[Histonet] Processing Problem

[Sandra Cheasty]

Hi All,

                I need help with two issues. I have a researcher who is sending us porcine skin, about 2mm x 2mm x 1mm tall. 

 

Issue 1: There is a lesion in the center, and although he wants the skin sectioned through the lesion eventually, he says if he bisects the chunk of skin before fixation, the lesion becomes distorted. So, he is fixing them in the 2x2x1 chunks, and the 10% formalin (and subsequent processing reagents) are not penetrating. Does anyone know of either a more penetrating fixative or a less distorting one so we can bisect the skin before fixation?

 

Issue 2: Even on smaller sections that fixed and processed well, we are having issues with the porcine skin sections staying on the slides. We use Superfrost Plus slides, drain them, air dry them overnight, and then they go on the "Extra Oven" program on the stainer. (25 minutes in the oven.) Any suggestions on other slides, drying techniques to try?

 

My background is that of a certified Histologist for 30 years, with experience in many labs in various parts of the country. This research project has me stymied!

 

Thanks for your help!

Sandy

UW Madison 

School of Veterinary Medicine