at making > their price lists and service contracts as eye-glazingly > complicated as possible. And second, because immutable human > nature compels many mere mortals to underestimate their > costs, particularly while they are still trying to > rationalize a bad investment they made some time ago >> >> I'm pretty sure that American labs (excluding Canada, > mind you) spent $700-750m with the IHC companies in > 2013. I think that between 28m and 32 million IHC slides > were run last year in the US. If you want to slice those > estimates down the middle you'll come up with maybe > $24/slide, once every penny of waste, service, and > sub-optimal operating procedures are truly accounted for. >> >> There you have one of the most useless averages you'll > ever hear. There are plenty of contracts out there in vast > middle America at every price point between $7 and $30 per > slide. You pay what your volume earns you, unless of course > you happen to be in the market for a tissue processor or > primary stainer at the time you're haggling with the IHC > companies. I'll remember their names if you give me another > a minute. >> >> That's the way it was last year. WIth these new codes, > lots of small labs will be priced out of the IHC business by > summertime, and the big labs that remain will have > negotiated prices within a narrower range. That'll be a > fast-moving target. Since I didn't see you in the crowd at > the funeral of 88342 last month, I am attaching below our > chronicle of the event. >> >> I'm always happy to banter about this with anyone who > thinks the topic is interesting. >> >> Sincerely, >> >> Michael Farmer >> McEvoy & Farmer Pathology >> www.mcevoyandfarmer-pathology.com >> 415-994-8852 >> >> "Those who seek the truth doubt those who find it" >> - Andr?? Gide >> >> >> >> >> >> >> >> >>> On Feb 3, 2014, at 1:03 PM, Ann Specian wrote: >>> >>> >>> Can anyone tell me the average cost for preparing > an IHC slide? >>> thanks, Ann >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 123, Issue 4 > **************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue Feb 18 15:47:32 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Feb 18 15:47:43 2014 Subject: [Histonet] re: Neutralization of AZF Message-ID: <9F3CFEE76E51B64991C7485270890B4049832ED6@EX5.lj.gnf.org> Hi Jim, Do you know what exactly you are neutralizing? Does AZF stand for alcoholic Zinc Formalin? One of the best sources for information regarding zinc formalin products is Anatech, Ltd - www.anatechltdusa.com Call them and ask about their technique (and additional references) regarding neutralization. They are always happy to help us with these issues. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From ahc53 <@t> georgetown.edu Tue Feb 18 16:01:20 2014 From: ahc53 <@t> georgetown.edu (Anna Coffey) Date: Tue Feb 18 16:01:24 2014 Subject: [Histonet] Lens vs sponges Message-ID: We use sponges pretty regularly in our lab, though have had trouble with them sticking to particularly small and fragile samples. We have switched to using Telfa for the more fragile/delicate samples (works well, although the Telfa is sometimes difficult to cut down to the right size for the cassettes), though we have not tried lens paper before. For most samples, the sponges work well. I just make sure to dip them in the fixative before placing the tissue on them to avoid potential artifacts in the sections. -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu From argonautro <@t> yahoo.com Tue Feb 18 16:59:54 2014 From: argonautro <@t> yahoo.com (Pirici Daniel) Date: Tue Feb 18 17:00:02 2014 Subject: [Histonet] Corpora amylacea are destroyed after antigen retrieval? Message-ID: <1392764394.97139.YahooMailNeo@web121204.mail.ne1.yahoo.com> Has anyone observed corpora amylacea on brain tissue being destroyed after antigen retrieval (for example microwaving in citrate buffer)? We are experiencing this problem, as we are interested to study these structures, and we are not sure if it is a common artifact when preparing slides for IHC... ? Thanks so much for your time! Daniel ________________________________________________________________________________________________ Pirici Nicolae Daniel, MD, PhD Department of Histology University of Medicine and Pharmacy Craiova Petru Rares Street 2, 200349 Craiova, Dolj Romania From Finlay.Finlay <@t> glasgow.ac.uk Wed Feb 19 05:31:15 2014 From: Finlay.Finlay <@t> glasgow.ac.uk (Finlay Finlay) Date: Wed Feb 19 05:31:29 2014 Subject: [Histonet] wearable formalin monitors Message-ID: Hello Just wondering what everyone's experience is with different wearable formalin monitors. I'm looking at the AirChek ones from Leica at the moment. Thank you Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay@glasgow.ac.uk The University of Glasgow Charity Number SC004401 From Timothy.Morken <@t> ucsfmedctr.org Wed Feb 19 10:26:53 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Feb 19 10:27:07 2014 Subject: [Histonet] RE: wearable formalin monitors In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF3675884D@ex07.net.ucsf.edu> Our formalin and xylene badges are from Advanced Chemical Sensors, Boca Raton, FL. Our Saftey group supplies these to use for monitoring. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Finlay Finlay Sent: Wednesday, February 19, 2014 3:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] wearable formalin monitors Hello Just wondering what everyone's experience is with different wearable formalin monitors. I'm looking at the AirChek ones from Leica at the moment. Thank you Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay@glasgow.ac.uk The University of Glasgow Charity Number SC004401 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Feb 19 11:27:52 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Feb 19 11:27:58 2014 Subject: [Histonet] aqueous mounting medium Message-ID: Does anyone have a good "recipe" for aqueous mounting medium? Thanks, Jennifer From bbrinegarhtl <@t> gmail.com Wed Feb 19 11:38:28 2014 From: bbrinegarhtl <@t> gmail.com (Beth Brinegar) Date: Wed Feb 19 11:38:33 2014 Subject: [Histonet] To those attending Tri-State in Rochester MN Message-ID: Just giving a heads up to those needing hotels for the TriState Symposium 4/30-5/2 that the Super 8 is located just 1.1 miles down S Broadway from the Double Tree Hotel (where the symposium is being held) and is only $50.49 a night (if booked early) instead of $132.00 a night (at the Double Tree). Just thought I'd give you all a heads up! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 From hans <@t> histologistics.com Wed Feb 19 12:11:41 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Wed Feb 19 12:11:51 2014 Subject: [Histonet] aqueous mounting medium In-Reply-To: References: Message-ID: Hello, I have one for ORO but not for IF. If someone has one for IF, I would be interested also. Thank you Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com > On Feb 19, 2014, at 12:27, Jennifer MacDonald wrote: > > Does anyone have a good "recipe" for aqueous mounting medium? > Thanks, > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> gladstone.ucsf.edu Wed Feb 19 12:22:58 2014 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Wed Feb 19 12:23:03 2014 Subject: [Histonet] aqueous mounting medium In-Reply-To: References: Message-ID: I use prolong gold from life tech (formerly molecular probes and now part of the world dominating thermofisher) without Dapi as I do that separately, for IF and aquaslip from American master tech for non IF aqueous mounting Good luck! Caroline Caroline Miller Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 > On Feb 19, 2014, at 10:11 AM, Hans B Snyder wrote: > > Hello, > > I have one for ORO but not for IF. If someone has one for IF, I would be interested also. > > Thank you > > Histologistics > Hans B Snyder > 508.308.7800 > Hans@histologistics.com > >> On Feb 19, 2014, at 12:27, Jennifer MacDonald wrote: >> >> Does anyone have a good "recipe" for aqueous mounting medium? >> Thanks, >> Jennifer >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TanyaAbbott <@t> catholichealth.net Wed Feb 19 12:24:50 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Feb 19 12:24:58 2014 Subject: [Histonet] Billing Message-ID: <852F7D2C14FB464D80E182B15DB138AF30637E73@CHIEX006.CHI.catholichealth.net> Does anyone bill for a "technical component" for send outs? Meaning, billing for supplies, time it takes to process sendout/receive them back in, etc? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From fbozkurt <@t> gmail.com Wed Feb 19 12:38:17 2014 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Wed Feb 19 12:38:23 2014 Subject: [Histonet] aqueous mounting medium In-Reply-To: References: Message-ID: I'm using modified protocol. I'm storing it at 4C. *Aqueous Mounting Medium (glycerol gelatin)* *Kisser's**Kaiser's**Glycerol jelly**Modified*Gelatine10 g40 g65 g5 gDistilled water35 ml210 ml300 ml50 mlGlycerol (glycerin)30 ml250 ml100 ml50 mlPhenol (carbolic acid) 5 ml5 ml Mix gelatin and distilled water. Heat in 50 C oven until gelatin is dissolved (it takes about 30 minutes). Add glycerin and adjust pH to 7.0 using 1N NaOH (to avoid fading of hematoxylin). Store at room temperature. Warm to 37-40 C before mounting. Note: Modified protocol works well. from ihcworld.com On Wed, Feb 19, 2014 at 8:22 PM, Caroline Miller wrote: > I use prolong gold from life tech (formerly molecular probes and now part > of the world dominating thermofisher) without Dapi as I do that separately, > for IF and aquaslip from American master tech for non IF aqueous mounting > > Good luck! > Caroline > > Caroline Miller > Gladstone Institutes > www.gladstoneinstitutes.org > > Tel: 415 7342566 > Cell: 415 2187297 > > > > > > On Feb 19, 2014, at 10:11 AM, Hans B Snyder > wrote: > > > > Hello, > > > > I have one for ORO but not for IF. If someone has one for IF, I would > be interested also. > > > > Thank you > > > > Histologistics > > Hans B Snyder > > 508.308.7800 > > Hans@histologistics.com > > > >> On Feb 19, 2014, at 12:27, Jennifer MacDonald > wrote: > >> > >> Does anyone have a good "recipe" for aqueous mounting medium? > >> Thanks, > >> Jennifer > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 Asst. Prof. Dr. Mehmet Fatih BOZKURT Manager of Veterinary Diagnostic Laboratory Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16300-16301 vetanaliz@aku.edu.tr From McKenzie.Emily <@t> mhsil.com Wed Feb 19 12:46:17 2014 From: McKenzie.Emily <@t> mhsil.com (McKenzie, Emily) Date: Wed Feb 19 12:46:24 2014 Subject: [Histonet] Used Equipment Message-ID: Hello Histoneters!! Our facility is upgrading our IHC process. We are currently in the market for a used linear stainer (for hydrate and dehydrate) and/or a used coverslipper (glass). These items need to be fairly small since we don't have a lot of room to put equipment. If there are any facilities out there that have either of these and are willing to part with them please let me know. If you are not interested in parting with used equipment can someone suggest small linear strainers and small coverslipper's that aren't too expensive. Thank you for your help!!! Emily K. McKenzie BS, HT(ASCP) Memorial Medical Center?701 North First Street?Springfield, IL 62781 Ph: 217-788-3991?email: McKenzie.Emily@mhsil.com ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From ahc53 <@t> georgetown.edu Wed Feb 19 13:34:17 2014 From: ahc53 <@t> georgetown.edu (Anna Coffey) Date: Wed Feb 19 13:34:31 2014 Subject: [Histonet] Job Opportunity at Georgetown University Histopathology and Tissue Shared Resource Lab Message-ID: We have a new histology technician position that has just opened in the Histopathology and Tissue Shared Resource lab at Georgetown University's Lombardi Comprehensive Cancer Center. We're looking for someone with some lab experience (B.S. in biology, chemistry, or related field preferred) who is strongly motivated to gain more specialized histology experience, specifically with microtomy. This would be an ideal position for someone looking to fill their experience requirements for their HT/HTL certification. We are hoping to fill this position as quickly as possible. Please contact me with any questions. IMPORTANT: All interested applicants should submit applications directly through this website - http://www12.georgetown.edu/hr/employment_services/joblist/job_description.cfm?CategoryID=7&RequestNo=20140338 -- Anna Coffey Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu From sarahf3 <@t> hotmail.com Wed Feb 19 13:46:25 2014 From: sarahf3 <@t> hotmail.com (SARAH GIBSON) Date: Wed Feb 19 13:46:30 2014 Subject: [Histonet] Dried out liver bx's Message-ID: We are having problems with some of our small liver bx looking burned and dry. Does anyone have any suggestions on ways to fix this problem and any techniques while sectionly to get a better quality section. Thanks for any help. Sent from my iPad From KSimeone <@t> leavittmgt.com Wed Feb 19 13:47:34 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed Feb 19 13:47:38 2014 Subject: [Histonet] HIRING PT and per diem DELRAY BEACH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CAB94@vm-email.leavittmgt.com> Hi Histonetters! We are still interviewing for a part time tech here in our very busy east Delray FLORIDA Dermatology Lab (upwards of 80k cases/year). This is a permanent part time position with full time growth potential. PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES!!! Email your resume to lengimann@leavittmgt.com if interested. MUST BE LICENSED!!!! *part time position AND per diem available with FLEXIBLE TIMES (24 hour facility) *MUST be licensed as a FL histotechnologist or technician *MUST have at least 2 years experience (dermatology preferred) *very proficient in embedding and microtomy *experience in grossing and immunohistochemistry (BOND) a plus *must be self motivated and a team player (UNPROFESSIONALISM WILL NOT BE TOLERATED) *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm ksimeone@leavittmgt.com www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From fbozkurt <@t> gmail.com Wed Feb 19 13:49:18 2014 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Wed Feb 19 13:49:23 2014 Subject: [Histonet] Dried out liver bx's In-Reply-To: References: Message-ID: Hello, You can try less alcohol fixation and before cutting you can put blocks in wet-ice box. On Wed, Feb 19, 2014 at 9:46 PM, SARAH GIBSON wrote: > We are having problems with some of our small liver bx looking burned and > dry. Does anyone have any suggestions on ways to fix this problem and any > techniques while sectionly to get a better quality section. Thanks for any > help. > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 Asst. Prof. Dr. Mehmet Fatih BOZKURT Manager of Veterinary Diagnostic Laboratory Afyon Kocatepe University 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16300-16301 vetanaliz@aku.edu.tr From rperez <@t> medsurgpath.com Wed Feb 19 13:54:04 2014 From: rperez <@t> medsurgpath.com (Rolly Perez) Date: Wed Feb 19 13:55:47 2014 Subject: [Histonet] Cytolyt Solution Message-ID: Anyone knows how to make Cytolyt Solution? From COPPINM <@t> aruplab.com Wed Feb 19 13:56:53 2014 From: COPPINM <@t> aruplab.com (Coppin, Margaret) Date: Wed Feb 19 13:56:58 2014 Subject: [Histonet] Mesothelioma Control Message-ID: <448AA3F629599F4097B1E11A178B6C0696D4782C@EXMBX1.aruplab.net> Hello everyone, I am on a desperate search for a good mesothelioma control. Can anyone out there help? Either a block or even just a few unstained slides would be great! Thank you. Margaret G. Coppin, HT (ASCP) Technical Supervisor, Immunohistochemistry Integrated Oncology and Genetics Group II ARUP Laboratories 500 Chipeta Way Salt Lake City, UT 84108 ph. 801-583-2787 ext. 3869 email: coppinm@aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From wbenton <@t> cua.md Wed Feb 19 13:59:06 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Feb 19 13:59:29 2014 Subject: [Histonet] RE: Cytolyt Solution In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF445@CUAEXH1.GCU-MD.local> Hologic Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rolly Perez [rperez@medsurgpath.com] Sent: Wednesday, February 19, 2014 2:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytolyt Solution Anyone knows how to make Cytolyt Solution? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From TanyaAbbott <@t> catholichealth.net Wed Feb 19 14:12:07 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Feb 19 14:12:11 2014 Subject: [Histonet] Validating New IHC antibodies Message-ID: <852F7D2C14FB464D80E182B15DB138AF30637FF0@CHIEX006.CHI.catholichealth.net> What's everyone's go to # for Pos/Neg slides when validating new IHC antibodies? (Not counting Her2 and ER/PR, of course!) Thanks! