[Histonet] RE: proceesing of GI Bxs

[Karen Lahti]

We have a medium GI lab using the old, old VIP's.  We have been very successful with two processing schedules, long and short.  We found that we needed to add a bit of heat to the last xylene so when the tissue hit the first paraffin, they would start to infiltrate.  The cold cassettes solidified the paraffin and the time spent was melting the paraffin not infiltrating the biopsy.  For the longer run, we add 10%NBF and a bit longer in each solution.

Short:
1.  70% reagent alcohol - 15 min, 40C, P/V
2.  85% reagent alcohol - 10 min, 40C, P/V
3.  85% reagent alcohol - 15 min, 40C, P/V
4.  100% reagent alcohol - 10 min, 40C, P/V
5.  100% reagent alcohol - 10 min, 40C, P/V
6.  100% reagent alcohol - 10 min, 40C, P/V
7.  Xylene - 20 min, 40C, P/V
8.  Xylene - 20 min, 50C, P/V
9.  Paraffin - 10 min, 58C, P/V
10.  Paraffin - 30 min, 58C, P/V
11.  Paraffin - 45 min, 58C, P/V

 Karen Lahti, HT (ASCP), QIHC
Arizona Digestive Health
602-687-7468


On Apr 3, 2014, at 9:33 AM, "Arguello, Daniel" <arguellod <@t> ihn.org> wrote:

> I'd appreciate a copy of that processing schedule for GI bxs as well.
> Thank you in advance
> 
> Sincerely, 
> 
> Daniel Argüello, BS, CT (ASCP)CM
> Anatomic Pathology Services Coordinator
> Inspira Medical Center Woodbury
> 509 North Broad Street
> Woodbury, NJ 08096
> 856-853-2030 Ext 2808
> 856-853-2183 Fax
> arguellod <@t> ihn.org
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vicki McKaughan
> Sent: Thursday, April 03, 2014 12:32 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] proceesing of GI Bxs
> 
> Could someone please send me your processing schedule for GI bxs.  We are using a Sakura VIP5 and are having problems with over drying and occasionally with infiltration of paraffin.  Thanks _______________________________________________
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