From b-frederick <@t> northwestern.edu Mon Dec 1 08:34:23 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Dec 1 08:35:06 2014 Subject: [Histonet] TMA arrayer Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F1317B0@evcspmbx2.ads.northwestern.edu> Hello all, If anyone has a semi-automated arrayer, where did you get it? We have one from Veridiam, but are wondering if anyone else makes them. We are looking to purchase another unit. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From TanyaAbbott <@t> catholichealth.net Mon Dec 1 12:45:42 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Dec 1 12:46:37 2014 Subject: [Histonet] Competency Assessment Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CD7059D@CHIEX006.CHI.catholichealth.net> Does any have their Surgical Secretaries perform any sort of annual competency? If so, any ideas you could share please? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From Richard.Cartun <@t> hhchealth.org Mon Dec 1 12:53:08 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Dec 1 12:53:14 2014 Subject: [Histonet] Centralizing AP services Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355EF805@HHCEXCHMB03.hhcsystem.org> I would like to connect with individuals who have been involved with the centralization of Anatomic Pathology services within a healthcare system that has multiple partners. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From TanyaAbbott <@t> catholichealth.net Mon Dec 1 13:03:52 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Dec 1 13:06:56 2014 Subject: [Histonet] Processor Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CD705F0@CHIEX006.CHI.catholichealth.net> Looking for a tissue processor to process 200-300 cassettes per run, any favourites? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From Milton.Gomez <@t> nyumc.org Mon Dec 1 13:06:29 2014 From: Milton.Gomez <@t> nyumc.org (Gomez, Milton) Date: Mon Dec 1 13:08:01 2014 Subject: [Histonet] Tissue falling off positive slides In-Reply-To: <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> References: , <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> Message-ID: <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> We bake slides for 30 minutes and tissue does not fall off ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Jamal [j.rowaihi@alborglaboratories.com] Sent: Saturday, November 08, 2014 5:31 AM To: 'Pardue, Judith'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue falling off positive slides Do you use Autostainer? What brand ? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Thursday, November 06, 2014 6:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue falling off positive slides WE are having trouble with tissue coming off our h&e slides. Our heater is set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue positive slides. Judith Pardue CHI Memorial Chatt. Tn. 37343 Judith_pardue@memorial.org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tina.vanmeter <@t> gmail.com Mon Dec 1 13:15:23 2014 From: tina.vanmeter <@t> gmail.com (Tina Van Meter) Date: Mon Dec 1 13:15:27 2014 Subject: [Histonet] Processor In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CD705F0@CHIEX006.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CD705F0@CHIEX006.CHI.catholichealth.net> Message-ID: Sakura VIP 5 or 6? On Mon, Dec 1, 2014 at 2:03 PM, Abbott, Tanya < TanyaAbbott@catholichealth.net> wrote: > Looking for a tissue processor to process 200-300 cassettes per run, any > favourites? > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology > St. Joseph Medical Center > Reading, PA 19603-0316 > ph 610-378-2635 > fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This email and attachments contain information that may be confidential or > privileged. If you are not the intended recipient, notify the sender at > once and delete this message completely from your information system. > Further use, disclosure, or copying of information contained in this email > is not authorized, and any such action should not be construed as a waiver > of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mward <@t> wakehealth.edu Mon Dec 1 13:20:31 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Mon Dec 1 13:20:38 2014 Subject: [Histonet] EDTA decalcification solution In-Reply-To: <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> References: , <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> Message-ID: I am asking this question for our Histology Lab. They are being asked about using EDTA as a decal solution for bone and wondered if anyone else is using this? Is this available as a ready to use? Or do you have to make it up? What vendors are you using and could you provide a procedure about how you use it....length of time in solution, etc. Thanks in advance for your help. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? From hans <@t> histologistics.com Mon Dec 1 13:25:33 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Mon Dec 1 13:25:38 2014 Subject: [Histonet] EDTA decalcification solution In-Reply-To: References: <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> Message-ID: <1ED2CC66-02AA-47F9-B11C-E6CD25E6ECCB@histologistics.com> Hello, We make our own formic acid EDTA for a slow decal solution. Is this what you are looking for? Thanks Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com > On Dec 1, 2014, at 14:20, Martha Ward-Pathology wrote: > > I am asking this question for our Histology Lab. They are being asked about using EDTA as a decal solution for bone and wondered if anyone else is using this? Is this available as a ready to use? Or do you have to make it up? What vendors are you using and could you provide a procedure about how you use it....length of time in solution, etc. > > Thanks in advance for your help. > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 > p 336.716.2109 \ f 336.716.5890 > mward@wakehealth.edu > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans <@t> histologistics.com Mon Dec 1 13:26:56 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Mon Dec 1 13:27:03 2014 Subject: [Histonet] Tissue falling off positive slides In-Reply-To: <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> References: <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> Message-ID: We also bake our slides for at least 30 minutes. Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com > On Dec 1, 2014, at 14:06, Gomez, Milton wrote: > > We bake slides for 30 minutes and tissue does not fall off > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Jamal [j.rowaihi@alborglaboratories.com] > Sent: Saturday, November 08, 2014 5:31 AM > To: 'Pardue, Judith'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Tissue falling off positive slides > > Do you use Autostainer? > What brand ? > > > Best Regards, > > > Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg > Medical Laboratories | Mobile +966 503629832| > j.rowaihi@alborglaboratories.com > Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | > Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | > www.alborglaboratories.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, > Judith > Sent: Thursday, November 06, 2014 6:38 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Tissue falling off positive slides > > WE are having trouble with tissue coming off our h&e slides. Our heater is > set on 65 degrees for 10 minutes. Any suggestions. We use Fisher blue > positive slides. > > Judith Pardue > CHI Memorial > Chatt. Tn. 37343 > Judith_pardue@memorial.org > > This electronic mail and any attached documents are intended solely for the > named addressee(s) and contain confidential information. If you are not an > addressee, or responsible for delivering this email to an addressee, you > have received this email in error and are notified that reading, copying, or > disclosing this email is prohibited. If you received this email in error, > immediately reply to the sender and delete the message completely from your > computer system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Dec 1 14:15:41 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Mon Dec 1 14:15:51 2014 Subject: [Histonet] Centralizing AP services In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355EF805@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E355EF805@HHCEXCHMB03.hhcsystem.org> Message-ID: In that process right now. We have multiple partners, and today started the offical merging/integration of two major health campus AP laboratories( one academic, one non-academic, hospital/clinical), and 3+ smaller sites, outreach, and probably at least two more sites to follow soon. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Richard.Cartun@hhchealth.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 1 Dec 2014 18:53:08 +0000 > Subject: [Histonet] Centralizing AP services > > I would like to connect with individuals who have been involved with the centralization of Anatomic Pathology services within a healthcare system that has multiple partners. Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Mon Dec 1 14:18:01 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Mon Dec 1 14:18:08 2014 Subject: [Histonet] Processor In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF4CD705F0@CHIEX006.CHI.catholichealth.net>, Message-ID: Agree with VIP for conventional tissue processing Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Mon, 1 Dec 2014 14:15:23 -0500 > From: tina.vanmeter@gmail.com > To: TanyaAbbott@catholichealth.net > Subject: Re: [Histonet] Processor > CC: histonet@lists.utsouthwestern.edu > > Sakura VIP 5 or 6? > > On Mon, Dec 1, 2014 at 2:03 PM, Abbott, Tanya < > TanyaAbbott@catholichealth.net> wrote: > > > Looking for a tissue processor to process 200-300 cassettes per run, any > > favourites? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology > > St. Joseph Medical Center > > Reading, PA 19603-0316 > > ph 610-378-2635 > > fax 610-898-5871 > > email: tanyaabbott@catholichealth.net > > > > This email and attachments contain information that may be confidential or > > privileged. If you are not the intended recipient, notify the sender at > > once and delete this message completely from your information system. > > Further use, disclosure, or copying of information contained in this email > > is not authorized, and any such action should not be construed as a waiver > > of privilege or other confidentiality protections. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dunatrsd <@t> sbcglobal.net Mon Dec 1 15:04:06 2014 From: dunatrsd <@t> sbcglobal.net (dusko trajkovic) Date: Mon Dec 1 15:06:51 2014 Subject: [Histonet] MUC1 antibody inquiry Message-ID: <1417467846.5121.YahooMailNeo@web181706.mail.ne1.yahoo.com> Hallo Everyone, I am looking for information on MUC1 antibody that is known to react only to Human, and does not cross-react with the mouse for FFPE IHC procedure. Any suggestions/advice will be greatly appreciated. Thank you and have a good Monday!! Dusko From lbest <@t> sunriselab.com Tue Dec 2 05:35:32 2014 From: lbest <@t> sunriselab.com (Laurie Best) Date: Tue Dec 2 05:35:45 2014 Subject: [Histonet] Decolorizing effect on IHC stain Message-ID: <68C2805BA426E24AB117C295AC50C901628DDD51@NY-SML-EMAIL-01.sunriselab.com> Is there any information available that would indicate the effect of decolorizing an H&E stained slide on subsequent IHC staining / antigenicity? Thanks, Laurie Laurie Best,BS Histology and Cyto Prep Supervisor Sunrise Medical Laboratories 250 Miller Place Hicksville, N.Y. 11801 tel (631)435-1515x1018 fax(631)435-2014 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." From veronique.barres <@t> gmail.com Tue Dec 2 07:31:53 2014 From: veronique.barres <@t> gmail.com (=?UTF-8?B?VsOpcm9uaXF1ZSBCYXJyw6hz?=) Date: Tue Dec 2 07:32:41 2014 Subject: [Histonet] Re: TMA arrayer Message-ID: Hi Bernice, We have the TMArrayer from Pathology Devices and we love it. Easy to use, we had no problem with it since 3 years and built quite a lot of TMAs. V?ronique From Ashley.Troutman <@t> Vanderbilt.Edu Tue Dec 2 07:54:26 2014 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Tue Dec 2 07:55:09 2014 Subject: [Histonet] TMA arrayer Message-ID: Hello Bernice, We have a TMA Grand Master from Perkin Elmer. It is quite a machine. Let me know by email if you would like contact information. Regards, Ashley Troutman BS, MBA, HT(ASCP)QIHC Supervisor-Translational Pathology Shared Resource Vanderbilt University Medical Center S-1310 Medical Center North 1161 21st Avenue South Nashville, TN 37232 Message: 1 Date: Mon, 1 Dec 2014 14:34:23 +0000 From: Bernice Frederick > Subject: [Histonet] TMA arrayer To: "Histonet (histonet@lists.utsouthwestern.edu)" > Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F1317B0@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Hello all, If anyone has a semi-automated arrayer, where did you get it? We have one from Veridiam, but are wondering if anyone else makes them. We are looking to purchase another unit. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu> From rjbuesa <@t> yahoo.com Tue Dec 2 08:29:32 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 2 08:29:37 2014 Subject: [Histonet] Decolorizing effect on IHC stain In-Reply-To: <68C2805BA426E24AB117C295AC50C901628DDD51@NY-SML-EMAIL-01.sunriselab.com> References: <68C2805BA426E24AB117C295AC50C901628DDD51@NY-SML-EMAIL-01.sunriselab.com> Message-ID: <51827653.2326530.1417530572801.JavaMail.yahoo@jws100201.mail.ne1.yahoo.com> You can decolorize an H&E stain and use the decolorized section for IHC procedure. Ren? J.? On Tuesday, December 2, 2014 6:36 AM, Laurie Best wrote: Is there any information available that would indicate the effect of decolorizing an H&E stained slide on subsequent IHC staining / antigenicity? Thanks, Laurie Laurie Best,BS Histology and Cyto Prep Supervisor Sunrise Medical Laboratories 250 Miller Place Hicksville, N.Y. 11801 tel (631)435-1515x1018 fax(631)435-2014 "This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Finlay.Finlay <@t> glasgow.ac.uk Tue Dec 2 08:41:52 2014 From: Finlay.Finlay <@t> glasgow.ac.uk (Finlay Finlay) Date: Tue Dec 2 08:39:56 2014 Subject: [Histonet] mortuary scales advice Message-ID: <0164C4117A8D594DBBF5265626928E39093B22@FORMED-ES.formed.internal> Hello all, I am searching for a weighing scale that will record multiple organ weights during autopsy and then allow display of all data at the end of the session. Ideally this would be a stand-alone unit rather than computer linked. Does anyone have experience with this sort of equipment? Thank you Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay@glasgow.ac.uk The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. From akbitting <@t> geisinger.edu Tue Dec 2 08:53:01 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Dec 2 08:53:22 2014 Subject: [Histonet] TMA arrayer In-Reply-To: References: Message-ID: We also have the Grand Master. It's quite amazing, however we have had issues with the punches occasionally. Also alignment issues which turned out to be software related. Have you seen any of this Ashley?? Regards, Angie Sent from my iPhone > On Dec 2, 2014, at 8:59 AM, Troutman, Kenneth A wrote: > > Hello Bernice, > > We have a TMA Grand Master from Perkin Elmer. It is quite a machine. Let me know by email if you would like contact information. > > Regards, > > Ashley Troutman BS, MBA, HT(ASCP)QIHC > Supervisor-Translational Pathology Shared Resource > Vanderbilt University Medical Center > S-1310 Medical Center North > 1161 21st Avenue South > Nashville, TN 37232 > > Message: 1 > Date: Mon, 1 Dec 2014 14:34:23 +0000 > From: Bernice Frederick > > Subject: [Histonet] TMA arrayer > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > > Message-ID: > <62C639732D3F274DACED033EBDF6ADAF2F1317B0@evcspmbx2.ads.northwestern.edu> > > Content-Type: text/plain; charset="us-ascii" > > Hello all, > If anyone has a semi-automated arrayer, where did you get it? We have one from Veridiam, but are wondering if anyone else makes them. We are looking to purchase another unit. > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu> > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From tina.vanmeter <@t> gmail.com Tue Dec 2 09:02:32 2014 From: tina.vanmeter <@t> gmail.com (Tina Van Meter) Date: Tue Dec 2 09:02:38 2014 Subject: [Histonet] Processor In-Reply-To: <547d76b4.d1a9420a.3485.5455SMTPIN_ADDED_BROKEN@mx.google.com> References: <852F7D2C14FB464D80E182B15DB138AF4CD705F0@CHIEX006.CHI.catholichealth.net> <547d76b4.d1a9420a.3485.5455SMTPIN_ADDED_BROKEN@mx.google.com> Message-ID: ?I beg to differ....The VIP 5 is capable of processing 300 cassettes. On the first page of my VIP 5, Operating Manual (p. 1.1), it says it is capable of handling 150-300 cassettes. Tina Van Meter Histology Core Manager Scripps Research Institute Jupiter, FL On Tue, Dec 2, 2014 at 3:22 AM, Jamal wrote: > Hi > Sakura VIP 5 dose not process more than 150 cassettes per day > > I recommend Sakura VIP 6 or Leica ASP 6025 > > > Best Regards, > > > Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg > Medical Laboratories | Mobile +966 503629832| > j.rowaihi@alborglaboratories.com > Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | > Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | > www.alborglaboratories.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tina Van Meter > Sent: Monday, December 01, 2014 10:15 PM > To: Abbott, Tanya > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Processor > > Sakura VIP 5 or 6? > > On Mon, Dec 1, 2014 at 2:03 PM, Abbott, Tanya < > TanyaAbbott@catholichealth.net> wrote: > > > Looking for a tissue processor to process 200-300 cassettes per run, any > > favourites? > > > > Tanya G. Abbott RT (CSMLS) > > Manager Technologist, Histology/Cytology > > St. Joseph Medical Center > > Reading, PA 19603-0316 > > ph 610-378-2635 > > fax 610-898-5871 > > email: tanyaabbott@catholichealth.net > > > > This email and attachments contain information that may be confidential > or > > privileged. If you are not the intended recipient, notify the sender at > > once and delete this message completely from your information system. > > Further use, disclosure, or copying of information contained in this > email > > is not authorized, and any such action should not be construed as a > waiver > > of privilege or other confidentiality protections. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From TanyaAbbott <@t> catholichealth.net Tue Dec 2 09:29:25 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Tue Dec 2 09:29:51 2014 Subject: [Histonet] Water baths Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From akemiat3377 <@t> gmail.com Tue Dec 2 09:46:38 2014 From: akemiat3377 <@t> gmail.com (Eileen Akemi Allison) Date: Tue Dec 2 09:46:43 2014 Subject: [Histonet] Water baths In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> Message-ID: <8B20C7DC-39B0-4305-BEEE-24D25D9ECDA2@gmail.com> Speaking for myself and the labs I have managed, I have been checking ALL temps and recording them since the early 90?s. These include waters baths for tissue flotation, and ones used for special stains, ovens, refrigerators, freezers, cryostats, tissue processors, embedding centers, decloaking chambers and room temps. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aallison@montereygi.com Tele: (831) 375-3577 X117 > On Dec 2, 2014, at 7:29 AM, Abbott, Tanya wrote: > > Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology > St. Joseph Medical Center > Reading, PA 19603-0316 > ph 610-378-2635 > fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Tue Dec 2 09:47:48 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Tue Dec 2 09:47:58 2014 Subject: [Histonet] back orders from various vendors In-Reply-To: References: , <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> Message-ID: I just need a moment to vent....is it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not available....expected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems ..... Thanks for letting get this off my chest.....I will resume my frantic search for another antibody vendor now..... Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MAUGER <@t> email.chop.edu Tue Dec 2 09:55:18 2014 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Tue Dec 2 09:55:57 2014 Subject: [Histonet] RE: back orders from various vendors In-Reply-To: References: , <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> Message-ID: <929548F049BC6D448DF9488B556449BE1659068A@EXCMBXPW5.chop.edu> Martha, Yes, as a matter of fact I have been noticing this too! A real pain. Jo Mauger -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to vent....is it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not available....expected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems ..... Thanks for letting get this off my chest.....I will resume my frantic search for another antibody vendor now..... Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Tue Dec 2 09:56:06 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Dec 2 09:56:11 2014 Subject: [Histonet] RE: Water baths In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> Message-ID: <12ECD7346266D74691EC2BFC75285E453814A847@BFL323E10.pathmdlabs.local> Yes, I have always done that at any lab I worked at. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 7:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Dec 2 09:59:22 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Dec 2 09:59:36 2014 Subject: [Histonet] RE: back orders from various vendors In-Reply-To: References: , <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> Message-ID: <49c66922c51b484aa8ffe019e0913aec@MAIL13M2N2.ad.uams.edu> Happens every year at this time with various vendors and end of year. I always over order in October to be sure I am good through the end of the year or as close as I can. I know the things I use most and those are just what is always in backorder at the end of the year. October became my answer as long as expiration dates are not an issue. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to vent....is it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not available....expected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems ..... Thanks for letting get this off my chest.....I will resume my frantic search for another antibody vendor now..... Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mundayscott <@t> gmavt.net Tue Dec 2 10:00:15 2014 From: mundayscott <@t> gmavt.net (scott munday) Date: Tue Dec 2 10:00:18 2014 Subject: [Histonet] Microscope Problems? Message-ID: Is your lab in need of a new microscope or replacement power supply? We have several Olympus BX40 and BX41 compound microscopes. Also, we offer a solution to the broken rheostat (light switch) problem that is common with the BX40. Please Email or call with questions regarding Olympus Microscope issues. -- Scott Munday Phone: 919-775-5596 Fax: 919-776-9566 From lcolbert <@t> pathmdlabs.com Tue Dec 2 10:00:44 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Dec 2 10:00:53 2014 Subject: [Histonet] RE: back orders from various vendors In-Reply-To: References: , <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> Message-ID: <12ECD7346266D74691EC2BFC75285E453814A88E@BFL323E10.pathmdlabs.local> Yes, I am having this issue with one vendor in particular (which I won't mention). It has affected our operations and it is very frustrating! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to vent....is it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not available....expected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems ..... Thanks for letting get this off my chest.....I will resume my frantic search for another antibody vendor now..... Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ryan.Roy <@t> va.gov Tue Dec 2 10:14:13 2014 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Tue Dec 2 10:17:47 2014 Subject: [EXTERNAL] Re: [Histonet] Water baths In-Reply-To: <8B20C7DC-39B0-4305-BEEE-24D25D9ECDA2@gmail.com> References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> <8B20C7DC-39B0-4305-BEEE-24D25D9ECDA2@gmail.com> Message-ID: <15F883394EAB744E99E1C7E1B987304901770400213D@R04BYNMSGB1.r04.med.va.gov> Hello, I agree, we record daily all our temperatures for all equipment in histology. Ryan Roy HTL (ASCP)cm Veterans Affairs Medical Center Manchester NH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Tuesday, December 02, 2014 10:47 AM To: Abbott, Tanya Cc: Histonet Subject: [EXTERNAL] Re: [Histonet] Water baths Speaking for myself and the labs I have managed, I have been checking ALL temps and recording them since the early 90?s. These include waters baths for tissue flotation, and ones used for special stains, ovens, refrigerators, freezers, cryostats, tissue processors, embedding centers, decloaking chambers and room temps. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aallison@montereygi.com Tele: (831) 375-3577 X117 > On Dec 2, 2014, at 7:29 AM, Abbott, Tanya > wrote: > > Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology St. Joseph Medical Center > Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 > email: tanyaabbott@catholichealth.net > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Tue Dec 2 10:17:50 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Tue Dec 2 10:17:54 2014 Subject: [Histonet] RE: back orders from various vendors In-Reply-To: <49c66922c51b484aa8ffe019e0913aec@MAIL13M2N2.ad.uams.edu> References: , <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> <49c66922c51b484aa8ffe019e0913aec@MAIL13M2N2.ad.uams.edu> Message-ID: At least I know I am not crazy or being paranoid! :) this year just seems to be worse than I ever remember it being....and the worse part is while I am freaking out because I can't get something I desperately need, the customer service person can only say "We are sorry for the inconvenience". Argggggg!!!! -----Original Message----- From: Marcum, Pamela A [mailto:PAMarcum@uams.edu] Sent: Tuesday, December 02, 2014 10:59 AM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: back orders from various vendors Happens every year at this time with various vendors and end of year. I always over order in October to be sure I am good through the end of the year or as close as I can. I know the things I use most and those are just what is always in backorder at the end of the year. October became my answer as long as expiration dates are not an issue. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, December 02, 2014 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back orders from various vendors I just need a moment to vent....is it just me or is everyone seeing more and more back order issues with their various vendors? It is not specific to any one particular vendor, product or even category of product, but lately I am being told more and more often that an item is not available....expected release dates anywhere from a few days to 6-8 weeks! This is starting to cause real problems ..... Thanks for letting get this off my chest.....I will resume my frantic search for another antibody vendor now..... Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Joyce.Weems <@t> emoryhealthcare.org Tue Dec 2 10:19:37 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Dec 2 10:20:52 2014 Subject: [Histonet] RE: Water baths In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> Message-ID: It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Valerie.Hannen <@t> parrishmed.com Tue Dec 2 10:49:30 2014 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Dec 2 10:50:19 2014 Subject: [Histonet] RE: Water baths In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> Message-ID: <450B7A81EDA0C54E97C53D60F00776C323373841E8@isexstore03> We still do, due to the fact that it is on our general "temperature" chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From Toni.Rathborne <@t> rwjuh.edu Tue Dec 2 11:04:57 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Tue Dec 2 11:05:04 2014 Subject: [Histonet] RE: Water baths In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C323373841E8@isexstore03> References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> <450B7A81EDA0C54E97C53D60F00776C323373841E8@isexstore03> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24EDF477@vap1014.win.rwjuh.edu> In the All Common checklist, COM.30750, a Phase II, addresses this and it is a requirement. "Temperatures are checked and recorded each day of use for all temperature-dependent equipment and environments using a calibrated thermometer." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 11:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general "temperature" chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Tue Dec 2 11:07:54 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Tue Dec 2 11:08:00 2014 Subject: [Histonet] RE: EDTA decalcification solution follow up questions In-Reply-To: References: , <60e5644d-b465-424f-bf4c-d991f18228d7@MSGWCDCPHT67.nyumc.org> <2B0D905D71C9AA4B859B99F404A6C4A11116D6E8@MSGWCDCPMB27.nyumc.org> Message-ID: Thank you to everyone who has responded. They have a more specific question: Currently they are using SurgiPath DeCal solution II. They are being asked to use an EDTA decal solution that will provide the best preservation of RNA and DNA proteins. What is everyone using for this purpose? Thanks again for any information.... ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Monday, December 01, 2014 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EDTA decalcification solution I am asking this question for our Histology Lab. They are being asked about using EDTA as a decal solution for bone and wondered if anyone else is using this? Is this available as a ready to use? Or do you have to make it up? What vendors are you using and could you provide a procedure about how you use it....length of time in solution, etc. Thanks in advance for your help. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pmcdavid <@t> mhg.com Tue Dec 2 11:18:59 2014 From: Pmcdavid <@t> mhg.com (Patti McDavid) Date: Tue Dec 2 11:19:04 2014 Subject: [Histonet] Water Baths Message-ID: <1F886A89F4A53345928A11203C028957DAA6DC8A@JANDC600-MBN01.mhg.local> We record daily temperatures of water baths used for cutting. Patti L. McDavid Laboratory Manager 4500 Thirteenth Street P.O. Box 1810 Gulfport, MS 39502-1810 Phone 228-575-2340 Fax 228-865-3325 Pmcdavid@mhg.com [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **********PRIVATE AND CONFIDENTIAL********** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. From Pmcdavid <@t> mhg.com Tue Dec 2 11:20:50 2014 From: Pmcdavid <@t> mhg.com (Patti McDavid) Date: Tue Dec 2 11:21:06 2014 Subject: [Histonet] Tissue Processor Message-ID: <1F886A89F4A53345928A11203C028957DAA6DC9F@JANDC600-MBN01.mhg.local> We have used VIP in the past and it was adequate. We use the Leica Peloris as our main processor and love it. Patti L. McDavid Laboratory Manager 4500 Thirteenth Street P.O. Box 1810 Gulfport, MS 39502-1810 Phone 228-575-2340 Fax 228-865-3325 Pmcdavid@mhg.com [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **********PRIVATE AND CONFIDENTIAL********** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. From mpence <@t> grhs.net Tue Dec 2 11:22:36 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Dec 2 11:22:55 2014 Subject: [Histonet] RE: Tissue Processor In-Reply-To: <1F886A89F4A53345928A11203C028957DAA6DC9F@JANDC600-MBN01.mhg.local> References: <1F886A89F4A53345928A11203C028957DAA6DC9F@JANDC600-MBN01.mhg.local> Message-ID: Same thing here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti McDavid Sent: Tuesday, December 02, 2014 11:22 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue Processor We have used VIP in the past and it was adequate. We use the Leica Peloris as our main processor and love it. Patti L. McDavid Laboratory Manager 4500 Thirteenth Street P.O. Box 1810 Gulfport, MS 39502-1810 Phone 228-575-2340 Fax 228-865-3325 Pmcdavid@mhg.com [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **********PRIVATE AND CONFIDENTIAL********** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. From Maxim_71 <@t> mail.ru Tue Dec 2 11:46:48 2014 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Tue Dec 2 11:50:28 2014 Subject: [Histonet] Decolorizing effect on IHC stain Message-ID: <1429577540.20141202204648@mail.ru> Laurie, The slides must be pre-coated with poly-L-lisine or any other type adhesive, suitable for IHC. IHC is done usually for a several markers. Do you have a several HE slides? Sincerely, Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru From tbraud <@t> holyredeemer.com Tue Dec 2 12:48:33 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Dec 2 12:49:39 2014 Subject: [Histonet] RE: Surgical Secretary Compentancy In-Reply-To: <20141202145058.62EDC1E981B@trendmess-svr.holyredeemer.local> References: <20141202145058.62EDC1E981B@trendmess-svr.holyredeemer.local> Message-ID: Surgical Secretary Compentancy - Funny you should mention this as I had just finished a review containing just such a competency, just before reading this. Our Surgical Secretaries take an annual typing test for word speed, have to demonstrate knowledge of certain computer functions, proficiency at using certain LIS reporting functions, meeting typing and send out TAT and also a few basic office items, such as answering the phone correctly and billing of miscellaneous send out tests. I hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- Sent: Tuesday, December 02, 2014 9:51 AM Subject: Histonet Digest, Vol 133, Issue 2 Today's Topics: 1. Competency Assessment (Abbott, Tanya) Does any have their Surgical Secretaries perform any sort of annual competency? If so, any ideas you could share please? