[Histonet] ZINC formalin vs NBF for IHC

[Paula Pierce]

CD34 and CD31 do work better with a zinc formalin fixation, but you still need AR.

The zinc will cause a precipitate from the phosphates. A few specimens will not create enough to be a problem. If a large number are needed, rinse the tissues first in PBS or 70%EtOH or process on the last run before changing the processor.
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com


________________________________
 From: "Fischer, Dolores" <Dolores_Fischer <@t> baxter.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, September 18, 2013 8:27 AM
Subject: [Histonet] ZINC formalin vs NBF for IHC
 

Good morning everyone!

I would like opinions on the use of zinc formalin for IHC.  Pros and cons??  A new pathologist would like us to use zinc formalin for working out a protocol for CD34 and/or CD31 in mouse.   I have always worked up needed protocols in 10% NBF with no problems and feel using zinc formalin will only throw in a variable that we don't need to add.  I also feel that NBF is the fixative of choice for most antibodies and procedures.  (small survey what do you use?)  Am I not being open minded enough? What zinc formalin is recommended for use in a VIP processor and for IHC?   I have read that there may be precipitation problems with this fixative.  Opinions are appreciated, I did try to search the archives but didn't find too much information on this subject.  I am trying to build a case for NOT using zinc formalin.  I believe our pathologist is trying to save us the antigen retrieval step in IHC.  Opinions??

Thanks all,

Dolores

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