[Histonet] unsubscribe

Mon Sep 9 14:08:23 CDT 2013

Jeffrey S. Silverman HT HTL QIHC (ASCP)
Operations Manager
Anatomic Pathology Specimen Processing,
Laboratory Safety Officer

Lenox Hill Hospital
12 Uris- Anatomic Pathology
100 East 77th Street
New York, New York 10075

office- 212 434 6673
cell-631 375 4505
email:  jsilverman <@t> nshs.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, September 09, 2013 1:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 118, Issue 10

Send Histonet mailing list submissions to
        histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."

Today's Topics:

   1. RE: Cell Block Preparation (Cartun, Richard)
   2. Re: Unsubscribe, Chapter 195 (Emily Sours)
   3. (no subject) (Martin, Erin)
   4. RE: (no subject) (Manfre, Philip)
   5. RE: (no subject) (Marcum, Pamela A)
   6. RELIA Hot Histology Job Alert Histology Supervisor        needed in
      North Florida. (Pam Barker)
   7. Re: Unsubscribe, Chapter 195 (Lee & Peggy Wenk)
   8. Re: Unsubscribe, Chapter 195 (nmhisto <@t> comcast.net)
   9. ICC on previously Wright stained smears (Susan Foreman)
  10. Re: ICC on previously Wright stained smears (Rene J Buesa)
  11. Re: Unsubscribe, Chapter 195 (dusko trajkovic)
  12. Re: (no subject) (Jennifer MacDonald)
  13. RE: FISH enumeration (Sue Hunter)

----------------------------------------------------------------------

Message: 1
Date: Mon, 9 Sep 2013 00:20:12 +0000
From: "Cartun, Richard" <Richard.Cartun <@t> hhchealth.org>
Subject: RE: [Histonet] Cell Block Preparation
To: Ann Specian <thisisann <@t> aol.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9215BD4B0BA1B44D962A71C758B68D2E018820D5 <@t> HHCEXCHMB05.hhcsystem.org>
Content-Type: text/plain; charset="us-ascii"

We borrowed a wonderful cell block procedure from Windham Community Memorial Hospital here in CT several years ago.  The specimen is collected fresh in sterile saline or RPMI and then centrifuged to concentrate the cells.  Plasma and then thrombin are added to form a clot.  The clot is then fixed in formalin and processed in our Histology Laboratory.  I will send the procedure to anyone who is interested.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Ann Specian [thisisann <@t> aol.com]
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation

I am getting complaints in regard to "insufficient" cell blocks.  We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper.

Does anyone have a more current technique which renders better cellularity?

Also, do you know which renders a better cell block:  a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?

Thanks,
Ann
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 2
Date: Mon, 9 Sep 2013 07:32:25 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Unsubscribe, Chapter 195
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <CAP=XX1zBwxubqfSKbeX7oz4=AVMWNRDTA3+gZ4jYa9xWbUPEJw <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Excellent idea!!

"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"

On Sat, Sep 7, 2013 at 4:57 PM, Amos Brooks <amosbrooks <@t> gmail.com> wrote:

> Hi,
>      I do not disagree at all. in fact, I think it would make for an
> interesting management tool. If you have the nicest CV around, but a simple
> Histonet archive search for your name + "unsubscribe" shows that you can't
> follow simple instructions, it says something about your communication
> skills and raises interesting questions about weather you are a good
> employment candidate. One might also look for the various incarnations of
> misspelled attempts at unsubscribing such as "unscribe" and "unsiscribe".
>
> Just sayin'
> Amos
>
> On Fri, Sep 6, 2013 at 1:00 PM,
> <histonet-request <@t> lists.utsouthwestern.edu>wrote:
>
> > Message: 6
> > Date: Fri, 6 Sep 2013 01:56:37 +0000 (UTC)
> > From: nmhisto <@t> comcast.net
> > Subject: [Histonet] Unsubscribe, Chapter 195
> > To: HISTONET  <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >         <
> >
> 1200426006.1029812.1378432597832.JavaMail.root <@t> sz0075a.emeryville.ca.mail.comcast.net
> > >
> >
> > Content-Type: text/plain; charset=utf-8
> >
> > It is a concern that members of our??  technically-oriented career field
> > ?? have a difficult time understanding the method for unsubscribing to
> > Histonet.??  There is an ?? almost- daily posting to "unsubscribe", despite
> > the fact that this subject has been addressed literally hundreds of
> > times.??  When one "joins" Histonet, instructions are provided, should be
> > printed out for reference and used?? if the subscriber decides to leave
> the
> > group.??  We are required to be knowledgeable on all manner of technical
> > routines requiring detailed instructions and??  Histonet is no less clear
> in
> > the methods for joining and "un-joining".??  Use them, please.??  Fire
> away -
> > I'm retired and I can take the flak!??  I do miss my microtome, though...
> >
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