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From immunoqueen <@t> yahoo.com Wed Feb 19 14:14:02 2014 From: immunoqueen <@t> yahoo.com (Jennifer Leigh) Date: Wed Feb 19 14:14:05 2014 Subject: [Histonet] affymetrix Anti-Mouse F4/80 antigen purified Message-ID: <1392840842.27449.YahooMailNeo@web120702.mail.ne1.yahoo.com> ?????????????All- ? ??????????????? Has anyone worked with the affymetrix Anti-Mouse F4/80 antigen (catalog #14-4801) in paraffin embedded tissue? The spec sheet indicates that proteinase treatment is needed. Any insight for working with this particular antibody would be very much appreciated. Thanks so much! ? Jennifer Oskins Jennifer L. Oskins "Until one has loved an animal, part of their soul remains unawakened....." From joelleweaver <@t> hotmail.com Wed Feb 19 16:26:40 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Feb 19 16:26:44 2014 Subject: [Histonet] Validating New IHC antibodies In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF30637FF0@CHIEX006.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF30637FF0@CHIEX006.CHI.catholichealth.net> Message-ID: minimum 10, usually 25 for binary scored-diagnostic markers. More in many cases if ASR. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TanyaAbbott@catholichealth.net > To: histonet@lists.utsouthwestern.edu > Date: Wed, 19 Feb 2014 20:12:07 +0000 > Subject: [Histonet] Validating New IHC antibodies > > What's everyone's go to # for Pos/Neg slides when validating new IHC antibodies? > (Not counting Her2 and ER/PR, of course!) > Thanks! > Tanya > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology > St. Joseph Medical Center > Reading, PA 19603-0316 > ph 610-378-2635 > fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Wed Feb 19 16:41:45 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed Feb 19 16:42:03 2014 Subject: [Histonet] Dried out liver bx's In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00B35B3@xmdb04.nch.kids> Hi Sarah, 90% of the time it is a collection issue. Do they leave it on filter (or any other absorbent) paper before placing in fixative. OR, heaven forbid, are they putting it on one of those alcohol wipes they use for disinfecting skin before biopsy? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SARAH GIBSON Sent: Thursday, 20 February 2014 6:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dried out liver bx's We are having problems with some of our small liver bx looking burned and dry. Does anyone have any suggestions on ways to fix this problem and any techniques while sectionly to get a better quality section. Thanks for any help. Sent from my iPad _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From schaundrawalton <@t> yahoo.com Wed Feb 19 17:06:16 2014 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Wed Feb 19 17:06:23 2014 Subject: [Histonet] Xylene Resistant Labels Message-ID: <5FF7D1D7-5F0D-40F1-8825-F32AA142F1D9@yahoo.com> Hey Histonetters! We are looking to make some cost effective efficiency changes in some of our outlying labs. We have one small lab currently hand writing slides and then printing paper labels and relabeling slides after staining. Does anyone know of xylene resistant labels that could be printed on a desktop printer or protective covers for paper labels? We'd like to eliminate the "double" labeling system for efficiency reasons. Any suggestions are welcome. Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor PathGroup Nashville, TN From liz <@t> premierlab.com Wed Feb 19 17:27:31 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Feb 19 17:29:16 2014 Subject: [Histonet] Xylene Resistant Labels In-Reply-To: <5FF7D1D7-5F0D-40F1-8825-F32AA142F1D9@yahoo.com> References: <5FF7D1D7-5F0D-40F1-8825-F32AA142F1D9@yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E02098@SBS2K8.premierlab.local> We use a printer that we purchased from General Data and their labels and they created a specialized software for us since we are a research lab. The labels work great in our hands. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton [schaundrawalton@yahoo.com] Sent: Wednesday, February 19, 2014 4:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Resistant Labels Hey Histonetters! We are looking to make some cost effective efficiency changes in some of our outlying labs. We have one small lab currently hand writing slides and then printing paper labels and relabeling slides after staining. Does anyone know of xylene resistant labels that could be printed on a desktop printer or protective covers for paper labels? We'd like to eliminate the "double" labeling system for efficiency reasons. Any suggestions are welcome. Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor PathGroup Nashville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbell <@t> fmh.org Wed Feb 19 17:36:52 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Wed Feb 19 17:36:57 2014 Subject: [Histonet] Dried out liver bx's In-Reply-To: References: Message-ID: Rough cut then soak in your waterbath for a minute or so then put back on ice to cool. Then section. U should get a beautiful ribbon :) Tasha Sent from my iPhone > On Feb 19, 2014, at 2:49 PM, "SARAH GIBSON" wrote: > > We are having problems with some of our small liver bx looking burned and dry. Does anyone have any suggestions on ways to fix this problem and any techniques while sectionly to get a better quality section. Thanks for any help. > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Wed Feb 19 20:35:16 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Feb 19 20:38:00 2014 Subject: [Histonet] aqueous mounting medium In-Reply-To: References: Message-ID: <5EFF028F99E64FF08063DDB9D66AF7DD@HP2010> to be used for what? - Lipids - ORO, Sudan black B? - Immunofluorescence - which dye? Different needs, different requirements. Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Jennifer MacDonald Sent: Wednesday, February 19, 2014 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] aqueous mounting medium Does anyone have a good "recipe" for aqueous mounting medium? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Feb 19 21:55:24 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Feb 19 21:55:29 2014 Subject: [Histonet] aqueous mounting medium In-Reply-To: <5EFF028F99E64FF08063DDB9D66AF7DD@HP2010> Message-ID: <4DA4DBC8-4C6D-4E06-A940-109510620AB3@mtsac.edu> Enzyme histochemistry > On Feb 19, 2014, at 6:42 PM, "Lee & Peggy Wenk" wrote: > > to be used for what? > - Lipids - ORO, Sudan black B? > - Immunofluorescence - which dye? > > Different needs, different requirements. > > Peggy A. Wenk, HTL(ASCP)SLS > > -----Original Message----- > From: Jennifer MacDonald > Sent: Wednesday, February 19, 2014 12:27 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] aqueous mounting medium > > Does anyone have a good "recipe" for aqueous mounting medium? > Thanks, > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Wed Feb 19 21:58:53 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Feb 19 21:58:57 2014 Subject: [Histonet] Dried out liver bx's In-Reply-To: Message-ID: <3CB386CE-B995-4224-B174-C48364AEA5AD@mtsac.edu> We had the same problem with GI biopsies. The problem was always with the same tech. She was blasting the blocks with freezing spray and they were "freezer burnt". > On Feb 19, 2014, at 11:49 AM, "SARAH GIBSON" wrote: > > We are having problems with some of our small liver bx looking burned and dry. Does anyone have any suggestions on ways to fix this problem and any techniques while sectionly to get a better quality section. Thanks for any help. > > Sent from my iPad > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From W.E.J.Hoekert <@t> olvg.nl Thu Feb 20 02:34:35 2014 From: W.E.J.Hoekert <@t> olvg.nl (Hoekert, W.E.J.) Date: Thu Feb 20 02:35:34 2014 Subject: [Histonet] Corpora amylacea are destroyed after antigen retrieval? References: <1392764394.97139.YahooMailNeo@web121204.mail.ne1.yahoo.com> Message-ID: <1190CB05C44B13409483514729C2FC3601F843C8@PAIT42.olvg.nl> I am not sure if you are talking about the Beta Amyloid antibody, but if you are: the best way to do the antigen retrieval is 3 minutes in formic acid. Willem ________________________________ Van: histonet-bounces@lists.utsouthwestern.edu namens Pirici Daniel Verzonden: di 18-2-2014 23:59 Aan: Histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Corpora amylacea are destroyed after antigen retrieval? Has anyone observed corpora amylacea on brain tissue being destroyed after antigen retrieval (for example microwaving in citrate buffer)? We are experiencing this problem, as we are interested to study these structures, and we are not sure if it is a common artifact when preparing slides for IHC... Thanks so much for your time! Daniel ________________________________________________________________________________________________ Pirici Nicolae Daniel, MD, PhD Department of Histology University of Medicine and Pharmacy Craiova Petru Rares Street 2, 200349 Craiova, Dolj Romania _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. From aikhanlab <@t> gmail.com Thu Feb 20 03:33:16 2014 From: aikhanlab <@t> gmail.com (Md. Aminul Islam Khan) Date: Thu Feb 20 03:33:26 2014 Subject: [Histonet] Choosing a tissue processor Message-ID: Hi everyone, Can you help us in choosing a tissue autoprocessor. At present we doing manual tissue processing and our work load is around 90 to 120 blocks per day which may increase gradually. These include tiny biopsies (e.g. gastric, pleural etc) to large specimens. Now we want to go for automation. People here are very poor for which we give the service at a low cost. They come from rural areas and staying for extradays in the city is very expensive for them. So we want to shorten the processing time as well. Also the technical hands are very scarce (i.e. if anything goes wrong with the autoprocessor). What would be the best technology for us? Microwave, linear, crousel or vacuum processor? Thanks in advance and regards, aikhan From histo <@t> skm.org.pk Thu Feb 20 04:45:26 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Thu Feb 20 04:46:24 2014 Subject: [Histonet] Choosing a tissue processor In-Reply-To: References: Message-ID: Dear Aminul Islam Khan, Shandon Citadel 2000 or Sakura is good for this work load. We are using Peloris Leica and Leica ASP 300, our workload are 900 to 1200 blocks /day. Regards, Muhammad Tahseen Senior Supervisor Histopathology SKMCH&RC Lahore Pakistan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Md. Aminul Islam Khan Sent: Thursday, February 20, 2014 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Choosing a tissue processor Hi everyone, Can you help us in choosing a tissue autoprocessor. At present we doing manual tissue processing and our work load is around 90 to 120 blocks per day which may increase gradually. These include tiny biopsies (e.g. gastric, pleural etc) to large specimens. Now we want to go for automation. People here are very poor for which we give the service at a low cost. They come from rural areas and staying for extradays in the city is very expensive for them. So we want to shorten the processing time as well. Also the technical hands are very scarce (i.e. if anything goes wrong with the autoprocessor). What would be the best technology for us? Microwave, linear, crousel or vacuum processor? Thanks in advance and regards, aikhan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Thu Feb 20 06:40:21 2014 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Thu Feb 20 06:41:00 2014 Subject: [Histonet] Xylene Resistant Labels Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760C477EE7@VHAV09MSGA3.v09.med.va.gov> We went with a slide printer. We have used the Sakura (ink) and Thermo (ribbon) printers. Both have their good/bad points. There was or may still be a CAP regulation that if you have a label on the slide that the information had to be visible on the slide underneath (would have to be looking from the back). The reason was that if the label fell off the slide would still have to be identifiable. Lisa White HT(ASCP) Supervisory HT James H. Quillen VAMC Corner of Veterans Way and Lamont VAMC Warehouse BLDG. 205 PO Box 4000 PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax From litepath2000 <@t> yahoo.com Thu Feb 20 07:47:40 2014 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Thu Feb 20 07:47:44 2014 Subject: [Histonet] 2014 Region 1 Symposium Message-ID: <1392904060.65011.YahooMailNeo@web140304.mail.bf1.yahoo.com> Region 1 Inc 2014 Annual? Meeting, April 5th & 6th @ The Renaissance Hotel at Patriot Place-Gillette Stadium Foxboro, Massachusetts Hosted by the Massachusetts Society of Histotechnology For a program and additional meeting info please contact the Massachusetts President Michael Dufault michaeldufault@aol.com Programs are also available on the following Region 1 States websites: Maine Society for Histotechnology http://www.mehisto.org/upcoming-seminars New York State Histotechnological Society http://www.nyhisto.org/annual-meetings/ ? ------- Luis Chiriboga Ph.D. President, New York State Histotechnological Society NYSHS Website: www.nyhisto.org NYSHS Message Board: http://tech.groups.yahoo.com/group/NYSHS1972/ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. From LBUSTAMANTE <@t> cvm.tamu.edu Thu Feb 20 08:31:02 2014 From: LBUSTAMANTE <@t> cvm.tamu.edu (Bustamante, Lin) Date: Thu Feb 20 08:31:05 2014 Subject: [Histonet] Texas Society for Histotechnology Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9011BADCFDC@CVMMB02.cvm.tamu.edu> Is there going to be a TSH meeting this year? PLEASE !!! I need to find out. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From relia1 <@t> earthlink.net Thu Feb 20 08:53:03 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Feb 20 08:53:07 2014 Subject: [Histonet] Histotechnology Professionals Day is March 10th! Message-ID: <00d201cf2e4b$752752d0$5f75f870$@earthlink.net> Hi Histonetters!! Did you know that March 10 is Histotechnology Professionals Day? I have a jpeg of the logo for Histotechnology Professionals Day. I would be happy to send it to you so you can share it with your fellow histotechs. They would probably love to have it. They will be able to print it out, Instagram it or post it to their Facebook page. Just let me know if you would like a copy. I would be happy to email it to you. I can also send you a link to the area on the NSH website where there are lots of fun ideas for Histotechnology Professionals Day. Have a great day! Thanks- Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/reliasolutions www.twitter.com/pamatrelia From vperez <@t> pathreflab.com Thu Feb 20 09:15:12 2014 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Thu Feb 20 09:15:21 2014 Subject: [Histonet] RE: Texas Society for Histotechnology In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC9011BADCFDC@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC9011BADCFDC@CVMMB02.cvm.tamu.edu> Message-ID: No TSH this year because NSH is being held in Austin. Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Thursday, February 20, 2014 8:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Texas Society for Histotechnology Is there going to be a TSH meeting this year? PLEASE !!! I need to find out. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From caithesketh <@t> gmail.com Thu Feb 20 11:00:14 2014 From: caithesketh <@t> gmail.com (Caitlin Hesketh) Date: Thu Feb 20 11:00:22 2014 Subject: [Histonet] Collagen 7 Message-ID: Does anyone know of a lab that performs an IHC of this antibody, and will do stains for other labs if we pay for it? Thanks! On Thursday, February 20, 2014, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: aqueous mounting medium (Hans B Snyder) > 2. Re: aqueous mounting medium (Caroline Miller) > 3. Billing (Abbott, Tanya) > 4. Re: aqueous mounting medium (Mehmet Fatih BOZKURT) > 5. Used Equipment (McKenzie, Emily) > 6. Job Opportunity at Georgetown University Histopathology and > Tissue Shared Resource Lab (Anna Coffey) > 7. Dried out liver bx's (SARAH GIBSON) > 8. HIRING PT and per diem DELRAY BEACH FL > (Delray Beach Pathology Kari Simeone) > 9. Re: Dried out liver bx's (Mehmet Fatih BOZKURT) > 10. Cytolyt Solution (Rolly Perez) > 11. Mesothelioma Control (Coppin, Margaret) > 12. RE: Cytolyt Solution (Walter Benton) > 13. Validating New IHC antibodies (Abbott, Tanya) > 14. affymetrix Anti-Mouse F4/80 antigen purified (Jennifer Leigh) > 15. RE: Validating New IHC antibodies (joelle weaver) > 16. RE: Dried out liver bx's (Tony Henwood (SCHN)) > 17. Xylene Resistant Labels (Schaundra Walton) > 18. RE: Xylene Resistant Labels (Elizabeth Chlipala) > 19. Re: Dried out liver bx's (Campbell, Tasha M.) > 20. Re: aqueous mounting medium (Lee & Peggy Wenk) > 21. Re: aqueous mounting medium (Jennifer MacDonald) > 22. Re: Dried out liver bx's (Jennifer MacDonald) > 23. RE: Corpora amylacea are destroyed after antigen retrieval? > (Hoekert, W.E.J.) > 24. Choosing a tissue processor (Md. Aminul Islam Khan) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 19 Feb 2014 13:11:41 -0500 > From: Hans B Snyder > Subject: Re: [Histonet] aqueous mounting medium > To: Jennifer MacDonald > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Hello, > > I have one for ORO but not for IF. If someone has one for IF, I would be > interested also. > > Thank you > > Histologistics > Hans B Snyder > 508.308.7800 > Hans@histologistics.com > > > On Feb 19, 2014, at 12:27, Jennifer MacDonald > wrote: > > > > Does anyone have a good "recipe" for aqueous mounting medium? > > Thanks, > > Jennifer > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Wed, 19 Feb 2014 10:22:58 -0800 > From: Caroline Miller > Subject: Re: [Histonet] aqueous mounting medium > To: Hans B Snyder > Cc: "histonet@lists.utsouthwestern.edu" > , Jennifer MacDonald > > Message-ID: > Content-Type: text/plain; charset=us-ascii > > I use prolong gold from life tech (formerly molecular probes and now part > of the world dominating thermofisher) without Dapi as I do that separately, > for IF and aquaslip from American master tech for non IF aqueous mounting > > Good luck! > Caroline > > Caroline Miller > Gladstone Institutes > www.gladstoneinstitutes.org > > Tel: 415 7342566 > Cell: 415 2187297 > > > > > > On Feb 19, 2014, at 10:11 AM, Hans B Snyder > wrote: > > > > Hello, > > > > I have one for ORO but not for IF. If someone has one for IF, I would > be interested also. > > > > Thank you > > > > Histologistics > > Hans B Snyder > > 508.308.7800 > > Hans@histologistics.com > > > >> On Feb 19, 2014, at 12:27, Jennifer MacDonald > wrote: > >> > >> Does anyone have a good "recipe" for aqueous mounting medium? > >> Thanks, > >> Jennifer > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Wed, 19 Feb 2014 18:24:50 +0000 > From: "Abbott, Tanya" > Subject: [Histonet] Billing > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 852F7D2C14FB464D80E182B15DB138AF30637E73@CHIEX006.CHI.catholichealth.net> > > Content-Type: text/plain; charset="us-ascii" > > Does anyone bill for a "technical component" for send outs? Meaning, > billing for supplies, time it takes to process sendout/receive them back > in, etc? > Thanks! > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology > St. Joseph Medical Center > Reading, PA 19603-0316 > ph 610-378-2635 > fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This electronic mail and any attached documents are intended solely for > the named addressee(s) and contain confidential information. If you are not > an addressee, or responsible for delivering this email to an addressee, you > have received this email in error and are notified that reading, copying, > or disclosing this email is prohibited. If you received this email in > error, immediately reply to the sender and delete the message completely > from your computer system. > > > ------------------------------ > > Message: 4 > Date: Wed, 19 Feb 2014 20:38:17 +0200 > From: Mehmet Fatih BOZKURT > Subject: Re: [Histonet] aqueous mounting medium > To: Caroline Miller , > Histonet@lists.utsouthwestern.edu > Message-ID: > NktSU+hmsrxXW+_0auTg@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > I'm using modified protocol. I'm storing it at 4C. > > > *Aqueous Mounting Medium (glycerol gelatin)* > > > *Kisser's**Kaiser's**Glycerol jelly**Modified*Gelatine10 g40 g65 g5 > gDistilled > water35 ml210 ml300 ml50 mlGlycerol (glycerin)30 ml250 ml100 ml50 mlPhenol > (carbolic acid) 5 ml5 ml > > > > > > Mix gelatin and distilled water. Heat in 50 C oven until gelatin is > dissolved (it takes about 30 minutes). Add glycerin and adjust pH to 7.0 > using 1N NaOH (to avoid fading of hematoxylin). Store at room temperature. > > > > Warm to 37-40 C before mounting. Note: Modified protocol works well. > > > > from ihcworld.com > > > > > > On Wed, Feb 19, 2014 at 8:22 PM, Caroline Miller < > cmiller@gladstone.ucsf.edu > > wrote: > > > I use prolong gold from life tech (formerly molecular probes and now part > > of the world dominating thermofisher) without Dapi as I do that > separately, > > for IF and aquaslip from American master tech for non IF aqueous mounting > > > > Good luck! > > Caroline > > > > Caroline Miller > > Gladstone Institutes > > www.gladstoneinstitutes.org > > > > Tel: 415 7342566 > > Cell: 415 2187297 > > > > > > > > > > > On Feb 19, 2014, at 10:11 AM, Hans B Snyder > > wrote: > > > > > > Hello, > > > > > > I have one for ORO but not for IF. If someone has one for IF, I would > > be interested also. > > > > > > Thank you > > > > > > Histologistics > > > Hans B Snyder > > > 508.308.7800 > > > Hans@histologistics.com > > > > > >> On Feb 19, 2014, at 12:27, Jennifer MacDonald > > wrote: > > >> > > >> Does anyone have a good "recipe" for aqueous mounting medium? > > >> Thanks, > > >> Jennifer > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet@lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Asst. Prof. Dr. Mehmet Fatih BOZKURT > Department of Pathology > Faculty of Veterinary Medicine > Afyon Kocatepe University > 03030, ANS Campus > Afyonkarahisar-TURKEY > Tel: +902722281312-16173/16237 > > Asst. Prof. Dr. Mehmet Fatih BOZKURT > Manager of Veterinary Diagnostic Laboratory > Afyon Kocatepe University > 03030, ANS Campus > Afyonkarahisar-TURKEY > Tel: +902722281312-16300-16301 > vetanaliz@aku.edu.tr > > > ------------------------------ > > Message: 5 > Date: Wed, 19 Feb 2014 12:46:17 -0600 > From: "McKenzie, Emily" > Subject: [Histonet] Used Equipment > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="koi8-r" > > Hello Histoneters!! > Our facility is upgrading our IHC process. We are currently in the market > for a used linear stainer (for hydrate and dehydrate) and/or a used > coverslipper (glass). These items need to be fairly small since we don't > have a lot of room to put equipment. If there are any facilities out there > that have either of these and are willing to part with them please let me > know. > If you are not interested in parting with used equipment can someone > suggest small linear strainers and small coverslipper's that aren't too > expensive. > Thank you for your help!!! > > Emily K. McKenzie BS, HT(ASCP) > > Memorial Medical Center 701 North First Street Springfield, IL 62781 > Ph: 217-788-3991 email: McKenzie.Emily@mhsil.com > > > > > ________________________________ > This message (including any attachments) contains confidential information > intended for a specific individual and purpose, and is protected by law. If > you are not the intended recipient, you should delete this message. Any > disclosure, copying, or distribution of this message, or the taking of any > action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 6 > Date: Wed, 19 Feb 2014 14:34:17 -0500 > From: Anna Coffey > Subject: [Histonet] Job Opportunity at Georgetown University > Histopathology and Tissue Shared Resource Lab > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > CALVW9z6-+AEYqNUQdkiEdA6TmNKY569wNh_C3ZvrdpVaasUxdg@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > We have a new histology technician position that has just opened in the > Histopathology and Tissue Shared Resource lab at Georgetown University's > Lombardi Comprehensive Cancer Center. We're looking for someone with some > lab experience (B.S. in biology, chemistry, or related field preferred) who > is strongly motivated to gain more specialized histology experience, > specifically with microtomy. This would be an ideal position for someone > looking to fill their experience requirements for their HT/HTL > certification. We are hoping to fill this position as quickly as possible. > Please contact me with any questions. > > IMPORTANT: All interested applicants should submit applications directly > through this website - > > http://www12.georgetown.edu/hr/employment_services/joblist/job_description.cfm?CategoryID=7&RequestNo=20140338 > > -- > Anna Coffey > Senior Histology Technician > Department of Oncology > Histopathology and Tissue Shared Resource > LR-10 Pre-Clinical Sciences Building > Lombardi Comprehensive Cancer Center > Georgetown University > 202-687-7890 > ahc53@georgetown.edu > > > ------------------------------ > > Message: 7 > Date: Wed, 19 Feb 2014 13:46:25 -0600 > From lpwenk <@t> sbcglobal.net Thu Feb 20 11:20:35 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Feb 20 11:20:36 2014 Subject: [Histonet] RE: Texas Society for Histotechnology In-Reply-To: References: <94B6DC15AAF2F046BF847D4C1CA9AAC9011BADCFDC@CVMMB02.cvm.tamu.edu> Message-ID: August 23 - 27, NSH Symposium in Austin, TX. NSH is still working on the program, but here's their website - so check back here often: http://www.histoconvention.org/index.cfm Peggy A. Wenk, HTL(ASCP)SLS -----Original Message----- From: Vanessa Perez Sent: Thursday, February 20, 2014 10:15 AM To: Bustamante, Lin ; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Texas Society for Histotechnology No TSH this year because NSH is being held in Austin. Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Thursday, February 20, 2014 8:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Texas Society for Histotechnology Is there going to be a TSH meeting this year? PLEASE !!! I need to find out. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Thu Feb 20 11:50:27 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Feb 20 11:50:33 2014 Subject: [Histonet] Subject: Histology Society of Ohio 2014 Symposium - Posting for a friend. Message-ID: <002f01cf2e64$3d81d580$b8858080$@earthlink.net> Subject: Histology Society of Ohio 2014 Symposium Please join us for the 2014 Histology Society of Ohio Symposium/Convention at the Hilton Garden Inn, Perrysburg, Ohio on April 4th and 5th. Attached is the 2014 brochure. There is a wonderful line-up of speakers and vendors. New this year with your registration by 3/5/2014 is free membership in HSO, Banquet and Box lunch with the vendors. Registration is easy on our website "Ohiohistology.org" using Paypal or you can still pay by check. Please pass this on to your co-workers, students, etc. Please don't hesitate to call, e-mail or text with any questions. Hope to see you in April. Mary Ann Day phone: 216-844-3834 Cell: 440-479-8466 For more info please visit their website at www.ohiohistology.org Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2014 is Histotechnology Professionals Day. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From BZIMMERM <@t> gru.edu Thu Feb 20 13:15:54 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Thu Feb 20 13:16:02 2014 Subject: [Histonet] HISTOPALOOZA April 25 - 27, 2014 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF812066@EX-MLB-03.ad.georgiahealth.edu> April will be here before you know it, so be sure and reserve your room at The Lodge and Spa at Callaway Gardens. Today's featured speaker is Skip Brown, NSH Historian. He's the guy you see at the NSH convention snapping all the pics. Skip is presenting two workshops, Optimization of Fixation and Processing in the 21st Century and Case Studies in Diagnostic Renal Histopathology. The first workshop, Case Studies in Diagnostic Renal Histopathology will be held on Friday, April 25th at 3:30pm. Participants will receive detailed information via handouts on the anatomy and physiology of the renal system. They will also learn the vital role that histology has with the pathologists as well as the clinicians. The second workshop will be on Saturday, April 26th at 1:30pm. This workshop will be great for people wanting to learn about fixation, including rapid fixation. This session is ideal for students involved in formal study in histology and a good review for those sitting for the Board exam. Please make sure you find Skip, introduce yourself, and have your picture made with him. You won't have to worry about selfie pics for Facebook, get Skip to do it!! I'm going to ask him if it's possible for him to Photoshop by thighs or double chin. LOL Looking forward to seeing all of you at Callaway Gardens. From smcbride <@t> andrew.cmu.edu Thu Feb 20 13:19:19 2014 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Thu Feb 20 13:19:24 2014 Subject: [Histonet] Xylene Resistant Labels In-Reply-To: <5FF7D1D7-5F0D-40F1-8825-F32AA142F1D9@yahoo.com> References: <5FF7D1D7-5F0D-40F1-8825-F32AA142F1D9@yahoo.com> Message-ID: <48C53CFB-D8E6-4B86-AB71-F92888A0696C@andrew.cmu.edu> Hi Schaundra, I have used the StainerBondz labels from Brady for quite a while, and am quite pleased with them. Good luck! Sean McBride Senior Scientific Specialist Bone Tissue Engineering Center Research Histology Services Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 571-989-BONE (m) 412-268-8275 (o) 412-268-8275 (fax) smcbride@andrew.cmu.edu On Feb 19, 2014, at 6:06 PM, Schaundra Walton wrote: > Hey Histonetters! > > We are looking to make some cost effective efficiency changes in some of our outlying labs. We have one small lab currently hand writing slides and then printing paper labels and relabeling slides after staining. Does anyone know of xylene resistant labels that could be printed on a desktop printer or protective covers for paper labels? We'd like to eliminate the "double" labeling system for efficiency reasons. Any suggestions are welcome. > > Thanks, > Schaundra Walton BS, HTL(ASCP) > Histology Supervisor > PathGroup > Nashville, TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Thu Feb 20 13:30:03 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Feb 20 13:30:25 2014 Subject: [Histonet] Arkansas Society for Histotechnology Spring Meeting - Next Week Message-ID: <370637424.96928.1392924603551.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> The meeting starts next Friday February 28th, and Saturday March 1st. The all day Histo Prep is on Saturday. We would like to invite anyone can join us to come on and see what we have to offer. We had Shane Jones doing Histology Registry Prep all day on Saturday for anyone planning to take the test this spring. Our program includes both beginning IHC and advanced techniques. ? Robert Skinner will be talking where Orthopedic Research and treatment are going in the future with interdisciplinary cooperation in many areas of medicine. Want to know the future of mobile Histology applications for our area. Dr Shree Sharma will discuss this with a glimpse at the coming applications. Safety for the new rules in handling waste and formaldehyde in Histology will be offered with Richard Best from Stericycle. A basic Microtomy workshop is being offered to help all of us cut better sections and improve our workplace for fewer repetitive injuries. Learn the Mysteries of Histology and how to solve them. These are the highlights of our meeting with other offerings also available. If you would like more information please contact me and we send you the full registration packet with the forms, program and abstracts to review. Thank You and Join Us! Pam Marcum From jpiche <@t> wtbyhosp.org Thu Feb 20 13:49:38 2014 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Thu Feb 20 13:49:42 2014 Subject: [Histonet] Retic stain kits Message-ID: <631955447A364B45B9458D2905635110A467F6B4@WIN08-MBX-01.wtbyhosp.org> Hello Everyone, Can anyone recommend a good retic staining kit that is reasonably priced and works well? Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From akelley <@t> path.wustl.edu Thu Feb 20 13:55:36 2014 From: akelley <@t> path.wustl.edu (Kelley, Amanda) Date: Thu Feb 20 13:55:45 2014 Subject: [Histonet] RE: HISTOPALOOZA April 25 - 27, 2014 In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF812066@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF812066@EX-MLB-03.ad.georgiahealth.edu> Message-ID: Billie what state is this? Amanda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie Sent: Thursday, February 20, 2014 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HISTOPALOOZA April 25 - 27, 2014 April will be here before you know it, so be sure and reserve your room at The Lodge and Spa at Callaway Gardens. Today's featured speaker is Skip Brown, NSH Historian. He's the guy you see at the NSH convention snapping all the pics. Skip is presenting two workshops, Optimization of Fixation and Processing in the 21st Century and Case Studies in Diagnostic Renal Histopathology. The first workshop, Case Studies in Diagnostic Renal Histopathology will be held on Friday, April 25th at 3:30pm. Participants will receive detailed information via handouts on the anatomy and physiology of the renal system. They will also learn the vital role that histology has with the pathologists as well as the clinicians. The second workshop will be on Saturday, April 26th at 1:30pm. This workshop will be great for people wanting to learn about fixation, including rapid fixation. This session is ideal for students involved in formal study in histology and a good review for those sitting for the Board exam. Please make sure you find Skip, introduce yourself, and have your picture made with him. You won't have to worry about selfie pics for Facebook, get Skip to do it!! I'm going to ask him if it's possible for him to Photoshop by thighs or double chin. LOL Looking forward to seeing all of you at Callaway Gardens. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From wsimons <@t> athensgastro.com Thu Feb 20 14:02:07 2014 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Thu Feb 20 14:02:11 2014 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUkU6IEhJU1RPUEFMT09aQSBBcHJpbCAyNSAtIDI3LCAyMDE0?= In-Reply-To: References: <7B3DEB32E69C034EACB479059C5DE3FF812066@EX-MLB-03.ad.georgiahealth.edu> Message-ID: <20140220200207.19553.qmail@server276.com> Georgia Society for Histotechnology www.histosearch.com/gsh Billie is so excited to share our great list of speakers, she forgot to add our link! I think she may be nervous about the photo op : ) Wanda K Simons, HT(ASCP) GSH President > -------Original Message------- > From: Kelley, Amanda > To: Zimmerman, Billie , histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: HISTOPALOOZA April 25 - 27, 2014 > Sent: Feb 20 '14 14:55 > > Billie what state is this? > Amanda > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zimmerman, Billie > Sent: Thursday, February 20, 2014 1:16 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HISTOPALOOZA April 25 - 27, 2014 > > April will be here before you know it, so be sure and reserve your room at The Lodge and Spa at Callaway Gardens. Today's featured speaker is Skip Brown, NSH Historian. He's the guy you see at the NSH convention snapping all the pics.??Skip is presenting two workshops, Optimization of Fixation and Processing in the 21st Century and Case Studies in Diagnostic Renal Histopathology. The first workshop, Case Studies in Diagnostic Renal Histopathology will be held on Friday, April 25th at 3:30pm.??Participants will receive detailed information via handouts on the anatomy and physiology of the renal system.??They will also learn the vital role that histology has with the pathologists??as well as the clinicians.??The second??workshop will be on Saturday, April 26th at 1:30pm. This workshop will be great for people wanting to learn about fixation, including rapid fixation. This session is ideal for students involved in formal study in histology and a good review for those sitting for the Board exam. > Please make sure you find Skip, introduce yourself, and have your picture made with him.??You won't have to worry about selfie pics for Facebook, get Skip to do it!! I'm going to ask him if it's possible for him to Photoshop by thighs or double chin. LOL Looking forward to seeing all of you at Callaway Gardens. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PAMarcum <@t> uams.edu Thu Feb 20 14:03:18 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Feb 20 14:03:28 2014 Subject: [Histonet] RE: Retic stain kits In-Reply-To: <631955447A364B45B9458D2905635110A467F6B4@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110A467F6B4@WIN08-MBX-01.wtbyhosp.org> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA320103B628A4@Mail2Node1.ad.uams.edu> I would try Polyscientific R&D. One thing we have an issue with on cost is the silver used in these kits is based on market value. If the price of silver is up so are the prices of silver based kits. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Thursday, February 20, 2014 1:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retic stain kits Hello Everyone, Can anyone recommend a good retic staining kit that is reasonably priced and works well? Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Richard.Cartun <@t> hhchealth.org Thu Feb 20 14:21:49 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Feb 20 14:22:01 2014 Subject: [Histonet] Chlamydia control tissue Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E0226D197@HHCEXCHMB03.hhcsystem.org> Does anyone have control tissue for Chlamydia that they can spare? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Bauer.Karen <@t> mayo.edu Thu Feb 20 14:56:59 2014 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Thu Feb 20 14:57:09 2014 Subject: [Histonet] Fungus Controls Message-ID: <6e55ab$8d6oon@ironport10.mayo.edu> Hi all, We are in need of some Fungus controls. Anyone have any extras to spare? Will do a trade... if we have anything available for you. Thank you very much, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org From tmcampbell <@t> fmh.org Thu Feb 20 15:03:25 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Thu Feb 20 15:03:31 2014 Subject: [Histonet] Retic stain kits In-Reply-To: <631955447A364B45B9458D2905635110A467F6B4@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110A467F6B4@WIN08-MBX-01.wtbyhosp.org> Message-ID: <89C590B8-94A4-4D43-BFDE-23D311C5AA6E@fmh.org> Poly scientific is the best. You can try Mercedes medical. They are probably cheaper. Sent from my iPhone > On Feb 20, 2014, at 2:52 PM, "Piche, Jessica" wrote: > > Hello Everyone, > > Can anyone recommend a good retic staining kit that is reasonably priced and works well? Thanks, > > Jessica Piche, HT(ASCP) > Waterbury Hospital > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vrivera <@t> westderm.com Thu Feb 20 15:38:05 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Thu Feb 20 15:38:09 2014 Subject: [Histonet] GI medicare reimbursement rates Message-ID: <3D4A471B82E7A44C87F6839732320D9F048558@VSPDMS-ITEXMB02.DMS.COM> Hello my tried and true histonetters: I could use some help finding average reimbursement rates for the codes that would be billed to GI specimens. Medicare reimbursement rates for 2014 for 88305, IHC Codes, and special stain codes TC/PC and Global. I was wonder if anyone out there has a cheat sheet they'd be willing to share? Once again, thank you in advance! Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) From pruegg <@t> ihctech.net Thu Feb 20 15:48:53 2014 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Thu Feb 20 15:48:55 2014 Subject: [Histonet] Poster at USCAP Message-ID: <00fa01cf2e85$8c754f20$a55fed60$@ihctech.net> Dear Colleagues, If you are attending USCAP and get a chance take a look at this poster. A couple of years ago I was involved in an IHC HIER study to compare work done near sea level to work done at an altitude above 5K feet, it is the first work of this kind since I wrote an article about HER2neu at altitude in 2001, as far as we know. This study will be presented as a poster at USCAP in San Diego, March 3rd # 2108. This study shows IHC on the same 10 antibodies and same tissue, comparing HIER at different temperatures both at low altitude (work done at Emory U in Atlanta, Georgia with Dr. Cohen) and at an altitude above 5K feet (work done at IHCtech in Aurora, Colorado with Patsy Ruegg). Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com From mucram11 <@t> comcast.net Thu Feb 20 15:52:27 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Feb 20 15:52:36 2014 Subject: [Histonet] Poster at USCAP In-Reply-To: <00fa01cf2e85$8c754f20$a55fed60$@ihctech.net> Message-ID: <1781026515.99595.1392933147230.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Cool, I am sorry I will get to go and see it.? I don't think we look at those issues enough.? I would even include work done in arid and humid areas as possible issues.? Pam Marcum ----- Original Message ----- From: pruegg@ihctech.net To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 20, 2014 3:48:53 PM Subject: [Histonet] Poster at USCAP Dear Colleagues, ? If you are attending USCAP and get a chance take a look at this poster. ? A couple of years ago I was involved in an IHC HIER study to compare work done near sea level to work done at an altitude above 5K feet, it is the first work of this kind since I wrote an article about HER2neu at altitude in 2001, as far as we know. ? This study will be presented as a poster at USCAP in San Diego, March 3rd # 2108. ?This study shows IHC on the same 10 antibodies and same tissue, comparing HIER at different temperatures both at low altitude (work done at Emory U in Atlanta, Georgia with Dr. Cohen) and at an altitude above 5K feet (work done at IHCtech in Aurora, Colorado with Patsy Ruegg). ? Cheers, Patsy ? ? Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 ? pruegghm@hotmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbell <@t> fmh.org Thu Feb 20 16:26:00 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Thu Feb 20 16:26:08 2014 Subject: [Histonet] GI medicare reimbursement rates In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F048558@VSPDMS-ITEXMB02.DMS.COM> References: <3D4A471B82E7A44C87F6839732320D9F048558@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <3EACD82A-AF74-478A-A9B9-BB332E164AFB@fmh.org> Medicare reimbursement is around $30 per biopsy. Sent from my iPhone > On Feb 20, 2014, at 4:42 PM, "Vincent Rivera" wrote: > > Hello my tried and true histonetters: > > I could use some help finding average reimbursement rates for the codes that would be billed to GI specimens. Medicare reimbursement rates for 2014 for 88305, IHC Codes, and special stain codes TC/PC and Global. I was wonder if anyone out there has a cheat sheet they'd be willing to share? > > Once again, thank you in advance! > > Vincent Rivera, HT (ASCP), QIHC, QLS > Histopathology Supervisor > West Dermatology Pathology Laboratory > vrivera@westderm.com > 714-924-7240 (Lab) > 714-390-0906 (Cell) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From blayjorge <@t> gmail.com Thu Feb 20 17:16:14 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Thu Feb 20 17:16:19 2014 Subject: [Histonet] Could someone recommend (either from experience or word of mouth) helpful chemistry listservers? Message-ID: Hello Histoneters: Could someone recommend (either from experience or word of mouth) helpful chemistry listservers? Please, feel free to reply directly to my email ( blayjorge@gmail.com). Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From amber.mckenzie <@t> gastrodocs.net Thu Feb 20 22:43:09 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Feb 20 22:43:14 2014 Subject: [Histonet] Grossing initials/IHC and Special Stain control check-off In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F048558@VSPDMS-ITEXMB02.DMS.COM> References: <3D4A471B82E7A44C87F6839732320D9F048558@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0112468A6B@JERRY.Gia.com> 2 questions: 1. How do you document who grosses in your specimens? Are their initials typed on the report under gross description or are they hand written on the paperwork that comes with the bx's - path requisition? 2. How do you document that the special stain/IHC (+) and (-) slides worked/failed? Do the pathologists sign off on each one or a tech? Do you have a certain paper that you send with your slides that they sign off on and give back to the supervisor to keep? Thanks! From emleslie <@t> ufl.edu Fri Feb 21 09:33:08 2014 From: emleslie <@t> ufl.edu (MERTON,EMILY MACKENZI) Date: Fri Feb 21 09:34:12 2014 Subject: [Histonet] Xylene-free labs? Message-ID: Hello, How common are xylene-free labs in the histo world? I worked for 3 years at a research service histology lab, and am now in a research lab. I miss histology, and have considered getting certified so that I can have a career in it, but my sensitivity to xylene has made me hesitate. Is there any movement towards more labs being xylene-free, or might it be difficult to find employment? Thank you, Emily Merton emleslie@ufl.edu Research Assistant University of Florida Genetics Institute Howard Hughes Medical Institute From relia1 <@t> earthlink.net Fri Feb 21 10:23:06 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Feb 21 10:23:28 2014 Subject: [Histonet] RELIA Hot JOB ALERT!!! Histotechnician/Histotechnologist needed - Private Lab Atlanta, GA Message-ID: <000401cf2f21$3403e350$9c0ba9f0$@earthlink.net> Hi Histonetters!!! I hope you are all gearing up for a fun weekend!! I have a new opportunity that I am excited to tell you about! RELIA Solutions has been engaged to recruit a top notch histology tech for a growing private lab in Atlanta, GA. ASCP HT/HTL or elig and 2-5 years of histology experience is required. IHC and grossing experience is a PLUS. My client offers competitive salary, excellent benefits and a great team to work with. If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 If we place someone you refer you will earn a referral fee! RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1@earthlink.net and include subscribe in the subject line. Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2014 is Histotechnology Professionals Day. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 <@t> earthlink.net Fri Feb 21 10:44:24 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Feb 21 10:44:32 2014 Subject: [Histonet] TGIF Things are really cooking around here today. ANOTHER RELIA HOT Histology Job Alert!! Histotechs needed in Boston!! Message-ID: <001101cf2f24$2e723b00$8b56b100$@earthlink.net> Hi Histonetters!!! I hope you are all gearing up for a fun weekend!! I have a new opportunity that I am excited to tell you about! RELIA Solutions has been engaged to recruit a top notch histology tech for a growing private lab in Boston, MA. ASCP HT/HTL or elig and 2 years of histology experience is required, grossing experience is a PLUS. My client offers competitive salary, excellent benefits and a great team to work with. Opportunity for advancement and a brand new lab!!! If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542 If we place someone you refer you will earn a referral fee! RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1@earthlink.net and include subscribe in the subject line. Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2014 is Histotechnology Professionals Day. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Dorothy.L.Webb <@t> HealthPartners.Com Fri Feb 21 12:18:26 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Feb 21 12:19:42 2014 Subject: [Histonet] sign offs Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302BF6DAE5D18@HPEMX3.HealthPartners.int> We print a staining report from our IHC and/or special stain equipment and the tech initials he/she checked control and the pathologist initials it OK and returns the form of which we keep for 2 years. If it is a manual stain, we have a form we made to fill out and do the same with. For us, it was an area we were sited for several years ago at inspection and now cover it well. Dorothy Webb ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Michael.LaFriniere <@t> ccplab.com Fri Feb 21 12:42:54 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Fri Feb 21 12:43:01 2014 Subject: [Histonet] Histotech position In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302BF6DAE5D18@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4302BF6DAE5D18@HPEMX3.HealthPartners.int> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29653DD1@AHCMSASEXCH03.my.ahc.local> In search of Histotech, certified or not....NO HEAD HUNTERS PLEASE! Tuesday-Sat, midnights....good pay/benefits and working conditions in a newly built state of the art histology lab; must be "team player orientated" demonstrate integrity and love histology. This is not just a job but a career position for the engaged tech. Our main lab is part of the Adventist Healthcare family located in Silver Spring Maryland. Our clients are several hospitals, physician offices and research based. Contact me if this type of position may be of interest Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com From Bauer.Karen <@t> mayo.edu Sat Feb 22 12:42:04 2014 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen L.) Date: Sat Feb 22 12:42:16 2014 Subject: [Histonet] RE: Fungus Controls In-Reply-To: <6e55ab$8d6oon@ironport10.mayo.edu> References: <6e55ab$8d6oon@ironport10.mayo.edu> Message-ID: <6e55ab$8dglvf@ironport10.mayo.edu> Thanks to everyone who replied to my fungus control request. I had a lot of great responses!! :) Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Thursday, February 20, 2014 2:57 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Fungus Controls Importance: Low Hi all, We are in need of some Fungus controls. Anyone have any extras to spare? Will do a trade... if we have anything available for you. Thank you very much, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Mon Feb 24 08:04:40 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Mon Feb 24 08:07:30 2014 Subject: [Histonet] RE: Fungus Controls In-Reply-To: <6e55ab$8dglvf@ironport10.mayo.edu> References: <6e55ab$8d6oon@ironport10.mayo.edu> <6e55ab$8dglvf@ironport10.mayo.edu> Message-ID: <12ECD7346266D74691EC2BFC75285E452F3FB67A@BFL323E10.pathmdlabs.local> I am in need of fungus controls, if there are still some out there..... I can provide shipping costs. Thanks! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Saturday, February 22, 2014 10:42 AM To: Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Fungus Controls Importance: Low Thanks to everyone who replied to my fungus control request. I had a lot of great responses!! :) Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Thursday, February 20, 2014 2:57 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Fungus Controls Importance: Low Hi all, We are in need of some Fungus controls. Anyone have any extras to spare? Will do a trade... if we have anything available for you. Thank you very much, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From immunoqueen <@t> yahoo.com Mon Feb 24 09:17:56 2014 From: immunoqueen <@t> yahoo.com (Jennifer Leigh) Date: Mon Feb 24 09:17:59 2014 Subject: [Histonet] Proteinase K EIER Solutions Message-ID: <1393255076.3309.YahooMailNeo@web120705.mail.ne1.yahoo.com> ????? All- ? ??????????????I am interested in?Proteinase K EIER applications and was curiouis if?ready-made solutions are preferred or preparation from powder and if so, how do you prepare and store the solutions? I have used the DakyCytomation ready-made Proteinase K in the past (years ago) but I am not sure if this is the most cost-effective way. Thank you so much for any ideas! ? Jennifer Oskins Jennifer L. Oskins "Until one has loved an animal, part of their soul remains unawakened....." From fbozkurt <@t> gmail.com Mon Feb 24 09:21:23 2014 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Mon Feb 24 09:21:33 2014 Subject: [Histonet] Proteinase K EIER Solutions In-Reply-To: <1393255076.3309.YahooMailNeo@web120705.mail.ne1.yahoo.com> References: <1393255076.3309.YahooMailNeo@web120705.mail.ne1.yahoo.com> Message-ID: There is good protocol on ihcworld website. I had prepared five years ago. I'm still using it. 24 ?ub 2014 17:18 tarihinde "Jennifer Leigh" yazd?: > > All- > > I am interested in Proteinase K EIER applications and was curiouis if ready-made solutions are preferred or preparation from powder and if so, how do you prepare and store the solutions? I have used the DakyCytomation ready-made Proteinase K in the past (years ago) but I am not sure if this is the most cost-effective way. Thank you so much for any ideas! > > Jennifer Oskins > > Jennifer L. Oskins > "Until one has loved an animal, part of their soul remains unawakened....." > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From imhyper13 <@t> aol.com Mon Feb 24 09:34:12 2014 From: imhyper13 <@t> aol.com (imhyper13@aol.com) Date: Mon Feb 24 09:34:15 2014 Subject: [Histonet] University of Michigan... Message-ID: <8D0FF8D00FE7F67-1FA8-19982@webmail-vd007.sysops.aol.com> I am hoping to connect with a histo tech from the University of Michigan concerning the new LIS they just implemented there. If anyone knows of a contact there, I sure would appreciate it. Thank you From tbraud <@t> holyredeemer.com Mon Feb 24 09:37:50 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Feb 24 09:37:54 2014 Subject: [Histonet] Gross Tech and IHC Documentation In-Reply-To: <20140221172233.8C2E51E8040@trendmess-svr.holyredeemer.local> References: <20140221172233.8C2E51E8040@trendmess-svr.holyredeemer.local> Message-ID: 2 answers to 2 questions The initials of the grossing tech or pathologist appears directly under the gross dictation. If we have an IHC fail, the tech documents it on the printed run worksheet. We keep all printed worksheets. The pathologist has a daily review sheet that is reviewed and signed monthly by me as the supv. We don't turn any non-working stains to the pathologist. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 16 Date: Fri, 21 Feb 2014 04:43:09 +0000 From: Amber McKenzie Subject: [Histonet] Grossing initials/IHC and Special Stain control check-off 2 questions: 1. How do you document who grosses in your specimens? Are their initials typed on the report under gross description or are they hand written on the paperwork that comes with the bx's - path requisition? 2. How do you document that the special stain/IHC (+) and (-) slides worked/failed? Do the pathologists sign off on each one or a tech? Do you have a certain paper that you send with your slides that they sign off on and give back to the supervisor to keep? Thanks! ***************************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From mmorales <@t> adrian.edu Mon Feb 24 09:45:13 2014 From: mmorales <@t> adrian.edu (Marti Morales) Date: Mon Feb 24 09:45:40 2014 Subject: [Histonet] University of Michigan... In-Reply-To: <8D0FF8D00FE7F67-1FA8-19982@webmail-vd007.sysops.aol.com> References: <8D0FF8D00FE7F67-1FA8-19982@webmail-vd007.sysops.aol.com> Message-ID: You probably want to talk to Chris Edwards. fishon@umich.edu Here is their link and info. http://medicine.umich.edu/medschool/research/office-research/biomedical-research-core-facilities/microscopy-image-analysis/contact-us-locations Bests, Marti On Mon, Feb 24, 2014 at 10:34 AM, wrote: > > I am hoping to connect with a histo tech from the University of Michigan > concerning the new LIS they just implemented there. If anyone knows of a > contact there, I sure would appreciate it. > Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Marti Morales-Ensign, PhD Asst. Professor of Biology Adrian College 110 S. Madison St Adrian, MI 49221 517-265-5161 ext. 4040 mmorales@adrian.edu From Sruby <@t> 4path.com Mon Feb 24 12:16:48 2014 From: Sruby <@t> 4path.com (Stephen G. Ruby) Date: Mon Feb 24 12:16:55 2014 Subject: [Histonet] RE: Histonet Digest, Vol 123, Issue 26 In-Reply-To: References: Message-ID: We can provide some excellent fungal control blocks. Trade? We are in need of some: Prostate carcinoma / HGPIN (from prostate resection, not needles) ERG positive prostate carcinoma H. pylori gastric Drop an e-mail to : SRuby@4path.com Stephen G. Ruby, MD, MBA, FCAP President and Medical Director, 4path, Ltd. Value, Service and Commitment...Beyond the Diagnosis V: 1-877-884-7284 F: 708-929-4330 www.4path.com 9050 W. 81st Street, Justice, IL 60458 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, February 24, 2014 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 123, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Fungus Controls (Laurie Colbert) 2. Proteinase K EIER Solutions (Jennifer Leigh) 3. Re: Proteinase K EIER Solutions (Mehmet Fatih BOZKURT) 4. University of Michigan... (imhyper13@aol.com) 5. Gross Tech and IHC Documentation (Terri Braud) 6. Re: University of Michigan... (Marti Morales) ---------------------------------------------------------------------- Message: 1 Date: Mon, 24 Feb 2014 14:04:40 +0000 From: Laurie Colbert Subject: [Histonet] RE: Fungus Controls To: "Bauer, Karen L." , "'histonet@lists.utsouthwestern.edu'" Message-ID: <12ECD7346266D74691EC2BFC75285E452F3FB67A@BFL323E10.pathmdlabs.local> Content-Type: text/plain; charset="us-ascii" I am in need of fungus controls, if there are still some out there..... I can provide shipping costs. Thanks! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Saturday, February 22, 2014 10:42 AM To: Bauer, Karen L.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Fungus Controls Importance: Low Thanks to everyone who replied to my fungus control request. I had a lot of great responses!! :) Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Thursday, February 20, 2014 2:57 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Fungus Controls Importance: Low Hi all, We are in need of some Fungus controls. Anyone have any extras to spare? Will do a trade... if we have anything available for you. Thank you very much, Karen Karen Bauer, MHA, HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.karen@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 24 Feb 2014 07:17:56 -0800 (PST) From: Jennifer Leigh Subject: [Histonet] Proteinase K EIER Solutions To: Histonet Netserver Message-ID: <1393255076.3309.YahooMailNeo@web120705.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 ????? All- ? ??????????????I am interested in?Proteinase K EIER applications and was curiouis if?ready-made solutions are preferred or preparation from powder and if so, how do you prepare and store the solutions? I have used the DakyCytomation ready-made Proteinase K in the past (years ago) but I am not sure if this is the most cost-effective way. Thank you so much for any ideas! ? Jennifer Oskins Jennifer L. Oskins "Until one has loved an animal, part of their soul remains unawakened....." ------------------------------ Message: 3 Date: Mon, 24 Feb 2014 17:21:23 +0200 From: Mehmet Fatih BOZKURT Subject: Re: [Histonet] Proteinase K EIER Solutions To: Jennifer Leigh , Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 There is good protocol on ihcworld website. I had prepared five years ago. I'm still using it. 24 ??ub 2014 17:18 tarihinde "Jennifer Leigh" yazd??: > > All- > > I am interested in Proteinase K EIER applications and > was curiouis if ready-made solutions are preferred or preparation from powder and if so, how do you prepare and store the solutions? I have used the DakyCytomation ready-made Proteinase K in the past (years ago) but I am not sure if this is the most cost-effective way. Thank you so much for any ideas! > > Jennifer Oskins > > Jennifer L. Oskins > "Until one has loved an animal, part of their soul remains unawakened....." > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 24 Feb 2014 10:34:12 -0500 (EST) From: imhyper13@aol.com Subject: [Histonet] University of Michigan... To: histonet@lists.utsouthwestern.edu Message-ID: <8D0FF8D00FE7F67-1FA8-19982@webmail-vd007.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I am hoping to connect with a histo tech from the University of Michigan concerning the new LIS they just implemented there. If anyone knows of a contact there, I sure would appreciate it. Thank you ------------------------------ Message: 5 Date: Mon, 24 Feb 2014 10:37:50 -0500 From: "Terri Braud" Subject: [Histonet] Gross Tech and IHC Documentation To: Message-ID: Content-Type: text/plain; charset="us-ascii" 2 answers to 2 questions The initials of the grossing tech or pathologist appears directly under the gross dictation. If we have an IHC fail, the tech documents it on the printed run worksheet. We keep all printed worksheets. The pathologist has a daily review sheet that is reviewed and signed monthly by me as the supv. We don't turn any non-working stains to the pathologist. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 16 Date: Fri, 21 Feb 2014 04:43:09 +0000 From: Amber McKenzie Subject: [Histonet] Grossing initials/IHC and Special Stain control check-off 2 questions: 1. How do you document who grosses in your specimens? Are their initials typed on the report under gross description or are they hand written on the paperwork that comes with the bx's - path requisition? 2. How do you document that the special stain/IHC (+) and (-) slides worked/failed? Do the pathologists sign off on each one or a tech? Do you have a certain paper that you send with your slides that they sign off on and give back to the supervisor to keep? Thanks! ***************************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ------------------------------ Message: 6 Date: Mon, 24 Feb 2014 10:45:13 -0500 From: Marti Morales Subject: Re: [Histonet] University of Michigan... To: imhyper13@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 You probably want to talk to Chris Edwards. fishon@umich.edu Here is their link and info. http://medicine.umich.edu/medschool/research/office-research/biomedical-research-core-facilities/microscopy-image-analysis/contact-us-locations Bests, Marti On Mon, Feb 24, 2014 at 10:34 AM, wrote: > > I am hoping to connect with a histo tech from the University of > Michigan concerning the new LIS they just implemented there. If > anyone knows of a contact there, I sure would appreciate it. > Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Marti Morales-Ensign, PhD Asst. Professor of Biology Adrian College 110 S. Madison St Adrian, MI 49221 517-265-5161 ext. 4040 mmorales@adrian.edu ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 123, Issue 26 ***************************************** From TanyaAbbott <@t> catholichealth.net Mon Feb 24 13:06:52 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Feb 24 13:07:01 2014 Subject: [Histonet] pH sticks Message-ID: <852F7D2C14FB464D80E182B15DB138AF306473A7@CHIEX005.CHI.catholichealth.net> Is anyone aware if there are pH indicator strips that read to the 0.1? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From ryeo <@t> foxmail.wchosp.org Tue Feb 25 06:41:43 2014 From: ryeo <@t> foxmail.wchosp.org (Richard Yeo) Date: Tue Feb 25 06:41:51 2014 Subject: [Histonet] (no subject) Message-ID: <4512AE914CB84C198F8A477B7E6E25A9.MAI@foxmail.wchosp.org> Hey Histonet, Can any one out there on histonet tell me the salary r grossing histologist? And also the regions that these s Thanks Richard Yeo ryeo@foxmail.wchos Histology Manager Wooster Community Hospital 1761 Bea Wooster Ohio 44691 330 263 8563 [1]ryeo@foxmail.wshosp.org References 1. 3D"mailto:ryeo From Kelly.Pairan <@t> nationwidechildrens.org Tue Feb 25 08:29:20 2014 From: Kelly.Pairan <@t> nationwidechildrens.org (Pairan, Kelly) Date: Tue Feb 25 08:29:27 2014 Subject: [Histonet] AFB Control Blocks Message-ID: <10EFB578346B744AB255AA4972B9195069175E13@L1PERDWXMB03.childrensroot.net> Good Morning, We are currently cutting controls from our last few AFB control blocks. We are wondering if anyone has any they can spare? Please contact me at kelly.pairan@nationwidechildrens.org. We are prepared to pay for the shipping. Thanks! Kelly From Bruce_Palmatier <@t> vwr.com Tue Feb 25 09:01:53 2014 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Tue Feb 25 09:02:21 2014 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 02/26/2014) Message-ID: I am out of the office until 02/26/2014. I will be out of the office from February 24th until February 26th. I will be checking emails periodically and will attempt to reply to your message within 24 hours. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 123, Issue 25" sent on 2/25/2014 6:45:39 AM. This is the only notification you will receive while this person is away. From jamie <@t> prometheushealthcare.com Tue Feb 25 10:34:08 2014 From: jamie <@t> prometheushealthcare.com (Jamie Buxton) Date: Tue Feb 25 10:36:25 2014 Subject: [Histonet] Histology Manager Opening in TN Message-ID: <022201cf3247$69770bb0$3c652310$@prometheushealthcare.com> Currently recruiting for a Histology Manager with a top lab in Tennessee. For immediate consideration and additional information, please feel free to contact me at Jamie@prometheushealthcare.com From wbenton <@t> cua.md Tue Feb 25 11:54:46 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Feb 25 11:54:54 2014 Subject: [Histonet] Searching for Available Histo Position in New Mexico Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF483@CUAEXH1.GCU-MD.local> Does anyone know of any positions in the Albuquerque, New Mexico? I have a colleague looking for a position there. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From BZIMMERM <@t> gru.edu Tue Feb 25 13:53:05 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Feb 25 13:53:14 2014 Subject: [Histonet] HISTOPALOOZA April 25- 27, 2014 Georgia Society for Histology Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8139FC@EX-MLB-03.ad.georgiahealth.edu> Hello everyone! I hope everyone has had a chance to visit our website to register for the Histopalooza. If not,please reserve your room today. The contact person is Taylor Reames in the reservation department and her number is 706-489-3359. The featured speaker today is William DeSalvo. Bill has given many informative workshops and his topic at Callaway is titled "Histologic Process Improvement- Tissue Processing from Conventional Overnight to Continuous Rapid. Tissue processing protocols will be discussed along with open discussion about tissue processing instrumentation. Bill will include a discussion of LEAN and Six-Sigma tools. This presentation will allow you to gain a new perspective on tissue processing and create precision in your lab. I've learned a tidbit about Bill along the way. He is a cigar aficionado. I suppose you could say he's "The Other Bill". LOL Wanda, don't strangle me!! Billie Zimmerman GSH secretary http://www.histosearch.com/gsh/symposium.html From amber.mckenzie <@t> gastrodocs.net Tue Feb 25 16:45:13 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Feb 25 16:45:17 2014 Subject: [Histonet] Block/Slide retention for peds cases In-Reply-To: References: <20140221172233.8C2E51E8040@trendmess-svr.holyredeemer.local> Message-ID: <5A33C952BB67F4468AF1F36D739212BC011246A1FC@JERRY.Gia.com> Are there any regulations specifying any difference of holding blocks/slides for peds cases? Are they treated the same as adults cases and held 10-12 years or are they needed to be kept longer? In the block/slide storage room, are there any regulations on temp or humidity? Thanks! From numberfife <@t> gmail.com Tue Feb 25 17:27:24 2014 From: numberfife <@t> gmail.com (Stephanie Moore) Date: Tue Feb 25 17:27:30 2014 Subject: [Histonet] Email list Message-ID: Good Afternoon, I would like to know how I can be taken off the email list for Histonet. Thank you, *Stephanie Huff, B.S., HTL(ASCP)CM* From one_angel_secret <@t> yahoo.com Tue Feb 25 17:36:23 2014 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Tue Feb 25 17:36:35 2014 Subject: [Histonet] Block/Slide retention for peds cases In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011246A1FC@JERRY.Gia.com> References: <20140221172233.8C2E51E8040@trendmess-svr.holyredeemer.local> <5A33C952BB67F4468AF1F36D739212BC011246A1FC@JERRY.Gia.com> Message-ID: <0241AE30-D843-4CC2-ACAA-77726119A14D@yahoo.com> Regulation wise age makes no difference for how long you keep them. It's the same. And yes as I recall the regulation states " climate controlled room" I don't recall any specific temperature being mentioned but I've always interpreted that as your blocks shouldnt melt or freeze etc . I'm on the move now or I would hunt that regulation up for you. Hope this helps Kim D Sent from my iPhone On Feb 25, 2014, at 5:45 PM, Amber McKenzie wrote: > > Are there any regulations specifying any difference of holding blocks/slides for peds cases? Are they treated the same as adults cases and held 10-12 years or are they needed to be kept longer? > > In the block/slide storage room, are there any regulations on temp or humidity? > > Thanks! > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cindy.deriso <@t> yale.edu Wed Feb 26 07:00:39 2014 From: cindy.deriso <@t> yale.edu (DeRiso, Cynthia) Date: Wed Feb 26 07:00:45 2014 Subject: [Histonet] BRCA1 testing by IHC Message-ID: Asking this question from our molecular lab: We're trying to find a lab that does BRCA1 testing by IHC (not sequencing) Can you post this on HistoNet and see if anyone knows who does this? The reagent exists, so someone must be using it. Thanks In advance Cindy DeRiso Yale University From kjgada <@t> gmail.com Wed Feb 26 07:14:38 2014 From: kjgada <@t> gmail.com (Komal Gada) Date: Wed Feb 26 07:14:42 2014 Subject: [Histonet] HT Academic Position in Orlando, FL Message-ID: Good Morning! Keiser University is seeking a Histotechnology Clinical Coordinator at their Orlando campus. For more info visit http://keiseruniversityevergladesuniversity.hodesiq.com/job_detail.asp?JobID=4257983&user_id= Thanks, Komal Komal Gada, BS, HT/HTL(ASCP), QIHC Histotechnology University Department Chair Histotechnology Program Director - Orlando Keiser University 5600 Lake Underhill Rd. Orlando, FL 32807 From kgrobert <@t> rci.rutgers.edu Wed Feb 26 09:57:33 2014 From: kgrobert <@t> rci.rutgers.edu (kgrobert@rci.rutgers.edu) Date: Wed Feb 26 09:57:39 2014 Subject: [Histonet] Equipment recommendations, please Message-ID: Which microtomes and embedding centers are you using out there? I am looking at the Leica RM2265 (since we're a research lab) and the Thermo Scientific Finesse (We have one and I like it!), but I am also open to suggestions. Thank you so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From TanyaAbbott <@t> catholichealth.net Wed Feb 26 10:36:42 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Feb 26 10:36:57 2014 Subject: [Histonet] Gross only Message-ID: <852F7D2C14FB464D80E182B15DB138AF306482C5@CHIEX005.CHI.catholichealth.net> How does everyone bill for "Gross Only"? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From BSullivan <@t> virtua.org Wed Feb 26 10:45:13 2014 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Wed Feb 26 10:45:18 2014 Subject: [Histonet] RE: Gross only In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF306482C5@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF306482C5@CHIEX005.CHI.catholichealth.net> Message-ID: <6932520047F7EE46B512E9801344F16080001740@ExchangeMB-1.Virtua.org> Using the code 88300 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Wednesday, February 26, 2014 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross only How does everyone bill for "Gross Only"? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From joelleweaver <@t> hotmail.com Wed Feb 26 10:45:26 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Feb 26 10:45:30 2014 Subject: [Histonet] Gross only In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF306482C5@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF306482C5@CHIEX005.CHI.catholichealth.net> Message-ID: do you mean the code? 2014= 88300 Joelle Weaver MAOM, HTL (ASCP) QIHC > From: TanyaAbbott@catholichealth.net > To: histonet@lists.utsouthwestern.edu > Date: Wed, 26 Feb 2014 16:36:42 +0000 > Subject: [Histonet] Gross only > > How does everyone bill for "Gross Only"? > Thanks! > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology > St. Joseph Medical Center > Reading, PA 19603-0316 > ph 610-378-2635 > fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Wed Feb 26 10:48:33 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Feb 26 10:48:58 2014 Subject: [Histonet] RE: Gross only In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF306482C5@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF306482C5@CHIEX005.CHI.catholichealth.net> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF4A1@CUAEXH1.GCU-MD.local> It should be 88300 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya [TanyaAbbott@catholichealth.net] Sent: Wednesday, February 26, 2014 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross only How does everyone bill for "Gross Only"? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From fbozkurt <@t> gmail.com Wed Feb 26 10:55:30 2014 From: fbozkurt <@t> gmail.com (Mehmet Fatih BOZKURT) Date: Wed Feb 26 10:55:35 2014 Subject: [Histonet] Equipment recommendations, please In-Reply-To: References: Message-ID: Leica for Microtomy*, thermo histostar for embedding 26 ?ub 2014 18:53 tarihinde "Mehmet Fatih BOZKURT" yazd?: > Leica for Microsoft, histostar for embedding. > 26 ?ub 2014 17:58 tarihinde yazd?: > >> >> Which microtomes and embedding centers are you using out there? I am >> looking at the Leica RM2265 (since we're a research lab) and the Thermo >> Scientific Finesse (We have one and I like it!), but I am also open to >> suggestions. >> >> Thank you so much! >> Kathleen >> >> Principal Lab Technician >> Neurotoxicology Labs >> Molecular Pathology Facility Core >> Dept of Pharmacology & Toxicology >> Rutgers, the State University of NJ >> 41 B Gordon Road >> Piscataway, NJ 08854 >> (848) 445-1443 >> FAX (732) 445-6905 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From shive003 <@t> umn.edu Wed Feb 26 11:42:50 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Feb 26 11:42:55 2014 Subject: [Histonet] Email list In-Reply-To: References: Message-ID: >From the Histonet guidelines when one subscribes: If you ever want to unsubscribe or change your options (eg, switch to or from digest mode, change your password, etc.), visit your subscription page at: http://lists.utsouthwestern.edu/mailman/options/histonet/shive003%40umn.edu -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu On Tue, Feb 25, 2014 at 5:27 PM, Stephanie Moore wrote: > Good Afternoon, > > I would like to know how I can be taken off the email list for Histonet. > > Thank you, > > > *Stephanie Huff, B.S., HTL(ASCP)CM* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Vickroy.Jim <@t> mhsil.com Wed Feb 26 12:06:35 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Feb 26 12:06:42 2014 Subject: [Histonet] Leasing an Electron Microscope Message-ID: Anybody know of any Transmission Electron Microscope Companies that lease Transmission Electron Microscopes? If so please contact me. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From tbraud <@t> holyredeemer.com Wed Feb 26 12:26:03 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Feb 26 12:26:07 2014 Subject: [Histonet] Slide Storage In-Reply-To: <20140226172140.2F1241E8099@trendmess-svr.holyredeemer.local> References: <20140226172140.2F1241E8099@trendmess-svr.holyredeemer.local> Message-ID: CAP regs Anatomic Pathology ANP.23700 Storage Organization Under the notes section it states "The storage area for blocks should be cool to prevent blocks from melting together. Cytology CYP.017100 Slide Storage Under the notes section it states, "Cytopathology slides should be stored at room temperature for optimal preservation. Room temperature is often approximated at 20?C or 70?F. In scientific contexts, it usually denotes a range between 20 and 23.5 ?C (68.0 and 74.3 ?F) with an average of 21 ?C (70 ?F). Some instrumentation, such as your IHC instrument may have a specific Room Temp designation. Obviously, this is another measurement that must be recorded for CAP. And as far as I know, there are no national regulations requiring different retention times for peds records, but states could vary. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. Block/Slide retention for peds cases (Amber McKenzie) On Feb 25, 2014, at 5:45 PM, Amber McKenzie wrote: >Are there any regulations specifying any difference of holding blocks/slides for peds cases? Are they treated the same as adults cases and held 10-12 years or are they needed to be kept longer? In the block/slide storage room, are there any regulations on temp or humidity? Thanks! ***** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Timothy.Morken <@t> ucsfmedctr.org Wed Feb 26 12:26:41 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Feb 26 12:26:53 2014 Subject: [Histonet] RE: Leasing an Electron Microscope In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF3675AA0A@ex07.net.ucsf.edu> James, even if a particular instrument company does not, there are leasing companies that specialize in setting up lease agreements for just about anything. For example, a quick search found this one. I haven't used any, but some labs use such companies when they don't have the capital budget for something. http://www.telelease.com/asset-finance/scientific-equipment-leasing Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, February 26, 2014 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leasing an Electron Microscope Anybody know of any Transmission Electron Microscope Companies that lease Transmission Electron Microscopes? If so please contact me. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Wed Feb 26 12:41:31 2014 From: JMitchell <@t> uwhealth.org (Mitchell Jean A) Date: Wed Feb 26 12:41:35 2014 Subject: [Histonet] RE: Leasing an Electron Microscope In-Reply-To: <761E2B5697F795489C8710BCC72141FF3675AA0A@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF3675AA0A@ex07.net.ucsf.edu> Message-ID: <16F90B93CA23D446980B3D591FD02DAD0DD10B@UWHC-MBX14.uwhis.hosp.wisc.edu> James: Depending upon the amount of EM work your have - another option is to "lease" scoping time from another facility in your area that maintains an EM scope. My lab pays for scoping time on a per hour basis. You of course have to work with the owner of the scope for availability but it works out well for us (and we don't have to maintain anything). Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, February 26, 2014 12:27 PM To: 'Vickroy, Jim' Cc: Histonet Subject: [Histonet] RE: Leasing an Electron Microscope James, even if a particular instrument company does not, there are leasing companies that specialize in setting up lease agreements for just about anything. For example, a quick search found this one. I haven't used any, but some labs use such companies when they don't have the capital budget for something. http://www.telelease.com/asset-finance/scientific-equipment-leasing Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, February 26, 2014 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leasing an Electron Microscope Anybody know of any Transmission Electron Microscope Companies that lease Transmission Electron Microscopes? If so please contact me. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CObregon <@t> mhs.net Wed Feb 26 13:01:53 2014 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Wed Feb 26 13:02:34 2014 Subject: [Histonet] Poly Scientific Paraffin Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F27BE4558@MHSEXMB05.mhs.net> Afternoon Histoneters, I was just wondering if anyone is having issues with the paraffin from Poly Scientific? Our last shipment, the pellets look a lot smaller and it's not melting at the 60 degrees we originally had it programed! Vendor is recommending we increase the tempuerature 2 degrees. Is anyone else having issues with this paraffin? Thanks! Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From tgenade <@t> gmail.com Wed Feb 26 13:02:50 2014 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Wed Feb 26 13:02:53 2014 Subject: [Histonet] manual tissue processing protocol Message-ID: Hello, I need some input on a manual tissue processing protocol I have received. (5 columns: reagents; tiny to large tissues...) Reagents\embryoes\small tissue\medium tissue\large tissue 70% EtOH\30 min--1hr\1 hr--ON\2hr--ON\4hr--ON 95% EtOH\30 min--1hr\1 hr\1hr\2hr 95% EtOH\30 min\30 min\1 hr\1 hr 100% EtOH\30 min\1 hr\30 min\1 hr 100% EtOH\10 min\30 min\30 min\1 hr toluene\30 min\1 hr\1hr\1 hr toluene\none\30 min\30 min\1 hr till clear parafin infiltration\1hr all parafin infiltration\1 hr\1 hr\2hr\2hr EM400\1 hr\1hr\1hr\2+ hr I would be replacing the toluene with methylbenzoate. Are there any anticipated issues? Is this a good protocol so far as producing good quality tissue for sectioning and analysis? I am not sure what is meant by "small" and "large" tissue. My fish are about 5 cm in length and about 1 cm thick. Would they be classed as small? Is it necessary using two different types of paraffin : Surgipath infiltrating paraffin Cat # 01400 (4-5 lb/bag) and Surgipath embedding paraffin? For the manual infiltration: would a beaker of paraffin on a 60oC hotplate and stirrer (on low) be OK? I would be fixing with PFA and decalcidying with 30% EDTA. Might refixing in the PFA after decal be a good idea to make sure the tissue integrity is maintained? I would probably stored fixed, decalsified samples in 70% EtOH until I can process fully. Any one think there would be complications from doing this? Thanks From tony.auge <@t> gmail.com Wed Feb 26 13:15:06 2014 From: tony.auge <@t> gmail.com (Tony Auge) Date: Wed Feb 26 13:15:12 2014 Subject: [Histonet] Equipment recommendations, please In-Reply-To: References: Message-ID: The Thermo Shandon Finesse is my favorite microtome. Wish I had one at my lab... I've never really had a preference for embedding consoles. As long as it's ambidextrous it's fine with me, I'm a lefty. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com From m_chadwell <@t> hotmail.com Wed Feb 26 15:56:36 2014 From: m_chadwell <@t> hotmail.com (margaret chadwell) Date: Wed Feb 26 16:09:05 2014 Subject: [Histonet] Input on Sakura E150 Purchase Message-ID: Sent from Windows Mail Hello Histonet, Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it?s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can?t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla, Ca From hans <@t> histologistics.com Wed Feb 26 16:24:12 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Wed Feb 26 16:24:21 2014 Subject: [Histonet] Input on Sakura E150 Purchase In-Reply-To: References: Message-ID: I used one about 5 years ago with minimal issues. The only problem was finding parts to fix it. On one occasion we had to have someone fabricate a part to repair the unit. Other than that it worked well and was easy to maintain. Hope that helps Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Wed, Feb 26, 2014 at 4:56 PM, margaret chadwell wrote: > > > > > > > > Sent from Windows Mail > > Hello Histonet, > > Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it's a reliable unit, and parts are available for servicing. > > Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can't buy new. Thank you in advance! > > Margaret Chadwell > > LIAI > > La Jolla, Ca > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Wed Feb 26 16:24:20 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Feb 26 16:24:25 2014 Subject: [Histonet] Input on Sakura E150 Purchase In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E054FE@SBS2K8.premierlab.local> I have a VIP 300 from 1998 and it's still going strong. We have had a few repairs over the years the most recent having to replace the pump, its 16 years old and still functioning fine. As far as I am concerned it?s a reliable unit, we use it frequently. