[Histonet] Re: Histonet Digest, Vol 118, Issue 24
Madeleine Huey
madeleinehuey <@t> gmail.com
Thu Sep 12 12:18:02 CDT 2013
From:"Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, September 11, 2013 4:46 PM
Subject: [Histonet] Equipment selection
Hi all,
I am trying to decide on what equipment to purchase. I have picked the
processor, microtomes, embedding centers and a few other pieces, but now I
still need to decide on the H&E Stainer and the immunostainer. My options I
have in front of me are for the H&E stainer are Sakura Prisma stainer/film
coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
Max. Any advice on any of these instruments would be greatly appreciated. I
am trying to finalize my decision pretty quickly. Thank you in advance for
any input.
Haley,
I have experiences with all the above equipments and here's my inf;
1) Leica H&E autostainer with glass coverslipper - smaller size (use lesser
counter space)
cheaper (buy 2 equivalent to 2 Sakura Prisma + film coverslipper and
smaller.
On Thu, Sep 12, 2013 at 10:02 AM, <histonet-request <@t> lists.utsouthwestern.edu
> wrote:
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> Today's Topics:
>
> 1. RE: Equipment selection (Huggins, Haley - MRMC)
> 2. Re: Equipment selection (Rene J Buesa)
> 3. RE: Equipment selection (Huggins, Haley - MRMC)
> 4. RE: Equipment selection (Marcum, Pamela A)
> 5. Need your expertise (Webb, Dorothy L)
> 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 12 Sep 2013 08:55:39 -0700
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> Subject: RE: [Histonet] Equipment selection
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4F36EC93A5737D4F8A2974E8FB8E260615F5698C69 <@t> PHX-MSG-007-N2.chw.edu
> >
> Content-Type: text/plain; charset="iso-8859-1"
>
> I actually know all the equipment. Well, I know 3 of the pieces really
> well, the Leica Bond Max is new to me. I did go to another lab to see it as
> well as the Leica stainer/coverslipper. I was impressed with the Bond Max,
> not as much with the coverslipper part of the stainer/coverslipper. The big
> issue I am having is if I want to go with the Bond Max, I technically need
> to go with the Leica stainer/coverslipper because it is lumped into a big
> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go
> with the Dako immunostainer. I am having trouble deciding. It is boiling
> down to money, which sucks since I might be forced to go with something
> that I might not be happy with in the future.
>
> Haley
>
> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 5:32 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> I used the Sakura and the Dako, but it seems that the Leica Bond Max is
> getting popularity. Why don't you go to a lab where they are in use and
> take a look?
> René J.
>
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org<mailto:
> Haley.Huggins <@t> DignityHealth.org>>
> To: "histonet <@t> lists.utsouthwestern.edu<mailto:
> histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu
> <mailto:histonet <@t> lists.utsouthwestern.edu>>
> Sent: Wednesday, September 11, 2013 4:46 PM
> Subject: [Histonet] Equipment selection
>
> Hi all,
>
> I am trying to decide on what equipment to purchase. I have picked the
> processor, microtomes, embedding centers and a few other pieces, but now I
> still need to decide on the H&E Stainer and the immunostainer. My options I
> have in front of me are for the H&E stainer are Sakura Prisma stainer/film
> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
> Max. Any advice on any of these instruments would be greatly appreciated. I
> am trying to finalize my decision pretty quickly. Thank you in advance for
> any input.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu
> >
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] Equipment selection
> To: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1379002076.53612.YahooMailNeo <@t> web163105.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> If buying the Bond means that you are forced to "swallow" the Leica
> coverslipper go with Sakura and Dako.
> If they are making that "package" is because the Bond sells well but they
> are having to "swallow" the coverslipper. Price is important but in the
> long run it is better to increase productivity and avoid problems. The
> Sakura is much faster than the Leica coverslipper.
> René J.
>
>
> ________________________________
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>; "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, September 12, 2013 11:55 AM
> Subject: RE: [Histonet] Equipment selection
>
>
>
> I actually know all the equipment. Well, I know 3 of the pieces really
> well, the Leica Bond Max is new to me. I did go to another lab to see it as
> well as the Leica stainer/coverslipper. I was impressed with the Bond Max,
> not as much with the coverslipper part of the stainer/coverslipper. The big
> issue I am having is if I want to go with the Bond Max, I technically need
> to go with the Leica stainer/coverslipper because it is lumped into a big
> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go
> with the Dako immunostainer. I am having trouble deciding. It is boiling
> down to money, which sucks since I might be forced to go with something
> that I might not be happy with in the future.
>
> Haley
>
> From:Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 5:32 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> I used the Sakura and the Dako, but it seems that the Leica Bond Max is
> getting popularity. Why don't you go to a lab where they are in use and
> take a look?
> RenéJ.
