From Jonathan.Cremer <@t> med.kuleuven.be Tue Apr 1 04:33:21 2014 From: Jonathan.Cremer <@t> med.kuleuven.be (Jonathan Cremer) Date: Tue Apr 1 04:33:33 2014 Subject: [Histonet] Cell block preparation (from cultured cells) Message-ID: Hi everyone, Someone in the lab wishes to look at cultured cells in paraffin embedded cell blocks. These cells grow mostly in non-adherent clumps or cell bodies, which need to be preserved. Sections will be stained by IHC. I've been looking into the matter, and it seems there is not really a single straightforward method for this (as is always the case in histoland, apparently). I've been thinking to try fixing the cells in either 10% alcoholic formalin for 12-24 hours, or zinc-formalin. Then embed in agarose, fix some more with buffered formaldehyde and process. - What are your thoughts on the fixative of choice? - Should I process slowly, manually; or is a 'standard' ON processor protocol adequate (I don't know the exact timings, but I think it's a twelve hour protocol on a caroussel type processor). - Would Histogel have any added benefit over agarose? Thank you, Jonathan --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven From roxannes40 <@t> gmail.com Tue Apr 1 09:20:46 2014 From: roxannes40 <@t> gmail.com (Roxanne) Date: Tue Apr 1 09:20:54 2014 Subject: [Histonet] Renal Lab Volume Message-ID: <8CB69FFF-133B-427A-A39A-BE864DFE179F@gmail.com> Good morning everyone- Quick question, for those that do renal biopsies how many cases would you expect a team of 4 1/2 techs plus a lab aide to be able to handle in a given day? I mean the entire shabang! Light microscopy, frozens, IMF, EM. While I am at it, what about the same for muscle and nerve cases- different groups of people but same question. Thanks in advance Roxanne Sent from my iPhone From rsrichmond <@t> gmail.com Tue Apr 1 12:04:40 2014 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Tue Apr 1 12:04:46 2014 Subject: [Histonet] Re: Bone Marrow smears sent for Giemsa staining Message-ID: Sara Stevens HTL (ASCP) at ACL Laboratories (where?) asks: >>Has anyone experienced poor staining of bone marrow smears that were sent from a doctor's office to a central laboratory? We have some cases of poor staining that we are thinking may be from fume contamination from the fixative bottle. Has anyone experienced anything of this nature?<< A common problem with submitted marrow specimens is that they've been collected in heparin. The oncologist will claim that this technique doesn't damage the specimen, but it does, and there's no work-around for it. Bob Richmond Samurai Pathologist Maryville TN From Pat.Patterson <@t> propath.com Tue Apr 1 13:09:14 2014 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Tue Apr 1 13:09:18 2014 Subject: [Histonet] IHC Supervisor Nights Dallas Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D167DE20C@Mail.propathlab.com> SUPERVISOR, IMMUNOHISTOCHEMISTRY ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an experienced Immunohistochemistry Supervisor to oversee the operations of our night shift. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, In Situ Hybridization procedures, antibody titer preparation, workflow management, equipment maintenance, QC/QA recording, employee assignments. Our desired candidate needs to have strong management abilities; organizational and training skills; a minimum of 5 years of experience in histology, 3 years of IHC experience and with at least three years of supervisory experience; as well as excellent written and oral communication skills. In addition, experience with Microsoft Office and database management is required. We also require (ASCP) HT or HTL certification, IHC certification is encouraged. Minimum of Associates degree required. The hours for the position are 7:00 p.m. to 3:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology practice. Competitive salary with a sign-on bonus and relocation assistance offered. Our benefits include vacation, group medical/dental, and disability insurance, as well as a matched 401(k) plan and more. Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE M/F/Disabled/Veteran Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility@propath.com. Pat Patterson, HTL(ASCP) Manager, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From nicole <@t> dlcjax.com Tue Apr 1 13:58:53 2014 From: nicole <@t> dlcjax.com (nicole@dlcjax.com) Date: Tue Apr 1 13:59:00 2014 Subject: [Histonet] Mercedes Medical Message-ID: <1769836215.110797.1396378733306.JavaMail.mail@webmail06> To those of you who are looking to lower your cost, receive excellent customer service, and enjoy a sweet treat, call my dear friend Chad at Mercedes Medical and let him tell you all about their wonderful deals. Let him send you a product catalog on Mohs products, Clinical products, and Derm products. Their website is super user friendly and contains lots of picture and details about their products. Tell Chad I said Hi!! Mercdedes Medical 18003312716 ext 209 From randycarp5 <@t> gmail.com Tue Apr 1 14:10:47 2014 From: randycarp5 <@t> gmail.com (Randall Carpenter) Date: Tue Apr 1 14:10:50 2014 Subject: [Histonet] Software for old shut/mark printer In-Reply-To: References: Message-ID: Dear Histonet, I was wondering if anyone has software for an older shur/mark cassette printer. I was told that this printer is the second printer they made. The software would be on floppy disks. The machine seems to be in working order, so it would be shame to toss it. Thanks. Randy Carpenter Twin Cities Histology From tony.henwood <@t> health.nsw.gov.au Tue Apr 1 20:07:48 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue Apr 1 20:08:10 2014 Subject: [Histonet] Mercedes Medical In-Reply-To: <1769836215.110797.1396378733306.JavaMail.mail@webmail06> References: <1769836215.110797.1396378733306.JavaMail.mail@webmail06> Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00BC50F@xmdb04.nch.kids> No Adds on Histonet!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nicole@dlcjax.com Sent: Wednesday, 2 April 2014 5:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mercedes Medical To those of you who are looking to lower your cost, receive excellent customer service, and enjoy a sweet treat, call my dear friend Chad at Mercedes Medical and let him tell you all about their wonderful deals. Let him send you a product catalog on Mohs products, Clinical products, and Derm products. Their website is super user friendly and contains lots of picture and details about their products. Tell Chad I said Hi!! Mercdedes Medical 18003312716 ext 209 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From idimitro <@t> mun.ca Wed Apr 2 07:24:49 2014 From: idimitro <@t> mun.ca (idimitro@mun.ca) Date: Wed Apr 2 07:24:56 2014 Subject: [Histonet] suppliers of reusable steel knives Message-ID: <14C3108E8B98EF43B3EDAE5833670034014B444FDE@exchange.med.mun.ca> Hello, I am looking for suppliers of reusable stainless steel knives, the big ones, not the disposable kind. If anyone is still using them and has information where I can buy them and sharpen the knives, it would be of great help for us. Thanks, Iliana Dimitrova, RT, B.Tech., M.Sc. Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 From PAMarcum <@t> uams.edu Wed Apr 2 07:53:10 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Apr 2 07:53:20 2014 Subject: [Histonet] RE: suppliers of reusable steel knives In-Reply-To: <14C3108E8B98EF43B3EDAE5833670034014B444FDE@exchange.med.mun.ca> References: <14C3108E8B98EF43B3EDAE5833670034014B444FDE@exchange.med.mun.ca> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32010DFB6609@Mail2Node2.ad.uams.edu> Try Delaware Diamond Knives or Dorner (Think). There are several if you Google just Histology Steel Knives. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of idimitro@mun.ca Sent: Wednesday, April 02, 2014 7:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] suppliers of reusable steel knives Hello, I am looking for suppliers of reusable stainless steel knives, the big ones, not the disposable kind. If anyone is still using them and has information where I can buy them and sharpen the knives, it would be of great help for us. Thanks, Iliana Dimitrova, RT, B.Tech., M.Sc. Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From lpwenk <@t> sbcglobal.net Wed Apr 2 07:56:09 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Apr 2 07:56:13 2014 Subject: [Histonet] suppliers of reusable steel knives In-Reply-To: <14C3108E8B98EF43B3EDAE5833670034014B444FDE@exchange.med.mun.ca> References: <14C3108E8B98EF43B3EDAE5833670034014B444FDE@exchange.med.mun.ca> Message-ID: We always bought ours from Dorn and Hart, out of Chicago, Illinois. http://www.dornandhart.com/ Peggy Wenk, HTL(ASCP)SLS -----Original Message----- From: idimitro@mun.ca Sent: Wednesday, April 02, 2014 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] suppliers of reusable steel knives Hello, I am looking for suppliers of reusable stainless steel knives, the big ones, not the disposable kind. If anyone is still using them and has information where I can buy them and sharpen the knives, it would be of great help for us. Thanks, Iliana Dimitrova, RT, B.Tech., M.Sc. Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbrya <@t> lexclin.com Wed Apr 2 16:01:15 2014 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Wed Apr 2 16:01:21 2014 Subject: [Histonet] PA hours Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF4C035F5A36@EXCHANGESB> What hours are the PAs are grossing at your locations? The way our specimens arrive into the laboratory, we have someone from 12:30 -9:00 PM. Are any of your PAs working second or third shift hours? Thanks for any input. Regards, Carol NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From BZIMMERM <@t> gru.edu Wed Apr 2 15:51:48 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Wed Apr 2 16:06:24 2014 Subject: [Histonet] HISTOPALOOZA Georgia Society for Histotechnology April 25 - April 27 Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8453F9@EX-MLB-03.ad.georgiahealth.edu> It's been awhile since I've been on the histonet, but I wanted to give you an update about the Histopalooza. We have sold out our block of rooms, but the GSH has acquired a few adjoining cottages at Southern Pine. Please contact 1-800-CALLAWAY and give the symposium code GSH/HISTOPALOOZA! Each one bedroom is the symposium rate of $135. Please don't procrastinate any longer! We look forward to networking, education, and fun. It's like a family reunion. Here's a quote from an unknown author, "Families are like fudge-mostly sweet with a few nuts." The Cary Grant quote, "Insanity runs in my family, it practically gallops." I can think of many family reunion jokes. Look me up at Callaway and I'll share. All I know is my mother is the cruise director of the guilt trip. Glad Mom doesn't read the histonet. hehe Hope to see all of you at the end of the month. Billie Zimmerman GSH secretary From lpwenk <@t> sbcglobal.net Wed Apr 2 17:46:48 2014 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Apr 2 17:46:53 2014 Subject: [Histonet] HISTOPALOOZA Georgia Society for Histotechnology April 25 - April 27 In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF8453F9@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF8453F9@EX-MLB-03.ad.georgiahealth.edu> Message-ID: One of my favorite Cary Grant odd ball comedies - Arsenic and Old Lace. He's the only person I know of who can pull a triple look. If you have never seen this comedy - rent it or down load it or whatever. But be glad Raymond Massey isn't your brother (looking like Boris Karloff), and Peter Lorre isn't his doctor. And your Uncle Teddy doesn't think he's Teddy Roosevelt, trumpeting CHARGE up an stairs (San Juan Hill) and burying victims of yellow fever in the basement, but who were actually lonely old men killed by the 2 old aunts with arsenic laced elderberry wine as part of their charity. It is a hoot! Hope your state meeting family reunion is just as crazy! And I'm a little tea pot! Peggy Wenk, HTL(ASCP)SLS -----Original Message----- From: Zimmerman, Billie Sent: Wednesday, April 02, 2014 4:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HISTOPALOOZA Georgia Society for Histotechnology April 25 - April 27 It's been awhile since I've been on the histonet, but I wanted to give you an update about the Histopalooza. We have sold out our block of rooms, but the GSH has acquired a few adjoining cottages at Southern Pine. Please contact 1-800-CALLAWAY and give the symposium code GSH/HISTOPALOOZA! Each one bedroom is the symposium rate of $135. Please don't procrastinate any longer! We look forward to networking, education, and fun. It's like a family reunion. Here's a quote from an unknown author, "Families are like fudge-mostly sweet with a few nuts." The Cary Grant quote, "Insanity runs in my family, it practically gallops." I can think of many family reunion jokes. Look me up at Callaway and I'll share. All I know is my mother is the cruise director of the guilt trip. Glad Mom doesn't read the histonet. hehe Hope to see all of you at the end of the month. Billie Zimmerman GSH secretary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histopatty <@t> aol.com Thu Apr 3 07:42:51 2014 From: histopatty <@t> aol.com (histopatty@aol.com) Date: Thu Apr 3 07:43:01 2014 Subject: [Histonet] Fwd: [4] Message-ID: <8D11D515C5BC9C8-1B74-1F438@webmail-vm031.sysops.aol.com> Hey! http://labidiomasaiac.usb.ve/-hit.of.sales?quhuniq=7590994&ivyvyzj=922180 From BergerR1 <@t> email.chop.edu Thu Apr 3 09:30:40 2014 From: BergerR1 <@t> email.chop.edu (Berger, Rebecca) Date: Thu Apr 3 09:30:46 2014 Subject: [Histonet] Slide Warmer Message-ID: <5A590EB108038B4FA01F8CDB39C6541B013279A5@EXCMBXPW7.chop.edu> Can anyone recommend a good slide warmer? The more slides it can hold, the better. Thanks, Becky From gtharp <@t> pcasoutheast.com Thu Apr 3 09:59:26 2014 From: gtharp <@t> pcasoutheast.com (Gloria Tharp) Date: Thu Apr 3 09:59:33 2014 Subject: [Histonet] Validation of cryostat Message-ID: <02a201cf4f4d$4ecf2c70$ec6d8550$@com> Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. Gloria Tharp, BA, HTL(ASCP) PCA Southeast Laboratory Director of Operations gtharp@pcasoutheast.com 931-490-1005 931-619-5149 cell From LMurphy2 <@t> aultman.com Thu Apr 3 10:26:17 2014 From: LMurphy2 <@t> aultman.com (Leann M. Murphy) Date: Thu Apr 3 10:26:29 2014 Subject: [Histonet] (no subject) Message-ID: How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? LeAnn Murphy Aultman Hospital Canton, Ohio From joelleweaver <@t> hotmail.com Thu Apr 3 11:03:00 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Apr 3 11:03:05 2014 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: What I did recently for two new processors, conventional type- I did parallel trial slides of multiple tissue types ( same types as patients) for fixation, morphology , processing artifacts for 9 programs. I grossed them in and recorded fixation times, type, thickness, overall dimensions. I ran on the test programs. Then I embedded and sectioned and evaluated the results by microscopic review by techs & then the medical director of the H & E stained sections for each program and tissue type. Looking at any autolysis, nuclear detail, poor dehydration, other processing problems in each set. Then I just made a simple evaluation sheet for any tissue processing related issues, with a number rating/scale for the results. Retained records of the validation runs and the stained sections used for validation. Defined acceptable tissue types and dimensions for the processing programs in the SOP, and then I just created back up/recovery procedure and reprocessing procedure and ran through those for comparison. When completed, I just compiled into validation summary report. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: LMurphy2@aultman.com > To: Histonet@lists.utsouthwestern.edu > Date: Thu, 3 Apr 2014 15:26:17 +0000 > CC: > Subject: [Histonet] (no subject) > > How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? > > > > LeAnn Murphy > > Aultman Hospital > > Canton, Ohio > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vlmckaughan <@t> ccmh.org Thu Apr 3 11:28:42 2014 From: vlmckaughan <@t> ccmh.org (Vicki McKaughan) Date: Thu Apr 3 11:28:48 2014 Subject: [Histonet] proceesing of GI Bxs Message-ID: Could someone please send me your processing schedule for GI bxs. We are using a Sakura VIP5 and are having problems with over drying and occasionally with infiltration of paraffin. Thanks From arguellod <@t> ihn.org Thu Apr 3 11:33:06 2014 From: arguellod <@t> ihn.org (Arguello, Daniel) Date: Thu Apr 3 11:33:21 2014 Subject: [Histonet] RE: proceesing of GI Bxs In-Reply-To: References: Message-ID: <46FD76128C5055478050297AEAC97E17343CB73A@EXCHANGE-1.corporate.lan> I'd appreciate a copy of that processing schedule for GI bxs as well. Thank you in advance Sincerely,? Daniel Arg?ello, BS, CT (ASCP)CM Anatomic Pathology Services Coordinator Inspira Medical Center Woodbury 509 North Broad Street Woodbury, NJ 08096 856-853-2030 Ext 2808 856-853-2183 Fax arguellod@ihn.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vicki McKaughan Sent: Thursday, April 03, 2014 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] proceesing of GI Bxs Could someone please send me your processing schedule for GI bxs. We are using a Sakura VIP5 and are having problems with over drying and occasionally with infiltration of paraffin. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Thu Apr 3 12:42:14 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Apr 3 12:42:19 2014 Subject: [Histonet] RE: PA hours In-Reply-To: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> Message-ID: We are a small lab, avg. 200/blocks/day. Our PA comes in at 0800, cleans up the gross room, cryostat, etc and usually starts with the placenta's from the previous night's deliveries. Also, she does all the frozen, and since our OR's start at 0730, we often have frozen by 0800 or soon after. I think it just all depends on the extra duties and when your Ors run. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- Sent: Thursday, April 03, 2014 12:22 PM 1. PA hours (Carol Bryant) Message: 1 Date: Wed, 2 Apr 2014 17:01:15 -0400 From: Carol Bryant Subject: [Histonet] PA hours What hours are the PAs are grossing at your locations? The way our specimens arrive into the laboratory, we have someone from 12:30 -9:00 PM. Are any of your PAs working second or third shift hours? Thanks for any input. Regards, Carol ********************************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From cmiller <@t> physlab.com Thu Apr 3 12:47:47 2014 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Apr 3 12:47:54 2014 Subject: [Histonet] Sausage blocks and tissue arrays Message-ID: What is everyone doing for IHC control tissue? My path that made my sausage blocks is no longer here and reality has kicked in! I need to find a suitable replacement. Is anyone making their own control block or "Sausage" without using the microarray punch and paraffin block? Thanks, Cheri Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From karen <@t> gateslinger.com Thu Apr 3 12:48:06 2014 From: karen <@t> gateslinger.com (Karen Lahti) Date: Thu Apr 3 12:48:12 2014 Subject: [Histonet] RE: proceesing of GI Bxs In-Reply-To: <46FD76128C5055478050297AEAC97E17343CB73A@EXCHANGE-1.corporate.lan> References: <46FD76128C5055478050297AEAC97E17343CB73A@EXCHANGE-1.corporate.lan> Message-ID: We have a medium GI lab using the old, old VIP's. We have been very successful with two processing schedules, long and short. We found that we needed to add a bit of heat to the last xylene so when the tissue hit the first paraffin, they would start to infiltrate. The cold cassettes solidified the paraffin and the time spent was melting the paraffin not infiltrating the biopsy. For the longer run, we add 10%NBF and a bit longer in each solution. Short: 1. 70% reagent alcohol - 15 min, 40C, P/V 2. 85% reagent alcohol - 10 min, 40C, P/V 3. 85% reagent alcohol - 15 min, 40C, P/V 4. 100% reagent alcohol - 10 min, 40C, P/V 5. 100% reagent alcohol - 10 min, 40C, P/V 6. 100% reagent alcohol - 10 min, 40C, P/V 7. Xylene - 20 min, 40C, P/V 8. Xylene - 20 min, 50C, P/V 9. Paraffin - 10 min, 58C, P/V 10. Paraffin - 30 min, 58C, P/V 11. Paraffin - 45 min, 58C, P/V Karen Lahti, HT (ASCP), QIHC Arizona Digestive Health 602-687-7468 On Apr 3, 2014, at 9:33 AM, "Arguello, Daniel" wrote: > I'd appreciate a copy of that processing schedule for GI bxs as well. > Thank you in advance > > Sincerely, > > Daniel Arg?ello, BS, CT (ASCP)CM > Anatomic Pathology Services Coordinator > Inspira Medical Center Woodbury > 509 North Broad Street > Woodbury, NJ 08096 > 856-853-2030 Ext 2808 > 856-853-2183 Fax > arguellod@ihn.org > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vicki McKaughan > Sent: Thursday, April 03, 2014 12:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] proceesing of GI Bxs > > Could someone please send me your processing schedule for GI bxs. We are using a Sakura VIP5 and are having problems with over drying and occasionally with infiltration of paraffin. Thanks _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Thu Apr 3 13:00:21 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Apr 3 13:00:25 2014 Subject: [Histonet] RE: Instrument Verification In-Reply-To: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> Message-ID: I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 6. Validation of cryostat (Gloria Tharp) Message: 6 Date: Thu, 3 Apr 2014 09:59:26 -0500 From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. Gloria Tharp, BA, HTL(ASCP) ------------------------------ Message: 7 Date: Thu, 3 Apr 2014 15:26:17 +0000 From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? LeAnn Murphy Aultman Hospital Canton, Ohio ************************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From doug.porter <@t> caplab.org Thu Apr 3 13:06:41 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Thu Apr 3 13:05:28 2014 Subject: [Histonet] RE: PA hours In-Reply-To: References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> Message-ID: <001801cf4f67$7802f800$6808e800$@caplab.org> PA's will work when the specimens are in the lab. As previously mentioned, if it's a hospital only lab, then a conventional day shift would work. After all, what surgeon wants to work past 4p? LOL!! However, if the lab services a hospital, surgery centers, doctor offices, etc., the PA or PA's will work when those specimens are available which usually means during the day, in the late afternoon and early evening. Mine is the later example. The specimens don't start arriving until 130p and keep coming until 630p. I have couriers dropping off specimens throughout the afternoon. I do supervisory work, coordinate the IT infrastructure, submit all of our primary malignant cases to our State Cancer Registry and in my spare time gross all of the specimens. I work from Noon to 830p or later. So, Carol...it all depends on the workload and when the work is received. Hope this helps!! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, April 03, 2014 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: PA hours We are a small lab, avg. 200/blocks/day. Our PA comes in at 0800, cleans up the gross room, cryostat, etc and usually starts with the placenta's from the previous night's deliveries. Also, she does all the frozen, and since our OR's start at 0730, we often have frozen by 0800 or soon after. I think it just all depends on the extra duties and when your Ors run. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- Sent: Thursday, April 03, 2014 12:22 PM 1. PA hours (Carol Bryant) Message: 1 Date: Wed, 2 Apr 2014 17:01:15 -0400 From: Carol Bryant Subject: [Histonet] PA hours What hours are the PAs are grossing at your locations? The way our specimens arrive into the laboratory, we have someone from 12:30 -9:00 PM. Are any of your PAs working second or third shift hours? Thanks for any input. Regards, Carol ********************************** ---------------------------------------------------------------------------- ----- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3722/7293 - Release Date: 04/03/14 From robinsoc <@t> mercyhealth.com Thu Apr 3 14:03:24 2014 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Apr 3 14:03:32 2014 Subject: [Histonet] RE: Instrument Verification In-Reply-To: References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> Message-ID: <4EE642D353925D4D96CB95E12427DBAE5C90FE@NODCMSTMBX06.no.trinity-health.org> I agree with you in that CAP is just looking for things to change and doesn't seem to be considering the change and decrease in staffing seen in clinical settings. Cryostat validation? Really....cut a slide after you have cleaned and pm'd the thing and go on. Good grief...I don't need any more paper and documentation on routine processes. As for tissue processors, I have 20 year old VIP's that have been running and producing specimens acceptably. I did validate them prior to being put in use but we didn't document like we do now. And I don't see the need to do it at this stage of use. We did do a very extensive validation on the Peloris we put into use last year and will going forward on new equipment. To me the daily QC of stain should provide our 'validation' of the process and include the processor. I am interested in others thoughts as well. Thanks for allowing me to rant. Cindi Robinson, HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Drive Dakota Dunes SD 57049 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, April 03, 2014 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 6. Validation of cryostat (Gloria Tharp) Message: 6 Date: Thu, 3 Apr 2014 09:59:26 -0500 From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. Gloria Tharp, BA, HTL(ASCP) ------------------------------ Message: 7 Date: Thu, 3 Apr 2014 15:26:17 +0000 From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? LeAnn Murphy Aultman Hospital Canton, Ohio ************************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Thu Apr 3 14:22:13 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Apr 3 14:22:21 2014 Subject: [Histonet] RE: Instrument Verification In-Reply-To: <4EE642D353925D4D96CB95E12427DBAE5C90FE@NODCMSTMBX06.no.trinity-health.org> References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> <4EE642D353925D4D96CB95E12427DBAE5C90FE@NODCMSTMBX06.no.trinity-health.org> Message-ID: I'm with you. There really appears to be no value to this particular requirement. I would only be concerned with it if I had just purchased it, or moved it into our lab from another location. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, April 03, 2014 3:03 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I agree with you in that CAP is just looking for things to change and doesn't seem to be considering the change and decrease in staffing seen in clinical settings. Cryostat validation? Really....cut a slide after you have cleaned and pm'd the thing and go on. Good grief...I don't need any more paper and documentation on routine processes. As for tissue processors, I have 20 year old VIP's that have been running and producing specimens acceptably. I did validate them prior to being put in use but we didn't document like we do now. And I don't see the need to do it at this stage of use. We did do a very extensive validation on the Peloris we put into use last year and will going forward on new equipment. To me the daily QC of stain should provide our 'validation' of the process and include the processor. I am interested in others thoughts as well. Thanks for allowing me to rant. Cindi Robinson, HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Drive Dakota Dunes SD 57049 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, April 03, 2014 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 6. Validation of cryostat (Gloria Tharp) Message: 6 Date: Thu, 3 Apr 2014 09:59:26 -0500 From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. Gloria Tharp, BA, HTL(ASCP) ------------------------------ Message: 7 Date: Thu, 3 Apr 2014 15:26:17 +0000 From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? LeAnn Murphy Aultman Hospital Canton, Ohio ************************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Apr 3 15:16:58 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 3 15:17:08 2014 Subject: [Histonet] RE: Instrument Verification In-Reply-To: References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> <4EE642D353925D4D96CB95E12427DBAE5C90FE@NODCMSTMBX06.no.trinity-health.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E057D4@SBS2K8.premierlab.local> Hello All Coming from a GLP environment this type of equipment validation is standard in our setting. This is just my opinion but I think the CAP checklist is moving towards the type of equipment documentation that is already required in a GxP or ISO environment. I always thought that instrument qualification (IQ) - operational qualification (OQ) and process qualification (PQ) or simply stated IQ/OQ/PQ were used only in GxP settings but you now see some of the larger clinical labs running these types of validations on their equipment and processes. To me it does make sense that some type of equipment validation should be required whether it is a two page document on the microtomes, waterbaths, etc. or complete IQ/OQ/PQ's on major pieces of equipment such as tissue processors, immunostainers and IHC retrieval units. I believe that all of these are important processes that should be completed in histology laboratories today. We are a GLP compliant lab and every single piece of equipment is calibrated and validated as designated in our Master Validation Plan. IHC stainers and retrieval units should be validated, even our refrigerators and freezers are calibrated and validated. Our pipettors are calibrated quarterly, and any piece of equipment that generates a weight or temperature is calibrated yearly. For example if you do not validate your IHC retrieval units how can you really tell if they reach the temperature that they are programmed to reach, does the temperature stay consistent through the retrieval process, did it retrieve for the time programmed? The only way to determine this is to perform a validation. How do you troubleshoot problems if you do not know if your instruments are performing to their specification without testing those specifications - that's what equipment validation is and that's why in my opinion its important. Histology laboratories are now responsible for running IHC that directly effects a patients treatment - meaning the numerous therapeutic and prognostic markers we routinely run now. Validation is an important process especially if you are using image analysis for these markers. I hate to say it but we better get used to it, because this is not going away. And now the shameless plug - I will be giving a 90 minute lecture at the Florida State Meeting https://classic.regonline.com/custImages/240000/241449/FSH2014OnlineProgram.pdf on this exact topic, so if you want to learn how to create a Master Validation Plan and learn how to perform a basic validation or a more detailed IQ/OQ and PQ and to what extent you need to validate a particular piece of equipment - sign up for the meeting plus there are lots of other great topics being presented too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, April 03, 2014 1:22 PM To: Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I'm with you. There really appears to be no value to this particular requirement. I would only be concerned with it if I had just purchased it, or moved it into our lab from another location. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, April 03, 2014 3:03 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I agree with you in that CAP is just looking for things to change and doesn't seem to be considering the change and decrease in staffing seen in clinical settings. Cryostat validation? Really....cut a slide after you have cleaned and pm'd the thing and go on. Good grief...I don't need any more paper and documentation on routine processes. As for tissue processors, I have 20 year old VIP's that have been running and producing specimens acceptably. I did validate them prior to being put in use but we didn't document like we do now. And I don't see the need to do it at this stage of use. We did do a very extensive validation on the Peloris we put into use last year and will going forward on new equipment. To me the daily QC of stain should provide our 'validation' of the process and include the processor. I am interested in others thoughts as well. Thanks for allowing me to rant. Cindi Robinson, HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Drive Dakota Dunes SD 57049 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, April 03, 2014 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 6. Validation of cryostat (Gloria Tharp) Message: 6 Date: Thu, 3 Apr 2014 09:59:26 -0500 From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. Gloria Tharp, BA, HTL(ASCP) ------------------------------ Message: 7 Date: Thu, 3 Apr 2014 15:26:17 +0000 From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? LeAnn Murphy Aultman Hospital Canton, Ohio ************************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Apr 3 18:51:54 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Apr 3 18:51:59 2014 Subject: [Histonet] RE: Instrument Verification In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E057D4@SBS2K8.premierlab.local> References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local>, , <4EE642D353925D4D96CB95E12427DBAE5C90FE@NODCMSTMBX06.no.trinity-health.org>, , <14E2C6176416974295479C64A11CB9AE019C79E057D4@SBS2K8.premierlab.local> Message-ID: I do understand and sympathize with the situation in many clinical labs with staff , sometimes barely enough to do the work and it is challenging to keep up with expanding documentation also. I would like to meet the GLP, but do struggle to be as extensive in my documentation. I do try to get as close to the ISO standards as possible, just to cover myself. I agree with Elizabeth's post that this seems to be the direction CAP has been heading over the years. I think that if you get new instruments, methodology, technology they will certainly want to see the more robust documentation. For example ( see the current CAP today on IHC validation), this will surely be the "guideline" of tomorrow.... But for those older, in long use instruments and technology, my opinion is that if you have documentation in line with what the checklist stipulated when it went into use, and also all PM, maintenance, and QC- and have documented any corrective actions, this will probably "fly" for now? What does everyone else think? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: liz@premierlab.com > To: mward@wakehealth.edu; robinsoc@mercyhealth.com; tbraud@holyredeemer.com; histonet@lists.utsouthwestern.edu > Date: Thu, 3 Apr 2014 14:16:58 -0600 > CC: > Subject: [Histonet] RE: Instrument Verification > > Hello All > > Coming from a GLP environment this type of equipment validation is standard in our setting. This is just my opinion but I think the CAP checklist is moving towards the type of equipment documentation that is already required in a GxP or ISO environment. I always thought that instrument qualification (IQ) - operational qualification (OQ) and process qualification (PQ) or simply stated IQ/OQ/PQ were used only in GxP settings but you now see some of the larger clinical labs running these types of validations on their equipment and processes. To me it does make sense that some type of equipment validation should be required whether it is a two page document on the microtomes, waterbaths, etc. or complete IQ/OQ/PQ's on major pieces of equipment such as tissue processors, immunostainers and IHC retrieval units. I believe that all of these are important processes that should be completed in histology laboratories today. We are a GLP compliant lab and every single piece of equipment is calibrated and validated as designated in our Master Validation Plan. IHC stainers and retrieval units should be validated, even our refrigerators and freezers are calibrated and validated. Our pipettors are calibrated quarterly, and any piece of equipment that generates a weight or temperature is calibrated yearly. > > For example if you do not validate your IHC retrieval units how can you really tell if they reach the temperature that they are programmed to reach, does the temperature stay consistent through the retrieval process, did it retrieve for the time programmed? The only way to determine this is to perform a validation. How do you troubleshoot problems if you do not know if your instruments are performing to their specification without testing those specifications - that's what equipment validation is and that's why in my opinion its important. > > Histology laboratories are now responsible for running IHC that directly effects a patients treatment - meaning the numerous therapeutic and prognostic markers we routinely run now. Validation is an important process especially if you are using image analysis for these markers. I hate to say it but we better get used to it, because this is not going away. > > And now the shameless plug - I will be giving a 90 minute lecture at the Florida State Meeting https://classic.regonline.com/custImages/240000/241449/FSH2014OnlineProgram.pdf on this exact topic, so if you want to learn how to create a Master Validation Plan and learn how to perform a basic validation or a more detailed IQ/OQ and PQ and to what extent you need to validate a particular piece of equipment - sign up for the meeting plus there are lots of other great topics being presented too. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology > Sent: Thursday, April 03, 2014 1:22 PM > To: Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Instrument Verification > > I'm with you. There really appears to be no value to this particular requirement. I would only be concerned with it if I had just purchased it, or moved it into our lab from another location. > > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson > Sent: Thursday, April 03, 2014 3:03 PM > To: Terri Braud; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Instrument Verification > > I agree with you in that CAP is just looking for things to change and doesn't seem to be considering the change and decrease in staffing seen in clinical settings. Cryostat validation? Really....cut a slide after you have cleaned and pm'd the thing and go on. Good grief...I don't need any more paper and documentation on routine processes. As for tissue processors, I have 20 year old VIP's that have been running and producing specimens acceptably. I did validate them prior to being put in use but we didn't document like we do now. And I don't see the need to do it at this stage of use. We did do a very extensive validation on the Peloris we put into use last year and will going forward on new equipment. To me the daily QC of stain should provide our 'validation' of the process and include the processor. I am interested in others thoughts as well. > > Thanks for allowing me to rant. > > Cindi Robinson, HT(ASCP) > Mercy Medical Center-Sioux City > Dunes Medical Laboratories > 350 W Anchor Drive > Dakota Dunes SD 57049 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud > Sent: Thursday, April 03, 2014 1:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Instrument Verification > > I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > 6. Validation of cryostat (Gloria Tharp) > > Message: 6 > Date: Thu, 3 Apr 2014 09:59:26 -0500 > From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. > Gloria Tharp, BA, HTL(ASCP) > ------------------------------ > > Message: 7 > Date: Thu, 3 Apr 2014 15:26:17 +0000 > From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? > LeAnn Murphy > Aultman Hospital > Canton, Ohio > ************************ > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From millers <@t> emhs.org Fri Apr 4 05:08:54 2014 From: millers <@t> emhs.org (Miller, Suzie) Date: Fri Apr 4 05:09:04 2014 Subject: [Histonet] RE: Instrument Verification In-Reply-To: References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local>, , <4EE642D353925D4D96CB95E12427DBAE5C90FE@NODCMSTMBX06.no.trinity-health.org>, , <14E2C6176416974295479C64A11CB9AE019C79E057D4@SBS2K8.premierlab.local> Message-ID: I must say, the first time I read ANP.23045 I had the same reaction you all did. After reading it over a couple of times, we started looking at it differently. The question is not about validation--- it is about verifying that the equipment will function and perform as intended. It doesn't have anything to do with protocols and procedures for staining, cutting, processing, etc. If you look at any service report for service performed on your instrument, it is likely that the service engineer has some sort of notation on the report that states the unit was run and monitored for performance and meets all specs. To comply with the CAP question we merely wrote a procedure for Instrument-Equipment Performance Verification stating that 1)The verification of instrument/equipment function will be performed by a qualified service engineer upon installation, after scheduled preventative maintenance, major instrument repairs, or relocation. 2) Use of the instrument/equipment will resume after the verification of its performance has been successfully completed.3) The service engineer will provide the Histology Laboratory with a report indicating that performance function was tested and satisfactory. Of course, we also have all service reports (which include the notation that the function checks were performed and acceptable) available for each instrument. Suzie Miller, MLT ASCP Senior Histotech Mercy Health System of Maine Laboratory -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Thursday, April 03, 2014 7:52 PM To: Elizabeth Chlipala; Martha Ward-Pathology; Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Instrument Verification I do understand and sympathize with the situation in many clinical labs with staff , sometimes barely enough to do the work and it is challenging to keep up with expanding documentation also. I would like to meet the GLP, but do struggle to be as extensive in my documentation. I do try to get as close to the ISO standards as possible, just to cover myself. I agree with Elizabeth's post that this seems to be the direction CAP has been heading over the years. I think that if you get new instruments, methodology, technology they will certainly want to see the more robust documentation. For example ( see the current CAP today on IHC validation), this will surely be the "guideline" of tomorrow.... But for those older, in long use instruments and technology, my opinion is that if you have documentation in line with what the checklist stipulated when it went into use, and also all PM, maintenance, and QC- and have documented any corrective actions, this will probably "fly" for now? What does everyone else think? Joelle Weaver MAOM, HTL (ASCP) QIHC > From: liz@premierlab.com > To: mward@wakehealth.edu; robinsoc@mercyhealth.com; > tbraud@holyredeemer.com; histonet@lists.utsouthwestern.edu > Date: Thu, 3 Apr 2014 14:16:58 -0600 > CC: > Subject: [Histonet] RE: Instrument Verification > > Hello All > > Coming from a GLP environment this type of equipment validation is standard in our setting. This is just my opinion but I think the CAP checklist is moving towards the type of equipment documentation that is already required in a GxP or ISO environment. I always thought that instrument qualification (IQ) - operational qualification (OQ) and process qualification (PQ) or simply stated IQ/OQ/PQ were used only in GxP settings but you now see some of the larger clinical labs running these types of validations on their equipment and processes. To me it does make sense that some type of equipment validation should be required whether it is a two page document on the microtomes, waterbaths, etc. or complete IQ/OQ/PQ's on major pieces of equipment such as tissue processors, immunostainers and IHC retrieval units. I believe that all of these are important processes that should be completed in histology laboratories today. We are a GLP compliant lab and every single piece of equipment is calibrated and validated as designated in our Master Validation Plan. IHC stainers and retrieval units should be validated, even our refrigerators and freezers are calibrated and validated. Our pipettors are calibrated quarterly, and any piece of equipment that generates a weight or temperature is calibrated yearly. > > For example if you do not validate your IHC retrieval units how can you really tell if they reach the temperature that they are programmed to reach, does the temperature stay consistent through the retrieval process, did it retrieve for the time programmed? The only way to determine this is to perform a validation. How do you troubleshoot problems if you do not know if your instruments are performing to their specification without testing those specifications - that's what equipment validation is and that's why in my opinion its important. > > Histology laboratories are now responsible for running IHC that directly effects a patients treatment - meaning the numerous therapeutic and prognostic markers we routinely run now. Validation is an important process especially if you are using image analysis for these markers. I hate to say it but we better get used to it, because this is not going away. > > And now the shameless plug - I will be giving a 90 minute lecture at the Florida State Meeting https://classic.regonline.com/custImages/240000/241449/FSH2014OnlineProgram.pdf on this exact topic, so if you want to learn how to create a Master Validation Plan and learn how to perform a basic validation or a more detailed IQ/OQ and PQ and to what extent you need to validate a particular piece of equipment - sign up for the meeting plus there are lots of other great topics being presented too. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > Ward-Pathology > Sent: Thursday, April 03, 2014 1:22 PM > To: Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Instrument Verification > > I'm with you. There really appears to be no value to this particular requirement. I would only be concerned with it if I had just purchased it, or moved it into our lab from another location. > > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ > f 336.716.5890 mward@wakehealth.edu > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Cynthia Robinson > Sent: Thursday, April 03, 2014 3:03 PM > To: Terri Braud; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Instrument Verification > > I agree with you in that CAP is just looking for things to change and doesn't seem to be considering the change and decrease in staffing seen in clinical settings. Cryostat validation? Really....cut a slide after you have cleaned and pm'd the thing and go on. Good grief...I don't need any more paper and documentation on routine processes. As for tissue processors, I have 20 year old VIP's that have been running and producing specimens acceptably. I did validate them prior to being put in use but we didn't document like we do now. And I don't see the need to do it at this stage of use. We did do a very extensive validation on the Peloris we put into use last year and will going forward on new equipment. To me the daily QC of stain should provide our 'validation' of the process and include the processor. I am interested in others thoughts as well. > > Thanks for allowing me to rant. > > Cindi Robinson, HT(ASCP) > Mercy Medical Center-Sioux City > Dunes Medical Laboratories > 350 W Anchor Drive > Dakota Dunes SD 57049 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri > Braud > Sent: Thursday, April 03, 2014 1:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Instrument Verification > > I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > 6. Validation of cryostat (Gloria Tharp) > > Message: 6 > Date: Thu, 3 Apr 2014 09:59:26 -0500 > From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. > Gloria Tharp, BA, HTL(ASCP) > ------------------------------ > > Message: 7 > Date: Thu, 3 Apr 2014 15:26:17 +0000 > From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? > LeAnn Murphy > Aultman Hospital > Canton, Ohio > ************************ > ---------------------------------------------------------------------- > ----------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------------- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. From joelleweaver <@t> hotmail.com Fri Apr 4 06:43:45 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Apr 4 06:43:52 2014 Subject: [Histonet] RE: Instrument Verification In-Reply-To: References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local>, , , , <4EE642D353925D4D96CB95E12427DBAE5C90FE@NODCMSTMBX06.no.trinity-health.org>, , , , <14E2C6176416974295479C64A11CB9AE019C79E057D4@SBS2K8.premierlab.local>, , Message-ID: Pretty much what is in place here for existing/established instruments. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: millers@emhs.org > To: joelleweaver@hotmail.com; liz@premierlab.com; mward@wakehealth.edu; robinsoc@mercyhealth.com; tbraud@holyredeemer.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Instrument Verification > Date: Fri, 4 Apr 2014 10:08:54 +0000 > > I must say, the first time I read ANP.23045 I had the same reaction you all did. After reading it over a couple of times, we started looking at it differently. The question is not about validation--- it is about verifying that the equipment will function and perform as intended. It doesn't have anything to do with protocols and procedures for staining, cutting, processing, etc. If you look at any service report for service performed on your instrument, it is likely that the service engineer has some sort of notation on the report that states the unit was run and monitored for performance and meets all specs. > To comply with the CAP question we merely wrote a procedure for Instrument-Equipment Performance Verification stating that 1)The verification of instrument/equipment function will be performed by a qualified service engineer upon installation, after scheduled preventative maintenance, major instrument repairs, or relocation. 2) Use of the instrument/equipment will resume after the verification of its performance has been successfully completed.3) The service engineer will provide the Histology Laboratory with a report indicating that performance function was tested and satisfactory. > Of course, we also have all service reports (which include the notation that the function checks were performed and acceptable) available for each instrument. > > Suzie Miller, MLT ASCP > Senior Histotech > > Mercy Health System of Maine Laboratory > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver > Sent: Thursday, April 03, 2014 7:52 PM > To: Elizabeth Chlipala; Martha Ward-Pathology; Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Instrument Verification > > I do understand and sympathize with the situation in many clinical labs with staff , sometimes barely enough to do the work and it is challenging to keep up with expanding documentation also. I would like to meet the GLP, but do struggle to be as extensive in my documentation. I do try to get as close to the ISO standards as possible, just to cover myself. I agree with Elizabeth's post that this seems to be the direction CAP has been heading over the years. > I think that if you get new instruments, methodology, technology they will certainly want to see the more robust documentation. For example ( see the current CAP today on IHC validation), this will surely be the "guideline" of tomorrow.... > But for those older, in long use instruments and technology, my opinion is that if you have documentation in line with what the checklist stipulated when it went into use, and also all PM, maintenance, and QC- and have documented any corrective actions, this will probably "fly" for now? What does everyone else think? > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: liz@premierlab.com > > To: mward@wakehealth.edu; robinsoc@mercyhealth.com; > > tbraud@holyredeemer.com; histonet@lists.utsouthwestern.edu > > Date: Thu, 3 Apr 2014 14:16:58 -0600 > > CC: > > Subject: [Histonet] RE: Instrument Verification > > > > Hello All > > > > Coming from a GLP environment this type of equipment validation is standard in our setting. This is just my opinion but I think the CAP checklist is moving towards the type of equipment documentation that is already required in a GxP or ISO environment. I always thought that instrument qualification (IQ) - operational qualification (OQ) and process qualification (PQ) or simply stated IQ/OQ/PQ were used only in GxP settings but you now see some of the larger clinical labs running these types of validations on their equipment and processes. To me it does make sense that some type of equipment validation should be required whether it is a two page document on the microtomes, waterbaths, etc. or complete IQ/OQ/PQ's on major pieces of equipment such as tissue processors, immunostainers and IHC retrieval units. I believe that all of these are important processes that should be completed in histology laboratories today. We are a GLP compliant lab and every single piece of equipment is calibrated and validated as designated in our Master Validation Plan. IHC stainers and retrieval units should be validated, even our refrigerators and freezers are calibrated and validated. Our pipettors are calibrated quarterly, and any piece of equipment that generates a weight or temperature is calibrated yearly. > > > > For example if you do not validate your IHC retrieval units how can you really tell if they reach the temperature that they are programmed to reach, does the temperature stay consistent through the retrieval process, did it retrieve for the time programmed? The only way to determine this is to perform a validation. How do you troubleshoot problems if you do not know if your instruments are performing to their specification without testing those specifications - that's what equipment validation is and that's why in my opinion its important. > > > > Histology laboratories are now responsible for running IHC that directly effects a patients treatment - meaning the numerous therapeutic and prognostic markers we routinely run now. Validation is an important process especially if you are using image analysis for these markers. I hate to say it but we better get used to it, because this is not going away. > > > > And now the shameless plug - I will be giving a 90 minute lecture at the Florida State Meeting https://classic.regonline.com/custImages/240000/241449/FSH2014OnlineProgram.pdf on this exact topic, so if you want to learn how to create a Master Validation Plan and learn how to perform a basic validation or a more detailed IQ/OQ and PQ and to what extent you need to validate a particular piece of equipment - sign up for the meeting plus there are lots of other great topics being presented too. > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > > Box 18592 Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > liz@premierlab.com > > www.premierlab.com > > > > March 10, 2014 is Histotechnology Professionals Day > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > Longmont, CO 80504 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha > > Ward-Pathology > > Sent: Thursday, April 03, 2014 1:22 PM > > To: Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: Instrument Verification > > > > I'm with you. There really appears to be no value to this particular requirement. I would only be concerned with it if I had just purchased it, or moved it into our lab from another location. > > > > > > > > Martha Ward, MT (ASCP) QIHC > > Manager > > > > Molecular Diagnostics Lab > > Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ > > f 336.716.5890 mward@wakehealth.edu > > > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > Cynthia Robinson > > Sent: Thursday, April 03, 2014 3:03 PM > > To: Terri Braud; histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: Instrument Verification > > > > I agree with you in that CAP is just looking for things to change and doesn't seem to be considering the change and decrease in staffing seen in clinical settings. Cryostat validation? Really....cut a slide after you have cleaned and pm'd the thing and go on. Good grief...I don't need any more paper and documentation on routine processes. As for tissue processors, I have 20 year old VIP's that have been running and producing specimens acceptably. I did validate them prior to being put in use but we didn't document like we do now. And I don't see the need to do it at this stage of use. We did do a very extensive validation on the Peloris we put into use last year and will going forward on new equipment. To me the daily QC of stain should provide our 'validation' of the process and include the processor. I am interested in others thoughts as well. > > > > Thanks for allowing me to rant. > > > > Cindi Robinson, HT(ASCP) > > Mercy Medical Center-Sioux City > > Dunes Medical Laboratories > > 350 W Anchor Drive > > Dakota Dunes SD 57049 > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri > > Braud > > Sent: Thursday, April 03, 2014 1:00 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] RE: Instrument Verification > > > > I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? > > > > Terri L. Braud, HT(ASCP) > > Anatomic Pathology Supervisor > > Holy Redeemer Hospital Laboratory > > 1648 Huntingdon Pike > > Meadowbrook, PA 19046 > > Ph: 215-938-3676 > > Fax: 215-938-3874 > > > > 6. Validation of cryostat (Gloria Tharp) > > > > Message: 6 > > Date: Thu, 3 Apr 2014 09:59:26 -0500 > > From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. > > Gloria Tharp, BA, HTL(ASCP) > > ------------------------------ > > > > Message: 7 > > Date: Thu, 3 Apr 2014 15:26:17 +0000 > > From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? > > LeAnn Murphy > > Aultman Hospital > > Canton, Ohio > > ************************ > > ---------------------------------------------------------------------- > > ----------- > > > > > > > > CONFIDENTIALITY NOTICE: > > > > This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. > > Please notify the sender of any error by E-Mail. > > > > Thank you for your cooperation. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------------------------------------------------------------------------------------------- > This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. From tmcampbell <@t> fmh.org Fri Apr 4 06:57:45 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Fri Apr 4 06:57:42 2014 Subject: [Histonet] GI Biopsies Message-ID: <3566D9E34287BE4B95372179009446A01C16B717@EXCHANGE.fmhnt.fmh.org> Does anyone have problems with their GI biopsies kind of shredding when cutting? I microwave process them in the Shurwave microwave processor. I have been doing this for over a year and only the past couple months I have noticed that almost every biopsy I cut has knife lines in it. I have to change my blade constantly. I was told by a TBS technician that if I ever noticed the tissue doing this, it meant that I did not have all the alcohol out of the tissue, but does that sound like it would cause the tissue to shred like that? I don't use my reagents more than 2 times and I have even tried increasing the time and temp a little but no help. I have been doing them for a year and did not have a problem and I haven't changed anything. Any solutions or suggestions??? Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From celebrej <@t> HHSC.CA Fri Apr 4 07:19:11 2014 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Fri Apr 4 07:20:25 2014 Subject: [Histonet] PFK's on muscle biopsies Message-ID: <722701352B76E841BFB9EFAF72553CD27E9E321C@ipexmailm02.hhsc.ca> Happy Friday !! For years now our PFK staining has been inconsistent and I'm hoping someone wouldn't mind sharing their no fail PFK method so I can make my neuropath's happy again.?? Julia Celebre Sr MLT Anatomic Pathology Hamilton Civic Hospitals 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From TJohnson <@t> gnf.org Fri Apr 4 11:04:37 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Fri Apr 4 11:04:48 2014 Subject: [Histonet] anti-FLAG antibody Message-ID: <9F3CFEE76E51B64991C7485270890B404989307B@EX5.lj.gnf.org> Happy Friday! I am looking for a source of anti-FLAG antibody that works in mouse FFPE tissues. Bonus points if it work with Ventana/Roche instrumentation. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From bellafiore.lori <@t> gene.com Fri Apr 4 11:55:34 2014 From: bellafiore.lori <@t> gene.com (Lori Bellafiore) Date: Fri Apr 4 11:56:25 2014 Subject: [Histonet] 45th Annual Illinois Society for Histotechnologists Meeting May 15 & 16 Message-ID: Hello, It is time to register for the 45th Annual ISH SPring Symposium in Bloomington, IL May 15 & 16, 2014. Please see the following website for complete meeting details and to register online. http://www.illinoishistologysociety.org/meetings.html THank you! -- Lori Ann Bellafiore *Pathology Education Specialist* *Genentech* 217.508.9975 From Vickroy.Jim <@t> mhsil.com Fri Apr 4 12:08:30 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Fri Apr 4 12:08:35 2014 Subject: [Histonet] Ventana and CoPath Inface (HL7 Stain Order Interface) Message-ID: We currently use the ABT system of CoPath Plus and have three Ventana Ultra Stainers and two Benchmark Special Stainers. We have been told that the HL7 Stain Order Interface will save us a lot of clerical time. We are trying to contact someone that already has this interface to see if it is worth the money. Please let us know if you have the interface and your experience with it. If possible maybe we could contact you and get some feedback. Jim V James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From delsuec <@t> gmail.com Fri Apr 4 12:39:05 2014 From: delsuec <@t> gmail.com (Deloris Carter) Date: Fri Apr 4 12:39:08 2014 Subject: [Histonet] Biocare intellipath Message-ID: I'm looking for some honest feedback from anyone using the Biocare Intellipath in a clinical setting. Pros, cons, etc. Thanks, Deloris Carter, HT(ASCP) delsuec@gmail.com From litepath2000 <@t> yahoo.com Sat Apr 5 09:52:56 2014 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Sat Apr 5 09:53:08 2014 Subject: [Histonet] New York State Histotechnological Society 2014 Annual Meeting Message-ID: <1396709576.22416.YahooMailNeo@web140302.mail.bf1.yahoo.com> The New York State Histotechnological Society 2014 Annual meeting will be held May 2nd & 3rd at the Inn on the Lake Waterfront and Conference Center Canandaigua, New York. For program, registration form and hotel & travel, please visit our annual meetings page at http://www.nyhisto.com/annual-meetings/ ?We hope to see you there!! www.nyhsito.com ~NYSHS From ctanck1 <@t> gmail.com Sat Apr 5 11:49:37 2014 From: ctanck1 <@t> gmail.com (Carol Tanck) Date: Sat Apr 5 11:49:41 2014 Subject: [Histonet] middle school science day Message-ID: Does anyone have materials or ideas for a 20 minute presention of Histology to 4th and 5th graders? Last year we gave a short talk followed by examination of microscopic slides with microscopes. Gloves and labcoats were given to them, too! Carol Tanck carol.tanck@rochestergeneral.org From blayjorge <@t> gmail.com Sat Apr 5 19:43:47 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Sat Apr 5 19:43:53 2014 Subject: [Histonet] Looking for recommendations of listservers on genetics, incl. human genetics Message-ID: Looking for recommendations of listservers on genetics, incl. human genetics Hello Histonet-Listers: I am looking for recommendations of listservers on genetics, incl. human genetics. If you can recommend one (or more), please feel free to send me an email directly. Thank you. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From mtitford <@t> aol.com Sun Apr 6 11:58:17 2014 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Sun Apr 6 11:58:21 2014 Subject: [Histonet] Middle School Science Day Message-ID: <8D11FD08A9B3010-2850-31022@webmail-d247.sysops.aol.com> Carol Tanck asks about material for a Middle school science day. Good items to get hold of are plastinated organs that the young people can pick up and handle. If you have a medical school or community college near by, their cellular biology or anatomy departments (Titles vary in these modern times) may be able to lend you some. These organs have been thoroughly fixed, infiltrated with plastic, and then hardened. They are safe to handle and can survive a drop or two. They are especially good for showing lung tissue from smokers showing emphysema, etc. Regards Michael Titford USA Pathology Mobile AL USA From koellingr <@t> comcast.net Sun Apr 6 15:24:13 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Apr 6 15:24:41 2014 Subject: [Histonet] Middle School Science Day and OT diatribe In-Reply-To: <8D11FD08A9B3010-2850-31022@webmail-d247.sysops.aol.com> References: <8D11FD08A9B3010-2850-31022@webmail-d247.sysops.aol.com> Message-ID: <243518604.23185695.1396815853396.JavaMail.root@comcast.net> Hello everyone, thought I'd chime in here as I just returned from helping and judging at the 2-day WSSEF (Washington State Science and Engineering Fair) in Bremerton, Washington.? Had 500 incredible projects from all over the state.? It is?our state fair that leads into the ISEF, giant Intel science fair in May that draws 1,600+ students from all states and 70 countries?and territories?to compete for prestige, recognition and a million dollars of prizes and scholarship money.? So if you have little/no interest in subject heading, please save yourself a bit of reading and?just hit delete right now. ? The data is in and is incontrovertible.? The US is getting their teeth kicked in by the world when it comes to hard science and math in education and jobs.? At 2-4th grade we are above the other countries of the modern world in science and math.? At 5-7 grades, we are at around the same level as other countries and by time of graduating high school, we?are down to?25-40th place in science and math amongst those same countries depending on which educational group is testing.? So at the elementary and middle school level, I'd encourage all parents and school districts to get more STEM (science, technology, engineering, math) involved before our students start falling off the cliff in STEM education by the end of high school.? Michael Titford offers a great suggestion.? Staying within the confines of histology/pathology/lab medicine of research these are some of the things I've done in elementary and middle school school either at individual school fairs or my school-district fair.? If you can't go full blown with a microscope and such, just having a few paraffin blocks and along with those blocks a cut and stained slide and a photomicrograph, you can reveal some amazing information about lung (being lacy and full of air)?or brain or gut (being a tube) or as much as the student can follow along.? One thing that I use, rather than a microscope where only one student can look before changing, is one of those old microfiche readers that you can get for a few dollars.? Just put a slide in the plate where the microfiche went and see a rather startling enlargement.? Yes, is not 1,000x oil immersion resolution but you can see growth plates on appropriately stained bones or bronchi in lung sections or normal skin stained with a melanin stain and a skin with melanoma nodule stained with melanin stain or (fill in as many examples as you want limited only by your imagination and ingenuity).? Get a plant stem from garden, process it and cut cx and ls and see tubes or if your student is dissecting a spotted frog, get a piece of skin, fix in isopropyl alcohol for safety, process and on section can see groups of black melanin corresponding to the spots on the gross frog even if you can't see at cellular resolution.? The possibilities and permutations for showing such science to a middle-schooler are nearly limitless. And is even great in lab itself to demo faults, folds, chatter, etc.? Maybe only downside in real lab is if one of the regulators requires the "instrument" to go through QA/QC/verification etc. ? Actually at our WSSEF fair, there were several GREAT projects relating to such things as urothelial carcinoma and IL-38 and a few such?subjects and there were some histology micrographs scattered amongst those and some other projects ? Here is a quote to think about "We need to teach our kids that it's not just the winner of the Super Bowl who deserves to be celebrated but the winner of the science fair."? When talking about education and jobs now and into the future from Barack Obama in his Jan 25, 2011 State of the Union address. ? Hope everyone will support STEM, including histology, pathology, lab medicine and lab research at any science fair for any student.? Off my soap box. ? Ray, in suddenly sunny Seattle ----- Original Message ----- From: mtitford@aol.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 6, 2014 9:58:17 AM Subject: [Histonet] Middle School Science Day Carol ?Tanck asks about material for a Middle school science day. Good items to get hold of are plastinated organs that the young people can pick up and handle. If you have a medical school or community college ?near by, their cellular biology or anatomy departments ?(Titles vary in these modern times) may be able to lend you some. These organs have been thoroughly fixed, infiltrated with plastic, and then hardened. They are safe to handle and can survive a drop or two. They are especially good for showing lung tissue from smokers showing emphysema, etc. Regards Michael Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wgray19 <@t> sc.rr.com Sun Apr 6 15:49:41 2014 From: wgray19 <@t> sc.rr.com (Wanda Shotsberger Gray) Date: Sun Apr 6 15:49:49 2014 Subject: [Histonet] Permanent Microtome Knives Message-ID: <14538cdbeb8.277c.145c840c2545cc8d226fb8e47258f83d@sc.rr.com> CL Sturkey sells excellent knives and blades, and supports NSH and state societies. http://www.sturkey.com/products/permanent-knives They also will recondition knives. Wanda Shotsberger Gray Sent with AquaMail for Android http://www.aqua-mail.com From Tony.Reilly <@t> health.qld.gov.au Sun Apr 6 19:41:51 2014 From: Tony.Reilly <@t> health.qld.gov.au (Tony Reilly) Date: Sun Apr 6 19:42:31 2014 Subject: [Histonet] RE: GI Biopsies In-Reply-To: <3566D9E34287BE4B95372179009446A01C16B717@EXCHANGE.fmhnt.fmh.org> References: <3566D9E34287BE4B95372179009446A01C16B717@EXCHANGE.fmhnt.fmh.org> Message-ID: Hi Tasha Formalin buffer salts are soluble in water but not alcohol. I had a shredding problem once before when the blocks did not have adequate time in 70% Alcohol following formalin. The buffer salts precipitate inside the tissue and cause shredding at microtomy. This may not be your problem but as you are using a microwave processor and processing very rapidly it may be worth investigating. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _________________________________________________ Health Services Support Agency| Department of Health Building 15, Level 1,? 199 Ipswich Road? WOOLLOONGABBA? Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reillyi@health.qld.gov.au | www.health.qld.gov.au ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tasha M. Campbell Sent: Friday, 4 April 2014 9:58 PM To: Tony Reilly; histonet@lists.utsouthwestern.edu Subject: [Histonet] GI Biopsies Does anyone have problems with their GI biopsies kind of shredding when cutting? I microwave process them in the Shurwave microwave processor. I have been doing this for over a year and only the past couple months I have noticed that almost every biopsy I cut has knife lines in it. I have to change my blade constantly. I was told by a TBS technician that if I ever noticed the tissue doing this, it meant that I did not have all the alcohol out of the tissue, but does that sound like it would cause the tissue to shred like that? I don't use my reagents more than 2 times and I have even tried increasing the time and temp a little but no help. I have been doing them for a year and did not have a problem and I haven't changed anything. Any solutions or suggestions??? Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. 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Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From tbraud <@t> holyredeemer.com Mon Apr 7 09:21:20 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Mon Apr 7 09:21:24 2014 Subject: [Histonet] RE: Histonet Digest, Vol 125, Issue 6 In-Reply-To: <20140405161814.68B0F1E8062@trendmess-svr.holyredeemer.local> References: <20140405161814.68B0F1E8062@trendmess-svr.holyredeemer.local> Message-ID: We've used this instrument for 3 years. The techs took to it like a duck to water. We've had almost no downtime. The company is awesome; super support. I wish it had online antigen retrieval, but I know they have a newer instrument coming out that does. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. Biocare intellipath (Deloris Carter) Message: 2 Date: Fri, 4 Apr 2014 12:39:05 -0500 From: Deloris Carter Subject: [Histonet] Biocare intellipath I'm looking for some honest feedback from anyone using the Biocare Intellipath in a clinical setting. Pros, cons, etc. Thanks, Deloris Carter, HT(ASCP) delsuec@gmail.com --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From liz <@t> premierlab.com Mon Apr 7 09:23:54 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Apr 7 09:24:01 2014 Subject: [Histonet] middle school science day In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E057F3@SBS2K8.premierlab.local> Carol When we has some 5th graders in the lab. I put together a 20 minute talk on cells and tissues, it was very basic. For some reason I can't seem to locate it but if I do I'll share it. They then spent time in the lab, they printed their own cassette and embedded a slim jim, they got to watch someone section, we let them pick up the sections on the waterbath and they got to stain an H&E and iron stain. We divided them up into groups of 10 and they rotated through three modules, - 1 - the lecture 2- processing, embedding and sectioning and 3 - staining. NSH has some nice flyers that you can give to them and they also have a video on being a histotech. We asked vendors for goodies and then put together a bag that had a bunch of stuff in it for each of the students, we do this every year for both the 5th graders and then a summer class of Juniors and Seniors from Frontiers of Science it's a program at Northern Colorado University. Go to the Mich. Assoc. Of Lab. Sci. Educators (MALSE) website (program directors of MT, MLT, HT, HTL, PA, CT programs in Michigan put this together). http://www.malse.org/ On the left, lots of information to choose from Click on "Links", and it should take you to "Diagnostic Detectives" Click on "Diagnostic Detectives CD" on lower right, then click on "Click here to launch" Opens to a general CD of laboratory Can click on middle bottom for Anatomic Pathology Labs Opens to CD on Anat Path On left, can click on more CD for histology, cytology, PA Then on the left of each of these, there are panels with information about high school preparation, education training, and career ladders and certification Across the top of Diagnostic Detectives is "other" careers - vet, forensic, supervisor, etc. While on Diagnostic Detectives, check out Resources of Teachers on the left, and click on "Diagnostic Detective Toolkit", first bullet in white area. Check out Teachers Tools or Students Tools. also check out "Other Resources", and try "histology world". Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Tanck Sent: Saturday, April 05, 2014 10:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] middle school science day Does anyone have materials or ideas for a 20 minute presention of Histology to 4th and 5th graders? Last year we gave a short talk followed by examination of microscopic slides with microscopes. Gloves and labcoats were given to them, too! Carol Tanck carol.tanck@rochestergeneral.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.LaFriniere <@t> ccplab.com Mon Apr 7 12:41:33 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Mon Apr 7 12:43:15 2014 Subject: [Histonet] RE: Instrument Verification In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E057D4@SBS2K8.premierlab.local> References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> <4EE642D353925D4D96CB95E12427DBAE5C90FE@NODCMSTMBX06.no.trinity-health.org> <14E2C6176416974295479C64A11CB9AE019C79E057D4@SBS2K8.premierlab.local> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29667EFA@AHCMSASEXCH02.my.ahc.local> I agree with Elizabeth, and do exactly what she outlines and I am a routine large AP lab. What a great suggestion for classes at the State, Regional and NSH meetings! It's a great plug and well worth the mentioning Elizabeth! This is something everyone can benefit from the bench tech all the way up the ladder! Appropriate Methodologies to Validate instrumentation is a much needed topic! Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A . Silver Spring, MD 20904 P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, April 03, 2014 4:17 PM To: Martha Ward-Pathology; Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification Hello All Coming from a GLP environment this type of equipment validation is standard in our setting. This is just my opinion but I think the CAP checklist is moving towards the type of equipment documentation that is already required in a GxP or ISO environment. I always thought that instrument qualification (IQ) - operational qualification (OQ) and process qualification (PQ) or simply stated IQ/OQ/PQ were used only in GxP settings but you now see some of the larger clinical labs running these types of validations on their equipment and processes. To me it does make sense that some type of equipment validation should be required whether it is a two page document on the microtomes, waterbaths, etc. or complete IQ/OQ/PQ's on major pieces of equipment such as tissue processors, immunostainers and IHC retrieval units. I believe that all of these are important processes that should be completed in histology laboratories today. We are a GLP compliant lab and every single piece of equipment is calibrated and validated as designated in our Master Validation Plan. IHC stainers and retrieval units should be validated, even our refrigerators and freezers are calibrated and validated. Our pipettors are calibrated quarterly, and any piece of equipment that generates a weight or temperature is calibrated yearly. For example if you do not validate your IHC retrieval units how can you really tell if they reach the temperature that they are programmed to reach, does the temperature stay consistent through the retrieval process, did it retrieve for the time programmed? The only way to determine this is to perform a validation. How do you troubleshoot problems if you do not know if your instruments are performing to their specification without testing those specifications - that's what equipment validation is and that's why in my opinion its important. Histology laboratories are now responsible for running IHC that directly effects a patients treatment - meaning the numerous therapeutic and prognostic markers we routinely run now. Validation is an important process especially if you are using image analysis for these markers. I hate to say it but we better get used to it, because this is not going away. And now the shameless plug - I will be giving a 90 minute lecture at the Florida State Meeting https://classic.regonline.com/custImages/240000/241449/FSH2014OnlineProgram.pdf on this exact topic, so if you want to learn how to create a Master Validation Plan and learn how to perform a basic validation or a more detailed IQ/OQ and PQ and to what extent you need to validate a particular piece of equipment - sign up for the meeting plus there are lots of other great topics being presented too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, April 03, 2014 1:22 PM To: Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I'm with you. There really appears to be no value to this particular requirement. I would only be concerned with it if I had just purchased it, or moved it into our lab from another location. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, April 03, 2014 3:03 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I agree with you in that CAP is just looking for things to change and doesn't seem to be considering the change and decrease in staffing seen in clinical settings. Cryostat validation? Really....cut a slide after you have cleaned and pm'd the thing and go on. Good grief...I don't need any more paper and documentation on routine processes. As for tissue processors, I have 20 year old VIP's that have been running and producing specimens acceptably. I did validate them prior to being put in use but we didn't document like we do now. And I don't see the need to do it at this stage of use. We did do a very extensive validation on the Peloris we put into use last year and will going forward on new equipment. To me the daily QC of stain should provide our 'validation' of the process and include the processor. I am interested in others thoughts as well. Thanks for allowing me to rant. Cindi Robinson, HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Drive Dakota Dunes SD 57049 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, April 03, 2014 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 6. Validation of cryostat (Gloria Tharp) Message: 6 Date: Thu, 3 Apr 2014 09:59:26 -0500 From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. Gloria Tharp, BA, HTL(ASCP) ------------------------------ Message: 7 Date: Thu, 3 Apr 2014 15:26:17 +0000 From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? LeAnn Murphy Aultman Hospital Canton, Ohio ************************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Apr 7 12:45:58 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Apr 7 12:46:04 2014 Subject: [Histonet] RE: Instrument Verification In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D29667EFA@AHCMSASEXCH02.my.ahc.local> References: <20140403162131.B4EDD1E8043@trendmess-svr.holyredeemer.local> <4EE642D353925D4D96CB95E12427DBAE5C90FE@NODCMSTMBX06.no.trinity-health.org> <14E2C6176416974295479C64A11CB9AE019C79E057D4@SBS2K8.premierlab.local> <4A2A16B9707CE04E9CB6C82DC18C1D29667EFA@AHCMSASEXCH02.my.ahc.local> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05804@SBS2K8.premierlab.local> The NSH also has a teleconference this week on the same topic. NSH Webinar: The Basics of Verification and Validation Date: 10 Apr 2014 Contact Info: NSH Office: 443-535-4060 or histo@nsh.org The Basics of Verification and Validation (Quality Management Series) Presented by: Kathy Dwyer, HT(ASCP), Quest Diagnostics & Debbie Siena, HT(ASCP)QIHC, Statlab Medical Products CAP has redefined verification and validation in the 2013 CAP checklist . This webinar will discuss the new CAP definitions and how it affects the laboratory. Creating a laboratory verification and validation process will ensure accurate and reproducible results daily and during changes to methods and/or processes. A standardized verification and validation process will ensure excellent patient care and compliance with regulatory agencies. This webinar will guide the attendees on how to develop a new process for verifying and validating equipment and instrumentation. http://www.nsh.org/content/nsh-webinar-basics-verification-and-validation Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Michael LaFriniere [mailto:Michael.LaFriniere@ccplab.com] Sent: Monday, April 07, 2014 11:42 AM To: Elizabeth Chlipala; Martha Ward-Pathology; Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: Instrument Verification I agree with Elizabeth, and do exactly what she outlines and I am a routine large AP lab. What a great suggestion for classes at the State, Regional and NSH meetings! It's a great plug and well worth the mentioning Elizabeth! This is something everyone can benefit from the bench tech all the way up the ladder! Appropriate Methodologies to Validate instrumentation is a much needed topic! Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A . Silver Spring, MD 20904 P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, April 03, 2014 4:17 PM To: Martha Ward-Pathology; Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification Hello All Coming from a GLP environment this type of equipment validation is standard in our setting. This is just my opinion but I think the CAP checklist is moving towards the type of equipment documentation that is already required in a GxP or ISO environment. I always thought that instrument qualification (IQ) - operational qualification (OQ) and process qualification (PQ) or simply stated IQ/OQ/PQ were used only in GxP settings but you now see some of the larger clinical labs running these types of validations on their equipment and processes. To me it does make sense that some type of equipment validation should be required whether it is a two page document on the microtomes, waterbaths, etc. or complete IQ/OQ/PQ's on major pieces of equipment such as tissue processors, immunostainers and IHC retrieval units. I believe that all of these are important processes that should be completed in histology laboratories today. We are a GLP compliant lab and every single piece of equipment is calibrated and validated as designated in our Master Validation Plan. IHC stainers and retrieval units should be validated, even our refrigerators and freezers are calibrated and validated. Our pipettors are calibrated quarterly, and any piece of equipment that generates a weight or temperature is calibrated yearly. For example if you do not validate your IHC retrieval units how can you really tell if they reach the temperature that they are programmed to reach, does the temperature stay consistent through the retrieval process, did it retrieve for the time programmed? The only way to determine this is to perform a validation. How do you troubleshoot problems if you do not know if your instruments are performing to their specification without testing those specifications - that's what equipment validation is and that's why in my opinion its important. Histology laboratories are now responsible for running IHC that directly effects a patients treatment - meaning the numerous therapeutic and prognostic markers we routinely run now. Validation is an important process especially if you are using image analysis for these markers. I hate to say it but we better get used to it, because this is not going away. And now the shameless plug - I will be giving a 90 minute lecture at the Florida State Meeting https://classic.regonline.com/custImages/240000/241449/FSH2014OnlineProgram.pdf on this exact topic, so if you want to learn how to create a Master Validation Plan and learn how to perform a basic validation or a more detailed IQ/OQ and PQ and to what extent you need to validate a particular piece of equipment - sign up for the meeting plus there are lots of other great topics being presented too. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, April 03, 2014 1:22 PM To: Cynthia Robinson; Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I'm with you. There really appears to be no value to this particular requirement. I would only be concerned with it if I had just purchased it, or moved it into our lab from another location. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, April 03, 2014 3:03 PM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I agree with you in that CAP is just looking for things to change and doesn't seem to be considering the change and decrease in staffing seen in clinical settings. Cryostat validation? Really....cut a slide after you have cleaned and pm'd the thing and go on. Good grief...I don't need any more paper and documentation on routine processes. As for tissue processors, I have 20 year old VIP's that have been running and producing specimens acceptably. I did validate them prior to being put in use but we didn't document like we do now. And I don't see the need to do it at this stage of use. We did do a very extensive validation on the Peloris we put into use last year and will going forward on new equipment. To me the daily QC of stain should provide our 'validation' of the process and include the processor. I am interested in others thoughts as well. Thanks for allowing me to rant. Cindi Robinson, HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Drive Dakota Dunes SD 57049 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, April 03, 2014 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Instrument Verification I just received my midcycle CAP and for cryostat validation, we are planning to cut and stain a piece of frozen tonsil and have the path sign off on it. For the tissue processors, we will run a one minute test program. I hope this will fly. Is it just me, or is CAP insanely out of control with new or modified regulations and policies for AP? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 6. Validation of cryostat (Gloria Tharp) Message: 6 Date: Thu, 3 Apr 2014 09:59:26 -0500 From: "Gloria Tharp" Could anyone tell me how you are handling the new CAP ANP.23045 question on function and verification of equipment regarding a cryostat. Gloria Tharp, BA, HTL(ASCP) ------------------------------ Message: 7 Date: Thu, 3 Apr 2014 15:26:17 +0000 From: "Leann M. Murphy" How is everyone validating the tissue processor for new CAP ANP.23045 question on function and verification of equipment? LeAnn Murphy Aultman Hospital Canton, Ohio ************************ --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi <@t> nrh-ok.com Mon Apr 7 12:49:46 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Mon Apr 7 12:49:49 2014 Subject: [Histonet] Slide Imaging System Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B8220AC@MAIL.nrhnt.nrh-ok.com> Hi, Please I have a question and the question is I want to know if anyone has self-validated any whole slide imaging system for primary diagnosis? If so, is reimbursement a problem? Thanks, Banjo Adesuyi, BSMT, HT (ASCP), HTL (ASCP), QIHC (ASCP) Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From Michael.LaFriniere <@t> ccplab.com Mon Apr 7 13:03:10 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Mon Apr 7 13:03:23 2014 Subject: [Histonet] RE: ANP.22970 In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF3066311C@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF3066311C@CHIEX005.CHI.catholichealth.net> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29667F9F@AHCMSASEXCH02.my.ahc.local> Tanya Check CAP web site I am pretty sure they have the Her 2 not sure of MMR Michael Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Friday, March 28, 2014 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP.22970 Per CAP checklist ANP.22970, it lists the expected published benchmarks for ER/PR, does anyone know where to find them for Her2 or MMR? Thanks and Happy Friday!! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Mon Apr 7 13:33:32 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Mon Apr 7 13:33:39 2014 Subject: [Histonet] HISTOPALOOZA APRIL 25 - 27, 2014 UPDATE Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8465D2@EX-MLB-03.ad.georgiahealth.edu> Histopalooza is almost here! Our blocked rooms have sold out, but we have secured Southern Pine Cottages that are adjacent to the Lodge. Call 1-800-CALLAWAY and ask for Kit Reames McClung. The price will be $135/night, which is the HISTOPALOOZA rate. These aren't shabby cottages, so don't be afraid you'll be staying in a "Jason, Friday the 13th type" cottage. They have a fully furnished kitchen (not sure about the knives) bahahaha, dining and living room. There's also free Wi-Fi. Looking forward to seeing everyone at the end of the month. Bring your hockey mask-just kidding Billie Zimmerman GSH secretary From Vickroy.Jim <@t> mhsil.com Mon Apr 7 13:35:58 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Apr 7 13:36:04 2014 Subject: [Histonet] Independent Predictive Markers needing comparison with Benchmarks Message-ID: I am working with my pathology group to agree on which markers need to have a comparison with published benchmarks and interobserver variability among pathologists. Currently we compare ER, PR, and Her2. I have suggested that we need to compare ALK1, ROS1, and possible MMR IHC stains. Can anyone list any other predictive markers they are comparing to benchmarks and do you agree with the ones I've suggested? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From liz <@t> premierlab.com Mon Apr 7 13:48:17 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Apr 7 13:48:24 2014 Subject: [Histonet] NSH Awards 2014 Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0580A@SBS2K8.premierlab.local> Hello Histonet As the new NSH awards chair I wanted to remind everyone out there that the NSH has many awards and scholarships available to its members. The annual symposium/convention in Austin Texas is fast approaching and the deadline to submit nominations is June 1st . There are three categories for awards; leadership, education and advocacy. These awards offer financial incentives for continuing education along with recognizing the individuals within our profession who have demonstrated both dedication and excellence in the field of Histotechnology. To see what NSH has to offer check out the following link http://www.nsh.org/scholarships-awards and nominate yourself or a colleague for an award. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From joelleweaver <@t> hotmail.com Mon Apr 7 13:53:38 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Apr 7 13:53:41 2014 Subject: [Histonet] Independent Predictive Markers needing comparison with Benchmarks In-Reply-To: References: Message-ID: Agree, I think that list is a good start. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Vickroy.Jim@mhsil.com > To: histonet@lists.utsouthwestern.edu > Date: Mon, 7 Apr 2014 13:35:58 -0500 > Subject: [Histonet] Independent Predictive Markers needing comparison with Benchmarks > > I am working with my pathology group to agree on which markers need to have a comparison with published benchmarks and interobserver variability among pathologists. Currently we compare ER, PR, and Her2. I have suggested that we need to compare ALK1, ROS1, and possible MMR IHC stains. Can anyone list any other predictive markers they are comparing to benchmarks and do you agree with the ones I've suggested? > > Thanks > > James Vickroy BS, HT(ASCP) > > Surgical and Autopsy Pathology Technical Supervisor > Memorial Medical Center > 217-788-4046 > > > ________________________________ > This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From esarricks <@t> gmail.com Mon Apr 7 13:56:29 2014 From: esarricks <@t> gmail.com (Erin Sarricks) Date: Mon Apr 7 13:56:34 2014 Subject: [Histonet] Leica IP S Slide Printer Message-ID: Hi Histonet, I was wondering if anyone else has problems with their Leica IP S slide printer. We are having issues with incomplete and inconsistent printing. We clean the machine daily before use and have even tried not cleaning it to see if that would help! We also change the ink cartridge every 3 months per Leica recommendation. I'd love to hear from other people who use this machine. Thanks in advance for your help! Regards, Erin Galati, HT (ASCP) From blayjorge <@t> gmail.com Mon Apr 7 15:41:55 2014 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Mon Apr 7 15:42:00 2014 Subject: [Histonet] Is it OK to place Karnovsky's with no cacodylate buffer be placed in the freezer to extend its life? Message-ID: Hello Histonet-Listers: Is it OK to place Karnovsky's with no cacodylate buffer be placed in the freezer to extend its life? It is for fixing insect wings for TEM work. If you know, please kindly send me an email (blayjorge@gmail.com). Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.htmlHello Histonet-Listers: From SSCALISE <@t> beaumont.edu Mon Apr 7 16:52:38 2014 From: SSCALISE <@t> beaumont.edu (Sharon Scalise) Date: Mon Apr 7 16:52:43 2014 Subject: [Histonet] Transporting blocks/slides between sites Message-ID: <190D98228ADC1747BCE27019B78FD8F3011E4102@EXMAIL06.ms.beaumont.edu> We are looking for a better way to transport blocks and slides between our hospital sites. We want the system to be secure so that as we track from site to site we can be assured that the contents have not been tampered with. We currently are using plastic totes that have lids the can be secured using a zip tie. The problem is that during transport (over time) these totes get pretty beat up and end up with cracks and holes in them. We feel there is probably a better system so I am looking to the experience of the histonet users for suggestions. Thank you! Sharon Scalise, HTL(ASCP) Histology Supervisor-Anatomic Pathology Beaumont Health System 3601 W. 13 Mile Rd. Royal Oak, MI 48073 248 898-5981 sscalise@beaumont.edu From Timothy.Morken <@t> ucsfmedctr.org Mon Apr 7 17:06:00 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Mon Apr 7 17:07:01 2014 Subject: [Histonet] RE: Transporting blocks/slides between sites In-Reply-To: <190D98228ADC1747BCE27019B78FD8F3011E4102@EXMAIL06.ms.beaumont.edu> References: <190D98228ADC1747BCE27019B78FD8F3011E4102@EXMAIL06.ms.beaumont.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF36777FA6@ex07.net.ucsf.edu> Sharon, We transport slide flats and tissue containers/cassettes baskets for processing (most grossing at one site, all tissue processing at another site). We use Colman 50QT coolers with handles and wheels to transport. They take a beating. We have about 10 of these because we have them receiving material at each of 3 sites and then four being transported at all times (30 min pickup schedule, each direction). It is a cheap and effective solution. We use half for paper/slide only (clean set) and half for tissue samples. If you have less material, then maybe a smaller version of these would work for you. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Monday, April 07, 2014 2:53 PM To: 'histonet' (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Transporting blocks/slides between sites We are looking for a better way to transport blocks and slides between our hospital sites. We want the system to be secure so that as we track from site to site we can be assured that the contents have not been tampered with. We currently are using plastic totes that have lids the can be secured using a zip tie. The problem is that during transport (over time) these totes get pretty beat up and end up with cracks and holes in them. We feel there is probably a better system so I am looking to the experience of the histonet users for suggestions. Thank you! Sharon Scalise, HTL(ASCP) Histology Supervisor-Anatomic Pathology Beaumont Health System 3601 W. 13 Mile Rd. Royal Oak, MI 48073 248 898-5981 sscalise@beaumont.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ttruscot <@t> vetmed.wsu.edu Mon Apr 7 18:06:24 2014 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Mon Apr 7 18:06:30 2014 Subject: [Histonet] RE: Transporting blocks/slides between sites In-Reply-To: <761E2B5697F795489C8710BCC72141FF36777FA6@ex07.net.ucsf.edu> References: <190D98228ADC1747BCE27019B78FD8F3011E4102@EXMAIL06.ms.beaumont.edu> <761E2B5697F795489C8710BCC72141FF36777FA6@ex07.net.ucsf.edu> Message-ID: <9EF5279EBDFE6E4FB6605E8F183A00278BCBDE2C@CVM76.vetmed.wsu.edu> Hi Sharon, If you are trying to be secure enough against tampering that it would stand up in court- the zip tie method probably isn't enough. I would try to get a used small set of the larger locking safe deposit boxes or maybe a group security box from grainger. Tom T -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, April 07, 2014 3:06 PM To: 'Sharon Scalise' Cc: Histonet Subject: [Histonet] RE: Transporting blocks/slides between sites Sharon, We transport slide flats and tissue containers/cassettes baskets for processing (most grossing at one site, all tissue processing at another site). We use Colman 50QT coolers with handles and wheels to transport. They take a beating. We have about 10 of these because we have them receiving material at each of 3 sites and then four being transported at all times (30 min pickup schedule, each direction). It is a cheap and effective solution. We use half for paper/slide only (clean set) and half for tissue samples. If you have less material, then maybe a smaller version of these would work for you. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Monday, April 07, 2014 2:53 PM To: 'histonet' (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Transporting blocks/slides between sites We are looking for a better way to transport blocks and slides between our hospital sites. We want the system to be secure so that as we track from site to site we can be assured that the contents have not been tampered with. We currently are using plastic totes that have lids the can be secured using a zip tie. The problem is that during transport (over time) these totes get pretty beat up and end up with cracks and holes in them. We feel there is probably a better system so I am looking to the experience of the histonet users for suggestions. Thank you! Sharon Scalise, HTL(ASCP) Histology Supervisor-Anatomic Pathology Beaumont Health System 3601 W. 13 Mile Rd. Royal Oak, MI 48073 248 898-5981 sscalise@beaumont.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drmtech09 <@t> gmail.com Mon Apr 7 19:29:37 2014 From: drmtech09 <@t> gmail.com (Eric Baltazar) Date: Mon Apr 7 19:29:40 2014 Subject: [Histonet] IEC-CTD Cryostat Message-ID: Hello fellow Histonetters! Our company is currently looking to purchase any available IEC-CTD model cryostat machine. In case you're not familiar with how they look, they're like a mini-fridge shape size machine used awhile back for frozen section. Check your office storage areas as you may still have this just parked and sitting in your storage areas. If you do find one, pls call me ASAP or reply through this email add, as I may turn that sleeping machine into $$$$$$! Also, we're very much willing to purchase it regardless of its condition. Thank you and looking forward to a favorable reply soon! -- Eric T. Baltazar Dermtech Mohs Services Group Inc. Ofc: (510) 545 -MOHS Cell: (213) 290 -3679 From litepath2000 <@t> yahoo.com Tue Apr 8 14:00:59 2014 From: litepath2000 <@t> yahoo.com (NYSHisto) Date: Tue Apr 8 14:01:04 2014 Subject: [Histonet] CD274/PDL1 Message-ID: <1396983659.78846.YahooMailNeo@web140302.mail.bf1.yahoo.com> Can anyone recommend an antibody for IHC in human FFPE tissues? Thanks Luis From Ronald.Houston <@t> nationwidechildrens.org Tue Apr 8 14:37:00 2014 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Apr 8 14:37:28 2014 Subject: [Histonet] competency form Message-ID: Can someone please share the competency form(s) they are using to satisfy CAP? I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From amber.mckenzie <@t> gastrodocs.net Tue Apr 8 16:17:44 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Apr 8 16:17:54 2014 Subject: [Histonet] RE: competency form In-Reply-To: References: Message-ID: <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com> Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. Thanks, Amber -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, April 08, 2014 2:37 PM To: Histonet Subject: [Histonet] competency form Can someone please share the competency form(s) they are using to satisfy CAP? I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Cnituda <@t> nvdermatology.com Tue Apr 8 16:34:39 2014 From: Cnituda <@t> nvdermatology.com (Carl Nituda) Date: Tue Apr 8 16:34:54 2014 Subject: [Histonet] SB1215 for inoffice labs Message-ID: Any comments about SB 1215 for inoffice labs? Regards, Carl -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, April 08, 2014 2:18 PM To: Houston, Ronald; Histonet Subject: [Histonet] RE: competency form Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. Thanks, Amber -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, April 08, 2014 2:37 PM To: Histonet Subject: [Histonet] competency form Can someone please share the competency form(s) they are using to satisfy CAP? I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsfmedctr.org Tue Apr 8 17:07:21 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Apr 8 17:07:34 2014 Subject: [Histonet] Now HERE's an embedding tamper! Message-ID: <761E2B5697F795489C8710BCC72141FF36778585@ex07.net.ucsf.edu> A while ago I posted asking about embedding tampers for Mega molds (whole mount prostate for the most part). There were a few ideas, but not what I was looking for. Finally I found it. Here is an embedding tamper made from an espresso coffee tamper. Stainless steel. Large. Heavy. Fantastic. http://i1248.photobucket.com/albums/hh491/tmorken/tamper3_zpscbd10f47.jpg http://i1248.photobucket.com/albums/hh491/tmorken/tamper2_zps114d76ae.jpg http://i1248.photobucket.com/albums/hh491/tmorken/Tamper1_zpse28869cf.jpg Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org From joelleweaver <@t> hotmail.com Tue Apr 8 17:18:44 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Apr 8 17:18:49 2014 Subject: [Histonet] Now HERE's an embedding tamper! In-Reply-To: <761E2B5697F795489C8710BCC72141FF36778585@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF36778585@ex07.net.ucsf.edu> Message-ID: Wow Tim, you are right- that is quite a tamper! Congratulations on your find. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken@ucsfmedctr.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 8 Apr 2014 22:07:21 +0000 > Subject: [Histonet] Now HERE's an embedding tamper! > > A while ago I posted asking about embedding tampers for Mega molds (whole mount prostate for the most part). There were a few ideas, but not what I was looking for. > > Finally I found it. > > Here is an embedding tamper made from an espresso coffee tamper. Stainless steel. Large. Heavy. Fantastic. > > http://i1248.photobucket.com/albums/hh491/tmorken/tamper3_zpscbd10f47.jpg > > http://i1248.photobucket.com/albums/hh491/tmorken/tamper2_zps114d76ae.jpg > > http://i1248.photobucket.com/albums/hh491/tmorken/Tamper1_zpse28869cf.jpg > > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > Box 1656 > 505 Parnassus Ave > San Francisco, CA 94143 > USA > > 415.514-6042 (office) > tim.morken@ucsfmedctr.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Apr 8 21:03:01 2014 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Apr 8 21:03:05 2014 Subject: [Histonet] Leica IPs slide printer Message-ID: Hi, Leica instruments are usuly over-engineered finickey pains in the neck that work fantastic when they want to but really want to try to fly out the window with troubling regularity. Our IPs has been babied for a long time. The ink on these tends to get really gummy after a while. You are doing it a great service by cleaning it and changing the ink regularly. The problem you are likely having is in the path between the ink and the nozzle. Mike Saia from Belair showed me an awesome trick. It is messy though. Take an old print cartridge and cut a hole in the top. Dump out the ink and clean the hell out of it with ethanol. Then fill the cartridge with fresh 100% ethanol and switch this out with the current cartridge. Prime the pump several times until the black tube in the front clears so you can see fluid passing through it. Then swap out the cartridge again and prime the pump again. It should clean the line. We recently also had our pump die on us. All the cleaning in the world wouldnt have helped. This needed a service call to fix. Belair has been fantastic. If you can get Mike, he is a wiz with these things. Best of luck, Amos ------------------------------ Message: 9 Date: Mon, 7 Apr 2014 11:56:29 -0700 From: Erin Sarricks Subject: [Histonet] Leica IP S Slide Printer To: histonet Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi Histonet, I was wondering if anyone else has problems with their Leica IP S slide printer. We are having issues with incomplete and inconsistent printing. We clean the machine daily before use and have even tried not cleaning it to see if that would help! We also change the ink cartridge every 3 months per Leica recommendation. I'd love to hear from other people who use this machine. Thanks in advance for your help! Regards, Erin Galati, HT (ASCP From smah2 <@t> msn.com Wed Apr 9 06:10:18 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Wed Apr 9 06:10:29 2014 Subject: [Histonet] RE: competency form In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com> References: , <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com> Message-ID: We were recently dinged by CAP for our competency assessments in all areas. While that's no longer my immediate responsibility, I would love to be able to assist the lab manager with some information so we can tweek our assessments if you all wouldn't mind sharing with me as well. Thanks a bunch. Sheila Haas MicroPath Laboratories, Inc. Quality Assurance Coordinator > From: amber.mckenzie@gastrodocs.net > To: Ronald.Houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > Date: Tue, 8 Apr 2014 21:17:44 +0000 > CC: > Subject: [Histonet] RE: competency form > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > Thanks, > Amber > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > Sent: Tuesday, April 08, 2014 2:37 PM > To: Histonet > Subject: [Histonet] competency form > > Can someone please share the competency form(s) they are using to satisfy CAP? > > I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results > > Thanks > > Ronnie Houston, MS HT(ASCP)QIHC > Anatomic Pathology Manager > ChildLab, a Division of Nationwide Children's Hospital www.childlab.com > > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.houston@nationwidechildrens.org > www.NationwideChildrens.org > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sadrew <@t> wisc.edu Wed Apr 9 07:19:30 2014 From: sadrew <@t> wisc.edu (Sally Ann Drew) Date: Wed Apr 9 07:19:27 2014 Subject: [Histonet] RE: competency form In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com> Message-ID: <002401cf53ed$f57c6ac0$e0754040$@wisc.edu> I don't know if it's reasonable to share with all of us, but I bet there are a lot of us that would be interested in how others are approaching these topics. I doubt there are that many workshops on the issue-or have there been teleconferences maybe that cover the subject? _Sally Sally Ann Drew,MT(ASCP) UW SMPH-Dept. of Pathology TRIP/TSB-TRIP Lab Manager CSC, K4/435 608.265.4378 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, April 08, 2014 4:18 PM To: Houston, Ronald; Histonet Subject: [Histonet] RE: competency form Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. Thanks, Amber -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, April 08, 2014 2:37 PM To: Histonet Subject: [Histonet] competency form Can someone please share the competency form(s) they are using to satisfy CAP? I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl <@t> gmail.com Wed Apr 9 07:22:34 2014 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Wed Apr 9 07:22:38 2014 Subject: [Histonet] RE: competency form In-Reply-To: <002401cf53ed$f57c6ac0$e0754040$@wisc.edu> References: <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com> <002401cf53ed$f57c6ac0$e0754040$@wisc.edu> Message-ID: We based ours off the Michigan Society of Histotechnologist's Competency Assessment Handbook (available on their website for a nominal fee). We took some of their forms/ideas and modified them for our use. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Wed, Apr 9, 2014 at 8:19 AM, Sally Ann Drew wrote: > I don't know if it's reasonable to share with all of us, but I bet there > are > a lot of us that would be interested in how others are approaching these > topics. I doubt there are that many workshops on the issue-or have there > been teleconferences maybe that cover the subject? > > _Sally > > Sally Ann Drew,MT(ASCP) > UW SMPH-Dept. of Pathology > TRIP/TSB-TRIP Lab Manager > CSC, K4/435 > 608.265.4378 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > McKenzie > Sent: Tuesday, April 08, 2014 4:18 PM > To: Houston, Ronald; Histonet > Subject: [Histonet] RE: competency form > > Can you pass it on to me as well? I'd love to compare what I've got to > what > someone else is doing. > Thanks, > Amber > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, > Ronald > Sent: Tuesday, April 08, 2014 2:37 PM > To: Histonet > Subject: [Histonet] competency form > > Can someone please share the competency form(s) they are using to satisfy > CAP? > > I am having problems convincing our QA/Compliance folks of the differences > between testing in AP compared to the other lab disciplines, who do read > our > test results > > Thanks > > Ronnie Houston, MS HT(ASCP)QIHC > Anatomic Pathology Manager > ChildLab, a Division of Nationwide Children's Hospital www.childlab.com > > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.houston@nationwidechildrens.org ronald.houston@nationwidechild > rens.org> > www.NationwideChildrens.org > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From smah2 <@t> msn.com Wed Apr 9 07:24:36 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Wed Apr 9 07:24:40 2014 Subject: [Histonet] RE: competency form In-Reply-To: References: , <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com>, <002401cf53ed$f57c6ac0$e0754040$@wisc.edu>, Message-ID: Thanks for the reference Patrick! > Date: Wed, 9 Apr 2014 08:22:34 -0400 > From: foreightl@gmail.com > To: sadrew@wisc.edu > Subject: Re: [Histonet] RE: competency form > CC: histonet@lists.utsouthwestern.edu; Ronald.Houston@nationwidechildrens.org > > We based ours off the Michigan Society of Histotechnologist's Competency > Assessment Handbook (available on their website for a nominal fee). We > took some of their forms/ideas and modified them for our use. > > Patrick Laurie(HT)ASCP QIHC > > Histology Manager > > Celligent Diagnostics, LLC > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > Work: 704-970-3300 Cell: 704-266-0869 > > > On Wed, Apr 9, 2014 at 8:19 AM, Sally Ann Drew wrote: > > > I don't know if it's reasonable to share with all of us, but I bet there > > are > > a lot of us that would be interested in how others are approaching these > > topics. I doubt there are that many workshops on the issue-or have there > > been teleconferences maybe that cover the subject? > > > > _Sally > > > > Sally Ann Drew,MT(ASCP) > > UW SMPH-Dept. of Pathology > > TRIP/TSB-TRIP Lab Manager > > CSC, K4/435 > > 608.265.4378 > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > > McKenzie > > Sent: Tuesday, April 08, 2014 4:18 PM > > To: Houston, Ronald; Histonet > > Subject: [Histonet] RE: competency form > > > > Can you pass it on to me as well? I'd love to compare what I've got to > > what > > someone else is doing. > > Thanks, > > Amber > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, > > Ronald > > Sent: Tuesday, April 08, 2014 2:37 PM > > To: Histonet > > Subject: [Histonet] competency form > > > > Can someone please share the competency form(s) they are using to satisfy > > CAP? > > > > I am having problems convincing our QA/Compliance folks of the differences > > between testing in AP compared to the other lab disciplines, who do read > > our > > test results > > > > Thanks > > > > Ronnie Houston, MS HT(ASCP)QIHC > > Anatomic Pathology Manager > > ChildLab, a Division of Nationwide Children's Hospital www.childlab.com > > > > 700 Children's Drive > > Columbus, OH 43205 > > (P) 614-722-5450 > > (F) 614-722-2899 > > ronald.houston@nationwidechildrens.org > ronald.houston@nationwidechild > > rens.org> > > www.NationwideChildrens.org > > > > "One person with passion is better than forty people merely interested." > > ~ E.M. Forster > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Apr 9 07:26:47 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Apr 9 07:26:52 2014 Subject: [Histonet] RE: competency form In-Reply-To: References: , , <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com>, Message-ID: Is the question that Ronnie posed yesterday requesting justification of the need and extent of competency documentation for histology, or is it just a form needed? The general checklist pretty much sums up the necessity for doing, and required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding? As for a form, I would expect that the specific items on any forms will vary by your personnel and by the testing and processes you perform. What I did to document initial training and competency was a make a summary checklist for each "bench" with tasks and direct observations "DO" for initial training & documentation of satisfactory performance before patient testing. I just put all those checklists together in a summary table for each person. High complexity; such as grossing, IHC, FISH scoring get more attention and documentation, the waived tests, you have more discretion,- but I thought it easier to do everything about the same. I have not been inspected on this document yet ( so can't say if CAP will have issues with it- but will know soon...) but here is basically what I did to meet GEN.55500 or the main parts; Defined how competency is monitored- method and frequency ( just included as part of the competency SOP) Orientation and initial training documentation in a checklist for general lab, safety Training checklist on each technical bench, instrument, major procedure PT records and performance/results DO- a practical assessment ( block, slides, stains), for the assessment of previously analyzed specimens, and a PI feedback checklist for the technical from this audit of issues- how/what to improve Check off in performing QC, calibration, patient ID procedures ( acceptable error rates), examples for file DO of grossing, other performance such as instrument programming/maintenance Written quiz, policies & procedures, troubleshooting( problem solving documentation) Continuing education participation records Joelle Weaver MAOM, HTL (ASCP) QIHC > From: smah2@msn.com > To: amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > Date: Wed, 9 Apr 2014 11:10:18 +0000 > Subject: RE: [Histonet] RE: competency form > CC: > > We were recently dinged by CAP for our competency assessments in all areas. While that's no longer my immediate responsibility, I would love to be able to assist the lab manager with some information so we can tweek our assessments if you all wouldn't mind sharing with me as well. > Thanks a bunch. > Sheila Haas > MicroPath Laboratories, Inc. > Quality Assurance Coordinator > > > From: amber.mckenzie@gastrodocs.net > > To: Ronald.Houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > > Date: Tue, 8 Apr 2014 21:17:44 +0000 > > CC: > > Subject: [Histonet] RE: competency form > > > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > > Thanks, > > Amber > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > > Sent: Tuesday, April 08, 2014 2:37 PM > > To: Histonet > > Subject: [Histonet] competency form > > > > Can someone please share the competency form(s) they are using to satisfy CAP? > > > > I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results > > > > Thanks > > > > Ronnie Houston, MS HT(ASCP)QIHC > > Anatomic Pathology Manager > > ChildLab, a Division of Nationwide Children's Hospital www.childlab.com > > > > 700 Children's Drive > > Columbus, OH 43205 > > (P) 614-722-5450 > > (F) 614-722-2899 > > ronald.houston@nationwidechildrens.org > > www.NationwideChildrens.org > > > > "One person with passion is better than forty people merely interested." > > ~ E.M. Forster > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Apr 9 07:33:29 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Apr 9 07:33:38 2014 Subject: [Histonet] RE: competency form In-Reply-To: References: , , <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com>, , <002401cf53ed$f57c6ac0$e0754040$@wisc.edu>, , , Message-ID: The NSH does have some good basic forms that may help in getting your organization's forms together. I would probably evolve them from those into what fits in with your situation. There have been a couple of webinars in the past dealing with competency in histology and meeting requirements under CLIA and CAP. I think that it is a recurring topic that comes up since competency crosses over into many other arenas such as personnel & staffing, education & training, compliance, test performance( reliability/reproducibility), errors and process improvements, and potentially other areas that supervisors and managers juggle every day. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: smah2@msn.com > To: foreightl@gmail.com; sadrew@wisc.edu > Date: Wed, 9 Apr 2014 12:24:36 +0000 > Subject: RE: [Histonet] RE: competency form > CC: histonet@lists.utsouthwestern.edu; ronald.houston@nationwidechildrens.org > > Thanks for the reference Patrick! > > > > Date: Wed, 9 Apr 2014 08:22:34 -0400 > > From: foreightl@gmail.com > > To: sadrew@wisc.edu > > Subject: Re: [Histonet] RE: competency form > > CC: histonet@lists.utsouthwestern.edu; Ronald.Houston@nationwidechildrens.org > > > > We based ours off the Michigan Society of Histotechnologist's Competency > > Assessment Handbook (available on their website for a nominal fee). We > > took some of their forms/ideas and modified them for our use. > > > > Patrick Laurie(HT)ASCP QIHC > > > > Histology Manager > > > > Celligent Diagnostics, LLC > > > > 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 > > > > Work: 704-970-3300 Cell: 704-266-0869 > > > > > > On Wed, Apr 9, 2014 at 8:19 AM, Sally Ann Drew wrote: > > > > > I don't know if it's reasonable to share with all of us, but I bet there > > > are > > > a lot of us that would be interested in how others are approaching these > > > topics. I doubt there are that many workshops on the issue-or have there > > > been teleconferences maybe that cover the subject? > > > > > > _Sally > > > > > > Sally Ann Drew,MT(ASCP) > > > UW SMPH-Dept. of Pathology > > > TRIP/TSB-TRIP Lab Manager > > > CSC, K4/435 > > > 608.265.4378 > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber > > > McKenzie > > > Sent: Tuesday, April 08, 2014 4:18 PM > > > To: Houston, Ronald; Histonet > > > Subject: [Histonet] RE: competency form > > > > > > Can you pass it on to me as well? I'd love to compare what I've got to > > > what > > > someone else is doing. > > > Thanks, > > > Amber > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, > > > Ronald > > > Sent: Tuesday, April 08, 2014 2:37 PM > > > To: Histonet > > > Subject: [Histonet] competency form > > > > > > Can someone please share the competency form(s) they are using to satisfy > > > CAP? > > > > > > I am having problems convincing our QA/Compliance folks of the differences > > > between testing in AP compared to the other lab disciplines, who do read > > > our > > > test results > > > > > > Thanks > > > > > > Ronnie Houston, MS HT(ASCP)QIHC > > > Anatomic Pathology Manager > > > ChildLab, a Division of Nationwide Children's Hospital www.childlab.com > > > > > > 700 Children's Drive > > > Columbus, OH 43205 > > > (P) 614-722-5450 > > > (F) 614-722-2899 > > > ronald.houston@nationwidechildrens.org > > ronald.houston@nationwidechild > > > rens.org> > > > www.NationwideChildrens.org > > > > > > "One person with passion is better than forty people merely interested." > > > ~ E.M. Forster > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smah2 <@t> msn.com Wed Apr 9 07:51:46 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Wed Apr 9 07:51:51 2014 Subject: [Histonet] RE: competency form In-Reply-To: References: , , <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com>, , Message-ID: What we had here, which did not meet the CAP inspectors requirements apparently, were the procedure (of course); a form with each observation of each task documented along with any corrective action necessary; the correlation of proficiency tests, educational assessments and performance reviews for technical staff; daily evaluations from the pathologists concerning staining, microtomy and grossing; and educational training documentation. We had no idea with all pieces of this documentation that we were anticipated to have more. The form for DO of each task was not detailed enough (despite listing each task and proficiency or corrective action of each task) according to the inspectors. I was hoping someone could share a form so as to assist us in seeing what holes there are in our form. While this DO form is definitely not our only form, the inspectors specifically commented on this one. If anyone can assist, it would be helpful. Thank you, Sheila Haas MicroPath Laboratories, Inc. Quality Assurance Coordinator From: joelleweaver@hotmail.com To: smah2@msn.