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of margaret chadwell Sent: Wednesday, February 26, 2014 2:57 PM To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] Input on Sakura E150 Purchase Sent from Windows Mail Hello Histonet, Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it?s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can?t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla, Ca From mpence <@t> grhs.net Thu Feb 27 11:14:05 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 27 11:14:26 2014 Subject: [Histonet] Disposable blade holder Message-ID: Need your help! I am looking for a disposable blade holder for a Microm HM355S microtome. A new one is over $2000 and I just need the front part of the blade holder (which they don't sell separately). I would buy a used (but in good condition) so I would have some extra parts. If anyone has on of these microtomes setting around would you consider selling the blade holder? Thanks, Mike Pence From Lisa.Freeman <@t> fda.hhs.gov Thu Feb 27 11:46:39 2014 From: Lisa.Freeman <@t> fda.hhs.gov (Freeman, Lisa*) Date: Thu Feb 27 11:46:48 2014 Subject: [Histonet] microscope information Message-ID: <0F928833A4CE744793039B781368BED0133DAE77@FDSWP3313.fda.gov> I am currently looking for a microscope to use at the sectioning stations. We have to locate tissues such as parathyroid glands and mammary glands at sectioning, as well as general QC. Any opinions on which is the best for this purpose? Thank you, Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson, AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov From rjbuesa <@t> yahoo.com Thu Feb 27 11:56:48 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 27 11:56:52 2014 Subject: [Histonet] microscope information In-Reply-To: <0F928833A4CE744793039B781368BED0133DAE77@FDSWP3313.fda.gov> Message-ID: <1393523808.4971.YahooMailBasic@web120401.mail.ne1.yahoo.com> ANY microscope with decent objectives will do. Even used microscopes you can buy in eBay. The problem is not with the microscope but how the HTs will be able to locate those structures while still embedded in the paraffin. Ren? J. -------------------------------------------- On Thu, 2/27/14, Freeman, Lisa* wrote: Subject: [Histonet] microscope information To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, February 27, 2014, 12:46 PM I am currently looking for a microscope to use at the sectioning stations. We have to locate tissues such as parathyroid glands and mammary glands at sectioning, as well as general QC. Any opinions on which is the best for this purpose? Thank you, Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson, AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Feb 27 11:57:45 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Feb 27 11:57:50 2014 Subject: [Histonet] RE: microscope information In-Reply-To: <0F928833A4CE744793039B781368BED0133DAE77@FDSWP3313.fda.gov> References: <0F928833A4CE744793039B781368BED0133DAE77@FDSWP3313.fda.gov> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05504@SBS2K8.premierlab.local> Lisa We purchased some Leica Student scopes for this purpose one for each of the sectioning stations. They are Leica DM750's. They are really nice, we do have a couple other microscopes we purchased previous to the DM750's but they were not as nice as these. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Freeman, Lisa* Sent: Thursday, February 27, 2014 10:47 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microscope information I am currently looking for a microscope to use at the sectioning stations. We have to locate tissues such as parathyroid glands and mammary glands at sectioning, as well as general QC. Any opinions on which is the best for this purpose? Thank you, Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson, AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail2 <@t> gmail.com Thu Feb 27 12:15:51 2014 From: renafail2 <@t> gmail.com (Rena Fail) Date: Thu Feb 27 12:15:55 2014 Subject: [Histonet] manual tissue processing protocol In-Reply-To: References: Message-ID: Hi, One mm thick is tiny, 2 mm small 1 cm is rather thick. Since you are manually processing run one sample to test your protocol or 2 if you want to see the difference between the shorter method and the longer. *protocol. .Complete dehydration is dependent on the volume, type of tissue and more importantly, the thickness of the tissue. You can check the tissue before infiltrating with paraffin.to see if it has cleared, If it appears transparent (like a stained glass window.) proceed with paraffin infiltration. * *Rena Fail* On Wed, Feb 26, 2014 at 2:02 PM, Tyrone Genade wrote: > Hello, > > I need some input on a manual tissue processing protocol I have received. > > (5 columns: reagents; tiny to large tissues...) > > Reagents\embryoes\small tissue\medium tissue\large tissue > 70% EtOH\30 min--1hr\1 hr--ON\2hr--ON\4hr--ON > 95% EtOH\30 min--1hr\1 hr\1hr\2hr > 95% EtOH\30 min\30 min\1 hr\1 hr > 100% EtOH\30 min\1 hr\30 min\1 hr > 100% EtOH\10 min\30 min\30 min\1 hr > toluene\30 min\1 hr\1hr\1 hr > toluene\none\30 min\30 min\1 hr till clear > parafin infiltration\1hr all > parafin infiltration\1 hr\1 hr\2hr\2hr > EM400\1 hr\1hr\1hr\2+ hr > > I would be replacing the toluene with methylbenzoate. Are there any > anticipated issues? Is this a good protocol so far as producing good > quality tissue for sectioning and analysis? > > I am not sure what is meant by "small" and "large" tissue. My fish are > about 5 cm in length and about 1 cm thick. Would they be classed as small? > > Is it necessary using two different types of paraffin : Surgipath > infiltrating paraffin Cat # 01400 (4-5 lb/bag) and Surgipath embedding > paraffin? For the manual infiltration: would a beaker of paraffin on a > 60oC hotplate and stirrer (on low) be OK? > > I would be fixing with PFA and decalcidying with 30% EDTA. Might refixing > in the PFA after decal be a good idea to make sure the tissue integrity is > maintained? I would probably stored fixed, decalsified samples in 70% EtOH > until I can process fully. Any one think there would be complications from > doing this? > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From robin3139 <@t> aol.com Thu Feb 27 12:22:35 2014 From: robin3139 <@t> aol.com (Robin) Date: Thu Feb 27 12:22:41 2014 Subject: [Histonet] RE: microscope information In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E05504@SBS2K8.premierlab.local> References: <0F928833A4CE744793039B781368BED0133DAE77@FDSWP3313.fda.gov> <14E2C6176416974295479C64A11CB9AE019C79E05504@SBS2K8.premierlab.local> Message-ID: <6E81E747-7648-4F47-8E1D-ECCD83CA7C6E@aol.com> Sent from my iPhoneohiuB On Feb 27, 2014, at 12:57 PM, Elizabeth Chlipala wrote: > Lisa From meg_g <@t> poczta.onet.pl Thu Feb 27 12:21:46 2014 From: meg_g <@t> poczta.onet.pl (Magda G) Date: Thu Feb 27 12:25:35 2014 Subject: [Histonet] RE: Histonet Digest, Vol 123, Issue 29 . Input on Sakura E150 Purchase (margaret chadwell) Message-ID: <001f01cf33e8$c8388410$58a98c30$@poczta.onet.pl> Hello, We used to have one of E150, but during our last PM our technician told me that they no longer make any parts for it so if it will break it may be impossible to be fixed. Maggie From JWatson <@t> gnf.org Thu Feb 27 12:54:29 2014 From: JWatson <@t> gnf.org (James Watson) Date: Thu Feb 27 12:54:34 2014 Subject: [Histonet] microscope information In-Reply-To: <1393523808.4971.YahooMailBasic@web120401.mail.ne1.yahoo.com> References: <0F928833A4CE744793039B781368BED0133DAE77@FDSWP3313.fda.gov> <1393523808.4971.YahooMailBasic@web120401.mail.ne1.yahoo.com> Message-ID: If you drop the condenser down so Koehler illumination is not aligned, this give you a pseudo phase contrast. You can see many structures in tissue due to the different density of the cells. So I would recommend a microscope that has a condenser that can be moved up and down. We have 2 old Olympus BH2 in our Microtomy area. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 27, 2014 9:57 AM To: Histonet@lists.utsouthwestern.edu; Lisa*Freeman Subject: Re: [Histonet] microscope information ANY microscope with decent objectives will do. Even used microscopes you can buy in eBay. The problem is not with the microscope but how the HTs will be able to locate those structures while still embedded in the paraffin. Ren? J. -------------------------------------------- On Thu, 2/27/14, Freeman, Lisa* wrote: Subject: [Histonet] microscope information To: "Histonet@lists.utsouthwestern.edu" Date: Thursday, February 27, 2014, 12:46 PM I am currently looking for a microscope to use at the sectioning stations. We have to locate tissues such as parathyroid glands and mammary glands at sectioning, as well as general QC. Any opinions on which is the best for this purpose? Thank you, Lisa Freeman, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson, AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Thu Feb 27 13:34:25 2014 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Feb 27 13:34:33 2014 Subject: [Histonet] New GHS labels Message-ID: I'm getting ready for my CAP inspection and I came along information about the new Global warning labels that were to replace the NFPA labels we currently use for our chemicals. Is everyone using these or can we still use the NFPA label and placards? I remember hearing about it at a seminar just shortly after out inspection in 2012 and never thought of it again. Thanks, Cheri Cheryl Miller HT ASCP Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From vperez <@t> pathreflab.com Thu Feb 27 13:53:59 2014 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Thu Feb 27 13:54:06 2014 Subject: [Histonet] RE: New GHS labels In-Reply-To: References: Message-ID: The global hazard chemical labels are for shipping purposes only on the bottles from the companies. The reagents made for in the lab that are put in containers can still use the same hazard labels you use now with the diamond. Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, February 27, 2014 1:34 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] New GHS labels I'm getting ready for my CAP inspection and I came along information about the new Global warning labels that were to replace the NFPA labels we currently use for our chemicals. Is everyone using these or can we still use the NFPA label and placards? I remember hearing about it at a seminar just shortly after out inspection in 2012 and never thought of it again. Thanks, Cheri Cheryl Miller HT ASCP Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Feb 27 13:55:53 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Feb 27 13:55:58 2014 Subject: [Histonet] RE: New GHS labels In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391681A2D0EB@IBMB7Exchange.digestivespecialists.com> Here is what I have in my notes for the new standards. I think you're probably safe with what you currently have as long as you've don't the training that should have been done by Dec 1st 2013. The first compliance date of the revised HCS is December 1, 2013. By that time employers must have trained their workers on the new label elements and the SDS format. All hazardous chemicals shipped after June 1, 2015, must be labeled with specified elements including pictograms, signal words and hazard and precautionary statements. However, manufacturers, importers, and distributors may start using the new labeling system in the revised HCS before the June 1, 2015 effective date if they so choose. Until the June 1, 2015 effective date, manufacturers, importers and distributors may maintain compliance with the requirements of HazCom 1994 or the revised standard. Distributors may continue to ship containers labeled by manufacturers or importers (but not by the distributor themselves) in compliance with the HazCom 1994 until December 1, 2015. Linda Linda Blazek HT (ASCP) GI Pathology of Dayton Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, February 27, 2014 2:34 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] New GHS labels I'm getting ready for my CAP inspection and I came along information about the new Global warning labels that were to replace the NFPA labels we currently use for our chemicals. Is everyone using these or can we still use the NFPA label and placards? I remember hearing about it at a seminar just shortly after out inspection in 2012 and never thought of it again. Thanks, Cheri Cheryl Miller HT ASCP Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Feb 27 13:59:12 2014 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Thu Feb 27 13:59:18 2014 Subject: [Histonet] RE: New GHS labels In-Reply-To: References: Message-ID: Per OSHA: As of June 1, 2015, all labels will be required to have pictograms, a signal word, hazard and precautionary statements, the product identifier, and supplier identification. A sample revised HCS label, identifying the required label elements, is shown on the right. Supplemental information can also be provided on the label as needed. This applies to manufacturers shipping products. OSHA's website under hazard communication lists a table with effective dates required under the Hazardous Communication Standard. Hope it helps! Stacy McLaughlin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, February 27, 2014 2:34 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] New GHS labels I'm getting ready for my CAP inspection and I came along information about the new Global warning labels that were to replace the NFPA labels we currently use for our chemicals. Is everyone using these or can we still use the NFPA label and placards? I remember hearing about it at a seminar just shortly after out inspection in 2012 and never thought of it again. Thanks, Cheri Cheryl Miller HT ASCP Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Feb 27 14:20:30 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 27 14:20:36 2014 Subject: [Histonet] Disposable Blade Holder Message-ID: Need your help! I am looking for a disposable blade holder for a Microm HM355S microtome. A new one is over $2000 and I just need the front part of the blade holder (which they don't sell separately). I would buy a used (but in good condition) so I would have some extra parts. If anyone has on of these microtomes setting around would you consider selling the blade holder? Thanks, Mike Pence From cmiller <@t> physlab.com Thu Feb 27 14:22:00 2014 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Feb 27 14:24:17 2014 Subject: [Histonet] RE: New GHS labels In-Reply-To: References: , Message-ID: Thank you all for your help. Our Safety Officer quit and I don't see anything except her notes from the seminar. I will pass this on to the new safety person. Thanks, Cheri Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________________ From: Stacy McLaughlin [Stacy_McLaughlin@cooley-dickinson.org] Sent: Thursday, February 27, 2014 1:59 PM To: Cheri Miller; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: New GHS labels Per OSHA: As of June 1, 2015, all labels will be required to have pictograms, a signal word, hazard and precautionary statements, the product identifier, and supplier identification. A sample revised HCS label, identifying the required label elements, is shown on the right. Supplemental information can also be provided on the label as needed. This applies to manufacturers shipping products. OSHA's website under hazard communication lists a table with effective dates required under the Hazardous Communication Standard. Hope it helps! Stacy McLaughlin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, February 27, 2014 2:34 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] New GHS labels I'm getting ready for my CAP inspection and I came along information about the new Global warning labels that were to replace the NFPA labels we currently use for our chemicals. Is everyone using these or can we still use the NFPA label and placards? I remember hearing about it at a seminar just shortly after out inspection in 2012 and never thought of it again. Thanks, Cheri Cheryl Miller HT ASCP Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From robin3139 <@t> aol.com Thu Feb 27 14:50:09 2014 From: robin3139 <@t> aol.com (Robin) Date: Thu Feb 27 14:50:17 2014 Subject: [Histonet] Z Message-ID: <75B63F84-EA49-45DB-90C7-FBC8982539E1@aol.com> Sent from my iPhone From shive003 <@t> umn.edu Thu Feb 27 15:35:29 2014 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Feb 27 15:35:33 2014 Subject: [Histonet] Email list In-Reply-To: References: Message-ID: Stephanie, et al.... Oops, the previous email in this string was an answer on how to unsubscribe from the Histonet. I copied and pasted the link from my own information email, not realizing that it contained MY Histonet email address on the end. Please DISREGARD the link (or delete the link ending after '/histonet/' where my email address is listed and THEN use it to unsubscribe). Sorry - I think the polar vortex has partially frozen my brain this winter! Jan Shivers UMN Vet Diag Lab St. Paul, MN On Wed, Feb 26, 2014 at 11:42 AM, Jan Shivers wrote: > From the Histonet guidelines when one subscribes: > > If you ever want to unsubscribe or change your options (eg, switch to > or from digest mode, change your password, etc.), visit your > subscription page at: > > > http://lists.utsouthwestern.edu/mailman/options/histonet/shive003%40umn.edu > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003@umn.edu > > > > On Tue, Feb 25, 2014 at 5:27 PM, Stephanie Moore wrote: > >> Good Afternoon, >> >> I would like to know how I can be taken off the email list for Histonet. >> >> Thank you, >> >> >> *Stephanie Huff, B.S., HTL(ASCP)CM* >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > > From one_angel_secret <@t> yahoo.com Thu Feb 27 17:50:48 2014 From: one_angel_secret <@t> yahoo.com (Kim Donadio) Date: Thu Feb 27 17:51:01 2014 Subject: [Histonet] manual