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Steven.Swartwood <@t> cshs.org Tue Dec 2 13:37:51 2014 From: Steven.Swartwood <@t> cshs.org (Swartwood, Steven J) Date: Tue Dec 2 13:38:02 2014 Subject: [Histonet] FoxP3 and Geminin Immunohistochemistry Message-ID: <959202AC61AEF942968646EC66E2BE3E3BF09D6D@ESPWMSGMBX07.CSMC.EDU> Hello all, I've done some research on Foxp3 and Geminin and I've seen a few antibodies that look sufficient for IHC in FFPE tissues. My lab is currently looking into purchasing these antibodies for a few different projects. Does anyone on here use these two antibodies for IHC in FFPE tissues? I'm going to use them in bright field, but we may also turn to IF for other studies. I'd be grateful for any information from what clone/company do you buy from and retrieval methods to primary antibody incubations/dilutions. Thank you all for any information, Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartwood@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. From jburch01 <@t> gmail.com Tue Dec 2 14:13:09 2014 From: jburch01 <@t> gmail.com (Jim Burchette) Date: Tue Dec 2 14:13:13 2014 Subject: [Histonet] Decolorizing effect on IHC stain In-Reply-To: <68C2805BA426E24AB117C295AC50C901628DDD51@NY-SML-EMAIL-01.sunriselab.com> References: <68C2805BA426E24AB117C295AC50C901628DDD51@NY-SML-EMAIL-01.sunriselab.com> Message-ID: I suggest you run two positive controls. One control stained with H&E the decolorized. The other as usual. JB On Dec 2, 2014 3:35 AM, "Laurie Best" wrote: > Is there any information available that would indicate the effect of > decolorizing an H&E stained slide on subsequent IHC staining / antigenicity? > Thanks, Laurie > > > Laurie Best,BS > Histology and Cyto Prep Supervisor > Sunrise Medical Laboratories > 250 Miller Place > Hicksville, N.Y. 11801 > tel (631)435-1515x1018 > fax(631)435-2014 > > "This message contains privileged and confidential information intended > only for the use of the addressee named above. If you are not the intended > recipient of this message you must not disseminate, copy or take any action > in reliance on it." > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From chapmanc <@t> health.missouri.edu Tue Dec 2 15:22:38 2014 From: chapmanc <@t> health.missouri.edu (Chapman, Cherie J.) Date: Tue Dec 2 15:22:44 2014 Subject: [Histonet] (no subject) Message-ID: Does anyone know if you have to use controls for IHC on MOHS frozen section procedures to satisfy CAP requirements? Thank you, Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 From pruegghm <@t> hotmail.com Tue Dec 2 15:32:01 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Tue Dec 2 15:32:05 2014 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: I would think you would have to validate IHC for MOHS the same as FFPE, once the validation process is done maybe controls would not be necessary. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net > From: chapmanc@health.missouri.edu > To: histonet@lists.utsouthwestern.edu > Date: Tue, 2 Dec 2014 21:22:38 +0000 > Subject: [Histonet] (no subject) > > Does anyone know if you have to use controls for IHC on MOHS frozen section procedures to satisfy CAP requirements? > > Thank you, > > Cherie Chapman, BS, HT, HTL (ASCP) > Associate Director of Dermatopathology Laboratory > University of Missouri Department of Dermatology > University Physicians Medical Building > Phone: (573) 884-0123 > Fax: (573) 884-0834 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Tue Dec 2 16:48:18 2014 From: suetp918 <@t> comcast.net (suetp918) Date: Tue Dec 2 16:48:48 2014 Subject: [Histonet] Water baths Message-ID: To be honest we take every temp but that. ?Really do not see the need Stp TJUH Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: "Abbott, Tanya" Date:12/02/2014 10:29 AM (GMT-05:00) To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> skm.org.pk Tue Dec 2 23:16:09 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Tue Dec 2 23:16:37 2014 Subject: [Histonet] RE: Water baths In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> Message-ID: We have been checking ALL temps and recording every shift. These include cryostats, Embedding centers, Wax dispenser, Hot plates, Hot Ovens, Body Refrigerator, refrigerators, Water Baths, and room temps and Humidity%. Muhammad Tahseen MLT (PMF), MT (JIMTEF) Japan Senior supervisor histopathology SKMCH&RC Lahore Pakistan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 8:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 3 08:41:24 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 3 08:41:29 2014 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <1981060267.2697200.1417617684553.JavaMail.yahoo@jws10070.mail.ne1.yahoo.com> Yes, you have to. The origin of the section (MOHS or other) is not the criteria to use controls, but the process is.Ren? J.? On Tuesday, December 2, 2014 4:23 PM, "Chapman, Cherie J." wrote: Does anyone know if you have to use controls for IHC on MOHS frozen section procedures to satisfy CAP requirements? Thank you, Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From timma <@t> dxandtx.com Wed Dec 3 10:09:20 2014 From: timma <@t> dxandtx.com (Timm, Amber L) Date: Wed Dec 3 10:12:06 2014 Subject: [Histonet] RE: Water baths In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C323373841E8@isexstore03> References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> <450B7A81EDA0C54E97C53D60F00776C323373841E8@isexstore03> Message-ID: What is everyone using as their histo refrigerator temperature range? Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic & Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: timma@dxandtx.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 10:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general "temperature" chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Toni.Rathborne <@t> rwjuh.edu Wed Dec 3 10:22:23 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Wed Dec 3 10:22:38 2014 Subject: [Histonet] RE: Water baths In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> <450B7A81EDA0C54E97C53D60F00776C323373841E8@isexstore03> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24EDF9C8@vap1014.win.rwjuh.edu> We use whatever is the narrowest range for the reagents stored in there. So if there is one reagent with a range of 4-10?C, and another with 2-8?C, we'll use 4-8? as our range. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timm, Amber L Sent: Wednesday, December 03, 2014 11:09 AM To: Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths What is everyone using as their histo refrigerator temperature range? Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic & Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: timma@dxandtx.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 10:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general "temperature" chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Wed Dec 3 10:25:15 2014 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Dec 3 10:25:54 2014 Subject: [Histonet] RE: Water baths In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> <450B7A81EDA0C54E97C53D60F00776C323373841E8@isexstore03> Message-ID: We use the range 2C to 8C - the range published for products requiring refrigeration in the Sigma catalog. tg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timm, Amber L Sent: Wednesday, December 03, 2014 9:09 AM To: Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths What is everyone using as their histo refrigerator temperature range? Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic & Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: timma@dxandtx.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 10:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general "temperature" chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From areichard <@t> lmhealth.org Wed Dec 3 10:26:53 2014 From: areichard <@t> lmhealth.org (Amanda Reichard) Date: Wed Dec 3 10:27:02 2014 Subject: [Histonet] Non-GYN Prep Safety Message-ID: Question for you all Do you process Non-GYN specimens under a hood? What type of precautions are taken for suspect mycobacterium specimens? Are your ThinPrep machines under a hood as well? Thanks in advance Amanda Reichard, HTL (ASCP)cm Histotechnologist Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From timma <@t> dxandtx.com Wed Dec 3 10:27:00 2014 From: timma <@t> dxandtx.com (Timm, Amber L) Date: Wed Dec 3 10:27:25 2014 Subject: [Histonet] RE: Water baths In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF4CD71ED0@CHIEX006.CHI.catholichealth.net> <450B7A81EDA0C54E97C53D60F00776C323373841E8@isexstore03> Message-ID: Thank you all I was looking to verify the tissue storage temp. that we have. Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic & Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: timma@dxandtx.com -----Original Message----- From: Goins, Tresa [mailto:TGoins@mt.gov] Sent: Wednesday, December 03, 2014 10:25 AM To: Timm, Amber L; Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: RE: Water baths We use the range 2C to 8C - the range published for products requiring refrigeration in the Sigma catalog. tg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timm, Amber L Sent: Wednesday, December 03, 2014 9:09 AM To: Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths What is everyone using as their histo refrigerator temperature range? Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic & Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: timma@dxandtx.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 10:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general "temperature" chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From lcolbert <@t> pathmdlabs.com Wed Dec 3 10:49:25 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Dec 3 10:49:33 2014 Subject: [Histonet] Prostate Needle Bx Message-ID: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. Laurie Colbert From POWELL_SA <@t> mercer.edu Wed Dec 3 10:57:23 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Dec 3 10:57:38 2014 Subject: [Histonet] RE: Prostate Needle Bx In-Reply-To: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> Message-ID: <9BF995BC0E47744E9673A41486E24EE25C006C0264@MERCERMAIL.MercerU.local> I have discovered a wonderful cassette from Cancer Diagnostics, it is called the Autocassette and has an oval concave cavity with no corners for small pieces of tissue to get caught in and the solutions pass through it very nicely. I use it for small research samples, like shrimp gills similar in size to the prostate biopsies, and I lose nothing. I use the yellow ones, VB1005 but they have other colors and also some with smaller grid. Highly recommend them. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, December 03, 2014 11:49 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Prostate Needle Bx How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Dec 3 11:09:02 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Dec 3 11:09:33 2014 Subject: [Histonet] RE: Non-GYN Prep Safety In-Reply-To: References: Message-ID: Specimen prep under a hood - universal precautions - ThinPrep not under a hood. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Reichard Sent: Wednesday, December 03, 2014 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Non-GYN Prep Safety Question for you all Do you process Non-GYN specimens under a hood? What type of precautions are taken for suspect mycobacterium specimens? Are your ThinPrep machines under a hood as well? Thanks in advance Amanda Reichard, HTL (ASCP)cm Histotechnologist Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From HernandezJW <@t> uthscsa.edu Wed Dec 3 11:23:31 2014 From: HernandezJW <@t> uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Wed Dec 3 11:23:37 2014 Subject: [Histonet] MMA Mounting Issue with Haupts Adhesive Message-ID: Hi all, I had a question about how to properly mount MMA sectioned slides using Haupt's (Dorn and Hart Microedge). My protocol is as follows: 1. Stick Glass Slide into 50mL Falcon Tube with Haupts solution (1:1 50% EtOH:50% Haupts). 2. Rotate the tube to ensure entire coating of glass slide. 3. Let glass slide dry in fume hood for about 10minutes. 4. Remove cut microtome sections (range: 50-5microns). 5. Place cut sections in 70%EtOH to soften the plastic and unroll. (sections are coming out rolled up like scrolls) 6. Remove a section from the 70%EtOH using forceps and try unrolling it with a needle on a glass slide. 7. Once the section(s) is stretched on the coated glass slide, cover with saran wrap. 8. Cover the wrapped slide with coarse filter paper and use a small seam roller to roll the sections on the glass slide as flat as possible. This will also help remove excess 70%EtOH. 9. Remove the filter paper and stack glass slides in a slide press or similar device. Place tongue depressors or small pieces of wood equivalent to the slide length that are about 1cm thick at the end of the glass slide stack. 10. Let slides dry over night at room temperature in press. 11. Remove the slides from the press and unwrap the saran wrap to expose your adhered sample. Questions/Comments: 1. When I pull off the saran wrap, the sample sometimes comes off. The plastic in the sample turns white and dries out and does not attach. This is seen in the majority of my tissue sections. 2. The only time I have noticed consistent attachment is for a 5micron sized section. 3. How can I adjust the microtome so that the samples do not come off so scroll-like? My cutting angle is typically either 0 or 2.5 degrees. I also apply 70% EtOH to the block surface to soften the plastic. Overall goal: Get the tissue sections adhered to the glass slides so that I can proceed with staining. Thank you all. Regards, Jesse Hernandez From tpodawiltz <@t> lrgh.org Wed Dec 3 11:24:58 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Dec 3 11:25:11 2014 Subject: [Histonet] RE: Non-GYN Prep Safety In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D9863863261613717F@LRGHEXVS1.practice.lrgh.org> Unfixed specimen should be done under a biological safety cabinet. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Reichard Sent: Wednesday, December 03, 2014 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Non-GYN Prep Safety Question for you all Do you process Non-GYN specimens under a hood? What type of precautions are taken for suspect mycobacterium specimens? Are your ThinPrep machines under a hood as well? Thanks in advance Amanda Reichard, HTL (ASCP)cm Histotechnologist Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From kmilne <@t> bccrc.ca Wed Dec 3 11:34:34 2014 From: kmilne <@t> bccrc.ca (Katy Milne) Date: Wed Dec 3 11:34:40 2014 Subject: [Histonet] RE: FoxP3 and Geminin Immunohistochemistry In-Reply-To: <20141203172721.E58894994B_47F47F9F@vmaprod6.mail-relay.ubc.ca> References: <20141203172721.E58894994B_47F47F9F@vmaprod6.mail-relay.ubc.ca> Message-ID: Hi Steven, I can't speak to geminin but I have use the following 2 clones of FoxP3 with success (in brightfield) Spring Biosciences clone SP97 (rbMAb) Ebioscience Clone ebio-7979 (msMAb) For both I use the Diva decloaking solution from Biocare so pretty much standard citrate antigen retrieval should get you there. I'm assuming you're talking about human tissue, otherwise I have another one I use on mouse tissue. Katy Milne Deeley Research Centre -----Original Message----- Message: 2 Date: Tue, 2 Dec 2014 19:37:51 +0000 From: "Swartwood, Steven J" Subject: [Histonet] FoxP3 and Geminin Immunohistochemistry To: "histonet@lists.utsouthwestern.edu" Message-ID: <959202AC61AEF942968646EC66E2BE3E3BF09D6D@ESPWMSGMBX07.CSMC.EDU> Content-Type: text/plain; charset="us-ascii" Hello all, I've done some research on Foxp3 and Geminin and I've seen a few antibodies that look sufficient for IHC in FFPE tissues. My lab is currently looking into purchasing these antibodies for a few different projects. Does anyone on here use these two antibodies for IHC in FFPE tissues? I'm going to use them in bright field, but we may also turn to IF for other studies. I'd be grateful for any information from what clone/company do you buy from and retrieval methods to primary antibody incubations/dilutions. Thank you all for any information, Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartwood@cshs.org From Ashley.Troutman <@t> Vanderbilt.Edu Wed Dec 3 12:02:38 2014 From: Ashley.Troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Wed Dec 3 12:03:42 2014 Subject: [Histonet] FoxP3 and Geminin Immunohistochemistry Message-ID: Hello Steven, I have used both of these antibodies for various projects and we performed all of these on the Bondmax from Leica with a 60 minute antibody incubation. We have used two FoxP3 antibodies from eBiosciences; cat# 14-4777 on human tissue and cat# 13-5773 for mouse tissue. (both were 1:100 dilutions and ER2 for 20 mins (EDTA pH9). I have only used Geminin on human tissue. It was from Proteintech Group cat# 10802-1-AP (1:1500 dilution using ER1 for 15 min-ER1=citrate pH6) All of these were on FFPE tissues for brightfield only. We did not use them for IF. If you need any other specifics, let me know. Ashley Troutman BS, MBA, HT(ASCP)QIHC Supervisor-Translational Pathology Shared Resource Vanderbilt University Medical Center S-1310 Medical Center North 1161 21st Avenue South Nashville, TN 37232 Message: 2 Date: Tue, 2 Dec 2014 19:37:51 +0000 From: "Swartwood, Steven J" > Subject: [Histonet] FoxP3 and Geminin Immunohistochemistry To: "histonet@lists.utsouthwestern.edu" > Message-ID: <959202AC61AEF942968646EC66E2BE3E3BF09D6D@ESPWMSGMBX07.CSMC.EDU> Content-Type: text/plain; charset="us-ascii" Hello all, I've done some research on Foxp3 and Geminin and I've seen a few antibodies that look sufficient for IHC in FFPE tissues. My lab is currently looking into purchasing these antibodies for a few different projects. Does anyone on here use these two antibodies for IHC in FFPE tissues? I'm going to use them in bright field, but we may also turn to IF for other studies. I'd be grateful for any information from what clone/company do you buy from and retrieval methods to primary antibody incubations/dilutions. Thank you all for any information, Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartwood@cshs.org From wsimons <@t> athensgastro.com Wed Dec 3 12:05:55 2014 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Wed Dec 3 12:06:01 2014 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUkU6IFByb3N0YXRlIE5lZWRsZSBCeA==?= In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25C006C0264@MERCERMAIL.MercerU.local> References: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> <9BF995BC0E47744E9673A41486E24EE25C006C0264@MERCERMAIL.MercerU.local> Message-ID: <20141203180556.27189.qmail@server276.com> I concur with Shirley~ this will be my next order for my GI lab. Wanda > -------Original Message------- > From: Shirley A. Powell > To: Laurie Colbert , Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] RE: Prostate Needle Bx > Sent: Dec 03 '14 12:00 > > I have discovered a wonderful cassette from Cancer Diagnostics, it is called the Autocassette and has an oval concave cavity with no corners for small pieces of tissue to get caught in and the solutions pass through it very nicely.??I use it for small research samples, like shrimp gills similar in size to the prostate biopsies, and I lose nothing.??I use the yellow ones, VB1005 but they have other colors and also some with smaller grid.??Highly recommend them.?? > > Shirley > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Wednesday, December 03, 2014 11:49 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Prostate Needle Bx > > How do others process prostate needle bx's - what kind of cassettes do you use???Do you use sponges???Do you wrap the tissue in lens paper???Do you process them on a biopsy (short) run???Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. > > Laurie Colbert > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From carl.hobbs <@t> kcl.ac.uk Wed Dec 3 12:05:16 2014 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Dec 3 12:10:48 2014 Subject: [Histonet] FoxP3 and Geminin Immunohistochemistry Message-ID: <1417629913937.37454@kcl.ac.uk> ?I have no xp re Geminin but, I have posted images of FoxP3 in FFPWS on the Histonet Images website. The two abs work well for me. I used a homemade std Citric acid HIER solution. Looks like you have choices, given Katy Milne's response! Good luck. carl From nto <@t> stowers.org Wed Dec 3 13:00:53 2014 From: nto <@t> stowers.org (Thomas, Nancy) Date: Wed Dec 3 13:01:14 2014 Subject: [Histonet] RE: MMA Mounting Issue with Haupts Adhesive In-Reply-To: References: Message-ID: Jesse, I have discovered that there are many different ways to work with MMA. Probably no two techs are doing it exactly the same because there are so many steps in which to optimize it for your lab. From reading your protocol, I see similarities and differences. Rather than saran wrap, I use a thicker plastic that I cut into strips ( the size of the slide) from the plastic bags that we use for discarding paraffin waste. Although this is different from your protocol, I still think that what I do to remove the plastic might work for you: just before removing the plastic strip, I shoot it with a blast of freeze spray. The plastic just about falls off. Nancy Thomas Stowers Institute for Medical Research Kansas City, MO -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hernandez, Jesus Willibaldo Sent: Wednesday, December 03, 2014 11:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MMA Mounting Issue with Haupts Adhesive Hi all, I had a question about how to properly mount MMA sectioned slides using Haupt's (Dorn and Hart Microedge). My protocol is as follows: 1. Stick Glass Slide into 50mL Falcon Tube with Haupts solution (1:1 50% EtOH:50% Haupts). 2. Rotate the tube to ensure entire coating of glass slide. 3. Let glass slide dry in fume hood for about 10minutes. 4. Remove cut microtome sections (range: 50-5microns). 5. Place cut sections in 70%EtOH to soften the plastic and unroll. (sections are coming out rolled up like scrolls) 6. Remove a section from the 70%EtOH using forceps and try unrolling it with a needle on a glass slide. 7. Once the section(s) is stretched on the coated glass slide, cover with saran wrap. 8. Cover the wrapped slide with coarse filter paper and use a small seam roller to roll the sections on the glass slide as flat as possible. This will also help remove excess 70%EtOH. 9. Remove the filter paper and stack glass slides in a slide press or similar device. Place tongue depressors or small pieces of wood equivalent to the slide length that are about 1cm thick at the end of the glass slide stack. 10. Let slides dry over night at room temperature in press. 11. Remove the slides from the press and unwrap the saran wrap to expose your adhered sample. Questions/Comments: 1. When I pull off the saran wrap, the sample sometimes comes off. The plastic in the sample turns white and dries out and does not attach. This is seen in the majority of my tissue sections. 2. The only time I have noticed consistent attachment is for a 5micron sized section. 3. How can I adjust the microtome so that the samples do not come off so scroll-like? My cutting angle is typically either 0 or 2.5 degrees. I also apply 70% EtOH to the block surface to soften the plastic. Overall goal: Get the tissue sections adhered to the glass slides so that I can proceed with staining. Thank you all. Regards, Jesse Hernandez _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tejohnson <@t> genoptix.com Wed Dec 3 13:03:58 2014 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Wed Dec 3 13:04:06 2014 Subject: [Histonet] re: Flotation bath temperatures Message-ID: Dear all, I am changing the language to flotation bath (instead of water bath, since that is entirely different lab equipment). I understand there is no longer a CAP requirement to record temperatures. Is there a GLP (or GxP) requirement to do so? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From liz <@t> premierlab.com Wed Dec 3 13:19:09 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Dec 3 13:19:18 2014 Subject: [Histonet] RE: re: Flotation bath temperatures In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDD5E@SBS2K8.premierlab.local> Teri We do, it's more of a combination use log for the microtome and tissue flotation bath, we record temperature and that we cleaned the unit, used fresh water and also cleaned the microtome. This document also records the yearly calibration of the floatation bath and the yearly PM of the microtome. I do need to add that both the calibration and PM will have additional documentation that is stored in a designated binder. I can send you a copy of the form in a separate e-mail if you would like it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Wednesday, December 03, 2014 12:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] re: Flotation bath temperatures Dear all, I am changing the language to flotation bath (instead of water bath, since that is entirely different lab equipment). I understand there is no longer a CAP requirement to record temperatures. Is there a GLP (or GxP) requirement to do so? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne <@t> rwjuh.edu Wed Dec 3 13:23:02 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Wed Dec 3 13:23:08 2014 Subject: [Histonet] RE: re: Flotation bath temperatures In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECDD5E@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECDD5E@SBS2K8.premierlab.local> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24EDFBC8@vap1014.win.rwjuh.edu> Although it is not in the Anatomic CAP checklist, there is a new All Common question that does address this, and which, if I'm understanding it, would include flotation baths. All Common checklist, COM.30750, Phase II "Temperatures are checked and recorded each day of use for all temperature-dependent equipment and environments using a calibrated thermometer." Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, December 03, 2014 2:19 PM To: Teri Johnson; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: re: Flotation bath temperatures Teri We do, it's more of a combination use log for the microtome and tissue flotation bath, we record temperature and that we cleaned the unit, used fresh water and also cleaned the microtome. This document also records the yearly calibration of the floatation bath and the yearly PM of the microtome. I do need to add that both the calibration and PM will have additional documentation that is stored in a designated binder. I can send you a copy of the form in a separate e-mail if you would like it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Wednesday, December 03, 2014 12:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] re: Flotation bath temperatures Dear all, I am changing the language to flotation bath (instead of water bath, since that is entirely different lab equipment). I understand there is no longer a CAP requirement to record temperatures. Is there a GLP (or GxP) requirement to do so? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Albert.Santiago <@t> uphs.upenn.edu Wed Dec 3 13:24:59 2014 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Wed Dec 3 13:25:05 2014 Subject: [Histonet] H&E stainer validation Message-ID: Hello fellow histonetters, Does anyone have a procedure for validation of a new H&E stainer, if you do, can you please share it with me? Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago@uphs.upenn.edu From suetp918 <@t> comcast.net Wed Dec 3 13:28:26 2014 From: suetp918 <@t> comcast.net (Sue) Date: Wed Dec 3 13:28:36 2014 Subject: [Histonet] RE: Prostate Needle Bx In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25C006C0264@MERCERMAIL.MercerU.local> References: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> <9BF995BC0E47744E9673A41486E24EE25C006C0264@MERCERMAIL.MercerU.local> Message-ID: <1859415008.3168460.1417634906958.JavaMail.zimbra@comcast.net> I have seen these cassettes and while they are nice they are rather expensive and with re-embursemnt down everyone is looking at their budget.? Also due to the design and the use of bar codes they do not always fit in the engravers.? I still like the idea but with the amount of prostates we do my budget will be hit.? I also have no more areas on my engraver to put yet another cassette. ? STP TJUH From Albert.Santiago <@t> uphs.upenn.edu Wed Dec 3 13:29:04 2014 From: Albert.Santiago <@t> uphs.upenn.edu (Santiago, Albert) Date: Wed Dec 3 13:29:26 2014 Subject: [Histonet] New H&E stainer validation Message-ID: Hello fellow histonetters, Does anyone have a procedure for validation of a new H&E stainer, if you do, can you please share it with me? Thank you From aobrien88 <@t> comcast.net Wed Dec 3 13:52:48 2014 From: aobrien88 <@t> comcast.net (aobrien88@comcast.net) Date: Wed Dec 3 13:53:10 2014 Subject: [Histonet] RE: Prostate Needle Bx In-Reply-To: <1859415008.3168460.1417634906958.JavaMail.zimbra@comcast.net> References: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> <9BF995BC0E47744E9673A41486E24EE25C006C0264@MERCERMAIL.MercerU.local> <1859415008.3168460.1417634906958.JavaMail.zimbra@comcast.net> Message-ID: <788689527.8572877.1417636368956.JavaMail.zimbra@comcast.net> We are a prostate lab and use regular hinged cassettes with sponges. We ink each case with a different color ink, using 5 colors rotating in order. We microwave process with a program that is around 55 minutes. The program is 100% alcohol, isopropyl and paraffin. We have had great success with it. Hope that helps. Andrea ----- Original Message ----- From: "Sue" To: "Shirley A. Powell" Cc: "histonet" Sent: Wednesday, December 3, 2014 1:28:26 PM Subject: Re: [Histonet] RE: Prostate Needle Bx I have seen these cassettes and while they are nice they are rather expensive and with re-embursemnt down everyone is looking at their budget. Also due to the design and the use of bar codes they do not always fit in the engravers. I still like the idea but with the amount of prostates we do my budget will be hit. I also have no more areas on my engraver to put yet another cassette. STP TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Wed Dec 3 14:23:55 2014 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Dec 3 14:24:04 2014 Subject: [Histonet] RE: Prostate Needle Bx In-Reply-To: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> Message-ID: <761E2B5697F795489C8710BCC72141FF367B9AC6@ex07.net.ucsf.edu> Currently we use the sponges but have always had problems with getting the cores to be straight and getting a whole core in one tissue section. Plus counting the cores and correlating that at embedding. However, we are testing out a system called SmartBx that is an entire system starting at taking the Bx. It uses an imaging system to guide the bx, then a special bx needle system that places the core in a special cassette on a piece of treated paper - perfectly straight and flat. The cores adhere to the paper and the paper goes through to the final block using special molds at embedding. At grossing the bx/paper is simply placed in a cassette and sent to processing. At embedding the paper w/bx is placed tissue-down in the special mold and pressed down with an associated tamper. The final block has perfectly straight cores at the same level exactly 100um from the block face. There has been a learning curve but overall it makes prostate bx's very easy to handle in grossing and at embedding. the slides have full face cores on every single one. Kind of expensive though, so probably only worthwhile if you do a lot of these. http://www.uc-care.com/products/smartbx Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, December 03, 2014 8:49 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Prostate Needle Bx How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aobrien88 <@t> comcast.net Wed Dec 3 14:36:30 2014 From: aobrien88 <@t> comcast.net (aobrien88@comcast.net) Date: Wed Dec 3 14:36:57 2014 Subject: [Histonet] RE: Prostate Needle Bx In-Reply-To: <20141203180556.27189.qmail@server276.com> References: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> <9BF995BC0E47744E9673A41486E24EE25C006C0264@MERCERMAIL.MercerU.local> <20141203180556.27189.qmail@server276.com> Message-ID: <1699366919.8618670.1417638990318.JavaMail.zimbra@comcast.net> My Medical Director published a paper on our procedure. If you would be interested I can ask him to share it with you. Andrea ----- Original Message ----- From: wsimons@athensgastro.com To: "Shirley A. Powell" , "Laurie Colbert" , "histonet" Sent: Wednesday, December 3, 2014 12:05:55 PM Subject: Re: [Histonet] RE: Prostate Needle Bx I concur with Shirley~ this will be my next order for my GI lab. Wanda > -------Original Message------- > From: Shirley A. Powell > To: Laurie Colbert , Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] RE: Prostate Needle Bx > Sent: Dec 03 '14 12:00 > > I have discovered a wonderful cassette from Cancer Diagnostics, it is called the Autocassette and has an oval concave cavity with no corners for small pieces of tissue to get caught in and the solutions pass through it very nicely. I use it for small research samples, like shrimp gills similar in size to the prostate biopsies, and I lose nothing. I use the yellow ones, VB1005 but they have other colors and also some with smaller grid. Highly recommend them. > > Shirley > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Wednesday, December 03, 2014 11:49 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Prostate Needle Bx > > How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. > > Laurie Colbert > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Wed Dec 3 19:07:26 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Wed Dec 3 19:07:29 2014 Subject: [Histonet] Re: Prostate Needle Bx Message-ID: Laurie Colbert asks about processing prostate needle biopsy specimens. The subject is extensively reviewed in this month's or last month's Archives of Pathology. Your pathologists have copies of this if they're fellows of the College of American Pathologists. Here's the method as presented in that article - I've been doing this for a good many years now. The urologist should submit six containers, three for each side of the prostate, each container containing two or more cores. No more than two cores should be put in one cassette. Use those little blue sponges in the cassettes to keep the cores flat. Measure each core, and record the measurements. Don't dye the cores with eosin (fluorescent) - if you want to dye them use safranin O. Process with other small biopsy specimens. Embed using a tamper to get the cores flat in the bottom of the boat. Cut sections onto slides suitable for immunohistochemistry. Cut 5 sections of each block, with little if any trimming. Stain slides 1, 3, and 5 with H & E. Reserve slides 2 & 4 for possible immunohistochemistry (needed in about a tenth of cases). It's a lot of work, but it makes cancer diagnoses. Expect a cancer diagnosis in about a third of cases. Bob Richmond Samurai Pathologist Maryville TN From j.rowaihi <@t> alborglaboratories.com Thu Dec 4 03:29:36 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Thu Dec 4 03:29:41 2014 Subject: [Histonet] Non-GYN Prep Safety In-Reply-To: References: Message-ID: <03c501d00fa4$d37d1eb0$7a775c10$@rowaihi@alborglaboratories.com> We process all conventional cytology specimens inside safety cabinet even the centrifuge. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amanda Reichard Sent: Wednesday, December 03, 2014 7:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Non-GYN Prep Safety Question for you all Do you process Non-GYN specimens under a hood? What type of precautions are taken for suspect mycobacterium specimens? Are your ThinPrep machines under a hood as well? Thanks in advance Amanda Reichard, HTL (ASCP)cm Histotechnologist Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Thu Dec 4 07:34:05 2014 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Dec 4 07:34:09 2014 Subject: [Histonet] RE: Prostate Needle Bx--Inking core biopsies for patient identification In-Reply-To: <761E2B5697F795489C8710BCC72141FF367B9AC6@ex07.net.ucsf.edu> References: <12ECD7346266D74691EC2BFC75285E453814AC3A@BFL323E10.pathmdlabs.local> <761E2B5697F795489C8710BCC72141FF367B9AC6@ex07.net.ucsf.edu> Message-ID: <0A05EC63892F834395DCC6047CAD0C5B18B82B@UPHQMSX01.uropartners.local> Our histology supervisor, Andrea O'Brien, asked me to post the reference for our prostate biopsy inking technique. Andrea did a lot of the development work and is one of the co-authors. The reference is: Raff LJ, et al: The Effectiveness of Inking Needle Core Prostate Biopsies for Preventing Patient Specimen Identification Errors: A Technique to Address Joint Commission Patient Safety Goals in specialty Laboratories. Arch Pathol Lab Med. 2009;133:295-297 We have made some minor changes since original publication, please feel free to email for additional information. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 lraff@uropartners.com From snholmes64 <@t> gmail.com Thu Dec 4 10:56:46 2014 From: snholmes64 <@t> gmail.com (Snholmes64@gmail.com) Date: Thu Dec 4 10:56:54 2014 Subject: [Histonet] TBS Microtome Message-ID: <4864709B-17CE-44AB-B705-B50BB949A4F8@gmail.com> Need to talk to someone that has experience with TBS microtones. Especially when working with animal tissue. Could you pm me privately at snholmes64@gmail.com Thank you! Sent from my iPhone From Nancy_Schmitt <@t> pa-ucl.com Thu Dec 4 12:19:23 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Dec 4 12:19:30 2014 Subject: [Histonet] Prostate Needle Bx In-Reply-To: <20141204180353.894BD2D00B4@mail.pa-ucl.com> References: <20141204180353.894BD2D00B4@mail.pa-ucl.com> Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C36011593FB72@PEITHA.wad.pa-ucl.com> Our group puts the biopsies between sponges - they don't move around or get tangled and we process them with the small biopsies. Good results. Nancy Schmitt United Clinical laboratories Dubuque, IA **************************************** > Sent: Wednesday, December 03, 2014 11:49 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Prostate Needle Bx > > How do others process prostate needle bx's - what kind of cassettes > do you use?????Do you use sponges?????Do you wrap the tissue in lens paper?????Do you process them on a biopsy (short) run?????Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. > > Laurie Colbert NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From jqb7 <@t> cdc.gov Thu Dec 4 12:22:20 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu Dec 4 12:22:38 2014 Subject: [Histonet] RE: Prostate Needle Bx In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C36011593FB72@PEITHA.wad.pa-ucl.com> References: <20141204180353.894BD2D00B4@mail.pa-ucl.com> <906B4DA90ED1DB4DB6C7E94D7CEE6C36011593FB72@PEITHA.wad.pa-ucl.com> Message-ID: <3B2CD438E1628A41BD687E98B963B78120895BC0@EMBX-CLFT4.cdc.gov> But you can't use sponges with the microwave processors............................at least not with the Sakura Xpress Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, December 04, 2014 1:19 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Prostate Needle Bx Our group puts the biopsies between sponges - they don't move around or get tangled and we process them with the small biopsies. Good results. Nancy Schmitt United Clinical laboratories Dubuque, IA **************************************** > Sent: Wednesday, December 03, 2014 11:49 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Prostate Needle Bx > > How do others process prostate needle bx's - what kind of cassettes > do you use?????Do you use sponges?????Do you wrap the tissue in lens paper?????Do you process them on a biopsy (short) run?????Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. > > Laurie Colbert NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aobrien88 <@t> comcast.net Thu Dec 4 12:43:35 2014 From: aobrien88 <@t> comcast.net (aobrien88@comcast.net) Date: Thu Dec 4 12:43:52 2014 Subject: [Histonet] RE: Prostate Needle Bx In-Reply-To: <3B2CD438E1628A41BD687E98B963B78120895BC0@EMBX-CLFT4.cdc.gov> References: <20141204180353.894BD2D00B4@mail.pa-ucl.com> <906B4DA90ED1DB4DB6C7E94D7CEE6C36011593FB72@PEITHA.wad.pa-ucl.com> <3B2CD438E1628A41BD687E98B963B78120895BC0@EMBX-CLFT4.cdc.gov> Message-ID: <239025527.9358257.1417718615023.JavaMail.zimbra@comcast.net> We have a Milestone Histos 3 Microwave processor, and we process our prostate bx's and bladder bx's between 2 sponges. The sponges are moist with 10% formalin to keep the tissue from drying out. The bx's don't move around or get tangled. It works very well. Andrea O'Brien HT(ASCP) ----- Original Message ----- From: "Jeanine Sanders (CDC/OID/NCEZID)" To: "Nancy Schmitt" , "histonet" Sent: Thursday, December 4, 2014 12:22:20 PM Subject: [Histonet] RE: Prostate Needle Bx But you can't use sponges with the microwave processors............................at least not with the Sakura Xpress Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, December 04, 2014 1:19 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Prostate Needle Bx Our group puts the biopsies between sponges - they don't move around or get tangled and we process them with the small biopsies. Good results. Nancy Schmitt United Clinical laboratories Dubuque, IA **************************************** > Sent: Wednesday, December 03, 2014 11:49 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Prostate Needle Bx > > How do others process prostate needle bx's - what kind of cassettes > do you use?????Do you use sponges?????Do you wrap the tissue in lens paper?????Do you process them on a biopsy (short) run?????Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. > > Laurie Colbert NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wsimons <@t> athensgastro.com Thu Dec 4 13:21:55 2014 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Thu Dec 4 13:22:00 2014 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gUkU6IFByb3N0YXRlIE5lZWRsZSBCeA==?= In-Reply-To: <239025527.9358257.1417718615023.JavaMail.zimbra@comcast.net> References: <20141204180353.894BD2D00B4@mail.pa-ucl.com> <906B4DA90ED1DB4DB6C7E94D7CEE6C36011593FB72@PEITHA.wad.pa-ucl.com> <3B2CD438E1628A41BD687E98B963B78120895BC0@EMBX-CLFT4.cdc.gov> <239025527.9358257.1417718615023.JavaMail.zimbra@comcast.net> Message-ID: <20141204192155.15050.qmail@server276.com> I had issues with the warranty on one of my processors because of sponges. It would be interesting to hear from Milestone and other vendors about this issue. I discontinued the use of sponges decades ago~ Wanda K Simons, HT (ASCP) Athens Gastroenterology Association 3320 Old Jefferson Road Athens, Ga 30607 > -------Original Message------- > From: aobrien88@comcast.net > To: Jeanine Sanders (CDC/OID/NCEZID) > Cc: histonet , Nancy Schmitt > Subject: Re: [Histonet] RE: Prostate Needle Bx > Sent: Dec 04 '14 13:46 > > We have a Milestone Histos 3 Microwave processor, and we process our prostate bx's and bladder bx's between 2 sponges. The sponges are moist with 10% formalin to keep the tissue from drying out. The bx's don't move around or get tangled. It works very well. > > Andrea O'Brien HT(ASCP) > > ----- Original Message ----- > > From: "Jeanine Sanders (CDC/OID/NCEZID)" > To: "Nancy Schmitt" , "histonet" > Sent: Thursday, December 4, 2014 12:22:20 PM > Subject: [Histonet] RE: Prostate Needle Bx > > But you can't use sponges with the microwave processors............................at least not with the Sakura Xpress > > > Jeanine H. Sanders > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 404-639-3590 > jqb7@cdc.gov > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt > Sent: Thursday, December 04, 2014 1:19 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Prostate Needle Bx > > Our group puts the biopsies between sponges - they don't move around or get tangled and we process them with the small biopsies. Good results. > > Nancy Schmitt > United Clinical laboratories > Dubuque, IA > **************************************** > > > Sent: Wednesday, December 03, 2014 11:49 AM > > To: Histonet Post (histonet@lists.utsouthwestern.edu) > > Subject: [Histonet] Prostate Needle Bx > > > > How do others process prostate needle bx's - what kind of cassettes > > do you use?????Do you use sponges?????Do you > wrap the tissue in lens paper?????Do you process them on a biopsy (short) run?????Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. > > > > Laurie Colbert > > > > NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren <@t> gmail.com Thu Dec 4 15:11:42 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Dec 4 15:11:48 2014 Subject: [Histonet] Re: Prostate Needle Bx In-Reply-To: References: Message-ID: I would like to amplify Dr. Richmond's comments by saying that the grossing and embedding are absolutely critical with prostate bxs. While on assignment in the north, I helped to improve a protocol in use at a regional medical center. Changing cassettes or purchasing esoteric equipment was out of the question. I found the most important thing, from the pathologist's standpoint, was getting complete, full, representative sections, right from the first level to the last. They can't diagnose what isn't on the slide, or heaven forfend, tissue that has been cut away trying to get a full face section. The cutters can't get a full section unless the embedding is *ABSOLUTELY FLAT*. This takes skill and time. Two sponges should be used to keep the bxs flat in processing, but the bxs will pretty much come out of wax the way they went in, with a little shrinkage. So *you cannot have the bx folded over, or overlapping another bx when it goes onto the first sponge.* I find a good technique for the grosser is to use a cut off disposable pipette to suck up the bx and place it onto the sponge. This avoids crush artifact from forceps as well . To reiterate; Gross it flat and straight (between sponges), embed it flat and straight, and the cutter has the best chance of getting full sections. Dyeing the bxs with safranin helps the cutter to visualize when they have a full face section. Sincerely. Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Dec 3, 2014 at 7:07 PM, Bob Richmond wrote: > Laurie Colbert asks about processing prostate needle biopsy specimens. > > The subject is extensively reviewed in this month's or last month's > Archives of Pathology. Your pathologists have copies of this if they're > fellows of the College of American Pathologists. Here's the method as > presented in that article - I've been doing this for a good many years now. > > The urologist should submit six containers, three for each side of the > prostate, each container containing two or more cores. No more than two > cores should be put in one cassette. Use those little blue sponges in the > cassettes to keep the cores flat. Measure each core, and record the > measurements. Don't dye the cores with eosin (fluorescent) - if you want to > dye them use safranin O. > > Process with other small biopsy specimens. > > Embed using a tamper to get the cores flat in the bottom of the boat. > > Cut sections onto slides suitable for immunohistochemistry. Cut 5 sections > of each block, with little if any trimming. Stain slides 1, 3, and 5 with H > & E. Reserve slides 2 & 4 for possible immunohistochemistry (needed in > about a tenth of cases). > > It's a lot of work, but it makes cancer diagnoses. Expect a cancer > diagnosis in about a third of cases. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Thu Dec 4 19:10:45 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Dec 4 19:10:50 2014 Subject: [Histonet] Re: Prostate Needle Bx In-Reply-To: References: Message-ID: Jay Lundgren thought of several more things I didn't think of. It's essential to get the biopsy cores strung out straight - remember the pathologist has to measure them on the slide. You do of course have to use two of the blue sponges. If the tissue is adequately fixed, you shouldn't get crush artifact handling the specimens with tweezers, which is what I do (and I think what the large volume labs do). I haven't used safranin O for prostate biopsies, though I certainly have for GI biopsies. Your microbiology lab uses quite a lot of safranin O solution for the Gram stain, and this preparation works quite well for small biopsy specimens. (Once again, don't use eosin - it fluoresces and interferes with fluorescent techniques.) Bob Richmond Samurai Pathologist Maryville TN On Thu, Dec 4, 2014 at 4:11 PM, Jay Lundgren wrote: > I would like to amplify Dr. Richmond's comments by saying that the > grossing and embedding are absolutely critical with prostate bxs. > > While on assignment in the north, I helped to improve a protocol in > use at a regional medical center. Changing cassettes or purchasing > esoteric equipment was out of the question. I found the most important > thing, from the pathologist's standpoint, was getting complete, full, > representative sections, right from the first level to the last. They > can't diagnose what isn't on the slide, or heaven forfend, tissue that has > been cut away trying to get a full face section. > > The cutters can't get a full section unless the embedding is *ABSOLUTELY > FLAT*. This takes skill and time. > > Two sponges should be used to keep the bxs flat in processing, but > the bxs will pretty much come out of wax the way they went in, with a > little shrinkage. So *you cannot have the bx folded over, or overlapping > another bx when it goes onto the first sponge.* > > I find a good technique for the grosser is to use a cut off > disposable pipette to suck up the bx and place it onto the sponge. This > avoids crush artifact from forceps as well > . > To reiterate; Gross it flat and straight (between sponges), embed it > flat and straight, and the cutter has the best chance of getting full > sections. Dyeing the bxs with safranin helps the cutter to visualize when > they have a full face section. > > Sincerely. > Jay A. Lundgren, M.S., HTL (ASCP) > > > > On Wed, Dec 3, 2014 at 7:07 PM, Bob Richmond wrote: > >> Laurie Colbert asks about processing prostate needle biopsy specimens. >> >> The subject is extensively reviewed in this month's or last month's >> Archives of Pathology. Your pathologists have copies of this if they're >> fellows of the College of American Pathologists. Here's the method as >> presented in that article - I've been doing this for a good many years >> now. >> >> The urologist should submit six containers, three for each side of the >> prostate, each container containing two or more cores. No more than two >> cores should be put in one cassette. Use those little blue sponges in the >> cassettes to keep the cores flat. Measure each core, and record the >> measurements. Don't dye the cores with eosin (fluorescent) - if you want >> to >> dye them use safranin O. >> >> Process with other small biopsy specimens. >> >> Embed using a tamper to get the cores flat in the bottom of the boat. >> >> Cut sections onto slides suitable for immunohistochemistry. Cut 5 sections >> of each block, with little if any trimming. Stain slides 1, 3, and 5 with >> H >> & E. Reserve slides 2 & 4 for possible immunohistochemistry (needed in >> about a tenth of cases). >> >> It's a lot of work, but it makes cancer diagnoses. Expect a cancer >> diagnosis in about a third of cases. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From jqb7 <@t> cdc.gov Fri Dec 5 05:06:12 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Fri Dec 5 05:07:12 2014 Subject: [Histonet] RE: Prostate Needle Bx In-Reply-To: <20141204192155.15050.qmail@server276.com> References: <20141204180353.894BD2D00B4@mail.pa-ucl.com> <906B4DA90ED1DB4DB6C7E94D7CEE6C36011593FB72@PEITHA.wad.pa-ucl.com> <3B2CD438E1628A41BD687E98B963B78120895BC0@EMBX-CLFT4.cdc.gov> <239025527.9358257.1417718615023.JavaMail.zimbra@comcast.net> <20141204192155.15050.qmail@server276.com> Message-ID: <3B2CD438E1628A41BD687E98B963B78120895E51@EMBX-CLFT4.cdc.gov> I just received an update from Sakura and the issue seems to primarily be a carry-over issue. -----Original Message----- From: wsimons@athensgastro.com [mailto:wsimons@athensgastro.com] Sent: Thursday, December 04, 2014 2:22 PM To: aobrien88@comcast.net; Sanders, Jeanine (CDC/OID/NCEZID) Cc: histonet; Nancy Schmitt Subject: Re: [Histonet] RE: Prostate Needle Bx I had issues with the warranty on one of my processors because of sponges. It would be interesting to hear from Milestone and other vendors about this issue. I discontinued the use of sponges decades ago~ Wanda K Simons, HT (ASCP) Athens Gastroenterology Association 3320 Old Jefferson Road Athens, Ga 30607 > -------Original Message------- > From: aobrien88@comcast.net > To: Jeanine Sanders (CDC/OID/NCEZID) > Cc: histonet , Nancy Schmitt > > Subject: Re: [Histonet] RE: Prostate Needle Bx > Sent: Dec 04 '14 13:46 > > We have a Milestone Histos 3 Microwave processor, and we process our > prostate bx's and bladder bx's between 2 sponges. The sponges are moist with 10% formalin to keep the tissue from drying out. The bx's don't move around or get tangled. It works very well. > > Andrea O'Brien HT(ASCP) > > ----- Original Message ----- > > From: "Jeanine Sanders (CDC/OID/NCEZID)" > To: "Nancy Schmitt" , "histonet" > > Sent: Thursday, December 4, 2014 12:22:20 PM > Subject: [Histonet] RE: Prostate Needle Bx > > But you can't use sponges with the microwave > processors............................at least not with the Sakura > Xpress > > > Jeanine H. Sanders > Centers for Disease Control and Prevention Infectious Diseases > Pathology Branch > 404-639-3590 > jqb7@cdc.gov > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt > Sent: Thursday, December 04, 2014 1:19 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Prostate Needle Bx > > Our group puts the biopsies between sponges - they don't move around > or get tangled and we process them with the small biopsies. Good results. > > Nancy Schmitt > United Clinical laboratories > Dubuque, IA > **************************************** > > > Sent: Wednesday, December 03, 2014 11:49 AM > To: Histonet Post > (histonet@lists.utsouthwestern.edu) > > Subject: [Histonet] Prostate Needle Bx > > How do others process > prostate needle bx's - what kind of cassettes > do you use?????Do you > use sponges?????Do you wrap the tissue in lens paper?????Do you > process them on a biopsy (short) run?????Our bx's always seem to be > less than optimal, but we do not have problems with other small bx's. > > > > Laurie Colbert > > > > NOTICE: This email may contain legally privileged information. The > information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rmweber113 <@t> comcast.net Fri Dec 5 07:43:15 2014 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Fri Dec 5 07:43:47 2014 Subject: [Histonet] Billing codes 2015 In-Reply-To: <389170114.10766209.1417786853795.JavaMail.zimbra@comcast.net> Message-ID: <712475632.10767980.1417786995288.JavaMail.zimbra@comcast.net> Hi,? I was wondering if anyone has heard of any new billing codes for 2015 in regards to cytology,?urology pathology or immunohistochemistry. ?If so, could you please share? ? Thanks so much for your help. ? Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 From bcdukes <@t> lexhealth.org Fri Dec 5 12:06:29 2014 From: bcdukes <@t> lexhealth.org (Blake Taylor) Date: Fri Dec 5 12:06:36 2014 Subject: [Histonet] Block and Slide Retention In-Reply-To: <201412051801.sB5I19Td015250@rly08h.srv.mailcontrol.com> References: <201412051801.sB5I19Td015250@rly08h.srv.mailcontrol.com> Message-ID: We currently keep our blocks and slides indefinitely but would like to revise our retention policy. I know CAP has a minimum of 10 years, Just wanted some feedback on what others thoughts on this are. We are thinking maybe 15-18years??? Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From Evelyne.Kokoskin <@t> oahpp.ca Fri Dec 5 12:30:11 2014 From: Evelyne.Kokoskin <@t> oahpp.ca (Evelyne Kokoskin) Date: Fri Dec 5 12:30:19 2014 Subject: [Histonet] Blood smear question Message-ID: <2A63092EB60FF14D91637BA029A56E361DBF6281@OTO101PEX03V.oahpp.ca> We are looking at infectious agents in thin blood smears and how to eliminate them before staining using fixation. Other than methanol, what are other fixatives that could possibly be used for fixing thin films ( that would destroy infectious agents) and allow staining with Giemsa stain. Thanks, Evelyne From taylor <@t> prometheushealthcare.com Fri Dec 5 12:46:38 2014 From: taylor <@t> prometheushealthcare.com (Taylor Rinaldi) Date: Fri Dec 5 12:43:12 2014 Subject: [Histonet] Histology Openings in California Message-ID: <041b01d010bb$cde833a0$69b89ae0$@prometheushealthcare.com> A growing anatomic pathology laboratory in Modesto, CA is looking to hire in their Histology department. Bench and managerial positions available. ASCP certification required. These are permanent, full time opportunities offering relocation assistance. Please reach out to me for immediate consideration. Thanks so much! Taylor Rinaldi Recruiter Prometheus Healthcare Office (301) 693-9057 Taylor@prometheushealthcare.com From taylor <@t> prometheushealthcare.com Fri Dec 5 12:56:25 2014 From: taylor <@t> prometheushealthcare.com (Taylor Rinaldi) Date: Fri Dec 5 12:52:58 2014 Subject: [Histonet] Histology opening in Boulder, Colorado Message-ID: <042801d010bd$2bd11350$837339f0$@prometheushealthcare.com> A growing research laboratory in Boulder, Co is looking to hire in their Histology department. They are looking a Histotechnician with at least 1-2 years' experience. ASCP certification preferred, not required. This is a permanent, full time opportunity. Please reach out to me for immediate consideration. Thanks so much! Taylor Rinaldi Recruiter Prometheus Healthcare Office (301) 693-9057 Taylor@prometheushealthcare.com From rjbuesa <@t> yahoo.com Fri Dec 5 13:01:03 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 5 13:01:09 2014 Subject: [Histonet] Blood smear question In-Reply-To: <2A63092EB60FF14D91637BA029A56E361DBF6281@OTO101PEX03V.oahpp.ca> References: <2A63092EB60FF14D91637BA029A56E361DBF6281@OTO101PEX03V.oahpp.ca> Message-ID: <1322250955.1563415.1417806064024.JavaMail.yahoo@jws10096.mail.ne1.yahoo.com> Now you got me confused! If you are looking for infectious agents, why would you like to destroy them?????Can you elaborate? Ren? J.? On Friday, December 5, 2014 1:30 PM, Evelyne Kokoskin wrote: We are looking at infectious agents in thin blood smears and how to eliminate them before staining using fixation. Other than methanol, what are other? fixatives that could possibly be used for? fixing thin films ( that would destroy infectious agents) and allow? staining with Giemsa stain. Thanks, Evelyne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Fri Dec 5 13:15:00 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Fri Dec 5 13:15:23 2014 Subject: [Histonet] Happy Holidays from RELIA Solutions 12-5-2014 Exciting New Opportunities to Share!!! Message-ID: <15559281.1417806900944.JavaMail.root@wamui-hunyo.atl.sa.earthlink.net> Hello Histonetters!! Happy Holidays!! While a job change is the last thing on most people?s minds this time of year; May I take a minute of your time to ask; Is a job change the next logical step in your career? This is a special bulletin for you. Some of my best clients are expanding and as a result some fantastic opportunities are open right now. I wanted to touch base and give you a heads up. Your next opportunity might be just around the corner and I might have it for you. If you are looking for a position right now please contact me RIGHT AWAY!! In addition to these opportunities I have new positions coming in DAILY!! If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out that would be great too. To do that, just shoot me an email at relia1@earthlink.net or call me toll free at 866-607-3542. Here is a list of my current opportunities: HISTOLOGY SUPERVISORS/MANAGERS: Histology Supervisor - Modesto, CA Histology Manager ? Flagstaff, AZ AP Manager ? Hammond, IN Lead Histology Tech ? St. Louis, MO Asst. Supervisor ? Las Cruces, NM Lead Histotech ? Yuma, AZ Histology Supervisor ? Atlanta, GA HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Histology Tech ? Yuma, AZ Histotechnician ? Hammond, IN Histotech DAYS ? Modesto, CA Histotech NIGHTS - Modesto, CA Histotechnologist ? Atlanta, GA Junior Histotech ? Boulder, CO Grossing Tech ? Long Island, NY Junior Histotech ? Boston, MA My clients offer competitive salaries, great benefits and in most cases relocation assistance and or sign on bonuses. **While all of these clients would LOVE to hire right away; they are also willing to start the process with a phone interview now and continue the process after the holidays if that would be better for you! Remember, I offer a 500.00 dollar referral fee if I place someone you refer to me. I really appreciate you taking time out of your busy day to read my e-mail Thanks Again! Thanks-Pam Thank you! Pam M. Barker President RELIA 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: 407-657-2027 Fax: 407-678-2788 Cell: 407-353-5070 e-mail: relia1@earthlink.net Toll Free: 866-60-RELIA (866-607-3542) From rsrichmond <@t> gmail.com Sat Dec 6 16:46:11 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Dec 6 16:46:14 2014 Subject: [Histonet] Re: Block and Slide Retention Message-ID: Blake Taylor, Surgical Pathology Supervisor, Lexington Medical Center in SC asks: >>We currently keep our blocks and slides indefinitely but would like to revise our retention policy. I know CAP has a minimum of 10 years, Just wanted some feedback on what others thoughts on this are. We are thinking maybe 15-18 years???<< I think that with the rapid advent of molecular and genetic studies that can be done on paraffin embedded tissue, that the 10 year minimum is going to need to be extended, though I haven't heard of any proposals. A non-trivial problem, because of the temperature control needed for storing paraffin blocks. A related problem: what should be done with archived blocks when a lab closes, as so many will in the next few years? Bob Richmond Samurai Pathologist Maryville TN From rsrichmond <@t> gmail.com Sat Dec 6 16:50:58 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Dec 6 16:51:05 2014 Subject: [Histonet] Re: Histonet Digest, Vol 133, Issue 8 In-Reply-To: <5483443c.e179b60a.031d.69d0SMTPIN_ADDED_MISSING@mx.google.com> References: <5483443c.e179b60a.031d.69d0SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Evelyne Kokoskin at PublicHealthOntario asks: >>We are looking at infectious agents in thin blood smears and how to eliminate them before staining using fixation. Other than methanol, what are other fixatives that could possibly be used for fixing thin films ( that would destroy infectious agents) and allow staining with Giemsa stain.