From PREISZNE <@t> mail.etsu.edu Fri Aug 1 08:30:10 2014 From: PREISZNE <@t> mail.etsu.edu (Preiszner, Johanna) Date: Fri Aug 1 08:30:15 2014 Subject: [Histonet] Re: Helicobacter control Message-ID: Hi, I'd like to thank everybody for offering a control block! Again, it's fantastic to see how helpful people are here on the Histonet! Hanna Preiszner From MMargiotta <@t> bmhmc.org Fri Aug 1 10:44:09 2014 From: MMargiotta <@t> bmhmc.org (Margiotta-Watz, Michele) Date: Fri Aug 1 10:44:14 2014 Subject: [Histonet] unsubscribe Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC4277F081@BMH-EXCHANGE-02.BMHMC.ORG> Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From TJohnson <@t> gnf.org Fri Aug 1 12:10:25 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Fri Aug 1 12:10:31 2014 Subject: [Histonet] Re:unsubscribe Message-ID: <9F3CFEE76E51B64991C7485270890B40498FBE5D@EX5.lj.gnf.org> I got this, Peggy... To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From JJennings <@t> thedermlab.com Fri Aug 1 12:31:21 2014 From: JJennings <@t> thedermlab.com (Jonathan Jennings) Date: Fri Aug 1 12:31:50 2014 Subject: [Histonet] RE: Noise reduction In-Reply-To: References: , Message-ID: <000f4250.021316b07cdda76c@thedermlab.com> You might want your PA to try a quality foam microphone cover that fits your dictation device. Noice cancellation is what they are meant for. If it works it would be very inexpensive. Jonathan Jennings DermLab Pathology Manager This email was sent from my mobile device. Please excuse any typing errors ------ Original message------ From: Jay Lundgren Date: Thu, Jul 31, 2014 3:10 PM To: Tony Reilly; Cc: histonet@lists.utsouthwestern.edu; Subject:Re: [Histonet] RE: Noise reduction I don't know if it's your PA not being able to concentrate, or your transcriptionists complaining about noise on the recording, but have you considered solving the problem on the other end? Noise canceling headphones and/or a throat microphone for your PA. A real throat mic, (not a video game accessory) works by picking up vibrations of the throat with transducers, so you don't get background noise. Voila! No noise in your PA's ears, or on transcriptionist's recordings. And your histotechs can still party! Sincerely, Jay A. Lundgren, M.S., HTL, (ASCP) On Wed, Jul 30, 2014 at 5:25 PM, Tony Reilly wrote: > Hi LeAnn > > At a previous laboratory we had a half wall with glass from bench height > to the ceiling and a glass door. The door could be open or closed and the > glass while blocking out noise did not make the area feel confined from the > rest of the lab. The other advantages were that the air conditioning to > that room was independent and not recycled to the rest of the lab reducing > formalin fumes and if there was a formalin spill in that area it was easily > contained. > > Regards > Tony > > > Tony Reilly B.App.Sc, M.Sc > Chief Scientist Anatomical Pathology > Princess Alexandra Laboratory | Pathology Queensland | Health Support > Queensland > Department of Health | Queensland Government > Building 15, Level 1, > 199 Ipswich Road > Woolloongabba Qld 4102 > Ph: 07 3176 2412 > Mob: 0402139411 > Fax: 07 3176 2930 > Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang > Sent: Thursday, 31 July 2014 4:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Noise reduction > > We are trying to figure out an inexpensive way to try to block off our > grossing area to help with noise reduction when our Pathologist Assistant > does her dictation. Has anyone done anything in their labs that they would > be willing to share with me? We don't want something permanent like > building a wall, but rather something that can be opened up for parts of > the day. We have thought about having a curtain (like a privacy curtain > found in doctors' offices), but we are not sure that would be enough of a > reduction. Anyone have other ideas? > > Thanks much! > LeAnn > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > LeAnn Lang > Associates in Pathology > Practice Administrator > Phone: 715-847-0075 (ext 50259) > llang@aipathology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ******************************************************************************** > This email, including any attachments sent with it, is confidential and > for the sole use of the intended recipient(s). This confidentiality is not > waived or lost, if you receive it and you are not the intended > recipient(s), or if it is transmitted/received in error. > Any unauthorised use, alteration, disclosure, distribution or review of > this email is strictly prohibited. The information contained in this > email, including any attachment sent with it, may be subject to a statutory > duty of confidentiality if it relates to health service matters. > If you are not the intended recipient(s), or if you have received this > email in error, you are asked to immediately notify the sender by telephone > collect on Australia +61 1800 198 175 or by return email. You should > also delete this email, and any copies, from your computer system network > and destroy any hard copies produced. > If not an intended recipient of this email, you must not copy, distribute > or take any action(s) that relies on it; any form of disclosure, > modification, distribution and/or publication of this email is also > prohibited. > Although Queensland Health takes all reasonable steps to ensure this email > does not contain malicious software, Queensland Health does not accept > responsibility for the consequences if any person's computer inadvertently > suffers any disruption to services, loss of information, harm or is > infected with a virus, other malicious computer programme or code that may > occur as a consequence of receiving this email. > Unless stated otherwise, this email represents only the views of the > sender and not the views of the Queensland Government. > > ********************************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Fri Aug 1 13:26:03 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Aug 1 13:26:07 2014 Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 1 In-Reply-To: <20140801161158.042481E807D@trendmess-svr.holyredeemer.local> References: <20140801161158.042481E807D@trendmess-svr.holyredeemer.local> Message-ID: On the TV show, "Get Smart" they had the "cone of silence" Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 6. Re: RE: Noise reduction (Jay Lundgren) > We are trying to figure out an inexpensive way to try to block off our > grossing area to help with noise reduction when our Pathologist Assistant > does her dictation. --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From yhuang773 <@t> yahoo.com Fri Aug 1 13:52:11 2014 From: yhuang773 <@t> yahoo.com (Yan Huang) Date: Fri Aug 1 13:52:13 2014 Subject: [Histonet] =?iso-8859-1?q?=E8=87=AA=E5=8A=A8=E5=9B=9E=E5=A4=8D?= Message-ID: <908457.58099.bm@omp1011.mail.sg3.yahoo.com> I have changed my email to yhuang773@gmail.com and please contact me by the gmail address in the future. From deliacs <@t> uhnj.org Fri Aug 1 14:34:11 2014 From: deliacs <@t> uhnj.org (Delia, Catherine) Date: Fri Aug 1 14:35:39 2014 Subject: [Histonet] RE: unsubscribe In-Reply-To: <230D0B9EC57D7A45A7A186C6AB4C7ABC4277F081@BMH-EXCHANGE-02.BMHMC.ORG> References: <230D0B9EC57D7A45A7A186C6AB4C7ABC4277F081@BMH-EXCHANGE-02.BMHMC.ORG> Message-ID: <97BC9AFB15C7A5488E52257DB913EE189368B84C1F@UHEXMBX01.core.umdnj.edu> Catherine Susan Delia, BS. HT. ASCP Chief Technologist, Anatomic Pathology University Hospital 150 Bergen Street E-151 Newark, New Jersey 07103 Phone: 973-972-5717 Cell: 908-391-1060 Cell: 973-289-9123 Fax: 973-972-5724 deliacs@uhnj.org From craigak12 <@t> gmail.com Fri Aug 1 15:13:45 2014 From: craigak12 <@t> gmail.com (Jb) Date: Fri Aug 1 15:13:55 2014 Subject: [Histonet] Specimen transport container: Message-ID: <257AEB1F-DD73-4C1F-B7E3-85335FF74287@gmail.com> We are looking to transfer blocks in formalin for processing. Does anyone know of a good formalin/liquid tight container that would be good to use for transport? Thank you, Sent from my iPhone From gayle.callis <@t> bresnan.net Fri Aug 1 16:33:50 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Aug 1 16:34:06 2014 Subject: [Histonet] Re: Schiff's storage temperature - long reply Message-ID: <000601cfadd0$4ae57bf0$e0b073d0$@bresnan.net> Dear Histonetters, At the risk this may be more information than you need. My fascination with PAS and Schiffs reagents prompted the following. Our Schiffs reagent, prior to available commercial products, was made in house. We made Lillies Cold Schiffs solution stored at 4C according to protocol but no time limit was given. Another in house Barger and DeLamater reagent said several months at 4C - a rather vague time. Luna said 3 months storage at 4C. I think the kicker here is to test any Schiffs before use since it is unstable over time to remove any doubt. Other differences is basic fuchsin versus pararosaniline dyes and there are publications discussing these dyes for Schiffs reagents. Confusing - yes!! Back in the dark ages when practical exams were still done for HTL, curiosity led to testing both in house and a commercial Schiffs reagent. All staining was done at RT in parallel fashion using fresh in house and commercial Schiffs on adjacent sections/separate slides. We noted no visible staining difference i.e. intensity. This led exclusive use of commercial Schiffs to avoid last minute, tedious and exposure to carcinogenic basic fuchsin i.e. the weighing out dry, and strong HCl fumes. We didn't always have good fume hoods back in the dark ages of histology. Old habits die hard to store Schiffs at 4C, so RT storage was ignored, and 4C prevailed with commercial Schiffs with staining at RT. However, if I had to have immediate PAS staining in a busty lab, I would store Schiffs at RT to avoid waiting for equilibration to RT. This brings up a question. Has the person asking at temperature storage tried staining with cold versus warm as the staining times I saw i.e. up to 30 minutes could very well make up the difference. Cold Schiffs longer time versus warm Schiffs shorter time? 1% versus 0.5% periodic acid will also make a difference. The best discussion of Periodic acid Schiffs reaction staining mechanisms and technical comments can be found in Sheehan and Hrapchak Theory and Practice of Histotechnology, Carbohydrate Chapter 9, p9 164 166. Second Edition, 1980. This book is a classic and still available in Paperback form from NSH. This gives excellent hints on performing the stain i.e. 1) always used freshly prepared periodic acid (PA) something Culling insisted on. PA is cheap and goes into solution immediately. I do not use premade periodic acid solutions in kits as I don't consider these freshly made, simply unused but made days, weeks, months before purchasing the kit. If they are used, should be discarded and go to making PA fresh daily. Another finding was a decision to preweigh dry aliquouts of PA into a clear glass Erlenmeyer flasks to save time, seal the top of flask tightly, store on shelf until distilled water was added. This did NOT work as the preweighed PA went bad due to exposure to air, and probably light. PAS staining was poor, renal pathologist complained, and was embarrassing. Freshly weighed, solution prep daily was reinstated. Some things we did to maintain good PAS staining. 1. Since shelf life varies with use and probably storage conditions, expiration date is important but in house Schiffs could go bad before that. This is probably true of commercial solutions too. 1. If Schiffs reagent turns pink, discard. 3. Never freeze Schiffs reagent. Shipment to cold weather states i.e. Montana in winter was avoided after Schiffs arrived frozen. Plan ahead. 4. Never return used Schiffs to stock bottle. Store in another bottle, dated and tightly sealed. Expendable plastic wrap makes a good tight seal around and under lids on bottles or Coplin jars with used Schiffs. 6. Test used and even new if the latter hasn't been used for a time after opening stock bottle. Sheehan and Hrapchak have test in chapter. We just used a few drops of Schiffs in NBF and watch the bad color.......deep blue-purple. You want instant bright red with slight tinge of purple. If the lab is formalin free, use histologic test by staining cross section of human appendix, look for good staining of fine meshwork sarcolemma between smooth muscle cells. 7. Reuse Schiffs for up to a week, unless it turns pink. 8. Do not dump Schiffs down the drain, discard using appropriate chemical safety facility. 9. Don't get Schiffs on your skin.......nitrile gloves. Even though some vendors may have Schiffs removal reagents, once on the skin, you are already exposed to basic fuchsin - the contamination/exposure horse is already out of the barn. 10. Sulfurous acid rinses were discontinued after hearing Culling (in person) lecture on PAS staining. He said a 10 minute running tap water rinse was sufficient to intensify the color and we never had a problem using only water rinse. We have used Sigma's Schiffs, and also Fisher reagent grade Schiffs with great success. I think commercial solutions are wonderful, and you may find subtle differences in a given lab's storage, usage and conditions. If they work, then use them but be aware of limitations. If RT storage speeded up my time for results, then I would do just that. Otherwise, I would still store a 4C, equilibrate to RT. Remember there are people who use microwave heating for a PAS stain. PAS success also depends on what structures are being stained and thickness of sections. Our 2 ?m renal biopsy section protocol had different timing that staining 5 ?m sections from other organs. If anyone wants this chapter, I will be happy to scan to pdf and send via private email. Hope your weekend is a good one Gayle M. Callis HTL/HT/MT(ASCP) From Maxim_71 <@t> mail.ru Sat Aug 2 13:17:31 2014 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Aug 2 13:17:28 2014 Subject: [Histonet] Re: Schiff's storage temperature - long reply Message-ID: <199801356.20140802221731@mail.ru> Dear histonetters, I want to add, that it is very important to use Schiff's reagent in lab environment without any formaline vapours. -- Sincerely, Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru From craigak12 <@t> gmail.com Sun Aug 3 13:45:49 2014 From: craigak12 <@t> gmail.com (Jb) Date: Sun Aug 3 13:45:55 2014 Subject: [Histonet] IHC validation: Message-ID: <5E734B43-C215-46D7-9D1A-C03644D18BDB@gmail.com> Does anyone have experience validating antibodies using tissue microarrays? Is this a preferred method? I was thinking of punching different tissues/cases, then embedding them in one block, and then validating the antibody. Please advise. Thank you. Sent from my iPhone From suetp918 <@t> comcast.net Sun Aug 3 14:48:31 2014 From: suetp918 <@t> comcast.net (Sue) Date: Sun Aug 3 14:48:59 2014 Subject: [Histonet] IHC validation: In-Reply-To: <5E734B43-C215-46D7-9D1A-C03644D18BDB@gmail.com> Message-ID: <290973911.895484.1407095311425.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> All you would need to do is id the tissue and dx on your paperwork. it should not be any different than running each blocks alone. susan T. paturzo TJU From Richard.Cartun <@t> hhchealth.org Sun Aug 3 19:08:08 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Sun Aug 3 19:08:15 2014 Subject: [Histonet] IHC validation: In-Reply-To: <5E734B43-C215-46D7-9D1A-C03644D18BDB@gmail.com> References: <5E734B43-C215-46D7-9D1A-C03644D18BDB@gmail.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355C2A4E@HHCEXCHMB03.hhcsystem.org> Many labs have turned to tissue microarrays (TMAs) for validating antibodies for IHC testing as a matter of convenience and lower cost. Ideally, the tissues used to make the TMA(s) should be fixed in the formalin that is used in your laboratory, as well as processed in your Histology laboratory under identical conditions as those used for patient specimens. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Sunday, August 03, 2014 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC validation: Does anyone have experience validating antibodies using tissue microarrays? Is this a preferred method? I was thinking of punching different tissues/cases, then embedding them in one block, and then validating the antibody. Please advise. Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From lpjones <@t> srhs-pa.org Mon Aug 4 08:51:17 2014 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Mon Aug 4 08:51:20 2014 Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!! Message-ID: >From Gayle's very knowledgable post about storage of Schiffs: "However, if I had to have immediate PAS staining in a busty lab, " We would like a definition of "busty", please!! Thank you for the laugh! ________________________________ Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From gayle.callis <@t> bresnan.net Mon Aug 4 09:36:12 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Aug 4 09:36:22 2014 Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!! In-Reply-To: References: Message-ID: <000001cfaff1$7241fca0$56c5f5e0$@bresnan.net> You have me laughing at myself!!! I'll blame the computer for the typo error and spend the rest of my day laughing at myself. This isn't the first time this has happened in one of my posts. Whatever you do, do NOT make the mistake of misspelling "fuchsin" or your good friends will come back and haunt you with that f-bomb mistake. I laughed even harder then but am more careful when typing out that word. Busy? I don't think I was referring to any feminine anatomical features we all know so well. I am quite sure some histo guys are laughing too. Still chuckling and glad I provided some unintentional amusement in that lengthy post. Gayle Callis ;) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, August 04, 2014 7:51 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!! >From Gayle's very knowledgable post about storage of Schiffs: "However, if I had to have immediate PAS staining in a busty lab, " We would like a definition of "busty", please!! Thank you for the laugh! ________________________________ Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Mon Aug 4 10:39:36 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Aug 4 10:39:44 2014 Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!! In-Reply-To: <000001cfaff1$7241fca0$56c5f5e0$@bresnan.net> References: <000001cfaff1$7241fca0$56c5f5e0$@bresnan.net> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF9F27E8C@MERCERMAIL.MercerU.local> Oh where is that Like button? sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Monday, August 04, 2014 10:36 AM To: 'Jones, Laura'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gayle Callis, You Made Our Monday Morning!! You have me laughing at myself!!! I'll blame the computer for the typo error and spend the rest of my day laughing at myself. This isn't the first time this has happened in one of my posts. Whatever you do, do NOT make the mistake of misspelling "fuchsin" or your good friends will come back and haunt you with that f-bomb mistake. I laughed even harder then but am more careful when typing out that word. Busy? I don't think I was referring to any feminine anatomical features we all know so well. I am quite sure some histo guys are laughing too. Still chuckling and glad I provided some unintentional amusement in that lengthy post. Gayle Callis ;) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, August 04, 2014 7:51 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!! >From Gayle's very knowledgable post about storage of Schiffs: "However, if I had to have immediate PAS staining in a busty lab, " We would like a definition of "busty", please!! Thank you for the laugh! ________________________________ Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Max.Mirot <@t> svrhc.org Mon Aug 4 12:05:59 2014 From: Max.Mirot <@t> svrhc.org (Dr. Max Mirot) Date: Mon Aug 4 12:05:37 2014 Subject: [Histonet] Histotech Position South of Tuscon Message-ID: We are looking for a full time Histotech for our pathology laboratory. We are a family owed independent pathology laboratory. We provide pathology services to several hospitals and a number of physician offices in our area. The lab is pathologist owned and managed. You would be joining another experienced Histotech. The lab recently moved to a new facility and features the latest Ventana equipment. We seek someone with at least several years experience performing core histology procedures. Experience grossing small specimens would be helpful but not required for employment. Salary and benefits are competitive. Paid relocation. The lab is located is Sierra Vista, AZ about 80 south of Tucson. This location has some of the best weather in the continental USA. We average 10 degrees cooler than Tucson due our 5000 foot elevation. Please send your resume to: Max S. Mirot, M.D. max.mirot@svrhc.org From tbraud <@t> holyredeemer.com Mon Aug 4 12:30:16 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Aug 4 12:30:19 2014 Subject: [Histonet] RE: IHC Validation with TMAs In-Reply-To: <20140804161214.5A7CB1E8081@trendmess-svr.holyredeemer.local> References: <20140804161214.5A7CB1E8081@trendmess-svr.holyredeemer.local> Message-ID: I had to revalidate all of my antibodies when we changed our IHC platform. I used "store bought" TMAs and passed my CAP inspection with flying colors. The cost savings is enormous. Ideally, they should be processed the same as your test tissue, but since our CAP proficiency slides are not, I did not worry about it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. IHC validation: (Jb) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From jstaruk <@t> masshistology.com Mon Aug 4 12:54:11 2014 From: jstaruk <@t> masshistology.com (Mass Histology) Date: Mon Aug 4 12:54:13 2014 Subject: [Histonet] Looking for gram control tissue Message-ID: <012e01cfb00d$19890fb0$4c9b2f10$@masshistology.com> Hi all, I'm in need of a good gram control. Does anyone know of a tissue bank (? NSH) that offers control tissues? I have plenty of other positive control tissue I can donate but being in research (with no microbiology lab), a good gram control is eluding me. We tried the "Slim Jim" trick but only saw gram positive throughout. If anyone has an extra control (ruptured appendix), I'll gladly trade with what I have here. Thanks Jim _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com From j.rowaihi <@t> alborglaboratories.com Mon Aug 4 12:57:41 2014 From: j.rowaihi <@t> alborglaboratories.com (j.rowaihi) Date: Mon Aug 4 12:57:54 2014 Subject: =?UTF-8?Q?=D8=B1=D8=AF:_[Histonet]_RE:_IHC_Validation_with_TMAs?= Message-ID: Can you give us more details about the procedure or the SOPs because I just start the validation and I have no experience or support.? Regards Jamal Rowaihi Anatomic Pathology Supervisor Al Borg Medical Laboratories *Sent from my cell phoner* -------- ??????? ??????? -------- ??: Terri Braud ???????: ????/??/?? ?:?? ? (GMT+03:00) ???: histonet@lists.utsouthwestern.edu ???????: [Histonet] RE: IHC Validation with TMAs I had to revalidate all of my antibodies when we changed our IHC platform.? I used "store bought"? TMAs and passed my CAP inspection with flying colors. The cost savings is enormous.? Ideally, they should be processed the same as your test tissue, but since our CAP proficiency slides are not, I did not worry about it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: ?? 1. IHC validation: (Jb) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DShank <@t> chpnet.org Mon Aug 4 13:02:16 2014 From: DShank <@t> chpnet.org (Deborah Shank) Date: Mon Aug 4 13:02:28 2014 Subject: [Histonet] RE: IHC Validation with TMAs In-Reply-To: References: <20140804161214.5A7CB1E8081@trendmess-svr.holyredeemer.local> Message-ID: We make our own TMA, but would rather purchase for ease. Where did you buy yours? Deborah Shank, Manager Immunopathology, Flow Cytometry, Immunohistochemistry, FISH Tel 212 420 4049 FAX 212 420 4087 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Monday, August 04, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC Validation with TMAs I had to revalidate all of my antibodies when we changed our IHC platform. I used "store bought" TMAs and passed my CAP inspection with flying colors. The cost savings is enormous. Ideally, they should be processed the same as your test tissue, but since our CAP proficiency slides are not, I did not worry about it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 1. IHC validation: (Jb) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attachments are confidential and intended solely for the use of the individual or entity to which they are addressed. If you are not the intended recipient, you are prohibited from printing, copying, forwarding, saving, or otherwise using or relying upon them in any manner. Please notify the sender immediately if you have received this message by mistake and delete it from your system. From rob <@t> foliobio.com Mon Aug 4 13:12:44 2014 From: rob <@t> foliobio.com (Rob Day) Date: Mon Aug 4 13:13:00 2014 Subject: [Histonet] RE: IHC Validation with TMAs In-Reply-To: References: <20140804161214.5A7CB1E8081@trendmess-svr.holyredeemer.local> Message-ID: <064EDCB2-B404-4230-BC48-1BD98D5FA51E@foliobio.com> What kind of TMA are you looking for? You can get them from us if you like. Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 http://foliobio.com On Aug 4, 2014, at 2:02 PM, Deborah Shank wrote: > We make our own TMA, but would rather purchase for ease. Where did you buy yours? > > Deborah Shank, Manager > Immunopathology, Flow Cytometry, > Immunohistochemistry, FISH > Tel 212 420 4049 > FAX 212 420 4087 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud > Sent: Monday, August 04, 2014 1:30 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: IHC Validation with TMAs > > I had to revalidate all of my antibodies when we changed our IHC > platform. I used "store bought" TMAs and passed my CAP inspection with > flying colors. The cost savings is enormous. Ideally, they should be > processed the same as your test tissue, but since our CAP proficiency > slides are not, I did not worry about it. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > Today's Topics: > > 1. IHC validation: (Jb) > > outhwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Mon Aug 4 18:06:13 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Aug 4 18:06:35 2014 Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!! In-Reply-To: <000001cfaff1$7241fca0$56c5f5e0$@bresnan.net> References: <000001cfaff1$7241fca0$56c5f5e0$@bresnan.net> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00F7A84@xmdb04.nch.kids> Where is my "like" button??? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Tuesday, 5 August 2014 12:36 AM To: 'Jones, Laura'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gayle Callis, You Made Our Monday Morning!! You have me laughing at myself!!! I'll blame the computer for the typo error and spend the rest of my day laughing at myself. This isn't the first time this has happened in one of my posts. Whatever you do, do NOT make the mistake of misspelling "fuchsin" or your good friends will come back and haunt you with that f-bomb mistake. I laughed even harder then but am more careful when typing out that word. Busy? I don't think I was referring to any feminine anatomical features we all know so well. I am quite sure some histo guys are laughing too. Still chuckling and glad I provided some unintentional amusement in that lengthy post. Gayle Callis ;) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, August 04, 2014 7:51 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!! >From Gayle's very knowledgable post about storage of Schiffs: "However, if I had to have immediate PAS staining in a busty lab, " We would like a definition of "busty", please!! Thank you for the laugh! ________________________________ Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From jqb7 <@t> cdc.gov Mon Aug 4 18:36:32 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon Aug 4 18:36:47 2014 Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!! In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157E00F7A84@xmdb04.nch.kids> References: <000001cfaff1$7241fca0$56c5f5e0$@bresnan.net> <6D6BD1DE8A5571489398B392A38A7157E00F7A84@xmdb04.nch.kids> Message-ID: <3B2CD438E1628A41BD687E98B963B7811FDDD54E@EMBX-CHAM2.cdc.gov> I was thinking exactly the same thing but was afraid I was in the gutter..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Monday, August 04, 2014 7:06 PM To: 'gayle callis'; 'Jones, Laura'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gayle Callis, You Made Our Monday Morning!! Where is my "like" button??? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Tuesday, 5 August 2014 12:36 AM To: 'Jones, Laura'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Gayle Callis, You Made Our Monday Morning!! You have me laughing at myself!!! I'll blame the computer for the typo error and spend the rest of my day laughing at myself. This isn't the first time this has happened in one of my posts. Whatever you do, do NOT make the mistake of misspelling "fuchsin" or your good friends will come back and haunt you with that f-bomb mistake. I laughed even harder then but am more careful when typing out that word. Busy? I don't think I was referring to any feminine anatomical features we all know so well. I am quite sure some histo guys are laughing too. Still chuckling and glad I provided some unintentional amusement in that lengthy post. Gayle Callis ;) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, August 04, 2014 7:51 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Gayle Callis, You Made Our Monday Morning!! >From Gayle's very knowledgable post about storage of Schiffs: "However, if I had to have immediate PAS staining in a busty lab, " We would like a definition of "busty", please!! Thank you for the laugh! ________________________________ Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kristopher.Kalleberg <@t> unilever.com Tue Aug 5 07:18:11 2014 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Tue Aug 5 07:19:07 2014 Subject: [Histonet] whole slide scanners Message-ID: Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris From akbitting <@t> geisinger.edu Tue Aug 5 07:52:12 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Aug 5 07:52:20 2014 Subject: [Histonet] Desmoglein-3 Message-ID: <77F52EFAB8B1694B885E277C48FCD0F679E8E5AE@GHSEXMBX1W8K1V.geisinger.edu> Ventana Benchmark Ultra users: Where are you buying your Desmoglein-3? I need an IVD product. Thanks in advance, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From j.rowaihi <@t> alborglaboratories.com Tue Aug 5 08:13:50 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Tue Aug 5 08:11:21 2014 Subject: [Histonet] whole slide scanners In-Reply-To: References: Message-ID: <018201cfb0af$1ab21ec0$50165c40$@rowaihi@alborglaboratories.com> Did you find out about Aperio ePathology, it?s the most famous technology for slide imaging You can visit the link below http://www.leicabiosystems.com/pathology-imaging/epathology/ Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 3:18 PM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Tue Aug 5 09:14:29 2014 From: JWatson <@t> gnf.org (James Watson) Date: Tue Aug 5 09:14:34 2014 Subject: [Histonet] RE: whole slide scanners In-Reply-To: References: Message-ID: The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmire <@t> cvpath.org Tue Aug 5 09:36:41 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Tue Aug 5 09:36:49 2014 Subject: [Histonet] Exakt tech Message-ID: <951113C1-30C3-450A-A94B-87A4DBE42B96@cvpath.org> Hello Netters, Anyone in Histoland know of a good Exakt tech? It is a very specialized skill and I need to identify some individuals that have this ability. Thanks for your input Ronda From liz <@t> premierlab.com Tue Aug 5 10:15:53 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Aug 5 10:15:57 2014 Subject: [Histonet] RE: whole slide scanners In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD44C@SBS2K8.premierlab.local> I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Tue Aug 5 10:33:06 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Aug 5 10:33:16 2014 Subject: [Histonet] control slides Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115766DB2@PEITHA.wad.pa-ucl.com> Good Morning- We keep a control tissue log that documents where the tissue comes from, what stains it is used and has been tested for, when each block is put into use and exhausted and pathologist of record. When a block goes into use, do you mark on each slide the date it was cut (for those control tissues that are cut ahead)? How long are slides good for? Thank you- Nancy Schmitt MLT, HT(ASCP) Histology Coordinator NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Clough <@t> medicine.tamhsc.edu Tue Aug 5 10:55:29 2014 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Tue Aug 5 10:55:37 2014 Subject: [Histonet] Protocol for decalcifying and processing large bone section. Message-ID: Hi everyone, I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube. Currently I?ve been decalcifying the samples in 0.5M EDTA which is taking along time to decalcify this being the 19th day. Any advise would be greatly appreciated! Sincerely, Bret Clough Texas A&M Health Science Center Temple, TX. From suetp918 <@t> comcast.net Tue Aug 5 11:11:36 2014 From: suetp918 <@t> comcast.net (Sue) Date: Tue Aug 5 11:11:52 2014 Subject: [Histonet] control slides In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115766DB2@PEITHA.wad.pa-ucl.com> Message-ID: <417989381.902663.1407255096475.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> We actually just stared keeping a log for special stains, always for IHC.? Our rule of thumb is that an IHC control slide cannot be oler than 6 months,? although we go through most of them much faster.? For the basic IHC, tonsil, fallopian tube etc we do not log the case information for IHC only specific antibody controls, HSV, HPV, etc. S. Paturzo TJHUH From rjbuesa <@t> yahoo.com Tue Aug 5 11:14:09 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 5 11:14:14 2014 Subject: [Histonet] Protocol for decalcifying and processing large bone section. In-Reply-To: References: Message-ID: <1407255249.4756.YahooMailNeo@web120404.mail.ne1.yahoo.com> As a general rule EDTA is used?for the gentle decalcification of small or delicate bones, such as bone marrow samples. For large and dense bones, like the one your are trying to decalcify, you should use a strong acid. In order to prevent having to prepare the decalcifying solution I always used a brand name "RDO". Ren? J.? On Tuesday, August 5, 2014 11:55 AM, "Clough, Bret" wrote: Hi everyone, I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube.? Currently I?ve been decalcifying the samples in 0.5M EDTA which is taking along time to decalcify this being the 19th day.? Any advise would be greatly appreciated! Sincerely, ? Bret Clough ? Texas A&M Health Science Center ? Temple, TX. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Tue Aug 5 11:15:24 2014 From: b427297 <@t> aol.com (William J. O'Connor III) Date: Tue Aug 5 11:15:28 2014 Subject: [Histonet] Protocol for decalcifying and processing large bone section. In-Reply-To: References: Message-ID: <8D17EDF98CC87B5-204C-2A84E@webmail-d261.sysops.aol.com> Is there a need to decalcify the femoral head intact? We typically cut large, unfixed veterinary bones with an Exakt band saw into 5 mm slices prior to fixation and decalcification. This ensures the bones are well fixed prior to decalcification. We decalcify the selected slices for about 24 hours in hcl or formic acid. JO'C -----Original Message----- From: Clough, Bret To: Histonet list serv. Sent: Tue, Aug 5, 2014 10:56 am Subject: [Histonet] Protocol for decalcifying and processing large bone section. Hi everyone, I was hoping someone on histonet would being willing to help me by either haring their protocol or advising me on decalcifying and processing large bone ections. The bone sections are from the femural head of sheep measuring roughly cm x 1 1/2 cm cube. Currently I?ve been decalcifying the samples in 0.5M EDTA hich is taking along time to decalcify this being the 19th day. Any advise ould be greatly appreciated! incerely, Bret Clough Texas A&M Health Science Center Temple, TX. ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Tue Aug 5 11:33:23 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Tue Aug 5 11:33:35 2014 Subject: [Histonet] RE: whole slide scanners In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECD44C@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECD44C@SBS2K8.premierlab.local> Message-ID: I totally agree with both of you. We upgraded our Aperio (Leica) ScanScope XT to the new AT, which holds 400 slides and is much faster than the XT. Both can also scan 2x3 slides manually. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, August 05, 2014 8:16 AM To: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Tue Aug 5 12:01:16 2014 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Tue Aug 5 12:01:25 2014 Subject: [Histonet] Protocol for decalcifying and processing large bone section. In-Reply-To: References: Message-ID: <731BE09CDB19AA43AA8682199D42D31B1DC79F59@ADCEXCHANGE01.KaleidaHealth.org> I use 10% formic acid for large human bones but I don't think your bone sections are that large. Peggy DiCarlo Ortho Bone Lab BGMC 100 High St. Buffalo, NY 14203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clough, Bret Sent: Tuesday, August 05, 2014 11:55 To: Histonet list serv. Subject: [Histonet] Protocol for decalcifying and processing large bone section. Hi everyone, I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying the samples in 0.5M EDTA which is taking along time to decalcify this being the 19th day. Any advise would be greatly appreciated! Sincerely, Bret Clough Texas A&M Health Science Center Temple, TX. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida HealthÂ’s Technology Assistance Center at (716) 859-7777. From gu.lang <@t> gmx.at Tue Aug 5 12:12:42 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 5 12:12:59 2014 Subject: AW: [Histonet] RE: whole slide scanners In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECD44C@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECD44C@SBS2K8.premierlab.local> Message-ID: <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Tue Aug 5 13:05:49 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Aug 5 13:05:56 2014 Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 Message-ID: <12ECD7346266D74691EC2BFC75285E452F4B1C6D@BFL323E10.pathmdlabs.local> Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From liz <@t> premierlab.com Tue Aug 5 13:21:00 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Aug 5 13:21:06 2014 Subject: [Histonet] RE: whole slide scanners In-Reply-To: <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> References: <14E2C6176416974295479C64A11CB9AE019C79ECD44C@SBS2K8.premierlab.local> <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD460@SBS2K8.premierlab.local> We are a research based histology lab so we use scanning for several reasons: 1. We scan slides for clients so they can review their images remotely and capture snapshot images, annotate, etc- study sizes may range from 10 to 100's of slides 2. We scan all of our slides for IHC protocol development - our clients that request us to develop IHC staining protocols we scan slides so they can also view the slides and track our progress, plus this creates a great way for us to review the results too, we can easily determine the correct dilution by viewing multiple dilutions at the same time. 3. We scan all of our stained slides from the control blocks and these are linked to our control block database so anyone can view the scanned slide to see if the controls are working properly. 4. We have studies that require image analysis so all of the slides are scanned and then analyzed via a custom algorithm that will generate the data required for a particular study design. 5. We scan all of our IHC control slides so we can tract staining consistency These are just a few of the applications that we use WSI for. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: Tuesday, August 05, 2014 11:13 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From blayjorge <@t> gmail.com Tue Aug 5 13:39:18 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Tue Aug 5 13:39:24 2014 Subject: [Histonet] Listservers in microbiology Message-ID: Hello: I have found Histonet quite useful and the members with whom I have communicated helpful. Can someone recommend listservers with similar qualities that emphasize microbiology? If "yes", please feel free to email at blayjorge@gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From gu.lang <@t> gmx.at Tue Aug 5 13:54:07 2014 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 5 13:54:15 2014 Subject: AW: [Histonet] RE: whole slide scanners In-Reply-To: <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> References: <14E2C6176416974295479C64A11CB9AE019C79ECD44C@SBS2K8.premierlab.local> <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> Message-ID: <000a01cfb0de$a31e6460$e95b2d20$@gmx.at> Does anyone out there use digital pathology for routine diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Dienstag, 05. August 2014 19:13 An: 'Elizabeth Chlipala' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Max.Mirot <@t> svrhc.org Tue Aug 5 13:57:54 2014 From: Max.Mirot <@t> svrhc.org (Dr. Max Mirot) Date: Tue Aug 5 13:57:39 2014 Subject: [Histonet] Procedures and Policy manual needed Message-ID: I am attempting to re-write our procedure and policy manual. Our scope of service is routine anatomic pathology/histology and Non Gyn cytology. Right now the lab is CLIA certified. Anyone care to share their policy and procedure manual? I would like to look at a couple of nicely done manuals to serve as template. It can be for CAP not just CLIA. Send off list to me max.mirot@svrhc.org Thanks Max S. Mirot, M.D. From bcooper <@t> chla.usc.edu Tue Aug 5 14:07:05 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Aug 5 14:07:10 2014 Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F4B1C6D@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F4B1C6D@BFL323E10.pathmdlabs.local> Message-ID: I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, August 05, 2014 11:06 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Timothy.Morken <@t> ucsfmedctr.org Tue Aug 5 14:07:50 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Aug 5 14:08:16 2014 Subject: [Histonet] RE: whole slide scanners In-Reply-To: <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> References: <14E2C6176416974295479C64A11CB9AE019C79ECD44C@SBS2K8.premierlab.local> <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> Message-ID: <761E2B5697F795489C8710BCC72141FF36790AED@ex07.net.ucsf.edu> We are setting up Philips high speed slide scanners to scan frozen sections for remote Dx. One critical aspect is to get coverslip medium that dries fast... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, August 05, 2014 10:13 AM To: 'Elizabeth Chlipala' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Tue Aug 5 14:15:46 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Tue Aug 5 14:15:57 2014 Subject: [Histonet] Listservers in microbiology In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355C2F96@HHCEXCHMB03.hhcsystem.org> Our Director of Microbiology gave me this information: "Yes there is an excellent Listserver in microbiology but it is by invitation and requires ASM membership. Clinmicronetgroup." Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jorge A. Santiago-Blay Sent: Tuesday, August 05, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Listservers in microbiology Hello: I have found Histonet quite useful and the members with whom I have communicated helpful. Can someone recommend listservers with similar qualities that emphasize microbiology? If "yes", please feel free to email at blayjorge@gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From JWatson <@t> gnf.org Tue Aug 5 14:30:32 2014 From: JWatson <@t> gnf.org (James Watson) Date: Tue Aug 5 14:30:37 2014 Subject: [Histonet] RE: whole slide scanners In-Reply-To: <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> References: <14E2C6176416974295479C64A11CB9AE019C79ECD44C@SBS2K8.premierlab.local> <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> Message-ID: We are pharma research, about 40-45% of our slides are fluorescent. With 1 scanner. 2013 17,096 slides scanned 2013 20,501 slides scanned James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, August 05, 2014 10:13 AM To: 'Elizabeth Chlipala' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Aug 5 14:33:20 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Tue Aug 5 14:33:24 2014 Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 In-Reply-To: References: <12ECD7346266D74691EC2BFC75285E452F4B1C6D@BFL323E10.pathmdlabs.local>, Message-ID: Do the same as Brian. It is not that involved really, and its not worth getting a deficiency over. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: bcooper@chla.usc.edu > To: lcolbert@pathmdlabs.com; histonet@lists.utsouthwestern.edu > Date: Tue, 5 Aug 2014 19:07:05 +0000 > CC: > Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 > > I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Tuesday, August 05, 2014 11:06 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 > > Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Wed Aug 6 03:25:48 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Wed Aug 6 03:23:14 2014 Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 In-Reply-To: References: <12ECD7346266D74691EC2BFC75285E452F4B1C6D@BFL323E10.pathmdlabs.local>, Message-ID: <01f901cfb150$0864d680$192e8380$@rowaihi@alborglaboratories.com> I am also the same as Joelle & Brian, It never been questioned by CAP inspectors but since it's in the common checklist we have to follow that?s why we have a policy with simple procedure and record, the procedure almost the same as Brian procedure but some time we use the digital PH meter. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver Sent: Tuesday, August 05, 2014 10:33 PM To: Cooper, Brian; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 Do the same as Brian. It is not that involved really, and its not worth getting a deficiency over. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: bcooper@chla.usc.edu > To: lcolbert@pathmdlabs.com; histonet@lists.utsouthwestern.edu > Date: Tue, 5 Aug 2014 19:07:05 +0000 > CC: > Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 > > I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Tuesday, August 05, 2014 11:06 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 > > Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barryrittman <@t> gmail.com Wed Aug 6 07:02:10 2014 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Wed Aug 6 07:02:14 2014 Subject: [Histonet] Protocol for decalcifying and processing large bone section. In-Reply-To: <731BE09CDB19AA43AA8682199D42D31B1DC79F59@ADCEXCHANGE01.KaleidaHealth.org> References: <731BE09CDB19AA43AA8682199D42D31B1DC79F59@ADCEXCHANGE01.KaleidaHealth.org> Message-ID: I would recommend using Kristensen's "sodium formate/formic acid" mixture. This is more gentle than formic acid alone and almost as rapid. Barry On Tue, Aug 5, 2014 at 12:01 PM, DiCarlo, Margaret < MDiCarlo@kaleidahealth.org> wrote: > I use 10% formic acid for large human bones but I don't think your bone > sections are that large. > > Peggy DiCarlo > Ortho Bone Lab > BGMC > 100 High St. > Buffalo, NY 14203 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clough, Bret > Sent: Tuesday, August 05, 2014 11:55 > To: Histonet list serv. > Subject: [Histonet] Protocol for decalcifying and processing large bone > section. > > Hi everyone, > I was hoping someone on histonet would being willing to help me by either > sharing their protocol or advising me on decalcifying and processing large > bone sections. The bone sections are from the femural head of sheep > measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying > the samples in 0.5M EDTA which is taking along time to decalcify this being > the 19th day. Any advise would be greatly appreciated! > > > Sincerely, > Bret Clough > Texas A&M Health Science Center > Temple, TX. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The Keeping You Informed section of Kaleida Health`s website features a > wealth of information, stories and pictures about our valued workforce and > the tremendous momentum our organization is experiencing. Check us out at: > www.kaleidahealth.org/kyi > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, > or previous e-mail messages attached to it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, or a person responsible for > delivering it to the intended recipient, you are hereby notified that any > further review, disclosure, copying, dissemination, distribution, or use of > any of the information contained in or attached to this e-mail transmission > is strictly prohibited. If you have received this message in error, please > notify the sender immediately by e-mail, discard any paper copies, and > delete all electronic files of the message. If you are unable to contact > the sender or you are not sure as to whether you are the intended > recipient, please call Kaleida Health??s Technology Assistance Center at (716) > 859-7777. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mjones <@t> metropath.com Wed Aug 6 08:12:58 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Wed Aug 6 08:13:04 2014 Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 Message-ID: Ditto with Brian Cooper. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/5/14, 1:07 PM, "Cooper, Brian" wrote: >I've never been asked by a CAP auditor either, but our internal >inspectors used to inquire about this in a previous lab all the time. We >use Bromocresol Purple as an indicator here, and we have a simple log >sheet wherein our crew just tests one piece of cleaned glassware each >day. They write the type of glassware they test, and place a checkmark >in a "yellow" or "purple" column based upon what they see. If "purple," >they rewash, retest and document accordingly. > >Brian D. Cooper, HT (ASCP)CM | Histology Supervisor >Department of Pathology and Laboratory Medicine >Children's Hospital Los Angeles >4650 Sunset Blvd MS#43- Los Angeles, CA 90027 >Ph: 323.361.3357 Pager: 213-209-0184 >bcooper@chla.usc.edu > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie >Colbert >Sent: Tuesday, August 05, 2014 11:06 AM >To: Histonet Post (histonet@lists.utsouthwestern.edu) >Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 > >Do others in Histology follow these CAP Guidelines for cleaning >glassware? I'm not sure if this question actually applies to Histology. > I have never had an inspector ask me about it, but I'm wondering if >anyone has been questioned. > >Laurie Colbert, HT (ASCP) >Histology Supervisor >PATH MD >8158 Beverly Blvd. >Los Angeles, CA 90048 >(323) 648-3214 direct >(424) 245-7284 main lab > >The information contained in this transmission may contain privileged and >confidential information, including patient information protected by >federal and state privacy laws. It is intended only for the use of the >person(s) named above. If you are not the intended recipient, you are >hereby notified that any review, dissemination, distribution, or >duplication of this communication is strictly prohibited. If you are not >the intended recipient, please contact the sender by reply email and >destroy all copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------------------------------------------- >CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential >or legally privileged information. Any unauthorized review, use, >disclosure >or distribution is prohibited. If you are not the intended recipient, >please >contact the sender by reply e-mail and destroy all copies of this >original message. > >--------------------------------------------------------------------- > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans <@t> histologistics.com Wed Aug 6 08:50:03 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Wed Aug 6 08:50:07 2014 Subject: [Histonet] RI company seeks plastics company for research work Message-ID: Hello All, I have a company who needs: "a source of basic services for sectioning of GMA blocks containing biomaterial devices and subsequent H&E staining. The devices are cut for embedding to about 8mm in length, and contain adhesives, thermoplastic membranes, various scaffolds, and cells. Our group would do the embedding and transfer samples to you, and would be looking for about 25-50 samples/month." I would like to give him references so that he can email and or call places he chooses. Please email me and I will forward all information. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From Sarah.Dysart <@t> stdavids.com Wed Aug 6 09:51:09 2014 From: Sarah.Dysart <@t> stdavids.com (Sarah.Dysart@stdavids.com) Date: Wed Aug 6 09:51:14 2014 Subject: [Histonet] p16 Message-ID: <34C22BB94729434598D767D3F4EB95E0CFA038E519@FWDCWPMSGCMS03.hca.corpad.net> Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. Thanks Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 From madeathridge <@t> pastnashville.com Wed Aug 6 10:06:42 2014 From: madeathridge <@t> pastnashville.com (Mary Ann Deathridge) Date: Wed Aug 6 10:07:37 2014 Subject: [Histonet] neutralize formalin Message-ID: We pour our waste xylene/alcohol into a drum and the formalin waste in a seperate drum for a waste service company to remove. Problem is space to house 55 gal drums and we have more xylene/alc waste than formalin. Does anyone neutralize formalin and dispose of it down the drain? If so can you recommend the method/product utilized. Xylene recyler is not an option. Maryann Deathridge, BS, HT (ASCP) Lab Manager 615-298-4100, Fax: 615-298-4141 From thomas.huynh <@t> mdanderson.org Wed Aug 6 10:20:38 2014 From: thomas.huynh <@t> mdanderson.org (Huynh,Thomas) Date: Wed Aug 6 10:20:50 2014 Subject: [Histonet] RE: Decalcifying and Processing large bone Message-ID: Hi Bret We are using 10% Formic Acid as well and checking the decalcifying end-point by using the faxitron x-ray system. I work with bone cancer for 99% of the time and Formic Acid is safer than any other one. Thomas -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 06, 2014 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Protocol for decalcifying and processing large bone section. (DiCarlo, Margaret) 2. AW: [Histonet] RE: whole slide scanners (Gudrun Lang) 3. Glassware Cleaning, CAP Checklist Item GEN.41770 (Laurie Colbert) 4. RE: RE: whole slide scanners (Elizabeth Chlipala) 5. Listservers in microbiology (Jorge A. Santiago-Blay) 6. AW: [Histonet] RE: whole slide scanners (Gudrun Lang) 7. Procedures and Policy manual needed (Dr. Max Mirot) 8. RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Cooper, Brian) 9. RE: RE: whole slide scanners (Morken, Timothy) 10. RE: Listservers in microbiology (Cartun, Richard) 11. RE: RE: whole slide scanners (James Watson) 12. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Joelle Weaver) 13. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Jamal) 14. Re: Protocol for decalcifying and processing large bone section. (Barry Rittman) 15. Re: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Michael Ann Jones) 16. RI company seeks plastics company for research work (Hans B Snyder) 17. p16 (Sarah.Dysart@stdavids.com) 18. neutralize formalin (Mary Ann Deathridge) ---------------------------------------------------------------------- Message: 1 Date: Tue, 5 Aug 2014 17:01:16 +0000 From: "DiCarlo, Margaret" Subject: RE: [Histonet] Protocol for decalcifying and processing large bone section. To: "'Clough, Bret'" , Histonet list serv. Message-ID: <731BE09CDB19AA43AA8682199D42D31B1DC79F59@ADCEXCHANGE01.KaleidaHealth.org> Content-Type: text/plain; charset="us-ascii" I use 10% formic acid for large human bones but I don't think your bone sections are that large. Peggy DiCarlo Ortho Bone Lab BGMC 100 High St. Buffalo, NY 14203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clough, Bret Sent: Tuesday, August 05, 2014 11:55 To: Histonet list serv. Subject: [Histonet] Protocol for decalcifying and processing large bone section. Hi everyone, I was hoping someone on histonet would being willing to help me by either sharing their protocol or advising me on decalcifying and processing large bone sections. The bone sections are from the femural head of sheep measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying the samples in 0.5M EDTA which is taking along time to decalcify this being the 19th day. Any advise would be greatly appreciated! Sincerely, Bret Clough Texas A&M Health Science Center Temple, TX. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health??s Technology Assistance Center at (716) 859-7777. ------------------------------ Message: 2 Date: Tue, 5 Aug 2014 19:12:42 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] RE: whole slide scanners To: "'Elizabeth Chlipala'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <000a01cfb0d0$7948bd10$6bda3730$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 5 Aug 2014 18:05:49 +0000 From: Laurie Colbert Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 To: "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: <12ECD7346266D74691EC2BFC75285E452F4B1C6D@BFL323E10.pathmdlabs.local> Content-Type: text/plain; charset="us-ascii" Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ------------------------------ Message: 4 Date: Tue, 5 Aug 2014 12:21:00 -0600 From: Elizabeth Chlipala Subject: RE: [Histonet] RE: whole slide scanners To: Gudrun Lang Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD460@SBS2K8.premierlab.local> Content-Type: text/plain; charset="iso-8859-1" We are a research based histology lab so we use scanning for several reasons: 1. We scan slides for clients so they can review their images remotely and capture snapshot images, annotate, etc- study sizes may range from 10 to 100's of slides 2. We scan all of our slides for IHC protocol development - our clients that request us to develop IHC staining protocols we scan slides so they can also view the slides and track our progress, plus this creates a great way for us to review the results too, we can easily determine the correct dilution by viewing multiple dilutions at the same time. 3. We scan all of our stained slides from the control blocks and these are linked to our control block database so anyone can view the scanned slide to see if the controls are working properly. 4. We have studies that require image analysis so all of the slides are scanned and then analyzed via a custom algorithm that will generate the data required for a particular study design. 5. We scan all of our IHC control slides so we can tract staining consistency These are just a few of the applications that we use WSI for. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: Tuesday, August 05, 2014 11:13 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 5 Aug 2014 14:39:18 -0400 From: "Jorge A. Santiago-Blay" Subject: [Histonet] Listservers in microbiology To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hello: I have found Histonet quite useful and the members with whom I have communicated helpful. Can someone recommend listservers with similar qualities that emphasize microbiology? If "yes", please feel free to email at blayjorge@gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html ------------------------------ Message: 6 Date: Tue, 5 Aug 2014 20:54:07 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] RE: whole slide scanners To: "'Gudrun Lang'" Cc: Histonet@lists.utsouthwestern.edu Message-ID: <000a01cfb0de$a31e6460$e95b2d20$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Does anyone out there use digital pathology for routine diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang Gesendet: Dienstag, 05. August 2014 19:13 An: 'Elizabeth Chlipala' Cc: histonet@lists.utsouthwestern.edu Betreff: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 5 Aug 2014 18:57:54 +0000 From: "Dr. Max Mirot" Subject: [Histonet] Procedures and Policy manual needed To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I am attempting to re-write our procedure and policy manual. Our scope of service is routine anatomic pathology/histology and Non Gyn cytology. Right now the lab is CLIA certified. Anyone care to share their policy and procedure manual? I would like to look at a couple of nicely done manuals to serve as template. It can be for CAP not just CLIA. Send off list to me max.mirot@svrhc.org Thanks Max S. Mirot, M.D. ------------------------------ Message: 8 Date: Tue, 5 Aug 2014 19:07:05 +0000 From: "Cooper, Brian" Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 To: "Laurie Colbert" , "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset=iso-8859-1 I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, August 05, 2014 11:06 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ------------------------------ Message: 9 Date: Tue, 5 Aug 2014 19:07:50 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] RE: whole slide scanners To: "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF36790AED@ex07.net.ucsf.edu> Content-Type: text/plain; charset=iso-8859-1 We are setting up Philips high speed slide scanners to scan frozen sections for remote Dx. One critical aspect is to get coverslip medium that dries fast... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, August 05, 2014 10:13 AM To: 'Elizabeth Chlipala' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 5 Aug 2014 19:15:46 +0000 From: "Cartun, Richard" Subject: RE: [Histonet] Listservers in microbiology To: "Jorge A. Santiago-Blay" , "histonet@lists.utsouthwestern.edu" Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355C2F96@HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="utf-8" Our Director of Microbiology gave me this information: "Yes there is an excellent Listserver in microbiology but it is by invitation and requires ASM membership. Clinmicronetgroup." Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jorge A. Santiago-Blay Sent: Tuesday, August 05, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Listservers in microbiology Hello: I have found Histonet quite useful and the members with whom I have communicated helpful. Can someone recommend listservers with similar qualities that emphasize microbiology? If "yes", please feel free to email at blayjorge@gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 11 Date: Tue, 5 Aug 2014 19:30:32 +0000 From: James Watson Subject: RE: [Histonet] RE: whole slide scanners To: 'Gudrun Lang' , 'Elizabeth Chlipala' Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are pharma research, about 40-45% of our slides are fluorescent. With 1 scanner. 2013 17,096 slides scanned 2013 20,501 slides scanned James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, August 05, 2014 10:13 AM To: 'Elizabeth Chlipala' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: whole slide scanners Hi, for which purpose do you use the high troughput scanner. Archive or diagnostics? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Elizabeth Chlipala Gesendet: Dienstag, 05. August 2014 17:16 An: James Watson; 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: whole slide scanners I agree with James the type of scanner you need will be dependent upon your use cases and workload. We have an Aperio ScanScope XT which is an 120 slide scanner and that works well for us. I'm going to make a shameless plug for a workshop that myself, Bill DeSalvo and Jesus Ellin will be giving at the annual meeting in Austin this year, it's called Digital Pathology for the Histotech - A Guide to Implementation it's an all day workshop that is on Saturday the 23rd and it will cover all aspects of how to select a scanner, how to implement scanning, etc. It will be very valuable to histotechs that are already scanning and also techs that are new to the scanning process. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Tuesday, August 05, 2014 8:14 AM To: 'Kalleberg, Kristopher'; histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: whole slide scanners The scanner you get depends on your usage and volume. Three that I recommend are for high volume. Aperio, Good image quality, if you need to link to study metadata Spectrum software is great. Recommend for chromogen. Separate scanner for fluorescence, fluorescent scanner only holds 5 slides at a time. Hamamatsu Nanozoomer, What we use, we do high volume chromogen and fluorescence, excellent for both. Our NanoZoomer holds 210 slides for a chromogen run. Can load up about 100 fluorescent slides per night, auto focus works well. Philips, Chromogen only, very fast scanning with high quality scans. 60 slides per hour. There are many others on the market for smaller workloads. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kalleberg, Kristopher Sent: Tuesday, August 05, 2014 5:18 AM To: histonet-request@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] whole slide scanners Hello All, I am looking into the purchase of a whole slide scanner. If anyone could supply some recommendations it would be greatly appreciated. Thank you. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 5 Aug 2014 19:33:20 +0000 From: Joelle Weaver Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 To: "Cooper, Brian" , Laurie Colbert , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Do the same as Brian. It is not that involved really, and its not worth getting a deficiency over. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: bcooper@chla.usc.edu > To: lcolbert@pathmdlabs.com; histonet@lists.utsouthwestern.edu > Date: Tue, 5 Aug 2014 19:07:05 +0000 > CC: > Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 > > I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Tuesday, August 05, 2014 11:06 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 > > Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 6 Aug 2014 11:25:48 +0300 From: "Jamal" Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 To: "'Joelle Weaver'" , "'Cooper, Brian'" , "'Laurie Colbert'" , Message-ID: <01f901cfb150$0864d680$192e8380$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="windows-1256" I am also the same as Joelle & Brian, It never been questioned by CAP inspectors but since it's in the common checklist we have to follow that?s why we have a policy with simple procedure and record, the procedure almost the same as Brian procedure but some time we use the digital PH meter. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joelle Weaver Sent: Tuesday, August 05, 2014 10:33 PM To: Cooper, Brian; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 Do the same as Brian. It is not that involved really, and its not worth getting a deficiency over. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: bcooper@chla.usc.edu > To: lcolbert@pathmdlabs.com; histonet@lists.utsouthwestern.edu > Date: Tue, 5 Aug 2014 19:07:05 +0000 > CC: > Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 > > I've never been asked by a CAP auditor either, but our internal inspectors used to inquire about this in a previous lab all the time. We use Bromocresol Purple as an indicator here, and we have a simple log sheet wherein our crew just tests one piece of cleaned glassware each day. They write the type of glassware they test, and place a checkmark in a "yellow" or "purple" column based upon what they see. If "purple," they rewash, retest and document accordingly. > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Tuesday, August 05, 2014 11:06 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 > > Do others in Histology follow these CAP Guidelines for cleaning glassware? I'm not sure if this question actually applies to Histology. I have never had an inspector ask me about it, but I'm wondering if anyone has been questioned. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 6 Aug 2014 07:02:10 -0500 From: Barry Rittman Subject: Re: [Histonet] Protocol for decalcifying and processing large bone section. To: "Histonet list serv." Message-ID: Content-Type: text/plain; charset=UTF-8 I would recommend using Kristensen's "sodium formate/formic acid" mixture. This is more gentle than formic acid alone and almost as rapid. Barry On Tue, Aug 5, 2014 at 12:01 PM, DiCarlo, Margaret < MDiCarlo@kaleidahealth.org> wrote: > I use 10% formic acid for large human bones but I don't think your bone > sections are that large. > > Peggy DiCarlo > Ortho Bone Lab > BGMC > 100 High St. > Buffalo, NY 14203 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clough, Bret > Sent: Tuesday, August 05, 2014 11:55 > To: Histonet list serv. > Subject: [Histonet] Protocol for decalcifying and processing large bone > section. > > Hi everyone, > I was hoping someone on histonet would being willing to help me by either > sharing their protocol or advising me on decalcifying and processing large > bone sections. The bone sections are from the femural head of sheep > measuring roughly 1cm x 1 1/2 cm cube. Currently I've been decalcifying > the samples in 0.5M EDTA which is taking along time to decalcify this being > the 19th day. Any advise would be greatly appreciated! > > > Sincerely, > Bret Clough > Texas A&M Health Science Center > Temple, TX. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > The Keeping You Informed section of Kaleida Health`s website features a > wealth of information, stories and pictures about our valued workforce and > the tremendous momentum our organization is experiencing. Check us out at: > www.kaleidahealth.org/kyi > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, > or previous e-mail messages attached to it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, or a person responsible for > delivering it to the intended recipient, you are hereby notified that any > further review, disclosure, copying, dissemination, distribution, or use of > any of the information contained in or attached to this e-mail transmission > is strictly prohibited. If you have received this message in error, please > notify the sender immediately by e-mail, discard any paper copies, and > delete all electronic files of the message. If you are unable to contact > the sender or you are not sure as to whether you are the intended > recipient, please call Kaleida Health?????s Technology Assistance Center at (716) > 859-7777. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 15 Date: Wed, 6 Aug 2014 13:12:58 +0000 From: Michael Ann Jones Subject: Re: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770 To: "Cooper, Brian" , Laurie Colbert , "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain; charset="us-ascii" Ditto with Brian Cooper. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/5/14, 1:07 PM, "Cooper, Brian" wrote: >I've never been asked by a CAP auditor either, but our internal >inspectors used to inquire about this in a previous lab all the time. We >use Bromocresol Purple as an indicator here, and we have a simple log >sheet wherein our crew just tests one piece of cleaned glassware each >day. They write the type of glassware they test, and place a checkmark >in a "yellow" or "purple" column based upon what they see. If "purple," >they rewash, retest and document accordingly. > >Brian D. Cooper, HT (ASCP)CM | Histology Supervisor >Department of Pathology and Laboratory Medicine >Children's Hospital Los Angeles >4650 Sunset Blvd MS#43- Los Angeles, CA 90027 >Ph: 323.361.3357 Pager: 213-209-0184 >bcooper@chla.usc.edu > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie >Colbert >Sent: Tuesday, August 05, 2014 11:06 AM >To: Histonet Post (histonet@lists.utsouthwestern.edu) >Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770 > >Do others in Histology follow these CAP Guidelines for cleaning >glassware? I'm not sure if this question actually applies to Histology. > I have never had an inspector ask me about it, but I'm wondering if >anyone has been questioned. > >Laurie Colbert, HT (ASCP) >Histology Supervisor >PATH MD >8158 Beverly Blvd. >Los Angeles, CA 90048 >(323) 648-3214 direct >(424) 245-7284 main lab > >The information contained in this transmission may contain privileged and >confidential information, including patient information protected by >federal and state privacy laws. It is intended only for the use of the >person(s) named above. If you are not the intended recipient, you are >hereby notified that any review, dissemination, distribution, or >duplication of this communication is strictly prohibited. If you are not >the intended recipient, please contact the sender by reply email and >destroy all copies of the original message. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------------------------------------------- >CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential >or legally privileged information. Any unauthorized review, use, >disclosure >or distribution is prohibited. If you are not the intended recipient, >please >contact the sender by reply e-mail and destroy all copies of this >original message. > >--------------------------------------------------------------------- > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 6 Aug 2014 09:50:03 -0400 From: Hans B Snyder Subject: [Histonet] RI company seeks plastics company for research work To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Hello All, I have a company who needs: "a source of basic services for sectioning of GMA blocks containing biomaterial devices and subsequent H&E staining. The devices are cut for embedding to about 8mm in length, and contain adhesives, thermoplastic membranes, various scaffolds, and cells. Our group would do the embedding and transfer samples to you, and would be looking for about 25-50 samples/month." I would like to give him references so that he can email and or call places he chooses. Please email me and I will forward all information. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com ------------------------------ Message: 17 Date: Wed, 6 Aug 2014 09:51:09 -0500 From: Subject: [Histonet] p16 To: Message-ID: <34C22BB94729434598D767D3F4EB95E0CFA038E519@FWDCWPMSGCMS03.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. Thanks Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ------------------------------ Message: 18 Date: Wed, 6 Aug 2014 08:06:42 -0700 From: "Mary Ann Deathridge" Subject: [Histonet] neutralize formalin To: Message-ID: Content-Type: text/plain; charset="us-ascii" We pour our waste xylene/alcohol into a drum and the formalin waste in a seperate drum for a waste service company to remove. Problem is space to house 55 gal drums and we have more xylene/alc waste than formalin. Does anyone neutralize formalin and dispose of it down the drain? If so can you recommend the method/product utilized. Xylene recyler is not an option. Maryann Deathridge, BS, HT (ASCP) Lab Manager 615-298-4100, Fax: 615-298-4141 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 6 **************************************** From rjbuesa <@t> yahoo.com Wed Aug 6 11:07:48 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 6 11:07:51 2014 Subject: [Histonet] neutralize formalin In-Reply-To: References: Message-ID: <1407341268.29790.YahooMailNeo@web120401.mail.ne1.yahoo.com> Maryann: In spite of any seller's or user's opinion, I tried?ALL available formalin neutralizers available?in the market up to 2002 and was able to detect, after?the so called "neutralization", significant amounts of free formalin still present. Because of these?results I decided to keep sending my used formalin to a "disposal" company even when as established by law, MY LAB was responsible of any problems with that used formalin even when we did not dispose of it, meaning that the disposal companies are NOT liable for any problem. I recommend you to look into this "cradle-to-burial" responsibility for?your lab. in your state. We recycled our xylene until I developed a tissue processing method using only 2-propanol and mineral oil instead of ethanol and xylene and all our xylene related health problems disappeared with a significant improvement of?processed tissue quality. Ren? J.? On Wednesday, August 6, 2014 11:08 AM, Mary Ann Deathridge wrote: We? pour our waste xylene/alcohol into a drum and the formalin waste in a seperate drum for a waste service company to remove.? Problem is space to house 55 gal drums and we have more xylene/alc waste than formalin.? Does anyone neutralize formalin and dispose of it down the drain?? If so can you recommend? the method/product? utilized. Xylene recyler is not an option. ? ? Maryann Deathridge, BS, HT (ASCP)? Lab Manager? 