------------------------------

Message: 3
Date: Mon, 9 Sep 2013 13:28:29 +0000
From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Subject: [Histonet] (no subject)
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Cc: "Naujokas, Agne" <Agne.Naujokas <@t> ucsfmedctr.org>, "Meier,    Melissa"
        <Melissa.Meier <@t> ucsfmedctr.org>
Message-ID: <24B7B291CC88D04AB663958E77A1F59D18144C <@t> ex09.net.ucsf.edu>
Content-Type: text/plain; charset=iso-8859-1

Good morning all!

One of our fellows emailed me a question that she came across while studying for her boards:

"I'm studying for my board exam and came across questions re: paraffin embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing something?  Does anyone have a clear cut answer to this?

Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited.  If you receive this in error, please contact the sender and delete the material from any computer.

------------------------------

Message: 4
Date: Mon, 9 Sep 2013 09:42:48 -0400
From: "Manfre, Philip" <philip_manfre <@t> merck.com>
Subject: RE: [Histonet] (no subject)
To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>, histonet
        <histonet <@t> lists.utsouthwestern.edu>
Cc: "Naujokas, Agne" <Agne.Naujokas <@t> ucsfmedctr.org>, "Meier,    Melissa"
        <Melissa.Meier <@t> ucsfmedctr.org>
Message-ID:
        <558A4571351D0C42BD923F403F4198C4B2701AE678 <@t> USCTMXP51014.merck.com>
Content-Type: text/plain; charset="us-ascii"

It's a bad question.  Generally the "best" temperature is probably between 55 and 65, depending on the paraffin.  This would fall between the two latter answers.  Hmm....  What to do?

Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre <@t> merck.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 9:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying for her boards:

"I'm studying for my board exam and came across questions re: paraffin embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing something?  Does anyone have a clear cut answer to this?

Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited.  If you receive this in error, please contact the sender and delete the material from any computer.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------

Message: 5
Date: Mon, 9 Sep 2013 13:50:06 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: RE: [Histonet] (no subject)
To: "'Martin, Erin'" <Erin.Martin <@t> ucsf.edu>, histonet
        <histonet <@t> lists.utsouthwestern.edu>
Cc: "Naujokas, Agne" <Agne.Naujokas <@t> ucsfmedctr.org>, "Meier,    Melissa"
        <Melissa.Meier <@t> ucsfmedctr.org>
Message-ID:
        <41D3A1AF6FEF0643BDC89E0516A6EA32D3157DDE <@t> Mail2Node2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"

The last answer will cover most paraffins used routinely and may cover microwave also.  Temperatures below 58C are often melt points however; they may not be a complete liquid or have slightly increased viscosity.

Pam Marcum
UAMS

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 8:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying for her boards:

"I'm studying for my board exam and came across questions re: paraffin embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing something?  Does anyone have a clear cut answer to this?

Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited.  If you receive this in error, please contact the sender and delete the material from any computer.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

----------------------------------------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.

------------------------------

Message: 6
Date: Mon, 9 Sep 2013 10:03:12 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Hot Histology Job Alert Histology Supervisor
        needed in North Florida.
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003f01cead65$52b52b30$f81f8190$@earthlink.net>
Content-Type: text/plain;       charset="us-ascii"

Hi Histonetters!!
I hope you all had a great weekend!! And I hope your teams NCAA and NFL did
better than mine did!!  I wanted to take a minute and tell you about a
position I recently was engaged on that I am very excited about.
RELIA has been engaged to recruit for a histology supervisor for a private
pathology lab located in North Florida.  This is a busy full staffed private
full service pathology lab which processes all types of tissue and runs a
full menu of special stains and IHC.  The ideal candidate for my client will
have at least 5 years of supervisory experience in a histology lab. HT/HTL
and Florida Supervisor license or elig are required.  My client offers an
excellent salary, benefits and relocation assistance.  For more information
please contact me - Pam Barker toll free at 866-607-3542 until 5pm EST or
anytime on my cell phone at 407-353-5070 or via email at
relia1 <@t> earthlink.net to set up a time to talk.

If you know of someone who might be interested remember RELIA offers a
referral bonus.  If we place someone you refer you will earn a referral
bonus.
Have a great Monday!!!