<< By "destroying them" I suppose you mean rendering them non-infectious, rather than removing them. I would think that the methanol in the Giemsa stain would pretty well accomplish that. But you'd need to find out what the regulatory agencies in Canada have decided. Bob Richmond Samurai Pathologist Maryville, Tennessee On Sat, Dec 6, 2014 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Block and Slide Retention (Blake Taylor) > 2. Blood smear question (Evelyne Kokoskin) > 3. Histology Openings in California (Taylor Rinaldi) > 4. Histology opening in Boulder, Colorado (Taylor Rinaldi) > 5. Re: Blood smear question (Rene J Buesa) > 6. Happy Holidays from RELIA Solutions 12-5-2014 Exciting New > Opportunities to Share!!! (Pam Barker) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 5 Dec 2014 18:06:29 +0000 > From: Blake Taylor > Subject: [Histonet] Block and Slide Retention > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > C69BCCA05D1B1341A3029022EC6EC8065C84447C@lmcmaildb01.lmc.lexhealth.org> > > Content-Type: text/plain; charset="us-ascii" > > We currently keep our blocks and slides indefinitely but would like to > revise our retention policy. I know CAP has a minimum of 10 years, Just > wanted some feedback on what others thoughts on this are. We are thinking > maybe 15-18years??? > > Blake Taylor > Surgical Pathology Supervisor > Lexington Medical Center > 803-936-8214 > bcdukes@lexhealth.org > > > PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments > are confidential property of the sender. The information is intended only > for the use of the person to whom it was addressed. Any other interception, > copying, accessing, or disclosure of this message is prohibited. The sender > takes no responsibility for any unauthorized reliance on this message. If > you have received this message in error, please immediately notify the > sender and purge the message you received. Do not forward this message > without permission. > > > > ------------------------------ > > Message: 2 > Date: Fri, 5 Dec 2014 18:30:11 +0000 > From: Evelyne Kokoskin > Subject: [Histonet] Blood smear question > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <2A63092EB60FF14D91637BA029A56E361DBF6281@OTO101PEX03V.oahpp.ca> > Content-Type: text/plain; charset="us-ascii" > > We are looking at infectious agents in thin blood smears and how to > eliminate them before staining using fixation. Other than methanol, what > are other fixatives that could possibly be used for fixing thin films ( > that would destroy infectious agents) and allow staining with Giemsa stain. > Thanks, > Evelyne > > > > > > ------------------------------ > > Message: 3 > Date: Fri, 5 Dec 2014 13:46:38 -0500 > From: "Taylor Rinaldi" > Subject: [Histonet] Histology Openings in California > To: > Message-ID: <041b01d010bb$cde833a0$69b89ae0$@prometheushealthcare.com> > Content-Type: text/plain; charset="us-ascii" > > A growing anatomic pathology laboratory in Modesto, CA is looking to hire > in > their Histology department. Bench and managerial positions available. ASCP > certification required. These are permanent, full time opportunities > offering relocation assistance. > > Please reach out to me for immediate consideration. > > Thanks so much! > > Taylor Rinaldi > > Recruiter > > Prometheus Healthcare > > Office (301) 693-9057 > > Taylor@prometheushealthcare.com > > > > ------------------------------ > > Message: 4 > Date: Fri, 5 Dec 2014 13:56:25 -0500 > From: "Taylor Rinaldi" > Subject: [Histonet] Histology opening in Boulder, Colorado > To: > Message-ID: <042801d010bd$2bd11350$837339f0$@prometheushealthcare.com> > Content-Type: text/plain; charset="us-ascii" > > A growing research laboratory in Boulder, Co is looking to hire in their > Histology department. They are looking a Histotechnician with at least 1-2 > years' experience. ASCP certification preferred, not required. This is a > permanent, full time opportunity. > > > > Please reach out to me for immediate consideration. > > Thanks so much! > > Taylor Rinaldi > > Recruiter > > Prometheus Healthcare > > Office (301) 693-9057 > > Taylor@prometheushealthcare.com > > > > > > ------------------------------ > > Message: 5 > Date: Fri, 5 Dec 2014 19:01:03 +0000 (UTC) > From: Rene J Buesa > Subject: Re: [Histonet] Blood smear question > To: Evelyne Kokoskin , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 1322250955.1563415.1417806064024.JavaMail.yahoo@jws10096.mail.ne1.yahoo.com > > > > Content-Type: text/plain; charset=UTF-8 > > Now you got me confused! If you are looking for infectious agents, why > would you like to destroy them?????Can you elaborate? > Ren? J. > > On Friday, December 5, 2014 1:30 PM, Evelyne Kokoskin < > Evelyne.Kokoskin@oahpp.ca> wrote: > > > We are looking at infectious agents in thin blood smears and how to > eliminate them before staining using fixation. Other than methanol, what > are other fixatives that could possibly be used for fixing thin films ( > that would destroy infectious agents) and allow staining with Giemsa stain. > Thanks, > Evelyne > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 6 > Date: Fri, 5 Dec 2014 14:15:00 -0500 (GMT-05:00) > From: Pam Barker > Subject: [Histonet] Happy Holidays from RELIA Solutions 12-5-2014 > Exciting New Opportunities to Share!!! > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > < > 15559281.1417806900944.JavaMail.root@wamui-hunyo.atl.sa.earthlink.net> > > Content-Type: text/plain; charset=UTF-8 > > Hello Histonetters!! > Happy Holidays!! > While a job change is the last thing on most people?s minds this time of > year; > May I take a minute of your time to ask; > Is a job change the next logical step in your career? > > This is a special bulletin for you. > Some of my best clients are expanding and as a result some fantastic > opportunities are open right now. > I wanted to touch base and give you a heads up. > Your next opportunity might be just around the corner > and > I might have it for you. > If you are looking for a position right now please contact me RIGHT AWAY!! > In addition to these opportunities I have new positions coming in DAILY!! > If you aren?t looking right away but want to let me know what would make a > perfect job for you so that I could keep an eye out that would be great too. > To do that, just shoot me an email at relia1@earthlink.net or call me > toll free at 866-607-3542. > > Here is a list of my current opportunities: > HISTOLOGY SUPERVISORS/MANAGERS: > Histology Supervisor - Modesto, CA > Histology Manager ? Flagstaff, AZ > AP Manager ? Hammond, IN > Lead Histology Tech ? St. Louis, MO > Asst. Supervisor ? Las Cruces, NM > Lead Histotech ? Yuma, AZ > Histology Supervisor ? Atlanta, GA > > HISTOTECHNICIANS/HISTOTECHNOLOGISTS: > Histology Tech ? Yuma, AZ > Histotechnician ? Hammond, IN > Histotech DAYS ? Modesto, CA > Histotech NIGHTS - Modesto, CA > Histotechnologist ? Atlanta, GA > Junior Histotech ? Boulder, CO > Grossing Tech ? Long Island, NY > Junior Histotech ? Boston, MA > > > > My clients offer competitive salaries, great benefits and in most cases > relocation assistance and or sign on bonuses. > **While all of these clients would LOVE to hire right away; they are also > willing to start the process with a phone interview now and continue the > process after the holidays if that would be better for you! > > Remember, I offer a 500.00 dollar referral fee if I place someone you > refer to me. > > I really appreciate you taking time out of your busy day to read my e-mail > Thanks Again! > > > Thanks-Pam > > > > Thank you! > > Pam M. Barker > President > RELIA > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: 407-657-2027 > Fax: 407-678-2788 > Cell: 407-353-5070 > e-mail: relia1@earthlink.net > Toll Free: 866-60-RELIA (866-607-3542) > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 133, Issue 8 > **************************************** > From joelleweaver <@t> hotmail.com Sun Dec 7 02:19:49 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Sun Dec 7 02:20:10 2014 Subject: [Histonet] Re: Block and Slide Retention In-Reply-To: References: Message-ID: Good point about lab closings and block/slide archives Dr. Richmond. I seem to remember a requirement for a policy for what a lab will do with their archives in the event of closing in the CAP checklist. Having a policy of course, is only part of the resolution. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Sat, 6 Dec 2014 17:46:11 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Block and Slide Retention > > Blake Taylor, Surgical Pathology Supervisor, Lexington Medical Center in SC > asks: > > >>We currently keep our blocks and slides indefinitely but would like to > revise our retention policy. I know CAP has a minimum of 10 years, Just > wanted some feedback on what others thoughts on this are. We are thinking > maybe 15-18 years???<< > > I think that with the rapid advent of molecular and genetic studies that > can be done on paraffin embedded tissue, that the 10 year minimum is going > to need to be extended, though I haven't heard of any proposals. A > non-trivial problem, because of the temperature control needed for storing > paraffin blocks. > > A related problem: what should be done with archived blocks when a lab > closes, as so many will in the next few years? > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Sun Dec 7 21:43:22 2014 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Sun Dec 7 21:43:28 2014 Subject: [Histonet] Please provide H&E protocol for 60um free-floating human brain sections Message-ID: Hello, Can someone please provide a H&E staining protocol for 60um free-floating human brainstem sections. Any assistance anyone can provide, I will be most appreciative. Happy Holiday & Regards Maria Mejia Histology Supervisor UCSF Department of Neurology From blayjorge <@t> gmail.com Mon Dec 8 01:42:14 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Mon Dec 8 01:42:21 2014 Subject: [Histonet] Instrumentation grants Message-ID: Dear Histonetters: Recently, I became aware of a research university that has been running expensive imaging instruments (e.g. electron microscopes) *without* service contracts for 2+ years, due to lack of funding. I have no idea what was the funding agency that allowed this institution to purchase the instruments (I suspect it was a US Federal agency) and when were the purchases made (I suspect the purchases were made at least 5 years ago). I have a few questions. Do you know of any unit that provides ongoing support for 1. Servicing instruments (?service contracts?, e.g. electron microscopes). 2. Maintaining facilities or centers containing such imaging instruments. If you have any suggestions, please email me directly at: blayjorge@gmail.com . Apologies for potential multiple emails you may receive. With gratefulness, Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From Toni.Rathborne <@t> rwjuh.edu Mon Dec 8 08:45:01 2014 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Mon Dec 8 08:45:09 2014 Subject: [Histonet] RE: Billing codes 2015 In-Reply-To: <712475632.10767980.1417786995288.JavaMail.zimbra@comcast.net> References: <389170114.10766209.1417786853795.JavaMail.zimbra@comcast.net> <712475632.10767980.1417786995288.JavaMail.zimbra@comcast.net> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24EECD8E@vap1013.win.rwjuh.edu> I found this in my inbox right after your email Marilynn. Thought it would be worth sharing with everyone. Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rmweber113@comcast.net Sent: Friday, December 05, 2014 8:43 AM To: histonet Subject: [Histonet] Billing codes 2015 Hi,? I was wondering if anyone has heard of any new billing codes for 2015 in regards to cytology,?urology pathology or immunohistochemistry. ?If so, could you please share? ? Thanks so much for your help. ? Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis <@t> che-east.org Mon Dec 8 08:53:16 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Mon Dec 8 08:53:20 2014 Subject: [Histonet] Vantage patient tissue and + control slide labels Message-ID: Hi folks, I am hoping to connect with somebody who has worked with Nexes (Ventana software),Vantage and CoPath. When the Vantage labels are printing we cannot tell the difference between the patient label and the positive control label. Our LIS wants us to use the exact staining protocol for our patients and controls but give a unique protocol number to the ones for positive controls. (Red flags are popping up all over for me on this idea) What do other labs do to handle this? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mjdessoye <@t> commonwealthhealth.net Mon Dec 8 12:40:51 2014 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael) Date: Mon Dec 8 12:42:22 2014 Subject: [Histonet] Environmental monitoring Message-ID: Hi All, Who do you like for environmental monitoring? I'm talking about in-lab testing, not employee badging. We have a vendor that performs formaldehyde, xylene, and acetic acid monitoring and we are considering a switch. Any input is appreciated. Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Smith_D <@t> kids.wustl.edu Mon Dec 8 14:53:28 2014 From: Smith_D <@t> kids.wustl.edu (Smith, Denise) Date: Mon Dec 8 14:53:50 2014 Subject: [Histonet] Angle of Wrist/Hands Using Microtome? Message-ID: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> Hi all! When I took HTL exam and there was a question on the exam that bothered me the most but I cannot find an exact answer for it, had to guess! I'm very curious about your thoughts on this. I have asked Histology Core and they weren't sure about it either - wanted to share it with you guys if you know the official answer for this! The question was mainly about the angle of wrist/hands on the rotating microtome and the degree must be correct so it won't cause any stress on wrist/hands, it listed 4 different choices: 1. 30 degrees wrist down forward 2. 90 degrees straight without bending the wrist 3. 60 degrees wrist up backward 4. 60 or 90 degrees wrist down forward (Please be gentle with me since I don't remember the EXACT numbers but those were close to numbers provided.) Thank you! Denise Smith The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From mtoole <@t> dcol.net Mon Dec 8 14:55:58 2014 From: mtoole <@t> dcol.net (Mike Toole) Date: Mon Dec 8 14:56:17 2014 Subject: [Histonet] RE: Environmental monitoring In-Reply-To: References: Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB335224A0364@mail> Mike, How does environmental monitoring differ from employee badging? Thanks, Mike Toole -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael Sent: Monday, December 08, 2014 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Environmental monitoring Hi All, Who do you like for environmental monitoring? I'm talking about in-lab testing, not employee badging. We have a vendor that performs formaldehyde, xylene, and acetic acid monitoring and we are considering a switch. Any input is appreciated. Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JSCHUMA1 <@t> Fairview.org Mon Dec 8 15:07:16 2014 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Mon Dec 8 15:07:26 2014 Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? In-Reply-To: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> References: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> Message-ID: <13399F5B2053F94EA80F3E8F4607A0FA0A180ABC@EXCH-PRIMARY-6.Fairview.org> I am not an ergonomic expert, and can't back this statement with fact, but I think that "without bending the wrist" is the key variable, as that is considered a "neutral" position that would not harm the employee. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise Sent: Monday, December 08, 2014 2:53 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Angle of Wrist/Hands Using Microtome? Hi all! When I took HTL exam and there was a question on the exam that bothered me the most but I cannot find an exact answer for it, had to guess! I'm very curious about your thoughts on this. I have asked Histology Core and they weren't sure about it either - wanted to share it with you guys if you know the official answer for this! The question was mainly about the angle of wrist/hands on the rotating microtome and the degree must be correct so it won't cause any stress on wrist/hands, it listed 4 different choices: 1. 30 degrees wrist down forward 2. 90 degrees straight without bending the wrist 3. 60 degrees wrist up backward 4. 60 or 90 degrees wrist down forward (Please be gentle with me since I don't remember the EXACT numbers but those were close to numbers provided.) Thank you! Denise Smith The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=e0qWO0MXWFiQgGWE0UmXdQ&r=LqDe7qpnDmZRCH58ZzBCJnXpTfDS3z3foGtV-fiTVCA&m=Clpb1HvNpog-dANduquNYBlFTUEo-dNuA1ENuNhnSbY&s=SYIz3MG9gPHETpWSg5HM3UHt3qP4J95h9zCphYvjHSE&e= From liz <@t> premierlab.com Mon Dec 8 15:32:57 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Dec 8 15:33:03 2014 Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? In-Reply-To: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> References: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDDB9@SBS2K8.premierlab.local> I think you want to keep your wrist straight so it would be 2. Just my two cents Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise Sent: Monday, December 08, 2014 1:53 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Angle of Wrist/Hands Using Microtome? Hi all! When I took HTL exam and there was a question on the exam that bothered me the most but I cannot find an exact answer for it, had to guess! I'm very curious about your thoughts on this. I have asked Histology Core and they weren't sure about it either - wanted to share it with you guys if you know the official answer for this! The question was mainly about the angle of wrist/hands on the rotating microtome and the degree must be correct so it won't cause any stress on wrist/hands, it listed 4 different choices: 1. 30 degrees wrist down forward 2. 90 degrees straight without bending the wrist 3. 60 degrees wrist up backward 4. 60 or 90 degrees wrist down forward (Please be gentle with me since I don't remember the EXACT numbers but those were close to numbers provided.) Thank you! Denise Smith The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Mon Dec 8 15:41:02 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Dec 8 15:41:07 2014 Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECDDB9@SBS2K8.premierlab.local> References: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> <14E2C6176416974295479C64A11CB9AE019C79ECDDB9@SBS2K8.premierlab.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E39172DC7BCB8@IBMB7Exchange.digestivespecialists.com> My two cents... # 2 Keep your wrist straight. (you may get rich if you get enough 2 cents!) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Monday, December 08, 2014 4:33 PM To: Smith, Denise; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? I think you want to keep your wrist straight so it would be 2. Just my two cents Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise Sent: Monday, December 08, 2014 1:53 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Angle of Wrist/Hands Using Microtome? Hi all! When I took HTL exam and there was a question on the exam that bothered me the most but I cannot find an exact answer for it, had to guess! I'm very curious about your thoughts on this. I have asked Histology Core and they weren't sure about it either - wanted to share it with you guys if you know the official answer for this! The question was mainly about the angle of wrist/hands on the rotating microtome and the degree must be correct so it won't cause any stress on wrist/hands, it listed 4 different choices: 1. 30 degrees wrist down forward 2. 90 degrees straight without bending the wrist 3. 60 degrees wrist up backward 4. 60 or 90 degrees wrist down forward (Please be gentle with me since I don't remember the EXACT numbers but those were close to numbers provided.) Thank you! Denise Smith The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Mon Dec 8 15:47:00 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon Dec 8 15:47:13 2014 Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39172DC7BCB8@IBMB7Exchange.digestivespecialists.com> References: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> <14E2C6176416974295479C64A11CB9AE019C79ECDDB9@SBS2K8.premierlab.local> <5A2BD13465E061429D6455C8D6B40E39172DC7BCB8@IBMB7Exchange.digestivespecialists.com> Message-ID: OSHA states "maintain neutral wrist and arm when working; work with their wrists in a neutral or straight" William DeSalvo william.desalvo@sonoraquest.ccom 602-768-3692 Sent from my iPhone > On Dec 8, 2014, at 2:41 PM, Blazek, Linda wrote: > > My two cents... # 2 Keep your wrist straight. > (you may get rich if you get enough 2 cents!) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala > Sent: Monday, December 08, 2014 4:33 PM > To: Smith, Denise; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? > > I think you want to keep your wrist straight so it would be 2. Just my two cents > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise > Sent: Monday, December 08, 2014 1:53 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Angle of Wrist/Hands Using Microtome? > > Hi all! > > When I took HTL exam and there was a question on the exam that bothered me the most but I cannot find an exact answer for it, had to guess! I'm very curious about your thoughts on this. I have asked Histology Core and they weren't sure about it either - wanted to share it with you guys if you know the official answer for this! > > The question was mainly about the angle of wrist/hands on the rotating microtome and the degree must be correct so it won't cause any stress on wrist/hands, it listed 4 different choices: > > > 1. 30 degrees wrist down forward > > 2. 90 degrees straight without bending the wrist > > 3. 60 degrees wrist up backward > > 4. 60 or 90 degrees wrist down forward > > > (Please be gentle with me since I don't remember the EXACT numbers but those were close to numbers provided.) > > Thank you! > > Denise Smith > > > The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Mon Dec 8 15:59:57 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Dec 8 16:00:34 2014 Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? In-Reply-To: References: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> <14E2C6176416974295479C64A11CB9AE019C79ECDDB9@SBS2K8.premierlab.local> <5A2BD13465E061429D6455C8D6B40E39172DC7BCB8@IBMB7Exchange.digestivespecialists.com> Message-ID: <40298d1d9b4746e583a118c5db224d3b@MAIL13M2N2.ad.uams.edu> One of our histologist uses a tennis ball on the end of his handle on the fly wheel. He says it helps him keep his wrist level. I can see it allows him to use less reach with his hand and his wrist is straighter than most. I really need to try this myself someday. I was trained wrist straight as possible and neutral if you wanted to avoid problems with you wrist and arm. The automated microtomes are great for facing blocks as it cuts the shoulder action as well as lower arm. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, December 08, 2014 3:47 PM To: Blazek, Linda Cc: histonet@lists.utsouthwestern.edu; Smith, Denise Subject: Re: [Histonet] RE: Angle of Wrist/Hands Using Microtome? OSHA states "maintain neutral wrist and arm when working; work with their wrists in a neutral or straight" William DeSalvo william.desalvo@sonoraquest.ccom 602-768-3692 Sent from my iPhone > On Dec 8, 2014, at 2:41 PM, Blazek, Linda wrote: > > My two cents... # 2 Keep your wrist straight. > (you may get rich if you get enough 2 cents!) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala > Sent: Monday, December 08, 2014 4:33 PM > To: Smith, Denise; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? > > I think you want to keep your wrist straight so it would be 2. Just my two cents > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise > Sent: Monday, December 08, 2014 1:53 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Angle of Wrist/Hands Using Microtome? > > Hi all! > > When I took HTL exam and there was a question on the exam that bothered me the most but I cannot find an exact answer for it, had to guess! I'm very curious about your thoughts on this. I have asked Histology Core and they weren't sure about it either - wanted to share it with you guys if you know the official answer for this! > > The question was mainly about the angle of wrist/hands on the rotating microtome and the degree must be correct so it won't cause any stress on wrist/hands, it listed 4 different choices: > > > 1. 30 degrees wrist down forward > > 2. 90 degrees straight without bending the wrist > > 3. 60 degrees wrist up backward > > 4. 60 or 90 degrees wrist down forward > > > (Please be gentle with me since I don't remember the EXACT numbers but those were close to numbers provided.) > > Thank you! > > Denise Smith > > > The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From bcooper <@t> chla.usc.edu Mon Dec 8 16:21:07 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Mon Dec 8 16:21:14 2014 Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? In-Reply-To: <40298d1d9b4746e583a118c5db224d3b@MAIL13M2N2.ad.uams.edu> References: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> <14E2C6176416974295479C64A11CB9AE019C79ECDDB9@SBS2K8.premierlab.local> <5A2BD13465E061429D6455C8D6B40E39172DC7BCB8@IBMB7Exchange.digestivespecialists.com> <40298d1d9b4746e583a118c5db224d3b@MAIL13M2N2.ad.uams.edu> Message-ID: The tennis ball is a good idea--may need to try that. I love hearing tales of these equipment "modifications"! Slightly off topic, but one of the techs who trained me kept a spark plug socket over the lever of the cassette clamp. The added leverage made it easier for him to change blocks. Looked a little funny though! Brian CHLA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Monday, December 08, 2014 2:00 PM To: WILLIAM DESALVO; Blazek, Linda Cc: histonet@lists.utsouthwestern.edu; Smith, Denise Subject: RE: [Histonet] RE: Angle of Wrist/Hands Using Microtome? One of our histologist uses a tennis ball on the end of his handle on the fly wheel. He says it helps him keep his wrist level. I can see it allows him to use less reach with his hand and his wrist is straighter than most. I really need to try this myself someday. I was trained wrist straight as possible and neutral if you wanted to avoid problems with you wrist and arm. The automated microtomes are great for facing blocks as it cuts the shoulder action as well as lower arm. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, December 08, 2014 3:47 PM To: Blazek, Linda Cc: histonet@lists.utsouthwestern.edu; Smith, Denise Subject: Re: [Histonet] RE: Angle of Wrist/Hands Using Microtome? OSHA states "maintain neutral wrist and arm when working; work with their wrists in a neutral or straight" William DeSalvo william.desalvo@sonoraquest.ccom 602-768-3692 Sent from my iPhone > On Dec 8, 2014, at 2:41 PM, Blazek, Linda wrote: > > My two cents... # 2 Keep your wrist straight. > (you may get rich if you get enough 2 cents!) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala > Sent: Monday, December 08, 2014 4:33 PM > To: Smith, Denise; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? > > I think you want to keep your wrist straight so it would be 2. Just my two cents > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Denise > Sent: Monday, December 08, 2014 1:53 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Angle of Wrist/Hands Using Microtome? > > Hi all! > > When I took HTL exam and there was a question on the exam that bothered me the most but I cannot find an exact answer for it, had to guess! I'm very curious about your thoughts on this. I have asked Histology Core and they weren't sure about it either - wanted to share it with you guys if you know the official answer for this! > > The question was mainly about the angle of wrist/hands on the rotating microtome and the degree must be correct so it won't cause any stress on wrist/hands, it listed 4 different choices: > > > 1. 30 degrees wrist down forward > > 2. 90 degrees straight without bending the wrist > > 3. 60 degrees wrist up backward > > 4. 60 or 90 degrees wrist down forward > > > (Please be gentle with me since I don't remember the EXACT numbers but those were close to numbers provided.) > > Thank you! > > Denise Smith > > > The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Smith_D <@t> kids.wustl.edu Tue Dec 9 07:11:26 2014 From: Smith_D <@t> kids.wustl.edu (Smith, Denise) Date: Tue Dec 9 07:14:24 2014 Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? In-Reply-To: References: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> <14E2C6176416974295479C64A11CB9AE019C79ECDDB9@SBS2K8.premierlab.local> <5A2BD13465E061429D6455C8D6B40E39172DC7BCB8@IBMB7Exchange.digestivespecialists.com> <40298d1d9b4746e583a118c5db224d3b@MAIL13M2N2.ad.uams.edu> Message-ID: <7578651EA53E304B96B06AD6D7B8992C2026C7F5@pcfmbx01.wusm-pcf.wustl.edu> Hi all, I would like to thank you for taking your time answering my question regarding the angle of wrist/hands using microtome! I thought I was wrong in first place using straight or neutral wrist, apparently not! Using tennis ball on the end of the handle on the fly wheel isn't a bad idea - will have to give it a try! Interesting modification on using spark plug socket over the level of the cassette clamp to make it easier to change blocks, never heard of that! Thank you all again!! Denise Smith -----Original Message----- From: Cooper, Brian [mailto:bcooper@chla.usc.edu] Sent: Monday, December 08, 2014 4:21 PM To: Marcum, Pamela A; WILLIAM DESALVO; Blazek, Linda Cc: histonet@lists.utsouthwestern.edu; Smith, Denise Subject: RE: [Histonet] RE: Angle of Wrist/Hands Using Microtome? The tennis ball is a good idea--may need to try that. I love hearing tales of these equipment "modifications"! Slightly off topic, but one of the techs who trained me kept a spark plug socket over the lever of the cassette clamp. The added leverage made it easier for him to change blocks. Looked a little funny though! Brian CHLA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Monday, December 08, 2014 2:00 PM To: WILLIAM DESALVO; Blazek, Linda Cc: histonet@lists.utsouthwestern.edu; Smith, Denise Subject: RE: [Histonet] RE: Angle of Wrist/Hands Using Microtome? One of our histologist uses a tennis ball on the end of his handle on the fly wheel. He says it helps him keep his wrist level. I can see it allows him to use less reach with his hand and his wrist is straighter than most. I really need to try this myself someday. I was trained wrist straight as possible and neutral if you wanted to avoid problems with you wrist and arm. The automated microtomes are great for facing blocks as it cuts the shoulder action as well as lower arm. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, December 08, 2014 3:47 PM To: Blazek, Linda Cc: histonet@lists.utsouthwestern.edu; Smith, Denise Subject: Re: [Histonet] RE: Angle of Wrist/Hands Using Microtome? OSHA states "maintain neutral wrist and arm when working; work with their wrists in a neutral or straight" William DeSalvo william.desalvo@sonoraquest.ccom 602-768-3692 Sent from my iPhone > On Dec 8, 2014, at 2:41 PM, Blazek, Linda wrote: > > My two cents... # 2 Keep your wrist straight. > (you may get rich if you get enough 2 cents!) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Elizabeth Chlipala > Sent: Monday, December 08, 2014 4:33 PM > To: Smith, Denise; 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] RE: Angle of Wrist/Hands Using Microtome? > > I think you want to keep your wrist straight so it would be 2. Just my two cents > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, > Denise > Sent: Monday, December 08, 2014 1:53 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Angle of Wrist/Hands Using Microtome? > > Hi all! > > When I took HTL exam and there was a question on the exam that bothered me the most but I cannot find an exact answer for it, had to guess! I'm very curious about your thoughts on this. I have asked Histology Core and they weren't sure about it either - wanted to share it with you guys if you know the official answer for this! > > The question was mainly about the angle of wrist/hands on the rotating microtome and the degree must be correct so it won't cause any stress on wrist/hands, it listed 4 different choices: > > > 1. 30 degrees wrist down forward > > 2. 90 degrees straight without bending the wrist > > 3. 60 degrees wrist up backward > > 4. 