[Histonet] edge effect on IHC

[Tim Higgins]

Hey Mary,



I would suggest observing your
stainer to ensure the proper drop zones are being performed and not just
dropping closest to the label. I used Dako for years and loved them but you do
have to make sure it dispenses properly from time to time. They use drop zones
unlike Ventana which uses a vortex type of mixing.



Good luck!



Thanks,



Timothy N. Higgins, HT (ASCP), QIHC



 

> Date: Mon, 23 Sep 2013 14:00:57 -0700 (PDT)
> From: Mary Benoit <mbplab <@t> yahoo.com>
> Subject: [Histonet] edge effect on IHC
> To: "histonet <@t> lists.utsouthwestern.edu"
> 	<histonet <@t> lists.utsouthwestern.edu>
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> I have been aware of some "edge effect" on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma met to colon that was well fixed and processed that stained only the edge, however when we sent block to Neogenomics reference lab, their slide stained evenly over the entire tumor area. We both used a melanoma cocktail. Not sure what is going on. Any suggestions?
> Thank you
> Mary F Benoit, MT(ASCP)
> The Pathology Laboratory
> 830 Bayou Pines Drive
> Lake Charles, LA 70601
> mbplab <@t> yahoo.com