tissue processing protocol In-Reply-To: References: Message-ID: <674368B0-A8A2-426B-84C5-8B0D10229C65@yahoo.com> I agree with Renee here about testing one before. However those are thick and I'm supposing your fish have scales? This also might slow the process down. My best guess is you're going to need a much longer processing time. And since you don't have vacuum I personally think that thickness is going to need a few hours. I've never processed a fish though take that for what it's worth. Good luck Sent from my iPhone On Feb 27, 2014, at 1:15 PM, Rena Fail wrote: > Hi, > > One mm thick is tiny, 2 mm small 1 cm is rather thick. Since you are > manually processing run one sample to test your protocol or 2 if you want > to see the difference between the shorter method and the longer. *protocol. > .Complete dehydration is dependent on the volume, type of tissue and more > importantly, the thickness of the tissue. You can check the tissue before > infiltrating with paraffin.to see if it has > cleared, If it appears transparent (like a stained glass window.) proceed > with paraffin infiltration. * > *Rena Fail* > > > On Wed, Feb 26, 2014 at 2:02 PM, Tyrone Genade wrote: > >> Hello, >> >> I need some input on a manual tissue processing protocol I have received. >> >> (5 columns: reagents; tiny to large tissues...) >> >> Reagents\embryoes\small tissue\medium tissue\large tissue >> 70% EtOH\30 min--1hr\1 hr--ON\2hr--ON\4hr--ON >> 95% EtOH\30 min--1hr\1 hr\1hr\2hr >> 95% EtOH\30 min\30 min\1 hr\1 hr >> 100% EtOH\30 min\1 hr\30 min\1 hr >> 100% EtOH\10 min\30 min\30 min\1 hr >> toluene\30 min\1 hr\1hr\1 hr >> toluene\none\30 min\30 min\1 hr till clear >> parafin infiltration\1hr all >> parafin infiltration\1 hr\1 hr\2hr\2hr >> EM400\1 hr\1hr\1hr\2+ hr >> >> I would be replacing the toluene with methylbenzoate. Are there any >> anticipated issues? Is this a good protocol so far as producing good >> quality tissue for sectioning and analysis? >> >> I am not sure what is meant by "small" and "large" tissue. My fish are >> about 5 cm in length and about 1 cm thick. Would they be classed as small? >> >> Is it necessary using two different types of paraffin : Surgipath >> infiltrating paraffin Cat # 01400 (4-5 lb/bag) and Surgipath embedding >> paraffin? For the manual infiltration: would a beaker of paraffin on a >> 60oC hotplate and stirrer (on low) be OK? >> >> I would be fixing with PFA and decalcidying with 30% EDTA. Might refixing >> in the PFA after decal be a good idea to make sure the tissue integrity is >> maintained? I would probably stored fixed, decalsified samples in 70% EtOH >> until I can process fully. Any one think there would be complications from >> doing this? >> >> Thanks >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade <@t> gmail.com Thu Feb 27 18:17:45 2014 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Thu Feb 27 18:17:53 2014 Subject: [Histonet] manual tissue processing protocol In-Reply-To: <674368B0-A8A2-426B-84C5-8B0D10229C65@yahoo.com> References: <674368B0-A8A2-426B-84C5-8B0D10229C65@yahoo.com> Message-ID: Thanks On Thu, Feb 27, 2014 at 5:50 PM, Kim Donadio wrote: > I agree with Renee here about testing one before. However those are thick > and I'm supposing your fish have scales? This also might slow the process > down. My best guess is you're going to need a much longer processing time. > And since you don't have vacuum I personally think that thickness is going > to need a few hours. I've never processed a fish though take that for what > it's worth. Good luck > > Sent from my iPhone > > On Feb 27, 2014, at 1:15 PM, Rena Fail wrote: > > > Hi, > > > > One mm thick is tiny, 2 mm small 1 cm is rather thick. Since you are > > manually processing run one sample to test your protocol or 2 if you > want > > to see the difference between the shorter method and the longer. > *protocol. > > .Complete dehydration is dependent on the volume, type of tissue and > more > > importantly, the thickness of the tissue. You can check the tissue before > > infiltrating with paraffin.to see if it has > > cleared, If it appears transparent (like a stained glass window.) proceed > > with paraffin infiltration. * > > *Rena Fail* > > > > > > On Wed, Feb 26, 2014 at 2:02 PM, Tyrone Genade > wrote: > > > >> Hello, > >> > >> I need some input on a manual tissue processing protocol I have > received. > >> > >> (5 columns: reagents; tiny to large tissues...) > >> > >> Reagents\embryoes\small tissue\medium tissue\large tissue > >> 70% EtOH\30 min--1hr\1 hr--ON\2hr--ON\4hr--ON > >> 95% EtOH\30 min--1hr\1 hr\1hr\2hr > >> 95% EtOH\30 min\30 min\1 hr\1 hr > >> 100% EtOH\30 min\1 hr\30 min\1 hr > >> 100% EtOH\10 min\30 min\30 min\1 hr > >> toluene\30 min\1 hr\1hr\1 hr > >> toluene\none\30 min\30 min\1 hr till clear > >> parafin infiltration\1hr all > >> parafin infiltration\1 hr\1 hr\2hr\2hr > >> EM400\1 hr\1hr\1hr\2+ hr > >> > >> I would be replacing the toluene with methylbenzoate. Are there any > >> anticipated issues? Is this a good protocol so far as producing good > >> quality tissue for sectioning and analysis? > >> > >> I am not sure what is meant by "small" and "large" tissue. My fish are > >> about 5 cm in length and about 1 cm thick. Would they be classed as > small? > >> > >> Is it necessary using two different types of paraffin : Surgipath > >> infiltrating paraffin Cat # 01400 (4-5 lb/bag) and Surgipath embedding > >> paraffin? For the manual infiltration: would a beaker of paraffin on a > >> 60oC hotplate and stirrer (on low) be OK? > >> > >> I would be fixing with PFA and decalcidying with 30% EDTA. Might > refixing > >> in the PFA after decal be a good idea to make sure the tissue integrity > is > >> maintained? I would probably stored fixed, decalsified samples in 70% > EtOH > >> until I can process fully. Any one think there would be complications > from > >> doing this? > >> > >> Thanks > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@gmail.com tel: +27-84-632-1925 (c) ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From histo <@t> skm.org.pk Fri Feb 28 00:19:06 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Fri Feb 28 00:18:46 2014 Subject: [Histonet] negative control for FLI-1 Message-ID: Dear All, We are looking negative control for FLI-1, as positive control we are using (PNET) on paraffin section successfully. Our pathologist gave us Wilm's tumor and IDC for Negative control but it not work good. Thanks Muhammad Tahseen Sr. Supervisor Histology SKMCH&RC Lahore Pakistan From Haley.Huggins <@t> DignityHealth.org Fri Feb 28 02:53:37 2014 From: Haley.Huggins <@t> DignityHealth.org (Huggins, Haley - MRMC) Date: Fri Feb 28 02:53:51 2014 Subject: [Histonet] RE: Neutralization of AZF In-Reply-To: References: Message-ID: <4F36EC93A5737D4F8A2974E8FB8E2606173EEF32D3@PHX-MSG-007-N2.chw.edu> I would like to know the answer to this too. Haley Huggins Haley Huggins, HT(ASCP)cm Pathology/Histology Manager Marian Medical Center 1400 East Church St Santa Maria, CA 93454 805-739-3170 (path lab) 805-739-3153 (office) 303-652-7453 (cell) 805-332-8697 (rightfax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, February 18, 2014 11:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neutralization of AZF It's been awhile but we have a procedure listed for neutralization of AZF fixative. One of the ingredients is listed as simply sodium carbonate. Does anyone know if this reagent is correct or should it be sodium bicarbonate? We are thinking it should be sodium anhydrous carbonate but can't pull up the original article on the internet. Could some share this information or send me their method of neutralization? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Fri Feb 28 08:12:42 2014 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri Feb 28 08:12:49 2014 Subject: [Histonet] question about refrigeration Message-ID: <731BE09CDB19AA43AA8682199D42D31B1864F07E@ADCEXCHANGE01.KaleidaHealth.org> Histonetters, I was wondering how long a bone specimen (portion of pt's tibia in this case) can be in a refrigerator before it will decompose and not produce a good quality section? The case was completed at 3:30 pm, refrigerated and then put into formalin at 8:15 am the next day? I am pushing to put specimen into formalin immediately after surgery. Thank you. Peggy DiCarlo HT (ASCP) Ortho Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health’s Technology Assistance Center at (716) 859-7777. From trathborne <@t> somerset-healthcare.com Fri Feb 28 08:57:51 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Feb 28 08:58:46 2014 Subject: [Histonet] Slides for control/patient Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95BB1A0@SMCMAIL01.somerset-healthcare.com> Happy Friday! I was wondering, for the labs that are using the slides for IHC that have a place for the control tissue and the patient tissue, which vendor would you recommend. I also would not mind being contacted directly by vendors who wish to inform me of their products. Thanks, Toni From Susan.Walzer <@t> HCAHealthcare.com Fri Feb 28 09:04:44 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Fri Feb 28 09:04:50 2014 Subject: [Histonet] RE: Slides for control/patient In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95BB1A0@SMCMAIL01.somerset-healthcare.com> References: <3AD061FE740D464FAC7BF6B5CFB75707A95BB1A0@SMCMAIL01.somerset-healthcare.com> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FB3B8530B@FWDCWPMSGCMS09.hca.corpad.net> We like Fisher's Red Control Slide with Adhesion #22-042-910 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, February 28, 2014 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides for control/patient Happy Friday! I was wondering, for the labs that are using the slides for IHC that have a place for the control tissue and the patient tissue, which vendor would you recommend. I also would not mind being contacted directly by vendors who wish to inform me of their products. Thanks, Toni _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> commonwealthhealth.net Fri Feb 28 10:00:13 2014 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael J) Date: Fri Feb 28 10:00:25 2014 Subject: [Histonet] Input on Sakura E150 Purchase Message-ID: Another +1 for the VIP E300 (very similar model). We have one that's close to 20 years old that's still running. It has an annual PM and any repairs have been minor. I have been told that part availability isn't yet an issue with this model. Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: margaret chadwell [mailto:m_chadwell@hotmail.com] Sent: Wednesday, February 26, 2014 4:57 PM To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] Input on Sakura E150 Purchase Sent from Windows Mail Hello Histonet, Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it?s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can?t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla, Ca _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From patrick.lewis <@t> seattlechildrens.org Fri Feb 28 11:05:43 2014 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri Feb 28 11:05:54 2014 Subject: [Histonet] Wierd hemotoxylin result on my macaque liver slide Message-ID: <3903BE18914F4440834F0E620415FFCA3823BCF7@PPWEXD01a.childrens.sea.kids> Hi everyone. I was staining a FFPE macaque liver tissue slide with hematoxylin the other day, and it had an odd appearance. All the cells stained the usual way with the blue nuclear staining that you expect with hematoxylin. But a few randomly scattered individual cells throughout the tissue stained a sharp nuclear dark black. The cells are not clustered in any way, the cells have sharp black nuclear staining. It's definitely not artifact. Its not a cluster of lymphoid cells like you would see in inflammation. What would cause seemingly random individual cells scattered throughout the tissue to stain black with just hematoxylin? Just curious Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Ashley.Troutman <@t> Vanderbilt.Edu Fri Feb 28 11:16:58 2014 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Fri Feb 28 11:17:09 2014 Subject: [Histonet] Mouse NK cell marker Message-ID: Good day, colleagues! Does anyone have a good Natural Killer cell marker for mouse tissue? Any antibody recommendations/protocol information would be a big help as well. This is for IHC, not IF. Thanks in advance! Ashley Troutman BS, MBA, HT(ASCP)QIHC Supervisor-Translational Pathology Shared Resource Vanderbilt University Medical Center S-1310 Medical Center North 1161 21st Avenue South Nashville, TN 37232 From smcbride <@t> andrew.cmu.edu Fri Feb 28 12:06:18 2014 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Fri Feb 28 12:06:30 2014 Subject: [Histonet] question about refrigeration In-Reply-To: <731BE09CDB19AA43AA8682199D42D31B1864F07E@ADCEXCHANGE01.KaleidaHealth.org> References: <731BE09CDB19AA43AA8682199D42D31B1864F07E@ADCEXCHANGE01.KaleidaHealth.org> Message-ID: Hi Peggy, In my opinion, it depends upon the objective of the study. Cell autolysis begins shortly after death as a result of lack of oxygen supply as well as nutrients. The membranes of the lysosomes break down, and the acid hydrolases begin to degrade the cellular If you're merely looking to do a macro analysis, such as the quantitation of bone, you should be fine. However, if you're hoping to get results at the cellular level, either with traditional stains or IHC which relies on intact protein structure, you should immerse the specimens immediately after retrieval into formaldehyde solution. Good luck. Best regards, Sean Sean McBride AFIRM Project Leader Senior Scientific Specialist Research Histology Services Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 571-989-BONE (m) 412-268-8275 (o) 412-268-8275 (fax) smcbride@andrew.cmu.edu On Feb 28, 2014, at 9:12 AM, DiCarlo, Margaret wrote: > Histonetters, > > I was wondering how long a bone specimen (portion of pt's tibia in this case) can be in a refrigerator before it will decompose and not produce a good quality section? The case was completed at 3:30 pm, refrigerated and then put into formalin at 8:15 am the next day? I am pushing to put specimen into formalin immediately after surgery. > > Thank you. > > Peggy DiCarlo HT (ASCP) > Ortho Bone Lab > Buffalo General Hospital > Buffalo, NY 14203 > 716-859-1293 > > > The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health?s Technology Assistance Center at (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynn.Burton <@t> Illinois.gov Fri Feb 28 14:27:16 2014 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Fri Feb 28 14:27:34 2014 Subject: [Histonet] Input on Sakura E150 Purchase In-Reply-To: References: Message-ID: We have a Sakura tissue processor that is more than 25 years old and still working very well with annual preventative maintenance. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, February 28, 2014 10:00 AM To: margaret chadwell; histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: RE: [Histonet] Input on Sakura E150 Purchase Another +1 for the VIP E300 (very similar model). We have one that's close to 20 years old that's still running. It has an annual PM and any repairs have been minor. I have been told that part availability isn't yet an issue with this model. Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -----Original Message----- From: margaret chadwell [mailto:m_chadwell@hotmail.com] Sent: Wednesday, February 26, 2014 4:57 PM To: histonet@lists.utsouthwestern.edu; histonet-request@lists.utsouthwestern.edu Subject: [Histonet] Input on Sakura E150 Purchase Sent from Windows Mail Hello Histonet, Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it?s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can?t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla, Ca _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. From Timothy.Morken <@t> ucsfmedctr.org Fri Feb 28 17:31:30 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Feb 28 17:31:42 2014 Subject: [Histonet] herbert skip brown Message-ID: <761E2B5697F795489C8710BCC72141FF3675B825@ex07.net.ucsf.edu> Skip, Please reply to me... Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org From WaitT <@t> livemail.uthscsa.edu Fri Feb 28 22:40:26 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Fri Feb 28 22:40:45 2014 Subject: [Histonet] Bone Histology Protocol Message-ID: <8ddec94ded8f4ad9bae1714af538db09@BL2PR01MB337.prod.exchangelabs.com> Hello colleagues! I'm currently trying to construct a complete Bone Processing Protocol that includes fixation (10%NBF), decalcification (EDTA), Dehydration, Clearing of the decalcificant (Xylene), infiltration, and Embedding in Paraffin. I would like to look at some procedures just to get a good backbone of what a complete procedure is displayed like and I was hoping somebody could give me a website or a source where I could see some. I'm kind of new to bone histological processing so any procedures that are reliable will help! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry