[Histonet] longterm storage of tissue samples in 70% Ethanol

[Caroline Miller]

Sometimes in the research field or is necessary to store up samples without processing, either waiting for other samples from the set, or the cost to the researcher of processing tissue that they might not need

We always advise our users to fix adequately and then store in 70% until they process. We have never had a problem with this, even when samples have been stored like this for months

The theory is that there is just enough alcohol to prevent dehydration or rehydration and it is in 'steady state'

Any concrete evidence to this fact would be greatly welcomed though, I am going on completely empirical evidence!

Yours
Caroline

Caroline Miller
Gladstone Institutes
www.gladstoneinstitutes.org

Tel: 415 7342566
Cell: 415 2187297




On Sep 18, 2013, at 7:21 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> Why do you have to store fixed specimens for that long? If you are going to process them anyway, why don't you just process them and store as paraffin blocks? It will be much safer and you can be sure of the antigens preservation.
> René J.
> 
> 
> ________________________________
> From: "Kruttwig, Klaus" <Klaus.Kruttwig <@t> ucsf.edu>
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
> Sent: Tuesday, September 17, 2013 6:52 PM
> Subject: [Histonet] longterm storage of tissue samples in 70% Ethanol
> 
> 
> Dear all,
> 
> I have a question about longterm storage of histology samples and was wondering if someone can help me.
> I am not sure if my current sample storage system is optimal in terms of antigen- and histology preservation. Do you think it is okay to store 4% paraformaldehyde fixated samples before paraffin embedding for approx 2-3 years in 70% EtOH at 4 degree celsius? Or do I risk a decrease in specimen quality?
> 
> Any help will be highly appreciated.
> 
> Thanks,
> Klaus
> 
> Klaus Kruttwig
> Department of Cell and Tissue Biology
> University of California - San Francisco
> San Franscisco, CA, USA
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