60 or 90 degrees wrist down forward > > > (Please be gentle with me since I don't remember the EXACT numbers but > those were close to numbers provided.) > > Thank you! > > Denise Smith > > > The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From mcauliff <@t> rwjms.rutgers.edu Tue Dec 9 08:41:51 2014 From: mcauliff <@t> rwjms.rutgers.edu (Geoff) Date: Tue Dec 9 08:42:05 2014 Subject: [Histonet] Angle of Wrist/Hands Using Microtome? In-Reply-To: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> References: <7578651EA53E304B96B06AD6D7B8992C2026A73C@pcfmbx01.wusm-pcf.wustl.edu> Message-ID: <54870A2F.5000306@umdnj.edu> From the point of view of anatomy of the wrist and potential for stress on the tendons in the carpal tunnel, gotta be #2. Geoff On 12/8/2014 3:53 PM, Smith, Denise wrote: > Hi all! > > When I took HTL exam and there was a question on the exam that bothered me the most but I cannot find an exact answer for it, had to guess! I'm very curious about your thoughts on this. I have asked Histology Core and they weren't sure about it either - wanted to share it with you guys if you know the official answer for this! > > The question was mainly about the angle of wrist/hands on the rotating microtome and the degree must be correct so it won't cause any stress on wrist/hands, it listed 4 different choices: > > > 1. 30 degrees wrist down forward > > 2. 90 degrees straight without bending the wrist > > 3. 60 degrees wrist up backward > > 4. 60 or 90 degrees wrist down forward > > > (Please be gentle with me since I don't remember the EXACT numbers but those were close to numbers provided.) > > Thank you! > > Denise Smith > > > The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff@rwjms.rutgers.edu ********************************************** From csorrell <@t> skindxgroup.com Tue Dec 9 17:01:40 2014 From: csorrell <@t> skindxgroup.com (Casey Sorrell) Date: Tue Dec 9 17:01:48 2014 Subject: [Histonet] Manager Opportunity - Birmingham, AL Message-ID: Skin Diagnostics Group, PC, is currently accepting candidate resumes for a Dermatopathology Laboratory Manager. POSITION TITLE: Manager of Lab Operations LOCATION: Birmingham, AL To apply: Please send resume to amoncrief@skindxgroup.com Job Summary: Manage 25+ Lab Technicians and Lab Assistants (2 shifts) in a high volume, national skin pathology laboratory. Maintain accreditations with CLIA, College of American Pathologists (CAP). This individual will use their abilities to motivate a staff to insure that high quality, cost effective services are consistently delivered and will be accountable for daily lab operational management, training, goal setting, prioritization, quality & process improvement, instrument evaluation/maintenance, and customer service. Qualifications: BS degree preferred, HT (ASCP), 5+ years experience in high volume Anatomic Pathology laboratory, preferably Dermatopathology, with minimum 3 years of successful supervisory/management experience. Excellent computer skills along with strong leadership ability, problem solving and resolution, teamwork, and excellent communication skills. Salary: Highly competitive compensation and benefits with relocation assistance. K. Casey Sorrell Chief Operating Officer 3512 Old Montgomery Highway Birmingham, AL 35209 205-879-2260 www.skindxgroup.com From abtdhu <@t> gmail.com Wed Dec 10 08:25:17 2014 From: abtdhu <@t> gmail.com (Dorothy Hu) Date: Wed Dec 10 08:25:21 2014 Subject: [Histonet] cutting MMA cross section of mouse femur Message-ID: Dear Histonetters, I normally use 85/15 ratio of MMA/dibutyl phthalate (DP) in my mouse tibia and femur longitudinal sections. But for cross section of mouse femur. Do you change the ratio of MMA/DP to make block more soft or hard? It is difficulty to get good cross sections. Any expert especially from hard tissue committee, please help. I'm very much appreciated. Dorothy Hu MGH endocrine From dmecham <@t> copc.net Wed Dec 10 09:28:36 2014 From: dmecham <@t> copc.net (Joseph Mecham) Date: Wed Dec 10 09:28:43 2014 Subject: [Histonet] Difference Between Histotech Lead and Histology Supervisor Positions Message-ID: I am looking for some opinions on how everyone's individual labs view the Lead tech and Histology Supervisor positions. Would love everyone's input. I know NSH's salary scale shows a larger variance in pay between the two. Thanks in advance! Drew Mecham Central Oregon Regional Pathology Services 1348 NE Cushing Drive Bend, OR 97701 541.693.2651 From akbitting <@t> geisinger.edu Wed Dec 10 10:45:03 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Wed Dec 10 10:45:40 2014 Subject: [Histonet] shelf life Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7BD983@GHSEXMBX1W8K1V.geisinger.edu> We are being inspected soon and I'm trying to check the labels on all of my chemicals. How do I determine the expiration date of a 1N HCl solution I prepared in house. I went on line and a commercially available 1N HCl solution has a 36 month shelf life according to the vendor's web site. I don't know how they arrived at that. I know concentrated HCl has an indefinite shelf life, but a 5 year retest date appears on the COA. It's confusing to me. Any help would be appreciated. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From Timothy.Morken <@t> ucsf.edu Wed Dec 10 11:00:14 2014 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Dec 10 11:00:24 2014 Subject: [Histonet] RE: shelf life In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F69C7BD983@GHSEXMBX1W8K1V.geisinger.edu> References: <77F52EFAB8B1694B885E277C48FCD0F69C7BD983@GHSEXMBX1W8K1V.geisinger.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF367BD824@ex07.net.ucsf.edu> Angela most of these "shelf life" dates are just the length of time a vendor is willing to be responsible for the reagent. It's mostly a financial decision, not chemical deterioration. They just don't want to be responsible for chemicals for too long. So that is why you see variations in shelf life or expiration dates for the same chemical from different vendors. We generally give any solution we make up a 6-month shelf life unless we know from validations that is it is shorter. We make up solutions in amounts that will be used up before that date so we never have expired reagents around. That's easier than always having to inventory for expired reagents. To justify your dating you can use published dates from various vendors (ie, datasheets, expirations on the bottle, etc). For chemicals that do not state expiration dates I actually asked the question and got a written reply (via their website) from Fisher that states liquid chemicals are good for 3 years and dry chemicals for 5 years unless stated on the bottle. I put that letter in our manual as documentation on how we arrived at our in-house expiration dating. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, December 10, 2014 8:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shelf life We are being inspected soon and I'm trying to check the labels on all of my chemicals. How do I determine the expiration date of a 1N HCl solution I prepared in house. I went on line and a commercially available 1N HCl solution has a 36 month shelf life according to the vendor's web site. I don't know how they arrived at that. I know concentrated HCl has an indefinite shelf life, but a 5 year retest date appears on the COA. It's confusing to me. Any help would be appreciated. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HernandezJW <@t> uthscsa.edu Wed Dec 10 11:00:49 2014 From: HernandezJW <@t> uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Wed Dec 10 11:01:01 2014 Subject: [Histonet] Solution Chemistry - Alcoholic Formalin - Bone Histology - Fluorescent Label Message-ID: I was going to make a new Alcoholic Formalin Solution. Using the following recipe/ratio: 2700mL 95% EtOH 905mL DI H2O 95mL Formalin (37-40%) Instead of using 37-40% Formalin, I am going to use a 10% buffered formalin solution containing the following chemicals/buffers: Formaldehyde 36.5%, 10% V/V Sodium Phosphate Monobasic, 0.4% W/V Sodium Phosphate Dibasic, 0.65% W/V Deionized Water <100% V/V Since it is buffered already, I will be adding only 27mL of (10% Formalin) to 73mL (95% EtOH). The solution is to be used to fix tetracycline florescent labels within bone tissue. My question is if this concentration will work and will the buffers interfere with the fixation procedure? Regards, Jesse H. From rjbuesa <@t> yahoo.com Wed Dec 10 11:25:58 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 10 11:26:04 2014 Subject: [Histonet] shelf life In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F69C7BD983@GHSEXMBX1W8K1V.geisinger.edu> References: <77F52EFAB8B1694B885E277C48FCD0F69C7BD983@GHSEXMBX1W8K1V.geisinger.edu> Message-ID: <1619053664.4888716.1418232358223.JavaMail.yahoo@jws100110.mail.ne1.yahoo.com> Expiration dates and not only confusing but very variable and inaccurate. I do not think you should worry about how the vendor determined the exp. date?maybe it is just greater than a competitor's just to "lure" you to buy theirs, who knows, but you can?use their exp.date for yours.HCl absorbs water vapor?if the container?is not well capped, and that may be the "over-reacting" recommendation of testing is in 5 years, but it could be 4 years or 10 years depending in the environment humidity or the cap used.These regulations?are something like the "piggy banks", they make "parents bank robbers" and the exp.dates "make histotechs liers".With experience you will be able to determine that some solutions "weaken" after some time lapse, use those known lapses as your exp.date for those solutions and "expand" to others.The inspectors, in their "infinite wisdom" want to read a date, regarldess of its accuracy. Their?"ego" is saved by a written date.Ren? J.? On Wednesday, December 10, 2014 11:46 AM, "Bitting, Angela K." wrote: We are being inspected soon and I'm trying to check the labels on all of my chemicals.? How do I determine the expiration date of a 1N HCl solution I prepared in house.? I went on line and a commercially available 1N HCl solution has a 36 month shelf life according to the vendor's web site. I don't know how they arrived at that. I know concentrated HCl has an indefinite shelf life, but a 5 year retest date appears on the COA. It's confusing to me. Any help would be appreciated. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jobaluyut <@t> gmail.com Wed Dec 10 23:18:41 2014 From: jobaluyut <@t> gmail.com (Joanna) Date: Wed Dec 10 23:18:53 2014 Subject: [Histonet] Gomori Trichrome Message-ID: <66D86405-5915-4D8B-93CC-D8125B122385@gmail.com> Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada From Erin.Martin <@t> ucsf.edu Thu Dec 11 04:37:37 2014 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Dec 11 04:37:47 2014 Subject: [Histonet] Lab power failures Message-ID: <24B7B291CC88D04AB663958E77A1F59D1D0D1E@ex09.net.ucsf.edu> Good morning all! Would anyone mind sharing their plans for power failure? We have backup generator power on red plugs but my pathologists want a plan for what we would do if the power went out AND the backup generator failed. Thanks for your help! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From Sidonie.Aten <@t> hcahealthcare.com Thu Dec 11 10:15:00 2014 From: Sidonie.Aten <@t> hcahealthcare.com (Sidonie.Aten@hcahealthcare.com) Date: Thu Dec 11 10:15:22 2014 Subject: [Histonet] IHC CONTROL LOG Message-ID: <8E35B71D4C94C14BA935DAC7B93B4670AAE9@XRDCWPMSGHCMD2C.hca.corpad.net> BEEN DOING IHC FOR TWO YEARS. WOULD LIKE TO KNOW HOW OTHERS ARE LOGGING THEIR CONTROL CASES. THANKS, SIDONIE From Timothy.Morken <@t> ucsf.edu Thu Dec 11 10:18:39 2014 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Dec 11 10:21:36 2014 Subject: [Histonet] Gomori Trichrome In-Reply-To: <66D86405-5915-4D8B-93CC-D8125B122385@gmail.com> References: <66D86405-5915-4D8B-93CC-D8125B122385@gmail.com> Message-ID: <761E2B5697F795489C8710BCC72141FF367BF1C2@ex07.net.ucsf.edu> Joanna, If it looks like it is under the section, then I would suspect trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanna Sent: Wednesday, December 10, 2014 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmmathis <@t> wakehealth.edu Thu Dec 11 12:26:07 2014 From: cmmathis <@t> wakehealth.edu (Cathy M. Mathis/Regenerative Medicine) Date: Thu Dec 11 12:26:12 2014 Subject: [Histonet] RE: Histonet Digest, Vol 133, Issue 13 In-Reply-To: References: Message-ID: <4816F916B11AEC4E92A0D01372F573E2805004BF@EXCHDB8.medctr.ad.wfubmc.edu> Are you filtering your trichrome stain just before using it? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, December 11, 2014 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 133, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Gomori Trichrome (Joanna) 2. Lab power failures (Martin, Erin) 3. IHC CONTROL LOG (Sidonie.Aten@hcahealthcare.com) 4. RE: Gomori Trichrome (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Wed, 10 Dec 2014 21:18:41 -0800 From: Joanna Subject: [Histonet] Gomori Trichrome To: "histonet@lists.utsouthwestern.edu" Message-ID: <66D86405-5915-4D8B-93CC-D8125B122385@gmail.com> Content-Type: text/plain; charset=us-ascii Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada ------------------------------ Message: 2 Date: Thu, 11 Dec 2014 10:37:37 +0000 From: "Martin, Erin" Subject: [Histonet] Lab power failures To: histonet Message-ID: <24B7B291CC88D04AB663958E77A1F59D1D0D1E@ex09.net.ucsf.edu> Content-Type: text/plain; charset="iso-8859-1" Good morning all! Would anyone mind sharing their plans for power failure? We have backup generator power on red plugs but my pathologists want a plan for what we would do if the power went out AND the backup generator failed. Thanks for your help! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 3 Date: Thu, 11 Dec 2014 16:15:00 +0000 From: Subject: [Histonet] IHC CONTROL LOG To: Message-ID: <8E35B71D4C94C14BA935DAC7B93B4670AAE9@XRDCWPMSGHCMD2C.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" BEEN DOING IHC FOR TWO YEARS. WOULD LIKE TO KNOW HOW OTHERS ARE LOGGING THEIR CONTROL CASES. THANKS, SIDONIE ------------------------------ Message: 4 Date: Thu, 11 Dec 2014 16:18:39 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] Gomori Trichrome To: 'Joanna' , "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF367BF1C2@ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Joanna, If it looks like it is under the section, then I would suspect trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanna Sent: Wednesday, December 10, 2014 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 133, Issue 13 ***************************************** From cmmathis <@t> wakehealth.edu Thu Dec 11 12:32:46 2014 From: cmmathis <@t> wakehealth.edu (Cathy M. Mathis/Regenerative Medicine) Date: Thu Dec 11 12:32:52 2014 Subject: [Histonet] RE: Gomori Trichrome Message-ID: <4816F916B11AEC4E92A0D01372F573E2805004DC@EXCHDB8.medctr.ad.wfubmc.edu> Have you tried filtering the stain just before using it? Cathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, December 11, 2014 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 133, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Gomori Trichrome (Joanna) 2. Lab power failures (Martin, Erin) 3. IHC CONTROL LOG (Sidonie.Aten@hcahealthcare.com) 4. RE: Gomori Trichrome (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Wed, 10 Dec 2014 21:18:41 -0800 From: Joanna Subject: [Histonet] Gomori Trichrome To: "histonet@lists.utsouthwestern.edu" Message-ID: <66D86405-5915-4D8B-93CC-D8125B122385@gmail.com> Content-Type: text/plain; charset=us-ascii Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada ------------------------------ Message: 2 Date: Thu, 11 Dec 2014 10:37:37 +0000 From: "Martin, Erin" Subject: [Histonet] Lab power failures To: histonet Message-ID: <24B7B291CC88D04AB663958E77A1F59D1D0D1E@ex09.net.ucsf.edu> Content-Type: text/plain; charset="iso-8859-1" Good morning all! Would anyone mind sharing their plans for power failure? We have backup generator power on red plugs but my pathologists want a plan for what we would do if the power went out AND the backup generator failed. Thanks for your help! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 3 Date: Thu, 11 Dec 2014 16:15:00 +0000 From: Subject: [Histonet] IHC CONTROL LOG To: Message-ID: <8E35B71D4C94C14BA935DAC7B93B4670AAE9@XRDCWPMSGHCMD2C.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" BEEN DOING IHC FOR TWO YEARS. WOULD LIKE TO KNOW HOW OTHERS ARE LOGGING THEIR CONTROL CASES. THANKS, SIDONIE ------------------------------ Message: 4 Date: Thu, 11 Dec 2014 16:18:39 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] Gomori Trichrome To: 'Joanna' , "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF367BF1C2@ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Joanna, If it looks like it is under the section, then I would suspect trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanna Sent: Wednesday, December 10, 2014 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 133, Issue 13 ***************************************** From Sarah.Lewis <@t> nationwidechildrens.org Thu Dec 11 13:17:54 2014 From: Sarah.Lewis <@t> nationwidechildrens.org (Lewis, Sarah) Date: Thu Dec 11 13:18:03 2014 Subject: [Histonet] Gomori Trichrome In-Reply-To: <761E2B5697F795489C8710BCC72141FF367BF1C2@ex07.net.ucsf.edu> References: <66D86405-5915-4D8B-93CC-D8125B122385@gmail.com> <761E2B5697F795489C8710BCC72141FF367BF1C2@ex07.net.ucsf.edu> Message-ID: <4BAB3E4AF573804EA9929EFD1E15817124FB0C3C@RESEXCHMBX01.CRII.ORG> Joanna, I agree with Tim... We also see this at times if the tissue has freezing artifact. Gomori Trichrome is very unforgiving :) Sarah E. Lewis HT, ASCP The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Thursday, December 11, 2014 11:19 AM To: 'Joanna'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gomori Trichrome Joanna, If it looks like it is under the section, then I would suspect trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v2/url?u=http-3A__mailto-3Ahistonet-2Dbounces-40lists.utsouthwestern.edu&d=AAIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=7bt8tbqem5cRuX3hC3eOeQ&m=0vo4hfofYzOL04nyQnusz7sZCdl6SlMBYQxPhvKuLTo&s=9bOOBwtwMikwEx-4zwlWv08DLvU_t-ipNcKOpMi5GfA&e= ] On Behalf Of Joanna Sent: Wednesday, December 10, 2014 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AAIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=7bt8tbqem5cRuX3hC3eOeQ&m=0vo4hfofYzOL04nyQnusz7sZCdl6SlMBYQxPhvKuLTo&s=j_zGkNIiR0dF7a9QsR9pyFxqkZn_Q4JNpgF8pigo2UU&e= From CDavis <@t> che-east.org Thu Dec 11 13:56:32 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu Dec 11 13:56:38 2014 Subject: [Histonet] RE: Lab power failures Message-ID: Message: 2 Date: Thu, 11 Dec 2014 10:37:37 +0000 From: "Martin, Erin" Subject: [Histonet] Lab power failures To: histonet Message-ID: <24B7B291CC88D04AB663958E77A1F59D1D0D1E@ex09.net.ucsf.edu> Content-Type: text/plain; charset="iso-8859-1" Good morning all! Would anyone mind sharing their plans for power failure? We have backup generator power on red plugs but my pathologists want a plan for what we would do if the power went out AND the backup generator failed. Thanks for your help! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Erin, "if the power went out AND the backup generator" , My question is under what circumstances are they expecting this to happen? That being said and not enough information, best answer would be send to another lab for processing to at least protect the integrity of the tissue for future testing until the electricity issue is resolved.... No electricity means no lights, fume hoods, so hand processing is out and you should not let the tissue sit in solutions that might jeopardize future staining longer than recommendation (eg.Her2) and they should state any variances of normal process in the report. Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From raghulj <@t> orchidpharma.com Thu Dec 11 23:22:25 2014 From: raghulj <@t> orchidpharma.com (raghulj@orchidpharma.com) Date: Thu Dec 11 23:22:49 2014 Subject: [Histonet] Shelf life In-Reply-To: <8f8ec833e1cc4a079a82e82933617c9c@AM1FFO11FD049.protection.gbl> References: <8f8ec833e1cc4a079a82e82933617c9c@AM1FFO11FD049.protection.gbl> Message-ID: Hi Angela, We came across similar situation during our GLP inspection. As Morken has mentioned the validation would certainly stand by us, more than the expiry dates in the bottle. We give 3 months expiry for any reagents prepared in our lab and usually it get consumed by that time. I would suggest to fix a purpose for the prepared solution and validate it to the purpose, say by checking the pH when trying to use the HCl solution to differentiate. Hope this was of some help. All the best Regards raghul -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 10 December 2014 23:37 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 133, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Manager Opportunity - Birmingham, AL (Casey Sorrell) 2. cutting MMA cross section of mouse femur (Dorothy Hu) 3. Difference Between Histotech Lead and Histology Supervisor Positions (Joseph Mecham) 4. shelf life (Bitting, Angela K.) 5. RE: shelf life (Morken, Timothy) 6. Solution Chemistry - Alcoholic Formalin - Bone Histology - Fluorescent Label (Hernandez, Jesus Willibaldo) 7. Re: shelf life (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Tue, 9 Dec 2014 17:01:40 -0600 From: Casey Sorrell Subject: [Histonet] Manager Opportunity - Birmingham, AL To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Skin Diagnostics Group, PC, is currently accepting candidate resumes for a Dermatopathology Laboratory Manager. POSITION TITLE: Manager of Lab Operations LOCATION: Birmingham, AL To apply: Please send resume to amoncrief@skindxgroup.com Job Summary: Manage 25+ Lab Technicians and Lab Assistants (2 shifts) in a high volume, national skin pathology laboratory. Maintain accreditations with CLIA, College of American Pathologists (CAP). This individual will use their abilities to motivate a staff to insure that high quality, cost effective services are consistently delivered and will be accountable for daily lab operational management, training, goal setting, prioritization, quality & process improvement, instrument evaluation/maintenance, and customer service. Qualifications: BS degree preferred, HT (ASCP), 5+ years experience in high volume Anatomic Pathology laboratory, preferably Dermatopathology, with minimum 3 years of successful supervisory/management experience. Excellent computer skills along with strong leadership ability, problem solving and resolution, teamwork, and excellent communication skills. Salary: Highly competitive compensation and benefits with relocation assistance. K. Casey Sorrell Chief Operating Officer 3512 Old Montgomery Highway Birmingham, AL 35209 205-879-2260 www.skindxgroup.com ------------------------------ Message: 2 Date: Wed, 10 Dec 2014 09:25:17 -0500 From: Dorothy Hu Subject: [Histonet] cutting MMA cross section of mouse femur To: "histonet@lists.utsouthwestern.edu" Cc: Dorothy Hu Message-ID: Content-Type: text/plain; charset=UTF-8 Dear Histonetters, I normally use 85/15 ratio of MMA/dibutyl phthalate (DP) in my mouse tibia and femur longitudinal sections. But for cross section of mouse femur. Do you change the ratio of MMA/DP to make block more soft or hard? It is difficulty to get good cross sections. Any expert especially from hard tissue committee, please help. I'm very much appreciated. Dorothy Hu MGH endocrine ------------------------------ Message: 3 Date: Wed, 10 Dec 2014 15:28:36 +0000 From: Joseph Mecham Subject: [Histonet] Difference Between Histotech Lead and Histology Supervisor Positions To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for some opinions on how everyone's individual labs view the Lead tech and Histology Supervisor positions. Would love everyone's input. I know NSH's salary scale shows a larger variance in pay between the two. Thanks in advance! Drew Mecham Central Oregon Regional Pathology Services 1348 NE Cushing Drive Bend, OR 97701 541.693.2651 ------------------------------ Message: 4 Date: Wed, 10 Dec 2014 16:45:03 +0000 From: "Bitting, Angela K." Subject: [Histonet] shelf life To: "histonet@lists.utsouthwestern.edu" Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C7BD983@GHSEXMBX1W8K1V.geisinger.edu> Content-Type: text/plain; charset="us-ascii" We are being inspected soon and I'm trying to check the labels on all of my chemicals. How do I determine the expiration date of a 1N HCl solution I prepared in house. I went on line and a commercially available 1N HCl solution has a 36 month shelf life according to the vendor's web site. I don't know how they arrived at that. I know concentrated HCl has an indefinite shelf life, but a 5 year retest date appears on the COA. It's confusing to me. Any help would be appreciated. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------ Message: 5 Date: Wed, 10 Dec 2014 17:00:14 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: shelf life To: "'Bitting, Angela K.'" , "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF367BD824@ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Angela most of these "shelf life" dates are just the length of time a vendor is willing to be responsible for the reagent. It's mostly a financial decision, not chemical deterioration. They just don't want to be responsible for chemicals for too long. So that is why you see variations in shelf life or expiration dates for the same chemical from different vendors. We generally give any solution we make up a 6-month shelf life unless we know from validations that is it is shorter. We make up solutions in amounts that will be used up before that date so we never have expired reagents around. That's easier than always having to inventory for expired reagents. To justify your dating you can use published dates from various vendors (ie, datasheets, expirations on the bottle, etc). For chemicals that do not state expiration dates I actually asked the question and got a written reply (via their website) from Fisher that states liquid chemicals are good for 3 years and dry chemicals for 5 years unless stated on the bottle. I put that letter in our manual as documentation on how we arrived at our in-house expiration dating. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K. Sent: Wednesday, December 10, 2014 8:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shelf life We are being inspected soon and I'm trying to check the labels on all of my chemicals. How do I determine the expiration date of a 1N HCl solution I prepared in house. I went on line and a commercially available 1N HCl solution has a 36 month shelf life according to the vendor's web site. I don't know how they arrived at that. I know concentrated HCl has an indefinite shelf life, but a 5 year retest date appears on the COA. It's confusing to me. Any help would be appreciated. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 10 Dec 2014 17:00:49 +0000 From: "Hernandez, Jesus Willibaldo" Subject: [Histonet] Solution Chemistry - Alcoholic Formalin - Bone Histology - Fluorescent Label To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I was going to make a new Alcoholic Formalin Solution. Using the following recipe/ratio: 2700mL 95% EtOH 905mL DI H2O 95mL Formalin (37-40%) Instead of using 37-40% Formalin, I am going to use a 10% buffered formalin solution containing the following chemicals/buffers: Formaldehyde 36.5%, 10% V/V Sodium Phosphate Monobasic, 0.4% W/V Sodium Phosphate Dibasic, 0.65% W/V Deionized Water <100% V/V Since it is buffered already, I will be adding only 27mL of (10% Formalin) to 73mL (95% EtOH). The solution is to be used to fix tetracycline florescent labels within bone tissue. My question is if this concentration will work and will the buffers interfere with the fixation procedure? Regards, Jesse H. ------------------------------ Message: 7 Date: Wed, 10 Dec 2014 17:25:58 +0000 (UTC) From: Rene J Buesa Subject: Re: [Histonet] shelf life To: "Bitting, Angela K." , "histonet@lists.utsouthwestern.edu" Message-ID: <1619053664.4888716.1418232358223.JavaMail.yahoo@jws100110.mail.ne1.yahoo.com> Content-Type: text/plain; charset=UTF-8 Expiration dates and not only confusing but very variable and inaccurate. I do not think you should worry about how the vendor determined the exp. date???maybe it is just greater than a competitor's just to "lure" you to buy theirs, who knows, but you can??use their exp.date for yours.HCl absorbs water vapor??if the container??is not well capped, and that may be the "over-reacting" recommendation of testing is in 5 years, but it could be 4 years or 10 years depending in the environment humidity or the cap used.These regulations??are something like the "piggy banks", they make "parents bank robbers" and the exp.dates "make histotechs liers".With experience you will be able to determine that some solutions "weaken" after some time lapse, use those known lapses as your exp.date for those solutions and "expand" to others.The inspectors, in their "infinite wisdom" want to read a date, regarldess of its accuracy. Their??"ego" is saved by a written date.Ren?? J.?? On Wednesday, December 10, 2014 11:46 AM, "Bitting, Angela K." wrote: We are being inspected soon and I'm trying to check the labels on all of my chemicals.?? How do I determine the expiration date of a 1N HCl solution I prepared in house.?? I went on line and a commercially available 1N HCl solution has a 36 month shelf life according to the vendor's web site. I don't know how they arrived at that. I know concentrated HCl has an indefinite shelf life, but a 5 year retest date appears on the COA. It's confusing to me. Any help would be appreciated. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 133, Issue 12 ***************************************** THIS E-MAIL IS CONFIDENTIAL and intended solely for the use of the individual to whom it is addressed. If you are not the addressee, any disclosure, reproduction, copying, distribution, or other dissemination or use of this communication is strictly prohibited. Finally the recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email. From Colleen_Herring <@t> bshsi.org Fri Dec 12 07:16:18 2014 From: Colleen_Herring <@t> bshsi.org (Herring, Colleen) Date: Fri Dec 12 07:16:23 2014 Subject: [Histonet] Controls Message-ID: <3AE5857C7EC73F4E983F39C97DF811C018ECEC3E@EDC-EXMB4-01.ads.bshsi.com> Help! Running low on CMV and AFB positive control blocks. Looking into buying precut slides but the price is steep. Any suggestion ? Which company has the best price. ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From PAMarcum <@t> uams.edu Fri Dec 12 08:01:29 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Dec 12 08:01:36 2014 Subject: [Histonet] RE: Controls In-Reply-To: <3AE5857C7EC73F4E983F39C97DF811C018ECEC3E@EDC-EXMB4-01.ads.bshsi.com> References: <3AE5857C7EC73F4E983F39C97DF811C018ECEC3E@EDC-EXMB4-01.ads.bshsi.com> Message-ID: <3c6cf12fed4a489fa21f32b17f97f9f6@MAIL13M2N1.ad.uams.edu> If you require the tissue to be human in origin be careful as many of the companies are now using animal tissue for controls. This is a part of HIIPA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen Sent: Friday, December 12, 2014 7:16 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls Help! Running low on CMV and AFB positive control blocks. Looking into buying precut slides but the price is steep. Any suggestion ? Which company has the best price. ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From brett_connolly <@t> merck.com Fri Dec 12 09:06:13 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Dec 12 09:06:49 2014 Subject: [Histonet] CD31 IHC on bone Message-ID: Hi all, A colleague is having problems staining CD31 on bone sections and is looking for recommendations for specific antibodies and antigen retrieval methods. Can anyone help? Thanks, Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From andreahooper <@t> rocketmail.com Fri Dec 12 11:53:31 2014 From: andreahooper <@t> rocketmail.com (Andrea Hooper) Date: Fri Dec 12 11:53:36 2014 Subject: [Histonet] CD31 IHC on bone In-Reply-To: References: Message-ID: Human or mouse? Are they trying to stain bone vasculature? If so I can recommend superior markers. Andrea > On Dec 12, 2014, at 10:06 AM, Connolly, Brett M wrote: > > Hi all, > > A colleague is having problems staining CD31 on bone sections and is looking for recommendations for specific antibodies and antigen retrieval methods. > Can anyone help? > > Thanks, > Brett > > Brett M. Connolly, Ph.D. > Principle Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HernandezJW <@t> uthscsa.edu Fri Dec 12 12:23:31 2014 From: HernandezJW <@t> uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Fri Dec 12 12:23:36 2014 Subject: [Histonet] Alcohol Formalin Message-ID: Dear all, I have just started fixation of tetracycline bone tissue samples in Alcohol Formalin. My question is how long should samples be fixed with this solution type? Also, what are the next steps in processing the tissue? Here is my Alcohol Formalin Solution Breakdown: 2.7mL 10% NBF (Formalin concentration is 3.7%) 7.3mL 95% EtOH The ratio for this formula was derived from this original Alcohol Formalin Solution: 2700mL 95% EtOH 905mL DI H2O 95mL Formaldehyde (37-40% conc.) Regards, Jesse H. From bhartologist <@t> gmail.com Fri Dec 12 14:00:07 2014 From: bhartologist <@t> gmail.com (Bharti Parihar) Date: Fri Dec 12 14:00:11 2014 Subject: [Histonet] Slide Train Wreck Message-ID: Salutations Histonetters! Anyone have any ideas as to why the following may be happening? During a run on our Benchmarks, slides come off their pads and when we open them, it just looks like a train wreck. They've flown off their position and knocked into adjacent slides which then knock into other ones. Of course this often means we have to re-run some cases because it didn't stain properly, but surprisingly, many stain fine. I'm not sure why this happens. We initially thought it was due to the pads being too dry when starting our first run for the day, so we started wetting the pads down before loading slides onto them. This works most of the time but not always. Any thoughts? Thanks to everyone who replies! -- Bharti Parihar, HT (ASCP) From mpence <@t> grhs.net Fri Dec 12 14:10:52 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Dec 12 14:11:18 2014 Subject: [Histonet] Slide Train Wreck In-Reply-To: References: Message-ID: There can be a number of reasons for this. First, it can be that your "little" tabs are worn and not holding. Second, it could be that you have one of your jet dispensers putting to much solution on or a air nozzle blowing to hard lifting the slides. Third, the clearance between your slides and the passing nozzles is very little. This can catch the slides and push them. Lastly, your drain main be plugged and the liquid level in the pan can rise and float the slides from the heating pad. One thing to remember is that when you put liquid under the slide it raises the height of the slide and gets it closer to the moving parts overhead. Just some things I would be looking at. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bharti Parihar Sent: Friday, December 12, 2014 2:02 PM To: Histonet Archive Subject: [Histonet] Slide Train Wreck Salutations Histonetters! Anyone have any ideas as to why the following may be happening? During a run on our Benchmarks, slides come off their pads and when we open them, it just looks like a train wreck. They've flown off their position and knocked into adjacent slides which then knock into other ones. Of course this often means we have to re-run some cases because it didn't stain properly, but surprisingly, many stain fine. I'm not sure why this happens. We initially thought it was due to the pads being too dry when starting our first run for the day, so we started wetting the pads down before loading slides onto them. This works most of the time but not always. Any thoughts? Thanks to everyone who replies! -- Bharti Parihar, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmoreira <@t> sidra.org Sat Dec 13 22:36:44 2014 From: jmoreira <@t> sidra.org (Joana Moreira) Date: Sat Dec 13 22:36:57 2014 Subject: [Histonet] BoneSTATION - Milestone Message-ID: Hi everyone! I was wondering if there's anyone out there using the Milestone BoneSTATION for fixing and decalcifying bone tissue: http://www.milestonemedsrl.com/histopathology/products/pre-analytical-tools/bonestation.html Is it good? Not good? Easy to operate and maintain? Do you use it with in-house decalcifying solutions or commercial available ones? Have you tried their MOL-Decal solution on BMT? Any thoughts? What about the optimization and standardization of protocols? Is it quite straight forward? Or complicated / Difficult? We are considering it and any feedback would be much appreciated. Send me a PM if you like. Many Thanks, Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From HernandezJW <@t> uthscsa.edu Mon Dec 15 12:12:02 2014 From: HernandezJW <@t> uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Mon Dec 15 12:12:07 2014 Subject: [Histonet] Alcoholic Formalin Message-ID: Just looking to see if anyone has a recipe for this solution as well as processing times. I think you can dehydrate and fix at the same time so I just wanted to verify this with my fellow Histonetters. The application is for tetracycline bone tissue labeling /MMA embedding. Thank you. Regards, Jesse From thomas.huynh <@t> mdanderson.org Mon Dec 15 10:12:48 2014 From: thomas.huynh <@t> mdanderson.org (Huynh,Thomas) Date: Tue Dec 16 02:23:27 2014 Subject: [Histonet] Bone Station- Milestone Message-ID: Hi Joana, We are currently using the Milestone microwave to decal our bone marrow core biopsies (for faster TAT). We use our in-house formic acid (10%) and run it in the microwave for 2.5 to 3.00 hours with 50 degree Celsius cycle (you can tweak to where it works for your specimens). Assuming that they have been fixed well prior to decalcifying; personally, I would rather decal them the old fashion overnight but they always rush them nowadays. Thomas -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, December 14, 2014 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 133, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. BoneSTATION - Milestone (Joana Moreira) ---------------------------------------------------------------------- Message: 1 Date: Sun, 14 Dec 2014 04:36:44 +0000 From: Joana Moreira Subject: [Histonet] BoneSTATION - Milestone To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi everyone! I was wondering if there's anyone out there using the Milestone BoneSTATION for fixing and decalcifying bone tissue: http://www.milestonemedsrl.com/histopathology/products/pre-analytical-tools/bonestation.html Is it good? Not good? Easy to operate and maintain? Do you use it with in-house decalcifying solutions or commercial available ones? Have you tried their MOL-Decal solution on BMT? Any thoughts? What about the optimization and standardization of protocols? Is it quite straight forward? Or complicated / Difficult? We are considering it and any feedback would be much appreciated. Send me a PM if you like. Many Thanks, Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 133, Issue 16 ***************************************** From klaus.dern44 <@t> gmail.com Tue Dec 16 08:01:39 2014 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Tue Dec 16 08:01:46 2014 Subject: [Histonet] THICK AND THIN SECTIONS ? Message-ID: If you are using one of the following Microtomes and the advance mechanism is worn out. ( too much play between spindle and spindlenut ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome because these models are no longer supported by the manufacturer. ( No parts availability ) Rather than replacing these excellent instruments, I have a PERMANENT solution to fix this problem. For Information contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com From oneilb <@t> wvuhealthcare.com Tue Dec 16 08:36:22 2014 From: oneilb <@t> wvuhealthcare.com (O'neil, Beth) Date: Tue Dec 16 08:36:29 2014 Subject: [Histonet] Toluidine blue background Message-ID: <3CEB8EBCF9C7A648B9694B5696462A7140B3A190@NT-EX2.wvuhs.com> We would like to use Toluidine Blue for staining frozen sections of kidney biopsy. We finally are able to get the stain dark enough, but we can't get rid of the background caused by the OCT. We've washed with water, alcohols, and PBS with no success. Is anyone out there using Toluidine Blue for frozen staining? If so, what is your recipe and how do you get rid of the background? Thanks Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC oneilb@wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From JWatson <@t> gnf.org Tue Dec 16 09:00:24 2014 From: JWatson <@t> gnf.org (James Watson) Date: Tue Dec 16 09:00:46 2014 Subject: [Histonet] THICK AND THIN SECTIONS ? In-Reply-To: References: Message-ID: This is at least the third time I have seen this message. Is this a form of advertisement on the Histonet? Grumpy in San Diego because it is supposed to rain today. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Klaus Dern [klaus.dern44@gmail.com] Sent: Tuesday, December 16, 2014 6:01 AM To: histonet Subject: [Histonet] THICK AND THIN SECTIONS ? If you are using one of the following Microtomes and the advance mechanism is worn out. ( too much play between spindle and spindlenut ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome because these models are no longer supported by the manufacturer. ( No parts availability ) Rather than replacing these excellent instruments, I have a PERMANENT solution to fix this problem. For Information contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ryan.Roy <@t> va.gov Tue Dec 16 09:51:34 2014 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Tue Dec 16 09:51:55 2014 Subject: [Histonet] THICK AND THIN SECTIONS ? In-Reply-To: References: Message-ID: <15F883394EAB744E99E1C7E1B987304901770400214A@R04BYNMSGB1.r04.med.va.gov> Probably, Also, just because a manufacturer doesn't sell a part doesn't mean you can't get a part somewhere else. Ryan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, December 16, 2014 10:00 AM To: Klaus Dern; histonet Subject: [EXTERNAL] RE: [Histonet] THICK AND THIN SECTIONS ? This is at least the third time I have seen this message. Is this a form of advertisement on the Histonet? Grumpy in San Diego because it is supposed to rain today. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Klaus Dern [klaus.dern44@gmail.com] Sent: Tuesday, December 16, 2014 6:01 AM To: histonet Subject: [Histonet] THICK AND THIN SECTIONS ? If you are using one of the following Microtomes and the advance mechanism is worn out. ( too much play between spindle and spindlenut ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome because these models are no longer supported by the manufacturer. ( No parts availability ) Rather than replacing these excellent instruments, I have a PERMANENT solution to fix this problem. For Information contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flnails <@t> texaschildrens.org Tue Dec 16 09:55:27 2014 From: flnails <@t> texaschildrens.org (Nails, Felton) Date: Tue Dec 16 09:55:34 2014 Subject: [Histonet] CPT Code Changes Message-ID: <327E034F1892504289B7A17EC71DF9F308E2600C@TCFMSG03.ad.texaschildrenshospital.org> Was just wondering how everyone is handling the new changes to IHC 88342 and the use of 88341? Thanks. ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From TanyaAbbott <@t> catholichealth.net Tue Dec 16 11:08:11 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Tue Dec 16 11:10:47 2014 Subject: [Histonet] Pennsylvania Department of Health permit Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CD86070@CHIEX005.CHI.catholichealth.net> This is for you Histoneters In Pennsylvania! Are you all familiar with PA Department of Health Act 122-2013? I am wondering how the Pennsylvanians are interpreting the rules for sending out slides for consults to other states? Are you expecting those consulting labs to have PA DOH permits? Some of our consulting labs perform further testing that we cannot perform here in our lab, and some perform further testing that we can perform here, to aid in their consult opinion. And sometimes they just look at our material and give an opinion. Any ideas? Thanks in advance and happy holidays!! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From SThompson2 <@t> ecommunity.com Tue Dec 16 11:19:34 2014 From: SThompson2 <@t> ecommunity.com (Thompson, Samantha J) Date: Tue Dec 16 11:19:41 2014 Subject: [Histonet] RE: Toluidine blue background In-Reply-To: <3CEB8EBCF9C7A648B9694B5696462A7140B3A190@NT-EX2.wvuhs.com> References: <3CEB8EBCF9C7A648B9694B5696462A7140B3A190@NT-EX2.wvuhs.com> Message-ID: <5898BCD87BCDCF48A4AB066A91B477D5C95228@sdcexud2a.chi.ecommunity.com> We use T blue for frozen sections that we make using Toludine Blue O powder (ITEM SPT1182) from American Master Tech. Disolve 2.5 g of the powder into 500 mL DI H2O. I warm the water to help the powder completely dissolve and continually stir the until solution is made. I usually make up in a gallon size container and just pour into coplin jar for use. I fix the frozen sections in 100% ETOH ~30sec and rinse well in running tap water ~15 - 30 secs. Stain in T-Blue for 3 minutes and then set the stain with a borax solution* for ~ 15 secs. Rinse in running tap water 10 sec. and airdry. Clear with xylene or substitute and coverslip. I never have any background staining and no issues with remaining OCT. The T-blue lasts for about a month depending on how many slides you are running through it. I use the borax for two days before dumping and refilling. *borax solution made....100mL borax + 3000mL DI H2O. Again I make this up in a gallon size to pour as needed I hope this helps! Samantha Thompson Pathology Manager Dawes Fretzin Dermatology Group ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of O'neil, Beth [oneilb@wvuhealthcare.com] Sent: Tuesday, December 16, 2014 9:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue background We would like to use Toluidine Blue for staining frozen sections of kidney biopsy. We finally are able to get the stain dark enough, but we can't get rid of the background caused by the OCT. We've washed with water, alcohols, and PBS with no success. Is anyone out there using Toluidine Blue for frozen staining? If so, what is your recipe and how do you get rid of the background? Thanks Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC oneilb@wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans <@t> histologistics.com Tue Dec 16 12:09:01 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Tue Dec 16 12:09:11 2014 Subject: [Histonet] THICK AND THIN SECTIONS ? In-Reply-To: <15F883394EAB744E99E1C7E1B987304901770400214A@R04BYNMSGB1.r04.med.va.gov> References: <15F883394EAB744E99E1C7E1B987304901770400214A@R04BYNMSGB1.r04.med.va.gov> Message-ID: We have a Leica 2125 that I have been servicing for 2+ years and have had no problems at all. I think anyone with an engineering background could do it. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Tue, Dec 16, 2014 at 10:51 AM, Roy, Ryan wrote: > > Probably, > > Also, just because a manufacturer doesn't sell a part doesn't mean you > can't get a part somewhere else. > > Ryan > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson > Sent: Tuesday, December 16, 2014 10:00 AM > To: Klaus Dern; histonet > Subject: [EXTERNAL] RE: [Histonet] THICK AND THIN SECTIONS ? > > This is at least the third time I have seen this message. Is this a form > of advertisement on the Histonet? > > Grumpy in San Diego because it is supposed to rain today. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of Klaus Dern [ > klaus.dern44@gmail.com] > Sent: Tuesday, December 16, 2014 6:01 AM > To: histonet > Subject: [Histonet] THICK AND THIN SECTIONS ? > > If you are using one of the following Microtomes and the advance mechanism > is worn out. > ( too much play between spindle and spindlenut ) > > REICHERT/JUNG 2030 > LEICA RM 2125 > LEICA 2030 Biocut > LEICA/JUNG 2035 > LEICA - CM 1850 Cryostat > SAKURA SRM 200 > > You could be faced with purchasing a new Microtome because these models > are no longer supported by the manufacturer. ( No parts availability ) > > Rather than replacing these excellent instruments, I have a PERMANENT > solution to fix this problem. > > For Information contact: > > Klaus Dern > Phone: 706 635-8840 > E-Mail: klaus.dern44@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Richard.Cartun <@t> hhchealth.org Tue Dec 16 13:27:28 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Tue Dec 16 13:27:47 2014 Subject: [Histonet] Coxsackie virus testing Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355F25A0@HHCEXCHMB03.hhcsystem.org> Is anyone doing Coxsackie virus detection on formalin-fixed, paraffin-embedded tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From WaitT <@t> livemail.uthscsa.edu Tue Dec 16 16:54:13 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Tue Dec 16 16:54:21 2014 Subject: [Histonet] TRAP Stain Troubleshooting Message-ID: <1418770453609.52786@livemail.uthscsa.edu> Hello Histonetters! I have a few questions regarding TRAP Staining and I'm hoping someone who has experience with this stain could help me out. I have been using a protocol that is apparently not goo because it hasn't shown any TRAP whatsoever. I have seen various themes in several sources, one of those is that slides are incubated for 1 hour in pH 9.4 TBS before being put into the TRAP staining solution. My question for this is why? In my protocol, I have a TRAP incubation medium that contains the tartaric acid, that is at pH of 5.0, which I then combine with Naphthol and Fast Red (which are the reagents responsible for the chromogenicity of the stain). I guess I'm confused as to what the tartaric acid is used for as well....which I'm speculating that it denatures most of the enzymes other than TRAP (hence the name Tartrate Resistant Acid Phosphatase). Therefore, I see the three main steps for this protocol to be: 1. Incubate in TBS pH 9.4 (which I'm still confused why) 2. Combine with Tartaric Acid to denature all of the other enzymes to localize osteoclasts 3. Add Naphthol and Fast Red to Identify where TRAP is. Therefore, I'm confused why I am combining Tartaric Acid with my Napthol and Fast Red....is this a common method that is done to eliminate multiple unnecessary steps? If somebody has a protocol that works consistently for them and wouldn't mind sharing that with me, I would very appreciative of you! I'm just trying to understand this stain so that I can somehow tweak it to work. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate From bszpunar <@t> umail.iu.edu Wed Dec 17 18:49:44 2014 From: bszpunar <@t> umail.iu.edu (Bryan Szpunar) Date: Wed Dec 17 18:49:49 2014 Subject: [Histonet] Re: New H&E stainer validation Message-ID: Hi Albert, I would think of this more in terms of "performance verification" than validation, where the instrument is concerned. You verify that the instrument is working and you also validate the staining protocols you run on it. Performance verification can include several different things, such as electrical checks, having training documentation in place, and any installation qualification done by the installer/vendor/etc. For an automated stainer, running several test slides to ensure the mechanisms are operating correctly would also be appropriate. And of course, document, document, document. Basically, you should perform some kind of documented verification that the instrument performs properly and as expected, then address the specific staining protocols as a second "piece" of the puzzle (although you likely will perform both functions concurrently). For an H&E, I would usually want to see a minimum of 20 samples stained, reflecting a good mix of the types of samples you expect to see--guessing in your case mostly Derm stuff. Specifics for the validation, though, are mostly the purview of your medical director. Your question was fairly general, so hopefully I've focused on things that are helpful to you. Regards, Bryan Szpunar, HT(ASCP)CM From richard.wild <@t> wanadoo.fr Thu Dec 18 00:34:04 2014 From: richard.wild <@t> wanadoo.fr (richard wild) Date: Thu Dec 18 00:34:09 2014 Subject: [Histonet] Parts for Sakura Tissue-Tek GLC 550 glass coverslipper Message-ID: <5492755C.3030302@wanadoo.fr> Hello all, Sakura (Tissue-Tek) GLC 550 glass coverslipper and CR 10030 DAKO coverslipper are almost the same. But the slide baskets are not compatible because of some small differences. I have both. I am using mostly sakura stainers. So, I need to transform the CR 10030 DAKO coverslipper to accept the Sakura baskets - it is possible : _ __I am looking for some small "used" parts of a Sakura (Tissue-Tek) GLC 550 glass coverslipper._ Can someone help ? Another solution would be to find_adapters for Sakura stainer type DRS2000 or DRS601 to use the DAKO slide basket_ (the one that fits CR 10030 DAKO coverslipper) - I dont know it these exist. Do you know about these adapters ? Do you have any ? Best regards Richard From j.rowaihi <@t> alborglaboratories.com Thu Dec 18 01:08:30 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Thu Dec 18 01:08:52 2014 Subject: [Histonet] CONFIRM anti-Melanosome (HMB45) Mouse Monoclonal Primary Antibody, Catalog Number 790-4366 Message-ID: <039401d01a91$6fa5eeb0$4ef1cc10$@rowaihi@alborglaboratories.com> Dear colleagues I am not ok with staining of Melanosome (HMB45) Ventana using BenchMark GX The expiry due date still valid and I follow the staining protocol from the pamphlet but no good stain show even on the melanoma control sections. The protocol is : Recommended Staining Protocol for CONFIRM anti-Melanosome (HMB45) with ultraView Universal Red on BenchMark XT. CONFIRM anti-Melanosome (HMB45) Mouse Monoclonal Primary Antibody, Catalog Number 790-4366 Procedure Type Method BenchMark XT Deparaffinization Selected Cell Conditioning (Antigen Unmasking) None required Enzyme (Protease) None required Antibody (Primary) Approximately 8 minutes, 37 ?C Counterstain Hematoxylin II, 4 minutes Post Counterstain Bluing Reagent, 4 minutes Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From tfountain <@t> dsmanitoba.ca Thu Dec 18 08:12:53 2014 From: tfountain <@t> dsmanitoba.ca (Tiana Baskin) Date: Thu Dec 18 08:13:28 2014 Subject: [Histonet] p16 [INK4] clone G175-405 Message-ID: Hi Histonetters, I am trying to work up the Biogenex p16 on FFPE tissue and I am not having much luck. Does anyone have any experience with this antibody and getting it to work. I have tried a wide range of dilutions and epitope retrieval (both heat and enzyme). Any suggestions? Thanks in advance :) Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From gordon <@t> winlabscientific.com Thu Dec 18 16:00:08 2014 From: gordon <@t> winlabscientific.com (Gordon Wingate) Date: Fri Dec 19 10:35:58 2014 Subject: [Histonet] slide racks & carriers Message-ID: Hello Richard A friend in the US sent the following extract from histonet to me . We supply many racks and carriers for autostaining machines including Sakura , Dako etc Please go to our website for more information Kind Regards Gordon Wingate Operations Director Winlab Pty Ltd, 5/2 Pinnacle St, Brendale, Queensland, Australia Postal Address: PO Box 5007, Brendale, Queensland, 4500 Australia Ph: 61 7 3205 5233 Fax: 61 7 3205 1209 gordonw@winlab.com.au http://www.winlab.com.au We like to close the loop and manufacture purely our own developments. Design it, Make it and Sell it, that's what we do best, its best for us, and its best for our Customers. Histology - Cytology - Microbiology - Haematology Staining Racks - Carriers - Handles & Filter Funnels Hello all, Sakura (Tissue-Tek) GLC 550 glass coverslipper and CR 10030 DAKO coverslipper are almost the same. But the slide baskets are not compatible because of some small differences. I have both. I am using mostly sakura stainers. So, I need to transform the CR 10030 DAKO coverslipper to accept the Sakura baskets - it is possible : _ __I am looking for some small "used" parts of a Sakura (Tissue-Tek) GLC 550 glass coverslipper._ Can someone help ? Another solution would be to find_adapters for Sakura stainer type DRS2000 or DRS601 to use the DAKO slide basket_ (the one that fits CR 10030 DAKO coverslipper) - I dont know it these exist. From lindamargraf <@t> gmail.com Fri Dec 19 13:49:17 2014 From: lindamargraf <@t> gmail.com (Linda Margraf) Date: Fri Dec 19 13:49:32 2014 Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta Message-ID: > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone From mtitford <@t> aol.com Fri Dec 19 14:15:50 2014 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Dec 19 14:15:57 2014 Subject: [Histonet] Thank you Dr Margraf! Message-ID: <8D1E9DFB772DC91-B24-62975@webmail-va031.sysops.aol.com> Thank you Dr Margraf, Dr Cope and now Dr Sengupta for giving up your time and hosting the "Histonet". It is an ideal way for histotechnologists and others to be kept informed. And Merry Christmas to everyone from the heart of Dixie! Michael Titford Pathology USA Mobile AL USA From Joyce.Weems <@t> emoryhealthcare.org Fri Dec 19 14:21:43 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Dec 19 14:22:01 2014 Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta In-Reply-To: References: Message-ID: It is hard to believe it has been that long! My goodness!! Thank you, Dr. Cope and Dr. Sengupta for this valuable resource you provide us. It is beyond measure! Happy holidays to everyone! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, December 19, 2014 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From PAMarcum <@t> uams.edu Fri Dec 19 14:23:11 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Dec 19 14:23:18 2014 Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta In-Reply-To: References: Message-ID: Thank you and all who help you for this service. It is greatly appreciated and needed for all who are in the Histology field. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, December 19, 2014 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Bonnie.Whitaker <@t> osumc.edu Fri Dec 19 14:39:16 2014 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Fri Dec 19 14:39:44 2014 Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta In-Reply-To: References: Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E0BC518C7@EXMBOX-VP05.OSUMC.EDU> I want to add my thanks to everyone, as well. This is a service that many of us rely on, and appreciate on a daily basis, even if we forget to voice it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Friday, December 19, 2014 3:23 PM To: 'Linda Margraf'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta Thank you and all who help you for this service. It is greatly appreciated and needed for all who are in the Histology field. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, December 19, 2014 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Fri Dec 19 15:19:13 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Fri Dec 19 15:19:20 2014 Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta In-Reply-To: <6B106EE8C8AAEF449DEA97921DEC11670E0BC518C7@EXMBOX-VP05.OSUMC.EDU> References: <6B106EE8C8AAEF449DEA97921DEC11670E0BC518C7@EXMBOX-VP05.OSUMC.EDU> Message-ID: <009001d01bd1$714904f0$53db0ed0$@caplab.org> For those around long enough to remember, when Histonet was first created, there was a contest to see who could come up with a catchy phrase to but on buttons to be worn at the NSH that year. The phrase was "Have we met on the Histonet?" and the author was yours truly. I think I may have the button around somewhere still. Many thanks for this valuable resource!! Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow / CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Whitaker, Bonnie Sent: Friday, December 19, 2014 3:39 PM To: 'Marcum, Pamela A'; 'Linda Margraf'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta I want to add my thanks to everyone, as well. This is a service that many of us rely on, and appreciate on a daily basis, even if we forget to voice it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Friday, December 19, 2014 3:23 PM To: 'Linda Margraf'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta Thank you and all who help you for this service. It is greatly appreciated and needed for all who are in the Histology field. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, December 19, 2014 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2015.0.5577 / Virus Database: 4253/8762 - Release Date: 12/18/14 From mjones <@t> metropath.com Fri Dec 19 15:25:09 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Fri Dec 19 15:25:25 2014 Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta In-Reply-To: References: Message-ID: Yes, this list/blog is greatly appreciated!! I am very humbled by what others are accomplishing in their histo ?world? and it?s nice to know that we share common issues that challenge our daily work and our brains!! (If I still have one, sometimes I?m not so sure :) Thank you and Merry Christmas to all on the Histonet!! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 12/19/14, 1:23 PM, "Marcum, Pamela A" wrote: >Thank you and all who help you for this service. It is greatly >appreciated and needed for all who are in the Histology field. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda >Margraf >Sent: Friday, December 19, 2014 1:49 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > >> Dear Histonetters, >> In a couple of weeks, Histonet will celebrate its 19th anniversary! It >>is hard to believe it has been in existence that long. I would like to >>say thanks to all the members who contribute to the list, sharing their >>time and expertise with others in histology-related fields. We currently >>have >3800 subscribers from at least 30 countries ( last time I >>counted). It is amazing to see how the list continues to provide new >>insights and information after all these years. >> I'd also like to say welcome to Dr Anita Sengupta, who is taking over >>for Dr Sandy Cope as a co-administrator of the list with me. Anita is >>also a pediatric pathologist and she will help with some of the issues >>with list subscribing etc. Please continue to be patient with us if you >>are having list issues as we often get busy in our "day jobs". If your >>message to the list doesn't post and it says it is because you are not a >>member, please see if you need to update your email address on the >>membership page. Remember you can only post to the list from an email >>address that matches exactly with one on the subscriber list. You can >>always send us an email at the histonet-owners address and we will try >>to help you out. Best wishes for a happy and safe holiday season. >> Linda M >> Histonet administrator >> >> Sent from my iPhone >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >---------------------------------------------------------------------- >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dolores_Fischer <@t> baxter.com Fri Dec 19 16:06:05 2014 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Fri Dec 19 16:06:11 2014 Subject: [Histonet] unsubscibe Message-ID: <7CA324454CDDCA40A56FC3C51923C0E44B770031@BN1PRD9201MB008.026d.mgd.msft.net> The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From Dolores_Fischer <@t> baxter.com Fri Dec 19 16:28:03 2014 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Fri Dec 19 16:28:08 2014 Subject: [Histonet] unsubscribe Message-ID: <7CA324454CDDCA40A56FC3C51923C0E44B77006E@BN1PRD9201MB008.026d.mgd.msft.net> The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From rmhickey88 <@t> gmail.com Sat Dec 20 09:36:51 2014 From: rmhickey88 <@t> gmail.com (Ryan Michael Hickey) Date: Sat Dec 20 09:36:55 2014 Subject: [Histonet] Request Recommendation for Fixative/Stains Article Message-ID: Hello All, Would anyone be willing to recommend any good articles on: 1. The historical use of fixatives 2. Fixatives and/or stains derived from natural sources (e.g., wine, honey, orcein, hematoxylin) I once saw a really great article on the topic, but am having trouble locating it. Thank you, Ryan Hickey, BS, HTL(ASCP)QIHC From marilyn.tyler <@t> uct.ac.za Sun Dec 21 03:09:21 2014 From: marilyn.tyler <@t> uct.ac.za (Marilyn Tyler) Date: Sun Dec 21 03:09:41 2014 Subject: [Histonet] unsubscribe Message-ID: <83CDE72B16A37A459F15D5A179C2543B33615DCF@SRVWINEXC001.wf.uct.ac.za> Marilyn Tyler UCT Dept Surgery J50/30 Old Main Building Groote Schuur Hospital Observatory Cape Town S.A. 021-4066227 ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. From jfray80 <@t> hotmail.com Sun Dec 21 19:08:50 2014 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Sun Dec 21 19:08:56 2014 Subject: [Histonet] Histology Position Message-ID: Abaxis Veterinarian Reference Lab, in Olathe , Kansas, is looking for a Histology Technician . This position requires good Embedding and Microtomy skills . Hours would be 3:00 am to 11:30 am or 4:00 am to 12:30 pm Monday through Friday . If your are interested in this position please send your resume to josephfrazee@abaxis.com . Only serious inquiries . From Joyce.Weems <@t> emoryhealthcare.org Sun Dec 21 19:29:37 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Sun Dec 21 19:30:57 2014 Subject: [Histonet] RE: unsubscribe In-Reply-To: <83CDE72B16A37A459F15D5A179C2543B33615DCF@SRVWINEXC001.wf.uct.ac.za> References: <83CDE72B16A37A459F15D5A179C2543B33615DCF@SRVWINEXC001.wf.uct.ac.za> Message-ID: Hi Marilyn, You must go to the website and do that. Just follow the instructions.. http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best wishes, Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn Tyler Sent: Sunday, December 21, 2014 4:09 AM To: histonet Subject: [Histonet] unsubscribe Marilyn Tyler UCT Dept Surgery J50/30 Old Main Building Groote Schuur Hospital Observatory Cape Town S.A. 021-4066227 ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Linda.Margraf <@t> cookchildrens.org Mon Dec 22 08:25:20 2014 From: Linda.Margraf <@t> cookchildrens.org (Linda Margraf) Date: Mon Dec 22 08:25:28 2014 Subject: [Histonet] RE: Histonet unsubscribing Message-ID: <928719B9EBFA1C4686918B975FF84528EE83EADA@CCHCSMBX04.CCHCS.LDAP> Hi Marilyn (and other Histonet subscribers): Marilyn, I removed you from the list. I do want to remind everyone they can remove themselves, subscribe, change to the digest or real-time version of the list etc. at the list website: http://lists.utsouthwestern.edu/mailman/listinfo/histonet It is usually easier and faster if you do these things yourself but Anita and I can help when you need us. Linda Margraf Histonet administrator -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, December 21, 2014 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 133, Issue 22 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. unsubscribe (Marilyn Tyler) ---------------------------------------------------------------------- Message: 1 Date: Sun, 21 Dec 2014 09:09:21 +0000 From: Marilyn Tyler Subject: [Histonet] unsubscribe To: histonet Message-ID: <83CDE72B16A37A459F15D5A179C2543B33615DCF@SRVWINEXC001.wf.uct.ac.za> Content-Type: text/plain; charset=WINDOWS-1252 Marilyn Tyler UCT Dept Surgery J50/30 Old Main Building Groote Schuur Hospital Observatory Cape Town S.A. 021-4066227 ________________________________ UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 133, Issue 22 ***************************************** From TanyaAbbott <@t> catholichealth.net Mon Dec 22 09:49:09 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Dec 22 09:51:01 2014 Subject: [Histonet] RE: Histonet Digest, Vol 133, Issue 21 In-Reply-To: <201412201802.sBKHuuAw010223@chippi001.catholichealth.net> References: <201412201802.sBKHuuAw010223@chippi001.catholichealth.net> Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CD88A5A@CHIEX005.CHI.catholichealth.net> I have been in my new role as a Pathology manager for a little over a year now and I don't know what I would have done without Histonet! It is a fabulous resource, and everyone's' silliness and lightheartedness makes me laugh!! Thank you everyone for all of your help, I'll continue to pick your brains in the new year!! Happy Holidays!!! Tanya G. Abbott Histology/Cytology St. Joseph Medical Center 610-378-2635 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, December 20, 2014 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 133, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Histonet anniversary and welcome to Dr Sengupta (Linda Margraf) 2. Thank you Dr Margraf! (mtitford@aol.com) 3. RE: Histonet anniversary and welcome to Dr Sengupta (Weems, Joyce K.) 4. RE: Histonet anniversary and welcome to Dr Sengupta (Marcum, Pamela A) 5. RE: Histonet anniversary and welcome to Dr Sengupta (Whitaker, Bonnie) 6. RE: Histonet anniversary and welcome to Dr Sengupta (Douglas Porter) 7. Re: Histonet anniversary and welcome to Dr Sengupta (Michael Ann Jones) 8. unsubscibe (Fischer, Dolores) 9. unsubscribe (Fischer, Dolores) 10. Request Recommendation for Fixative/Stains Article (Ryan Michael Hickey) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Dec 2014 13:49:17 -0600 From: Linda Margraf Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone ------------------------------ Message: 2 Date: Fri, 19 Dec 2014 15:15:50 -0500 From: mtitford@aol.com Subject: [Histonet] Thank you Dr Margraf! To: histonet@lists.utsouthwestern.edu Message-ID: <8D1E9DFB772DC91-B24-62975@webmail-va031.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Thank you Dr Margraf, Dr Cope and now Dr Sengupta for giving up your time and hosting the "Histonet". It is an ideal way for histotechnologists and others to be kept informed. And Merry Christmas to everyone from the heart of Dixie! Michael Titford Pathology USA Mobile AL USA ------------------------------ Message: 3 Date: Fri, 19 Dec 2014 20:21:43 +0000 From: "Weems, Joyce K." Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta To: "'Linda Margraf'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" It is hard to believe it has been that long! My goodness!! Thank you, Dr. Cope and Dr. Sengupta for this valuable resource you provide us. It is beyond measure! Happy holidays to everyone! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, December 19, 2014 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 4 Date: Fri, 19 Dec 2014 20:23:11 +0000 From: "Marcum, Pamela A" Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta To: "'Linda Margraf'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Thank you and all who help you for this service. It is greatly appreciated and needed for all who are in the Histology field. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, December 19, 2014 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 5 Date: Fri, 19 Dec 2014 15:39:16 -0500 From: "Whitaker, Bonnie" Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta To: "'Marcum, Pamela A'" , "'Linda Margraf'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E0BC518C7@EXMBOX-VP05.OSUMC.EDU> Content-Type: text/plain; charset=us-ascii I want to add my thanks to everyone, as well. This is a service that many of us rely on, and appreciate on a daily basis, even if we forget to voice it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Friday, December 19, 2014 3:23 PM To: 'Linda Margraf'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta Thank you and all who help you for this service. It is greatly appreciated and needed for all who are in the Histology field. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, December 19, 2014 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= ------------------------------ Message: 6 Date: Fri, 19 Dec 2014 16:19:13 -0500 From: "Douglas Porter" Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta To: "'Linda Margraf'" , Message-ID: <009001d01bd1$714904f0$53db0ed0$@caplab.org> Content-Type: text/plain; charset="iso-8859-1" For those around long enough to remember, when Histonet was first created, there was a contest to see who could come up with a catchy phrase to but on buttons to be worn at the NSH that year. The phrase was "Have we met on the Histonet?" and the author was yours truly. I think I may have the button around somewhere still. Many thanks for this valuable resource!! Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow / CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Whitaker, Bonnie Sent: Friday, December 19, 2014 3:39 PM To: 'Marcum, Pamela A'; 'Linda Margraf'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta I want to add my thanks to everyone, as well. This is a service that many of us rely on, and appreciate on a daily basis, even if we forget to voice it! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Friday, December 19, 2014 3:23 PM To: 'Linda Margraf'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histonet anniversary and welcome to Dr Sengupta Thank you and all who help you for this service. It is greatly appreciated and needed for all who are in the Histology field. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Friday, December 19, 2014 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > Dear Histonetters, > In a couple of weeks, Histonet will celebrate its 19th anniversary! It > is hard to believe it has been in existence that long. I would like to say thanks to all the members who contribute to the list, sharing their time and expertise with others in histology-related fields. We currently have >3800 subscribers from at least 30 countries ( last time I counted). It is amazing to see how the list continues to provide new insights and information after all these years. > I'd also like to say welcome to Dr Anita Sengupta, who is taking over > for Dr Sandy Cope as a co-administrator of the list with me. Anita is also a pediatric pathologist and she will help with some of the issues with list subscribing etc. Please continue to be patient with us if you are having list issues as we often get busy in our "day jobs". If your message to the list doesn't post and it says it is because you are not a member, please see if you need to update your email address on the membership page. Remember you can only post to the list from an email address that matches exactly with one on the subscriber list. You can always send us an email at the histonet-owners address and we will try to help you out. Best wishes for a happy and safe holiday season. > Linda M > Histonet administrator > > Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2015.0.5577 / Virus Database: 4253/8762 - Release Date: 12/18/14 ------------------------------ Message: 7 Date: Fri, 19 Dec 2014 21:25:09 +0000 From: Michael Ann Jones Subject: Re: [Histonet] Histonet anniversary and welcome to Dr Sengupta To: "Marcum, Pamela A" , 'Linda Margraf' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Yes, this list/blog is greatly appreciated!! I am very humbled by what others are accomplishing in their histo ?world? and it?s nice to know that we share common issues that challenge our daily work and our brains!! (If I still have one, sometimes I?m not so sure :) Thank you and Merry Christmas to all on the Histonet!! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 12/19/14, 1:23 PM, "Marcum, Pamela A" wrote: >Thank you and all who help you for this service. It is greatly >appreciated and needed for all who are in the Histology field. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda >Margraf >Sent: Friday, December 19, 2014 1:49 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Histonet anniversary and welcome to Dr Sengupta > >> Dear Histonetters, >> In a couple of weeks, Histonet will celebrate its 19th anniversary! It >>is hard to believe it has been in existence that long. I would like to >>say thanks to all the members who contribute to the list, sharing their >>time and expertise with others in histology-related fields. We currently >>have >3800 subscribers from at least 30 countries ( last time I >>counted). It is amazing to see how the list continues to provide new >>insights and information after all these years. >> I'd also like to say welcome to Dr Anita Sengupta, who is taking over >>for Dr Sandy Cope as a co-administrator of the list with me. Anita is >>also a pediatric pathologist and she will help with some of the issues >>with list subscribing etc. Please continue to be patient with us if you >>are having list issues as we often get busy in our "day jobs". If your >>message to the list doesn't post and it says it is because you are not a >>member, please see if you need to update your email address on the >>membership page. Remember you can only post to the list from an email >>address that matches exactly with one on the subscriber list. You can >>always send us an email at the histonet-owners address and we will try >>to help you out. Best wishes for a happy and safe holiday season. >> Linda M >> Histonet administrator >> >> Sent from my iPhone >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= > >---------------------------------------------------------------------- >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= ------------------------------ Message: 8 Date: Fri, 19 Dec 2014 22:06:05 +0000 From: "Fischer, Dolores" Subject: [Histonet] unsubscibe To: "histonet@lists.utsouthwestern.edu" Message-ID: <7CA324454CDDCA40A56FC3C51923C0E44B770031@BN1PRD9201MB008.026d.mgd.msft.net> Content-Type: text/plain; charset="us-ascii" The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.baxter.com_email-5Fdisclaimer&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=87mt2eehPvuxV53wNuX0yfUds3UEwK4231iPM-q79QM&e= ------------------------------ Message: 9 Date: Fri, 19 Dec 2014 22:28:03 +0000 From: "Fischer, Dolores" Subject: [Histonet] unsubscribe To: "histonet@lists.utsouthwestern.edu" Message-ID: <7CA324454CDDCA40A56FC3C51923C0E44B77006E@BN1PRD9201MB008.026d.mgd.msft.net> Content-Type: text/plain; charset="us-ascii" The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.baxter.com_email-5Fdisclaimer&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=87mt2eehPvuxV53wNuX0yfUds3UEwK4231iPM-q79QM&e= ------------------------------ Message: 10 Date: Sat, 20 Dec 2014 10:36:51 -0500 From: Ryan Michael Hickey Subject: [Histonet] Request Recommendation for Fixative/Stains Article To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hello All, Would anyone be willing to recommend any good articles on: 1. The historical use of fixatives 2. Fixatives and/or stains derived from natural sources (e.g., wine, honey, orcein, hematoxylin) I once saw a really great article on the topic, but am having trouble locating it. Thank you, Ryan Hickey, BS, HTL(ASCP)QIHC ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwID-g&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=g4FBz7vvGtRLUeySSg5in9VO3bYW2Zwtr23VlkDSsrg&s=wcqWdFxViihxo2jIOwkE-nT9C_jd3jcO4TcTtdCm-nA&e= End of Histonet Digest, Vol 133, Issue 21 ***************************************** This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From mtitford <@t> aol.com Mon Dec 22 14:27:03 2014 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Mon Dec 22 14:27:11 2014 Subject: [Histonet] Early fixatives Message-ID: <8D1EC3CC82B162C-1D04-6BF27@webmail-vd021.sysops.aol.com> Ryan Hickey asks about the historical use of fixatives, and stains derived from natural sources. A good place to start is with the book by Brian Bracegirdle " A History of Microtechnique" (second edition) Published by Scientific Heritage 1987. There are many articles out there and you will have to do a literature search for them. Regards Michael Titford USA Pathology Mobile AL USA From cjohnson <@t> nmda.nmsu.edu Mon Dec 22 15:00:05 2014 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Mon Dec 22 15:00:23 2014 Subject: [Histonet] Control strength? Message-ID: Hello all, How are you documenting trending signal strength of your controls for special stains and IHC? I was asked this question during an audit and would appreciate your help. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.838.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From rjbuesa <@t> yahoo.com Mon Dec 22 15:50:42 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 22 15:50:46 2014 Subject: [Histonet] Control strength? In-Reply-To: References: Message-ID: <1182123476.222917.1419285042280.JavaMail.yahoo@jws10095.mail.ne1.yahoo.com> As I see it, control "strength" is less important than its ADEQUACY for diagnosis.Every time a pathologists signs a case requiring a special procedure WITH a control, it means that the control was adequate, UNLESS the pathologist complains about it.Ren? J.? On Monday, December 22, 2014 4:00 PM, "Johnson, Carole" wrote: Hello all, How are you documenting trending signal strength of your controls for special stains and IHC? I was asked this question during an audit and would appreciate your help. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.838.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Dec 22 16:38:50 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Dec 22 16:38:57 2014 Subject: [Histonet] Control strength? - personal opinion Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDE9E@SBS2K8.premierlab.local> Rene I'm going to have to respectfully disagree with you. In my opinion this is the one of biggest problems we have now a days when we interpret our positive controls in histology. We have been determining if staining is acceptable verses not acceptable for years as "yes it stained no it did not stain" and we really have to change how we look at our controls over time. Staining intensity and consistency is key in determining overall quality of both special and IHC staining, especially when it comes to therapeutic markers and especially if we are utilizing image analysis for those markers like ER/PR and HER2. We need to look at what we do over time and get away from what we have been doing in the past, such as acceptable verses not acceptable. What we need to be able to define is the following especially in IHC staining - accuracy, precision, and robustness of staining just to name a few. It may be sometimes too late when a pathologist complains about it, that could mean that signal strength has been decreasing over time and has finally gotten to the point where the staining is inadequate. I would think we would want to address trending problems sooner, since weaker staining intensity in control tissue could lead to false negatives in samples that do not express the protein we are trying to detect at lower expression levels. This is what we do here. We scan all of our positive IHC control slides that we utilize for image analysis and track staining intensity overtime either by visually looking at the positive control slides (many at a time) or running image analysis and then graphing our staining intensity in a Levy Jennings chart. This is new for us and we are still working out the details on how we can interpret the data but we have found the information to be very valuable and enlightening. Just my two cents for whatever it is worth. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, December 22, 2014 2:51 PM To: Johnson, Carole; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Control strength? As I see it, control "strength" is less important than its ADEQUACY for diagnosis.Every time a pathologists signs a case requiring a special procedure WITH a control, it means that the control was adequate, UNLESS the pathologist complains about it.Ren? J.? On Monday, December 22, 2014 4:00 PM, "Johnson, Carole" wrote: Hello all, How are you documenting trending signal strength of your controls for special stains and IHC? I was asked this question during an audit and would appreciate your help. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.838.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Mon Dec 22 17:39:31 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Mon Dec 22 17:39:41 2014 Subject: [Histonet] Warthin Starry on Dako Artisan Message-ID: Happy Holidays Histonetters! Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate? With offline uranyl nitrate pretreatment, spirochetes stain brilliantly! But everything else we've tried leaves them invisible . . . Any guidance would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From rjbuesa <@t> yahoo.com Tue Dec 23 10:17:34 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 23 10:17:44 2014 Subject: [Histonet] Warthin Starry on Dako Artisan In-Reply-To: References: Message-ID: <2135595184.404708.1419351454650.JavaMail.yahoo@jws100202.mail.ne1.yahoo.com> Try a 0.10% solution of phosphotungstic acid.Ren? J.? On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" wrote: Happy Holidays Histonetters! Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate?? With offline uranyl nitrate pretreatment, spirochetes stain brilliantly!? But everything else we've tried leaves them invisible . . . Any guidance would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357? ? Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo <@t> pathlab.us Tue Dec 23 11:55:43 2014 From: histo <@t> pathlab.us (Histology) Date: Tue Dec 23 11:58:41 2014 Subject: [Histonet] Leder stain Message-ID: <566E7795BC94204D9A21539DE282AED725C141EE@DC01.dp.local> Hi All, Does anyone know where I can find the reagents to do the Leder stain (Naphthol AS-D Chloroacetate Esterase)? We usually get a kit from Sigma but they have been out since September. Thanks in advance, Mehndi Helgren Dominion Pathology Labs From annigyg <@t> gmail.com Tue Dec 23 13:01:14 2014 From: annigyg <@t> gmail.com (Anne Van Binsbergen) Date: Tue Dec 23 13:01:16 2014 Subject: [Histonet] Dako Artisan Warthin Starry Message-ID: <41A92BE5-6E73-4D67-AD82-02C81BA8004A@gmail.com> We do them successfully all the time. Without pretreatment. AnnieinArabia Sent from my iPhone From Ronie.Rodrigo <@t> advocatehealth.com Tue Dec 23 15:52:09 2014 From: Ronie.Rodrigo <@t> advocatehealth.com (Rodrigo, Ronie) Date: Tue Dec 23 15:52:17 2014 Subject: [Histonet] microarray Message-ID: Hello, I would like to know where I can buy a microarray tissue slides/blocks for IHC controls? Thank you. Ron Rodrigo ACL Histology Supervisor Rosemont, IL This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. From kdwyatt <@t> vet.k-state.edu Tue Dec 23 17:11:23 2014 From: kdwyatt <@t> vet.k-state.edu (Kristina Wyatt) Date: Tue Dec 23 17:11:28 2014 Subject: [Histonet] Research Assistant in Histology/Immunohistochemistry Position Message-ID: <1419376282573.66800@vet.k-state.edu> Kansas State Veterinary Diagnostic Laboratory - Research Assistant The Kansas State Veterinary Diagnostic Laboratory is hiring a full-time research assistant for the Histopathology/Immunohistochemistry laboratory. This person will perform histology and general laboratory duties related to service and research. Duties will include trimming (grossing) of fixed biopsy and necropsy tissues, cutting tissues on a microtome and preparing glass slides of the tissues in accordance with those QA/QC guidelines of an AAVLD accredited laboratory. Also, routine filing of slides, tissue blocks, data entry, operating and maintaining automated histology equipment, immunohistochemical staining, and operation and organization of digital pathology projects will be required. Significant attention must be given to the KSVDL QMS (Quality Management System) and subsequent compliance. A Bachelor of Science degree in biology or related field along with a minimum of six months of laboratory experience is required. Please send your letter of interest, resume or curriculum vitae, and contact information for three professional references to: Aubrey Baldwin, aubreyb85@vet.k-state.edu Screening of applicants will begin on January 12, 2015 and will continue until the position is filled. KSU is an equal opportunity employer of individuals with disabilities and protected veterans. Background check is required. From suetp918 <@t> comcast.net Tue Dec 23 18:25:53 2014 From: suetp918 <@t> comcast.net (Sue) Date: Tue Dec 23 18:26:20 2014 Subject: [Histonet] Leder stain In-Reply-To: <566E7795BC94204D9A21539DE282AED725C141EE@DC01.dp.local> References: <566E7795BC94204D9A21539DE282AED725C141EE@DC01.dp.local> Message-ID: <121871621.1402370.1419380753490.JavaMail.zimbra@comcast.net> good luck, we no longer do the stain but we only were able to get it from Sigma. From bcooper <@t> chla.usc.edu Tue Dec 23 18:35:52 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Dec 23 18:35:57 2014 Subject: [Histonet] RE: Leder stain In-Reply-To: <566E7795BC94204D9A21539DE282AED725C141EE@DC01.dp.local> References: <566E7795BC94204D9A21539DE282AED725C141EE@DC01.dp.local> Message-ID: Never done the stain myself, but have you tried Poly Scientific? Check out this link . . . http://www.polyrnd.com/products/reagent-assembly-kits/conventional/leder-stain-kit.aspx Thanks, Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology Sent: Tuesday, December 23, 2014 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leder stain Hi All, Does anyone know where I can find the reagents to do the Leder stain (Naphthol AS-D Chloroacetate Esterase)? We usually get a kit from Sigma but they have been out since September. Thanks in advance, Mehndi Helgren Dominion Pathology Labs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From ihcman2010 <@t> hotmail.com Wed Dec 24 05:05:53 2014 From: ihcman2010 <@t> hotmail.com (Glen) Date: Wed Dec 24 05:06:22 2014 Subject: [Histonet] from: Glen Message-ID: <154EE849-B6E4-45F3-EB83-CF4F27BB6946@al.senai.br> Hi histonet http://voluntaryaction-er.org.uk/object.php?science=fda62vvv0qg4 ihcman2010@hotmail.com Sent from my iPhone From jqb7 <@t> cdc.gov Wed Dec 24 07:13:22 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Dec 24 07:13:46 2014 Subject: [Histonet] Warthin Starry on Dako Artisan In-Reply-To: <2135595184.404708.1419351454650.JavaMail.yahoo@jws100202.mail.ne1.yahoo.com> References: <2135595184.404708.1419351454650.JavaMail.yahoo@jws100202.mail.ne1.yahoo.com> Message-ID: <3B2CD438E1628A41BD687E98B963B7812B926B15@EMBX-CLFT4.cdc.gov> What protocol with uranyl nitrate do you use? Dako told us to use zinc formalin as a sensitizer but it doesn't seem to make much of a difference. Thanks! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 23, 2014 11:18 AM To: Cooper, Brian; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry on Dako Artisan Try a 0.10% solution of phosphotungstic acid.Ren? J.? On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" wrote: Happy Holidays Histonetters! Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate?? With offline uranyl nitrate pretreatment, spirochetes stain brilliantly!? But everything else we've tried leaves them invisible . . . Any guidance would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357? ? Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Dec 24 07:20:10 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Dec 24 07:20:32 2014 Subject: [Histonet] Warthin Starry on Dako Artisan In-Reply-To: <3B2CD438E1628A41BD687E98B963B7812B926B15@EMBX-CLFT4.cdc.gov> References: <2135595184.404708.1419351454650.JavaMail.yahoo@jws100202.mail.ne1.yahoo.com> <3B2CD438E1628A41BD687E98B963B7812B926B15@EMBX-CLFT4.cdc.gov> Message-ID: <3B2CD438E1628A41BD687E98B963B7812B926B42@EMBX-CLFT4.cdc.gov> Also, what thickness do you cut your sections? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, December 24, 2014 8:13 AM To: 'Cooper, Brian'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Warthin Starry on Dako Artisan What protocol with uranyl nitrate do you use? Dako told us to use zinc formalin as a sensitizer but it doesn't seem to make much of a difference. Thanks! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 23, 2014 11:18 AM To: Cooper, Brian; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry on Dako Artisan Try a 0.10% solution of phosphotungstic acid.Ren? J.? On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" wrote: Happy Holidays Histonetters! Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate?? With offline uranyl nitrate pretreatment, spirochetes stain brilliantly!? But everything else we've tried leaves them invisible . . . Any guidance would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357? ? Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From crawford <@t> arrayscience.com Wed Dec 24 14:39:18 2014 From: crawford <@t> arrayscience.com (Scott Crawford) Date: Wed Dec 24 14:39:23 2014 Subject: [Histonet] Re: microarray [for IHC controls, etc] Message-ID: Dear Ron, and histonet community, We at Array Science offer tissue microarrays in block or slide form for IHC controls. We have substantial experience in the design and production of TMAs that cover multiple markers in relatively concise configurations. Please contact us at info_at_arrayscience.com. Scott Crawford, PhD Array Science, LLC Tissue Microarray Technologies crawford_at_arrayscience.com > Hello, I would like to know where I can buy a microarray tissue slides/blocks for IHC controls? > > Thank you. > Ron Rodrigo > ACL Histology Supervisor > Rosemont, IL From bcooper <@t> chla.usc.edu Wed Dec 24 17:30:07 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Wed Dec 24 17:30:21 2014 Subject: [Histonet] Warthin Starry on Dako Artisan In-Reply-To: <3B2CD438E1628A41BD687E98B963B7812B926B15@EMBX-CLFT4.cdc.gov> References: <2135595184.404708.1419351454650.JavaMail.yahoo@jws100202.mail.ne1.yahoo.com> <3B2CD438E1628A41BD687E98B963B7812B926B15@EMBX-CLFT4.cdc.gov> Message-ID: Hi, We've had really good results with pretreatment in 1% Uranyl Nitrate, as well as with 1% Uranyl Acetate in Acetate Buffer--both at room temperature, and at 60 degrees C for 10 minutes. We've also tried zinc formalin, as well as "Spirochete Sensitizer"--a commercially available product, but we've only obtained extremely faint staining of spirochetes with the latter two. Our sections are cut at 4 ?m. Thanks, Brian -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:jqb7@cdc.gov] Sent: Wednesday, December 24, 2014 5:13 AM To: Cooper, Brian; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Warthin Starry on Dako Artisan What protocol with uranyl nitrate do you use? Dako told us to use zinc formalin as a sensitizer but it doesn't seem to make much of a difference. Thanks! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, December 23, 2014 11:18 AM To: Cooper, Brian; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Warthin Starry on Dako Artisan Try a 0.10% solution of phosphotungstic acid.Ren? J.? On Monday, December 22, 2014 6:39 PM, "Cooper, Brian" wrote: Happy Holidays Histonetters! Has anyone out there successfully stained spirochetes with Dako's Artisan Warthin-Starry kit, without first pretreating the sections with uranyl nitrate?? With offline uranyl nitrate pretreatment, spirochetes stain brilliantly!? But everything else we've tried leaves them invisible . . . Any guidance would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357? ? Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From alineumann <@t> aol.com Fri Dec 26 10:32:34 2014 From: alineumann <@t> aol.com (Alice Neumann) Date: Fri Dec 26 10:32:39 2014 Subject: [Histonet] Practical Reviews in Pathology CDs Message-ID: <8D1EF40AFE9A0C9-2284-744D0@webmail-m288.sysops.aol.com> I have about a years worth of these. ?Does anyone want them, esp. people in resource limited countries? ? Alice Neumann MD Pinnacle Pathology PC 207 Auburn Dr. ?#1 Auburn, AL 36830 24 Hour Cell Phone:? 307-413-4092 alineumann@aol.com ? From crawford <@t> arrayscience.com Sat Dec 27 16:33:29 2014 From: crawford <@t> arrayscience.com (Scott Crawford) Date: Sat Dec 27 16:33:37 2014 Subject: [Histonet] Histotechs Wanted, full or part-time, Sausalito, CA In-Reply-To: References: Message-ID: <116ee6909b6b11b5f5b87ecf2763a1eb@arrayscience.com> Histotech Positions Available Immediately Sausalito, CA Full or Part-time. Flexible hours, including daytime. Excellent cutting skills required. Must have good references. Contact: info@arrayscience.com --- Scott Crawford, PhD Chief Technical Officer Array Science, LLC Tissue Microarray Technologies crawford@arrayscience.com --- From info <@t> biopathx.com Sun Dec 28 14:58:04 2014 From: info <@t> biopathx.com (BioPathx) Date: Sun Dec 28 14:58:18 2014 Subject: [Histonet] RE: Controls Message-ID: <1066526937.4194244.1419800284800.open-xchange@bosoxweb01.eigbox.net> You can find CMV and many other positive control slides in Biopathx.com. Biopathx offers the best price and some free control slides with your first order. You may contact info@biopathx.com for detail. From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen Sent: Friday, December 12, 2014 7:16 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls Help! Running low on CMV and AFB positive control blocks. Looking into buying precut slides but the price is steep. Any suggestion ? Which company has the best price. ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. _____________________________________________________________________________________________ From THicks <@t> gulfcoastdermpath.com Mon Dec 29 03:49:23 2014 From: THicks <@t> gulfcoastdermpath.com (Trish Hicks) Date: Mon Dec 29 03:49:31 2014 Subject: [Histonet] Position in Port Charlotte/Punta Gorda, Florida Message-ID: <96D42D0E9FB88A46A1B3E3E82DE4CA9A1F46ADE3@GCD-EXCH.tampa.gcd.local> I am posting this position for a friend. A large Dermatology practice in beautiful Port Charlotte and Punta Gorda is seeking a Florida Licensed, part-time histotech for Routine Histology (technical component portion) and MOHS. 20-30/wk Competitive salary and a friendly staff Send Resume to: PO Box 494710, Port Charlotte, FL 33949 OR Fax resume to: 941-613-2401 Attention: Tracy Rohling, Office Manager Thank You, Trish Hicks Confidentiality: The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of this information by persons or entities other than the intended recipient(s) is unauthorized and prohibited. Any transmission of confidential and/or privileged material to persons or entities other than the intended recipient(s) shall not be construed as a waiver of any privilege or confidence. If you receive this transmission in error, please contact the sender and delete the material. Virus Disclaimer: The contents of an attachment to this e-mail may contain software viruses, which could damage your computer system. We cannot accept liability for any damage you may sustain as a result of software viruses. You should carry out your own virus checks before opening any attachment. From Luis.Chiriboga <@t> nyumc.org Mon Dec 29 07:31:27 2014 From: Luis.Chiriboga <@t> nyumc.org (Chiriboga, Luis) Date: Mon Dec 29 07:31:39 2014 Subject: [Histonet] Job Opening Message-ID: <3E6798F00C9F494399E96B720ECD142936D518@MSGWCDCPMB22.nyumc.org> A position is available for a full-time histotech in a busy, exciting NYC research Histopathology Core. The Core provides state-of-the-art histology services, training and consulting for the scientific and clinical research community. We seek a dynamic, motivated individual with expertise in histology and related techniques. The primary responsibilities will include tissue specimen processing, embedding, sectioning, and staining; automated and manual immunohistochemical and immunofluorescence techniques. Preparation of reagents and solutions required for analysis. Maintain laboratory instrumentation, equipment, supply and inventory. Perform quality control and quality assurance tasks as required. Strong communication and language skills are critical as the position requires active interactions with the Core's customers, including one-on-one training, and assisting in the collection and organization of data and results. Competency with standard office software such as MS-Word and Excel and Lab Information Management Systems (LIMS) is expected. The position requires a BS degree or higher and at least 2 years lab experience utilizing histology-related techniques. Qualified candidates please send resume, cover letter detailing prior histology experience, and three references to andrea.ohlsson@nyumc.org Luis Chiriboga Ph.D, Director OCS Experimental Pathology IHC Core Lab NYU School of Medicine Smilow 308/310 Office: 646-501-6934 Mobile: (347) 712-0897 Luis.Chiriboga@nyumc.org From nguy0515 <@t> gmail.com Mon Dec 29 11:29:06 2014 From: nguy0515 <@t> gmail.com (Trini Nguyen) Date: Mon Dec 29 11:29:10 2014 Subject: [Histonet] Sakura DRS 2000 stainer protocol Message-ID: Hello histonetters! Anyone currently using a Sakura DRS 2000 stainer thats running derm specimens only who would be willing to share your protocol? It would be greatly appreciated!! From tejohnson <@t> genoptix.com Mon Dec 29 12:09:19 2014 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Mon Dec 29 12:09:29 2014 Subject: [Histonet] QC daily H&Es Message-ID: What are the CLIA/CAP labs doing for daily H&E QC? Does a pathologist need to review the H&E control slide daily, or do you have an ASCP certified tech delegated to doing this? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From joelleweaver <@t> hotmail.com Mon Dec 29 13:50:29 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Mon Dec 29 13:50:33 2014 Subject: [Histonet] QC daily H&Es In-Reply-To: References: Message-ID: Both. The pathologist has to sign off. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tejohnson@genoptix.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 29 Dec 2014 18:09:19 +0000 > Subject: [Histonet] QC daily H&Es > > What are the CLIA/CAP labs doing for daily H&E QC? Does a pathologist need to review the H&E control slide daily, or do you have an ASCP certified tech delegated to doing this? > > Teri Johnson, HT(ASCP)QIHC > Manager Clinical Trial Testing > Genoptix, Inc. > SAN5, Rm. 2005 > 760.516.5954 (office) > 760.516.6201 (fax) > > > ________________________________ > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Mon Dec 29 15:47:21 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Dec 29 15:47:27 2014 Subject: [Histonet] PML - JC virus Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355FCA2F@HHCEXCHMB03.hhcsystem.org> Can anyone recommend a good antibody (for IHC testing) to identify "JC" virus (Polyomavirus) in the brain (PML)? I finally ran out of my polyclonal anti-SV40 antibody that I have been using for the past 25 years. Best wishes to everyone for the coming New Year! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From jburch01 <@t> gmail.com Mon Dec 29 16:31:41 2014 From: jburch01 <@t> gmail.com (Jim Burchette) Date: Mon Dec 29 16:31:45 2014 Subject: [Histonet] PML - JC virus In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355FCA2F@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E355FCA2F@HHCEXCHMB03.hhcsystem.org> Message-ID: Richard, I always used Oncogenes SV40 antibody for PML. On Dec 29, 2014 3:47 PM, "Cartun, Richard" wrote: > Can anyone recommend a good antibody (for IHC testing) to identify "JC" > virus (Polyomavirus) in the brain (PML)? I finally ran out of my > polyclonal anti-SV40 antibody that I have been using for the past 25 > years. Best wishes to everyone for the coming New Year! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pwnoyce <@t> gmail.com Mon Dec 29 18:27:07 2014 From: pwnoyce <@t> gmail.com (Peter Noyce) Date: Mon Dec 29 18:26:15 2014 Subject: [Histonet] formaldehyde concentration Message-ID: <000101d023c7$5a443d30$0eccb790$@gmail.com> I am using pure formaldehyde with no methanol and unbuffered as a first fixative. It comes in concentrations from 20% to 4%. From my reading; 1)it seems the higher strength penetrates faster ?. 2)4% is the minimum strength to get good penetration ?. Peter Noyce PhD student Australia. From mchilds2010 <@t> hotmail.com Mon Dec 29 18:39:10 2014 From: mchilds2010 <@t> hotmail.com (Hotmail) Date: Mon Dec 29 18:39:23 2014 Subject: [Histonet] 2015 Florida Society Meeting CALL FOR ABSTRACTS Message-ID: Dear Friends and Colleagues, I am happy to announce the official call for abstract submission for the 2015 Florida Society meeting. All abstracts should be submitted by January 31, 2015. The meeting will be held at the Buena Vista Palace Hotel and Spa in Orlando May 15-17, 2015. We will also be continuing the Administrators and Supervisors Academy this year and are seeking abstracts for the academy as well. Please submit to John Shelley, President, at jshelley@sanfordburnham.org And/or Michelle Foster, Vice President and Meeting Planner, at mchilds2010@hotmail.com Thank you, Michelle Foster Sent from my iPhone From JMacDonald <@t> mtsac.edu Tue Dec 30 01:51:52 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Dec 30 01:51:58 2014 Subject: [Histonet] CSH 2015 Message-ID: We are looking for workshops for our 2015 meeting. Does anyone have a MOHS workshop that they would like to present? A workshop on grossing? Thanks, Jennifer MacDonald CSH Education Chair From CDavis <@t> che-east.org Tue Dec 30 07:19:54 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Tue Dec 30 07:24:51 2014 Subject: [Histonet] A sticky subject.... Message-ID: Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From PAMarcum <@t> uams.edu Tue Dec 30 07:45:10 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Dec 30 07:45:21 2014 Subject: [Histonet] RE: A sticky subject.... In-Reply-To: References: Message-ID: I am not sure where you live so this may or may not apply. I have lived in several placed and one I learned no matter where I am when they turn on the heat and you wear anything with polyester or that can become electrostatic we had issues with static. I am not sure how to fit it as I have made sure I touch something metal to discharge the static. It does not cure the problem only humidity will and being careful not to start to cut fully charged so to say. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, December 30, 2014 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A sticky subject.... Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From foreightl <@t> gmail.com Tue Dec 30 07:53:01 2014 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Dec 30 07:53:06 2014 Subject: [Histonet] RE: A sticky subject.... In-Reply-To: References: Message-ID: I used to have one of those anti-static brushes, it had a small radioactive piece of metal that discharged the static. It worked sometimes. Try putting moistened gauze, or the kimwipes used to clean your waterbath in your microtome wax waste tray. That seems to help me. Good luck. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A wrote: > I am not sure where you live so this may or may not apply. I have lived > in several placed and one I learned no matter where I am when they turn on > the heat and you wear anything with polyester or that can become > electrostatic we had issues with static. I am not sure how to fit it as I > have made sure I touch something metal to discharge the static. It does > not cure the problem only humidity will and being careful not to start to > cut fully charged so to say. > > Pam Marcum > UAMS > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > Sent: Tuesday, December 30, 2014 7:20 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] A sticky subject.... > > Good morning Histo folks, > > I am hoping somebody can help me. Today was a horrid microtomy > day, the static was brutal. I tried putting lotion on my hands, the fabric > softener sheet, and wearing gloves but to no avail. I heard rumors somebody > used to manufactor an anti-static brush but have yet to find one. Does > anybody have any other tricks that could be shared? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria <@t> gmail.com Tue Dec 30 07:58:21 2014 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Tue Dec 30 07:58:35 2014 Subject: [Histonet] RE: A sticky subject.... In-Reply-To: References: Message-ID: Before I start cutting I spray StaticGard on the arms of my lab coat. I don't know what lotion you use but a non lanolin based moisturizer helped me (corn huskers lotion - it is glycerin based). This works for some but it comes down to trying different things to see what will work for you. Vikki On Dec 30, 2014 8:53 AM, "Patrick Laurie" wrote: > I used to have one of those anti-static brushes, it had a small radioactive > piece of metal that discharged the static. It worked sometimes. Try > putting moistened gauze, or the kimwipes used to clean your waterbath in > your microtome wax waste tray. That seems to help me. > > Good luck. > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > > On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A > wrote: > > > I am not sure where you live so this may or may not apply. I have lived > > in several placed and one I learned no matter where I am when they turn > on > > the heat and you wear anything with polyester or that can become > > electrostatic we had issues with static. I am not sure how to fit it as > I > > have made sure I touch something metal to discharge the static. It does > > not cure the problem only humidity will and being careful not to start to > > cut fully charged so to say. > > > > Pam Marcum > > UAMS > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > > Sent: Tuesday, December 30, 2014 7:20 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] A sticky subject.... > > > > Good morning Histo folks, > > > > I am hoping somebody can help me. Today was a horrid microtomy > > day, the static was brutal. I tried putting lotion on my hands, the > fabric > > softener sheet, and wearing gloves but to no avail. I heard rumors > somebody > > used to manufactor an anti-static brush but have yet to find one. Does > > anybody have any other tricks that could be shared? > > > > Cassandra Davis > > CDavis@che-east.org > > 302-575-8095 > > > > > > > > Confidentiality Notice: > > This e-mail, including any attachments is the property of Trinity Health > > and is intended for the sole use of the intended recipient(s). It may > > contain information that is privileged and confidential. Any > unauthorized > > review, use, disclosure, or distribution is prohibited. If you are not > the > > intended recipient, please delete this message, and reply to the sender > > regarding the error in a separate email. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ---------------------------------------------------------------------- > > Confidentiality Notice: This e-mail message, including any attachments, > is > > for the sole use of the intended recipient(s) and may contain > confidential > > and privileged information. Any unauthorized review, use, disclosure or > > distribution is prohibited. If you are not the intended recipient, please > > contact the sender by reply e-mail and destroy all copies of the original > > message. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mpence <@t> grhs.net Tue Dec 30 07:58:21 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Dec 30 07:58:40 2014 Subject: [Histonet] RE: A sticky subject.... In-Reply-To: References: Message-ID: Try Google for anti-static brush. There are several listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, December 30, 2014 7:54 AM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; Davis, Cassie Subject: Re: [Histonet] RE: A sticky subject.... I used to have one of those anti-static brushes, it had a small radioactive piece of metal that discharged the static. It worked sometimes. Try putting moistened gauze, or the kimwipes used to clean your waterbath in your microtome wax waste tray. That seems to help me. Good luck. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A wrote: > I am not sure where you live so this may or may not apply. I have > lived in several placed and one I learned no matter where I am when > they turn on the heat and you wear anything with polyester or that can > become electrostatic we had issues with static. I am not sure how to > fit it as I have made sure I touch something metal to discharge the > static. It does not cure the problem only humidity will and being > careful not to start to cut fully charged so to say. > > Pam Marcum > UAMS > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > Sent: Tuesday, December 30, 2014 7:20 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] A sticky subject.... > > Good morning Histo folks, > > I am hoping somebody can help me. Today was a horrid > microtomy day, the static was brutal. I tried putting lotion on my > hands, the fabric softener sheet, and wearing gloves but to no avail. > I heard rumors somebody used to manufactor an anti-static brush but > have yet to find one. Does anybody have any other tricks that could be shared? > > Cassandra Davis > CDavis@che-east.org > 302-575-8095 > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity > Health and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis <@t> che-east.org Tue Dec 30 09:10:14 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Tue Dec 30 09:10:31 2014 Subject: [Histonet] Sticky subject responses...Thank you Message-ID: To all you wonderful folks who responded, a big thanks! I've been doing histo since 1990 and NEVER had static like today, it's not usually that big of a problem in this mid-Atlantic state. When it does occur the tricks I named earlier usually help but I am always up for learning something new. I will try the low tech option of moist something in my clean-up tray tomorrow, first. I usually can't stand anything in my tray after facing but if given a choice I'll suck it up. I will keep you posted. Thanks again! Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Timothy.Morken <@t> ucsf.edu Mon Dec 29 13:58:22 2014 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Dec 30 09:11:41 2014 Subject: [Histonet] RE: QC daily H&Es In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367CEFAB@ex07.net.ucsf.edu> Teri, our AM techs (ASCP) check the QC slide and sign off on it. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Monday, December 29, 2014 10:09 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] QC daily H&Es What are the CLIA/CAP labs doing for daily H&E QC? Does a pathologist need to review the H&E control slide daily, or do you have an ASCP certified tech delegated to doing this? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd <@t> chs.net Tue Dec 30 09:36:36 2014 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Tue Dec 30 09:37:40 2014 Subject: [Histonet] RE: A sticky subject.... In-Reply-To: References: , Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9EF97AC@TN001WEXMBX014.US.chs.net> A humidifier and a cool room temp does the job for us. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Victoria Baker [bakevictoria@gmail.com] Sent: Tuesday, December 30, 2014 8:58 AM To: Patrick Laurie Cc: histonet@lists.utsouthwestern.edu; Davis, Cassie; Marcum, Pamela A Subject: Re: [Histonet] RE: A sticky subject.... Before I start cutting I spray StaticGard on the arms of my lab coat. I don't know what lotion you use but a non lanolin based moisturizer helped me (corn huskers lotion - it is glycerin based). This works for some but it comes down to trying different things to see what will work for you. Vikki On Dec 30, 2014 8:53 AM, "Patrick Laurie" wrote: > I used to have one of those anti-static brushes, it had a small radioactive > piece of metal that discharged the static. It worked sometimes. Try > putting moistened gauze, or the kimwipes used to clean your waterbath in > your microtome wax waste tray. That seems to help me. > > Good luck. > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > > On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A > wrote: > > > I am not sure where you live so this may or may not apply. I have lived > > in several placed and one I learned no matter where I am when they turn > on > > the heat and you wear anything with polyester or that can become > > electrostatic we had issues with static. I am not sure how to fit it as > I > > have made sure I touch something metal to discharge the static. It does > > not cure the problem only humidity will and being careful not to start to > > cut fully charged so to say. > > > > Pam Marcum > > UAMS > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > > Sent: Tuesday, December 30, 2014 7:20 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] A sticky subject.... > > > > Good morning Histo folks, > > > > I am hoping somebody can help me. Today was a horrid microtomy > > day, the static was brutal. I tried putting lotion on my hands, the > fabric > > softener sheet, and wearing gloves but to no avail. I heard rumors > somebody > > used to manufactor an anti-static brush but have yet to find one. Does > > anybody have any other tricks that could be shared? > > > > Cassandra Davis > > CDavis@che-east.org > > 302-575-8095 > > > > > > > > Confidentiality Notice: > > This e-mail, including any attachments is the property of Trinity Health > > and is intended for the sole use of the intended recipient(s). It may > > contain information that is privileged and confidential. Any > unauthorized > > review, use, disclosure, or distribution is prohibited. If you are not > the > > intended recipient, please delete this message, and reply to the sender > > regarding the error in a separate email. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ---------------------------------------------------------------------- > > Confidentiality Notice: This e-mail message, including any attachments, > is > > for the sole use of the intended recipient(s) and may contain > confidential > > and privileged information. Any unauthorized review, use, disclosure or > > distribution is prohibited. If you are not the intended recipient, please > > contact the sender by reply e-mail and destroy all copies of the original > > message. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From turkekul <@t> gmail.com Tue Dec 30 12:48:08 2014 From: turkekul <@t> gmail.com (Mesru T) Date: Tue Dec 30 12:48:13 2014 Subject: [Histonet] Re: Histonet Digest, Vol 133, Issue 30 In-Reply-To: <54a2e82f.e242b60a.5b90.5ea8SMTPIN_ADDED_MISSING@mx.google.com> References: <54a2e82f.e242b60a.5b90.5ea8SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: I use Anti-Static Static-Away brush from Ted Pella Inc. Product no: 51922 5.7cm Handle length www.tedpella.com it works very well, I clean the metal surfaces of the paraffin microtome and do not wet the brush. Good Luck, Mesru From jaylundgren <@t> gmail.com Tue Dec 30 14:56:58 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Dec 30 14:57:06 2014 Subject: [Histonet] RE: A sticky subject.... In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246A9EF97AC@TN001WEXMBX014.US.chs.net> References: <7EAFE982E328304DA6CE2B677BB76246A9EF97AC@TN001WEXMBX014.US.chs.net> Message-ID: They sell water based anti static sprays for computer clean rooms and such. https://shop.eis-inc.com/sap(bD1lbiZjPTAwMQ==)/bc/bsp/sap/zeis2/index.htm?prod_area=371 They work great. Just be sure to spray the floor and the soles of your shoes, as well as the microtome and benchtop. Also, some microtomes have a grounding strap or a place to attach a grounding strap. Or you can wear a ground strap on your wrist, although this is a little restrictive. The best solution IMHO, ground your microtome and use professional anti-static spray (Not from the grocery store.) Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Dec 30, 2014 at 9:36 AM, Boyd, Debbie M wrote: > A humidifier and a cool room temp does the job for us. > > Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical > Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH > 804-765-5025 | FAX 804-765-6058 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] on behalf of Victoria Baker [ > bakevictoria@gmail.com] > Sent: Tuesday, December 30, 2014 8:58 AM > To: Patrick Laurie > Cc: histonet@lists.utsouthwestern.edu; Davis, Cassie; Marcum, Pamela A > Subject: Re: [Histonet] RE: A sticky subject.... > > Before I start cutting I spray StaticGard on the arms of my lab coat. I > don't know what lotion you use but a non lanolin based moisturizer helped > me (corn huskers lotion - it is glycerin based). This works for some but > it comes down to trying different things to see what will work for you. > Vikki > On Dec 30, 2014 8:53 AM, "Patrick Laurie" wrote: > > > I used to have one of those anti-static brushes, it had a small > radioactive > > piece of metal that discharged the static. It worked sometimes. Try > > putting moistened gauze, or the kimwipes used to clean your waterbath in > > your microtome wax waste tray. That seems to help me. > > > > Good luck. > > > > Patrick Laurie(HT)ASCP QIHC > > > > Histology Manager > > > > Celligent Diagnostics, LLC > > > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > > > Work: 704-970-3300 Cell: 704-266-0869 > > > > On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A > > wrote: > > > > > I am not sure where you live so this may or may not apply. I have > lived > > > in several placed and one I learned no matter where I am when they turn > > on > > > the heat and you wear anything with polyester or that can become > > > electrostatic we had issues with static. I am not sure how to fit it > as > > I > > > have made sure I touch something metal to discharge the static. It > does > > > not cure the problem only humidity will and being careful not to start > to > > > cut fully charged so to say. > > > > > > Pam Marcum > > > UAMS > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie > > > Sent: Tuesday, December 30, 2014 7:20 AM > > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] A sticky subject.... > > > > > > Good morning Histo folks, > > > > > > I am hoping somebody can help me. Today was a horrid microtomy > > > day, the static was brutal. I tried putting lotion on my hands, the > > fabric > > > softener sheet, and wearing gloves but to no avail. I heard rumors > > somebody > > > used to manufactor an anti-static brush but have yet to find one. Does > > > anybody have any other tricks that could be shared? > > > > > > Cassandra Davis > > > CDavis@che-east.org > > > 302-575-8095 > > > > > > > > > > > > Confidentiality Notice: > > > This e-mail, including any attachments is the property of Trinity > Health > > > and is intended for the sole use of the intended recipient(s). It may > > > contain information that is privileged and confidential. Any > > unauthorized > > > review, use, disclosure, or distribution is prohibited. If you are not > > the > > > intended recipient, please delete this message, and reply to the sender > > > regarding the error in a separate email. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ---------------------------------------------------------------------- > > > Confidentiality Notice: This e-mail message, including any attachments, > > is > > > for the sole use of the intended recipient(s) and may contain > > confidential > > > and privileged information. Any unauthorized review, use, disclosure or > > > distribution is prohibited. If you are not the intended recipient, > please > > > contact the sender by reply e-mail and destroy all copies of the > original > > > message. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -------------------------------------------------------------------------- > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pwnoyce <@t> gmail.com Tue Dec 30 16:23:35 2014 From: pwnoyce <@t> gmail.com (Peter Noyce) Date: Tue Dec 30 16:22:43 2014 Subject: [Histonet] formaldehyde concentration In-Reply-To: <2332a49269e04571aef02e9e3ea4c9b7@MAIL13M2N1.ad.uams.edu> References: <000101d023c7$5a443d30$0eccb790$@gmail.com> <2332a49269e04571aef02e9e3ea4c9b7@MAIL13M2N1.ad.uams.edu> Message-ID: <000f01d0247f$43c16a20$cb443e60$@gmail.com> Thank you I should give more details -I am not actually doing histology but trying to find a method to preserve tiny plant shoot apical meristems (approximately 0.05mm cubed in volume) so I can then measure the amount of light organic elements (CONPS) in a section by using x-ray dispersive spectroscopy with scanning electron microscopy. I thought a variant of Karnovskys fixative with no buffer may do it-mix equal amounts of formaldehyde (say 16%% pure with no methanol and not 10% buffered formalin) and glutaraldehyde (25% EM grade)-so the formaldehyde penetrates the tiny sample very quickly with fast initial fixation and then glutaraldehyde gets in more slowly but gives more extensive stronger fixation. Am I wrong-will the formaldehyde initially harden the sample so the glutaraldehyde may not get in ?. Should I use the lowest formaldehyde concentration that will give initial fixation ( say 8% which will end up as 4% when diluted with equal amounts of glut.). Please let me know what you think. Regards Peter -----Original Message----- From: Marcum, Pamela A [mailto:PAMarcum@uams.edu] Sent: Wednesday, 31 December 2014 12:51 AM To: 'Peter Noyce' Subject: RE: [Histonet] formaldehyde concentration Peter, While 20% sounds like it should be faster it is not. It hardens the outside of the tissue quickly and can slow penetration to the remainder of the tissue. We use 4% which if you start full strength liquid formaldehyde actually with 1 part formaldehyde and 9 parts buffer or water is 3.7% that we can 10% formalin. It is a real issue as we are basing the 10% number on the amount of formaldehyde (37 - 40%) added to buffer or water not the actual amounts. Sorry it is confusing about amounts. Let me know if you need more information or this sounds dodgy. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Noyce Sent: Monday, December 29, 2014 6:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formaldehyde concentration I am using pure formaldehyde with no methanol and unbuffered as a first fixative. It comes in concentrations from 20% to 4%. From my reading; 1)it seems the higher strength penetrates faster ?. 2)4% is the minimum strength to get good penetration ?. Peter Noyce PhD student Australia. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mprice63 <@t> live.com Tue Dec 30 17:31:59 2014 From: mprice63 <@t> live.com (Marsha Price) Date: Tue Dec 30 17:32:09 2014 Subject: [Histonet] 'Help' Message-ID: Sent from my iPhone From algranth <@t> email.arizona.edu Wed Dec 31 06:28:59 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Dec 31 06:29:06 2014 Subject: [Histonet] RE: A sticky subject.... In-Reply-To: References: Message-ID: Somebody has mentioned a humidifier and I found this to be one of the simplest solutions to the static problem. I got one at Target for a good price and used DI water in it because our tap water here is awful. My lab was in Tucson where we have little humidity anyway and the static often could have been very bad. I was cutting serial sections often and needed every possible section for things like 3D reconstruction, etc. I also put wet kimwipes in the waste tray of my microtome as well and discouraged other people from walking around in the area as to not stir up the airflow. I had some antistatic devices at one time and disposing of them became a problem when the powers that be discovered that the devices had a small amt of radioactivity. Geeze, you would have thought I was going to threaten to blow up the lab with dry, crystallized picric acid! As a side note, the plants in the lab loved the humidifier! Good luck. Andi Grantham Retired Histotech and loving it! ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Davis, Cassie [CDavis@che-east.org] Sent: Tuesday, December 30, 2014 6:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A sticky subject.... Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ecameron1 <@t> midcoasthealth.com Wed Dec 31 08:56:52 2014 From: ecameron1 <@t> midcoasthealth.com (Cameron, Elizabeth) Date: Wed Dec 31 08:56:58 2014 Subject: [Histonet] Giemsa - smears vs. paraffin sections Message-ID: Happy New Year, Histonetters! I have always been taught that a Wright-Giemsa stain was strictly for smears, and a different modification (i.e. May-Grunwald) should be used for tissues. I was recently asked what the reasoning was, and to be honest I am not sure. I understand that tissue stains differently from smears, and I would imagine timing would be different, but is there a reason the solutions are different? Very curious. Thanks! -Liz From ttruscot <@t> vetmed.wsu.edu Wed Dec 31 13:42:53 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Wed Dec 31 13:43:09 2014 Subject: [Histonet] thanks Message-ID: <9EF5279EBDFE6E4FB6605E8F183A0027D69C61C8@CVM76.vetmed.wsu.edu> Many thanks to all who contribute and keep the histonet running. I'm working my last day here at USDA-ARS ADRU within WSU which has been a very rewarding and enjoyable occupation. I was honored to be on 14 publications in 12 years with a few more in the works. Due to Federal budget constraints, they don't plan on replacing me with another BS An. Sci. ASCP HT. Happy New Year to you all! Thanks again Tom T= Thomas C. Truscott From christiecgowan <@t> dermatology.med.ufl.edu Tue Dec 30 14:48:51 2014 From: christiecgowan <@t> dermatology.med.ufl.edu (Gowan,Christie C) Date: Tue Jan 6 11:42:32 2015 Subject: [Histonet] Cost per block on the Sakura Xpress Message-ID: Dear Histonetters, Does anyone have an algorithm for cost per block on the Sakura Xpress? I would need it for biopsy specimens only. I inherited one of these machines and before I put it into use, I want to know what it will cost me and yes I have reached out to Sakura (Florida) but no responses as of yet. Many thanks! Christie Gowan HT (ASCP) University of Florida Department of Dermatology 4037 NW 86th Terrace Gainesville, FL 32606 Phone: 352 594-1529