>
> From:"Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu
> >
> Sent: Wednesday, September 11, 2013 4:46 PM
> Subject: [Histonet] Equipment selection
>
> Hi all,
>
> I am trying to decide on what equipment to purchase. I have picked the
> processor, microtomes, embedding centers and a few other pieces, but now I
> still need to decide on the H&E Stainer and the immunostainer. My options I
> have in front of me are for the H&E stainer are Sakura Prisma stainer/film
> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
> Max. Any advice on any of these instruments would be greatly appreciated. I
> am trying to finalize my decision pretty quickly. Thank you in advance for
> any input.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 3
> Date: Thu, 12 Sep 2013 09:10:47 -0700
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> Subject: RE: [Histonet] Equipment selection
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D <@t> PHX-MSG-007-N2.chw.edu
> >
> Content-Type: text/plain; charset="iso-8859-1"
>
> Thanks, that is a good point.
>
> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 9:08 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> If buying the Bond means that you are forced to "swallow" the Leica
> coverslipper go with Sakura and Dako.
> If they are making that "package" is because the Bond sells well but they
> are having to "swallow" the coverslipper. Price is important but in the
> long run it is better to increase productivity and avoid problems. The
> Sakura is much faster than the Leica coverslipper.
> René J.
>
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org<mailto:
> Haley.Huggins <@t> DignityHealth.org>>
> To: Rene J Buesa <rjbuesa <@t> yahoo.com<mailto:rjbuesa <@t> yahoo.com>>; "
> histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>"
> <histonet <@t> lists.utsouthwestern.edu<mailto:
> histonet <@t> lists.utsouthwestern.edu>>
> Sent: Thursday, September 12, 2013 11:55 AM
> Subject: RE: [Histonet] Equipment selection
>
> I actually know all the equipment. Well, I know 3 of the pieces really
> well, the Leica Bond Max is new to me. I did go to another lab to see it as
> well as the Leica stainer/coverslipper. I was impressed with the Bond Max,
> not as much with the coverslipper part of the stainer/coverslipper. The big
> issue I am having is if I want to go with the Bond Max, I technically need
> to go with the Leica stainer/coverslipper because it is lumped into a big
> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go
> with the Dako immunostainer. I am having trouble deciding. It is boiling
> down to money, which sucks since I might be forced to go with something
> that I might not be happy with in the future.
>
> Haley
>
> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 5:32 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu<mailto:
> histonet <@t> lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Equipment selection
>
> I used the Sakura and the Dako, but it seems that the Leica Bond Max is
> getting popularity. Why don't you go to a lab where they are in use and
> take a look?
> René J.
>
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org<mailto:
> Haley.Huggins <@t> DignityHealth.org>>
> To: "histonet <@t> lists.utsouthwestern.edu<mailto:
> histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu
> <mailto:histonet <@t> lists.utsouthwestern.edu>>
> Sent: Wednesday, September 11, 2013 4:46 PM
> Subject: [Histonet] Equipment selection
>
> Hi all,
>
> I am trying to decide on what equipment to purchase. I have picked the
> processor, microtomes, embedding centers and a few other pieces, but now I
> still need to decide on the H&E Stainer and the immunostainer. My options I
> have in front of me are for the H&E stainer are Sakura Prisma stainer/film
> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
> Max. Any advice on any of these instruments would be greatly appreciated. I
> am trying to finalize my decision pretty quickly. Thank you in advance for
> any input.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu
> >
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 12 Sep 2013 16:30:13 +0000
> From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
> Subject: RE: [Histonet] Equipment selection
> To: "'Rene J Buesa'" <rjbuesa <@t> yahoo.com>, "Huggins, Haley - MRMC"
> <Haley.Huggins <@t> DignityHealth.org>, "
> histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57 <@t> Mail2Node2.ad.uams.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Actually the coverslipper from Leica is very good and can be linked to the
> stainer. It is also a glass coverslipper not tape. It is always about
> money and that may be the thing that decides no matter what any of prefers.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Thursday, September 12, 2013 11:08 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> If buying the Bond means that you are forced to "swallow" the Leica
> coverslipper go with Sakura and Dako.
> If they are making that "package" is because the Bond sells well but they
> are having to "swallow" the coverslipper. Price is important but in the
> long run it is better to increase productivity and avoid problems. The
> Sakura is much faster than the Leica coverslipper.
> René J.
>
>
> ________________________________
> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> To: Rene J Buesa <rjbuesa <@t> yahoo.com>; "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, September 12, 2013 11:55 AM
> Subject: RE: [Histonet] Equipment selection
>
>
>
> I actually know all the equipment. Well, I know 3 of the pieces really
> well, the Leica Bond Max is new to me. I did go to another lab to see it as
> well as the Leica stainer/coverslipper. I was impressed with the Bond Max,
> not as much with the coverslipper part of the stainer/coverslipper. The big
> issue I am having is if I want to go with the Bond Max, I technically need
> to go with the Leica stainer/coverslipper because it is lumped into a big
> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go
> with the Dako immunostainer. I am having trouble deciding. It is boiling
> down to money, which sucks since I might be forced to go with something
> that I might not be happy with in the future.
