[Histonet] Re: bunsen burner at the embedding center
Blazek, Linda
lblazek <@t> digestivespecialists.com
Fri Sep 20 13:14:42 CDT 2013
Lots of us and we survived!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Tournear
Sent: Friday, September 20, 2013 1:55 PM
To: Paula Sicurello
Cc: Histonet <@t> lists.utsouthwestern.edu; Bob Richmond
Subject: Re: [Histonet] Re: bunsen burner at the embedding center
How many of us smoked and drank coffee at our microtomes? Or while doing autopsies? Those were the days and a lot of us are still around. Lol
Sent from the iPhone of Kim Tournear 🇺🇸 😃
On Sep 20, 2013, at 8:44 AM, Paula Sicurello <patpxs <@t> gmail.com> wrote:
> I worked for the Navy Hospital in San Diego, way back in the day, and
> we did an extraction with fuming HCl (straight, 100% HCl for folks who
> didn't know what fuming meant). Several people would get nosebleeds
> every time they performed the extraction. I finally got one of those
> fans that looks like an airplane propellor in a cage and pried open
> the painted shut windows. I dared anyone to stop me, no one did! 1
> was quite gutsy for a
> 22 year old.
> The Petty Officers had me make 10N NaOH and forgot to tell me how hot
> it got. I almost dropped it when I picked it up.
>
> Plus I had to swim to work, both ways in a rip tide-battleing sharks
> and jelly fish, while sloshing around in an earthquake! It is San
> Diego after all.
>
> Happy Friday!
>
> Paula
> --
> Paula Sicurello, HTL (ASCP)
> Supervisor, Clinical Electron Microscopy Laboratory Duke University
> Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina
> 27710
> P: 919.684.2091
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> On Fri, Sep 20, 2013 at 11:25 AM, jeff lowen <lowenjeff <@t> hotmail.com> wrote:
>
>> yet here we are......how much of what they tell us is hyperbole?
>>
>>> Date: Fri, 20 Sep 2013 14:50:24 +0000
>>> From: mucram11 <@t> comcast.net
>>> To: rsrichmond <@t> gmail.com
>>> Subject: Re: [Histonet] Re: bunsen burner at the embedding center
>>> CC: histonet <@t> lists.utsouthwestern.edu
>>>
>>>
>>>
>>> I actually knew the person you are speaking of and it was his
>>> favorite
>> trick. We should be careful these younger people are using gloves
>> for everything now to protect themselves. W hen I started in
>> Histology even the pathologists cleaned the paraffin off their hands
>> with xylene and encouraged everyone to do the same . We used so many
>> things that are now not even allowed to be open on this planet and
>> did know how dangerous or serious the possible issues could be over time.
>>>
>>>
>>> Pam Marcum
>>>
>>>
>>>
>>> ----- Original Message -----
>>> From: "Bob Richmond" <rsrichmond <@t> gmail.com>
>>> To: "Histonet <@t> lists.utsouthwestern.edu" <
>> histonet <@t> lists.utsouthwestern.edu>
>>> Sent: Friday, September 20, 2013 9:35:55 AM
>>> Subject: [Histonet] Re: bunsen burner at the embedding center
>>>
>>> I distinctly remember when I was a resident at Johns Hopkins in the
>>> late 1960s that the histotechs would smoke while staining and
>>> coverslipping without much ventilation. When I suggested to the
>>> chief technologist (who later died of smoking related disease at 65,
>>> but at least he hadn't set himself afire) that this wasn't such a
>>> good idea, he responded by
>> stubbing
>>> out a lighted cigarette in a Stender dish full of xylene (apparently
>>> you can do this trick with gasoline also, but don't try it at home please).
>>>
>>> Buffering formalin was prohibited back then, and they removed the
>> formalin
>>> pigment by passing the sections through a concentrated solution (20
>>> or
>> 30%)
>>> of picric acid in acetone. I'm glad he didn't try the cigarette
>>> trick in THAT Stender dish.
>>>
>>> Fast-forward nearly half a century, and in the three labs I'm
>>> working in I'm still grossing formalin-fixed tissue with minimal
>>> ventilation, but at least people aren't allowed to smoke in the lab any more.
>>>
>>> Bob Richmond
>>> Samurai Pathologist
>>> Maryville TN
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>>
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