[Histonet] Oil red O

[Rene J Buesa]

If that is the case, ORO will stain fat droplets in Frozen Sections. In FFPE will stain nothing.
René J.


________________________________
From: Mitchell Jean A <JMitchell <@t> uwhealth.org>
To: 'Rene J Buesa' <rjbuesa <@t> yahoo.com>; P.E. Visser <pev <@t> xs4all.nl>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Monday, September 9, 2013 3:49 PM
Subject: RE: [Histonet] Oil red O


I believe the question is:  what does the "O" in Oil Red O stand for?


Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital & Clinics
Neuromuscular Laboratory 
600 Highland Avenue
Madison, WI  53792-5132 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, September 09, 2013 2:45 PM
To: P.E. Visser; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Oil red O

Do you mean that you were asked to do Oil Red O (ORO) stain?
It is described in any technology book.
I piece of advise: stain the nuclei with hametoxyline first and after that stain with ORO René J.


________________________________
From: P.E. Visser <pev <@t> xs4all.nl>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Monday, September 9, 2013 3:40 PM
Subject: [Histonet] Oil red O


Hi all

I was requested where the O stands for. who has any suggestion.



Regards Piet Visser 

Histotech Bronovo The Netherlands





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