[Histonet] section thickness for Picro sirius red stain

[Grantham, Andrea L - (algranth)]

I've been doing this PSR stain for a few years now on various types of tissue sections of pretty much routine thickness (4-5 microns). I was asked today if the stain would penetrate a thicker section - like 40 or more microns. Even a vibratome section.
The tissue is pig optic nerve and a small bit of surrounding tissue.
When stained with PSR, the routine thickness sections were awesome.



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097