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker

Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.com <http://www.facebook.com/PamBarkerRELIA> /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia

------------------------------

Message: 7
Date: Mon, 9 Sep 2013 10:47:55 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] Unsubscribe, Chapter 195
To: "Manfre, Philip" <philip_manfre <@t> merck.com>, <nmhisto <@t> comcast.net>,
        "HISTONET" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A5C7F7079C9A4D71B577654C9383BD5E <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="UTF-8";
        reply-type=original

I'm going to wade in, not as someone who has posted numerous times on how to
unsubscribe, but as someone assessing it from a "risk assessment"
evaluation.

If there is a lab task that is consistently being done wrong, by many
different people, it is usually NOT the fault of the people. It is either a
training issue, or a process problem. So we either have to do a better job
training and re-training, or we need to change how the process/procedure is
being done.

With the Histonet email, since people are constantly joining, often for a
day or two, we can't really improve the "training" aspect. Yes, there are
instructions when we first join, to print off/save how to subscribe or
unsubscribe or change personal information, etc. But (be honest) how many of
us pay attention to these types of instructions when we sign up to be a
"member" of a credit card or a on-line department store or an on-line book
store or other email lists? Most people do not. So we know that this type of
"training" is not effective. But we really can't do a one-on-one type of
training session for each person who signs on to Histonet. Therefore,
improving the training is not the answer.

The answer lies in modifying the process. Look at the bottom of those emails
from credit cards or hotels or department stores that you have signed up
with. There is usually a line that says "If you no longer wish to receive
these emails, click on this link and follow the instructions".

Add to that, various email lists have various methods on how to unsubscribe,
which can involve a link, or putting the word unsubscribe in the subject, or
putting the word unsubscribe in the message.

Histonet has a link at the bottom, but no instructions. So it's not clear to
click on the link to unsubscribe, nor is there any mention whether one of
the other unsubscribing methods would work. I therefore believe the Histonet
unsubscribing procedure has a process problem, that could be easily fixed.

As for the fact that how to unsubscribe has been explained 5,391+ times in
the past does not help the person who signed up over the weekend, and as of
today, decided that Histonet is not what they need. This new person has not
seen the previous requests for help with unsubscribing, nor the answers on
how to do it. Again, this is a process problem.

Is there any way Histonet can get some clearer instructions at the bottom of
each email, on how to unsubscribe, either permanently or temporarily while
on vacation? Such as saying "To unsubscribe, click on the link below, and
follow the instructions at the bottom of the next webpage".

Let's not yell at the people trying to unsubscribe. Let's work on improving
the unsubscribing process, so we don't get these requests.

Peggy A. Wenk, HTL(ASCP)SLS

-----Original Message-----
From: Manfre, Philip
Sent: Friday, September 06, 2013 7:30 AM
To: nmhisto <@t> comcast.net ; HISTONET
Subject: RE: [Histonet] Unsubscribe, Chapter 195

Trained professionals should know by now that if you want to unsubscribe,
you must type in all caps - UNSUBSCRIBE

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre <@t> merck.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
nmhisto <@t> comcast.net
Sent: Thursday, September 05, 2013 9:57 PM
To: HISTONET
Subject: [Histonet] Unsubscribe, Chapter 195

It is a concern that members of our  technically-oriented career field  have
a difficult time understanding the method for unsubscribing to Histonet.
There is an  almost- daily posting to "unsubscribe", despite the fact that
this subject has been addressed literally hundreds of times.  When one
"joins" Histonet, instructions are provided, should be printed out for
reference and used if the subscriber decides to leave the group.  We are
required to be knowledgeable on all manner of technical routines requiring
detailed instructions and  Histonet is no less clear in the methods for
joining and "un-joining".  Use them, please.  Fire away - I'm retired and I
can take the flak!  I do miss my microtome, though...
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 8
Date: Mon, 9 Sep 2013 14:58:09 +0000 (UTC)
From: nmhisto <@t> comcast.net
Subject: Re: [Histonet] Unsubscribe, Chapter 195
To: Lee & Peggy Wenk <lpwenk <@t> sbcglobal.net>
Cc: HISTONET <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <670394625.1111582.1378738689536.JavaMail.root <@t> sz0075a.emeryville.ca.mail.comcast.net>

Content-Type: text/plain; charset=utf-8

I??was happy to read your thoughtful evaluation of the problem and, after having read through it, wonder why I hadn't thought of it before!?? Perhaps a little "tweaking" by the listserv management would make this process much more clear.?? The sign-on page of information about Histonet is rather lengthy (rightly so) but perhaps it's time for a little updating.?? Thank you, Peggy, for your thoughts and proposed solution.

----- Original Message -----
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
To: "Philip Manfre" <philip_manfre <@t> merck.com>, nmhisto <@t> comcast.net, "HISTONET" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, September 9, 2013 8:47:55 AM
Subject: Re: [Histonet] Unsubscribe, Chapter 195