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: competency form Date: Wed, 9 Apr 2014 12:26:47 +0000 Is the question that Ronnie posed yesterday requesting justification of the need and extent of competency documentation for histology, or is it just a form needed? The general checklist pretty much sums up the necessity for doing, and required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding? As for a form, I would expect that the specific items on any forms will vary by your personnel and by the testing and processes you perform. What I did to document initial training and competency was a make a summary checklist for each "bench" with tasks and direct observations "DO" for initial training & documentation of satisfactory performance before patient testing. I just put all those checklists together in a summary table for each person. High complexity; such as grossing, IHC, FISH scoring get more attention and documentation, the waived tests, you have more discretion,- but I thought it easier to do everything about the same. I have not been inspected on this document yet ( so can't say if CAP will have issues with it- but will know soon...) but here is basically what I did to meet GEN.55500 or the main parts; Defined how competency is monitored- method and frequency ( just included as part of the competency SOP) Orientation and initial training documentation in a checklist for general lab, safety Training checklist on each technical bench, instrument, major procedure PT records and performance/results DO- a practical assessment ( block, slides, stains), for the assessment of previously analyzed specimens, and a PI feedback checklist for the technical from this audit of issues- how/what to improve Check off in performing QC, calibration, patient ID procedures ( acceptable error rates), examples for file DO of grossing, other performance such as instrument programming/maintenance Written quiz, policies & procedures, troubleshooting( problem solving documentation) Continuing education participation records Joelle Weaver MAOM, HTL (ASCP) QIHC > From: smah2@msn.com > To: amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > Date: Wed, 9 Apr 2014 11:10:18 +0000 > Subject: RE: [Histonet] RE: competency form > CC: > > We were recently dinged by CAP for our competency assessments in all areas. While that's no longer my immediate responsibility, I would love to be able to assist the lab manager with some information so we can tweek our assessments if you all wouldn't mind sharing with me as well. > Thanks a bunch. > Sheila Haas > MicroPath Laboratories, Inc. > Quality Assurance Coordinator > > > From: amber.mckenzie@gastrodocs.net > > To: Ronald.Houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > > Date: Tue, 8 Apr 2014 21:17:44 +0000 > > CC: > > Subject: [Histonet] RE: competency form > > > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > > Thanks, > > Amber > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > > Sent: Tuesday, April 08, 2014 2:37 PM > > To: Histonet > > Subject: [Histonet] competency form > > > > Can someone please share the competency form(s) they are using to satisfy CAP? > > > > I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results > > > > Thanks > > > > Ronnie Houston, MS HT(ASCP)QIHC > > Anatomic Pathology Manager > > ChildLab, a Division of Nationwide Children's Hospital www.childlab.com > > > > 700 Children's Drive > > Columbus, OH 43205 > > (P) 614-722-5450 > > (F) 614-722-2899 > > ronald.houston@nationwidechildrens.org > > www.NationwideChildrens.org > > > > "One person with passion is better than forty people merely interested." > > ~ E.M. Forster > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Wed Apr 9 08:04:17 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Apr 9 08:05:41 2014 Subject: [Histonet] RE: competency form In-Reply-To: References: , , <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com>, , Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95C7064@SMCMAIL01.somerset-healthcare.com> CAP inspectors may have opinions which differ from our own, and their interpretation of standards may also be different. Have you challenged this deficiency with CAP? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 09, 2014 8:52 AM To: joelle weaver; Amber McKenzie; Houston, Ronald; Histonet Subject: RE: [Histonet] RE: competency form What we had here, which did not meet the CAP inspectors requirements apparently, were the procedure (of course); a form with each observation of each task documented along with any corrective action necessary; the correlation of proficiency tests, educational assessments and performance reviews for technical staff; daily evaluations from the pathologists concerning staining, microtomy and grossing; and educational training documentation. We had no idea with all pieces of this documentation that we were anticipated to have more. The form for DO of each task was not detailed enough (despite listing each task and proficiency or corrective action of each task) according to the inspectors. I was hoping someone could share a form so as to assist us in seeing what holes there are in our form. While this DO form is definitely not our only form, the inspectors specifically commented on this one. If anyone can assist, it would be helpful. Thank you, Sheila Haas MicroPath Laboratories, Inc. Quality Assurance Coordinator From: joelleweaver@hotmail.com To: smah2@msn.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: competency form Date: Wed, 9 Apr 2014 12:26:47 +0000 Is the question that Ronnie posed yesterday requesting justification of the need and extent of competency documentation for histology, or is it just a form needed? The general checklist pretty much sums up the necessity for doing, and required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding? As for a form, I would expect that the specific items on any forms will vary by your personnel and by the testing and processes you perform. What I did to document initial training and competency was a make a summary checklist for each "bench" with tasks and direct observations "DO" for initial training & documentation of satisfactory performance before patient testing. I just put all those checklists together in a summary table for each person. High complexity; such as grossing, IHC, FISH scoring get more attention and documentation, the waived tests, you have more discretion,- but I thought it easier to do everything about the same. I have not been inspected on this document yet ( so can't say if CAP will have issues with it- but will know soon...) but here is basically what I did to meet GEN.55500 or the main parts; Defined how competency is monitored- method and frequency ( just included as part of the competency SOP) Orientation and initial training documentation in a checklist for general lab, safety Training checklist on each technical bench, instrument, major procedure PT records and performance/results DO- a practical assessment ( block, slides, stains), for the assessment of previously analyzed specimens, and a PI feedback checklist for the technical from this audit of issues- how/what to improve Check off in performing QC, calibration, patient ID procedures ( acceptable error rates), examples for file DO of grossing, other performance such as instrument programming/maintenance Written quiz, policies & procedures, troubleshooting( problem solving documentation) Continuing education participation records Joelle Weaver MAOM, HTL (ASCP) QIHC > From: smah2@msn.com > To: amber.mckenzie@gastrodocs.net; > ronald.houston@nationwidechildrens.org; > histonet@lists.utsouthwestern.edu > Date: Wed, 9 Apr 2014 11:10:18 +0000 > Subject: RE: [Histonet] RE: competency form > CC: > > We were recently dinged by CAP for our competency assessments in all areas. While that's no longer my immediate responsibility, I would love to be able to assist the lab manager with some information so we can tweek our assessments if you all wouldn't mind sharing with me as well. > Thanks a bunch. > Sheila Haas > MicroPath Laboratories, Inc. > Quality Assurance Coordinator > > > From: amber.mckenzie@gastrodocs.net > > To: Ronald.Houston@nationwidechildrens.org; > > histonet@lists.utsouthwestern.edu > > Date: Tue, 8 Apr 2014 21:17:44 +0000 > > CC: > > Subject: [Histonet] RE: competency form > > > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > > Thanks, > > Amber > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > Houston, Ronald > > Sent: Tuesday, April 08, 2014 2:37 PM > > To: Histonet > > Subject: [Histonet] competency form > > > > Can someone please share the competency form(s) they are using to satisfy CAP? > > > > I am having problems convincing our QA/Compliance folks of the > > differences between testing in AP compared to the other lab > > disciplines, who do read our test results > > > > Thanks > > > > Ronnie Houston, MS HT(ASCP)QIHC > > Anatomic Pathology Manager > > ChildLab, a Division of Nationwide Children's Hospital > > www.childlab.com > > > > 700 Children's Drive > > Columbus, OH 43205 > > (P) 614-722-5450 > > (F) 614-722-2899 > > ronald.houston@nationwidechildrens.org > idechildrens.org> > > www.NationwideChildrens.org > > > > "One person with passion is better than forty people merely interested." > > ~ E.M. Forster > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Wed Apr 9 08:09:00 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Apr 9 08:09:05 2014 Subject: [Histonet] RE: competency form In-Reply-To: <3AD061FE740D464FAC7BF6B5CFB75707A95C7064@SMCMAIL01.somerset-healthcare.com> References: , , , <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com>, , , , , , <3AD061FE740D464FAC7BF6B5CFB75707A95C7064@SMCMAIL01.somerset-healthcare.com> Message-ID: True there is inspector variation. Maybe we should all pool our resources and see what we can come up with collaboratively? It is great to know what the CAP feedback has been so we can all fill in any gaps. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: trathborne@somerset-healthcare.com > To: smah2@msn.com; joelleweaver@hotmail.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: competency form > Date: Wed, 9 Apr 2014 13:04:17 +0000 > > CAP inspectors may have opinions which differ from our own, and their interpretation of standards may also be different. Have you challenged this deficiency with CAP? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Wednesday, April 09, 2014 8:52 AM > To: joelle weaver; Amber McKenzie; Houston, Ronald; Histonet > Subject: RE: [Histonet] RE: competency form > > What we had here, which did not meet the CAP inspectors requirements apparently, were the procedure (of course); a form with each observation of each task documented along with any corrective action necessary; the correlation of proficiency tests, educational assessments and performance reviews for technical staff; daily evaluations from the pathologists concerning staining, microtomy and grossing; and educational training documentation. We had no idea with all pieces of this documentation that we were anticipated to have more. The form for DO of each task was not detailed enough (despite listing each task and proficiency or corrective action of each task) according to the inspectors. I was hoping someone could share a form so as to assist us in seeing what holes there are in our form. While this DO form is definitely not our only form, the inspectors specifically commented on this one. > > If anyone can assist, it would be helpful. > Thank you, > Sheila Haas > MicroPath Laboratories, Inc. > Quality Assurance Coordinator > > > From: joelleweaver@hotmail.com > To: smah2@msn.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: competency form > Date: Wed, 9 Apr 2014 12:26:47 +0000 > > > > > > Is the question that Ronnie posed yesterday requesting justification of the need and extent of competency documentation for histology, or is it just a form needed? The general checklist pretty much sums up the necessity for doing, and required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding? > As for a form, I would expect that the specific items on any forms will vary by your personnel and by the testing and processes you perform. > What I did to document initial training and competency was a make a summary checklist for each "bench" with tasks and direct observations "DO" for initial training & documentation of satisfactory performance before patient testing. > I just put all those checklists together in a summary table for each person. High complexity; such as grossing, IHC, FISH scoring get more attention and documentation, the waived tests, you have more discretion,- but I thought it easier to do everything about the same. I have not been inspected on this document yet ( so can't say if CAP will have issues with it- but will know soon...) but here is basically what I did to meet GEN.55500 or the main parts; > > > Defined how competency is monitored- method and frequency ( just included as part of the competency SOP) Orientation and initial training documentation in a checklist for general lab, safety Training checklist on each technical bench, instrument, major procedure PT records and performance/results > DO- a practical assessment ( block, slides, stains), for the assessment of previously analyzed specimens, and a PI feedback checklist for the technical from this audit of issues- how/what to improve Check off in performing QC, calibration, patient ID procedures ( acceptable error rates), examples for file DO of grossing, other performance such as instrument programming/maintenance Written quiz, policies & procedures, troubleshooting( problem solving documentation) Continuing education participation records > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: smah2@msn.com > > To: amber.mckenzie@gastrodocs.net; > > ronald.houston@nationwidechildrens.org; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 9 Apr 2014 11:10:18 +0000 > > Subject: RE: [Histonet] RE: competency form > > CC: > > > > We were recently dinged by CAP for our competency assessments in all areas. While that's no longer my immediate responsibility, I would love to be able to assist the lab manager with some information so we can tweek our assessments if you all wouldn't mind sharing with me as well. > > Thanks a bunch. > > Sheila Haas > > MicroPath Laboratories, Inc. > > Quality Assurance Coordinator > > > > > From: amber.mckenzie@gastrodocs.net > > > To: Ronald.Houston@nationwidechildrens.org; > > > histonet@lists.utsouthwestern.edu > > > Date: Tue, 8 Apr 2014 21:17:44 +0000 > > > CC: > > > Subject: [Histonet] RE: competency form > > > > > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > > > Thanks, > > > Amber > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > > Houston, Ronald > > > Sent: Tuesday, April 08, 2014 2:37 PM > > > To: Histonet > > > Subject: [Histonet] competency form > > > > > > Can someone please share the competency form(s) they are using to satisfy CAP? > > > > > > I am having problems convincing our QA/Compliance folks of the > > > differences between testing in AP compared to the other lab > > > disciplines, who do read our test results > > > > > > Thanks > > > > > > Ronnie Houston, MS HT(ASCP)QIHC > > > Anatomic Pathology Manager > > > ChildLab, a Division of Nationwide Children's Hospital > > > www.childlab.com > > > > > > 700 Children's Drive > > > Columbus, OH 43205 > > > (P) 614-722-5450 > > > (F) 614-722-2899 > > > ronald.houston@nationwidechildrens.org > > idechildrens.org> > > > www.NationwideChildrens.org > > > > > > "One person with passion is better than forty people merely interested." > > > ~ E.M. Forster > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tbraud <@t> holyredeemer.com Wed Apr 9 12:12:58 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Apr 9 12:13:04 2014 Subject: [Histonet] Re: High complexity testing In-Reply-To: <20140409162027.B92F41E8079@trendmess-svr.holyredeemer.local> References: <20140409162027.B92F41E8079@trendmess-svr.holyredeemer.local> Message-ID: Just to clarify, IHC is not high complexity testing. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 12 Date: Wed, 9 Apr 2014 12:26:47 +0000 From: joelle weaver Subject: RE: [Histonet] RE: competency form Is the question that Ronnie posed yesterday requesting justification of the need and extent of competency documentation for histology, or is it just a form needed? The general checklist pretty much sums up the necessity for doing, and required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding? As for a form, I would expect that the specific items on any forms will vary by your personnel and by the testing and processes you perform. What I did to document initial training and competency was a make a summary checklist for each "bench" with tasks and direct observations "DO" for initial training & documentation of satisfactory performance before patient testing. I just put all those checklists together in a summary table for each person. High complexity; such as grossing, IHC, FISH scoring get more attention and documentation, the waived tests, you have more discretion,- but I thought it easier to do everything about the same. I have not been inspected on this document yet ( so can't say if CAP will have issues with it- but will know soon...) but here is basically what I did to meet GEN.55500 or the main parts; Defined how competency is monitored- method and frequency ( just included as part of the competency SOP) Orientation and initial training documentation in a checklist for general lab, safety Training checklist on each technical bench, instrument, major procedure PT records and performance/results DO- a practical assessment ( block, slides, stains), for the assessment of previously analyzed specimens, and a PI feedback checklist for the technical from this audit of issues- how/what to improve Check off in performing QC, calibration, patient ID procedures ( acceptable error rates), examples for file DO of grossing, other performance such as instrument programming/maintenance Written quiz, policies & procedures, troubleshooting( problem solving documentation) Continuing education participation records Joelle Weaver MAOM, HTL (ASCP) QIHC --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From tbraud <@t> holyredeemer.com Wed Apr 9 12:45:08 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Apr 9 12:45:10 2014 Subject: [Histonet] RE: Competency checklist In-Reply-To: <20140409162027.B92F41E8079@trendmess-svr.holyredeemer.local> References: <20140409162027.B92F41E8079@trendmess-svr.holyredeemer.local> Message-ID: I have an extensive competency form that has passed many inspections. I will be glad to email it to anyone who is interested. I, too, struggled to make QA/QC compliance folks understand that this is not testing. I tell them that what we do is prep work and that the interpretation of the slides is the "test". I finally gave up. They don't care, they just want a competency checklist, so they got one, lol. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. competency form (Houston, Ronald) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From llang <@t> aipathology.com Wed Apr 9 14:07:13 2014 From: llang <@t> aipathology.com (LeAnn Lang) Date: Wed Apr 9 14:07:18 2014 Subject: [Histonet] Telepathology Message-ID: This question may have already been asked, so sorry for redundancy. Are any of your labs using telepathology for frozen sections? If so, what company are you working with? <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com From Hlee.Vue <@t> beaumont.edu Wed Apr 9 14:10:45 2014 From: Hlee.Vue <@t> beaumont.edu (Hlee Vue) Date: Wed Apr 9 14:10:49 2014 Subject: [Histonet] PLA2R1 Antibody Protocol Message-ID: Hi Histonet, I am working up the PLA2R1 antibody on a ventana benchmark optiview, and am having false positive staining with the negative control I am using. Is anyone working up the PLA2R1 antibody, if so what is your protocol for this antibody? What fixative are you using for your kidney biopsies for using this antibody? signed stressed student From smah2 <@t> msn.com Wed Apr 9 14:22:41 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Wed Apr 9 14:22:48 2014 Subject: [Histonet] Telepathology In-Reply-To: References: Message-ID: We are looking into Sakura's slide scanner for frozen sections. We looked at several a few months back and liked the Sakura model best but put it on the backburner. Now we're ready to purchase. Hope this helps. Sheila Haas MicroPath Laboratories, Inc. Lakeland, FL > From: llang@aipathology.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 9 Apr 2014 19:07:13 +0000 > Subject: [Histonet] Telepathology > > This question may have already been asked, so sorry for redundancy. > > Are any of your labs using telepathology for frozen sections? If so, what company are you working with? > > <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> > LeAnn Lang > Associates in Pathology > Practice Administrator > Phone: 715-847-0075 (ext 50259) > llang@aipathology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Wed Apr 9 14:27:17 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Wed Apr 9 14:25:57 2014 Subject: [Histonet] Telepathology In-Reply-To: References: Message-ID: <00d101cf5429$b86828e0$29387aa0$@caplab.org> We have used a WebEx session before. The remote site projects the microscopic image onto their desktop then shares the desktop. We also have one account that has an IP addressable microscope. This costs more but is more efficient. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang Sent: Wednesday, April 09, 2014 3:07 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Telepathology This question may have already been asked, so sorry for redundancy. Are any of your labs using telepathology for frozen sections? If so, what company are you working with? <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7317 - Release Date: 04/08/14 From joelleweaver <@t> hotmail.com Wed Apr 9 14:52:43 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Apr 9 14:52:47 2014 Subject: [Histonet] Re: High complexity testing In-Reply-To: References: <20140409162027.B92F41E8079@trendmess-svr.holyredeemer.local>, Message-ID: yes, I know- just meant the things that were more complicated- as in my checklist is longer. I mis-spoke, or "mis-typed" I guess. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Wed, 9 Apr 2014 13:12:58 -0400 > From: tbraud@holyredeemer.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: High complexity testing > > Just to clarify, IHC is not high complexity testing. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > > Message: 12 > Date: Wed, 9 Apr 2014 12:26:47 +0000 > From: joelle weaver > Subject: RE: [Histonet] RE: competency form > > Is the question that Ronnie posed yesterday requesting justification of > the need and extent of competency documentation for histology, or is it > just a form needed? The general checklist pretty much sums up the > necessity for doing, and required elements to me- GEN. 55500 and CLIA. > Maybe I am not understanding? > As for a form, I would expect that the specific items on any forms will > vary by your personnel and by the testing and processes you perform. > What I did to document initial training and competency was a make a > summary checklist for each "bench" with tasks and direct observations > "DO" for initial training & documentation of satisfactory performance > before patient testing. > I just put all those checklists together in a summary table for each > person. High complexity; such as grossing, IHC, FISH scoring get more > attention and documentation, the waived tests, you have more > discretion,- but I thought it easier to do everything about the same. I > have not been inspected on this document yet ( so can't say if CAP will > have issues with it- but will know soon...) but here is basically what I > did to meet GEN.55500 or the main parts; > > > Defined how competency is monitored- method and frequency ( just > included as part of the competency SOP) > Orientation and initial training documentation in a checklist for > general lab, safety > Training checklist on each technical bench, instrument, major procedure > PT records and performance/results > DO- a practical assessment ( block, slides, stains), for the assessment > of previously analyzed specimens, and a PI feedback checklist for the > technical from this audit of issues- how/what to improve > Check off in performing QC, calibration, patient ID procedures ( > acceptable error rates), examples for file > DO of grossing, other performance such as instrument > programming/maintenance > Written quiz, policies & procedures, troubleshooting( problem solving > documentation) > Continuing education participation records > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > --------------------------------------------------------------------------------- > > > > CONFIDENTIALITY NOTICE: > > This E-Mail is intended only for the use of the individual or entity to which > it was sent. It may contain information that is privileged and/or confidential, > and the use or disclosure of such information may also be restricted under applicable > federal and state law. If you received this communication in error, please do not > distribute any part of it or retain any copies, and delete the original E-Mail. > Please notify the sender of any error by E-Mail. > > Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llang <@t> aipathology.com Wed Apr 9 15:22:11 2014 From: llang <@t> aipathology.com (LeAnn Lang) Date: Wed Apr 9 15:22:17 2014 Subject: [Histonet] Telepathology In-Reply-To: <00d101cf5429$b86828e0$29387aa0$@caplab.org> References: <00d101cf5429$b86828e0$29387aa0$@caplab.org> Message-ID: Here is the scenario......we are located in one state and the hospital we want to provide frozen section coverage for is in another state. Can they have a histotech on site that can prepare the frozen and then send the image to my pathologist to read it? Does that person onsite have to be a pathologist assistant, or can the Pathologist use technology to guide the histotech on what sections to take? We are getting mixed answers on this. Some say yes, we can and others are saying because it is not FDA approved, we cannot use this as primary screening. Appreciate any input..... Like the idea of the WebEx too, thanks Douglas. And thank you Sheila for the info on Sakura. Has anyone used Remove Meeting Technologists (RMT) Software? Thank you, LeAnn -----Original Message----- From: Douglas Porter [mailto:doug.porter@caplab.org] Sent: Wednesday, April 09, 2014 2:27 PM To: LeAnn Lang; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Telepathology We have used a WebEx session before. The remote site projects the microscopic image onto their desktop then shares the desktop. We also have one account that has an IP addressable microscope. This costs more but is more efficient. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang Sent: Wednesday, April 09, 2014 3:07 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Telepathology This question may have already been asked, so sorry for redundancy. Are any of your labs using telepathology for frozen sections? If so, what company are you working with? <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7317 - Release Date: 04/08/14 From suetp918 <@t> comcast.net Wed Apr 9 15:37:55 2014 From: suetp918 <@t> comcast.net (Sue) Date: Wed Apr 9 15:38:04 2014 Subject: [Histonet] RE: competency form In-Reply-To: Message-ID: <816711670.1184553.1397075875058.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> So I went to one of the CAP webinars regarding competency? And when I asked what about histology there were complete silence on the other end of the line and than they said there was nothing for histology.? At our site they want something for very division so I put together a basic form and use it for the multitude of major tasks in histology.? We are not getting inspeted until next year but from what I hear CAP is planning on making major changes for the pathology protion of CAP's checklist, what that means I do not know.? Since CAP canot give us concrete rules for histology I think that anything a lab does is better than nothing, and shows initiative on their part to meet CAP's requirements. Since this is a peer review the inspector can suggest alternatives but cannot say you are in violation because the do not "like" your form. Just my thoughts S. Paturzo TJUH From vtobias <@t> uw.edu Wed Apr 9 15:36:17 2014 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Wed Apr 9 15:38:06 2014 Subject: [Histonet] Telepathology In-Reply-To: References: <00d101cf5429$b86828e0$29387aa0$@caplab.org> Message-ID: We just installed the VisionTek from Sakura. It is my understanding that the primary diagnosis cannot be made by telepathology at this time. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang Sent: Wednesday, April 09, 2014 1:22 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Telepathology Here is the scenario......we are located in one state and the hospital we want to provide frozen section coverage for is in another state. Can they have a histotech on site that can prepare the frozen and then send the image to my pathologist to read it? Does that person onsite have to be a pathologist assistant, or can the Pathologist use technology to guide the histotech on what sections to take? We are getting mixed answers on this. Some say yes, we can and others are saying because it is not FDA approved, we cannot use this as primary screening. Appreciate any input..... Like the idea of the WebEx too, thanks Douglas. And thank you Sheila for the info on Sakura. Has anyone used Remove Meeting Technologists (RMT) Software? Thank you, LeAnn -----Original Message----- From: Douglas Porter [mailto:doug.porter@caplab.org] Sent: Wednesday, April 09, 2014 2:27 PM To: LeAnn Lang; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Telepathology We have used a WebEx session before. The remote site projects the microscopic image onto their desktop then shares the desktop. We also have one account that has an IP addressable microscope. This costs more but is more efficient. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang Sent: Wednesday, April 09, 2014 3:07 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Telepathology This question may have already been asked, so sorry for redundancy. Are any of your labs using telepathology for frozen sections? If so, what company are you working with? <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7317 - Release Date: 04/08/14 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Wed Apr 9 15:41:23 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Wed Apr 9 15:40:10 2014 Subject: [Histonet] Telepathology In-Reply-To: References: <00d101cf5429$b86828e0$29387aa0$@caplab.org> Message-ID: <00d601cf5434$12f125a0$38d370e0$@caplab.org> Regarding sectioning of the specimen for frozen section, we had a video camera set up so the pathologist could view the specimen and direct the "person" on the other end how and what to section. We ended up not using this scenario, but had planned to see if it was an "approved" method. I suspect you'd have to have a PA or equivalent person on the specimen end for it to be "approved". We used WebEx for this also. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: LeAnn Lang [mailto:llang@aipathology.com] Sent: Wednesday, April 09, 2014 4:22 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Telepathology Here is the scenario......we are located in one state and the hospital we want to provide frozen section coverage for is in another state. Can they have a histotech on site that can prepare the frozen and then send the image to my pathologist to read it? Does that person onsite have to be a pathologist assistant, or can the Pathologist use technology to guide the histotech on what sections to take? We are getting mixed answers on this. Some say yes, we can and others are saying because it is not FDA approved, we cannot use this as primary screening. Appreciate any input..... Like the idea of the WebEx too, thanks Douglas. And thank you Sheila for the info on Sakura. Has anyone used Remove Meeting Technologists (RMT) Software? Thank you, LeAnn -----Original Message----- From: Douglas Porter [mailto:doug.porter@caplab.org] Sent: Wednesday, April 09, 2014 2:27 PM To: LeAnn Lang; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Telepathology We have used a WebEx session before. The remote site projects the microscopic image onto their desktop then shares the desktop. We also have one account that has an IP addressable microscope. This costs more but is more efficient. Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org ? ? The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LeAnn Lang Sent: Wednesday, April 09, 2014 3:07 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Telepathology This question may have already been asked, so sorry for redundancy. Are any of your labs using telepathology for frozen sections? If so, what company are you working with? <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang@aipathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7317 - Release Date: 04/08/14 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7317 - Release Date: 04/08/14 From suetp918 <@t> comcast.net Wed Apr 9 15:41:46 2014 From: suetp918 <@t> comcast.net (Sue) Date: Wed Apr 9 15:42:02 2014 Subject: [Histonet] Telepathology In-Reply-To: Message-ID: <1226708669.1184653.1397076106539.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Dissection for frozen sections is a high complexity task, there may be an issue with the histologist.? I am not so sure about usnig telepathology for the pathologist to ID where to take the section.? This is touchy and I would hate to put the tech in the position if there was an error. S. Paturzo TJUH From smah2 <@t> msn.com Wed Apr 9 16:33:37 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Wed Apr 9 16:33:41 2014 Subject: [Histonet] RE: competency form In-Reply-To: <816711670.1184553.1397075875058.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> References: <816711670.1184553.1397075875058.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: Well, it would be nice if that was the case but we ended up with a deficiency. It was rather disappointing given our overall documentation. Sent from my iPhone > On Apr 9, 2014, at 4:37 PM, "Sue" wrote: > > So I went to one of the CAP webinars regarding competency And when I asked what about > > histology there were complete silence on the other end of the line and than they said there was > > nothing for histology. > > > > At our site they want something for very division so I put together a basic form and use it > > for the multitude of major tasks in histology. We are not getting inspeted until next year but > > from what I hear CAP is planning on making major changes for the pathology protion of > > CAP's checklist, what that means I do not know. > > > > Since CAP canot give us concrete rules for histology I think that anything a lab does is > > better than nothing, and shows initiative on their part to meet CAP's requirements. > > Since this is a peer review the inspector can suggest alternatives but > > cannot say you are in violation because the do not "like" your form. > > > > Just my thoughts > > > > S. Paturzo > > TJUH > > > > From Glenn.Hauck <@t> albertahealthservices.ca Wed Apr 9 16:44:14 2014 From: Glenn.Hauck <@t> albertahealthservices.ca (Glenn Hauck) Date: Wed Apr 9 16:44:20 2014 Subject: [Histonet] Gi-Bx's Message-ID: <38FA8FCA474F834CB9B90590D7C72390015B45CAB931@EXMBX4C.crha.bewell.ca> We have 4 slides with 2 section on each. We take a ribbon put 2 sections for unstained and 2 sections for H&E pare in about 200um depending on how much tissue there is then re-soak cool and take2 sections for unstained and 2 sections H&E. We do this for all colon Bx's. We re-soak to prevent the chatter you see with these types of Bx's. Hope this helps Glenn ________________________________ This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From joelleweaver <@t> hotmail.com Wed Apr 9 16:50:45 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Apr 9 16:50:47 2014 Subject: [Histonet] RE: competency form In-Reply-To: <816711670.1184553.1397075875058.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> References: , <816711670.1184553.1397075875058.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> Message-ID: I appreciate your thoughts. True, seems like they couldn't be too specific in their requirements if they cannot list specifically what they require ( when you specifically ask), what a sentence! We will all find out if that is the case or not soon enough. Anyhow, I just want to agree with you that all the signs seem to be that there will be tightening, based on their magazine articles and web site postings, etc; seems like validation & verification, competency, PT & ( interlaboratory correlation) will be at the top of the list...stay tuned. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 9 Apr 2014 20:37:55 +0000 From: suetp918@comcast.net To: joelleweaver@hotmail.com CC: trathborne@somerset-healthcare.com; smah2@msn.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: competency form So I went to one of the CAP webinars regarding competency And when I asked what about histology there were complete silence on the other end of the line and than they said there was nothing for histology. At our site they want something for very division so I put together a basic form and use it for the multitude of major tasks in histology. We are not getting inspeted until next year but from what I hear CAP is planning on making major changes for the pathology protion of CAP's checklist, what that means I do not know. Since CAP canot give us concrete rules for histology I think that anything a lab does is better than nothing, and shows initiative on their part to meet CAP's requirements. Since this is a peer review the inspector can suggest alternatives but cannot say you are in violation because the do not "like" your form. Just my thoughts S. Paturzo TJUH From amber.mckenzie <@t> gastrodocs.net Wed Apr 9 17:08:03 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Apr 9 17:08:08 2014 Subject: [Histonet] CPT codes In-Reply-To: References: <20140409162027.B92F41E8079@trendmess-svr.holyredeemer.local>, Message-ID: <5A33C952BB67F4468AF1F36D739212BC011247EA28@JERRY.Gia.com> Does anyone know where I can find a list of all the special stains/IHC stains with their appropriate CPT code? Is there a book to buy or can I google it and print something off? Thanks! From suetp918 <@t> comcast.net Wed Apr 9 17:25:50 2014 From: suetp918 <@t> comcast.net (Sue) Date: Wed Apr 9 17:26:05 2014 Subject: [Histonet] CPT codes In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011247EA28@JERRY.Gia.com> Message-ID: <1293924247.1186945.1397082350024.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> just get the coding book From ibernard <@t> uab.edu Wed Apr 9 20:04:38 2014 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Wed Apr 9 20:04:44 2014 Subject: [Histonet] RE: competency form In-Reply-To: References: , , , <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com>, , , , , , <3AD061FE740D464FAC7BF6B5CFB75707A95C7064@SMCMAIL01.somerset-healthcare.com> Message-ID: There is a great need for standardization in histotechnology, from forms, policies, procedures/methods to controls processes and protocols. Hope NSH is working with ASCP and CAP to make this happen. MSgt Ian R Bernard, HT(ASCP), MSHA-UAB Anatomic Pathology Lab Manager USAF- Active Duty 210-687-7540 Cell ibernard@uab.edu ian.bernard@comcast.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Wednesday, April 09, 2014 7:09 AM To: Rathborne, Toni; 'Sheila Haas'; Amber McKenzie; Houston, Ronald; Histonet Subject: RE: [Histonet] RE: competency form True there is inspector variation. Maybe we should all pool our resources and see what we can come up with collaboratively? It is great to know what the CAP feedback has been so we can all fill in any gaps. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: trathborne@somerset-healthcare.com > To: smah2@msn.com; joelleweaver@hotmail.com; > amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: competency form > Date: Wed, 9 Apr 2014 13:04:17 +0000 > > CAP inspectors may have opinions which differ from our own, and their interpretation of standards may also be different. Have you challenged this deficiency with CAP? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila > Haas > Sent: Wednesday, April 09, 2014 8:52 AM > To: joelle weaver; Amber McKenzie; Houston, Ronald; Histonet > Subject: RE: [Histonet] RE: competency form > > What we had here, which did not meet the CAP inspectors requirements apparently, were the procedure (of course); a form with each observation of each task documented along with any corrective action necessary; the correlation of proficiency tests, educational assessments and performance reviews for technical staff; daily evaluations from the pathologists concerning staining, microtomy and grossing; and educational training documentation. We had no idea with all pieces of this documentation that we were anticipated to have more. The form for DO of each task was not detailed enough (despite listing each task and proficiency or corrective action of each task) according to the inspectors. I was hoping someone could share a form so as to assist us in seeing what holes there are in our form. While this DO form is definitely not our only form, the inspectors specifically commented on this one. > > If anyone can assist, it would be helpful. > Thank you, > Sheila Haas > MicroPath Laboratories, Inc. > Quality Assurance Coordinator > > > From: joelleweaver@hotmail.com > To: smah2@msn.com; amber.mckenzie@gastrodocs.net; > ronald.houston@nationwidechildrens.org; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: competency form > Date: Wed, 9 Apr 2014 12:26:47 +0000 > > > > > > Is the question that Ronnie posed yesterday requesting justification of the need and extent of competency documentation for histology, or is it just a form needed? The general checklist pretty much sums up the necessity for doing, and required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding? > As for a form, I would expect that the specific items on any forms will vary by your personnel and by the testing and processes you perform. > What I did to document initial training and competency was a make a summary checklist for each "bench" with tasks and direct observations "DO" for initial training & documentation of satisfactory performance before patient testing. > I just put all those checklists together in a summary table for each > person. High complexity; such as grossing, IHC, FISH scoring get more > attention and documentation, the waived tests, you have more > discretion,- but I thought it easier to do everything about the same. > I have not been inspected on this document yet ( so can't say if CAP > will have issues with it- but will know soon...) but here is basically > what I did to meet GEN.55500 or the main parts; > > > Defined how competency is monitored- method and frequency ( just > included as part of the competency SOP) Orientation and initial > training documentation in a checklist for general lab, safety Training > checklist on each technical bench, instrument, major procedure PT > records and performance/results > DO- a practical assessment ( block, slides, stains), for the > assessment of previously analyzed specimens, and a PI feedback > checklist for the technical from this audit of issues- how/what to > improve Check off in performing QC, calibration, patient ID procedures > ( acceptable error rates), examples for file DO of grossing, other > performance such as instrument programming/maintenance Written quiz, > policies & procedures, troubleshooting( problem solving documentation) > Continuing education participation records > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: smah2@msn.com > > To: amber.mckenzie@gastrodocs.net; > > ronald.houston@nationwidechildrens.org; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 9 Apr 2014 11:10:18 +0000 > > Subject: RE: [Histonet] RE: competency form > > CC: > > > > We were recently dinged by CAP for our competency assessments in all areas. While that's no longer my immediate responsibility, I would love to be able to assist the lab manager with some information so we can tweek our assessments if you all wouldn't mind sharing with me as well. > > Thanks a bunch. > > Sheila Haas > > MicroPath Laboratories, Inc. > > Quality Assurance Coordinator > > > > > From: amber.mckenzie@gastrodocs.net > > > To: Ronald.Houston@nationwidechildrens.org; > > > histonet@lists.utsouthwestern.edu > > > Date: Tue, 8 Apr 2014 21:17:44 +0000 > > > CC: > > > Subject: [Histonet] RE: competency form > > > > > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > > > Thanks, > > > Amber > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > > Houston, Ronald > > > Sent: Tuesday, April 08, 2014 2:37 PM > > > To: Histonet > > > Subject: [Histonet] competency form > > > > > > Can someone please share the competency form(s) they are using to satisfy CAP? > > > > > > I am having problems convincing our QA/Compliance folks of the > > > differences between testing in AP compared to the other lab > > > disciplines, who do read our test results > > > > > > Thanks > > > > > > Ronnie Houston, MS HT(ASCP)QIHC > > > Anatomic Pathology Manager > > > ChildLab, a Division of Nationwide Children's Hospital > > > www.childlab.com > > > > > > 700 Children's Drive > > > Columbus, OH 43205 > > > (P) 614-722-5450 > > > (F) 614-722-2899 > > > ronald.houston@nationwidechildrens.org > > nw > > > idechildrens.org> > > > www.NationwideChildrens.org > > > > > > "One person with passion is better than forty people merely interested." > > > ~ E.M. Forster > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ibernard <@t> uab.edu Wed Apr 9 20:57:10 2014 From: ibernard <@t> uab.edu (Ian R Bernard) Date: Wed Apr 9 20:57:20 2014 Subject: [Histonet] RE: competency form- Inspector Perspective. Message-ID: I recently had the great honor to serve as a CAP inspector in Arizona. The lab we inspected will be accredited. Lest we forget as inspectors or future inspectors, I hope the below perspective illuminates the purpose of the CAP inspection and helps preserve the integrity of the program if adhered to. As inspectors, we are trained to be thorough but to simultaneously use "objective judgment" i.e. " there may be several ways to meet the intent of the question" and that "we must review all pertinent documentation and then determine if the process meets the intent of the requirement". Keeping in mind that the purpose of CAP inspection is not punitive but "quality laboratory improvement to fulfill the regulatory purpose of the inspection (CLIA)". Our focus should be, in the interest of time and thoroughness "major compliance issue rather than nitpick". This helps us maintain "professionalism and preserve the peer-review nature of the program". So what is it that constitutes compliance? Per CAP: "- One, that the laboratory has defined a policy, a procedure, or a plan "of the three P's" for how they are going to do things in the lab. - Secondly, actual practices that matches those three P's. - Finally, documentation to support the fact that practice has indeed matched policies and procedures." So what is a deficiency? Per CAP: "A deficiency means that the lab did not meet the intent of the checklist item. It's not the wording; it's not the specifics. It is the intent". If any of the three above criteria are not yet met, we should cite a deficiency" We are admonished to remember that "there may be many ways to accomplish an objective. The lab may not do things exactly the way that your lab does, but may still be meeting the intent of the requirement(s). Citing a laboratory for "not doing it the way we do it" is a common inspector error." Per CAP, partial compliance is the following: "If there is partial compliance (e.g., some records are inconsistent, one bottle of reagent was not labeled completely, a few temperatures were not recorded, etc.), you must judge whether the degree of non-compliance is likely to have adverse effects on test accuracy, patient care, or worker safety. Also, determine if the lab staff was aware of the inconsistency and if corrective actions were performed. If adverse effects are likely or if there are definite patterns (e.g., missing temperatures only on weekends) without corrective actions" only then we must cite a deficiency. Bottom-line, If you feel you were incorrectly cited since you met the intent of the question, you should appeal to CAP. It is an inspected lab's right. If a phase 11 deficiency, submit your evidence of compliance and the Lab Accreditation Committee will either overrule or sustain. I suspect they will overrule. Just saying, but based upon just what you described, it sounds like you all met the intent of the question. MSgt Ian R Bernard, HT(ASCP), MSHA-UAB Anatomic Pathology Lab Manager USAF- Active Duty ibernard@uab.edu ian.bernard@comcast.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, April 09, 2014 7:04 AM To: 'Sheila Haas'; joelle weaver; Amber McKenzie; Houston, Ronald; Histonet Subject: RE: [Histonet] RE: competency form CAP inspectors may have opinions which differ from our own, and their interpretation of standards may also be different. Have you challenged this deficiency with CAP? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Wednesday, April 09, 2014 8:52 AM To: joelle weaver; Amber McKenzie; Houston, Ronald; Histonet Subject: RE: [Histonet] RE: competency form What we had here, which did not meet the CAP inspectors requirements apparently, were the procedure (of course); a form with each observation of each task documented along with any corrective action necessary; the correlation of proficiency tests, educational assessments and performance reviews for technical staff; daily evaluations from the pathologists concerning staining, microtomy and grossing; and educational training documentation. We had no idea with all pieces of this documentation that we were anticipated to have more. The form for DO of each task was not detailed enough (despite listing each task and proficiency or corrective action of each task) according to the inspectors. I was hoping someone could share a form so as to assist us in seeing what holes there are in our form. While this DO form is definitely not our only form, the inspectors specifically commented on this one. If anyone can assist, it would be helpful. Thank you, Sheila Haas MicroPath Laboratories, Inc. Quality Assurance Coordinator From: joelleweaver@hotmail.com To: smah2@msn.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: competency form Date: Wed, 9 Apr 2014 12:26:47 +0000 Is the question that Ronnie posed yesterday requesting justification of the need and extent of competency documentation for histology, or is it just a form needed? The general checklist pretty much sums up the necessity for doing, and required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding? As for a form, I would expect that the specific items on any forms will vary by your personnel and by the testing and processes you perform. What I did to document initial training and competency was a make a summary checklist for each "bench" with tasks and direct observations "DO" for initial training & documentation of satisfactory performance before patient testing. I just put all those checklists together in a summary table for each person. High complexity; such as grossing, IHC, FISH scoring get more attention and documentation, the waived tests, you have more discretion,- but I thought it easier to do everything about the same. I have not been inspected on this document yet ( so can't say if CAP will have issues with it- but will know soon...) but here is basically what I did to meet GEN.55500 or the main parts; Defined how competency is monitored- method and frequency ( just included as part of the competency SOP) Orientation and initial training documentation in a checklist for general lab, safety Training checklist on each technical bench, instrument, major procedure PT records and performance/results DO- a practical assessment ( block, slides, stains), for the assessment of previously analyzed specimens, and a PI feedback checklist for the technical from this audit of issues- how/what to improve Check off in performing QC, calibration, patient ID procedures ( acceptable error rates), examples for file DO of grossing, other performance such as instrument programming/maintenance Written quiz, policies & procedures, troubleshooting( problem solving documentation) Continuing education participation records Joelle Weaver MAOM, HTL (ASCP) QIHC > From: smah2@msn.com > To: amber.mckenzie@gastrodocs.net; > ronald.houston@nationwidechildrens.org; > histonet@lists.utsouthwestern.edu > Date: Wed, 9 Apr 2014 11:10:18 +0000 > Subject: RE: [Histonet] RE: competency form > CC: > > We were recently dinged by CAP for our competency assessments in all areas. While that's no longer my immediate responsibility, I would love to be able to assist the lab manager with some information so we can tweek our assessments if you all wouldn't mind sharing with me as well. > Thanks a bunch. > Sheila Haas > MicroPath Laboratories, Inc. > Quality Assurance Coordinator > > > From: amber.mckenzie@gastrodocs.net > > To: Ronald.Houston@nationwidechildrens.org; > > histonet@lists.utsouthwestern.edu > > Date: Tue, 8 Apr 2014 21:17:44 +0000 > > CC: > > Subject: [Histonet] RE: competency form > > > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > > Thanks, > > Amber > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > Houston, Ronald > > Sent: Tuesday, April 08, 2014 2:37 PM > > To: Histonet > > Subject: [Histonet] competency form > > > > Can someone please share the competency form(s) they are using to satisfy CAP? > > > > I am having problems convincing our QA/Compliance folks of the > > differences between testing in AP compared to the other lab > > disciplines, who do read our test results > > > > Thanks > > > > Ronnie Houston, MS HT(ASCP)QIHC > > Anatomic Pathology Manager > > ChildLab, a Division of Nationwide Children's Hospital > > www.childlab.com > > > > 700 Children's Drive > > Columbus, OH 43205 > > (P) 614-722-5450 > > (F) 614-722-2899 > > ronald.houston@nationwidechildrens.org > idechildrens.org> > > www.NationwideChildrens.org > > > > "One person with passion is better than forty people merely interested." > > ~ E.M. Forster > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdemarinis <@t> SARATOGACARE.ORG Thu Apr 10 05:53:30 2014 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Thu Apr 10 05:53:42 2014 Subject: [Histonet] RE: competency form In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com> References: <5A33C952BB67F4468AF1F36D739212BC011247DF3D@JERRY.Gia.com> Message-ID: <1C75A843982A7B44BB368A3CC946ABCA0A70E292@SHEXCHMBX01.SARAHOSP.ORG> I would be interested in reviewing the competency form. Could you email it to me as well? Thanks. Carolyn - cdemarinis@saratogacare.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, April 08, 2014 5:18 PM To: Houston, Ronald; Histonet Subject: [Histonet] RE: competency form Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. Thanks, Amber -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, April 08, 2014 2:37 PM To: Histonet Subject: [Histonet] competency form Can someone please share the competency form(s) they are using to satisfy CAP? I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From smah2 <@t> msn.com Thu Apr 10 07:02:41 2014 From: smah2 <@t> msn.com (Sheila Haas) Date: Thu Apr 10 07:02:46 2014 Subject: [Histonet] RE: competency form- Inspector Perspective. In-Reply-To: References: Message-ID: Well said Ian and I couldn't agree more. I certainly understand the need for consistency and regulations. We are appealing a couple of our deficiencies because we have the documentation although it was not documented the way the inspectors wanted to see it (as part of a particular form); instead we have a couple of forms which captures the information in a number of ways. We'll see what CAP says...stay tuned. Thanks to everyone for their input and information. It is greatly appreciated. Sheila Haas MicroPath Laboratories, Inc. > From: ibernard@uab.edu > To: trathborne@somerset-healthcare.com; smah2@msn.com; joelleweaver@hotmail.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: competency form- Inspector Perspective. > Date: Thu, 10 Apr 2014 01:57:10 +0000 > > I recently had the great honor to serve as a CAP inspector in Arizona. The lab we inspected will be accredited. > > Lest we forget as inspectors or future inspectors, I hope the below perspective illuminates the purpose of the CAP inspection and helps preserve the integrity of the program if adhered to. > > As inspectors, we are trained to be thorough but to simultaneously use "objective judgment" i.e. " there may be several ways to meet the intent of the question" and that "we must review all pertinent documentation and then determine if the process meets the intent of the requirement". > > Keeping in mind that the purpose of CAP inspection is not punitive but "quality laboratory improvement to fulfill the regulatory purpose of the inspection (CLIA)". Our focus should be, in the interest of time and thoroughness "major compliance issue rather than nitpick". This helps us maintain "professionalism and preserve the peer-review nature of the program". > > So what is it that constitutes compliance? > Per CAP: > "- One, that the laboratory has defined a policy, a procedure, or a plan "of the three P's" for how they are going to do things in the lab. > - Secondly, actual practices that matches those three P's. > - Finally, documentation to support the fact that practice has indeed matched policies and procedures." > > So what is a deficiency? > > Per CAP: > "A deficiency means that the lab did not meet the intent of the checklist item. It's not the wording; it's not the specifics. It is the intent". If any of the three above criteria are not yet met, we should cite a deficiency" We are admonished to remember that "there may be many ways to accomplish an objective. The lab may not do things exactly the way that your lab does, but may still be meeting the intent of the requirement(s). Citing a laboratory for "not doing it the way we do it" is a common inspector error." > > Per CAP, partial compliance is the following: "If there is partial compliance (e.g., some records are inconsistent, one bottle of reagent was not labeled completely, a few temperatures were not recorded, etc.), you must judge whether the degree of non-compliance is likely to have adverse effects on test accuracy, patient care, or worker safety. Also, determine if the lab staff was aware of the inconsistency and if corrective actions were performed. If adverse effects are likely or if there are definite patterns (e.g., missing temperatures only on weekends) without corrective actions" only then we must cite a deficiency. > > Bottom-line, If you feel you were incorrectly cited since you met the intent of the question, you should appeal to CAP. It is an inspected lab's right. If a phase 11 deficiency, submit your evidence of compliance and the Lab Accreditation Committee will either overrule or sustain. I suspect they will overrule. > > Just saying, but based upon just what you described, it sounds like you all met the intent of the question. > > MSgt Ian R Bernard, HT(ASCP), MSHA-UAB > Anatomic Pathology Lab Manager > USAF- Active Duty > ibernard@uab.edu > ian.bernard@comcast.net > > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni > Sent: Wednesday, April 09, 2014 7:04 AM > To: 'Sheila Haas'; joelle weaver; Amber McKenzie; Houston, Ronald; Histonet > Subject: RE: [Histonet] RE: competency form > > CAP inspectors may have opinions which differ from our own, and their interpretation of standards may also be different. Have you challenged this deficiency with CAP? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Wednesday, April 09, 2014 8:52 AM > To: joelle weaver; Amber McKenzie; Houston, Ronald; Histonet > Subject: RE: [Histonet] RE: competency form > > What we had here, which did not meet the CAP inspectors requirements apparently, were the procedure (of course); a form with each observation of each task documented along with any corrective action necessary; the correlation of proficiency tests, educational assessments and performance reviews for technical staff; daily evaluations from the pathologists concerning staining, microtomy and grossing; and educational training documentation. We had no idea with all pieces of this documentation that we were anticipated to have more. The form for DO of each task was not detailed enough (despite listing each task and proficiency or corrective action of each task) according to the inspectors. I was hoping someone could share a form so as to assist us in seeing what holes there are in our form. While this DO form is definitely not our only form, the inspectors specifically commented on this one. > > If anyone can assist, it would be helpful. > Thank you, > Sheila Haas > MicroPath Laboratories, Inc. > Quality Assurance Coordinator > > > From: joelleweaver@hotmail.com > To: smah2@msn.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: competency form > Date: Wed, 9 Apr 2014 12:26:47 +0000 > > > > > > Is the question that Ronnie posed yesterday requesting justification of the need and extent of competency documentation for histology, or is it just a form needed? The general checklist pretty much sums up the necessity for doing, and required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding? > As for a form, I would expect that the specific items on any forms will vary by your personnel and by the testing and processes you perform. > What I did to document initial training and competency was a make a summary checklist for each "bench" with tasks and direct observations "DO" for initial training & documentation of satisfactory performance before patient testing. > I just put all those checklists together in a summary table for each person. High complexity; such as grossing, IHC, FISH scoring get more attention and documentation, the waived tests, you have more discretion,- but I thought it easier to do everything about the same. I have not been inspected on this document yet ( so can't say if CAP will have issues with it- but will know soon...) but here is basically what I did to meet GEN.55500 or the main parts; > > > Defined how competency is monitored- method and frequency ( just included as part of the competency SOP) Orientation and initial training documentation in a checklist for general lab, safety Training checklist on each technical bench, instrument, major procedure PT records and performance/results > DO- a practical assessment ( block, slides, stains), for the assessment of previously analyzed specimens, and a PI feedback checklist for the technical from this audit of issues- how/what to improve Check off in performing QC, calibration, patient ID procedures ( acceptable error rates), examples for file DO of grossing, other performance such as instrument programming/maintenance Written quiz, policies & procedures, troubleshooting( problem solving documentation) Continuing education participation records > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: smah2@msn.com > > To: amber.mckenzie@gastrodocs.net; > > ronald.houston@nationwidechildrens.org; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 9 Apr 2014 11:10:18 +0000 > > Subject: RE: [Histonet] RE: competency form > > CC: > > > > We were recently dinged by CAP for our competency assessments in all areas. While that's no longer my immediate responsibility, I would love to be able to assist the lab manager with some information so we can tweek our assessments if you all wouldn't mind sharing with me as well. > > Thanks a bunch. > > Sheila Haas > > MicroPath Laboratories, Inc. > > Quality Assurance Coordinator > > > > > From: amber.mckenzie@gastrodocs.net > > > To: Ronald.Houston@nationwidechildrens.org; > > > histonet@lists.utsouthwestern.edu > > > Date: Tue, 8 Apr 2014 21:17:44 +0000 > > > CC: > > > Subject: [Histonet] RE: competency form > > > > > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > > > Thanks, > > > Amber > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > > Houston, Ronald > > > Sent: Tuesday, April 08, 2014 2:37 PM > > > To: Histonet > > > Subject: [Histonet] competency form > > > > > > Can someone please share the competency form(s) they are using to satisfy CAP? > > > > > > I am having problems convincing our QA/Compliance folks of the > > > differences between testing in AP compared to the other lab > > > disciplines, who do read our test results > > > > > > Thanks > > > > > > Ronnie Houston, MS HT(ASCP)QIHC > > > Anatomic Pathology Manager > > > ChildLab, a Division of Nationwide Children's Hospital > > > www.childlab.com > > > > > > 700 Children's Drive > > > Columbus, OH 43205 > > > (P) 614-722-5450 > > > (F) 614-722-2899 > > > ronald.houston@nationwidechildrens.org > > idechildrens.org> > > > www.NationwideChildrens.org > > > > > > "One person with passion is better than forty people merely interested." > > > ~ E.M. Forster > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Thu Apr 10 07:14:23 2014 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Apr 10 07:14:27 2014 Subject: [Histonet] Telepathology Message-ID: <8D122CD8B1265F0-688-483FC@webmail-vm018.sysops.aol.com> Victor Tobias asks about telepathology- I seem to remember there is a rule somewhere that primary diagnosis can only be made using a glass slide. As to chapter and verse, I don't know. However there are reports in the literarature that it has been tried out for F/S in Alaska and Finland. Michael Titford USA Pathology Mobile AL USA From cbrya <@t> lexclin.com Thu Apr 10 08:58:07 2014 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Thu Apr 10 08:58:13 2014 Subject: [Histonet] PA Schedule Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF4C035F5A92@EXCHANGESB> Hello, If your PAs cover multiple sites, do they manage their own schedules or do the pathologists direct them where to go and when? For instance, if the workflow is heavier at one location than the other, would they have the ability to use their own discretion to leave and go where needed most? Any input is greatly appreciated. Have a great day! Carol NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From cytch7 <@t> cox.net Thu Apr 10 09:36:01 2014 From: cytch7 <@t> cox.net (cytch7@cox.net) Date: Thu Apr 10 09:36:05 2014 Subject: [Histonet] RE: competency form In-Reply-To: Message-ID: <20140410103601.KLHN6.214661.imail@fed1rmwml208> I was having the same problems with developing competency records for my cytotechnologist. I found this article from ADVACE for Adminstrators to be very helpful in creating my competency forms. We just had an on-site CAP inspection and it seemed to satisfy them. Hope this link is helpful. The article's author is Teresa Scott. http://laboratory-manager.advanceweb.com/Web-Extras/Online-Extras/Competency-Assessment.aspx Valerie Biendara SCT(ASCP)IAC Cytology Supervisor NWA Pathology Associates ---- "Demarinis wrote: ============= I would be interested in reviewing the competency form. Could you email it to me as well? Thanks. Carolyn - cdemarinis@saratogacare.