615-298-4100, Fax: 615-298-4141 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Wed Aug 6 11:16:23 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Wed Aug 6 11:16:30 2014 Subject: [Histonet] Plus slides for Thermo SlideMate Message-ID: <9F3CFEE76E51B64991C7485270890B404990011E@EX5.lj.gnf.org> Dear all, For those of you using the SlideMate, can you give me a list of compatible slides for these units? Thanks! Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From hans <@t> histologistics.com Wed Aug 6 11:54:40 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Wed Aug 6 11:54:46 2014 Subject: [Histonet] Responses to Plastics Message-ID: Hello All, Thank you to all who responded to my query about the RI company. I have forwarded all your emails directly to the company. Who ever has the pleasure to service the company, you have my gratitude. Best Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From wbenton <@t> cua.md Wed Aug 6 12:00:24 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Aug 6 12:02:07 2014 Subject: [Histonet] RE: Plus slides for Thermo SlideMate In-Reply-To: <9F3CFEE76E51B64991C7485270890B404990011E@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B404990011E@EX5.lj.gnf.org> Message-ID: <0B8979A204680A42B93A52B486088CD93938636A77@CUAEXH1.GCU-MD.local> Teri, We have found that these slides work the best: Leica Part # 3800090 SLIDE WHITE Xtra Thermal (Color should not matter, we tried several different colors with good results) Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson [TJohnson@gnf.org] Sent: Wednesday, August 06, 2014 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Plus slides for Thermo SlideMate Dear all, For those of you using the SlideMate, can you give me a list of compatible slides for these units? Thanks! Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From algranth <@t> email.arizona.edu Wed Aug 6 12:54:05 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Wed Aug 6 12:54:10 2014 Subject: [Histonet] RE: Plus slides for Thermo SlideMate In-Reply-To: <0B8979A204680A42B93A52B486088CD93938636A77@CUAEXH1.GCU-MD.local> References: <9F3CFEE76E51B64991C7485270890B404990011E@EX5.lj.gnf.org> <0B8979A204680A42B93A52B486088CD93938636A77@CUAEXH1.GCU-MD.local> Message-ID: <8A2079E9-A3F1-468B-BBDB-234ED752A2B7@email.arizona.edu> I use the EPIC slides from CWS, Have always used them on this printer but have tried many other brands - these are the BEST. Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algranth@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 retiring in 30 days! From bburnett <@t> CapeCodHealth.org Wed Aug 6 14:13:55 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Wed Aug 6 14:14:04 2014 Subject: [Histonet] RE: p16 In-Reply-To: <34C22BB94729434598D767D3F4EB95E0CFA038E519@FWDCWPMSGCMS03.hca.corpad.net> References: <34C22BB94729434598D767D3F4EB95E0CFA038E519@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: I am looking for one as well, I wasn't sure if they owned the patent. Let me know if you find one. Thanks! Brandy Burnett, HTL (ASCP) Cape Cod Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sarah.Dysart@stdavids.com [Sarah.Dysart@stdavids.com] Sent: Wednesday, August 06, 2014 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. Thanks Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From Joyce.Weems <@t> emoryhealthcare.org Wed Aug 6 14:33:58 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Aug 6 14:34:06 2014 Subject: [Histonet] RE: p16 In-Reply-To: References: <34C22BB94729434598D767D3F4EB95E0CFA038E519@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: It is an exclusive Ventana product - sold through MTM Laboratories I believe it is. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Wednesday, August 06, 2014 3:14 PM To: Sarah.Dysart@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: p16 I am looking for one as well, I wasn't sure if they owned the patent. Let me know if you find one. Thanks! Brandy Burnett, HTL (ASCP) Cape Cod Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sarah.Dysart@stdavids.com [Sarah.Dysart@stdavids.com] Sent: Wednesday, August 06, 2014 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. Thanks Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rmire <@t> cvpath.org Wed Aug 6 14:36:42 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Wed Aug 6 14:36:54 2014 Subject: [Histonet] RE: p16 In-Reply-To: References: <34C22BB94729434598D767D3F4EB95E0CFA038E519@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: Before Ventana had p16, it was only available from MTM labs, if I recall correctly the name. I am not sure if Ventana is the exclusive vendor now or not. Good luck. On Aug 6, 2014, at 3:13 PM, "Burnett, Brandy" wrote: > I am looking for one as well, I wasn't sure if they owned the patent. Let me know if you find one. > Thanks! > > Brandy Burnett, HTL (ASCP) > Cape Cod Hospital > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Sarah.Dysart@stdavids.com [Sarah.Dysart@stdavids.com] > Sent: Wednesday, August 06, 2014 10:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] p16 > > Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. > Thanks > > Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) > Pathology Supervisor > St. David's North Austin Medical Center > 12221 North Mopac Expressway > Austin, Texas 78758 > (512)901-1220 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk@CapeCodHealth.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Aug 6 16:02:58 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Aug 6 16:03:39 2014 Subject: [Histonet] RE: p16 In-Reply-To: References: <34C22BB94729434598D767D3F4EB95E0CFA038E519@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: They bought the company! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire Sent: Wednesday, August 06, 2014 3:37 PM To: Burnett, Brandy Cc: histonet@lists.utsouthwestern.edu; Sarah.Dysart@stdavids.com Subject: Re: [Histonet] RE: p16 Before Ventana had p16, it was only available from MTM labs, if I recall correctly the name. I am not sure if Ventana is the exclusive vendor now or not. Good luck. On Aug 6, 2014, at 3:13 PM, "Burnett, Brandy" wrote: > I am looking for one as well, I wasn't sure if they owned the patent. Let me know if you find one. > Thanks! > > Brandy Burnett, HTL (ASCP) > Cape Cod Hospital > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of > Sarah.Dysart@stdavids.com [Sarah.Dysart@stdavids.com] > Sent: Wednesday, August 06, 2014 10:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] p16 > > Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. > Thanks > > Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. > David's North Austin Medical Center > 12221 North Mopac Expressway > Austin, Texas 78758 > (512)901-1220 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk@CapeCodHealth.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From chesarato <@t> hotmail.com Wed Aug 6 18:52:26 2014 From: chesarato <@t> hotmail.com (Cesar Francisco Romero) Date: Wed Aug 6 18:52:33 2014 Subject: [Histonet] RE: Decalcifying and Processing large bones Message-ID: For many years now I have used this protocol in Bone Marrow samples. The cortical of the bone is still a problem, but it can be solved with longer decal time. If you need to preserve morphology and antigenicity for Immunohistochemistry this is the best choice. Three Days Bouin?s Fixative ( From the very Beginning ), don?t use Formalin. Three Days of a Saturated Solution of EDTA ( Disodium Salt ) in distilled water. After this time put the blocks in the first alcohol and process as usual. Don?t put the blocks in formalin. EDTA alone or with Heating affects morphology very badly. For IHC Antigen Retrieval is the recommended or used for each antibody. Difficult ones as CD 5 or CD 23 work very well with this decal protocol. Cesar Romero Buenos Aires. Argentina. From jmoreira <@t> sidra.org Wed Aug 6 23:40:10 2014 From: jmoreira <@t> sidra.org (Joana Moreira) Date: Wed Aug 6 23:40:17 2014 Subject: [Histonet] AP Module of Cerner Millennium + AB&T and AP Module of Cerner Millenium + Cerebro Message-ID: Dear all, I was wondering if there's any one out there using the AP Module of the Cerner Millennium LIS with their Advanced Barcode and Tracking (AB&T) solution? And also, if there's anyone out there using the AP Module of the Cerner Millennium LIS interfaced with Leica's Cerebro Specimen tracking system? Many Thanks, Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From melissa.ng <@t> spdscientific.com Thu Aug 7 03:53:29 2014 From: melissa.ng <@t> spdscientific.com (Melissa Ng) Date: Thu Aug 7 03:53:39 2014 Subject: [Histonet] Bubbles in mounting media Message-ID: <39523B0CE90CC6458F92CAC795807902C238ECAC4D@vm023-exchange> Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa From melissa.ng <@t> spdscientific.com Thu Aug 7 03:59:34 2014 From: melissa.ng <@t> spdscientific.com (Melissa Ng) Date: Thu Aug 7 03:59:45 2014 Subject: [Histonet] Bubble problems! Message-ID: <39523B0CE90CC6458F92CAC795807902C238ECAC55@vm023-exchange> Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist From j.rowaihi <@t> alborglaboratories.com Thu Aug 7 05:04:14 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Thu Aug 7 05:01:46 2014 Subject: [Histonet] Bubbles in mounting media In-Reply-To: <39523B0CE90CC6458F92CAC795807902C238ECAC4D@vm023-exchange> References: <39523B0CE90CC6458F92CAC795807902C238ECAC4D@vm023-exchange> Message-ID: <029101cfb226$f2f5a540$d8e0efc0$@rowaihi@alborglaboratories.com> Hi Melissa Try to fill the tube of mounting media some hours before use. I use to fill it in the end of the day and in the next day the micro bubbles will be completely disappeared. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Ng Sent: Thursday, August 07, 2014 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubbles in mounting media Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bburnett <@t> CapeCodHealth.org Thu Aug 7 08:06:37 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Thu Aug 7 08:07:00 2014 Subject: [Histonet] RE: p16 In-Reply-To: References: <34C22BB94729434598D767D3F4EB95E0CFA038E519@FWDCWPMSGCMS03.hca.corpad.net> , Message-ID: Do you know what the dilution is from Biocare, and how many tests per vial? ________________________________________ From: Weems, Joyce K. [Joyce.Weems@emoryhealthcare.org] Sent: Wednesday, August 06, 2014 5:02 PM To: 'Ronda Mire'; Burnett, Brandy Cc: histonet@lists.utsouthwestern.edu; Sarah.Dysart@stdavids.com Subject: RE: [Histonet] RE: p16 They bought the company! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire Sent: Wednesday, August 06, 2014 3:37 PM To: Burnett, Brandy Cc: histonet@lists.utsouthwestern.edu; Sarah.Dysart@stdavids.com Subject: Re: [Histonet] RE: p16 Before Ventana had p16, it was only available from MTM labs, if I recall correctly the name. I am not sure if Ventana is the exclusive vendor now or not. Good luck. On Aug 6, 2014, at 3:13 PM, "Burnett, Brandy" wrote: > I am looking for one as well, I wasn't sure if they owned the patent. Let me know if you find one. > Thanks! > > Brandy Burnett, HTL (ASCP) > Cape Cod Hospital > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of > Sarah.Dysart@stdavids.com [Sarah.Dysart@stdavids.com] > Sent: Wednesday, August 06, 2014 10:51 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] p16 > > Does anyone know of a comparable clone to the Ventana p16 clone E6H4? I would prefer it to be undiluted (concentrate) so that we can titrate out the antibody ourselves. We need it to have publications that would back this up and/or stained slide pictures I can show our pathologist. > Thanks > > Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. > David's North Austin Medical Center > 12221 North Mopac Expressway > Austin, Texas 78758 > (512)901-1220 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk@CapeCodHealth.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Aug 7 08:11:31 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Aug 7 08:11:37 2014 Subject: [Histonet] RE: Bubble problems! In-Reply-To: <39523B0CE90CC6458F92CAC795807902C238ECAC55@vm023-exchange> References: <39523B0CE90CC6458F92CAC795807902C238ECAC55@vm023-exchange> Message-ID: <1407417081449.61772@accuratediagnosticlabs.com> Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Melissa Ng Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Aug 7 09:10:27 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Aug 7 09:10:40 2014 Subject: [Histonet] RE: Bubble problems! In-Reply-To: <1407417081449.61772@accuratediagnosticlabs.com> References: <39523B0CE90CC6458F92CAC795807902C238ECAC55@vm023-exchange> <1407417081449.61772@accuratediagnosticlabs.com> Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFDCF@EVS1.archildrens.org> Melissa we are having the same problem. We use a different mounting medium. I will try Barbara's advice and try adding a bit of xylene to the media. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, August 07, 2014 8:12 AM To: Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bubble problems! Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Melissa Ng Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From sbaldwin <@t> mhhcc.org Thu Aug 7 09:30:36 2014 From: sbaldwin <@t> mhhcc.org (Baldwin, Kathy) Date: Thu Aug 7 09:30:44 2014 Subject: [Histonet] Pay Wage Message-ID: <3550b65b54ad45f89c7bf41c645aa085@exch02.mhhcc.org> What is a good pay wage for a 25yr experience in Histology. Any good information out there ? Indiana based. S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbaldwin@mhhcc.org :) "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" Vision Statement: We are committed to being the preferred health and wellness provider; transforming lives through faith based, compassionate care. From craigak12 <@t> gmail.com Thu Aug 7 10:32:21 2014 From: craigak12 <@t> gmail.com (Jb) Date: Thu Aug 7 10:32:26 2014 Subject: [Histonet] IHC Negative slides: Message-ID: Is it necessary to run a negative slide per IHC case per CLIA when using polymer based detection? Does anyone have references? Thank you, Sent from my iPhone From Dorothy.L.Webb <@t> HealthPartners.Com Thu Aug 7 11:54:00 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Aug 7 11:54:15 2014 Subject: [Histonet] questions Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From algranth <@t> email.arizona.edu Thu Aug 7 12:07:40 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Thu Aug 7 12:07:56 2014 Subject: [Histonet] RE: questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> Message-ID: When I worked in clinical labs the order was first the priority cases (biopsies, bone marrows, etc.) then numerically. I remember once two physicians brought small biopsies and were in a hurry for the results, both wanted to be first out and I can still hear myself explaining to them that there could only be ONE first! Andi ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Webb, Dorothy L [Dorothy.L.Webb@HealthPartners.Com] Sent: Thursday, August 07, 2014 9:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Aug 7 12:17:47 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Aug 7 12:18:05 2014 Subject: [Histonet] RE: questions In-Reply-To: References: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> Message-ID: <761E2B5697F795489C8710BCC72141FF3679118B@ex07.net.ucsf.edu> "... and I can still hear myself explaining to them that there could only be ONE first!" Yes, DAILY!!! Every time someone wants to change things around so they get theirs first, this is our only defense as to why everyone can't get theirs first. 8 years of college doesn't seem to help here... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, August 07, 2014 10:08 AM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: questions When I worked in clinical labs the order was first the priority cases (biopsies, bone marrows, etc.) then numerically. I remember once two physicians brought small biopsies and were in a hurry for the results, both wanted to be first out and I can still hear myself explaining to them that there could only be ONE first! Andi ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Webb, Dorothy L [Dorothy.L.Webb@HealthPartners.Com] Sent: Thursday, August 07, 2014 9:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Aug 7 12:28:11 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Aug 7 12:28:26 2014 Subject: [Histonet] RE: questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFDD8@EVS1.archildrens.org> Our cases are cut by priority. And we don't process breasts. (children's hospital) Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, August 07, 2014 11:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Nancy_Schmitt <@t> pa-ucl.com Thu Aug 7 12:38:40 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Aug 7 12:38:48 2014 Subject: [Histonet] p16 Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115767F59@PEITHA.wad.pa-ucl.com> We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the container when received. Comes from Dako. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator Dubuque, IA ************************************************************* Message: 12 Date: Thu, 7 Aug 2014 13:06:37 +0000 From: "Burnett, Brandy" > Subject: RE: [Histonet] RE: p16 To: "Weems, Joyce K." >, "'Ronda Mire'" > Cc: "histonet@lists.utsouthwestern.edu" >, "Sarah.Dysart@stdavids.com" > Message-ID: > Content-Type: text/plain; charset="us-ascii" Do you know what the dilution is from Biocare, and how many tests per vial? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Nancy_Schmitt <@t> pa-ucl.com Thu Aug 7 12:46:58 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Aug 7 12:47:05 2014 Subject: [Histonet] questions Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115767F6C@PEITHA.wad.pa-ucl.com> 1. Our early (small biopsies)processor finishes at 4am. Anything with special instructions (Cyto blocks, liver bxs, pros bxs, Bone Marrow cases) are done first. Then just by numerical as they come out of the rack. Late processor finishes at 0630 and the first done are Breast bxs, sent node cases or other with special instructions. 2. We find that Small bxs and core bxs do well with less than 24 hours. Not sure if that is what you are looking for:) We have a primary embedder each day. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator ***************************************************************** Message: 17 Date: Thu, 7 Aug 2014 11:54:00 -0500 From: "Webb, Dorothy L" > Subject: [Histonet] questions To: "'histonet@lists.utsouthwestern.edu'" > Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From tmcampbell <@t> fmh.org Thu Aug 7 12:52:52 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Thu Aug 7 12:52:56 2014 Subject: [Histonet] p16 In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115767F59@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115767F59@PEITHA.wad.pa-ucl.com> Message-ID: <3566D9E34287BE4B95372179009446A0209F7FA3@EXCHANGE.fmhnt.fmh.org> Are you sure Dako has p16? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, August 07, 2014 1:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 We use p16 on the BondMax at a 1:4 dilution. There are 11.5 ml in the container when received. Comes from Dako. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator Dubuque, IA ************************************************************* Message: 12 Date: Thu, 7 Aug 2014 13:06:37 +0000 From: "Burnett, Brandy" > Subject: RE: [Histonet] RE: p16 To: "Weems, Joyce K." >, "'Ronda Mire'" > Cc: "histonet@lists.utsouthwestern.edu" >, "Sarah.Dysart@stdavids.com" > Message-ID: > Content-Type: text/plain; charset="us-ascii" Do you know what the dilution is from Biocare, and how many tests per vial? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Thu Aug 7 12:57:16 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Aug 7 12:57:22 2014 Subject: [Histonet] P16 Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115767FB6@PEITHA.wad.pa-ucl.com> Actually comes from Roche..............sorry about that. Nancy Schmitt MLT, HT(ASCP) Histology Coordinator NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From craigak12 <@t> gmail.com Thu Aug 7 13:12:03 2014 From: craigak12 <@t> gmail.com (Jb) Date: Thu Aug 7 13:12:10 2014 Subject: [Histonet] IHC QC forms: Message-ID: <67F2DF4F-D7C5-4E07-A2B1-41857796943D@gmail.com> Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone From mward <@t> wakehealth.edu Thu Aug 7 13:13:54 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Aug 7 13:14:02 2014 Subject: [Histonet] RE: questions In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679118B@ex07.net.ucsf.edu> References: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> <761E2B5697F795489C8710BCC72141FF3679118B@ex07.net.ucsf.edu> Message-ID: Sounds like we are all working at the same place! LOL I hear exactly the same questions.....is it something they teach to all pathology residents???? ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, August 07, 2014 1:18 PM To: 'Grantham, Andrea L - (algranth)'; Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: questions "... and I can still hear myself explaining to them that there could only be ONE first!" Yes, DAILY!!! Every time someone wants to change things around so they get theirs first, this is our only defense as to why everyone can't get theirs first. 8 years of college doesn't seem to help here... Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Thursday, August 07, 2014 10:08 AM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: questions When I worked in clinical labs the order was first the priority cases (biopsies, bone marrows, etc.) then numerically. I remember once two physicians brought small biopsies and were in a hurry for the results, both wanted to be first out and I can still hear myself explaining to them that there could only be ONE first! Andi ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Webb, Dorothy L [Dorothy.L.Webb@HealthPartners.Com] Sent: Thursday, August 07, 2014 9:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Aug 7 13:20:42 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Thu Aug 7 13:20:47 2014 Subject: [Histonet] questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> Message-ID: Priority driven block order, by specimen type/protocol, defined in SOP ( TAT for 'stat", ASAP & routine) Fixation is monitored for all tissues, just easier to do all the same, made entry field in LIS, and tracking log for manual back up for computer down times. Keep to CAP guidelines for breast, 6-72, disclaimers when needed for alternate fixation, decal, or missing times such as for referrals where it is not sent or recorded. I am the only, technical person, but this is what I do for SOP as of right now. Congrats on up coming retirement! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Dorothy.L.Webb@HealthPartners.Com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 7 Aug 2014 11:54:00 -0500 > Subject: [Histonet] questions > > I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. > 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? > > I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). > > Dorothy Webb, HT (ASCP) > Regions Histology Technical Specialist > 651-254-2962 > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Thu Aug 7 13:26:30 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Thu Aug 7 13:26:44 2014 Subject: [Histonet] IHC QC forms: In-Reply-To: <67F2DF4F-D7C5-4E07-A2B1-41857796943D@gmail.com> References: <67F2DF4F-D7C5-4E07-A2B1-41857796943D@gmail.com> Message-ID: <761E2B5697F795489C8710BCC72141FF3679120A@ex07.net.ucsf.edu> We only check and document control slide quality and need for repeat due to control failure. The pathologist reviewing the slides will send a QC form to us if there is a problem with case slides. Plus thye can enter a repeat for a poor stain as a ""repeat" and we can track the number of repeats in the system. It is actually one of our QA monitors and review of repeats is documented in our quarterly QA review meeting. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, August 07, 2014 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC forms: Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Thu Aug 7 13:38:32 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Thu Aug 7 13:35:10 2014 Subject: [Histonet] On the lighter side... Message-ID: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org From BSullivan <@t> virtua.org Thu Aug 7 13:51:28 2014 From: BSullivan <@t> virtua.org (Sullivan, Beatrice) Date: Thu Aug 7 13:51:35 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you From Matthew.Lauterbach <@t> avera.org Thu Aug 7 13:58:32 2014 From: Matthew.Lauterbach <@t> avera.org (Matthew Lauterbach) Date: Thu Aug 7 13:59:12 2014 Subject: [Histonet] Industry Standard Message-ID: <0FFD9B96579AA64D9A452478AFB414E20B0F126E@AHDC374ML4.averamail.net> Friends - One of our metrics we monitor in our lab is a receive to sign out time metric. We measure the time the specimen comes to the lab to the time the case is signed out by a pathologist. We normally run on average 31-32 hours per case for our inpatients (about 1200 cases per month) but I wanted to ask this group if you measure a similar metric. If you do, was is your turn around time? Any articles on the industry standard for this? Thank you so very much for any/all help you can provide. Best, Matthew J. Lauterbach, MBA, MLS (ASCP)CM Laboratory Services Manager Phlebotomy/Campus Services/Histology/POC Avera McKennan Region Phone: (605) 322-7126 Cell: (605) 496-1272 Fax: (605) 322-7083 From contact <@t> excaliburpathology.com Thu Aug 7 13:59:44 2014 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Aug 7 14:02:48 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> Message-ID: <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> 36 years. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech?? 36 years here.? You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Aug 7 14:05:19 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Aug 7 14:05:52 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F2B9F@evcspmbx2.ads.northwestern.edu> 29. I feel so young....... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sullivan, Beatrice Sent: Thursday, August 07, 2014 1:51 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Thu Aug 7 14:06:22 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Thu Aug 7 14:06:28 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org>, <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org>, <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> Message-ID: 39 years for me Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net > Date: Thu, 7 Aug 2014 11:59:44 -0700 > From: contact@excaliburpathology.com > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] On the lighter side... > > 36 years. > > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: "Sullivan, Beatrice" > To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" > Sent: Thursday, August 7, 2014 1:51 PM > Subject: RE: [Histonet] On the lighter side... > > > 44 years here. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. > > Thank you > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Thu Aug 7 14:09:28 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Thu Aug 7 14:09:40 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: 46 - but I was 2 when I took the sat for the exam Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From HornHV <@t> archildrens.org Thu Aug 7 14:09:40 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Aug 7 14:09:47 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFDDD@EVS1.archildrens.org> 41 years. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech?? 36 years here.? You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From PAMarcum <@t> uams.edu Thu Aug 7 14:09:40 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Aug 7 14:09:48 2014 Subject: [Histonet] RE: questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> References: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012404D645@Mail2Node2.ad.uams.edu> We embed and cut and by priority for: 1) bone marrows, 2) biopsies, 3) breast cases 4) surgicals, 5) placentas, 6) autopsies. RUSH cases for kidney, liver and heart take priority over all! Occasionally we will have requests for a specific case to be cut earlier than the usual priority list and will do that as needed. The cases are cut in numerical order for bone marrows and biopsies to make it easier to stain, organize and deliver them. (We don't have to look for them for an hour if they are in order and anything missing is immediately apparent.) Since we have four to five people cutting in the morning for surgicals we cut in numeric order by case and also alpha order again to help with staining, check out and delivery. Depending on the person sectioning faster or slower we may have cases out of numeric order at times on the stainer and at check out. We adhere strictly to the breast times for 6 to 72 hours in formalin as minimum and maximum limits. We would be prefer all breast have overnight fixation and that is not possible at times. We use the rule anything not in a cassette by 3PM cannot be processed overnight for breast. Then we know we have 6 hours based on time from gross to completion of formalin on the processors. The trick was to make them understand it had to be grossed and in a cassette by cut off not just soaking in formalin. We have residents. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, August 07, 2014 11:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions I have a couple of questions to ask where there is no right or wrong answer, just curious as to the process that other labs use. 1. After processing, how do you determine the order in which to cut and stain the blocks..numerical or priority driven? 2. Do you adhere to the 6-72 hours of fixation for breasts or make certain all breast tissue is fixed for a minimum time of, say, 24 hours but, of course no longer than 72? I appreciate your responses and thanks for your time!! I am retiring at the end of this year and trying to optimize some processes beforehand:). Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From suetp918 <@t> comcast.net Thu Aug 7 14:22:32 2014 From: suetp918 <@t> comcast.net (Sue) Date: Thu Aug 7 14:22:50 2014 Subject: [Histonet] questions In-Reply-To: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B7F7@HPEMX3.HealthPartners.int> Message-ID: <557590940.911237.1407439352911.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> So here at TJUH, we are color coded and all blocks other than routine and decals are cut in this manner.? One tech is assigned rapids (green), another tech is assigned bone marrow and cell blocks (yellow and blue) and one tech is assiged out satalite hospital (pink cassettes) All are completed by 10AM.? The fourth tech will start on biopsies (peach).? This is all done while embedding is still taking place.? Routine and decals are placed in order qc'd an distributed among all tech except the rapid tech.? They are all stained accordingly.? We do adhere the the proper fixation times for IHC.? Autopy cassettes are cut by a tech in the afternoon depending on who is available.? We do not have any lab aides so a tech is assigned to instrumentation, and one tech is assiged up front, that is staining, sorting and releasing of cases.? This tech is assisted by another tech when they have completed microtomy.? The tech that is in the front will cut biopsies, especially the ones for H-Pylori and than go to the front for the remainder of the shift. I know it sounds confusing but it actually works. Sue Paturzo TJUH From SLB <@t> stowers.org Thu Aug 7 14:23:16 2014 From: SLB <@t> stowers.org (Beckham, Sharon) Date: Thu Aug 7 14:24:25 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <25A4DE08332B19499904459F00AAACB719E29EFDDD@EVS1.archildrens.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> <25A4DE08332B19499904459F00AAACB719E29EFDDD@EVS1.archildrens.org> Message-ID: 41 years also!! Sharon Beckham Head, Histotechnology Stowers Institute for Medical Research Kansas City, MO -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, August 07, 2014 2:10 PM To: 'Paula Pierce'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 41 years. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech?? 36 years here.? You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Thu Aug 7 14:26:18 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Aug 7 14:26:27 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <25A4DE08332B19499904459F00AAACB719E29EFDDD@EVS1.archildrens.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> <25A4DE08332B19499904459F00AAACB719E29EFDDD@EVS1.archildrens.org> Message-ID: <571584342.155862.1407439578704.JavaMail.root@comcast.net> I just made 50.? Started early. ? Pam ----- Original Message ----- From: "Hazel V Horn" To: "Paula Pierce" , "Histonet" Sent: Thursday, August 7, 2014 2:09:40 PM Subject: RE: [Histonet] On the lighter side... 41 years. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ ?From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... ? 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech?? 36 years here.? You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Thu Aug 7 14:30:37 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Aug 7 14:30:44 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF9FE319F@MERCERMAIL.MercerU.local> 52 years, if we are all posting. Lol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech?? 36 years here.? You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cecystan76 <@t> gmail.com Thu Aug 7 14:36:45 2014 From: cecystan76 <@t> gmail.com (Cecilia A.) Date: Thu Aug 7 14:36:50 2014 Subject: [Histonet] formalin pigment and 10% NBF question Message-ID: Hi everyone, As I read and was told, formalin pigment is seen as a dark precipitate under light microscope. I also read that you can see this via polarizing microscope as it is birifringent. I'm wondering: does it first need to be seen under the light microscope, and birifringence is just a confirmation? Or, can you see its birifringence without needing to see the precipitates under the light microscope? With regards to 10% NBF, do you guys pH this fixative if it's commercially prepared? Is this always reliably neutral? Thank you for your time! Cecilia From michaela.tourville <@t> duke.edu Thu Aug 7 14:38:26 2014 From: michaela.tourville <@t> duke.edu (Michaela Lefaivre) Date: Thu Aug 7 14:38:35 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25BF9FE319F@MERCERMAIL.MercerU.local> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com>, <9BF995BC0E47744E9673A41486E24EE25BF9FE319F@MERCERMAIL.MercerU.local> Message-ID: 13 years Michaela LeFaivre BS, HTL (ASCP) CM Molecular Technician IV Molecular Pathology Rm 4344 Purple Zone 919-684-4303 919-684-5095 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Shirley A. Powell [POWELL_SA@mercer.edu] Sent: Thursday, August 07, 2014 3:30 PM To: Paula Pierce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 52 years, if we are all posting. Lol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From huimin.wang <@t> sickkids.ca Thu Aug 7 14:45:03 2014 From: huimin.wang <@t> sickkids.ca (Huimin Wang) Date: Thu Aug 7 14:46:07 2014 Subject: [Histonet] please remove me from the list Message-ID: <3406E4136F0871428A3491FE6642ED88790A9742@SKMBXX02.sickkids.ca> ________________________________ This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. From tbraud <@t> holyredeemer.com Thu Aug 7 14:46:07 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Aug 7 14:46:10 2014 Subject: [Histonet] RE: On the lighter side In-Reply-To: <20140807182015.425AD1E80B3@trendmess-svr.holyredeemer.local> References: <20140807182015.425AD1E80B3@trendmess-svr.holyredeemer.local> Message-ID: 34 years. Egads, I'm OLD! When did that happen? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Today's Topics: 12. On the lighter side... (Douglas Porter) Message: 12 Date: Thu, 7 Aug 2014 14:38:32 -0400 From: "Douglas Porter" Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You?? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ------------------------------ Message: 13 Date: Thu, 7 Aug 2014 18:51:28 +0000 From: "Sullivan, Beatrice" Subject: RE: [Histonet] On the lighter side... To: 'Douglas Porter' , "histonet@lists.utsouthwestern.edu" Message-ID: <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> Content-Type: text/plain; charset="us-ascii" 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From mburns <@t> atlanticurologyclinics.com Thu Aug 7 14:48:34 2014 From: mburns <@t> atlanticurologyclinics.com (Melissa Burns) Date: Thu Aug 7 14:48:48 2014 Subject: [Histonet] RE: On the lighter side In-Reply-To: References: <20140807182015.425AD1E80B3@trendmess-svr.holyredeemer.local>, Message-ID: <572D2908-A791-4DFD-8C67-09B14AFBC33C@atlanticurologyclinics.com> Well I'm just a baby.....in pathology 6 years...only! Sent from my iPhone > On Aug 7, 2014, at 3:47 PM, "Terri Braud" wrote: > > 34 years. Egads, I'm OLD! When did that happen? > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > Today's Topics: > > > 12. On the lighter side... (Douglas Porter) > > > Message: 12 > Date: Thu, 7 Aug 2014 14:38:32 -0400 > From: "Douglas Porter" > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You?? > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > > > ------------------------------ > > Message: 13 > Date: Thu, 7 Aug 2014 18:51:28 +0000 > From: "Sullivan, Beatrice" > Subject: RE: [Histonet] On the lighter side... > To: 'Douglas Porter' , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> > Content-Type: text/plain; charset="us-ascii" > > 44 years here. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas > Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > 517-372-5520 (phone) > 517-372-5540 (fax) > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it was sent. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jeff.Howery <@t> kadlecmed.org Thu Aug 7 14:53:48 2014 From: Jeff.Howery <@t> kadlecmed.org (Howery, Jeff L) Date: Thu Aug 7 14:53:55 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> Message-ID: 36 years here -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech?? 36 years here.? You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lhadley <@t> iupui.edu Thu Aug 7 14:56:17 2014 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Thu Aug 7 14:56:21 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: <8638FBDA16B0584D82AA21CD236FF97F5297E290@IU-MSSG-MBX110.ads.iu.edu> 30years tomorrow. Lee Ann Baldridge IUSM -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From web24 <@t> cornell.edu Thu Aug 7 15:01:41 2014 From: web24 <@t> cornell.edu (Willy Bemis) Date: Thu Aug 7 15:01:48 2014 Subject: [Histonet] Sources of LVN (low viscosity nitrocellulose) Message-ID: Historically, we used LVN (low viscosity nitrocellulose, also marketed as Celloidin and various other trade names) to embed specimens such as whole decalcified salmon heads for thick sectioning (30 to 100?m) using sliding microtomes such as the AO 860. I used to be able to purchase LVN powder wetted with alcohol from the Hercules Company. Because their LVN was not highly purified, it was much less expensive than Celloidin. It worked perfectly for our technique. Unfortunately, Hercules is no longer in business. Does anyone know of a commercial source for LVN? Willy From doug.porter <@t> caplab.org Thu Aug 7 15:10:46 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Thu Aug 7 15:07:20 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <8638FBDA16B0584D82AA21CD236FF97F5297E290@IU-MSSG-MBX110.ads.iu.edu> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <8638FBDA16B0584D82AA21CD236FF97F5297E290@IU-MSSG-MBX110.ads.iu.edu> Message-ID: <007401cfb27b$ad215c80$07641580$@caplab.org> Well...I appears most of us are right properly fixed. Good to see some "newbies" chiming in. This also shows that we need to get/keep more of the young ones interested in the field. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: Baldridge, Lee Ann [mailto:lhadley@iupui.edu] Sent: Thursday, August 07, 2014 3:56 PM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 30years tomorrow. Lee Ann Baldridge IUSM -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4744 / Virus Database: 3986/7994 - Release Date: 08/06/14 From relia1 <@t> earthlink.net Thu Aug 7 15:11:05 2014 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Aug 7 15:11:15 2014 Subject: [Histonet] On the lighter side... Message-ID: I have been recruiting exclusively in histology for 10 years. ? ; ) Thanks-Pam Right Place, Right Time, ?Right Opportunity.? Pam Barker RELIA Solutions.? relia1@earthlink.net? (407) 353-5070 cell/text Sent from my Galaxy S?III
-------- Original message --------
From: Douglas Porter
Date:08/07/2014 4:10 PM (GMT-05:00)
To: "'Baldridge, Lee Ann'" , histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
Well...I appears most of us are right properly fixed. Good to see some "newbies" chiming in. This also shows that we need to get/keep more of the young ones interested in the field. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: Baldridge, Lee Ann [mailto:lhadley@iupui.edu] Sent: Thursday, August 07, 2014 3:56 PM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 30years tomorrow. Lee Ann Baldridge IUSM -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4744 / Virus Database: 3986/7994 - Release Date: 08/06/14 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barryrittman <@t> gmail.com Thu Aug 7 15:16:54 2014 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Thu Aug 7 15:16:58 2014 Subject: [Histonet] Sources of LVN (low viscosity nitrocellulose) In-Reply-To: References: Message-ID: Technically LVN and celloidin are not quite the same. LVN (also known as gun cotton) is useful for large specimens -we used to use this for aborted fetal heads and large bone specimens. It penetrates much more rapidly that celloidin and is much easier and safer to handle. Tissues prepared especially in celloidin are magnificent due to the very low rate of shrinkage. The cost of safe transport of these is prohibitive. I am retired now but in the 1990s celloidin from Fisher was $1,200 a pound plus transport costs. Barry . On Thu, Aug 7, 2014 at 3:01 PM, Willy Bemis wrote: > Historically, we used LVN (low viscosity nitrocellulose, also marketed as > Celloidin and various other trade names) to embed specimens such as whole > decalcified salmon heads for thick sectioning (30 to 100?m) using sliding > microtomes such as the AO 860. > > I used to be able to purchase LVN powder wetted with alcohol from the > Hercules Company. Because their LVN was not highly purified, it was much > less expensive than Celloidin. It worked perfectly for our technique. > Unfortunately, Hercules is no longer in business. > > Does anyone know of a commercial source for LVN? > > Willy > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mturner <@t> CSILaboratories.com Thu Aug 7 15:19:27 2014 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Thu Aug 7 15:19:47 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: <643626B74DE2814D8537057F40E1A10B0CAD08DA@CSI-MX-NODEA.CSI-LABS.local> 35 years here. Histology has been very, very good to me! :-) Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, August 07, 2014 3:09 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 46 - but I was 2 when I took the sat for the exam Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbilaboratory <@t> gmail.com Thu Aug 7 15:23:08 2014 From: tbilaboratory <@t> gmail.com (Julie Bowman) Date: Thu Aug 7 15:23:17 2014 Subject: [Histonet] Water/Bubbles Message-ID: Hello all, Recently we have been experiencing a problem when running H&E slides on our Gemini stainer (Thermo Shandon Varistain). At the end of the run, small water droplets or possibly air bubbles are seen on the slides after taking the slides out of xylene. If they are coverslipped, a white haze will be seen around the specimen after a few minutes. Any suggestions of what causes this and how to correct it? Thank you! From akbitting <@t> geisinger.edu Thu Aug 7 15:23:14 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Thu Aug 7 15:23:20 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> Message-ID: <77F52EFAB8B1694B885E277C48FCD0F679E937A0@GHSEXMBX1W8K1V.geisinger.edu> 28 years here -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Howery, Jeff L Sent: Thursday, August 07, 2014 3:54 PM To: 'Paula Pierce'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 36 years here -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech?? 36 years here.? You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From brett_connolly <@t> merck.com Thu Aug 7 15:24:53 2014 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Aug 7 15:25:05 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: 34 years for me, but it seems like just yesterday it was 33.999 years. Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From lcolbert <@t> pathmdlabs.com Thu Aug 7 15:28:30 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Thu Aug 7 15:28:36 2014 Subject: [Histonet] Water/Bubbles In-Reply-To: References: Message-ID: <12ECD7346266D74691EC2BFC75285E452F4B253D@BFL323E10.pathmdlabs.local> Hi Julie, We have the Gemini stainer and have noticed that water gets trapped in the top of the slide rack. Make sure the level of your dehydrating alcohols after the eosin are high enough to cover the top of the rack, and then make sure your xylene level is high enough to get rid of all the alcohol. Also, avoid shaking the excess xylene off of the rack when you take it off the stainer before coverslipping. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julie Bowman Sent: Thursday, August 07, 2014 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water/Bubbles Hello all, Recently we have been experiencing a problem when running H&E slides on our Gemini stainer (Thermo Shandon Varistain). At the end of the run, small water droplets or possibly air bubbles are seen on the slides after taking the slides out of xylene. If they are coverslipped, a white haze will be seen around the specimen after a few minutes. Any suggestions of what causes this and how to correct it? Thank you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terra.Wineman <@t> novusint.com Thu Aug 7 15:44:17 2014 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Thu Aug 7 15:44:24 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: <1EB8F245A303564EADF12AC7022FA74DCA995CD1@novus-ex01> Celebrated 1yr HTL in July Terra Wineman, HTL (ASCP)CM Research Biologist, Nutritional Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Thursday, August 07, 2014 3:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 34 years for me, but it seems like just yesterday it was 33.999 years. Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly@merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathy.Machado <@t> LPNT.net Thu Aug 7 15:50:24 2014 From: Kathy.Machado <@t> LPNT.net (Kathy.Machado@LPNT.net) Date: Thu Aug 7 15:50:29 2014 Subject: [Histonet] re: on the light er side... Message-ID: 16 years!!! Kathy Machado, HTL Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3867 From vrivera <@t> westderm.com Thu Aug 7 15:57:32 2014 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Thu Aug 7 15:57:42 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: <3D4A471B82E7A44C87F6839732320D9F013FC79FA3@VSPDMS-ITEXMB02.DMS.COM> 15 Years and still going strong :) Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Aug 7 16:05:07 2014 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu Aug 7 16:05:14 2014 Subject: [Histonet] RE: re: on the light er side... In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B7811FDDEE4F@EMBX-CHAM2.cdc.gov> 36 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Machado@LPNT.net Sent: Thursday, August 07, 2014 4:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] re: on the light er side... 16 years!!! Kathy Machado, HTL Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3867 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smclaugh <@t> fhcrc.org Thu Aug 7 16:06:49 2014 From: smclaugh <@t> fhcrc.org (McLaughlin, Sharon R) Date: Thu Aug 7 16:06:58 2014 Subject: [Histonet] Re: Histonet Digest, Vol 129, Issue 10 In-Reply-To: <20140807202631.DF6DB6A224D@dogbert1.fhcrc.org> Message-ID: 44 years here On 8/7/14 1:26 PM, "histonet-request@lists.utsouthwestern.edu" wrote: >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. RE: questions (Marcum, Pamela A) > 2. Re: questions (Sue) > 3. RE: On the lighter side... (Beckham, Sharon) > 4. Re: On the lighter side... (Pam Marcum) > 5. RE: On the lighter side... (Shirley A. Powell) > 6. formalin pigment and 10% NBF question (Cecilia A.) > 7. RE: On the lighter side... (Michaela Lefaivre) > 8. please remove me from the list (Huimin Wang) > 9. RE: On the lighter side (Terri Braud) > 10. Re: RE: On the lighter side (Melissa Burns) > 11. RE: On the lighter side... (Howery, Jeff L) > 12. RE: On the lighter side... (Baldridge, Lee Ann) > 13. Sources of LVN (low viscosity nitrocellulose) (Willy Bemis) > 14. RE: On the lighter side... (Douglas Porter) > 15. RE: On the lighter side... (Pam Barker) > 16. Re: Sources of LVN (low viscosity nitrocellulose) (Barry Rittman) > 17. RE: On the lighter side... (Mark Turner) > 18. Water/Bubbles (Julie Bowman) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Thu, 7 Aug 2014 19:09:40 +0000 >From: "Marcum, Pamela A" >Subject: [Histonet] RE: questions >To: "Webb, Dorothy L" , > "'histonet@lists.utsouthwestern.edu'" > >Message-ID: > <41D3A1AF6FEF0643BDC89E0516A6EA32012404D645@Mail2Node2.ad.uams.edu> >Content-Type: text/plain; charset="us-ascii" > >We embed and cut and by priority for: 1) bone marrows, 2) biopsies, 3) >breast cases 4) surgicals, 5) placentas, 6) autopsies. RUSH cases for >kidney, liver and heart take priority over all! > >Occasionally we will have requests for a specific case to be cut earlier >than the usual priority list and will do that as needed. > >The cases are cut in numerical order for bone marrows and biopsies to >make it easier to stain, organize and deliver them. (We don't have to >look for them for an hour if they are in order and anything missing is >immediately apparent.) Since we have four to five people cutting in the >morning for surgicals we cut in numeric order by case and also alpha >order again to help with staining, check out and delivery. Depending on >the person sectioning faster or slower we may have cases out of numeric >order at times on the stainer and at check out. > >We adhere strictly to the breast times for 6 to 72 hours in formalin as >minimum and maximum limits. We would be prefer all breast have overnight >fixation and that is not possible at times. We use the rule anything not >in a cassette by 3PM cannot be processed overnight for breast. Then we >know we have 6 hours based on time from gross to completion of formalin >on the processors. The trick was to make them understand it had to be >grossed and in a cassette by cut off not just soaking in formalin. We >have residents. > >Pam Marcum > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, >Dorothy L >Sent: Thursday, August 07, 2014 11:54 AM >To: 'histonet@lists.utsouthwestern.edu' >Subject: [Histonet] questions > >I have a couple of questions to ask where there is no right or wrong >answer, just curious as to the process that other labs use. >1. After processing, how do you determine the order in which to cut and >stain the blocks..numerical or priority driven? 2. Do you adhere to the >6-72 hours of fixation for breasts or make certain all breast tissue is >fixed for a minimum time of, say, 24 hours but, of course no longer than >72? > >I appreciate your responses and thanks for your time!! I am retiring at >the end of this year and trying to optimize some processes beforehand:). > >Dorothy Webb, HT (ASCP) >Regions Histology Technical Specialist >651-254-2962 > > > > ________________________________ >This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they are >addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please >be advised that you have received this e-mail in error and that any use, >dissemination, forwarding, printing, or copying of this e-mail is >strictly prohibited. > >If you have received this communication in error, please return it to the >sender immediately and delete the original message and any copy of it >from your computer system. If you have any questions concerning this >message, please contact the sender. Disclaimer R001.0 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >---------------------------------------------------------------------- >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. > > > >------------------------------ > >Message: 2 >Date: Thu, 7 Aug 2014 19:22:32 +0000 (UTC) >From: Sue >Subject: Re: [Histonet] questions >To: Dorothy L Webb >Cc: histonet@lists.utsouthwestern.edu >Message-ID: > <557590940.911237.1407439352911.JavaMail.root@sz0028a.westchester.pa.mail >.comcast.net> > >Content-Type: text/plain; charset=utf-8 > > > > >So here at TJUH, we are color coded and all blocks other than routine and >decals are cut in this manner.?? One tech is assigned rapids (green), >another tech is assigned bone marrow and cell blocks (yellow and blue) >and one tech is assiged out satalite hospital (pink cassettes) All are >completed by 10AM.?? The fourth tech will start on biopsies (peach).?? >This is all done while embedding is still taking place.?? Routine and >decals are placed in order qc'd an distributed among all tech except the >rapid tech.?? They are all stained accordingly.?? We do adhere the the >proper fixation times for IHC.?? Autopy cassettes are cut by a tech in >the afternoon depending on who is available.?? We do not have any lab >aides so a tech is assigned to instrumentation, and one tech is assiged >up front, that is staining, sorting and releasing of cases.?? This tech >is assisted by another tech when they have completed microtomy.?? The >tech that is in the front will cut biopsies, especially the ones for H-P > ylori and than go to the front for the remainder of the shift. > > > >I know it sounds confusing but it actually works. > > > >Sue Paturzo >TJUH > > > >------------------------------ > >Message: 3 >Date: Thu, 7 Aug 2014 19:23:16 +0000 >From: "Beckham, Sharon" >Subject: RE: [Histonet] On the lighter side... >To: "'Horn, Hazel V'" , "'Paula Pierce'" > , "histonet@lists.utsouthwestern.edu" > >Message-ID: >Content-Type: text/plain; charset="iso-8859-1" > >41 years also!! > >Sharon Beckham >Head, Histotechnology >Stowers Institute for Medical Research >Kansas City, MO > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, >Hazel V >Sent: Thursday, August 07, 2014 2:10 PM >To: 'Paula Pierce'; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > >41 years. > >Hazel Horn >Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas >Children's Hospital >1 Children's Way | Slot 820| Little Rock, AR 72202 >501.364.4240 direct | 501.364.1241 fax >hornhv@archildrens.org >archildrens.org > > > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >Pierce >Sent: Thursday, August 07, 2014 2:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >36 years. > >? >Paula K. Pierce, HTL(ASCP)HT >President >Excalibur Pathology, Inc. >5830 N Blue Lake Dr. Please note new address! >Norman, OK 73069 >405-759-3953 Lab >405-759-7513 Fax >www.excaliburpathology.com > > >________________________________ > From: "Sullivan, Beatrice" >To: 'Douglas Porter' ; >"histonet@lists.utsouthwestern.edu" >Sent: Thursday, August 7, 2014 1:51 PM >Subject: RE: [Histonet] On the lighter side... > > >44 years here. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech?? 36 years here.? You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >This message, and any included attachments, are from Virtua Health or its >related affiliates and is intended only for the addressee(s). The >information contained herein is privileged, proprietary or may include >confidential information and/or protected patient health information. Any >unauthorized review, forwarding, printing, copying, distributing, or >otherwise disseminating or taking any action based on such information is >strictly prohibited. If you have received this message in error, or have >reason to believe you are not authorized to receive it, please delete >this message promptly and notify the sender by e-mail with a copy to >ISSECURITY@virtua.org. > >Thank you > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************** >The information contained in this message may be privileged and >confidential >and protected from disclosure. If the reader of this message is not the >intended recipient, or an employee or agent responsible for delivering >this >message to the intended recipient, you are hereby notified that any >dissemination, distribution or copying of this communication is strictly >prohibited. If you have received this communication in error, please >notify >us immediately by replying to the message and deleting it from your >computer. >Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 4 >Date: Thu, 7 Aug 2014 19:26:18 +0000 (UTC) >From: Pam Marcum >Subject: Re: [Histonet] On the lighter side... >To: Hazel V Horn >Cc: Histonet >Message-ID: <571584342.155862.1407439578704.JavaMail.root@comcast.net> >Content-Type: text/plain; charset=utf-8 > >I just made 50.?? Started early. >?? >Pam > >----- Original Message ----- > >From: "Hazel V Horn" >To: "Paula Pierce" , "Histonet" > >Sent: Thursday, August 7, 2014 2:09:40 PM >Subject: RE: [Histonet] On the lighter side... > >41 years. > >Hazel Horn >Supervisor of Histology/Autopsy/Transcription >Anatomic Pathology >Arkansas Children's Hospital >1 Children's Way | Slot 820| Little Rock, AR 72202 >501.364.4240 direct | 501.364.1241 fax >hornhv@archildrens.org >archildrens.org > > > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >Pierce >Sent: Thursday, August 07, 2014 2:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >36 years. > >?? >Paula K. Pierce, HTL(ASCP)HT >President >Excalibur Pathology, Inc. >5830 N Blue Lake Dr. Please note new address! >Norman, OK 73069 >405-759-3953 Lab >405-759-7513 Fax >www.excaliburpathology.com > > >________________________________ >??From: "Sullivan, Beatrice" >To: 'Douglas Porter' ; >"histonet@lists.utsouthwestern.edu" >Sent: Thursday, August 7, 2014 1:51 PM >Subject: RE: [Histonet] On the lighter side... >?? > >44 years here. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech??? 36 years here.?? You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? >?? >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org?? ?? ?? ?? ?? ?? ?? ?? ?? ?? ?? >?? ?? ?? ?? > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >This message, and any included attachments, are from Virtua Health or its >related affiliates and is intended only for the addressee(s). The >information contained herein is privileged, proprietary or may include >confidential information and/or protected patient health information. Any >unauthorized review, forwarding, printing, copying, distributing, or >otherwise disseminating or taking any action based on such information is >strictly prohibited. If you have received this message in error, or have >reason to believe you are not authorized to receive it, please delete >this message promptly and notify the sender by e-mail with a copy to >ISSECURITY@virtua.org. > >Thank you > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************************************************** >************************************** >The information contained in this message may be privileged and >confidential >and protected from disclosure. If the reader of this message is not the >intended recipient, or an employee or agent responsible for delivering >this >message to the intended recipient, you are hereby notified that any >dissemination, distribution or copying of this communication is strictly >prohibited. If you have received this communication in error, please >notify >us immediately by replying to the message and deleting it from your >computer. >Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 5 >Date: Thu, 7 Aug 2014 15:30:37 -0400 >From: "Shirley A. Powell" >Subject: RE: [Histonet] On the lighter side... >To: Paula Pierce , > "histonet@lists.utsouthwestern.edu" > >Message-ID: > <9BF995BC0E47744E9673A41486E24EE25BF9FE319F@MERCERMAIL.MercerU.local> >Content-Type: text/plain; charset="iso-8859-1" > >52 years, if we are all posting. Lol > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >Pierce >Sent: Thursday, August 07, 2014 3:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >36 years. > >? >Paula K. Pierce, HTL(ASCP)HT >President >Excalibur Pathology, Inc. >5830 N Blue Lake Dr. Please note new address! >Norman, OK 73069 >405-759-3953 Lab >405-759-7513 Fax >www.excaliburpathology.com > > >________________________________ > From: "Sullivan, Beatrice" >To: 'Douglas Porter' ; >"histonet@lists.utsouthwestern.edu" >Sent: Thursday, August 7, 2014 1:51 PM >Subject: RE: [Histonet] On the lighter side... > > >44 years here. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech?? 36 years here.? You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >This message, and any included attachments, are from Virtua Health or its >related affiliates and is intended only for the addressee(s). The >information contained herein is privileged, proprietary or may include >confidential information and/or protected patient health information. Any >unauthorized review, forwarding, printing, copying, distributing, or >otherwise disseminating or taking any action based on such information is >strictly prohibited. If you have received this message in error, or have >reason to believe you are not authorized to receive it, please delete >this message promptly and notify the sender by e-mail with a copy to >ISSECURITY@virtua.org. > >Thank you > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 6 >Date: Thu, 7 Aug 2014 14:36:45 -0500 >From: "Cecilia A." >Subject: [Histonet] formalin pigment and 10% NBF question >To: "Histonet (histonet@lists.utsouthwestern.edu)" > >Message-ID: > >Content-Type: text/plain; charset=UTF-8 > >Hi everyone, > >As I read and was told, formalin pigment is seen as a dark precipitate >under light microscope. I also read that you can see this via polarizing >microscope as it is birifringent. I'm wondering: does it first need to be >seen under the light microscope, and birifringence is just a confirmation? >Or, can you see its birifringence without needing to see the precipitates >under the light microscope? > >With regards to 10% NBF, do you guys pH this fixative if it's commercially >prepared? Is this always reliably neutral? > >Thank you for your time! >Cecilia > > >------------------------------ > >Message: 7 >Date: Thu, 7 Aug 2014 19:38:26 +0000 >From: Michaela Lefaivre >Subject: RE: [Histonet] On the lighter side... >To: "Shirley A. Powell" , Paula Pierce > , "histonet@lists.utsouthwestern.edu" > >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >13 years > >Michaela LeFaivre BS, HTL (ASCP) CM > >Molecular Technician IV > >Molecular Pathology > >Rm 4344 Purple Zone > >919-684-4303 > >919-684-5095 > >HIPAA Privacy Notification: This message and any accompanying documents >are covered by the Electronic Communications Privacy Act, 18 U.S.C. >2510-2521, and contain information intended for the specific individual >(s) only. This information is confidential. If you are not the intended >recipient or an agent responsible for delivering it to the intended >recipient, you are hereby notified that you have received this document >in error and that any review, dissemination, copying or the taking of any >action based on the contents of this information is strictly prohibited . >If you have received this communication in error, please notify us >immediately by e-mail, and delete the original message. > >________________________________________ >From: histonet-bounces@lists.utsouthwestern.edu >[histonet-bounces@lists.utsouthwestern.edu] on behalf of Shirley A. >Powell [POWELL_SA@mercer.edu] >Sent: Thursday, August 07, 2014 3:30 PM >To: Paula Pierce; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > >52 years, if we are all posting. Lol > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >Pierce >Sent: Thursday, August 07, 2014 3:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >36 years. > > >Paula K. Pierce, HTL(ASCP)HT >President >Excalibur Pathology, Inc. >5830 N Blue Lake Dr. Please note new address! >Norman, OK 73069 >405-759-3953 Lab >405-759-7513 Fax >www.excaliburpathology.com > > >________________________________ > From: "Sullivan, Beatrice" >To: 'Douglas Porter' ; >"histonet@lists.utsouthwestern.edu" >Sent: Thursday, August 7, 2014 1:51 PM >Subject: RE: [Histonet] On the lighter side... > > >44 years here. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech? 36 years here. You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >This message, and any included attachments, are from Virtua Health or its >related affiliates and is intended only for the addressee(s). The >information contained herein is privileged, proprietary or may include >confidential information and/or protected patient health information. Any >unauthorized review, forwarding, printing, copying, distributing, or >otherwise disseminating or taking any action based on such information is >strictly prohibited. If you have received this message in error, or have >reason to believe you are not authorized to receive it, please delete >this message promptly and notify the sender by e-mail with a copy to >ISSECURITY@virtua.org. > >Thank you > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------ > >Message: 8 >Date: Thu, 7 Aug 2014 19:45:03 +0000 >From: Huimin Wang >Subject: [Histonet] please remove me from the list >To: "Histonet@lists.utsouthwestern.edu" > >Message-ID: > <3406E4136F0871428A3491FE6642ED88790A9742@SKMBXX02.sickkids.ca> >Content-Type: text/plain; charset="us-ascii" > > > >________________________________ > >This e-mail may contain confidential, personal and/or health >information(information which may be subject to legal restrictions on >use, retention and/or disclosure) for the sole use of the intended >recipient. Any review or distribution by anyone other than the person for >whom it was originally intended is strictly prohibited. If you have >received this e-mail in error, please contact the sender and delete all >copies. > > >------------------------------ > >Message: 9 >Date: Thu, 7 Aug 2014 15:46:07 -0400 >From: "Terri Braud" >Subject: [Histonet] RE: On the lighter side >To: >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >34 years. Egads, I'm OLD! When did that happen? > >Terri L. Braud, HT(ASCP) >Anatomic Pathology Supervisor >Holy Redeemer Hospital Laboratory >1648 Huntingdon Pike >Meadowbrook, PA 19046 >Ph: 215-938-3676 >Fax: 215-938-3874 >Today's Topics: > > > 12. On the lighter side... (Douglas Porter) > > >Message: 12 >Date: Thu, 7 Aug 2014 14:38:32 -0400 >From: "Douglas Porter" >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech? 36 years here. You?? > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > > >------------------------------ > >Message: 13 >Date: Thu, 7 Aug 2014 18:51:28 +0000 >From: "Sullivan, Beatrice" >Subject: RE: [Histonet] On the lighter side... >To: 'Douglas Porter' , > "histonet@lists.utsouthwestern.edu" > >Message-ID: > ><6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> >Content-Type: text/plain; charset="us-ascii" > >44 years here. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech? 36 years here. You??? > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 >517-372-5520 (phone) >517-372-5540 (fax) >-------------------------------------------------------------------------- >------- > > > >CONFIDENTIALITY NOTICE: > >This E-Mail is intended only for the use of the individual or entity to >which >it was sent. It may contain information that is privileged and/or >confidential, >and the use or disclosure of such information may also be restricted >under applicable >federal and state law. If you received this communication in error, >please do not >distribute any part of it or retain any copies, and delete the original >E-Mail. >Please notify the sender of any error by E-Mail. > >Thank you for your cooperation. > > > > >------------------------------ > >Message: 10 >Date: Thu, 7 Aug 2014 19:48:34 +0000 >From: Melissa Burns >Subject: Re: [Histonet] RE: On the lighter side >To: Terri Braud >Cc: "histonet@lists.utsouthwestern.edu" > >Message-ID: > <572D2908-A791-4DFD-8C67-09B14AFBC33C@atlanticurologyclinics.com> >Content-Type: text/plain; charset="us-ascii" > >Well I'm just a baby.....in pathology 6 years...only! > >Sent from my iPhone > >> On Aug 7, 2014, at 3:47 PM, "Terri Braud" >>wrote: >> >> 34 years. Egads, I'm OLD! When did that happen? >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Holy Redeemer Hospital Laboratory >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> Ph: 215-938-3676 >> Fax: 215-938-3874 >> Today's Topics: >> >> >> 12. On the lighter side... (Douglas Porter) >> >> >> Message: 12 >> Date: Thu, 7 Aug 2014 14:38:32 -0400 >> From: "Douglas Porter" >> Subject: [Histonet] On the lighter side... >> >> How long have you been a registered histotech? 36 years here. You?? >> >> Douglas A. Porter, HT (ASCP) >> Grossing Technician >> IT Coordinator >> >> Cancer Registrar >> >> CAP-Lab, PLC >> 2508 South Cedar Street >> Lansing, MI 48910-3138 >> >> 517-372-5520 (phone) >> 517-372-5540 (fax) >> >> doug.porter@caplab.org >> >> www.caplab.org >> >> >> >> >> >> ------------------------------ >> >> Message: 13 >> Date: Thu, 7 Aug 2014 18:51:28 +0000 >> From: "Sullivan, Beatrice" >> Subject: RE: [Histonet] On the lighter side... >> To: 'Douglas Porter' , >> "histonet@lists.utsouthwestern.edu" >> >> Message-ID: >> >> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> >> Content-Type: text/plain; charset="us-ascii" >> >> 44 years here. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >> Porter >> Sent: Thursday, August 07, 2014 2:39 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] On the lighter side... >> >> How long have you been a registered histotech? 36 years here. You??? >> >> Douglas A. Porter, HT (ASCP) >> Grossing Technician >> IT Coordinator >> >> Cancer Registrar >> >> CAP-Lab, PLC >> 2508 South Cedar Street >> Lansing, MI 48910-3138 >> 517-372-5520 (phone) >> 517-372-5540 (fax) >> >>------------------------------------------------------------------------- >>-------- >> >> >> >> CONFIDENTIALITY NOTICE: >> >> This E-Mail is intended only for the use of the individual or entity to >>which >> it was sent. It may contain information that is privileged and/or >>confidential, >> and the use or disclosure of such information may also be restricted >>under applicable >> federal and state law. If you received this communication in error, >>please do not >> distribute any part of it or retain any copies, and delete the original >>E-Mail. >> Please notify the sender of any error by E-Mail. >> >> Thank you for your cooperation. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 11 >Date: Thu, 7 Aug 2014 12:53:48 -0700 >From: "Howery, Jeff L" >Subject: RE: [Histonet] On the lighter side... >To: 'Paula Pierce' , > "histonet@lists.utsouthwestern.edu" > >Message-ID: > >Content-Type: text/plain; charset="iso-8859-1" > >36 years here > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >Pierce >Sent: Thursday, August 07, 2014 12:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >36 years. > >? >Paula K. Pierce, HTL(ASCP)HT >President >Excalibur Pathology, Inc. >5830 N Blue Lake Dr. Please note new address! >Norman, OK 73069 >405-759-3953 Lab >405-759-7513 Fax >www.excaliburpathology.com > > >________________________________ > From: "Sullivan, Beatrice" >To: 'Douglas Porter' ; >"histonet@lists.utsouthwestern.edu" >Sent: Thursday, August 7, 2014 1:51 PM >Subject: RE: [Histonet] On the lighter side... > > >44 years here. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech?? 36 years here.? You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >This message, and any included attachments, are from Virtua Health or its >related affiliates and is intended only for the addressee(s). The >information contained herein is privileged, proprietary or may include >confidential information and/or protected patient health information. Any >unauthorized review, forwarding, printing, copying, distributing, or >otherwise disseminating or taking any action based on such information is >strictly prohibited. If you have received this message in error, or have >reason to believe you are not authorized to receive it, please delete >this message promptly and notify the sender by e-mail with a copy to >ISSECURITY@virtua.org. > >Thank you > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 12 >Date: Thu, 7 Aug 2014 19:56:17 +0000 >From: "Baldridge, Lee Ann" >Subject: RE: [Histonet] On the lighter side... >To: Douglas Porter , > "histonet@lists.utsouthwestern.edu" > >Message-ID: > <8638FBDA16B0584D82AA21CD236FF97F5297E290@IU-MSSG-MBX110.ads.iu.edu> >Content-Type: text/plain; charset="us-ascii" > >30years tomorrow. >Lee Ann Baldridge >IUSM > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech? 36 years here. You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 13 >Date: Thu, 7 Aug 2014 20:01:41 +0000 >From: Willy Bemis >Subject: [Histonet] Sources of LVN (low viscosity nitrocellulose) >To: "histonet@lists.utsouthwestern.edu" > >Message-ID: >Content-Type: text/plain; charset="iso-8859-1" > >Historically, we used LVN (low viscosity nitrocellulose, also marketed as >Celloidin and various other trade names) to embed specimens such as whole >decalcified salmon heads for thick sectioning (30 to 100?m) using sliding >microtomes such as the AO 860. > >I used to be able to purchase LVN powder wetted with alcohol from the >Hercules Company. Because their LVN was not highly purified, it was much >less expensive than Celloidin. It worked perfectly for our technique. >Unfortunately, Hercules is no longer in business. > >Does anyone know of a commercial source for LVN? > >Willy > > > > > >------------------------------ > >Message: 14 >Date: Thu, 7 Aug 2014 16:10:46 -0400 >From: "Douglas Porter" >Subject: RE: [Histonet] On the lighter side... >To: "'Baldridge, Lee Ann'" , > >Message-ID: <007401cfb27b$ad215c80$07641580$@caplab.org> >Content-Type: text/plain; charset="iso-8859-1" > >Well...I appears most of us are right properly fixed. Good to see some >"newbies" chiming in. This also shows that we need to get/keep more of >the >young ones interested in the field. > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator >Cancer Registrar > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > >doug.porter@caplab.org > >www.caplab.org >? >? >The information contained in this message may be privileged and/or >confidential and protected from disclosure. If the reader of this message >is >not the intended recipient, you are hereby notified that any >dissemination, >distribution, copying, forwarding or capture of this communication is >strictly prohibited. If you have received this communication in error, >please notify me immediately by return e-mail and delete this and all >copies. Thank-you. > > >-----Original Message----- >From: Baldridge, Lee Ann [mailto:lhadley@iupui.edu] >Sent: Thursday, August 07, 2014 3:56 PM >To: Douglas Porter; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > >30years tomorrow. >Lee Ann Baldridge >IUSM > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech? 36 years here. You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >----- >No virus found in this message. >Checked by AVG - www.avg.com >Version: 2014.0.4744 / Virus Database: 3986/7994 - Release Date: 08/06/14 > > > > >------------------------------ > >Message: 15 >Date: Thu, 07 Aug 2014 16:11:05 -0400 >From: Pam Barker >Subject: RE: [Histonet] On the lighter side... >To: Douglas Porter , "'Baldridge, Lee Ann'" > , histonet@lists.utsouthwestern.edu >Message-ID: >Content-Type: text/plain; charset=utf-8 > >I have been recruiting exclusively in histology for 10 years. ?? ; ) > > >Thanks-Pam > >Right Place, Right Time, ??Right Opportunity.?? > >Pam Barker RELIA Solutions.?? >relia1@earthlink.net?? >(407) 353-5070 cell/text >Sent from my Galaxy S??III > >
-------- Original message --------
From: Douglas Porter >
Date:08/07/2014 4:10 PM (GMT-05:00) >
To: "'Baldridge, Lee Ann'" , >histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On >the lighter side...
