[Histonet] Bringing new antibodies in house - Ki-67,P40,PAX8

[Natalie Nagy]

Hello Everyone,
                      I am currently looking to bring a few new antibodies in house; KI-67,P40, and PAX8. I have not worked with these antibodies yet and wanted to get some opinions from everyone. For P40 and PAX8; are you using a monoclonal or polyclonal? Also what company are you getting them from? My hospital deals mainly with Dako, Biocare, and Cellmarque.  We are a very small facility that does HIER with a waterbath, and then manual IHC pipetting with the old sequenza coverplate tiles. So usually we prefer concentrate for antibodies not pre-dilute because of the longer expiration times, and of course the cost. So I guess what I am asking is, what clone do you prefer. What company do you get it from? How much does it cost? And have you ever run it on a manual setup, if so how was it to work with?
 
Thanks for all the help in advance,
 
Natalie J. Nagy
Histology Supervisor
Holyoke Medical Center
 
nagy_natalie <@t> holyokehealth.com


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