>
> Haley
>
> From:Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
> Sent: Thursday, September 12, 2013 5:32 AM
> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Equipment selection
>
> I used the Sakura and the Dako, but it seems that the Leica Bond Max is
> getting popularity. Why don't you go to a lab where they are in use and
> take a look?
> RenéJ.
>
> From:"Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu
> >
> Sent: Wednesday, September 11, 2013 4:46 PM
> Subject: [Histonet] Equipment selection
>
> Hi all,
>
> I am trying to decide on what equipment to purchase. I have picked the
> processor, microtomes, embedding centers and a few other pieces, but now I
> still need to decide on the H&E Stainer and the immunostainer. My options I
> have in front of me are for the H&E stainer are Sakura Prisma stainer/film
> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
> Max. Any advice on any of these instruments would be greatly appreciated. I
> am trying to finalize my decision pretty quickly. Thank you in advance for
> any input.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ----------------------------------------------------------------------
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>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 12 Sep 2013 11:45:41 -0500
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Subject: [Histonet] Need your expertise
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> 65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823 <@t> HPEMX3.HealthPartners.int>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We will finally be going green by recycling formalin. I would appreciate
> any advice or opinions on true recycling VS. a filtering type of system. As
> we all know, experience with a system or product is the best way to gain
> true knowledge!
>
> Also, I would appreciate knowing how others are handling extra levels of
> prostate needle bx's. We currently place them on charged slides and keep
> until the case is signed out, but it seems there should be a better way of
> handling this due to storage of the slides not to mention the extra cost
> involved! I know this is becoming more of a needed process with all of the
> RNA/DNA testing being done on diagnosed cancers.
>
> As always, I do appreciate any and all comments, advice, or words of
> wisdom!! Hope to see some of you in Providence late next week!
>
> Dorothy Webb, HT (ASCP)
> Regions Histology Technical Specialist
> 651-254-2962
>
>
>
> ________________________________
> This e-mail and any files transmitted with it are confidential and are
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>
>
> ------------------------------
>
> Message: 6
> Date: Thu, 12 Sep 2013 11:55:43 -0500
> From: Tim Higgins <thigginsht <@t> msn.com>
> Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY179-W92030A0D22235B31AF8D97D83A0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hey Martha,
>
> You have to forgive Rene J. she speaks sometimes and has nothing good to
> say but keeps speaking. There is never a bad question, if you don't have a
> good answer then keep it to yourself as I usually do.
>
> "Adequate forum". really? I guess no one on Histonet is allowed to share
> their personal protocols, procedures, opinions, and experiences on here
> anymore.
>
> Come on Rene J., next time shhhhhhhhh!!
>
> I should have just hit the "Delete" button and follow my own advise!
>
> Tim N. Higgins, HT (ASCP), QIHC
>
>
> > Message: 7
> > Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT)
> > From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> > Subject: Re: [Histonet] Minimum sample size for breast markers
> > To: Martha Ward-Pathology <mward <@t> wakehealth.edu>,
> > "histonet <@t> lists.utsouthwestern.edu"
> > <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> > <1378992154.20733.YahooMailNeo <@t> web163104.mail.bf1.yahoo.com>
> > Content-Type: text/plain; charset=iso-8859-1
> >
> > I do not think this is the adequate forum to ask this question because
> you may get a wrong answer that could determine your decision.
> > Your pathologist is the one who should decide about this issue.
> > René J.
> >
> >
> > ________________________________
> > From: Martha Ward-Pathology <mward <@t> wakehealth.edu>
> > To: "histonet <@t> lists.utsouthwestern.edu" <
> histonet <@t> lists.utsouthwestern.edu>
> > Sent: Thursday, September 12, 2013 9:06 AM
> > Subject: [Histonet] Minimum sample size for breast markers
> >
> >
> >
> >
> > Is there a minimum number of cells that should be present when testing
> for ER, PR and Her2? I have looked on the CAP website but cannot see
> anything about this. We are constantly getting these small biopsies or
> FNAs for testing and I sometimes wonder what the lowest threshold is for
> testing...20 tumor cells, 50 tumor cells???? Any information would be
> appreciated. Thanks in advance for your help.
> >
> > Martha Ward, MT (ASCP) QIHC
> > Manager
> >
> > Molecular Diagnostics Lab
> > Medical Center Boulevard \ Winston-Salem, NC 27157
> > p 336.716.2109 \ f 336.716.5890
> > mward <@t> wakehealth.edu
> >
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 118, Issue 24
> *****************************************
>
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