>
Well...I appears most of us are right properly fixed. Good to see >some >"newbies" chiming in. This also shows that we need to get/keep more of >the >young ones interested in the field. > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator >Cancer Registrar > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > >doug.porter@caplab.org > >www.caplab.org > > >The information contained in this message may be privileged and/or >confidential and protected from disclosure. If the reader of this message >is >not the intended recipient, you are hereby notified that any >dissemination, >distribution, copying, forwarding or capture of this communication is >strictly prohibited. If you have received this communication in error, >please notify me immediately by return e-mail and delete this and all >copies. Thank-you. > > >-----Original Message----- >From: Baldridge, Lee Ann [mailto:lhadley@iupui.edu] >Sent: Thursday, August 07, 2014 3:56 PM >To: Douglas Porter; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > >30years tomorrow. >Lee Ann Baldridge >IUSM > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech? 36 years here. You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >----- >No virus found in this message. >Checked by AVG - www.avg.com >Version: 2014.0.4744 / Virus Database: 3986/7994 - Release Date: 08/06/14 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >------------------------------ > >Message: 16 >Date: Thu, 7 Aug 2014 15:16:54 -0500 >From: Barry Rittman >Subject: Re: [Histonet] Sources of LVN (low viscosity nitrocellulose) >To: "histonet@lists.utsouthwestern.edu" > >Message-ID: > >Content-Type: text/plain; charset=UTF-8 > >Technically LVN and celloidin are not quite the same. >LVN (also known as gun cotton) is useful for large specimens -we used to >use this for aborted fetal heads and large bone specimens. It penetrates >much more rapidly that celloidin and is much easier and safer to handle. >Tissues prepared especially in celloidin are magnificent due to the very >low rate of shrinkage. >The cost of safe transport of these is prohibitive. >I am retired now but in the 1990s celloidin from Fisher was $1,200 a >pound >plus transport costs. >Barry > >. > > >On Thu, Aug 7, 2014 at 3:01 PM, Willy Bemis wrote: > >> Historically, we used LVN (low viscosity nitrocellulose, also marketed >>as >> Celloidin and various other trade names) to embed specimens such as >>whole >> decalcified salmon heads for thick sectioning (30 to 100??m) using >>sliding >> microtomes such as the AO 860. >> >> I used to be able to purchase LVN powder wetted with alcohol from the >> Hercules Company. Because their LVN was not highly purified, it was >>much >> less expensive than Celloidin. It worked perfectly for our technique. >> Unfortunately, Hercules is no longer in business. >> >> Does anyone know of a commercial source for LVN? >> >> Willy >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > >------------------------------ > >Message: 17 >Date: Thu, 7 Aug 2014 20:19:27 +0000 >From: Mark Turner >Subject: RE: [Histonet] On the lighter side... >To: "Weems, Joyce K." , 'Douglas > Porter' , "histonet@lists.utsouthwestern.edu" > >Message-ID: > <643626B74DE2814D8537057F40E1A10B0CAD08DA@CSI-MX-NODEA.CSI-LABS.local> >Content-Type: text/plain; charset="iso-8859-1" > >35 years here. Histology has been very, very good to me! :-) > >Mark Turner, Ph.D., HT(ASCP)QIHC >Manager, Histology/IHC >? > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, >Joyce K. >Sent: Thursday, August 07, 2014 3:09 PM >To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > >46 - but I was 2 when I took the sat for the exam > >Joyce Weems >Pathology Manager >678-843-7376 Phone >678-843-7831 Fax >joyce.weems@emoryhealthcare.org > > > >www.saintjosephsatlanta.org >5665 Peachtree Dunwoody Road >Atlanta, GA 30342 > >This e-mail, including any attachments is the property of Saint Joseph's >Hospital and is intended for the sole use of the intended recipient(s). >It may contain information that is privileged and confidential. Any >unauthorized review, use, disclosure, or distribution is prohibited. If >you are not the intended recipient, please delete this message, and reply >to the sender regarding the error in a separate email. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 2:39 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech? 36 years here. You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >________________________________ > >This e-mail message (including any attachments) is for the sole use of >the intended recipient(s) and may contain confidential and privileged >information. If the reader of this message is not the intended recipient, >you are hereby notified that any dissemination, distribution or copying >of this message (including any attachments) is strictly prohibited. > >If you have received this message in error, please contact the sender by >reply e-mail message and destroy all copies of the original message >(including attachments). > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 18 >Date: Thu, 7 Aug 2014 16:23:08 -0400 >From: Julie Bowman >Subject: [Histonet] Water/Bubbles >To: histonet@lists.utsouthwestern.edu >Message-ID: > >Content-Type: text/plain; charset=UTF-8 > >Hello all, > >Recently we have been experiencing a problem when running H&E slides on >our >Gemini stainer (Thermo Shandon Varistain). At the end of the run, small >water droplets or possibly air bubbles are seen on the slides after taking >the slides out of xylene. If they are coverslipped, a white haze will be >seen around the specimen after a few minutes. Any suggestions of what >causes this and how to correct it? Thank you! > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 129, Issue 10 >***************************************** From JMacDonald <@t> mtsac.edu Thu Aug 7 16:19:35 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Aug 7 16:29:27 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: 31 years Jennifer MacDonald From: "Douglas Porter" To: Date: 08/07/2014 11:36 AM Subject: [Histonet] On the lighter side... Sent by: histonet-bounces@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Thu Aug 7 16:34:10 2014 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Aug 7 16:34:14 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: Registered 4 years Doing histology 30 years. On Thu, Aug 7, 2014 at 5:19 PM, Jennifer MacDonald wrote: > 31 years > > Jennifer MacDonald > > > > From: "Douglas Porter" > To: > Date: 08/07/2014 11:36 AM > Subject: [Histonet] On the lighter side... > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From RCarter <@t> Cancer-test.com Thu Aug 7 16:36:24 2014 From: RCarter <@t> Cancer-test.com (Richard A. Carter) Date: Thu Aug 7 16:36:30 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: 28 years Rick Carter -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 7, 2014 2:20 PM To: Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 31 years Jennifer MacDonald From: "Douglas Porter" To: Date: 08/07/2014 11:36 AM Subject: [Histonet] On the lighter side... Sent by: histonet-bounces@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sherylmccandless <@t> harrisbb.com Thu Aug 7 17:21:25 2014 From: sherylmccandless <@t> harrisbb.com (Sheryl McCandless) Date: Thu Aug 7 17:21:33 2014 Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 11 In-Reply-To: <53e3f23b.ea13b60a.79be.49bcSMTPIN_ADDED_MISSING@mx.google.com> References: <53e3f23b.ea13b60a.79be.49bcSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <000901cfb28d$ed9520a0$c8bf61e0$@com> 10 years certified, 12 total! :) From CIngles <@t> uwhealth.org Thu Aug 7 17:39:39 2014 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Aug 7 17:39:43 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> , Message-ID: 15 years and over 10 certified HTL. Claire Ingles ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Richard A. Carter [RCarter@Cancer-test.com] Sent: Thursday, August 07, 2014 4:36 PM To: 'Jennifer MacDonald'; Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 28 years Rick Carter -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, August 7, 2014 2:20 PM To: Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 31 years Jennifer MacDonald From: "Douglas Porter" To: Date: 08/07/2014 11:36 AM Subject: [Histonet] On the lighter side... Sent by: histonet-bounces@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> gmail.com Thu Aug 7 17:44:05 2014 From: akemiat3377 <@t> gmail.com (Eileen Akemi Allison) Date: Thu Aug 7 17:44:10 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> , Message-ID: <5E4CAF81-76AD-40D5-AB99-9DF1D9BBA758@gmail.com> 49 years and 45 years certified as HT and 22 as HTL Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aallison@montereygi.com Tele: (831) 375-3577 X117 On Aug 7, 2014, at 3:39 PM, Ingles Claire wrote: > > 15 years and over 10 certified HTL. > Claire Ingles > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Richard A. Carter [RCarter@Cancer-test.com] > Sent: Thursday, August 07, 2014 4:36 PM > To: 'Jennifer MacDonald'; Douglas Porter > Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > 28 years > > Rick Carter > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald > Sent: Thursday, August 7, 2014 2:20 PM > To: Douglas Porter > Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] On the lighter side... > > 31 years > > Jennifer MacDonald > > > > From: "Douglas Porter" > To: > Date: 08/07/2014 11:36 AM > Subject: [Histonet] On the lighter side... > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.ng <@t> spdscientific.com Thu Aug 7 21:11:25 2014 From: melissa.ng <@t> spdscientific.com (Melissa Ng) Date: Thu Aug 7 21:11:34 2014 Subject: [Histonet] RE: Bubble problems! In-Reply-To: <25A4DE08332B19499904459F00AAACB719E29EFDCF@EVS1.archildrens.org> References: <39523B0CE90CC6458F92CAC795807902C238ECAC55@vm023-exchange> <1407417081449.61772@accuratediagnosticlabs.com> <25A4DE08332B19499904459F00AAACB719E29EFDCF@EVS1.archildrens.org> Message-ID: <39523B0CE90CC6458F92CAC795807902C238F718E2@vm023-exchange> Dear Hazel and Barbara, Thank you for your feedback. Wouldn't adding too much xylene result in drying effects post-coverslipping? Best thanks and regards, Melissa -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Thursday, 7 August, 2014 10:10 PM To: 'Barbara Tibbs'; Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: RE: Bubble problems! Melissa we are having the same problem. We use a different mounting medium. I will try Barbara's advice and try adding a bit of xylene to the media. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, August 07, 2014 8:12 AM To: Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bubble problems! Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Melissa Ng Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From nmhisto <@t> comcast.net Thu Aug 7 21:21:28 2014 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Thu Aug 7 21:21:55 2014 Subject: [Histonet] Eons in Histology In-Reply-To: <918553976.1130907.1407464473895.JavaMail.root@comcast.net> Message-ID: <831777913.1130973.1407464488206.JavaMail.root@comcast.net> I've been registered since 1969.? This thread is interesting and I'd like to see what those of us Who Have Been in this Game so Long have as registry numbers.? Mine is 04958 (I think...).? How many of you can beat that?? But who's counting?? I retired 2.5 years ago but I'll always be a histotech! From nmhisto <@t> comcast.net Thu Aug 7 21:32:48 2014 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Thu Aug 7 21:33:04 2014 Subject: [Histonet] Correction Message-ID: <2072541876.1133409.1407465168337.JavaMail.root@comcast.net> I must be getting old - my ASCP number is 04258, not 04958 (or whatever number I first wrote down)...? I hate it when that happens! From tpodawiltz <@t> lrgh.org Fri Aug 8 05:00:07 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Fri Aug 8 05:00:17 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: <38667E7FB77ECD4E91BFAEB8D9863863261589E632@LRGHEXVS1.practice.lrgh.org> 29 years. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From lpwenk <@t> sbcglobal.net Fri Aug 8 06:21:59 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Aug 8 06:22:06 2014 Subject: Fw: [Histonet] On the lighter side... Message-ID: I have been thinking of unsubscribing to Histonet, but then something like this comes along and I start to rethink my stance. I've never been a histotech, but spent 33 glorious years listening to Peggy and others talk about it. Some went over my head, but as time went by, less and less. As you can tell from her posts, she loved any opportunity to teach. And Histonet provided a large opportunity. I'd like to take this opportunity myself to thank everybody for all their good thoughts and wishes. And take a moment of silence in Austin (how would that go) for Peggy. Thanks and with lots of love, Lee Wenk PS: I'll probably unsubscribe (the correct way) sometime in the not too distant future. -----Original Message----- From: Podawiltz, Thomas Sent: Friday, August 08, 2014 6:00 AM To: Douglas Porter ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 29 years. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Aug 8 06:28:15 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Aug 8 06:28:20 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <38667E7FB77ECD4E91BFAEB8D9863863261589E632@LRGHEXVS1.practice.lrgh.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <38667E7FB77ECD4E91BFAEB8D9863863261589E632@LRGHEXVS1.practice.lrgh.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E391692514DA1@IBMB7Exchange.digestivespecialists.com> 41 in histology 32 registered. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Friday, August 08, 2014 6:00 AM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 29 years. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 <@t> va.gov Fri Aug 8 06:32:08 2014 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Fri Aug 8 06:32:46 2014 Subject: [Histonet] On the lighter side... Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760C478197@VHAV09MSGA3.v09.med.va.gov> 15 years as HT...26 years total in medicine started as an EMT and after some time discovered that patients in a jar is a blessing J thus the transfer to Pathology......and that is the rest of the story. Lisa White, HT(ASCP) Classification: Internal and External Use\\Not VA Sensitive This message has been categorized by White, Lisa M. on Friday, August 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 From b-frederick <@t> northwestern.edu Fri Aug 8 07:11:13 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Aug 8 07:11:19 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F2D41@evcspmbx2.ads.northwestern.edu> What's that in microns??? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Thursday, August 07, 2014 3:11 PM To: Douglas Porter; 'Baldridge, Lee Ann'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I have been recruiting exclusively in histology for 10 years. ? ; ) Thanks-Pam Right Place, Right Time, ?Right Opportunity.? Pam Barker RELIA Solutions. relia1@earthlink.net (407) 353-5070 cell/text Sent from my Galaxy S?III
-------- Original message --------
From: Douglas Porter
Date:08/07/2014 4:10 PM (GMT-05:00)
To: "'Baldridge, Lee Ann'" , histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
Well...I appears most of us are right properly fixed. Good to see some "newbies" chiming in. This also shows that we need to get/keep more of the young ones interested in the field. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: Baldridge, Lee Ann [mailto:lhadley@iupui.edu] Sent: Thursday, August 07, 2014 3:56 PM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 30years tomorrow. Lee Ann Baldridge IUSM -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4744 / Virus Database: 3986/7994 - Release Date: 08/06/14 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jburch01 <@t> gmail.com Fri Aug 8 07:12:06 2014 From: jburch01 <@t> gmail.com (Jim Burchette) Date: Fri Aug 8 07:12:12 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF39760C478197@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF39760C478197@VHAV09MSGA3.v09.med.va.gov> Message-ID: 40 blessed years that I would not trade for anything. I have met so many wonderful people (well, maybe a few not so wonderful...) and have learned so much. The good Lord has blessed me with a talent that I feel has allowed me to make a contribution to society. On Friday, August 8, 2014, White, Lisa M. wrote: > 15 years as HT...26 years total in medicine started as an EMT and after > some time discovered that patients in a jar is a blessing J thus the > transfer to Pathology......and that is the rest of the story. > > > > Lisa White, HT(ASCP) > > Classification: Internal and External Use\\Not VA Sensitive > This message has been categorized by White, Lisa M. on Friday, August > 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jim Burchette "Fly Fishing Bum" *<'(((><* From renafail2 <@t> gmail.com Fri Aug 8 07:40:42 2014 From: renafail2 <@t> gmail.com (Rena Fail) Date: Fri Aug 8 07:40:51 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <2B2ECF33934F5D4996D8BE03EFDF39760C478197@VHAV09MSGA3.v09.med.va.gov> Message-ID: 37 years On Friday, August 8, 2014, Jim Burchette wrote: > 40 blessed years that I would not trade for anything. I have met so many > wonderful people (well, maybe a few not so wonderful...) and have learned > so much. The good Lord has blessed me with a talent that I feel has allowed > me to make a contribution to society. > > On Friday, August 8, 2014, White, Lisa M. > wrote: > > > 15 years as HT...26 years total in medicine started as an EMT and after > > some time discovered that patients in a jar is a blessing J thus the > > transfer to Pathology......and that is the rest of the story. > > > > > > > > Lisa White, HT(ASCP) > > > > Classification: Internal and External Use\\Not VA Sensitive > > This message has been categorized by White, Lisa M. on Friday, August > > 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > Jim Burchette > "Fly Fishing Bum" *<'(((><* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Dolores_Fischer <@t> baxter.com Fri Aug 8 07:52:09 2014 From: Dolores_Fischer <@t> baxter.com (Fischer, Dolores) Date: Fri Aug 8 07:52:51 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <2B2ECF33934F5D4996D8BE03EFDF39760C478197@VHAV09MSGA3.v09.med.va.gov> Message-ID: <7CA324454CDDCA40A56FC3C51923C0E44B6E4AE6@BN1PRD9201MB008.026d.mgd.msft.net> 39 good years. My HT cert # is 7435. The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From joelleweaver <@t> hotmail.com Fri Aug 8 07:55:49 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Aug 8 07:55:57 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <2B2ECF33934F5D4996D8BE03EFDF39760C478197@VHAV09MSGA3.v09.med.va.gov>, , Message-ID: Jim, you and many others who have devoted so much of your lives to this "craft" & profession, are appreciated by all of us who have learned and benefited from your knowledge and years of experience over the years. Thanks to ALL of you who have stuck in there the decades of time needed to gather all those experiences, and especially for being willing to share with us. It is people like yourself, and Peggy, and many others too numerous to name in an email, that keep the rest of us inspired to keep going when we get weary! Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 8 Aug 2014 08:40:42 -0400 > From: renafail2@gmail.com > To: jburch01@gmail.com > Subject: Re: [Histonet] On the lighter side... > CC: histonet@lists.utsouthwestern.edu; Lisa.White3@va.gov > > 37 years > > On Friday, August 8, 2014, Jim Burchette wrote: > > > 40 blessed years that I would not trade for anything. I have met so many > > wonderful people (well, maybe a few not so wonderful...) and have learned > > so much. The good Lord has blessed me with a talent that I feel has allowed > > me to make a contribution to society. > > > > On Friday, August 8, 2014, White, Lisa M. > > wrote: > > > > > 15 years as HT...26 years total in medicine started as an EMT and after > > > some time discovered that patients in a jar is a blessing J thus the > > > transfer to Pathology......and that is the rest of the story. > > > > > > > > > > > > Lisa White, HT(ASCP) > > > > > > Classification: Internal and External Use\\Not VA Sensitive > > > This message has been categorized by White, Lisa M. on Friday, August > > > 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > -- > > Jim Burchette > > "Fly Fishing Bum" *<'(((><* > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Aug 8 07:55:44 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Aug 8 08:06:00 2014 Subject: [Histonet] RE: Correction In-Reply-To: <2072541876.1133409.1407465168337.JavaMail.root@comcast.net> References: <2072541876.1133409.1407465168337.JavaMail.root@comcast.net> Message-ID: 03824 - HT 01653 - HTL Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nmhisto@comcast.net Sent: Thursday, August 07, 2014 10:33 PM To: HISTONET Subject: [Histonet] Correction I must be getting old - my ASCP number is 04258, not 04958 (or whatever number I first wrote down)... I hate it when that happens! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From billodonnell <@t> catholichealth.net Fri Aug 8 08:20:49 2014 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Fri Aug 8 08:20:53 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <5E4CAF81-76AD-40D5-AB99-9DF1D9BBA758@gmail.com> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> , <5E4CAF81-76AD-40D5-AB99-9DF1D9BBA758@gmail.com> Message-ID: 30 years - Interesting demographics here, mainly (though not entirely) 'old timers' posting on this one. Logical conclusions: 1. It is a matter of pride or bragging rights that we geriatrics are posting or, 2. unnecessary shyness on the part of the younger members or, 3. the worst - very few younger followers of histonet Would love to see a whole bunch of newer techs posting - gives a great hope for the future of the profession -Bill This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From MAUGER <@t> email.chop.edu Fri Aug 8 09:00:32 2014 From: MAUGER <@t> email.chop.edu (Mauger, Joanne) Date: Fri Aug 8 09:00:36 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25BF9FE319F@MERCERMAIL.MercerU.local> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25BF9FE319F@MERCERMAIL.MercerU.local> Message-ID: <929548F049BC6D448DF9488B556449BE16562514@EXCMBXPW5.chop.edu> 35 long years!! Jo Mauger -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Thursday, August 07, 2014 3:31 PM To: Paula Pierce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 52 years, if we are all posting. Lol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech?? 36 years here.? You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmhisto <@t> comcast.net Fri Aug 8 09:28:20 2014 From: nmhisto <@t> comcast.net (nmhisto@comcast.net) Date: Fri Aug 8 09:28:37 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <5E4CAF81-76AD-40D5-AB99-9DF1D9BBA758@gmail.com> Message-ID: <411800840.1336526.1407508100512.JavaMail.root@comcast.net> I fear your last choice is the obvious conclusion.? We need a prime-time medical drama focusing on laboratorians!? We could create interest in histology if done correctly (no running a raw slide over a flame and placing it under the 'scope for diagnosis, for example). From cmiller <@t> gladstone.ucsf.edu Fri Aug 8 09:28:55 2014 From: cmiller <@t> gladstone.ucsf.edu (Caroline Miller) Date: Fri Aug 8 09:29:01 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <929548F049BC6D448DF9488B556449BE16562514@EXCMBXPW5.chop.edu> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <6932520047F7EE46B512E9801344F1608002AF89@ExchangeMB-1.Virtua.org> <1407437984.70083.YahooMailNeo@web162502.mail.bf1.yahoo.com> <9BF995BC0E47744E9673A41486E24EE25BF9FE319F@MERCERMAIL.MercerU.local> <929548F049BC6D448DF9488B556449BE16562514@EXCMBXPW5.chop.edu> Message-ID: <9334AAD6-753A-4381-B0C0-64EDF805AC1B@gladstone.ucsf.edu> 18 years! I was just talking to the Vector tech services yesterday how I had been using their products for that long and I am only 38! Started histo straight out of college. 5.5 years clinical BMS in London, 5.5 years research tech at UCSF and now 7 years in a research core, 4 as Director :) Thank you all for your great posts, I enjoy them immensely, especially Peggy's wisdom (thanks Lee for sticking around a little while, and thanks for Peggy)! Happy Friday to you all! Caroline Caroline Miller Director, Histology and Light Microscopy Core Gladstone Institutes www.gladstoneinstitutes.org Tel: 415 7342566 Cell: 415 2187297 > On Aug 8, 2014, at 7:00 AM, "Mauger, Joanne" wrote: > > 35 long years!! > Jo Mauger > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell > Sent: Thursday, August 07, 2014 3:31 PM > To: Paula Pierce; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > 52 years, if we are all posting. Lol > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce > Sent: Thursday, August 07, 2014 3:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] On the lighter side... > > 36 years. > > > Paula K. Pierce, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Dr. Please note new address! > Norman, OK 73069 > 405-759-3953 Lab > 405-759-7513 Fax > www.excaliburpathology.com > > > ________________________________ > From: "Sullivan, Beatrice" > To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" > Sent: Thursday, August 7, 2014 1:51 PM > Subject: RE: [Histonet] On the lighter side... > > > 44 years here. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. > > Thank you > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Fri Aug 8 09:32:46 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Aug 8 09:32:55 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> "36" years; however, not registered. Hopefully, I can take the QIHC someday and pass. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Siegrid.Pauwels <@t> uza.be Fri Aug 8 09:32:58 2014 From: Siegrid.Pauwels <@t> uza.be (Pauwels, Siegrid) Date: Fri Aug 8 09:33:07 2014 Subject: [Histonet] Please remove me from the list Message-ID: Pauwels Siegrid Hoofdlaborante/kwaliteitsfunctionaris Labo Pathologische anatomie U.Z.Antwerpen 03/8215593 siegrid.pauwels@uza.be From Charlotte.Kopczynski <@t> baycare.org Fri Aug 8 09:37:29 2014 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Fri Aug 8 09:37:37 2014 Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 12 In-Reply-To: <201408081429.s78ETbKx029540@pps.reinject> References: <201408081429.s78ETbKx029540@pps.reinject> Message-ID: <16323DA35673C448BA6824C1C12426E654E48866@BCEXMBX03.BCAD.BAYCARE.ORG> 44 years Thanks, Charlotte Kopczynski, HTL ASCP Regional Pathology Manager BayCare Laboratories Morton Plant Mease HealthCare Pathology Department 300 Pinellas Street Clearwater, Florida 33756 Phone: 727-461-8246 Fax: 727-462-7597 Cell: 727-580-1738 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, August 08, 2014 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Histonet Digest, Vol 129, Issue 11 (Sheryl McCandless) 2. RE: On the lighter side... (Ingles Claire) 3. Re: On the lighter side... (Eileen Akemi Allison) 4. RE: Bubble problems! (Melissa Ng) 5. Eons in Histology (nmhisto@comcast.net) 6. Correction (nmhisto@comcast.net) 7. RE: On the lighter side... (Podawiltz, Thomas) 8. Fw: [Histonet] On the lighter side... (Lee & Peggy Wenk) 9. RE: On the lighter side... (Blazek, Linda) 10. On the lighter side... (White, Lisa M.) 11. RE: On the lighter side... (Bernice Frederick) 12. Re: On the lighter side... (Jim Burchette) 13. Re: On the lighter side... (Rena Fail) 14. RE: On the lighter side... (Fischer, Dolores) 15. RE: On the lighter side... (Joelle Weaver) 16. RE: Correction (Weems, Joyce K.) 17. RE: On the lighter side... (O'Donnell, Bill) 18. RE: On the lighter side... (Mauger, Joanne) 19. Re: On the lighter side... (nmhisto@comcast.net) ---------------------------------------------------------------------- Message: 1 Date: Thu, 7 Aug 2014 17:21:25 -0500 From: "Sheryl McCandless" Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 11 To: Message-ID: <000901cfb28d$ed9520a0$c8bf61e0$@com> Content-Type: text/plain; charset="us-ascii" 10 years certified, 12 total! :) ------------------------------ Message: 2 Date: Thu, 7 Aug 2014 22:39:39 +0000 From: Ingles Claire Subject: RE: [Histonet] On the lighter side... To: undisclosed-recipients:; Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" 15 years and over 10 certified HTL. Claire Ingles ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Richard A. Carter [RCarter@Cancer-test.com] Sent: Thursday, August 07, 2014 4:36 PM To: 'Jennifer MacDonald'; Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 28 years Rick Carter -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Jennifer MacDonald Sent: Thursday, August 7, 2014 2:20 PM To: Douglas Porter Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 31 years Jennifer MacDonald From: "Douglas Porter" To: Date: 08/07/2014 11:36 AM Subject: [Histonet] On the lighter side... Sent by: histonet-bounces@lists.utsouthwestern.edu How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ------------------------------ Message: 3 Date: Thu, 7 Aug 2014 15:44:05 -0700 From: Eileen Akemi Allison Subject: Re: [Histonet] On the lighter side... To: Ingles Claire Cc: Histonet , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <5E4CAF81-76AD-40D5-AB99-9DF1D9BBA758@gmail.com> Content-Type: text/plain; charset=us-ascii 49 years and 45 years certified as HT and 22 as HTL Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Email: aallison@montereygi.com Tele: (831) 375-3577 X117 On Aug 7, 2014, at 3:39 PM, Ingles Claire wrote: > > 15 years and over 10 certified HTL. > Claire Ingles > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Richard A. Carter [RCarter@Cancer-test.com] > Sent: Thursday, August 07, 2014 4:36 PM > To: 'Jennifer MacDonald'; Douglas Porter > Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > 28 years > > Rick Carter > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Jennifer MacDonald > Sent: Thursday, August 7, 2014 2:20 PM > To: Douglas Porter > Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu > Subject: Re: [Histonet] On the lighter side... > > 31 years > > Jennifer MacDonald > > > > From: "Douglas Porter" > To: > Date: 08/07/2014 11:36 AM > Subject: [Histonet] On the lighter side... > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ------------------------------ Message: 4 Date: Fri, 8 Aug 2014 10:11:25 +0800 From: Melissa Ng Subject: [Histonet] RE: Bubble problems! To: "Horn, Hazel V" , 'Barbara Tibbs' , "Histonet@lists.utsouthwestern.edu" Message-ID: <39523B0CE90CC6458F92CAC795807902C238F718E2@vm023-exchange> Content-Type: text/plain; charset="us-ascii" Dear Hazel and Barbara, Thank you for your feedback. Wouldn't adding too much xylene result in drying effects post-coverslipping? Best thanks and regards, Melissa -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Thursday, 7 August, 2014 10:10 PM To: 'Barbara Tibbs'; Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: RE: Bubble problems! Melissa we are having the same problem. We use a different mounting medium. I will try Barbara's advice and try adding a bit of xylene to the media. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Barbara Tibbs Sent: Thursday, August 07, 2014 8:12 AM To: Melissa Ng; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Bubble problems! Try adding xylene to the mounting medium to thin it. About 10 ml of xylene to 90 ml of mounting medium should do it. Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Melissa Ng Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 5 Date: Fri, 8 Aug 2014 02:21:28 +0000 (UTC) From: nmhisto@comcast.net Subject: [Histonet] Eons in Histology To: HISTONET Message-ID: <831777913.1130973.1407464488206.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 I've been registered since 1969.?? This thread is interesting and I'd like to see what those of us Who Have Been in this Game so Long have as registry numbers.?? Mine is 04958 (I think...).?? How many of you can beat that??? But who's counting??? I retired 2.5 years ago but I'll always be a histotech! ------------------------------ Message: 6 Date: Fri, 8 Aug 2014 02:32:48 +0000 (UTC) From: nmhisto@comcast.net Subject: [Histonet] Correction To: HISTONET Message-ID: <2072541876.1133409.1407465168337.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 I must be getting old - my ASCP number is 04258, not 04958 (or whatever number I first wrote down)...?? I hate it when that happens! ------------------------------ Message: 7 Date: Fri, 8 Aug 2014 06:00:07 -0400 From: "Podawiltz, Thomas" Subject: RE: [Histonet] On the lighter side... To: Douglas Porter , "histonet@lists.utsouthwestern.edu" Message-ID: <38667E7FB77ECD4E91BFAEB8D9863863261589E632@LRGHEXVS1.practice.lrgh.org> Content-Type: text/plain; charset="us-ascii" 29 years. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 8 Date: Fri, 8 Aug 2014 07:21:59 -0400 From: "Lee & Peggy Wenk" Subject: Fw: [Histonet] On the lighter side... To: "Histonet" Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I have been thinking of unsubscribing to Histonet, but then something like this comes along and I start to rethink my stance. I've never been a histotech, but spent 33 glorious years listening to Peggy and others talk about it. Some went over my head, but as time went by, less and less. As you can tell from her posts, she loved any opportunity to teach. And Histonet provided a large opportunity. I'd like to take this opportunity myself to thank everybody for all their good thoughts and wishes. And take a moment of silence in Austin (how would that go) for Peggy. Thanks and with lots of love, Lee Wenk PS: I'll probably unsubscribe (the correct way) sometime in the not too distant future. -----Original Message----- From: Podawiltz, Thomas Sent: Friday, August 08, 2014 6:00 AM To: Douglas Porter ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 29 years. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ------------------------------ Message: 9 Date: Fri, 8 Aug 2014 07:28:15 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] On the lighter side... To: "histonet@lists.utsouthwestern.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E391692514DA1@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" 41 in histology 32 registered. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Podawiltz, Thomas Sent: Friday, August 08, 2014 6:00 AM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 29 years. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ------------------------------ Message: 10 Date: Fri, 8 Aug 2014 07:32:08 -0400 From: "White, Lisa M." Subject: [Histonet] On the lighter side... To: Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760C478197@VHAV09MSGA3.v09.med.va.gov> Content-Type: text/plain; charset="us-ascii" 15 years as HT...26 years total in medicine started as an EMT and after some time discovered that patients in a jar is a blessing J thus the transfer to Pathology......and that is the rest of the story. Lisa White, HT(ASCP) Classification: Internal and External Use\\Not VA Sensitive This message has been categorized by White, Lisa M. on Friday, August 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 ------------------------------ Message: 11 Date: Fri, 8 Aug 2014 12:11:13 +0000 From: Bernice Frederick Subject: RE: [Histonet] On the lighter side... To: Pam Barker , Douglas Porter , "'Baldridge, Lee Ann'" , "histonet@lists.utsouthwestern.edu" Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F2D41@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="utf-8" What's that in microns??? Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Pam Barker Sent: Thursday, August 07, 2014 3:11 PM To: Douglas Porter; 'Baldridge, Lee Ann'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I have been recruiting exclusively in histology for 10 years. ?? ; ) Thanks-Pam Right Place, Right Time, ??Right Opportunity.?? Pam Barker RELIA Solutions. relia1@earthlink.net (407) 353-5070 cell/text Sent from my Galaxy S??III
-------- Original message --------
From: Douglas Porter
Date:08/07/2014 4:10 PM (GMT-05:00)
To: "'Baldridge, Lee Ann'" , histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
Well...I appears most of us are right properly fixed. Good to see some "newbies" chiming in. This also shows that we need to get/keep more of the young ones interested in the field. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: Baldridge, Lee Ann [https://urldefense.proofpoint.com/v1/url?u=http://mailto:lhadley%40iupui.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=6561716ee4d85d6e9d879a86a14e3809bb27147aa8a1232e005023d489a7e199] Sent: Thursday, August 07, 2014 3:56 PM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 30years tomorrow. Lee Ann Baldridge IUSM -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4744 / Virus Database: 3986/7994 - Release Date: 08/06/14 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ------------------------------ Message: 12 Date: Fri, 8 Aug 2014 07:12:06 -0500 From: Jim Burchette Subject: Re: [Histonet] On the lighter side... To: "White, Lisa M." Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 40 blessed years that I would not trade for anything. I have met so many wonderful people (well, maybe a few not so wonderful...) and have learned so much. The good Lord has blessed me with a talent that I feel has allowed me to make a contribution to society. On Friday, August 8, 2014, White, Lisa M. wrote: > 15 years as HT...26 years total in medicine started as an EMT and after > some time discovered that patients in a jar is a blessing J thus the > transfer to Pathology......and that is the rest of the story. > > > > Lisa White, HT(ASCP) > > Classification: Internal and External Use\\Not VA Sensitive > This message has been categorized by White, Lisa M. on Friday, August > 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 > -- Jim Burchette "Fly Fishing Bum" *<'(((><* ------------------------------ Message: 13 Date: Fri, 8 Aug 2014 08:40:42 -0400 From: Rena Fail Subject: Re: [Histonet] On the lighter side... To: Jim Burchette Cc: "histonet@lists.utsouthwestern.edu" , "White, Lisa M." Message-ID: Content-Type: text/plain; charset=UTF-8 37 years On Friday, August 8, 2014, Jim Burchette wrote: > 40 blessed years that I would not trade for anything. I have met so many > wonderful people (well, maybe a few not so wonderful...) and have learned > so much. The good Lord has blessed me with a talent that I feel has allowed > me to make a contribution to society. > > On Friday, August 8, 2014, White, Lisa M. > wrote: > > > 15 years as HT...26 years total in medicine started as an EMT and after > > some time discovered that patients in a jar is a blessing J thus the > > transfer to Pathology......and that is the rest of the story. > > > > > > > > Lisa White, HT(ASCP) > > > > Classification: Internal and External Use\\Not VA Sensitive > > This message has been categorized by White, Lisa M. on Friday, August > > 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 > > > > > -- > Jim Burchette > "Fly Fishing Bum" *<'(((><* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 > ------------------------------ Message: 14 Date: Fri, 8 Aug 2014 12:52:09 +0000 From: "Fischer, Dolores" Subject: RE: [Histonet] On the lighter side... To: "histonet@lists.utsouthwestern.edu" Message-ID: <7CA324454CDDCA40A56FC3C51923C0E44B6E4AE6@BN1PRD9201MB008.026d.mgd.msft.net> Content-Type: text/plain; charset="utf-8" 39 good years. My HT cert # is 7435. The information transmitted is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: https://urldefense.proofpoint.com/v1/url?u=http://www.baxter.com/email_disclaimer&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=057253c32a119b0123fcd536eb058cbc96d315d19ae58343e40702b8277a81ed ------------------------------ Message: 15 Date: Fri, 8 Aug 2014 12:55:49 +0000 From: Joelle Weaver Subject: RE: [Histonet] On the lighter side... To: Rena Fail , Jim Burchette Cc: "histonet@lists.utsouthwestern.edu" , "White, Lisa M." Message-ID: Content-Type: text/plain; charset="iso-8859-1" Jim, you and many others who have devoted so much of your lives to this "craft" & profession, are appreciated by all of us who have learned and benefited from your knowledge and years of experience over the years. Thanks to ALL of you who have stuck in there the decades of time needed to gather all those experiences, and especially for being willing to share with us. It is people like yourself, and Peggy, and many others too numerous to name in an email, that keep the rest of us inspired to keep going when we get weary! Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Fri, 8 Aug 2014 08:40:42 -0400 > From: renafail2@gmail.com > To: jburch01@gmail.com > Subject: Re: [Histonet] On the lighter side... > CC: histonet@lists.utsouthwestern.edu; Lisa.White3@va.gov > > 37 years > > On Friday, August 8, 2014, Jim Burchette wrote: > > > 40 blessed years that I would not trade for anything. I have met so many > > wonderful people (well, maybe a few not so wonderful...) and have learned > > so much. The good Lord has blessed me with a talent that I feel has allowed > > me to make a contribution to society. > > > > On Friday, August 8, 2014, White, Lisa M. > > wrote: > > > > > 15 years as HT...26 years total in medicine started as an EMT and after > > > some time discovered that patients in a jar is a blessing J thus the > > > transfer to Pathology......and that is the rest of the story. > > > > > > > > > > > > Lisa White, HT(ASCP) > > > > > > Classification: Internal and External Use\\Not VA Sensitive > > > This message has been categorized by White, Lisa M. on Friday, August > > > 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 > > > > > > > > > -- > > Jim Burchette > > "Fly Fishing Bum" *<'(((><* > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ------------------------------ Message: 16 Date: Fri, 8 Aug 2014 12:55:44 +0000 From: "Weems, Joyce K." Subject: [Histonet] RE: Correction To: "'nmhisto@comcast.net'" , HISTONET Message-ID: Content-Type: text/plain; charset="utf-8" 03824 - HT 01653 - HTL Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph???s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of nmhisto@comcast.net Sent: Thursday, August 07, 2014 10:33 PM To: HISTONET Subject: [Histonet] Correction I must be getting old - my ASCP number is 04258, not 04958 (or whatever number I first wrote down)... I hate it when that happens! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 17 Date: Fri, 8 Aug 2014 13:20:49 +0000 From: "O'Donnell, Bill" Subject: RE: [Histonet] On the lighter side... To: Histonet , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" 30 years - Interesting demographics here, mainly (though not entirely) 'old timers' posting on this one. Logical conclusions: 1. It is a matter of pride or bragging rights that we geriatrics are posting or, 2. unnecessary shyness on the part of the younger members or, 3. the worst - very few younger followers of histonet Would love to see a whole bunch of newer techs posting - gives a great hope for the future of the profession -Bill This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 18 Date: Fri, 8 Aug 2014 14:00:32 +0000 From: "Mauger, Joanne" Subject: RE: [Histonet] On the lighter side... To: "Shirley A. Powell" , Paula Pierce , "histonet@lists.utsouthwestern.edu" Message-ID: <929548F049BC6D448DF9488B556449BE16562514@EXCMBXPW5.chop.edu> Content-Type: text/plain; charset="iso-8859-1" 35 long years!! Jo Mauger -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Shirley A. Powell Sent: Thursday, August 07, 2014 3:31 PM To: Paula Pierce; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 52 years, if we are all posting. Lol -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Paula Pierce Sent: Thursday, August 07, 2014 3:00 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 36 years. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Sullivan, Beatrice" To: 'Douglas Porter' ; "histonet@lists.utsouthwestern.edu" Sent: Thursday, August 7, 2014 1:51 PM Subject: RE: [Histonet] On the lighter side... 44 years here. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [https://urldefense.proofpoint.com/v1/url?u=http://mailto:histonet-bounces%40lists.utsouthwestern.edu&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=4755bd9631c53b24a88e7a983dbaaef300e69693820df26486bacfc2230153f7] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech?? 36 years here.? You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to ISSECURITY@virtua.org. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 ------------------------------ Message: 19 Date: Fri, 8 Aug 2014 14:28:20 +0000 (UTC) From: nmhisto@comcast.net Subject: Re: [Histonet] On the lighter side... To: Bill O'Donnell Cc: HISTONET , histonet-bounces@lists.utsouthwestern.edu Message-ID: <411800840.1336526.1407508100512.JavaMail.root@comcast.net> Content-Type: text/plain; charset=utf-8 I fear your last choice is the obvious conclusion.?? We need a prime-time medical drama focusing on laboratorians!?? We could create interest in histology if done correctly (no running a raw slide over a flame and placing it under the 'scope for diagnosis, for example). ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=Mb%2Ba6YWrITk%2FbDxaDjFExw%3D%3D%0A&r=HO7mYBe2LiMoewYfKRY31Yqs3%2FJq9QHICaj89zlnpBY%3D%0A&m=zAplvlxF1omxiNSmPsXMBmba7X8TEwgmOCuzqgam1zc%3D%0A&s=18b2141c2e6d0f8d063207b062ac2bb2405cfe56b34cb20722085a117c8b9bf5 End of Histonet Digest, Vol 129, Issue 12 ***************************************** Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From liz <@t> premierlab.com Fri Aug 8 09:53:52 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Aug 8 09:53:57 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org>, <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E021AB@SBS2K8.premierlab.local> Too funny Richard - you would pass with flying colors For me its 31 years - HTL-930 I have really been blessed, I love my job and I have really enjoyed my career. Every day there is something new to learn or to work on, for example the lab is putting the final touches on a poster that will be displayed at NSH this year, working on that has been exciting and a lot of fun too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard [Richard.Cartun@hhchealth.org] Sent: Friday, August 08, 2014 8:32 AM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... "36" years; however, not registered. Hopefully, I can take the QIHC someday and pass. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bbrinegarhtl <@t> gmail.com Fri Aug 8 10:00:46 2014 From: bbrinegarhtl <@t> gmail.com (Beth Brinegar) Date: Fri Aug 8 10:00:52 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E021AB@SBS2K8.premierlab.local> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> <14E2C6176416974295479C64A11CB9AE019C79E021AB@SBS2K8.premierlab.local> Message-ID: 3 years in histology, 2 years registered! Beth Brinegar HTL (ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala wrote: > Too funny Richard - you would pass with flying colors > > For me its 31 years - HTL-930 > > I have really been blessed, I love my job and I have really enjoyed my > career. Every day there is something new to learn or to work on, for > example the lab is putting the final touches on a poster that will be > displayed at NSH this year, working on that has been exciting and a lot of > fun too. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard [ > Richard.Cartun@hhchealth.org] > Sent: Friday, August 08, 2014 8:32 AM > To: Douglas Porter; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > "36" years; however, not registered. Hopefully, I can take the QIHC > someday and pass. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From algranth <@t> email.arizona.edu Fri Aug 8 10:32:58 2014 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Fri Aug 8 10:33:09 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> <14E2C6176416974295479C64A11CB9AE019C79E021AB@SBS2K8.premierlab.local>, Message-ID: I don't remember my number and I have no clue where that card is but I know it started with 09.... Got registered in 1979. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Beth Brinegar [bbrinegarhtl@gmail.com] Sent: Friday, August 08, 2014 8:00 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard Subject: Re: [Histonet] On the lighter side... 3 years in histology, 2 years registered! Beth Brinegar HTL (ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala wrote: > Too funny Richard - you would pass with flying colors > > For me its 31 years - HTL-930 > > I have really been blessed, I love my job and I have really enjoyed my > career. Every day there is something new to learn or to work on, for > example the lab is putting the final touches on a poster that will be > displayed at NSH this year, working on that has been exciting and a lot of > fun too. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard [ > Richard.Cartun@hhchealth.org] > Sent: Friday, August 08, 2014 8:32 AM > To: Douglas Porter; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > "36" years; however, not registered. Hopefully, I can take the QIHC > someday and pass. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Fri Aug 8 11:10:42 2014 From: b427297 <@t> aol.com (William J. O'Connor III) Date: Fri Aug 8 11:10:49 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <2B2ECF33934F5D4996D8BE03EFDF39760C478197@VHAV09MSGA3.v09.med.va.gov> Message-ID: <8D1813A7047DCF9-5B8-9925@webmail-vm035.sysops.aol.com> 44 years. HT(ASCP) 5483. Took a year off to run a gas station in the early 70's. Worked in Chicago, Memphis, Jacksonville, FL, San Diego, Honolulu. Lots of good times and great people. Jackie O' -----Original Message----- From: Rena Fail To: Jim Burchette Cc: histonet ; White, Lisa M. Sent: Fri, Aug 8, 2014 7:41 am Subject: Re: [Histonet] On the lighter side... 37 years On Friday, August 8, 2014, Jim Burchette wrote: > 40 blessed years that I would not trade for anything. I have met so many wonderful people (well, maybe a few not so wonderful...) and have learned so much. The good Lord has blessed me with a talent that I feel has allowed me to make a contribution to society. On Friday, August 8, 2014, White, Lisa M. > wrote: > 15 years as HT...26 years total in medicine started as an EMT and after > some time discovered that patients in a jar is a blessing J thus the > transfer to Pathology......and that is the rest of the story. > > > > Lisa White, HT(ASCP) > > Classification: Internal and External Use\\Not VA Sensitive > This message has been categorized by White, Lisa M. on Friday, August > 08, 2014 at 7:32:08 AM in accordance with VA Handbook 6500 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jim Burchette "Fly Fishing Bum" *<'(((><* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Aug 8 11:11:56 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Aug 8 11:12:05 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> Message-ID: Funny, Richard... as if you need registering. LOL Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, August 08, 2014 10:33 AM To: Douglas Porter; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... "36" years; however, not registered. Hopefully, I can take the QIHC someday and pass. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Joyce.Weems <@t> emoryhealthcare.org Fri Aug 8 11:13:05 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Aug 8 11:13:23 2014 Subject: [Histonet] RE: Please remove me from the list In-Reply-To: References: Message-ID: Hi there, You will need to remove yourself at http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pauwels, Siegrid Sent: Friday, August 08, 2014 10:33 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Please remove me from the list Pauwels Siegrid Hoofdlaborante/kwaliteitsfunctionaris Labo Pathologische anatomie U.Z.Antwerpen 03/8215593 siegrid.pauwels@uza.be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Timothy.Morken <@t> ucsfmedctr.org Fri Aug 8 11:45:03 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Aug 8 11:45:20 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> <14E2C6176416974295479C64A11CB9AE019C79E021AB@SBS2K8.premierlab.local> Message-ID: <761E2B5697F795489C8710BCC72141FF367917E6@ex07.net.ucsf.edu> Yah! A new one!!! Stay on Histonet, go to meetings and stay interested- the burnout prevention! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Friday, August 08, 2014 8:01 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard Subject: Re: [Histonet] On the lighter side... 3 years in histology, 2 years registered! Beth Brinegar HTL (ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala wrote: > Too funny Richard - you would pass with flying colors > > For me its 31 years - HTL-930 > > I have really been blessed, I love my job and I have really enjoyed my > career. Every day there is something new to learn or to work on, for > example the lab is putting the final touches on a poster that will be > displayed at NSH this year, working on that has been exciting and a > lot of fun too. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, > Richard [ Richard.Cartun@hhchealth.org] > Sent: Friday, August 08, 2014 8:32 AM > To: Douglas Porter; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > "36" years; however, not registered. Hopefully, I can take the QIHC > someday and pass. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution > is prohibited. If you are not the intended recipient, or an employee > or agent responsible for delivering the message to the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Fri Aug 8 12:10:58 2014 From: b427297 <@t> aol.com (William J. O'Connor III) Date: Fri Aug 8 12:11:11 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <761E2B5697F795489C8710BCC72141FF367917E6@ex07.net.ucsf.edu> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> <14E2C6176416974295479C64A11CB9AE019C79E021AB@SBS2K8.premierlab.local> <761E2B5697F795489C8710BCC72141FF367917E6@ex07.net.ucsf.edu> Message-ID: <8D18142DBA92D8D-2024-8EBC@webmail-vm055.sysops.aol.com> 44 years. HT(ASCP) #5483. Worked in Chicago, Memphis, Jacksonville, FL, San Diego, Honolulu, back to Chicago area now. Lots of fun, met many wondrous people along the way. Jackie O' From CIngles <@t> uwhealth.org Fri Aug 8 12:14:17 2014 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Aug 8 12:14:23 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <761E2B5697F795489C8710BCC72141FF367917E6@ex07.net.ucsf.edu> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> <14E2C6176416974295479C64A11CB9AE019C79E021AB@SBS2K8.premierlab.local> , <761E2B5697F795489C8710BCC72141FF367917E6@ex07.net.ucsf.edu> Message-ID: Just a foot note to all this. I still can't get my certification listed on my work badge because they've never heard of it. I'll just have to give them a tutorial...*sigh* I think this type of stuff is what gets me burned out the easiest. The thing that saves me are my co-workers. Tim is right about the meetings though. Besides you can also learn little tricks from the "More Experienced" among us. Teach me O Wise Ones. I am but forever a novice. :) Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Friday, August 08, 2014 11:45 AM To: 'Beth Brinegar'; Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard Subject: RE: [Histonet] On the lighter side... Yah! A new one!!! Stay on Histonet, go to meetings and stay interested- the burnout prevention! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA From jclark <@t> pcnm.com Fri Aug 8 12:28:07 2014 From: jclark <@t> pcnm.com (Joanne Clark) Date: Fri Aug 8 12:28:15 2014 Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 14 In-Reply-To: <20140808170203.BDC75A7DED4@mx10.myoutlookonline.com> References: <20140808170203.BDC75A7DED4@mx10.myoutlookonline.com> Message-ID: <0494A7D4E8CC254EA2FB81464982E378B4BC2B5A@S10MAILD001N4.SH10.lan> I have been a histotech for 28 years. The first 20 with my MLT (Canadian certification)and the last 8 here in beautiful and warm New Mexico with my HT. Completed my Bachelor's last year, so hopefully I can get my HTL within the next year or two. Joanne Clark, BAAS, HT Director of Histology Pathology Consultants of New Mexico Message: 2 Date: Fri, 8 Aug 2014 16:45:03 +0000 From: "Morken, Timothy" Subject: RE: [Histonet] On the lighter side... To: "'Beth Brinegar'" , "Elizabeth Chlipala" Cc: "histonet@lists.utsouthwestern.edu" , "Cartun, Richard" Message-ID: <761E2B5697F795489C8710BCC72141FF367917E6@ex07.net.ucsf.edu> Content-Type: text/plain; charset=utf-8 Yah! A new one!!! Stay on Histonet, go to meetings and stay interested- the burnout prevention! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Friday, August 08, 2014 8:01 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard Subject: Re: [Histonet] On the lighter side... 3 years in histology, 2 years registered! Beth Brinegar HTL (ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala wrote: > Too funny Richard - you would pass with flying colors > > For me its 31 years - HTL-930 > > I have really been blessed, I love my job and I have really enjoyed my > career. Every day there is something new to learn or to work on, for > example the lab is putting the final touches on a poster that will be > displayed at NSH this year, working on that has been exciting and a > lot of fun too. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, > Richard [ Richard.Cartun@hhchealth.org] > Sent: Friday, August 08, 2014 8:32 AM > To: Douglas Porter; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > "36" years; however, not registered. Hopefully, I can take the QIHC > someday and pass. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution > is prohibited. If you are not the intended recipient, or an employee > or agent responsible for delivering the message to the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 14 ***************************************** Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From christina.wolfe <@t> bms.com Fri Aug 8 12:59:00 2014 From: christina.wolfe <@t> bms.com (Wolfe, Christina) Date: Fri Aug 8 12:59:09 2014 Subject: [Histonet] RE: Bubble problems In-Reply-To: <2301a3cb-fbf7-47bc-9460-5875baa6224f@ushpwbmsmhp003.one.ads.bms.com> References: <2301a3cb-fbf7-47bc-9460-5875baa6224f@ushpwbmsmhp003.one.ads.bms.com> Message-ID: Hi Melissa, We have intermittently had problems with our Leica Glass coverslipper over the years. During troubleshooting with their tech support, we found our issue was most likely with the coverslips. We use the VWR micro glass 24x50 mm cat# 48404-452 coverslips and the Tissue-Tek Glas mounting medium cat #6419. There are a couple of settings on the instrument that control the amount of mounting medium flow onto the glass slide - you can call me if you want our settings that work well for us. Also, the xylene in the 'holding area' on the coverslipper should be changed regularly and needs to be pretty full (there is a line on ours). We have found if that xylene is too old and if the volume of xylene drops below the fill line - we start getting micro bubbles. One last thing, it is important to also push the button to drop the mounting medium dispenser at the end of the day to be submerged in the little container of xylene and to make sure and clean the rubber stopper that picks up the glass coverslips with the brush before each day of use. We have found that all of these elements affect air bubbles on the slides. Feel free to give me a call if you like. Christina Wolfe Bristol Myers Squibb 812-307-2093 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Melissa Ng Sent: Thursday, August 07, 2014 7:59 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Bubble problems! Dear Histonet, I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe! Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us! The Cellpath mounting media is way more viscous compared to that of the Leica media. Has anyone here experienced a similar issue, and how have you resolved it? Appreciate all the help I can get! Best regards, Melissa Application Specialist _______________________________________________ ****** This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From POWELL_SA <@t> mercer.edu Fri Aug 8 13:05:02 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Aug 8 13:05:08 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <761E2B5697F795489C8710BCC72141FF367917E6@ex07.net.ucsf.edu> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> <14E2C6176416974295479C64A11CB9AE019C79E021AB@SBS2K8.premierlab.local> <761E2B5697F795489C8710BCC72141FF367917E6@ex07.net.ucsf.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BFB25F7FB@MERCERMAIL.MercerU.local> Yes, what Tim said, stay active. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, August 08, 2014 12:45 PM To: 'Beth Brinegar'; Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard Subject: RE: [Histonet] On the lighter side... Yah! A new one!!! Stay on Histonet, go to meetings and stay interested- the burnout prevention! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Friday, August 08, 2014 8:01 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard Subject: Re: [Histonet] On the lighter side... 3 years in histology, 2 years registered! Beth Brinegar HTL (ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala wrote: > Too funny Richard - you would pass with flying colors > > For me its 31 years - HTL-930 > > I have really been blessed, I love my job and I have really enjoyed my > career. Every day there is something new to learn or to work on, for > example the lab is putting the final touches on a poster that will be > displayed at NSH this year, working on that has been exciting and a > lot of fun too. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, > Richard [ Richard.Cartun@hhchealth.org] > Sent: Friday, August 08, 2014 8:32 AM > To: Douglas Porter; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > "36" years; however, not registered. Hopefully, I can take the QIHC > someday and pass. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution > is prohibited. If you are not the intended recipient, or an employee > or agent responsible for delivering the message to the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Fri Aug 8 13:26:11 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Aug 8 13:26:19 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 1992. Electron Microscopy Technologist, #604, 1982. Like most, I never heard of "histology" until I walked into a hospital lab on my first day as an EM tech. I had seen slides made in college, but no one ever mentioned it could be an actual profession. I was more taken with the electron microscope, and there was (is) a 2-year program at the community college in the town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a marketable skill. At that time EM was still used for tumor dx, so when I started it was about half tumor, half kidney. I was lucky enough to get involved in histology and set up the IHC lab at the small community hospital I worked at (as an EM tech) and so ended up phasing myself almost out of an EM job. The IHC took over all the tumor dx from EM. Later I left EM altogether and did IHC exclusively for 15 years. But, like most, I learned Histotechnology on the job but was lucky enough to work for a pathologist who believed in developing his techs - to the point of paying for meetings out of his own pocket. Only now do I know how fortunate I was to work for someone like that. Because of him we had developed a culture in the small histo lab (4 men!!) of learning. We studied together one night a week for the HT exam and all passed (and the practical!). Again, that was a fortunate experience, not very often seen in labs. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drbugge <@t> gmail.com Fri Aug 8 13:40:34 2014 From: drbugge <@t> gmail.com (Dawn Bugge) Date: Fri Aug 8 13:40:39 2014 Subject: [Histonet] Are Paraffin Blocks Biohazard Message-ID: Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website From wbenton <@t> cua.md Fri Aug 8 13:52:18 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Fri Aug 8 13:54:26 2014 Subject: [Histonet] Are Paraffin Blocks Biohazard In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD93938636AA3@CUAEXH1.GCU-MD.local> Dawn, Take a look at this document. It should help you. IATA and DOT have plenty of documents and training that you can take to understand this better. http://www.ncsu.edu/ehs/dot/Bio_shipping.pdf Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge [drbugge@gmail.com] Sent: Friday, August 08, 2014 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Paraffin Blocks Biohazard Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From hist10 <@t> aol.com Fri Aug 8 13:54:58 2014 From: hist10 <@t> aol.com (Roslyn Macon) Date: Fri Aug 8 13:55:08 2014 Subject: Fwd: [Histonet] On the lighter side... References: Message-ID: <6E5F1410-3D46-439C-82E9-5B75AF8B39B2@aol.com> 44year--retirement two week ago, love the job and the work for all 44 year. Lab super. For 33 years. Sent from my iPhone Begin forwarded message: > From: "Connolly, Brett M" > Date: August 7, 2014 4:24:53 PM EDT > To: "histonet@lists.utsouthwestern.edu" > Subject: RE: [Histonet] On the lighter side... > > 34 years for me, but it seems like just yesterday it was 33.999 years. > > Brett M. Connolly, Ph.D. > Principle Scientist, Imaging Dept. > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly@merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Aug 8 13:59:43 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Aug 8 13:59:49 2014 Subject: [Histonet] Are Paraffin Blocks Biohazard In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD4BB@SBS2K8.premierlab.local> Dawn I think it may be on the OSHA website under the bloodborne pathogen standard - fixed tissue is considered non-infectious and non-hazardous. Below is what OSHA considers potentially infectious materials and specifically addressed unfixed tissue, fixed tissue would therefore be considered non-infectious. occupational exposure to blood or other potentially infectious materials (OPIM) place workers at risk for infection with bloodborne pathogens. OSHA defines blood to mean human blood, human blood components, and products made from human blood. Other potentially infectious materials (OPIM) means: (1) The following human body fluids: semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any body fluid that is visibly contaminated with blood, and all body fluids in situations where it is difficult or impossible to differentiate between body fluids; (2) Any unfixed tissue or organ (other than intact skin) from a human (living or dead); and (3) HIV-containing cell or tissue cultures, organ cultures, and HIV- or HBV-containing culture medium or other solutions; and blood, organs, or other tissues from experimental animals infected with HIV or HBV. The following references aid in recognizing workplace hazards associated with bloodborne pathogens. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Friday, August 08, 2014 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Paraffin Blocks Biohazard Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Aug 8 14:05:59 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Aug 8 14:06:04 2014 Subject: [Histonet] Are Paraffin Blocks Biohazard In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECD4BB@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECD4BB@SBS2K8.premierlab.local> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F2F18@evcspmbx2.ads.northwestern.edu> Same for IATA (shipping )We have to take the training every 3 years... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, August 08, 2014 2:00 PM To: Dawn Bugge; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Are Paraffin Blocks Biohazard Dawn I think it may be on the OSHA website under the bloodborne pathogen standard - fixed tissue is considered non-infectious and non-hazardous. Below is what OSHA considers potentially infectious materials and specifically addressed unfixed tissue, fixed tissue would therefore be considered non-infectious. occupational exposure to blood or other potentially infectious materials (OPIM) place workers at risk for infection with bloodborne pathogens. OSHA defines blood to mean human blood, human blood components, and products made from human blood. Other potentially infectious materials (OPIM) means: (1) The following human body fluids: semen, vaginal secretions, cerebrospinal fluid, synovial fluid, pleural fluid, pericardial fluid, peritoneal fluid, amniotic fluid, saliva in dental procedures, any body fluid that is visibly contaminated with blood, and all body fluids in situations where it is difficult or impossible to differentiate between body fluids; (2) Any unfixed tissue or organ (other than intact skin) from a human (living or dead); and (3) HIV-containing cell or tissue cultures, organ cultures, and HIV- or HBV-containing culture medium or other solutions; and blood, organs, or other tissues from experimental animals infected with HIV or HBV. The following references aid in recognizing workplace hazards associated with bloodborne pathogens. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Friday, August 08, 2014 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Paraffin Blocks Biohazard Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From claycal44 <@t> yahoo.com Fri Aug 8 15:36:12 2014 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Fri Aug 8 15:36:17 2014 Subject: [Histonet] Water/Bubbles In-Reply-To: References: Message-ID: <1407530172.88741.YahooMailNeo@web125604.mail.ne1.yahoo.com> I have also had this problem recently. Nancy Lowen On Thursday, August 7, 2014 1:23 PM, Julie Bowman wrote: Hello all, Recently we have been experiencing a problem when running H&E slides on our Gemini stainer (Thermo Shandon Varistain). At the end of the run, small water droplets or possibly air bubbles are seen on the slides after taking the slides out of xylene. If they are coverslipped, a white haze will be seen around the specimen after a few minutes. Any suggestions of what causes this and how to correct it? Thank you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis <@t> seattlechildrens.org Fri Aug 8 17:05:50 2014 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Fri Aug 8 17:05:57 2014 Subject: [Histonet] Does anyone know where I can get a metal container to hold isopentane that I would place my OCT molds into when making Frozen blocks? Message-ID: <3903BE18914F4440834F0E620415FFCA3CB1BC03@PPWEXD01a.childrens.sea.kids> Hi Everyone. I make my Frozen blocks by Immersing the cryo molds in a metal container (Looks like a larger flat base ? coke can) filled with Isopentane solution. That container is then surrounded by Dry Ice. We need a new metal container. Does anyone know where I could buy one? I don't really want to change to an Aluminum block method as I like the penetration freezing I get from liquid emersion. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken <@t> ucsfmedctr.org Fri Aug 8 17:11:19 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Fri Aug 8 17:11:32 2014 Subject: [Histonet] Does anyone know where I can get a metal container to hold isopentane that I would place my OCT molds into when making Frozen blocks? In-Reply-To: <3903BE18914F4440834F0E620415FFCA3CB1BC03@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA3CB1BC03@PPWEXD01a.childrens.sea.kids> Message-ID: <761E2B5697F795489C8710BCC72141FF367919C9@ex07.net.ucsf.edu> Patrick, Look up "steel beakers" on the Fisher website (fishersci.com) and you'll see a variety. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Friday, August 08, 2014 3:06 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Does anyone know where I can get a metal container to hold isopentane that I would place my OCT molds into when making Frozen blocks? Hi Everyone. I make my Frozen blocks by Immersing the cryo molds in a metal container (Looks like a larger flat base ? coke can) filled with Isopentane solution. That container is then surrounded by Dry Ice. We need a new metal container. Does anyone know where I could buy one? I don't really want to change to an Aluminum block method as I like the penetration freezing I get from liquid emersion. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Sat Aug 9 04:44:02 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Sat Aug 9 04:41:35 2014 Subject: [Histonet] Water/Bubbles In-Reply-To: References: Message-ID: <001a01cfb3b6$752b8580$5f829080$@rowaihi@alborglaboratories.com> Make sure of the purity of the Abs. Alcohol in dehydration steps. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julie Bowman Sent: Thursday, August 07, 2014 11:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water/Bubbles Hello all, Recently we have been experiencing a problem when running H&E slides on our Gemini stainer (Thermo Shandon Varistain). At the end of the run, small water droplets or possibly air bubbles are seen on the slides after taking the slides out of xylene. If they are coverslipped, a white haze will be seen around the specimen after a few minutes. Any suggestions of what causes this and how to correct it? Thank you! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Sat Aug 9 04:47:34 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Sat Aug 9 04:45:07 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <3D4A471B82E7A44C87F6839732320D9F013FC79FA3@VSPDMS-ITEXMB02.DMS.COM> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <3D4A471B82E7A44C87F6839732320D9F013FC79FA3@VSPDMS-ITEXMB02.DMS.COM> Message-ID: <001b01cfb3b6$f3f60480$dbe20d80$@rowaihi@alborglaboratories.com> Similar as me So strong, proud with high confidence Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vincent Rivera Sent: Thursday, August 07, 2014 11:58 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 15 Years and still going strong :) Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Aug 9 10:07:42 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Aug 9 10:07:46 2014 Subject: [Histonet] Are Paraffin Blocks Biohazard In-Reply-To: References: Message-ID: <1407596862.86306.YahooMailNeo@web120401.mail.ne1.yahoo.com> Tell your pathologist to relax, nobody in the lab will sue him (or her) for any disease gotten from a paraffin block OTHER than a prion case. As far as I know nobody has wasted time and money in proving this issue but you can have "a hint" when the "ultra cautious" CAP has not classify them as "infectious". Ren? J.? On Friday, August 8, 2014 2:41 PM, Dawn Bugge wrote: Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard.? I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this?? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 11z <@t> comcast.net Sun Aug 10 15:27:58 2014 From: 11z <@t> comcast.net (LeRoy Brown) Date: Sun Aug 10 15:28:20 2014 Subject: [Histonet] looking for used unwanted Tissue-tek paraffin block files Message-ID: <00ae01cfb4d9$934b9220$b9e2b660$@comcast.net> Hi. I need to find some used tissue-tek block file cabinets. Can you contact me if you have some I could purchase or take off your hands?? Contact Roy at 11z@comcast.net FYI: I would be using these in my lab and not planning on selling them. If you know of someone who may be selling used files let me know too, I cannot afford new ones. They just cost way too much. Roy Brown HT(ASCP) HTL From LSebree <@t> uwhealth.org Sun Aug 10 16:50:58 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Sun Aug 10 16:51:06 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <001b01cfb3b6$f3f60480$dbe20d80$@rowaihi@alborglaboratories.com> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <3D4A471B82E7A44C87F6839732320D9F013FC79FA3@VSPDMS-ITEXMB02.DMS.COM>, <001b01cfb3b6$f3f60480$dbe20d80$@rowaihi@alborglaboratories.com> Message-ID: <77DD817201982748BC67D7960F2F76AF0C7C6D@UWHC-MBX12.uwhis.hosp.wisc.edu> 38 years and looking forward to retirement in a few years. Linda A. Sebree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Jamal [j.rowaihi@alborglaboratories.com] Sent: Saturday, August 09, 2014 4:47 AM To: 'Vincent Rivera'; 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Similar as me So strong, proud with high confidence Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vincent Rivera Sent: Thursday, August 07, 2014 11:58 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 15 Years and still going strong :) Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Mon Aug 11 01:58:03 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Mon Aug 11 01:58:13 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <9215BD4B0BA1B44D962A71C758B68D2E355C394C@HHCEXCHMB03.hhcsystem.org> <14E2C6176416974295479C64A11CB9AE019C79E021AB@SBS2K8.premierlab.local> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FBFED1D42@FWDCWPMSGCMS09.hca.corpad.net> For me 36years..will not be 37, I retire in 3 weeks!!!!! Off to visit my son and family in England! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Friday, August 08, 2014 11:01 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard Subject: Re: [Histonet] On the lighter side... 3 years in histology, 2 years registered! Beth Brinegar HTL (ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala wrote: > Too funny Richard - you would pass with flying colors > > For me its 31 years - HTL-930 > > I have really been blessed, I love my job and I have really enjoyed my > career. Every day there is something new to learn or to work on, for > example the lab is putting the final touches on a poster that will be > displayed at NSH this year, working on that has been exciting and a > lot of fun too. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, > Richard [ Richard.Cartun@hhchealth.org] > Sent: Friday, August 08, 2014 8:32 AM > To: Douglas Porter; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > "36" years; however, not registered. Hopefully, I can take the QIHC > someday and pass. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution > is prohibited. If you are not the intended recipient, or an employee > or agent responsible for delivering the message to the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Mon Aug 11 05:46:33 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Mon Aug 11 05:44:04 2014 Subject: [Histonet] Cytospin validation Message-ID: <011101cfb551$85e59db0$91b0d910$@rowaihi@alborglaboratories.com> Hi I need to do validation for my Shandon Cytospin 4, can anyone send me the validation procedure and form. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From ree3 <@t> leicester.ac.uk Mon Aug 11 06:17:49 2014 From: ree3 <@t> leicester.ac.uk (Edwards, Richard) Date: Mon Aug 11 06:17:57 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> Message-ID: Sniffed my first formalin and saw first post-mortem November 1965. Richard Edwards Leicester U.K. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: 08 August 2014 19:26 To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 1992. Electron Microscopy Technologist, #604, 1982. Like most, I never heard of "histology" until I walked into a hospital lab on my first day as an EM tech. I had seen slides made in college, but no one ever mentioned it could be an actual profession. I was more taken with the electron microscope, and there was (is) a 2-year program at the community college in the town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a marketable skill. At that time EM was still used for tumor dx, so when I started it was about half tumor, half kidney. I was lucky enough to get involved in histology and set up the IHC lab at the small community hospital I worked at (as an EM tech) and so ended up phasing myself almost out of an EM job. The IHC took over all the tumor dx from EM. Later I left EM altogether and did IHC exclusively for 15 years. But, like most, I learned Histotechnology on the job but was lucky enough to work for a pathologist who believed in developing his techs - to the point of paying for meetings out of his own pocket. Only now do I know how fortunate I was to work for someone like that. Because of him we had developed a culture in the small histo lab (4 men!!) of learning. We studied together one night a week for the HT exam and all passed (and the practical!). Again, that was a fortunate experience, not very often seen in labs. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sforeman <@t> labpath.com Mon Aug 11 07:37:21 2014 From: sforeman <@t> labpath.com (Susan Foreman) Date: Mon Aug 11 07:45:26 2014 Subject: [Histonet] Laboratory Refrigerator w glass doors Message-ID: <003601cfb561$0005f7c0$0011e740$@com> We are looking at a new laboratory refrigerator >23ft for our IHC department. What models would you recommend and which to avoid? It's a Monday! Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 From mjones <@t> metropath.com Mon Aug 11 09:07:28 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Aug 11 09:07:39 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> Message-ID: 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > > > > Richard Edwards > > > Leicester U.K. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, >Timothy >Sent: 08 August 2014 19:26 >To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > >Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL >1369, 1992. Electron Microscopy Technologist, #604, 1982. > >Like most, I never heard of "histology" until I walked into a hospital >lab on my first day as an EM tech. I had seen slides made in college, >but no one ever mentioned it could be an actual profession. I was more >taken with the electron microscope, and there was (is) a 2-year program >at the community college in the town I grew up in (Delta College, >Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a >marketable skill. At that time EM was still used for tumor dx, so when I >started it was about half tumor, half kidney. I was lucky enough to get >involved in histology and set up the IHC lab at the small community >hospital I worked at (as an EM tech) and so ended up phasing myself >almost out of an EM job. The IHC took over all the tumor dx from EM. >Later I left EM altogether and did IHC exclusively for 15 years. But, >like most, I learned Histotechnology on the job but was lucky enough to >work for a pathologist who believed in developing his techs - to the >point of paying for meetings out of his own pocket. Only now do I know >how fortunate I was to work for someone like that. Because of him we had >developed a culture in the small histo lab (4 men!!) of learning. We >studied together one night a week for the HT exam and all passed (and the >practical!). Again, that was a fortunate experience, not very often seen >in labs. > >Tim Morken >Supervisor, Histology, Electron Microscopy and Neuromuscular Special >Studies UC San Francisco Medical Center San Francisco, CA > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 11:39 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech? 36 years here. You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LJohns53 <@t> its.jnj.com Mon Aug 11 10:44:53 2014 From: LJohns53 <@t> its.jnj.com (Johns, Laura [ETHUS]) Date: Mon Aug 11 10:45:06 2014 Subject: [Histonet] Anti MMP-9 Message-ID: <0295CDAF7914EF4D810D7C5541F7CAE224A1228F@ITSUSRAGMDGC06.jnj.com> Hello, Can anyone recommend an antibody for MMP-9 that works in FFPE porcine tissue? Thanks, Laura From CIngles <@t> uwhealth.org Mon Aug 11 10:59:53 2014 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Aug 11 11:00:01 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> , Message-ID: Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > From mjones <@t> metropath.com Mon Aug 11 11:21:55 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Aug 11 11:22:08 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <62C639732D3F274DACED033EBDF6ADAF2F0F311E@evcspmbx2.ads.northwestern.edu> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> <62C639732D3F274DACED033EBDF6ADAF2F0F311E@evcspmbx2.ads.northwestern.edu> Message-ID: Oh, I ment to say ?Lots of tenure here and lots of brain cells here? meaning this website and all histotechs here, not myself. Obviously I don?t have many brain cells left If I made it look like I was talking about myself. . .sheesh. . Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 10:18 AM, "Bernice Frederick" wrote: >It was Friday,I was getting goofy doing paperwork,....... > >Bernice Frederick HTL (ASCP) >Senior Research Tech >Pathology Core Facility >ECOGPCO-RL >Robert. H. Lurie Cancer Center >Northwestern University >710 N Fairbanks Court >Olson 8-421 >Chicago,IL 60611 >312-503-3723 >b-frederick@northwestern.edu > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael >Ann Jones >Sent: Monday, August 11, 2014 9:07 AM >To: Edwards, Richard; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >25 years, ("what?s that in micron?s??) Bernice, you are too funny!! >(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) >Histology Manager Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: > >>Sniffed my first formalin and saw first post-mortem November 1965. >> >> >> >> Richard Edwards >> >> >> Leicester U.K. >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, >>Timothy >>Sent: 08 August 2014 19:26 >>To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu >>Subject: RE: [Histonet] On the lighter side... >> >>Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL >>1369, 1992. Electron Microscopy Technologist, #604, 1982. >> >>Like most, I never heard of "histology" until I walked into a hospital >>lab on my first day as an EM tech. I had seen slides made in college, >>but no one ever mentioned it could be an actual profession. I was more >>taken with the electron microscope, and there was (is) a 2-year program >>at the community college in the town I grew up in (Delta College, >>Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a >>marketable skill. At that time EM was still used for tumor dx, so when >>I started it was about half tumor, half kidney. I was lucky enough to >>get involved in histology and set up the IHC lab at the small community >>hospital I worked at (as an EM tech) and so ended up phasing myself >>almost out of an EM job. The IHC took over all the tumor dx from EM. >>Later I left EM altogether and did IHC exclusively for 15 years. But, >>like most, I learned Histotechnology on the job but was lucky enough to >>work for a pathologist who believed in developing his techs - to the >>point of paying for meetings out of his own pocket. Only now do I know >>how fortunate I was to work for someone like that. Because of him we >>had developed a culture in the small histo lab (4 men!!) of learning. >>We studied together one night a week for the HT exam and all passed >>(and the practical!). Again, that was a fortunate experience, not very >>often seen in labs. >> >>Tim Morken >>Supervisor, Histology, Electron Microscopy and Neuromuscular Special >>Studies UC San Francisco Medical Center San Francisco, CA >> >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >>Porter >>Sent: Thursday, August 07, 2014 11:39 AM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] On the lighter side... >> >>How long have you been a registered histotech? 36 years here. You??? >> >> >> >>Douglas A. Porter, HT (ASCP) >>Grossing Technician >>IT Coordinator >> >>Cancer Registrar >> >> >>CAP-Lab, PLC >>2508 South Cedar Street >>Lansing, MI 48910-3138 >> >>517-372-5520 (phone) >>517-372-5540 (fax) >> >> doug.porter@caplab.org >> >> www.caplab.org >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Fawn.Bomar <@t> HalifaxRegional.com Mon Aug 11 11:47:43 2014 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Mon Aug 11 11:48:20 2014 Subject: [Histonet] Ventana Symphony Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B9745C7A@EXCH-2K10.hrhs.com> Can anyone using the Ventana Symphony share its pros and cons with me? Would you recommend this stainer? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From hans <@t> histologistics.com Mon Aug 11 11:51:05 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Mon Aug 11 11:51:10 2014 Subject: [Histonet] Need microvascular (MVD) staining and quantification of tumro vasculature. Message-ID: Hello All, I have a customer who needs microvascular (MVD) staining and quantification of tumro vasculature, where staining is typically done using CD31. Is there anyone who can do this and willing to forward their information to me? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From POWELL_SA <@t> mercer.edu Mon Aug 11 12:03:38 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Mon Aug 11 12:03:54 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> , Message-ID: <9BF995BC0E47744E9673A41486E24EE25BFB25FD10@MERCERMAIL.MercerU.local> I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From heather.l.knight3 <@t> gmail.com Mon Aug 11 12:18:57 2014 From: heather.l.knight3 <@t> gmail.com (Heather Knight) Date: Mon Aug 11 12:19:02 2014 Subject: [Histonet] Bcl-2 Message-ID: Hi everyone- Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse tissue? If so, can you share both the protocol and the antibody information? We have tried numerous antibodies over the years with very limited success. Thank you for your help!! Best, Heather Knight From craigak12 <@t> gmail.com Mon Aug 11 12:18:53 2014 From: craigak12 <@t> gmail.com (Jb) Date: Mon Aug 11 12:19:05 2014 Subject: [Histonet] IHC Steam Strips: Message-ID: <375F4EF9-47B6-46CD-B5B1-B511309EF4AA@gmail.com> Does anyone use IHC steam strips in their decloaking chamber? The question is do you run a new steam strip each run and log each individual run w/it's own steam strip? Thank you, Sent from my iPhone From Michael.LaFriniere <@t> ccplab.com Mon Aug 11 12:43:34 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Mon Aug 11 12:43:47 2014 Subject: [Histonet] Are Paraffin Blocks Biohazard In-Reply-To: References: Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D296A0A37@AHCMSASEXCH00.my.ahc.local> Dawn, The only study I know of is on CJD crutsfeldt-Jacobs Disease (know to survive formalin fixation and routine processing protocols, the CDC web site has additional information, In my laboratories I put all blocks in hazardous waste for incineration disposal. It is not that costly just to be on the safe side. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Friday, August 08, 2014 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Paraffin Blocks Biohazard Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mturner <@t> CSILaboratories.com Mon Aug 11 12:49:13 2014 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Mon Aug 11 12:49:34 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25BFB25FD10@MERCERMAIL.MercerU.local> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> , <9BF995BC0E47744E9673A41486E24EE25BFB25FD10@MERCERMAIL.MercerU.local> Message-ID: <643626B74DE2814D8537057F40E1A10B0CAD409D@CSI-MX-NODEA.CSI-LABS.local> Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Aug 11 12:53:31 2014 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 11 12:53:36 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <643626B74DE2814D8537057F40E1A10B0CAD409D@CSI-MX-NODEA.CSI-LABS.local> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> , <9BF995BC0E47744E9673A41486E24EE25BFB25FD10@MERCERMAIL.MercerU.local> <643626B74DE2814D8537057F40E1A10B0CAD409D@CSI-MX-NODEA.CSI-LABS.local> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F3167@evcspmbx2.ads.northwestern.edu> Or we'll live forever..... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Monday, August 11, 2014 12:49 PM To: Shirley A. Powell; Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WaitT <@t> livemail.uthscsa.edu Mon Aug 11 14:26:57 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Mon Aug 11 14:27:23 2014 Subject: [Histonet] Weight Loss/Weight Gain Decal Message-ID: <1407785218488.59806@livemail.uthscsa.edu> Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry From JMacDonald <@t> mtsac.edu Mon Aug 11 14:33:51 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Aug 11 14:33:56 2014 Subject: [Histonet] Weight Loss/Weight Gain Decal In-Reply-To: <1407785218488.59806@livemail.uthscsa.edu> References: <1407785218488.59806@livemail.uthscsa.edu> Message-ID: I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" To: "histonet@lists.utsouthwestern.edu" Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces@lists.utsouthwestern.edu Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WaitT <@t> livemail.uthscsa.edu Mon Aug 11 14:35:29 2014 From: WaitT <@t> livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Mon Aug 11 14:35:40 2014 Subject: [Histonet] Weight Loss/Weight Gain Decal In-Reply-To: References: <1407785218488.59806@livemail.uthscsa.edu>, Message-ID: <1407785730771.24250@livemail.uthscsa.edu> Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" To: "histonet@lists.utsouthwestern.edu" Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces@lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Mon Aug 11 14:45:01 2014 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Aug 11 14:46:10 2014 Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 17 In-Reply-To: References: Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B4382A0@D1PWPEXMBX05.mdanderson.edu> Egads, I feel like a baby! 20 years registered, 3 yrs unregistered. By the way, I did see a former colleague with her ASCP certificate in her office, and the oldest sticker is from the year I was born. Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 2 Date: Sun, 10 Aug 2014 21:50:58 +0000 From: Sebree Linda A Subject: RE: [Histonet] On the lighter side... To: Jamal , 'Vincent Rivera' , 'Douglas Porter' , "histonet@lists.utsouthwestern.edu" Message-ID: <77DD817201982748BC67D7960F2F76AF0C7C6D@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" 38 years and looking forward to retirement in a few years. Linda A. Sebree From TNMayer <@t> mdanderson.org Mon Aug 11 14:52:19 2014 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Mon Aug 11 14:52:45 2014 Subject: [Histonet] RE:On the lighter side... Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B4382E6@D1PWPEXMBX05.mdanderson.edu> Tim, Just saw your post about the 'marketable skill'. Funny those were my mother's exact words while I was in college. She didn't care what I majored in, as long as I got a marketable skill along the way. The best advice I've ever gotten. Thanks Maria!!! (my mother) Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: 08 August 2014 19:26 To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 1992. Electron Microscopy Technologist, #604, 1982. Like most, I never heard of "histology" until I walked into a hospital lab on my first day as an EM tech. I had seen slides made in college, but no one ever mentioned it could be an actual profession. I was more taken with the electron microscope, and there was (is) a 2-year program at the community college in the town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a marketable skill. At that time EM was still used for tumor dx, so when I started it was about half tumor, half kidney. I was lucky enough to get involved in histology and set up the IHC lab at the small community hospital I worked at (as an EM tech) and so ended up phasing myself almost out of an EM job. The IHC took over all the tumor dx from EM. Later I left EM altogether and did IHC exclusively for 15 years. But, like most, I learned Histotechnology on the job but was lucky enough to work for a pathologist who believed in developing his techs - to the point of paying for meetings out of his own pocket. Only now do I know how fortunate I was to work for someone like that. Because of him we had developed a culture in the small histo lab (4 men!!) of learning. We studied together one night a week for the HT exam and all passed (and the practical!). Again, that was a fortunate experience, not very often seen in labs. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA From barryrittman <@t> gmail.com Mon Aug 11 15:45:05 2014 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Mon Aug 11 15:45:13 2014 Subject: [Histonet] RE:On the lighter side... In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC881B4382E6@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC881B4382E6@D1PWPEXMBX05.mdanderson.edu> Message-ID: Well fixed in the histological sense I hope. Barry On Mon, Aug 11, 2014 at 2:52 PM, Mayer,Toysha N wrote: > Tim, > > Just saw your post about the 'marketable skill'. Funny those were my > mother's exact words while I was in college. She didn't care what I > majored in, as long as I got a marketable skill along the way. > The best advice I've ever gotten. > Thanks Maria!!! (my mother) > > Sincerely, > > Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) > Instructor/Education Coordinator > Program in Histotechnology > School of Health Professions > UT M.D. Anderson Cancer Center > 713.563-3481 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: 08 August 2014 19:26 > To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, > 1992. Electron Microscopy Technologist, #604, 1982. > > Like most, I never heard of "histology" until I walked into a hospital lab > on my first day as an EM tech. I had seen slides made in college, but no > one ever mentioned it could be an actual profession. I was more taken with > the electron microscope, and there was (is) a 2-year program at the > community college in the town I grew up in (Delta College, Stockton, CA). > So AFTER getting a BA in Zoology, I went there to get a marketable skill. > At that time EM was still used for tumor dx, so when I started it was about > half tumor, half kidney. I was lucky enough to get involved in histology > and set up the IHC lab at the small community hospital I worked at (as an > EM tech) and so ended up phasing myself almost out of an EM job. The IHC > took over all the tumor dx from EM. Later I left EM altogether and did IHC > exclusively for 15 years. But, like most, I learned Histotechnology on the > job but was lucky enough to work for a pathologist who believed in > developing his techs - to the point of paying for meetings out of his own > pocket. Only now do I know how fortunate I was to work for someone like > that. Because of him we had developed a culture in the small histo lab (4 > men!!) of learning. We studied together one night a week for the HT exam > and all passed (and the practical!). Again, that was a fortunate > experience, not very often seen in labs. > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies UC San Francisco Medical Center San Francisco, CA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gayle.callis <@t> bresnan.net Mon Aug 11 16:35:09 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Mon Aug 11 16:35:19 2014 Subject: [Histonet] Re: Weight Loss/Weight Gain decalcification endpoint test Message-ID: <000601cfb5ac$22447d70$66cd7850$@bresnan.net> Trevor and Jennifer, This method originally came from Mawhinney et al. Control of rapid nitric acid decalcification J Clin Pathol 1984 37:1409-1415 and was cited by Cathy Sanderson (Mayton)in a publication using EDTA, found in Biotechnic and Histochemistry. Mawhinney acutally did a chemical test on final acid change to see that calcium was not present, but we never had to do that. If you end up with a bit of residual calcium in block, I would surface decalcify at microtomy. I used it for years when we downsized and gave away our FAXITRON. Radiography is still the most accurate, and if you had either a micro CT or digital FAXITRON available, it would be a better test. I used Cathy's method and will be happy to send the original publication for anyone's files/future referencing. A chemical test for EDTA is a pain to do if a FAXITRON is not available. I have put the full method below, with a bit more detail. WEIGHT LOSS/WEIGHT GAIN ENDPOINT TEST This is a method of choice for EDTA decalcification although it was originally used by Mawhinney et al for testing nitric acid decalcification. Many samples can be decalcified together in one container, i.e. 25 mouse femurs in 1 liter of 10% formic acid. If all the samples are the same size, i.e. mouse femurs, tibia, paws, choose several as representative samples and test only those to save time. Always suspend bones in the decalcifier. Requires a balance that reads in milligrams to 3 places for greatest accuracy. Specimen must be blotted free of fluid for accurate weighing each time you weigh the sample. We suspend bones in nylon specimen bags for easy removal to weigh. Bags can be marked with pencil too. Technique: 1. Rinse NBF off bone, blot with paper towel, WEIGH BONE, RECORD BEGINNING WEIGHT. Suspend bone in acid or EDTA decalcifier. During acid decalcification CO2 bubbles are given off, so stir during decalcification to release bubbles or small samples will float. EDTA does not create CO2 bubbles, only acids. Large bones can be started at end of day in acid decalcifier and sit overnight with testing the next morning. 2. After 4 to 5 hours in acid or overnight in EDTA, remove bone, rinse with water, BLOT, weigh. RECORD WEIGHT. If bone shows loss of weight, change acid decalcifier to refresh acid. Return samples to resume decalcification, and repeat as many times as necessary. EDTA should be changed but not as often as acid. Always use a large volume of decalcifier i.e. 20:1 or more. Remove bone from specimen bag, and place in weighing boat to protect balance from acids/EDTA. 3. When bone begins to GAIN WEIGHT, the bone is decalcified. Once calcium is removed, water is taken on and the weight increases. This water does not affect the bone. 4. Rinse bone with running tap water for an hour or longer to remove these decalcifiers. Either store in 70% alcohol or process. Store endpoint tested decalcified bones in 70% alcohol while waiting for other samples to finish decalcifying and mass processing run. Reminders: For EDTA, one can suspend bones and check every day for accuracy but bones can be left in the EDTA over a weekend or several days without damage as long as the bones were well fixed. Acid decalcified bones cannot be left over a weekend, remove from acid, put in NBF to stop decalcification. Bones should be endpoint tested before stopping decalcification so you can resume decalcification on the next working day. Rinse off NBF briefly before resuming decalcification. Do not overexpose bones to acids or you will damage antigens and nuclear staining. Enjoy the method, as it truly is fast and easy. Gayle M. Callis HTL/HT/MT(ASCP) ____________________________________________________________________________ _____ Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald < JMacDonald <@t> mtsac.edu> Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: histonet <@t> lists.utsouthwestern.edu; histonet-bounces <@t> lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" < WaitT <@t> livemail.uthscsa.edu> To: " histonet <@t> lists.utsouthwestern.edu" < histonet <@t> lists.utsouthwestern.edu> Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces <@t> lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Mon Aug 11 18:04:32 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Mon Aug 11 18:04:43 2014 Subject: [Histonet] Consult for taking abs from RUO to IVD Message-ID: Colleagues, I am consulting with an antibody vendor who needs a consultant to help them take their abs from RUO to IVD. If you know anyone who does this type of work you could recommend would you please have them contact me directly and I will pass their contact info on. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From Susan.Walzer <@t> HCAHealthcare.com Tue Aug 12 01:58:14 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Tue Aug 12 01:58:21 2014 Subject: [Histonet] On the lighter side... In-Reply-To: References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FBFFC425D@FWDCWPMSGCMS09.hca.corpad.net> Also heard" Histotechs are good embed!!!" LOL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Tue Aug 12 02:49:24 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Tue Aug 12 02:47:28 2014 Subject: [Histonet] RE: Cytospin validation Message-ID: <013e01cfb601$f0da61e0$d28f25a0$@rowaihi@alborglaboratories.com> Good day colleagues Ignoring my previous message what it means: no one did validation for Cytospin !! or no one want to share his information ?? or no one received my message !!?? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From: Jamal [mailto:j.rowaihi@alborglaboratories.com] Sent: Monday, August 11, 2014 1:47 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Cytospin validation Hi I need to do validation for my Shandon Cytospin 4, can anyone send me the validation procedure and form. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From mjones <@t> metropath.com Tue Aug 12 08:00:08 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Aug 12 08:00:16 2014 Subject: [Histonet] On the lighter side... In-Reply-To: <4BF03F5404EBDE409AF9232DA74B9DED2FBFFC425D@FWDCWPMSGCMS09.hca.corpad.net> References: <005c01cfb26e$cac9e750$605db5f0$@caplab.org> <761E2B5697F795489C8710BCC72141FF367918C2@ex07.net.ucsf.edu> <4BF03F5404EBDE409AF9232DA74B9DED2FBFFC425D@FWDCWPMSGCMS09.hca.corpad.net> Message-ID: Ha ha!! Good one - Michael Ann On 8/12/14, 12:58 AM, "Susan.Walzer@HCAHealthcare.com" wrote: >Also heard" Histotechs are good embed!!!" LOL > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles >Claire >Sent: Monday, August 11, 2014 12:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > > >Old histologists never die, they are just well fixed... >Claire >________________________________________ >From: histonet-bounces@lists.utsouthwestern.edu >[histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann >Jones [mjones@metropath.com] >Sent: Monday, August 11, 2014 9:07 AM >To: Edwards, Richard; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >25 years, ("what?s that in micron?s??) Bernice, you are too funny!! >(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) >Histology Manager Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: > >>Sniffed my first formalin and saw first post-mortem November 1965. >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Tue Aug 12 11:18:12 2014 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Aug 12 11:18:27 2014 Subject: [Histonet] looking for TS replacement in Histology due to retirement Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B813@HPEMX3.HealthPartners.int> Histology Technical Specialist Position in St. Paul, MN Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and "Best Care, Best Experience", has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will oversee the technical component of a work team in the histology laboratory including; implementation of methodologies, supply ordering and inventory management, interpretation of laboratory results for physicians and other hospital staff; performance of laboratory tests in the section assigned; assisting in performance evaluations; assisting in supervision of testing personnel; development and implementation of training and competency programs for histotechnicians and histology students interns, and to perform related duties as assigned. Qualifications: Graduation from an accredited college or university with an Associates (AA) or bachelor's degree in medical technology, histology, biology or related field; plus specialized training in laboratory science. A master's degree in related field may substitute for the above educational and registration requirements. Bachelor's degree preferred. At least four (4) years of experience as a Histotechnician or Histotechnologist or related field with at least two (2) years of experience in the specialty occurring within the last four years. How to Apply Apply online at www.regionshospital.com Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From Debra.Ortiz <@t> advocatehealth.com Tue Aug 12 12:56:30 2014 From: Debra.Ortiz <@t> advocatehealth.com (Ortiz, Debra) Date: Tue Aug 12 12:56:37 2014 Subject: [Histonet] protocols Message-ID: <89a14d0a11da44a58a9072d7741e1812@BLUPR07MB083.namprd07.prod.outlook.com> Good afternoon everyone, We are currently looking at or protocols for microtomy. Currently we cut 22 unstained slides for all lung bxs, 10 unstained for all other FNAs, and 6 unstained for liver bxs. Can anyone share their current microtomy protocols? The pathologists are willing to take a look at some change, just would like to know what other institutions are doing? Thanks Debi This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. From Wanda.Smith <@t> HCAhealthcare.com Tue Aug 12 13:06:12 2014 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Tue Aug 12 13:06:43 2014 Subject: [Histonet] RE: protocols In-Reply-To: <89a14d0a11da44a58a9072d7741e1812@BLUPR07MB083.namprd07.prod.outlook.com> References: <89a14d0a11da44a58a9072d7741e1812@BLUPR07MB083.namprd07.prod.outlook.com> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27FD300F1A@NADCWPMSGCMS03.hca.corpad.net> Good Afternoon, We just pick-up 7-8 sections on treated slides on tiny CT Bxs, liver Bxs and lung bxs. We keep them for a week or two and if they are not used, we throw them away. Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ortiz, Debra Sent: Tuesday, August 12, 2014 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] protocols Good afternoon everyone, We are currently looking at or protocols for microtomy. Currently we cut 22 unstained slides for all lung bxs, 10 unstained for all other FNAs, and 6 unstained for liver bxs. Can anyone share their current microtomy protocols? The pathologists are willing to take a look at some change, just would like to know what other institutions are doing? Thanks Debi This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Aug 12 13:20:20 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Aug 12 13:20:32 2014 Subject: [Histonet] RE: protocols In-Reply-To: <89a14d0a11da44a58a9072d7741e1812@BLUPR07MB083.namprd07.prod.outlook.com> References: <89a14d0a11da44a58a9072d7741e1812@BLUPR07MB083.namprd07.prod.outlook.com> Message-ID: <761E2B5697F795489C8710BCC72141FF3679221E@ex07.net.ucsf.edu> Wow, that's a lot of unstained! We only cut unstained if requested and we don't cut anything like that for standing protocols. The most we have is 3-5 unstained slides for a the breast protocols and 2 on some liver protocols. The most extensive is Hirschprung's protocol that goes up to 26 slides on a second round, alternating H&E and unstained. A few years ago we brought up to our medical director that we had thousands of unstained slides sitting in files so investigated and revamped things. It had been a long-standing practice to always cut 2 extra unstained on ANY immuno order "just in case." But that was largely due to very long TAT on IHC staining. Now we have it down to 6-18 hours TAT so if there is a need for more stains they just order more and we cut them as needed. And now we have barcoded slides, including unstained, so they can see in the system exactly how many unstained are left on a particular block. And they can order a stain on the exact unstained slide they want. That alleviates concern that we don't have unstained, or they can't be found. We keep all but FISH and PCR unstained slides in the histo lab. Might this excessive unstained ordering be due to concern about cutting away tissue on initial cutting and recuts? Or is it TAT? Get to the root cause of this and you will find some things to work on in the lab that could alleviate it. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ortiz, Debra Sent: Tuesday, August 12, 2014 10:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] protocols Good afternoon everyone, We are currently looking at or protocols for microtomy. Currently we cut 22 unstained slides for all lung bxs, 10 unstained for all other FNAs, and 6 unstained for liver bxs. Can anyone share their current microtomy protocols? The pathologists are willing to take a look at some change, just would like to know what other institutions are doing? Thanks Debi This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Aug 12 13:54:17 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Tue Aug 12 13:54:28 2014 Subject: [Histonet] Liquid cover glass for H&E stained cell culture in 6 well plates; toluidine blue stain for cell cultures Message-ID: <000201cfb65e$d35be5f0$7a13b1d0$@bresnan.net> What are people using as a liquid cover glass for stained cells in 6 well culture plates to be view with inverted microscope with having that annoying refractile, funky look to the cells. Cells are grown in 6 well culture plates and with previous cultures, been stained with Hema 3 (hematological stain) and Giemsa of which neither has been successful to see cell membranes and nuclei clearly. Viewing is done with inverted microscope and cells are counted. I know there are some stains that can be viewed left under water with inverted scope. H&E obviously cannot be left under water due to eosin washing out of cytoplasm. A possibility is letting H&E or T blue stained cells dry, then put a thin layer of permanent mounting media to flow over the cells to fill in spaces, let dry and view with inverted microscope. I think one could do an old bonehead trick we used on surface stained, thicker ground MMA embedded undecalcified bone, flood with immersion oil and put a cover glass on top. Very messy!!! However one could let H&E dry after last alcohol rinse, put a layer of immersion oil over the cells, and view with inverted microscope. Anything to fill in the cellular spaces on a confluent sheet of cells but let the oil be the cover glass. Growing cells on a cover glass or on slide with wells/gaskets is not an option. Any suggested stains with protocols would be appreciated too with thoughts here about using Toluidine blue. The recipe for making up the stain, staining method and preferred fixative would be appreciated. You can send information privately if you wish. A challenge to be sure Thanks Gayle Callis HTL/HT/MT(ASCP) From suetp918 <@t> comcast.net Tue Aug 12 14:43:17 2014 From: suetp918 <@t> comcast.net (Sue) Date: Tue Aug 12 14:43:33 2014 Subject: [Histonet] RE: protocols In-Reply-To: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27FD300F1A@NADCWPMSGCMS03.hca.corpad.net> Message-ID: <1312820917.917529.1407872597262.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> hello We collect one unstained for postate biopsies, 5 unstained for lungs (we id each slide with a level number and do 5 unstained), if the biopsy is small the histotech?will?take come unstained slides and hold them for about a week, we actually place a dot on the block so the techs know there are unstained.? If the pathologistrs wants unstained requested upfront they are ordered by the PA's. We found that we were discaring ?almost a case of unstaained slides a month , since they were plus slides that is about $400 per month.? We do not seem to have many issues, although sometimes you just have that case that will haunt you. S Paturzo TJUH From Carol.Epp <@t> saskatoonhealthregion.ca Tue Aug 12 15:17:57 2014 From: Carol.Epp <@t> saskatoonhealthregion.ca (Epp, Carol SktnHR) Date: Tue Aug 12 15:18:04 2014 Subject: [Histonet] Charged slides for Giemsa In-Reply-To: <4cfeb676-9008-4194-b31f-770518e8c571@EX-EDG-V1.sktnhr.ca> References: <4cfeb676-9008-4194-b31f-770518e8c571@EX-EDG-V1.sktnhr.ca> Message-ID: <4408321938F9704A999D4837C92E4D94C4D73023@EX-MBD-P1.sktnhr.ca> Has anybody ever had a problem using charged slides for Giemsa? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, August 11, 2014 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. looking for used unwanted Tissue-tek paraffin block files (LeRoy Brown) 2. RE: On the lighter side... (Sebree Linda A) 3. RE: On the lighter side... (Susan.Walzer@HCAHealthcare.com) 4. Cytospin validation (Jamal) 5. RE: On the lighter side... (Edwards, Richard) 6. Laboratory Refrigerator w glass doors (Susan Foreman) 7. Re: On the lighter side... (Michael Ann Jones) 8. Anti MMP-9 (Johns, Laura [ETHUS]) 9. RE: On the lighter side... (Ingles Claire) 10. Re: On the lighter side... (Michael Ann Jones) 11. Ventana Symphony (Fawn Bomar) 12. Need microvascular (MVD) staining and quantification of tumro vasculature. (Hans B Snyder) ---------------------------------------------------------------------- Message: 1 Date: Sun, 10 Aug 2014 13:27:58 -0700 From: "LeRoy Brown" <11z@comcast.net> Subject: [Histonet] looking for used unwanted Tissue-tek paraffin block files To: Message-ID: <00ae01cfb4d9$934b9220$b9e2b660$@comcast.net> Content-Type: text/plain; charset="us-ascii" Hi. I need to find some used tissue-tek block file cabinets. Can you contact me if you have some I could purchase or take off your hands?? Contact Roy at 11z@comcast.net FYI: I would be using these in my lab and not planning on selling them. If you know of someone who may be selling used files let me know too, I cannot afford new ones. They just cost way too much. Roy Brown HT(ASCP) HTL ------------------------------ Message: 2 Date: Sun, 10 Aug 2014 21:50:58 +0000 From: Sebree Linda A Subject: RE: [Histonet] On the lighter side... To: Jamal , 'Vincent Rivera' , 'Douglas Porter' , "histonet@lists.utsouthwestern.edu" Message-ID: <77DD817201982748BC67D7960F2F76AF0C7C6D@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" 38 years and looking forward to retirement in a few years. Linda A. Sebree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Jamal [j.rowaihi@alborglaboratories.com] Sent: Saturday, August 09, 2014 4:47 AM To: 'Vincent Rivera'; 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Similar as me So strong, proud with high confidence Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vincent Rivera Sent: Thursday, August 07, 2014 11:58 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 15 Years and still going strong :) Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 11 Aug 2014 01:58:03 -0500 From: Subject: RE: [Histonet] On the lighter side... To: , Cc: histonet@lists.utsouthwestern.edu, Richard.Cartun@hhchealth.org Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FBFED1D42@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="utf-8" For me 36years..will not be 37, I retire in 3 weeks!!!!! Off to visit my son and family in England! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Friday, August 08, 2014 11:01 AM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard Subject: Re: [Histonet] On the lighter side... 3 years in histology, 2 years registered! Beth Brinegar HTL (ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala wrote: > Too funny Richard - you would pass with flying colors > > For me its 31 years - HTL-930 > > I have really been blessed, I love my job and I have really enjoyed my > career. Every day there is something new to learn or to work on, for > example the lab is putting the final touches on a poster that will be > displayed at NSH this year, working on that has been exciting and a > lot of fun too. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [ > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, > Richard [ Richard.Cartun@hhchealth.org] > Sent: Friday, August 08, 2014 8:32 AM > To: Douglas Porter; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > "36" years; however, not registered. Hopefully, I can take the QIHC > someday and pass. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic > Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter > Sent: Thursday, August 07, 2014 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] On the lighter side... > > How long have you been a registered histotech? 36 years here. You??? > > > > Douglas A. Porter, HT (ASCP) > Grossing Technician > IT Coordinator > > Cancer Registrar > > > CAP-Lab, PLC > 2508 South Cedar Street > Lansing, MI 48910-3138 > > 517-372-5520 (phone) > 517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution > is prohibited. If you are not the intended recipient, or an employee > or agent responsible for delivering the message to the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 11 Aug 2014 13:46:33 +0300 From: "Jamal" Subject: [Histonet] Cytospin validation To: Message-ID: <011101cfb551$85e59db0$91b0d910$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="us-ascii" Hi I need to do validation for my Shandon Cytospin 4, can anyone send me the validation procedure and form. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com ------------------------------ Message: 5 Date: Mon, 11 Aug 2014 11:17:49 +0000 From: "Edwards, Richard" Subject: RE: [Histonet] On the lighter side... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Sniffed my first formalin and saw first post-mortem November 1965. Richard Edwards Leicester U.K. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: 08 August 2014 19:26 To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 1992. Electron Microscopy Technologist, #604, 1982. Like most, I never heard of "histology" until I walked into a hospital lab on my first day as an EM tech. I had seen slides made in college, but no one ever mentioned it could be an actual profession. I was more taken with the electron microscope, and there was (is) a 2-year program at the community college in the town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a marketable skill. At that time EM was still used for tumor dx, so when I started it was about half tumor, half kidney. I was lucky enough to get involved in histology and set up the IHC lab at the small community hospital I worked at (as an EM tech) and so ended up phasing myself almost out of an EM job. The IHC took over all the tumor dx from EM. Later I left EM altogether and did IHC exclusively for 15 years. But, like most, I learned Histotechnology on the job but was lucky enough to work for a pathologist who believed in developing his techs - to the point of paying for meetings out of his own pocket. Only now do I know how fortunate I was to work for someone like that. Because of him we had developed a culture in the small histo lab (4 men!!) of learning. We studied together one night a week for the HT exam and all passed (and the practical!). Again, that was a fortunate experience, not very often seen in labs. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 11 Aug 2014 08:37:21 -0400 From: "Susan Foreman" Subject: [Histonet] Laboratory Refrigerator w glass doors To: Message-ID: <003601cfb561$0005f7c0$0011e740$@com> Content-Type: text/plain; charset="us-ascii" We are looking at a new laboratory refrigerator >23ft for our IHC department. What models would you recommend and which to avoid? It's a Monday! Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 ------------------------------ Message: 7 Date: Mon, 11 Aug 2014 14:07:28 +0000 From: Michael Ann Jones Subject: Re: [Histonet] On the lighter side... To: "Edwards, Richard" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > > > > Richard Edwards > > > Leicester U.K. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, >Timothy >Sent: 08 August 2014 19:26 >To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > >Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL >1369, 1992. Electron Microscopy Technologist, #604, 1982. > >Like most, I never heard of "histology" until I walked into a hospital >lab on my first day as an EM tech. I had seen slides made in college, >but no one ever mentioned it could be an actual profession. I was more >taken with the electron microscope, and there was (is) a 2-year program >at the community college in the town I grew up in (Delta College, >Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a >marketable skill. At that time EM was still used for tumor dx, so when >I started it was about half tumor, half kidney. I was lucky enough to >get involved in histology and set up the IHC lab at the small community >hospital I worked at (as an EM tech) and so ended up phasing myself >almost out of an EM job. The IHC took over all the tumor dx from EM. >Later I left EM altogether and did IHC exclusively for 15 years. But, >like most, I learned Histotechnology on the job but was lucky enough to >work for a pathologist who believed in developing his techs - to the >point of paying for meetings out of his own pocket. Only now do I know >how fortunate I was to work for someone like that. Because of him we >had developed a culture in the small histo lab (4 men!!) of learning. >We studied together one night a week for the HT exam and all passed >(and the practical!). Again, that was a fortunate experience, not very >often seen in labs. > >Tim Morken >Supervisor, Histology, Electron Microscopy and Neuromuscular Special >Studies UC San Francisco Medical Center San Francisco, CA > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas >Porter >Sent: Thursday, August 07, 2014 11:39 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] On the lighter side... > >How long have you been a registered histotech? 36 years here. You??? > > > >Douglas A. Porter, HT (ASCP) >Grossing Technician >IT Coordinator > >Cancer Registrar > > >CAP-Lab, PLC >2508 South Cedar Street >Lansing, MI 48910-3138 > >517-372-5520 (phone) >517-372-5540 (fax) > > doug.porter@caplab.org > > www.caplab.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 11 Aug 2014 15:44:53 +0000 From: "Johns, Laura [ETHUS]" Subject: [Histonet] Anti MMP-9 To: "histonet@lists.utsouthwestern.edu" Message-ID: <0295CDAF7914EF4D810D7C5541F7CAE224A1228F@ITSUSRAGMDGC06.jnj.com> Content-Type: text/plain; charset="us-ascii" Hello, Can anyone recommend an antibody for MMP-9 that works in FFPE porcine tissue? Thanks, Laura ------------------------------ Message: 9 Date: Mon, 11 Aug 2014 15:59:53 +0000 From: Ingles Claire Subject: RE: [Histonet] On the lighter side... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > ------------------------------ Message: 10 Date: Mon, 11 Aug 2014 16:21:55 +0000 From: Michael Ann Jones Subject: Re: [Histonet] On the lighter side... To: Bernice Frederick , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="euc-kr" Oh, I ment to say ??Lots of tenure here and lots of brain cells here?? meaning this website and all histotechs here, not myself. Obviously I don??t have many brain cells left If I made it look like I was talking about myself. . .sheesh. . Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 10:18 AM, "Bernice Frederick" wrote: >It was Friday,I was getting goofy doing paperwork,....... > >Bernice Frederick HTL (ASCP) >Senior Research Tech >Pathology Core Facility >ECOGPCO-RL >Robert. H. Lurie Cancer Center >Northwestern University >710 N Fairbanks Court >Olson 8-421 >Chicago,IL 60611 >312-503-3723 >b-frederick@northwestern.edu > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael >Ann Jones >Sent: Monday, August 11, 2014 9:07 AM >To: Edwards, Richard; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >25 years, ("what??s that in micron??s???) Bernice, you are too funny!! >(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT >(ASCP) Histology Manager Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: > >>Sniffed my first formalin and saw first post-mortem November 1965. >> >> >> >> Richard Edwards >> >> >> Leicester U.K. >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>Morken, Timothy >>Sent: 08 August 2014 19:26 >>To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu >>Subject: RE: [Histonet] On the lighter side... >> >>Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL >>1369, 1992. Electron Microscopy Technologist, #604, 1982. >> >>Like most, I never heard of "histology" until I walked into a hospital >>lab on my first day as an EM tech. I had seen slides made in college, >>but no one ever mentioned it could be an actual profession. I was more >>taken with the electron microscope, and there was (is) a 2-year >>program at the community college in the town I grew up in (Delta >>College, Stockton, CA). So AFTER getting a BA in Zoology, I went there >>to get a marketable skill. At that time EM was still used for tumor >>dx, so when I started it was about half tumor, half kidney. I was >>lucky enough to get involved in histology and set up the IHC lab at >>the small community hospital I worked at (as an EM tech) and so ended >>up phasing myself almost out of an EM job. The IHC took over all the tumor dx from EM. >>Later I left EM altogether and did IHC exclusively for 15 years. But, >>like most, I learned Histotechnology on the job but was lucky enough >>to work for a pathologist who believed in developing his techs - to >>the point of paying for meetings out of his own pocket. Only now do I >>know how fortunate I was to work for someone like that. Because of him >>we had developed a culture in the small histo lab (4 men!!) of learning. >>We studied together one night a week for the HT exam and all passed >>(and the practical!). Again, that was a fortunate experience, not very >>often seen in labs. >> >>Tim Morken >>Supervisor, Histology, Electron Microscopy and Neuromuscular Special >>Studies UC San Francisco Medical Center San Francisco, CA >> >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>Douglas Porter >>Sent: Thursday, August 07, 2014 11:39 AM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] On the lighter side... >> >>How long have you been a registered histotech? 36 years here. You??? >> >> >> >>Douglas A. Porter, HT (ASCP) >>Grossing Technician >>IT Coordinator >> >>Cancer Registrar >> >> >>CAP-Lab, PLC >>2508 South Cedar Street >>Lansing, MI 48910-3138 >> >>517-372-5520 (phone) >>517-372-5540 (fax) >> >> doug.porter@caplab.org >> >> www.caplab.org >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 11 Aug 2014 16:47:43 +0000 From: Fawn Bomar Subject: [Histonet] Ventana Symphony To: "histonet@lists.utsouthwestern.edu" Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B9745C7A@EXCH-2K10.hrhs.com> Content-Type: text/plain; charset="iso-8859-1" Can anyone using the Ventana Symphony share its pros and cons with me? Would you recommend this stainer? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 12 Date: Mon, 11 Aug 2014 12:51:05 -0400 From: Hans B Snyder Subject: [Histonet] Need microvascular (MVD) staining and quantification of tumro vasculature. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Hello All, I have a customer who needs microvascular (MVD) staining and quantification of tumro vasculature, where staining is typically done using CD31. Is there anyone who can do this and willing to forward their information to me? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 17 ***************************************** From raghulj <@t> orchidpharma.com Tue Aug 12 23:50:07 2014 From: raghulj <@t> orchidpharma.com (raghulj@orchidpharma.com) Date: Tue Aug 12 23:51:53 2014 Subject: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18 In-Reply-To: <52ee1248-6af4-4f0b-b495-e764619dcfc5@DB3FFO11FD027.protection.gbl> References: <52ee1248-6af4-4f0b-b495-e764619dcfc5@DB3FFO11FD027.protection.gbl> Message-ID: Dear all, In the weight loss/gain method how would we fix the optimal endpoint to stop the decal process based on weight gain? Will weighing omit the other ways of judgement such as needle prick, physical examination. In our tox experiments we deal with lot of femurs in one stretch and weighing method looks interesting. Pl. comment. J.Raghul Senior Research Associate Orchid Chemicals and Pharamaceuticals Limited Toxicology, Chennai,India. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 12 August 2014 22:38 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: On the lighter side... (Shirley A. Powell) 2. Bcl-2 (Heather Knight) 3. IHC Steam Strips: (Jb) 4. RE: Are Paraffin Blocks Biohazard (Michael LaFriniere) 5. RE: On the lighter side... (Mark Turner) 6. RE: On the lighter side... (Bernice Frederick) 7. Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 8. Re: Weight Loss/Weight Gain Decal (Jennifer MacDonald) 9. RE: Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 10. RE: Histonet Digest, Vol 129, Issue 17 (Mayer,Toysha N) 11. RE:On the lighter side... (Mayer,Toysha N) 12. Re: RE:On the lighter side... (Barry Rittman) 13. Re: Weight Loss/Weight Gain decalcification endpoint test (gayle callis) 14. Consult for taking abs from RUO to IVD (Patsy Ruegg) 15. RE: On the lighter side... (Susan.Walzer@HCAHealthcare.com) 16. RE: Cytospin validation (Jamal) 17. Re: On the lighter side... (Michael Ann Jones) 18. looking for TS replacement in Histology due to retirement (Webb, Dorothy L) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Aug 2014 13:03:38 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] On the lighter side... To: Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE25BFB25FD10@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="iso-8859-1" I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 11 Aug 2014 13:18:57 -0400 From: Heather Knight Subject: [Histonet] Bcl-2 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi everyone- Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse tissue? If so, can you share both the protocol and the antibody information? We have tried numerous antibodies over the years with very limited success. Thank you for your help!! Best, Heather Knight ------------------------------ Message: 3 Date: Mon, 11 Aug 2014 10:18:53 -0700 From: Jb Subject: [Histonet] IHC Steam Strips: To: Histonet@lists.utsouthwestern.edu Message-ID: <375F4EF9-47B6-46CD-B5B1-B511309EF4AA@gmail.com> Content-Type: text/plain; charset=us-ascii Does anyone use IHC steam strips in their decloaking chamber? The question is do you run a new steam strip each run and log each individual run w/it's own steam strip? Thank you, Sent from my iPhone ------------------------------ Message: 4 Date: Mon, 11 Aug 2014 17:43:34 +0000 From: Michael LaFriniere Subject: RE: [Histonet] Are Paraffin Blocks Biohazard To: Dawn Bugge , "histonet@lists.utsouthwestern.edu" Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D296A0A37@AHCMSASEXCH00.my.ahc.local> Content-Type: text/plain; charset="us-ascii" Dawn, The only study I know of is on CJD crutsfeldt-Jacobs Disease (know to survive formalin fixation and routine processing protocols, the CDC web site has additional information, In my laboratories I put all blocks in hazardous waste for incineration disposal. It is not that costly just to be on the safe side. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Friday, August 08, 2014 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Paraffin Blocks Biohazard Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 11 Aug 2014 17:49:13 +0000 From: Mark Turner Subject: RE: [Histonet] On the lighter side... To: "Shirley A. Powell" , Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <643626B74DE2814D8537057F40E1A10B0CAD409D@CSI-MX-NODEA.CSI-LABS.local> Content-Type: text/plain; charset="iso-8859-1" Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 11 Aug 2014 17:53:31 +0000 From: Bernice Frederick Subject: RE: [Histonet] On the lighter side... To: Mark Turner , "Shirley A. Powell" , Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F3167@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="iso-8859-1" Or we'll live forever..... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Monday, August 11, 2014 12:49 PM To: Shirley A. Powell; Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 11 Aug 2014 19:26:57 +0000 From: "Wait, Trevor Jordan" Subject: [Histonet] Weight Loss/Weight Gain Decal To: "histonet@lists.utsouthwestern.edu" Message-ID: <1407785218488.59806@livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ------------------------------ Message: 8 Date: Mon, 11 Aug 2014 12:33:51 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Weight Loss/Weight Gain Decal To: "Wait, Trevor Jordan" Cc: "histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" To: "histonet@lists.utsouthwestern.edu" Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces@lists.utsouthwestern.edu Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 11 Aug 2014 19:35:29 +0000 From: "Wait, Trevor Jordan" Subject: RE: [Histonet] Weight Loss/Weight Gain Decal To: Jennifer MacDonald Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <1407785730771.24250@livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" To: "histonet@lists.utsouthwestern.edu" Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces@lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 11 Aug 2014 19:45:01 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 17 To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B4382A0@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Egads, I feel like a baby! 20 years registered, 3 yrs unregistered. By the way, I did see a former colleague with her ASCP certificate in her office, and the oldest sticker is from the year I was born. Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 2 Date: Sun, 10 Aug 2014 21:50:58 +0000 From: Sebree Linda A Subject: RE: [Histonet] On the lighter side... To: Jamal , 'Vincent Rivera' , 'Douglas Porter' , "histonet@lists.utsouthwestern.edu" Message-ID: <77DD817201982748BC67D7960F2F76AF0C7C6D@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" 38 years and looking forward to retirement in a few years. Linda A. Sebree ------------------------------ Message: 11 Date: Mon, 11 Aug 2014 19:52:19 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE:On the lighter side... To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B4382E6@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Tim, Just saw your post about the 'marketable skill'. Funny those were my mother's exact words while I was in college. She didn't care what I majored in, as long as I got a marketable skill along the way. The best advice I've ever gotten. Thanks Maria!!! (my mother) Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: 08 August 2014 19:26 To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 1992. Electron Microscopy Technologist, #604, 1982. Like most, I never heard of "histology" until I walked into a hospital lab on my first day as an EM tech. I had seen slides made in college, but no one ever mentioned it could be an actual profession. I was more taken with the electron microscope, and there was (is) a 2-year program at the community college in the town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a marketable skill. At that time EM was still used for tumor dx, so when I started it was about half tumor, half kidney. I was lucky enough to get involved in histology and set up the IHC lab at the small community hospital I worked at (as an EM tech) and so ended up phasing myself almost out of an EM job. The IHC took over all the tumor dx from EM. Later I left EM altogether and did IHC exclusively for 15 years. But, like most, I learned Histotechnology on the job but was lucky enough to work for a pathologist who believed in developing his techs - to the point of paying for meetings out of his own pocket. Only now do I know how fortunate I was to work for someone like that. Because of him we had developed a culture in the small histo lab (4 men!!) of learning. We studied together one night a week for the HT exam and all passed (and the practical!). Again, that was a fortunate experience, not very often seen in labs. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA ------------------------------ Message: 12 Date: Mon, 11 Aug 2014 15:45:05 -0500 From: Barry Rittman Subject: Re: [Histonet] RE:On the lighter side... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Well fixed in the histological sense I hope. Barry On Mon, Aug 11, 2014 at 2:52 PM, Mayer,Toysha N wrote: > Tim, > > Just saw your post about the 'marketable skill'. Funny those were my > mother's exact words while I was in college. She didn't care what I > majored in, as long as I got a marketable skill along the way. > The best advice I've ever gotten. > Thanks Maria!!! (my mother) > > Sincerely, > > Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) > Instructor/Education Coordinator > Program in Histotechnology > School of Health Professions > UT M.D. Anderson Cancer Center > 713.563-3481 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: 08 August 2014 19:26 > To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, > 1992. Electron Microscopy Technologist, #604, 1982. > > Like most, I never heard of "histology" until I walked into a hospital lab > on my first day as an EM tech. I had seen slides made in college, but no > one ever mentioned it could be an actual profession. I was more taken with > the electron microscope, and there was (is) a 2-year program at the > community college in the town I grew up in (Delta College, Stockton, CA). > So AFTER getting a BA in Zoology, I went there to get a marketable skill. > At that time EM was still used for tumor dx, so when I started it was about > half tumor, half kidney. I was lucky enough to get involved in histology > and set up the IHC lab at the small community hospital I worked at (as an > EM tech) and so ended up phasing myself almost out of an EM job. The IHC > took over all the tumor dx from EM. Later I left EM altogether and did IHC > exclusively for 15 years. But, like most, I learned Histotechnology on the > job but was lucky enough to work for a pathologist who believed in > developing his techs - to the point of paying for meetings out of his own > pocket. Only now do I know how fortunate I was to work for someone like > that. Because of him we had developed a culture in the small histo lab (4 > men!!) of learning. We studied together one night a week for the HT exam > and all passed (and the practical!). Again, that was a fortunate > experience, not very often seen in labs. > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies UC San Francisco Medical Center San Francisco, CA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Mon, 11 Aug 2014 15:35:09 -0600 From: "gayle callis" Subject: [Histonet] Re: Weight Loss/Weight Gain decalcification endpoint test To: Message-ID: <000601cfb5ac$22447d70$66cd7850$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Trevor and Jennifer, This method originally came from Mawhinney et al. Control of rapid nitric acid decalcification J Clin Pathol 1984 37:1409-1415 and was cited by Cathy Sanderson (Mayton)in a publication using EDTA, found in Biotechnic and Histochemistry. Mawhinney acutally did a chemical test on final acid change to see that calcium was not present, but we never had to do that. If you end up with a bit of residual calcium in block, I would surface decalcify at microtomy. I used it for years when we downsized and gave away our FAXITRON. Radiography is still the most accurate, and if you had either a micro CT or digital FAXITRON available, it would be a better test. I used Cathy's method and will be happy to send the original publication for anyone's files/future referencing. A chemical test for EDTA is a pain to do if a FAXITRON is not available. I have put the full method below, with a bit more detail. WEIGHT LOSS/WEIGHT GAIN ENDPOINT TEST This is a method of choice for EDTA decalcification although it was originally used by Mawhinney et al for testing nitric acid decalcification. Many samples can be decalcified together in one container, i.e. 25 mouse femurs in 1 liter of 10% formic acid. If all the samples are the same size, i.e. mouse femurs, tibia, paws, choose several as representative samples and test only those to save time. Always suspend bones in the decalcifier. Requires a balance that reads in milligrams to 3 places for greatest accuracy. Specimen must be blotted free of fluid for accurate weighing each time you weigh the sample. We suspend bones in nylon specimen bags for easy removal to weigh. Bags can be marked with pencil too. Technique: 1. Rinse NBF off bone, blot with paper towel, WEIGH BONE, RECORD BEGINNING WEIGHT. Suspend bone in acid or EDTA decalcifier. During acid decalcification CO2 bubbles are given off, so stir during decalcification to release bubbles or small samples will float. EDTA does not create CO2 bubbles, only acids. Large bones can be started at end of day in acid decalcifier and sit overnight with testing the next morning. 