[Histonet] Re cd4 1F6 clone

[Steven Weston]

Hi Histonetters
Has anyone else had difficulty getting  this clone to work? cd4 1F6 from leica microsystems (formerly novocastra).
I've tried just about every trick in my book and still no staining.
Particularly i made sure i did the peroxidase block prior to HIER (i even tried using it after the primary) and have tried low, high and middle pH buffers with antibody concentrations from 1/10-1/40 for 60 and 90 mins and use envision plus detection with DAB plus. I've had two batches of this over the last ten years and neither has worked.
Anyone else with the same experience ?
regards
steve weston
lab manager
Breathe-Well CRE
UTAS-SOM