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, April 08, 2014 5:18 PM To: Houston, Ronald; Histonet Subject: [Histonet] RE: competency form Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. Thanks, Amber -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, April 08, 2014 2:37 PM To: Histonet Subject: [Histonet] competency form Can someone please share the competency form(s) they are using to satisfy CAP? I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Thu Apr 10 10:55:39 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu Apr 10 10:55:43 2014 Subject: [Histonet] Telepathology In-Reply-To: <8D122CD8B1265F0-688-483FC@webmail-vm018.sysops.aol.com> References: <8D122CD8B1265F0-688-483FC@webmail-vm018.sysops.aol.com> Message-ID: In the US, FDA has designated the WSI instruments as Class I and, to date, no company has received approval/clearance for use. Until the FDA issue is resolved, the use of WSI for primary diagnosis is prohibited. Outside the US, WSI can be used in all facets of pathology, including primary diagnosis, and is used extensively. That said, in the US, using WSI for pathology processes such as frozen section diagnosis, case consultation, special stain and IHC review, can occur because these processes do not produce a primary diagnosis. There are many other uses for WSI in pathology lab that make the consideration for use and implementation cost effective. CAP has released guidelines for validating WSI in the lab and there is the option to expand the use of WSI by using the "laboratory developed test" route. The best resource for WSI, in my mind, is the Digital Pathology Association (DPA) web site. I am a member and this group is dedicated to the advancement and education of WSI. Check out the web site William DeSalvo, BS HTL(ASCP) > To: histonet@lists.utsouthwestern.edu > From: mtitford@aol.com > Date: Thu, 10 Apr 2014 08:14:23 -0400 > Subject: [Histonet] Telepathology > > > Victor Tobias asks about telepathology- > > I seem to remember there is a rule somewhere that primary diagnosis can only be made using a glass slide. As to chapter and verse, I don't know. > > However there are reports in the literarature that it has been tried out for F/S in Alaska and Finland. > > Michael Titford > USA Pathology > Mobile AL USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Apr 10 11:41:30 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 10 11:41:39 2014 Subject: [Histonet] Telepathology In-Reply-To: References: <8D122CD8B1265F0-688-483FC@webmail-vm018.sysops.aol.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05862@SBS2K8.premierlab.local> Here is my two cents and a bit of a rant, Bill you got me going. I agree with Bill - just want to add my thoughts - it's a Class III Medical Device (meaning that they have classified this type of equipment as highly-regulated and high-risk) requiring a pre-market approval process. I personally think that prohibited is too strong of a word. Like Bill says the CAP has guidelines on how to utilized this technology for other processes such as primary diagnosis through the "laboratory developed test" route. I'm not sure what that FDA would do if they found out a lab was utilizing this technology for primary diagnosis or how that would affect billing capabilities, there is probably some risk associated with this approach. If you look at some of the FDA published guidelines many of them recommend the use of digital pathology. There are many instruments currently on the market that have various 510K clearances for algorithms, digital reads, etc. The DPA website has all of this information on it and much more regarding WSI - www.digitalpathologyassociation.org. My impression of what CAP is saying - they are saying move forward with this technology and they have provided us with guidelines on how to do so. Bottom line this is technology that can improve patient care and we in the US are so far behind in implementing this technology to its fullest potential, like Bill says outside the US its used extensively for primary diagnosis. If you are afraid of the risk of primary diagnosis, use this technology for one of the many processes that Bill has listed below. It also can be a valuable resource to the histology lab in general, you can use it in QC, tracking control staining, in IHC protocol development, consistency of staining, etc. It's a great tool. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, April 10, 2014 9:56 AM To: mtitford@aol.com; histonet Subject: RE: [Histonet] Telepathology In the US, FDA has designated the WSI instruments as Class I and, to date, no company has received approval/clearance for use. Until the FDA issue is resolved, the use of WSI for primary diagnosis is prohibited. Outside the US, WSI can be used in all facets of pathology, including primary diagnosis, and is used extensively. That said, in the US, using WSI for pathology processes such as frozen section diagnosis, case consultation, special stain and IHC review, can occur because these processes do not produce a primary diagnosis. There are many other uses for WSI in pathology lab that make the consideration for use and implementation cost effective. CAP has released guidelines for validating WSI in the lab and there is the option to expand the use of WSI by using the "laboratory developed test" route. The best resource for WSI, in my mind, is the Digital Pathology Association (DPA) web site. I am a member and this group is dedicated to the advancement and education of WSI. Check out the web site William DeSalvo, BS HTL(ASCP) > To: histonet@lists.utsouthwestern.edu > From: mtitford@aol.com > Date: Thu, 10 Apr 2014 08:14:23 -0400 > Subject: [Histonet] Telepathology > > > Victor Tobias asks about telepathology- > > I seem to remember there is a rule somewhere that primary diagnosis can only be made using a glass slide. As to chapter and verse, I don't know. > > However there are reports in the literarature that it has been tried out for F/S in Alaska and Finland. > > Michael Titford > USA Pathology > Mobile AL USA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Apr 10 12:29:48 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 10 12:29:53 2014 Subject: [Histonet] Award Spotlight - Leica Leadership in Teaching Award Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05865@SBS2K8.premierlab.local> Hello Histonetters Here is some information on one of the many awards that NSH has to offer: Award Spotlight - Leica Leadership In Teaching Award Leica Biosystems sponsors this $1000.00 award. This award is given annually to an individual dedicated to teaching Histotechnology exemplifying the qualities of a dedicated teacher sharing their knowledge with others and advancing the growth of the profession of Histotechnology. Last year's recipient was Elaine Basham. Do you know of a person who is a dedicated teacher of Histotechnology then nominate them for this award http://www.nsh.org/scholarships-awards/leadership/individual%20awards Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From joelleweaver <@t> hotmail.com Thu Apr 10 12:30:13 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Apr 10 12:30:17 2014 Subject: [Histonet] Telepathology In-Reply-To: References: <8D122CD8B1265F0-688-483FC@webmail-vm018.sysops.aol.com>, Message-ID: There is a nice publication on validation of WSI on their site, similar to LDT. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: wdesalvo.cac@outlook.com > To: mtitford@aol.com; histonet@lists.utsouthwestern.edu > Date: Thu, 10 Apr 2014 08:55:39 -0700 > Subject: RE: [Histonet] Telepathology > CC: > > In the US, FDA has designated the WSI instruments as Class I and, to date, no company has received approval/clearance for use. Until the FDA issue is resolved, the use of WSI for primary diagnosis is prohibited. Outside the US, WSI can be used in all facets of pathology, including primary diagnosis, and is used extensively. > > That said, in the US, using WSI for pathology processes such as frozen section diagnosis, case consultation, special stain and IHC review, can occur because these processes do not produce a primary diagnosis. There are many other uses for WSI in pathology lab that make the consideration for use and implementation cost effective. > > CAP has released guidelines for validating WSI in the lab and there is the option to expand the use of WSI by using the "laboratory developed test" route. > > The best resource for WSI, in my mind, is the Digital Pathology Association (DPA) web site. I am a member and this group is dedicated to the advancement and education of WSI. Check out the web site > > > William DeSalvo, BS HTL(ASCP) > > > To: histonet@lists.utsouthwestern.edu > > From: mtitford@aol.com > > Date: Thu, 10 Apr 2014 08:14:23 -0400 > > Subject: [Histonet] Telepathology > > > > > > Victor Tobias asks about telepathology- > > > > I seem to remember there is a rule somewhere that primary diagnosis can only be made using a glass slide. As to chapter and verse, I don't know. > > > > However there are reports in the literarature that it has been tried out for F/S in Alaska and Finland. > > > > Michael Titford > > USA Pathology > > Mobile AL USA > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Apr 10 12:33:01 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Apr 10 12:33:06 2014 Subject: [Histonet] RE: competency form In-Reply-To: <20140410103601.KLHN6.214661.imail@fed1rmwml208> References: , <20140410103601.KLHN6.214661.imail@fed1rmwml208> Message-ID: Has some nice tables that summarize things for a good starting point especially if you are building from "scratch" . Thank you for sharing this. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Thu, 10 Apr 2014 10:36:01 -0400 > From: cytch7@cox.net > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] RE: competency form > > I was having the same problems with developing competency records for my cytotechnologist. I found this article from ADVACE for Adminstrators to be very helpful in creating my competency forms. We just had an on-site CAP inspection and it seemed to satisfy them. Hope this link is helpful. The article's author is Teresa Scott. > > http://laboratory-manager.advanceweb.com/Web-Extras/Online-Extras/Competency-Assessment.aspx > > Valerie Biendara SCT(ASCP)IAC > Cytology Supervisor > NWA Pathology Associates > > > ---- "Demarinis wrote: > > ============= > I would be interested in reviewing the competency form. Could you email it to me as well? Thanks. > Carolyn - cdemarinis@saratogacare.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie > Sent: Tuesday, April 08, 2014 5:18 PM > To: Houston, Ronald; Histonet > Subject: [Histonet] RE: competency form > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > Thanks, > Amber > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald > Sent: Tuesday, April 08, 2014 2:37 PM > To: Histonet > Subject: [Histonet] competency form > > Can someone please share the competency form(s) they are using to satisfy CAP? > > I am having problems convincing our QA/Compliance folks of the differences between testing in AP compared to the other lab disciplines, who do read our test results > > Thanks > > Ronnie Houston, MS HT(ASCP)QIHC > Anatomic Pathology Manager > ChildLab, a Division of Nationwide Children's Hospital www.childlab.com > > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.houston@nationwidechildrens.org > www.NationwideChildrens.org > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail communication and any attachments may contain > confidential and privileged information for the use of the > designated recipients named above. > If you are not the intended recipient, you are hereby notified > that you have received this communication in error and that > any review, disclosure, dissemination, distribution or copying > of it or its contents is prohibited. If you have received this > communication in error, please notify Saratoga Hospital > immediately by e-mail at privacy@saratogacare.org and > destroy all copies of this communication and any attachments. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.LaFriniere <@t> ccplab.com Thu Apr 10 12:55:37 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Thu Apr 10 12:56:45 2014 Subject: [Histonet] RE: CPT codes In-Reply-To: <5A33C952BB67F4468AF1F36D739212BC011247EA28@JERRY.Gia.com> References: <20140409162027.B92F41E8079@trendmess-svr.holyredeemer.local>, <5A33C952BB67F4468AF1F36D739212BC011247EA28@JERRY.Gia.com> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29668EB7@AHCMSASEXCH02.my.ahc.local> Amber Google CPT Standard 2014 many sites have it for sale, I would suggest a new one each year so you can understand the changes which are happen quite frequently in Pathology and will for a while. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, April 09, 2014 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CPT codes Does anyone know where I can find a list of all the special stains/IHC stains with their appropriate CPT code? Is there a book to buy or can I google it and print something off? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdemarinis <@t> SARATOGACARE.ORG Fri Apr 11 06:39:59 2014 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Fri Apr 11 06:40:05 2014 Subject: [Histonet] Meditech - electronic ordering Message-ID: <1C75A843982A7B44BB368A3CC946ABCA0A70E755@SHEXCHMBX01.SARAHOSP.ORG> We have 5.66 Meditech Magic and we need to stop using paper requisitions and implement electronic ordering for anatomic pathology and cytology tests. I do not know where to begin. Can anyone give some insight on this topic? Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From a.prior <@t> tissueregenix.com Fri Apr 11 09:48:12 2014 From: a.prior <@t> tissueregenix.com (Andrew Prior) Date: Fri Apr 11 09:49:17 2014 Subject: [Histonet] Test house needed Message-ID: Hi all, I have been asked to identify test houses in the USA that we can use to outsource some of our histology studies. We will be using human tissue (dermis, tendon, meniscus and heart valves) and require H&E, DAPI, Picrosirius Red and Russell Movat Pentachrome staining. We would require the lab to have all the appropriate licencing, and prefer the lab to be GLP compliant. If you work in, or know of a suitable lab then please drop me a private message (to avoid clogging up Histonet) and I'll get back to you with more specifics. Many thanks Andrew Andrew Prior Histologist Tissue Regenix Group The Biocentre York Science Park Innovation Way Heslington, York YO10 5NY E-mail: a.prior@tissueregenix.com Website: www.tissueregenix.com The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and delete the material from any computer. Although we routinely screen for viruses, addressees should check this e-mail and any attachment for viruses. We make no warranty as to absence of viruses in this e-mail or any attachments. Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 5NY Registered No. 05969271 From joelleweaver <@t> hotmail.com Fri Apr 11 10:14:13 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Apr 11 10:14:18 2014 Subject: [Histonet] RE: competency form- Inspector Perspective. In-Reply-To: References: Message-ID: Thank you- good reminder of what the true goals are. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: ibernard@uab.edu > To: trathborne@somerset-healthcare.com; smah2@msn.com; joelleweaver@hotmail.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: competency form- Inspector Perspective. > Date: Thu, 10 Apr 2014 01:57:10 +0000 > > I recently had the great honor to serve as a CAP inspector in Arizona. The lab we inspected will be accredited. > > Lest we forget as inspectors or future inspectors, I hope the below perspective illuminates the purpose of the CAP inspection and helps preserve the integrity of the program if adhered to. > > As inspectors, we are trained to be thorough but to simultaneously use "objective judgment" i.e. " there may be several ways to meet the intent of the question" and that "we must review all pertinent documentation and then determine if the process meets the intent of the requirement". > > Keeping in mind that the purpose of CAP inspection is not punitive but "quality laboratory improvement to fulfill the regulatory purpose of the inspection (CLIA)". Our focus should be, in the interest of time and thoroughness "major compliance issue rather than nitpick". This helps us maintain "professionalism and preserve the peer-review nature of the program". > > So what is it that constitutes compliance? > Per CAP: > "- One, that the laboratory has defined a policy, a procedure, or a plan "of the three P's" for how they are going to do things in the lab. > - Secondly, actual practices that matches those three P's. > - Finally, documentation to support the fact that practice has indeed matched policies and procedures." > > So what is a deficiency? > > Per CAP: > "A deficiency means that the lab did not meet the intent of the checklist item. It's not the wording; it's not the specifics. It is the intent". If any of the three above criteria are not yet met, we should cite a deficiency" We are admonished to remember that "there may be many ways to accomplish an objective. The lab may not do things exactly the way that your lab does, but may still be meeting the intent of the requirement(s). Citing a laboratory for "not doing it the way we do it" is a common inspector error." > > Per CAP, partial compliance is the following: "If there is partial compliance (e.g., some records are inconsistent, one bottle of reagent was not labeled completely, a few temperatures were not recorded, etc.), you must judge whether the degree of non-compliance is likely to have adverse effects on test accuracy, patient care, or worker safety. Also, determine if the lab staff was aware of the inconsistency and if corrective actions were performed. If adverse effects are likely or if there are definite patterns (e.g., missing temperatures only on weekends) without corrective actions" only then we must cite a deficiency. > > Bottom-line, If you feel you were incorrectly cited since you met the intent of the question, you should appeal to CAP. It is an inspected lab's right. If a phase 11 deficiency, submit your evidence of compliance and the Lab Accreditation Committee will either overrule or sustain. I suspect they will overrule. > > Just saying, but based upon just what you described, it sounds like you all met the intent of the question. > > MSgt Ian R Bernard, HT(ASCP), MSHA-UAB > Anatomic Pathology Lab Manager > USAF- Active Duty > ibernard@uab.edu > ian.bernard@comcast.net > > > > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni > Sent: Wednesday, April 09, 2014 7:04 AM > To: 'Sheila Haas'; joelle weaver; Amber McKenzie; Houston, Ronald; Histonet > Subject: RE: [Histonet] RE: competency form > > CAP inspectors may have opinions which differ from our own, and their interpretation of standards may also be different. Have you challenged this deficiency with CAP? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas > Sent: Wednesday, April 09, 2014 8:52 AM > To: joelle weaver; Amber McKenzie; Houston, Ronald; Histonet > Subject: RE: [Histonet] RE: competency form > > What we had here, which did not meet the CAP inspectors requirements apparently, were the procedure (of course); a form with each observation of each task documented along with any corrective action necessary; the correlation of proficiency tests, educational assessments and performance reviews for technical staff; daily evaluations from the pathologists concerning staining, microtomy and grossing; and educational training documentation. We had no idea with all pieces of this documentation that we were anticipated to have more. The form for DO of each task was not detailed enough (despite listing each task and proficiency or corrective action of each task) according to the inspectors. I was hoping someone could share a form so as to assist us in seeing what holes there are in our form. While this DO form is definitely not our only form, the inspectors specifically commented on this one. > > If anyone can assist, it would be helpful. > Thank you, > Sheila Haas > MicroPath Laboratories, Inc. > Quality Assurance Coordinator > > > From: joelleweaver@hotmail.com > To: smah2@msn.com; amber.mckenzie@gastrodocs.net; ronald.houston@nationwidechildrens.org; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: competency form > Date: Wed, 9 Apr 2014 12:26:47 +0000 > > > > > > Is the question that Ronnie posed yesterday requesting justification of the need and extent of competency documentation for histology, or is it just a form needed? The general checklist pretty much sums up the necessity for doing, and required elements to me- GEN. 55500 and CLIA. Maybe I am not understanding? > As for a form, I would expect that the specific items on any forms will vary by your personnel and by the testing and processes you perform. > What I did to document initial training and competency was a make a summary checklist for each "bench" with tasks and direct observations "DO" for initial training & documentation of satisfactory performance before patient testing. > I just put all those checklists together in a summary table for each person. High complexity; such as grossing, IHC, FISH scoring get more attention and documentation, the waived tests, you have more discretion,- but I thought it easier to do everything about the same. I have not been inspected on this document yet ( so can't say if CAP will have issues with it- but will know soon...) but here is basically what I did to meet GEN.55500 or the main parts; > > > Defined how competency is monitored- method and frequency ( just included as part of the competency SOP) Orientation and initial training documentation in a checklist for general lab, safety Training checklist on each technical bench, instrument, major procedure PT records and performance/results > DO- a practical assessment ( block, slides, stains), for the assessment of previously analyzed specimens, and a PI feedback checklist for the technical from this audit of issues- how/what to improve Check off in performing QC, calibration, patient ID procedures ( acceptable error rates), examples for file DO of grossing, other performance such as instrument programming/maintenance Written quiz, policies & procedures, troubleshooting( problem solving documentation) Continuing education participation records > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > From: smah2@msn.com > > To: amber.mckenzie@gastrodocs.net; > > ronald.houston@nationwidechildrens.org; > > histonet@lists.utsouthwestern.edu > > Date: Wed, 9 Apr 2014 11:10:18 +0000 > > Subject: RE: [Histonet] RE: competency form > > CC: > > > > We were recently dinged by CAP for our competency assessments in all areas. While that's no longer my immediate responsibility, I would love to be able to assist the lab manager with some information so we can tweek our assessments if you all wouldn't mind sharing with me as well. > > Thanks a bunch. > > Sheila Haas > > MicroPath Laboratories, Inc. > > Quality Assurance Coordinator > > > > > From: amber.mckenzie@gastrodocs.net > > > To: Ronald.Houston@nationwidechildrens.org; > > > histonet@lists.utsouthwestern.edu > > > Date: Tue, 8 Apr 2014 21:17:44 +0000 > > > CC: > > > Subject: [Histonet] RE: competency form > > > > > > Can you pass it on to me as well? I'd love to compare what I've got to what someone else is doing. > > > Thanks, > > > Amber > > > > > > -----Original Message----- > > > From: histonet-bounces@lists.utsouthwestern.edu > > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > > Houston, Ronald > > > Sent: Tuesday, April 08, 2014 2:37 PM > > > To: Histonet > > > Subject: [Histonet] competency form > > > > > > Can someone please share the competency form(s) they are using to satisfy CAP? > > > > > > I am having problems convincing our QA/Compliance folks of the > > > differences between testing in AP compared to the other lab > > > disciplines, who do read our test results > > > > > > Thanks > > > > > > Ronnie Houston, MS HT(ASCP)QIHC > > > Anatomic Pathology Manager > > > ChildLab, a Division of Nationwide Children's Hospital > > > www.childlab.com > > > > > > 700 Children's Drive > > > Columbus, OH 43205 > > > (P) 614-722-5450 > > > (F) 614-722-2899 > > > ronald.houston@nationwidechildrens.org > > idechildrens.org> > > > www.NationwideChildrens.org > > > > > > "One person with passion is better than forty people merely interested." > > > ~ E.M. Forster > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jwell1958 <@t> msn.com Fri Apr 11 13:58:32 2014 From: jwell1958 <@t> msn.com (Janci Wellborn) Date: Fri Apr 11 13:58:37 2014 Subject: [Histonet] 37th Annual Missouri Spring Symposium Message-ID: Good Afternoon, The Missouri Society for Histotechnology has partnered with the NSH to offer an exciting two day event in America?s heartland. The two day event offers general sessions, workshops and exhibitors for one low price. Attendees have the option to register for a one or two day pass and techs studying for the HT exam have an opportunity to attend an HT prep course. Join us at the gorgeous Chateau on the Lake in Branson for great education and networking! Register at https://s3.goeshow.com/nsh/2014MSS/ereg403598.cfm?clear Hope to see you there! Janci Wellborn, HTL (ASCP) From akelley <@t> path.wustl.edu Fri Apr 11 14:34:17 2014 From: akelley <@t> path.wustl.edu (Kelley, Amanda) Date: Fri Apr 11 14:34:23 2014 Subject: [Histonet] 37th Annual Missouri Spring Symposium In-Reply-To: References: Message-ID: Please be aware that the room rate for the Symposium is no longer available you will have to pay full price. Amanda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janci Wellborn Sent: Friday, April 11, 2014 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 37th Annual Missouri Spring Symposium Good Afternoon, The Missouri Society for Histotechnology has partnered with the NSH to offer an exciting two day event in America's heartland. The two day event offers general sessions, workshops and exhibitors for one low price. Attendees have the option to register for a one or two day pass and techs studying for the HT exam have an opportunity to attend an HT prep course. Join us at the gorgeous Chateau on the Lake in Branson for great education and networking! Register at https://s3.goeshow.com/nsh/2014MSS/ereg403598.cfm?clear Hope to see you there! Janci Wellborn, HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From KSimeone <@t> leavittmgt.com Fri Apr 11 15:01:46 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Fri Apr 11 15:01:51 2014 Subject: [Histonet] white Sakura ice trays Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C01972E@vm-email.leavittmgt.com> Can anyone tell me what vendor I can purchase the white Sakura ice trays for blocks that you can freeze (liquid inside) and put blocks on when cutting. Hope this run on sentence makes sense! Thanks in advance. :-) Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From akelley <@t> path.wustl.edu Fri Apr 11 15:10:19 2014 From: akelley <@t> path.wustl.edu (Kelley, Amanda) Date: Fri Apr 11 15:10:26 2014 Subject: [Histonet] 37th Annual Missouri Spring Symposium In-Reply-To: References: Message-ID: Hint: It is cheaper to book your room at the Chateau on the Lake online than over the phone for the MSH/NSH meeting. Once you let the hotel know that you are with the group they are suddenly no longer giving the $129.00 rate for Friday. Do not call book on line at their website. The block of rooms are gone for the meeting anyway, and you can't get the rate. So book ONLINE at http://chateauonthelake1-px.trvlclick.com/accommodations/index.cfm Amanda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelley, Amanda Sent: Friday, April 11, 2014 2:34 PM To: Janci Wellborn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] 37th Annual Missouri Spring Symposium Please be aware that the room rate for the Symposium is no longer available you will have to pay full price. Amanda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janci Wellborn Sent: Friday, April 11, 2014 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 37th Annual Missouri Spring Symposium Good Afternoon, The Missouri Society for Histotechnology has partnered with the NSH to offer an exciting two day event in America's heartland. The two day event offers general sessions, workshops and exhibitors for one low price. Attendees have the option to register for a one or two day pass and techs studying for the HT exam have an opportunity to attend an HT prep course. Join us at the gorgeous Chateau on the Lake in Branson for great education and networking! Register at https://s3.goeshow.com/nsh/2014MSS/ereg403598.cfm?clear Hope to see you there! Janci Wellborn, HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Apr 11 16:31:29 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Apr 11 16:31:28 2014 Subject: [Histonet] white Sakura ice trays In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C01972E@vm-email.leavittmgt.com> Message-ID: <924E5881-E3DF-414B-999E-C314D4798177@mtsac.edu> We purchased ours directly from Sakura. Sent from my iPad > On Apr 11, 2014, at 1:04 PM, "Delray Beach Pathology Kari Simeone" wrote: > > Can anyone tell me what vendor I can purchase the white Sakura ice trays for blocks that you can freeze (liquid inside) and put blocks on when cutting. Hope this run on sentence makes sense! Thanks in advance. :-) > > > > Kari M Simeone > > Histology/Immunohistochemistry Specialist Supervisor > > Alternate Laboratory Supervisor > > Delray Beach Technical Laboratory > > ksimeone@leavittmgt.com > > > > > > The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren <@t> gmail.com Fri Apr 11 16:32:12 2014 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Fri Apr 11 16:32:18 2014 Subject: [Histonet] Test house needed In-Reply-To: References: Message-ID: Isn't it more difficult to read left handed American slides on your right handed English microscopes? Sincerely? Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Apr 11, 2014 at 9:48 AM, Andrew Prior wrote: > Hi all, > > I have been asked to identify test houses in the USA that we can use to > outsource some of our histology studies. > We will be using human tissue (dermis, tendon, meniscus and heart valves) > and require H&E, DAPI, Picrosirius Red and Russell Movat Pentachrome > staining. We would require the lab to have all the appropriate licencing, > and prefer the lab to be GLP compliant. > If you work in, or know of a suitable lab then please drop me a private > message (to avoid clogging up Histonet) and I'll get back to you with more > specifics. > > Many thanks > > Andrew > > Andrew Prior > Histologist > Tissue Regenix Group > The Biocentre > York Science Park > Innovation Way > Heslington, York > YO10 5NY > E-mail: a.prior@tissueregenix.com > Website: www.tissueregenix.com > > The information transmitted is intended only for the person or entity to > which it is addressed and may contain confidential and/or privileged > material. Any review, retransmission, dissemination or other use of, or > taking of any action in reliance upon, this information by persons or > entities other than the intended recipient is prohibited. If you received > this in error, please contact the sender and delete the material from any > computer. Although we routinely screen for viruses, addressees should check > this e-mail and any attachment for viruses. We make no warranty as to > absence of viruses in this e-mail or any attachments. > > Registered Office: The Biocentre, Innovation Way, Heslington, York, YO10 > 5NY > Registered No. 05969271 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hrfulklab <@t> gmail.com Sat Apr 12 20:41:45 2014 From: hrfulklab <@t> gmail.com (H R) Date: Sat Apr 12 20:41:49 2014 Subject: [Histonet] Problems with processors and dehydration Message-ID: I have had some horrible luck with a processor that was purchased new 3 years ago, I won't mention the name. It worked great for 2.5 years, perfect specimens coming off. Then problems started with clogged lines , long drains skipped stations, etc. The company took it back to "work"on it b/c they couldn't figure out what was wrong, they gave me a loaner of the same kind, used it for a month and the computer went out on it and it spewed paraffin all over inside of machine and lab floor, so the sent me one of their "latest" models to use while they rebuilt my original processor. I used it for a couple of weeks and their was something wrong with the tissue, I couldn't put my finger on it and I thought I just needed to tweek the program. I was running an identical program to my original processor and it wasn't working well. Meanwhile, they send back my original machine, supposedly rebuilt, and want me to use it and make sure it's going to work before they pick up the latest model they were letting me use. I use my original machine for a week, get wonderful results and the clogged lines start again.Go back to the latest model and am having a horrible time, specimens are almost unreadable. Anything bigger than a shave, is horrible. I don't believe it's fixation, they are spongy and puff up on the ice and peel out of section when cut, which makes me believe poor dehydration. I don't know how that can be, I am running an overnight protocol with 2 formalins, 80% alc, 95% alc, 3-100% acl, 3 xylenes 4 paraffins, all of at least an hour a piece. If anything , they should be overprocess! I don't get any alarms on the machine, I am at a loss, the company is at a loss with all of it, I think it's poor equipment all around. Any suggestions? -- From rjbuesa <@t> yahoo.com Sun Apr 13 08:33:58 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Apr 13 08:36:52 2014 Subject: [Histonet] Problems with processors and dehydration In-Reply-To: References: Message-ID: <1397396038.83965.YahooMailNeo@web120402.mail.ne1.yahoo.com> I am also at a loss. The unnamed manufacturer seems to have produced an unreliable machine, except for the initial 2? years it worked fine. I also agree with you that if anything the tissues would be over-processed with your protocol. Now if your whole protocol is the same (including fixation) as when the machine worked correctly you can be sure the problem is in the machine both the initial one and the loaner. My recommendation? Talk with the lab administrator and explain the whole situation and budget for a new tissue processor and count your loses. This?will be better than making a wrong diagnosis to a patient because of a poorly processed tissue?and been found liable for it. You could always start fiddling with reagents and times but I do not think you will solve the problem. Start anew with another issue processor. Ren? J. On Saturday, April 12, 2014 9:43 PM, H R wrote: I have had some horrible luck with a processor that was purchased new 3 years ago, I won't mention the name. It worked great for 2.5 years, perfect specimens coming off. Then problems started with clogged lines , long drains skipped stations, etc. The company took it back to "work"on it b/c they couldn't figure out what was wrong, they gave me a loaner of the same kind, used it for a month and the computer went out on it and it spewed paraffin all over inside of machine and lab floor, so the sent me one of their "latest" models to use while they rebuilt my original processor. ? I used it for a couple of weeks and their was something wrong with the tissue, I couldn't put my finger on it and I thought I just needed to tweek the program. I was running an identical program to my original processor and it wasn't working well. Meanwhile, they send back my original machine, supposedly rebuilt, and want me to use it and make sure it's going to work before they pick up the latest model they were letting me use. I use my original machine for a week, get wonderful results and the clogged lines start again.Go back to the latest model and am having a horrible time, specimens are almost unreadable. Anything bigger than a shave, is horrible. ? I don't believe it's fixation, they are spongy and puff up on the ice and peel out of section when cut, which makes me believe poor dehydration. I don't know how that can be, I am running an overnight protocol with 2 formalins, 80% alc, 95% alc, 3-100% acl, 3 xylenes 4 paraffins, all of at least an hour a piece. If anything , they should be overprocess! I don't get any alarms on the machine, I am at a loss, the company is at a loss with all of it, I think it's poor equipment all around. Any suggestions? -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood <@t> health.nsw.gov.au Sun Apr 13 19:01:12 2014 From: tony.henwood <@t> health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun Apr 13 19:01:32 2014 Subject: [Histonet] Problems with processors and dehydration In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157E00C0CB1@xmdb04.nch.kids> Hi HR, Does your processor request a dump when the first alcohol reaches a critical level? If yes, make sure that you are only using aqueous solutions in your fixative stations (otherwise, if the last "fixative" station is an ethanol solution eg FAA, then the critical specific gravity won't be reached - though from what you have stated this is not the case) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R Sent: Sunday, 13 April 2014 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with processors and dehydration I have had some horrible luck with a processor that was purchased new 3 years ago, I won't mention the name. It worked great for 2.5 years, perfect specimens coming off. Then problems started with clogged lines , long drains skipped stations, etc. The company took it back to "work"on it b/c they couldn't figure out what was wrong, they gave me a loaner of the same kind, used it for a month and the computer went out on it and it spewed paraffin all over inside of machine and lab floor, so the sent me one of their "latest" models to use while they rebuilt my original processor. I used it for a couple of weeks and their was something wrong with the tissue, I couldn't put my finger on it and I thought I just needed to tweek the program. I was running an identical program to my original processor and it wasn't working well. Meanwhile, they send back my original machine, supposedly rebuilt, and want me to use it and make sure it's going to work before they pick up the latest model they were letting me use. I use my original machine for a week, get wonderful results and the clogged lines start again.Go back to the latest model and am having a horrible time, specimens are almost unreadable. Anything bigger than a shave, is horrible. I don't believe it's fixation, they are spongy and puff up on the ice and peel out of section when cut, which makes me believe poor dehydration. I don't know how that can be, I am running an overnight protocol with 2 formalins, 80% alc, 95% alc, 3-100% acl, 3 xylenes 4 paraffins, all of at least an hour a piece. If anything , they should be overprocess! I don't get any alarms on the machine, I am at a loss, the company is at a loss with all of it, I think it's poor equipment all around. Any suggestions? -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From richard.wild <@t> wanadoo.fr Mon Apr 14 04:56:52 2014 From: richard.wild <@t> wanadoo.fr (richard wild) Date: Mon Apr 14 04:57:00 2014 Subject: [Histonet] Sakura DRS 601 - where is the battery ? Message-ID: <534BB0E4.80801@wanadoo.fr> Hello dear Histoneters I have got a "low voltage battery" message for the DRS 601 I would like to change the battery myself but I dont know where it is I openned the front pannel (they are two small cards ... without battery) I openned the right side pannel (they are two large horizontal cards that can be easily moved out ... without battery) So does anyone know where are that battery (and wich type of battery it is ?) Thanks for friendly advice R W Bonjour Je me permets de vous poser une question concernant le 601 J'ai un message de "low voltage battery" pour le DRS 601 par contre je n'ai pas r?ussi ? localiser la batterie ? changer : Elle ne semble pas ?tre sur les 2 petites cartes verticales sous le panneau frontal droit Elle ne semble pas ?tre sur les 2 grandes cartes horizontales que l'on peut faire sortir par le capot lat?rale droit O? se trouve donc cette batterie ? Cordialement R w From GoetzF <@t> si.edu Mon Apr 14 09:50:18 2014 From: GoetzF <@t> si.edu (Goetz, Freya E.) Date: Mon Apr 14 09:50:26 2014 Subject: [Histonet] Ferric ammonium sulfate Message-ID: <43BF6F1B-0FC6-48ED-86D4-8AC187668CD3@si.edu> Good morning everyone! I was wondering if anyone has an opinion on where to buy ferric ammonium sulfate. I want to use it as a mordant for Heidenhain's hematoxylin and I want to buy the solid chemical. I looked for the options on Sigma and the price varies tenfold between options. Most of them are 99% pure so I am wondering what I should be looking for in a mordant quality chemical and what companies are the best for histological reagents. Thank you for your time! Freya Museum Technician Department of Invertebrate Zoology Smithsonian National Museum of Natural History Washington, DC From rjbuesa <@t> yahoo.com Mon Apr 14 10:18:27 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 14 10:21:31 2014 Subject: [Histonet] Ferric ammonium sulfate In-Reply-To: <43BF6F1B-0FC6-48ED-86D4-8AC187668CD3@si.edu> References: <43BF6F1B-0FC6-48ED-86D4-8AC187668CD3@si.edu> Message-ID: <1397488707.72697.YahooMailNeo@web120404.mail.ne1.yahoo.com> Ideally, you should always use a chemical in its highest purity grade BUT this refers to analytical chemistry procedures were you are "looking for something chemically" and do not want to introduce any impurities. To be used as a "mordant" in the always "chemically obscure" histology procedures, I think you are fine with a less "chemically pure" ferric ammonium sulfate that will always absorb water changing the "purity" state of the original. Ren? J.? On Monday, April 14, 2014 10:51 AM, "Goetz, Freya E." wrote: Good morning everyone! I was wondering if anyone has an opinion on where to buy ferric ammonium sulfate. I want to use it as a mordant for Heidenhain's hematoxylin and I want to buy the solid chemical. I looked for the options on Sigma and the price varies tenfold between options. Most of them are 99% pure so I am wondering what I should be looking for in a mordant quality chemical and what companies are the best for histological reagents. Thank you for your time! Freya Museum Technician Department of Invertebrate Zoology Smithsonian National Museum of Natural History Washington, DC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Mon Apr 14 10:41:58 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Apr 14 10:42:04 2014 Subject: [Histonet] Awards Spotlight - Jules Elias Excellence in Immunohistochemistry Award Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E058B9@SBS2K8.premierlab.local> Awards Spotlight - Jules Elias Excellence in Immunohistochemistry Award This award is sponsored by Dr. Richard W. Cartun with a $1000 grant to the recipient. This award is presented annually to an NSH member that is currently utilizing immunohistochemisty in a clinical and or research setting and is nominated by his/her peers for excellence in immunohistochemisty techniques. Chris van der Loos received this award in 2010. I'm sure most of you are aware that Chris passed last year, but his legacy lives on. Chris was one of the most influential people in developing and mastering the science of multiple IHC staining. Chris was without doubt the acknowledged world's expert in multicolor IHC staining methods. If you know of an individual who strives to excel in immunohistochemistry, who is willing share their knowledge then nominate them for this award. http://nsh.org/scholarships-awards/education/individual%20awards?nid=1402 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From Karen.Heckford <@t> DignityHealth.org Mon Apr 14 13:09:55 2014 From: Karen.Heckford <@t> DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Mon Apr 14 13:10:07 2014 Subject: [Histonet] Pathnet Message-ID: Does anyone in the local San Francisco Bay Area use the Cerner Pathnet program. I need some help!!! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From joelleweaver <@t> hotmail.com Mon Apr 14 13:29:05 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Apr 14 13:29:09 2014 Subject: [Histonet] Problems with processors and dehydration In-Reply-To: References: Message-ID: sounds like you have been through the "drill" with this one. Did you already test the solutions with a hydrometer & carry over contamination? If the lines are clogging, I would guess things must be co-mingling, intermixing and being flushed back into another station. If it skips stations, well no wonder the tissue is not processing. I wish I could see the tissue ( and instrument), but your description of the tissue when sectioning, makes me wonder if you have carry over of water in the graduated alcohols. Does the tissue float in the molten paraffin with little air bubbles sometimes if gently pressed? No dehydration , no infiltration, will be spongy and pop or almost peel out. Did they tell you what was sitting in the lines during rebuild? I would just replace this thing- it is not worth losing patient tissue if it cannot be corrected after all this. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Sat, 12 Apr 2014 21:41:45 -0400 > From: hrfulklab@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Problems with processors and dehydration > > I have had some horrible luck with a processor that was purchased new 3 > years ago, I won't mention the name. It worked great for 2.5 years, perfect > specimens coming off. Then problems started with clogged lines , long > drains skipped stations, etc. The company took it back to "work"on it b/c > they couldn't figure out what was wrong, they gave me a loaner of the same > kind, used it for a month and the computer went out on it and it spewed > paraffin all over inside of machine and lab floor, so the sent me one of > their "latest" models to use while they rebuilt my original processor. > I used it for a couple of weeks and their was something wrong with the > tissue, I couldn't put my finger on it and I thought I just needed to tweek > the program. I was running an identical program to my original processor > and it wasn't working well. Meanwhile, they send back my original machine, > supposedly rebuilt, and want me to use it and make sure it's going to work > before they pick up the latest model they were letting me use. I use my > original machine for a week, get wonderful results and the clogged lines > start again.Go back to the latest model and am having a horrible time, > specimens are almost unreadable. Anything bigger than a shave, is horrible. > I don't believe it's fixation, they are spongy and puff up on the ice and > peel out of section when cut, which makes me believe poor dehydration. I > don't know how that can be, I am running an overnight protocol with 2 > formalins, 80% alc, 95% alc, 3-100% acl, 3 xylenes 4 paraffins, all of at > least an hour a piece. If anything , they should be overprocess! I don't > get any alarms on the machine, I am at a loss, the company is at a loss > with all of it, I think it's poor equipment all around. Any suggestions? > -- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Millard <@t> prlnet.com Mon Apr 14 18:03:15 2014 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Mon Apr 14 18:03:22 2014 Subject: [Histonet] Re: Problems with processors and dehydration Message-ID: <64D11CD058311340A1966C234C6B0B893038685C@PRLEXCH02.prlnet.com> It does sound like your processor is faulty, but the culprit may be contaminated lines. If you were skipping stations, having clogged lines, this could have occurred. We had an issue where a tech forgot to drain the retort after a warm-water flush, but refilled the empty container. When the next person loading the processor saw the retort was full and drained it (without seeing the bottle was no longer empty), we backed up into other lines. I initially only cleaned the lines that I thought were effected and refilled and checked all of the bottles using a hydrometer, and then had a processing issue similar to what you are describing-underprocessed. We did a complete change of reagents and still had the issue. I had to thouroughly clean all of the alcohol and xylene lines with 100% alcohol to fix the problem. If the processor is faulty and causing contamination, cleaning the lines once isn't going to fix the problem, but it's a possible explanation, especially if the last alcohol or the xylenes are contaminated with any water. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From Richard.Cartun <@t> hhchealth.org Mon Apr 14 18:03:37 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Mon Apr 14 18:03:44 2014 Subject: [Histonet] Negative Reagent Control in Diagnostic IHC Testing Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E022AACE4@HHCEXCHMB03.hhcsystem.org> For those of you who are interested, I have an Editorial in the March issue (Volume 22, Number 3) of Applied Immunohistochemistry & Molecular Morphology titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do We Need Them? An Evidence-based Recommendation for Laboratories Throughout the World". You can download a "Free" PDF copy using the Journal's website. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From joelleweaver <@t> hotmail.com Mon Apr 14 18:12:21 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Mon Apr 14 18:12:27 2014 Subject: [Histonet] Negative Reagent Control in Diagnostic IHC Testing In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E022AACE4@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E022AACE4@HHCEXCHMB03.hhcsystem.org> Message-ID: Fabulous, thanks. This change is on my list for 2014. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Richard.Cartun@hhchealth.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 14 Apr 2014 23:03:37 +0000 > Subject: [Histonet] Negative Reagent Control in Diagnostic IHC Testing > > For those of you who are interested, I have an Editorial in the March issue (Volume 22, Number 3) of Applied Immunohistochemistry & Molecular Morphology titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do We Need Them? An Evidence-based Recommendation for Laboratories Throughout the World". You can download a "Free" PDF copy using the Journal's website. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 Fax > richard.cartun@hhchealth.org > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Mon Apr 14 18:50:19 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon Apr 14 18:50:52 2014 Subject: =?utf-8?Q?Re:_[Histonet]_Negative_Reagent_Control_in_Diagnostic_IHC_Testi?= =?utf-8?Q?ng?= Message-ID: Thanks for the great information. Downloaded and being shared w/ my lab. Sent from Windows Mail From: Cartun, Richard Sent: ?Monday?, ?April? ?14?, ?2014 ?4?:?04? ?PM To: histonet For those of you who are interested, I have an Editorial in the March issue (Volume 22, Number 3) of Applied Immunohistochemistry & Molecular Morphology titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do We Need Them? An Evidence-based Recommendation for Laboratories Throughout the World". You can download a "Free" PDF copy using the Journal's website. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Tue Apr 15 08:54:50 2014 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Apr 15 08:54:55 2014 Subject: [Histonet] rare testing Message-ID: Looking for a lab(s) that can perform any or all of the following: IHC for nuclear estradiol Beta R, MYC-N, VDR (vitamin D receptor), PDGFR, EGFR, VEGFR, IL-4R Gene expression testing: MET, IGF, BAX (to determine sensitivity to Adriamycin and doxorubicin) and p53 Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From jpiche <@t> wtbyhosp.org Tue Apr 15 10:33:11 2014 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Tue Apr 15 10:33:22 2014 Subject: [Histonet] Xylene testing ANP 08216 Message-ID: <631955447A364B45B9458D2905635110AC107856@WIN08-MBX-01.wtbyhosp.org> Hey Everyone, Just curious what people are doing with xylene testing? Are people really only doing the initial testing and never testing again if all was good? Seems like there should be periodic testing after decades have gone by! Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From liz <@t> premierlab.com Tue Apr 15 10:40:57 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 15 10:41:04 2014 Subject: [Histonet] RE: Xylene testing ANP 08216 In-Reply-To: <631955447A364B45B9458D2905635110AC107856@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110AC107856@WIN08-MBX-01.wtbyhosp.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E058D8@SBS2K8.premierlab.local> We test every year. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 15, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene testing ANP 08216 Hey Everyone, Just curious what people are doing with xylene testing? Are people really only doing the initial testing and never testing again if all was good? Seems like there should be periodic testing after decades have gone by! Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Tue Apr 15 10:46:02 2014 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Apr 15 10:46:06 2014 Subject: [Histonet] RE: Xylene testing ANP 08216 In-Reply-To: <631955447A364B45B9458D2905635110AC107856@WIN08-MBX-01.wtbyhosp.org> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB750@isexstore03> We test every six months. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 15, 2014 11:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene testing ANP 08216 Hey Everyone, Just curious what people are doing with xylene testing? Are people really only doing the initial testing and never testing again if all was good? Seems like there should be periodic testing after decades have gone by! Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From wbenton <@t> cua.md Tue Apr 15 10:57:46 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Apr 15 10:58:05 2014 Subject: [Histonet] RE: Xylene testing ANP 08216 In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3232B4BB750@isexstore03> References: <631955447A364B45B9458D2905635110AC107856@WIN08-MBX-01.wtbyhosp.org>, <450B7A81EDA0C54E97C53D60F00776C3232B4BB750@isexstore03> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF6B5@CUAEXH1.GCU-MD.local> Annually Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [Valerie.Hannen@parrishmed.com] Sent: Tuesday, April 15, 2014 11:46 AM To: 'Piche, Jessica'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Xylene testing ANP 08216 We test every six months. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.hannen@parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 15, 2014 11:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene testing ANP 08216 Hey Everyone, Just curious what people are doing with xylene testing? Are people really only doing the initial testing and never testing again if all was good? Seems like there should be periodic testing after decades have gone by! Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From jpiche <@t> wtbyhosp.org Tue Apr 15 11:06:28 2014 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Tue Apr 15 11:06:33 2014 Subject: [Histonet] ANP08216 Formaldehyde and xylene testing Message-ID: <631955447A364B45B9458D2905635110AC107893@WIN08-MBX-01.wtbyhosp.org> Thanks for all the responses so quickly. I'd also like to know the frequency of formaldehyde testing as well? Thanks to those who included that as well. Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From wbenton <@t> cua.md Tue Apr 15 11:12:32 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Apr 15 11:12:52 2014 Subject: [Histonet] RE: ANP08216 Formaldehyde and xylene testing In-Reply-To: <631955447A364B45B9458D2905635110AC107893@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110AC107893@WIN08-MBX-01.wtbyhosp.org> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF6B6@CUAEXH1.GCU-MD.local> Annually for that as well Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica [jpiche@wtbyhosp.org] Sent: Tuesday, April 15, 2014 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP08216 Formaldehyde and xylene testing Thanks for all the responses so quickly. I'd also like to know the frequency of formaldehyde testing as well? Thanks to those who included that as well. Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From vperez <@t> pathreflab.com Tue Apr 15 11:13:10 2014 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Tue Apr 15 11:13:17 2014 Subject: [Histonet] RE: ANP08216 Formaldehyde and xylene testing In-Reply-To: <631955447A364B45B9458D2905635110AC107893@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110AC107893@WIN08-MBX-01.wtbyhosp.org> Message-ID: We test annually for xylene, formaldehyde, and isopropyl Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 15, 2014 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANP08216 Formaldehyde and xylene testing Thanks for all the responses so quickly. I'd also like to know the frequency of formaldehyde testing as well? Thanks to those who included that as well. Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDuffett <@t> Pathlinelabs.com Tue Apr 15 11:12:45 2014 From: CDuffett <@t> Pathlinelabs.com (Chris Duffett) Date: Tue Apr 15 11:13:19 2014 Subject: [Histonet] ANP08216 Formaldehyde and xylene testing In-Reply-To: <631955447A364B45B9458D2905635110AC107893@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110AC107893@WIN08-MBX-01.wtbyhosp.org> Message-ID: Same annually Sent from my iPhone Chris Duffett Manager, Laboratory Development Pathline/Emerge 845-709-4246 > On Apr 15, 2014, at 12:12 PM, "Piche, Jessica" wrote: > > Thanks for all the responses so quickly. I'd also like to know the frequency of formaldehyde testing as well? Thanks to those who included that as well. > > Jessica Piche, HT(ASCP) > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From Dennis.Hahn <@t> cookchildrens.org Tue Apr 15 11:55:25 2014 From: Dennis.Hahn <@t> cookchildrens.org (Dennis Hahn) Date: Tue Apr 15 11:55:35 2014 Subject: [Histonet] Waste Flammable Info Please Message-ID: I have an inquiry specifically for Texas and/or Children's hospitals: What is your current volume of waste flammables and how do you handle it? Are you recycling, or is it removed by an outside vendor? A combination of the two? Suggestions on recycling instrumentation? Any drawbacks? We currently collect all waste flammable materials into a 55 Gallon drum and it is removed once or twice a month by a contracted vendor. The problem is, we are disposing of so much now that the vendor is requiring the medical center to obtain a high-use license involving the state and the EPA. We have evaluated recycling many years ago and found the process to be slow and sort of cumbersome. I know that the process has improved quite a bit over the years, but what about the tech time, tech exposure, time for recycling, etc.? Have you found it to be highly cost effective? Thanks in advance for any info you can share. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 From patpxs <@t> gmail.com Tue Apr 15 11:58:37 2014 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Apr 15 11:58:45 2014 Subject: [Histonet] IHC imaging systems Message-ID: Hello out there in Histoland, If any of you out there in the clinical environment are using imaging systems to quantify IHC slides, please let me know what system you are using. We are currently using a system that was provided through Dako, but they no longer support it. So time to consider buying a new one. It needs to have an algorithm that allows the system to distinguish between labelled and not labelled cells and the percentage of each. Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From klaus.dern44 <@t> gmail.com Tue Apr 15 12:04:39 2014 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Tue Apr 15 12:04:45 2014 Subject: [Histonet] Leica,Sakura Microtome Rebuilding Message-ID: In reference to Ms. Weems, Joyce K. posting of Dec.17.2013 ( Microtome Service ). I would like to add that this information is specifically about retrofitting the advance mechanism on the following Microtomes which are not supported by the manufacturer anymore with an adjustment feature to elliminate excessive spindle play. ( thick & thin sections ). The Microtomes in Question are. Reichert/Jung: 2030 Leica: RM 2125 Leica: 2030 Biocut Leica/Jung: 2035 Leica: CM-1850 Cryostat Sakura: SRM 200 For Information, please contact. Klaus Dern Phone: 706 635-8840 Fax: 706 635-3074 e.mail: klaus.dern44@gmail.com From abadesuyi <@t> nrh-ok.com Tue Apr 15 12:13:39 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Tue Apr 15 12:13:43 2014 Subject: [Histonet] Cost of Tissue Processor Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B8220CC@MAIL.nrhnt.nrh-ok.com> Hi, How are you guys doing? I hope you are all doing great. Please we are in the market for tissue processor and I will appreciate it, if you guys could give me an idea of how much it cost. We are going to consider all the brands. Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From CDuffett <@t> Pathlinelabs.com Tue Apr 15 12:14:49 2014 From: CDuffett <@t> Pathlinelabs.com (Chris Duffett) Date: Tue Apr 15 12:15:12 2014 Subject: [Histonet] Semen analysis training In-Reply-To: References: Message-ID: Looking for a location in the New York City or northern New Jersey area that might be able to help with training 1or2 techs to so semen analysis for motility and morphology. We know of the Cleveland clinic program but sea a bit too far to send staff. Any insight will be helpful Sent from my iPhone Chris Duffett Manager, Laboratory Development Pathline/Emerge 845-709-4246 > On Apr 15, 2014, at 1:11 PM, "Klaus Dern" wrote: > > In reference to Ms. Weems, Joyce K. posting of Dec.17.2013 ( Microtome > Service ). I would like to add that this information is specifically about > retrofitting the advance mechanism on the following Microtomes which are > not supported by the manufacturer anymore with an adjustment feature to > elliminate excessive spindle play. ( thick & thin sections ). > > The Microtomes in Question are. > > Reichert/Jung: 2030 > Leica: RM 2125 > Leica: 2030 Biocut > Leica/Jung: 2035 > Leica: CM-1850 Cryostat > Sakura: SRM 200 > > For Information, please contact. > > Klaus Dern > > Phone: 706 635-8840 > Fax: 706 635-3074 > e.mail: klaus.dern44@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From liz <@t> premierlab.com Tue Apr 15 12:21:27 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 15 12:21:32 2014 Subject: [Histonet] RE: Waste Flammable Info Please In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E058DC@SBS2K8.premierlab.local> Dennis First of all we are in Colorado. If you dispose of more than 650 gallons a year in waste you are considered a small quantity generator, that amounts to one 55 gallon drum a month. Once you become a SQG this requires being registered with the EPA, additional safety training, every other year on site audits (In Colorado the Colorado Department of Public Health takes care of this) and then the other year a self-certification check list needs to be completed. If you dispose of less than 650 gallons you are considered a conditionally exempt small quantity generator and are not required to be registered with the EPA. Since you are disposing of 1 to 2 - 55 gallon drums a month that would mean that you would need to decrease your waste by at most 660 gallons a year or by 50%. When we recycled only alcohol in 2013 it looked like we still had around 1 - 55 gallon of waste per month. In 2014 We started recycling alcohol, xylene and proper, so far this year we have only had 2 - 55 gallons of waste picked up, that accounts for a 50% decrease in total waste, so it might be possible for you to start recycling and decrease your waste stream by 50%. We have also seen a cost saving in the purchasing of both alcohol and xylene the amount we purchase has decreased I don't have the specifics on the amount I just know we order less often that we used to. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, April 15, 2014 10:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Waste Flammable Info Please I have an inquiry specifically for Texas and/or Children's hospitals: What is your current volume of waste flammables and how do you handle it? Are you recycling, or is it removed by an outside vendor? A combination of the two? Suggestions on recycling instrumentation? Any drawbacks? We currently collect all waste flammable materials into a 55 Gallon drum and it is removed once or twice a month by a contracted vendor. The problem is, we are disposing of so much now that the vendor is requiring the medical center to obtain a high-use license involving the state and the EPA. We have evaluated recycling many years ago and found the process to be slow and sort of cumbersome. I know that the process has improved quite a bit over the years, but what about the tech time, tech exposure, time for recycling, etc.? Have you found it to be highly cost effective? Thanks in advance for any info you can share. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Apr 15 12:35:41 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 15 12:35:46 2014 Subject: [Histonet] IHC imaging systems In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E058DD@SBS2K8.premierlab.local> Paula There are many companies that have software that will do this for you. I would check the DPA website - www.digitalpathologyassociation.org. The decision you will make is going to be dependent on software capabilities, cost, if you need it to be 510K cleared, etc. Here is a link to the software vendors - note some of these are pathology system vendors https://digitalpathologyassociation.org/software-vendors Here is a link to the hardware vendors (scanners) some also have software but I'm not sure if you can purchase software only and you need to check if its compatible with your images https://digitalpathologyassociation.org/hardware-vendors Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 15, 2014 10:59 AM To: HistoNet Subject: [Histonet] IHC imaging systems Hello out there in Histoland, If any of you out there in the clinical environment are using imaging systems to quantify IHC slides, please let me know what system you are using. We are currently using a system that was provided through Dako, but they no longer support it. So time to consider buying a new one. It needs to have an algorithm that allows the system to distinguish between labelled and not labelled cells and the percentage of each. Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Apr 15 12:25:46 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Apr 15 12:44:25 2014 Subject: [Histonet] Semen analysis training In-Reply-To: References: Message-ID: I would contact any clinical lab in your area. When I worked in the clinical lab we did semen analysis in Hematology. Jennifer From: Chris Duffett To: Cc: "histonet@lists.utsouthwestern.edu" Date: 04/15/2014 10:18 AM Subject: [Histonet] Semen analysis training Sent by: histonet-bounces@lists.utsouthwestern.edu Looking for a location in the New York City or northern New Jersey area that might be able to help with training 1or2 techs to so semen analysis for motility and morphology. We know of the Cleveland clinic program but sea a bit too far to send staff. Any insight will be helpful Sent from my iPhone Chris Duffett Manager, Laboratory Development Pathline/Emerge 845-709-4246 > On Apr 15, 2014, at 1:11 PM, "Klaus Dern" wrote: > > In reference to Ms. Weems, Joyce K. posting of Dec.17.2013 ( Microtome > Service ). I would like to add that this information is specifically about > retrofitting the advance mechanism on the following Microtomes which are > not supported by the manufacturer anymore with an adjustment feature to > elliminate excessive spindle play. ( thick & thin sections ). > > The Microtomes in Question are. > > Reichert/Jung: 2030 > Leica: RM 2125 > Leica: 2030 Biocut > Leica/Jung: 2035 > Leica: CM-1850 Cryostat > Sakura: SRM 200 > > For Information, please contact. > > Klaus Dern > > Phone: 706 635-8840 > Fax: 706 635-3074 > e.mail: klaus.dern44@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Tue Apr 15 12:59:50 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Apr 15 12:59:55 2014 Subject: [Histonet] Waste Flammable Info Please Message-ID: <9F3CFEE76E51B64991C7485270890B404989DF98@EX5.lj.gnf.org> Hi Dennis, I don't think that recycling will fix your EPA license issue. They care about how much you GENERATE through use, not whether it is actually being disposed of or even recycled. We had an issue with the EPA regarding our xylene generation at another facility I used to work in. When I tried to point out that we were recycling it, they said that didn't make a difference. Once it goes into use, it becomes generated waste. You might want to look at changing your practices so that you generate less, i.e. changing the processors according to block numbers or alcohol dilution rather than number of runs, or using smaller containers at the staining bench. Alcohol recycling is great, but you will never get back anything more pure than about 98% so you will need to continue to purchase absolute alcohol. The biggest difficulty is making sure you keep any xylene contaminated alcohol separate and out of the recycler. Xylene will not be removed from alcohol during alcohol recycling so you will still have contaminated alcohol. Anything containing xylene must be recycled as xylene (or manifested out as waste). I hope this helps, Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From BZIMMERM <@t> gru.edu Tue Apr 15 13:08:37 2014 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Apr 15 13:08:46 2014 Subject: [Histonet] HISTOPALOOZA APRIL 25 - 27, 2014 GEORGIA SOCIETY FOR HISTOTECHNOLOGY Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8489D2@EX-MLB-03.ad.georgiahealth.edu> Just a reminder to register by today, if possible. If you are awaiting funds please put that on your registration form. For the last minute people (aka procrastinators) who are filing their taxes today...you can still reserve a cottage/cabin at the Southern Pine Cottages. Call Kit Reames-McClung at 1-800-CALLAWAY and ask for the GHS/HISTOPALOOZA! Rate of $135 per night. We look forward to hosting our Regional "cousins" where we will treat you like family. Our family puts the fun in dysfunctional!! I'm not going to guarantee you'll be able to find a spouse at this family reunion, though. But anything is possible. If you have any questions or need further assistance go to our website and contact any of the GSH BOD. www.histosearch.com/gsh/ From liz <@t> premierlab.com Tue Apr 15 13:16:27 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 15 13:16:34 2014 Subject: [Histonet] RE: Waste Flammable Info Please In-Reply-To: <9F3CFEE76E51B64991C7485270890B404989DF98@EX5.lj.gnf.org> References: <9F3CFEE76E51B64991C7485270890B404989DF98@EX5.lj.gnf.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E058E3@SBS2K8.premierlab.local> Teri That's interesting since we need to fill out a form every year that tabulates the waste generated and not what we purchase. I thought it was based upon the amount of waste generated. That was not our experience. The state came and audited us once we started disposing of waste at a rate of 55 gallons per month. That pushed us past that 650 gallons for a conditionally exempt small quantity generator and therefore we became a small quantity generator. They never asked us how much we purchased only what the waste stream was. Just my experience. Since the states monitor for this, it may vary from State to State how the EPA regs are interpreted or if State laws override federal regarding waste and classifications. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Tuesday, April 15, 2014 12:00 PM To: 'Dennis.Hahn@cookchildrens.org' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Flammable Info Please Hi Dennis, I don't think that recycling will fix your EPA license issue. They care about how much you GENERATE through use, not whether it is actually being disposed of or even recycled. We had an issue with the EPA regarding our xylene generation at another facility I used to work in. When I tried to point out that we were recycling it, they said that didn't make a difference. Once it goes into use, it becomes generated waste. You might want to look at changing your practices so that you generate less, i.e. changing the processors according to block numbers or alcohol dilution rather than number of runs, or using smaller containers at the staining bench. Alcohol recycling is great, but you will never get back anything more pure than about 98% so you will need to continue to purchase absolute alcohol. The biggest difficulty is making sure you keep any xylene contaminated alcohol separate and out of the recycler. Xylene will not be removed from alcohol during alcohol recycling so you will still have contaminated alcohol. Anything containing xylene must be recycled as xylene (or manifested out as waste). I hope this helps, Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton <@t> cua.md Tue Apr 15 13:20:26 2014 From: wbenton <@t> cua.md (Walter Benton) Date: Tue Apr 15 13:22:11 2014 Subject: [Histonet] RE: Waste Flammable Info Please In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E058E3@SBS2K8.premierlab.local> References: <9F3CFEE76E51B64991C7485270890B404989DF98@EX5.lj.gnf.org>, <14E2C6176416974295479C64A11CB9AE019C79E058E3@SBS2K8.premierlab.local> Message-ID: <0B8979A204680A42B93A52B486088CD93931ABF6C4@CUAEXH1.GCU-MD.local> I agree. We have to complete an EPA form for waste generated and hauled away, not purchased. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala [liz@premierlab.com] Sent: Tuesday, April 15, 2014 2:16 PM To: Teri Johnson; 'Dennis.Hahn@cookchildrens.org' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Waste Flammable Info Please Teri That's interesting since we need to fill out a form every year that tabulates the waste generated and not what we purchase. I thought it was based upon the amount of waste generated. That was not our experience. The state came and audited us once we started disposing of waste at a rate of 55 gallons per month. That pushed us past that 650 gallons for a conditionally exempt small quantity generator and therefore we became a small quantity generator. They never asked us how much we purchased only what the waste stream was. Just my experience. Since the states monitor for this, it may vary from State to State how the EPA regs are interpreted or if State laws override federal regarding waste and classifications. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Tuesday, April 15, 2014 12:00 PM To: 'Dennis.Hahn@cookchildrens.org' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Flammable Info Please Hi Dennis, I don't think that recycling will fix your EPA license issue. They care about how much you GENERATE through use, not whether it is actually being disposed of or even recycled. We had an issue with the EPA regarding our xylene generation at another facility I used to work in. When I tried to point out that we were recycling it, they said that didn't make a difference. Once it goes into use, it becomes generated waste. You might want to look at changing your practices so that you generate less, i.e. changing the processors according to block numbers or alcohol dilution rather than number of runs, or using smaller containers at the staining bench. Alcohol recycling is great, but you will never get back anything more pure than about 98% so you will need to continue to purchase absolute alcohol. The biggest difficulty is making sure you keep any xylene contaminated alcohol separate and out of the recycler. Xylene will not be removed from alcohol during alcohol recycling so you will still have contaminated alcohol. Anything containing xylene must be recycled as xylene (or manifested out as waste). I hope this helps, Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From TJohnson <@t> gnf.org Tue Apr 15 13:25:42 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Tue Apr 15 13:25:47 2014 Subject: [Histonet] RE: Waste Flammable Info Please In-Reply-To: <0B8979A204680A42B93A52B486088CD93931ABF6C4@CUAEXH1.GCU-MD.local> References: <9F3CFEE76E51B64991C7485270890B404989DF98@EX5.lj.gnf.org>, <14E2C6176416974295479C64A11CB9AE019C79E058E3@SBS2K8.premierlab.local> <0B8979A204680A42B93A52B486088CD93931ABF6C4@CUAEXH1.GCU-MD.local> Message-ID: <9F3CFEE76E51B64991C7485270890B404989E092@EX5.lj.gnf.org> To clarify, we had to account for new solvent once it was put into waste stream, not what we purchased. Also had to account for Still Bottoms that was manifested out from the recycler. In pounds. Not gallons. Don't ask me to clarify further, because I don't understand it well enough to do so. None of what we were asked to provide made any sense to me. ~tj -----Original Message----- From: Walter Benton [mailto:wbenton@cua.md] Sent: Tuesday, April 15, 2014 11:20 AM To: Elizabeth Chlipala; Teri Johnson; 'Dennis.Hahn@cookchildrens.org' Cc: histonet@lists.utsouthwestern.edu Subject: RE: Waste Flammable Info Please I agree. We have to complete an EPA form for waste generated and hauled away, not purchased. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala [liz@premierlab.com] Sent: Tuesday, April 15, 2014 2:16 PM To: Teri Johnson; 'Dennis.Hahn@cookchildrens.org' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Waste Flammable Info Please Teri That's interesting since we need to fill out a form every year that tabulates the waste generated and not what we purchase. I thought it was based upon the amount of waste generated. That was not our experience. The state came and audited us once we started disposing of waste at a rate of 55 gallons per month. That pushed us past that 650 gallons for a conditionally exempt small quantity generator and therefore we became a small quantity generator. They never asked us how much we purchased only what the waste stream was. Just my experience. Since the states monitor for this, it may vary from State to State how the EPA regs are interpreted or if State laws override federal regarding waste and classifications. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Tuesday, April 15, 2014 12:00 PM To: 'Dennis.Hahn@cookchildrens.org' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Flammable Info Please Hi Dennis, I don't think that recycling will fix your EPA license issue. They care about how much you GENERATE through use, not whether it is actually being disposed of or even recycled. We had an issue with the EPA regarding our xylene generation at another facility I used to work in. When I tried to point out that we were recycling it, they said that didn't make a difference. Once it goes into use, it becomes generated waste. You might want to look at changing your practices so that you generate less, i.e. changing the processors according to block numbers or alcohol dilution rather than number of runs, or using smaller containers at the staining bench. Alcohol recycling is great, but you will never get back anything more pure than about 98% so you will need to continue to purchase absolute alcohol. The biggest difficulty is making sure you keep any xylene contaminated alcohol separate and out of the recycler. Xylene will not be removed from alcohol during alcohol recycling so you will still have contaminated alcohol. Anything containing xylene must be recycled as xylene (or manifested out as waste). I hope this helps, Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From WVanTilburg <@t> GENERAL-DATA.com Tue Apr 15 14:17:42 2014 From: WVanTilburg <@t> GENERAL-DATA.com (VanTilburg, Walt) Date: Tue Apr 15 14:18:17 2014 Subject: [Histonet] Retirement Message-ID: I know it is frowned upon for vendors to write to Histonet, but I hope you will indulge me as I have no easy way to say good bye to some great people. I am retiring next Tuesday the 22nd and for the past 11 year I have met many of you in histo land and have enjoyed the help you all have given me. Without fail every time I entered a lab you unselfishly answered my questions and helped me understand the work flow in the AP labs. For the first couple of years I had to write down and Google almost every third word you said, but I started to catch on and you input and our discussions helped to shape the products that we developed. For that I am grateful. I met a lot of people and made a lot a good friends and saying good bye is hard but the truth is you wore me out and I need to take a break. I would list all of the folk who were especially helpful but the list would be really long so this is just a blanket thank you and good bye. Walt Walter Van Tilburg Vice President of Business Development General Data Healthcare, Inc. 26500 Bruce Road Bay Village, Ohio 44140 440-823-5495 - Mobile 440-808-8983 - Office 440-808-8995 - Fax WVanTilburg@general-data.com Visit our web site http://www.general-data.com/healthcare/ Great news! Triangle Biomedical Sciences (TBS) is now a division of General Data Healthcare! Visit us online at www.general-data.com/hc and www.trianglebiomedical.com. http://www.general-data.com/news/general-data-acquires-triangle-biomedical-sciences ________________________________ This email may contain confidential General Data Company, Inc. information: any unauthorized or improper disclosure, copying, distribution or use of the contents of this email and attached document(s) is prohibited and may be a criminal offense. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you received this email in error, please reply immediately to the sender & delete this message and the attached document(s) without disclosure. From mike <@t> pathview.com Tue Apr 15 14:28:03 2014 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Apr 15 14:28:16 2014 Subject: [Histonet] Retirement In-Reply-To: References: Message-ID: <00cd01cf58e0$d25b9690$7712c3b0$@pathview.com> Walt we wish you the best. It was great working with you. We'll miss you. You were one of the few who really knew their 'stuff' and who was honest and a pleasure to be around. ..hard qualities to find. Good luck Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VanTilburg, Walt Sent: Tuesday, April 15, 2014 2:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retirement I know it is frowned upon for vendors to write to Histonet, but I hope you will indulge me as I have no easy way to say good bye to some great people. I am retiring next Tuesday the 22nd and for the past 11 year I have met many of you in histo land and have enjoyed the help you all have given me. Without fail every time I entered a lab you unselfishly answered my questions and helped me understand the work flow in the AP labs. For the first couple of years I had to write down and Google almost every third word you said, but I started to catch on and you input and our discussions helped to shape the products that we developed. For that I am grateful. I met a lot of people and made a lot a good friends and saying good bye is hard but the truth is you wore me out and I need to take a break. I would list all of the folk who were especially helpful but the list would be really long so this is just a blanket thank you and good bye. Walt Walter Van Tilburg Vice President of Business Development General Data Healthcare, Inc. 26500 Bruce Road Bay Village, Ohio 44140 440-823-5495 - Mobile 440-808-8983 - Office 440-808-8995 - Fax WVanTilburg@general-data.com Visit our web site http://www.general-data.com/healthcare/ Great news! Triangle Biomedical Sciences (TBS) is now a division of General Data Healthcare! Visit us online at www.general-data.com/hc and www.trianglebiomedical.com. http://www.general-data.com/news/general-data-acquires-triangle-biomedical-s ciences ________________________________ This email may contain confidential General Data Company, Inc. information: any unauthorized or improper disclosure, copying, distribution or use of the contents of this email and attached document(s) is prohibited and may be a criminal offense. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you received this email in error, please reply immediately to the sender & delete this message and the attached document(s) without disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Apr 15 14:39:02 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Apr 15 14:39:07 2014 Subject: [Histonet] RE: Retirement In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E391681F489E9@IBMB7Exchange.digestivespecialists.com> Congratulations on your retirement! May you enjoy your time and remember you're supposed to take a picture of the sunrise on your first day of retirement! And personally I don't think vendors are frowned upon. They are full of wise info. What's frowned upon is blatant advertising. Even then the delete key is on the keyboard. Good Luck! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VanTilburg, Walt Sent: Tuesday, April 15, 2014 3:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retirement I know it is frowned upon for vendors to write to Histonet, but I hope you will indulge me as I have no easy way to say good bye to some great people. I am retiring next Tuesday the 22nd and for the past 11 year I have met many of you in histo land and have enjoyed the help you all have given me. Without fail every time I entered a lab you unselfishly answered my questions and helped me understand the work flow in the AP labs. For the first couple of years I had to write down and Google almost every third word you said, but I started to catch on and you input and our discussions helped to shape the products that we developed. For that I am grateful. I met a lot of people and made a lot a good friends and saying good bye is hard but the truth is you wore me out and I need to take a break. I would list all of the folk who were especially helpful but the list would be really long so this is just a blanket thank you and good bye. Walt Walter Van Tilburg Vice President of Business Development General Data Healthcare, Inc. 26500 Bruce Road Bay Village, Ohio 44140 440-823-5495 - Mobile 440-808-8983 - Office 440-808-8995 - Fax WVanTilburg@general-data.com Visit our web site http://www.general-data.com/healthcare/ Great news! Triangle Biomedical Sciences (TBS) is now a division of General Data Healthcare! Visit us online at www.general-data.com/hc and www.trianglebiomedical.com. http://www.general-data.com/news/general-data-acquires-triangle-biomedical-sciences ________________________________ This email may contain confidential General Data Company, Inc. information: any unauthorized or improper disclosure, copying, distribution or use of the contents of this email and attached document(s) is prohibited and may be a criminal offense. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you received this email in error, please reply immediately to the sender & delete this message and the attached document(s) without disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Tue Apr 15 17:03:20 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue Apr 15 17:03:23 2014 Subject: [Histonet] RE: Retirement In-Reply-To: <5A2BD13465E061429D6455C8D6B40E391681F489E9@IBMB7Exchange.digestivespecialists.com> References: , <5A2BD13465E061429D6455C8D6B40E391681F489E9@IBMB7Exchange.digestivespecialists.com> Message-ID: Yes we all appreciate knowledgable, helpful & honest vendors. We all depend on each other. Never had the pleasure to meet you, but wish you many wonderful retirement years! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: lblazek@digestivespecialists.com > To: WVanTilburg@GENERAL-DATA.com; histonet@lists.utsouthwestern.edu > Date: Tue, 15 Apr 2014 15:39:02 -0400 > CC: > Subject: [Histonet] RE: Retirement > > Congratulations on your retirement! May you enjoy your time and remember you're supposed to take a picture of the sunrise on your first day of retirement! > And personally I don't think vendors are frowned upon. They are full of wise info. What's frowned upon is blatant advertising. Even then the delete key is on the keyboard. > Good Luck! > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > Digestive Specialists, Inc > Phone: (937) 396-2623 > Email: lblazek@digestivespecialists.com > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of VanTilburg, Walt > Sent: Tuesday, April 15, 2014 3:18 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Retirement > > I know it is frowned upon for vendors to write to Histonet, but I hope you will indulge me as I have no easy way to say good bye to some great people. I am retiring next Tuesday the 22nd and for the past 11 year I have met many of you in histo land and have enjoyed the help you all have given me. Without fail every time I entered a lab you unselfishly answered my questions and helped me understand the work flow in the AP labs. For the first couple of years I had to write down and Google almost every third word you said, but I started to catch on and you input and our discussions helped to shape the products that we developed. For that I am grateful. > I met a lot of people and made a lot a good friends and saying good bye is hard but the truth is you wore me out and I need to take a break. I would list all of the folk who were especially helpful but the list would be really long so this is just a blanket thank you and good bye. > > Walt > Walter Van Tilburg > Vice President of Business Development > General Data Healthcare, Inc. > 26500 Bruce Road > Bay Village, Ohio 44140 > > 440-823-5495 - Mobile > 440-808-8983 - Office > 440-808-8995 - Fax > WVanTilburg@general-data.com > Visit our web site http://www.general-data.com/healthcare/ > > Great news! Triangle Biomedical Sciences (TBS) is now a division of General Data Healthcare! > Visit us online at www.general-data.com/hc and www.trianglebiomedical.com. > > http://www.general-data.com/news/general-data-acquires-triangle-biomedical-sciences > > > ________________________________ > This email may contain confidential General Data Company, Inc. information: any unauthorized or improper disclosure, copying, distribution or use of the contents of this email and attached document(s) is prohibited and may be a criminal offense. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you received this email in error, please reply immediately to the sender & delete this message and the attached document(s) without disclosure. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From modz9636 <@t> gmail.com Tue Apr 15 19:59:51 2014 From: modz9636 <@t> gmail.com (M.O.) Date: Tue Apr 15 19:59:56 2014 Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? Message-ID: Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). I have never stained with anything like this, so I ran a test. I deparaffinized, blocked with NGS, incubated overnight at 4C with the diluted biotinylated SNA. On the second day, I used Vector's ABC kit and alkaline phosphatase (red) kit. Once stained, I noticed a lot of background. After looking into the blocking step, would a biotin/avidin blocking step be the correct step instead of a serum because I don't have a secondary? How do I know what needs to be blocked - biotin, avidin or both? Is there a way to do this without a kit and use solutions I may have in my lab? Thank you for your help! Sincerely, Merissa From ecameron1 <@t> midcoasthealth.com Wed Apr 16 08:57:56 2014 From: ecameron1 <@t> midcoasthealth.com (Cameron, Elizabeth) Date: Wed Apr 16 08:58:02 2014 Subject: [Histonet] Paraffin Message-ID: Hello histonetters, I recently made the move back to a hospital lab after being in research, and we are currently using two different parrafins for infiltration and embedding. I would like to change the type of paraffin that we are using (I have one in mind), but I was wondering if there were any advantages to using different paraffins for the different steps and what type of validation would need to be done to make this change. We only have one processor, so a side by side comparison would be very difficult. Any thoughts on the matter would be greatly appreciated. Thank you, Liz From DKBoyd <@t> chs.net Wed Apr 16 09:17:31 2014 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Wed Apr 16 09:18:22 2014 Subject: [Histonet] RE: Paraffin In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB762468719B64B@TN001WEXMBX12.US.chs.net> We use Richard Allen Type 6 paraffin for both infiltration and embedding (16 years) with no adverse effects. To validate you can get the pathologist to give you samples for the same specimens you are running. IE; gallbladder 1 for patient dx and 1 for testing, uterus, appendix, any large specimen that you can spare an extra slice. Run the patient dx specimens one night, change only the paraffin in your processor and embedding center run the test samples during the day while the processor isn't in use for patient specimens. Cut and stain per usual as time permits. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cameron, Elizabeth Sent: Wednesday, April 16, 2014 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Hello histonetters, I recently made the move back to a hospital lab after being in research, and we are currently using two different parrafins for infiltration and embedding. I would like to change the type of paraffin that we are using (I have one in mind), but I was wondering if there were any advantages to using different paraffins for the different steps and what type of validation would need to be done to make this change. We only have one processor, so a side by side comparison would be very difficult. Any thoughts on the matter would be greatly appreciated. Thank you, Liz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From ahc53 <@t> georgetown.edu Wed Apr 16 09:22:34 2014 From: ahc53 <@t> georgetown.edu (Anna Coffey) Date: Wed Apr 16 09:22:40 2014 Subject: [Histonet] Thank you Histonet! Message-ID: I took and passed my HTL exam last weekend and just wanted to say "thank you" to all the wonderful, helpful people who responded to my request for advice a few months ago. I really appreciated your kindness and expertise! If anyone is preparing for their exam now and has questions, I'm happy to help! Thanks again, Anna -- Anna Coffey, MS, HTL(ASCP)CM Senior Histology Technician Department of Oncology Histopathology and Tissue Shared Resource LR-10 Pre-Clinical Sciences Building Lombardi Comprehensive Cancer Center Georgetown University 202-687-7890 ahc53@georgetown.edu From hrfulklab <@t> gmail.com Wed Apr 16 09:33:50 2014 From: hrfulklab <@t> gmail.com (H R) Date: Wed Apr 16 09:33:59 2014 Subject: [Histonet] Processor problems Message-ID: I posted the other day about having problems with my two processors. My newest processor I believe is having an issue with dehydration/infiltration. I have tried everything I know, plus some suggestions from this list. I downloaded their manual for the processor and the suggested program for this processor is 1-formalin, 3-95% alcohol, 3-100% alcohols, 3-xylenes and 3 paraffins. Does that not seem like ALOT of alchol? And starting with 95 right after formalin seems it would lead to problems with the salts from formalin. I've never seen a set up like above, but I guess since my problems are with dehydration, I may try it. It just seems like it would dry it out horribly. -- *Heather* From koellingr <@t> comcast.net Wed Apr 16 11:53:21 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Apr 16 11:53:39 2014 Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? In-Reply-To: References: Message-ID: <1170696186.28490007.1397667201669.JavaMail.root@comcast.net> Hi Merissa, don't know if you got any private idea responses so I'll throw in my opinion.? I would always worry about some of the things you are mentioning and that are standard thoughts regarding biotin block, retrieval, etc in IHC. But I would think about your serum, which I steadfastly avoided with SNA or any lectin I used.?? Lectins look at glyco components and serum (or serum substitutes) can be full of glycoproteins and the target then is the blocking serum for your lectin which can cause bad background.? I did and would use washes, diluents, etc that had NO serum or milk or anything like that in them.? You can make your own, completely free of potentially having?glycoproteins or Vector sells some.? For some lectins (look at a list of target sugars) you maybe can get by with serum or milk and such to block?but many I've found you just can't. Ray (still in, whoever would have guessed, once again rainy Seattle) ----- Original Message ----- From: "M.O." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 15, 2014 5:59:51 PM Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. ?The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). ?I have never stained with anything like this, so I ran a test. I deparaffinized, blocked with NGS, incubated overnight at 4C with the diluted biotinylated SNA. ?On the second day, I used Vector's ABC kit and alkaline phosphatase (red) kit. Once stained, I noticed a lot of background. ?After looking into the blocking step, would a biotin/avidin blocking step be the correct step instead of a serum because I don't have a secondary? ?How do I know what needs to be blocked - biotin, avidin or both? ?Is there a way to do this without a kit and use solutions I may have in my lab? Thank you for your help! Sincerely, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Wed Apr 16 13:15:58 2014 From: JWatson <@t> gnf.org (James Watson) Date: Wed Apr 16 13:16:03 2014 Subject: [Histonet] RE: Staining FFPE with biotinylated SNA - how to block? In-Reply-To: <1170696186.28490007.1397667201669.JavaMail.root@comcast.net> References: <1170696186.28490007.1397667201669.JavaMail.root@comcast.net> Message-ID: With our lectin staining on paraffin embedded tissue we use Avidin/Biotin blocking prior to antibody application. Some protocols we do use HIER before the blocking. One important item is that some lectins require a specific diluent. The dilution can be as high as 1:12,000. Lectins are well known for binding with multiple tissue entities, sometimes you can reduce the staining on unwanted tissue entities by reducing your incubation time or dilution. We do not use serum blocking. For PNA we use: PNA Diluent Calcium Chloride???????..??????? 0.0111 gm. Magnesium Chloride????????????? 0.0203 gm. Manganese Chloride????????????? 0.0125 gm. Distilled Water???????????????.?. 100.0 ml James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Wednesday, April 16, 2014 9:53 AM To: M.O. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hi Merissa, don't know if you got any private idea responses so I'll throw in my opinion.? I would always worry about some of the things you are mentioning and that are standard thoughts regarding biotin block, retrieval, etc in IHC. But I would think about your serum, which I steadfastly avoided with SNA or any lectin I used.?? Lectins look at glyco components and serum (or serum substitutes) can be full of glycoproteins and the target then is the blocking serum for your lectin which can cause bad background.? I did and would use washes, diluents, etc that had NO serum or milk or anything like that in them.? You can make your own, completely free of potentially having?glycoproteins or Vector sells some.? For some lectins (look at a list of target sugars) you maybe can get by with serum or milk and such to block?but many I've found you just can't. Ray (still in, whoever would have guessed, once again rainy Seattle) ----- Original Message ----- From: "M.O." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 15, 2014 5:59:51 PM Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. ?The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). ?I have never stained with anything like this, so I ran a test. I deparaffinized, blocked with NGS, incubated overnight at 4C with the diluted biotinylated SNA. ?On the second day, I used Vector's ABC kit and alkaline phosphatase (red) kit. Once stained, I noticed a lot of background. ?After looking into the blocking step, would a biotin/avidin blocking step be the correct step instead of a serum because I don't have a secondary? ?How do I know what needs to be blocked - biotin, avidin or both? ?Is there a way to do this without a kit and use solutions I may have in my lab? Thank you for your help! Sincerely, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Fawn.Bomar <@t> HalifaxRegional.com Wed Apr 16 13:30:00 2014 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Wed Apr 16 13:30:07 2014 Subject: [Histonet] Tracking control blocks Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B96F5FA5@EXCH-2K10.hrhs.com> Happy Easter everyone!!! I was wondering if anyone had a tracking system that they would be willing to share in regards to how you all are keeping a record of your control blocks for IHC. As of right now, we are cutting a slide and staining it for the pathologist to approve- then keeping the paperwork and slide in files, and putting a label on the block in use with the date of approval. Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From gayle.callis <@t> bresnan.net Wed Apr 16 16:13:57 2014 From: gayle.callis <@t> bresnan.net (gayle callis) Date: Wed Apr 16 16:14:47 2014 Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? In-Reply-To: <1170696186.28490007.1397667201669.JavaMail.root@comcast.net> References: <1170696186.28490007.1397667201669.JavaMail.root@comcast.net> Message-ID: <003801cf59b8$c9509630$5bf1c290$@bresnan.net> Dear Merissa, Ray is correct about using serums with lectins. In fact you really are NOT doing immunostaining unless you are using an antibody made to recognize that lectin e.g. anti-lectin therefore a normal serum block is not needed. Serums in fact contain glycoproteins that bind to lectins, so BSA is a better protein carrier for buffers and diluents if you want to make up your own. We used Jacksons immunoglobulin protease free BSA. Ray gave good advice on blocking techniques. However, if you do anti-Lectin, you would be doing IHC and then blocking is legal. If you want a protocol for lectin IHC, I will be happy to forward via private email. With some lectins for direct staining, you should use the Lectin buffer that has no phosphate ions, as explained by Vector technical services. I also have a recipe for the Lectin buffer if you want it. Avidin/biotin blocking is still needed with biotinylated lectin, particularly is the tissue is known to have endogenous biotin. Be sure to find out if your lectin is sensitive to phosphate ions, or use TBS or the Lectin buffer. Also, if you are doing a non-IHC direct lectin-biotin staining, you must do the correct negative control. For SNA, this is an elution with 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid to finish elution. Buffer alone is NOT a negative control. For the lectins we worked with, we diluted the lectin (working concentration) in the recommended mM inhibition sugar and let it sit in the refrigerator overnight, warmed to RT just before use as negative control. This allows the lectin to bind to its specific sugar, and not to glycoproteins in the tissue, but keeps the biotin, and in our case, fluorophore in the negative control. There is an excellent, inexpensive book, Lectin Histochemistry, a Concise Practical Handbook by SA Brooks, AJC Leathem and U Schumacher that tells all about using many lectins, protocols for lectin IHC and lectin direct binding staining. An interesting side history is the founder of Vector is a lectin expert. For those doing IHC with anti-lectin, antigen retrieval may be needed per James Watson reply and is included in the Lectin IHC protocol I have. I am presuming he was doing true IHC for his lectin work. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Wednesday, April 16, 2014 10:53 AM To: M.O. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hi Merissa, don't know if you got any private idea responses so I'll throw in my opinion. I would always worry about some of the things you are mentioning and that are standard thoughts regarding biotin block, retrieval, etc in IHC. But I would think about your serum, which I steadfastly avoided with SNA or any lectin I used. Lectins look at glyco components and serum (or serum substitutes) can be full of glycoproteins and the target then is the blocking serum for your lectin which can cause bad background. I did and would use washes, diluents, etc that had NO serum or milk or anything like that in them. You can make your own, completely free of potentially having glycoproteins or Vector sells some. For some lectins (look at a list of target sugars) you maybe can get by with serum or milk and such to block but many I've found you just can't. Ray (still in, whoever would have guessed, once again rainy Seattle) ----- Original Message ----- From: "M.O." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 15, 2014 5:59:51 PM Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). I have never stained with anything like this, so I ran a test. I deparaffinized, blocked with NGS, incubated overnight at 4C with the diluted biotinylated SNA. On the second day, I used Vector's ABC kit and alkaline phosphatase (red) kit. Once stained, I noticed a lot of background. After looking into the blocking step, would a biotin/avidin blocking step be the correct step instead of a serum because I don't have a secondary? How do I know what needs to be blocked - biotin, avidin or both? Is there a way to do this without a kit and use solutions I may have in my lab? Thank you for your help! Sincerely, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Wed Apr 16 20:03:21 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Apr 16 20:03:29 2014 Subject: [Histonet] RE: Tracking control blocks In-Reply-To: <0111BC10D77DC54EAB99B2DDA3BCE4B96F5FA5@EXCH-2K10.hrhs.com> References: <0111BC10D77DC54EAB99B2DDA3BCE4B96F5FA5@EXCH-2K10.hrhs.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E022AB2C7@HHCEXCHMB03.hhcsystem.org> The vast majority of our positive control tissues are prepared from "left-over" tissue not used for patient diagnosis. I am hesitant to use the diagnostic tissue for control purposes in case we have to go back to perform additional testing. Also, I try to identify cases where we can put-through at least 20-30 tissue blocks. All control cases are entered into a "FileMaker Pro" file that I created years ago. Each case is given a "C" number and we enter the actual case number, the specimen site, the diagnosis, the time and date that the tissue is placed in formalin, the date that the tissue is processed, and immunoreactivity data (e.g., CK-MNF116+, HER2+, ALK1+, etc.). This program allows us to search any field using free text; very helpful when identifying a case for control purposes. All of our positive control slides have the "C" number on them. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, April 16, 2014 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking control blocks Happy Easter everyone!!! I was wondering if anyone had a tracking system that they would be willing to share in regards to how you all are keeping a record of your control blocks for IHC. As of right now, we are cutting a slide and staining it for the pathologist to approve- then keeping the paperwork and slide in files, and putting a label on the block in use with the date of approval. Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Michael.LaFriniere <@t> ccplab.com Thu Apr 17 07:48:58 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Thu Apr 17 07:52:53 2014 Subject: [Histonet] RE: Xylene testing ANP 08216 In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E058D8@SBS2K8.premierlab.local> References: <631955447A364B45B9458D2905635110AC107856@WIN08-MBX-01.wtbyhosp.org> <14E2C6176416974295479C64A11CB9AE019C79E058D8@SBS2K8.premierlab.local> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29669F24@AHCMSASEXCH02.my.ahc.local> All of my labs are tested at least yearly or when we change a major procedure dealing with formalin or xylene. In addition if an associate feels the fumes are high we test that day. When I CAP inspect I like to see the lab tests annually, however, it's not the requirement but would recommend as a caution to demonstrating a safe environment. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, April 15, 2014 11:41 AM To: Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Xylene testing ANP 08216 We test every year. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 15, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene testing ANP 08216 Hey Everyone, Just curious what people are doing with xylene testing? Are people really only doing the initial testing and never testing again if all was good? Seems like there should be periodic testing after decades have gone by! Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.LaFriniere <@t> ccplab.com Thu Apr 17 07:57:37 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Thu Apr 17 07:57:49 2014 Subject: [Histonet] IHC imaging systems In-Reply-To: References: Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29669F5D@AHCMSASEXCH02.my.ahc.local> I am setting up the Ventana system and performing validation, it's taking a little longer to set up then expected but the support has been excellent. Hopefully will have it running and validated in the next few months. Validation is the time consumer I found with setting up a system. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 15, 2014 12:59 PM To: HistoNet Subject: [Histonet] IHC imaging systems Hello out there in Histoland, If any of you out there in the clinical environment are using imaging systems to quantify IHC slides, please let me know what system you are using. We are currently using a system that was provided through Dako, but they no longer support it. So time to consider buying a new one. It needs to have an algorithm that allows the system to distinguish between labelled and not labelled cells and the percentage of each. Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Thu Apr 17 08:02:36 2014 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Apr 17 08:02:44 2014 Subject: [Histonet] IHC imaging systems In-Reply-To: <4A2A16B9707CE04E9CB6C82DC18C1D29669F5D@AHCMSASEXCH02.my.ahc.local> References: , <4A2A16B9707CE04E9CB6C82DC18C1D29669F5D@AHCMSASEXCH02.my.ahc.local> Message-ID: Amen... the time investment for validation! Celebrate when it is completed :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Michael.LaFriniere@ccplab.com > To: patpxs@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 17 Apr 2014 12:57:37 +0000 > Subject: RE: [Histonet] IHC imaging systems > CC: > > I am setting up the Ventana system and performing validation, it's taking a little longer to set up then expected but the support has been excellent. Hopefully will have it running and validated in the next few months. Validation is the time consumer I found with setting up a system. > > Michael R. LaFriniere, HT (ASCP) > Executive Director > > Capital Choice Pathology Laboratory > 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 > P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 > michael.lafriniere@CCPLab.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Tuesday, April 15, 2014 12:59 PM > To: HistoNet > Subject: [Histonet] IHC imaging systems > > Hello out there in Histoland, > > If any of you out there in the clinical environment are using imaging > systems to quantify IHC slides, please let me know what system you are > using. > > We are currently using a system that was provided through Dako, but they no > longer support it. So time to consider buying a new one. It needs to have > an algorithm that allows the system to distinguish between labelled and not > labelled cells and the percentage of each. > > Thanks in advance, > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.LaFriniere <@t> ccplab.com Thu Apr 17 08:12:19 2014 From: Michael.LaFriniere <@t> ccplab.com (Michael LaFriniere) Date: Thu Apr 17 08:12:30 2014 Subject: [Histonet] RE: Waste Flammable Info Please In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E058DC@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79E058DC@SBS2K8.premierlab.local> Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29669F96@AHCMSASEXCH02.my.ahc.local> I found each State that I have been in has a little different method to hazardous waste handling/reporting in the laboratory. I have found that recycling alcohol, xylene and formalin greatly reduces cost; my labs demonstrate 60-70% cost reduction in all areas of concern, (usage, disposal and regulation costs). However, you must invest time for a successful "program", that includes ample space, training and monitoring to assure its maximum benefit. In today's environment of decreasing laboratory reimbursement this is a major "LEAN" process that can be highly valuable in the pathology laboratory! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, April 15, 2014 1:21 PM To: Dennis Hahn; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Waste Flammable Info Please Dennis First of all we are in Colorado. If you dispose of more than 650 gallons a year in waste you are considered a small quantity generator, that amounts to one 55 gallon drum a month. Once you become a SQG this requires being registered with the EPA, additional safety training, every other year on site audits (In Colorado the Colorado Department of Public Health takes care of this) and then the other year a self-certification check list needs to be completed. If you dispose of less than 650 gallons you are considered a conditionally exempt small quantity generator and are not required to be registered with the EPA. Since you are disposing of 1 to 2 - 55 gallon drums a month that would mean that you would need to decrease your waste by at most 660 gallons a year or by 50%. When we recycled only alcohol in 2013 it looked like we still had around 1 - 55 gallon of waste per month. In 2014 We started recycling alcohol, xylene and proper, so far this year we have only had 2 - 55 gallons of waste picked up, that accounts for a 50% decrease in total waste, so it might be possible for you to start recycling and decrease your waste stream by 50%. We have also seen a cost saving in the purchasing of both alcohol and xylene the amount we purchase has decreased I don't have the specifics on the amount I just know we order less often that we used to. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, April 15, 2014 10:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Waste Flammable Info Please I have an inquiry specifically for Texas and/or Children's hospitals: What is your current volume of waste flammables and how do you handle it? Are you recycling, or is it removed by an outside vendor? A combination of the two? Suggestions on recycling instrumentation? Any drawbacks? We currently collect all waste flammable materials into a 55 Gallon drum and it is removed once or twice a month by a contracted vendor. The problem is, we are disposing of so much now that the vendor is requiring the medical center to obtain a high-use license involving the state and the EPA. We have evaluated recycling many years ago and found the process to be slow and sort of cumbersome. I know that the process has improved quite a bit over the years, but what about the tech time, tech exposure, time for recycling, etc.? Have you found it to be highly cost effective? Thanks in advance for any info you can share. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mmarti <@t> cmrb.eu Thu Apr 17 09:41:08 2014 From: mmarti <@t> cmrb.eu (Marti Gaudes, Merce) Date: Thu Apr 17 09:44:11 2014 Subject: [Histonet] human cells in rat tissue Message-ID: <3d47706b1b9f4e1898dc4d7bc68b799f@CMRBMAIL.cmrb.name> Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Merc? Mart? Gaudes Histology Unit ________________________________ P Abans d'imprimir aquest missatge, si us plau, comprova que ?s realment necessari. El medi ambient ?s cosa de tots. La informaci? continguda en aquest missatge i en qualsevol fitxer adjunt ?s confidencial, privada i d'?s exclusiu per al destinatari. Si no ?s la persona a la qual anava dirigida aquesta informaci?, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c?pia, divulgaci?, distribuci? o utilitzaci? noautoritzada d'aquest correu electr?nic i dels seus adjunts est? prohibida en virtut de la legislaci? vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La informaci?n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci?n, por favor, notifique inmediatamente el envio err?neo al remitente y borre el mensaje. Cualquier copia, divulgaci?n, distribuci?n o utilizaci?n no autorizada de este correo electr?nico y de sus adjuntos est? prohibida en virtud de la legislaci?n vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. From liz <@t> premierlab.com Thu Apr 17 09:53:06 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 17 09:53:11 2014 Subject: [Histonet] RE: human cells in rat tissue In-Reply-To: <3d47706b1b9f4e1898dc4d7bc68b799f@CMRBMAIL.cmrb.name> References: <3d47706b1b9f4e1898dc4d7bc68b799f@CMRBMAIL.cmrb.name> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05916@SBS2K8.premierlab.local> Merce There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall. We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study. We might try it again. We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce Sent: Thursday, April 17, 2014 8:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] human cells in rat tissue Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Merc? Mart? Gaudes Histology Unit ________________________________ P Abans d'imprimir aquest missatge, si us plau, comprova que ?s realment necessari. El medi ambient ?s cosa de tots. La informaci? continguda en aquest missatge i en qualsevol fitxer adjunt ?s confidencial, privada i d'?s exclusiu per al destinatari. Si no ?s la persona a la qual anava dirigida aquesta informaci?, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c?pia, divulgaci?, distribuci? o utilitzaci? noautoritzada d'aquest correu electr?nic i dels seus adjunts est? prohibida en virtut de la legislaci? vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La informaci?n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci?n, por favor, notifique inmediatamente el envio err?neo al remitente y borre el mensaje. Cualquier copia, divulgaci?n, distribuci?n o utilizaci?n no autorizada de este correo electr?nico y de sus adjuntos est? prohibida en virtud de la legislaci?n vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi <@t> nrh-ok.com Thu Apr 17 11:19:44 2014 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Thu Apr 17 11:19:49 2014 Subject: [Histonet] Billing Question Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B8220E3@MAIL.nrhnt.nrh-ok.com> Hi, Please I have a question on the new IHC Billing Policies for CPT, Medicare. My question is how do we go about the billing of the HER-2 DUAL ISH and the Kappa and Lambda ISH? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From Donna.Millard <@t> prlnet.com Thu Apr 17 12:21:45 2014 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Thu Apr 17 12:21:53 2014 Subject: [Histonet] RE: Billing Question Message-ID: <64D11CD058311340A1966C234C6B0B8930386E85@PRLEXCH02.prlnet.com> ISH is a different bill-code--88365. The changes were made to 88342. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From jstaruk <@t> masshistology.com Thu Apr 17 12:45:40 2014 From: jstaruk <@t> masshistology.com (Mass Histology) Date: Thu Apr 17 12:45:40 2014 Subject: [Histonet] RE: human cells in rat tissue In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E05916@SBS2K8.premierlab.local> References: <3d47706b1b9f4e1898dc4d7bc68b799f@CMRBMAIL.cmrb.name> <14E2C6176416974295479C64A11CB9AE019C79E05916@SBS2K8.premierlab.local> Message-ID: <05e201cf5a64$d9f73500$8de59f00$@masshistology.com> We use an anti-human mitochondria antibody that works well. Jim _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, April 17, 2014 10:53 AM To: Marti Gaudes, Merce; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: human cells in rat tissue Merce There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall. We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study. We might try it again. We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce Sent: Thursday, April 17, 2014 8:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] human cells in rat tissue Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Merc? Mart? Gaudes Histology Unit ________________________________ P Abans d'imprimir aquest missatge, si us plau, comprova que ?s realment necessari. El medi ambient ?s cosa de tots. La informaci? continguda en aquest missatge i en qualsevol fitxer adjunt ?s confidencial, privada i d'?s exclusiu per al destinatari. Si no ?s la persona a la qual anava dirigida aquesta informaci?, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c?pia, divulgaci?, distribuci? o utilitzaci? noautoritzada d'aquest correu electr?nic i dels seus adjunts est? prohibida en virtut de la legislaci? vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La informaci?n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci?n, por favor, notifique inmediatamente el envio err?neo al remitente y borre el mensaje. Cualquier copia, divulgaci?n, distribuci?n o utilizaci?n no autorizada de este correo electr?nico y de sus adjuntos est? prohibida en virtud de la legislaci?n vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7356 - Release Date: 04/17/14 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7331 - Release Date: 04/11/14 From liz <@t> premierlab.com Thu Apr 17 13:35:27 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 17 13:35:33 2014 Subject: [Histonet] RE: human cells in rat tissue In-Reply-To: <05e201cf5a64$d9f73500$8de59f00$@masshistology.com> References: <3d47706b1b9f4e1898dc4d7bc68b799f@CMRBMAIL.cmrb.name> <14E2C6176416974295479C64A11CB9AE019C79E05916@SBS2K8.premierlab.local> <05e201cf5a64$d9f73500$8de59f00$@masshistology.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0591D@SBS2K8.premierlab.local> We have used that too and it will work well in most instances, but there are some issues with that method, detection is entirely dependent upon the number of mitochondria in the cells and some cells contain more than others, as long you understand that you are fine. We feel we get a better staining reaction with the HLA-1 verses the mitochondria antibody. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Mass Histology [mailto:jstaruk@masshistology.com] Sent: Thursday, April 17, 2014 11:46 AM To: Elizabeth Chlipala; 'Marti Gaudes, Merce'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: human cells in rat tissue We use an anti-human mitochondria antibody that works well. Jim _______________________ James E.?Staruk HT(ASCP) ?www.masshistology.com ?? www.nehorselabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, April 17, 2014 10:53 AM To: Marti Gaudes, Merce; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: human cells in rat tissue Merce There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall. We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study. We might try it again. We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce Sent: Thursday, April 17, 2014 8:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] human cells in rat tissue Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Merc? Mart? Gaudes Histology Unit ________________________________ P Abans d'imprimir aquest missatge, si us plau, comprova que ?s realment necessari. El medi ambient ?s cosa de tots. La informaci? continguda en aquest missatge i en qualsevol fitxer adjunt ?s confidencial, privada i d'?s exclusiu per al destinatari. Si no ?s la persona a la qual anava dirigida aquesta informaci?, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c?pia, divulgaci?, distribuci? o utilitzaci? noautoritzada d'aquest correu electr?nic i dels seus adjunts est? prohibida en virtut de la legislaci? vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La informaci?n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci?n, por favor, notifique inmediatamente el envio err?neo al remitente y borre el mensaje. Cualquier copia, divulgaci?n, distribuci?n o utilizaci?n no autorizada de este correo electr?nico y de sus adjuntos est? prohibida en virtud de la legislaci?n vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7356 - Release Date: 04/17/14 ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7331 - Release Date: 04/11/14 From loreli173 <@t> mac.com Thu Apr 17 19:18:10 2014 From: loreli173 <@t> mac.com (Lori Gemeinhardt) Date: Thu Apr 17 19:18:19 2014 Subject: [Histonet] Xylene substitute for processing GI biopsies Message-ID: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori From dosiagwu <@t> unilag.edu.ng Thu Apr 17 22:52:16 2014 From: dosiagwu <@t> unilag.edu.ng (DAVID OSIAGWU) Date: Thu Apr 17 22:25:01 2014 Subject: [Histonet] Re: Histonet Digest, Vol 125, Issue 20 In-Reply-To: <20140417164921.6E82A9811F@mailgw.unilag.edu.ng> Message-ID: <491684735.5339084.1397793136753.JavaMail.root@unilag.edu.ng> Hi Gayle, i saw your response to Merissa, quite good. i have also been having problems with lectin IHC and will appreciate it if you can also forward me your Lectin IHC protocol, and your recipe for the Lectin buffer. Thank you Regards. Daniel Daniel Osiagwu, MS Dept. of Anatomic and Molecular Pathology College of Medicine. University of Lagos. +2348188225457 dosiagwu@unilag.edu.ng ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 17, 2014 5:49:21 PM Subject: Histonet Digest, Vol 125, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Staining FFPE with biotinylated SNA - how to block? (James Watson) 2. Tracking control blocks (Fawn Bomar) 3. RE: Staining FFPE with biotinylated SNA - how to block? (gayle callis) 4. RE: Tracking control blocks (Cartun, Richard) 5. RE: Xylene testing ANP 08216 (Michael LaFriniere) 6. RE: IHC imaging systems (Michael LaFriniere) 7. RE: IHC imaging systems (joelle weaver) 8. RE: Waste Flammable Info Please (Michael LaFriniere) 9. human cells in rat tissue (Marti Gaudes, Merce) 10. RE: human cells in rat tissue (Elizabeth Chlipala) 11. Billing Question (Adesupo, Adesuyi (Banjo)) ---------------------------------------------------------------------- Message: 1 Date: Wed, 16 Apr 2014 18:15:58 +0000 From: James Watson Subject: [Histonet] RE: Staining FFPE with biotinylated SNA - how to block? To: "koellingr@comcast.net" , M.O. Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="utf-8" With our lectin staining on paraffin embedded tissue we use Avidin/Biotin blocking prior to antibody application. Some protocols we do use HIER before the blocking. One important item is that some lectins require a specific diluent. The dilution can be as high as 1:12,000. Lectins are well known for binding with multiple tissue entities, sometimes you can reduce the staining on unwanted tissue entities by reducing your incubation time or dilution. We do not use serum blocking. For PNA we use: PNA Diluent Calcium Chloride?????????????????????..????????????????????? 0.0111 gm. Magnesium Chloride??????????????????????????????????????? 0.0203 gm. Manganese Chloride??????????????????????????????????????? 0.0125 gm. Distilled Water?????????????????????????????????????????????.???. 100.0 ml James Watson HT?? ASCP GNF?? Genomics Institute of the Novartis Research Foundation Tel?????? 858-332-4647 Fax???? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Wednesday, April 16, 2014 9:53 AM To: M.O. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hi Merissa, don't know if you got any private idea responses so I'll throw in my opinion.?? I would always worry about some of the things you are mentioning and that are standard thoughts regarding biotin block, retrieval, etc in IHC. But I would think about your serum, which I steadfastly avoided with SNA or any lectin I used.???? Lectins look at glyco components and serum (or serum substitutes) can be full of glycoproteins and the target then is the blocking serum for your lectin which can cause bad background.?? I did and would use washes, diluents, etc that had NO serum or milk or anything like that in them.?? You can make your own, completely free of potentially having??glycoproteins or Vector sells some.?? For some lectins (look at a list of target sugars) you maybe can get by with serum or milk and such to block??but many I've found you just can't. Ray (still in, whoever would have guessed, once again rainy Seattle) ----- Original Message ----- From: "M.O." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 15, 2014 5:59:51 PM Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. ??The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). ??I have never stained with anything like this, so I ran a test. I deparaffinized, blocked with NGS, incubated overnight at 4C with the diluted biotinylated SNA. ??On the second day, I used Vector's ABC kit and alkaline phosphatase (red) kit. Once stained, I noticed a lot of background. ??After looking into the blocking step, would a biotin/avidin blocking step be the correct step instead of a serum because I don't have a secondary? ??How do I know what needs to be blocked - biotin, avidin or both? ??Is there a way to do this without a kit and use solutions I may have in my lab? Thank you for your help! Sincerely, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 16 Apr 2014 18:30:00 +0000 From: Fawn Bomar Subject: [Histonet] Tracking control blocks To: "histonet@lists.utsouthwestern.edu" Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B96F5FA5@EXCH-2K10.hrhs.com> Content-Type: text/plain; charset="iso-8859-1" Happy Easter everyone!!! I was wondering if anyone had a tracking system that they would be willing to share in regards to how you all are keeping a record of your control blocks for IHC. As of right now, we are cutting a slide and staining it for the pathologist to approve- then keeping the paperwork and slide in files, and putting a label on the block in use with the date of approval. Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 3 Date: Wed, 16 Apr 2014 15:13:57 -0600 From: "gayle callis" Subject: RE: [Histonet] Staining FFPE with biotinylated SNA - how to block? To: "Histonet " Message-ID: <003801cf59b8$c9509630$5bf1c290$@bresnan.net> Content-Type: text/plain; charset="UTF-8" Dear Merissa, Ray is correct about using serums with lectins. In fact you really are NOT doing immunostaining unless you are using an antibody made to recognize that lectin e.g. anti-lectin therefore a normal serum block is not needed. Serums in fact contain glycoproteins that bind to lectins, so BSA is a better protein carrier for buffers and diluents if you want to make up your own. We used Jacksons immunoglobulin protease free BSA. Ray gave good advice on blocking techniques. However, if you do anti-Lectin, you would be doing IHC and then blocking is legal. If you want a protocol for lectin IHC, I will be happy to forward via private email. With some lectins for direct staining, you should use the Lectin buffer that has no phosphate ions, as explained by Vector technical services. I also have a recipe for the Lectin buffer if you want it. Avidin/biotin blocking is still needed with biotinylated lectin, particularly is the tissue is known to have endogenous biotin. Be sure to find out if your lectin is sensitive to phosphate ions, or use TBS or the Lectin buffer. Also, if you are doing a non-IHC direct lectin-biotin staining, you must do the correct negative control. For SNA, this is an elution with 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid to finish elution. Buffer alone is NOT a negative control. For the lectins we worked with, we diluted the lectin (working concentration) in the recommended mM inhibition sugar and let it sit in the refrigerator overnight, warmed to RT just before use as negative control. This allows the lectin to bind to its specific sugar, and not to glycoproteins in the tissue, but keeps the biotin, and in our case, fluorophore in the negative control. There is an excellent, inexpensive book, Lectin Histochemistry, a Concise Practical Handbook by SA Brooks, AJC Leathem and U Schumacher that tells all about using many lectins, protocols for lectin IHC and lectin direct binding staining. An interesting side history is the founder of Vector is a lectin expert. For those doing IHC with anti-lectin, antigen retrieval may be needed per James Watson reply and is included in the Lectin IHC protocol I have. I am presuming he was doing true IHC for his lectin work. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Wednesday, April 16, 2014 10:53 AM To: M.O. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hi Merissa, don't know if you got any private idea responses so I'll throw in my opinion. I would always worry about some of the things you are mentioning and that are standard thoughts regarding biotin block, retrieval, etc in IHC. But I would think about your serum, which I steadfastly avoided with SNA or any lectin I used. Lectins look at glyco components and serum (or serum substitutes) can be full of glycoproteins and the target then is the blocking serum for your lectin which can cause bad background. I did and would use washes, diluents, etc that had NO serum or milk or anything like that in them. You can make your own, completely free of potentially having glycoproteins or Vector sells some. For some lectins (look at a list of target sugars) you maybe can get by with serum or milk and such to block but many I've found you just can't. Ray (still in, whoever would have guessed, once again rainy Seattle) ----- Original Message ----- From: "M.O." To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 15, 2014 5:59:51 PM Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). I have never stained with anything like this, so I ran a test. I deparaffinized, blocked with NGS, incubated overnight at 4C with the diluted biotinylated SNA. On the second day, I used Vector's ABC kit and alkaline phosphatase (red) kit. Once stained, I noticed a lot of background. After looking into the blocking step, would a biotin/avidin blocking step be the correct step instead of a serum because I don't have a secondary? How do I know what needs to be blocked - biotin, avidin or both? Is there a way to do this without a kit and use solutions I may have in my lab? Thank you for your help! Sincerely, Merissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 17 Apr 2014 01:03:21 +0000 From: "Cartun, Richard" Subject: [Histonet] RE: Tracking control blocks To: Fawn Bomar , "histonet@lists.utsouthwestern.edu" Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E022AB2C7@HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" The vast majority of our positive control tissues are prepared from "left-over" tissue not used for patient diagnosis. I am hesitant to use the diagnostic tissue for control purposes in case we have to go back to perform additional testing. Also, I try to identify cases where we can put-through at least 20-30 tissue blocks. All control cases are entered into a "FileMaker Pro" file that I created years ago. Each case is given a "C" number and we enter the actual case number, the specimen site, the diagnosis, the time and date that the tissue is placed in formalin, the date that the tissue is processed, and immunoreactivity data (e.g., CK-MNF116+, HER2+, ALK1+, etc.). This program allows us to search any field using free text; very helpful when identifying a case for control purposes. All of our positive control slides have the "C" number on them. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Wednesday, April 16, 2014 2:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tracking control blocks Happy Easter everyone!!! I was wondering if anyone had a tracking system that they would be willing to share in regards to how you all are keeping a record of your control blocks for IHC. As of right now, we are cutting a slide and staining it for the pathologist to approve- then keeping the paperwork and slide in files, and putting a label on the block in use with the date of approval. Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 5 Date: Thu, 17 Apr 2014 12:48:58 +0000 From: Michael LaFriniere Subject: [Histonet] RE: Xylene testing ANP 08216 To: "histonet@lists.utsouthwestern.edu" Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29669F24@AHCMSASEXCH02.my.ahc.local> Content-Type: text/plain; charset="us-ascii" All of my labs are tested at least yearly or when we change a major procedure dealing with formalin or xylene. In addition if an associate feels the fumes are high we test that day. When I CAP inspect I like to see the lab tests annually, however, it's not the requirement but would recommend as a caution to demonstrating a safe environment. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, April 15, 2014 11:41 AM To: Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Xylene testing ANP 08216 We test every year. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 15, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene testing ANP 08216 Hey Everyone, Just curious what people are doing with xylene testing? Are people really only doing the initial testing and never testing again if all was good? Seems like there should be periodic testing after decades have gone by! Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 17 Apr 2014 12:57:37 +0000 From: Michael LaFriniere Subject: RE: [Histonet] IHC imaging systems To: Paula Sicurello , HistoNet Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29669F5D@AHCMSASEXCH02.my.ahc.local> Content-Type: text/plain; charset="us-ascii" I am setting up the Ventana system and performing validation, it's taking a little longer to set up then expected but the support has been excellent. Hopefully will have it running and validated in the next few months. Validation is the time consumer I found with setting up a system. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 15, 2014 12:59 PM To: HistoNet Subject: [Histonet] IHC imaging systems Hello out there in Histoland, If any of you out there in the clinical environment are using imaging systems to quantify IHC slides, please let me know what system you are using. We are currently using a system that was provided through Dako, but they no longer support it. So time to consider buying a new one. It needs to have an algorithm that allows the system to distinguish between labelled and not labelled cells and the percentage of each. Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 17 Apr 2014 13:02:36 +0000 From: joelle weaver Subject: RE: [Histonet] IHC imaging systems To: Michael LaFriniere , Paula Sicurello , HistoNet Message-ID: Content-Type: text/plain; charset="iso-8859-1" Amen... the time investment for validation! Celebrate when it is completed :) Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Michael.LaFriniere@ccplab.com > To: patpxs@gmail.com; histonet@lists.utsouthwestern.edu > Date: Thu, 17 Apr 2014 12:57:37 +0000 > Subject: RE: [Histonet] IHC imaging systems > CC: > > I am setting up the Ventana system and performing validation, it's taking a little longer to set up then expected but the support has been excellent. Hopefully will have it running and validated in the next few months. Validation is the time consumer I found with setting up a system. > > Michael R. LaFriniere, HT (ASCP) > Executive Director > > Capital Choice Pathology Laboratory > 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 > P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 > michael.lafriniere@CCPLab.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Tuesday, April 15, 2014 12:59 PM > To: HistoNet > Subject: [Histonet] IHC imaging systems > > Hello out there in Histoland, > > If any of you out there in the clinical environment are using imaging > systems to quantify IHC slides, please let me know what system you are > using. > > We are currently using a system that was provided through Dako, but they no > longer support it. So time to consider buying a new one. It needs to have > an algorithm that allows the system to distinguish between labelled and not > labelled cells and the percentage of each. > > Thanks in advance, > > Paula > > -- > Paula Sicurello, HTL (ASCP) > Supervisor, Clinical Electron Microscopy Laboratory > Duke University Health System > Rm.#251M, Duke South, Green Zone > Durham, North Carolina 27710 > P: 919.684.2091 > > HIPAA Privacy Notification: This message and any accompanying documents are > covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, > and contain information intended for the specific individual (s) only. This > information is confidential. If you are not the intended recipient or an > agent responsible for delivering it to the intended recipient, you are > hereby notified that you have received this document in error and that any > review, dissemination, copying or the taking of any action based on the > contents of this information is strictly prohibited . If you have received > this communication in error, please notify us immediately by e-mail, and > delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 17 Apr 2014 13:12:19 +0000 From: Michael LaFriniere Subject: [Histonet] RE: Waste Flammable Info Please To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <4A2A16B9707CE04E9CB6C82DC18C1D29669F96@AHCMSASEXCH02.my.ahc.local> Content-Type: text/plain; charset="us-ascii" I found each State that I have been in has a little different method to hazardous waste handling/reporting in the laboratory. I have found that recycling alcohol, xylene and formalin greatly reduces cost; my labs demonstrate 60-70% cost reduction in all areas of concern, (usage, disposal and regulation costs). However, you must invest time for a successful "program", that includes ample space, training and monitoring to assure its maximum benefit. In today's environment of decreasing laboratory reimbursement this is a major "LEAN" process that can be highly valuable in the pathology laboratory! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafriniere@CCPLab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, April 15, 2014 1:21 PM To: Dennis Hahn; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Waste Flammable Info Please Dennis First of all we are in Colorado. If you dispose of more than 650 gallons a year in waste you are considered a small quantity generator, that amounts to one 55 gallon drum a month. Once you become a SQG this requires being registered with the EPA, additional safety training, every other year on site audits (In Colorado the Colorado Department of Public Health takes care of this) and then the other year a self-certification check list needs to be completed. If you dispose of less than 650 gallons you are considered a conditionally exempt small quantity generator and are not required to be registered with the EPA. Since you are disposing of 1 to 2 - 55 gallon drums a month that would mean that you would need to decrease your waste by at most 660 gallons a year or by 50%. When we recycled only alcohol in 2013 it looked like we still had around 1 - 55 gallon of waste per month. In 2014 We started recycling alcohol, xylene and proper, so far this year we have only had 2 - 55 gallons of waste picked up, that accounts for a 50% decrease in total waste, so it might be possible for you to start recycling and decrease your waste stream by 50%. We have also seen a cost saving in the purchasing of both alcohol and xylene the amount we purchase has decreased I don't have the specifics on the amount I just know we order less often that we used to. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, April 15, 2014 10:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Waste Flammable Info Please I have an inquiry specifically for Texas and/or Children's hospitals: What is your current volume of waste flammables and how do you handle it? Are you recycling, or is it removed by an outside vendor? A combination of the two? Suggestions on recycling instrumentation? Any drawbacks? We currently collect all waste flammable materials into a 55 Gallon drum and it is removed once or twice a month by a contracted vendor. The problem is, we are disposing of so much now that the vendor is requiring the medical center to obtain a high-use license involving the state and the EPA. We have evaluated recycling many years ago and found the process to be slow and sort of cumbersome. I know that the process has improved quite a bit over the years, but what about the tech time, tech exposure, time for recycling, etc.? Have you found it to be highly cost effective? Thanks in advance for any info you can share. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 17 Apr 2014 14:41:08 +0000 From: "Marti Gaudes, Merce" Subject: [Histonet] human cells in rat tissue To: "Histonet@lists.utsouthwestern.edu" Message-ID: <3d47706b1b9f4e1898dc4d7bc68b799f@CMRBMAIL.cmrb.name> Content-Type: text/plain; charset="iso-8859-1" Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Merc? Mart? Gaudes Histology Unit ________________________________ P Abans d'imprimir aquest missatge, si us plau, comprova que ?s realment necessari. El medi ambient ?s cosa de tots. La informaci? continguda en aquest missatge i en qualsevol fitxer adjunt ?s confidencial, privada i d'?s exclusiu per al destinatari. Si no ?s la persona a la qual anava dirigida aquesta informaci?, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c?pia, divulgaci?, distribuci? o utilitzaci? noautoritzada d'aquest correu electr?nic i dels seus adjunts est? prohibida en virtut de la legislaci? vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La informaci?n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci?n, por favor, notifique inmediatamente el envio err?neo al remitente y borre el mensaje. Cualquier copia, divulgaci?n, distribuci?n o utilizaci?n no autorizada de este correo electr?nico y de sus adjuntos est? prohibida en virtud de la legislaci?n vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. ------------------------------ Message: 10 Date: Thu, 17 Apr 2014 08:53:06 -0600 From: Elizabeth Chlipala Subject: [Histonet] RE: human cells in rat tissue To: "Marti Gaudes, Merce" , "Histonet@lists.utsouthwestern.edu" Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05916@SBS2K8.premierlab.local> Content-Type: text/plain; charset="iso-8859-1" Merce There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall. We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study. We might try it again. We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce Sent: Thursday, April 17, 2014 8:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] human cells in rat tissue Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Merc? Mart? Gaudes Histology Unit ________________________________ P Abans d'imprimir aquest missatge, si us plau, comprova que ?s realment necessari. El medi ambient ?s cosa de tots. La informaci? continguda en aquest missatge i en qualsevol fitxer adjunt ?s confidencial, privada i d'?s exclusiu per al destinatari. Si no ?s la persona a la qual anava dirigida aquesta informaci?, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol c?pia, divulgaci?, distribuci? o utilitzaci? noautoritzada d'aquest correu electr?nic i dels seus adjunts est? prohibida en virtut de la legislaci? vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La informaci?n contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta informaci?n, por favor, notifique inmediatamente el envio err?neo al remitente y borre el mensaje. Cualquier copia, divulgaci?n, distribuci?n o utilizaci?n no autorizada de este correo electr?nico y de sus adjuntos est? prohibida en virtud de la legislaci?n vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 17 Apr 2014 11:19:44 -0500 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] Billing Question To: "histonet@lists.utsouthwestern.edu" Message-ID: <04EE4F75BB5FB246ADB68D69B74604438E3B8220E3@MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Hi, Please I have a question on the new IHC Billing Policies for CPT, Medicare. My question is how do we go about the billing of the HER-2 DUAL ISH and the Kappa and Lambda ISH? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 125, Issue 20 ***************************************** From liz <@t> premierlab.com Fri Apr 18 09:08:50 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Apr 18 09:08:56 2014 Subject: [Histonet] Awards Spotlight - Biocare Medical Excellence in Patient Care Laboratory Award Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0592A@SBS2K8.premierlab.local> Biocare Medical Excellence in Patient Care Laboratory Award Biocare Medical sponsors this award with a $1500 grant. This award is presented annually to a histology laboratory that exhibits excellence in patient care. Efficient testing turnaround time, error free reporting, willingness to incorporate new techniques, dedication of laboratory staff and extraordinary levels of laboratory performance are qualities that the recipient of this laboratory award exhibits. This grant is intended to: 1. Enhance laboratory efficiency through continuing education 2. Enhance laboratory techniques presently utilized in the laboratory 3. Support the study of new techniques under consideration for adoption in the nominated laboratory Some of the past recipients of this award are Diagnostic Services of Mantobia in 2013, William Beaumont Hospital in 2011 and Caris Life Sciences in 2009. Do you feel your lab meets the above criteria and should be recognized for increased patient care, then get your medical director or pathologist to nominate the lab for this award. http://www.nsh.org/scholarships-awards/education/laboratory%20awards Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From rjbuesa <@t> yahoo.com Fri Apr 18 10:14:52 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Apr 18 10:15:03 2014 Subject: [Histonet] Xylene substitute for processing GI biopsies In-Reply-To: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> References: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> Message-ID: <1397834092.31286.YahooMailNeo@web120405.mail.ne1.yahoo.com> Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can?use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html?and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J.? On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute.? I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience!? This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Apr 18 10:39:50 2014 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Apr 18 10:42:43 2014 Subject: [Histonet] Xylene substitute for processing GI biopsies In-Reply-To: <1397834092.31286.YahooMailNeo@web120405.mail.ne1.yahoo.com> References: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> <1397834092.31286.YahooMailNeo@web120405.mail.ne1.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E391681F4925C@IBMB7Exchange.digestivespecialists.com> Lori, We use Formula 83 from CBG for both processing and staining. We have used this for years with no problem for our GI biopsies. It wouldn't do any good to share my processing schedule with you though since we have a different processor. If you want to talk or any further information feel free to contact me. We also recycle our Formula 83. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can?use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html?and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J.? On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute.? I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience!? This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Apr 18 11:41:28 2014 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Apr 18 11:41:38 2014 Subject: [Histonet] Xylene substitute for processing GI biopsies In-Reply-To: <5A2BD13465E061429D6455C8D6B40E391681F4925C@IBMB7Exchange.digestivespecialists.com> References: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> <1397834092.31286.YahooMailNeo@web120405.mail.ne1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E391681F4925C@IBMB7Exchange.digestivespecialists.com> Message-ID: We have used Americlear for many, many years. Processing and staining of everything (not just GI). It's a d-limonene product so it does have an orangish smell. I have always had VIPs and we have never had a problem. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, April 18, 2014 11:40 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitute for processing GI biopsies Lori, We use Formula 83 from CBG for both processing and staining. We have used this for years with no problem for our GI biopsies. It wouldn't do any good to share my processing schedule with you though since we have a different processor. If you want to talk or any further information feel free to contact me. We also recycle our Formula 83. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From liz <@t> premierlab.com Fri Apr 18 11:46:24 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Apr 18 11:46:30 2014 Subject: [Histonet] Xylene substitute for processing GI biopsies In-Reply-To: References: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> <1397834092.31286.YahooMailNeo@web120405.mail.ne1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E391681F4925C@IBMB7Exchange.digestivespecialists.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0593B@SBS2K8.premierlab.local> We use propar on the tissue processor for mouse and rat eyes (you need three stations of proper) for adequate processing. Works great for us, we do not use it in staining but I know it will work for that also. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, April 18, 2014 10:41 AM To: 'Blazek, Linda'; Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitute for processing GI biopsies We have used Americlear for many, many years. Processing and staining of everything (not just GI). It's a d-limonene product so it does have an orangish smell. I have always had VIPs and we have never had a problem. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, April 18, 2014 11:40 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitute for processing GI biopsies Lori, We use Formula 83 from CBG for both processing and staining. We have used this for years with no problem for our GI biopsies. It wouldn't do any good to share my processing schedule with you though since we have a different processor. If you want to talk or any further information feel free to contact me. We also recycle our Formula 83. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From krns <@t> regionsjaelland.dk Fri Apr 18 13:26:42 2014 From: krns <@t> regionsjaelland.dk (Karen Inge Nielsen) Date: Fri Apr 18 13:26:49 2014 Subject: SV: [Histonet] Xylene substitute for processing GI biopsies In-Reply-To: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> References: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> Message-ID: <8A886B98D8F46141B553250B4B5A134366443E39@SVSLS103V223.regsj.intern> Hi Since you use a tissue tec VIP from Sakura you could use tissue clear from Sakura. I have been a histotech for 11 years an have only used xylene substitute for processin. The past 3 years on Peloris from Leica and before that on VIP E300. I use Tissue Clear from Sakura and it works as well as xylene. You just need to entlong the clearing steps a little. I use also Tissue Clear in the stainingprocess. Just go for it.... I work xylenefree with results as good as xyleneprotocols. Kind regards Karen ________________________________________ Fra: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] på vegne af Lori Gemeinhardt [loreli173@mac.com] Sendt: 18. april 2014 02:18 Til: histonet@lists.utsouthwestern.edu Emne: [Histonet] Xylene substitute for processing GI biopsies Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJohnson <@t> gnf.org Fri Apr 18 13:27:44 2014 From: TJohnson <@t> gnf.org (Teri Johnson) Date: Fri Apr 18 13:27:59 2014 Subject: : [Histonet] Xylene substitute for processing GI biopsies Message-ID: <9F3CFEE76E51B64991C7485270890B40498A2889@EX5.lj.gnf.org> Hi Lori, Pretty much any xylene sub will work for processing tissues and staining. You may need to add additional time in the "xylene" steps, since the action of the subs isn't as quick as xylene. In addition, the substitutes are more intolerant of water. How humid is your lab? If it's dry, then you may not have to make any additional adjustment. Otherwise you may need to make sure your final absolute alcohol is fairly anhydrous or water will collect in the bottom of your container, potentially causing problems with processing. Molecular sieves can be used to keep that from happening, or you can add additional containers of absolute alcohol (and decrease the time accordingly in each one) so there isn't as much water carryover by the time it reaches the first container of substitute. Some people are sensitive to the orange smell of the Limonene subs. I always liked it, but it gave headaches to my supervisor when we were trying it. Plus it is more oily than xylene so your slides might take longer to dry after coverslipping. If you coverslip out of xylene sub, you need to you have a mounting medium compatible with the substitute. Check with the manufacturer to see what they recommend. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 From histotex <@t> sbcglobal.net Sat Apr 19 10:52:59 2014 From: histotex <@t> sbcglobal.net (Sherry Martin) Date: Sat Apr 19 10:55:45 2014 Subject: [Histonet] Hiring Histologist In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E0593B@SBS2K8.premierlab.local> References: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> <1397834092.31286.YahooMailNeo@web120405.mail.ne1.yahoo.com> <5A2BD13465E061429D6455C8D6B40E391681F4925C@IBMB7Exchange.digestivespecialists.com> <14E2C6176416974295479C64A11CB9AE019C79E0593B@SBS2K8.premierlab.local> Message-ID: <1397922779.69085.YahooMailNeo@web185304.mail.gq1.yahoo.com> Small lab in Greenville, Texas is seeking a certified Histologist. E-mail for details. Thanks!? ? Sherry Martin, H.T. (ASCP) Histology Supervisor histotex@sbcglobal.net From histotalk <@t> yahoo.com Sat Apr 19 21:38:09 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Sat Apr 19 21:41:26 2014 Subject: [Histonet] HistoTALK - Wanda Simons & Shirley Powell Message-ID: <1397961489.27318.YahooMailNeo@web141102.mail.bf1.yahoo.com> Hello - Great to have Wanda and Shirley on the show! www.HistoTALK.com HISTOPALOOZA here we come! Check out the GSH website The Georgia Society of Histotechnology?for all the HISTOPOLOOZA HAPPENINGS! The Georgia Society of Histotechnology The Georgia Society for Histotechnology GSH OFFICERS: Wanda Simons??? President gshpresident@gmail.com Michael B... View on www.HistoSearch.com Preview by Yahoo Yours, Dave From joewalker <@t> rrmc.org Sun Apr 20 18:36:05 2014 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Sun Apr 20 18:36:12 2014 Subject: [Histonet] Xylene substitute for processing GI biopsies In-Reply-To: <1397834092.31286.YahooMailNeo@web120405.mail.ne1.yahoo.com> References: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> <1397834092.31286.YahooMailNeo@web120405.mail.ne1.yahoo.com> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC182A088D@RRMBX03.rrmc.local> Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub? We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From Ronald.Houston <@t> nationwidechildrens.org Mon Apr 21 08:12:12 2014 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Mon Apr 21 08:12:20 2014 Subject: [Histonet] Alport's testing Message-ID: Does anyone test for Alport's (alpha 1, 3 & 5 chains of collagen IV) on paraffin sections? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From rjbuesa <@t> yahoo.com Mon Apr 21 08:56:23 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Apr 21 08:59:23 2014 Subject: [Histonet] Xylene substitute for processing GI biopsies In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC182A088D@RRMBX03.rrmc.local> References: <701FF845-15E2-47DA-A4C7-E430C70FE6EC@mac.com> <1397834092.31286.YahooMailNeo@web120405.mail.ne1.yahoo.com> <3C2378778400AD448ADA6FD6BDB7CCCC182A088D@RRMBX03.rrmc.local> Message-ID: <1398088583.10371.YahooMailNeo@web120405.mail.ne1.yahoo.com> Theoretically YES! Ren? J. On Sunday, April 20, 2014 7:36 PM, "Joe W. Walker, Jr." wrote: Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub? We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790? F: 802.747.6525 Email joewalker@rrmc.org? ? www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute.? I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience!? This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From histo <@t> skm.org.pk Mon Apr 21 09:01:36 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Mon Apr 21 09:00:15 2014 Subject: [Histonet] Recommendations cryostat Message-ID: Hi All, We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. Regards Muhammad Tahseen SKMCH&RC From ecameron1 <@t> midcoasthealth.com Mon Apr 21 09:15:29 2014 From: ecameron1 <@t> midcoasthealth.com (Cameron, Elizabeth) Date: Mon Apr 21 09:15:35 2014 Subject: [Histonet] RE: Paraffin In-Reply-To: <7EAFE982E328304DA6CE2B677BB762468719B64B@TN001WEXMBX12.US.chs.net> References: , <7EAFE982E328304DA6CE2B677BB762468719B64B@TN001WEXMBX12.US.chs.net> Message-ID: I am still trying to figure out what I need to do for validation for CAP. Do I need to revalidate IHC and/or special stains if I change the paraffin on the processor? The paraffin I have in mind is supposed to work faster than the one we currently use for infiltration, so we may need to adjust times. Thanks. ________________________________________ From: Boyd, Debbie M [DKBoyd@chs.net] Sent: Wednesday, April 16, 2014 10:17 AM To: Cameron, Elizabeth; histonet@lists.utsouthwestern.edu Subject: RE: Paraffin We use Richard Allen Type 6 paraffin for both infiltration and embedding (16 years) with no adverse effects. To validate you can get the pathologist to give you samples for the same specimens you are running. IE; gallbladder 1 for patient dx and 1 for testing, uterus, appendix, any large specimen that you can spare an extra slice. Run the patient dx specimens one night, change only the paraffin in your processor and embedding center run the test samples during the day while the processor isn't in use for patient specimens. Cut and stain per usual as time permits. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cameron, Elizabeth Sent: Wednesday, April 16, 2014 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Hello histonetters, I recently made the move back to a hospital lab after being in research, and we are currently using two different parrafins for infiltration and embedding. I would like to change the type of paraffin that we are using (I have one in mind), but I was wondering if there were any advantages to using different paraffins for the different steps and what type of validation would need to be done to make this change. We only have one processor, so a side by side comparison would be very difficult. Any thoughts on the matter would be greatly appreciated. Thank you, Liz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jpiche <@t> wtbyhosp.org Mon Apr 21 09:26:17 2014 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Mon Apr 21 09:26:29 2014 Subject: [Histonet] COM 30450 Message-ID: <631955447A364B45B9458D2905635110AC107C84@WIN08-MBX-01.wtbyhosp.org> Hello Everyone, Does COM 30450 apply to Diff Quik kits and reagents used in special stains (other stain kits)? Or does the lot to lot testing only apply to things such as IHC? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From wdesalvo.cac <@t> outlook.com Mon Apr 21 09:28:46 2014 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon Apr 21 09:28:50 2014 Subject: [Histonet] Recommendations cryostat In-Reply-To: References: Message-ID: Best recommendation I can provide is the Leica 1950 was the hands down choice of the employees performing frozen sections at multiple hospital sites in my system. From my perspective, the instruments are very reliable, easy to use, easy to maintain and pathologists approve of the the slide quality. William DeSalvo, BS HTL(ASCP) > From: histo@skm.org.pk > To: histonet@lists.utsouthwestern.edu > Date: Mon, 21 Apr 2014 14:01:36 +0000 > CC: tahseen0009@gmail.com > Subject: [Histonet] Recommendations cryostat > > Hi All, > We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. > Regards > Muhammad Tahseen > SKMCH&RC > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra <@t> isisph.com Mon Apr 21 09:35:33 2014 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Mon Apr 21 09:35:59 2014 Subject: [Histonet] RE: Recommendations cryostat In-Reply-To: References: Message-ID: Leica! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor Sent: Monday, April 21, 2014 7:02 AM To: histonet@lists.utsouthwestern.edu Cc: tahseen0009@gmail.com Subject: [Histonet] Recommendations cryostat Hi All, We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. Regards Muhammad Tahseen SKMCH&RC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anna.c.hughes <@t> gsk.com Mon Apr 21 09:55:13 2014 From: anna.c.hughes <@t> gsk.com (Anna Hughes) Date: Mon Apr 21 09:56:00 2014 Subject: [Histonet] anti mouse iNOS Message-ID: <56B8D7633148A9419B559EBE8E9CF5900E2DCD8A@019-SN1MPN4-151.019D.MGD.MSFT.NET> Hi Everyone! Anyone have a good antibody for an anti mouse iNOS antibody that they especially like? Thanks! Anna Anna C. Hughes anna.c.hughes@gsk.com From kim.tournear <@t> yahoo.com Mon Apr 21 11:12:58 2014 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Mon Apr 21 11:13:06 2014 Subject: [Histonet] RE: Recommendations cryostat In-Reply-To: References: Message-ID: Definitely Leica Sent from the iPhone of Kim Tournear ?? ? On Apr 21, 2014, at 7:35 AM, Bea DeBrosse-Serra wrote: > Leica! > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor > Sent: Monday, April 21, 2014 7:02 AM > To: histonet@lists.utsouthwestern.edu > Cc: tahseen0009@gmail.com > Subject: [Histonet] Recommendations cryostat > > Hi All, > We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. > Regards > Muhammad Tahseen > SKMCH&RC > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Millard <@t> prlnet.com Mon Apr 21 12:14:34 2014 From: Donna.Millard <@t> prlnet.com (Donna Millard) Date: Mon Apr 21 12:14:44 2014 Subject: [Histonet] RE: Xylene substitute for processing GI biopsies Message-ID: <64D11CD058311340A1966C234C6B0B8930387B7C@PRLEXCH02.prlnet.com> Joe, Yes. We are about to go xylene-free (though we are still planning on using if for cleaning, because the feedback we got was that we still needed to clean the processors with xylene or we would run into problems, and my supervisor heard from people that they had to go back to xylene flushing if they initially stopped). We are going with an odorless substitute because the orange-smelling ones bother some people in the lab. We did a full validation (with blinded evaluations) that included special stains and IHC with all retrieval methods, and Breast Imaging. CAP validation requirements are getting stricter, and new IHC validation guidelenes just came out from CAP, so I would recommend validating. FYI-routinely our pathologists selected the slides with substitute processing over xylene processed (though it was close). Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 From Karen.Kay <@t> albertahealthservices.ca Mon Apr 21 12:27:10 2014 From: Karen.Kay <@t> albertahealthservices.ca (Karen Kay) Date: Mon Apr 21 12:27:16 2014 Subject: [Histonet] CRYOSTAT RECOMMENDATIONSRE: Histonet Digest, Vol 125, Issue 24 In-Reply-To: <20140421170155.5625E310030@scutcheon.calgaryhealthregion.ca> References: <20140421170155.5625E310030@scutcheon.calgaryhealthregion.ca> Message-ID: Good ;Morning, We have the Leica 1950 in our Laboratory and are extremely pleased with the insturment. Karen J Kay, MLT Supervisor - Histopathology and Cytology Laboratory Chinook Regional Hospital - South Zone West - Alberta Health Services Lethbridge, Alberta, Canada karen.kay@albertahealthservices.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: April 21, 2014 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 125, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Xylene substitute for processing GI biopsies (Joe W. Walker, Jr.) 2. Alport's testing (Houston, Ronald) 3. Re: Xylene substitute for processing GI biopsies (Rene J Buesa) 4. Recommendations cryostat (Pathology-Histology Sr. Supervisor) 5. RE: Paraffin (Cameron, Elizabeth) 6. COM 30450 (Piche, Jessica) 7. RE: Recommendations cryostat (WILLIAM DESALVO) 8. RE: Recommendations cryostat (Bea DeBrosse-Serra) 9. anti mouse iNOS (Anna Hughes) 10. Re: RE: Recommendations cryostat (Kim Tournear) ---------------------------------------------------------------------- Message: 1 Date: Sun, 20 Apr 2014 23:36:05 +0000 From: "Joe W. Walker, Jr." Subject: RE: [Histonet] Xylene substitute for processing GI biopsies To: Rene J Buesa , Lori Gemeinhardt , "histonet@lists.utsouthwestern.edu" Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC182A088D@RRMBX03.rrmc.local> Content-Type: text/plain; charset="iso-8859-1" Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub? We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ------------------------------ Message: 2 Date: Mon, 21 Apr 2014 13:12:12 +0000 From: "Houston, Ronald" Subject: [Histonet] Alport's testing To: Histonet , "ihcrg@googlegroups.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone test for Alport's (alpha 1, 3 & 5 chains of collagen IV) on paraffin sections? Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster ------------------------------ Message: 3 Date: Mon, 21 Apr 2014 06:56:23 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Xylene substitute for processing GI biopsies To: "Joe W. Walker, Jr." , Lori Gemeinhardt , "histonet@lists.utsouthwestern.edu" Message-ID: <1398088583.10371.YahooMailNeo@web120405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Theoretically YES! Ren? J. On Sunday, April 20, 2014 7:36 PM, "Joe W. Walker, Jr." wrote: Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub? We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790? F: 802.747.6525 Email joewalker@rrmc.org? ? www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, April 18, 2014 11:15 AM To: Lori Gemeinhardt; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene substitute for processing GI biopsies Hi Lori: Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping. If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols. If you follow the instructions, you will have a totally xylene free lab. Ren? J. On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt wrote: Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute.? I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience!? This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ------------------------------ Message: 4 Date: Mon, 21 Apr 2014 14:01:36 +0000 From: "Pathology-Histology Sr. Supervisor" Subject: [Histonet] Recommendations cryostat To: "histonet@lists.utsouthwestern.edu" Cc: "tahseen0009@gmail.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi All, We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. Regards Muhammad Tahseen SKMCH&RC ------------------------------ Message: 5 Date: Mon, 21 Apr 2014 14:15:29 +0000 From: "Cameron, Elizabeth" Subject: [Histonet] RE: Paraffin To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am still trying to figure out what I need to do for validation for CAP. Do I need to revalidate IHC and/or special stains if I change the paraffin on the processor? The paraffin I have in mind is supposed to work faster than the one we currently use for infiltration, so we may need to adjust times. Thanks. ________________________________________ From: Boyd, Debbie M [DKBoyd@chs.net] Sent: Wednesday, April 16, 2014 10:17 AM To: Cameron, Elizabeth; histonet@lists.utsouthwestern.edu Subject: RE: Paraffin We use Richard Allen Type 6 paraffin for both infiltration and embedding (16 years) with no adverse effects. To validate you can get the pathologist to give you samples for the same specimens you are running. IE; gallbladder 1 for patient dx and 1 for testing, uterus, appendix, any large specimen that you can spare an extra slice. Run the patient dx specimens one night, change only the paraffin in your processor and embedding center run the test samples during the day while the processor isn't in use for patient specimens. Cut and stain per usual as time permits. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cameron, Elizabeth Sent: Wednesday, April 16, 2014 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Hello histonetters, I recently made the move back to a hospital lab after being in research, and we are currently using two different parrafins for infiltration and embedding. I would like to change the type of paraffin that we are using (I have one in mind), but I was wondering if there were any advantages to using different paraffins for the different steps and what type of validation would need to be done to make this change. We only have one processor, so a side by side comparison would be very difficult. Any thoughts on the matter would be greatly appreciated. Thank you, Liz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 6 Date: Mon, 21 Apr 2014 14:26:17 +0000 From: "Piche, Jessica" Subject: [Histonet] COM 30450 To: "histonet@lists.utsouthwestern.edu" Message-ID: <631955447A364B45B9458D2905635110AC107C84@WIN08-MBX-01.wtbyhosp.org> Content-Type: text/plain; charset="us-ascii" Hello Everyone, Does COM 30450 apply to Diff Quik kits and reagents used in special stains (other stain kits)? Or does the lot to lot testing only apply to things such as IHC? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ------------------------------ Message: 7 Date: Mon, 21 Apr 2014 07:28:46 -0700 From: WILLIAM DESALVO Subject: RE: [Histonet] Recommendations cryostat To: "Pathology-Histology Sr. Supervisor" , histonet Cc: "tahseen0009@gmail.com" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Best recommendation I can provide is the Leica 1950 was the hands down choice of the employees performing frozen sections at multiple hospital sites in my system. From my perspective, the instruments are very reliable, easy to use, easy to maintain and pathologists approve of the the slide quality. William DeSalvo, BS HTL(ASCP) > From: histo@skm.org.pk > To: histonet@lists.utsouthwestern.edu > Date: Mon, 21 Apr 2014 14:01:36 +0000 > CC: tahseen0009@gmail.com > Subject: [Histonet] Recommendations cryostat > > Hi All, > We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. > Regards > Muhammad Tahseen > SKMCH&RC > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 21 Apr 2014 14:35:33 +0000 From: Bea DeBrosse-Serra Subject: [Histonet] RE: Recommendations cryostat To: "'Pathology-Histology Sr. Supervisor'" , "histonet@lists.utsouthwestern.edu" Cc: "tahseen0009@gmail.com" Message-ID: Content-Type: text/plain; charset="us-ascii" Leica! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor Sent: Monday, April 21, 2014 7:02 AM To: histonet@lists.utsouthwestern.edu Cc: tahseen0009@gmail.com Subject: [Histonet] Recommendations cryostat Hi All, We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. Regards Muhammad Tahseen SKMCH&RC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 21 Apr 2014 14:55:13 +0000 From: Anna Hughes Subject: [Histonet] anti mouse iNOS To: "histonet@lists.utsouthwestern.edu" Message-ID: <56B8D7633148A9419B559EBE8E9CF5900E2DCD8A@019-SN1MPN4-151.019D.MGD.MSFT.NET> Content-Type: text/plain; charset="us-ascii" Hi Everyone! Anyone have a good antibody for an anti mouse iNOS antibody that they especially like? Thanks! Anna Anna C. Hughes anna.c.hughes@gsk.com ------------------------------ Message: 10 Date: Mon, 21 Apr 2014 09:12:58 -0700 From: Kim Tournear Subject: Re: [Histonet] RE: Recommendations cryostat To: Bea DeBrosse-Serra Cc: "histonet@lists.utsouthwestern.edu" , "tahseen0009@gmail.com" Message-ID: Content-Type: text/plain; charset=utf-8 Definitely Leica Sent from the iPhone of Kim Tournear ???????? ???? On Apr 21, 2014, at 7:35 AM, Bea DeBrosse-Serra wrote: > Leica! > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Pathology-Histology Sr. Supervisor > Sent: Monday, April 21, 2014 7:02 AM > To: histonet@lists.utsouthwestern.edu > Cc: tahseen0009@gmail.com > Subject: [Histonet] Recommendations cryostat > > Hi All, > We are going to buy cryostat so opinion required CM 1950 LEICA verses HM525 NX Thermo. > Regards > Muhammad Tahseen > SKMCH&RC > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 125, Issue 24 ***************************************** This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. From CDuffett <@t> Pathlinelabs.com Mon Apr 21 13:34:35 2014 From: CDuffett <@t> Pathlinelabs.com (Chris Duffett) Date: Mon Apr 21 13:35:14 2014 Subject: [Histonet] Semen Analysis Training Message-ID: <88A5A9E3A858EC4D877EE80B993921B91158B00D6A@exchange> Does anyone know of a facility in the North Jersey or New York City area that has a semen analysis training program. We are looking for motility and morphology. We are familiar with the Cleveland Clinic program but it is a distance as well as expensive. Any input or suggestions would be helpful. Chris D ________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From TanyaAbbott <@t> catholichealth.net Tue Apr 22 06:40:10 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Tue Apr 22 06:40:16 2014 Subject: [Histonet] New Microwave Message-ID: <852F7D2C14FB464D80E182B15DB138AF30675250@CHIEX005.CHI.catholichealth.net> We have finally taken the plunge (thanks to our CAP inspection!) to a new beautiful stainless steel vented microwave to replace our kitchen one from the 80s! Any suggestions on how to meet all the CAP guidelines for this? We only use the microwave for Special Stains, not for processing. Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From liz <@t> premierlab.com Tue Apr 22 10:13:26 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 22 10:13:38 2014 Subject: [Histonet] Awards Spotlight - JB McCormick, MD Award Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05951@SBS2K8.premierlab.local> Awards Spotlight - J. B. McCormick, MD Award Sponsored by: James and Suzanne McCormick Family Trust This award is generously sponsored by Dr. McCormick and his family. The award consists of a functioning exact replica of an 1800 Liberkuhn Compass Microscope and an eight book boxed set of The Science Heritage Library, concerning the history of the microscope and Histotechnology. Award Criteria: This award is presented for outstanding and exceptional service to the National Society for Histotechnology. The McCormick Family Trust has been sponsoring this award since 1978, that's pretty amazing. Thanks Dr. McCormick! Some of the past recipients are Freida Carson in 1983, Sheron Lear in 2001, Jan Minshew in 2008 and Skip Brown in 2011. You can see a full list of the recipients on the NSH website. If you know of anyone who has supported the NSH through the years and support can consist of many things, from lecturing at state and local meetings, to serving on a committee or as an elected official. These individuals are highly dedicated to their profession, if you know of someone that is deserving of this prestigious award then nominate them. http://nsh.org/scholarships-awards/advocacy/service%20awards?nid=1401 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From Vickroy.Jim <@t> mhsil.com Tue Apr 22 10:16:59 2014 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Apr 22 10:17:07 2014 Subject: [Histonet] Looking for TAu and Synuclein IVD antibodies Message-ID: We are using two antibodies that are RUO's. They are Tau and Synuclein. I have been searching for an IVD antibody instead. I noticed that DAKO has Tau in an IVD but I can't find an IVD for Synuclein. Anybody have any ideas for synuclein and comments on the DAKO Tau? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From santij1 <@t> bellsouth.net Tue Apr 22 10:46:39 2014 From: santij1 <@t> bellsouth.net (JERRY SANTIAGO) Date: Tue Apr 22 10:46:44 2014 Subject: [Histonet] Florida Society for Histotechnology Message-ID: <1398181599.67862.YahooMailNeo@web180904.mail.ne1.yahoo.com> Hello everyone, Four weeks left until the magic begins. The deadline for room reservations is tomorrow April 23rd. We are almost soldout for the weekend of May 15-18. Buena vista Hotal and Spa, Lake Buena Vista (Orlando) Florida. Reserve today! From hans <@t> histologistics.com Tue Apr 22 11:30:39 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Tue Apr 22 11:30:47 2014 Subject: [Histonet] Need very good used microscope Message-ID: <72DDB3F6-6A57-469C-8C28-DF4F052092C3@histologistics.com> We are looking for a used microscope with very good optics that can take a camera. Would like to spend no more that $2000.00. Does anyone have suggestions? Thank you Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com From gloria <@t> integrityhealthplus.com Tue Apr 22 15:54:58 2014 From: gloria <@t> integrityhealthplus.com (gloria@integrityhealthplus.com) Date: Tue Apr 22 15:55:06 2014 Subject: [Histonet] Need a Laboratory/Medical Assistant Message-ID: <006601cf5e6d$1ff01e30$5fd05a90$@integrityhealthplus.com> Hello Histology land, I have an opening for a full time position Monday-Friday for a Laboratory Assistant/Medical Assistant. This person will help out approximately three days a week in our laboratory in Farmers Branch (close to Addison), Tx. and two days a week at a doctor's office for specimen collection (one in town office). They must have reliable transportation. If you know of anyone interested in this position or finding out more information about this position please email me, Gloria, at Gloria@integrityhealthplus.com . Thank you From plott <@t> uab.edu Tue Apr 22 15:58:16 2014 From: plott <@t> uab.edu (Patricia F Lott) Date: Tue Apr 22 15:58:21 2014 Subject: [Histonet] need Linda Jenkins Message-ID: <372C4AF089B6AE48B36F064FA891FF7813896328@UABEXMB3.ad.uab.edu> Someone please send me the contact info for Linda Jenkins, formerly of Clemson University. From hrfulklab <@t> gmail.com Tue Apr 22 17:07:28 2014 From: hrfulklab <@t> gmail.com (H R) Date: Tue Apr 22 17:07:36 2014 Subject: [Histonet] Freelance Histology Message-ID: Has anyone heard of people cutting and staining slides out of your garage or some kind of similar set up, like picking up overflow from different companies /labs? I overheard a conversation about someone doing this in their retirement. Labs send them the block and all they do is cut and do HE stain and return both and bill? What kinds of regulations would that entail? I don't know if that could be true. Maybe for research facilities? -- *H* From liz <@t> premierlab.com Tue Apr 22 17:23:26 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 22 17:23:30 2014 Subject: [Histonet] Freelance Histology In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05971@SBS2K8.premierlab.local> Cutting is one thing such as like control slides, but staining that?s a whole different thing, you will have chemical waste that needs to be disposed of properly. You will need appropriate ventilation, etc. Correct me if I am wrong but I don?t think you could be performing any clinical work, it would have to be research. Even though you might be only one person I still think you are responsible for all applicable OSHA guidelines. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R Sent: Tuesday, April 22, 2014 4:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freelance Histology Has anyone heard of people cutting and staining slides out of your garage or some kind of similar set up, like picking up overflow from different companies /labs? I overheard a conversation about someone doing this in their retirement. Labs send them the block and all they do is cut and do HE stain and return both and bill? What kinds of regulations would that entail? I don't know if that could be true. Maybe for research facilities? -- *H* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Apr 22 17:28:09 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Apr 22 17:28:14 2014 Subject: [Histonet] Freelance Histology In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E05971@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79E05971@SBS2K8.premierlab.local> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05973@SBS2K8.premierlab.local> Please excuse my grammar, yikes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, April 22, 2014 4:23 PM To: H R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Freelance Histology Cutting is one thing such as like control slides, but staining that?s a whole different thing, you will have chemical waste that needs to be disposed of properly. You will need appropriate ventilation, etc. Correct me if I am wrong but I don?t think you could be performing any clinical work, it would have to be research. Even though you might be only one person I still think you are responsible for all applicable OSHA guidelines. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R Sent: Tuesday, April 22, 2014 4:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freelance Histology Has anyone heard of people cutting and staining slides out of your garage or some kind of similar set up, like picking up overflow from different companies /labs? I overheard a conversation about someone doing this in their retirement. Labs send them the block and all they do is cut and do HE stain and return both and bill? What kinds of regulations would that entail? I don't know if that could be true. Maybe for research facilities? -- *H* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Apr 22 17:43:04 2014 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Apr 22 17:57:40 2014 Subject: [Histonet] California Society for Histotechnology Message-ID: Don't miss out on some great work shops. The deadline for registration has been extended to this Friday and if you still need a room that can be arranged. For registration: http://californiahistology.org/events.html For rooms: contact Hilton reservations coordinator Yvette Moradians at 818-596-4506 From pruegg <@t> ihctech.net Tue Apr 22 19:25:40 2014 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Apr 22 19:25:45 2014 Subject: [Histonet] Freelance Histology In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79E05973@SBS2K8.premierlab.local> References: , <14E2C6176416974295479C64A11CB9AE019C79E05971@SBS2K8.premierlab.local>, <14E2C6176416974295479C64A11CB9AE019C79E05973@SBS2K8.premierlab.local> Message-ID: this may certainly not be on the up and up but I have heard about these kinds of operations for years, not sure how they can do it but I know of a lab that did clinical skin bx samples for years and years and I am sure they were not Clia/CAP/or even GLP, I guess since it is Histology all the responsibility lies with the Pathologist reading the slides, I never thought it was very kosher, at least for this one case it is not going on anymore, but it would not surprise me if there were Derm and GI labs all over not really accredited to be doing histology on human samples but they still do. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com pruegg@ihctech.net From: liz@premierlab.com To: liz@premierlab.com; hrfulklab@gmail.com; histonet@lists.utsouthwestern.edu Date: Tue, 22 Apr 2014 16:28:09 -0600 Subject: RE: [Histonet] Freelance Histology CC: Please excuse my grammar, yikes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, April 22, 2014 4:23 PM To: H R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Freelance Histology Cutting is one thing such as like control slides, but staining that?s a whole different thing, you will have chemical waste that needs to be disposed of properly. You will need appropriate ventilation, etc. Correct me if I am wrong but I don?t think you could be performing any clinical work, it would have to be research. Even though you might be only one person I still think you are responsible for all applicable OSHA guidelines. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R Sent: Tuesday, April 22, 2014 4:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Freelance Histology Has anyone heard of people cutting and staining slides out of your garage or some kind of similar set up, like picking up overflow from different companies /labs? I overheard a conversation about someone doing this in their retirement. Labs send them the block and all they do is cut and do HE stain and return both and bill? What kinds of regulations would that entail? I don't know if that could be true. Maybe for research facilities? -- *H* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotalk <@t> yahoo.com Wed Apr 23 06:57:48 2014 From: histotalk <@t> yahoo.com (David Kemler) Date: Wed Apr 23 06:57:52 2014 Subject: [Histonet] GSH Histopalooza Message-ID: <1398254268.35978.YahooMailNeo@web141101.mail.bf1.yahoo.com> Hi folks - The HistoTALK mobile unit is planning on arriving in Pine Mtn, GA for the GSH's 1st HISTOPALOOZA late tomorrow afternoon. Interviewing will begin Friday morning at 9 am. Remember, anyone can be a guest on the show, not only workshop presenters! Everyone is invited to talk about any histology related subject. Just stop by the 'mobile' unit (banquet table and three chairs). Look for the big bright yellow banner and let me know that you want to be a guest on the show and 'll have you scheduled. It's that easy.? It is very important that you understand that all interviews are taped for a latter broadcast. HistoTALK is not LIVE! Umms, ooo's, and aaah's are edited out prior to airing. HistoTALK is YOUR histology program - be part of it. Yours, Dave From rjbuesa <@t> yahoo.com Wed Apr 23 09:00:58 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 23 09:04:00 2014 Subject: [Histonet] Need very good used microscope In-Reply-To: <72DDB3F6-6A57-469C-8C28-DF4F052092C3@histologistics.com> References: <72DDB3F6-6A57-469C-8C28-DF4F052092C3@histologistics.com> Message-ID: <1398261658.79972.YahooMailNeo@web120405.mail.ne1.yahoo.com> Please log into eBay, they have just what you are looking for within your budget. Ren? J. On Tuesday, April 22, 2014 12:31 PM, Hans B Snyder wrote: We are looking for a used microscope? with very good optics that can take a camera.? Would like to spend no more that $2000.00.? Does anyone have suggestions? Thank you Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bruce_Palmatier <@t> vwr.com Wed Apr 23 09:57:20 2014 From: Bruce_Palmatier <@t> vwr.com (Bruce_Palmatier@vwr.com) Date: Wed Apr 23 09:57:30 2014 Subject: [Histonet] AUTO: Bruce Palmatier is out of the office (returning 04/25/2014) Message-ID: I am out of the office until 04/25/2014. I will be out of the office from April 23rd through April 25th. I will be checking emails periodically and will attempt to reply to your message within 24 hours. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmatier@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: HEALTHCARESERVICE@VWR.COM Note: This is an automated response to your message "Histonet Digest, Vol 125, Issue 25" sent on 4/23/2014 10:52:17 AM. This is the only notification you will receive while this person is away. From hans <@t> histologistics.com Wed Apr 23 12:06:41 2014 From: hans <@t> histologistics.com (Hans B Snyder) Date: Wed Apr 23 12:06:47 2014 Subject: [Histonet] Freelance Histology In-Reply-To: References: Message-ID: Several years ago while in the beginning stages of setting up my histology lab, I looked into this possibility (cutting at home). The town hall laughed and said it is illegal for a business to run out of ones house that uses chemicals and paraffin because of it's flammable and explosive properties. This made sense, but I also had heard stories of beginning companies who have done this. Therefore a business running out of a residential house is doing so illegally and maybe without insurance. Just my two cents. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Tue, Apr 22, 2014 at 6:07 PM, H R wrote: > Has anyone heard of people cutting and staining slides out of your garage > or some kind of similar set up, like picking up overflow from different > companies /labs? I overheard a conversation about someone doing this in > their retirement. Labs send them the block and all they do is cut and do HE > stain and return both and bill? What kinds of regulations would that > entail? I don't know if that could be true. Maybe for research facilities? > > -- > *H* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smglaboratory <@t> gmail.com Wed Apr 23 13:19:49 2014 From: smglaboratory <@t> gmail.com (April Disney) Date: Wed Apr 23 13:19:56 2014 Subject: [Histonet] Re: Histonet Digest, Vol 125, Issue 26 In-Reply-To: <5357f1bb.e599b60a.1485.ffff9c30SMTPIN_ADDED_MISSING@mx.google.com> References: <5357f1bb.e599b60a.1485.ffff9c30SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: RE: Freelance histology This kind of thing is not legal. Look up the "Stark Law." On Wed, Apr 23, 2014 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Need a Laboratory/Medical Assistant > (gloria@integrityhealthplus.com) > 2. need Linda Jenkins (Patricia F Lott) > 3. Freelance Histology (H R) > 4. RE: Freelance Histology (Elizabeth Chlipala) > 5. RE: Freelance Histology (Elizabeth Chlipala) > 6. California Society for Histotechnology (Jennifer MacDonald) > 7. RE: Freelance Histology (Patsy Ruegg) > 8. GSH Histopalooza (David Kemler) > 9. Re: Need very good used microscope (Rene J Buesa) > 10. AUTO: Bruce Palmatier is out of the office (returning > 04/25/2014) (Bruce_Palmatier@vwr.com) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 22 Apr 2014 15:54:58 -0500 > From: > Subject: [Histonet] Need a Laboratory/Medical Assistant > To: > Message-ID: <006601cf5e6d$1ff01e30$5fd05a90$@integrityhealthplus.com> > Content-Type: text/plain; charset="us-ascii" > > Hello Histology land, > > > > I have an opening for a full time position Monday-Friday for a Laboratory > Assistant/Medical Assistant. This person will help out approximately three > days a week in our laboratory in Farmers Branch (close to Addison), Tx. and > two days a week at a doctor's office for specimen collection (one in town > office). They must have reliable transportation. If you know of anyone > interested in this position or finding out more information about this > position please email me, Gloria, at Gloria@integrityhealthplus.com . > Thank > you > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 22 Apr 2014 20:58:16 +0000 > From: Patricia F Lott > Subject: [Histonet] need Linda Jenkins > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <372C4AF089B6AE48B36F064FA891FF7813896328@UABEXMB3.ad.uab.edu> > Content-Type: text/plain; charset="us-ascii" > > Someone please send me the contact info for Linda Jenkins, formerly of > Clemson University. > > > ------------------------------ > > Message: 3 > Date: Tue, 22 Apr 2014 18:07:28 -0400 > From: H R > Subject: [Histonet] Freelance Histology > To: histonet@lists.utsouthwestern.edu > Message-ID: > 0vkVTwe8yg@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Has anyone heard of people cutting and staining slides out of your garage > or some kind of similar set up, like picking up overflow from different > companies /labs? I overheard a conversation about someone doing this in > their retirement. Labs send them the block and all they do is cut and do HE > stain and return both and bill? What kinds of regulations would that > entail? I don't know if that could be true. Maybe for research facilities? > > -- > *H* > > > ------------------------------ > > Message: 4 > Date: Tue, 22 Apr 2014 16:23:26 -0600 > From: Elizabeth Chlipala > Subject: RE: [Histonet] Freelance Histology > To: H R , "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <14E2C6176416974295479C64A11CB9AE019C79E05971@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="utf-8" > > Cutting is one thing such as like control slides, but staining that???s a > whole different thing, you will have chemical waste that needs to be > disposed of properly. You will need appropriate ventilation, etc. > Correct me if I am wrong but I don???t think you could be performing any > clinical work, it would have to be research. Even though you might be only > one person I still think you are responsible for all applicable OSHA > guidelines. > > Just my two cents. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R > Sent: Tuesday, April 22, 2014 4:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Freelance Histology > > Has anyone heard of people cutting and staining slides out of your garage > or some kind of similar set up, like picking up overflow from different > companies /labs? I overheard a conversation about someone doing this in > their retirement. Labs send them the block and all they do is cut and do HE > stain and return both and bill? What kinds of regulations would that > entail? I don't know if that could be true. Maybe for research facilities? > > -- > *H* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 5 > Date: Tue, 22 Apr 2014 16:28:09 -0600 > From: Elizabeth Chlipala > Subject: RE: [Histonet] Freelance Histology > To: Elizabeth Chlipala , H R > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <14E2C6176416974295479C64A11CB9AE019C79E05973@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="utf-8" > > Please excuse my grammar, yikes. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala > Sent: Tuesday, April 22, 2014 4:23 PM > To: H R; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Freelance Histology > > Cutting is one thing such as like control slides, but staining that???s a > whole different thing, you will have chemical waste that needs to be > disposed of properly. You will need appropriate ventilation, etc. > Correct me if I am wrong but I don???t think you could be performing any > clinical work, it would have to be research. Even though you might be only > one person I still think you are responsible for all applicable OSHA > guidelines. > > Just my two cents. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box > 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R > Sent: Tuesday, April 22, 2014 4:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Freelance Histology > > Has anyone heard of people cutting and staining slides out of your garage > or some kind of similar set up, like picking up overflow from different > companies /labs? I overheard a conversation about someone doing this in > their retirement. Labs send them the block and all they do is cut and do HE > stain and return both and bill? What kinds of regulations would that > entail? I don't know if that could be true. Maybe for research facilities? > > -- > *H* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 6 > Date: Tue, 22 Apr 2014 15:43:04 -0700 > From: Jennifer MacDonald > Subject: [Histonet] California Society for Histotechnology > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > OFC789EC65.3D8780F0-ON88257CC2.007C7DDA-88257CC2.007CCA4F@mtsac.edu> > Content-Type: text/plain; charset="US-ASCII" > > Don't miss out on some great work shops. The deadline for registration > has been extended to this Friday and if you still need a room that can be > arranged. > > For registration: > http://californiahistology.org/events.html > > For rooms: > contact Hilton reservations coordinator Yvette Moradians at > 818-596-4506 > > ------------------------------ > > Message: 7 > Date: Tue, 22 Apr 2014 18:25:40 -0600 > From: Patsy Ruegg > Subject: RE: [Histonet] Freelance Histology > To: "liz@premierlab.com" , H R > , "Histonet@Lists. Edu" > > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > this may certainly not be on the up and up but I have heard about these > kinds of operations for years, not sure how they can do it but I know of a > lab that did clinical skin bx samples for years and years and I am sure > they were not Clia/CAP/or even GLP, I guess since it is Histology all the > responsibility lies with the Pathologist reading the slides, I never > thought it was very kosher, at least for this one case it is not going on > anymore, but it would not surprise me if there were Derm and GI labs all > over not really accredited to be doing histology on human samples but they > still do. > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > pruegg@ihctech.net > > > From: liz@premierlab.com > To: liz@premierlab.com; hrfulklab@gmail.com; > histonet@lists.utsouthwestern.edu > Date: Tue, 22 Apr 2014 16:28:09 -0600 > Subject: RE: [Histonet] Freelance Histology > CC: > > Please excuse my grammar, yikes. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala > Sent: Tuesday, April 22, 2014 4:23 PM > To: H R; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Freelance Histology > > Cutting is one thing such as like control slides, but staining that?s a > whole different thing, you will have chemical waste that needs to be > disposed of properly. You will need appropriate ventilation, etc. > Correct me if I am wrong but I don?t think you could be performing any > clinical work, it would have to be research. Even though you might be only > one person I still think you are responsible for all applicable OSHA > guidelines. > > Just my two cents. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box > 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R > Sent: Tuesday, April 22, 2014 4:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Freelance Histology > > Has anyone heard of people cutting and staining slides out of your garage > or some kind of similar set up, like picking up overflow from different > companies /labs? I overheard a conversation about someone doing this in > their retirement. Labs send them the block and all they do is cut and do HE > stain and return both and bill? What kinds of regulations would that > entail? I don't know if that could be true. Maybe for research facilities? > > -- > *H* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 8 > Date: Wed, 23 Apr 2014 04:57:48 -0700 (PDT) > From: David Kemler > Subject: [Histonet] GSH Histopalooza > To: Fellow HistoNetters > Message-ID: > <1398254268.35978.YahooMailNeo@web141101.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi folks - The HistoTALK mobile unit is planning on arriving in Pine Mtn, > GA for the GSH's 1st HISTOPALOOZA late tomorrow afternoon. > > Interviewing will begin Friday morning at 9 am. Remember, anyone can be a > guest on the show, not only workshop presenters! Everyone is invited to > talk about any histology related subject. > > Just stop by the 'mobile' unit (banquet table and three chairs). Look for > the big bright yellow banner and let me know that you want to be a guest on > the show and 'll have you scheduled. It's that easy. > > It is very important that you understand that all interviews are taped for > a latter broadcast. HistoTALK is not LIVE! Umms, ooo's, and aaah's are > edited out prior to airing. > > HistoTALK is YOUR histology program - be part of it. > > Yours, > Dave > > ------------------------------ > > Message: 9 > Date: Wed, 23 Apr 2014 07:00:58 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Need very good used microscope > To: Hans B Snyder , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <1398261658.79972.YahooMailNeo@web120405.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Please log into eBay, they have just what you are looking for within your > budget. > Ren? J. > On Tuesday, April 22, 2014 12:31 PM, Hans B Snyder < > hans@histologistics.com> wrote: > > We are looking for a used microscope with very good optics that can take > a camera. Would like to spend no more that $2000.00. Does anyone have > suggestions? > > Thank you > > Histologistics > Hans B Snyder > 508.308.7800 > Hans@histologistics.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 10 > Date: Wed, 23 Apr 2014 10:57:20 -0400 > From: Bruce_Palmatier@vwr.com > Subject: [Histonet] AUTO: Bruce Palmatier is out of the office > (returning 04/25/2014) > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > > I am out of the office until 04/25/2014. > > I will be out of the office from April 23rd through April 25th. I will be > checking emails periodically and will attempt to reply to your message > within 24 hours. > > Thank You, > > Bruce Palmatier > Market Portfolio Manager > VWR Healthcare > bruce_palmatier@vwr.com > mobile: 484.319.5563 > fax: 484-881-7307 > > Customer Service: 877.881.1192 > Fax: 484.881.6486 > Customer Service email: HEALTHCARESERVICE@VWR.COM > > > Note: This is an automated response to your message "Histonet Digest, Vol > 125, Issue 25" sent on 4/23/2014 10:52:17 AM. > > This is the only notification you will receive while this person is away. > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 125, Issue 26 > ***************************************** > From tkngflght <@t> yahoo.com Wed Apr 23 14:38:40 2014 From: tkngflght <@t> yahoo.com (Cheryl Kerry) Date: Wed Apr 23 14:39:24 2014 Subject: [Histonet] NBF without methanol ? Message-ID: Help? We've recently discovered our formalin has sufficient methanol to require special storage. What supplier do you use and does your formalin have little to no methanol -- with a flash point higher then 200 degrees? Please email me directly-- vendors welcome to respond. We use 1gallon and 5 gallon sized containers (83 and 1 a month respectively) Thank you-- Cheryl From mhanna <@t> histosearch.com Wed Apr 23 14:50:27 2014 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Wed Apr 23 14:50:34 2014 Subject: [Histonet] Posting Images for Histonet Message-ID: <53581983.3050808@histosearch.com> Hi Histonetters, Tim Morken asked me about posting images for discussion on Histonet a couple of weeks ago and we noticed our images website is no longer running. As we move into digital pathology, having the ability to post images to discuss on Histonet will become more important. So, we set up an easy way to post images for Histonet on Histosearch at http://histosearch.com/imageupload/. You need to fill in your name, a title for your images, select images to upload from your computer or smartphone and click submit. There is no need to register and it takes about 2 minutes. We intentionally did not include comments on the website so the discussions will take place on Histonet. We have also set up mobile pages for searching Histosearch and the archives at http://m.histosearch.com and http://m.histosearch.com/histonet.html for histologists on the go. You may also want to try out the new image searching capabilities on Histosearch . After searching with keyword(s) such as ki67 or alcian blue, there is a link at the top of the results for "images". Clicking this will provide images related to your keyword(s) from the over 12 million histopathology related pages searched in Histosearch. We believe we have the largest list of histology educational resources on the internet at Histosearch and they are now searchable for text and images. Let us know if we've missed any histology resources. Best Regards, Marvin Hanna From amber.mckenzie <@t> gastrodocs.net Wed Apr 23 14:55:02 2014 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Apr 23 14:55:06 2014 Subject: [Histonet] Oven/thermometer In-Reply-To: <4AD6A4E531E8C943A730559B6B81DF07CE9EBE@dc.Advancederm.net> References: <20140212172546.7BB421E805A@trendmess-svr.holyredeemer.local> <5A33C952BB67F4468AF1F36D739212BC0112463868@JERRY.Gia.com> <4AD6A4E531E8C943A730559B6B81DF07CE9EBE@dc.Advancederm.net> Message-ID: <5A33C952BB67F4468AF1F36D739212BC01124857D3@JERRY.Gia.com> Does anyone have any recommendations on a small slide oven to sit by the microtome to put immunos/specials in before staining? I have one I bought from Cardinal maybe 2 years ago and it's acting up. And the thermometer that sticks out the top is acting crazy as well. Thanks! From hrfulklab <@t> gmail.com Wed Apr 23 17:48:57 2014 From: hrfulklab <@t> gmail.com (H R) Date: Wed Apr 23 17:49:01 2014 Subject: [Histonet] Freelance Histology In-Reply-To: References: Message-ID: I don't think I would want to process or stain, but I think just cutting unstained sections would be safe. Maybe for research projects or what not. On Wed, Apr 23, 2014 at 1:06 PM, Hans B Snyder wrote: > Several years ago while in the beginning stages of setting up my > histology lab, I looked into this possibility (cutting at home). The > town hall laughed and said it is illegal for a business to run out of > ones house that uses chemicals and paraffin because of it's flammable > and explosive properties. This made sense, but I also had heard > stories of beginning companies who have done this. Therefore a > business running out of a residential house is doing so illegally and > maybe without insurance. > > Just my two cents. > Hans B Snyder > Histologistics > 60 Prescott Street > Worcester, MA 01605 > 508-308-7800 > hans@histologistics.com > > > On Tue, Apr 22, 2014 at 6:07 PM, H R wrote: >> Has anyone heard of people cutting and staining slides out of your garage >> or some kind of similar set up, like picking up overflow from different >> companies /labs? I overheard a conversation about someone doing this in >> their retirement. Labs send them the block and all they do is cut and do HE >> stain and return both and bill? What kinds of regulations would that >> entail? I don't know if that could be true. Maybe for research facilities? >> >> -- >> *H* >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ADuddey <@t> firsthealth.org Thu Apr 24 06:28:51 2014 From: ADuddey <@t> firsthealth.org (Duddey, Aimee) Date: Thu Apr 24 06:29:03 2014 Subject: [Histonet] I need opinions on Leica instruments Message-ID: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. Thank you in advance. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FirstHealth Moore Regional Hospital Pinehurst, NC 28374 (910) 715-5286 From JCBRITTON <@t> Cheshire-Med.COM Thu Apr 24 08:11:25 2014 From: JCBRITTON <@t> Cheshire-Med.COM (Britton, Josette C) Date: Thu Apr 24 08:11:35 2014 Subject: [Histonet] I need opinions on Leica instruments In-Reply-To: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> Message-ID: I love all of their instruments! Josie Britton, HT (ASCP) QIHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee Sent: Thursday, April 24, 2014 7:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I need opinions on Leica instruments I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. Thank you in advance. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FirstHealth Moore Regional Hospital Pinehurst, NC 28374 (910) 715-5286 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From kim.tournear <@t> yahoo.com Thu Apr 24 08:46:51 2014 From: kim.tournear <@t> yahoo.com (Kim Tournear) Date: Thu Apr 24 08:46:57 2014 Subject: [Histonet] I need opinions on Leica instruments In-Reply-To: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> References: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> Message-ID: Leica!!!!! Sent from the iPhone of Kim Tournear ?? ? On Apr 24, 2014, at 4:28 AM, "Duddey, Aimee" wrote: > I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. > Thank you in advance. > > Aimee M. Duddey, MLT(ASCP) > Assistant Director of Laboratory - Pathology > FirstHealth Moore Regional Hospital > Pinehurst, NC 28374 > (910) 715-5286 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DShank <@t> chpnet.org Thu Apr 24 09:00:05 2014 From: DShank <@t> chpnet.org (Deborah Shank) Date: Thu Apr 24 09:00:00 2014 Subject: [Histonet] RE: I need opinions on Leica instruments In-Reply-To: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> References: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> Message-ID: BOND III is excellent, I have 6. Deborah Shank, Manager Mt.Sinai Beth Israel Ny,NY Immunopathology, Flow Cytometry, Immunohistochemistry, FISH Tel 212 420 4049 FAX 212 420 4087 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee Sent: Thursday, April 24, 2014 7:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I need opinions on Leica instruments I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. Thank you in advance. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FirstHealth Moore Regional Hospital Pinehurst, NC 28374 (910) 715-5286 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attachments are confidential and intended solely for the use of the individual or entity to which they are addressed. If you are not the intended recipient, you are prohibited from printing, copying, forwarding, saving, or otherwise using or relying upon them in any manner. Please notify the sender immediately if you have received this message by mistake and delete it from your system. From michaela.tourville <@t> duke.edu Thu Apr 24 09:12:56 2014 From: michaela.tourville <@t> duke.edu (Michaela Lefaivre) Date: Thu Apr 24 09:13:37 2014 Subject: [Histonet] RE: I need opinions on Leica instruments In-Reply-To: References: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org>, Message-ID: We have the Bond III and love them! Michaela LeFaivre BS, HTL (ASCP) CM Molecular Technician IV Molecular Pathology Rm 4344 Purple Zone 919-684-4303 919-684-5095 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Deborah Shank [DShank@chpnet.org] Sent: Thursday, April 24, 2014 10:00 AM To: Duddey, Aimee; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: I need opinions on Leica instruments BOND III is excellent, I have 6. Deborah Shank, Manager Mt.Sinai Beth Israel Ny,NY Immunopathology, Flow Cytometry, Immunohistochemistry, FISH Tel 212 420 4049 FAX 212 420 4087 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee Sent: Thursday, April 24, 2014 7:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I need opinions on Leica instruments I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. Thank you in advance. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FirstHealth Moore Regional Hospital Pinehurst, NC 28374 (910) 715-5286 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attachments are confidential and intended solely for the use of the individual or entity to which they are addressed. If you are not the intended recipient, you are prohibited from printing, copying, forwarding, saving, or otherwise using or relying upon them in any manner. Please notify the sender immediately if you have received this message by mistake and delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.tibbs <@t> accuratediagnosticlabs.com Thu Apr 24 10:05:14 2014 From: barbara.tibbs <@t> accuratediagnosticlabs.com (Barbara Tibbs) Date: Thu Apr 24 10:05:24 2014 Subject: [Histonet] RE: I need opinions on Leica instruments In-Reply-To: References: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org>, , Message-ID: My lab is entirely Leica. Wouldn't have it any other way! Sent from my iPhone > On Apr 24, 2014, at 10:19 AM, "Michaela Lefaivre" wrote: > > We have the Bond III and love them! > > Michaela LeFaivre BS, HTL (ASCP) CM > > Molecular Technician IV > > Molecular Pathology > > Rm 4344 Purple Zone > > 919-684-4303 > > 919-684-5095 > > HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Deborah Shank [DShank@chpnet.org] > Sent: Thursday, April 24, 2014 10:00 AM > To: Duddey, Aimee; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: I need opinions on Leica instruments > > BOND III is excellent, I have 6. > > Deborah Shank, Manager > Mt.Sinai Beth Israel > Ny,NY > Immunopathology, Flow Cytometry, > Immunohistochemistry, FISH > Tel 212 420 4049 > FAX 212 420 4087 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee > Sent: Thursday, April 24, 2014 7:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] I need opinions on Leica instruments > > I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. > Thank you in advance. > > Aimee M. Duddey, MLT(ASCP) > Assistant Director of Laboratory - Pathology > FirstHealth Moore Regional Hospital > Pinehurst, NC 28374 > (910) 715-5286 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message and any attachments are confidential and intended solely > for the use of the individual or entity to which they are addressed. If > you are not the intended recipient, you are prohibited from printing, > copying, forwarding, saving, or otherwise using or relying upon them in > any manner. Please notify the sender immediately if you have received > this message by mistake and delete it from your system. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHKlei <@t> bellin.org Thu Apr 24 10:26:30 2014 From: SHKlei <@t> bellin.org (Shannon Kleiner) Date: Thu Apr 24 10:26:35 2014 Subject: [Histonet] I need opinions on Leica instruments In-Reply-To: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> References: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> Message-ID: <5358E6D6020000810000801F@mail.bellin.org> We have a Bond III and Bond Max, both very reliable and easy to use...No bad experience to report with either in the past two years. >>> "Duddey, Aimee" 4/24/2014 6:28 AM >>> I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. Thank you in advance. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FirstHealth Moore Regional Hospital Pinehurst, NC 28374 (910) 715-5286 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Apr 24 10:47:09 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 24 10:47:15 2014 Subject: [Histonet] Awards Spotlight - Tips to Writing an Excellent Letter of Recommendation Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05984@SBS2K8.premierlab.local> Awards Spotlight - Tips to Writing an Excellent Letter of Recommendation Today I'm not focusing on a specific award but providing some tips for those nominating others for awards. The Awards Committee has to sift through multiple nominations in order to choose the appropriate person for a particular award. We receive the persons CV and particulars but what really makes one individual stand out from the rest? I can tell you what that is - it's their letters of recommendations, so here are a few tips: 1. Get focused - understand what award you are nominating them for and focus in on the award criteria listed on the NSH website. 2. Get personal - what has that person accomplished? Don't generalize, we don't just want to know that they are a great histotech, we know that, that's why they have been nominated we want to hear WHAT makes them a great histotech, tell us what they have done, give us some examples, what makes this person stand out amongst others, the more personal the better. 3. Get multiple people to write letters - everyone has different experiences and different viewpoints. 4. And last of all - Get busy and nominate!!! The deadline for nominations is June 1st. http://nsh.org/scholarships-awards Thanks again Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From robinsoc <@t> mercyhealth.com Thu Apr 24 11:01:56 2014 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Apr 24 11:02:03 2014 Subject: [Histonet] I need opinions on Leica instruments In-Reply-To: <5358E6D6020000810000801F@mail.bellin.org> References: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> <5358E6D6020000810000801F@mail.bellin.org> Message-ID: <4EE642D353925D4D96CB95E12427DBAE5CB2B3@NODCMSTMBX06.no.trinity-health.org> We have the Peloris II and VIP processors, as well as Ventana.We are happy with the Peloris. It processes fatty tissue very well. The versatility with 2 retorts in one machine has allowed us to have several different processing times based on tissue size. We did have a learning curve with the Peloris as we have a smaller volume, <200 blocks per day. Initially we had to be more on top of the reagents and learn when to change versus waiting for the machine to signal a change. I wish Leica would have been a bit more helpful and up front about this. Leica did tell us that might have some issues due to our use of mesh cassettes and volume. I would be happy to answer questions if you would like to contact me directly. Cindi Robinson, HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Drive Dakota Dunes SD 57049 >>> "Duddey, Aimee" 4/24/2014 6:28 AM >>> I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. Thank you in advance. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FirstHealth Moore Regional Hospital Pinehurst, NC 28374 (910) 715-5286 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vtobias <@t> uw.edu Thu Apr 24 12:50:30 2014 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Thu Apr 24 12:52:18 2014 Subject: [Histonet] Pathology Report Templates Message-ID: One of our facilities had a CAP inspection last week and I was asked to add the CLIA# and Medical Director to the template, immediately. Is this something new, as we had our CAP inspection last Aug. and there was no mention about CLIA# or having the Director's name on the templates? I haven't been able to get any follow-up from the lab supervisor. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From JEllin <@t> yumaregional.org Thu Apr 24 12:54:22 2014 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Apr 24 12:54:49 2014 Subject: [Histonet] RE: Pathology Report Templates In-Reply-To: References: Message-ID: This is a fact, to meet compliance the signing facility needs to be ID along with the CLIA#, ,haven't heard about the medical director issue, but who knows -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Thursday, April 24, 2014 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology Report Templates One of our facilities had a CAP inspection last week and I was asked to add the CLIA# and Medical Director to the template, immediately. Is this something new, as we had our CAP inspection last Aug. and there was no mention about CLIA# or having the Director's name on the templates? I haven't been able to get any follow-up from the lab supervisor. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From john <@t> imebinc.com Thu Apr 24 12:57:33 2014 From: john <@t> imebinc.com (John O'Brien) Date: Thu Apr 24 12:57:44 2014 Subject: [Histonet] RE: Histonet Digest, Vol 125, Issue 27 Message-ID: <000d01cf5fe6$ab5c1850$021448f0$@imebinc.com> Hi Histonet- Can some offer me Jan Minshew formally with Leica new email address or phone # , I would like to send her a note. John Obrien IMEB INC CEO/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 125, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Freelance Histology (Hans B Snyder) 2. Re: Histonet Digest, Vol 125, Issue 26 (April Disney) 3. NBF without methanol ? (Cheryl Kerry) 4. Posting Images for Histonet (Marvin Hanna) 5. Oven/thermometer (Amber McKenzie) 6. Re: Freelance Histology (H R) 7. I need opinions on Leica instruments (Duddey, Aimee) 8. RE: I need opinions on Leica instruments (Britton, Josette C) 9. Re: I need opinions on Leica instruments (Kim Tournear) 10. RE: I need opinions on Leica instruments (Deborah Shank) 11. RE: I need opinions on Leica instruments (Michaela Lefaivre) 12. Re: RE: I need opinions on Leica instruments (Barbara Tibbs) 13. Re: I need opinions on Leica instruments (Shannon Kleiner) 14. Awards Spotlight - Tips to Writing an Excellent Letter of Recommendation (Elizabeth Chlipala) 15. RE: I need opinions on Leica instruments (Cynthia Robinson) ---------------------------------------------------------------------- Message: 1 Date: Wed, 23 Apr 2014 13:06:41 -0400 From: Hans B Snyder Subject: Re: [Histonet] Freelance Histology To: H R Cc: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Several years ago while in the beginning stages of setting up my histology lab, I looked into this possibility (cutting at home). The town hall laughed and said it is illegal for a business to run out of ones house that uses chemicals and paraffin because of it's flammable and explosive properties. This made sense, but I also had heard stories of beginning companies who have done this. Therefore a business running out of a residential house is doing so illegally and maybe without insurance. Just my two cents. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Tue, Apr 22, 2014 at 6:07 PM, H R wrote: > Has anyone heard of people cutting and staining slides out of your > garage or some kind of similar set up, like picking up overflow from > different companies /labs? I overheard a conversation about someone > doing this in their retirement. Labs send them the block and all they > do is cut and do HE stain and return both and bill? What kinds of > regulations would that entail? I don't know if that could be true. Maybe for research facilities? > > -- > *H* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 23 Apr 2014 11:19:49 -0700 From: April Disney Subject: [Histonet] Re: Histonet Digest, Vol 125, Issue 26 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=UTF-8 RE: Freelance histology This kind of thing is not legal. Look up the "Stark Law." On Wed, Apr 23, 2014 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Need a Laboratory/Medical Assistant > (gloria@integrityhealthplus.com) > 2. need Linda Jenkins (Patricia F Lott) > 3. Freelance Histology (H R) > 4. RE: Freelance Histology (Elizabeth Chlipala) > 5. RE: Freelance Histology (Elizabeth Chlipala) > 6. California Society for Histotechnology (Jennifer MacDonald) > 7. RE: Freelance Histology (Patsy Ruegg) > 8. GSH Histopalooza (David Kemler) > 9. Re: Need very good used microscope (Rene J Buesa) > 10. AUTO: Bruce Palmatier is out of the office (returning > 04/25/2014) (Bruce_Palmatier@vwr.com) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 22 Apr 2014 15:54:58 -0500 > From: > Subject: [Histonet] Need a Laboratory/Medical Assistant > To: > Message-ID: <006601cf5e6d$1ff01e30$5fd05a90$@integrityhealthplus.com> > Content-Type: text/plain; charset="us-ascii" > > Hello Histology land, > > > > I have an opening for a full time position Monday-Friday for a > Laboratory Assistant/Medical Assistant. This person will help out > approximately three days a week in our laboratory in Farmers Branch > (close to Addison), Tx. and two days a week at a doctor's office for > specimen collection (one in town office). They must have reliable > transportation. If you know of anyone interested in this position or > finding out more information about this position please email me, Gloria, at Gloria@integrityhealthplus.com . > Thank > you > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 22 Apr 2014 20:58:16 +0000 > From: Patricia F Lott > Subject: [Histonet] need Linda Jenkins > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <372C4AF089B6AE48B36F064FA891FF7813896328@UABEXMB3.ad.uab.edu> > Content-Type: text/plain; charset="us-ascii" > > Someone please send me the contact info for Linda Jenkins, formerly of > Clemson University. > > > ------------------------------ > > Message: 3 > Date: Tue, 22 Apr 2014 18:07:28 -0400 > From: H R > Subject: [Histonet] Freelance Histology > To: histonet@lists.utsouthwestern.edu > Message-ID: > 0vkVTwe8yg@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Has anyone heard of people cutting and staining slides out of your > garage or some kind of similar set up, like picking up overflow from > different companies /labs? I overheard a conversation about someone > doing this in their retirement. Labs send them the block and all they > do is cut and do HE stain and return both and bill? What kinds of > regulations would that entail? I don't know if that could be true. Maybe for research facilities? > > -- > *H* > > > ------------------------------ > > Message: 4 > Date: Tue, 22 Apr 2014 16:23:26 -0600 > From: Elizabeth Chlipala > Subject: RE: [Histonet] Freelance Histology > To: H R , "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <14E2C6176416974295479C64A11CB9AE019C79E05971@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="utf-8" > > Cutting is one thing such as like control slides, but staining > thatC"b,b"s a whole different thing, you will have chemical waste > that needs to be disposed of properly. You will need appropriate ventilation, etc. > Correct me if I am wrong but I donC"b,b"t think you could be > performing any clinical work, it would have to be research. Even > though you might be only one person I still think you are responsible > for all applicable OSHA guidelines. > > Just my two cents. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R > Sent: Tuesday, April 22, 2014 4:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Freelance Histology > > Has anyone heard of people cutting and staining slides out of your > garage or some kind of similar set up, like picking up overflow from > different companies /labs? I overheard a conversation about someone > doing this in their retirement. Labs send them the block and all they > do is cut and do HE stain and return both and bill? What kinds of > regulations would that entail? I don't know if that could be true. Maybe for research facilities? > > -- > *H* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 5 > Date: Tue, 22 Apr 2014 16:28:09 -0600 > From: Elizabeth Chlipala > Subject: RE: [Histonet] Freelance Histology > To: Elizabeth Chlipala , H R > , "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <14E2C6176416974295479C64A11CB9AE019C79E05973@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="utf-8" > > Please excuse my grammar, yikes. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth > Chlipala > Sent: Tuesday, April 22, 2014 4:23 PM > To: H R; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Freelance Histology > > Cutting is one thing such as like control slides, but staining > thatC"b,b"s a whole different thing, you will have chemical waste > that needs to be disposed of properly. You will need appropriate ventilation, etc. > Correct me if I am wrong but I donC"b,b"t think you could be > performing any clinical work, it would have to be research. Even > though you might be only one person I still think you are responsible > for all applicable OSHA guidelines. > > Just my two cents. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box > 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of H R > Sent: Tuesday, April 22, 2014 4:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Freelance Histology > > Has anyone heard of people cutting and staining slides out of your > garage or some kind of similar set up, like picking up overflow from > different companies /labs? I overheard a conversation about someone > doing this in their retirement. Labs send them the block and all they > do is cut and do HE stain and return both and bill? What kinds of > regulations would that entail? I don't know if that could be true. Maybe for research facilities? > > -- > *H* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 6 > Date: Tue, 22 Apr 2014 15:43:04 -0700 > From: Jennifer MacDonald > Subject: [Histonet] California Society for Histotechnology > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > OFC789EC65.3D8780F0-ON88257CC2.007C7DDA-88257CC2.007CCA4F@mtsac.edu> > Content-Type: text/plain; charset="US-ASCII" > > Don't miss out on some great work shops. The deadline for > registration has been extended to this Friday and if you still need a > room that can be arranged. > > For registration: > http://californiahistology.org/events.html > > For rooms: > contact Hilton reservations coordinator Yvette Moradians at > 818-596-4506 > > ------------------------------ > > Message: 7 > Date: Tue, 22 Apr 2014 18:25:40 -0600 > From: Patsy Ruegg > Subject: RE: [Histonet] Freelance Histology > To: "liz@premierlab.com" , H R > , "Histonet@Lists. Edu" > > Message-ID: > Content-Type: text/plain; charset="Windows-1252" > > this may certainly not be on the up and up but I have heard about > these kinds of operations for years, not sure how they can do it but I > know of a lab that did clinical skin bx samples for years and years > and I am sure they were not Clia/CAP/or even GLP, I guess since it is > Histology all the responsibility lies with the Pathologist reading the > slides, I never thought it was very kosher, at least for this one case > it is not going on anymore, but it would not surprise me if there were > Derm and GI labs all over not really accredited to be doing histology > on human samples but they still do. > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > pruegg@ihctech.net > > > From: liz@premierlab.com > To: liz@premierlab.com; hrfulklab@gmail.com; > histonet@lists.utsouthwestern.edu > Date: Tue, 22 Apr 2014 16:28:09 -0600 > Subject: RE: [Histonet] Freelance Histology > CC: > > Please excuse my grammar, yikes. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO > Box 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth > Chlipala > Sent: Tuesday, April 22, 2014 4:23 PM > To: H R; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Freelance Histology > > Cutting is one thing such as like control slides, but staining thatb From TanyaAbbott <@t> catholichealth.net Thu Apr 24 12:58:59 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Thu Apr 24 12:59:19 2014 Subject: [Histonet] Combo xylene/xylene substitute Message-ID: <852F7D2C14FB464D80E182B15DB138AF306786E3@CHIEX005.CHI.catholichealth.net> We use xylene substitute for everything in our lab instead of xylene, including in our Prisma stainer. We are looking to purchase the coverslipper and link, which needs to use xylene with the coverslipper tape, and also in the last station of the stainer. Has anyone ever had to use both xylene and xylene substitute (Citra-clear) in their staining process? Any suggestions? Thanks! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From jwell1958 <@t> msn.com Thu Apr 24 13:00:30 2014 From: jwell1958 <@t> msn.com (Janci Wellborn) Date: Thu Apr 24 13:00:39 2014 Subject: [Histonet] Missouri Annual Meeting Message-ID: It is not too late to have a wonderful weekend with other histotechs at a spa in Branson, Mo! Come and get some continuing education with us in the beautiful hills! There is something for everyone. Studying for the HT/HTL ASCP Exam - come to the review! In research - numerous workshops just for you. Want to brush up on your molecular knowledge? There is some of that also. The link to the registration is https://s3.goeshow.com/nsh/2014MSS/ereg403598.cfm?clear Janci Wellborn, BS, HTL(ASCP) President, Missouri Society for Histotechnology From mpence <@t> grhs.net Thu Apr 24 13:10:31 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Apr 24 13:10:55 2014 Subject: [Histonet] RE: Pathology Report Templates In-Reply-To: References: Message-ID: Can someone site the CAP check list question # that applies to this? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, April 24, 2014 12:54 PM To: 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pathology Report Templates This is a fact, to meet compliance the signing facility needs to be ID along with the CLIA#, ,haven't heard about the medical director issue, but who knows -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Thursday, April 24, 2014 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology Report Templates One of our facilities had a CAP inspection last week and I was asked to add the CLIA# and Medical Director to the template, immediately. Is this something new, as we had our CAP inspection last Aug. and there was no mention about CLIA# or having the Director's name on the templates? I haven't been able to get any follow-up from the lab supervisor. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Thu Apr 24 13:26:06 2014 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Apr 24 13:26:16 2014 Subject: [Histonet] RE: I need opinions on Leica instruments In-Reply-To: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> References: <09FBA01CA9B6374A83C5C76E09E46188125E5F80@EXMAIL1-FHC.firsthealth.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E022B3F9D@HHCEXCHMB03.hhcsystem.org> We have two Peloris processors in our Histology Laboratory and we run them around the clock. In addition, I have been using the Bond automated IHC platform in my Immunopathology/Morphologic Proteomics Laboratory since 2007. We now have 4 Bond Max instruments and 1 Bond III; absolutely incredible machines. I would not trade them for anything; not even for two more College National Basketball Championships! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.cartun@hhchealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Duddey, Aimee Sent: Thursday, April 24, 2014 7:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I need opinions on Leica instruments I am considering the Peloris II tissue processor and the Bond IHC stainer for our lab. We are a 400 bed regional hospital with a growing outreach business. We have had Thermo Excelsior processor for years and have never really been happy with it. Of course our VIP is as reliable and consistent as they come. We currently have a full Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any feedback about Leica's line of products. Please include experiences good and bad. Thank you in advance. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FirstHealth Moore Regional Hospital Pinehurst, NC 28374 (910) 715-5286 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From rooster3801 <@t> yahoo.com Thu Apr 24 14:08:18 2014 From: rooster3801 <@t> yahoo.com (Rooster) Date: Thu Apr 24 14:08:33 2014 Subject: [Histonet] I need opinions on Leica instruments In-Reply-To: References: Message-ID: <49AA0871-68F8-4CF7-B059-A043701D5880@yahoo.com> Love them also! Chris On Apr 24, 2014, at 9:11 AM, "Britton, Josette C" wrote: > I love all of their instruments! Josie Britton, HT (ASCP) QIHC > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Duddey, > Aimee > Sent: Thursday, April 24, 2014 7:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] I need opinions on Leica instruments > > I am considering the Peloris II tissue processor and the Bond IHC > stainer for our lab. We are a 400 bed regional hospital with a growing > outreach business. We have had Thermo Excelsior processor for years and > have never really been happy with it. Of course our VIP is as reliable > and consistent as they come. We currently have a full Ventana line for > IHC (2 ultras, 2 XTs, and BMK special stainer). I would appreciate any > feedback about Leica's line of products. Please include experiences > good and bad. > Thank you in advance. > > Aimee M. Duddey, MLT(ASCP) > Assistant Director of Laboratory - Pathology > FirstHealth Moore Regional Hospital > Pinehurst, NC 28374 > (910) 715-5286 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena <@t> statlab.com Thu Apr 24 15:47:29 2014 From: DSiena <@t> statlab.com (Debra Siena) Date: Thu Apr 24 15:47:30 2014 Subject: [Histonet] Retinal detachment In-Reply-To: <49AA0871-68F8-4CF7-B059-A043701D5880@yahoo.com> References: <49AA0871-68F8-4CF7-B059-A043701D5880@yahoo.com> Message-ID: Hi All, I have a friend that needs help to prevent retinal detachment in mouse eyes. Her question is below. Thanks for any help that you can provide. "We have tried Davidson's fixative before, it is ok. What we are trying to do is eliminate the retinal detachment, wherever it comes from (either the harvesting technique, fixative, or processing). Will you ask how long do we fix mouse and rat eyes in each of the fixatives (alcoholic formalin, zinc formalin and Davidson's). After fixation, we transfer to 70% ethanol, and it starts out on the processor in 70% ethanol for about a 7 hour processing program. Get back to me as soon as you can. Thank you very much. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rooster Sent: Thursday, April 24, 2014 2:08 PM To: Britton, Josette C Cc: ; Duddey, Aimee Subject: Re: [Histonet] I need opinions on Leica instruments Love them also! Chris On Apr 24, 2014, at 9:11 AM, "Britton, Josette C" wrote: > I love all of their instruments! Josie Britton, HT (ASCP) QIHC > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Duddey, Aimee > Sent: Thursday, April 24, 2014 7:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] I need opinions on Leica instruments > > I am considering the Peloris II tissue processor and the Bond IHC > stainer for our lab. We are a 400 bed regional hospital with a > growing outreach business. We have had Thermo Excelsior processor for > years and have never really been happy with it. Of course our VIP is > as reliable and consistent as they come. We currently have a full > Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I > would appreciate any feedback about Leica's line of products. Please > include experiences good and bad. > Thank you in advance. > > Aimee M. Duddey, MLT(ASCP) > Assistant Director of Laboratory - Pathology FirstHealth Moore > Regional Hospital Pinehurst, NC 28374 > (910) 715-5286 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Apr 24 16:00:37 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Apr 24 16:00:42 2014 Subject: [Histonet] RE: Retinal detachment In-Reply-To: References: <49AA0871-68F8-4CF7-B059-A043701D5880@yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0599D@SBS2K8.premierlab.local> Debra In order for the retina to remain attached you MUST fix in Davidsons, 10% NBF and 4% PFA are not acceptable fixatives they will cause retinal detachment. You also need to trim the eye on one edge prior to processing or it's just going to shrink up like a raisin and look like hell. We section a lot of eye samples and it can be a bit tricky. We do not collect the samples - they are received in 70% alcohol we trim one edge and process on a shorter processing cycle (around 20 minutes a station, same day processing) with 3 changes of Propar instead of xylene. Good Luck - eyes can be tricky! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Thursday, April 24, 2014 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Retinal detachment Hi All, I have a friend that needs help to prevent retinal detachment in mouse eyes. Her question is below. Thanks for any help that you can provide. "We have tried Davidson's fixative before, it is ok. What we are trying to do is eliminate the retinal detachment, wherever it comes from (either the harvesting technique, fixative, or processing). Will you ask how long do we fix mouse and rat eyes in each of the fixatives (alcoholic formalin, zinc formalin and Davidson's). After fixation, we transfer to 70% ethanol, and it starts out on the processor in 70% ethanol for about a 7 hour processing program. Get back to me as soon as you can. Thank you very much. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rooster Sent: Thursday, April 24, 2014 2:08 PM To: Britton, Josette C Cc: ; Duddey, Aimee Subject: Re: [Histonet] I need opinions on Leica instruments Love them also! Chris On Apr 24, 2014, at 9:11 AM, "Britton, Josette C" wrote: > I love all of their instruments! Josie Britton, HT (ASCP) QIHC > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Duddey, Aimee > Sent: Thursday, April 24, 2014 7:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] I need opinions on Leica instruments > > I am considering the Peloris II tissue processor and the Bond IHC > stainer for our lab. We are a 400 bed regional hospital with a > growing outreach business. We have had Thermo Excelsior processor for > years and have never really been happy with it. Of course our VIP is > as reliable and consistent as they come. We currently have a full > Ventana line for IHC (2 ultras, 2 XTs, and BMK special stainer). I > would appreciate any feedback about Leica's line of products. Please > include experiences good and bad. > Thank you in advance. > > Aimee M. Duddey, MLT(ASCP) > Assistant Director of Laboratory - Pathology FirstHealth Moore > Regional Hospital Pinehurst, NC 28374 > (910) 715-5286 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Thu Apr 24 19:54:57 2014 From: suetp918 <@t> comcast.net (Sue) Date: Thu Apr 24 19:55:28 2014 Subject: [Histonet] RE: competency form In-Reply-To: <002401cf53ed$f57c6ac0$e0754040$@wisc.edu> Message-ID: <884148147.1449772.1398387297412.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> We have the same issue. It is bad when they try to use the same QA form for out of range proficiency testing. a +3 or +4 is IHC is not the same as 70 or 200 glucose result. The pathologists are haven real issues with it as well. S. Paturzo From carl.hobbs <@t> kcl.ac.uk Thu Apr 24 22:01:48 2014 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Apr 24 22:01:55 2014 Subject: [Histonet] Re: Posting Images for Histonet (Marvin Hanna) Message-ID: <2a968d89aea64d7d9e21b7167e839893@AM3PR03MB626.eurprd03.prod.outlook.com> Looks a great idea;?simple to use. Thanks. I used to post images on the impressive ?Immunoportal website but, that is sadly no more. Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From christina.kreutzer01 <@t> gmail.com Fri Apr 25 01:58:52 2014 From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer) Date: Fri Apr 25 01:58:59 2014 Subject: [Histonet] cryosectioning, bubbles between slide and section Message-ID: Hello members, I would need some help regarding cryosectioning of spinal cord. I am currently establishing cryosectioning on the cryostat (Leica CM1950) for this tissue and am having some problems. I am cutting 4% PFA fixed and succrose infiltrated spinal cords, embedded in Tissue Tek at 10?m and a temperature of approximately -16?C . I get more or less smoth slices but once I let them adhere to the slide -directly from the knife, after straightening it carefully with a brush - I get massive air bubbles between the slide and the slice. I have experience cutting on cryostats and with different tissues and never have had this problem before. I tried to change the temperature of the chamber and/or chunk and tried to warm the slide before adhering the slice and I tried to cool the slide. But it didn't help. Do you think changing from 30% succrose to a mixture of Tissue Tek/Succrose or even Tissue Tek/PBS would help? Does anybody have an advice? Regards Christina From mpence <@t> grhs.net Fri Apr 25 08:00:00 2014 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Apr 25 08:00:08 2014 Subject: [Histonet] RE: Pathology Report Templates In-Reply-To: References: Message-ID: I went through all my CAP check list questions and I do not see this anywhere. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, April 24, 2014 12:54 PM To: 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pathology Report Templates This is a fact, to meet compliance the signing facility needs to be ID along with the CLIA#, ,haven't heard about the medical director issue, but who knows -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Thursday, April 24, 2014 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology Report Templates One of our facilities had a CAP inspection last week and I was asked to add the CLIA# and Medical Director to the template, immediately. Is this something new, as we had our CAP inspection last Aug. and there was no mention about CLIA# or having the Director's name on the templates? I haven't been able to get any follow-up from the lab supervisor. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Fri Apr 25 10:25:06 2014 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Apr 25 10:25:12 2014 Subject: [Histonet] RE: Pathology Report Templates In-Reply-To: References: Message-ID: This is for the MU (meaningful use) -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, April 25, 2014 6:00 AM To: Jesus Ellin; 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu Subject: RE: Pathology Report Templates I went through all my CAP check list questions and I do not see this anywhere. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, April 24, 2014 12:54 PM To: 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pathology Report Templates This is a fact, to meet compliance the signing facility needs to be ID along with the CLIA#, ,haven't heard about the medical director issue, but who knows -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Thursday, April 24, 2014 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology Report Templates One of our facilities had a CAP inspection last week and I was asked to add the CLIA# and Medical Director to the template, immediately. Is this something new, as we had our CAP inspection last Aug. and there was no mention about CLIA# or having the Director's name on the templates? I haven't been able to get any follow-up from the lab supervisor. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From trathborne <@t> somerset-healthcare.com Fri Apr 25 11:24:32 2014 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Apr 25 11:26:05 2014 Subject: [Histonet] RE: Pathology Report Templates In-Reply-To: References: Message-ID: <3AD061FE740D464FAC7BF6B5CFB75707A95CF5FD@SMCMAIL01.somerset-healthcare.com> Can you cite a source for the MU requirement? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Friday, April 25, 2014 11:25 AM To: 'Mike Pence'; 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pathology Report Templates This is for the MU (meaningful use) -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, April 25, 2014 6:00 AM To: Jesus Ellin; 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu Subject: RE: Pathology Report Templates I went through all my CAP check list questions and I do not see this anywhere. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Thursday, April 24, 2014 12:54 PM To: 'Victor A. Tobias'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pathology Report Templates This is a fact, to meet compliance the signing facility needs to be ID along with the CLIA#, ,haven't heard about the medical director issue, but who knows -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor A. Tobias Sent: Thursday, April 24, 2014 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology Report Templates One of our facilities had a CAP inspection last week and I was asked to add the CLIA# and Medical Director to the template, immediately. Is this something new, as we had our CAP inspection last Aug. and there was no mention about CLIA# or having the Director's name on the templates? I haven't been able to get any follow-up from the lab supervisor. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LLoss <@t> dermwisconsin.com Fri Apr 25 12:06:53 2014 From: LLoss <@t> dermwisconsin.com (Lee Loss) Date: Fri Apr 25 12:07:01 2014 Subject: [Histonet] IHC billing Message-ID: <2B99CB40EC5CC7409888A2961CBF96884E815196@EX2010.DERM.LOCAL> Hi all, I know this topic has been tossed around a bit already. We are having some interpretation issues with one insurance provider on billing for IHC using the CPT codes 88342 & 88343. Our interpretation is to use the 88342 for the first uniquely identifiable antibody per slide and 88343 for each additional antibody per slide. This particular large insurance company is telling us (and reimbursing us accordingly) that they will reimburse for the first antibody per case and 88343 for any thereafter, in spite of the language of the new AMA guidelines that they quote within their own policies. How are other labs interpreting this new rule and has anyone else had issues with insurance companies using different interpretations? They also want us to explain to them why it is necessary to use separate slides for additional antibodies as opposed to applying all of the antibodies to one slide. I believe I have answered that question for them but would love to hear thoughts on that as well. I'm sure there are some really good points that I haven't thought of. Thanks in advance! Lee Dermatology Associates ________________________________ The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. From Joyce.Weems <@t> emoryhealthcare.org Fri Apr 25 13:55:28 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Apr 25 13:55:38 2014 Subject: [Histonet] PAM4 Antibody Message-ID: Hello Everybody, Does anyone do this ab? If so, where do you get it? TIA - have a great weekend! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Ronald.Houston <@t> nationwidechildrens.org Sat Apr 26 00:21:25 2014 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Sat Apr 26 00:21:42 2014 Subject: [Histonet] Specialist II position opening Message-ID: Due to growth, Nationwide Children's Hospital, Columbus, Ohio is pleased to open a new position for a Specialist II in the Department of Anatomic Pathology. We are looking for an HT/HTL or MT, with at least a BS and a minimum of eight (8) years experience in all aspects of histotechnology in a clinical and/or research laboratory. The Specilaist II is responsible for overseeing and maintaining the technical and procedural operations of the department; researching, developing and implementing new or improved methodologies and instrumentation; performing and monitoring quality assurance, quality improvement and regulatory compliance tasks; and will directly report to and support the anatomic pathology manager. You will have the opportunity to work not only in one of the top pediatric facilities in the world but also, we believe, one of the premier anatomic pathology departments. We are a forward thinking department determined to achieve the best outcomes for our children, constantly seeking to adapt and improve the services we provide, and increasing the diagnostic, prognostic and predictive testing available. Nationwide Children's is a place where staff are fully engaged, and a place that the staff are truly proud to be a part of.. We invite suitable candidtaes to check us out and apply online at http://nationwidechildrens.org and you can find out more about the outstanding care our kids get at http://700childrens.nationwidechildrens.org/ For more information about the position, please contact Ronnie Houston at ronald.houston@nationwidechildrens.org and/or (614) histonet7225450 From hrumbut <@t> yahoo.com Sun Apr 27 15:50:34 2014 From: hrumbut <@t> yahoo.com (Heather R) Date: Sun Apr 27 15:50:39 2014 Subject: [Histonet] Control Blocks Message-ID: <1398631834.32796.YahooMailNeo@web141203.mail.bf1.yahoo.com> Anyone know where I can purchase positive control blocks for special stains? I'm in an independent lab and don't have access to hospital cases. Thanks From histo <@t> skm.org.pk Mon Apr 28 07:26:41 2014 From: histo <@t> skm.org.pk (Pathology-Histology Sr. Supervisor) Date: Mon Apr 28 07:24:18 2014 Subject: [Histonet] company for antibody HSV(cocktail) on paraffin section Message-ID: Hi All We are looking company for antibody HSV(cocktail) on paraffin section, kindly any comments. Regards Muhammad Tahseen Histology supervisor SKMCH&RC Lahore From amurvosh <@t> advancederm.net Mon Apr 28 09:16:30 2014 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Mon Apr 28 09:16:37 2014 Subject: [Histonet] company for antibody HSV(cocktail) on paraffin section In-Reply-To: References: Message-ID: <4AD6A4E531E8C943A730559B6B81DF07D759F4@dc.Advancederm.net> Biocare has a herpes I&II mix if that's what you're looking for. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathology-Histology Sr. Supervisor Sent: Monday, April 28, 2014 5:27 AM To: histonet@lists.utsouthwestern.edu Cc: tahseen0009@gmail.com Subject: [Histonet] company for antibody HSV(cocktail) on paraffin section Hi All We are looking company for antibody HSV(cocktail) on paraffin section, kindly any comments. Regards Muhammad Tahseen Histology supervisor SKMCH&RC Lahore _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From danielle <@t> ka-recruiting.com Mon Apr 28 10:33:07 2014 From: danielle <@t> ka-recruiting.com (Danielle Lareau) Date: Mon Apr 28 10:33:30 2014 Subject: [Histonet] Exciting Histotech Opportunities Message-ID: <1829883259.1398699186865.JavaMail.cfservice@sl14app2> Histonet Community, I hope all is well; I am a recruiter at a Healthcare Recruiting Firm, KA Recruiting, and specialize in the placement of lab professionals in permanent positions across the country. I wanted to check in and see if you were interested in some of the positions that I am currently working on. Our clients offer competitive compensation packages, and typically assist with relocation expenses. I currently have two great Histotech positions that may be of interest to you available at a hospital near Albany, NY. The 160 bed hospital is looking to fill two permanent, full time day shift positions; a Histotechnologist as well as a Technical Specialist, which is more of a senior histotech/leadership role. Both positions require HT(ASCP) or HTL(ASCP) certification, and all candidates must have a current NYS licensure. We also have some other histotech opportunities that we are working on, listed below. Please let me know if you are interested in any of these openings by sending an updated resume to danielle@ka-recruiting.com as well as a good time to reach you and go over what you are looking for in a new position as well as your past experience, education, and certifications. Histotech Positions: - Histology Supervisor, New York - Histotech, New York - Histotech I, II, and III, New York - Grossing Technician, New York - Senior Lab Technician, Pathology, Ohio - Histology Supervisor, North Carolina If you are interested in any other laboratory opportunities outside of these geographic locations, please feel free to send an updated resume with your geographic preferences as well as any other types of positions you may be interested in. I would be happy to let you know about any opportunities that come up in the future, as we work with clients all over the country. Our services are free of charge for all candidates, and we also offer a referral bonus for anyone who you refer to us that we place in any position, recommendations on other candidates you may know are welcome! To view some additional opportunities please visit our website at www.ka-recruiting.com . Sincerely, Danielle Lareau K.A. Recruiting Inc. 10 Post Office Square, 8th Floor South Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 danielle@ka-recruiting.com www.karecruiting.com From Sarah.Dysart <@t> stdavids.com Mon Apr 28 10:42:44 2014 From: Sarah.Dysart <@t> stdavids.com (Sarah.Dysart@stdavids.com) Date: Mon Apr 28 10:42:51 2014 Subject: [Histonet] PRN Position Message-ID: <34C22BB94729434598D767D3F4EB95E0AAB08BDF7D@FWDCWPMSGCMS03.hca.corpad.net> We have an opening for a PRN histotech. It would be to fill in when people take time off. Hours would be from 4 or 5 to 7 or 8 (but we can work around those). Please go to the St. David's website (www.stdavids.com) to apply, or contact me directly. Thank you, Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 From TanyaAbbott <@t> catholichealth.net Mon Apr 28 11:20:18 2014 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Apr 28 11:20:23 2014 Subject: [Histonet] Tissue Tek Prisma coverslipper Message-ID: <852F7D2C14FB464D80E182B15DB138AF3067ABE7@CHIEX005.CHI.catholichealth.net> Does anyone who uses the Tissue Tek Prisma coverslipper have an external ventilation source? Or is the internal filtering system the instrument uses good enough? Thanks! Tanya Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabbott@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. From KSimeone <@t> leavittmgt.com Mon Apr 28 11:40:08 2014 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon Apr 28 11:40:15 2014 Subject: [Histonet] BioVision Autostanier RsT Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C01A417@vm-email.leavittmgt.com> Hi all! Does anyone have a PAS-F protocol for the old BioVision Autostainer they can share? We have one but the pathologist is complaining there isn't enough differentiation (too blue). It currently runs for 10 min in Periodic Acid & 20 minutes in Schiff's. I would appreciate it! Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From vtobias <@t> uw.edu Mon Apr 28 13:22:53 2014 From: vtobias <@t> uw.edu (Victor A. Tobias) Date: Mon Apr 28 13:24:26 2014 Subject: [Histonet] Pathology Report Templates In-Reply-To: References: Message-ID: Here is the explanation from the CAP inspector, not a requirement yet. Okay I just talked to Anita about why the inspector wanted CLIA number and Director Name on report and the reason why is that the inspector said that patients are now having easier access to their reports, and laboratories are placing this information on the reports for references, and this is something that will be necessary in the future, and he for some reason wanted to see that we were going to do this. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ________________________________ From: Burke Reed [mailto:burke.3@hotmail.com] Sent: Thursday, April 24, 2014 12:24 PM To: Victor A. Tobias Subject: RE: [Histonet] Pathology Report Templates I reviewed the 2014 CAP checklist twice. I see no mention of a requirement for CLIA# and Medical Director to be on the final pathology report. Burke Reed, III > From: vtobias@uw.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 24 Apr 2014 17:50:30 +0000 > Subject: [Histonet] Pathology Report Templates > > One of our facilities had a CAP inspection last week and I was asked to add the CLIA# and Medical Director to the template, immediately. Is this something new, as we had our CAP inspection last Aug. and there was no mention about CLIA# or having the Director's name on the templates? > > I haven't been able to get any follow-up from the lab supervisor. > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB244 > Seattle, WA 98104 > vtobias@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Mon Apr 28 14:59:27 2014 From: akemiat3377 <@t> yahoo.com (Akemi Allison) Date: Mon Apr 28 14:59:32 2014 Subject: [Histonet] Was told I am having bounce-backs Message-ID: <1398715167.51751.YahooMailNeo@web140606.mail.bf1.yahoo.com> This is a test to see if this posts on histonet.? I was told yesterday that there are a number of bounce-backs. I haven't received emails from histonet the past few days.? Anyone else having problems?? Peculier issues with yahoo lately Akemi Allison-Tacha, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 W. Email: aallison@montereygi.com P. Email: akemiat@yahoo.com???? From PAMarcum <@t> uams.edu Mon Apr 28 15:06:48 2014 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Mon Apr 28 15:06:54 2014 Subject: [Histonet] Was told I am having bounce-backs In-Reply-To: <1398715167.51751.YahooMailNeo@web140606.mail.bf1.yahoo.com> References: <1398715167.51751.YahooMailNeo@web140606.mail.bf1.yahoo.com> Message-ID: <41D3A1AF6FEF0643BDC89E0516A6EA32010DFBBBB0@Mail2Node2.ad.uams.edu> I have had the same issue and was told by HistoNet about the problem of bounces. I did get some strange e-mails coming through on HistoNet that I deleted. The problem was they opened in my end mail box due to my view settings before I could get rid of them. I am wondering if this was the problem as I recognized no one on the bounced list I received. I have gotten e-mails today. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Monday, April 28, 2014 2:59 PM To: Histonet Subject: [Histonet] Was told I am having bounce-backs This is a test to see if this posts on histonet.? I was told yesterday that there are a number of bounce-backs. I haven't received emails from histonet the past few days.? Anyone else having problems?? Peculier issues with yahoo lately Akemi Allison-Tacha, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 W. Email: aallison@montereygi.com P. Email: akemiat@yahoo.com???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From contact <@t> excaliburpathology.com Mon Apr 28 15:15:05 2014 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Apr 28 15:15:09 2014 Subject: [Histonet] Was told I am having bounce-backs In-Reply-To: <41D3A1AF6FEF0643BDC89E0516A6EA32010DFBBBB0@Mail2Node2.ad.uams.edu> References: <1398715167.51751.YahooMailNeo@web140606.mail.bf1.yahoo.com> <41D3A1AF6FEF0643BDC89E0516A6EA32010DFBBBB0@Mail2Node2.ad.uams.edu> Message-ID: <1398716105.71844.YahooMailNeo@web162504.mail.bf1.yahoo.com> I have received the same message. The problem may be that my website is hosted by yahoo servers, even though yahoo is not in the name. Many facilities are blocking anything from yahoo, gmail, etc. because of potential viruses. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ From: "Marcum, Pamela A" To: 'Akemi Allison' ; Histonet Sent: Monday, April 28, 2014 3:06 PM Subject: RE: [Histonet] Was told I am having bounce-backs I have had the same issue and was told by HistoNet about the problem of bounces.? I did get some strange e-mails coming through on HistoNet that I deleted.? The problem was they opened in my end mail box due to my view settings before I could get rid of them.? I am wondering if this was the problem as I recognized no one on the bounced list I received.? I have gotten e-mails today.? Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Monday, April 28, 2014 2:59 PM To: Histonet Subject: [Histonet] Was told I am having bounce-backs This is a test to see if this posts on histonet.? I was told yesterday that there are a number of bounce-backs. I haven't received emails from histonet the past few days.? Anyone else having problems?? Peculier issues with yahoo lately Akemi Allison-Tacha, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 W. Email: aallison@montereygi.com P. Email: akemiat@yahoo.com???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Mon Apr 28 15:21:46 2014 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Mon Apr 28 15:22:03 2014 Subject: [Histonet] Was told I am having bounce-backs In-Reply-To: <1398716105.71844.YahooMailNeo@web162504.mail.bf1.yahoo.com> Message-ID: <1851844287.614780.1398716506935.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> We don't use Yahoo and the servers are not linked to a service outside the Universi ty as message system.? ----- Original Message ----- From: "Paula Pierce" To: "Pamela A Marcum" , "Akemi Allison" , "Histonet" Sent: Monday, April 28, 2014 3:15:05 PM Subject: Re: [Histonet] Was told I am having bounce-backs I have received the same message. The problem may be that my website is hosted by yahoo servers, even though yahoo is not in the name. Many facilities are blocking anything from yahoo, gmail, etc. because of potential viruses. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ ?From: "Marcum, Pamela A" To: 'Akemi Allison' ; Histonet Sent: Monday, April 28, 2014 3:06 PM Subject: RE: [Histonet] Was told I am having bounce-backs ? I have had the same issue and was told by HistoNet about the problem of bounces.? I did get some strange e-mails coming through on HistoNet that I deleted.? The problem was they opened in my end mail box due to my view settings before I could get rid of them.? I am wondering if this was the problem as I recognized no one on the bounced list I received.? I have gotten e-mails today.? Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Monday, April 28, 2014 2:59 PM To: Histonet Subject: [Histonet] Was told I am having bounce-backs This is a test to see if this posts on histonet.? I was told yesterday that there are a number of bounce-backs. I haven't received emails from histonet the past few days.? Anyone else having problems?? Peculier issues with yahoo lately Akemi Allison-Tacha, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 W. Email: aallison@montereygi.com P. Email: akemiat@yahoo.com???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From william <@t> schuylerhouse.com Mon Apr 28 15:55:49 2014 From: william <@t> schuylerhouse.com (William Shipley) Date: Mon Apr 28 15:55:13 2014 Subject: [Histonet] Pathology Report Templates In-Reply-To: References: Message-ID: <70FE5F42CAEF4D10BAD134666AA55D7B@WilliamPC> As an LIS company we frequently see inspectors making up requirements. Sometimes it makes sense to appeal them sometimes it's easier to do what they want even if it isn't an actual requirement. I will admit that I had seen medical directors listed on laboratory reports so often that I found the idea that it was required plausible. I checked my copy of CLIA when the first post was made and was surprised to find that it wasn't there. The most common confusion is between the requirements for test reports (493.1291) and requirements for test records (493.1283). I would suggest that the advent of patients having the right to get their lab report would be an argument against the medical director being listed as this is probably not the person that the patient should be calling for an explanation of their report. This is just my opinion, of course. William Shipley Schuyler House ----- Original Message ----- From: "Victor A. Tobias" To: "Burke Reed" Cc: Sent: Monday, April 28, 2014 11:22 AM Subject: RE: [Histonet] Pathology Report Templates Here is the explanation from the CAP inspector, not a requirement yet. Okay I just talked to Anita about why the inspector wanted CLIA number and Director Name on report and the reason why is that the inspector said that patients are now having easier access to their reports, and laboratories are placing this information on the reports for references, and this is something that will be necessary in the future, and he for some reason wanted to see that we were going to do this. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtobias@u.washington.edu 206-744-2735 206-744-8240 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ________________________________ From: Burke Reed [mailto:burke.3@hotmail.com] Sent: Thursday, April 24, 2014 12:24 PM To: Victor A. Tobias Subject: RE: [Histonet] Pathology Report Templates I reviewed the 2014 CAP checklist twice. I see no mention of a requirement for CLIA# and Medical Director to be on the final pathology report. Burke Reed, III > From: vtobias@uw.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 24 Apr 2014 17:50:30 +0000 > Subject: [Histonet] Pathology Report Templates > > One of our facilities had a CAP inspection last week and I was asked to > add the CLIA# and Medical Director to the template, immediately. Is this > something new, as we had our CAP inspection last Aug. and there was no > mention about CLIA# or having the Director's name on the templates? > > I haven't been able to get any follow-up from the lab supervisor. > > Victor Tobias HT(ASCP) > Clinical Applications Analyst > Harborview Medical Center > Dept of Pathology Room NJB244 > Seattle, WA 98104 > vtobias@u.washington.edu > 206-744-2735 > 206-744-8240 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use of > the intended recipients. If you are not the intended recipient, or if the > message has been addressed to you in error, do not read, disclose, > reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and then > destroy all copies of the message and any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bbrinegarhtl <@t> gmail.com Mon Apr 28 16:56:16 2014 From: bbrinegarhtl <@t> gmail.com (Beth Brinegar) Date: Mon Apr 28 16:56:24 2014 Subject: [Histonet] Negative controls Message-ID: Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 From koellingr <@t> comcast.net Mon Apr 28 17:03:42 2014 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Apr 28 17:04:01 2014 Subject: a few words-Re: [Histonet] Was told I am having bounce-backs-off topic In-Reply-To: <1851844287.614780.1398716506935.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> References: <1851844287.614780.1398716506935.JavaMail.root@sz0001a.westchester.pa.mail.comcast.net> Message-ID: <728332955.35602938.1398722622167.JavaMail.root@comcast.net> I got a bounce a week ago and contacted Linda M.in private and was deemed fairly innocuous but as with all such things even remotely suspicious I deleted it and never, ever ?look into such things.? As it turns out, was talking a few days ago to a judge for my district STEM science fair who happens to be a computer security geek for an international company.? We were just discussing security failures in the Internet opening everyone, EVERYONE, up to fraud and disaster.? The Heart Bleed problem, just now Microsoft warned of its newly discovered hole in IE, the way hackers can now target companies through vending machines at work?that are hooked to company computers to monitor usage, the great security worry about the electrical infrastructure, targeting of dumb (I guess people who have them say smart) phones.? When I went to college and learned biology, food and water seemed to drive life and things like the Krebs citric acid cycle.? Now apparently digital gadgets are a part of actual life.? Not as a luxury or convenience but as part of life itself without which there is apparently death. As you sew, so shall you reap.? I hope we all can remember that evolution of our analog human brains with all sorts of cell signaling molecules has taken millions of years.? Can't be re-evolved in just a few years; if ever. Sent from my old, but hopefully safe, desktop PC, guarded by several layers of security walls. ? Ray in Seattle ----- Original Message ----- From: "Pam Marcum" To: "Paula Pierce" Cc: "Histonet" , "Pamela A Marcum" Sent: Monday, April 28, 2014 1:21:46 PM Subject: Re: [Histonet] Was told I am having bounce-backs We don't use Yahoo and the servers are not linked to a service outside the Universi ty as message system.? ----- Original Message ----- From: "Paula Pierce" To: "Pamela A Marcum" , "Akemi Allison" , "Histonet" Sent: Monday, April 28, 2014 3:15:05 PM Subject: Re: [Histonet] Was told I am having bounce-backs I have received the same message. The problem may be that my website is hosted by yahoo servers, even though yahoo is not in the name. Many facilities are blocking anything from yahoo, gmail, etc. because of potential viruses. ? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Please note new address! Norman, OK 73069 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ________________________________ ?From: "Marcum, Pamela A" To: 'Akemi Allison' ; Histonet Sent: Monday, April 28, 2014 3:06 PM Subject: RE: [Histonet] Was told I am having bounce-backs ? I have had the same issue and was told by HistoNet about the problem of bounces.? I did get some strange e-mails coming through on HistoNet that I deleted.? The problem was they opened in my end mail box due to my view settings before I could get rid of them.? I am wondering if this was the problem as I recognized no one on the bounced list I received.? I have gotten e-mails today.? Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison Sent: Monday, April 28, 2014 2:59 PM To: Histonet Subject: [Histonet] Was told I am having bounce-backs This is a test to see if this posts on histonet.? I was told yesterday that there are a number of bounce-backs. I haven't received emails from histonet the past few days.? Anyone else having problems?? Peculier issues with yahoo lately Akemi Allison-Tacha, BS, HT (ASCP) HTL Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 (381) 375-3577? X117 W. Email: aallison@montereygi.com P. Email: akemiat@yahoo.com???? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From debgranato <@t> yahoo.com Mon Apr 28 17:04:49 2014 From: debgranato <@t> yahoo.com (Debbie Granato) Date: Mon Apr 28 17:04:52 2014 Subject: [Histonet] DRS 601 slide stainer Message-ID: We recently purchased a DRS 601 slide stainer and we are trying to find 2 staining dishes and handles. We also need a manual. Can anyone help us? We will purchase them and pay shipping. Also, could anyone give us the humidity range for the laboratory? Thank you! Debbie Granato HT(ASCP ) Sent from my iPhone From Ronald.Houston <@t> nationwidechildrens.org Tue Apr 29 07:13:37 2014 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Apr 29 07:13:49 2014 Subject: [Histonet] new IHC billing and Sunquest CoPathPlus Message-ID: Besides manually entering IHC charges, has anyone worked out a way to automatically bill for 88342 and 88343 on cases in Sunquest CoPathPlus? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From LSebree <@t> uwhealth.org Tue Apr 29 07:32:43 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Apr 29 07:32:57 2014 Subject: [Histonet] Negative controls In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF0B2B09@UWHC-MBX12.uwhis.hosp.wisc.edu> We still run negative controls. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Apr 29 07:54:39 2014 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Tue Apr 29 07:54:44 2014 Subject: [Histonet] cryosectioning, bubbles between slide and section In-Reply-To: References: Message-ID: Hello, This may be a little late, but we use a mix of half 30% sucrose and half OCT to section. It works really well with 14 to 25 micron sections and no cooling or heating of the slide. Our tissue is very small though--embryonic spinal cord or brain from chick or mouse. I just change the way I pick it up to get rid of bubbles. Have you tried rolling the slide upwards when you pick it up (if that makes sense) as opposed to rolling it from left to right (or the opposite)? Also as our cryostat gets older, it tends to get more static and we cannot get rid of it. This causes the tissue to adhere to the slide faster, which definitely causes bubbles. That may the case, but I hope not!! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Fri, Apr 25, 2014 at 2:58 AM, Christina Kreutzer < christina.kreutzer01@gmail.com> wrote: > Hello members, > I would need some help regarding cryosectioning of spinal cord. I am > currently establishing cryosectioning on the cryostat (Leica CM1950) for > this tissue and am having some problems. I am cutting 4% PFA fixed and > succrose infiltrated spinal cords, embedded in Tissue Tek at 10?m and a > temperature of approximately -16?C . I get more or less smoth slices but > once I let them adhere to the slide -directly from the knife, after > straightening it carefully with a brush - I get massive air bubbles between > the slide and the slice. > > I have experience cutting on cryostats and with different tissues and never > have had this problem before. > > I tried to change the temperature of the chamber and/or chunk and tried to > warm the slide before adhering the slice and I tried to cool the slide. But > it didn't help. Do you think changing from 30% succrose to a mixture of > Tissue Tek/Succrose or even Tissue Tek/PBS would help? > Does anybody have an advice? > > Regards > Christina > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DSiena <@t> statlab.com Tue Apr 29 09:27:52 2014 From: DSiena <@t> statlab.com (Debra Siena) Date: Tue Apr 29 09:27:58 2014 Subject: [Histonet] Negative controls In-Reply-To: References: Message-ID: I just wanted to clarify there are two types of negative controls, one is a negative reagent control and one is a tissue that is known negative. A lot of time, we tend to focus on the negative reagent control and forget about the negative tissue control. I know that a lot of smart folks out there on histonet will be able to give you some direction as far as how to write a policy to cover both but just wanted to clarify that the two types of negative controls are different and the negative reagent control may be abandoned if you are running a polymer detection kit but the known negative tissue controls are still required. Thanks ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.436.1369 dsiena@statlab.com | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Apr 29 09:37:56 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Apr 29 09:38:11 2014 Subject: [Histonet] Negative controls In-Reply-To: References: Message-ID: This is what I put in my procedure shortly after Richard sent his email. There wasn't a problem at inspection. Following his announcement at NSH/ISH Forum in Windsor, CT, Dr. Richard Carten sent an email to Histonet on July 17, 2102, regarding CAP guidelines for negative controls. The checklist will be changed to reflect that negative controls will no longer be required for polymer based procedures. The new wording contains the following statement: ?Immunohistochemical tests using polymer-based systems (biotin-free) are sufficiently free of background reactivity to obviate the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director?. Dr. Stargel has approved the discontinuation of negative controls as of July 20, 2012. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 5:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From christina.kreutzer01 <@t> gmail.com Tue Apr 29 10:46:09 2014 From: christina.kreutzer01 <@t> gmail.com (Christina Kreutzer) Date: Tue Apr 29 10:46:16 2014 Subject: [Histonet] cryosectioning - still bubbles between slide and section, spinal cord Message-ID: Dear members, first I wanna thank all the people who responded to my first email (Andrea, Mesru and Emily).Your tips were very helpful. Unfortunately I am still fighting agains bubbles/ wrinkles/bubble-shaped wrinkles between my slices and the slide. During the last couple of days I tried: i) to adjust the temperature of the chamber and the sample, seems like -16?C brings the best slices ii) to cool the slide by putting it into the chamber and warming it with my thumb just the second I held it towards the slice to adher iii) to warm the slides by putting them on a 37?C warm heating-plate after attachement iv) to moisten the slides with a little bit of bidest before letting the slices attach and put these slides on a 37?C heating plate afterwards...that was kind of desperate try to straighten the slices slowely v) 3 different intermedia before embedding the sample in pure Tissue Tek, a) 30% Succrose, b) half 30% succrose and half Tissue Tek, c) half Tissue Tek half PBS but...nothing really helped. I still have wrinkles aka bubbles. Tomorrow I am going the cut perfusion fixed rat spinal cord, hoping that this makes a difference. Anyway, I think I have some options left 1) trying to cut on a different mikrotome and 2) Leica promised to send me some slide/embedding solution/blade samples that worked for other groups. Can anybody think of something I havent tried yet? Besides of raising slide thickness and cutting for freefloating procedure? I would be so thankful for any ideas/suggestions/tips! Regards Christina From Timothy.Morken <@t> ucsfmedctr.org Tue Apr 29 11:13:54 2014 From: Timothy.Morken <@t> ucsfmedctr.org (Morken, Timothy) Date: Tue Apr 29 11:14:06 2014 Subject: [Histonet] Your experience with barcoding and materials tracking in grossing and histology Message-ID: <761E2B5697F795489C8710BCC72141FF3677D39A@ex07.net.ucsf.edu> Hi all, I am giving a talk at NSH about implementing barcoding and materials tracking in histology. I have that experience with Cerner Copath AB&T, but not other systems. I'd like to hear from others, especially with other systems, about their experiences in implementing barcoding in their lab with their own LIS, third party vendors (ie, Vantanage, Cerebro, Lab Lion, Dako, others). Pros and cons, lessons learned, etc that would help others starting down that path. The point of the talk is the generalities of implementation, not a marketing talk on any particular system, though I would like to know why you chose a given system, whether you were able to use your own LIS for barcoding or had to use a third party vendor. Thanks in advance for any and all information. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org From tbraud <@t> holyredeemer.com Tue Apr 29 12:29:57 2014 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Apr 29 12:30:07 2014 Subject: [Histonet] Re: Negative Controls In-Reply-To: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> Message-ID: On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: "must show no staining of tissues known to lack the antigen" Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered "best practice"... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual." Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From jenoskins <@t> gmail.com Tue Apr 29 13:02:04 2014 From: jenoskins <@t> gmail.com (Jennifer Oskins) Date: Tue Apr 29 13:02:08 2014 Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! Message-ID: Histonetters- I am trying to help a co-worker find contact information for Seventh Wave Pathology. Does anyone have information for this Lab? Are there any other Histology/Pathology Labs that are recommended for processing, staining and evaluating tissue samples? Thank you so much in advance! Jennifer From lori.garcia <@t> medtronic.com Tue Apr 29 13:08:18 2014 From: lori.garcia <@t> medtronic.com (Garcia, Lori, M.Sc.) Date: Tue Apr 29 13:09:10 2014 Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! In-Reply-To: References: Message-ID: <8214022B32A9DE4985A37EA7AE814DA721EBD3@MSPM1BMSGM41.ent.core.medtronic.com> Here is contact person: Kim Sagartz President & Chief Operating Officer Senior Director, Client Services Seventh Wave Laboratories LLC 743 Spirit 40 Park Drive, Suite 209 Chesterfield MO 63005 Telephone: 636-519-4885 Facsimile: 636-519-4886 www.7thwavelabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Oskins Sent: Tuesday, April 29, 2014 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! Histonetters- I am trying to help a co-worker find contact information for Seventh Wave Pathology. Does anyone have information for this Lab? Are there any other Histology/Pathology Labs that are recommended for processing, staining and evaluating tissue samples? Thank you so much in advance! Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From doug.porter <@t> caplab.org Tue Apr 29 13:13:07 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Tue Apr 29 13:11:25 2014 Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! In-Reply-To: References: Message-ID: <004101cf63d6$aca9bcf0$05fd36d0$@caplab.org> Jennifer, You can send your samples to us. We'd be happy to evaluate them for you. My contact information is below. I guess I should ask what kind of samples you have first though...! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Oskins Sent: Tuesday, April 29, 2014 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! Histonetters- I am trying to help a co-worker find contact information for Seventh Wave Pathology. Does anyone have information for this Lab? Are there any other Histology/Pathology Labs that are recommended for processing, staining and evaluating tissue samples? Thank you so much in advance! Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4577 / Virus Database: 3931/7411 - Release Date: 04/28/14 From doug.porter <@t> caplab.org Tue Apr 29 13:21:04 2014 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Tue Apr 29 13:19:23 2014 Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! In-Reply-To: References: Message-ID: <004801cf63d7$c9192320$5b4b6960$@caplab.org> Jennifer, If your samples happen to be of a non-human nature, let me suggest the following lab: Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 portera@msu.edu www.humanpathology.msu.edu Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Oskins Sent: Tuesday, April 29, 2014 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! Histonetters- I am trying to help a co-worker find contact information for Seventh Wave Pathology. Does anyone have information for this Lab? Are there any other Histology/Pathology Labs that are recommended for processing, staining and evaluating tissue samples? Thank you so much in advance! Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4577 / Virus Database: 3931/7411 - Release Date: 04/28/14 From rgrow <@t> bmnet.com Tue Apr 29 13:27:01 2014 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Tue Apr 29 13:27:08 2014 Subject: [Histonet] Job Opening in East Tennessee Message-ID: Blount Memorial Hospital in Maryville, TN will have a histology technician position opening in May. One of our histotechnician's is retiring after a long career for some well deserved time to do as she wants. We are located in east Tennessee just minutes from the beautiful Smoky Mountains National Park and experience 4 wonderful seasons! Applicants must meet the educational and training requirements necessary for certification by the American Society of Clinical Pathology as a Histology Technician, HTL, or have experience equal to certification. Salary commiserate with experience. Please respond via email with resume attached to: RGROW@BMNET.COM . Thank you Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From mward <@t> wakehealth.edu Tue Apr 29 14:46:37 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Tue Apr 29 14:46:42 2014 Subject: [Histonet] CAP question ANP.22978 - Her2 assay validation In-Reply-To: References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> Message-ID: Hello all, I have been reading through the most recent revisions and want to see how others are handling this question. The explanation states that it is for new and existing assays and that if your validation does not meet current standards that you must supplement and bring it into compliance. Furthermore if you do not have any documentation from the initial validation the assay must be fully revalidated and documented. Our lab has been performing the Herceptest from Dako (FDA approved) since before 2008 and participating in the HER2 proficiency testing since it was first offered. We have our statistical results comparing our IHC patient results to FISH Her2 results since 2008 and we have always done well on our CAP proficiency testing (95%-100%). We do inter-pathologist result comparisons, using know CAP slides and have 95% to 100% agreements. What I do not have however is the original results of the slides that were stained to set up the original assay. Under these circumstances will we need to completely revalidate the assay, using the mandated 20+/20- cases, or can we simply do a retroactive formal review and write up of our past performances on our proficiency testing challenges? Thanks in advance for your help with this! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 From vperez <@t> pathreflab.com Tue Apr 29 16:18:26 2014 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Tue Apr 29 16:18:32 2014 Subject: [Histonet] RE: CAP question ANP.22978 - Her2 assay validation In-Reply-To: References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> Message-ID: >From what I have read and understand you should be able to do a write up the retroactive review based on the PT results. What we did here was bought a microarray slide that came with the HER2/ER/PR results, ran them on our machine, and compared our results to the ones that came with the slide. Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, April 29, 2014 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question ANP.22978 - Her2 assay validation Hello all, I have been reading through the most recent revisions and want to see how others are handling this question. The explanation states that it is for new and existing assays and that if your validation does not meet current standards that you must supplement and bring it into compliance. Furthermore if you do not have any documentation from the initial validation the assay must be fully revalidated and documented. Our lab has been performing the Herceptest from Dako (FDA approved) since before 2008 and participating in the HER2 proficiency testing since it was first offered. We have our statistical results comparing our IHC patient results to FISH Her2 results since 2008 and we have always done well on our CAP proficiency testing (95%-100%). We do inter-pathologist result comparisons, using know CAP slides and have 95% to 100% agreements. What I do not have however is the original results of the slides that were stained to set up the original assay. Under these circumstances will we need to completely revalidate the assay, using the mandated 20+/20- cases, or can we simply do a retroactive formal review and write up of our past performances on our proficiency testing challenges? Thanks in advance for your help with this! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Tue Apr 29 16:28:20 2014 From: suetp918 <@t> comcast.net (Sue) Date: Tue Apr 29 16:28:38 2014 Subject: [Histonet] Your experience with barcoding and materials tracking in grossing and histology In-Reply-To: <761E2B5697F795489C8710BCC72141FF3677D39A@ex07.net.ucsf.edu> Message-ID: <817715931.1523521.1398806900934.JavaMail.root@sz0028a.westchester.pa.mail.comcast.net> So we have the Vantage system with CoPath AB&T.? With CoPath you cannot use Vantage unless you get AB&T.? I actually like Vantage alot.? There are some issues that make it a little difficult to use and I would love to talk to you directly since trying to put it all in an e-mail is crazy.? If you want you can call my cell 856-905-1549. Sue Paturzo TJUH From deliacs <@t> uhnj.org Tue Apr 29 21:14:23 2014 From: deliacs <@t> uhnj.org (Delia, Catherine) Date: Tue Apr 29 21:14:30 2014 Subject: [Histonet] Question about high complexity testing for histotechnologists Message-ID: <97BC9AFB15C7A5488E52257DB913EE184D725A8346@UHEXMBX01.core.umdnj.edu> Does anyone know if there is a regulatory agency that requires high complexity testing certification for histotechnologists. Catherine Susan Delia, BS. HT. ASCP Chief Technologist, Anatomic Pathology University Hospital 150 Bergen Street E-151 Newark, New Jersey 07103 Phone: 973-972-5717 Cell: 908-391-1060 Fax: 973-972-5724 deliacs@uhnj.org From tmcampbell <@t> fmh.org Wed Apr 30 06:47:54 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Wed Apr 30 06:49:21 2014 Subject: [Histonet] Re: Negative Controls In-Reply-To: References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> Message-ID: <3566D9E34287BE4B95372179009446A01CFDB11F@EXCHANGE.fmhnt.fmh.org> So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: "must show no staining of tissues known to lack the antigen" Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered "best practice"... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual." Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbell <@t> fmh.org Wed Apr 30 06:49:46 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Wed Apr 30 06:51:07 2014 Subject: [Histonet] Re: Negative Controls In-Reply-To: References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> Message-ID: <3566D9E34287BE4B95372179009446A01CFDB129@EXCHANGE.fmhnt.fmh.org> As of now, my lab took that as you just needed one negative slide per run. And that negative slide is getting the negative mouse or rabbit serum. Is this correct? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: "must show no staining of tissues known to lack the antigen" Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered "best practice"... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual." Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbell <@t> fmh.org Wed Apr 30 06:53:27 2014 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Wed Apr 30 06:53:26 2014 Subject: [Histonet] PT Message-ID: <3566D9E34287BE4B95372179009446A01CFDB134@EXCHANGE.fmhnt.fmh.org> I need to enroll in a Proficiency testing program just for the technical part. I am looking for the cheapest program that CLIA would recognize. I am only CLIA certified. Thanks. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From LSebree <@t> uwhealth.org Wed Apr 30 07:34:36 2014 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Apr 30 07:34:42 2014 Subject: [Histonet] Re: Negative Controls In-Reply-To: <3566D9E34287BE4B95372179009446A01CFDB11F@EXCHANGE.fmhnt.fmh.org> References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> <3566D9E34287BE4B95372179009446A01CFDB11F@EXCHANGE.fmhnt.fmh.org> Message-ID: <77DD817201982748BC67D7960F2F76AF0B2CD1@UWHC-MBX12.uwhis.hosp.wisc.edu> Tasha, We use the negative elements within our patient sample as our "negative tissue control". Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: "must show no staining of tissues known to lack the antigen" Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered "best practice"... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual." Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Wed Apr 30 07:40:46 2014 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Wed Apr 30 07:40:55 2014 Subject: [Histonet] Re: Negative Controls In-Reply-To: <77DD817201982748BC67D7960F2F76AF0B2CD1@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> <3566D9E34287BE4B95372179009446A01CFDB11F@EXCHANGE.fmhnt.fmh.org> <77DD817201982748BC67D7960F2F76AF0B2CD1@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: So do we. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, April 30, 2014 8:35 AM To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls Tasha, We use the negative elements within our patient sample as our "negative tissue control". Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: "must show no staining of tissues known to lack the antigen" Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered "best practice"... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual." Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Wed Apr 30 07:50:51 2014 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Apr 30 07:51:06 2014 Subject: [Histonet] Re: Negative Controls In-Reply-To: <77DD817201982748BC67D7960F2F76AF0B2CD1@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> <3566D9E34287BE4B95372179009446A01CFDB11F@EXCHANGE.fmhnt.fmh.org> <77DD817201982748BC67D7960F2F76AF0B2CD1@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: So do we Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, April 30, 2014 8:35 AM To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls Tasha, We use the negative elements within our patient sample as our "negative tissue control". Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: "must show no staining of tissues known to lack the antigen" Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered "best practice"... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual." Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies "Appropriate negative controls are used." ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Stacy_McLaughlin <@t> cooley-dickinson.org Wed Apr 30 08:42:39 2014 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Wed Apr 30 08:42:45 2014 Subject: [Histonet] Special stain control validation- CAP compliance In-Reply-To: References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> <3566D9E34287BE4B95372179009446A01CFDB11F@EXCHANGE.fmhnt.fmh.org> <77DD817201982748BC67D7960F2F76AF0B2CD1@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Hello, We validate our control blocks prior to patient use. We label the slide with the stain, date, and results. We keep the slides in a file for reference. Our recent CAP inspection cited us on this because it was not documented on paper. I am considering challenging this, as I feel what we're doing meets the intent of the checklist question. What are your opinions? Thanks, Stacy **NEW** 07/29/2013 ANP.21460 Special Stain Controls Phase I Validated tissue controls are required for each special stain. NOTE: Positive tissue controls assess the performance of the special stain. Special stains are performed on sections of control tissue known to contain components specific to each special stain. Validation of tissue used as a positive control must be performed and documented before being used with clinical specimens. Evidence of Compliance: ? Written results of special stain control tissue validation [Cooley-Dickinson.org] From rjbuesa <@t> yahoo.com Wed Apr 30 09:07:10 2014 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Apr 30 09:07:15 2014 Subject: [Histonet] Question about high complexity testing for histotechnologists In-Reply-To: <97BC9AFB15C7A5488E52257DB913EE184D725A8346@UHEXMBX01.core.umdnj.edu> References: <97BC9AFB15C7A5488E52257DB913EE184D725A8346@UHEXMBX01.core.umdnj.edu> Message-ID: <1398866830.1071.YahooMailNeo@web120403.mail.ne1.yahoo.com> NO, because the license in "histotechnology" INCLUDES high complexity testing. On the other hand there are some tests like FISH with the VYSIS system that requires a special training with its own certification. Ren? J.? On Tuesday, April 29, 2014 10:15 PM, "Delia, Catherine" wrote: Does anyone know if there is a regulatory agency that requires high complexity testing certification for histotechnologists. Catherine Susan Delia, BS. HT. ASCP Chief Technologist, Anatomic Pathology University Hospital 150 Bergen Street E-151 Newark, New Jersey 07103 Phone: 973-972-5717 Cell: 908-391-1060 Fax: 973-972-5724 deliacs@uhnj.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vperez <@t> pathreflab.com Wed Apr 30 09:10:49 2014 From: vperez <@t> pathreflab.com (Vanessa Perez) Date: Wed Apr 30 09:11:00 2014 Subject: [Histonet] RE: CAP question ANP.22978 - Her2 assay validation In-Reply-To: References: <20140429161757.45B521E8083@trendmess-svr.holyredeemer.local> Message-ID: https://www.biomax.us/tissue-arrays/Breast/ theres the link, they have various kinds, so just choose the one that comes with the IHC results Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: Martha Ward-Pathology [mailto:mward@wakehealth.edu] Sent: Wednesday, April 30, 2014 7:11 AM To: Vanessa Perez Subject: RE: CAP question ANP.22978 - Her2 assay validation Thanks. I like the idea of the microarray slide as well. Where did you purchase your slide? martha -----Original Message----- From: Vanessa Perez [mailto:vperez@pathreflab.com] Sent: Tuesday, April 29, 2014 5:18 PM To: Martha Ward-Pathology; histonet@lists.utsouthwestern.edu Subject: RE: CAP question ANP.22978 - Her2 assay validation >From what I have read and understand you should be able to do a write up the retroactive review based on the PT results. What we did here was bought a microarray slide that came with the HER2/ER/PR results, ran them on our machine, and compared our results to the ones that came with the slide. Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vperez@pathreflab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, April 29, 2014 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question ANP.22978 - Her2 assay validation Hello all, I have been reading through the most recent revisions and want to see how others are handling this question. The explanation states that it is for new and existing assays and that if your validation does not meet current standards that you must supplement and bring it into compliance. Furthermore if you do not have any documentation from the initial validation the assay must be fully revalidated and documented. Our lab has been performing the Herceptest from Dako (FDA approved) since before 2008 and participating in the HER2 proficiency testing since it was first offered. We have our statistical results comparing our IHC patient results to FISH Her2 results since 2008 and we have always done well on our CAP proficiency testing (95%-100%). We do inter-pathologist result comparisons, using know CAP slides and have 95% to 100% agreements. What I do not have however is the original results of the slides that were stained to set up the original assay. Under these circumstances will we need to completely revalidate the assay, using the mandated 20+/20- cases, or can we simply do a retroactive formal review and write up of our past performances on our proficiency testing challenges? Thanks in advance for your help with this! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jenoskins <@t> gmail.com Wed Apr 30 09:21:31 2014 From: jenoskins <@t> gmail.com (Jennifer Oskins) Date: Wed Apr 30 09:25:06 2014 Subject: [Histonet] Thank You! Message-ID: All- A very sincere thank you to everyone who has responded so graciously to my request for information for Seventh Wave Pathology and for additional suggestions for CRO Histology/Pathology services. I have forwarded the information on. Thank you again for the great feedback! Jennifer From CDavis <@t> che-east.org Wed Apr 30 10:10:29 2014 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Wed Apr 30 10:09:53 2014 Subject: [Histonet] animal & human biopsies Message-ID: Some threw the thought of processing biopsies from veterinary clinics as a way of increasing outpatient volumes. I am concerned there may be a regulation against this. Does anybody know of any? Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From fpearsa <@t> clemson.edu Wed Apr 30 11:53:31 2014 From: fpearsa <@t> clemson.edu (Frances Pearsall) Date: Wed Apr 30 11:53:37 2014 Subject: [Histonet] Formalin fixation problems Message-ID: <6B70E8BA65CBCC45B5818604BC42F4D0249D0973@EXCH10MBX01.CAMPUS.CU.CLEMSON.EDU> Recently we started having problems with our 10% nb formalin not completely fixing our animal tissues. We haven't done any thing different in making up the solutions, but we're finding incomplete fixation in the center of the tissues. We are letting it stay in formalin the same amount of time (24-48 hrs) as we've always done. The pH has not changed. Does any one have any suggestions as to where we can look to trouble shoot correcting this problem? Our recipe method is: 4g Sodium Phosphate, monobasic monohydrate(Fisher Chemical co.) 6.5g Sodium Phosphate, dibasic anhydrous(EMD Chemical co.) 37% Formaldehyde(EMD Chem.co.) 10 parts formaldehyde to 90 parts Dh20. Any helpful thoughts would be appreciated. Thank you, Fran Pearsall HT From patrick.lewis <@t> seattlechildrens.org Wed Apr 30 12:43:10 2014 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Apr 30 12:43:38 2014 Subject: [Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at? Message-ID: <3903BE18914F4440834F0E620415FFCA3828C45D@PPWEXD01a.childrens.sea.kids> Hi everyone, What is a good way to post pictures of slides for you guys to look at? I had a weird black precipitate in one of my IHCs and I'd like for you guys to look at them and see if you recognize what this artifact is? Thanks Patrick. PS: I tried uploading them as attachments but the size was too large even though I tried to crop things to keep the size to a minimum. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From POWELL_SA <@t> mercer.edu Wed Apr 30 12:57:10 2014 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Apr 30 12:57:18 2014 Subject: [Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at? In-Reply-To: <3903BE18914F4440834F0E620415FFCA3828C45D@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA3828C45D@PPWEXD01a.childrens.sea.kids> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF76AD20E@MERCERMAIL.MercerU.local> HI Patrick, Marvin Hanna posted these instructions in the below message on histonet yesterday. This is a good way to post images you wish to discuss on histonet. He has made available a way to do this for the histonet community, as well as has the archives of histonet on the www.histosearch.com website. You cannot post images on histonet. Shirley Powell Hi Histonetters, > > Tim Morken asked me about posting images for discussion on Histonet a couple of weeks ago and we noticed our images website is no longer running. As we move into digital pathology, having the ability to post images to discuss on Histonet is important. So, we set up an easy way to post images for Histonet on Histosearch at http://histosearch.com/imageupload/. You need to fill in your name, a title for your images, select images to upload from your computer or smartphone and click submit. There is no need to register and it takes about 2 minutes. We intentionally did not include comments on the website so the discussions will take place on Histonet. > > We have also set up mobile pages for searching Histosearch and the archives at http://m.histosearch.com and http://m.histosearch.com/histonet/ for histologists on the go. You may also want to try out the new image searching capabilities on Histosearch. After searching with keyword(s) such as ki67 or alcian blue, there is a link at the top of the results page for "images". > Clicking this will provide images related to your keyword(s) from the over 12 million histopathology related pages searched in Histosearch. We believe we have the largest list of histology educational resources on the internet at Histosearch and they are searchable for text and images. > Let us know if we've missed any histology resources. > > Best Regards, > > Marvin Hanna > -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Wednesday, April 30, 2014 1:43 PM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at? Hi everyone, What is a good way to post pictures of slides for you guys to look at? I had a weird black precipitate in one of my IHCs and I'd like for you guys to look at them and see if you recognize what this artifact is? Thanks Patrick. PS: I tried uploading them as attachments but the size was too large even though I tried to crop things to keep the size to a minimum. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhanna <@t> histosearch.com Wed Apr 30 13:04:26 2014 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Wed Apr 30 13:04:34 2014 Subject: [Histonet] Is there a way to post pictures of slides on histonet? Or to make them available for people to look at? In-Reply-To: <3903BE18914F4440834F0E620415FFCA3828C45D@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA3828C45D@PPWEXD01a.childrens.sea.kids> Message-ID: <53613B2A.8090506@histosearch.com> Hi Patrick, You can upload images for Histonet at http://histosearch.com/imageupload/. The Histonet listserver does not allow attachments to messages to prevent the spread of malware. Let me know if you have any problems. Best Regards, Marvin Hanna On 04/30/2014 01:43 PM, Lewis, Patrick wrote: > Hi everyone, > > What is a good way to post pictures of slides for you guys to look at? > > I had a weird black precipitate in one of my IHCs and I'd like for you guys to look at them and see if you recognize what this artifact is? > > Thanks > > Patrick. > > PS: I tried uploading them as attachments but the size was too large even though I tried to crop things to keep the size to a minimum. > > Thanks > > Patrick. > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patrick.lewis <@t> seattlechildrens.org Wed Apr 30 13:05:20 2014 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Apr 30 13:05:29 2014 Subject: [Histonet] I just posted 2 photos to histosearch Message-ID: <3903BE18914F4440834F0E620415FFCA3828D48C@PPWEXD01a.childrens.sea.kids> Thanks everyone, I just posted 2 images to histo search. IS there a way to post up to 5 pics at once? It looked like it suggested that you could post up to 5 pics as a group but I didn't see HOW to do it. Anyway, I have one post of outside of the tissue area, and one post of the tissue area. What is this weird black precipitate? I was using Red AEC as a substrate. Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From liz <@t> premierlab.com Wed Apr 30 13:20:38 2014 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Apr 30 13:20:43 2014 Subject: [Histonet] Awards Spotlight - The Newcomer Helping Hand Award Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E05A09@SBS2K8.premierlab.local> Award Spotlight - The Newcomer Helping Hand Award This award is sponsored by Newcomer Supply Inc. and consist of a $1500.00 grant. This is a new award for the NSH it has only been given out once so far, last year was the first year it was given and Jean Mitchell NSH's Region IV director was its first recipient. Jean knows first-hand what it's like to help others internationally she was able to travel to Botswana, Africa in August 2013 as part of an ASCP Laboratory Mission to assess and teach good histotechnology practices in Botswana. This grant is intended to: Support national and or international histology education outreach efforts. Increase the quality and expertise of histology educational opportunities for student groups with limited or deteriorated access to educational facilities and educators. Affect efforts to recruit future generations of histotechnologists and promote the study of histology. Provide needed educational supplies or equipment. This grant can be used to support any educational opportunity such as hosting high school students in your laboratory or at a STEM event. Nominate yourself if you are planning such an event or a colleague if you know that they have been involved in educational opportunities in the past and are planning to do so in the future at the following link http://nsh.org/scholarships-awards/advocacy/service%20awards?nid=2930 This is just one way that this award can be used. Helping others both nationally and internationally learn about histology can be a really fun experience. We host students yearly in our lab and it is an exhausting but very rewarding experience. Getting our young students interested in science is in my opinion is a really worthy cause especially if I can do that by teaching them about histology. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From mhanna <@t> histosearch.com Wed Apr 30 17:13:23 2014 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Wed Apr 30 17:13:31 2014 Subject: [Histonet] I just posted 2 photos to histosearch In-Reply-To: <3903BE18914F4440834F0E620415FFCA3828D48C@PPWEXD01a.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA3828D48C@PPWEXD01a.childrens.sea.kids> Message-ID: <53617583.8010105@histosearch.com> Hi Patrick, To post more than one image, there is a link under "choose file" where you uploaded the first picture for "add another image". When you click "add another image", it opens up another "choose file" box for a second image to upload. Or you can just post them one at a time if you like. I've seen that black precipitate on slides in an IHC lab I was at a couple of years ago. If I remember correctly, we thought it was a formalin-heme pigment which can be caused when formalin is acidic (not buffered). Marvin On 04/30/2014 02:05 PM, Lewis, Patrick wrote: > Thanks everyone, > > I just posted 2 images to histo search. IS there a way to post up to 5 pics at once? It looked like it suggested that you could post up to 5 pics as a group but I didn't see HOW to do it. > > Anyway, I have one post of outside of the tissue area, and one post of the tissue area. > > What is this weird black precipitate? I was using Red AEC as a substrate. > > Patrick. > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lins0701 <@t> gmail.com Wed Apr 30 20:47:16 2014 From: lins0701 <@t> gmail.com (Sophia Lin) Date: Wed Apr 30 20:47:21 2014 Subject: [Histonet] gmed/ggastro interfacing with pathology for seamless transition? Message-ID: Hi all, Our practice is moving forward to bringing in-house pathology since we already have in-house histology. Any gastro practices that use gmed/ggastro and have a seamless pathology module that can be added for a smooth transition? This would entail using ggastro/gmed and interfacing with the pathology module. We currently use WINSURGE but cannot interface with ggmed/gastro easily. Any thoughts? Thanks, Sophia