2. After 4 to 5 hours in acid or overnight in EDTA, remove bone, rinse with water, BLOT, weigh. RECORD WEIGHT. If bone shows loss of weight, change acid decalcifier to refresh acid. Return samples to resume decalcification, and repeat as many times as necessary. EDTA should be changed but not as often as acid. Always use a large volume of decalcifier i.e. 20:1 or more. Remove bone from specimen bag, and place in weighing boat to protect balance from acids/EDTA. 3. When bone begins to GAIN WEIGHT, the bone is decalcified. Once calcium is removed, water is taken on and the weight increases. This water does not affect the bone. 4. Rinse bone with running tap water for an hour or longer to remove these decalcifiers. Either store in 70% alcohol or process. Store endpoint tested decalcified bones in 70% alcohol while waiting for other samples to finish decalcifying and mass processing run. Reminders: For EDTA, one can suspend bones and check every day for accuracy but bones can be left in the EDTA over a weekend or several days without damage as long as the bones were well fixed. Acid decalcified bones cannot be left over a weekend, remove from acid, put in NBF to stop decalcification. Bones should be endpoint tested before stopping decalcification so you can resume decalcification on the next working day. Rinse off NBF briefly before resuming decalcification. Do not overexpose bones to acids or you will damage antigens and nuclear staining. Enjoy the method, as it truly is fast and easy. Gayle M. Callis HTL/HT/MT(ASCP) ____________________________________________________________________________ _____ Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald < JMacDonald <@t> mtsac.edu> Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: histonet <@t> lists.utsouthwestern.edu; histonet-bounces <@t> lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" < WaitT <@t> livemail.uthscsa.edu> To: " histonet <@t> lists.utsouthwestern.edu" < histonet <@t> lists.utsouthwestern.edu> Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces <@t> lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 11 Aug 2014 17:04:32 -0600 From: Patsy Ruegg Subject: [Histonet] Consult for taking abs from RUO to IVD To: "Histonet@Lists. Edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Colleagues, I am consulting with an antibody vendor who needs a consultant to help them take their abs from RUO to IVD. If you know anyone who does this type of work you could recommend would you please have them contact me directly and I will pass their contact info on. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net ------------------------------ Message: 15 Date: Tue, 12 Aug 2014 01:58:14 -0500 From: Subject: RE: [Histonet] On the lighter side... To: , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FBFFC425D@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" Also heard" Histotechs are good embed!!!" LOL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 12 Aug 2014 10:49:24 +0300 From: "Jamal" Subject: [Histonet] RE: Cytospin validation To: Message-ID: <013e01cfb601$f0da61e0$d28f25a0$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="us-ascii" Good day colleagues Ignoring my previous message what it means: no one did validation for Cytospin !! or no one want to share his information ?? or no one received my message !!?? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From: Jamal [mailto:j.rowaihi@alborglaboratories.com] Sent: Monday, August 11, 2014 1:47 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Cytospin validation Hi I need to do validation for my Shandon Cytospin 4, can anyone send me the validation procedure and form. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com ------------------------------ Message: 17 Date: Tue, 12 Aug 2014 13:00:08 +0000 From: Michael Ann Jones Subject: Re: [Histonet] On the lighter side... To: "Susan.Walzer@HCAHealthcare.com" , "CIngles@uwhealth.org" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="euc-kr" Ha ha!! Good one - Michael Ann On 8/12/14, 12:58 AM, "Susan.Walzer@HCAHealthcare.com" wrote: >Also heard" Histotechs are good embed!!!" LOL > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles >Claire >Sent: Monday, August 11, 2014 12:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > > >Old histologists never die, they are just well fixed... >Claire >________________________________________ >From: histonet-bounces@lists.utsouthwestern.edu >[histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann >Jones [mjones@metropath.com] >Sent: Monday, August 11, 2014 9:07 AM >To: Edwards, Richard; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >25 years, ("what??s that in micron??s???) Bernice, you are too funny!! >(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) >Histology Manager Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: > >>Sniffed my first formalin and saw first post-mortem November 1965. >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 12 Aug 2014 11:18:12 -0500 From: "Webb, Dorothy L" Subject: [Histonet] looking for TS replacement in Histology due to retirement To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B813@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Histology Technical Specialist Position in St. Paul, MN Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and "Best Care, Best Experience", has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will oversee the technical component of a work team in the histology laboratory including; implementation of methodologies, supply ordering and inventory management, interpretation of laboratory results for physicians and other hospital staff; performance of laboratory tests in the section assigned; assisting in performance evaluations; assisting in supervision of testing personnel; development and implementation of training and competency programs for histotechnicians and histology students interns, and to perform related duties as assigned. Qualifications: Graduation from an accredited college or university with an Associates (AA) or bachelor's degree in medical technology, histology, biology or related field; plus specialized training in laboratory science. A master's degree in related field may substitute for the above educational and registration requirements. Bachelor's degree preferred. At least four (4) years of experience as a Histotechnician or Histotechnologist or related field with at least two (2) years of experience in the specialty occurring within the last four years. How to Apply Apply online at www.regionshospital.com Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 18 ***************************************** THIS E-MAIL IS CONFIDENTIAL and intended solely for the use of the individual to whom it is addressed. If you are not the addressee, any disclosure, reproduction, copying, distribution, or other dissemination or use of this communication is strictly prohibited. Finally the recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email From tmcampbell <@t> fmh.org Wed Aug 13 06:36:06 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Wed Aug 13 06:36:13 2014 Subject: [Histonet] PT Message-ID: <3566D9E34287BE4B95372179009446A020D3339A@EXCHANGE.fmhnt.fmh.org> I need some help. I am a small GI lab in Maryland and need to find a proficiency testing program. I am only accredited by CLIA. I am only seeing things in the CLIA regulations and on other websites that just seems to focus on cytology PT. Does anyone know a program I can enroll in? Thanks. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From klaus.dern44 <@t> gmail.com Wed Aug 13 07:56:47 2014 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Wed Aug 13 07:56:50 2014 Subject: [Histonet] THICK AND THIN SECTIONING ? Message-ID: If you are using one of the following Microtomes and the advance mechanism is worn out. ( too much play) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA / JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome, because these Models are no longer supported by the Manufacturer. ( no parts availability ) Rather than replacing these excellent instruments, I have a PERMANENT solution to this problem. For Information contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com From rashmil_histotechnology <@t> yahoo.com Wed Aug 13 09:08:50 2014 From: rashmil_histotechnology <@t> yahoo.com (rashmil_histotechnology@yahoo.com) Date: Wed Aug 13 09:08:54 2014 Subject: [Histonet] Dubin Johnson syndrome Message-ID: <048B593F-AC7D-47FE-AE80-37067A6ABA20@yahoo.com> Hello, How specific is Fontana stain for Dubin Johnson syndrome? Thanks, Rashmil From gayle.callis <@t> bresnan.net Wed Aug 13 09:42:02 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Aug 13 09:42:17 2014 Subject: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18 In-Reply-To: References: <52ee1248-6af4-4f0b-b495-e764619dcfc5@DB3FFO11FD027.protection.gbl> Message-ID: <001001cfb704$c0a1b8d0$41e52a70$@bresnan.net> The best way to determine the final endpoint is radiography as it is the most sensitive if you have a FAXITRON. If you have this xray machine or a microCT, then you should use these for decalcification endpoint and not waste time with weight loss/weight gain method or any other method. When we do the weight loss/weight gain, we simply made the judgement call that the weight gain means no calcium is present and no further testing is required. It is considered bad practice to "prick" the bone as you may 1) create needle tract artifact into critical areas you might want to see 2) possibly dislodge a small pathological lesion from the bone 3) bending bone can dislodge tumor from bone . Consequently, we never did mechanical testing EVER! We would decalcify as many as 40 to 50 samples - a collection of NBF fixed mouse knees, or tibias, or paws at one time in a large 1 to 2 liters 10% to 15% formic acid. Having to weigh each and every one of these small bones was tedious and time consuming. We found that if we took representative samples of each kind of bone - 4 from experimental group, and 4 from control group and use these bones as the wt loss/wt gain samples, the remaining samples still decalcifying were so close in size (and weight), we had excellent decalcification without damaging the bone. One learns very quickly which murine leg bones decalcify faster than other with paws taking much more time due to the tiny bones so tightly packed together. The original Mawhinney et al weight gain/weight loss publication tested the last nitric acid change with a chemical test. Unfortunately, doing chemical testing with EDTA is even more tedious. Some people will actually let the bones sit a few hours longer in formic acid decalcifiers to ensure the calcium is removed but you may over expose and do acid hydrolysis the antigens, nuclei i.e. "overdecalcification" . You can do this but I suggest testing the bones again so see if that weight gain has increased or not. If not, then you may have left the bones in acid too long. It doesn't take long to damage staining with acid exposure. A chemical endpoint test is more accurate than weight loss/weight gain, but you can't be doing a huge number of samples in one container of decalcifying solution. This is probably the reason those authors did chemical test after weight loss/weight gain but with single samples. I know of one person who success doing chemical test for 4 to 5 bone slabs of same size and approximate weight, decalcfified in an acid solution, and did the chemical test from a single container of used, never stirred decalcifier for those 4 or 5 large samples. I will be happy to send the original publication to you via private email, and the chemical test method. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghulj@orchidpharma.com Sent: Tuesday, August 12, 2014 10:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18 Dear all, In the weight loss/gain method how would we fix the optimal endpoint to stop the decal process based on weight gain? Will weighing omit the other ways of judgement such as needle prick, physical examination. In our tox experiments we deal with lot of femurs in one stretch and weighing method looks interesting. Pl. comment. J.Raghul Senior Research Associate Orchid Chemicals and Pharamaceuticals Limited Toxicology, Chennai,India. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 12 August 2014 22:38 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: On the lighter side... (Shirley A. Powell) 2. Bcl-2 (Heather Knight) 3. IHC Steam Strips: (Jb) 4. RE: Are Paraffin Blocks Biohazard (Michael LaFriniere) 5. RE: On the lighter side... (Mark Turner) 6. RE: On the lighter side... (Bernice Frederick) 7. Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 8. Re: Weight Loss/Weight Gain Decal (Jennifer MacDonald) 9. RE: Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 10. RE: Histonet Digest, Vol 129, Issue 17 (Mayer,Toysha N) 11. RE:On the lighter side... (Mayer,Toysha N) 12. Re: RE:On the lighter side... (Barry Rittman) 13. Re: Weight Loss/Weight Gain decalcification endpoint test (gayle callis) 14. Consult for taking abs from RUO to IVD (Patsy Ruegg) 15. RE: On the lighter side... (Susan.Walzer@HCAHealthcare.com) 16. RE: Cytospin validation (Jamal) 17. Re: On the lighter side... (Michael Ann Jones) 18. looking for TS replacement in Histology due to retirement (Webb, Dorothy L) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Aug 2014 13:03:38 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] On the lighter side... To: Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE25BFB25FD10@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="iso-8859-1" I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 11 Aug 2014 13:18:57 -0400 From: Heather Knight Subject: [Histonet] Bcl-2 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi everyone- Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse tissue? If so, can you share both the protocol and the antibody information? We have tried numerous antibodies over the years with very limited success. Thank you for your help!! Best, Heather Knight ------------------------------ Message: 3 Date: Mon, 11 Aug 2014 10:18:53 -0700 From: Jb Subject: [Histonet] IHC Steam Strips: To: Histonet@lists.utsouthwestern.edu Message-ID: <375F4EF9-47B6-46CD-B5B1-B511309EF4AA@gmail.com> Content-Type: text/plain; charset=us-ascii Does anyone use IHC steam strips in their decloaking chamber? The question is do you run a new steam strip each run and log each individual run w/it's own steam strip? Thank you, Sent from my iPhone ------------------------------ Message: 4 Date: Mon, 11 Aug 2014 17:43:34 +0000 From: Michael LaFriniere Subject: RE: [Histonet] Are Paraffin Blocks Biohazard To: Dawn Bugge , "histonet@lists.utsouthwestern.edu" Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D296A0A37@AHCMSASEXCH00.my.ahc.local> Content-Type: text/plain; charset="us-ascii" Dawn, The only study I know of is on CJD crutsfeldt-Jacobs Disease (know to survive formalin fixation and routine processing protocols, the CDC web site has additional information, In my laboratories I put all blocks in hazardous waste for incineration disposal. It is not that costly just to be on the safe side. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Friday, August 08, 2014 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Paraffin Blocks Biohazard Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 11 Aug 2014 17:49:13 +0000 From: Mark Turner Subject: RE: [Histonet] On the lighter side... To: "Shirley A. Powell" , Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <643626B74DE2814D8537057F40E1A10B0CAD409D@CSI-MX-NODEA.CSI-LABS.local> Content-Type: text/plain; charset="iso-8859-1" Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 11 Aug 2014 17:53:31 +0000 From: Bernice Frederick Subject: RE: [Histonet] On the lighter side... To: Mark Turner , "Shirley A. Powell" , Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F3167@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="iso-8859-1" Or we'll live forever..... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Monday, August 11, 2014 12:49 PM To: Shirley A. Powell; Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 11 Aug 2014 19:26:57 +0000 From: "Wait, Trevor Jordan" Subject: [Histonet] Weight Loss/Weight Gain Decal To: "histonet@lists.utsouthwestern.edu" Message-ID: <1407785218488.59806@livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ------------------------------ Message: 8 Date: Mon, 11 Aug 2014 12:33:51 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Weight Loss/Weight Gain Decal To: "Wait, Trevor Jordan" Cc: "histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" To: "histonet@lists.utsouthwestern.edu" Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces@lists.utsouthwestern.edu Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 11 Aug 2014 19:35:29 +0000 From: "Wait, Trevor Jordan" Subject: RE: [Histonet] Weight Loss/Weight Gain Decal To: Jennifer MacDonald Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <1407785730771.24250@livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" To: "histonet@lists.utsouthwestern.edu" Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces@lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 11 Aug 2014 19:45:01 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 17 To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B4382A0@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Egads, I feel like a baby! 20 years registered, 3 yrs unregistered. By the way, I did see a former colleague with her ASCP certificate in her office, and the oldest sticker is from the year I was born. Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 2 Date: Sun, 10 Aug 2014 21:50:58 +0000 From: Sebree Linda A Subject: RE: [Histonet] On the lighter side... To: Jamal , 'Vincent Rivera' , 'Douglas Porter' , "histonet@lists.utsouthwestern.edu" Message-ID: <77DD817201982748BC67D7960F2F76AF0C7C6D@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" 38 years and looking forward to retirement in a few years. Linda A. Sebree ------------------------------ Message: 11 Date: Mon, 11 Aug 2014 19:52:19 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE:On the lighter side... To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B4382E6@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Tim, Just saw your post about the 'marketable skill'. Funny those were my mother's exact words while I was in college. She didn't care what I majored in, as long as I got a marketable skill along the way. The best advice I've ever gotten. Thanks Maria!!! (my mother) Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: 08 August 2014 19:26 To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 1992. Electron Microscopy Technologist, #604, 1982. Like most, I never heard of "histology" until I walked into a hospital lab on my first day as an EM tech. I had seen slides made in college, but no one ever mentioned it could be an actual profession. I was more taken with the electron microscope, and there was (is) a 2-year program at the community college in the town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a marketable skill. At that time EM was still used for tumor dx, so when I started it was about half tumor, half kidney. I was lucky enough to get involved in histology and set up the IHC lab at the small community hospital I worked at (as an EM tech) and so ended up phasing myself almost out of an EM job. The IHC took over all the tumor dx from EM. Later I left EM altogether and did IHC exclusively for 15 years. But, like most, I learned Histotechnology on the job but was lucky enough to work for a pathologist who believed in developing his techs - to the point of paying for meetings out of his own pocket. Only now do I know how fortunate I was to work for someone like that. Because of him we had developed a culture in the small histo lab (4 men!!) of learning. We studied together one night a week for the HT exam and all passed (and the practical!). Again, that was a fortunate experience, not very often seen in labs. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA ------------------------------ Message: 12 Date: Mon, 11 Aug 2014 15:45:05 -0500 From: Barry Rittman Subject: Re: [Histonet] RE:On the lighter side... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Well fixed in the histological sense I hope. Barry On Mon, Aug 11, 2014 at 2:52 PM, Mayer,Toysha N wrote: > Tim, > > Just saw your post about the 'marketable skill'. Funny those were my > mother's exact words while I was in college. She didn't care what I > majored in, as long as I got a marketable skill along the way. > The best advice I've ever gotten. > Thanks Maria!!! (my mother) > > Sincerely, > > Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education > Coordinator Program in Histotechnology School of Health Professions UT > M.D. Anderson Cancer Center > 713.563-3481 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: 08 August 2014 19:26 > To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, > 1992. Electron Microscopy Technologist, #604, 1982. > > Like most, I never heard of "histology" until I walked into a hospital lab > on my first day as an EM tech. I had seen slides made in college, but no > one ever mentioned it could be an actual profession. I was more taken with > the electron microscope, and there was (is) a 2-year program at the > community college in the town I grew up in (Delta College, Stockton, CA). > So AFTER getting a BA in Zoology, I went there to get a marketable skill. > At that time EM was still used for tumor dx, so when I started it was about > half tumor, half kidney. I was lucky enough to get involved in histology > and set up the IHC lab at the small community hospital I worked at (as an > EM tech) and so ended up phasing myself almost out of an EM job. The IHC > took over all the tumor dx from EM. Later I left EM altogether and did IHC > exclusively for 15 years. But, like most, I learned Histotechnology on the > job but was lucky enough to work for a pathologist who believed in > developing his techs - to the point of paying for meetings out of his own > pocket. Only now do I know how fortunate I was to work for someone like > that. Because of him we had developed a culture in the small histo lab (4 > men!!) of learning. We studied together one night a week for the HT exam > and all passed (and the practical!). Again, that was a fortunate > experience, not very often seen in labs. > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies UC San Francisco Medical Center San Francisco, CA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Mon, 11 Aug 2014 15:35:09 -0600 From: "gayle callis" Subject: [Histonet] Re: Weight Loss/Weight Gain decalcification endpoint test To: Message-ID: <000601cfb5ac$22447d70$66cd7850$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Trevor and Jennifer, This method originally came from Mawhinney et al. Control of rapid nitric acid decalcification J Clin Pathol 1984 37:1409-1415 and was cited by Cathy Sanderson (Mayton)in a publication using EDTA, found in Biotechnic and Histochemistry. Mawhinney acutally did a chemical test on final acid change to see that calcium was not present, but we never had to do that. If you end up with a bit of residual calcium in block, I would surface decalcify at microtomy. I used it for years when we downsized and gave away our FAXITRON. Radiography is still the most accurate, and if you had either a micro CT or digital FAXITRON available, it would be a better test. I used Cathy's method and will be happy to send the original publication for anyone's files/future referencing. A chemical test for EDTA is a pain to do if a FAXITRON is not available. I have put the full method below, with a bit more detail. WEIGHT LOSS/WEIGHT GAIN ENDPOINT TEST This is a method of choice for EDTA decalcification although it was originally used by Mawhinney et al for testing nitric acid decalcification. Many samples can be decalcified together in one container, i.e. 25 mouse femurs in 1 liter of 10% formic acid. If all the samples are the same size, i.e. mouse femurs, tibia, paws, choose several as representative samples and test only those to save time. Always suspend bones in the decalcifier. Requires a balance that reads in milligrams to 3 places for greatest accuracy. Specimen must be blotted free of fluid for accurate weighing each time you weigh the sample. We suspend bones in nylon specimen bags for easy removal to weigh. Bags can be marked with pencil too. Technique: 1. Rinse NBF off bone, blot with paper towel, WEIGH BONE, RECORD BEGINNING WEIGHT. Suspend bone in acid or EDTA decalcifier. During acid decalcification CO2 bubbles are given off, so stir during decalcification to release bubbles or small samples will float. EDTA does not create CO2 bubbles, only acids. Large bones can be started at end of day in acid decalcifier and sit overnight with testing the next morning. 2. After 4 to 5 hours in acid or overnight in EDTA, remove bone, rinse with water, BLOT, weigh. RECORD WEIGHT. If bone shows loss of weight, change acid decalcifier to refresh acid. Return samples to resume decalcification, and repeat as many times as necessary. EDTA should be changed but not as often as acid. Always use a large volume of decalcifier i.e. 20:1 or more. Remove bone from specimen bag, and place in weighing boat to protect balance from acids/EDTA. 3. When bone begins to GAIN WEIGHT, the bone is decalcified. Once calcium is removed, water is taken on and the weight increases. This water does not affect the bone. 4. Rinse bone with running tap water for an hour or longer to remove these decalcifiers. Either store in 70% alcohol or process. Store endpoint tested decalcified bones in 70% alcohol while waiting for other samples to finish decalcifying and mass processing run. Reminders: For EDTA, one can suspend bones and check every day for accuracy but bones can be left in the EDTA over a weekend or several days without damage as long as the bones were well fixed. Acid decalcified bones cannot be left over a weekend, remove from acid, put in NBF to stop decalcification. Bones should be endpoint tested before stopping decalcification so you can resume decalcification on the next working day. Rinse off NBF briefly before resuming decalcification. Do not overexpose bones to acids or you will damage antigens and nuclear staining. Enjoy the method, as it truly is fast and easy. Gayle M. Callis HTL/HT/MT(ASCP) ____________________________________________________________________________ _____ Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald < JMacDonald <@t> mtsac.edu> Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: histonet <@t> lists.utsouthwestern.edu; histonet-bounces <@t> lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" < WaitT <@t> livemail.uthscsa.edu> To: " histonet <@t> lists.utsouthwestern.edu" < histonet <@t> lists.utsouthwestern.edu> Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces <@t> lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 11 Aug 2014 17:04:32 -0600 From: Patsy Ruegg Subject: [Histonet] Consult for taking abs from RUO to IVD To: "Histonet@Lists. Edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Colleagues, I am consulting with an antibody vendor who needs a consultant to help them take their abs from RUO to IVD. If you know anyone who does this type of work you could recommend would you please have them contact me directly and I will pass their contact info on. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net ------------------------------ Message: 15 Date: Tue, 12 Aug 2014 01:58:14 -0500 From: Subject: RE: [Histonet] On the lighter side... To: , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FBFFC425D@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" Also heard" Histotechs are good embed!!!" LOL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 12 Aug 2014 10:49:24 +0300 From: "Jamal" Subject: [Histonet] RE: Cytospin validation To: Message-ID: <013e01cfb601$f0da61e0$d28f25a0$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="us-ascii" Good day colleagues Ignoring my previous message what it means: no one did validation for Cytospin !! or no one want to share his information ?? or no one received my message !!?? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From: Jamal [mailto:j.rowaihi@alborglaboratories.com] Sent: Monday, August 11, 2014 1:47 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Cytospin validation Hi I need to do validation for my Shandon Cytospin 4, can anyone send me the validation procedure and form. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com ------------------------------ Message: 17 Date: Tue, 12 Aug 2014 13:00:08 +0000 From: Michael Ann Jones Subject: Re: [Histonet] On the lighter side... To: "Susan.Walzer@HCAHealthcare.com" , "CIngles@uwhealth.org" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="euc-kr" Ha ha!! Good one - Michael Ann On 8/12/14, 12:58 AM, "Susan.Walzer@HCAHealthcare.com" wrote: >Also heard" Histotechs are good embed!!!" LOL > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles >Claire >Sent: Monday, August 11, 2014 12:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > > >Old histologists never die, they are just well fixed... >Claire >________________________________________ >From: histonet-bounces@lists.utsouthwestern.edu >[histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann >Jones [mjones@metropath.com] >Sent: Monday, August 11, 2014 9:07 AM >To: Edwards, Richard; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >25 years, ("what??s that in micron??s???) Bernice, you are too funny!! >(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) >Histology Manager Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: > >>Sniffed my first formalin and saw first post-mortem November 1965. >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 12 Aug 2014 11:18:12 -0500 From: "Webb, Dorothy L" Subject: [Histonet] looking for TS replacement in Histology due to retirement To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B813@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Histology Technical Specialist Position in St. Paul, MN Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and "Best Care, Best Experience", has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will oversee the technical component of a work team in the histology laboratory including; implementation of methodologies, supply ordering and inventory management, interpretation of laboratory results for physicians and other hospital staff; performance of laboratory tests in the section assigned; assisting in performance evaluations; assisting in supervision of testing personnel; development and implementation of training and competency programs for histotechnicians and histology students interns, and to perform related duties as assigned. Qualifications: Graduation from an accredited college or university with an Associates (AA) or bachelor's degree in medical technology, histology, biology or related field; plus specialized training in laboratory science. A master's degree in related field may substitute for the above educational and registration requirements. Bachelor's degree preferred. At least four (4) years of experience as a Histotechnician or Histotechnologist or related field with at least two (2) years of experience in the specialty occurring within the last four years. How to Apply Apply online at www.regionshospital.com Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 18 ***************************************** THIS E-MAIL IS CONFIDENTIAL and intended solely for the use of the individual to whom it is addressed. If you are not the addressee, any disclosure, reproduction, copying, distribution, or other dissemination or use of this communication is strictly prohibited. Finally the recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Wed Aug 13 09:53:12 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Wed Aug 13 09:53:18 2014 Subject: [Histonet] Dubin Johnson syndrome In-Reply-To: <048B593F-AC7D-47FE-AE80-37067A6ABA20@yahoo.com> References: <048B593F-AC7D-47FE-AE80-37067A6ABA20@yahoo.com> Message-ID: <1407941575889.83155@accuratediagnosticlabs.com> What tissue are you staining? Liver? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of rashmil_histotechnology@yahoo.com Sent: Wednesday, August 13, 2014 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dubin Johnson syndrome Hello, How specific is Fontana stain for Dubin Johnson syndrome? Thanks, Rashmil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Wed Aug 13 10:24:00 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Aug 13 10:24:42 2014 Subject: [Histonet] biopsy "bags" for processing - alternatives Message-ID: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org From mucram11 <@t> comcast.net Wed Aug 13 10:37:09 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Aug 13 10:37:27 2014 Subject: [Histonet] biopsy "bags" for processing - alternatives In-Reply-To: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> Message-ID: <2106639529.1328417.1407944229780.JavaMail.root@comcast.net> Our kidney and liver biopsies are placed in BX bags (tea bags).? The pathologist feel the nylon bags leave a pattern on the tissue and sponges are even worse.? The Gross Room staff and residents also dislike the nylon bags as they feel they are harder to handle?and stiff.? Then we in Histology feel exactly the same as Tim's description.? We have tried various things and keep going bag to "tea bag" style biopsy bags.? If anyone has come up with a better idea or product please let us all know.? Thank You, Pam Marcum UAMS ----- Original Message ----- From: "Timothy Morken" To: "Histonet" Sent: Wednesday, August 13, 2014 10:24:00 AM Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 ?(office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Wed Aug 13 10:41:04 2014 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Aug 13 10:41:12 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives In-Reply-To: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> Message-ID: For pesky animal hair (we are a veterinary lab) with very little tissue attached, we wrap in lens paper - one layer of paper on one side of tissue and all other layers of the paper envelope on other side of the tissue - and embed the entire package. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 13, 2014 9:24 AM To: Histonet Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Wed Aug 13 10:43:58 2014 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Wed Aug 13 10:44:24 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives In-Reply-To: <2106639529.1328417.1407944229780.JavaMail.root@comcast.net> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu>, <2106639529.1328417.1407944229780.JavaMail.root@comcast.net> Message-ID: <1407944638142.58953@metropath.com> Telfa pads? We've used those with success. Michael Ann Jones, HT(ASCP) Metropath, Denver, CO ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pam Marcum Sent: Wednesday, August 13, 2014 9:37 AM To: Timothy Morken Cc: Histonet Subject: Re: [Histonet] biopsy "bags" for processing - alternatives Our kidney and liver biopsies are placed in BX bags (tea bags). The pathologist feel the nylon bags leave a pattern on the tissue and sponges are even worse. The Gross Room staff and residents also dislike the nylon bags as they feel they are harder to handle and stiff. Then we in Histology feel exactly the same as Tim's description. We have tried various things and keep going bag to "tea bag" style biopsy bags. If anyone has come up with a better idea or product please let us all know. Thank You, Pam Marcum UAMS ----- Original Message ----- From: "Timothy Morken" To: "Histonet" Sent: Wednesday, August 13, 2014 10:24:00 AM Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.norton <@t> seattlechildrens.org Wed Aug 13 10:44:21 2014 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Wed Aug 13 10:44:43 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives In-Reply-To: <2106639529.1328417.1407944229780.JavaMail.root@comcast.net> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> <2106639529.1328417.1407944229780.JavaMail.root@comcast.net> Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD040E62DE5@PPWEXD01B.childrens.sea.kids> We wrap our specimens in End Wrap papers (cut in half). Takes more time I suppose than putting them in bags. Sally Norton, HT Seattle Childrens Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, August 13, 2014 08:37 To: Timothy Morken Cc: Histonet Subject: Re: [Histonet] biopsy "bags" for processing - alternatives Our kidney and liver biopsies are placed in BX bags (tea bags). The pathologist feel the nylon bags leave a pattern on the tissue and sponges are even worse. The Gross Room staff and residents also dislike the nylon bags as they feel they are harder to handle and stiff. Then we in Histology feel exactly the same as Tim's description. We have tried various things and keep going bag to "tea bag" style biopsy bags. If anyone has come up with a better idea or product please let us all know. Thank You, Pam Marcum UAMS ----- Original Message ----- From: "Timothy Morken" To: "Histonet" Sent: Wednesday, August 13, 2014 10:24:00 AM Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From POWELL_SA <@t> mercer.edu Wed Aug 13 10:50:24 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Aug 13 10:50:44 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives In-Reply-To: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BFB755138@MERCERMAIL.MercerU.local> Hi Tim and all, I recently did a search for cassettes that I can process tiny, and I mean tiny (we are talking gnat's eyes, not really), specimens and found some cassettes that were perfect for them and one for a little larger, but still tiny. The came from Cancer Diagnostics, no I do not get a kick back. They are called AutoSette Cassettes (Vortex Cornerless Biopsy Cassettes) and are concave with NO corners for the tiny pieces to get caught in. I deal with a lot of small and unique tissues/animals and these work perfectly. Repeating myself, but the VM1007 ones are for the really extra tiny specimens and the VM1005 are for the little larger specimens. You can get them in colors to differentiate types of sources. You can see them here. http://www.cancerdiagnostics.com/CDI_Products.aspx?pid=153 I must say I have not lost anything yet. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 13, 2014 11:24 AM To: Histonet Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Aug 13 10:52:51 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Aug 13 10:52:57 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives In-Reply-To: <1357F84B33D39A46BA015A8EC6ABCBD040E62DE5@PPWEXD01B.childrens.sea.kids> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> <2106639529.1328417.1407944229780.JavaMail.root@comcast.net> <1357F84B33D39A46BA015A8EC6ABCBD040E62DE5@PPWEXD01B.childrens.sea.kids> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012404E192@Mail2Node2.ad.uams.edu> One of our biggest issues is getting the residents and gross room staff to cut things appropriately. We need the biopsies laying out flat on the wet inter bag and they seem to have issues refolding after the placement is completed or fold part into the bag distorting it. We have tried to get them to pay more attention and decided to attempt to find another way if possible. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Norton, Sally Sent: Wednesday, August 13, 2014 10:44 AM To: 'Pam Marcum'; Timothy Morken Cc: Histonet Subject: [Histonet] RE: biopsy "bags" for processing - alternatives We wrap our specimens in End Wrap papers (cut in half). Takes more time I suppose than putting them in bags. Sally Norton, HT Seattle Childrens Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Wednesday, August 13, 2014 08:37 To: Timothy Morken Cc: Histonet Subject: Re: [Histonet] biopsy "bags" for processing - alternatives Our kidney and liver biopsies are placed in BX bags (tea bags). The pathologist feel the nylon bags leave a pattern on the tissue and sponges are even worse. The Gross Room staff and residents also dislike the nylon bags as they feel they are harder to handle and stiff. Then we in Histology feel exactly the same as Tim's description. We have tried various things and keep going bag to "tea bag" style biopsy bags. If anyone has come up with a better idea or product please let us all know. Thank You, Pam Marcum UAMS ----- Original Message ----- From: "Timothy Morken" To: "Histonet" Sent: Wednesday, August 13, 2014 10:24:00 AM Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From POWELL_SA <@t> mercer.edu Wed Aug 13 10:54:40 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Aug 13 10:55:30 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25BFB755138@MERCERMAIL.MercerU.local> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> <9BF995BC0E47744E9673A41486E24EE25BFB755138@MERCERMAIL.MercerU.local> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BFB75514E@MERCERMAIL.MercerU.local> Correction the number is VB1005 but you can get all the numbers from the website. http://www.cancerdiagnostics.com/CDI_Products.aspx?pid=153 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, August 13, 2014 11:50 AM To: Morken, Timothy; Histonet Subject: [Histonet] RE: biopsy "bags" for processing - alternatives Hi Tim and all, I recently did a search for cassettes that I can process tiny, and I mean tiny (we are talking gnat's eyes, not really), specimens and found some cassettes that were perfect for them and one for a little larger, but still tiny. The came from Cancer Diagnostics, no I do not get a kick back. They are called AutoSette Cassettes (Vortex Cornerless Biopsy Cassettes) and are concave with NO corners for the tiny pieces to get caught in. I deal with a lot of small and unique tissues/animals and these work perfectly. Repeating myself, but the VM1007 ones are for the really extra tiny specimens and the VM1005 are for the little larger specimens. You can get them in colors to differentiate types of sources. You can see them here. http://www.cancerdiagnostics.com/CDI_Products.aspx?pid=153 I must say I have not lost anything yet. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 13, 2014 11:24 AM To: Histonet Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Wed Aug 13 10:56:04 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Aug 13 10:57:01 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25BFB755138@MERCERMAIL.MercerU.local> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> <9BF995BC0E47744E9673A41486E24EE25BFB755138@MERCERMAIL.MercerU.local> Message-ID: <943347430.1332676.1407945364007.JavaMail.root@comcast.net> We tried the AutoSette and it did not work on longer biopsies as they curl.? Love the design and the gross room had issues with it in the cassette writer.? It would print two or three and jam.? We get from 35 to 100 biopsies a day so it was an issue. Pam ----- Original Message ----- From: "Powell, Shirley" To: "Timothy Morken" , "Histonet" Sent: Wednesday, August 13, 2014 10:50:24 AM Subject: [Histonet] RE: biopsy "bags" for processing - alternatives Hi Tim and all, I recently did a search for cassettes that I can process tiny, and I mean tiny (we are talking gnat's eyes, not really), specimens and found some cassettes that were perfect for them and one for a little larger, but still tiny. ?The came from Cancer Diagnostics, no I do not get a kick back. ?They are called AutoSette Cassettes (Vortex Cornerless Biopsy Cassettes) and are concave with NO corners for the tiny pieces to get caught in. ?I deal with a lot of small and unique tissues/animals and these work perfectly. ?Repeating myself, but the VM1007 ones are for the really extra tiny specimens and the VM1005 are for the little larger specimens. ?You can get them in colors to differentiate types of sources. ?You can see them here. ?http://www.cancerdiagnostics.com/CDI_Products.aspx?pid=153 I must say I have not lost anything yet. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 13, 2014 11:24 AM To: Histonet Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 ?(office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Aug 13 10:59:25 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Aug 13 10:59:39 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives In-Reply-To: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> Message-ID: Personally, I prefer the biopsy bags and find them easy to work with. I generally hold the bag in one hand (allows the paraffin to cool enough to hold the tissue) and use forceps to slowly open it. If it is an ECC or something similar, I can wrap the opened bag around a couple of fingers and use a small spatula to scrap. With a little practice, I have found it to be quick and efficient. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 13, 2014 11:24 AM To: Histonet Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From rjbuesa <@t> yahoo.com Wed Aug 13 11:54:43 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 13 12:01:46 2014 Subject: [Histonet] biopsy "bags" for processing - alternatives In-Reply-To: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> Message-ID: <1407948883.70229.YahooMailNeo@web120403.mail.ne1.yahoo.com> We never ever allowed the residents to wrap anything. They were instructed to leave everything in the sample bottle and later the histotech decided what to do. If there were many small pieces we filtered the sample through a tissue paper and processed it folded. Sometimes we used empty tea bags with very good results. Ren? J.? On Wednesday, August 13, 2014 11:25 AM, "Morken, Timothy" wrote: All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042? (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Wed Aug 13 12:17:40 2014 From: suetp918 <@t> comcast.net (Sue) Date: Wed Aug 13 12:17:59 2014 Subject: [Histonet] biopsy "bags" for processing - alternatives In-Reply-To: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> Message-ID: <1259267934.918388.1407950260092.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> We also use the nylon bags and have to pay extreme attention when embedding.? We no longer use sponges due to cross contamaination.? We use to buy tissue bags from Fisher and they were similar to the "blue" paper we use from Leica.? I have been looking at tea bag vendors (like we get Lipton and Salda tea in).? They are paper the only issue is the quantity you have to purchase.? Mopec make a bag but the issue the open end is even and this is a real pain when trying to get inside, as I mentioned before the old Fisher bags had one end longer than the other so you were able to place your forceps inside with ease.? Another reason I would like to replace the nylon bag is that when you get an aggregate of tissue some of it gets inside the mesh and if there is minimal tissue I fear you may miss some diagnostic material. Sue PaturzoTJUH From JJennings <@t> thedermlab.com Wed Aug 13 13:14:58 2014 From: JJennings <@t> thedermlab.com (Jonathan Jennings) Date: Wed Aug 13 13:15:25 2014 Subject: [Histonet] Lynx Tissue Processor Message-ID: <248395418C8C074A83C7D06081C4F016106BC2F0@DERMLAB-SBS.dermlab.local> Hello, I am looking at some cheap back up processors. Has anyone had any experience with the Australian Biomedical Lynx EL-290 microscopy Tissue Processor. Thanks in advance From nmhisto <@t> comcast.net Wed Aug 13 14:17:39 2014 From: nmhisto <@t> comcast.net (Sally) Date: Wed Aug 13 14:18:50 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives Message-ID: I always used lens paper. Wrap paper over tissue at an oblique angle so the forceps can grab a corner of the thin paper and peel away without losing tissue - whether hot or cold paraffin. Never lost a tissue... -----Original Message----- From: "Tom McNemar" Sent: ?8/?13/?2014 9:59 AM To: "'Morken, Timothy'" ; "Histonet" Subject: [Histonet] RE: biopsy "bags" for processing - alternatives Personally, I prefer the biopsy bags and find them easy to work with. I generally hold the bag in one hand (allows the paraffin to cool enough to hold the tissue) and use forceps to slowly open it. If it is an ECC or something similar, I can wrap the opened bag around a couple of fingers and use a small spatula to scrap. With a little practice, I have found it to be quick and efficient. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 13, 2014 11:24 AM To: Histonet Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Aug 13 14:22:29 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Aug 13 14:22:35 2014 Subject: [Histonet] biopsy "bags" for processing - alternatives In-Reply-To: <1407948883.70229.YahooMailNeo@web120403.mail.ne1.yahoo.com> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> <1407948883.70229.YahooMailNeo@web120403.mail.ne1.yahoo.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32012404E288@Mail2Node2.ad.uams.edu> We have no Histologist in the Gross Room and path assistants often do the biopsies as they meet CLIA guidelines for this. The residents are required to do certain biopsies and we have no control over it. The argument has been raging now for years and it is what it is so we live with it. You and others may disagree (as I do) however; we are not the ones making those choices. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 13, 2014 11:55 AM To: Morken, Timothy; Histonet Subject: Re: [Histonet] biopsy "bags" for processing - alternatives We never ever allowed the residents to wrap anything. They were instructed to leave everything in the sample bottle and later the histotech decided what to do. If there were many small pieces we filtered the sample through a tissue paper and processed it folded. Sometimes we used empty tea bags with very good results. Ren? J.? On Wednesday, August 13, 2014 11:25 AM, "Morken, Timothy" wrote: All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042? (office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mucram11 <@t> comcast.net Wed Aug 13 14:47:48 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Wed Aug 13 14:48:05 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives Message-ID: <771255633.1395072.1407959268100.JavaMail.root@comcast.net> If we grossed them we could control this.? However; we are physically a quarter mile ftom the Gross Room so we get what we get and have learned to live with it.? I have used lens paper in the past and it is not used here.? I also had more say in how the biopsies were handled and by whom than I have here so I just look for better ways that we might get approved.? Pam ----- Original Message ----- From: "Sally" To: "Tom McNemar" , "Timothy' 'Morken" , "Histonet" Sent: Wednesday, August 13, 2014 2:17:39 PM Subject: RE: [Histonet] RE: biopsy "bags" for processing - alternatives I always used lens paper. Wrap paper over tissue at an oblique angle so the forceps can grab ?a corner of the thin paper and peel away without losing tissue - whether hot or cold paraffin. Never lost a tissue... -----Original Message----- From: "Tom McNemar" Sent: ?8/?13/?2014 9:59 AM To: "'Morken, Timothy'" ; "Histonet" Subject: [Histonet] RE: biopsy "bags" for processing - alternatives Personally, I prefer the biopsy bags and find them easy to work with. ?I generally hold the bag in one hand (allows the paraffin to cool enough to hold the tissue) and use forceps to slowly open it. ?If it is an ECC or something similar, I can wrap the opened bag around a couple of fingers and use a small spatula to scrap. ?With a little practice, I have found it to be quick and efficient. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 13, 2014 11:24 AM To: Histonet Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 ?(office) tim.morken@ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Wed Aug 13 14:55:53 2014 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Aug 13 14:56:02 2014 Subject: [Histonet] On the lighter side... Message-ID: <5F06C3AD0B27264CA20CFA986C87882EC70CD7C5@EXCHANGEPV1.KGH.ON.CA> AKA Roll-call of the Histo-lifers! Started in Histo 1985. Spent 3.5 years in Hematology, but had to leave because I became bored with their incessant blood testing. Where were the interesting Histo bits and pieces I was so used to seeing and glomming? Our laboratory nearly-completely re-equipped with new tissue processors, Bond IHC stainers, Dako Special Stainer, Cerebro tracking system. Now on same level of playing field with much newer/younger Histo MLT's. My incessant babbling about the Technicons, AO microtomes, freon-cooling of specimens and sharpening of real, big ol' 'real' microtome knives is now sounding reminiscent... "back in the day, I used to..." and likely viewed as a quaint anachronistic ramblings of a well-fixed, but in need of reprocessing, MLT. I am reassured, however, that this discipline still remains relevant to patient care. Fears of being replaced completely by instrumentation have not been realized. With the coming nexus of molecular medicine and pathology, the increased use of ISH and IHC and many other developments I have quietly and humbly witnessed during my long journey to jadedness and grizzledom, I remain optimistic for future Histotechs. Now, where did I leave my, um...where was I? Eric Gagnon MLT Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada From Lacie.Algeo <@t> providence.org Wed Aug 13 17:24:34 2014 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Wed Aug 13 17:25:11 2014 Subject: [Histonet] paraffin Message-ID: <24C4B3C167E5694887AB594C7602CE3A0C2971@WN35104.or.providence.org> Hi all, I am dealing with an issue with slippery floors outside of our Histology department. The concern is that it is from paraffin scraps tracked on the bottoms of peoples shoes. The floor is cleaned every night and an anti-slip agent applied. I have tested whether our paraffin makes the floors more slippery and it actually makes it harder to slip....almost tacky. I really do not think it is our paraffin. I think it is a thin layer of dust that settles on the floors after they are cleaned. I have seen this happen in other places. Is anyone dealing with this? Any insight? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Timothy.Morken <@t> ucsfmedctr.org Wed Aug 13 18:08:41 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Wed Aug 13 18:08:58 2014 Subject: [Histonet] RE: biopsy "bags" for processing - alternatives In-Reply-To: <771255633.1395072.1407959268100.JavaMail.root@comcast.net> References: <771255633.1395072.1407959268100.JavaMail.root@comcast.net> Message-ID: <761E2B5697F795489C8710BCC72141FF3679385A@ex07.net.ucsf.edu> Wow, thanks for all the feedback. Do I get a prize for starting the Topic of the Day? I?ll look in to all these suggestions and hopefully make everyone happy (riiiigghhhtt?) Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA From: Pam Marcum [mailto:mucram11@comcast.net] Sent: Wednesday, August 13, 2014 12:48 PM To: Sally Cc: Tom McNemar; Morken, Timothy; Histonet Subject: Re: [Histonet] RE: biopsy "bags" for processing - alternatives If we grossed them we could control this. However; we are physically a quarter mile ftom the Gross Room so we get what we get and have learned to live with it. I have used lens paper in the past and it is not used here. I also had more say in how the biopsies were handled and by whom than I have here so I just look for better ways that we might get approved. Pam ________________________________ From: "Sally" > To: "Tom McNemar" >, "Timothy' 'Morken" >, "Histonet" > Sent: Wednesday, August 13, 2014 2:17:39 PM Subject: RE: [Histonet] RE: biopsy "bags" for processing - alternatives I always used lens paper. Wrap paper over tissue at an oblique angle so the forceps can grab a corner of the thin paper and peel away without losing tissue - whether hot or cold paraffin. Never lost a tissue... -----Original Message----- From: "Tom McNemar" > Sent: ?8/?13/?2014 9:59 AM To: "'Morken, Timothy'" >; "Histonet" > Subject: [Histonet] RE: biopsy "bags" for processing - alternatives Personally, I prefer the biopsy bags and find them easy to work with. I generally hold the bag in one hand (allows the paraffin to cool enough to hold the tissue) and use forceps to slowly open it. If it is an ECC or something similar, I can wrap the opened bag around a couple of fingers and use a small spatula to scrap. With a little practice, I have found it to be quick and efficient. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, August 13, 2014 11:24 AM To: Histonet Subject: [Histonet] biopsy "bags" for processing - alternatives All knowing Histonet, Our grossing staff uses nylon "biopsy bags" to enclose some biopsy specimens. The embedding staff find them troublesome because when they pull the bags open they tend to "pop" open and throw the tissue off in all directions. They have to be very careful opening these. Is there another bag made of some other material that is less prone to this problem? For various reasons some of these samples can't be put on sponges. They do wrap some in flat biopsy paper, but not others. It seems to be a grossing personal preference more than anything else. Thanks for any and all info! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Wed Aug 13 20:05:43 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Wed Aug 13 20:05:50 2014 Subject: [Histonet] RE: paraffin In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A0C2971@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A0C2971@WN35104.or.providence.org> Message-ID: <0B8979A204680A42B93A52B486088CD93938636ADA@CUAEXH1.GCU-MD.local> You may want to purchase the large tack mats for staff to walk over when going in and out of your embedding area. These come in blue, white, or gray: http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=29104&productId=5264212&distype=0&highlightProductsItemsFlag=Y&fromSearch=1&searchType=PROD&hasPromo=0 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A [Lacie.Algeo@providence.org] Sent: Wednesday, August 13, 2014 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin Hi all, I am dealing with an issue with slippery floors outside of our Histology department. The concern is that it is from paraffin scraps tracked on the bottoms of peoples shoes. The floor is cleaned every night and an anti-slip agent applied. I have tested whether our paraffin makes the floors more slippery and it actually makes it harder to slip....almost tacky. I really do not think it is our paraffin. I think it is a thin layer of dust that settles on the floors after they are cleaned. I have seen this happen in other places. Is anyone dealing with this? Any insight? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From epeters2 <@t> gmu.edu Wed Aug 13 20:17:43 2014 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Wed Aug 13 20:18:03 2014 Subject: [Histonet] RE: paraffin In-Reply-To: <0B8979A204680A42B93A52B486088CD93938636ADA@CUAEXH1.GCU-MD.local> References: <24C4B3C167E5694887AB594C7602CE3A0C2971@WN35104.or.providence.org>, <0B8979A204680A42B93A52B486088CD93938636ADA@CUAEXH1.GCU-MD.local> Message-ID: <1407979089877.16536@gmu.edu> The nice thing about those is that "sticky mats" will also collect the dust from shoes; I found that these stop a lot of dust coming in at the outside door entrances, as well as having them to collect paraffin under the embedding center, in front of the tissue processor and fume hoods, and under the microtomes. It is amazing how much dust and dirt can come in with people's shoes! Esther C. Peters, Ph.D. Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Walter Benton Sent: Wednesday, August 13, 2014 9:05 PM To: Algeo, Lacie A; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: paraffin You may want to purchase the large tack mats for staff to walk over when going in and out of your embedding area. These come in blue, white, or gray: http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=29104&productId=5264212&distype=0&highlightProductsItemsFlag=Y&fromSearch=1&searchType=PROD&hasPromo=0 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A [Lacie.Algeo@providence.org] Sent: Wednesday, August 13, 2014 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin Hi all, I am dealing with an issue with slippery floors outside of our Histology department. The concern is that it is from paraffin scraps tracked on the bottoms of peoples shoes. The floor is cleaned every night and an anti-slip agent applied. I have tested whether our paraffin makes the floors more slippery and it actually makes it harder to slip....almost tacky. I really do not think it is our paraffin. I think it is a thin layer of dust that settles on the floors after they are cleaned. I have seen this happen in other places. Is anyone dealing with this? Any insight? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raghulj <@t> orchidpharma.com Thu Aug 14 00:29:22 2014 From: raghulj <@t> orchidpharma.com (raghulj@orchidpharma.com) Date: Thu Aug 14 00:31:18 2014 Subject: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18 In-Reply-To: <001001cfb704$c0a1b8d0$41e52a70$@bresnan.net> References: <52ee1248-6af4-4f0b-b495-e764619dcfc5@DB3FFO11FD027.protection.gbl> <001001cfb704$c0a1b8d0$41e52a70$@bresnan.net> Message-ID: Hi Gayle, I do get your view on making a judgement. We do our own issues at times, in microtomy/staining. Nicely elaborated.I would like to go through the publication to have a better understanding. Pl. do share to my email- raghulj@orchidpharma.com. Thanks, raghul -----Original Message----- From: gayle callis [mailto:gayle.callis@bresnan.net] Sent: 13 August 2014 20:12 To: Raghul J (Biology); histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18 The best way to determine the final endpoint is radiography as it is the most sensitive if you have a FAXITRON. If you have this xray machine or a microCT, then you should use these for decalcification endpoint and not waste time with weight loss/weight gain method or any other method. When we do the weight loss/weight gain, we simply made the judgement call that the weight gain means no calcium is present and no further testing is required. It is considered bad practice to "prick" the bone as you may 1) create needle tract artifact into critical areas you might want to see 2) possibly dislodge a small pathological lesion from the bone 3) bending bone can dislodge tumor from bone . Consequently, we never did mechanical testing EVER! We would decalcify as many as 40 to 50 samples - a collection of NBF fixed mouse knees, or tibias, or paws at one time in a large 1 to 2 liters 10% to 15% formic acid. Having to weigh each and every one of these small bones was tedious and time consuming. We found that if we took representative samples of each kind of bone - 4 from experimental group, and 4 from control group and use these bones as the wt loss/wt gain samples, the remaining samples still decalcifying were so close in size (and weight), we had excellent decalcification without damaging the bone. One learns very quickly which murine leg bones decalcify faster than other with paws taking much more time due to the tiny bones so tightly packed together. The original Mawhinney et al weight gain/weight loss publication tested the last nitric acid change with a chemical test. Unfortunately, doing chemical testing with EDTA is even more tedious. Some people will actually let the bones sit a few hours longer in formic acid decalcifiers to ensure the calcium is removed but you may over expose and do acid hydrolysis the antigens, nuclei i.e. "overdecalcification" . You can do this but I suggest testing the bones again so see if that weight gain has increased or not. If not, then you may have left the bones in acid too long. It doesn't take long to damage staining with acid exposure. A chemical endpoint test is more accurate than weight loss/weight gain, but you can't be doing a huge number of samples in one container of decalcifying solution. This is probably the reason those authors did chemical test after weight loss/weight gain but with single samples. I know of one person who success doing chemical test for 4 to 5 bone slabs of same size and approximate weight, decalcfified in an acid solution, and did the chemical test from a single container of used, never stirred decalcifier for those 4 or 5 large samples. I will be happy to send the original publication to you via private email, and the chemical test method. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghulj@orchidpharma.com Sent: Tuesday, August 12, 2014 10:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129, Issue 18 Dear all, In the weight loss/gain method how would we fix the optimal endpoint to stop the decal process based on weight gain? Will weighing omit the other ways of judgement such as needle prick, physical examination. In our tox experiments we deal with lot of femurs in one stretch and weighing method looks interesting. Pl. comment. J.Raghul Senior Research Associate Orchid Chemicals and Pharamaceuticals Limited Toxicology, Chennai,India. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 12 August 2014 22:38 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: On the lighter side... (Shirley A. Powell) 2. Bcl-2 (Heather Knight) 3. IHC Steam Strips: (Jb) 4. RE: Are Paraffin Blocks Biohazard (Michael LaFriniere) 5. RE: On the lighter side... (Mark Turner) 6. RE: On the lighter side... (Bernice Frederick) 7. Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 8. Re: Weight Loss/Weight Gain Decal (Jennifer MacDonald) 9. RE: Weight Loss/Weight Gain Decal (Wait, Trevor Jordan) 10. RE: Histonet Digest, Vol 129, Issue 17 (Mayer,Toysha N) 11. RE:On the lighter side... (Mayer,Toysha N) 12. Re: RE:On the lighter side... (Barry Rittman) 13. Re: Weight Loss/Weight Gain decalcification endpoint test (gayle callis) 14. Consult for taking abs from RUO to IVD (Patsy Ruegg) 15. RE: On the lighter side... (Susan.Walzer@HCAHealthcare.com) 16. RE: Cytospin validation (Jamal) 17. Re: On the lighter side... (Michael Ann Jones) 18. looking for TS replacement in Histology due to retirement (Webb, Dorothy L) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Aug 2014 13:03:38 -0400 From: "Shirley A. Powell" Subject: RE: [Histonet] On the lighter side... To: Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <9BF995BC0E47744E9673A41486E24EE25BFB25FD10@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="iso-8859-1" I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 11 Aug 2014 13:18:57 -0400 From: Heather Knight Subject: [Histonet] Bcl-2 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Hi everyone- Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse tissue? If so, can you share both the protocol and the antibody information? We have tried numerous antibodies over the years with very limited success. Thank you for your help!! Best, Heather Knight ------------------------------ Message: 3 Date: Mon, 11 Aug 2014 10:18:53 -0700 From: Jb Subject: [Histonet] IHC Steam Strips: To: Histonet@lists.utsouthwestern.edu Message-ID: <375F4EF9-47B6-46CD-B5B1-B511309EF4AA@gmail.com> Content-Type: text/plain; charset=us-ascii Does anyone use IHC steam strips in their decloaking chamber? The question is do you run a new steam strip each run and log each individual run w/it's own steam strip? Thank you, Sent from my iPhone ------------------------------ Message: 4 Date: Mon, 11 Aug 2014 17:43:34 +0000 From: Michael LaFriniere Subject: RE: [Histonet] Are Paraffin Blocks Biohazard To: Dawn Bugge , "histonet@lists.utsouthwestern.edu" Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D296A0A37@AHCMSASEXCH00.my.ahc.local> Content-Type: text/plain; charset="us-ascii" Dawn, The only study I know of is on CJD crutsfeldt-Jacobs Disease (know to survive formalin fixation and routine processing protocols, the CDC web site has additional information, In my laboratories I put all blocks in hazardous waste for incineration disposal. It is not that costly just to be on the safe side. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge Sent: Friday, August 08, 2014 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are Paraffin Blocks Biohazard Hello Histo World! Our pathologist for our private GI lab would like me to find out if anyone has done a study to determine if the paraffin blocks, once they have been processed, are considered biohazard. I have searched high and low and can find many people stating that the blocks are not bioharzard, with the exception of neurological tissue, but they don't state how they know this. He would like me to reference an actual study to prove that someone has actually looked into this. Any one know of something like this? I know common sense would say that once the tissues have been in formalin for hours, than on the processor for hours that the tissue would be non biohazard and completely safe. Thanks for your help :) -- Dawn R Bugge HT(ASCP), Lab Manager Seattle Histology Dawns Usborne Books Website _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 11 Aug 2014 17:49:13 +0000 From: Mark Turner Subject: RE: [Histonet] On the lighter side... To: "Shirley A. Powell" , Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <643626B74DE2814D8537057F40E1A10B0CAD409D@CSI-MX-NODEA.CSI-LABS.local> Content-Type: text/plain; charset="iso-8859-1" Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 11 Aug 2014 17:53:31 +0000 From: Bernice Frederick Subject: RE: [Histonet] On the lighter side... To: Mark Turner , "Shirley A. Powell" , Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F0F3167@evcspmbx2.ads.northwestern.edu> Content-Type: text/plain; charset="iso-8859-1" Or we'll live forever..... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Monday, August 11, 2014 12:49 PM To: Shirley A. Powell; Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Saves on embalming costs.... Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Monday, August 11, 2014 1:04 PM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... I agree. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 11 Aug 2014 19:26:57 +0000 From: "Wait, Trevor Jordan" Subject: [Histonet] Weight Loss/Weight Gain Decal To: "histonet@lists.utsouthwestern.edu" Message-ID: <1407785218488.59806@livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ------------------------------ Message: 8 Date: Mon, 11 Aug 2014 12:33:51 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] Weight Loss/Weight Gain Decal To: "Wait, Trevor Jordan" Cc: "histonet@lists.utsouthwestern.edu" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" To: "histonet@lists.utsouthwestern.edu" Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces@lists.utsouthwestern.edu Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 11 Aug 2014 19:35:29 +0000 From: "Wait, Trevor Jordan" Subject: RE: [Histonet] Weight Loss/Weight Gain Decal To: Jennifer MacDonald Cc: "histonet@lists.utsouthwestern.edu" , "histonet-bounces@lists.utsouthwestern.edu" Message-ID: <1407785730771.24250@livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" To: "histonet@lists.utsouthwestern.edu" Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces@lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 11 Aug 2014 19:45:01 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 17 To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B4382A0@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Egads, I feel like a baby! 20 years registered, 3 yrs unregistered. By the way, I did see a former colleague with her ASCP certificate in her office, and the oldest sticker is from the year I was born. Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 2 Date: Sun, 10 Aug 2014 21:50:58 +0000 From: Sebree Linda A Subject: RE: [Histonet] On the lighter side... To: Jamal , 'Vincent Rivera' , 'Douglas Porter' , "histonet@lists.utsouthwestern.edu" Message-ID: <77DD817201982748BC67D7960F2F76AF0C7C6D@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" 38 years and looking forward to retirement in a few years. Linda A. Sebree ------------------------------ Message: 11 Date: Mon, 11 Aug 2014 19:52:19 +0000 From: "Mayer,Toysha N" Subject: [Histonet] RE:On the lighter side... To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881B4382E6@D1PWPEXMBX05.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Tim, Just saw your post about the 'marketable skill'. Funny those were my mother's exact words while I was in college. She didn't care what I majored in, as long as I got a marketable skill along the way. The best advice I've ever gotten. Thanks Maria!!! (my mother) Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: 08 August 2014 19:26 To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 1992. Electron Microscopy Technologist, #604, 1982. Like most, I never heard of "histology" until I walked into a hospital lab on my first day as an EM tech. I had seen slides made in college, but no one ever mentioned it could be an actual profession. I was more taken with the electron microscope, and there was (is) a 2-year program at the community college in the town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a marketable skill. At that time EM was still used for tumor dx, so when I started it was about half tumor, half kidney. I was lucky enough to get involved in histology and set up the IHC lab at the small community hospital I worked at (as an EM tech) and so ended up phasing myself almost out of an EM job. The IHC took over all the tumor dx from EM. Later I left EM altogether and did IHC exclusively for 15 years. But, like most, I learned Histotechnology on the job but was lucky enough to work for a pathologist who believed in developing his techs - to the point of paying for meetings out of his own pocket. Only now do I know how fortunate I was to work for someone like that. Because of him we had developed a culture in the small histo lab (4 men!!) of learning. We studied together one night a week for the HT exam and all passed (and the practical!). Again, that was a fortunate experience, not very often seen in labs. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA ------------------------------ Message: 12 Date: Mon, 11 Aug 2014 15:45:05 -0500 From: Barry Rittman Subject: Re: [Histonet] RE:On the lighter side... To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Well fixed in the histological sense I hope. Barry On Mon, Aug 11, 2014 at 2:52 PM, Mayer,Toysha N wrote: > Tim, > > Just saw your post about the 'marketable skill'. Funny those were my > mother's exact words while I was in college. She didn't care what I > majored in, as long as I got a marketable skill along the way. > The best advice I've ever gotten. > Thanks Maria!!! (my mother) > > Sincerely, > > Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education > Coordinator Program in Histotechnology School of Health Professions UT > M.D. Anderson Cancer Center > 713.563-3481 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: 08 August 2014 19:26 > To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] On the lighter side... > > Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, > 1992. Electron Microscopy Technologist, #604, 1982. > > Like most, I never heard of "histology" until I walked into a hospital lab > on my first day as an EM tech. I had seen slides made in college, but no > one ever mentioned it could be an actual profession. I was more taken with > the electron microscope, and there was (is) a 2-year program at the > community college in the town I grew up in (Delta College, Stockton, CA). > So AFTER getting a BA in Zoology, I went there to get a marketable skill. > At that time EM was still used for tumor dx, so when I started it was about > half tumor, half kidney. I was lucky enough to get involved in histology > and set up the IHC lab at the small community hospital I worked at (as an > EM tech) and so ended up phasing myself almost out of an EM job. The IHC > took over all the tumor dx from EM. Later I left EM altogether and did IHC > exclusively for 15 years. But, like most, I learned Histotechnology on the > job but was lucky enough to work for a pathologist who believed in > developing his techs - to the point of paying for meetings out of his own > pocket. Only now do I know how fortunate I was to work for someone like > that. Because of him we had developed a culture in the small histo lab (4 > men!!) of learning. We studied together one night a week for the HT exam > and all passed (and the practical!). Again, that was a fortunate > experience, not very often seen in labs. > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies UC San Francisco Medical Center San Francisco, CA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Mon, 11 Aug 2014 15:35:09 -0600 From: "gayle callis" Subject: [Histonet] Re: Weight Loss/Weight Gain decalcification endpoint test To: Message-ID: <000601cfb5ac$22447d70$66cd7850$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Trevor and Jennifer, This method originally came from Mawhinney et al. Control of rapid nitric acid decalcification J Clin Pathol 1984 37:1409-1415 and was cited by Cathy Sanderson (Mayton)in a publication using EDTA, found in Biotechnic and Histochemistry. Mawhinney acutally did a chemical test on final acid change to see that calcium was not present, but we never had to do that. If you end up with a bit of residual calcium in block, I would surface decalcify at microtomy. I used it for years when we downsized and gave away our FAXITRON. Radiography is still the most accurate, and if you had either a micro CT or digital FAXITRON available, it would be a better test. I used Cathy's method and will be happy to send the original publication for anyone's files/future referencing. A chemical test for EDTA is a pain to do if a FAXITRON is not available. I have put the full method below, with a bit more detail. WEIGHT LOSS/WEIGHT GAIN ENDPOINT TEST This is a method of choice for EDTA decalcification although it was originally used by Mawhinney et al for testing nitric acid decalcification. Many samples can be decalcified together in one container, i.e. 25 mouse femurs in 1 liter of 10% formic acid. If all the samples are the same size, i.e. mouse femurs, tibia, paws, choose several as representative samples and test only those to save time. Always suspend bones in the decalcifier. Requires a balance that reads in milligrams to 3 places for greatest accuracy. Specimen must be blotted free of fluid for accurate weighing each time you weigh the sample. We suspend bones in nylon specimen bags for easy removal to weigh. Bags can be marked with pencil too. Technique: 1. Rinse NBF off bone, blot with paper towel, WEIGH BONE, RECORD BEGINNING WEIGHT. Suspend bone in acid or EDTA decalcifier. During acid decalcification CO2 bubbles are given off, so stir during decalcification to release bubbles or small samples will float. EDTA does not create CO2 bubbles, only acids. Large bones can be started at end of day in acid decalcifier and sit overnight with testing the next morning. 2. After 4 to 5 hours in acid or overnight in EDTA, remove bone, rinse with water, BLOT, weigh. RECORD WEIGHT. If bone shows loss of weight, change acid decalcifier to refresh acid. Return samples to resume decalcification, and repeat as many times as necessary. EDTA should be changed but not as often as acid. Always use a large volume of decalcifier i.e. 20:1 or more. Remove bone from specimen bag, and place in weighing boat to protect balance from acids/EDTA. 3. When bone begins to GAIN WEIGHT, the bone is decalcified. Once calcium is removed, water is taken on and the weight increases. This water does not affect the bone. 4. Rinse bone with running tap water for an hour or longer to remove these decalcifiers. Either store in 70% alcohol or process. Store endpoint tested decalcified bones in 70% alcohol while waiting for other samples to finish decalcifying and mass processing run. Reminders: For EDTA, one can suspend bones and check every day for accuracy but bones can be left in the EDTA over a weekend or several days without damage as long as the bones were well fixed. Acid decalcified bones cannot be left over a weekend, remove from acid, put in NBF to stop decalcification. Bones should be endpoint tested before stopping decalcification so you can resume decalcification on the next working day. Rinse off NBF briefly before resuming decalcification. Do not overexpose bones to acids or you will damage antigens and nuclear staining. Enjoy the method, as it truly is fast and easy. Gayle M. Callis HTL/HT/MT(ASCP) ____________________________________________________________________________ _____ Ha Wow...that's almost too easy. Thank you for this! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ________________________________ From: Jennifer MacDonald < JMacDonald <@t> mtsac.edu> Sent: Monday, August 11, 2014 2:33 PM To: Wait, Trevor Jordan Cc: histonet <@t> lists.utsouthwestern.edu; histonet-bounces <@t> lists.utsouthwestern.edu Subject: Re: [Histonet] Weight Loss/Weight Gain Decal I believe this was originally from Patsy Ruegg Decalcification End Point: Weight Loss, Weight Gain 1. Blot sample to remove excess fixative 2. Weigh bone in mg, record as beginning weight 3. Next day, rinse bone, blot and weigh bone daily, record weight. Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh decalcifying solution. 4. When bone begins to GAIN weight, remove from decalcifying solution, rinse and process. YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY Once calcium is totally removed (bone loses weight as this happens), water replaces the calcium and weight begins to go up. This is the point at which calcium should be totally gone. The original method used a chemical test at the end to insure no calcium was in the decalcifying solution. If you do this, you cannot stir the solution during decalcification. Be sure to suspend bone in the solution to insure all sides of bone are in contact with decalcification solution. From: "Wait, Trevor Jordan" < WaitT <@t> livemail.uthscsa.edu> To: " histonet <@t> lists.utsouthwestern.edu" < histonet <@t> lists.utsouthwestern.edu> Date: 08/11/2014 12:29 PM Subject: [Histonet] Weight Loss/Weight Gain Decal Sent by: histonet-bounces <@t> lists.utsouthwestern.edu ________________________________ Hello all! I'm currently doing some decalcification and was curious if anyone had some particular advice about the weight loss/weight gain method. I understand that when the decalcification process is complete, the tissue block will begin to increase in weight. However, I'm confused when I should record the weight for the block once they have been taken out of the EDTA solution. You see, for the times I weighed the blocks before... the weights were a little skewed because there were differing amounts of solution on the blocks while they were sitting on the balance. I just want to standardize my protocol a little more so that I can be sure the block is actually gaining weight due to the calcium loss rather than just extra solution sitting on the outside of the block. Would letting the blocks sit out of solution for about 30 minutes before being weighed help with the matter? I know that the blocks take on water once they are completely decalcified so I'm not sure how much this will affect that. Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry _______________________________________________ Histonet mailing list Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 11 Aug 2014 17:04:32 -0600 From: Patsy Ruegg Subject: [Histonet] Consult for taking abs from RUO to IVD To: "Histonet@Lists. Edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Colleagues, I am consulting with an antibody vendor who needs a consultant to help them take their abs from RUO to IVD. If you know anyone who does this type of work you could recommend would you please have them contact me directly and I will pass their contact info on. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net ------------------------------ Message: 15 Date: Tue, 12 Aug 2014 01:58:14 -0500 From: Subject: RE: [Histonet] On the lighter side... To: , Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FBFFC425D@FWDCWPMSGCMS09.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" Also heard" Histotechs are good embed!!!" LOL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Monday, August 11, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Old histologists never die, they are just well fixed... Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjones@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwards, Richard; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] On the lighter side... 25 years, ("what?s that in micron?s??) Bernice, you are too funny!! (lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: >Sniffed my first formalin and saw first post-mortem November 1965. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 12 Aug 2014 10:49:24 +0300 From: "Jamal" Subject: [Histonet] RE: Cytospin validation To: Message-ID: <013e01cfb601$f0da61e0$d28f25a0$@rowaihi@alborglaboratories.com> Content-Type: text/plain; charset="us-ascii" Good day colleagues Ignoring my previous message what it means: no one did validation for Cytospin !! or no one want to share his information ?? or no one received my message !!?? Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From: Jamal [mailto:j.rowaihi@alborglaboratories.com] Sent: Monday, August 11, 2014 1:47 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Cytospin validation Hi I need to do validation for my Shandon Cytospin 4, can anyone send me the validation procedure and form. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com ------------------------------ Message: 17 Date: Tue, 12 Aug 2014 13:00:08 +0000 From: Michael Ann Jones Subject: Re: [Histonet] On the lighter side... To: "Susan.Walzer@HCAHealthcare.com" , "CIngles@uwhealth.org" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="euc-kr" Ha ha!! Good one - Michael Ann On 8/12/14, 12:58 AM, "Susan.Walzer@HCAHealthcare.com" wrote: >Also heard" Histotechs are good embed!!!" LOL > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles >Claire >Sent: Monday, August 11, 2014 12:00 PM >To: histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] On the lighter side... > > >Old histologists never die, they are just well fixed... >Claire >________________________________________ >From: histonet-bounces@lists.utsouthwestern.edu >[histonet-bounces@lists.utsouthwestern.edu] on behalf of Michael Ann >Jones [mjones@metropath.com] >Sent: Monday, August 11, 2014 9:07 AM >To: Edwards, Richard; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] On the lighter side... > >25 years, ("what??s that in micron??s???) Bernice, you are too funny!! >(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) >Histology Manager Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com > > > > >On 8/11/14, 5:17 AM, "Edwards, Richard" wrote: > >>Sniffed my first formalin and saw first post-mortem November 1965. >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 12 Aug 2014 11:18:12 -0500 From: "Webb, Dorothy L" Subject: [Histonet] looking for TS replacement in Histology due to retirement To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <65365F35C0F2EF4D846EC3CA73E49C4302F6D319B813@HPEMX3.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Histology Technical Specialist Position in St. Paul, MN Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and "Best Care, Best Experience", has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will oversee the technical component of a work team in the histology laboratory including; implementation of methodologies, supply ordering and inventory management, interpretation of laboratory results for physicians and other hospital staff; performance of laboratory tests in the section assigned; assisting in performance evaluations; assisting in supervision of testing personnel; development and implementation of training and competency programs for histotechnicians and histology students interns, and to perform related duties as assigned. Qualifications: Graduation from an accredited college or university with an Associates (AA) or bachelor's degree in medical technology, histology, biology or related field; plus specialized training in laboratory science. A master's degree in related field may substitute for the above educational and registration requirements. Bachelor's degree preferred. At least four (4) years of experience as a Histotechnician or Histotechnologist or related field with at least two (2) years of experience in the specialty occurring within the last four years. How to Apply Apply online at www.regionshospital.com Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 18 ***************************************** THIS E-MAIL IS CONFIDENTIAL and intended solely for the use of the individual to whom it is addressed. If you are not the addressee, any disclosure, reproduction, copying, distribution, or other dissemination or use of this communication is strictly prohibited. Finally the recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS E-MAIL IS CONFIDENTIAL and intended solely for the use of the individual to whom it is addressed. If you are not the addressee, any disclosure, reproduction, copying, distribution, or other dissemination or use of this communication is strictly prohibited. Finally the recipient should check this email and any attachments for the presence of viruses. The company accepts no liability for any damage caused by any virus transmitted by this email From jmoreira <@t> sidra.org Thu Aug 14 00:39:09 2014 From: jmoreira <@t> sidra.org (Joana Moreira) Date: Thu Aug 14 00:39:43 2014 Subject: [Histonet] Cerner Millennium AP Module + AB&T and Cerner Millenium AP Module + Cerebro Message-ID: Dear all, I have posted this before but am trying my luck once more. I am trying to find out if there's any AP lab currently using (or looking into it) the AP module of Cerner Millennium LIS system with their specimen tracking system AB&T (Advanced Barcode and Tracking) and also if there's anyone using the Cerner Millennium AP Module with Leica's Cerebro Specimen Tracking system. If you do and would be willing to discuss / share experiences, etc please personal message me jmoreira@sidra.org Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira@sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From Susan.Walzer <@t> HCAHealthcare.com Thu Aug 14 02:08:08 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Thu Aug 14 02:08:15 2014 Subject: [Histonet] biopsy "bags" for processing - alternatives In-Reply-To: <1259267934.918388.1407950260092.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> References: <761E2B5697F795489C8710BCC72141FF36793626@ex07.net.ucsf.edu> <1259267934.918388.1407950260092.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FC012DBA0@FWDCWPMSGCMS09.hca.corpad.net> When you open nylon bags they sometime "pop" the biopsy out and you can lose it. Tiny tissue gets stuck in the the seams of the bags. Blue sponges made artifact indents in tissue. We use round Obex papers and have never lost a biopsy. They are wonderful! You can fold them like filter paper ,use a small funnel and pour tiny bits of tissue thru catching everything. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Wednesday, August 13, 2014 1:18 PM To: Timothy Morken Cc: Histonet Subject: Re: [Histonet] biopsy "bags" for processing - alternatives We also use the nylon bags and have to pay extreme attention when embedding.? We no longer use sponges due to cross contamaination.? We use to buy tissue bags from Fisher and they were similar to the "blue" paper we use from Leica.? I have been looking at tea bag vendors (like we get Lipton and Salda tea in).? They are paper the only issue is the quantity you have to purchase.? Mopec make a bag but the issue the open end is even and this is a real pain when trying to get inside, as I mentioned before the old Fisher bags had one end longer than the other so you were able to place your forceps inside with ease.? Another reason I would like to replace the nylon bag is that when you get an aggregate of tissue some of it gets inside the mesh and if there is minimal tissue I fear you may miss some diagnostic material. Sue PaturzoTJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Aug 14 11:02:41 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Thu Aug 14 11:02:55 2014 Subject: [Histonet] RE: paraffin In-Reply-To: <1407979089877.16536@gmu.edu> References: <24C4B3C167E5694887AB594C7602CE3A0C2971@WN35104.or.providence.org>, <0B8979A204680A42B93A52B486088CD93938636ADA@CUAEXH1.GCU-MD.local> <1407979089877.16536@gmu.edu> Message-ID: <002401cfb7d9$2f550140$8dff03c0$@bresnan.net> I want these in doorways of my house. We used tacky mats at entrance of designated room for prion tissue work i.e. paraffin microtomy and cryomicrotomy, etc. Fabulous way to keep the floors clean. We also had industrial rugs i.e. the kind seen at entrances to large buildings in lab where routine paraffin microtomy was done. These were vacuumed daily by the janitorial staff and could be shampooed if needed. These rugs were put in after a visiting professor slipped on paraffin slick, painted concrete floor. He would have bashed back of his head but managed to catch himself on way down, clutching a door knob. I think the rugs avoid potential injuries and lawsuits against our university. Go for the sticky mats!!! I liked how Esther used them in all areas, and certainly better than rugs which are eventually trashed and unsightly with ground in paraffin residue. I suggest in front of the processors too. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Esther C Peters Sent: Wednesday, August 13, 2014 7:18 PM To: Walter Benton; Algeo, Lacie A; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: paraffin The nice thing about those is that "sticky mats" will also collect the dust from shoes; I found that these stop a lot of dust coming in at the outside door entrances, as well as having them to collect paraffin under the embedding center, in front of the tissue processor and fume hoods, and under the microtomes. It is amazing how much dust and dirt can come in with people's shoes! Esther C. Peters, Ph.D. Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZ NdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.ed u ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Walter Benton Sent: Wednesday, August 13, 2014 9:05 PM To: Algeo, Lacie A; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: paraffin You may want to purchase the large tack mats for staff to walk over when going in and out of your embedding area. These come in blue, white, or gray: http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&ca talogId=29104&productId=5264212&distype=0&highlightProductsItemsFlag=Y&fromS earch=1&searchType=PROD&hasPromo=0 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A [Lacie.Algeo@providence.org] Sent: Wednesday, August 13, 2014 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin Hi all, I am dealing with an issue with slippery floors outside of our Histology department. The concern is that it is from paraffin scraps tracked on the bottoms of peoples shoes. The floor is cleaned every night and an anti-slip agent applied. I have tested whether our paraffin makes the floors more slippery and it actually makes it harder to slip....almost tacky. I really do not think it is our paraffin. I think it is a thin layer of dust that settles on the floors after they are cleaned. I have seen this happen in other places. Is anyone dealing with this? Any insight? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Thu Aug 14 12:47:52 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Thu Aug 14 12:47:59 2014 Subject: [Histonet] RE: paraffin In-Reply-To: <002401cfb7d9$2f550140$8dff03c0$@bresnan.net> References: <24C4B3C167E5694887AB594C7602CE3A0C2971@WN35104.or.providence.org> <0B8979A204680A42B93A52B486088CD93938636ADA@CUAEXH1.GCU-MD.local> <1407979089877.16536@gmu.edu> <002401cfb7d9$2f550140$8dff03c0$@bresnan.net> Message-ID: New (or unparaffined, to coin a term) shoes are the major threat here, imho. When one has paraffin on the bottom of one's shoes and steps on more paraffin, it is kind of sticky. But if you wear new shoes, or shoes that have never been in the histo lab, look out! Hence Gayle's experience with the visiting professor, and I have similar stories. It's usually visitors that slip. Large, graphic, signs outside of the histo lab also help to warn visitors. On Thu, Aug 14, 2014 at 11:02 AM, gayle callis wrote: > I want these in doorways of my house. We used tacky mats at entrance of > designated room for prion tissue work i.e. paraffin microtomy and > cryomicrotomy, etc. Fabulous way to keep the floors clean. > > We also had industrial rugs i.e. the kind seen at entrances to large > buildings in lab where routine paraffin microtomy was done. These were > vacuumed daily by the janitorial staff and could be shampooed if needed. > These rugs were put in after a visiting professor slipped on paraffin > slick, painted concrete floor. He would have bashed back of his head > but managed to catch himself on way down, clutching a door knob. I think > the rugs avoid potential injuries and lawsuits against our university. > > Go for the sticky mats!!! I liked how Esther used them in all areas, and > certainly better than rugs which are eventually trashed and unsightly with > ground in paraffin residue. I suggest in front of the processors too. > > Gayle Callis > HTL/HT/MT(ASCP) > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Esther C > Peters > Sent: Wednesday, August 13, 2014 7:18 PM > To: Walter Benton; Algeo, Lacie A; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: paraffin > > The nice thing about those is that "sticky mats" will also collect the dust > from shoes; I found that these stop a lot of dust coming in at the outside > door entrances, as well as having them to collect paraffin under the > embedding center, in front of the tissue processor and fume hoods, and > under > the microtomes. It is amazing how much dust and dirt can come in with > people's shoes! > > Esther C. Peters, Ph.D. > Associate Professor > Environmental Science & Policy > George Mason University > 4400 University Drive, MS 5F2 > Fairfax, VA 22030-4444 > Office: David King Hall, Room 3050 > Phone: 703-993-3462 > Fax: 703-993-1066 > e-mail: epeters2@gmu.edu > > https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZ > > NdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.ed > u > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > on behalf of Walter Benton > > Sent: Wednesday, August 13, 2014 9:05 PM > To: Algeo, Lacie A; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: paraffin > > You may want to purchase the large tack mats for staff to walk over when > going in and out of your embedding area. These come in blue, white, or > gray: > > http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&ca > > talogId=29104&productId=5264212&distype=0&highlightProductsItemsFlag=Y&fromS > earch=1&searchType=PROD&hasPromo=0 > > > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > Chesapeakeurology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A > [Lacie.Algeo@providence.org] > Sent: Wednesday, August 13, 2014 6:24 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] paraffin > > Hi all, > I am dealing with an issue with slippery floors outside of our Histology > department. The concern is that it is from paraffin scraps tracked on the > bottoms of peoples shoes. The floor is cleaned every night and an > anti-slip > agent applied. I have tested whether our paraffin makes the floors more > slippery and it actually makes it harder to slip....almost tacky. I really > do not think it is our paraffin. I think it is a thin layer of dust that > settles on the floors after they are cleaned. I have seen this happen in > other places. Is anyone dealing with this? Any insight? > Thanks, > Lacie > > Lacie Algeo, HTL (ASCP) MBCM > Histology Supervisor > Providence Sacred Heart Medical Center Laboratory > 101 W 8th Avenue > L-2 > Spokane, WA 99204 > 509-474-4418 > FAX 509-474-2052 > lacie.algeo@providence.org > > > This message is intended for the sole use of the addressee, and may contain > information that is priviledged, confidential and exempt from disclosure > under applicable law. If you are not the addressee, you are hereby > notified > that you may not use, copy, disclose or distribute to anyone the message or > any information contained in the message. If you have received this > message > in error, please immediately advise the sender by reply e-mail and delete > this message. > > > ________________________________ > > This message is intended for the sole use of the addressee, and may contain > information that is privileged, confidential and exempt from disclosure > under applicable law. If you are not the addressee you are hereby notified > that you may not use, copy, disclose, or distribute to anyone the message > or > any information contained in the message. If you have received this message > in error, please immediately advise the sender by reply email and delete > this message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: The information contained in this electronic > message > is intended solely for the personal and confidential use of the designated > recipient(s) named above and may contain information that is protected from > disclosure under applicable law. If you are not the intended recipient, or > the employee or agent responsible for delivering it to the intended > recipient, you are hereby notified that any dissemination, distribution or > copying of this transmission is strictly prohibited. If you have received > this transmission in error, please notify the transmitting > person/department > immediately by email or telephone (410) 581-5881 and delete the message > without making a copy. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jennifer.Johnson <@t> genzyme.com Thu Aug 14 14:43:47 2014 From: Jennifer.Johnson <@t> genzyme.com (Jennifer.Johnson@genzyme.com) Date: Thu Aug 14 14:43:57 2014 Subject: [Histonet] biopsy "bags" for processing - alternatives Message-ID: <0C0766AB928A0E4DB4760C0DB41F91B984A312BB@XSPW10A507T.pharma.aventis.com> Hi, We have a two alternatives to biopsy bags. I work with mouse tissues, but I bet you could probably adapt them to human biopsy applications. The first is the trifold paper towels (you know, the off white rough paper towels you pull from a dispenser in the ladies room). The nice thing is that they are usually in every necropsy room or lab I have had to work in. I used them out of desperation once (necessity is not the ONLY mother of invention!). Now it is what I choose to use every time. Before starting a necropsy will cut up the a few of the paper towels into either long skinny rectangles or squares about twice the size of the cassettes. I use them 2 ways - for tissues that are small, which is what your needs are here, I will put the sample in the middle and fold the towel around it. I fold it the same way every time so that the embedders know how to open my little pockets. A nice little additional advantage is that the tissue is a little damp and the towel is dry so it is easy to orient the samples if they are small and stringy (mouse sciatic nerves) especially because the paper towels are just a little rough. The other way I use the paper towel is the rectangular strip. I use it to keep tissues flat by gently spreading out a piece of rodent skin onto one end and then folding the other end of the paper towel over it. It holds it flat during fixation so that when it comes out of the processor, it is easily lifted off and oriented. The paper towels are porous so they allow the processing fluids to penetrate easily. They carry over less contaminating fluids in the processors than the sponges too. Best of all, they are cheap and easily found! I love them. Our other alternative is Cell Safe cassettes. I am not sure who makes them but I know that may distributors sell them. I usually get them from EMS. Hope this helps, Jenn Jennifer Johnson Staff Scientist Genzyme Corp. Department of Pathology 5 Mountain Road Framingham, MA 01701-9322 From delsuec <@t> gmail.com Thu Aug 14 15:47:36 2014 From: delsuec <@t> gmail.com (Deloris Carter) Date: Thu Aug 14 15:47:39 2014 Subject: [Histonet] Apology Message-ID: My gmail account has been compromised. I apologize to anyone who might have received a spam email. Deloris From celebrej <@t> HHSC.CA Fri Aug 15 07:52:48 2014 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Fri Aug 15 07:54:59 2014 Subject: [Histonet] Myophosphorylase Message-ID: <722701352B76E841BFB9EFAF72553CD29CE78284@ipexmailm02.hhsc.ca> Hello Histoland, This is a two part question for all those who do muscles. 1. Is anyone having problems ordering glucose-1-phosphate? 2. Does anyone have a method for myophosphorylase that doesn't require glucose-1-phosphate? Julia Celebre Sr MLT Anatomic Pathology Hamilton Civic Hospitals Hamilton, ON, Canada 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From lcolbert <@t> pathmdlabs.com Fri Aug 15 11:12:54 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Fri Aug 15 11:13:02 2014 Subject: [Histonet] Glassware Cleaning again Message-ID: <12ECD7346266D74691EC2BFC75285E452F4B3BDA@BFL323E10.pathmdlabs.local> Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From wbenton <@t> cua.md Fri Aug 15 11:27:03 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Fri Aug 15 11:28:07 2014 Subject: [Histonet] RE: Glassware Cleaning again In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F4B3BDA@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F4B3BDA@BFL323E10.pathmdlabs.local> Message-ID: <0B8979A204680A42B93A52B486088CD93938636AE7@CUAEXH1.GCU-MD.local> I use this pre-made solution part number 1300-4 from Fisher Sci. http://www.fishersci.com/ecomm/servlet/fsproductdetail?storeId=10652&productId=4845182&catalogId=29104&matchedCatNo=13004&crossRefData=13004%3D1&fromSearch=1&searchKey=4||1300&highlightProductsItemsFlag=Y&endecaSearchQuery=%23store%3DScientific%23nav%3D0%23rpp%3D25%23offSet%3D0%23keyWord%3D1300-4%23searchType%3DPROD%23SWKeyList%3D%5B%5D&xrefPartType=From&savings=0.0&xrefEvent=1408120030713_0&searchType=PROD&hasPromo=0 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert [lcolbert@pathmdlabs.com] Sent: Friday, August 15, 2014 12:12 PM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Glassware Cleaning again Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From bburnett <@t> CapeCodHealth.org Fri Aug 15 11:31:44 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Fri Aug 15 11:32:05 2014 Subject: [Histonet] tissue falling off slides Message-ID: Our lab has encountered an issue with tissue falling off of the slides. It is mostly the patient tissue that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated. Thanks Again, Brandy Burnett, HTL ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From bcooper <@t> chla.usc.edu Fri Aug 15 11:35:41 2014 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Fri Aug 15 11:35:45 2014 Subject: [Histonet] RE: Glassware Cleaning again In-Reply-To: <12ECD7346266D74691EC2BFC75285E452F4B3BDA@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E452F4B3BDA@BFL323E10.pathmdlabs.local> Message-ID: I noticed the discrepancy in spelling too. I looked online for like 30 minutes, and couldn't find anything called "Bromcresol." Found a lot of vendors selling Bromocresol Purple (and Green for that matter). Best I can figure is that this is a typo on CAP's checklist (that's been there for several years now). Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Friday, August 15, 2014 9:13 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Glassware Cleaning again Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From turkekul <@t> gmail.com Fri Aug 15 12:24:29 2014 From: turkekul <@t> gmail.com (Mesru T) Date: Fri Aug 15 12:24:32 2014 Subject: [Histonet] antibody suggestions CD8, FAP, Cd11b, CD11c Message-ID: Dear Histonetters, I need your help to find good antibodies for IHC on mouse and human FFPE tissues like normal tissues and tumors. Please share any information you think might be helpful. CD8 for IHC on mouse FFPE spleen or other lymphoid organs. FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors. CD11b for IHC on mouse FFPE spleen or other lymphoid organs.. CD11c for IHC on mouse FFPE spleen or other lymphoid organs. Thanks in advance for all your help. Regards, Mesru From tbraud <@t> holyredeemer.com Fri Aug 15 12:52:09 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Aug 15 12:52:21 2014 Subject: [Histonet] RE: Tissue falling off In-Reply-To: <20140815161035.DDF801E8060@trendmess-svr.holyredeemer.local> References: <20140815161035.DDF801E8060@trendmess-svr.holyredeemer.local> Message-ID: Hi - Just a couple of suggestions - I've seen this periodically crop up during the years. There are a couple of things to consider: Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol. Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all. We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off. Your water in the waterbaths is clean - Fresh DI water. Once we had a contaminant in our DI water, and the patient tissues slid right off our slides. We use clean slides, good quality water and super clean waterbaths. Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers. We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle. We now send a rack through first, which turns on the heaters, then load the patient rack. Hope this gives you a few ideas Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 7. tissue falling off slides (Burnett, Brandy) From: "Burnett, Brandy" Subject: [Histonet] tissue falling off slides Our lab has encountered an issue with tissue falling off of the slides. It is mostly the patient tissue that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated. Thanks Again, Brandy Burnett, HTL --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From darkdaym <@t> comcast.net Fri Aug 15 13:06:52 2014 From: darkdaym <@t> comcast.net (darkdaym@comcast.net) Date: Fri Aug 15 13:07:24 2014 Subject: [Histonet] RE: Glassware Cleaning again In-Reply-To: References: <12ECD7346266D74691EC2BFC75285E452F4B3BDA@BFL323E10.pathmdlabs.local> Message-ID: <53EE4C3C.7070405@comcast.net> Brian, et.al., It's not a typo or and an error. It's one might call a variant spelling. The Merck Index lists Bromcresol Purple, CAS Number 115-40-2. Below is what Sigma lists with the same CAS number. I've noticed the prefix Brom or Bromo on the name of several dyes. Either way, it's the same thing. *114375 * *Sigma-Aldrich * Bromocresol Purple BioReagent, suitable for indicator, Dye content 90 % Synonym: *5,5?-Dibromo-/o/-cresolsulfonphthalein, Bromcresol purple sultone form * On 8/15/2014 11:35 AM, Cooper, Brian wrote: > I noticed the discrepancy in spelling too. I looked online for like 30 minutes, and couldn't find anything called "Bromcresol." Found a lot of vendors selling Bromocresol Purple (and Green for that matter). Best I can figure is that this is a typo on CAP's checklist (that's been there for several years now). > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Friday, August 15, 2014 9:13 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning again > > Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gayle.callis <@t> bresnan.net Fri Aug 15 16:10:27 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Fri Aug 15 16:10:42 2014 Subject: [Histonet] Re: antibody suggestions CD8, FAP, Cd11b, CD11c Message-ID: <000001cfb8cd$583fb3d0$08bf1b70$@bresnan.net> You Wrote: I need your help to find good antibodies for IHC on mouse and human FFPE tissues like normal tissues and tumors. Please share any information you think might be helpful. CD8 for IHC on mouse FFPE spleen or other lymphoid organs. FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors. CD11b for IHC on mouse FFPE spleen or other lymphoid organs.. CD11c for IHC on mouse FFPE spleen or other lymphoid organs. Thanks in advance for all your help. Regards, Mesru ____________________________________________________________________________ ___________________________________________ I can answer some of the murine CD marker questions. CD8 on FFPE mouse tissues will never work. These markers are too strongly cross linked by the aldehyde based fixation, and PFA is not an alternative. You can try HIER and Enzyme digestions until you are blue in the face, and the staining will NOT work. This will also be true of CD11c dendritic cell Clone HL3, and CD11b Mac1. This means you will have to use fresh tissue frozen sections, acetone fixed for any staining success. We use an acetone/absolute ethanol mixture for fixation of murine tissue for all our CD marker staining, especially when you need to do all these CD markers on the same sample. As for positive tissue controls, CD8 Rat antiMouse monoclonal - normal spleen CD11c - Peyers patches from small intestine, lymph node and probably spleen will work. CD11b, Mac1 - we stimulated mouse to produce macrophages in Peyers patches located on small intestine. Stimulation was oral inoculation with an attenuated bacteria. FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors. Go to ABCAM, and look at cross reactivity for this polyclonal antibody. It works on FFPE mouse, human and two other species. I can't provide an answer for FFPE human tissues with any of these CD markers. I strongly suggest you go to BD Bioscience and do a search for the CD markers, especially the mouse that will be either rat and Armenian Hamster monoclonals. Read the Technical data sheets and see what applications will work or not in terms of IHC. We have used BD Bioscience, and eBioscience for our murine CD marker with great success but you have to be aware that CD8 has more than one clone, some working better on solvent fixed murine tissues than others. This is also true of CD11c. ABCAM has an excellent website for these antibodies, but compare prices. There is another fixative that is formalin free, the Becksteads IHC zinc fixative and also PLP i.e. paraformaldehyde-lysine-Periodate. PLP may allow staining of these aldehyde compromised CD markers since the lysine helps get rid of free aldehydes, along with the periodate and is, however, a fixative that is bit tedious to use since it has to be made up fresh every time you use it. Beckstead originally developed IHC zinc fixative in order to do IHC on human tumors, lymphomas if I recall. I have original and other publications for both fixatives showing success with murine CD markers and other species. The IHC zinc fixative allows paraffin embedding and IHC staining of these markers, (also a publication by Nitta et al) Our lab used fresh tissue frozen sections, acetone/alcohol fixed exclusively for all CD marker IHC and/or single, double and triple immunofluroescence protocols. I will be happy to provide acetone/alcohol fixative method and alternative acetone fixations that work with murine CD markers. You cannot use acetone/alcohol for human CD8 or CD4 as the alcohol ruins the antigen - however, FFPE probably will work with human markers. I can also provide publications, information about PLP, Becksteads IHC zinc fixative. PLP recipes can be found on the internet but I have one that is excellent for larger volumes of this fixative. Biocare has information on this fixative with their IHC products too. Gayle M. Callis HTL/HT/MT(ASCP) From ewj <@t> pigsqq.org Sat Aug 16 08:05:06 2014 From: ewj <@t> pigsqq.org (E. Wayne Johnson) Date: Sat Aug 16 08:06:46 2014 Subject: [Histonet] RE: Glassware Cleaning again In-Reply-To: References: <12ECD7346266D74691EC2BFC75285E452F4B3BDA@BFL323E10.pathmdlabs.local> Message-ID: <53EF5702.1080808@pigsqq.org> Bromcresol means Bromocresol. Faint not, but I certainly can't say you won't dye if you leave out the O. The "o" is subject to elision due to its difficulty in pronounciation. Such an occurrence of elision between 2 consonants is called syncope. In English writing an elided vowel is often replaced with an apostrophe to indicate the elision and perhaps demonstrate the dialect of the speaker. Colloquial dialects in writing are too informal for stuffy scientific and medical types, and indeed special meaning is liable to be construed to the presence of a spurious punctuation in the name of a chemical. We don't see {brom'cresol} in the lab {purple, green} but we can hear of an existence where it lives happily under other nomenclature. So "bromcresol" means "bromocresol". And as in American politics, the removal of an "O" might seem to make a difference visually and even might be comfortable to some, but actually would amount absolutely nothing in terms of what is being represented, or the dying that is going on. On 8/16/2014 12:35 AM, Cooper, Brian wrote: > I noticed the discrepancy in spelling too. I looked online for like 30 minutes, and couldn't find anything called "Bromcresol." Found a lot of vendors selling Bromocresol Purple (and Green for that matter). Best I can figure is that this is a typo on CAP's checklist (that's been there for several years now). > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Friday, August 15, 2014 9:13 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning again > > Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > From Susan.Walzer <@t> HCAHealthcare.com Mon Aug 18 01:56:23 2014 From: Susan.Walzer <@t> HCAHealthcare.com (Susan.Walzer@HCAHealthcare.com) Date: Mon Aug 18 01:56:30 2014 Subject: [Histonet] RE: Tissue falling off In-Reply-To: References: <20140815161035.DDF801E8060@trendmess-svr.holyredeemer.local> Message-ID: <4BF03F5404EBDE409AF9232DA74B9DED2FC11CA7E1@FWDCWPMSGCMS09.hca.corpad.net> Tissue that has been properly processed, cut thin and dried well should not be falling off.. We have never needed any additive in our water baths but if we have tough bones or very bloody tissue we use plus slides. PS: we soak tough or bloody tissue in soapy water with some ammonia as it REALLY makes a difference. Susan Walzer HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Friday, August 15, 2014 1:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue falling off Hi - Just a couple of suggestions - I've seen this periodically crop up during the years. There are a couple of things to consider: Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol. Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all. We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off. Your water in the waterbaths is clean - Fresh DI water. Once we had a contaminant in our DI water, and the patient tissues slid right off our slides. We use clean slides, good quality water and super clean waterbaths. Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers. We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle. We now send a rack through first, which turns on the heaters, then load the patient rack. Hope this gives you a few ideas Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 7. tissue falling off slides (Burnett, Brandy) From: "Burnett, Brandy" Subject: [Histonet] tissue falling off slides Our lab has encountered an issue with tissue falling off of the slides. It is mostly the patient tissue that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated. Thanks Again, Brandy Burnett, HTL --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson1 <@t> nhs.net Mon Aug 18 02:23:28 2014 From: kemlo.rogerson1 <@t> nhs.net (Rogerson Kemlo (THE WALTON CENTRE NHS FOUNDATION TRUST - RET)) Date: Mon Aug 18 02:23:51 2014 Subject: [Histonet] Technidata Synergy Histology/ Cytology module Message-ID: <20140818072328.F1AC744840D@nhs-pd1e-esg106.ad1.nhs.net> Dear List, The Walton Centre is one of the few Neuropathology Departments in the UK to use TD Synergy Histo/ Cyto as its LIMS. It is also used in Neurobiochemistry. I am aware that this system is used in the US and wonder if anyone has any experience in its use? On the 'lighter side' I have been a HCPC registered Biomedical Scientist (UK's name for HistoTech) for 44 years as I started as a Junior Technician in March 1970 in Microbiology until I was exiled to Histopathology after a few years. Regards Kemlo Rogerson Pathology Systems Manager The Walton Centre Liverpool ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients NHSmail provides an email address for your career in the NHS and can be accessed anywhere ******************************************************************************************************************** From Reuel.Cornelia <@t> tsrh.org Mon Aug 18 09:27:22 2014 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Mon Aug 18 09:27:27 2014 Subject: [Histonet] CD68 Message-ID: <53F1C6FA.0F7E.00C5.1@tsrh.org> Does anybody out there knows a CD68 antibody that works with pig(Porcine) tissue. We have tried 3 CD68 antibodies already from Santa Cruz, Dako and Novus that they say works with pig tissue but it did not. We tried using heat antigen retrieval and enzyme digestion for paraffin, it did not work. We also use pig frozen tissue and still do not work. Any suggestion. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From GauchV <@t> mail.amc.edu Mon Aug 18 10:06:16 2014 From: GauchV <@t> mail.amc.edu (Gauch, Vicki) Date: Mon Aug 18 10:06:21 2014 Subject: [Histonet] Floating Tissue Question Message-ID: Hi everyone, I have been asked to post this question.... Our lab currently uses Cardinal Plus slides when cutting IHC and ER,PR,etc. on breast tissue. As of late, we are having issues with the tissue floating. It does not appear to be a fixation problem. Does anyone have any recommendations of other plus slide brands or tissue dryers,etc. that have worked well for them ? Please feel free to respond to my e-mail and I will pass the information on. Thank you for any help you can provide us, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From liz <@t> premierlab.com Mon Aug 18 11:08:11 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Aug 18 11:09:02 2014 Subject: [Histonet] CD68 In-Reply-To: <53F1C6FA.0F7E.00C5.1@tsrh.org> References: <53F1C6FA.0F7E.00C5.1@tsrh.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E021B3@SBS2K8.premierlab.local> We use MAC387 not exactly the same as CD68 but stains macrophages, monocytes and I think granulocytes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia [Reuel.Cornelia@tsrh.org] Sent: Monday, August 18, 2014 8:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD68 Does anybody out there knows a CD68 antibody that works with pig(Porcine) tissue. We have tried 3 CD68 antibodies already from Santa Cruz, Dako and Novus that they say works with pig tissue but it did not. We tried using heat antigen retrieval and enzyme digestion for paraffin, it did not work. We also use pig frozen tissue and still do not work. Any suggestion. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From Lacie.Algeo <@t> providence.org Mon Aug 18 11:18:18 2014 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Mon Aug 18 11:18:46 2014 Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 25 In-Reply-To: <59065268-cd48-4b14-bdb4-2aed33a3b6de@BN1AFFO11FD050.protection.gbl> References: <59065268-cd48-4b14-bdb4-2aed33a3b6de@BN1AFFO11FD050.protection.gbl> Message-ID: <24C4B3C167E5694887AB594C7602CE3A0C2E64@WN35104.or.providence.org> I hope I am responding to the correct location :) Brandy....a few questions about your tissue falling off your slides. What kind of tissue? What kind of slides? What kind of stainer? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, August 16, 2014 10:09 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. antibody suggestions CD8, FAP, Cd11b, CD11c (Mesru T) 2. RE: Tissue falling off (Terri Braud) 3. Re: RE: Glassware Cleaning again (darkdaym@comcast.net) 4. Re: antibody suggestions CD8, FAP, Cd11b, CD11c (gayle callis) 5. Re: RE: Glassware Cleaning again (E. Wayne Johnson) ---------------------------------------------------------------------- Message: 1 Date: Fri, 15 Aug 2014 13:24:29 -0400 From: Mesru T Subject: [Histonet] antibody suggestions CD8, FAP, Cd11b, CD11c To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 Dear Histonetters, I need your help to find good antibodies for IHC on mouse and human FFPE tissues like normal tissues and tumors. Please share any information you think might be helpful. CD8 for IHC on mouse FFPE spleen or other lymphoid organs. FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors. CD11b for IHC on mouse FFPE spleen or other lymphoid organs.. CD11c for IHC on mouse FFPE spleen or other lymphoid organs. Thanks in advance for all your help. Regards, Mesru ------------------------------ Message: 2 Date: Fri, 15 Aug 2014 13:52:09 -0400 From: "Terri Braud" Subject: [Histonet] RE: Tissue falling off To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi - Just a couple of suggestions - I've seen this periodically crop up during the years. There are a couple of things to consider: Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol. Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all. We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off. Your water in the waterbaths is clean - Fresh DI water. Once we had a contaminant in our DI water, and the patient tissues slid right off our slides. We use clean slides, good quality water and super clean waterbaths. Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers. We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle. We now send a rack through first, which turns on the heaters, then load the patient rack. Hope this gives you a few ideas Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 7. tissue falling off slides (Burnett, Brandy) From: "Burnett, Brandy" Subject: [Histonet] tissue falling off slides Our lab has encountered an issue with tissue falling off of the slides. It is mostly the patient tissue that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated. Thanks Again, Brandy Burnett, HTL --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ------------------------------ Message: 3 Date: Fri, 15 Aug 2014 13:06:52 -0500 From: darkdaym@comcast.net Subject: Re: [Histonet] RE: Glassware Cleaning again To: histonet@lists.utsouthwestern.edu Message-ID: <53EE4C3C.7070405@comcast.net> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Brian, et.al., It's not a typo or and an error. It's one might call a variant spelling. The Merck Index lists Bromcresol Purple, CAS Number 115-40-2. Below is what Sigma lists with the same CAS number. I've noticed the prefix Brom or Bromo on the name of several dyes. Either way, it's the same thing. *114375 * *Sigma-Aldrich * Bromocresol Purple BioReagent, suitable for indicator, Dye content 90 % Synonym: *5,5?-Dibromo-/o/-cresolsulfonphthalein, Bromcresol purple sultone form * On 8/15/2014 11:35 AM, Cooper, Brian wrote: > I noticed the discrepancy in spelling too. I looked online for like 30 minutes, and couldn't find anything called "Bromcresol." Found a lot of vendors selling Bromocresol Purple (and Green for that matter). Best I can figure is that this is a typo on CAP's checklist (that's been there for several years now). > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Friday, August 15, 2014 9:13 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning again > > Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Fri, 15 Aug 2014 15:10:27 -0600 From: "gayle callis" Subject: [Histonet] Re: antibody suggestions CD8, FAP, Cd11b, CD11c To: Message-ID: <000001cfb8cd$583fb3d0$08bf1b70$@bresnan.net> Content-Type: text/plain; charset="us-ascii" You Wrote: I need your help to find good antibodies for IHC on mouse and human FFPE tissues like normal tissues and tumors. Please share any information you think might be helpful. CD8 for IHC on mouse FFPE spleen or other lymphoid organs. FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors. CD11b for IHC on mouse FFPE spleen or other lymphoid organs.. CD11c for IHC on mouse FFPE spleen or other lymphoid organs. Thanks in advance for all your help. Regards, Mesru ____________________________________________________________________________ ___________________________________________ I can answer some of the murine CD marker questions. CD8 on FFPE mouse tissues will never work. These markers are too strongly cross linked by the aldehyde based fixation, and PFA is not an alternative. You can try HIER and Enzyme digestions until you are blue in the face, and the staining will NOT work. This will also be true of CD11c dendritic cell Clone HL3, and CD11b Mac1. This means you will have to use fresh tissue frozen sections, acetone fixed for any staining success. We use an acetone/absolute ethanol mixture for fixation of murine tissue for all our CD marker staining, especially when you need to do all these CD markers on the same sample. As for positive tissue controls, CD8 Rat antiMouse monoclonal - normal spleen CD11c - Peyers patches from small intestine, lymph node and probably spleen will work. CD11b, Mac1 - we stimulated mouse to produce macrophages in Peyers patches located on small intestine. Stimulation was oral inoculation with an attenuated bacteria. FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors. Go to ABCAM, and look at cross reactivity for this polyclonal antibody. It works on FFPE mouse, human and two other species. I can't provide an answer for FFPE human tissues with any of these CD markers. I strongly suggest you go to BD Bioscience and do a search for the CD markers, especially the mouse that will be either rat and Armenian Hamster monoclonals. Read the Technical data sheets and see what applications will work or not in terms of IHC. We have used BD Bioscience, and eBioscience for our murine CD marker with great success but you have to be aware that CD8 has more than one clone, some working better on solvent fixed murine tissues than others. This is also true of CD11c. ABCAM has an excellent website for these antibodies, but compare prices. There is another fixative that is formalin free, the Becksteads IHC zinc fixative and also PLP i.e. paraformaldehyde-lysine-Periodate. PLP may allow staining of these aldehyde compromised CD markers since the lysine helps get rid of free aldehydes, along with the periodate and is, however, a fixative that is bit tedious to use since it has to be made up fresh every time you use it. Beckstead originally developed IHC zinc fixative in order to do IHC on human tumors, lymphomas if I recall. I have original and other publications for both fixatives showing success with murine CD markers and other species. The IHC zinc fixative allows paraffin embedding and IHC staining of these markers, (also a publication by Nitta et al) Our lab used fresh tissue frozen sections, acetone/alcohol fixed exclusively for all CD marker IHC and/or single, double and triple immunofluroescence protocols. I will be happy to provide acetone/alcohol fixative method and alternative acetone fixations that work with murine CD markers. You cannot use acetone/alcohol for human CD8 or CD4 as the alcohol ruins the antigen - however, FFPE probably will work with human markers. I can also provide publications, information about PLP, Becksteads IHC zinc fixative. PLP recipes can be found on the internet but I have one that is excellent for larger volumes of this fixative. Biocare has information on this fixative with their IHC products too. Gayle M. Callis HTL/HT/MT(ASCP) ------------------------------ Message: 5 Date: Sat, 16 Aug 2014 21:05:06 +0800 From: "E. Wayne Johnson" Subject: Re: [Histonet] RE: Glassware Cleaning again To: "Cooper, Brian" , Laurie Colbert , "Histonet Post (histonet@lists.utsouthwestern.edu)" Message-ID: <53EF5702.1080808@pigsqq.org> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Bromcresol means Bromocresol. Faint not, but I certainly can't say you won't dye if you leave out the O. The "o" is subject to elision due to its difficulty in pronounciation. Such an occurrence of elision between 2 consonants is called syncope. In English writing an elided vowel is often replaced with an apostrophe to indicate the elision and perhaps demonstrate the dialect of the speaker. Colloquial dialects in writing are too informal for stuffy scientific and medical types, and indeed special meaning is liable to be construed to the presence of a spurious punctuation in the name of a chemical. We don't see {brom'cresol} in the lab {purple, green} but we can hear of an existence where it lives happily under other nomenclature. So "bromcresol" means "bromocresol". And as in American politics, the removal of an "O" might seem to make a difference visually and even might be comfortable to some, but actually would amount absolutely nothing in terms of what is being represented, or the dying that is going on. On 8/16/2014 12:35 AM, Cooper, Brian wrote: > I noticed the discrepancy in spelling too. I looked online for like 30 minutes, and couldn't find anything called "Bromcresol." Found a lot of vendors selling Bromocresol Purple (and Green for that matter). Best I can figure is that this is a typo on CAP's checklist (that's been there for several years now). > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Friday, August 15, 2014 9:13 AM > To: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: [Histonet] Glassware Cleaning again > > Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. > > Laurie Colbert, HT (ASCP) > Histology Supervisor > PATH MD > 8158 Beverly Blvd. > Los Angeles, CA 90048 > (323) 648-3214 direct > (424) 245-7284 main lab > > The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 25 ***************************************** ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From jshelley <@t> sanfordburnham.org Mon Aug 18 14:06:17 2014 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Mon Aug 18 14:06:25 2014 Subject: [Histonet] Florida Society of Histotechnology Membership Renewal Message-ID: Hi All, I wanted to inform anyone who may be interested, that we are having open enrollment for FSH membership. It is a $35 charge for a September to August membership cycle. See the link below so you can go to renew or become a new member of the Society. As a member, you will receive: * The FSH newsletter posted on the FSH website. It will keep you updated on regional, state and national events, job opportunities, advertisements and products. * Continuing education opportunities at regional and state meetings at a discounted rate. * Self-study continuing education opportunities through our newsletter offerings * Access to updated profession information on the FSH website at www.fshgroup.org including links to NSH, ASCP, and Clinical Lab Board * Continuing Education Credits provided by FSH free of charge * Representation at the Board of Clinical Laboratory meetings * Representation in the House of Delegates during the National Society for Histotechnology Symposium/Convention * Updates from the NSH Regional Director If you renew by OCTOBER 1, 2014, you will be entered into a drawing. You could be the proud owner of Freida Carson's book: "Histotechnology: A Self Instructional Text (Third Edition)". If you renew online, you will have TWO chances in the drawing!!! Here is the link to become a member: https://www.regonline.com?eventID=1604130&rTypeID=358022 Kind Regards! John J Shelley FSH President 2014-2016 From nicole <@t> dlcjax.com Mon Aug 18 16:03:27 2014 From: nicole <@t> dlcjax.com (Nicole Tatum) Date: Mon Aug 18 16:03:34 2014 Subject: [Histonet] Is there a Law for refusal of pathology services. Message-ID: <1408395838634.44639@dlcjax.com> Please help, We had a patient today who had a punch bx of what is believed to be a clinical dermatofibroma. The patient stated they did not wish for the specimen to be sent for clinical testing. Our ARNP discussed the need for pathology at length and the patient stated she was a nurse and could sign a waiver denying pathology services. I have googled and gooled trying to find any specific law or statue. I can only find information pertaining to research or donated tissue. Stating a person no longer has rights "or ownership"to the tissue once consented and removed, but this case is not for research. Could someone pls share an actual law with me. Seems silly to consent to the surgery but not to the diagnosis. Im not sure what to do at this point. Have them sign a document on our company letterhead stating there denial of services? Hold the tissue hold long? Accession it but only do gross description? Charge anything? Any thoughts or imformation would be greatly appreciated. Nicole Tatum HT BSH From contact <@t> excaliburpathology.com Mon Aug 18 16:16:04 2014 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Aug 18 16:16:08 2014 Subject: [Histonet] Is there a Law for refusal of pathology services. In-Reply-To: <1408395838634.44639@dlcjax.com> References: <1408395838634.44639@dlcjax.com> Message-ID: <1408396564.82661.YahooMailNeo@web162504.mail.bf1.yahoo.com> The tissue sample is proof that the surgery was done.? If the patient does not want a diagnosis, a gross only should be done for medical-legal purposes. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Nicole Tatum To: "histonet@lists.utsouthwestern.edu" Sent: Monday, August 18, 2014 4:03 PM Subject: [Histonet] Is there a Law for refusal of pathology services. Please help, We had a patient today who had a punch bx of what is believed to be a clinical dermatofibroma.? The? patient stated they did not wish for the specimen to be sent for clinical testing. Our ARNP discussed the need for pathology at length and the patient stated she was a nurse and could sign a waiver denying pathology services.? I have googled and gooled trying to find any specific law or statue. I can only find information pertaining to research or donated tissue. Stating a person no longer has rights "or ownership"to the tissue once consented and removed, but this case is not for research.? Could someone pls share an actual law with me. Seems silly to consent to the surgery but not to the diagnosis. Im not sure what to do at this point. Have them sign a document on our company letterhead stating there denial of services? Hold the tissue hold long? Accession it but only do gross description? Charge anything? Any thoughts or imformation would be greatly appreciated. Nicole Tatum HT BSH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Mon Aug 18 17:43:38 2014 From: suetp918 <@t> comcast.net (Sue) Date: Mon Aug 18 17:43:55 2014 Subject: [Histonet] Is there a Law for refusal of pathology services. In-Reply-To: <1408396564.82661.YahooMailNeo@web162504.mail.bf1.yahoo.com> References: <1408395838634.44639@dlcjax.com> <1408396564.82661.YahooMailNeo@web162504.mail.bf1.yahoo.com> Message-ID: <1992885232.376068.1408401818308.JavaMail.root@comcast.net> I agree, if you do not document that a specimen was removed most likely insurance will deny the clain. ? SPaturzo TJU From tony.henwood <@t> health.nsw.gov.au Mon Aug 18 18:52:25 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon Aug 18 18:52:53 2014 Subject: [Histonet] Is there a Law for refusal of pathology services. In-Reply-To: <1408396564.82661.YahooMailNeo@web162504.mail.bf1.yahoo.com> References: <1408395838634.44639@dlcjax.com> <1408396564.82661.YahooMailNeo@web162504.mail.bf1.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00FD460@xmdb04.nch.kids> I agree. Whatever the reason unfortunately, or fortunately, the patient has the right to refuse treatment. Though in this case seems very silly. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Tuesday, 19 August 2014 7:16 AM To: Nicole Tatum; Histonet Subject: Re: [Histonet] Is there a Law for refusal of pathology services. The tissue sample is proof that the surgery was done.? If the patient does not want a diagnosis, a gross only should be done for medical-legal purposes. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: Nicole Tatum To: "histonet@lists.utsouthwestern.edu" Sent: Monday, August 18, 2014 4:03 PM Subject: [Histonet] Is there a Law for refusal of pathology services. Please help, We had a patient today who had a punch bx of what is believed to be a clinical dermatofibroma.? The? patient stated they did not wish for the specimen to be sent for clinical testing. Our ARNP discussed the need for pathology at length and the patient stated she was a nurse and could sign a waiver denying pathology services.? I have googled and gooled trying to find any specific law or statue. I can only find information pertaining to research or donated tissue. Stating a person no longer has rights "or ownership"to the tissue once consented and removed, but this case is not for research.? Could someone pls share an actual law with me. Seems silly to consent to the surgery but not to the diagnosis. Im not sure what to do at this point. Have them sign a document on our company letterhead stating there denial of services? Hold the tissue hold long? Accession it but only do gross description? Charge anything? Any thoughts or imformation would be greatly appreciated. Nicole Tatum HT BSH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From abtdhu <@t> gmail.com Mon Aug 18 20:40:58 2014 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Mon Aug 18 20:41:03 2014 Subject: [Histonet] Tissue falling off In-Reply-To: <53f2314b.e62a3c0a.47f3.ffffa478SMTPIN_ADDED_MISSING@mx.google.com> References: <53f2314b.e62a3c0a.47f3.ffffa478SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <5F5788E6-6919-4E7D-B83B-83E29428A937@gmail.com> Hi Susan, I was so glad that I saw your post regarding tissue falling off. We have had this problem for a while, especially for human bones tissue. This prevents us to do any heat induced antigen retrieval. If it possible to email me how do you mix soapy water with ammonia? How many ml of water? How much soap and ammonia? I am excited to receive your "recipe" and can not wait to test it. Thank you very much. Dorothy Hu MGH endocrine histocore abtdhu@gmail.com > > > Tissue that has been properly processed, cut thin and dried well should not be falling off.. We have never needed any additive in our water baths but if we have tough bones or very bloody tissue we use plus slides. PS: we soak tough or bloody tissue in soapy water with some ammonia as it REALLY makes a difference. > > Susan Walzer HT(ASCP) > From abtdhu <@t> gmail.com Mon Aug 18 20:51:18 2014 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Mon Aug 18 20:51:25 2014 Subject: [Histonet] Vascular contrast agent injected tissue In-Reply-To: <53f2314b.e62a3c0a.47f3.ffffa478SMTPIN_ADDED_MISSING@mx.google.com> References: <53f2314b.e62a3c0a.47f3.ffffa478SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi histoneters, Does any one out there to process tissue in a tissue process machine after lead or barium agents introduced to your tissue? It will do any harm to the machine? Get clog or contaminate other tissue? Thanks for your help. Dorothy Hu MGH endocrine histocore Sent from my iPad > On Aug 18, 2014, at 1:00 PM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Tissue falling off (Susan.Walzer@HCAHealthcare.com) > 2. Technidata Synergy Histology/ Cytology module > (Rogerson Kemlo (THE WALTON CENTRE NHS FOUNDATION TRUST - RET)) > 3. CD68 (Reuel Cornelia) > 4. Floating Tissue Question (Gauch, Vicki) > 5. RE: CD68 (Elizabeth Chlipala) > 6. RE: Histonet Digest, Vol 129, Issue 25 (Algeo, Lacie A) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 18 Aug 2014 01:56:23 -0500 > From: > Subject: [Histonet] RE: Tissue falling off > To: , > Message-ID: > <4BF03F5404EBDE409AF9232DA74B9DED2FC11CA7E1@FWDCWPMSGCMS09.hca.corpad.net> > > Content-Type: text/plain; charset="us-ascii" > > Tissue that has been properly processed, cut thin and dried well should not be falling off.. We have never needed any additive in our water baths but if we have tough bones or very bloody tissue we use plus slides. PS: we soak tough or bloody tissue in soapy water with some ammonia as it REALLY makes a difference. > > Susan Walzer HT(ASCP) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud > Sent: Friday, August 15, 2014 1:52 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Tissue falling off > > Hi - Just a couple of suggestions - > I've seen this periodically crop up during the years. There are a couple of things to consider: > Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol. > Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all. We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off. > Your water in the waterbaths is clean - Fresh DI water. Once we had a contaminant in our DI water, and the patient tissues slid right off our slides. We use clean slides, good quality water and super clean waterbaths. > Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers. We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle. We now send a rack through first, which turns on the heaters, then load the patient rack. > Hope this gives you a few ideas > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > 7. tissue falling off slides (Burnett, Brandy) > From: "Burnett, Brandy" > Subject: [Histonet] tissue falling off slides > > Our lab has encountered an issue with tissue falling off of the slides. > It is mostly the patient tissue > that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated. > Thanks Again, > Brandy Burnett, HTL > > > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Mon, 18 Aug 2014 08:23:28 +0100 > From: "Rogerson Kemlo (THE WALTON CENTRE NHS FOUNDATION TRUST - RET)" > > Subject: [Histonet] Technidata Synergy Histology/ Cytology module > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: <20140818072328.F1AC744840D@nhs-pd1e-esg106.ad1.nhs.net> > Content-Type: text/plain; charset="iso-8859-1" > > Dear List, > > The Walton Centre is one of the few Neuropathology Departments in the UK to use TD Synergy Histo/ Cyto as its LIMS. It is also used in Neurobiochemistry. > > I am aware that this system is used in the US and wonder if anyone has any experience in its use? > > On the 'lighter side' I have been a HCPC registered Biomedical Scientist (UK's name for HistoTech) for 44 years as I started as a Junior Technician in March 1970 in Microbiology until I was exiled to Histopathology after a few years. > > Regards > > Kemlo Rogerson > Pathology Systems Manager > The Walton Centre > Liverpool > > ******************************************************************************************************************** > > This message may contain confidential information. If you are not the intended recipient please inform the > sender that you have received the message in error before deleting it. > Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: > to do so is strictly prohibited and may be unlawful. > > Thank you for your co-operation. > > NHSmail is the secure email and directory service available for all NHS staff in England and Scotland > NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and GSi recipients > NHSmail provides an email address for your career in the NHS and can be accessed anywhere > > ******************************************************************************************************************** > > > ------------------------------ > > Message: 3 > Date: Mon, 18 Aug 2014 09:27:22 -0500 > From: "Reuel Cornelia" > Subject: [Histonet] CD68 > To: > Message-ID: <53F1C6FA.0F7E.00C5.1@tsrh.org> > Content-Type: text/plain; charset="us-ascii" > > Does anybody out there knows a CD68 antibody that works with > pig(Porcine) tissue. We have tried 3 CD68 antibodies already from Santa > Cruz, Dako and Novus that they say works with pig tissue but it did not. > We tried using heat antigen retrieval and enzyme digestion for paraffin, > it did not work. We also use pig frozen tissue and still do not work. > Any suggestion. > > > > > > Reuel Cornelia, BS MT, AMT > Cellular Pathology > Texas Scottish Rite Hospital for Children > 2222 Welborn Street > Dallas, TX 75219 > Tel: 214-559-7766 > fax: 214-559-7768 > > ------------------------------ > > Message: 4 > Date: Mon, 18 Aug 2014 15:06:16 +0000 > From: "Gauch, Vicki" > Subject: [Histonet] Floating Tissue Question > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi everyone, > I have been asked to post this question.... Our lab currently uses Cardinal Plus slides when cutting IHC and ER,PR,etc. on breast tissue. As of late, we are having issues with the tissue floating. It does not appear to be a fixation problem. Does anyone have any recommendations of other plus slide brands or tissue dryers,etc. that have worked well for them ? Please feel free to respond to my e-mail and I will pass the information on. > Thank you for any help you can provide us, > Vicki Gauch > AMCH > Albany, NY > > > ----------------------------------------- > CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. > > > ------------------------------ > > Message: 5 > Date: Mon, 18 Aug 2014 10:08:11 -0600 > From: Elizabeth Chlipala > Subject: RE: [Histonet] CD68 > To: Reuel Cornelia , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <14E2C6176416974295479C64A11CB9AE019C79E021B3@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > We use MAC387 not exactly the same as CD68 but stains macrophages, monocytes and I think granulocytes. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia [Reuel.Cornelia@tsrh.org] > Sent: Monday, August 18, 2014 8:27 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CD68 > > Does anybody out there knows a CD68 antibody that works with > pig(Porcine) tissue. We have tried 3 CD68 antibodies already from Santa > Cruz, Dako and Novus that they say works with pig tissue but it did not. > We tried using heat antigen retrieval and enzyme digestion for paraffin, > it did not work. We also use pig frozen tissue and still do not work. > Any suggestion. > > > > > > Reuel Cornelia, BS MT, AMT > Cellular Pathology > Texas Scottish Rite Hospital for Children > 2222 Welborn Street > Dallas, TX 75219 > Tel: 214-559-7766 > fax: 214-559-7768 > > > ------------------------------ > > Message: 6 > Date: Mon, 18 Aug 2014 16:18:18 +0000 > From: "Algeo, Lacie A" > Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 25 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <24C4B3C167E5694887AB594C7602CE3A0C2E64@WN35104.or.providence.org> > Content-Type: text/plain; charset="us-ascii" > > I hope I am responding to the correct location :) > Brandy....a few questions about your tissue falling off your slides. What kind of tissue? What kind of slides? What kind of stainer? > Thanks, > Lacie > > Lacie Algeo, HTL (ASCP) MBCM > Histology Supervisor > Providence Sacred Heart Medical Center Laboratory > 101 W 8th Avenue > L-2 > Spokane, WA 99204 > 509-474-4418 > FAX 509-474-2052 > lacie.algeo@providence.org > > > This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu > Sent: Saturday, August 16, 2014 10:09 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 129, Issue 25 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. antibody suggestions CD8, FAP, Cd11b, CD11c (Mesru T) > 2. RE: Tissue falling off (Terri Braud) > 3. Re: RE: Glassware Cleaning again (darkdaym@comcast.net) > 4. Re: antibody suggestions CD8, FAP, Cd11b, CD11c (gayle callis) > 5. Re: RE: Glassware Cleaning again (E. Wayne Johnson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 15 Aug 2014 13:24:29 -0400 > From: Mesru T > Subject: [Histonet] antibody suggestions CD8, FAP, Cd11b, CD11c > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Dear Histonetters, > > > I need your help to find good antibodies for IHC on mouse and human FFPE tissues like normal tissues and tumors. Please share any information you think might be helpful. > > CD8 for IHC on mouse FFPE spleen or other lymphoid organs. > FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors. > CD11b for IHC on mouse FFPE spleen or other lymphoid organs.. > CD11c for IHC on mouse FFPE spleen or other lymphoid organs. > > Thanks in advance for all your help. > > Regards, > Mesru > > > ------------------------------ > > Message: 2 > Date: Fri, 15 Aug 2014 13:52:09 -0400 > From: "Terri Braud" > Subject: [Histonet] RE: Tissue falling off > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi - Just a couple of suggestions - > I've seen this periodically crop up during the years. There are a couple of things to consider: > Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol. > Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all. We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off. > Your water in the waterbaths is clean - Fresh DI water. Once we had a contaminant in our DI water, and the patient tissues slid right off our slides. We use clean slides, good quality water and super clean waterbaths. > Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers. We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle. We now send a rack through first, which turns on the heaters, then load the patient rack. > Hope this gives you a few ideas > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > 7. tissue falling off slides (Burnett, Brandy) > From: "Burnett, Brandy" > Subject: [Histonet] tissue falling off slides > > Our lab has encountered an issue with tissue falling off of the slides. > It is mostly the patient tissue > that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated. > Thanks Again, > Brandy Burnett, HTL > > > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > > > ------------------------------ > > Message: 3 > Date: Fri, 15 Aug 2014 13:06:52 -0500 > From: darkdaym@comcast.net > Subject: Re: [Histonet] RE: Glassware Cleaning again > To: histonet@lists.utsouthwestern.edu > Message-ID: <53EE4C3C.7070405@comcast.net> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Brian, et.al., > It's not a typo or and an error. It's one might call a variant spelling. The Merck Index lists Bromcresol Purple, CAS Number 115-40-2. > Below is what Sigma lists with the same CAS number. I've noticed the prefix Brom or Bromo on the name of several dyes. Either way, it's the same thing. > > *114375 * *Sigma-Aldrich * > > > Bromocresol Purple > > > BioReagent, suitable for indicator, Dye content 90 % > > Synonym: *5,5?-Dibromo-/o/-cresolsulfonphthalein, Bromcresol purple sultone form > > > * >> On 8/15/2014 11:35 AM, Cooper, Brian wrote: >> I noticed the discrepancy in spelling too. I looked online for like 30 minutes, and couldn't find anything called "Bromcresol." Found a lot of vendors selling Bromocresol Purple (and Green for that matter). Best I can figure is that this is a typo on CAP's checklist (that's been there for several years now). >> >> Thanks, >> >> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor >> Department of Pathology and Laboratory Medicine >> Children's Hospital Los Angeles >> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 >> bcooper@chla.usc.edu >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert >> Sent: Friday, August 15, 2014 9:13 AM >> To: Histonet Post (histonet@lists.utsouthwestern.edu) >> Subject: [Histonet] Glassware Cleaning again >> >> Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. >> >> Laurie Colbert, HT (ASCP) >> Histology Supervisor >> PATH MD >> 8158 Beverly Blvd. >> Los Angeles, CA 90048 >> (323) 648-3214 direct >> (424) 245-7284 main lab >> >> The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> --------------------------------------------------------------------- >> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, >> is for the sole use of the intended recipient(s) and may contain confidential >> or legally privileged information. Any unauthorized review, use, disclosure >> or distribution is prohibited. If you are not the intended recipient, please >> contact the sender by reply e-mail and destroy all copies of this original message. >> >> --------------------------------------------------------------------- >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Fri, 15 Aug 2014 15:10:27 -0600 > From: "gayle callis" > Subject: [Histonet] Re: antibody suggestions CD8, FAP, Cd11b, CD11c > To: > Message-ID: <000001cfb8cd$583fb3d0$08bf1b70$@bresnan.net> > Content-Type: text/plain; charset="us-ascii" > > You Wrote: > > > > I need your help to find good antibodies for IHC on mouse and human FFPE > > tissues like normal tissues and tumors. Please share any information you > > think might be helpful. > > > > CD8 for IHC on mouse FFPE spleen or other lymphoid organs. > > FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors. > > CD11b for IHC on mouse FFPE spleen or other lymphoid organs.. > > CD11c for IHC on mouse FFPE spleen or other lymphoid organs. > > > > Thanks in advance for all your help. > > > > Regards, > > Mesru > > ____________________________________________________________________________ > ___________________________________________ > > > > I can answer some of the murine CD marker questions. > > > > CD8 on FFPE mouse tissues will never work. These markers are too strongly > cross linked by the aldehyde based fixation, and PFA is not an alternative. > You can try HIER and Enzyme digestions until you are blue in the face, and > the staining will NOT work. This will also be true of CD11c dendritic cell > Clone HL3, and CD11b Mac1. > > > > This means you will have to use fresh tissue frozen sections, acetone fixed > for any staining success. We use an acetone/absolute ethanol mixture for > fixation of murine tissue for all our CD marker staining, especially when > you need to do all these CD markers on the same sample. > > > > As for positive tissue controls, > > > > CD8 Rat antiMouse monoclonal - normal spleen > > CD11c - Peyers patches from small intestine, lymph node and probably spleen > will work. > > CD11b, Mac1 - we stimulated mouse to produce macrophages in Peyers patches > located on small intestine. Stimulation was oral inoculation with an > attenuated bacteria. > > FAP (Fibroblast Activated protein) for IHC on mouse and human FFPE tumors. > Go to ABCAM, and look at cross reactivity for this polyclonal antibody. It > works on FFPE mouse, human and two other species. > > > > > > I can't provide an answer for FFPE human tissues with any of these CD > markers. > > > > I strongly suggest you go to BD Bioscience and do a search for the CD > markers, especially the mouse that will be either rat and Armenian Hamster > monoclonals. Read the Technical data sheets and see what applications will > work or not in terms of IHC. We have used BD Bioscience, and eBioscience for > our murine CD marker with great success but you have to be aware that CD8 > has more than one clone, some working better on solvent fixed murine tissues > than others. This is also true of CD11c. ABCAM has an excellent website > for these antibodies, but compare prices. > > > > There is another fixative that is formalin free, the Becksteads IHC zinc > fixative and also PLP i.e. paraformaldehyde-lysine-Periodate. PLP may allow > staining of these aldehyde compromised CD markers since the lysine helps get > rid of free aldehydes, along with the periodate and is, however, a fixative > that is bit tedious to use since it has to be made up fresh every time you > use it. Beckstead originally developed IHC zinc fixative in order to do IHC > on human tumors, lymphomas if I recall. I have original and other > publications for both fixatives showing success with murine CD markers and > other species. The IHC zinc fixative allows paraffin embedding and IHC > staining of these markers, (also a publication by Nitta et al) > > > > Our lab used fresh tissue frozen sections, acetone/alcohol fixed exclusively > for all CD marker IHC and/or single, double and triple immunofluroescence > protocols. > > > > I will be happy to provide acetone/alcohol fixative method and alternative > acetone fixations that work with murine CD markers. You cannot use > acetone/alcohol for human CD8 or CD4 as the alcohol ruins the antigen - > however, FFPE probably will work with human markers. I can also provide > publications, information about PLP, Becksteads IHC zinc fixative. PLP > recipes can be found on the internet but I have one that is excellent for > larger volumes of this fixative. Biocare has information on this fixative > with their IHC products too. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > ------------------------------ > > Message: 5 > Date: Sat, 16 Aug 2014 21:05:06 +0800 > From: "E. Wayne Johnson" > Subject: Re: [Histonet] RE: Glassware Cleaning again > To: "Cooper, Brian" , Laurie Colbert > , "Histonet Post > (histonet@lists.utsouthwestern.edu)" > > Message-ID: <53EF5702.1080808@pigsqq.org> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Bromcresol means Bromocresol. > > Faint not, but I certainly can't say you won't dye if you leave out the O. > > The "o" is subject to elision due to its difficulty in pronounciation. > > Such an occurrence of elision between 2 consonants is called syncope. > > In English writing an elided vowel is often replaced with an apostrophe to > indicate the elision and perhaps demonstrate the dialect of the speaker. > > Colloquial dialects in writing are too informal for stuffy scientific > and medical types, and indeed > special meaning is liable to be construed to the presence of a spurious > punctuation in the name of a chemical. > > We don't see {brom'cresol} in > the lab {purple, green} but we can hear of an existence > where it lives happily under other nomenclature. > > So "bromcresol" means "bromocresol". > > And as in American politics, > the removal of an "O" might seem to make a difference visually > and even might be comfortable to some, > but actually would amount absolutely nothing in terms of what is > being represented, or the dying that is going on. > > > >> On 8/16/2014 12:35 AM, Cooper, Brian wrote: >> I noticed the discrepancy in spelling too. I looked online for like 30 minutes, and couldn't find anything called "Bromcresol." Found a lot of vendors selling Bromocresol Purple (and Green for that matter). Best I can figure is that this is a typo on CAP's checklist (that's been there for several years now). >> >> Thanks, >> >> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor >> Department of Pathology and Laboratory Medicine >> Children's Hospital Los Angeles >> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 >> bcooper@chla.usc.edu >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert >> Sent: Friday, August 15, 2014 9:13 AM >> To: Histonet Post (histonet@lists.utsouthwestern.edu) >> Subject: [Histonet] Glassware Cleaning again >> >> Is bromocresol purple the same as bromcresol purple? The CAP question regarding glassware cleaning refers to bromcresol purple, but I ordered a powder and it is labeled as bromocresol purple. >> >> Laurie Colbert, HT (ASCP) >> Histology Supervisor >> PATH MD >> 8158 Beverly Blvd. >> Los Angeles, CA 90048 >> (323) 648-3214 direct >> (424) 245-7284 main lab >> >> The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> --------------------------------------------------------------------- >> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, >> is for the sole use of the intended recipient(s) and may contain confidential >> or legally privileged information. Any unauthorized review, use, disclosure >> or distribution is prohibited. If you are not the intended recipient, please >> contact the sender by reply e-mail and destroy all copies of this original message. >> >> --------------------------------------------------------------------- > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 129, Issue 25 > ***************************************** > > ________________________________ > > This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 129, Issue 26 > ***************************************** From talulahgosh <@t> gmail.com Tue Aug 19 06:00:19 2014 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Tue Aug 19 06:00:26 2014 Subject: [Histonet] Is there a Law for refusal of pathology services. In-Reply-To: <1408395838634.44639@dlcjax.com> References: <1408395838634.44639@dlcjax.com> Message-ID: What is the reasoning for this?? I think the insurance company would want a diagnosis! Does this person enjoy surgery for the fun of it and not actually need it? Or maybe she has another problem she doesn't want her insurance to know about? Although if she has insurance she can't be retroactively declined, can she? How odd. Emily On Aug 18, 2014 5:04 PM, "Nicole Tatum" wrote: > Please help, > > > > We had a patient today who had a punch bx of what is believed to be a > clinical dermatofibroma. The patient stated they did not wish for the > specimen to be sent for clinical testing. Our ARNP discussed the need for > pathology at length and the patient stated she was a nurse and could sign a > waiver denying pathology services. I have googled and gooled trying to > find any specific law or statue. I can only find information pertaining to > research or donated tissue. Stating a person no longer has rights "or > ownership"to the tissue once consented and removed, but this case is not > for research. Could someone pls share an actual law with me. Seems silly > to consent to the surgery but not to the diagnosis. Im not sure what to do > at this point. Have them sign a document on our company letterhead stating > there denial of services? Hold the tissue hold long? Accession it but only > do gross description? Charge anything? Any thoughts or imformation would be > greatly appreciated. > > > > Nicole Tatum HT BSH > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mturner <@t> CSILaboratories.com Tue Aug 19 07:27:23 2014 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Tue Aug 19 07:27:44 2014 Subject: [Histonet] RE: Is there a Law for refusal of pathology services. In-Reply-To: <1408395838634.44639@dlcjax.com> References: <1408395838634.44639@dlcjax.com> Message-ID: <643626B74DE2814D8537057F40E1A10B0CB063A4@CSI-MX-NODEA.CSI-LABS.local> Nicole, Does the surgical consent form address the issue, including consent for further testing? Many do, and this should cover the issue. She does, however, have the right to revoke any prior permissions at any time she decides to change her mind. I would have her sign a statement that she is refusing to allow diagnosis and include it as part of a gross-only report. Holding the tissue as documentation of the procedure is wise, and can be justified to the patient rather easily since giving the specimen back to the client involves handing them a hazardous substance (formalin), something the OSHA folks frown upon. I'm also curious how the payors will view this. Sounds like a ready-made excuse for instance denial. Keep us posted. Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum Sent: Monday, August 18, 2014 5:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Is there a Law for refusal of pathology services. Please help, We had a patient today who had a punch bx of what is believed to be a clinical dermatofibroma. The patient stated they did not wish for the specimen to be sent for clinical testing. Our ARNP discussed the need for pathology at length and the patient stated she was a nurse and could sign a waiver denying pathology services. I have googled and gooled trying to find any specific law or statue. I can only find information pertaining to research or donated tissue. Stating a person no longer has rights "or ownership"to the tissue once consented and removed, but this case is not for research. Could someone pls share an actual law with me. Seems silly to consent to the surgery but not to the diagnosis. Im not sure what to do at this point. Have them sign a document on our company letterhead stating there denial of services? Hold the tissue hold long? Accession it but only do gross description? Charge anything? Any thoughts or imformation would be greatly appreciated. Nicole Tatum HT BSH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides <@t> eae.csic.es Tue Aug 19 08:13:18 2014 From: j.benavides <@t> eae.csic.es (Julio Benavides) Date: Tue Aug 19 08:16:40 2014 Subject: [Histonet] isopropanol for tissue processing Message-ID: <53F34D6E.9030501@eae.csic.es> Hi, In our histology lab, we are considering to move from ethanol to isopropanol for processing formalin fixed samples (post mortem samples, so big samples). I have been diving in the histonet archives and have found that the subject has been laready discussed, but somehow I cannot find a definitive message stating "Yes, we have tried and it works perfectly. This is our protocol". So here I am, writing the forum to see if anyone can kindly confirm that the move from ethanol to isopropanol is not a traumatic one, and if any of you has eliminated the xylene as clearing agent as well (and what have you used instead. Isopropanol/paraffin at 50c or straight into paraffin?) I have been speaking with Rene J Buesa who, besides a very helpful person, is highly enthusiast in the use of isopropanol. He has almost converted me to the isopropanol side! Thank you very much for your time and help Regards Julio Benavides Instituto de Ganader?a de Monta?a (CSIC-University of Leon) Spain From rmire <@t> cvpath.org Tue Aug 19 08:43:08 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Tue Aug 19 08:43:36 2014 Subject: [Histonet] Exakt benchmarks Message-ID: <000601cfbbb3$8daf26c0$a90d7440$@org> Hello Netters, I am seeking information on Exakt technology benchmarks. Those of you using this technology, will you kindly share with me how many specimens/slides can be completed in a work day? I am new to this technology and have been tasked with establishing benchmarks/goals for the lab techs. Thanks Ronda Mire Laboratory Manager/Chief Technologist CVPath Institute 19 Firstfield Rd Gaithersburg, MD 20878 301-208-3570 x112 rmire@cvpath.org From barbara.tibbs <@t> accuratediagnosticlabs.com Tue Aug 19 08:51:32 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Tue Aug 19 08:51:44 2014 Subject: [Histonet] RE: Is there a Law for refusal of pathology services. In-Reply-To: <1992885232.376068.1408401818308.JavaMail.root@comcast.net> References: <1408395838634.44639@dlcjax.com> <1408396564.82661.YahooMailNeo@web162504.mail.bf1.yahoo.com>, <1992885232.376068.1408401818308.JavaMail.root@comcast.net> Message-ID: <1408456270416.63896@accuratediagnosticlabs.com> Also, legally speaking, if it isn't documented, it didn't happen in the eyes of the law. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sue Sent: Monday, August 18, 2014 9:43 PM To: Paula Pierce Cc: Histonet Subject: Re: [Histonet] Is there a Law for refusal of pathology services. I agree, if you do not document that a specimen was removed most likely insurance will deny the clain. SPaturzo TJU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Tue Aug 19 09:34:20 2014 From: twheelock <@t> mclean.harvard.edu (Wheelock, Timothy R.) Date: Tue Aug 19 09:34:38 2014 Subject: [Histonet] isopropanol for tissue processing In-Reply-To: <53F34D6E.9030501@eae.csic.es> References: <53F34D6E.9030501@eae.csic.es> Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9772A3BA7@PHSX10MB11.partners.org> Hi Julio: I have been using isopropanol for as long as I can remember. I work only with post-mortem brain tissue (in standard-sized cassettes) in a neuropathology laboratory at a brain tissue bank. Because the brains have been previously fixed in 10% neutral buffered formalin for a month, I do not have to use formalin on the processor. Anyway, I start out with 30% isopropanol, then 50%, 80%, 95%, 3 stations of 100%, 3 stations of xylene, then into paraffin. I cannot remember whether we used isopropanol in surgical histology years ago, when I was at another hospital, but I think we did. Good luck, Tim Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA, USA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Julio Benavides Sent: Tuesday, August 19, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] isopropanol for tissue processing Hi, In our histology lab, we are considering to move from ethanol to isopropanol for processing formalin fixed samples (post mortem samples, so big samples). I have been diving in the histonet archives and have found that the subject has been laready discussed, but somehow I cannot find a definitive message stating "Yes, we have tried and it works perfectly. This is our protocol". So here I am, writing the forum to see if anyone can kindly confirm that the move from ethanol to isopropanol is not a traumatic one, and if any of you has eliminated the xylene as clearing agent as well (and what have you used instead. Isopropanol/paraffin at 50c or straight into paraffin?) I have been speaking with Rene J Buesa who, besides a very helpful person, is highly enthusiast in the use of isopropanol. He has almost converted me to the isopropanol side! Thank you very much for your time and help Regards Julio Benavides Instituto de Ganader?a de Monta?a (CSIC-University of Leon) Spain _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From billodonnell <@t> catholichealth.net Tue Aug 19 09:45:08 2014 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Tue Aug 19 09:52:29 2014 Subject: [Histonet] RE: Is there a Law for refusal of pathology services. In-Reply-To: <1408456270416.63896@accuratediagnosticlabs.com> References: <1408395838634.44639@dlcjax.com> <1408396564.82661.YahooMailNeo@web162504.mail.bf1.yahoo.com>, <1992885232.376068.1408401818308.JavaMail.root@comcast.net> <1408456270416.63896@accuratediagnosticlabs.com> Message-ID: I believe the question was asking for "laws". A law is different than what insurance companies may or may not prefer. They have 'rules' but have to follow 'laws'. Laws that might concern this are also enacted at both the national and state levels. Any state might differ over another. All I can offer beyond that is, if really concerned, and it sounds like you are, take up the question with your Risk Management folks, or whatever department deals with that kind of stuff. This case is one you are aware of. It might shock you to learn how many times patients do not have their tissues examined, especially in clinics. The best record might the op-notes, as far as the insurance companies are concerned. To assume the patient won't be covered, that she has anterior motive, or that the lab is the sole documentation of a procedure is to make legal assumptions and, frankly, that's not a good idea. All of that said - it is, in my mind, prudent to get a diagnosis, but prudence is different than necessity and patient rights are pretty broad and are rarely narrowly interpreted. Thanks for reading this far down - Bill (Not A Lawyer) O'Donnell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Tuesday, August 19, 2014 8:52 AM To: Sue; Paula Pierce Cc: Histonet Subject: [Histonet] RE: Is there a Law for refusal of pathology services. Also, legally speaking, if it isn't documented, it didn't happen in the eyes of the law. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sue Sent: Monday, August 18, 2014 9:43 PM To: Paula Pierce Cc: Histonet Subject: Re: [Histonet] Is there a Law for refusal of pathology services. I agree, if you do not document that a specimen was removed most likely insurance will deny the clain. SPaturzo TJU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=PW5V5XCh49YsMBD3%2BnZElnBACR0YMDg0K9qQPxv94bc%3D%0A&s=15fc4e176087c51f6d54c59f9058835ce7bc2a997e53de623791cde774d875a3 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=PW5V5XCh49YsMBD3%2BnZElnBACR0YMDg0K9qQPxv94bc%3D%0A&s=15fc4e176087c51f6d54c59f9058835ce7bc2a997e53de623791cde774d875a3 This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From bburnett <@t> CapeCodHealth.org Tue Aug 19 09:54:13 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Tue Aug 19 09:54:23 2014 Subject: [Histonet] RE: Tissue falling off In-Reply-To: References: <20140815161035.DDF801E8060@trendmess-svr.holyredeemer.local>, Message-ID: Teri~ Thanks so much for the very useful feedback... We have only encountered this problem recently and these suggestions will be very useful! ~Brandy ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Terri Braud [tbraud@holyredeemer.com] Sent: Friday, August 15, 2014 1:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Tissue falling off Hi - Just a couple of suggestions - I've seen this periodically crop up during the years. There are a couple of things to consider: Water baths are completely clean - after dumpling water and removing paraffin debris with whatever you use - wipe out the bath with clean absolute alcohol. Be careful where you spray - make sure that if you use anything like Paraguard, or similar stuff that keeps paraffin from sticking, that it is used sparingly, or preferably, not at all. We had a situation where we had a trial sample that someone was using to clean her microtome and forceps and the stuff got everywhere and tissues started falling off. Your water in the waterbaths is clean - Fresh DI water. Once we had a contaminant in our DI water, and the patient tissues slid right off our slides. We use clean slides, good quality water and super clean waterbaths. Is your heater up and running? - Our slides are stained on a stainer with built in slide dryers. We used to have a problem with the first rack of patient slides' tissue falling off because the slide dryer was not hot enough for our short dry cycle. We now send a rack through first, which turns on the heaters, then load the patient rack. Hope this gives you a few ideas Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 7. tissue falling off slides (Burnett, Brandy) From: "Burnett, Brandy" Subject: [Histonet] tissue falling off slides Our lab has encountered an issue with tissue falling off of the slides. It is mostly the patient tissue that is falling off. Some of the Techs are using control slides that have been pre-cut and I am wondering if this might be part of the problem. Any information would be very much appreciated. Thanks Again, Brandy Burnett, HTL --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From Lisa.White3 <@t> va.gov Tue Aug 19 10:06:35 2014 From: Lisa.White3 <@t> va.gov (White, Lisa M.) Date: Tue Aug 19 10:08:10 2014 Subject: [Histonet] Is there a Law for refusal of pathology services. Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760C4781CE@VHAV09MSGA3.v09.med.va.gov> I would think the patient would have to sign an Against Medical Advise release just as if they were leaving the ER even though you know they were having a heart attack. Should the reason for refusal to have the specimen processed is they do not have insurance and you do a gross only then they will still be charged for a gross only specimen. Lisa White, HT(ASCP) Classification: Internal and External Use\\Not VA Sensitive This message has been categorized by White, Lisa M. on Tuesday, August 19, 2014 at 11:06:35 AM in accordance with VA Handbook 6500 From BZIMMERM <@t> gru.edu Tue Aug 19 10:16:20 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Aug 19 10:16:31 2014 Subject: [Histonet] Georgia Society for Histotechnology Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF870EB9@EX-MLB-03.ad.georgiahealth.edu> The Georgia Society for Histotechnology is planning for next year's HISTOPALOOZA! HISTOPALOOZA APRIL 17 - 19, 2015 LEGACY LODGE AT LAKE LANIER ISLANDS This is a call for abstracts for workshop speakers. We will once again offer the HT/HTL review, the QIHC review, and the microscopic anatomy by Ely Klar. The GSH offers free membership and we encourage you to join NSH with is $$ savings. Take a look at our website and contact any BOD member for information about what the GSH can offer you. Our next newsletter will be on our website by September 6th, with updates on HISTOPALOOZA! 2015 Here's the link for that awesome place at Lake Lanier. It looks like a ski resort and it's hard to believe you're in Atlanta, to be honest. http://www.lakelanierislands.com/meetings?rt=google|cpc|LAL03-Lanier-Islands-Meetings-Brand|legacy%20lodge%20and%20conference%20center&gclid=CKvy7IfOn8ACFc1i7AodfFcARQ Hope I see many of you in Austin! Warm Regards, Wanda K Simons HT, ASCP GSH President ws/bz From tpodawiltz <@t> lrgh.org Tue Aug 19 10:20:53 2014 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Aug 19 10:21:03 2014 Subject: [Histonet] RE: Is there a Law for refusal of pathology services. In-Reply-To: References: <1408395838634.44639@dlcjax.com> <1408396564.82661.YahooMailNeo@web162504.mail.bf1.yahoo.com>, <1992885232.376068.1408401818308.JavaMail.root@comcast.net> <1408456270416.63896@accuratediagnosticlabs.com> Message-ID: <38667E7FB77ECD4E91BFAEB8D9863863261589EA6B@LRGHEXVS1.practice.lrgh.org> I would start with CAP/CLIA for their guidelines. Then check to see what the state regulations would be. Tom Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, August 19, 2014 10:45 AM To: Histonet Subject: [Histonet] RE: Is there a Law for refusal of pathology services. I believe the question was asking for "laws". A law is different than what insurance companies may or may not prefer. They have 'rules' but have to follow 'laws'. Laws that might concern this are also enacted at both the national and state levels. Any state might differ over another. All I can offer beyond that is, if really concerned, and it sounds like you are, take up the question with your Risk Management folks, or whatever department deals with that kind of stuff. This case is one you are aware of. It might shock you to learn how many times patients do not have their tissues examined, especially in clinics. The best record might the op-notes, as far as the insurance companies are concerned. To assume the patient won't be covered, that she has anterior motive, or that the lab is the sole documentation of a procedure is to make legal assumptions and, frankly, that's not a good idea. All of that said - it is, in my mind, prudent to get a diagnosis, but prudence is different than necessity and patient rights are pretty broad and are rarely narrowly interpreted. Thanks for reading this far down - Bill (Not A Lawyer) O'Donnell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Tuesday, August 19, 2014 8:52 AM To: Sue; Paula Pierce Cc: Histonet Subject: [Histonet] RE: Is there a Law for refusal of pathology services. Also, legally speaking, if it isn't documented, it didn't happen in the eyes of the law. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sue Sent: Monday, August 18, 2014 9:43 PM To: Paula Pierce Cc: Histonet Subject: Re: [Histonet] Is there a Law for refusal of pathology services. I agree, if you do not document that a specimen was removed most likely insurance will deny the clain. SPaturzo TJU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=PW5V5XCh49YsMBD3%2BnZElnBACR0YMDg0K9qQPxv94bc%3D%0A&s=15fc4e176087c51f6d54c59f9058835ce7bc2a997e53de623791cde774d875a3 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=PW5V5XCh49YsMBD3%2BnZElnBACR0YMDg0K9qQPxv94bc%3D%0A&s=15fc4e176087c51f6d54c59f9058835ce7bc2a997e53de623791cde774d875a3 This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Charles.Stewart <@t> leicabiosystems.com Tue Aug 19 10:24:14 2014 From: Charles.Stewart <@t> leicabiosystems.com (Stewart, Charles) Date: Tue Aug 19 10:24:56 2014 Subject: [Histonet] n Message-ID: <617A6155-9EE0-43A0-AD0E-BD5A445D74D5@leicabiosystems.com> Respectfully, Charles Stewart Field Support Specialist Leica Biosystems 412-737-7380 charles.stewart@leicabiosystems.com Please be advised that this email may contain confidential information. If you are not the intended recipient, please notify us by email by replying to the sender and delete this message. The sender disclaims that the content of this email constitutes an offer to enter into, or the acceptance of, any agreement; provided that the foregoing does not invalidate the binding effect of any digital or other electronic reproduction of a manual signature that is included in any attachment. From modz9636 <@t> gmail.com Tue Aug 19 11:45:40 2014 From: modz9636 <@t> gmail.com (M.O.) Date: Tue Aug 19 11:45:43 2014 Subject: [Histonet] bone saw for cutting slabs Message-ID: Histoland! Happy Tuesday! I just wanted to get your feedback on cutting slabs from human femora for histopathological analysis. At them moment we are just using a hack saw to cut 7mm slabs from femora. We notice some marks on the cartilage from sawing, so when we cut the tissue down after decalcification for histological preparation, we cut the thickness down to 4mm and remove the "damaged" tissue. Would using some sort of bone saw damage the tissue even more or would it be comparable to using a hack saw? Is there a saw that you recommend that is precise and easy to handle that doesn't damage tissue greatly? Thank you so much for your help! Sincerely, Merissa From bburnett <@t> CapeCodHealth.org Tue Aug 19 12:09:45 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Tue Aug 19 12:10:12 2014 Subject: [Histonet] tissue falling off slides Message-ID: I forgot to mention that we are using a 3 in one HIER for target retrieval, instead of running down (xylene-ETOH). Could this also contribute to tissue falling off? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From barbara.tibbs <@t> accuratediagnosticlabs.com Tue Aug 19 12:21:13 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Tue Aug 19 12:21:17 2014 Subject: [Histonet] bone saw for cutting slabs In-Reply-To: References: Message-ID: <1408468851531.12893@accuratediagnosticlabs.com> Most hospitals that I've worked at that needed to cut bone used a Stryker bone saw. The pathologists never mentioned damaged cartilage. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of M.O. Sent: Tuesday, August 19, 2014 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone saw for cutting slabs Histoland! Happy Tuesday! I just wanted to get your feedback on cutting slabs from human femora for histopathological analysis. At them moment we are just using a hack saw to cut 7mm slabs from femora. We notice some marks on the cartilage from sawing, so when we cut the tissue down after decalcification for histological preparation, we cut the thickness down to 4mm and remove the "damaged" tissue. Would using some sort of bone saw damage the tissue even more or would it be comparable to using a hack saw? Is there a saw that you recommend that is precise and easy to handle that doesn't damage tissue greatly? Thank you so much for your help! Sincerely, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smcbride <@t> andrew.cmu.edu Tue Aug 19 12:49:10 2014 From: smcbride <@t> andrew.cmu.edu (Sean McBride) Date: Tue Aug 19 12:49:17 2014 Subject: [Histonet] bone saw for cutting slabs In-Reply-To: References: Message-ID: Hi Merissa, Exakt technologies makes a wonderful saw designed specifically for exactly what you are trying to do with a hack saw. It is a bit pricy though. Contact Linda Durbin at 405-848-5800 for a quote. Alternatively, you can use a wet saw designed for cutting stained glass. Check out the one by Gryphon: Not too expensive & cuts bone well if you take your time. Other vendors like Mar-Med make alternative blades for this saw as well. Good luck, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 571-989-BONE (m) 412-268-8275 (fax) smcbride@andrew.cmu.edu On Aug 19, 2014, at 12:45 PM, M.O. wrote: > Histoland! Happy Tuesday! > > I just wanted to get your feedback on cutting slabs from human femora > for histopathological analysis. > > At them moment we are just using a hack saw to cut 7mm slabs from > femora. We notice some marks on the cartilage from sawing, so when we cut > the tissue down after decalcification for histological preparation, we cut > the thickness down to 4mm and remove the "damaged" tissue. > > Would using some sort of bone saw damage the tissue even more or would it > be comparable to using a hack saw? Is there a saw that you recommend that > is precise and easy to handle that doesn't damage tissue greatly? > > Thank you so much for your help! > > Sincerely, > Merissa > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis <@t> seattlechildrens.org Tue Aug 19 14:07:04 2014 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Tue Aug 19 14:07:09 2014 Subject: [Histonet] OCT Tissue fall out and reembedding Message-ID: <3903BE18914F4440834F0E620415FFCA3CB23151@PPWEXD01d.childrens.sea.kids> Hi everyone. If you have several OCT blocks in which the Tissue has fallen out and to want to salvage them for IHC. What is the best way to do so to minimize freeze-thaw damage. These samples were harvested and then were probably snap frozen in Isopentane surrounded by dry ice and stored at -80 when they were made. The people using these blocks now have had several of the blocks in which the tissue has popped out of the block. We are going to get these blocks for IHC. What is the best way to salvage these tissues so that I can do IHC on them. Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From wbenton <@t> cua.md Tue Aug 19 14:15:27 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Aug 19 14:15:32 2014 Subject: [Histonet] IHC on free floating slices of brain Message-ID: <0B8979A204680A42B93A52B486088CD93938636B14@CUAEXH1.GCU-MD.local> Does anyone have information they could share on performing IHC on whole mount, sledge microtome free floating slices of brain? A Neuropathologist colleague is interested in doing this. Thanks in advance for your help. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Mark.Elliott <@t> hli.ubc.ca Tue Aug 19 16:47:37 2014 From: Mark.Elliott <@t> hli.ubc.ca (Mark Elliott) Date: Tue Aug 19 16:49:49 2014 Subject: [Histonet] whole mount immunofluorescence staining for mouse aorta Message-ID: <53F36389020000D600073D2A@mail.hli.ubc.ca> A colleague of mine has asked if I have any experience wholemount immunofluorescence staining for mouse aorta. I don't have any experience with this so thought I would ask the group for any suggestions/protocols/ tips etc. Thanks Mark From liz <@t> premierlab.com Tue Aug 19 17:09:22 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Aug 19 17:09:27 2014 Subject: [Histonet] whole mount immunofluorescence staining for mouse aorta In-Reply-To: <53F36389020000D600073D2A@mail.hli.ubc.ca> References: <53F36389020000D600073D2A@mail.hli.ubc.ca> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD5F3@SBS2K8.premierlab.local> Mark Not sure why they would want to do this for IF staining. The only thing I can think of that is similar to this is in mouse models of atherosclerosis we have run "en Face" analysis of the mouse aorta. The entire mouse aorta is dissected out, trimmed of fat and cut open via a specific procedure, the entire aorta is then stained with Sudan IV to demonstrate the plaques and then pinned on a tar plate, images are captured for image analysis. It is also common in the studies to run aortic root analysis - we have done this on frozen sections with brightfield IHC. We have experience in all of these aspects of tissue collection and preparation, some of the techniques may apply to what they are trying to do. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Elliott Sent: Tuesday, August 19, 2014 3:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] whole mount immunofluorescence staining for mouse aorta A colleague of mine has asked if I have any experience wholemount immunofluorescence staining for mouse aorta. I don't have any experience with this so thought I would ask the group for any suggestions/protocols/ tips etc. Thanks Mark From j.rowaihi <@t> alborglaboratories.com Wed Aug 20 05:49:18 2014 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Wed Aug 20 05:46:44 2014 Subject: [Histonet] Coverslipper Message-ID: <01f201cfbc64$65f61010$31e23030$@rowaihi@alborglaboratories.com> Dear colleagues Do any one have experience or information about Leica Coverslipper ST5010-CV5030 Workstation stainer. I am planning to add a new autostainer with coverslipper but I am confused between this brand and Tissue-Tek R Prisma R & Coverslipper. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From barbara.tibbs <@t> accuratediagnosticlabs.com Wed Aug 20 08:06:54 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Wed Aug 20 08:07:04 2014 Subject: [Histonet] RE: IHC on free floating slices of brain In-Reply-To: <0B8979A204680A42B93A52B486088CD93938636B14@CUAEXH1.GCU-MD.local> References: <0B8979A204680A42B93A52B486088CD93938636B14@CUAEXH1.GCU-MD.local> Message-ID: <1408539991691.71254@accuratediagnosticlabs.com> Hello Walter, Many years ago I did Timm's Silver stain on free-floating rat brain slices. The rats were perfused before removal and freezing of the brain on dry ice. I then would cut slices in a cryostat and float the sections in saline until ready to stain. I would imagine that it might be a bit costly to do IHC on free floating tissue sections because enough reagent and antibody solutions have to be used for each step. Not sure I can be of any help but email if you have any questions. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Walter Benton Sent: Tuesday, August 19, 2014 6:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on free floating slices of brain Does anyone have information they could share on performing IHC on whole mount, sledge microtome free floating slices of brain? A Neuropathologist colleague is interested in doing this. Thanks in advance for your help. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________ CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Aug 20 10:02:58 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Aug 20 10:03:11 2014 Subject: [Histonet] Vacuum Assisted Breast Biopsies Message-ID: For needle core breast biopsies, we cut several unstained slides - 3 levels and 2 unstained serials at each level. For vacuum assisted breast biopsies, which have much more tissue and many more blocks, we are finding that we are wasting a lot of slides cutting so many unstained. However, just yesterday, we had a case with 16 blocks, but two of them had DCIS with a question of microinvasion that would not have been confirmed without the serials for the IHC. So, our question is, how are you handling the vacuum assisted biopsies? Thanks for your help! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From tbraud <@t> holyredeemer.com Wed Aug 20 12:28:31 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Aug 20 12:28:34 2014 Subject: [Histonet] RE: great bone saw In-Reply-To: <20140820161144.A8BB51E80A1@trendmess-svr.holyredeemer.local> References: <20140820161144.A8BB51E80A1@trendmess-svr.holyredeemer.local> Message-ID: We LOVE the IMEB table top band saw for bone. It has a tiny footprint, easy to clean and operate, and it cuts incredible slices without any damage to the bone. It can use a regular blade for standard everyday bone sectioning, or a diamond blade for super delicate work. And no, I don't get any company kickback, though I'm open if someone from IMEB wants to talk a trip to the Fla Keys, LOL Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Is there a saw that you recommend that is precise and easy to handle that doesn't damage tissue greatly? Thank you so much for your help! Sincerely, Merissa --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From michang2014 <@t> gmail.com Wed Aug 20 13:15:23 2014 From: michang2014 <@t> gmail.com (Michelle Chang) Date: Wed Aug 20 13:15:27 2014 Subject: [Histonet] IHC on free floating slices of brain Message-ID: Dear Walter, In order to cut the tissue on the microtome, you need to first fixed the brain tissue (in rodents, normally, we perfused it using 4% PFA or 10% formalin), followed by post-fixation for a couple hours depending on the antibody of interest. Prior to cutting the tissue on the microtome, you have to put it in 20% to 30% sucrose (to get rid of water) solution for normally a day or two depending on how big the brain is (for mice brain minimum 1 day and rat brain minimum 2 days). Also note that if you have to do IHC on the free floating tissue, you will need to incubate the primary antibody and detection system for longer time as the tissue is thicker. PS: You will not be able to handle free floating section that is thinner than 25um. Let me know if you need more information. Michelle michang2014@gmail.com Date: Wed, 20 Aug 2014 13:06:54 +0000 From: Barbara Tibbs Subject: [Histonet] RE: IHC on free floating slices of brain To: Walter Benton , "histonet@lists.utsouthwestern.edu" Message-ID: <1408539991691.71254@accuratediagnosticlabs.com> Content-Type: text/plain; charset="iso-8859-1" Hello Walter, Many years ago I did Timm's Silver stain on free-floating rat brain slices. The rats were perfused before removal and freezing of the brain on dry ice. I then would cut slices in a cryostat and float the sections in saline until ready to stain. I would imagine that it might be a bit costly to do IHC on free floating tissue sections because enough reagent and antibody solutions have to be used for each step. Not sure I can be of any help but email if you have any questions. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.tibbs@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu < histonet-bounces@lists.utsouthwestern.edu> on behalf of Walter Benton < wbenton@cua.md> Sent: Tuesday, August 19, 2014 6:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on free floating slices of brain Does anyone have information they could share on performing IHC on whole mount, sledge microtome free floating slices of brain? A Neuropathologist colleague is interested in doing this. Thanks in advance for your help. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. From Lacie.Algeo <@t> providence.org Wed Aug 20 14:17:34 2014 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Wed Aug 20 14:18:10 2014 Subject: [Histonet] frozen sections Message-ID: <24C4B3C167E5694887AB594C7602CE3A0C34E1@WN35104.or.providence.org> Hi All, Thanks for all of your help with the slippery floors. I was also wondering which personnel labs are using for performing frozen sections? Lab Assistants? Histotechs? What are your minimal requirements? Do you classify frozen sections as high complexity testing? Thank you, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From michelecarr10 <@t> gmail.com Wed Aug 20 15:07:16 2014 From: michelecarr10 <@t> gmail.com (michele carr) Date: Wed Aug 20 15:07:22 2014 Subject: [Histonet] Traveling Histotech Message-ID: Hi everyone, I was wondering if you could recommend a reputable agency for traveling histotech. Thanks From cls71877 <@t> gmail.com Wed Aug 20 15:08:41 2014 From: cls71877 <@t> gmail.com (Cristi) Date: Wed Aug 20 15:08:57 2014 Subject: [Histonet] Traveling Histotech In-Reply-To: References: Message-ID: We used TechForce3 to cover my maternity leave and were happy with the services. Sent from my iPhone > On Aug 20, 2014, at 1:07 PM, michele carr wrote: > > Hi everyone, I was wondering if you could recommend a reputable agency for > traveling histotech. > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Liz.Drinkall <@t> nmhs.org Thu Aug 21 09:10:15 2014 From: Liz.Drinkall <@t> nmhs.org (Drinkall, Liz) Date: Thu Aug 21 09:10:27 2014 Subject: [Histonet] Re: On the lighter side... Message-ID: 8 years total, 6 years registered. Liz Drinkall,?HTL (ASCP) CM Methodist Hospital Histology Lab/Nebraska Collaborative Lab 8303 Dodge St. Omaha, NE 68114 (402)354-4572/(402)354-7974 This message and any included attachments are from Nebraska Methodist Health System and its affiliates and are intended only for the addressee. The message may contain privileged, confidential and/or proprietary information intended only for the person(s) named. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Nebraska Methodist Health System and its affiliates in Omaha, Nebraska, U.S.A at (402)354-2280. From rmire <@t> cvpath.org Thu Aug 21 11:03:53 2014 From: rmire <@t> cvpath.org (Ronda Mire) Date: Thu Aug 21 11:04:17 2014 Subject: [Histonet] (no subject) Message-ID: <000001cfbd59$89e810e0$9db832a0$@org> Hello Netters, Anyone happen to know what the recommended humidity level range is for Methylmethacrylate infiltration and embedding is? How about any publications that covers this topic? Thanks Ronda Mire Laboratory Manager/Chief Technologist CVPath Institute 19 Firstfield Rd Gaithersburg, MD 20878 301-208-3570 x112 rmire@cvpath.org From bburnett <@t> CapeCodHealth.org Thu Aug 21 12:10:38 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Thu Aug 21 12:10:48 2014 Subject: [Histonet] IHC QC forms: In-Reply-To: <761E2B5697F795489C8710BCC72141FF3679120A@ex07.net.ucsf.edu> References: <67F2DF4F-D7C5-4E07-A2B1-41857796943D@gmail.com>, <761E2B5697F795489C8710BCC72141FF3679120A@ex07.net.ucsf.edu> Message-ID: Thanks for your input. We are having the same issues with our IHC QA/QC and monthly repeat count. The pathologists place the IHC orders through PowerPath, so I'm hoping that we can create a "Repeat" code in PowerPath to keep track of them. Do you know how it was set up in your ordering system? Thanks again, Brandy Burnett, HTL Cape Cod Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Thursday, August 07, 2014 2:26 PM To: 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: We only check and document control slide quality and need for repeat due to control failure. The pathologist reviewing the slides will send a QC form to us if there is a problem with case slides. Plus thye can enter a repeat for a poor stain as a ""repeat" and we can track the number of repeats in the system. It is actually one of our QA monitors and review of repeats is documented in our quarterly QA review meeting. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, August 07, 2014 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC forms: Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From Jeff.Howery <@t> kadlecmed.org Thu Aug 21 15:06:32 2014 From: Jeff.Howery <@t> kadlecmed.org (Howery, Jeff L) Date: Thu Aug 21 15:06:38 2014 Subject: [Histonet] Job Opening Message-ID: Hi my name is Jeff and I am the Pathology Supervisor here at Kadlec Regional Medical Center Richland, WA. I just had a Histologist resign today as she is moving to Florida to be closer to her Parents. I am looking for someone to fill this position that has Immuno experience and I would prefer someone ASCP/QIHC. This posting will not take place on our web site till next week sometime. Kadlec is a 270 bed facility with an expansion taking place next year to over 300 beds. Last year we did almost 13,000 cases and growing every year. Richland is Part of the Tri-Cities Pasco, Kennewick, and Richland with the area population of about 250,000 both cities and rural area. The Columbia, Snake, and Yakima Rivers converge here so there are boating and fishing opportunities. We are 2 hours from Spokane and 3 ? hours from Seattle with snow skiing along the Cascade mountain range and in Spokane. Let me know if you have any question. Regards, Jeff From LSebree <@t> uwhealth.org Thu Aug 21 16:19:14 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Thu Aug 21 16:19:19 2014 Subject: [Histonet] IHC QC forms: In-Reply-To: References: <67F2DF4F-D7C5-4E07-A2B1-41857796943D@gmail.com>, <761E2B5697F795489C8710BCC72141FF3679120A@ex07.net.ucsf.edu> Message-ID: <77DD817201982748BC67D7960F2F76AF0C8E1C@UWHC-MBX12.uwhis.hosp.wisc.edu> Brandy, We are completely paperless now so we created a code called "RPTIHC" that had no billing charge associated with it. The pathologist then puts what antibody/antibodies need repeating in the instruction box of the repeat code. They also indicate the reason for the repeat(s). It populates nicely to the worklist that gets printed; works well for us. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, August 21, 2014 12:11 PM To: Morken, Timothy; 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: Thanks for your input. We are having the same issues with our IHC QA/QC and monthly repeat count. The pathologists place the IHC orders through PowerPath, so I'm hoping that we can create a "Repeat" code in PowerPath to keep track of them. Do you know how it was set up in your ordering system? Thanks again, Brandy Burnett, HTL Cape Cod Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Thursday, August 07, 2014 2:26 PM To: 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: We only check and document control slide quality and need for repeat due to control failure. The pathologist reviewing the slides will send a QC form to us if there is a problem with case slides. Plus thye can enter a repeat for a poor stain as a ""repeat" and we can track the number of repeats in the system. It is actually one of our QA monitors and review of repeats is documented in our quarterly QA review meeting. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, August 07, 2014 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC forms: Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bburnett <@t> CapeCodHealth.org Fri Aug 22 07:20:46 2014 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Fri Aug 22 07:20:56 2014 Subject: [Histonet] IHC QC forms: In-Reply-To: <77DD817201982748BC67D7960F2F76AF0C8E1C@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <67F2DF4F-D7C5-4E07-A2B1-41857796943D@gmail.com>, <761E2B5697F795489C8710BCC72141FF3679120A@ex07.net.ucsf.edu> , <77DD817201982748BC67D7960F2F76AF0C8E1C@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Linda~ We would love to be completely paperless eventually. We are in the process of interfacing our instruments with PowerPath and this is where we are having a problem developing a code for the repeats. Thanks again for your advice, every little bit helps! Sincerely, Brandy Burnett, HTL Cape Cod Hospital ________________________________________ From: Sebree Linda A [LSebree@uwhealth.org] Sent: Thursday, August 21, 2014 5:19 PM To: Burnett, Brandy; 'Morken, Timothy'; 'Jb'; 'Histonet' Subject: RE: [Histonet] IHC QC forms: Brandy, We are completely paperless now so we created a code called "RPTIHC" that had no billing charge associated with it. The pathologist then puts what antibody/antibodies need repeating in the instruction box of the repeat code. They also indicate the reason for the repeat(s). It populates nicely to the worklist that gets printed; works well for us. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, August 21, 2014 12:11 PM To: Morken, Timothy; 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: Thanks for your input. We are having the same issues with our IHC QA/QC and monthly repeat count. The pathologists place the IHC orders through PowerPath, so I'm hoping that we can create a "Repeat" code in PowerPath to keep track of them. Do you know how it was set up in your ordering system? Thanks again, Brandy Burnett, HTL Cape Cod Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Thursday, August 07, 2014 2:26 PM To: 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: We only check and document control slide quality and need for repeat due to control failure. The pathologist reviewing the slides will send a QC form to us if there is a problem with case slides. Plus thye can enter a repeat for a poor stain as a ""repeat" and we can track the number of repeats in the system. It is actually one of our QA monitors and review of repeats is documented in our quarterly QA review meeting. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, August 07, 2014 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC forms: Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From kgrobert <@t> rci.rutgers.edu Fri Aug 22 09:44:25 2014 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Aug 22 09:44:29 2014 Subject: [Histonet] Sakura Tissue Tek TEC manual? Message-ID: We just got one of these embedding centers at auction. This is model 4710, but it didn't come with a user manual. An online search turned up manuals for the TEC 4 and 5, but not this one. Would anyone happen to have one, and if so, any chance you can scan it and email it to me? (No rush, though. We have pretty much figured it out, but it would be good to have one anyway.) Thank you so much! Kathleen Roberts Principal Lab Technician Histopathology Lab Office of Translational Sciences Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From Lacie.Algeo <@t> providence.org Fri Aug 22 12:17:25 2014 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Fri Aug 22 12:17:48 2014 Subject: [Histonet] IHC QC forms: Message-ID: <24C4B3C167E5694887AB594C7602CE3A0CEAB9@WN35104.or.providence.org> Hi Brandy, Our QC program indicates that all slides are QC'd by a tech prior to leaving the lab. Once, QC is done, the tech delineates between the patient tissue and control. This indicates to the lab assistant that they are ready to be distributed. Stain quality is also 'assumed' through the report disclaimer after the case is read out by the pathologist which includes the statement: Ancillary studies were performed on this case with appropriate controls showing appropriate reactivity. Where we do document is when a stain does not work as expected. At the time of tech QC, if the stain did not work, we print the run log, attach the bad slide with comments and perform a repeat following investigation into why it did not work. The repeat results and initials are noted on the log and this is filed in a binder. This saves us a lot of time. If the pathologist has an issue with a stain, they submit a QI issue log to me and I research the issue. Hope this helps. Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, August 22, 2014 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: IHC QC forms: (Burnett, Brandy) 2. Job Opening (Howery, Jeff L) 3. RE: IHC QC forms: (Sebree Linda A) 4. RE: IHC QC forms: (Burnett, Brandy) 5. Sakura Tissue Tek TEC manual? (Kathleen Roberts) ---------------------------------------------------------------------- Message: 1 Date: Thu, 21 Aug 2014 17:10:38 +0000 From: "Burnett, Brandy" Subject: RE: [Histonet] IHC QC forms: To: "Morken, Timothy" , "'Jb'" , Histonet Message-ID: Content-Type: text/plain; charset="us-ascii" Thanks for your input. We are having the same issues with our IHC QA/QC and monthly repeat count. The pathologists place the IHC orders through PowerPath, so I'm hoping that we can create a "Repeat" code in PowerPath to keep track of them. Do you know how it was set up in your ordering system? Thanks again, Brandy Burnett, HTL Cape Cod Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Thursday, August 07, 2014 2:26 PM To: 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: We only check and document control slide quality and need for repeat due to control failure. The pathologist reviewing the slides will send a QC form to us if there is a problem with case slides. Plus thye can enter a repeat for a poor stain as a ""repeat" and we can track the number of repeats in the system. It is actually one of our QA monitors and review of repeats is documented in our quarterly QA review meeting. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, August 07, 2014 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC forms: Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org ------------------------------ Message: 2 Date: Thu, 21 Aug 2014 13:06:32 -0700 From: "Howery, Jeff L" Subject: [Histonet] Job Opening To: "'Histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi my name is Jeff and I am the Pathology Supervisor here at Kadlec Regional Medical Center Richland, WA. I just had a Histologist resign today as she is moving to Florida to be closer to her Parents. I am looking for someone to fill this position that has Immuno experience and I would prefer someone ASCP/QIHC. This posting will not take place on our web site till next week sometime. Kadlec is a 270 bed facility with an expansion taking place next year to over 300 beds. Last year we did almost 13,000 cases and growing every year. Richland is Part of the Tri-Cities Pasco, Kennewick, and Richland with the area population of about 250,000 both cities and rural area. The Columbia, Snake, and Yakima Rivers converge here so there are boating and fishing opportunities. We are 2 hours from Spokane and 3 ? hours from Seattle with snow skiing along the Cascade mountain range and in Spokane. Let me know if you have any question. Regards, Jeff ------------------------------ Message: 3 Date: Thu, 21 Aug 2014 21:19:14 +0000 From: Sebree Linda A Subject: RE: [Histonet] IHC QC forms: To: "'Burnett, Brandy'" , "'Morken, Timothy'" , 'Jb' , 'Histonet' Message-ID: <77DD817201982748BC67D7960F2F76AF0C8E1C@UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" Brandy, We are completely paperless now so we created a code called "RPTIHC" that had no billing charge associated with it. The pathologist then puts what antibody/antibodies need repeating in the instruction box of the repeat code. They also indicate the reason for the repeat(s). It populates nicely to the worklist that gets printed; works well for us. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, August 21, 2014 12:11 PM To: Morken, Timothy; 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: Thanks for your input. We are having the same issues with our IHC QA/QC and monthly repeat count. The pathologists place the IHC orders through PowerPath, so I'm hoping that we can create a "Repeat" code in PowerPath to keep track of them. Do you know how it was set up in your ordering system? Thanks again, Brandy Burnett, HTL Cape Cod Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Thursday, August 07, 2014 2:26 PM To: 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: We only check and document control slide quality and need for repeat due to control failure. The pathologist reviewing the slides will send a QC form to us if there is a problem with case slides. Plus thye can enter a repeat for a poor stain as a ""repeat" and we can track the number of repeats in the system. It is actually one of our QA monitors and review of repeats is documented in our quarterly QA review meeting. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, August 07, 2014 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC forms: Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 22 Aug 2014 12:20:46 +0000 From: "Burnett, Brandy" Subject: RE: [Histonet] IHC QC forms: To: Sebree Linda A , "'Morken, Timothy'" , "'Jb'" , "'Histonet'" Message-ID: Content-Type: text/plain; charset="us-ascii" Linda~ We would love to be completely paperless eventually. We are in the process of interfacing our instruments with PowerPath and this is where we are having a problem developing a code for the repeats. Thanks again for your advice, every little bit helps! Sincerely, Brandy Burnett, HTL Cape Cod Hospital ________________________________________ From: Sebree Linda A [LSebree@uwhealth.org] Sent: Thursday, August 21, 2014 5:19 PM To: Burnett, Brandy; 'Morken, Timothy'; 'Jb'; 'Histonet' Subject: RE: [Histonet] IHC QC forms: Brandy, We are completely paperless now so we created a code called "RPTIHC" that had no billing charge associated with it. The pathologist then puts what antibody/antibodies need repeating in the instruction box of the repeat code. They also indicate the reason for the repeat(s). It populates nicely to the worklist that gets printed; works well for us. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, August 21, 2014 12:11 PM To: Morken, Timothy; 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: Thanks for your input. We are having the same issues with our IHC QA/QC and monthly repeat count. The pathologists place the IHC orders through PowerPath, so I'm hoping that we can create a "Repeat" code in PowerPath to keep track of them. Do you know how it was set up in your ordering system? Thanks again, Brandy Burnett, HTL Cape Cod Hospital ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Morken, Timothy [Timothy.Morken@ucsfmedctr.org] Sent: Thursday, August 07, 2014 2:26 PM To: 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: We only check and document control slide quality and need for repeat due to control failure. The pathologist reviewing the slides will send a QC form to us if there is a problem with case slides. Plus thye can enter a repeat for a poor stain as a ""repeat" and we can track the number of repeats in the system. It is actually one of our QA monitors and review of repeats is documented in our quarterly QA review meeting. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, August 07, 2014 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC forms: Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org ------------------------------ Message: 5 Date: Fri, 22 Aug 2014 10:44:25 -0400 (EDT) From: "Kathleen Roberts" Subject: [Histonet] Sakura Tissue Tek TEC manual? To: "histonet" Message-ID: Content-Type: text/plain;charset=iso-8859-1 We just got one of these embedding centers at auction. This is model 4710, but it didn't come with a user manual. An online search turned up manuals for the TEC 4 and 5, but not this one. Would anyone happen to have one, and if so, any chance you can scan it and email it to me? (No rush, though. We have pretty much figured it out, but it would be good to have one anyway.) Thank you so much! Kathleen Roberts Principal Lab Technician Histopathology Lab Office of Translational Sciences Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 31 ***************************************** ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From kgrobert <@t> rci.rutgers.edu Fri Aug 22 12:37:32 2014 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Fri Aug 22 12:37:36 2014 Subject: [Histonet] Re: User manual for Sakura Tissue Tek TEC 4710 In-Reply-To: <2C7597ABF5467247BE159D763E27F839D4B9364A1D@PROMETHEUS.medequip.local> References: <2C7597ABF5467247BE159D763E27F839D4B9364A1D@PROMETHEUS.medequip.local> Message-ID: <7c8b4d812b9ab76a85b87be794be7490.squirrel@webmail.rci.rutgers.edu> Oho! Thank you very much, Jim! To the rest of Histonet, I withdraw my request for a manual. Now that I have that missing piece of information, I know just where to get it. Thanks anyway and have a great weekend! Kathleen Roberts Principal Lab Technician Histopathology Lab Office of Translational Sciences Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 > 4710 is a Tec 4 > > > Jim Anzaldi, > [cid:image004.jpg@01CFBE0A.A4BB9160] > Warehouse/Service Manager > Medical Equipment Source > 364 Mars Valencia Road > Mars PA 16046 > > Service 724-369-1604 > Fax 724-625-2235 > [cid:image003.jpg@01CFBE0A.A2768360] > Confidentiality Warning: This e-mail contains information intended only > for the use of the individual or entity named above. If the reader of this > e-mail is not the intended recipient or the employee or agent responsible > for delivering it to the intended recipient, any dissemination, > publication or copying of this e-mail is strictly prohibited. The sender > does not accept any responsibility for any loss, disruption or damage to > your data or computer system that may occur while using data contained in, > or transmitted with, this e-mail. If you have received this e-mail in > error, please immediately notify us by return e-mail. Thank you > > From pruegghm <@t> hotmail.com Fri Aug 22 14:47:58 2014 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Fri Aug 22 14:48:02 2014 Subject: [Histonet] Microtome for HS Biotech Class Message-ID: Dear Histo Peeps, I am on the advisory board for a local high school Biotech class and have been helping them process, embed, cut, and stain tissue (they use chicken livers can't use human). My husband fixed up an old black AO microtome for them a few years ago so they actually cut sections. This year the teacher contacted me and told me that her usual class of about 20-24 has gone to 32 and she doesn't think she will have enough time to teach everyone to cut a section with just one microtome. She asked me if I knew anyone locally they could borrow a microtome from, so I have been asking around but since I am retired I do not have access to many resources anymore. I thought I would put this out to our histo community especially if there is someone local in Colorado that could help us out. I also thought that one of the equipment vendors like Rankin might be able to help out. This is a Cherry Creek High School (Overland) and the teacher said the school would give out receipts for tax purposes for any donations received. Thank you all for considering this request. Best regards, Patsy PS Maybe our past and new Region VII directors (Janet and Jane) could help with this??? Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From akbitting <@t> geisinger.edu Mon Aug 25 07:39:56 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Mon Aug 25 07:40:03 2014 Subject: [Histonet] microtome laceration not an exposure Message-ID: <77F52EFAB8B1694B885E277C48FCD0F67BA47696@GHSEXMBX1W8K1V.geisinger.edu> Does anyone have something that I can show to nursing to explain why a microtome laceration is not an exposure? I can't get the "fixed tissue" explanation to sink in. Anyone else had issues with this? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From Joyce.Fortin <@t> uhsinc.com Mon Aug 25 09:54:45 2014 From: Joyce.Fortin <@t> uhsinc.com (Fortin, Joyce) Date: Mon Aug 25 09:59:42 2014 Subject: [Histonet] Placentas Message-ID: Would anyone be willing to share their policy on how to handle placentas? I have public health asking. Thank you! UHS of Delaware, Inc. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited, and may be punishable by law. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. From akbitting <@t> geisinger.edu Mon Aug 25 10:42:02 2014 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Mon Aug 25 10:43:55 2014 Subject: [Histonet] PAS kit Message-ID: <77F52EFAB8B1694B885E277C48FCD0F67BA48ACA@GHSEXMBX1W8K1V.geisinger.edu> Is anyone else in Histoland having issues with the Ventana PAS kits? Particularly, crystals laying on top of the tissue after staining. If so, please contact me off line. Thanks, Angie Angela K. Bitting, HT(ASCP),QIHC Technical Specialist for Special Stains/ IHC/ISH Geisinger Health System 100 N. Academy Avenue Danville, PA 17822-2300 t. 570-214-9634 f. 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From Kim.Kolman <@t> va.gov Mon Aug 25 12:06:02 2014 From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.) Date: Mon Aug 25 12:14:21 2014 Subject: [Histonet] slide filing cabinets Message-ID: <9C32F30B6662D74A8419DDDB7E66656A091E8607@VHAV15MSGA1.v15.med.va.gov> Dear Histo-netters: I'm looking to re-arrange my paraffin block storage; currently the plastic block cabinets are on a shelving unit. Does anyone have any experience with how well the old Tissue Tek plastic 6-drawer block cabinets stack - on top of each other? Can I safely do a stack of 5? And will they hold up well? Thanks in advance. Kim Kim Kolman Kimberly D. Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center Histology - 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000x52537 Fax: 913-758-4193 From HornHV <@t> archildrens.org Mon Aug 25 12:28:37 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Aug 25 12:28:41 2014 Subject: [Histonet] RE: slide filing cabinets In-Reply-To: <9C32F30B6662D74A8419DDDB7E66656A091E8607@VHAV15MSGA1.v15.med.va.gov> References: <9C32F30B6662D74A8419DDDB7E66656A091E8607@VHAV15MSGA1.v15.med.va.gov> Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFE43@EVS1.archildrens.org> We have no problems stacking the block cabinets from Tissue-Tek. 5 high will not be a problem. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kolman, Kimberly D. Sent: Monday, August 25, 2014 12:06 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide filing cabinets Dear Histo-netters: I'm looking to re-arrange my paraffin block storage; currently the plastic block cabinets are on a shelving unit. Does anyone have any experience with how well the old Tissue Tek plastic 6-drawer block cabinets stack - on top of each other? Can I safely do a stack of 5? And will they hold up well? Thanks in advance. Kim Kim Kolman Kimberly D. Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center Histology - 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000x52537 Fax: 913-758-4193 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Wanda.Smith <@t> HCAhealthcare.com Mon Aug 25 12:39:02 2014 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Mon Aug 25 12:40:32 2014 Subject: [Histonet] RE: slide filing cabinets In-Reply-To: <9C32F30B6662D74A8419DDDB7E66656A091E8607@VHAV15MSGA1.v15.med.va.gov> References: <9C32F30B6662D74A8419DDDB7E66656A091E8607@VHAV15MSGA1.v15.med.va.gov> Message-ID: <9E2D36CE2D7CBA4A94D9B22E8328A3BA27FF045DD7@NADCWPMSGCMS03.hca.corpad.net> I have been stacking the TT II plastic block file cabinets 5 high for years and I have never had a problem with them. Yes, they do hold up well! W WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kolman, Kimberly D. Sent: Monday, August 25, 2014 1:06 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide filing cabinets Dear Histo-netters: I'm looking to re-arrange my paraffin block storage; currently the plastic block cabinets are on a shelving unit. Does anyone have any experience with how well the old Tissue Tek plastic 6-drawer block cabinets stack - on top of each other? Can I safely do a stack of 5? And will they hold up well? Thanks in advance. Kim Kim Kolman Kimberly D. Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center Histology - 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000x52537 Fax: 913-758-4193 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Aug 25 14:55:48 2014 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Aug 25 14:55:56 2014 Subject: [Histonet] BRAF by immunohistochemistry Message-ID: <25A4DE08332B19499904459F00AAACB719E29EFE51@EVS1.archildrens.org> Does anyone know of a place we can send a case to for BRAF immunohistochemistry? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From anolan <@t> prometheushealthcare.com Mon Aug 25 15:25:35 2014 From: anolan <@t> prometheushealthcare.com (anolan@prometheushealthcare.com) Date: Mon Aug 25 15:24:02 2014 Subject: [Histonet] histology openings Message-ID: <00c101cfc0a2$bbd1af70$33750e50$@prometheushealthcare.com> I have competitive histology openings in the following states with a growing reference lab, ranging from leads-supervisors. Looking at well experienced HT(ASCP) recognized professionals only. Please forward up to date and detailed resumes to the email address below for further consideration. Manitowoc, WI Phoenix, AZ Dallas, TX Irving, TX Rye brook, NY Zanesville, OH Taylor Rinaldi Recruiter Prometheus Healthcare Direct Line (407) 334-4438 Office (301) 693-9057 Fax (301) 368-2478 Taylor@prometheushealthcare.com www.prometheushealthcare.com From jpalmer <@t> obi.edu.au Tue Aug 26 02:05:07 2014 From: jpalmer <@t> obi.edu.au (Jason Palmer) Date: Tue Aug 26 02:05:20 2014 Subject: [Histonet] protein A problem with Staph aureus immunostaining In-Reply-To: <20140825170928.54687AA048@mx1s.medstv.unimelb.edu.au> Message-ID: <740807818.88014.1409036707670.JavaMail.root@zstore.medstv.unimelb.edu.au> Hi all, ? A question re. immunostaining of bacterial antigens.? We are trying to stain cultured MRSA (Staph. aureus) with an antibody to PBP2a, which is the protein that confers antibiotic resistance to these bugs.? So far we are finding non-specific binding of our antibodies to protein A is a real problem (or at least we assume that protein A is the problem).? Primary is rabbit anti PBP2a and we follow this with a Cy3- conjugated anti rabbit IgG.? We have tried normal serum blocking (10% rabbit) before the primary, as well as an Fc fragment of rabbit IgG as a more specific blocker of protein A before the primary, but so far without much success; we see labe ling with our primary, with an isotype control and also with diluent alone.? So it would seem that both our primary and?secondary is binding to protein A non-specifically. ? Next things to try are increasing the concentration of the Fc fragment blocking? (was about 100ug/ml the first time) and also to try an Fab fragment as a secondary, rather than the whole IgG we currently use.? Does anybody have any other suggestions as to what we might try to get on top of this problem?? I have only ever immunostained mammalian cells and tissues before, so this is my first time with bacteria.? The dyes I'm using (Cy3 and DAPI) photobleach very easily for some reason with these bugs compared with your average mammalian cells? ? Thanks for any suggestions, ? Jason ? Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jpalmer@obi.edu.au From rsrichmond <@t> gmail.com Tue Aug 26 07:39:21 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Aug 26 07:39:30 2014 Subject: [Histonet] Re: Placentas Message-ID: Joyce Fortin at UHSINC (where?) asks: >>Would anyone be willing to share their policy on how to handle placentas? I have public health asking.<< There are a number of standard references on how to gross a placenta - it's your pathologist's responsibility to get this information for you. You should not be expected to gross a placenta without being shown how, and the pathologist should be very slow to delegate grossing twin placentas. I want four cassettes for a normal singleton placenta. The first is for the membrane roll, which somebody needs to show you how to do, with a wooden applicator stick and a straight pin. The second is for two or three sections of the cord segment. The third is for the fetal surface, one piece with great vessels and one piece from the margin. The fourth is for two or three separate pieces of the maternal surface. Don't try to sample fetal and maternal surface together in one big piece. Swish the placenta pieces vigorously through formalin to get the blood out of them so they won't soil the processor fixative. Bob Richmond Samurai Pathologist Maryville TN From sbaldwin <@t> mhhcc.org Tue Aug 26 07:57:11 2014 From: sbaldwin <@t> mhhcc.org (Baldwin, Kathy) Date: Tue Aug 26 07:57:22 2014 Subject: [Histonet] new codes for Medicare Message-ID: Hi Histonetters Does anyone have any advice on what to do with this? We already have our codes changed to the G code but Medicare is withholding re-imbursements (from what I understand) Wanting these 3 Group2 codes to be attached. I guess I need to contact the physicians get them educated on this but in the meantime try to get these codes applied to the reports that are in holding?? Any advice?? None of these V codes would really allow for H Pylori, right? CPT/HCPCS Codes G0461 - Immunohistochemistry or immunocytochemistry, per specimen; first single or multiplex antibody stain; G0462 - Immunohistochemistry or immunocytochemistry, per specimen; each additional single or multiplex antibody stain (list separately in addition to code for primary procedure); They are covered for the following diagnosis codes: Group 2 Paragraph : 81301, G0461, G0462 Group 2 codes: V16.0 FAMILY HISTORY OF MALIGNANT NEOPLASM OF GASTROINTESTINAL TRACT V84.04 GENETIC SUSCEPTIBILITY TO MALIGNANT NEOPLASM OF ENDOMETRIUM V84.09 GENETIC SUSCEPTIBILITY TO OTHER MALIGNANT NEOPLASM S Kathy Baldwin Histology/Cytology Supervisor PH. 812-996-0210, Fax 812-996-0232 sbaldwin@mhhcc.org :) "Christ's healing mission of compassion empowers us to be for others through Quality and Excellence" Vision Statement: We are committed to being the preferred health and wellness provider; transforming lives through faith based, compassionate care. From blayjorge <@t> gmail.com Tue Aug 26 08:52:37 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Tue Aug 26 08:52:43 2014 Subject: [Histonet] Cleaning lenses of light microscopes Message-ID: Hello: I am trying to clean lenses of teaching light microscopes. In addition to dusting lenses the lenses and inner parts carefully, there are numerous, sometimes conflicting, recommendations re. liquids to use. Do you have any? Also, some companies sell "lens cleaner". Do you know what are those products made out off. Any recommendations (positive or negative)? If you have any comments, please feel free to send them to my email: blayjorge@gmail.com Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From JWatson <@t> gnf.org Tue Aug 26 09:40:29 2014 From: JWatson <@t> gnf.org (James Watson) Date: Tue Aug 26 09:40:36 2014 Subject: [Histonet] Cleaning lenses of light microscopes In-Reply-To: References: Message-ID: Jorge, I service microscopes for 8 years at 2 companies. One company (a Zeiss dealership) used Glass Plus, the other company used regular Windex (not extra strength). Both companies used them for years and saw no damage to the lenses, many companies will tell you that glass cleaners will damage the coatings on the lenses. The secret is to start with a cleaner dampened cotton tipped applicator(CTA), clean the lens then dry immediately with a clean CTA. Then blow off the lens, we used puffers not canned air since canned air contain components that can leave a film on the lens. Do not use acetone or xylene, they will dissolve the cement that holds the lens in place. Jamie James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jorge A. Santiago-Blay Sent: Tuesday, August 26, 2014 6:53 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Cleaning lenses of light microscopes Hello: I am trying to clean lenses of teaching light microscopes. In addition to dusting lenses the lenses and inner parts carefully, there are numerous, sometimes conflicting, recommendations re. liquids to use. Do you have any? Also, some companies sell "lens cleaner". Do you know what are those products made out off. Any recommendations (positive or negative)? If you have any comments, please feel free to send them to my email: blayjorge@gmail.com Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Aug 26 09:49:34 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 26 09:52:27 2014 Subject: [Histonet] Cleaning lenses of light microscopes In-Reply-To: References: Message-ID: <1409064574.6314.YahooMailNeo@web120405.mail.ne1.yahoo.com> Hi Jorge: Cleaning objective lenses has to be limited to the external surfaces (the front lens close to the slide and the back lens close to the microscope tube). Trying to clean the interior lenses is not recommended because it will involve disassembling the different duplets and triplets it may contain and sometimes putting them back is very tricky. Before trying to clean the surfaces of the lenses you have to eliminate any and all dust particles because if you do not do that you may scratch the surface. There are many?pressurized air containers used for surface clean computer keyboards that are perfectly suitable for that purpose. The best liquid I have found to clean lenses is 70% isopropyl alcohol sold everywhere as "rubbing alcohol" and you should use Q-Tips to apply it. First wet one Q-Tip, rub it against the cleaned?lens and later remove the alcohol with another and use as many as you need to leave the lens completely dry. Never use the Q-Tips more than once. You should use a small flashlight to control the status of the lens as to its cleanliness. Oculars are a different issue because you have to remove the 2 lens holders (usually a single lens each, unless you have "Compensation" or?"Periplan" or high magnification oculars (above 10X) in which case you may have a "duplet" on the top. If you lose track of which lens goes up or down, look at the fixed inner diaphragm in the ocular and notice that it has a border to hold it in place; that border is closest to the? lower lens. "Lens cleaners" have all proprietary formulas and some even are green tinted?to differentiate from others but rubbing alcohol will do. Avoid using xylene (although in cases when there is a large immersion oil smooch on the front?lens of the immersion objective it is absolutely required), otherwise xylene may "sip" into the doublets and with time affect the cement between the lenses ruining the objective. I hope this will help you in your endeavor Ren? J.? On Tuesday, August 26, 2014 9:53 AM, Jorge A. Santiago-Blay wrote: Hello: I am trying to clean lenses of teaching light microscopes. In addition to dusting lenses the lenses and inner parts carefully, there are numerous, sometimes conflicting, recommendations re. liquids to use. Do you have any? Also, some companies sell "lens cleaner". Do you know what are those products made out off. Any recommendations (positive or negative)? If you have any comments, please feel free to send them to my email: blayjorge@gmail.com? Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Tue Aug 26 10:22:11 2014 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Aug 26 10:22:23 2014 Subject: [Histonet] GHS Labeling Message-ID: <12ECD7346266D74691EC2BFC75285E452F4D130B@BFL323E10.pathmdlabs.local> Does anyone know of a source that sells a "poster" that can be hung on the wall that shows all of the pictograms for the new Globally Harmonized System (GHS) labeling system? I would like a "master" list that can be referred to. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From Nancy_Schmitt <@t> pa-ucl.com Tue Aug 26 11:39:27 2014 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue Aug 26 11:39:38 2014 Subject: [Histonet] how long to keep IHC validation materials Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3601157735F5@PEITHA.wad.pa-ucl.com> We are having a discussion on how long to keep the slides for IHC validation? As long as you have the instrument? Or can the slides be disposed of at some given time (5years?)? Thank you for your input- Nancy Schmitt MLT, HT(ASCP) Histology Coordinator United Clinical Laboratories visit us at www.uclaccess.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Kim.Kolman <@t> va.gov Tue Aug 26 11:57:09 2014 From: Kim.Kolman <@t> va.gov (Kolman, Kimberly D.) Date: Tue Aug 26 11:57:50 2014 Subject: [Histonet] RE: [EXTERNAL] RE: slide filing cabinets Message-ID: <9C32F30B6662D74A8419DDDB7E66656A091E860A@VHAV15MSGA1.v15.med.va.gov> Thanks all; now if I can get someone to give me the go-ahead on space, I can get to stacking away! -----Original Message----- From: Wanda.Smith@HCAhealthcare.com [mailto:Wanda.Smith@HCAhealthcare.com] Sent: Monday, August 25, 2014 12:39 PM To: Kolman, Kimberly D.; Histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: slide filing cabinets I have been stacking the TT II plastic block file cabinets 5 high for years and I have never had a problem with them. Yes, they do hold up well! W WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kolman, Kimberly D. Sent: Monday, August 25, 2014 1:06 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide filing cabinets Dear Histo-netters: I'm looking to re-arrange my paraffin block storage; currently the plastic block cabinets are on a shelving unit. Does anyone have any experience with how well the old Tissue Tek plastic 6-drawer block cabinets stack - on top of each other? Can I safely do a stack of 5? And will they hold up well? Thanks in advance. Kim Kim Kolman Kimberly D. Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center Histology - 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000x52537 Fax: 913-758-4193 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schaundrawalton <@t> yahoo.com Tue Aug 26 12:06:00 2014 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Tue Aug 26 12:06:06 2014 Subject: [Histonet] Processing baskets for Peloris Message-ID: Hey histoland! Anybody know where I could purchase some gently used divided racks for a Leica Peloris machine? They are very expensive new. TIA Schaundra Walton BS, HTL (ASCP) Histology Supervisor PathGroup Nashville, TN From DArguello <@t> che-east.org Tue Aug 26 12:43:57 2014 From: DArguello <@t> che-east.org (Arguello, Daniel) Date: Tue Aug 26 12:44:31 2014 Subject: [Histonet] HPV Validation from SurePath Pap test vials Message-ID: <483E0ED08CFA9742BF39DCB864251A701125298C95@CHEXCMS02.one.ads.che.org> Dear friends at HistoNet, I'd appreciate if any of you can share a protocol in validating HPV from Surepath Pap test vials. I have an inquiry from a client and would appreciate your insight. Regards, Daniel Arg?ello BS, CT(ASCP)CM Cytopathology Services Supervisor Saint Francis Healthcare 701 N. Clayton St. Wilmington, DE 19805 302-575-8119 darguello@che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Thomas.Jasper <@t> deaconess.com Tue Aug 26 15:54:14 2014 From: Thomas.Jasper <@t> deaconess.com (Thomas Jasper) Date: Tue Aug 26 15:54:23 2014 Subject: [Histonet] Looking for suggestions, ideas Message-ID: Hi Folks, Here is our situation. In the not too distant future we will be receiving additional specimens from 3 dermatologists. What they need is to have these specimens processed, embedded, cut and H/E stained. Once we've got the slides coverslipped they are to be sent back the dermatologists. We are not doing the interpretation. What I'm looking for is an idea about getting these into our LIS (PowerPath) and designating them for return to this group. We should be able to capture the TC and need to account for the workload. I'm thinking we could prefix them differently at the time of accessioning and then create something in the LIS attached to that designation which only generates a TC. I'm wondering if anyone else does anything like this? I'm also interested in having this differing designation for efficient workflow. I don't want any of these making their way to our pathologists. I've thought of identification by color of block and a new prefix. Just not sure if/what others are doing, how difficult to implement for an LIS standpoint (separation of TC and PC) and any other considerations. Thanks, I know I've got all the superior minds out there. Your thoughts are greatly appreciated. Tom Jasper Thomas Jasper HT (ASCP) BAS AP/CP Supervisor Deaconess Hospital 600 Mary Street Evansville, IN 47747 Thomas.jasper@deaconess.com 812-450-2485 From robrankin <@t> rankinbiomed.com Tue Aug 26 16:52:24 2014 From: robrankin <@t> rankinbiomed.com (Rob Rankin) Date: Tue Aug 26 16:52:29 2014 Subject: [Histonet] Re: Histonet Digest, Vol 129, Issue 34 In-Reply-To: <53fcbd5c.a34b3c0a.6c75.3162SMTPIN_ADDED_MISSING@mx.google.com> References: <53fcbd5c.a34b3c0a.6c75.3162SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <64C48E4F-75BE-48BC-AF49-5121D393AC90@rankinbiomed.com> S > > > > ------------------------------ > > M > > > Dear Histo-netters: > > > > I'm looking to re-arrange my paraffin block storage; currently the plastic block cabinets are on a shelving unit. > > > > Does anyone have any experience with how well the old Tissue Tek plastic 6-drawer block cabinets stack - on top of each other? Can I safely do a stack of 5? And will they hold up well? > > > > Thanks in advance. > > Kim > > > > > > Kim Kolman > > > > Kimberly D. Kolman, HT, (ASCP) > > VA Eastern Kansas Health Care System > > Dwight D. Eisenhower VA Medical Center > > Histology - 115 > > 4101 S. 4th St. Trfwy. > > Leavenworth, KS 66048 > > 913-682-2000x52537 > > Fax: 913-758-4193 > > > > _______________________________________________ > From doug.porter <@t> caplab.org Tue Aug 26 17:09:26 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Tue Aug 26 17:05:41 2014 Subject: [Histonet] Re: Histonet Digest, Vol 129, Issue 34 In-Reply-To: <64C48E4F-75BE-48BC-AF49-5121D393AC90@rankinbiomed.com> References: <53fcbd5c.a34b3c0a.6c75.3162SMTPIN_ADDED_MISSING@mx.google.com> <64C48E4F-75BE-48BC-AF49-5121D393AC90@rankinbiomed.com> Message-ID: <006b01cfc17a$66bda830$3438f890$@caplab.org> We have been stacking them five high for many moons. They hold up quite well. Be mindful of your fire restrictions though. You have to maintain a certain clearance between the cabinets and the ceiling. Like 12 to 18 inches or so. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rob Rankin Sent: Tuesday, August 26, 2014 5:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 129, Issue 34 S > > > > ------------------------------ > > M > > > Dear Histo-netters: > > > > I'm looking to re-arrange my paraffin block storage; currently the plastic block cabinets are on a shelving unit. > > > > Does anyone have any experience with how well the old Tissue Tek plastic 6-drawer block cabinets stack - on top of each other? Can I safely do a stack of 5? And will they hold up well? > > > > Thanks in advance. > > Kim > > > > > > Kim Kolman > > > > Kimberly D. Kolman, HT, (ASCP) > > VA Eastern Kansas Health Care System > > Dwight D. Eisenhower VA Medical Center > > Histology - 115 > > 4101 S. 4th St. Trfwy. > > Leavenworth, KS 66048 > > 913-682-2000x52537 > > Fax: 913-758-4193 > > > > _______________________________________________ > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4745 / Virus Database: 4007/8105 - Release Date: 08/26/14 From ewillett <@t> seattlecca.org Tue Aug 26 21:00:54 2014 From: ewillett <@t> seattlecca.org (Willetts, Cheryl A) Date: Tue Aug 26 21:01:16 2014 Subject: [Histonet] Pneumocystis Controls Message-ID: <10041_1409104856_53FD3BD8_10041_189_1_9556ED7CAAF51D4792395EA1FD426C76799B0D84@EXDB02.seattlecca.org> Hi, We are looking for pneumocystis tissue blocks to use for controls, we are interested in trading blocks. Thank you, Cheryl Cheryl Willetts, HT (ASCP) Department of Pathology Seattle Cancer Care Alliance (206)288-2253 (206)288-1355 Pathology Lab **Confidentiality Notice** This communication may contain privileged and confidential information intended for the addressee. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of this information (including reliance thereon) is strictly prohibited. If you have received this communication in error, please notify us immediately and then destroy all copies of the communication. To view our complete Notice of Privacy Practices, visit our website at www.seattlecca.org. From TMcNemar <@t> lmhealth.org Wed Aug 27 05:00:02 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Aug 27 06:39:29 2014 Subject: [Histonet] RE: Looking for suggestions, ideas In-Reply-To: References: Message-ID: We did this a few years ago for our local coroner's office. They send us their tissues and we process them and cut the slides. We return the slides and blocks to them so there is nothing in our files. Are you returning the blocks with the slides? Pretty simple to do. I created a new specimen prefix in Meditech to differentiate them from our in-house cases and built a new charge procedure that has a zero charge for the professional component. The coroner does the dissection and places the tissues into the cassettes. The cassettes are a different color and their numbering system is unique so there's no problem identifying their cassettes. I assume that you will be placing the tissues into the cassettes on-site so I think that a different color cassette should solve your identification problem. You may want to think about also using a matching slide color to make it even simpler. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, August 26, 2014 4:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for suggestions, ideas Hi Folks, Here is our situation. In the not too distant future we will be receiving additional specimens from 3 dermatologists. What they need is to have these specimens processed, embedded, cut and H/E stained. Once we've got the slides coverslipped they are to be sent back the dermatologists. We are not doing the interpretation. What I'm looking for is an idea about getting these into our LIS (PowerPath) and designating them for return to this group. We should be able to capture the TC and need to account for the workload. I'm thinking we could prefix them differently at the time of accessioning and then create something in the LIS attached to that designation which only generates a TC. I'm wondering if anyone else does anything like this? I'm also interested in having this differing designation for efficient workflow. I don't want any of these making their way to our pathologists. I've thought of identification by color of block and a new prefix. Just not sure if/what others are doing, how difficult to implement for an LIS standpoint (separation of TC and PC) and any other considerations. Thanks, I know I've got all the superior minds out there. Your thoughts are greatly appreciated. Tom Jasper Thomas Jasper HT (ASCP) BAS AP/CP Supervisor Deaconess Hospital 600 Mary Street Evansville, IN 47747 Thomas.jasper@deaconess.com 812-450-2485 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From blayjorge <@t> gmail.com Wed Aug 27 08:30:12 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Wed Aug 27 08:30:22 2014 Subject: [Histonet] Ocular micrometers: recommendation of suppliers Message-ID: Dear Histonetters: I am about to request the purchase of two ocular micrometers, one for each of two compound microscopes. As there are many suppliers available, any recommendations on suppliers of ocular micrometers that offer them at a reasonable cost will be appreciated. Please, feel free to direct any such recommendations to blayjorge@gmail.com Thank you. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From juan.l.bassett.ctr <@t> mail.mil Wed Aug 27 10:12:30 2014 From: juan.l.bassett.ctr <@t> mail.mil (Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US)) Date: Wed Aug 27 10:12:42 2014 Subject: [Histonet] Slide remounting Message-ID: <5BEFD2C58946394F8E2A6EC3E1F86A9B1C81F2FE@umechpanz.easf.csd.disa.mil> Does anyone have a procedure that removes coverslips (w/crystallized permount present) ? The slides are 10+ years old. And is there a process to liquify the crystallized permount and refill w/o removing the coverslip? The mounted slides are 3 x 1's Juan l. Bassett HT ASCP Histotechnician Anatomical Div. National Museum of Health and Medicine 2500 Linden Lane Silver Spring, MD 20910 Office 301-319-3359 Lab 301-319-3328 Mobile 240-505-7247 Juan.l.bassett.ctr@mail.mil From Richard.Cartun <@t> hhchealth.org Wed Aug 27 10:46:10 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Aug 27 10:46:25 2014 Subject: [Histonet] protein A problem with Staph aureus immunostaining In-Reply-To: <740807818.88014.1409036707670.JavaMail.root@zstore.medstv.unimelb.edu.au> References: <20140825170928.54687AA048@mx1s.medstv.unimelb.edu.au>, <740807818.88014.1409036707670.JavaMail.root@zstore.medstv.unimelb.edu.au> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E355C8821@HHCEXCHMB03.hhcsystem.org> I don't have an answer for you, but I have observed this with IHC performed on human tissue where Staph is present (primarily skin biopsies). I have seen labeling of Staph organisms with many different types of antibodies (e.g., CD3, CD20, S-100, and more) and I have always attributed it to binding of immunoglobulin to "protein A" in the Staph. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Department of Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Jason Palmer [jpalmer@obi.edu.au] Sent: Tuesday, August 26, 2014 3:05 AM To: histonet@lists.utsouthwestern.edu Cc: Phong A Tran Subject: [Histonet] protein A problem with Staph aureus immunostaining Hi all, A question re. immunostaining of bacterial antigens. We are trying to stain cultured MRSA (Staph. aureus) with an antibody to PBP2a, which is the protein that confers antibiotic resistance to these bugs. So far we are finding non-specific binding of our antibodies to protein A is a real problem (or at least we assume that protein A is the problem). Primary is rabbit anti PBP2a and we follow this with a Cy3- conjugated anti rabbit IgG. We have tried normal serum blocking (10% rabbit) before the primary, as well as an Fc fragment of rabbit IgG as a more specific blocker of protein A before the primary, but so far without much success; we see labe ling with our primary, with an isotype control and also with diluent alone. So it would seem that both our primary and secondary is binding to protein A non-specifically. Next things to try are increasing the concentration of the Fc fragment blocking (was about 100ug/ml the first time) and also to try an Fab fragment as a secondary, rather than the whole IgG we currently use. Does anybody have any other suggestions as to what we might try to get on top of this problem? I have only ever immunostained mammalian cells and tissues before, so this is my first time with bacteria. The dyes I'm using (Cy3 and DAPI) photobleach very easily for some reason with these bugs compared with your average mammalian cells? Thanks for any suggestions, Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jpalmer@obi.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From baderbo <@t> gmail.com Wed Aug 27 11:09:41 2014 From: baderbo <@t> gmail.com (Bader Siddiki) Date: Wed Aug 27 11:09:45 2014 Subject: [Histonet] Slide remounting In-Reply-To: <5BEFD2C58946394F8E2A6EC3E1F86A9B1C81F2FE@umechpanz.easf.csd.disa.mil> References: <5BEFD2C58946394F8E2A6EC3E1F86A9B1C81F2FE@umechpanz.easf.csd.disa.mil> Message-ID: Hello Soak slides in xylene or equivalent solvent for sometimes till the coverslip is loose, carefully remove the coverslip; you can remount the slides with Permount. You can dissolve the Permount in organic solvents, but the glue will be dissolved and you have to remount the slide. Bader On Wed, Aug 27, 2014 at 8:12 AM, Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US) wrote: > > Does anyone have a procedure that removes coverslips (w/crystallized > permount present) ? The slides are 10+ years old. And is there a process > to liquify the crystallized permount and refill w/o removing the > coverslip? The mounted slides are 3 x 1's > > Juan l. Bassett HT ASCP > Histotechnician Anatomical Div. > National Museum of Health and Medicine > 2500 Linden Lane > Silver Spring, MD 20910 > Office 301-319-3359 > Lab 301-319-3328 > Mobile 240-505-7247 > > Juan.l.bassett.ctr@mail.mil > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Gr??en / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten ?????? muito agradecido/a(s) Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience Corp. (IBSC) Website: www.ImmunoBioScience.Com *E-mail: BaderBo@GMail.com* Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 Cell (Mobile) phone: + 1 425 314 0199 Address: PO Box 218 Mukilteo, WA 98275-0218 USA Marketing person: Anita E-Mail: AnitaIBSC@AOL.com From JWatson <@t> gnf.org Wed Aug 27 11:30:04 2014 From: JWatson <@t> gnf.org (James Watson) Date: Wed Aug 27 11:30:08 2014 Subject: [Histonet] RE: Slide remounting In-Reply-To: <5BEFD2C58946394F8E2A6EC3E1F86A9B1C81F2FE@umechpanz.easf.csd.disa.mil> References: <5BEFD2C58946394F8E2A6EC3E1F86A9B1C81F2FE@umechpanz.easf.csd.disa.mil> Message-ID: With slides this old you can usually spray the back of the slide with freeze spray and the coverslip will pop off. Then soak in xylene and once any remaining permount is gone, re-coverslip. We did this routinely at AFIP, in the old days. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US) Sent: Wednesday, August 27, 2014 8:13 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide remounting Importance: High Does anyone have a procedure that removes coverslips (w/crystallized permount present) ? The slides are 10+ years old. And is there a process to liquify the crystallized permount and refill w/o removing the coverslip? The mounted slides are 3 x 1's Juan l. Bassett HT ASCP Histotechnician Anatomical Div. National Museum of Health and Medicine 2500 Linden Lane Silver Spring, MD 20910 Office 301-319-3359 Lab 301-319-3328 Mobile 240-505-7247 Juan.l.bassett.ctr@mail.mil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From blayjorge <@t> gmail.com Wed Aug 27 16:08:07 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Wed Aug 27 16:08:16 2014 Subject: [Histonet] Paper of possible (insect) histology interest, link inside Message-ID: http://blaypublishers.com/2014/07/18/leb-21-2014/ If interested, click on 4. LEB 2(1) Ozyurt et al. Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From nalesnikma <@t> upmc.edu Wed Aug 27 21:36:12 2014 From: nalesnikma <@t> upmc.edu (Nalesnik, Michael) Date: Wed Aug 27 21:36:19 2014 Subject: [Histonet] Job availability Message-ID: <17118600C437C0458A78E63C8E50F512011299FF@MSXMBXNSPRD03.acct.upmchs.net> Hello Histonetters! I have subscribed to this message board for awhile and would first like to thank you for all of the helpful suggestions I have picked up just by reading the posts. I am writing because we have an opening for a Histology Supervisor in our laboratory. This is at the University of Pittsburgh Medical Center (UPMC) in Pittsburgh, Pennsylvania. It is a large and busy lab that services several hospitals in our system. Our hospital was ranked #12 nationally and #1 in Pennsylvania for 2014-5 by US News and World Report. We have recently undergone CAP inspection with no deficiencies cited and are seeking to strengthen our lab by staying current (or ahead of the curve) while keeping 40 or so pathologists happy at the same time. Given the size of the lab we have a laboratory manager who is currently doing yeoman's work in performing the work of both positions for now. We are looking for a knowledgeable person who is independent and can work with and manage people. We also need a person who is open to innovations that may arise as a result of the forces that are impacting the way that pathology is practiced. I think this would be a challenging and satisfying position for the right person. Pittsburgh has frequently been named as one of the best cities to live in the US, most recently by the Economist (excluding Honolulu!) and as a transplant from New Jersey who has lived here since 1977, I can vouch for the fact that it is a very enjoyable place to live regardless of whether you are young or old. If you think this might interest you, or if you would like more information, feel free to contact either me or our lab manager Rachel Wall (wallrd@upmc.edu) who is more familiar with the specifics of the day-to-day operations of the lab. Look forward to hearing from you, and thanks! Michael A. Nalesnik, M.D. Professor of Pathology Medical Director, Histology and Immunocytochemistry Laboratories Division of Transplantation and Hepatic Pathology nalesnikma@upmc.edu From CDavis <@t> che-east.org Thu Aug 28 11:37:46 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Thu Aug 28 11:38:05 2014 Subject: [Histonet] p16 from XT to Ultra Message-ID: Hi histoland, You guys were wonderful helping me with the MUM-1 on the XT a few months ago, I am hoping you can help me now. I am trying to optimize the p16 on the Ultra and having a little trouble. It is B-E-A-utiful on the XT but does not want to play nice on the Ultra. Any tips...Anyone? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From craigak12 <@t> gmail.com Thu Aug 28 13:21:03 2014 From: craigak12 <@t> gmail.com (Jb) Date: Thu Aug 28 13:21:22 2014 Subject: [Histonet] Xylene/Formalin monitoring: Message-ID: <941790AE-0FBD-4990-875E-A942FC9A26B6@gmail.com> Does anyone know the CAP standard for monitoring xylene/formalin? Is it each employee 8 hrs & 4 hrs for each? Thank you. Sent from my iPhone From Lacie.Algeo <@t> providence.org Thu Aug 28 13:24:11 2014 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Thu Aug 28 13:25:03 2014 Subject: [Histonet] p16 from XT to Ultra Message-ID: <24C4B3C167E5694887AB594C7602CE3A0D0635@WN35104.or.providence.org> Hi Cassandra, We started with the original recommended protocol from Ventana for the Ultra and tested at one incubation above and below. Are you using iView or Ultra View detection? We use the Ultra View. Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, August 28, 2014 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 129, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Paper of possible (insect) histology interest, link inside (Jorge A. Santiago-Blay) 2. Job availability (Nalesnik, Michael) 3. p16 from XT to Ultra (Davis, Cassie) ---------------------------------------------------------------------- Message: 1 Date: Wed, 27 Aug 2014 17:08:07 -0400 From: "Jorge A. Santiago-Blay" Subject: [Histonet] Paper of possible (insect) histology interest, link inside To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 http://blaypublishers.com/2014/07/18/leb-21-2014/ If interested, click on 4. LEB 2(1) Ozyurt et al. Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html ------------------------------ Message: 2 Date: Thu, 28 Aug 2014 02:36:12 +0000 From: "Nalesnik, Michael" Subject: [Histonet] Job availability To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <17118600C437C0458A78E63C8E50F512011299FF@MSXMBXNSPRD03.acct.upmchs.net> Content-Type: text/plain; charset="us-ascii" Hello Histonetters! I have subscribed to this message board for awhile and would first like to thank you for all of the helpful suggestions I have picked up just by reading the posts. I am writing because we have an opening for a Histology Supervisor in our laboratory. This is at the University of Pittsburgh Medical Center (UPMC) in Pittsburgh, Pennsylvania. It is a large and busy lab that services several hospitals in our system. Our hospital was ranked #12 nationally and #1 in Pennsylvania for 2014-5 by US News and World Report. We have recently undergone CAP inspection with no deficiencies cited and are seeking to strengthen our lab by staying current (or ahead of the curve) while keeping 40 or so pathologists happy at the same time. Given the size of the lab we have a laboratory manager who is currently doing yeoman's work in performing the work of both positions for now. We are looking for a knowledgeable person who is independent and can work with and manage people. We also need a person who is open to innovations that may arise as a result of the forces that are impacting the way that pathology is practiced. I think this would be a challenging and satisfying position for the right person. Pittsburgh has frequently been named as one of the best cities to live in the US, most recently by the Economist (excluding Honolulu!) and as a transplant from New Jersey who has lived here since 1977, I can vouch for the fact that it is a very enjoyable place to live regardless of whether you are young or old. If you think this might interest you, or if you would like more information, feel free to contact either me or our lab manager Rachel Wall (wallrd@upmc.edu) who is more familiar with the specifics of the day-to-day operations of the lab. Look forward to hearing from you, and thanks! Michael A. Nalesnik, M.D. Professor of Pathology Medical Director, Histology and Immunocytochemistry Laboratories Division of Transplantation and Hepatic Pathology nalesnikma@upmc.edu ------------------------------ Message: 3 Date: Thu, 28 Aug 2014 12:37:46 -0400 From: "Davis, Cassie" Subject: [Histonet] p16 from XT to Ultra To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi histoland, You guys were wonderful helping me with the MUM-1 on the XT a few months ago, I am hoping you can help me now. I am trying to optimize the p16 on the Ultra and having a little trouble. It is B-E-A-utiful on the XT but does not want to play nice on the Ultra. Any tips...Anyone? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 129, Issue 36 ***************************************** ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From blayjorge <@t> gmail.com Thu Aug 28 13:29:13 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Thu Aug 28 13:29:18 2014 Subject: [Histonet] Checklist light microscope repair Message-ID: Dear Histonetters: Does anyone have (or know of) a a checklist for the repair of light microscopes? I have been surfing the web the closest I see is something like this: http://www.flinnsci.com/teacher-resources/biology/microscope-maintenance-tips.aspx I think a checklist would be a nice thing to have handy. If you can help, please feel free to email me directly. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From plucas <@t> biopath.org Thu Aug 28 16:02:25 2014 From: plucas <@t> biopath.org (Paula Lucas) Date: Thu Aug 28 15:56:43 2014 Subject: [Histonet] Grossing/PA needed, orange county ca Message-ID: <4F39552CCC3E41B290DAD09439344393@biopath.local> We need assistance with our volume. Please call if interested and if you have any questions. Please ask for Russell Blayney. Thank you, Paula Lucas Lab Manager Biopath Medical Group 714-433-1330 From Lacie.Algeo <@t> providence.org Thu Aug 28 17:20:30 2014 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Thu Aug 28 17:20:48 2014 Subject: [Histonet] processing biopsies on VIP Message-ID: <24C4B3C167E5694887AB594C7602CE3A0D0765@WN35104.or.providence.org> Hi there, Does anyone use P/V on their biopsy runs on the Tissue-Tek VIP 5? We're having really dry biopsies, and I think this could be why.... Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From ibernard <@t> uab.edu Thu Aug 28 21:52:17 2014 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Thu Aug 28 21:52:25 2014 Subject: [Histonet] RE: processing biopsies on VIP In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A0D0765@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A0D0765@WN35104.or.providence.org> Message-ID: Check your protocol. Heat or temp is a factor and length of time in alcohol. We have P/V for the 10 % NBF and paraffin infiltration. IB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Thursday, August 28, 2014 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing biopsies on VIP Hi there, Does anyone use P/V on their biopsy runs on the Tissue-Tek VIP 5? We're having really dry biopsies, and I think this could be why.... Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Fri Aug 29 05:57:13 2014 From: j.rowaihi <@t> alborglaboratories.com (j.rowaihi) Date: Fri Aug 29 05:57:34 2014 Subject: =?UTF-8?Q?=D8=B1=D8=AF:_[Histonet]_Xylene/Formalin_monitoring:?= Message-ID: Hi CAP checklist has enough details about monitoring the Formaldehyde and Xylene exposure and vapors.? However, use formaldehyde & Xylene badges in the working areas for 8 hours and send it for measuring the exposure percentage. From the result you can decide if you're in the green or warning or hazardous limits, refer to the badges pamphlet. Regards Jamal Rowaihi Anatomic Pathology Supervisor Al Borg Medical Laboratories CAP accredited.? *Sent from my cell phoner* -------- ??????? ??????? -------- ??: Jb ???????: ????/??/?? ??:?? ? (GMT+04:00) ???: histonet@lists.utsouthwestern.edu ???????: [Histonet] Xylene/Formalin monitoring: Does anyone know the CAP standard for monitoring xylene/formalin? Is it each employee 8 hrs & 4 hrs for each? Thank you. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi <@t> alborglaboratories.com Fri Aug 29 06:17:43 2014 From: j.rowaihi <@t> alborglaboratories.com (j.rowaihi) Date: Fri Aug 29 06:18:02 2014 Subject: =?UTF-8?Q?=D8=B1=D8=AF:_[Histonet]_RE:_pr?= =?UTF-8?Q?ocessing_biopsies_on_VIP?= Message-ID: Hello colleagues I am using this machine since long time.? P/V mostly is not the cause of tissue drying,? Check the wax baths temperature if it's not over 2 degrees from the paraffin wax melting point.? Make sure the temperature in Xylene steps is not over 37 C degree. Check the purity of the ethanol, specially the absolute ethanol.? Regards Jamal Rowaihi Anatomic Pathology Supervisor Al Borg Medical Laboratories *Sent from my cell phoner* -------- ??????? ??????? -------- ??: Ian R Bernard ???????: ????/??/?? ?:?? ? (GMT+04:00) ???: "Algeo, Lacie A" ,histonet@lists.utsouthwestern.edu ???????: [Histonet] RE: processing biopsies on VIP Check your protocol.? Heat? or temp is a factor and length of time in alcohol. We have P/V for the 10 % NBF and paraffin infiltration. IB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Thursday, August 28, 2014 4:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing biopsies on VIP Hi there, Does anyone use P/V on their biopsy runs on the Tissue-Tek VIP 5?? We're having really dry biopsies, and I think this could be why.... Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law.? If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message.? If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Aug 29 09:01:11 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Aug 29 09:01:16 2014 Subject: [Histonet] Funny, hope link works Message-ID: http://tissuepathology.com/2014/08/28/bye-bye-88305-sung-to-american-pie/#axzz3Bn0mloT9 Joelle Weaver MAOM, HTL (ASCP) QIHC From victor_tobias <@t> comcast.net Fri Aug 29 09:33:26 2014 From: victor_tobias <@t> comcast.net (victor_tobias@comcast.net) Date: Fri Aug 29 09:33:43 2014 Subject: [Histonet] Funny, hope link works In-Reply-To: References: Message-ID: <708727401.2191585.1409322806570.JavaMail.root@comcast.net> Thanks Joelle, I love it. ? Victor ----- Original Message ----- From: "Joelle Weaver" To: histonet@lists.utsouthwestern.edu Sent: Friday, August 29, 2014 7:01:11 AM Subject: [Histonet] Funny, hope link works ?http://tissuepathology.com/2014/08/28/bye-bye-88305-sung-to-american-pie/#axzz3Bn0mloT9 Joelle Weaver MAOM, HTL (ASCP) QIHC ?? ? ? ? ?? ????????????????? ???????? ? ???????????????? ?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cheastys <@t> svm.vetmed.wisc.edu Fri Aug 29 11:58:03 2014 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Fri Aug 29 11:58:09 2014 Subject: [Histonet] Preventative Maintenance Fees Gone Wild Message-ID: <8bfa3a806cbb7d47b8bd122cf28e7bc2@svm.vetmed.wisc.edu> Does anyone else think that $8,266 sounds a bit high for a PM on a Lab Vision Immunostainer? Does that price include a Happy Ending? From tawnia <@t> ampianstaffing.com Fri Aug 29 12:07:36 2014 From: tawnia <@t> ampianstaffing.com (Tawnia Lindsay) Date: Fri Aug 29 12:07:41 2014 Subject: [Histonet] Grossing need Message-ID: I have a grossing need in Arizona, for a temp to perm position in a great lab. I am looking for someone who has a few years experience and wants to work in a busy lab with a great team. Please let me know if you are interested. Thanks, Tawnia Lindsay Ampian Staffing, Inc. Please note new address 126 W. Sego Lily, Suite 110 Sandy, Utah 84070 O: 877-229-6996 Ext 2009 F: 801-253-6127 E: tawnia@ampianstaffing.com Website: www.ampianstaffing.com From tgenade <@t> gmail.com Fri Aug 29 12:12:22 2014 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Aug 29 12:12:28 2014 Subject: [Histonet] detached retinas Message-ID: Hello, I'm having a problem with detached retinas. I fix my fish, whole, in 4% PFA for 24 hours. The belly is slit to allow better penetration of the PFA. The entire fish is about 4 cm long and about 6 mm wide. From the 4% PFA I proceed to decalsify the fish in 14% EDTA (pH 7.6). This goes on for 3 days. From there the tissues is passaged through 70% to 95% to 100% ethanol and then cleared in methylbenzoate before being wax impregnated. My current hypothesis is that the thick eye membrane is impeding the penetration of the PFA. As consequence, when I passage into the EDTA and alcohol I get rapid dehydration of the tissue and the change in pressure in the eye causes the detached retinas. How feasible is this hypothesis and what others can you come up with? I am currently testing this hypothesis. I have fixed 2 fish in PFA but I have punctured the eyes of one of the fish to improve PFA penetration. The sections I have cut of the first specimen show no issues with soft tissue fixation. My only problem is that the retina has detached formed several waves of tissue in the eye. The sections look good except for the eye issues. I never had this issue with Bouin's. Thanks -- Tyrone Genade P.S. Incidentally, I am now using Paraplast Plus cat number 19216 ( http://www.emsdiasum.com/microscopy/products/histology/embedding.aspx ) and it is a dream to work with compared to what I was using. It is soft and cuts beautifully, the ribbons look good and impregnation is great (even by manual processing). Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From liz <@t> premierlab.com Fri Aug 29 12:26:16 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Aug 29 12:26:22 2014 Subject: [Histonet] detached retinas In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECD65B@SBS2K8.premierlab.local> Tyrone I'm not sure if this will be helpful or not but we do a lot of work with mouse and rat eyes. We have seen that with both 10% NBF and 4% PFA there will be retinal detachment regardless of how you process the samples. Fixation in modified Davidsons or Davidsons in our experience is the only way that we are able to maintain good morphology of the retina without it detaching. I'm not sure how that will affect the fixation of the entire fish, since I do not have experience in processing fish samples. We have also found that you cannot process the eyes whole if you want any decent retina morphology, for us since we are just dealing with eye samples and not an entire fish, we will trim the edge off of both the rat or mouse eyes that we are running retinal studies on. I hope this helps. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Friday, August 29, 2014 11:12 AM To: histonet Subject: [Histonet] detached retinas Hello, I'm having a problem with detached retinas. I fix my fish, whole, in 4% PFA for 24 hours. The belly is slit to allow better penetration of the PFA. The entire fish is about 4 cm long and about 6 mm wide. From the 4% PFA I proceed to decalsify the fish in 14% EDTA (pH 7.6). This goes on for 3 days. From there the tissues is passaged through 70% to 95% to 100% ethanol and then cleared in methylbenzoate before being wax impregnated. My current hypothesis is that the thick eye membrane is impeding the penetration of the PFA. As consequence, when I passage into the EDTA and alcohol I get rapid dehydration of the tissue and the change in pressure in the eye causes the detached retinas. How feasible is this hypothesis and what others can you come up with? I am currently testing this hypothesis. I have fixed 2 fish in PFA but I have punctured the eyes of one of the fish to improve PFA penetration. The sections I have cut of the first specimen show no issues with soft tissue fixation. My only problem is that the retina has detached formed several waves of tissue in the eye. The sections look good except for the eye issues. I never had this issue with Bouin's. Thanks -- Tyrone Genade P.S. Incidentally, I am now using Paraplast Plus cat number 19216 ( http://www.emsdiasum.com/microscopy/products/histology/embedding.aspx ) and it is a dream to work with compared to what I was using. It is soft and cuts beautifully, the ribbons look good and impregnation is great (even by manual processing). Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From craigak12 <@t> gmail.com Fri Aug 29 12:29:15 2014 From: craigak12 <@t> gmail.com (Jb) Date: Fri Aug 29 12:29:21 2014 Subject: [Histonet] IHC Validation: Message-ID: <6E017940-E2FA-4A97-B423-D8904C32C29B@gmail.com> How do most people validate IHC? I want to create a tissue microarray. I wanted to use an average of 6-8 positive tissues. Is it necessary to validate using negative tissues when there is always an internal negative control in all tissue sections. Now with new polymer detection systems there is not background, etc. Is IHC validation ultimately up to the discretion of the laboratory director? Please advise. Thx- Sent from my iPhone From Steven.Swartwood <@t> cshs.org Fri Aug 29 12:34:22 2014 From: Steven.Swartwood <@t> cshs.org (Swartwood, Steven J) Date: Fri Aug 29 12:34:28 2014 Subject: [Histonet] ZDHHC11 Message-ID: <959202AC61AEF942968646EC66E2BE3E13A51857@ESPWMSGMBX07.CSMC.EDU> Hello Histonetters, This is all pertaining to staining human tissues, I work in a research core lab and was wondering if anyone had IHC-P (FFPE) experience in staining for ZDHHC11? From the data our computational team has pulled 70% of primary prostate ca is positive and 90% of metastatic prostate ca is positive. There's not much information out there for IHC-P staining with this antibody. The researcher that wants this staining done bought this from Thermo Scientific and they don't have much information either... other than a picture of lung adenocarcinoma that about 4 other companies also show from the same antibody. One company advised using pepsin digestion along with citrate retrieval and another said tris-EDTA. I figure to try out multiple retrievals (as usual with new antibodies), but has anyone ever used this antibody? I was trying to find some good control tissue as well (besides the cancer tissues) and all I can find is normal ovary so far. We have a Discovery Ultra here and I've tried a titer with a few different high grade primary prostate cancer tissues with different HIER and was unsuccessful, but I've yet to try the enzymatic approach. Any help would be tremendously appreciated, Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartwood@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. From joelleweaver <@t> hotmail.com Fri Aug 29 16:09:13 2014 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Aug 29 16:09:23 2014 Subject: [Histonet] IHC Validation: In-Reply-To: <6E017940-E2FA-4A97-B423-D8904C32C29B@gmail.com> References: <6E017940-E2FA-4A97-B423-D8904C32C29B@gmail.com> Message-ID: Yes, ultimately up to lab director/medical director/pathologist as to determination of specificity, selectivity, and if you have enough examples, and the staining reactivity conforms to the "intended clinical use" during their assessment and hopefully approval of your protocol. I have used some TMAs with success, especially if you make with your own in-house processed tissues. I try to strongly favor using several expression levels of normal and diseased tissue whenever possible that reflect what it will be used for in the patient test tissues. If single sections, I try use both expected or known negative and positive tissue both normal and diseased , when practical for the first validation set when I get past optimization. For small adjustments I may need only a few more confirming positives- up to MD in my situation. I also have polymer detection, but I still like some negatives for me. Some people may not feel this is necessary, and the pathologist may not need the negatives ( using internal controls), but this helps me, so I do it to feel more confident in my results as I present the slides for review. I don't see why you couldn't use internal negatives, if you clarify what tissue element acts as the internal negative in the tissue type in the validation summary and SOP. Basically, for amount or # to stain, I follow the CAP guidelines ( newer ones), for well characterized. For markers with specific guidelines for validation and correlation, I follow the CAP guidelines exactly. Setting up the process/SOP s, I used the CLSI guidebook on validation of IHC assays. Both resources (CAP & CLSI) have been very helpful for me. That is what has been working for me, I hope this helps. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: craigak12@gmail.com > Date: Fri, 29 Aug 2014 10:29:15 -0700 > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] IHC Validation: > > How do most people validate IHC? I want to create a tissue microarray. I wanted to use an average of 6-8 positive tissues. > > Is it necessary to validate using negative tissues when there is always an internal negative control in all tissue sections. Now with new polymer detection systems there is not background, etc. > > Is IHC validation ultimately up to the discretion of the laboratory director? > > Please advise. Thx- > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andre.charbonneau2 <@t> mail.mcgill.ca Fri Aug 29 18:10:37 2014 From: andre.charbonneau2 <@t> mail.mcgill.ca (=?iso-8859-1?Q?Andr=E9_Charbonneau?=) Date: Fri Aug 29 18:10:41 2014 Subject: [Histonet] Sirius Red (Normal Light) Yellow Colour Degradation Message-ID: <232D1176-4C09-4962-9733-51AD562D732E@mail.mcgill.ca> Hello to all, This is a quick comment I would like to mention to people completing the Sirius Red staining. If you don't seem to have the desired yellow cytoplasm ( I have seen in axolotl red blood cells) then the issue might be because you Sirius Red liquid is not longer effective. I know this might seem trivial but I was reading some previous posts that mention that the solution is good for up to 12 years. Personally, I have stained several hundred slides with the same solution of ~ 250ml of Sirius Red and gradually, the yellow found in the muscle and in the cytoplasm in normal light started becoming less vibrant. The solution can probably be kept for 12 years but it has limited amounts of usage. Once I re-made my solution, the yellow cytoplasm and muscles were vibrantly yellow again. This is different from other troubleshooting comments that were posted earlier which said to keep the slides in a shorter amount of time in the alcohols during the dehydration. Simply changing the Sirius Red would be the way to go. Both old Sirius Red and new were compared at the same time using same alcohols and acetic acids, thus removing the probability that other factors affected the colouring. Sirius Red 0.1% in Sat Sol. of Picric Acid: Direct Red 80 0.1g Sat Sol. Picric Acid 100ml Andr? Charbonneau M.Sc. Candidate Faculty of Dentistry McGill University From b427297 <@t> aol.com Fri Aug 29 18:27:37 2014 From: b427297 <@t> aol.com (b427297@aol.com) Date: Fri Aug 29 18:27:47 2014 Subject: [Histonet] Preventative Maintenance Fees Gone Wild In-Reply-To: <8bfa3a806cbb7d47b8bd122cf28e7bc2@svm.vetmed.wisc.edu> References: <8bfa3a806cbb7d47b8bd122cf28e7bc2@svm.vetmed.wisc.edu> Message-ID: <7371EE12-9B72-45DD-AF34-DFAEC54EFAE2@aol.com> Pm or a service contract? Sent from my iPhone > On Aug 29, 2014, at 11:58 AM, "Sandra Cheasty" wrote: > > Does anyone else think that $8,266 sounds a bit high for a PM on a Lab Vision Immunostainer? Does that price include a Happy Ending? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Steven.Weston <@t> utas.edu.au Sat Aug 30 03:10:34 2014 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Sat Aug 30 03:10:52 2014 Subject: [Histonet] Re cd4 1F6 clone Message-ID: <7B808A2E6BDDBD4EA4FA6395FF7BED2BC38BE863@MBXSBYN2.utas.ad.internal> Hi Histonetters Has anyone else had difficulty getting this clone to work? cd4 1F6 from leica microsystems (formerly novocastra). I've tried just about every trick in my book and still no staining. Particularly i made sure i did the peroxidase block prior to HIER (i even tried using it after the primary) and have tried low, high and middle pH buffers with antibody concentrations from 1/10-1/40 for 60 and 90 mins and use envision plus detection with DAB plus. I've had two batches of this over the last ten years and neither has worked. Anyone else with the same experience ? regards steve weston lab manager Breathe-Well CRE UTAS-SOM From neerajbioxford <@t> gmail.com Sat Aug 30 05:14:09 2014 From: neerajbioxford <@t> gmail.com (Neeraj Singh) Date: Sat Aug 30 05:14:25 2014 Subject: [Histonet] Re cd4 1F6 clone In-Reply-To: <7B808A2E6BDDBD4EA4FA6395FF7BED2BC38BE863@MBXSBYN2.utas.ad.internal> References: <7B808A2E6BDDBD4EA4FA6395FF7BED2BC38BE863@MBXSBYN2.utas.ad.internal> Message-ID: <1663FC3E-E152-411F-B553-83D3D426ACEE@gmail.com> Try with no H2O2 at all for this clone. it will hill you. Thanks Neeraj On Aug 30, 2014, at 1:40 PM, Steven Weston wrote: > Hi Histonetters > Has anyone else had difficulty getting this clone to work? cd4 1F6 from leica microsystems (formerly novocastra). > I've tried just about every trick in my book and still no staining. > Particularly i made sure i did the peroxidase block prior to HIER (i even tried using it after the primary) and have tried low, high and middle pH buffers with antibody concentrations from 1/10-1/40 for 60 and 90 mins and use envision plus detection with DAB plus. I've had two batches of this over the last ten years and neither has worked. > Anyone else with the same experience ? > regards > steve weston > lab manager > Breathe-Well CRE > UTAS-SOM From tgenade <@t> gmail.com Sat Aug 30 12:06:14 2014 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Sat Aug 30 12:06:24 2014 Subject: Subject: RE: [Histonet] detached retinas Message-ID: Thanks to those who volunteered their advice and opinions. One solution was offered by Elizabeth that I will be trying in modified form. One comment was that the retinas will always detach in an aqueous fixative but retina quality was very good with Bouin's. Any thoughts on why this might be? As an aside, a long while back some advice was dispensed regarding the use of tea-bags for small histological samples. This works fantastically! -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From LSebree <@t> uwhealth.org Sat Aug 30 18:45:33 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Sat Aug 30 18:45:39 2014 Subject: [Histonet] IHC Validation: In-Reply-To: <6E017940-E2FA-4A97-B423-D8904C32C29B@gmail.com> References: <6E017940-E2FA-4A97-B423-D8904C32C29B@gmail.com> Message-ID: <77DD817201982748BC67D7960F2F76AF0D6274@UWHC-MBX12.uwhis.hosp.wisc.edu> I think your last question says it all; it ultimately is up to the director although she/he better be able to explain to a CAP inspector why it was done the way it was done. We generally follow the CAP guidelines with 10 - 20 +/10-20 - cases. We've also used the internal negative elements as being negative as expected in our validation documentation. In some cases, when + cases are rare we've used less than the suggested number just as CAP says is acceptable but we've usually been able to come up with enough. TMAs are a great way to go if you have them or can make them; cuts down on labor/reagent cost. Linda A. Sebree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Jb [craigak12@gmail.com] Sent: Friday, August 29, 2014 12:29 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation: How do most people validate IHC? I want to create a tissue microarray. I wanted to use an average of 6-8 positive tissues. Is it necessary to validate using negative tissues when there is always an internal negative control in all tissue sections. Now with new polymer detection systems there is not background, etc. Is IHC validation ultimately up to the discretion of the laboratory director? Please advise. Thx- Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Sat Aug 30 18:47:48 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Sat Aug 30 18:47:52 2014 Subject: [Histonet] IHC Validation: In-Reply-To: References: <6E017940-E2FA-4A97-B423-D8904C32C29B@gmail.com>, Message-ID: <77DD817201982748BC67D7960F2F76AF0D6283@UWHC-MBX12.uwhis.hosp.wisc.edu> Well said Joelle; we do pretty much the same. Linda A. Sebree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Joelle Weaver [joelleweaver@hotmail.com] Sent: Friday, August 29, 2014 4:09 PM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC Validation: Yes, ultimately up to lab director/medical director/pathologist as to determination of specificity, selectivity, and if you have enough examples, and the staining reactivity conforms to the "intended clinical use" during their assessment and hopefully approval of your protocol. I have used some TMAs with success, especially if you make with your own in-house processed tissues. I try to strongly favor using several expression levels of normal and diseased tissue whenever possible that reflect what it will be used for in the patient test tissues. If single sections, I try use both expected or known negative and positive tissue both normal and diseased , when practical for the first validation set when I get past optimization. For small adjustments I may need only a few more confirming positives- up to MD in my situation. I also have polymer detection, but I still like some negatives for me. Some people may not feel this is necessary, and the pathologist may not need the negatives ( using internal controls), but this helps me, so I do it to feel more confident in my results as I present the slides for review. I don't see why you couldn't use internal negatives, if you clarify what tissue element acts as the internal negative in the tissue type in the validation summary and SOP. Basically, for amount or # to stain, I follow the CAP guidelines ( newer ones), for well characterized. For markers with specific guidelines for validation and correlation, I follow the CAP guidelines exactly. Setting up the process/SOP s, I used the CLSI guidebook on validation of IHC assays. Both resources (CAP & CLSI) have been very helpful for me. That is what has been working for me, I hope this helps. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: craigak12@gmail.com > Date: Fri, 29 Aug 2014 10:29:15 -0700 > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] IHC Validation: > > How do most people validate IHC? I want to create a tissue microarray. I wanted to use an average of 6-8 positive tissues. > > Is it necessary to validate using negative tissues when there is always an internal negative control in all tissue sections. Now with new polymer detection systems there is not background, etc. > > Is IHC validation ultimately up to the discretion of the laboratory director? > > Please advise. Thx- > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Steven.Weston <@t> utas.edu.au Sun Aug 31 18:49:20 2014 From: Steven.Weston <@t> utas.edu.au (Steven Weston) Date: Sun Aug 31 18:49:32 2014 Subject: [Histonet] CD4 1F6 clone Message-ID: I also tried it without H2O2 with no result either. Anyone else had these problems with this clone ??? Interestingly in the past I used the duo pack of CD4/8 that had the 1F6 for cd4 and the 4B11 clone of cd8 and they both worked. May have to try the 4b12 clone for cd4. regards Steve Weston Message: 10 Date: Sat, 30 Aug 2014 15:44:09 +0530 From: Neeraj Singh > Subject: Re: [Histonet] Re cd4 1F6 clone To: Steven Weston > Cc: "histonet@lists.utsouthwestern.edu" > Message-ID: <1663FC3E-E152-411F-B553-83D3D426ACEE@gmail.com> Content-Type: text/plain; charset=us-ascii Try with no H2O2 at all for this clone. it will hill you. Thanks Neeraj On Aug 30, 2014, at 1:40 PM, Steven Weston > wrote: Hi Histonetters Has anyone else had difficulty getting this clone to work? cd4 1F6 from leica microsystems (formerly novocastra). I've tried just about every trick in my book and still no staining. Particularly i made sure i did the peroxidase block prior to HIER (i even tried using it after the primary) and have tried low, high and middle pH buffers with antibody concentrations from 1/10-1/40 for 60 and 90 mins and use envision plus detection with DAB plus. I've had two batches of this over the last ten years and neither has worked. Anyone else with the same experience ? regards steve weston lab manager Breathe-Well CRE UTAS-SOM Steve Weston Lab Manager Breathe-Well Centre of Research Excellence for Chronic Respiratory Disease. UTAS-SOM 0408990859 62264871